OTOF cDNA ORF clone, Bos taurus(cattle)

The following OTOF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OTOF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa39419 NM_001144107.1
Latest version!
Bos taurus otoferlin (OTOF), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa24250 XM_005213063.3
Latest version!
Bos taurus otoferlin (OTOF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa91931 XM_015473545.2
Latest version!
Bos taurus otoferlin (OTOF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa91932 XM_015473546.2
Latest version!
Bos taurus otoferlin (OTOF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
View Old Accession Versions >>
OBa91931 XM_015473545.1 Bos taurus otoferlin (OTOF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa91932 XM_015473546.1 Bos taurus otoferlin (OTOF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa39419
    Clone ID Related Accession (Same CDS sequence) NM_001144107.1
    Accession Version NM_001144107.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product otoferlin
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000011.1. On Jan 22, 2009 this sequence version replaced XM_869533.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145437, SAMN03145443 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT
    GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG
    ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG
    ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC
    GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC
    CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG
    CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG
    GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC
    GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA
    GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC
    ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC
    GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC
    TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC
    GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC
    GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG
    GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT
    GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG
    CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG
    GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC
    GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG
    GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC
    TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC
    GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA
    ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC
    GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG
    GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG
    GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC
    TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC
    CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA
    GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT
    CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG
    GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC
    GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT
    GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC
    AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGCTGC CAGGGAAGCG GGGCTTCGGC
    TCGGCAGGCT GGACAGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG TCTAAGCAAG
    CAGCGCAAGG ACTTCCTGTG TGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG
    GGCCTGGGCC TACTCTCCTT CCCGCCCATC AGCCTGGTCT ACACTAAGAA GCAGGCCTTC
    CAGCTCCGCG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CACCGGCCTC
    TCAGACCCCT TTGCCCGTGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTACTGAAT
    GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTACGGT
    GAAGCTCATG AGCTGCGGGA CGATCCCCCC ATCATTGTTA TCGAAATCTA CGACCAGGAC
    ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCGCTGGT GAAGATGGCC
    GACGAGGCGT ACTGCCCACC CCGCTTCCCG CCCCAGCTGG AGTACTATCA GATCTACCGG
    GGCAATGCCA CGGCCGGGGA CCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGACCAGCA
    GGGAAGGCCG ACCTGCCGCC GATCAACGGC CCAGTGGACA TGGACCGAGG TCCCATCATG
    CCTGTGCCTG TGGGCATCCG ACCCGTGCTT AGCAAGTACC GGGTGGAGGT GCTGTTCTGG
    GGCCTGCGAG ACCTGAAGCG GGTAAACCTG GCCCAGGTGG ACAGGCCGCG GGTGGACATC
    GAGTGCGCGG GGAAGGGGGT GCAGTCCTCC CTGATCCACA ATTACAAGAA GAACCCCAAC
    TTCAGCACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT CCTGCACCCG
    CCCTTGAACA TCCGCGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTCGGCTCC
    CACGCTGTCA GCTCCCTGCG GCGCTTCATC TACCGACCCC CGGACCGCTC AGCTCCCAAC
    TGGAACACTT CGGGCAGGCT CCTCCAGGGC CGCCGTGGGC TGTACAGTGG GGGCCCCTCC
    TCTCGCCTCA CAGGGGAGGT CGTGGTGAGC ATGGAGCCGG AGGTGCCCAT CAAGAAGCTG
    GAGACCATGG TGAAGCTGGA CGCGACTTCC GATGCCGTCA TCAAGGTGGA TGTGGCTGAG
    GAAGAGAAGG AGAGGAAGAA GAAGAAGAAG AAGGGCAGCT CAGATGAGCC GGAGGAGGAG
    GAGCCAGATG AGAGTATGTT GGACTGGTGG TCCAAGTACT TTGCCTCCAT TGACACCATG
    AAAGAGCAAA TTCGACAACA AGAAATCTCA GGAATTGACC TGGAGGAGAA GGAGGAGATG
    GACAACACTG AAGGCCTGAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAG GGCTGCAAAG
    GAGGAGAAGA AGAAAAGAAC CCAGAGCCTA GGCCCTGGCC AGGGGTCGGA GGCCCCCGAG
    AAGAGGAAAC CCAAGATTGA TGAGCTCAAG GTGTACCCCA AGGAGCTGGA GTCAGAGTTC
    GACAACTTCG AGGACTGGCT GCACACGTTC AACCTGCTTC GGGGGAAGAC GGGGGACGAC
    GAGGATGGCG GGACCGAGGA GGAGCGCATT GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG
    TACAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC
    ATGTTCCAGG GCATCCCCAG CAATGACCCC ATCAACGTGC TGGTCCGGGT CTACGTGGTC
    CGGGCCACGG ACCTGCACCC CGCAGACATC AACGGCAAAG CTGACCCGTA CATCGCCATC
    CGGCTAGGCA AGACTGATAT CCGTGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCC
    GTCTTTGGGA AGTCCTTTGA CATCGAAGCC TGCTTCCCCA TGGAATCCAT GCTGACGGTG
    GCCGTGTACG ACTGGGATCT GGTAGGCACT GATGACCTCA TTGGGGAAAC CAAGATCGAC
    CTGGAAAACC GCTTCTACAG CAAGCACCGC GCCACCTGCG GCATCGCCCA GACCTACTCC
    GTACACGGCT ACAATATCTG GCGGGACCCT ATGAAGCCCA GCCAGATCCT GACCCGCCTC
    TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTCGGGCCCC CCGGGAGAGT GAAGGTGTCC
    AACCGCATCT TCACAGGGCC CTCTGAGATC GAGGACGAGA ATGGCCAGAG GAAGCCCACA
    GAAGAGCACG TGGCACTGCT GGCCCTGAGA CACTGGGAGG ACATCCCCCG CGTGGGCTGT
    CGCCTGGTGC CCGAACATGT GGAGACACGG CCGCTGCTCA ACCCCGACAA GCCGGGCATC
    GAACAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTTCCCA TGGACATGCC GTCCCCCGGG
    ACACCTCTGG ACATCTCCCC TCGGAAGCCC AAGAAGTACG AGCTGAGGGT CATCGTGTGG
    AACACGGATG AGGTGGTCTT GGAAGATGAC GACTTCTTCA CTGGGGAGAA GTCCAGCGAC
    ATTTTCGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC AGACGTCCAC
    TACCACTCCC TCACGGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC
    CTGGCTGCCG AGGAGAAGAT AGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG
    ACCGAATACA AGATCCCCGC ACGGCTCACC CTGCAGATCT GGGACGCCGA CCACTTCTCT
    GCCGATGACT TCCTGGGGGC CATCGAGCTG GACCTGAATC GCTTCCCTCG GGGCGCGAAA
    ACGGCCAAGC AGTGCACCAT GGAGATGGCC ACAGGGGAGG TGGATGTGCC CCTGGTTTCC
    ATCTTCAAGC AGAAGCGTGT CAAAGGCTGG TGGCCCCTCC TAGCCCGCAA CGAGAATGAC
    GAGTTTGAGC TCACGGGCAA AGTGGAAGCA GAGTTGCATC TACTCACCGC CGAGGAGGCG
    GAAAAGAATC CCGTGGGCCT GGCCCGCAAT GAACCTGATC CCCTAGAGAA ACCCAACCGG
    CCCGACACCG CCTTCGTCTG GTTCCTCAAC CCCCTCAAGT CCATCAAGTA CCTCATCTGC
    ACGCGGTACA AGTGGCTCAT CATCAAGATC GTGTTGGCGC TGCTGGGACT GCTCATGCTG
    GCGCTCTTCC TCTACAGCCT CCCCGGTTAC ATGGTCAAGA AGCTCTTGGG GGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137579.1
    CDS1..5997
    Translation

    Target ORF information:

    RefSeq Version NM_001144107.1
    Organism Bos taurus(cattle)
    Definition Bos taurus otoferlin (OTOF), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144107.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT
    GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG
    ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG
    ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC
    GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC
    CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG
    CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG
    GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC
    GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA
    GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC
    ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC
    GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC
    TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC
    GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC
    GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG
    GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT
    GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG
    CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG
    GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC
    GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG
    GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC
    TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC
    GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA
    ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC
    GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG
    GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG
    GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC
    TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC
    CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA
    GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT
    CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG
    GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC
    GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT
    GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC
    AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGCTGC CAGGGAAGCG GGGCTTCGGC
    TCGGCAGGCT GGACAGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG TCTAAGCAAG
    CAGCGCAAGG ACTTCCTGTG TGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG
    GGCCTGGGCC TACTCTCCTT CCCGCCCATC AGCCTGGTCT ACACTAAGAA GCAGGCCTTC
    CAGCTCCGCG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CACCGGCCTC
    TCAGACCCCT TTGCCCGTGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTACTGAAT
    GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTACGGT
    GAAGCTCATG AGCTGCGGGA CGATCCCCCC ATCATTGTTA TCGAAATCTA CGACCAGGAC
    ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCGCTGGT GAAGATGGCC
    GACGAGGCGT ACTGCCCACC CCGCTTCCCG CCCCAGCTGG AGTACTATCA GATCTACCGG
    GGCAATGCCA CGGCCGGGGA CCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGACCAGCA
    GGGAAGGCCG ACCTGCCGCC GATCAACGGC CCAGTGGACA TGGACCGAGG TCCCATCATG
    CCTGTGCCTG TGGGCATCCG ACCCGTGCTT AGCAAGTACC GGGTGGAGGT GCTGTTCTGG
    GGCCTGCGAG ACCTGAAGCG GGTAAACCTG GCCCAGGTGG ACAGGCCGCG GGTGGACATC
    GAGTGCGCGG GGAAGGGGGT GCAGTCCTCC CTGATCCACA ATTACAAGAA GAACCCCAAC
    TTCAGCACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT CCTGCACCCG
    CCCTTGAACA TCCGCGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTCGGCTCC
    CACGCTGTCA GCTCCCTGCG GCGCTTCATC TACCGACCCC CGGACCGCTC AGCTCCCAAC
    TGGAACACTT CGGGCAGGCT CCTCCAGGGC CGCCGTGGGC TGTACAGTGG GGGCCCCTCC
    TCTCGCCTCA CAGGGGAGGT CGTGGTGAGC ATGGAGCCGG AGGTGCCCAT CAAGAAGCTG
    GAGACCATGG TGAAGCTGGA CGCGACTTCC GATGCCGTCA TCAAGGTGGA TGTGGCTGAG
    GAAGAGAAGG AGAGGAAGAA GAAGAAGAAG AAGGGCAGCT CAGATGAGCC GGAGGAGGAG
    GAGCCAGATG AGAGTATGTT GGACTGGTGG TCCAAGTACT TTGCCTCCAT TGACACCATG
    AAAGAGCAAA TTCGACAACA AGAAATCTCA GGAATTGACC TGGAGGAGAA GGAGGAGATG
    GACAACACTG AAGGCCTGAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAG GGCTGCAAAG
    GAGGAGAAGA AGAAAAGAAC CCAGAGCCTA GGCCCTGGCC AGGGGTCGGA GGCCCCCGAG
    AAGAGGAAAC CCAAGATTGA TGAGCTCAAG GTGTACCCCA AGGAGCTGGA GTCAGAGTTC
    GACAACTTCG AGGACTGGCT GCACACGTTC AACCTGCTTC GGGGGAAGAC GGGGGACGAC
    GAGGATGGCG GGACCGAGGA GGAGCGCATT GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG
    TACAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC
    ATGTTCCAGG GCATCCCCAG CAATGACCCC ATCAACGTGC TGGTCCGGGT CTACGTGGTC
    CGGGCCACGG ACCTGCACCC CGCAGACATC AACGGCAAAG CTGACCCGTA CATCGCCATC
    CGGCTAGGCA AGACTGATAT CCGTGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCC
    GTCTTTGGGA AGTCCTTTGA CATCGAAGCC TGCTTCCCCA TGGAATCCAT GCTGACGGTG
    GCCGTGTACG ACTGGGATCT GGTAGGCACT GATGACCTCA TTGGGGAAAC CAAGATCGAC
    CTGGAAAACC GCTTCTACAG CAAGCACCGC GCCACCTGCG GCATCGCCCA GACCTACTCC
    GTACACGGCT ACAATATCTG GCGGGACCCT ATGAAGCCCA GCCAGATCCT GACCCGCCTC
    TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTCGGGCCCC CCGGGAGAGT GAAGGTGTCC
    AACCGCATCT TCACAGGGCC CTCTGAGATC GAGGACGAGA ATGGCCAGAG GAAGCCCACA
    GAAGAGCACG TGGCACTGCT GGCCCTGAGA CACTGGGAGG ACATCCCCCG CGTGGGCTGT
    CGCCTGGTGC CCGAACATGT GGAGACACGG CCGCTGCTCA ACCCCGACAA GCCGGGCATC
    GAACAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTTCCCA TGGACATGCC GTCCCCCGGG
    ACACCTCTGG ACATCTCCCC TCGGAAGCCC AAGAAGTACG AGCTGAGGGT CATCGTGTGG
    AACACGGATG AGGTGGTCTT GGAAGATGAC GACTTCTTCA CTGGGGAGAA GTCCAGCGAC
    ATTTTCGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC AGACGTCCAC
    TACCACTCCC TCACGGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC
    CTGGCTGCCG AGGAGAAGAT AGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG
    ACCGAATACA AGATCCCCGC ACGGCTCACC CTGCAGATCT GGGACGCCGA CCACTTCTCT
    GCCGATGACT TCCTGGGGGC CATCGAGCTG GACCTGAATC GCTTCCCTCG GGGCGCGAAA
    ACGGCCAAGC AGTGCACCAT GGAGATGGCC ACAGGGGAGG TGGATGTGCC CCTGGTTTCC
    ATCTTCAAGC AGAAGCGTGT CAAAGGCTGG TGGCCCCTCC TAGCCCGCAA CGAGAATGAC
    GAGTTTGAGC TCACGGGCAA AGTGGAAGCA GAGTTGCATC TACTCACCGC CGAGGAGGCG
    GAAAAGAATC CCGTGGGCCT GGCCCGCAAT GAACCTGATC CCCTAGAGAA ACCCAACCGG
    CCCGACACCG CCTTCGTCTG GTTCCTCAAC CCCCTCAAGT CCATCAAGTA CCTCATCTGC
    ACGCGGTACA AGTGGCTCAT CATCAAGATC GTGTTGGCGC TGCTGGGACT GCTCATGCTG
    GCGCTCTTCC TCTACAGCCT CCCCGGTTAC ATGGTCAAGA AGCTCTTGGG GGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa24250
    Clone ID Related Accession (Same CDS sequence) XM_005213063.3
    Accession Version XM_005213063.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5937bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product otoferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005213063.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT
    GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG
    ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG
    ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC
    GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC
    CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG
    CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG
    GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC
    GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA
    GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC
    ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC
    GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC
    TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC
    GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC
    GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG
    GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT
    GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG
    CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG
    GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC
    GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG
    GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC
    TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC
    GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA
    ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC
    GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG
    GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG
    GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC
    TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC
    CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA
    GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT
    CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG
    GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC
    GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT
    GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC
    AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGCTGC CAGGGAAGCG GGGCTTCGGC
    TCGGCAGGCT GGACAGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG TCTAAGCAAG
    CAGCGCAAGG ACTTCCTGTG TGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG
    GGCCTGGGCC TACTCTCCTT CCCGCCCATC AGCCTGGTCT ACACTAAGAA GCAGGCCTTC
    CAGCTCCGCG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CACCGGCCTC
    TCAGACCCCT TTGCCCGTGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTACTGAAT
    GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTACGGT
    GAAGCTCATG AGCTGCGGGA CGATCCCCCC ATCATTGTTA TCGAAATCTA CGACCAGGAC
    ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCGCTGGT GAAGATGGCC
    GACGAGGCGT ACTGCCCACC CCGCTTCCCG CCCCAGCTGG AGTACTATCA GATCTACCGG
    GGCAATGCCA CGGCCGGGGA CCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGACCAGCA
    GGGAAGGCCG ACCTGCCGCC GATCAACGGC CCAGTGGACA TGGACCGAGG TCCCATCATG
    CCTGTGCCTG TGGGCATCCG ACCCGTGCTT AGCAAGTACC GGGTGGAGGT GCTGTTCTGG
    GGCCTGCGAG ACCTGAAGCG GGTAAACCTG GCCCAGGTGG ACAGGCCGCG GGTGGACATC
    GAGTGCGCGG GGAAGGGGGT GCAGTCCTCC CTGATCCACA ATTACAAGAA GAACCCCAAC
    TTCAGCACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT CCTGCACCCG
    CCCTTGAACA TCCGCGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTCGGCTCC
    CACGCTGTCA GCTCCCTGCG GCGCTTCATC TACCGACCCC CGGACCGCTC AGCTCCCAAC
    TGGAACACTT CGGGGGAGGT CGTGGTGAGC ATGGAGCCGG AGGTGCCCAT CAAGAAGCTG
    GAGACCATGG TGAAGCTGGA CGCGACTTCC GATGCCGTCA TCAAGGTGGA TGTGGCTGAG
    GAAGAGAAGG AGAGGAAGAA GAAGAAGAAG AAGGGCAGCT CAGATGAGCC GGAGGAGGAG
    GAGCCAGATG AGAGTATGTT GGACTGGTGG TCCAAGTACT TTGCCTCCAT TGACACCATG
    AAAGAGCAAA TTCGACAACA AGAAATCTCA GGAATTGACC TGGAGGAGAA GGAGGAGATG
    GACAACACTG AAGGCCTGAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAG GGCTGCAAAG
    GAGGAGAAGA AGAAAAGAAC CCAGAGCCTA GGCCCTGGCC AGGGGTCGGA GGCCCCCGAG
    AAGAGGAAAC CCAAGATTGA TGAGCTCAAG GTGTACCCCA AGGAGCTGGA GTCAGAGTTC
    GACAACTTCG AGGACTGGCT GCACACGTTC AACCTGCTTC GGGGGAAGAC GGGGGACGAC
    GAGGATGGCG GGACCGAGGA GGAGCGCATT GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG
    TACAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC
    ATGTTCCAGG GCATCCCCAG CAATGACCCC ATCAACGTGC TGGTCCGGGT CTACGTGGTC
    CGGGCCACGG ACCTGCACCC CGCAGACATC AACGGCAAAG CTGACCCGTA CATCGCCATC
    CGGCTAGGCA AGACTGATAT CCGTGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCC
    GTCTTTGGGA AGTCCTTTGA CATCGAAGCC TGCTTCCCCA TGGAATCCAT GCTGACGGTG
    GCCGTGTACG ACTGGGATCT GGTAGGCACT GATGACCTCA TTGGGGAAAC CAAGATCGAC
    CTGGAAAACC GCTTCTACAG CAAGCACCGC GCCACCTGCG GCATCGCCCA GACCTACTCC
    GTACACGGCT ACAATATCTG GCGGGACCCT ATGAAGCCCA GCCAGATCCT GACCCGCCTC
    TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTCGGGCCCC CCGGGAGAGT GAAGGTGTCC
    AACCGCATCT TCACAGGGCC CTCTGAGATC GAGGACGAGA ATGGCCAGAG GAAGCCCACA
    GAAGAGCACG TGGCACTGCT GGCCCTGAGA CACTGGGAGG ACATCCCCCG CGTGGGCTGT
    CGCCTGGTGC CCGAACATGT GGAGACACGG CCGCTGCTCA ACCCCGACAA GCCGGGCATC
    GAACAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTTCCCA TGGACATGCC GTCCCCCGGG
    ACACCTCTGG ACATCTCCCC TCGGAAGCCC AAGAAGTACG AGCTGAGGGT CATCGTGTGG
    AACACGGATG AGGTGGTCTT GGAAGATGAC GACTTCTTCA CTGGGGAGAA GTCCAGCGAC
    ATTTTCGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC AGACGTCCAC
    TACCACTCCC TCACGGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC
    CTGGCTGCCG AGGAGAAGAT AGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG
    ACCGAATACA AGATCCCCGC ACGGCTCACC CTGCAGATCT GGGACGCCGA CCACTTCTCT
    GCCGATGACT TCCTGGGGGC CATCGAGCTG GACCTGAATC GCTTCCCTCG GGGCGCGAAA
    ACGGCCAAGC AGTGCACCAT GGAGATGGCC ACAGGGGAGG TGGATGTGCC CCTGGTTTCC
    ATCTTCAAGC AGAAGCGTGT CAAAGGCTGG TGGCCCCTCC TAGCCCGCAA CGAGAATGAC
    GAGTTTGAGC TCACGGGCAA AGTGGAAGCA GAGTTGCATC TACTCACCGC CGAGGAGGCG
    GAAAAGAATC CCGTGGGCCT GGCCCGCAAT GAACCTGATC CCCTAGAGAA ACCCAACCGG
    CCCGACACCG CCTTCGTCTG GTTCCTCAAC CCCCTCAAGT CCATCAAGTA CCTCATCTGC
    ACGCGGTACA AGTGGCTCAT CATCAAGATC GTGTTGGCGC TGCTGGGACT GCTCATGCTG
    GCGCTCTTCC TCTACAGCCT CCCCGGTTAC ATGGTCAAGA AGCTCTTGGG GGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005213120.1
    CDS1..5937
    Translation

    Target ORF information:

    RefSeq Version XM_005213063.3
    Organism Bos taurus(cattle)
    Definition Bos taurus otoferlin (OTOF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005213063.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT
    GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG
    ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG
    ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC
    GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC
    CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG
    CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG
    GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC
    GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA
    GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC
    ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC
    GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC
    TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC
    GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC
    GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG
    GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT
    GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG
    CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG
    GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC
    GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG
    GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC
    TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC
    GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA
    ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC
    GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG
    GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG
    GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC
    TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC
    CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA
    GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT
    CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG
    GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC
    GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT
    GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC
    AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGCTGC CAGGGAAGCG GGGCTTCGGC
    TCGGCAGGCT GGACAGTGCA GGCCAAGCTG GAGCTCTACC TGTGGCTGGG TCTAAGCAAG
    CAGCGCAAGG ACTTCCTGTG TGGCCTGCCC TGCGGCTTCG AGGAGGTCAA GGCAGCCCAG
    GGCCTGGGCC TACTCTCCTT CCCGCCCATC AGCCTGGTCT ACACTAAGAA GCAGGCCTTC
    CAGCTCCGCG CCCACATGTA CCAGGCTCGC AGCCTCTTTG CTGCTGACAG CACCGGCCTC
    TCAGACCCCT TTGCCCGTGT CTTCTTCATC AACCAGAGTC AGTGCACAGA GGTACTGAAT
    GAGACCCTGT GCCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTACGGT
    GAAGCTCATG AGCTGCGGGA CGATCCCCCC ATCATTGTTA TCGAAATCTA CGACCAGGAC
    ACCATGGGCA AAGCCGACTT CATGGGCCGG ACCTTTGCCA AGCCGCTGGT GAAGATGGCC
    GACGAGGCGT ACTGCCCACC CCGCTTCCCG CCCCAGCTGG AGTACTATCA GATCTACCGG
    GGCAATGCCA CGGCCGGGGA CCTGCTGGCT GCCTTCGAGC TGCTGCAGAT TGGACCAGCA
    GGGAAGGCCG ACCTGCCGCC GATCAACGGC CCAGTGGACA TGGACCGAGG TCCCATCATG
    CCTGTGCCTG TGGGCATCCG ACCCGTGCTT AGCAAGTACC GGGTGGAGGT GCTGTTCTGG
    GGCCTGCGAG ACCTGAAGCG GGTAAACCTG GCCCAGGTGG ACAGGCCGCG GGTGGACATC
    GAGTGCGCGG GGAAGGGGGT GCAGTCCTCC CTGATCCACA ATTACAAGAA GAACCCCAAC
    TTCAGCACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT CCTGCACCCG
    CCCTTGAACA TCCGCGTGGT GGACTGCCGG GCCTTCGGCC GCTACACCCT GGTCGGCTCC
    CACGCTGTCA GCTCCCTGCG GCGCTTCATC TACCGACCCC CGGACCGCTC AGCTCCCAAC
    TGGAACACTT CGGGGGAGGT CGTGGTGAGC ATGGAGCCGG AGGTGCCCAT CAAGAAGCTG
    GAGACCATGG TGAAGCTGGA CGCGACTTCC GATGCCGTCA TCAAGGTGGA TGTGGCTGAG
    GAAGAGAAGG AGAGGAAGAA GAAGAAGAAG AAGGGCAGCT CAGATGAGCC GGAGGAGGAG
    GAGCCAGATG AGAGTATGTT GGACTGGTGG TCCAAGTACT TTGCCTCCAT TGACACCATG
    AAAGAGCAAA TTCGACAACA AGAAATCTCA GGAATTGACC TGGAGGAGAA GGAGGAGATG
    GACAACACTG AAGGCCTGAA GGGGCCGATG AAGGGCAAGG AGAAGGCAAG GGCTGCAAAG
    GAGGAGAAGA AGAAAAGAAC CCAGAGCCTA GGCCCTGGCC AGGGGTCGGA GGCCCCCGAG
    AAGAGGAAAC CCAAGATTGA TGAGCTCAAG GTGTACCCCA AGGAGCTGGA GTCAGAGTTC
    GACAACTTCG AGGACTGGCT GCACACGTTC AACCTGCTTC GGGGGAAGAC GGGGGACGAC
    GAGGATGGCG GGACCGAGGA GGAGCGCATT GTGGGCCGGT TCAAGGGCTC CCTCTGCGTG
    TACAAAGTGC CACTCCCAGA GGACGTGTCC CGGGAAGCCG GCTACGACCC CACCTACGGC
    ATGTTCCAGG GCATCCCCAG CAATGACCCC ATCAACGTGC TGGTCCGGGT CTACGTGGTC
    CGGGCCACGG ACCTGCACCC CGCAGACATC AACGGCAAAG CTGACCCGTA CATCGCCATC
    CGGCTAGGCA AGACTGATAT CCGTGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCC
    GTCTTTGGGA AGTCCTTTGA CATCGAAGCC TGCTTCCCCA TGGAATCCAT GCTGACGGTG
    GCCGTGTACG ACTGGGATCT GGTAGGCACT GATGACCTCA TTGGGGAAAC CAAGATCGAC
    CTGGAAAACC GCTTCTACAG CAAGCACCGC GCCACCTGCG GCATCGCCCA GACCTACTCC
    GTACACGGCT ACAATATCTG GCGGGACCCT ATGAAGCCCA GCCAGATCCT GACCCGCCTC
    TGCAAGGAGG GCAAAGTGGA CGGCCCCCAC TTCGGGCCCC CCGGGAGAGT GAAGGTGTCC
    AACCGCATCT TCACAGGGCC CTCTGAGATC GAGGACGAGA ATGGCCAGAG GAAGCCCACA
    GAAGAGCACG TGGCACTGCT GGCCCTGAGA CACTGGGAGG ACATCCCCCG CGTGGGCTGT
    CGCCTGGTGC CCGAACATGT GGAGACACGG CCGCTGCTCA ACCCCGACAA GCCGGGCATC
    GAACAGGGCC GCCTGGAGCT GTGGGTGGAC ATGTTTCCCA TGGACATGCC GTCCCCCGGG
    ACACCTCTGG ACATCTCCCC TCGGAAGCCC AAGAAGTACG AGCTGAGGGT CATCGTGTGG
    AACACGGATG AGGTGGTCTT GGAAGATGAC GACTTCTTCA CTGGGGAGAA GTCCAGCGAC
    ATTTTCGTGC GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AGCAGGACAC AGACGTCCAC
    TACCACTCCC TCACGGGCGA GGGCAACTTC AACTGGCGCT ACCTGTTCCC CTTCGACTAC
    CTGGCTGCCG AGGAGAAGAT AGTCATCTCC AAGAAGGAGT CCATGTTCTC CTGGGACGAG
    ACCGAATACA AGATCCCCGC ACGGCTCACC CTGCAGATCT GGGACGCCGA CCACTTCTCT
    GCCGATGACT TCCTGGGGGC CATCGAGCTG GACCTGAATC GCTTCCCTCG GGGCGCGAAA
    ACGGCCAAGC AGTGCACCAT GGAGATGGCC ACAGGGGAGG TGGATGTGCC CCTGGTTTCC
    ATCTTCAAGC AGAAGCGTGT CAAAGGCTGG TGGCCCCTCC TAGCCCGCAA CGAGAATGAC
    GAGTTTGAGC TCACGGGCAA AGTGGAAGCA GAGTTGCATC TACTCACCGC CGAGGAGGCG
    GAAAAGAATC CCGTGGGCCT GGCCCGCAAT GAACCTGATC CCCTAGAGAA ACCCAACCGG
    CCCGACACCG CCTTCGTCTG GTTCCTCAAC CCCCTCAAGT CCATCAAGTA CCTCATCTGC
    ACGCGGTACA AGTGGCTCAT CATCAAGATC GTGTTGGCGC TGCTGGGACT GCTCATGCTG
    GCGCTCTTCC TCTACAGCCT CCCCGGTTAC ATGGTCAAGA AGCTCTTGGG GGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91931
    Clone ID Related Accession (Same CDS sequence) XM_015473545.2 , XM_015473545.1
    Accession Version XM_015473545.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product otoferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473545.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT
    GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG
    ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG
    ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC
    GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC
    CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG
    CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG
    GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC
    GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA
    GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC
    ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC
    GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC
    TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC
    GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC
    GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG
    GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT
    GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG
    CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG
    GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC
    GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG
    GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC
    TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC
    GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA
    ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC
    GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG
    GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG
    GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC
    TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC
    CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA
    GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT
    CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG
    GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC
    GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT
    GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC
    AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGGTGC CGGAAGGGGA ATGGATTAGC
    AGCGGGTGGA GGGGCTGGGG GCTGGGCTAC TCTCAAGAGG TCATCTTCCT CTTGGAGGGA
    GCAGCATCCA CACCCCTGGG TCTGCCAGGC CTGAGTGGAC AAGATGCTCA GGGAAGAGCT
    AAAGAGAATA GGAAGGGGGA TCCCGCCCTG CCCATCCCCT TCTGTGCTGT GTTTCCCCAC
    TGCACAGCCC AGTGGGATGG CTGCGGTGGG GATGGGCCCC CTCTGACCAC AGCTTCTTCA
    GCTCCCTGCC CCTTCCTCAC CCCGAGGGCT CTGGCAGCTG ACCCTGAGCC CACCCCCCAG
    CTGCCAGGGA AGCGGGGCTT CGGCTCGGCA GGCTGGACAG TGCAGGCCAA GCTGGAGCTC
    TACCTGTGGC TGGGTCTAAG CAAGCAGCGC AAGGACTTCC TGTGTGGCCT GCCCTGCGGC
    TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG GGCCTACTCT CCTTCCCGCC CATCAGCCTG
    GTCTACACTA AGAAGCAGGC CTTCCAGCTC CGCGCCCACA TGTACCAGGC TCGCAGCCTC
    TTTGCTGCTG ACAGCACCGG CCTCTCAGAC CCCTTTGCCC GTGTCTTCTT CATCAACCAG
    AGTCAGTGCA CAGAGGTACT GAATGAGACC CTGTGCCCCA CCTGGGACCA GATGCTGGTG
    TTCGACAACC TGGAGCTCTA CGGTGAAGCT CATGAGCTGC GGGACGATCC CCCCATCATT
    GTTATCGAAA TCTACGACCA GGACACCATG GGCAAAGCCG ACTTCATGGG CCGGACCTTT
    GCCAAGCCGC TGGTGAAGAT GGCCGACGAG GCGTACTGCC CACCCCGCTT CCCGCCCCAG
    CTGGAGTACT ATCAGATCTA CCGGGGCAAT GCCACGGCCG GGGACCTGCT GGCTGCCTTC
    GAGCTGCTGC AGATTGGACC AGCAGGGAAG GCCGACCTGC CGCCGATCAA CGGCCCAGTG
    GACATGGACC GAGGTCCCAT CATGCCTGTG CCTGTGGGCA TCCGACCCGT GCTTAGCAAG
    TACCGGGTGG AGGTGCTGTT CTGGGGCCTG CGAGACCTGA AGCGGGTAAA CCTGGCCCAG
    GTGGACAGGC CGCGGGTGGA CATCGAGTGC GCGGGGAAGG GGGTGCAGTC CTCCCTGATC
    CACAATTACA AGAAGAACCC CAACTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGACCTC
    CCAGAGAACG AGCTCCTGCA CCCGCCCTTG AACATCCGCG TGGTGGACTG CCGGGCCTTC
    GGCCGCTACA CCCTGGTCGG CTCCCACGCT GTCAGCTCCC TGCGGCGCTT CATCTACCGA
    CCCCCGGACC GCTCAGCTCC CAACTGGAAC ACTTCGGGCA GGCTCCTCCA GGGCCGCCGT
    GGGCTGTACA GTGGGGGCCC CTCCTCTCGC CTCACAGGGG AGGTCGTGGT GAGCATGGAG
    CCGGAGGTGC CCATCAAGAA GCTGGAGACC ATGGTGAAGC TGGACGCGAC TTCCGATGCC
    GTCATCAAGG TGGATGTGGC TGAGGAAGAG AAGGAGAGGA AGAAGAAGAA GAAGAAGGGC
    AGCTCAGATG AGCCGGAGGA GGAGGAGCCA GATGAGAGTA TGTTGGACTG GTGGTCCAAG
    TACTTTGCCT CCATTGACAC CATGAAAGAG CAAATTCGAC AACAAGAAAT CTCAGGAATT
    GACCTGGAGG AGAAGGAGGA GATGGACAAC ACTGAAGGCC TGAAGGGGCC GATGAAGGGC
    AAGGAGAAGG CAAGGGCTGC AAAGGAGGAG AAGAAGAAAA GAACCCAGAG CCTAGGCCCT
    GGCCAGGGGT CGGAGGCCCC CGAGAAGAGG AAACCCAAGA TTGATGAGCT CAAGGTGTAC
    CCCAAGGAGC TGGAGTCAGA GTTCGACAAC TTCGAGGACT GGCTGCACAC GTTCAACCTG
    CTTCGGGGGA AGACGGGGGA CGACGAGGAT GGCGGGACCG AGGAGGAGCG CATTGTGGGC
    CGGTTCAAGG GCTCCCTCTG CGTGTACAAA GTGCCACTCC CAGAGGACGT GTCCCGGGAA
    GCCGGCTACG ACCCCACCTA CGGCATGTTC CAGGGCATCC CCAGCAATGA CCCCATCAAC
    GTGCTGGTCC GGGTCTACGT GGTCCGGGCC ACGGACCTGC ACCCCGCAGA CATCAACGGC
    AAAGCTGACC CGTACATCGC CATCCGGCTA GGCAAGACTG ATATCCGTGA CAAGGAGAAC
    TACATCTCCA AGCAGCTCAA CCCCGTCTTT GGGAAGTCCT TTGACATCGA AGCCTGCTTC
    CCCATGGAAT CCATGCTGAC GGTGGCCGTG TACGACTGGG ATCTGGTAGG CACTGATGAC
    CTCATTGGGG AAACCAAGAT CGACCTGGAA AACCGCTTCT ACAGCAAGCA CCGCGCCACC
    TGCGGCATCG CCCAGACCTA CTCCGTACAC GGCTACAATA TCTGGCGGGA CCCTATGAAG
    CCCAGCCAGA TCCTGACCCG CCTCTGCAAG GAGGGCAAAG TGGACGGCCC CCACTTCGGG
    CCCCCCGGGA GAGTGAAGGT GTCCAACCGC ATCTTCACAG GGCCCTCTGA GATCGAGGAC
    GAGAATGGCC AGAGGAAGCC CACAGAAGAG CACGTGGCAC TGCTGGCCCT GAGACACTGG
    GAGGACATCC CCCGCGTGGG CTGTCGCCTG GTGCCCGAAC ATGTGGAGAC ACGGCCGCTG
    CTCAACCCCG ACAAGCCGGG CATCGAACAG GGCCGCCTGG AGCTGTGGGT GGACATGTTT
    CCCATGGACA TGCCGTCCCC CGGGACACCT CTGGACATCT CCCCTCGGAA GCCCAAGAAG
    TACGAGCTGA GGGTCATCGT GTGGAACACG GATGAGGTGG TCTTGGAAGA TGACGACTTC
    TTCACTGGGG AGAAGTCCAG CGACATTTTC GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG
    GACAAGCAGG ACACAGACGT CCACTACCAC TCCCTCACGG GCGAGGGCAA CTTCAACTGG
    CGCTACCTGT TCCCCTTCGA CTACCTGGCT GCCGAGGAGA AGATAGTCAT CTCCAAGAAG
    GAGTCCATGT TCTCCTGGGA CGAGACCGAA TACAAGATCC CCGCACGGCT CACCCTGCAG
    ATCTGGGACG CCGACCACTT CTCTGCCGAT GACTTCCTGG GGGCCATCGA GCTGGACCTG
    AATCGCTTCC CTCGGGGCGC GAAAACGGCC AAGCAGTGCA CCATGGAGAT GGCCACAGGG
    GAGGTGGATG TGCCCCTGGT TTCCATCTTC AAGCAGAAGC GTGTCAAAGG CTGGTGGCCC
    CTCCTAGCCC GCAACGAGAA TGACGAGTTT GAGCTCACGG GCAAAGTGGA AGCAGAGTTG
    CATCTACTCA CCGCCGAGGA GGCGGAAAAG AATCCCGTGG GCCTGGCCCG CAATGAACCT
    GATCCCCTAG AGAAACCCAA CCGGCCCGAC ACCGCCTTCG TCTGGTTCCT CAACCCCCTC
    AAGTCCATCA AGTACCTCAT CTGCACGCGG TACAAGTGGC TCATCATCAA GATCGTGTTG
    GCGCTGCTGG GACTGCTCAT GCTGGCGCTC TTCCTCTACA GCCTCCCCGG TTACATGGTC
    AAGAAGCTCT TGGGGGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329031.1
    CDS181..6501
    Translation

    Target ORF information:

    RefSeq Version XM_015473545.2
    Organism Bos taurus(cattle)
    Definition Bos taurus otoferlin (OTOF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473545.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT
    GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG
    ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG
    ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC
    GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC
    CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG
    CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG
    GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC
    GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA
    GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC
    ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC
    GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC
    TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC
    GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC
    GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG
    GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT
    GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG
    CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG
    GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC
    GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG
    GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC
    TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC
    GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA
    ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC
    GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG
    GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG
    GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC
    TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC
    CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA
    GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT
    CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG
    GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC
    GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT
    GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC
    AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGGTGC CGGAAGGGGA ATGGATTAGC
    AGCGGGTGGA GGGGCTGGGG GCTGGGCTAC TCTCAAGAGG TCATCTTCCT CTTGGAGGGA
    GCAGCATCCA CACCCCTGGG TCTGCCAGGC CTGAGTGGAC AAGATGCTCA GGGAAGAGCT
    AAAGAGAATA GGAAGGGGGA TCCCGCCCTG CCCATCCCCT TCTGTGCTGT GTTTCCCCAC
    TGCACAGCCC AGTGGGATGG CTGCGGTGGG GATGGGCCCC CTCTGACCAC AGCTTCTTCA
    GCTCCCTGCC CCTTCCTCAC CCCGAGGGCT CTGGCAGCTG ACCCTGAGCC CACCCCCCAG
    CTGCCAGGGA AGCGGGGCTT CGGCTCGGCA GGCTGGACAG TGCAGGCCAA GCTGGAGCTC
    TACCTGTGGC TGGGTCTAAG CAAGCAGCGC AAGGACTTCC TGTGTGGCCT GCCCTGCGGC
    TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG GGCCTACTCT CCTTCCCGCC CATCAGCCTG
    GTCTACACTA AGAAGCAGGC CTTCCAGCTC CGCGCCCACA TGTACCAGGC TCGCAGCCTC
    TTTGCTGCTG ACAGCACCGG CCTCTCAGAC CCCTTTGCCC GTGTCTTCTT CATCAACCAG
    AGTCAGTGCA CAGAGGTACT GAATGAGACC CTGTGCCCCA CCTGGGACCA GATGCTGGTG
    TTCGACAACC TGGAGCTCTA CGGTGAAGCT CATGAGCTGC GGGACGATCC CCCCATCATT
    GTTATCGAAA TCTACGACCA GGACACCATG GGCAAAGCCG ACTTCATGGG CCGGACCTTT
    GCCAAGCCGC TGGTGAAGAT GGCCGACGAG GCGTACTGCC CACCCCGCTT CCCGCCCCAG
    CTGGAGTACT ATCAGATCTA CCGGGGCAAT GCCACGGCCG GGGACCTGCT GGCTGCCTTC
    GAGCTGCTGC AGATTGGACC AGCAGGGAAG GCCGACCTGC CGCCGATCAA CGGCCCAGTG
    GACATGGACC GAGGTCCCAT CATGCCTGTG CCTGTGGGCA TCCGACCCGT GCTTAGCAAG
    TACCGGGTGG AGGTGCTGTT CTGGGGCCTG CGAGACCTGA AGCGGGTAAA CCTGGCCCAG
    GTGGACAGGC CGCGGGTGGA CATCGAGTGC GCGGGGAAGG GGGTGCAGTC CTCCCTGATC
    CACAATTACA AGAAGAACCC CAACTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGACCTC
    CCAGAGAACG AGCTCCTGCA CCCGCCCTTG AACATCCGCG TGGTGGACTG CCGGGCCTTC
    GGCCGCTACA CCCTGGTCGG CTCCCACGCT GTCAGCTCCC TGCGGCGCTT CATCTACCGA
    CCCCCGGACC GCTCAGCTCC CAACTGGAAC ACTTCGGGCA GGCTCCTCCA GGGCCGCCGT
    GGGCTGTACA GTGGGGGCCC CTCCTCTCGC CTCACAGGGG AGGTCGTGGT GAGCATGGAG
    CCGGAGGTGC CCATCAAGAA GCTGGAGACC ATGGTGAAGC TGGACGCGAC TTCCGATGCC
    GTCATCAAGG TGGATGTGGC TGAGGAAGAG AAGGAGAGGA AGAAGAAGAA GAAGAAGGGC
    AGCTCAGATG AGCCGGAGGA GGAGGAGCCA GATGAGAGTA TGTTGGACTG GTGGTCCAAG
    TACTTTGCCT CCATTGACAC CATGAAAGAG CAAATTCGAC AACAAGAAAT CTCAGGAATT
    GACCTGGAGG AGAAGGAGGA GATGGACAAC ACTGAAGGCC TGAAGGGGCC GATGAAGGGC
    AAGGAGAAGG CAAGGGCTGC AAAGGAGGAG AAGAAGAAAA GAACCCAGAG CCTAGGCCCT
    GGCCAGGGGT CGGAGGCCCC CGAGAAGAGG AAACCCAAGA TTGATGAGCT CAAGGTGTAC
    CCCAAGGAGC TGGAGTCAGA GTTCGACAAC TTCGAGGACT GGCTGCACAC GTTCAACCTG
    CTTCGGGGGA AGACGGGGGA CGACGAGGAT GGCGGGACCG AGGAGGAGCG CATTGTGGGC
    CGGTTCAAGG GCTCCCTCTG CGTGTACAAA GTGCCACTCC CAGAGGACGT GTCCCGGGAA
    GCCGGCTACG ACCCCACCTA CGGCATGTTC CAGGGCATCC CCAGCAATGA CCCCATCAAC
    GTGCTGGTCC GGGTCTACGT GGTCCGGGCC ACGGACCTGC ACCCCGCAGA CATCAACGGC
    AAAGCTGACC CGTACATCGC CATCCGGCTA GGCAAGACTG ATATCCGTGA CAAGGAGAAC
    TACATCTCCA AGCAGCTCAA CCCCGTCTTT GGGAAGTCCT TTGACATCGA AGCCTGCTTC
    CCCATGGAAT CCATGCTGAC GGTGGCCGTG TACGACTGGG ATCTGGTAGG CACTGATGAC
    CTCATTGGGG AAACCAAGAT CGACCTGGAA AACCGCTTCT ACAGCAAGCA CCGCGCCACC
    TGCGGCATCG CCCAGACCTA CTCCGTACAC GGCTACAATA TCTGGCGGGA CCCTATGAAG
    CCCAGCCAGA TCCTGACCCG CCTCTGCAAG GAGGGCAAAG TGGACGGCCC CCACTTCGGG
    CCCCCCGGGA GAGTGAAGGT GTCCAACCGC ATCTTCACAG GGCCCTCTGA GATCGAGGAC
    GAGAATGGCC AGAGGAAGCC CACAGAAGAG CACGTGGCAC TGCTGGCCCT GAGACACTGG
    GAGGACATCC CCCGCGTGGG CTGTCGCCTG GTGCCCGAAC ATGTGGAGAC ACGGCCGCTG
    CTCAACCCCG ACAAGCCGGG CATCGAACAG GGCCGCCTGG AGCTGTGGGT GGACATGTTT
    CCCATGGACA TGCCGTCCCC CGGGACACCT CTGGACATCT CCCCTCGGAA GCCCAAGAAG
    TACGAGCTGA GGGTCATCGT GTGGAACACG GATGAGGTGG TCTTGGAAGA TGACGACTTC
    TTCACTGGGG AGAAGTCCAG CGACATTTTC GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG
    GACAAGCAGG ACACAGACGT CCACTACCAC TCCCTCACGG GCGAGGGCAA CTTCAACTGG
    CGCTACCTGT TCCCCTTCGA CTACCTGGCT GCCGAGGAGA AGATAGTCAT CTCCAAGAAG
    GAGTCCATGT TCTCCTGGGA CGAGACCGAA TACAAGATCC CCGCACGGCT CACCCTGCAG
    ATCTGGGACG CCGACCACTT CTCTGCCGAT GACTTCCTGG GGGCCATCGA GCTGGACCTG
    AATCGCTTCC CTCGGGGCGC GAAAACGGCC AAGCAGTGCA CCATGGAGAT GGCCACAGGG
    GAGGTGGATG TGCCCCTGGT TTCCATCTTC AAGCAGAAGC GTGTCAAAGG CTGGTGGCCC
    CTCCTAGCCC GCAACGAGAA TGACGAGTTT GAGCTCACGG GCAAAGTGGA AGCAGAGTTG
    CATCTACTCA CCGCCGAGGA GGCGGAAAAG AATCCCGTGG GCCTGGCCCG CAATGAACCT
    GATCCCCTAG AGAAACCCAA CCGGCCCGAC ACCGCCTTCG TCTGGTTCCT CAACCCCCTC
    AAGTCCATCA AGTACCTCAT CTGCACGCGG TACAAGTGGC TCATCATCAA GATCGTGTTG
    GCGCTGCTGG GACTGCTCAT GCTGGCGCTC TTCCTCTACA GCCTCCCCGG TTACATGGTC
    AAGAAGCTCT TGGGGGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91931
    Clone ID Related Accession (Same CDS sequence) XM_015473545.2 , XM_015473545.1
    Accession Version XM_015473545.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product otoferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT
    GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG
    ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG
    ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC
    GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC
    CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG
    CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG
    GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC
    GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA
    GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC
    ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC
    GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC
    TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC
    GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC
    GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG
    GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT
    GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG
    CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG
    GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC
    GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG
    GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC
    TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC
    GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA
    ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC
    GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG
    GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG
    GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC
    TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC
    CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA
    GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT
    CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG
    GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC
    GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT
    GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC
    AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGGTGC CGGAAGGGGA ATGGATTAGC
    AGCGGGTGGA GGGGCTGGGG GCTGGGCTAC TCTCAAGAGG TCATCTTCCT CTTGGAGGGA
    GCAGCATCCA CACCCCTGGG TCTGCCAGGC CTGAGTGGAC AAGATGCTCA GGGAAGAGCT
    AAAGAGAATA GGAAGGGGGA TCCCGCCCTG CCCATCCCCT TCTGTGCTGT GTTTCCCCAC
    TGCACAGCCC AGTGGGATGG CTGCGGTGGG GATGGGCCCC CTCTGACCAC AGCTTCTTCA
    GCTCCCTGCC CCTTCCTCAC CCCGAGGGCT CTGGCAGCTG ACCCTGAGCC CACCCCCCAG
    CTGCCAGGGA AGCGGGGCTT CGGCTCGGCA GGCTGGACAG TGCAGGCCAA GCTGGAGCTC
    TACCTGTGGC TGGGTCTAAG CAAGCAGCGC AAGGACTTCC TGTGTGGCCT GCCCTGCGGC
    TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG GGCCTACTCT CCTTCCCGCC CATCAGCCTG
    GTCTACACTA AGAAGCAGGC CTTCCAGCTC CGCGCCCACA TGTACCAGGC TCGCAGCCTC
    TTTGCTGCTG ACAGCACCGG CCTCTCAGAC CCCTTTGCCC GTGTCTTCTT CATCAACCAG
    AGTCAGTGCA CAGAGGTACT GAATGAGACC CTGTGCCCCA CCTGGGACCA GATGCTGGTG
    TTCGACAACC TGGAGCTCTA CGGTGAAGCT CATGAGCTGC GGGACGATCC CCCCATCATT
    GTTATCGAAA TCTACGACCA GGACACCATG GGCAAAGCCG ACTTCATGGG CCGGACCTTT
    GCCAAGCCGC TGGTGAAGAT GGCCGACGAG GCGTACTGCC CACCCCGCTT CCCGCCCCAG
    CTGGAGTACT ATCAGATCTA CCGGGGCAAT GCCACGGCCG GGGACCTGCT GGCTGCCTTC
    GAGCTGCTGC AGATTGGACC AGCAGGGAAG GCCGACCTGC CGCCGATCAA CGGCCCAGTG
    GACATGGACC GAGGTCCCAT CATGCCTGTG CCTGTGGGCA TCCGACCCGT GCTTAGCAAG
    TACCGGGTGG AGGTGCTGTT CTGGGGCCTG CGAGACCTGA AGCGGGTAAA CCTGGCCCAG
    GTGGACAGGC CGCGGGTGGA CATCGAGTGC GCGGGGAAGG GGGTGCAGTC CTCCCTGATC
    CACAATTACA AGAAGAACCC CAACTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGACCTC
    CCAGAGAACG AGCTCCTGCA CCCGCCCTTG AACATCCGCG TGGTGGACTG CCGGGCCTTC
    GGCCGCTACA CCCTGGTCGG CTCCCACGCT GTCAGCTCCC TGCGGCGCTT CATCTACCGA
    CCCCCGGACC GCTCAGCTCC CAACTGGAAC ACTTCGGGCA GGCTCCTCCA GGGCCGCCGT
    GGGCTGTACA GTGGGGGCCC CTCCTCTCGC CTCACAGGGG AGGTCGTGGT GAGCATGGAG
    CCGGAGGTGC CCATCAAGAA GCTGGAGACC ATGGTGAAGC TGGACGCGAC TTCCGATGCC
    GTCATCAAGG TGGATGTGGC TGAGGAAGAG AAGGAGAGGA AGAAGAAGAA GAAGAAGGGC
    AGCTCAGATG AGCCGGAGGA GGAGGAGCCA GATGAGAGTA TGTTGGACTG GTGGTCCAAG
    TACTTTGCCT CCATTGACAC CATGAAAGAG CAAATTCGAC AACAAGAAAT CTCAGGAATT
    GACCTGGAGG AGAAGGAGGA GATGGACAAC ACTGAAGGCC TGAAGGGGCC GATGAAGGGC
    AAGGAGAAGG CAAGGGCTGC AAAGGAGGAG AAGAAGAAAA GAACCCAGAG CCTAGGCCCT
    GGCCAGGGGT CGGAGGCCCC CGAGAAGAGG AAACCCAAGA TTGATGAGCT CAAGGTGTAC
    CCCAAGGAGC TGGAGTCAGA GTTCGACAAC TTCGAGGACT GGCTGCACAC GTTCAACCTG
    CTTCGGGGGA AGACGGGGGA CGACGAGGAT GGCGGGACCG AGGAGGAGCG CATTGTGGGC
    CGGTTCAAGG GCTCCCTCTG CGTGTACAAA GTGCCACTCC CAGAGGACGT GTCCCGGGAA
    GCCGGCTACG ACCCCACCTA CGGCATGTTC CAGGGCATCC CCAGCAATGA CCCCATCAAC
    GTGCTGGTCC GGGTCTACGT GGTCCGGGCC ACGGACCTGC ACCCCGCAGA CATCAACGGC
    AAAGCTGACC CGTACATCGC CATCCGGCTA GGCAAGACTG ATATCCGTGA CAAGGAGAAC
    TACATCTCCA AGCAGCTCAA CCCCGTCTTT GGGAAGTCCT TTGACATCGA AGCCTGCTTC
    CCCATGGAAT CCATGCTGAC GGTGGCCGTG TACGACTGGG ATCTGGTAGG CACTGATGAC
    CTCATTGGGG AAACCAAGAT CGACCTGGAA AACCGCTTCT ACAGCAAGCA CCGCGCCACC
    TGCGGCATCG CCCAGACCTA CTCCGTACAC GGCTACAATA TCTGGCGGGA CCCTATGAAG
    CCCAGCCAGA TCCTGACCCG CCTCTGCAAG GAGGGCAAAG TGGACGGCCC CCACTTCGGG
    CCCCCCGGGA GAGTGAAGGT GTCCAACCGC ATCTTCACAG GGCCCTCTGA GATCGAGGAC
    GAGAATGGCC AGAGGAAGCC CACAGAAGAG CACGTGGCAC TGCTGGCCCT GAGACACTGG
    GAGGACATCC CCCGCGTGGG CTGTCGCCTG GTGCCCGAAC ATGTGGAGAC ACGGCCGCTG
    CTCAACCCCG ACAAGCCGGG CATCGAACAG GGCCGCCTGG AGCTGTGGGT GGACATGTTT
    CCCATGGACA TGCCGTCCCC CGGGACACCT CTGGACATCT CCCCTCGGAA GCCCAAGAAG
    TACGAGCTGA GGGTCATCGT GTGGAACACG GATGAGGTGG TCTTGGAAGA TGACGACTTC
    TTCACTGGGG AGAAGTCCAG CGACATTTTC GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG
    GACAAGCAGG ACACAGACGT CCACTACCAC TCCCTCACGG GCGAGGGCAA CTTCAACTGG
    CGCTACCTGT TCCCCTTCGA CTACCTGGCT GCCGAGGAGA AGATAGTCAT CTCCAAGAAG
    GAGTCCATGT TCTCCTGGGA CGAGACCGAA TACAAGATCC CCGCACGGCT CACCCTGCAG
    ATCTGGGACG CCGACCACTT CTCTGCCGAT GACTTCCTGG GGGCCATCGA GCTGGACCTG
    AATCGCTTCC CTCGGGGCGC GAAAACGGCC AAGCAGTGCA CCATGGAGAT GGCCACAGGG
    GAGGTGGATG TGCCCCTGGT TTCCATCTTC AAGCAGAAGC GTGTCAAAGG CTGGTGGCCC
    CTCCTAGCCC GCAACGAGAA TGACGAGTTT GAGCTCACGG GCAAAGTGGA AGCAGAGTTG
    CATCTACTCA CCGCCGAGGA GGCGGAAAAG AATCCCGTGG GCCTGGCCCG CAATGAACCT
    GATCCCCTAG AGAAACCCAA CCGGCCCGAC ACCGCCTTCG TCTGGTTCCT CAACCCCCTC
    AAGTCCATCA AGTACCTCAT CTGCACGCGG TACAAGTGGC TCATCATCAA GATCGTGTTG
    GCGCTGCTGG GACTGCTCAT GCTGGCGCTC TTCCTCTACA GCCTCCCCGG TTACATGGTC
    AAGAAGCTCT TGGGGGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329031.1
    CDS123..6443
    Translation

    Target ORF information:

    RefSeq Version XM_015473545.1
    Organism Bos taurus(cattle)
    Definition Bos taurus otoferlin (OTOF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473545.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT
    GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG
    ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG
    ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC
    GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC
    CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG
    CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG
    GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC
    GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA
    GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC
    ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC
    GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC
    TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC
    GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC
    GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG
    GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT
    GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG
    CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG
    GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC
    GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG
    GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC
    TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC
    GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA
    ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC
    GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG
    GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG
    GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC
    TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC
    CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA
    GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT
    CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG
    GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC
    GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT
    GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC
    AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGGTGC CGGAAGGGGA ATGGATTAGC
    AGCGGGTGGA GGGGCTGGGG GCTGGGCTAC TCTCAAGAGG TCATCTTCCT CTTGGAGGGA
    GCAGCATCCA CACCCCTGGG TCTGCCAGGC CTGAGTGGAC AAGATGCTCA GGGAAGAGCT
    AAAGAGAATA GGAAGGGGGA TCCCGCCCTG CCCATCCCCT TCTGTGCTGT GTTTCCCCAC
    TGCACAGCCC AGTGGGATGG CTGCGGTGGG GATGGGCCCC CTCTGACCAC AGCTTCTTCA
    GCTCCCTGCC CCTTCCTCAC CCCGAGGGCT CTGGCAGCTG ACCCTGAGCC CACCCCCCAG
    CTGCCAGGGA AGCGGGGCTT CGGCTCGGCA GGCTGGACAG TGCAGGCCAA GCTGGAGCTC
    TACCTGTGGC TGGGTCTAAG CAAGCAGCGC AAGGACTTCC TGTGTGGCCT GCCCTGCGGC
    TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG GGCCTACTCT CCTTCCCGCC CATCAGCCTG
    GTCTACACTA AGAAGCAGGC CTTCCAGCTC CGCGCCCACA TGTACCAGGC TCGCAGCCTC
    TTTGCTGCTG ACAGCACCGG CCTCTCAGAC CCCTTTGCCC GTGTCTTCTT CATCAACCAG
    AGTCAGTGCA CAGAGGTACT GAATGAGACC CTGTGCCCCA CCTGGGACCA GATGCTGGTG
    TTCGACAACC TGGAGCTCTA CGGTGAAGCT CATGAGCTGC GGGACGATCC CCCCATCATT
    GTTATCGAAA TCTACGACCA GGACACCATG GGCAAAGCCG ACTTCATGGG CCGGACCTTT
    GCCAAGCCGC TGGTGAAGAT GGCCGACGAG GCGTACTGCC CACCCCGCTT CCCGCCCCAG
    CTGGAGTACT ATCAGATCTA CCGGGGCAAT GCCACGGCCG GGGACCTGCT GGCTGCCTTC
    GAGCTGCTGC AGATTGGACC AGCAGGGAAG GCCGACCTGC CGCCGATCAA CGGCCCAGTG
    GACATGGACC GAGGTCCCAT CATGCCTGTG CCTGTGGGCA TCCGACCCGT GCTTAGCAAG
    TACCGGGTGG AGGTGCTGTT CTGGGGCCTG CGAGACCTGA AGCGGGTAAA CCTGGCCCAG
    GTGGACAGGC CGCGGGTGGA CATCGAGTGC GCGGGGAAGG GGGTGCAGTC CTCCCTGATC
    CACAATTACA AGAAGAACCC CAACTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGACCTC
    CCAGAGAACG AGCTCCTGCA CCCGCCCTTG AACATCCGCG TGGTGGACTG CCGGGCCTTC
    GGCCGCTACA CCCTGGTCGG CTCCCACGCT GTCAGCTCCC TGCGGCGCTT CATCTACCGA
    CCCCCGGACC GCTCAGCTCC CAACTGGAAC ACTTCGGGCA GGCTCCTCCA GGGCCGCCGT
    GGGCTGTACA GTGGGGGCCC CTCCTCTCGC CTCACAGGGG AGGTCGTGGT GAGCATGGAG
    CCGGAGGTGC CCATCAAGAA GCTGGAGACC ATGGTGAAGC TGGACGCGAC TTCCGATGCC
    GTCATCAAGG TGGATGTGGC TGAGGAAGAG AAGGAGAGGA AGAAGAAGAA GAAGAAGGGC
    AGCTCAGATG AGCCGGAGGA GGAGGAGCCA GATGAGAGTA TGTTGGACTG GTGGTCCAAG
    TACTTTGCCT CCATTGACAC CATGAAAGAG CAAATTCGAC AACAAGAAAT CTCAGGAATT
    GACCTGGAGG AGAAGGAGGA GATGGACAAC ACTGAAGGCC TGAAGGGGCC GATGAAGGGC
    AAGGAGAAGG CAAGGGCTGC AAAGGAGGAG AAGAAGAAAA GAACCCAGAG CCTAGGCCCT
    GGCCAGGGGT CGGAGGCCCC CGAGAAGAGG AAACCCAAGA TTGATGAGCT CAAGGTGTAC
    CCCAAGGAGC TGGAGTCAGA GTTCGACAAC TTCGAGGACT GGCTGCACAC GTTCAACCTG
    CTTCGGGGGA AGACGGGGGA CGACGAGGAT GGCGGGACCG AGGAGGAGCG CATTGTGGGC
    CGGTTCAAGG GCTCCCTCTG CGTGTACAAA GTGCCACTCC CAGAGGACGT GTCCCGGGAA
    GCCGGCTACG ACCCCACCTA CGGCATGTTC CAGGGCATCC CCAGCAATGA CCCCATCAAC
    GTGCTGGTCC GGGTCTACGT GGTCCGGGCC ACGGACCTGC ACCCCGCAGA CATCAACGGC
    AAAGCTGACC CGTACATCGC CATCCGGCTA GGCAAGACTG ATATCCGTGA CAAGGAGAAC
    TACATCTCCA AGCAGCTCAA CCCCGTCTTT GGGAAGTCCT TTGACATCGA AGCCTGCTTC
    CCCATGGAAT CCATGCTGAC GGTGGCCGTG TACGACTGGG ATCTGGTAGG CACTGATGAC
    CTCATTGGGG AAACCAAGAT CGACCTGGAA AACCGCTTCT ACAGCAAGCA CCGCGCCACC
    TGCGGCATCG CCCAGACCTA CTCCGTACAC GGCTACAATA TCTGGCGGGA CCCTATGAAG
    CCCAGCCAGA TCCTGACCCG CCTCTGCAAG GAGGGCAAAG TGGACGGCCC CCACTTCGGG
    CCCCCCGGGA GAGTGAAGGT GTCCAACCGC ATCTTCACAG GGCCCTCTGA GATCGAGGAC
    GAGAATGGCC AGAGGAAGCC CACAGAAGAG CACGTGGCAC TGCTGGCCCT GAGACACTGG
    GAGGACATCC CCCGCGTGGG CTGTCGCCTG GTGCCCGAAC ATGTGGAGAC ACGGCCGCTG
    CTCAACCCCG ACAAGCCGGG CATCGAACAG GGCCGCCTGG AGCTGTGGGT GGACATGTTT
    CCCATGGACA TGCCGTCCCC CGGGACACCT CTGGACATCT CCCCTCGGAA GCCCAAGAAG
    TACGAGCTGA GGGTCATCGT GTGGAACACG GATGAGGTGG TCTTGGAAGA TGACGACTTC
    TTCACTGGGG AGAAGTCCAG CGACATTTTC GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG
    GACAAGCAGG ACACAGACGT CCACTACCAC TCCCTCACGG GCGAGGGCAA CTTCAACTGG
    CGCTACCTGT TCCCCTTCGA CTACCTGGCT GCCGAGGAGA AGATAGTCAT CTCCAAGAAG
    GAGTCCATGT TCTCCTGGGA CGAGACCGAA TACAAGATCC CCGCACGGCT CACCCTGCAG
    ATCTGGGACG CCGACCACTT CTCTGCCGAT GACTTCCTGG GGGCCATCGA GCTGGACCTG
    AATCGCTTCC CTCGGGGCGC GAAAACGGCC AAGCAGTGCA CCATGGAGAT GGCCACAGGG
    GAGGTGGATG TGCCCCTGGT TTCCATCTTC AAGCAGAAGC GTGTCAAAGG CTGGTGGCCC
    CTCCTAGCCC GCAACGAGAA TGACGAGTTT GAGCTCACGG GCAAAGTGGA AGCAGAGTTG
    CATCTACTCA CCGCCGAGGA GGCGGAAAAG AATCCCGTGG GCCTGGCCCG CAATGAACCT
    GATCCCCTAG AGAAACCCAA CCGGCCCGAC ACCGCCTTCG TCTGGTTCCT CAACCCCCTC
    AAGTCCATCA AGTACCTCAT CTGCACGCGG TACAAGTGGC TCATCATCAA GATCGTGTTG
    GCGCTGCTGG GACTGCTCAT GCTGGCGCTC TTCCTCTACA GCCTCCCCGG TTACATGGTC
    AAGAAGCTCT TGGGGGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91932
    Clone ID Related Accession (Same CDS sequence) XM_015473546.1 , XM_015473546.2
    Accession Version XM_015473546.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product otoferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT
    GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG
    ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG
    ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC
    GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC
    CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG
    CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG
    GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC
    GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA
    GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC
    ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC
    GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC
    TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC
    GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC
    GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG
    GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT
    GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG
    CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG
    GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC
    GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG
    GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC
    TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC
    GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA
    ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC
    GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG
    GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG
    GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC
    TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC
    CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA
    GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT
    CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG
    GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC
    GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT
    GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC
    AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGGTGC CGGAAGGGGA ATGGATTAGC
    AGCGGGTGGA GGGGCTGGGG GCTGGGCTAC TCTCAAGAGG TCATCTTCCT CTTGGAGGGA
    GCAGCATCCA CACCCCTGGG TCTGCCAGGC CTGAGTGGAC AAGATGCTCA GGGAAGAGCT
    AAAGAGAATA GGAAGGGGGA TCCCGCCCTG CCCATCCCCT TCTGTGCTGT GTTTCCCCAC
    TGCACAGCCC AGTGGGATGG CTGCGGTGGG GATGGGCCCC CTCTGACCAC AGCTTCTTCA
    GCTCCCTGCC CCTTCCTCAC CCCGAGGGCT CTGGCAGCTG ACCCTGAGCC CACCCCCCAG
    CTGCCAGGGA AGCGGGGCTT CGGCTCGGCA GGCTGGACAG TGCAGGCCAA GCTGGAGCTC
    TACCTGTGGC TGGGTCTAAG CAAGCAGCGC AAGGACTTCC TGTGTGGCCT GCCCTGCGGC
    TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG GGCCTACTCT CCTTCCCGCC CATCAGCCTG
    GTCTACACTA AGAAGCAGGC CTTCCAGCTC CGCGCCCACA TGTACCAGGC TCGCAGCCTC
    TTTGCTGCTG ACAGCACCGG CCTCTCAGAC CCCTTTGCCC GTGTCTTCTT CATCAACCAG
    AGTCAGTGCA CAGAGGTACT GAATGAGACC CTGTGCCCCA CCTGGGACCA GATGCTGGTG
    TTCGACAACC TGGAGCTCTA CGGTGAAGCT CATGAGCTGC GGGACGATCC CCCCATCATT
    GTTATCGAAA TCTACGACCA GGACACCATG GGCAAAGCCG ACTTCATGGG CCGGACCTTT
    GCCAAGCCGC TGGTGAAGAT GGCCGACGAG GCGTACTGCC CACCCCGCTT CCCGCCCCAG
    CTGGAGTACT ATCAGATCTA CCGGGGCAAT GCCACGGCCG GGGACCTGCT GGCTGCCTTC
    GAGCTGCTGC AGATTGGACC AGCAGGGAAG GCCGACCTGC CGCCGATCAA CGGCCCAGTG
    GACATGGACC GAGGTCCCAT CATGCCTGTG CCTGTGGGCA TCCGACCCGT GCTTAGCAAG
    TACCGGGTGG AGGTGCTGTT CTGGGGCCTG CGAGACCTGA AGCGGGTAAA CCTGGCCCAG
    GTGGACAGGC CGCGGGTGGA CATCGAGTGC GCGGGGAAGG GGGTGCAGTC CTCCCTGATC
    CACAATTACA AGAAGAACCC CAACTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGACCTC
    CCAGAGAACG AGCTCCTGCA CCCGCCCTTG AACATCCGCG TGGTGGACTG CCGGGCCTTC
    GGCCGCTACA CCCTGGTCGG CTCCCACGCT GTCAGCTCCC TGCGGCGCTT CATCTACCGA
    CCCCCGGACC GCTCAGCTCC CAACTGGAAC ACTTCGGGGG AGGTCGTGGT GAGCATGGAG
    CCGGAGGTGC CCATCAAGAA GCTGGAGACC ATGGTGAAGC TGGACGCGAC TTCCGATGCC
    GTCATCAAGG TGGATGTGGC TGAGGAAGAG AAGGAGAGGA AGAAGAAGAA GAAGAAGGGC
    AGCTCAGATG AGCCGGAGGA GGAGGAGCCA GATGAGAGTA TGTTGGACTG GTGGTCCAAG
    TACTTTGCCT CCATTGACAC CATGAAAGAG CAAATTCGAC AACAAGAAAT CTCAGGAATT
    GACCTGGAGG AGAAGGAGGA GATGGACAAC ACTGAAGGCC TGAAGGGGCC GATGAAGGGC
    AAGGAGAAGG CAAGGGCTGC AAAGGAGGAG AAGAAGAAAA GAACCCAGAG CCTAGGCCCT
    GGCCAGGGGT CGGAGGCCCC CGAGAAGAGG AAACCCAAGA TTGATGAGCT CAAGGTGTAC
    CCCAAGGAGC TGGAGTCAGA GTTCGACAAC TTCGAGGACT GGCTGCACAC GTTCAACCTG
    CTTCGGGGGA AGACGGGGGA CGACGAGGAT GGCGGGACCG AGGAGGAGCG CATTGTGGGC
    CGGTTCAAGG GCTCCCTCTG CGTGTACAAA GTGCCACTCC CAGAGGACGT GTCCCGGGAA
    GCCGGCTACG ACCCCACCTA CGGCATGTTC CAGGGCATCC CCAGCAATGA CCCCATCAAC
    GTGCTGGTCC GGGTCTACGT GGTCCGGGCC ACGGACCTGC ACCCCGCAGA CATCAACGGC
    AAAGCTGACC CGTACATCGC CATCCGGCTA GGCAAGACTG ATATCCGTGA CAAGGAGAAC
    TACATCTCCA AGCAGCTCAA CCCCGTCTTT GGGAAGTCCT TTGACATCGA AGCCTGCTTC
    CCCATGGAAT CCATGCTGAC GGTGGCCGTG TACGACTGGG ATCTGGTAGG CACTGATGAC
    CTCATTGGGG AAACCAAGAT CGACCTGGAA AACCGCTTCT ACAGCAAGCA CCGCGCCACC
    TGCGGCATCG CCCAGACCTA CTCCGTACAC GGCTACAATA TCTGGCGGGA CCCTATGAAG
    CCCAGCCAGA TCCTGACCCG CCTCTGCAAG GAGGGCAAAG TGGACGGCCC CCACTTCGGG
    CCCCCCGGGA GAGTGAAGGT GTCCAACCGC ATCTTCACAG GGCCCTCTGA GATCGAGGAC
    GAGAATGGCC AGAGGAAGCC CACAGAAGAG CACGTGGCAC TGCTGGCCCT GAGACACTGG
    GAGGACATCC CCCGCGTGGG CTGTCGCCTG GTGCCCGAAC ATGTGGAGAC ACGGCCGCTG
    CTCAACCCCG ACAAGCCGGG CATCGAACAG GGCCGCCTGG AGCTGTGGGT GGACATGTTT
    CCCATGGACA TGCCGTCCCC CGGGACACCT CTGGACATCT CCCCTCGGAA GCCCAAGAAG
    TACGAGCTGA GGGTCATCGT GTGGAACACG GATGAGGTGG TCTTGGAAGA TGACGACTTC
    TTCACTGGGG AGAAGTCCAG CGACATTTTC GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG
    GACAAGCAGG ACACAGACGT CCACTACCAC TCCCTCACGG GCGAGGGCAA CTTCAACTGG
    CGCTACCTGT TCCCCTTCGA CTACCTGGCT GCCGAGGAGA AGATAGTCAT CTCCAAGAAG
    GAGTCCATGT TCTCCTGGGA CGAGACCGAA TACAAGATCC CCGCACGGCT CACCCTGCAG
    ATCTGGGACG CCGACCACTT CTCTGCCGAT GACTTCCTGG GGGCCATCGA GCTGGACCTG
    AATCGCTTCC CTCGGGGCGC GAAAACGGCC AAGCAGTGCA CCATGGAGAT GGCCACAGGG
    GAGGTGGATG TGCCCCTGGT TTCCATCTTC AAGCAGAAGC GTGTCAAAGG CTGGTGGCCC
    CTCCTAGCCC GCAACGAGAA TGACGAGTTT GAGCTCACGG GCAAAGTGGA AGCAGAGTTG
    CATCTACTCA CCGCCGAGGA GGCGGAAAAG AATCCCGTGG GCCTGGCCCG CAATGAACCT
    GATCCCCTAG AGAAACCCAA CCGGCCCGAC ACCGCCTTCG TCTGGTTCCT CAACCCCCTC
    AAGTCCATCA AGTACCTCAT CTGCACGCGG TACAAGTGGC TCATCATCAA GATCGTGTTG
    GCGCTGCTGG GACTGCTCAT GCTGGCGCTC TTCCTCTACA GCCTCCCCGG TTACATGGTC
    AAGAAGCTCT TGGGGGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329032.1
    CDS123..6383
    Translation

    Target ORF information:

    RefSeq Version XM_015473546.1
    Organism Bos taurus(cattle)
    Definition Bos taurus otoferlin (OTOF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473546.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT
    GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG
    ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG
    ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC
    GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC
    CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG
    CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG
    GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC
    GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA
    GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC
    ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC
    GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC
    TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC
    GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC
    GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG
    GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT
    GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG
    CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG
    GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC
    GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG
    GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC
    TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC
    GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA
    ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC
    GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG
    GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG
    GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC
    TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC
    CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA
    GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT
    CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG
    GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC
    GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT
    GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC
    AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGGTGC CGGAAGGGGA ATGGATTAGC
    AGCGGGTGGA GGGGCTGGGG GCTGGGCTAC TCTCAAGAGG TCATCTTCCT CTTGGAGGGA
    GCAGCATCCA CACCCCTGGG TCTGCCAGGC CTGAGTGGAC AAGATGCTCA GGGAAGAGCT
    AAAGAGAATA GGAAGGGGGA TCCCGCCCTG CCCATCCCCT TCTGTGCTGT GTTTCCCCAC
    TGCACAGCCC AGTGGGATGG CTGCGGTGGG GATGGGCCCC CTCTGACCAC AGCTTCTTCA
    GCTCCCTGCC CCTTCCTCAC CCCGAGGGCT CTGGCAGCTG ACCCTGAGCC CACCCCCCAG
    CTGCCAGGGA AGCGGGGCTT CGGCTCGGCA GGCTGGACAG TGCAGGCCAA GCTGGAGCTC
    TACCTGTGGC TGGGTCTAAG CAAGCAGCGC AAGGACTTCC TGTGTGGCCT GCCCTGCGGC
    TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG GGCCTACTCT CCTTCCCGCC CATCAGCCTG
    GTCTACACTA AGAAGCAGGC CTTCCAGCTC CGCGCCCACA TGTACCAGGC TCGCAGCCTC
    TTTGCTGCTG ACAGCACCGG CCTCTCAGAC CCCTTTGCCC GTGTCTTCTT CATCAACCAG
    AGTCAGTGCA CAGAGGTACT GAATGAGACC CTGTGCCCCA CCTGGGACCA GATGCTGGTG
    TTCGACAACC TGGAGCTCTA CGGTGAAGCT CATGAGCTGC GGGACGATCC CCCCATCATT
    GTTATCGAAA TCTACGACCA GGACACCATG GGCAAAGCCG ACTTCATGGG CCGGACCTTT
    GCCAAGCCGC TGGTGAAGAT GGCCGACGAG GCGTACTGCC CACCCCGCTT CCCGCCCCAG
    CTGGAGTACT ATCAGATCTA CCGGGGCAAT GCCACGGCCG GGGACCTGCT GGCTGCCTTC
    GAGCTGCTGC AGATTGGACC AGCAGGGAAG GCCGACCTGC CGCCGATCAA CGGCCCAGTG
    GACATGGACC GAGGTCCCAT CATGCCTGTG CCTGTGGGCA TCCGACCCGT GCTTAGCAAG
    TACCGGGTGG AGGTGCTGTT CTGGGGCCTG CGAGACCTGA AGCGGGTAAA CCTGGCCCAG
    GTGGACAGGC CGCGGGTGGA CATCGAGTGC GCGGGGAAGG GGGTGCAGTC CTCCCTGATC
    CACAATTACA AGAAGAACCC CAACTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGACCTC
    CCAGAGAACG AGCTCCTGCA CCCGCCCTTG AACATCCGCG TGGTGGACTG CCGGGCCTTC
    GGCCGCTACA CCCTGGTCGG CTCCCACGCT GTCAGCTCCC TGCGGCGCTT CATCTACCGA
    CCCCCGGACC GCTCAGCTCC CAACTGGAAC ACTTCGGGGG AGGTCGTGGT GAGCATGGAG
    CCGGAGGTGC CCATCAAGAA GCTGGAGACC ATGGTGAAGC TGGACGCGAC TTCCGATGCC
    GTCATCAAGG TGGATGTGGC TGAGGAAGAG AAGGAGAGGA AGAAGAAGAA GAAGAAGGGC
    AGCTCAGATG AGCCGGAGGA GGAGGAGCCA GATGAGAGTA TGTTGGACTG GTGGTCCAAG
    TACTTTGCCT CCATTGACAC CATGAAAGAG CAAATTCGAC AACAAGAAAT CTCAGGAATT
    GACCTGGAGG AGAAGGAGGA GATGGACAAC ACTGAAGGCC TGAAGGGGCC GATGAAGGGC
    AAGGAGAAGG CAAGGGCTGC AAAGGAGGAG AAGAAGAAAA GAACCCAGAG CCTAGGCCCT
    GGCCAGGGGT CGGAGGCCCC CGAGAAGAGG AAACCCAAGA TTGATGAGCT CAAGGTGTAC
    CCCAAGGAGC TGGAGTCAGA GTTCGACAAC TTCGAGGACT GGCTGCACAC GTTCAACCTG
    CTTCGGGGGA AGACGGGGGA CGACGAGGAT GGCGGGACCG AGGAGGAGCG CATTGTGGGC
    CGGTTCAAGG GCTCCCTCTG CGTGTACAAA GTGCCACTCC CAGAGGACGT GTCCCGGGAA
    GCCGGCTACG ACCCCACCTA CGGCATGTTC CAGGGCATCC CCAGCAATGA CCCCATCAAC
    GTGCTGGTCC GGGTCTACGT GGTCCGGGCC ACGGACCTGC ACCCCGCAGA CATCAACGGC
    AAAGCTGACC CGTACATCGC CATCCGGCTA GGCAAGACTG ATATCCGTGA CAAGGAGAAC
    TACATCTCCA AGCAGCTCAA CCCCGTCTTT GGGAAGTCCT TTGACATCGA AGCCTGCTTC
    CCCATGGAAT CCATGCTGAC GGTGGCCGTG TACGACTGGG ATCTGGTAGG CACTGATGAC
    CTCATTGGGG AAACCAAGAT CGACCTGGAA AACCGCTTCT ACAGCAAGCA CCGCGCCACC
    TGCGGCATCG CCCAGACCTA CTCCGTACAC GGCTACAATA TCTGGCGGGA CCCTATGAAG
    CCCAGCCAGA TCCTGACCCG CCTCTGCAAG GAGGGCAAAG TGGACGGCCC CCACTTCGGG
    CCCCCCGGGA GAGTGAAGGT GTCCAACCGC ATCTTCACAG GGCCCTCTGA GATCGAGGAC
    GAGAATGGCC AGAGGAAGCC CACAGAAGAG CACGTGGCAC TGCTGGCCCT GAGACACTGG
    GAGGACATCC CCCGCGTGGG CTGTCGCCTG GTGCCCGAAC ATGTGGAGAC ACGGCCGCTG
    CTCAACCCCG ACAAGCCGGG CATCGAACAG GGCCGCCTGG AGCTGTGGGT GGACATGTTT
    CCCATGGACA TGCCGTCCCC CGGGACACCT CTGGACATCT CCCCTCGGAA GCCCAAGAAG
    TACGAGCTGA GGGTCATCGT GTGGAACACG GATGAGGTGG TCTTGGAAGA TGACGACTTC
    TTCACTGGGG AGAAGTCCAG CGACATTTTC GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG
    GACAAGCAGG ACACAGACGT CCACTACCAC TCCCTCACGG GCGAGGGCAA CTTCAACTGG
    CGCTACCTGT TCCCCTTCGA CTACCTGGCT GCCGAGGAGA AGATAGTCAT CTCCAAGAAG
    GAGTCCATGT TCTCCTGGGA CGAGACCGAA TACAAGATCC CCGCACGGCT CACCCTGCAG
    ATCTGGGACG CCGACCACTT CTCTGCCGAT GACTTCCTGG GGGCCATCGA GCTGGACCTG
    AATCGCTTCC CTCGGGGCGC GAAAACGGCC AAGCAGTGCA CCATGGAGAT GGCCACAGGG
    GAGGTGGATG TGCCCCTGGT TTCCATCTTC AAGCAGAAGC GTGTCAAAGG CTGGTGGCCC
    CTCCTAGCCC GCAACGAGAA TGACGAGTTT GAGCTCACGG GCAAAGTGGA AGCAGAGTTG
    CATCTACTCA CCGCCGAGGA GGCGGAAAAG AATCCCGTGG GCCTGGCCCG CAATGAACCT
    GATCCCCTAG AGAAACCCAA CCGGCCCGAC ACCGCCTTCG TCTGGTTCCT CAACCCCCTC
    AAGTCCATCA AGTACCTCAT CTGCACGCGG TACAAGTGGC TCATCATCAA GATCGTGTTG
    GCGCTGCTGG GACTGCTCAT GCTGGCGCTC TTCCTCTACA GCCTCCCCGG TTACATGGTC
    AAGAAGCTCT TGGGGGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91932
    Clone ID Related Accession (Same CDS sequence) XM_015473546.1 , XM_015473546.2
    Accession Version XM_015473546.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product otoferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473546.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT
    GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG
    ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG
    ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC
    GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC
    CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG
    CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG
    GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC
    GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA
    GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC
    ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC
    GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC
    TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC
    GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC
    GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG
    GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT
    GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG
    CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG
    GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC
    GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG
    GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC
    TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC
    GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA
    ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC
    GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG
    GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG
    GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC
    TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC
    CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA
    GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT
    CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG
    GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC
    GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT
    GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC
    AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGGTGC CGGAAGGGGA ATGGATTAGC
    AGCGGGTGGA GGGGCTGGGG GCTGGGCTAC TCTCAAGAGG TCATCTTCCT CTTGGAGGGA
    GCAGCATCCA CACCCCTGGG TCTGCCAGGC CTGAGTGGAC AAGATGCTCA GGGAAGAGCT
    AAAGAGAATA GGAAGGGGGA TCCCGCCCTG CCCATCCCCT TCTGTGCTGT GTTTCCCCAC
    TGCACAGCCC AGTGGGATGG CTGCGGTGGG GATGGGCCCC CTCTGACCAC AGCTTCTTCA
    GCTCCCTGCC CCTTCCTCAC CCCGAGGGCT CTGGCAGCTG ACCCTGAGCC CACCCCCCAG
    CTGCCAGGGA AGCGGGGCTT CGGCTCGGCA GGCTGGACAG TGCAGGCCAA GCTGGAGCTC
    TACCTGTGGC TGGGTCTAAG CAAGCAGCGC AAGGACTTCC TGTGTGGCCT GCCCTGCGGC
    TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG GGCCTACTCT CCTTCCCGCC CATCAGCCTG
    GTCTACACTA AGAAGCAGGC CTTCCAGCTC CGCGCCCACA TGTACCAGGC TCGCAGCCTC
    TTTGCTGCTG ACAGCACCGG CCTCTCAGAC CCCTTTGCCC GTGTCTTCTT CATCAACCAG
    AGTCAGTGCA CAGAGGTACT GAATGAGACC CTGTGCCCCA CCTGGGACCA GATGCTGGTG
    TTCGACAACC TGGAGCTCTA CGGTGAAGCT CATGAGCTGC GGGACGATCC CCCCATCATT
    GTTATCGAAA TCTACGACCA GGACACCATG GGCAAAGCCG ACTTCATGGG CCGGACCTTT
    GCCAAGCCGC TGGTGAAGAT GGCCGACGAG GCGTACTGCC CACCCCGCTT CCCGCCCCAG
    CTGGAGTACT ATCAGATCTA CCGGGGCAAT GCCACGGCCG GGGACCTGCT GGCTGCCTTC
    GAGCTGCTGC AGATTGGACC AGCAGGGAAG GCCGACCTGC CGCCGATCAA CGGCCCAGTG
    GACATGGACC GAGGTCCCAT CATGCCTGTG CCTGTGGGCA TCCGACCCGT GCTTAGCAAG
    TACCGGGTGG AGGTGCTGTT CTGGGGCCTG CGAGACCTGA AGCGGGTAAA CCTGGCCCAG
    GTGGACAGGC CGCGGGTGGA CATCGAGTGC GCGGGGAAGG GGGTGCAGTC CTCCCTGATC
    CACAATTACA AGAAGAACCC CAACTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGACCTC
    CCAGAGAACG AGCTCCTGCA CCCGCCCTTG AACATCCGCG TGGTGGACTG CCGGGCCTTC
    GGCCGCTACA CCCTGGTCGG CTCCCACGCT GTCAGCTCCC TGCGGCGCTT CATCTACCGA
    CCCCCGGACC GCTCAGCTCC CAACTGGAAC ACTTCGGGGG AGGTCGTGGT GAGCATGGAG
    CCGGAGGTGC CCATCAAGAA GCTGGAGACC ATGGTGAAGC TGGACGCGAC TTCCGATGCC
    GTCATCAAGG TGGATGTGGC TGAGGAAGAG AAGGAGAGGA AGAAGAAGAA GAAGAAGGGC
    AGCTCAGATG AGCCGGAGGA GGAGGAGCCA GATGAGAGTA TGTTGGACTG GTGGTCCAAG
    TACTTTGCCT CCATTGACAC CATGAAAGAG CAAATTCGAC AACAAGAAAT CTCAGGAATT
    GACCTGGAGG AGAAGGAGGA GATGGACAAC ACTGAAGGCC TGAAGGGGCC GATGAAGGGC
    AAGGAGAAGG CAAGGGCTGC AAAGGAGGAG AAGAAGAAAA GAACCCAGAG CCTAGGCCCT
    GGCCAGGGGT CGGAGGCCCC CGAGAAGAGG AAACCCAAGA TTGATGAGCT CAAGGTGTAC
    CCCAAGGAGC TGGAGTCAGA GTTCGACAAC TTCGAGGACT GGCTGCACAC GTTCAACCTG
    CTTCGGGGGA AGACGGGGGA CGACGAGGAT GGCGGGACCG AGGAGGAGCG CATTGTGGGC
    CGGTTCAAGG GCTCCCTCTG CGTGTACAAA GTGCCACTCC CAGAGGACGT GTCCCGGGAA
    GCCGGCTACG ACCCCACCTA CGGCATGTTC CAGGGCATCC CCAGCAATGA CCCCATCAAC
    GTGCTGGTCC GGGTCTACGT GGTCCGGGCC ACGGACCTGC ACCCCGCAGA CATCAACGGC
    AAAGCTGACC CGTACATCGC CATCCGGCTA GGCAAGACTG ATATCCGTGA CAAGGAGAAC
    TACATCTCCA AGCAGCTCAA CCCCGTCTTT GGGAAGTCCT TTGACATCGA AGCCTGCTTC
    CCCATGGAAT CCATGCTGAC GGTGGCCGTG TACGACTGGG ATCTGGTAGG CACTGATGAC
    CTCATTGGGG AAACCAAGAT CGACCTGGAA AACCGCTTCT ACAGCAAGCA CCGCGCCACC
    TGCGGCATCG CCCAGACCTA CTCCGTACAC GGCTACAATA TCTGGCGGGA CCCTATGAAG
    CCCAGCCAGA TCCTGACCCG CCTCTGCAAG GAGGGCAAAG TGGACGGCCC CCACTTCGGG
    CCCCCCGGGA GAGTGAAGGT GTCCAACCGC ATCTTCACAG GGCCCTCTGA GATCGAGGAC
    GAGAATGGCC AGAGGAAGCC CACAGAAGAG CACGTGGCAC TGCTGGCCCT GAGACACTGG
    GAGGACATCC CCCGCGTGGG CTGTCGCCTG GTGCCCGAAC ATGTGGAGAC ACGGCCGCTG
    CTCAACCCCG ACAAGCCGGG CATCGAACAG GGCCGCCTGG AGCTGTGGGT GGACATGTTT
    CCCATGGACA TGCCGTCCCC CGGGACACCT CTGGACATCT CCCCTCGGAA GCCCAAGAAG
    TACGAGCTGA GGGTCATCGT GTGGAACACG GATGAGGTGG TCTTGGAAGA TGACGACTTC
    TTCACTGGGG AGAAGTCCAG CGACATTTTC GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG
    GACAAGCAGG ACACAGACGT CCACTACCAC TCCCTCACGG GCGAGGGCAA CTTCAACTGG
    CGCTACCTGT TCCCCTTCGA CTACCTGGCT GCCGAGGAGA AGATAGTCAT CTCCAAGAAG
    GAGTCCATGT TCTCCTGGGA CGAGACCGAA TACAAGATCC CCGCACGGCT CACCCTGCAG
    ATCTGGGACG CCGACCACTT CTCTGCCGAT GACTTCCTGG GGGCCATCGA GCTGGACCTG
    AATCGCTTCC CTCGGGGCGC GAAAACGGCC AAGCAGTGCA CCATGGAGAT GGCCACAGGG
    GAGGTGGATG TGCCCCTGGT TTCCATCTTC AAGCAGAAGC GTGTCAAAGG CTGGTGGCCC
    CTCCTAGCCC GCAACGAGAA TGACGAGTTT GAGCTCACGG GCAAAGTGGA AGCAGAGTTG
    CATCTACTCA CCGCCGAGGA GGCGGAAAAG AATCCCGTGG GCCTGGCCCG CAATGAACCT
    GATCCCCTAG AGAAACCCAA CCGGCCCGAC ACCGCCTTCG TCTGGTTCCT CAACCCCCTC
    AAGTCCATCA AGTACCTCAT CTGCACGCGG TACAAGTGGC TCATCATCAA GATCGTGTTG
    GCGCTGCTGG GACTGCTCAT GCTGGCGCTC TTCCTCTACA GCCTCCCCGG TTACATGGTC
    AAGAAGCTCT TGGGGGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329032.1
    CDS181..6441
    Translation

    Target ORF information:

    RefSeq Version XM_015473546.2
    Organism Bos taurus(cattle)
    Definition Bos taurus otoferlin (OTOF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473546.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCCTGC TCGTCCACCT CAAGACAGTC TCCGAGCTGC GGGGCAAGGG TGACCGGATC 
    GCCAAAGTGA CTTTCCGAGG GCAGTCCTTC TACTCCCGGG TCCTGGAGAG CTGTGAGGAT
    GTGGCTGACT TCGATGAGAC CTTCCGGTGG CCGGTGGCCA GCAGCATCGA CAGCAATGAG
    ATGCTCGAGA TTCAGATCTT CAACTACAGC AAAGTCTTTA GCAACAAGCT GATCGGGACC
    TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAACCACG TGGAGGTGAC CGACACGCTG
    ATGGATGACA ACAATGCAAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CAGTGGGCTC CTGGGATGAC GGGGACTTTC TGGGAGAGGA GTCCCTTCAC
    GAGGAAGAGA AGGACAGCCA GGAGACGGAT GGGCTGCTAC CCGGCTCCCG TCCCAGCTCC
    CGGCCATCAG GCGAGAAGAG TTTCCGGAGA GCCGGGAGAA GCGTGTTCTC CGCCATGAAG
    CTCGGCAAGA ACCGGCCTCA CAAGGAGGAA CCCCAAAGAC AAGATGAACC AGCTGTTCTG
    GAGATGGAGG ACCTTGACCG CCTGGCCATT CGGCTGGGGG ATGGGCTGGA CCCTGACTCC
    GTGTCGCTAG CATCCGTCAC AGCCCTCACC ACCAATGTCT CCAACAAGCG GTCTAAGCCA
    GACATCAAGA TGGAGCCAAG TGCCGGGCGG CCAATGGACT ACCAGGTCAG CGTCACAGTC
    ATCGAGGCTC GGCAGCTGGT GGGCTTGAAC ATGGACCCCG TGGTGTGTGT GGAGGTGGGC
    GATGAGAAGA AGTACACCTC CATGAAGGAG TCCACTAACT GCCCCTACTA TAATGAGTAC
    TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG
    GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGGTCCTT CAAAATGGAC
    GTGGGAACCG TGTACTCCCA GCCAGAGCAC CAGTTCCACC ACAAGTGGGC CGTCCTGTCC
    GACCCTGACG ACATCTCCGC GGGACTGAAG GGCTACGTGA AGTGCGATGT CGCTGTGGTG
    GGCAAGGGCG ACAACATTAA GACTCCCCAC AAGGCCAATG AGACGGATGA GGATGACATT
    GAGGGGAACT TGCTGCTCCC CGAAGGGGTG CCCCCTGAAC GCCAGTGGGC CCGGTTCTAC
    GTGAAGATCT ACCGAGCAGA GGGGCTGCCC CGCATGAACA CGAGCCTCAT GGCCAATGTG
    AAGAAGGCTT TCACTGGCGA GAACAAGGAT CTGGTCGACC CCTATGTGCA AGTCTTCTTT
    GCCGGCCAGA AGGGCAAGAC TTCAGTGCAG AAAAGCAGCT ACGAGCCACT GTGGAATGAG
    CAGGTGGTCT TCACAGACCT GTTTCCCCCA CTCTGCAAGC GCATGAAGGT GCAAATCCGG
    GACTCGGACA AGGTCAATGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT
    TCCAACGATG GAGACAAAGG CTTCCTGCCC ACGCTGGGCC CTGCCTGGGT GAACATGTAC
    GGCTCCACAC GCAATTACAC GCTGCTAGAC GAGCATCAGG ACCTGAACGA GGGCCTGGGG
    GAGGGTGTGT CCTTCCGTGC CCGCCTCATG CTGGGCCTGG CGGTGGAGAT CCTGGACACC
    TCCAACCCTG AGATCACCAG CTCCACTGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCC
    GAGAGCTGCA CGGGGAAAAT AGAAGAATTC TTTCTATTTG GAGCCTTCCT GGAGGCCTCA
    ATGATCGACC GGAAAAATGG AGACAAGCCC ATCACCTTTG AGGTCACCGT AGGCAACTAC
    GGGAACGAAG TCGATGGGCT GTCACGACCC CATCGGCCTC GGCCTCGGAA GGAGCCTGGG
    GATGAGGAGG AAGTCGATCT GATCCAGAAC TTGAGTGATG AAGAGACTGA CGAGGGTGGG
    GGCCTGGCCT CGGTCTCCTC CACGCCACCC ATGCGGCCCC AGATCACTGA CAGGAACTAC
    TTCCATCTGC CCTACCTGGA GCACAAACCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC
    CAACGCCGTC GCCTGTACAA TGCCAACATC ATGGACCACA TCGCAGACAA GCTGGAAGAA
    GGCCTGAATG ATGTGCAGGA GATGATCAAA ACGGAGAAGT CGTACCCTGA GCGTCGACTT
    CGGGGCGTCC TAGAGGAGCT GAGCTCTGGT TGCTACCGCT TCCTCTCCCT CGCGGACAAG
    GACCAGGGCC ACTCATCCCG CACCAGGCTG GACCGGGAGC GTCTCAAGTC CTGCATGCGG
    GAGCTGGAAA GCATGGGGCA GCAGGCCAAG ACCCTGCGGG CACAAGTGAA GCGGCACACG
    GTGCGCGACA AGCTGAGGCT CTGTCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCAGAC
    GAGCCCCAGC ACAGCATCCC TGACGTCTTC ATCTGGATGA TGAGCAACAA CAAGCGCATT
    GCCTACACCC GGGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAACTGGGC
    AAGGACTGTG CCAAGGTCAA GACACTCTTC CTCAAGGTGC CGGAAGGGGA ATGGATTAGC
    AGCGGGTGGA GGGGCTGGGG GCTGGGCTAC TCTCAAGAGG TCATCTTCCT CTTGGAGGGA
    GCAGCATCCA CACCCCTGGG TCTGCCAGGC CTGAGTGGAC AAGATGCTCA GGGAAGAGCT
    AAAGAGAATA GGAAGGGGGA TCCCGCCCTG CCCATCCCCT TCTGTGCTGT GTTTCCCCAC
    TGCACAGCCC AGTGGGATGG CTGCGGTGGG GATGGGCCCC CTCTGACCAC AGCTTCTTCA
    GCTCCCTGCC CCTTCCTCAC CCCGAGGGCT CTGGCAGCTG ACCCTGAGCC CACCCCCCAG
    CTGCCAGGGA AGCGGGGCTT CGGCTCGGCA GGCTGGACAG TGCAGGCCAA GCTGGAGCTC
    TACCTGTGGC TGGGTCTAAG CAAGCAGCGC AAGGACTTCC TGTGTGGCCT GCCCTGCGGC
    TTCGAGGAGG TCAAGGCAGC CCAGGGCCTG GGCCTACTCT CCTTCCCGCC CATCAGCCTG
    GTCTACACTA AGAAGCAGGC CTTCCAGCTC CGCGCCCACA TGTACCAGGC TCGCAGCCTC
    TTTGCTGCTG ACAGCACCGG CCTCTCAGAC CCCTTTGCCC GTGTCTTCTT CATCAACCAG
    AGTCAGTGCA CAGAGGTACT GAATGAGACC CTGTGCCCCA CCTGGGACCA GATGCTGGTG
    TTCGACAACC TGGAGCTCTA CGGTGAAGCT CATGAGCTGC GGGACGATCC CCCCATCATT
    GTTATCGAAA TCTACGACCA GGACACCATG GGCAAAGCCG ACTTCATGGG CCGGACCTTT
    GCCAAGCCGC TGGTGAAGAT GGCCGACGAG GCGTACTGCC CACCCCGCTT CCCGCCCCAG
    CTGGAGTACT ATCAGATCTA CCGGGGCAAT GCCACGGCCG GGGACCTGCT GGCTGCCTTC
    GAGCTGCTGC AGATTGGACC AGCAGGGAAG GCCGACCTGC CGCCGATCAA CGGCCCAGTG
    GACATGGACC GAGGTCCCAT CATGCCTGTG CCTGTGGGCA TCCGACCCGT GCTTAGCAAG
    TACCGGGTGG AGGTGCTGTT CTGGGGCCTG CGAGACCTGA AGCGGGTAAA CCTGGCCCAG
    GTGGACAGGC CGCGGGTGGA CATCGAGTGC GCGGGGAAGG GGGTGCAGTC CTCCCTGATC
    CACAATTACA AGAAGAACCC CAACTTCAGC ACCCTGGTCA AGTGGTTTGA AGTGGACCTC
    CCAGAGAACG AGCTCCTGCA CCCGCCCTTG AACATCCGCG TGGTGGACTG CCGGGCCTTC
    GGCCGCTACA CCCTGGTCGG CTCCCACGCT GTCAGCTCCC TGCGGCGCTT CATCTACCGA
    CCCCCGGACC GCTCAGCTCC CAACTGGAAC ACTTCGGGGG AGGTCGTGGT GAGCATGGAG
    CCGGAGGTGC CCATCAAGAA GCTGGAGACC ATGGTGAAGC TGGACGCGAC TTCCGATGCC
    GTCATCAAGG TGGATGTGGC TGAGGAAGAG AAGGAGAGGA AGAAGAAGAA GAAGAAGGGC
    AGCTCAGATG AGCCGGAGGA GGAGGAGCCA GATGAGAGTA TGTTGGACTG GTGGTCCAAG
    TACTTTGCCT CCATTGACAC CATGAAAGAG CAAATTCGAC AACAAGAAAT CTCAGGAATT
    GACCTGGAGG AGAAGGAGGA GATGGACAAC ACTGAAGGCC TGAAGGGGCC GATGAAGGGC
    AAGGAGAAGG CAAGGGCTGC AAAGGAGGAG AAGAAGAAAA GAACCCAGAG CCTAGGCCCT
    GGCCAGGGGT CGGAGGCCCC CGAGAAGAGG AAACCCAAGA TTGATGAGCT CAAGGTGTAC
    CCCAAGGAGC TGGAGTCAGA GTTCGACAAC TTCGAGGACT GGCTGCACAC GTTCAACCTG
    CTTCGGGGGA AGACGGGGGA CGACGAGGAT GGCGGGACCG AGGAGGAGCG CATTGTGGGC
    CGGTTCAAGG GCTCCCTCTG CGTGTACAAA GTGCCACTCC CAGAGGACGT GTCCCGGGAA
    GCCGGCTACG ACCCCACCTA CGGCATGTTC CAGGGCATCC CCAGCAATGA CCCCATCAAC
    GTGCTGGTCC GGGTCTACGT GGTCCGGGCC ACGGACCTGC ACCCCGCAGA CATCAACGGC
    AAAGCTGACC CGTACATCGC CATCCGGCTA GGCAAGACTG ATATCCGTGA CAAGGAGAAC
    TACATCTCCA AGCAGCTCAA CCCCGTCTTT GGGAAGTCCT TTGACATCGA AGCCTGCTTC
    CCCATGGAAT CCATGCTGAC GGTGGCCGTG TACGACTGGG ATCTGGTAGG CACTGATGAC
    CTCATTGGGG AAACCAAGAT CGACCTGGAA AACCGCTTCT ACAGCAAGCA CCGCGCCACC
    TGCGGCATCG CCCAGACCTA CTCCGTACAC GGCTACAATA TCTGGCGGGA CCCTATGAAG
    CCCAGCCAGA TCCTGACCCG CCTCTGCAAG GAGGGCAAAG TGGACGGCCC CCACTTCGGG
    CCCCCCGGGA GAGTGAAGGT GTCCAACCGC ATCTTCACAG GGCCCTCTGA GATCGAGGAC
    GAGAATGGCC AGAGGAAGCC CACAGAAGAG CACGTGGCAC TGCTGGCCCT GAGACACTGG
    GAGGACATCC CCCGCGTGGG CTGTCGCCTG GTGCCCGAAC ATGTGGAGAC ACGGCCGCTG
    CTCAACCCCG ACAAGCCGGG CATCGAACAG GGCCGCCTGG AGCTGTGGGT GGACATGTTT
    CCCATGGACA TGCCGTCCCC CGGGACACCT CTGGACATCT CCCCTCGGAA GCCCAAGAAG
    TACGAGCTGA GGGTCATCGT GTGGAACACG GATGAGGTGG TCTTGGAAGA TGACGACTTC
    TTCACTGGGG AGAAGTCCAG CGACATTTTC GTGCGGGGGT GGCTGAAGGG CCAGCAGGAG
    GACAAGCAGG ACACAGACGT CCACTACCAC TCCCTCACGG GCGAGGGCAA CTTCAACTGG
    CGCTACCTGT TCCCCTTCGA CTACCTGGCT GCCGAGGAGA AGATAGTCAT CTCCAAGAAG
    GAGTCCATGT TCTCCTGGGA CGAGACCGAA TACAAGATCC CCGCACGGCT CACCCTGCAG
    ATCTGGGACG CCGACCACTT CTCTGCCGAT GACTTCCTGG GGGCCATCGA GCTGGACCTG
    AATCGCTTCC CTCGGGGCGC GAAAACGGCC AAGCAGTGCA CCATGGAGAT GGCCACAGGG
    GAGGTGGATG TGCCCCTGGT TTCCATCTTC AAGCAGAAGC GTGTCAAAGG CTGGTGGCCC
    CTCCTAGCCC GCAACGAGAA TGACGAGTTT GAGCTCACGG GCAAAGTGGA AGCAGAGTTG
    CATCTACTCA CCGCCGAGGA GGCGGAAAAG AATCCCGTGG GCCTGGCCCG CAATGAACCT
    GATCCCCTAG AGAAACCCAA CCGGCCCGAC ACCGCCTTCG TCTGGTTCCT CAACCCCCTC
    AAGTCCATCA AGTACCTCAT CTGCACGCGG TACAAGTGGC TCATCATCAA GATCGTGTTG
    GCGCTGCTGG GACTGCTCAT GCTGGCGCTC TTCCTCTACA GCCTCCCCGG TTACATGGTC
    AAGAAGCTCT TGGGGGCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.