Otof cDNA ORF clone, Mus musculus(house mouse)

The following Otof gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Otof cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu71866 XM_011240802.2
Latest version!
Mus musculus otoferlin (Otof), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu83581 NM_001313767.1
Latest version!
Mus musculus otoferlin (Otof), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu35276 XM_006504208.4
Latest version!
Mus musculus otoferlin (Otof), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu35277 XM_006504209.4
Latest version!
Mus musculus otoferlin (Otof), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu81542 XM_017321186.1
Latest version!
Mus musculus otoferlin (Otof), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OMu16363 NM_001100395.1
Latest version!
Mus musculus otoferlin (Otof), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1462.30
$2089.00
OMu16389 NM_031875.2
Latest version!
Mus musculus otoferlin (Otof), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu23304 NM_001286421.1
Latest version!
Mus musculus otoferlin (Otof), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu16389 XM_017321185.1
Latest version!
Mus musculus otoferlin (Otof), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu35274 XM_006504206.3
Latest version!
Mus musculus otoferlin (Otof), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
View Old Accession Versions >>
OMu35276 XM_006504208.3 Mus musculus otoferlin (Otof), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OMu35277 XM_006504209.3 Mus musculus otoferlin (Otof), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu71866
    Clone ID Related Accession (Same CDS sequence) XM_011240802.2
    Accession Version XM_011240802.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product otoferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGCCC CTTTCTCTGA GACGTAGACT GCAAAAATGT
    CACCACTACC CGTCAGGACC TCCCGGAGAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011239104.1
    CDS933..4565
    Translation

    Target ORF information:

    RefSeq Version XM_011240802.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus otoferlin (Otof), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011240802.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGCCC CTTTCTCTGA GACGTAGACT GCAAAAATGT
    CACCACTACC CGTCAGGACC TCCCGGAGAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu83581
    Clone ID Related Accession (Same CDS sequence) NM_001313767.1
    Accession Version NM_001313767.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Mus musculus(house mouse)
    Product otoferlin isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC157761.6, AC175379.4 and AC242405.4. Transcript Variant: This variant (4) uses an alternate in-frame splice junction in the 3' end and lacks the penultimate exon compared to variant 2. It encodes isoform 4, which lacks an internal segment and has a shorter and distinct C-terminus compared to isoform 2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGGAC CTCCCGGAGA ATGAGCTCCT GCACCCACCC
    TTGAACATCC GAGTGGTAGA TTGCCGGGCC TTTGGACGAT ACACCCTGGT GGGTTCCCAC
    GCAGTCAGCT CACTGAGGCG CTTCATCTAC CGACCTCCAG ACCGCTCAGC CCCCAACTGG
    AACACCACAG GGGAGGTTGT AGTAAGCATG GAGCCTGAGG AGCCAGTTAA GAAGCTGGAG
    ACCATGGTGA AACTGGATGC GACTTCTGAT GCTGTGGTCA AGGTGGATGT GGCTGAAGAT
    GAGAAGGAAA GGAAGAAGAA GAAAAAGAAA GGCCCGTCAG AGGAGCCAGA GGAGGAAGAG
    CCCGATGAGA GCATGCTGGA TTGGTGGTCC AAGTACTTCG CCTCCATCGA CACAATGAAG
    GAGCAACTTC GACAACATGA GACCTCTGGA ACTGACTTGG AAGAGAAGGA AGAGATGGAA
    AGCGCTGAGG GCCTGAAGGG ACCAATGAAG AGCAAGGAGA AGTCCAGAGC TGCAAAGGAG
    GAGAAAAAGA AGAAAAACCA GAGCCCTGGC CCTGGCCAGG GATCGGAGGC TCCTGAGAAG
    AAGAAAGCCA AGATCGATGA GCTTAAGGTG TACCCCAAGG AGCTGGAATC GGAGTTTGAC
    AGCTTTGAGG ACTGGCTGCA CACCTTCAAC CTGTTGAGGG GCAAGACGGG AGATGATGAG
    GATGGCTCCA CAGAGGAGGA GCGCATAGTA GGCCGATTCA AGGGCTCCCT CTGTGTGTAC
    AAAGTGCCAC TCCCAGAAGA TGTATCTCGA GAAGCTGGCT ATGATCCCAC CTATGGAATG
    TTCCAGGGCA TCCCAAGCAA TGACCCCATC AATGTGCTGG TCCGAATCTA TGTGGTCCGG
    GCCACAGACC TGCACCCGGC CGACATCAAT GGCAAAGCTG ACCCCTATAT TGCCATCAAG
    TTAGGCAAGA CCGACATCCG AGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTG
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AGTCCATGTT GACAGTGGCC
    GTGTACGACT GGGATCTGGT GGGCACTGAT GACCTCATCG GAGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCATCGCGCC ACCTGCGGCA TCGCACAGAC CTATTCCATA
    CATGGCTACA ATATCTGGAG GGACCCCATG AAGCCCAGCC AGATCCTGAC ACGCCTCTGT
    AAAGAGGGCA AAGTGGACGG CCCCCACTTT GGTCCCCATG GGAGAGTGAG GGTTGCCAAC
    CGTGTCTTCA CGGGGCCTTC AGAAATAGAG GATGAGAATG GTCAGAGGAA GCCCACAGAT
    GAGCACGTGG CACTGTCTGC TCTGAGACAC TGGGAGGACA TCCCCCGGGT GGGCTGCCGC
    CTTGTGCCGG AACACGTGGA GACCAGGCCG CTGCTCAACC CTGACAAGCC AGGCATTGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACA
    CCTCTGGATA TATCCCCCAG GAAACCCAAG AAGTACGAGC TGCGGGTCAT CGTGTGGAAC
    ACAGACGAGG TGGTCCTGGA AGACGATGAT TTCTTCACGG GAGAGAAGTC CAGTGACATT
    TTTGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAAC AGGACACAGA TGTCCACTAT
    CACTCCCTCA CGGGGGAGGG CAACTTCAAC TGGAGATACC TCTTCCCCTT CGACTACCTA
    GCGGCCGAAG AGAAGATCGT TATGTCCAAA AAGGAGTCTA TGTTCTCCTG GGATGAGACG
    GAGTACAAGA TCCCTGCGCG GCTCACCCTG CAGATCTGGG ACGCTGACCA CTTCTCGGCT
    GACGACTTCC TGGGGGCTAT CGAGCTGGAC CTGAACCGGT TCCCGAGGGG CGCTAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTACCCCT GGTTTCCATC
    TTTAAACAGA AACGTGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAATGATGAG
    TTTGAGCTCA CAGGCAAAGT GGAGGCGGAG CTACACCTAC TCACGGCAGA GGAGGCAGAG
    AAGAACCCTG TGGGCCTGGC TCGCAATGAA CCTGATCCCC TAGAAAAACC CAACCGGCCT
    GACACGGCAT TCGTCTGGTT CCTGAACCCA CTCAAATCTA TCAAGTACCT CATCTGCACC
    CGGTACAAGT GGCTGATCAT CAAGATCGTG CTGGCGCTGC TGGGGCTGCT CATGCTGGCC
    CTCTTCCTTT ACAGCCTCCC AGGCTACATG GTCAAGAAGC TCCTAGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001300696.1
    CDS135..6068
    Translation

    Target ORF information:

    RefSeq Version NM_001313767.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus otoferlin (Otof), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001313767.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGGAC CTCCCGGAGA ATGAGCTCCT GCACCCACCC
    TTGAACATCC GAGTGGTAGA TTGCCGGGCC TTTGGACGAT ACACCCTGGT GGGTTCCCAC
    GCAGTCAGCT CACTGAGGCG CTTCATCTAC CGACCTCCAG ACCGCTCAGC CCCCAACTGG
    AACACCACAG GGGAGGTTGT AGTAAGCATG GAGCCTGAGG AGCCAGTTAA GAAGCTGGAG
    ACCATGGTGA AACTGGATGC GACTTCTGAT GCTGTGGTCA AGGTGGATGT GGCTGAAGAT
    GAGAAGGAAA GGAAGAAGAA GAAAAAGAAA GGCCCGTCAG AGGAGCCAGA GGAGGAAGAG
    CCCGATGAGA GCATGCTGGA TTGGTGGTCC AAGTACTTCG CCTCCATCGA CACAATGAAG
    GAGCAACTTC GACAACATGA GACCTCTGGA ACTGACTTGG AAGAGAAGGA AGAGATGGAA
    AGCGCTGAGG GCCTGAAGGG ACCAATGAAG AGCAAGGAGA AGTCCAGAGC TGCAAAGGAG
    GAGAAAAAGA AGAAAAACCA GAGCCCTGGC CCTGGCCAGG GATCGGAGGC TCCTGAGAAG
    AAGAAAGCCA AGATCGATGA GCTTAAGGTG TACCCCAAGG AGCTGGAATC GGAGTTTGAC
    AGCTTTGAGG ACTGGCTGCA CACCTTCAAC CTGTTGAGGG GCAAGACGGG AGATGATGAG
    GATGGCTCCA CAGAGGAGGA GCGCATAGTA GGCCGATTCA AGGGCTCCCT CTGTGTGTAC
    AAAGTGCCAC TCCCAGAAGA TGTATCTCGA GAAGCTGGCT ATGATCCCAC CTATGGAATG
    TTCCAGGGCA TCCCAAGCAA TGACCCCATC AATGTGCTGG TCCGAATCTA TGTGGTCCGG
    GCCACAGACC TGCACCCGGC CGACATCAAT GGCAAAGCTG ACCCCTATAT TGCCATCAAG
    TTAGGCAAGA CCGACATCCG AGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTG
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AGTCCATGTT GACAGTGGCC
    GTGTACGACT GGGATCTGGT GGGCACTGAT GACCTCATCG GAGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCATCGCGCC ACCTGCGGCA TCGCACAGAC CTATTCCATA
    CATGGCTACA ATATCTGGAG GGACCCCATG AAGCCCAGCC AGATCCTGAC ACGCCTCTGT
    AAAGAGGGCA AAGTGGACGG CCCCCACTTT GGTCCCCATG GGAGAGTGAG GGTTGCCAAC
    CGTGTCTTCA CGGGGCCTTC AGAAATAGAG GATGAGAATG GTCAGAGGAA GCCCACAGAT
    GAGCACGTGG CACTGTCTGC TCTGAGACAC TGGGAGGACA TCCCCCGGGT GGGCTGCCGC
    CTTGTGCCGG AACACGTGGA GACCAGGCCG CTGCTCAACC CTGACAAGCC AGGCATTGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACA
    CCTCTGGATA TATCCCCCAG GAAACCCAAG AAGTACGAGC TGCGGGTCAT CGTGTGGAAC
    ACAGACGAGG TGGTCCTGGA AGACGATGAT TTCTTCACGG GAGAGAAGTC CAGTGACATT
    TTTGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAAC AGGACACAGA TGTCCACTAT
    CACTCCCTCA CGGGGGAGGG CAACTTCAAC TGGAGATACC TCTTCCCCTT CGACTACCTA
    GCGGCCGAAG AGAAGATCGT TATGTCCAAA AAGGAGTCTA TGTTCTCCTG GGATGAGACG
    GAGTACAAGA TCCCTGCGCG GCTCACCCTG CAGATCTGGG ACGCTGACCA CTTCTCGGCT
    GACGACTTCC TGGGGGCTAT CGAGCTGGAC CTGAACCGGT TCCCGAGGGG CGCTAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTACCCCT GGTTTCCATC
    TTTAAACAGA AACGTGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAATGATGAG
    TTTGAGCTCA CAGGCAAAGT GGAGGCGGAG CTACACCTAC TCACGGCAGA GGAGGCAGAG
    AAGAACCCTG TGGGCCTGGC TCGCAATGAA CCTGATCCCC TAGAAAAACC CAACCGGCCT
    GACACGGCAT TCGTCTGGTT CCTGAACCCA CTCAAATCTA TCAAGTACCT CATCTGCACC
    CGGTACAAGT GGCTGATCAT CAAGATCGTG CTGGCGCTGC TGGGGCTGCT CATGCTGGCC
    CTCTTCCTTT ACAGCCTCCC AGGCTACATG GTCAAGAAGC TCCTAGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35276
    Clone ID Related Accession (Same CDS sequence) XM_006504208.3 , XM_006504208.4
    Accession Version XM_006504208.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5820bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product otoferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504208.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGGAC CTCCCGGAGA ATGAGCTCCT GCACCCACCC
    TTGAACATCC GAGTGGTAGA TTGCCGGGCC TTTGGACGAT ACACCCTGGT GGGTTCCCAC
    GCAGTCAGCT CACTGAGGCG CTTCATCTAC CGACCTCCAG ACCGCTCAGC CCCCAACTGG
    AACACCACAG TCAGGCTGCT CCGGGGCTGC CACAGGCTGC GCAATGGGGG CCCCTCTTCT
    CGCCCCACAG GGGAGGTTGT AGTAAGCATG GAGCCTGAGG AGCCAGTTAA GAAGCTGGAG
    ACCATGGTGA AACTGGATGC GACTTCTGAT GCTGTGGTCA AGGTGGATGT GGCTGAAGAT
    GAGAAGGAAA GGAAGAAGAA GAAAAAGAAA GGCCCGTCAG AGGAGCCAGA GGAGGAAGAG
    CCCGATGAGA GCATGCTGGA TTGGTGGTCC AAGTACTTCG CCTCCATCGA CACAATGAAG
    GAGCAACTTC GACAACATGA GACCTCTGGA ACTGACTTGG AAGAGAAGGA AGAGATGGAA
    AGCGCTGAGG GCCTGAAGGG ACCAATGAAG AGCAAGGAGA AGTCCAGAGC TGCAAAGGAG
    GAGAAAAAGA AGAAAAACCA GAGCCCTGGC CCTGGCCAGG GATCGGAGGC TCCTGAGAAG
    AAGAAAGCCA AGATCGATGA GCTTAAGGTG TACCCCAAGG AGCTGGAATC GGAGTTTGAC
    AGCTTTGAGG ACTGGCTGCA CACCTTCAAC CTGTTGAGGG GCAAGACGGG AGATGATGAG
    GATGGCTCCA CAGAGGAGGA GCGCATAGTA GGCCGATTCA AGGGCTCCCT CTGTGTGTAC
    AAAGTGCCAC TCCCAGAAGA TGTATCTCGA GAAGCTGGCT ATGATCCCAC CTATGGAATG
    TTCCAGGGCA TCCCAAGCAA TGACCCCATC AATGTGCTGG TCCGAATCTA TGTGGTCCGG
    GCCACAGACC TGCACCCGGC CGACATCAAT GGCAAAGCTG ACCCCTATAT TGCCATCAAG
    TTAGGCAAGA CCGACATCCG AGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTG
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AGTCCATGTT GACAGTGGCC
    GTGTACGACT GGGATCTGGT GGGCACTGAT GACCTCATCG GAGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCATCGCGCC ACCTGCGGCA TCGCACAGAC CTATTCCATA
    CATGGCTACA ATATCTGGAG GGACCCCATG AAGCCCAGCC AGATCCTGAC ACGCCTCTGT
    AAAGAGGGCA AAGTGGACGG CCCCCACTTT GGTCCCCATG GGAGAGTGAG GGTTGCCAAC
    CGTGTCTTCA CGGGGCCTTC AGAAATAGAG GATGAGAATG GTCAGAGGAA GCCCACAGAT
    GAGCACGTGG CACTGTCTGC TCTGAGACAC TGGGAGGACA TCCCCCGGGT GGGCTGCCGC
    CTTGTGCCGG AACACGTGGA GACCAGGCCG CTGCTCAACC CTGACAAGCC AGGCATTGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACA
    CCTCTGGATA TATCCCCCAG GAAACCCAAG AAGTACGAGC TGCGGGTCAT CGTGTGGAAC
    ACAGACGAGG TGGTCCTGGA AGACGATGAT TTCTTCACGG GAGAGAAGTC CAGTGACATT
    TTTGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAAC AGGACACAGA TGTCCACTAT
    CACTCCCTCA CGGGGGAGGG CAACTTCAAC TGGAGATACC TCTTCCCCTT CGACTACCTA
    GCGGCCGAAG AGAAGATCGT TATGTCCAAA AAGGAGTCTA TGTTCTCCTG GGATGAGACG
    GAGTACAAGA TCCCTGCGCG GCTCACCCTG CAGATCTGGG ACGCTGACCA CTTCTCGGCT
    GACGACTTCC TGGGGGCTAT CGAGCTGGAC CTGAACCGGT TCCCGAGGGG CGCTAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTACCCCT GGTTTCCATC
    TTTAAACAGA AACGTGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAATGATGAG
    TTTGAGCTCA CAGGCAAAGT GGAGGCGGAG CTACACCTAC TCACGGCAGA GGAGGCAGAG
    AAGAACCCTG TGGGCCTGGC TCGCAATGAA CCTGATCCCC TAGAAAAACC CAATTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006504271.1
    CDS907..6726
    Translation

    Target ORF information:

    RefSeq Version XM_006504208.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus otoferlin (Otof), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006504208.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGGAC CTCCCGGAGA ATGAGCTCCT GCACCCACCC
    TTGAACATCC GAGTGGTAGA TTGCCGGGCC TTTGGACGAT ACACCCTGGT GGGTTCCCAC
    GCAGTCAGCT CACTGAGGCG CTTCATCTAC CGACCTCCAG ACCGCTCAGC CCCCAACTGG
    AACACCACAG TCAGGCTGCT CCGGGGCTGC CACAGGCTGC GCAATGGGGG CCCCTCTTCT
    CGCCCCACAG GGGAGGTTGT AGTAAGCATG GAGCCTGAGG AGCCAGTTAA GAAGCTGGAG
    ACCATGGTGA AACTGGATGC GACTTCTGAT GCTGTGGTCA AGGTGGATGT GGCTGAAGAT
    GAGAAGGAAA GGAAGAAGAA GAAAAAGAAA GGCCCGTCAG AGGAGCCAGA GGAGGAAGAG
    CCCGATGAGA GCATGCTGGA TTGGTGGTCC AAGTACTTCG CCTCCATCGA CACAATGAAG
    GAGCAACTTC GACAACATGA GACCTCTGGA ACTGACTTGG AAGAGAAGGA AGAGATGGAA
    AGCGCTGAGG GCCTGAAGGG ACCAATGAAG AGCAAGGAGA AGTCCAGAGC TGCAAAGGAG
    GAGAAAAAGA AGAAAAACCA GAGCCCTGGC CCTGGCCAGG GATCGGAGGC TCCTGAGAAG
    AAGAAAGCCA AGATCGATGA GCTTAAGGTG TACCCCAAGG AGCTGGAATC GGAGTTTGAC
    AGCTTTGAGG ACTGGCTGCA CACCTTCAAC CTGTTGAGGG GCAAGACGGG AGATGATGAG
    GATGGCTCCA CAGAGGAGGA GCGCATAGTA GGCCGATTCA AGGGCTCCCT CTGTGTGTAC
    AAAGTGCCAC TCCCAGAAGA TGTATCTCGA GAAGCTGGCT ATGATCCCAC CTATGGAATG
    TTCCAGGGCA TCCCAAGCAA TGACCCCATC AATGTGCTGG TCCGAATCTA TGTGGTCCGG
    GCCACAGACC TGCACCCGGC CGACATCAAT GGCAAAGCTG ACCCCTATAT TGCCATCAAG
    TTAGGCAAGA CCGACATCCG AGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTG
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AGTCCATGTT GACAGTGGCC
    GTGTACGACT GGGATCTGGT GGGCACTGAT GACCTCATCG GAGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCATCGCGCC ACCTGCGGCA TCGCACAGAC CTATTCCATA
    CATGGCTACA ATATCTGGAG GGACCCCATG AAGCCCAGCC AGATCCTGAC ACGCCTCTGT
    AAAGAGGGCA AAGTGGACGG CCCCCACTTT GGTCCCCATG GGAGAGTGAG GGTTGCCAAC
    CGTGTCTTCA CGGGGCCTTC AGAAATAGAG GATGAGAATG GTCAGAGGAA GCCCACAGAT
    GAGCACGTGG CACTGTCTGC TCTGAGACAC TGGGAGGACA TCCCCCGGGT GGGCTGCCGC
    CTTGTGCCGG AACACGTGGA GACCAGGCCG CTGCTCAACC CTGACAAGCC AGGCATTGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACA
    CCTCTGGATA TATCCCCCAG GAAACCCAAG AAGTACGAGC TGCGGGTCAT CGTGTGGAAC
    ACAGACGAGG TGGTCCTGGA AGACGATGAT TTCTTCACGG GAGAGAAGTC CAGTGACATT
    TTTGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAAC AGGACACAGA TGTCCACTAT
    CACTCCCTCA CGGGGGAGGG CAACTTCAAC TGGAGATACC TCTTCCCCTT CGACTACCTA
    GCGGCCGAAG AGAAGATCGT TATGTCCAAA AAGGAGTCTA TGTTCTCCTG GGATGAGACG
    GAGTACAAGA TCCCTGCGCG GCTCACCCTG CAGATCTGGG ACGCTGACCA CTTCTCGGCT
    GACGACTTCC TGGGGGCTAT CGAGCTGGAC CTGAACCGGT TCCCGAGGGG CGCTAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTACCCCT GGTTTCCATC
    TTTAAACAGA AACGTGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAATGATGAG
    TTTGAGCTCA CAGGCAAAGT GGAGGCGGAG CTACACCTAC TCACGGCAGA GGAGGCAGAG
    AAGAACCCTG TGGGCCTGGC TCGCAATGAA CCTGATCCCC TAGAAAAACC CAATTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35277
    Clone ID Related Accession (Same CDS sequence) XM_006504209.4 , XM_006504209.3
    Accession Version XM_006504209.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product otoferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504209.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAACTCG GCAAAACTCG GTCCCACAAA GAGGAGCCCC AAAGACAAGA TGAGCCAGCA 
    GTGCTGGAGA TGGAGGACCT GGACCACCTA GCCATTCAGC TGGGGGATGG GCTGGATCCT
    GACTCCGTGT CTCTAGCCTC GGTCACCGCT CTCACCAGCA ATGTCTCCAA CAAACGGTCT
    AAGCCAGATA TTAAGATGGA GCCCAGTGCT GGAAGGCCCA TGGATTACCA GGTCAGCATC
    ACAGTGATTG AGGCTCGGCA GCTGGTGGGC TTGAACATGG ACCCTGTGGT GTGTGTGGAG
    GTGGGTGATG ACAAGAAATA CACGTCAATG AAGGAGTCCA CAAACTGCCC TTACTACAAC
    GAGTACTTTG TCTTCGACTT CCATGTCTCT CCTGATGTCA TGTTTGACAA GATCATCAAG
    ATCTCGGTTA TCCATTCTAA GAACCTGCTT CGGAGCGGCA CCCTGGTGGG TTCCTTCAAA
    ATGGATGTGG GGACTGTGTA TTCCCAGCCT GAACACCAGT TCCATCACAA ATGGGCCATC
    CTGTCAGACC CCGATGACAT CTCTGCTGGG TTGAAGGGTT ATGTAAAGTG TGATGTCGCT
    GTGGTGGGCA AGGGAGACAA CATCAAGACA CCCCACAAGG CCAACGAGAC GGATGAGGAC
    GACATTGAAG GGAACTTGCT GCTCCCCGAG GGCGTGCCCC CCGAACGGCA GTGGGCACGG
    TTCTATGTGA AAATTTACCG AGCAGAGGGA CTGCCCCGGA TGAACACAAG CCTCATGGCC
    AACGTGAAGA AGGCGTTCAT CGGTGAGAAC AAGGACCTCG TCGACCCCTA TGTGCAAGTC
    TTCTTTGCTG GACAAAAGGG CAAAACATCA GTGCAGAAGA GCAGCTATGA GCCGCTATGG
    AATGAGCAGG TCGTCTTCAC AGACTTGTTC CCCCCACTCT GCAAACGCAT GAAGGTGCAG
    ATCCGGGACT CTGACAAGGT CAATGATGTG GCCATCGGCA CCCACTTCAT CGACCTGCGC
    AAGATTTCCA ACGATGGAGA CAAAGGCTTC CTGCCTACCC TCGGTCCAGC CTGGGTGAAC
    ATGTACGGCT CCACGCGCAA CTACACACTG CTGGACGAGC ACCAGGACTT GAATGAAGGC
    CTGGGGGAGG GTGTGTCCTT CCGGGCCCGC CTCATGTTGG GACTAGCTGT GGAGATCCTG
    GACACCTCCA ACCCAGAGCT CACCAGCTCC ACGGAGGTGC AGGTGGAGCA GGCCACGCCT
    GTCTCGGAGA GCTGCACAGG GAGAATGGAA GAATTTTTTC TATTTGGAGC CTTCTTGGAA
    GCCTCAATGA TTGACCGGAA AAATGGGGAC AAGCCAATTA CCTTTGAGGT GACCATAGGA
    AACTACGGCA ATGAAGTCGA TGGTATGTCC CGGCCCCTGA GGCCTCGGCC CCGGAAAGAG
    CCTGGGGATG AAGAAGAGGT AGACCTGATT CAGAACTCCA GTGACGATGA AGGTGACGAA
    GCCGGGGACC TGGCCTCGGT GTCCTCCACC CCACCTATGC GGCCCCAGAT CACGGACAGG
    AACTATTTCC ACCTGCCCTA CCTGGAGCGC AAGCCCTGCA TCTATATCAA GAGCTGGTGG
    CCTGACCAGA GGCGGCGCCT CTACAATGCC AACATCATGG ATCACATTGC TGACAAGCTG
    GAAGAAGGCC TGAATGATGT ACAGGAGATG ATCAAAACGG AGAAGTCCTA CCCGGAGCGC
    CGCCTGCGGG GTGTGCTAGA GGAACTCAGC TGTGGCTGCC ACCGCTTCCT CTCCCTCTCG
    GACAAGGACC AGGGCCGCTC GTCCCGCACC AGGCTGGATC GAGAGCGTCT TAAGTCCTGT
    ATGAGGGAGT TGGAGAGCAT GGGACAGCAG GCCAAGAGCC TGAGGGCTCA GGTGAAGCGG
    CACACTGTTC GGGACAAGCT GAGGTCATGC CAGAACTTTC TGCAGAAGCT ACGCTTCCTG
    GCGGATGAGC CCCAGCACAG CATTCCTGAT GTGTTCATTT GGATGATGAG CAACAACAAA
    CGTATCGCCT ATGCCCGCGT GCCTTCCAAA GACCTGCTCT TCTCCATCGT GGAGGAGGAA
    CTGGGCAAGG ACTGCGCCAA AGTCAAGACC CTCTTCCTGA AGCTGCCAGG GAAGAGGGGC
    TTCGGCTCGG CAGGCTGGAC AGTACAGGCC AAGCTGGAGC TCTACCTGTG GCTGGGCCTC
    AGCAAGCAGC GAAAGGACTT CCTGTGTGGT CTGCCCTGTG GCTTCGAGGA GGTCAAGGCA
    GCCCAAGGCC TGGGCCTGCA TTCCTTTCCG CCCATCAGCC TAGTCTACAC CAAGAAGCAA
    GCCTTCCAGC TCCGAGCACA CATGTATCAG GCCCGAAGCC TCTTTGCTGC TGACAGCAGT
    GGGCTCTCTG ATCCCTTTGC CCGTGTCTTC TTCATCAACC AGAGCCAATG CACTGAGGTT
    CTAAACGAGA CACTGTGTCC CACCTGGGAC CAGATGCTGG TATTTGACAA CCTGGAGCTG
    TACGGTGAAG CTCACGAGTT ACGAGATGAT CCCCCCATCA TTGTCATTGA AATCTACGAC
    CAGGACAGCA TGGGCAAAGC CGACTTCATG GGCCGGACCT TCGCCAAGCC CCTGGTGAAG
    ATGGCAGATG AAGCATACTG CCCACCTCGC TTCCCGCCGC AGCTTGAGTA CTACCAGATC
    TACCGAGGCA GTGCCACTGC CGGAGACCTA CTGGCTGCCT TCGAGCTGCT GCAGATTGGG
    CCATCAGGGA AGGCTGACCT GCCACCCATC AATGGCCCAG TGGACATGGA CAGAGGGCCC
    ATCATGCCTG TGCCCGTGGG AATCCGGCCA GTGCTCAGCA AGTACCGAGT GGAGGTGCTG
    TTCTGGGGCC TGAGGGACCT AAAGAGGGTG AACCTGGCCC AGGTGGACCG ACCACGGGTG
    GACATCGAGT GTGCAGGAAA GGGGGTACAA TCCTCCCTGA TTCACAATTA TAAGAAGAAC
    CCCAACTTCA ACACGCTGGT CAAGTGGTTT GAAGTGGACC TCCCGGAGAA TGAGCTCCTG
    CACCCACCCT TGAACATCCG AGTGGTAGAT TGCCGGGCCT TTGGACGATA CACCCTGGTG
    GGTTCCCACG CAGTCAGCTC ACTGAGGCGC TTCATCTACC GACCTCCAGA CCGCTCAGCC
    CCCAACTGGA ACACCACAGT CAGGCTGCTC CGGGGCTGCC ACAGGCTGCG CAATGGGGGC
    CCCTCTTCTC GCCCCACAGG GGAGGTTGTA GTAAGCATGG AGCCTGAGGA GCCAGTTAAG
    AAGCTGGAGA CCATGGTGAA ACTGGATGCG ACTTCTGATG CTGTGGTCAA GGTGGATGTG
    GCTGAAGATG AGAAGGAAAG GAAGAAGAAG AAAAAGAAAG GCCCGTCAGA GGAGCCAGAG
    GAGGAAGAGC CCGATGAGAG CATGCTGGAT TGGTGGTCCA AGTACTTCGC CTCCATCGAC
    ACAATGAAGG AGCAACTTCG ACAACATGAG ACCTCTGGAA CTGACTTGGA AGAGAAGGAA
    GAGATGGAAA GCGCTGAGGG CCTGAAGGGA CCAATGAAGA GCAAGGAGAA GTCCAGAGCT
    GCAAAGGAGG AGAAAAAGAA GAAAAACCAG AGCCCTGGCC CTGGCCAGGG ATCGGAGGCT
    CCTGAGAAGA AGAAAGCCAA GATCGATGAG CTTAAGGTGT ACCCCAAGGA GCTGGAATCG
    GAGTTTGACA GCTTTGAGGA CTGGCTGCAC ACCTTCAACC TGTTGAGGGG CAAGACGGGA
    GATGATGAGG ATGGCTCCAC AGAGGAGGAG CGCATAGTAG GCCGATTCAA GGGCTCCCTC
    TGTGTGTACA AAGTGCCACT CCCAGAAGAT GTATCTCGAG AAGCTGGCTA TGATCCCACC
    TATGGAATGT TCCAGGGCAT CCCAAGCAAT GACCCCATCA ATGTGCTGGT CCGAATCTAT
    GTGGTCCGGG CCACAGACCT GCACCCGGCC GACATCAATG GCAAAGCTGA CCCCTATATT
    GCCATCAAGT TAGGCAAGAC CGACATCCGA GACAAGGAGA ACTACATCTC CAAGCAGCTC
    AACCCTGTGT TTGGGAAGTC CTTTGACATT GAGGCCTCCT TCCCCATGGA GTCCATGTTG
    ACAGTGGCCG TGTACGACTG GGATCTGGTG GGCACTGATG ACCTCATCGG AGAAACCAAG
    ATTGACCTGG AAAACCGCTT CTACAGCAAG CATCGCGCCA CCTGCGGCAT CGCACAGACC
    TATTCCATAC ATGGCTACAA TATCTGGAGG GACCCCATGA AGCCCAGCCA GATCCTGACA
    CGCCTCTGTA AAGAGGGCAA AGTGGACGGC CCCCACTTTG GTCCCCATGG GAGAGTGAGG
    GTTGCCAACC GTGTCTTCAC GGGGCCTTCA GAAATAGAGG ATGAGAATGG TCAGAGGAAG
    CCCACAGATG AGCACGTGGC ACTGTCTGCT CTGAGACACT GGGAGGACAT CCCCCGGGTG
    GGCTGCCGCC TTGTGCCGGA ACACGTGGAG ACCAGGCCGC TGCTCAACCC TGACAAGCCA
    GGCATTGAGC AGGGCCGCCT GGAGCTGTGG GTGGACATGT TCCCCATGGA CATGCCAGCC
    CCTGGGACAC CTCTGGATAT ATCCCCCAGG AAACCCAAGA AGTACGAGCT GCGGGTCATC
    GTGTGGAACA CAGACGAGGT GGTCCTGGAA GACGATGATT TCTTCACGGG AGAGAAGTCC
    AGTGACATTT TTGTGAGGGG GTGGCTGAAG GGCCAGCAGG AGGACAAACA GGACACAGAT
    GTCCACTATC ACTCCCTCAC GGGGGAGGGC AACTTCAACT GGAGATACCT CTTCCCCTTC
    GACTACCTAG CGGCCGAAGA GAAGATCGTT ATGTCCAAAA AGGAGTCTAT GTTCTCCTGG
    GATGAGACGG AGTACAAGAT CCCTGCGCGG CTCACCCTGC AGATCTGGGA CGCTGACCAC
    TTCTCGGCTG ACGACTTCCT GGGGGCTATC GAGCTGGACC TGAACCGGTT CCCGAGGGGC
    GCTAAGACAG CCAAGCAGTG CACCATGGAG ATGGCCACCG GGGAGGTGGA CGTACCCCTG
    GTTTCCATCT TTAAACAGAA ACGTGTCAAA GGCTGGTGGC CCCTCCTGGC CCGCAATGAG
    AATGATGAGT TTGAGCTCAC AGGCAAAGTG GAGGCGGAGC TACACCTACT CACGGCAGAG
    GAGGCAGAGA AGAACCCTGT GGGCCTGGCT CGCAATGAAC CTGATCCCCT AGAAAAACCC
    AATCGGCCGG ACACAAGCTT CATCTGGTTC TTGAACCCTC TCAAGTCTGC CCGCTACTTC
    CTGTGGCATA CCTACCGCTG GCTACTCCTC AAATTCCTGC TGCTCTTCCT CCTGCTGCTG
    CTCTTCGCCC TGTTTCTCTA CTCTCTGCCT GGCTACCTGG CCAAGAAGAT CCTTGGGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006504272.1
    CDS79..5541
    Translation

    Target ORF information:

    RefSeq Version XM_006504209.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus otoferlin (Otof), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006504209.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAACTCG GCAAAACTCG GTCCCACAAA GAGGAGCCCC AAAGACAAGA TGAGCCAGCA 
    GTGCTGGAGA TGGAGGACCT GGACCACCTA GCCATTCAGC TGGGGGATGG GCTGGATCCT
    GACTCCGTGT CTCTAGCCTC GGTCACCGCT CTCACCAGCA ATGTCTCCAA CAAACGGTCT
    AAGCCAGATA TTAAGATGGA GCCCAGTGCT GGAAGGCCCA TGGATTACCA GGTCAGCATC
    ACAGTGATTG AGGCTCGGCA GCTGGTGGGC TTGAACATGG ACCCTGTGGT GTGTGTGGAG
    GTGGGTGATG ACAAGAAATA CACGTCAATG AAGGAGTCCA CAAACTGCCC TTACTACAAC
    GAGTACTTTG TCTTCGACTT CCATGTCTCT CCTGATGTCA TGTTTGACAA GATCATCAAG
    ATCTCGGTTA TCCATTCTAA GAACCTGCTT CGGAGCGGCA CCCTGGTGGG TTCCTTCAAA
    ATGGATGTGG GGACTGTGTA TTCCCAGCCT GAACACCAGT TCCATCACAA ATGGGCCATC
    CTGTCAGACC CCGATGACAT CTCTGCTGGG TTGAAGGGTT ATGTAAAGTG TGATGTCGCT
    GTGGTGGGCA AGGGAGACAA CATCAAGACA CCCCACAAGG CCAACGAGAC GGATGAGGAC
    GACATTGAAG GGAACTTGCT GCTCCCCGAG GGCGTGCCCC CCGAACGGCA GTGGGCACGG
    TTCTATGTGA AAATTTACCG AGCAGAGGGA CTGCCCCGGA TGAACACAAG CCTCATGGCC
    AACGTGAAGA AGGCGTTCAT CGGTGAGAAC AAGGACCTCG TCGACCCCTA TGTGCAAGTC
    TTCTTTGCTG GACAAAAGGG CAAAACATCA GTGCAGAAGA GCAGCTATGA GCCGCTATGG
    AATGAGCAGG TCGTCTTCAC AGACTTGTTC CCCCCACTCT GCAAACGCAT GAAGGTGCAG
    ATCCGGGACT CTGACAAGGT CAATGATGTG GCCATCGGCA CCCACTTCAT CGACCTGCGC
    AAGATTTCCA ACGATGGAGA CAAAGGCTTC CTGCCTACCC TCGGTCCAGC CTGGGTGAAC
    ATGTACGGCT CCACGCGCAA CTACACACTG CTGGACGAGC ACCAGGACTT GAATGAAGGC
    CTGGGGGAGG GTGTGTCCTT CCGGGCCCGC CTCATGTTGG GACTAGCTGT GGAGATCCTG
    GACACCTCCA ACCCAGAGCT CACCAGCTCC ACGGAGGTGC AGGTGGAGCA GGCCACGCCT
    GTCTCGGAGA GCTGCACAGG GAGAATGGAA GAATTTTTTC TATTTGGAGC CTTCTTGGAA
    GCCTCAATGA TTGACCGGAA AAATGGGGAC AAGCCAATTA CCTTTGAGGT GACCATAGGA
    AACTACGGCA ATGAAGTCGA TGGTATGTCC CGGCCCCTGA GGCCTCGGCC CCGGAAAGAG
    CCTGGGGATG AAGAAGAGGT AGACCTGATT CAGAACTCCA GTGACGATGA AGGTGACGAA
    GCCGGGGACC TGGCCTCGGT GTCCTCCACC CCACCTATGC GGCCCCAGAT CACGGACAGG
    AACTATTTCC ACCTGCCCTA CCTGGAGCGC AAGCCCTGCA TCTATATCAA GAGCTGGTGG
    CCTGACCAGA GGCGGCGCCT CTACAATGCC AACATCATGG ATCACATTGC TGACAAGCTG
    GAAGAAGGCC TGAATGATGT ACAGGAGATG ATCAAAACGG AGAAGTCCTA CCCGGAGCGC
    CGCCTGCGGG GTGTGCTAGA GGAACTCAGC TGTGGCTGCC ACCGCTTCCT CTCCCTCTCG
    GACAAGGACC AGGGCCGCTC GTCCCGCACC AGGCTGGATC GAGAGCGTCT TAAGTCCTGT
    ATGAGGGAGT TGGAGAGCAT GGGACAGCAG GCCAAGAGCC TGAGGGCTCA GGTGAAGCGG
    CACACTGTTC GGGACAAGCT GAGGTCATGC CAGAACTTTC TGCAGAAGCT ACGCTTCCTG
    GCGGATGAGC CCCAGCACAG CATTCCTGAT GTGTTCATTT GGATGATGAG CAACAACAAA
    CGTATCGCCT ATGCCCGCGT GCCTTCCAAA GACCTGCTCT TCTCCATCGT GGAGGAGGAA
    CTGGGCAAGG ACTGCGCCAA AGTCAAGACC CTCTTCCTGA AGCTGCCAGG GAAGAGGGGC
    TTCGGCTCGG CAGGCTGGAC AGTACAGGCC AAGCTGGAGC TCTACCTGTG GCTGGGCCTC
    AGCAAGCAGC GAAAGGACTT CCTGTGTGGT CTGCCCTGTG GCTTCGAGGA GGTCAAGGCA
    GCCCAAGGCC TGGGCCTGCA TTCCTTTCCG CCCATCAGCC TAGTCTACAC CAAGAAGCAA
    GCCTTCCAGC TCCGAGCACA CATGTATCAG GCCCGAAGCC TCTTTGCTGC TGACAGCAGT
    GGGCTCTCTG ATCCCTTTGC CCGTGTCTTC TTCATCAACC AGAGCCAATG CACTGAGGTT
    CTAAACGAGA CACTGTGTCC CACCTGGGAC CAGATGCTGG TATTTGACAA CCTGGAGCTG
    TACGGTGAAG CTCACGAGTT ACGAGATGAT CCCCCCATCA TTGTCATTGA AATCTACGAC
    CAGGACAGCA TGGGCAAAGC CGACTTCATG GGCCGGACCT TCGCCAAGCC CCTGGTGAAG
    ATGGCAGATG AAGCATACTG CCCACCTCGC TTCCCGCCGC AGCTTGAGTA CTACCAGATC
    TACCGAGGCA GTGCCACTGC CGGAGACCTA CTGGCTGCCT TCGAGCTGCT GCAGATTGGG
    CCATCAGGGA AGGCTGACCT GCCACCCATC AATGGCCCAG TGGACATGGA CAGAGGGCCC
    ATCATGCCTG TGCCCGTGGG AATCCGGCCA GTGCTCAGCA AGTACCGAGT GGAGGTGCTG
    TTCTGGGGCC TGAGGGACCT AAAGAGGGTG AACCTGGCCC AGGTGGACCG ACCACGGGTG
    GACATCGAGT GTGCAGGAAA GGGGGTACAA TCCTCCCTGA TTCACAATTA TAAGAAGAAC
    CCCAACTTCA ACACGCTGGT CAAGTGGTTT GAAGTGGACC TCCCGGAGAA TGAGCTCCTG
    CACCCACCCT TGAACATCCG AGTGGTAGAT TGCCGGGCCT TTGGACGATA CACCCTGGTG
    GGTTCCCACG CAGTCAGCTC ACTGAGGCGC TTCATCTACC GACCTCCAGA CCGCTCAGCC
    CCCAACTGGA ACACCACAGT CAGGCTGCTC CGGGGCTGCC ACAGGCTGCG CAATGGGGGC
    CCCTCTTCTC GCCCCACAGG GGAGGTTGTA GTAAGCATGG AGCCTGAGGA GCCAGTTAAG
    AAGCTGGAGA CCATGGTGAA ACTGGATGCG ACTTCTGATG CTGTGGTCAA GGTGGATGTG
    GCTGAAGATG AGAAGGAAAG GAAGAAGAAG AAAAAGAAAG GCCCGTCAGA GGAGCCAGAG
    GAGGAAGAGC CCGATGAGAG CATGCTGGAT TGGTGGTCCA AGTACTTCGC CTCCATCGAC
    ACAATGAAGG AGCAACTTCG ACAACATGAG ACCTCTGGAA CTGACTTGGA AGAGAAGGAA
    GAGATGGAAA GCGCTGAGGG CCTGAAGGGA CCAATGAAGA GCAAGGAGAA GTCCAGAGCT
    GCAAAGGAGG AGAAAAAGAA GAAAAACCAG AGCCCTGGCC CTGGCCAGGG ATCGGAGGCT
    CCTGAGAAGA AGAAAGCCAA GATCGATGAG CTTAAGGTGT ACCCCAAGGA GCTGGAATCG
    GAGTTTGACA GCTTTGAGGA CTGGCTGCAC ACCTTCAACC TGTTGAGGGG CAAGACGGGA
    GATGATGAGG ATGGCTCCAC AGAGGAGGAG CGCATAGTAG GCCGATTCAA GGGCTCCCTC
    TGTGTGTACA AAGTGCCACT CCCAGAAGAT GTATCTCGAG AAGCTGGCTA TGATCCCACC
    TATGGAATGT TCCAGGGCAT CCCAAGCAAT GACCCCATCA ATGTGCTGGT CCGAATCTAT
    GTGGTCCGGG CCACAGACCT GCACCCGGCC GACATCAATG GCAAAGCTGA CCCCTATATT
    GCCATCAAGT TAGGCAAGAC CGACATCCGA GACAAGGAGA ACTACATCTC CAAGCAGCTC
    AACCCTGTGT TTGGGAAGTC CTTTGACATT GAGGCCTCCT TCCCCATGGA GTCCATGTTG
    ACAGTGGCCG TGTACGACTG GGATCTGGTG GGCACTGATG ACCTCATCGG AGAAACCAAG
    ATTGACCTGG AAAACCGCTT CTACAGCAAG CATCGCGCCA CCTGCGGCAT CGCACAGACC
    TATTCCATAC ATGGCTACAA TATCTGGAGG GACCCCATGA AGCCCAGCCA GATCCTGACA
    CGCCTCTGTA AAGAGGGCAA AGTGGACGGC CCCCACTTTG GTCCCCATGG GAGAGTGAGG
    GTTGCCAACC GTGTCTTCAC GGGGCCTTCA GAAATAGAGG ATGAGAATGG TCAGAGGAAG
    CCCACAGATG AGCACGTGGC ACTGTCTGCT CTGAGACACT GGGAGGACAT CCCCCGGGTG
    GGCTGCCGCC TTGTGCCGGA ACACGTGGAG ACCAGGCCGC TGCTCAACCC TGACAAGCCA
    GGCATTGAGC AGGGCCGCCT GGAGCTGTGG GTGGACATGT TCCCCATGGA CATGCCAGCC
    CCTGGGACAC CTCTGGATAT ATCCCCCAGG AAACCCAAGA AGTACGAGCT GCGGGTCATC
    GTGTGGAACA CAGACGAGGT GGTCCTGGAA GACGATGATT TCTTCACGGG AGAGAAGTCC
    AGTGACATTT TTGTGAGGGG GTGGCTGAAG GGCCAGCAGG AGGACAAACA GGACACAGAT
    GTCCACTATC ACTCCCTCAC GGGGGAGGGC AACTTCAACT GGAGATACCT CTTCCCCTTC
    GACTACCTAG CGGCCGAAGA GAAGATCGTT ATGTCCAAAA AGGAGTCTAT GTTCTCCTGG
    GATGAGACGG AGTACAAGAT CCCTGCGCGG CTCACCCTGC AGATCTGGGA CGCTGACCAC
    TTCTCGGCTG ACGACTTCCT GGGGGCTATC GAGCTGGACC TGAACCGGTT CCCGAGGGGC
    GCTAAGACAG CCAAGCAGTG CACCATGGAG ATGGCCACCG GGGAGGTGGA CGTACCCCTG
    GTTTCCATCT TTAAACAGAA ACGTGTCAAA GGCTGGTGGC CCCTCCTGGC CCGCAATGAG
    AATGATGAGT TTGAGCTCAC AGGCAAAGTG GAGGCGGAGC TACACCTACT CACGGCAGAG
    GAGGCAGAGA AGAACCCTGT GGGCCTGGCT CGCAATGAAC CTGATCCCCT AGAAAAACCC
    AATCGGCCGG ACACAAGCTT CATCTGGTTC TTGAACCCTC TCAAGTCTGC CCGCTACTTC
    CTGTGGCATA CCTACCGCTG GCTACTCCTC AAATTCCTGC TGCTCTTCCT CCTGCTGCTG
    CTCTTCGCCC TGTTTCTCTA CTCTCTGCCT GGCTACCTGG CCAAGAAGAT CCTTGGGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu81542
    Clone ID Related Accession (Same CDS sequence) XM_017321186.1
    Accession Version XM_017321186.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5970bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product otoferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGGAC CTCCCGGAGA ATGAGCTCCT GCACCCACCC
    TTGAACATCC GAGTGGTAGA TTGCCGGGCC TTTGGACGAT ACACCCTGGT GGGTTCCCAC
    GCAGTCAGCT CACTGAGGCG CTTCATCTAC CGACCTCCAG ACCGCTCAGC CCCCAACTGG
    AACACCACAG TCAGGCTGCT CCGGGGCTGC CACAGGCTGC GCAATGGGGG CCCCTCTTCT
    CGCCCCACAG GGGAGGTTGT AGTAAGCATG GAGCCTGAGG AGCCAGTTAA GAAGCTGGAG
    ACCATGGTGA AACTGGATGC GACTTCTGAT GCTGTGGTCA AGGTGGATGT GGCTGAAGAT
    GAGAAGGAAA GGAAGAAGAA GAAAAAGAAA GGCCCGTCAG AGGAGCCAGA GGAGGAAGAG
    CCCGATGAGA GCATGCTGGA TTGGTGGTCC AAGTACTTCG CCTCCATCGA CACAATGAAG
    GAGCAACTTC GACAACATGA GACCTCTGGA ACTGACTTGG AAGAGAAGGA AGAGATGGAA
    AGCGCTGAGG GCCTGAAGGG ACCAATGAAG AGCAAGGAGA AGTCCAGAGC TGCAAAGGAG
    GAGAAAAAGA AGAAAAACCA GAGCCCTGGC CCTGGCCAGG GATCGGAGGC TCCTGAGAAG
    AAGAAAGCCA AGATCGATGA GCTTAAGGTG TACCCCAAGG AGCTGGAATC GGAGTTTGAC
    AGCTTTGAGG ACTGGCTGCA CACCTTCAAC CTGTTGAGGG GCAAGACGGG AGATGATGAG
    GATGGCTCCA CAGAGGAGGA GCGCATAGTA GGCCGATTCA AGGGCTCCCT CTGTGTGTAC
    AAAGTGCCAC TCCCAGAAGA TGTATCTCGA GAAGCTGGCT ATGATCCCAC CTATGGAATG
    TTCCAGGGCA TCCCAAGCAA TGACCCCATC AATGTGCTGG TCCGAATCTA TGTGGTCCGG
    GCCACAGACC TGCACCCGGC CGACATCAAT GGCAAAGCTG ACCCCTATAT TGCCATCAAG
    TTAGGCAAGA CCGACATCCG AGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTG
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AGTCCATGTT GACAGTGGCC
    GTGTACGACT GGGATCTGGT GGGCACTGAT GACCTCATCG GAGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCATCGCGCC ACCTGCGGCA TCGCACAGAC CTATTCCATA
    CATGGCTACA ATATCTGGAG GGACCCCATG AAGCCCAGCC AGATCCTGAC ACGCCTCTGT
    AAAGAGGGCA AAGTGGACGG CCCCCACTTT GGTCCCCATG GGAGAGTGAG GGTTGCCAAC
    CGTGTCTTCA CGGGGCCTTC AGAAATAGAG GATGAGAATG GTCAGAGGAA GCCCACAGAT
    GAGCACGTGG CACTGTCTGC TCTGAGACAC TGGGAGGACA TCCCCCGGGT GGGCTGCCGC
    CTTGTGCCGG AACACGTGGA GACCAGGCCG CTGCTCAACC CTGACAAGCC AGGCATTGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACA
    CCTCTGGATA TATCCCCCAG GAAACCCAAG AAGTACGAGC TGCGGGTCAT CGTGTGGAAC
    ACAGACGAGG TGGTCCTGGA AGACGATGAT TTCTTCACGG GAGAGAAGTC CAGTGACATT
    TTTGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAAC AGGACACAGA TGTCCACTAT
    CACTCCCTCA CGGGGGAGGG CAACTTCAAC TGGAGATACC TCTTCCCCTT CGACTACCTA
    GCGGCCGAAG AGAAGATCGT TATGTCCAAA AAGGAGTCTA TGTTCTCCTG GGATGAGACG
    GAGTACAAGA TCCCTGCGCG GCTCACCCTG CAGATCTGGG ACGCTGACCA CTTCTCGGCT
    GACGACTTCC TGGGGGCTAT CGAGCTGGAC CTGAACCGGT TCCCGAGGGG CGCTAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTACCCCT GGTTTCCATC
    TTTAAACAGA AACGTGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAATGATGAG
    TTTGAGCTCA CAGGCAAAGT GGAGGCGGAG CTACACCTAC TCACGGCAGA GGAGGCAGAG
    AAGAACCCTG TGGGCCTGGC TCGCAATGAA CCTGATCCCC TAGAAAAACC CAAACCCATC
    ATGGGCTTCA ACCCTTCAAC CCTTCCTTCA ACCCCGAAGC TACCTCTTCC CTCTGAACCA
    AAAGAAGCTC ATCTTCAAAG AAAGCTGTTG CTTTCCCAGG AAGCCCAGCC CCCAAGTCTG
    ACAGTGGTAT GGGTTCTTCC TAGTTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176675.1
    CDS917..6886
    Translation

    Target ORF information:

    RefSeq Version XM_017321186.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus otoferlin (Otof), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321186.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGGAC CTCCCGGAGA ATGAGCTCCT GCACCCACCC
    TTGAACATCC GAGTGGTAGA TTGCCGGGCC TTTGGACGAT ACACCCTGGT GGGTTCCCAC
    GCAGTCAGCT CACTGAGGCG CTTCATCTAC CGACCTCCAG ACCGCTCAGC CCCCAACTGG
    AACACCACAG TCAGGCTGCT CCGGGGCTGC CACAGGCTGC GCAATGGGGG CCCCTCTTCT
    CGCCCCACAG GGGAGGTTGT AGTAAGCATG GAGCCTGAGG AGCCAGTTAA GAAGCTGGAG
    ACCATGGTGA AACTGGATGC GACTTCTGAT GCTGTGGTCA AGGTGGATGT GGCTGAAGAT
    GAGAAGGAAA GGAAGAAGAA GAAAAAGAAA GGCCCGTCAG AGGAGCCAGA GGAGGAAGAG
    CCCGATGAGA GCATGCTGGA TTGGTGGTCC AAGTACTTCG CCTCCATCGA CACAATGAAG
    GAGCAACTTC GACAACATGA GACCTCTGGA ACTGACTTGG AAGAGAAGGA AGAGATGGAA
    AGCGCTGAGG GCCTGAAGGG ACCAATGAAG AGCAAGGAGA AGTCCAGAGC TGCAAAGGAG
    GAGAAAAAGA AGAAAAACCA GAGCCCTGGC CCTGGCCAGG GATCGGAGGC TCCTGAGAAG
    AAGAAAGCCA AGATCGATGA GCTTAAGGTG TACCCCAAGG AGCTGGAATC GGAGTTTGAC
    AGCTTTGAGG ACTGGCTGCA CACCTTCAAC CTGTTGAGGG GCAAGACGGG AGATGATGAG
    GATGGCTCCA CAGAGGAGGA GCGCATAGTA GGCCGATTCA AGGGCTCCCT CTGTGTGTAC
    AAAGTGCCAC TCCCAGAAGA TGTATCTCGA GAAGCTGGCT ATGATCCCAC CTATGGAATG
    TTCCAGGGCA TCCCAAGCAA TGACCCCATC AATGTGCTGG TCCGAATCTA TGTGGTCCGG
    GCCACAGACC TGCACCCGGC CGACATCAAT GGCAAAGCTG ACCCCTATAT TGCCATCAAG
    TTAGGCAAGA CCGACATCCG AGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTG
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AGTCCATGTT GACAGTGGCC
    GTGTACGACT GGGATCTGGT GGGCACTGAT GACCTCATCG GAGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCATCGCGCC ACCTGCGGCA TCGCACAGAC CTATTCCATA
    CATGGCTACA ATATCTGGAG GGACCCCATG AAGCCCAGCC AGATCCTGAC ACGCCTCTGT
    AAAGAGGGCA AAGTGGACGG CCCCCACTTT GGTCCCCATG GGAGAGTGAG GGTTGCCAAC
    CGTGTCTTCA CGGGGCCTTC AGAAATAGAG GATGAGAATG GTCAGAGGAA GCCCACAGAT
    GAGCACGTGG CACTGTCTGC TCTGAGACAC TGGGAGGACA TCCCCCGGGT GGGCTGCCGC
    CTTGTGCCGG AACACGTGGA GACCAGGCCG CTGCTCAACC CTGACAAGCC AGGCATTGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACA
    CCTCTGGATA TATCCCCCAG GAAACCCAAG AAGTACGAGC TGCGGGTCAT CGTGTGGAAC
    ACAGACGAGG TGGTCCTGGA AGACGATGAT TTCTTCACGG GAGAGAAGTC CAGTGACATT
    TTTGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAAC AGGACACAGA TGTCCACTAT
    CACTCCCTCA CGGGGGAGGG CAACTTCAAC TGGAGATACC TCTTCCCCTT CGACTACCTA
    GCGGCCGAAG AGAAGATCGT TATGTCCAAA AAGGAGTCTA TGTTCTCCTG GGATGAGACG
    GAGTACAAGA TCCCTGCGCG GCTCACCCTG CAGATCTGGG ACGCTGACCA CTTCTCGGCT
    GACGACTTCC TGGGGGCTAT CGAGCTGGAC CTGAACCGGT TCCCGAGGGG CGCTAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTACCCCT GGTTTCCATC
    TTTAAACAGA AACGTGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAATGATGAG
    TTTGAGCTCA CAGGCAAAGT GGAGGCGGAG CTACACCTAC TCACGGCAGA GGAGGCAGAG
    AAGAACCCTG TGGGCCTGGC TCGCAATGAA CCTGATCCCC TAGAAAAACC CAAACCCATC
    ATGGGCTTCA ACCCTTCAAC CCTTCCTTCA ACCCCGAAGC TACCTCTTCC CTCTGAACCA
    AAAGAAGCTC ATCTTCAAAG AAAGCTGTTG CTTTCCCAGG AAGCCCAGCC CCCAAGTCTG
    ACAGTGGTAT GGGTTCTTCC TAGTTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16363
    Clone ID Related Accession (Same CDS sequence) NM_001100395.1
    Accession Version NM_001100395.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5979bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Mus musculus(house mouse)
    Product otoferlin isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK046460.1, AK147256.1, AC157761.6 and AK033317.1. Transcript Variant: This variant (1) contains multiple coding region differences resulting in different 3' coding region and 3' UTR, compared to variant 2. It encodes isoform 1, which is shorter and has a distinct C-terminus, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF183183.1, AY586513.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGCAAA GGCAGAGAGA AGACCAAGGG AGGCAGAGAT
    GGCGAGCACA AAGCGGGAAG GAGTGTGTTC TCGGCCATGA AACTCGGCAA AACTCGGTCC
    CACAAAGAGG AGCCCCAAAG ACAAGATGAG CCAGCAGTGC TGGAGATGGA GGACCTGGAC
    CACCTAGCCA TTCAGCTGGG GGATGGGCTG GATCCTGACT CCGTGTCTCT AGCCTCGGTC
    ACCGCTCTCA CCAGCAATGT CTCCAACAAA CGGTCTAAGC CAGATATTAA GATGGAGCCC
    AGTGCTGGAA GGCCCATGGA TTACCAGGTC AGCATCACAG TGATTGAGGC TCGGCAGCTG
    GTGGGCTTGA ACATGGACCC TGTGGTGTGT GTGGAGGTGG GTGATGACAA GAAATACACG
    TCAATGAAGG AGTCCACAAA CTGCCCTTAC TACAACGAGT ACTTTGTCTT CGACTTCCAT
    GTCTCTCCTG ATGTCATGTT TGACAAGATC ATCAAGATCT CGGTTATCCA TTCTAAGAAC
    CTGCTTCGGA GCGGCACCCT GGTGGGTTCC TTCAAAATGG ATGTGGGGAC TGTGTATTCC
    CAGCCTGAAC ACCAGTTCCA TCACAAATGG GCCATCCTGT CAGACCCCGA TGACATCTCT
    GCTGGGTTGA AGGGTTATGT AAAGTGTGAT GTCGCTGTGG TGGGCAAGGG AGACAACATC
    AAGACACCCC ACAAGGCCAA CGAGACGGAT GAGGACGACA TTGAAGGGAA CTTGCTGCTC
    CCCGAGGGCG TGCCCCCCGA ACGGCAGTGG GCACGGTTCT ATGTGAAAAT TTACCGAGCA
    GAGGGACTGC CCCGGATGAA CACAAGCCTC ATGGCCAACG TGAAGAAGGC GTTCATCGGT
    GAGAACAAGG ACCTCGTCGA CCCCTATGTG CAAGTCTTCT TTGCTGGACA AAAGGGCAAA
    ACATCAGTGC AGAAGAGCAG CTATGAGCCG CTATGGAATG AGCAGGTCGT CTTCACAGAC
    TTGTTCCCCC CACTCTGCAA ACGCATGAAG GTGCAGATCC GGGACTCTGA CAAGGTCAAT
    GATGTGGCCA TCGGCACCCA CTTCATCGAC CTGCGCAAGA TTTCCAACGA TGGAGACAAA
    GGCTTCCTGC CTACCCTCGG TCCAGCCTGG GTGAACATGT ACGGCTCCAC GCGCAACTAC
    ACACTGCTGG ACGAGCACCA GGACTTGAAT GAAGGCCTGG GGGAGGGTGT GTCCTTCCGG
    GCCCGCCTCA TGTTGGGACT AGCTGTGGAG ATCCTGGACA CCTCCAACCC AGAGCTCACC
    AGCTCCACGG AGGTGCAGGT GGAGCAGGCC ACGCCTGTCT CGGAGAGCTG CACAGGGAGA
    ATGGAAGAAT TTTTTCTATT TGGAGCCTTC TTGGAAGCCT CAATGATTGA CCGGAAAAAT
    GGGGACAAGC CAATTACCTT TGAGGTGACC ATAGGAAACT ACGGCAATGA AGTCGATGGT
    ATGTCCCGGC CCCTGAGGCC TCGGCCCCGG AAAGAGCCTG GGGATGAAGA AGAGGTAGAC
    CTGATTCAGA ACTCCAGTGA CGATGAAGGT GACGAAGCCG GGGACCTGGC CTCGGTGTCC
    TCCACCCCAC CTATGCGGCC CCAGATCACG GACAGGAACT ATTTCCACCT GCCCTACCTG
    GAGCGCAAGC CCTGCATCTA TATCAAGAGC TGGTGGCCTG ACCAGAGGCG GCGCCTCTAC
    AATGCCAACA TCATGGATCA CATTGCTGAC AAGCTGGAAG AAGGCCTGAA TGATGTACAG
    GAGATGATCA AAACGGAGAA GTCCTACCCG GAGCGCCGCC TGCGGGGTGT GCTAGAGGAA
    CTCAGCTGTG GCTGCCACCG CTTCCTCTCC CTCTCGGACA AGGACCAGGG CCGCTCGTCC
    CGCACCAGGC TGGATCGAGA GCGTCTTAAG TCCTGTATGA GGGAGTTGGA GAGCATGGGA
    CAGCAGGCCA AGAGCCTGAG GGCTCAGGTG AAGCGGCACA CTGTTCGGGA CAAGCTGAGG
    TCATGCCAGA ACTTTCTGCA GAAGCTACGC TTCCTGGCGG ATGAGCCCCA GCACAGCATT
    CCTGATGTGT TCATTTGGAT GATGAGCAAC AACAAACGTA TCGCCTATGC CCGCGTGCCT
    TCCAAAGACC TGCTCTTCTC CATCGTGGAG GAGGAACTGG GCAAGGACTG CGCCAAAGTC
    AAGACCCTCT TCCTGAAGCT GCCAGGGAAG AGGGGCTTCG GCTCGGCAGG CTGGACAGTA
    CAGGCCAAGC TGGAGCTCTA CCTGTGGCTG GGCCTCAGCA AGCAGCGAAA GGACTTCCTG
    TGTGGTCTGC CCTGTGGCTT CGAGGAGGTC AAGGCAGCCC AAGGCCTGGG CCTGCATTCC
    TTTCCGCCCA TCAGCCTAGT CTACACCAAG AAGCAAGCCT TCCAGCTCCG AGCACACATG
    TATCAGGCCC GAAGCCTCTT TGCTGCTGAC AGCAGTGGGC TCTCTGATCC CTTTGCCCGT
    GTCTTCTTCA TCAACCAGAG CCAATGCACT GAGGTTCTAA ACGAGACACT GTGTCCCACC
    TGGGACCAGA TGCTGGTATT TGACAACCTG GAGCTGTACG GTGAAGCTCA CGAGTTACGA
    GATGATCCCC CCATCATTGT CATTGAAATC TACGACCAGG ACAGCATGGG CAAAGCCGAC
    TTCATGGGCC GGACCTTCGC CAAGCCCCTG GTGAAGATGG CAGATGAAGC ATACTGCCCA
    CCTCGCTTCC CGCCGCAGCT TGAGTACTAC CAGATCTACC GAGGCAGTGC CACTGCCGGA
    GACCTACTGG CTGCCTTCGA GCTGCTGCAG ATTGGGCCAT CAGGGAAGGC TGACCTGCCA
    CCCATCAATG GCCCAGTGGA CATGGACAGA GGGCCCATCA TGCCTGTGCC CGTGGGAATC
    CGGCCAGTGC TCAGCAAGTA CCGAGTGGAG GTGCTGTTCT GGGGCCTGAG GGACCTAAAG
    AGGGTGAACC TGGCCCAGGT GGACCGACCA CGGGTGGACA TCGAGTGTGC AGGAAAGGGG
    GTACAATCCT CCCTGATTCA CAATTATAAG AAGAACCCCA ACTTCAACAC GCTGGTCAAG
    TGGTTTGAAG TGGACCTCCC GGAGAATGAG CTCCTGCACC CACCCTTGAA CATCCGAGTG
    GTAGATTGCC GGGCCTTTGG ACGATACACC CTGGTGGGTT CCCACGCAGT CAGCTCACTG
    AGGCGCTTCA TCTACCGACC TCCAGACCGC TCAGCCCCCA ACTGGAACAC CACAGGGGAG
    GTTGTAGTAA GCATGGAGCC TGAGGAGCCA GTTAAGAAGC TGGAGACCAT GGTGAAACTG
    GATGCGACTT CTGATGCTGT GGTCAAGGTG GATGTGGCTG AAGATGAGAA GGAAAGGAAG
    AAGAAGAAAA AGAAAGGCCC GTCAGAGGAG CCAGAGGAGG AAGAGCCCGA TGAGAGCATG
    CTGGATTGGT GGTCCAAGTA CTTCGCCTCC ATCGACACAA TGAAGGAGCA ACTTCGACAA
    CATGAGACCT CTGGAACTGA CTTGGAAGAG AAGGAAGAGA TGGAAAGCGC TGAGGGCCTG
    AAGGGACCAA TGAAGAGCAA GGAGAAGTCC AGAGCTGCAA AGGAGGAGAA AAAGAAGAAA
    AACCAGAGCC CTGGCCCTGG CCAGGGATCG GAGGCTCCTG AGAAGAAGAA AGCCAAGATC
    GATGAGCTTA AGGTGTACCC CAAGGAGCTG GAATCGGAGT TTGACAGCTT TGAGGACTGG
    CTGCACACCT TCAACCTGTT GAGGGGCAAG ACGGGAGATG ATGAGGATGG CTCCACAGAG
    GAGGAGCGCA TAGTAGGCCG ATTCAAGGGC TCCCTCTGTG TGTACAAAGT GCCACTCCCA
    GAAGATGTAT CTCGAGAAGC TGGCTATGAT CCCACCTATG GAATGTTCCA GGGCATCCCA
    AGCAATGACC CCATCAATGT GCTGGTCCGA ATCTATGTGG TCCGGGCCAC AGACCTGCAC
    CCGGCCGACA TCAATGGCAA AGCTGACCCC TATATTGCCA TCAAGTTAGG CAAGACCGAC
    ATCCGAGACA AGGAGAACTA CATCTCCAAG CAGCTCAACC CTGTGTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAGTCC ATGTTGACAG TGGCCGTGTA CGACTGGGAT
    CTGGTGGGCA CTGATGACCT CATCGGAGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCATC GCGCCACCTG CGGCATCGCA CAGACCTATT CCATACATGG CTACAATATC
    TGGAGGGACC CCATGAAGCC CAGCCAGATC CTGACACGCC TCTGTAAAGA GGGCAAAGTG
    GACGGCCCCC ACTTTGGTCC CCATGGGAGA GTGAGGGTTG CCAACCGTGT CTTCACGGGG
    CCTTCAGAAA TAGAGGATGA GAATGGTCAG AGGAAGCCCA CAGATGAGCA CGTGGCACTG
    TCTGCTCTGA GACACTGGGA GGACATCCCC CGGGTGGGCT GCCGCCTTGT GCCGGAACAC
    GTGGAGACCA GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TTGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ACATGTTCCC CATGGACATG CCAGCCCCTG GGACACCTCT GGATATATCC
    CCCAGGAAAC CCAAGAAGTA CGAGCTGCGG GTCATCGTGT GGAACACAGA CGAGGTGGTC
    CTGGAAGACG ATGATTTCTT CACGGGAGAG AAGTCCAGTG ACATTTTTGT GAGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAACAGGAC ACAGATGTCC ACTATCACTC CCTCACGGGG
    GAGGGCAACT TCAACTGGAG ATACCTCTTC CCCTTCGACT ACCTAGCGGC CGAAGAGAAG
    ATCGTTATGT CCAAAAAGGA GTCTATGTTC TCCTGGGATG AGACGGAGTA CAAGATCCCT
    GCGCGGCTCA CCCTGCAGAT CTGGGACGCT GACCACTTCT CGGCTGACGA CTTCCTGGGG
    GCTATCGAGC TGGACCTGAA CCGGTTCCCG AGGGGCGCTA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACCGGGGA GGTGGACGTA CCCCTGGTTT CCATCTTTAA ACAGAAACGT
    GTCAAAGGCT GGTGGCCCCT CCTGGCCCGC AATGAGAATG ATGAGTTTGA GCTCACAGGC
    AAAGTGGAGG CGGAGCTACA CCTACTCACG GCAGAGGAGG CAGAGAAGAA CCCTGTGGGC
    CTGGCTCGCA ATGAACCTGA TCCCCTAGAA AAACCCAACC GGCCTGACAC GGCATTCGTC
    TGGTTCCTGA ACCCACTCAA ATCTATCAAG TACCTCATCT GCACCCGGTA CAAGTGGCTG
    ATCATCAAGA TCGTGCTGGC GCTGCTGGGG CTGCTCATGC TGGCCCTCTT CCTTTACAGC
    CTCCCAGGCT ACATGGTCAA GAAGCTCCTA GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001093865.1
    CDS135..6113
    Translation

    Target ORF information:

    RefSeq Version NM_001100395.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus otoferlin (Otof), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001100395.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGCAAA GGCAGAGAGA AGACCAAGGG AGGCAGAGAT
    GGCGAGCACA AAGCGGGAAG GAGTGTGTTC TCGGCCATGA AACTCGGCAA AACTCGGTCC
    CACAAAGAGG AGCCCCAAAG ACAAGATGAG CCAGCAGTGC TGGAGATGGA GGACCTGGAC
    CACCTAGCCA TTCAGCTGGG GGATGGGCTG GATCCTGACT CCGTGTCTCT AGCCTCGGTC
    ACCGCTCTCA CCAGCAATGT CTCCAACAAA CGGTCTAAGC CAGATATTAA GATGGAGCCC
    AGTGCTGGAA GGCCCATGGA TTACCAGGTC AGCATCACAG TGATTGAGGC TCGGCAGCTG
    GTGGGCTTGA ACATGGACCC TGTGGTGTGT GTGGAGGTGG GTGATGACAA GAAATACACG
    TCAATGAAGG AGTCCACAAA CTGCCCTTAC TACAACGAGT ACTTTGTCTT CGACTTCCAT
    GTCTCTCCTG ATGTCATGTT TGACAAGATC ATCAAGATCT CGGTTATCCA TTCTAAGAAC
    CTGCTTCGGA GCGGCACCCT GGTGGGTTCC TTCAAAATGG ATGTGGGGAC TGTGTATTCC
    CAGCCTGAAC ACCAGTTCCA TCACAAATGG GCCATCCTGT CAGACCCCGA TGACATCTCT
    GCTGGGTTGA AGGGTTATGT AAAGTGTGAT GTCGCTGTGG TGGGCAAGGG AGACAACATC
    AAGACACCCC ACAAGGCCAA CGAGACGGAT GAGGACGACA TTGAAGGGAA CTTGCTGCTC
    CCCGAGGGCG TGCCCCCCGA ACGGCAGTGG GCACGGTTCT ATGTGAAAAT TTACCGAGCA
    GAGGGACTGC CCCGGATGAA CACAAGCCTC ATGGCCAACG TGAAGAAGGC GTTCATCGGT
    GAGAACAAGG ACCTCGTCGA CCCCTATGTG CAAGTCTTCT TTGCTGGACA AAAGGGCAAA
    ACATCAGTGC AGAAGAGCAG CTATGAGCCG CTATGGAATG AGCAGGTCGT CTTCACAGAC
    TTGTTCCCCC CACTCTGCAA ACGCATGAAG GTGCAGATCC GGGACTCTGA CAAGGTCAAT
    GATGTGGCCA TCGGCACCCA CTTCATCGAC CTGCGCAAGA TTTCCAACGA TGGAGACAAA
    GGCTTCCTGC CTACCCTCGG TCCAGCCTGG GTGAACATGT ACGGCTCCAC GCGCAACTAC
    ACACTGCTGG ACGAGCACCA GGACTTGAAT GAAGGCCTGG GGGAGGGTGT GTCCTTCCGG
    GCCCGCCTCA TGTTGGGACT AGCTGTGGAG ATCCTGGACA CCTCCAACCC AGAGCTCACC
    AGCTCCACGG AGGTGCAGGT GGAGCAGGCC ACGCCTGTCT CGGAGAGCTG CACAGGGAGA
    ATGGAAGAAT TTTTTCTATT TGGAGCCTTC TTGGAAGCCT CAATGATTGA CCGGAAAAAT
    GGGGACAAGC CAATTACCTT TGAGGTGACC ATAGGAAACT ACGGCAATGA AGTCGATGGT
    ATGTCCCGGC CCCTGAGGCC TCGGCCCCGG AAAGAGCCTG GGGATGAAGA AGAGGTAGAC
    CTGATTCAGA ACTCCAGTGA CGATGAAGGT GACGAAGCCG GGGACCTGGC CTCGGTGTCC
    TCCACCCCAC CTATGCGGCC CCAGATCACG GACAGGAACT ATTTCCACCT GCCCTACCTG
    GAGCGCAAGC CCTGCATCTA TATCAAGAGC TGGTGGCCTG ACCAGAGGCG GCGCCTCTAC
    AATGCCAACA TCATGGATCA CATTGCTGAC AAGCTGGAAG AAGGCCTGAA TGATGTACAG
    GAGATGATCA AAACGGAGAA GTCCTACCCG GAGCGCCGCC TGCGGGGTGT GCTAGAGGAA
    CTCAGCTGTG GCTGCCACCG CTTCCTCTCC CTCTCGGACA AGGACCAGGG CCGCTCGTCC
    CGCACCAGGC TGGATCGAGA GCGTCTTAAG TCCTGTATGA GGGAGTTGGA GAGCATGGGA
    CAGCAGGCCA AGAGCCTGAG GGCTCAGGTG AAGCGGCACA CTGTTCGGGA CAAGCTGAGG
    TCATGCCAGA ACTTTCTGCA GAAGCTACGC TTCCTGGCGG ATGAGCCCCA GCACAGCATT
    CCTGATGTGT TCATTTGGAT GATGAGCAAC AACAAACGTA TCGCCTATGC CCGCGTGCCT
    TCCAAAGACC TGCTCTTCTC CATCGTGGAG GAGGAACTGG GCAAGGACTG CGCCAAAGTC
    AAGACCCTCT TCCTGAAGCT GCCAGGGAAG AGGGGCTTCG GCTCGGCAGG CTGGACAGTA
    CAGGCCAAGC TGGAGCTCTA CCTGTGGCTG GGCCTCAGCA AGCAGCGAAA GGACTTCCTG
    TGTGGTCTGC CCTGTGGCTT CGAGGAGGTC AAGGCAGCCC AAGGCCTGGG CCTGCATTCC
    TTTCCGCCCA TCAGCCTAGT CTACACCAAG AAGCAAGCCT TCCAGCTCCG AGCACACATG
    TATCAGGCCC GAAGCCTCTT TGCTGCTGAC AGCAGTGGGC TCTCTGATCC CTTTGCCCGT
    GTCTTCTTCA TCAACCAGAG CCAATGCACT GAGGTTCTAA ACGAGACACT GTGTCCCACC
    TGGGACCAGA TGCTGGTATT TGACAACCTG GAGCTGTACG GTGAAGCTCA CGAGTTACGA
    GATGATCCCC CCATCATTGT CATTGAAATC TACGACCAGG ACAGCATGGG CAAAGCCGAC
    TTCATGGGCC GGACCTTCGC CAAGCCCCTG GTGAAGATGG CAGATGAAGC ATACTGCCCA
    CCTCGCTTCC CGCCGCAGCT TGAGTACTAC CAGATCTACC GAGGCAGTGC CACTGCCGGA
    GACCTACTGG CTGCCTTCGA GCTGCTGCAG ATTGGGCCAT CAGGGAAGGC TGACCTGCCA
    CCCATCAATG GCCCAGTGGA CATGGACAGA GGGCCCATCA TGCCTGTGCC CGTGGGAATC
    CGGCCAGTGC TCAGCAAGTA CCGAGTGGAG GTGCTGTTCT GGGGCCTGAG GGACCTAAAG
    AGGGTGAACC TGGCCCAGGT GGACCGACCA CGGGTGGACA TCGAGTGTGC AGGAAAGGGG
    GTACAATCCT CCCTGATTCA CAATTATAAG AAGAACCCCA ACTTCAACAC GCTGGTCAAG
    TGGTTTGAAG TGGACCTCCC GGAGAATGAG CTCCTGCACC CACCCTTGAA CATCCGAGTG
    GTAGATTGCC GGGCCTTTGG ACGATACACC CTGGTGGGTT CCCACGCAGT CAGCTCACTG
    AGGCGCTTCA TCTACCGACC TCCAGACCGC TCAGCCCCCA ACTGGAACAC CACAGGGGAG
    GTTGTAGTAA GCATGGAGCC TGAGGAGCCA GTTAAGAAGC TGGAGACCAT GGTGAAACTG
    GATGCGACTT CTGATGCTGT GGTCAAGGTG GATGTGGCTG AAGATGAGAA GGAAAGGAAG
    AAGAAGAAAA AGAAAGGCCC GTCAGAGGAG CCAGAGGAGG AAGAGCCCGA TGAGAGCATG
    CTGGATTGGT GGTCCAAGTA CTTCGCCTCC ATCGACACAA TGAAGGAGCA ACTTCGACAA
    CATGAGACCT CTGGAACTGA CTTGGAAGAG AAGGAAGAGA TGGAAAGCGC TGAGGGCCTG
    AAGGGACCAA TGAAGAGCAA GGAGAAGTCC AGAGCTGCAA AGGAGGAGAA AAAGAAGAAA
    AACCAGAGCC CTGGCCCTGG CCAGGGATCG GAGGCTCCTG AGAAGAAGAA AGCCAAGATC
    GATGAGCTTA AGGTGTACCC CAAGGAGCTG GAATCGGAGT TTGACAGCTT TGAGGACTGG
    CTGCACACCT TCAACCTGTT GAGGGGCAAG ACGGGAGATG ATGAGGATGG CTCCACAGAG
    GAGGAGCGCA TAGTAGGCCG ATTCAAGGGC TCCCTCTGTG TGTACAAAGT GCCACTCCCA
    GAAGATGTAT CTCGAGAAGC TGGCTATGAT CCCACCTATG GAATGTTCCA GGGCATCCCA
    AGCAATGACC CCATCAATGT GCTGGTCCGA ATCTATGTGG TCCGGGCCAC AGACCTGCAC
    CCGGCCGACA TCAATGGCAA AGCTGACCCC TATATTGCCA TCAAGTTAGG CAAGACCGAC
    ATCCGAGACA AGGAGAACTA CATCTCCAAG CAGCTCAACC CTGTGTTTGG GAAGTCCTTT
    GACATTGAGG CCTCCTTCCC CATGGAGTCC ATGTTGACAG TGGCCGTGTA CGACTGGGAT
    CTGGTGGGCA CTGATGACCT CATCGGAGAA ACCAAGATTG ACCTGGAAAA CCGCTTCTAC
    AGCAAGCATC GCGCCACCTG CGGCATCGCA CAGACCTATT CCATACATGG CTACAATATC
    TGGAGGGACC CCATGAAGCC CAGCCAGATC CTGACACGCC TCTGTAAAGA GGGCAAAGTG
    GACGGCCCCC ACTTTGGTCC CCATGGGAGA GTGAGGGTTG CCAACCGTGT CTTCACGGGG
    CCTTCAGAAA TAGAGGATGA GAATGGTCAG AGGAAGCCCA CAGATGAGCA CGTGGCACTG
    TCTGCTCTGA GACACTGGGA GGACATCCCC CGGGTGGGCT GCCGCCTTGT GCCGGAACAC
    GTGGAGACCA GGCCGCTGCT CAACCCTGAC AAGCCAGGCA TTGAGCAGGG CCGCCTGGAG
    CTGTGGGTGG ACATGTTCCC CATGGACATG CCAGCCCCTG GGACACCTCT GGATATATCC
    CCCAGGAAAC CCAAGAAGTA CGAGCTGCGG GTCATCGTGT GGAACACAGA CGAGGTGGTC
    CTGGAAGACG ATGATTTCTT CACGGGAGAG AAGTCCAGTG ACATTTTTGT GAGGGGGTGG
    CTGAAGGGCC AGCAGGAGGA CAAACAGGAC ACAGATGTCC ACTATCACTC CCTCACGGGG
    GAGGGCAACT TCAACTGGAG ATACCTCTTC CCCTTCGACT ACCTAGCGGC CGAAGAGAAG
    ATCGTTATGT CCAAAAAGGA GTCTATGTTC TCCTGGGATG AGACGGAGTA CAAGATCCCT
    GCGCGGCTCA CCCTGCAGAT CTGGGACGCT GACCACTTCT CGGCTGACGA CTTCCTGGGG
    GCTATCGAGC TGGACCTGAA CCGGTTCCCG AGGGGCGCTA AGACAGCCAA GCAGTGCACC
    ATGGAGATGG CCACCGGGGA GGTGGACGTA CCCCTGGTTT CCATCTTTAA ACAGAAACGT
    GTCAAAGGCT GGTGGCCCCT CCTGGCCCGC AATGAGAATG ATGAGTTTGA GCTCACAGGC
    AAAGTGGAGG CGGAGCTACA CCTACTCACG GCAGAGGAGG CAGAGAAGAA CCCTGTGGGC
    CTGGCTCGCA ATGAACCTGA TCCCCTAGAA AAACCCAACC GGCCTGACAC GGCATTCGTC
    TGGTTCCTGA ACCCACTCAA ATCTATCAAG TACCTCATCT GCACCCGGTA CAAGTGGCTG
    ATCATCAAGA TCGTGCTGGC GCTGCTGGGG CTGCTCATGC TGGCCCTCTT CCTTTACAGC
    CTCCCAGGCT ACATGGTCAA GAAGCTCCTA GGGGCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16389
    Clone ID Related Accession (Same CDS sequence) NM_031875.2 , XM_017321185.1
    Accession Version NM_031875.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Mus musculus(house mouse)
    Product otoferlin isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK046460.1, AK147256.1, AK033317.1 and AC175379.4. On Jul 31, 2007 this sequence version replaced NM_031875.1. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF183184.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGGAC CTCCCGGAGA ATGAGCTCCT GCACCCACCC
    TTGAACATCC GAGTGGTAGA TTGCCGGGCC TTTGGACGAT ACACCCTGGT GGGTTCCCAC
    GCAGTCAGCT CACTGAGGCG CTTCATCTAC CGACCTCCAG ACCGCTCAGC CCCCAACTGG
    AACACCACAG TCAGGCTGCT CCGGGGCTGC CACAGGCTGC GCAATGGGGG CCCCTCTTCT
    CGCCCCACAG GGGAGGTTGT AGTAAGCATG GAGCCTGAGG AGCCAGTTAA GAAGCTGGAG
    ACCATGGTGA AACTGGATGC GACTTCTGAT GCTGTGGTCA AGGTGGATGT GGCTGAAGAT
    GAGAAGGAAA GGAAGAAGAA GAAAAAGAAA GGCCCGTCAG AGGAGCCAGA GGAGGAAGAG
    CCCGATGAGA GCATGCTGGA TTGGTGGTCC AAGTACTTCG CCTCCATCGA CACAATGAAG
    GAGCAACTTC GACAACATGA GACCTCTGGA ACTGACTTGG AAGAGAAGGA AGAGATGGAA
    AGCGCTGAGG GCCTGAAGGG ACCAATGAAG AGCAAGGAGA AGTCCAGAGC TGCAAAGGAG
    GAGAAAAAGA AGAAAAACCA GAGCCCTGGC CCTGGCCAGG GATCGGAGGC TCCTGAGAAG
    AAGAAAGCCA AGATCGATGA GCTTAAGGTG TACCCCAAGG AGCTGGAATC GGAGTTTGAC
    AGCTTTGAGG ACTGGCTGCA CACCTTCAAC CTGTTGAGGG GCAAGACGGG AGATGATGAG
    GATGGCTCCA CAGAGGAGGA GCGCATAGTA GGCCGATTCA AGGGCTCCCT CTGTGTGTAC
    AAAGTGCCAC TCCCAGAAGA TGTATCTCGA GAAGCTGGCT ATGATCCCAC CTATGGAATG
    TTCCAGGGCA TCCCAAGCAA TGACCCCATC AATGTGCTGG TCCGAATCTA TGTGGTCCGG
    GCCACAGACC TGCACCCGGC CGACATCAAT GGCAAAGCTG ACCCCTATAT TGCCATCAAG
    TTAGGCAAGA CCGACATCCG AGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTG
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AGTCCATGTT GACAGTGGCC
    GTGTACGACT GGGATCTGGT GGGCACTGAT GACCTCATCG GAGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCATCGCGCC ACCTGCGGCA TCGCACAGAC CTATTCCATA
    CATGGCTACA ATATCTGGAG GGACCCCATG AAGCCCAGCC AGATCCTGAC ACGCCTCTGT
    AAAGAGGGCA AAGTGGACGG CCCCCACTTT GGTCCCCATG GGAGAGTGAG GGTTGCCAAC
    CGTGTCTTCA CGGGGCCTTC AGAAATAGAG GATGAGAATG GTCAGAGGAA GCCCACAGAT
    GAGCACGTGG CACTGTCTGC TCTGAGACAC TGGGAGGACA TCCCCCGGGT GGGCTGCCGC
    CTTGTGCCGG AACACGTGGA GACCAGGCCG CTGCTCAACC CTGACAAGCC AGGCATTGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACA
    CCTCTGGATA TATCCCCCAG GAAACCCAAG AAGTACGAGC TGCGGGTCAT CGTGTGGAAC
    ACAGACGAGG TGGTCCTGGA AGACGATGAT TTCTTCACGG GAGAGAAGTC CAGTGACATT
    TTTGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAAC AGGACACAGA TGTCCACTAT
    CACTCCCTCA CGGGGGAGGG CAACTTCAAC TGGAGATACC TCTTCCCCTT CGACTACCTA
    GCGGCCGAAG AGAAGATCGT TATGTCCAAA AAGGAGTCTA TGTTCTCCTG GGATGAGACG
    GAGTACAAGA TCCCTGCGCG GCTCACCCTG CAGATCTGGG ACGCTGACCA CTTCTCGGCT
    GACGACTTCC TGGGGGCTAT CGAGCTGGAC CTGAACCGGT TCCCGAGGGG CGCTAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTACCCCT GGTTTCCATC
    TTTAAACAGA AACGTGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAATGATGAG
    TTTGAGCTCA CAGGCAAAGT GGAGGCGGAG CTACACCTAC TCACGGCAGA GGAGGCAGAG
    AAGAACCCTG TGGGCCTGGC TCGCAATGAA CCTGATCCCC TAGAAAAACC CAATCGGCCG
    GACACAAGCT TCATCTGGTT CTTGAACCCT CTCAAGTCTG CCCGCTACTT CCTGTGGCAT
    ACCTACCGCT GGCTACTCCT CAAATTCCTG CTGCTCTTCC TCCTGCTGCT GCTCTTCGCC
    CTGTTTCTCT ACTCTCTGCC TGGCTACCTG GCCAAGAAGA TCCTTGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_114081.2
    CDS135..6128
    Translation

    Target ORF information:

    RefSeq Version NM_031875.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus otoferlin (Otof), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_031875.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGGAC CTCCCGGAGA ATGAGCTCCT GCACCCACCC
    TTGAACATCC GAGTGGTAGA TTGCCGGGCC TTTGGACGAT ACACCCTGGT GGGTTCCCAC
    GCAGTCAGCT CACTGAGGCG CTTCATCTAC CGACCTCCAG ACCGCTCAGC CCCCAACTGG
    AACACCACAG TCAGGCTGCT CCGGGGCTGC CACAGGCTGC GCAATGGGGG CCCCTCTTCT
    CGCCCCACAG GGGAGGTTGT AGTAAGCATG GAGCCTGAGG AGCCAGTTAA GAAGCTGGAG
    ACCATGGTGA AACTGGATGC GACTTCTGAT GCTGTGGTCA AGGTGGATGT GGCTGAAGAT
    GAGAAGGAAA GGAAGAAGAA GAAAAAGAAA GGCCCGTCAG AGGAGCCAGA GGAGGAAGAG
    CCCGATGAGA GCATGCTGGA TTGGTGGTCC AAGTACTTCG CCTCCATCGA CACAATGAAG
    GAGCAACTTC GACAACATGA GACCTCTGGA ACTGACTTGG AAGAGAAGGA AGAGATGGAA
    AGCGCTGAGG GCCTGAAGGG ACCAATGAAG AGCAAGGAGA AGTCCAGAGC TGCAAAGGAG
    GAGAAAAAGA AGAAAAACCA GAGCCCTGGC CCTGGCCAGG GATCGGAGGC TCCTGAGAAG
    AAGAAAGCCA AGATCGATGA GCTTAAGGTG TACCCCAAGG AGCTGGAATC GGAGTTTGAC
    AGCTTTGAGG ACTGGCTGCA CACCTTCAAC CTGTTGAGGG GCAAGACGGG AGATGATGAG
    GATGGCTCCA CAGAGGAGGA GCGCATAGTA GGCCGATTCA AGGGCTCCCT CTGTGTGTAC
    AAAGTGCCAC TCCCAGAAGA TGTATCTCGA GAAGCTGGCT ATGATCCCAC CTATGGAATG
    TTCCAGGGCA TCCCAAGCAA TGACCCCATC AATGTGCTGG TCCGAATCTA TGTGGTCCGG
    GCCACAGACC TGCACCCGGC CGACATCAAT GGCAAAGCTG ACCCCTATAT TGCCATCAAG
    TTAGGCAAGA CCGACATCCG AGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTG
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AGTCCATGTT GACAGTGGCC
    GTGTACGACT GGGATCTGGT GGGCACTGAT GACCTCATCG GAGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCATCGCGCC ACCTGCGGCA TCGCACAGAC CTATTCCATA
    CATGGCTACA ATATCTGGAG GGACCCCATG AAGCCCAGCC AGATCCTGAC ACGCCTCTGT
    AAAGAGGGCA AAGTGGACGG CCCCCACTTT GGTCCCCATG GGAGAGTGAG GGTTGCCAAC
    CGTGTCTTCA CGGGGCCTTC AGAAATAGAG GATGAGAATG GTCAGAGGAA GCCCACAGAT
    GAGCACGTGG CACTGTCTGC TCTGAGACAC TGGGAGGACA TCCCCCGGGT GGGCTGCCGC
    CTTGTGCCGG AACACGTGGA GACCAGGCCG CTGCTCAACC CTGACAAGCC AGGCATTGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACA
    CCTCTGGATA TATCCCCCAG GAAACCCAAG AAGTACGAGC TGCGGGTCAT CGTGTGGAAC
    ACAGACGAGG TGGTCCTGGA AGACGATGAT TTCTTCACGG GAGAGAAGTC CAGTGACATT
    TTTGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAAC AGGACACAGA TGTCCACTAT
    CACTCCCTCA CGGGGGAGGG CAACTTCAAC TGGAGATACC TCTTCCCCTT CGACTACCTA
    GCGGCCGAAG AGAAGATCGT TATGTCCAAA AAGGAGTCTA TGTTCTCCTG GGATGAGACG
    GAGTACAAGA TCCCTGCGCG GCTCACCCTG CAGATCTGGG ACGCTGACCA CTTCTCGGCT
    GACGACTTCC TGGGGGCTAT CGAGCTGGAC CTGAACCGGT TCCCGAGGGG CGCTAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTACCCCT GGTTTCCATC
    TTTAAACAGA AACGTGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAATGATGAG
    TTTGAGCTCA CAGGCAAAGT GGAGGCGGAG CTACACCTAC TCACGGCAGA GGAGGCAGAG
    AAGAACCCTG TGGGCCTGGC TCGCAATGAA CCTGATCCCC TAGAAAAACC CAATCGGCCG
    GACACAAGCT TCATCTGGTT CTTGAACCCT CTCAAGTCTG CCCGCTACTT CCTGTGGCAT
    ACCTACCGCT GGCTACTCCT CAAATTCCTG CTGCTCTTCC TCCTGCTGCT GCTCTTCGCC
    CTGTTTCTCT ACTCTCTGCC TGGCTACCTG GCCAAGAAGA TCCTTGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu23304
    Clone ID Related Accession (Same CDS sequence) NM_001286421.1
    Accession Version NM_001286421.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5934bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Mus musculus(house mouse)
    Product otoferlin isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK046460.1, AK147256.1 and AK033317.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 2. It encodes isoform 3, which is shorter than isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150702.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGGAC CTCCCGGAGA ATGAGCTCCT GCACCCACCC
    TTGAACATCC GAGTGGTAGA TTGCCGGGCC TTTGGACGAT ACACCCTGGT GGGTTCCCAC
    GCAGTCAGCT CACTGAGGCG CTTCATCTAC CGACCTCCAG ACCGCTCAGC CCCCAACTGG
    AACACCACAG GGGAGGTTGT AGTAAGCATG GAGCCTGAGG AGCCAGTTAA GAAGCTGGAG
    ACCATGGTGA AACTGGATGC GACTTCTGAT GCTGTGGTCA AGGTGGATGT GGCTGAAGAT
    GAGAAGGAAA GGAAGAAGAA GAAAAAGAAA GGCCCGTCAG AGGAGCCAGA GGAGGAAGAG
    CCCGATGAGA GCATGCTGGA TTGGTGGTCC AAGTACTTCG CCTCCATCGA CACAATGAAG
    GAGCAACTTC GACAACATGA GACCTCTGGA ACTGACTTGG AAGAGAAGGA AGAGATGGAA
    AGCGCTGAGG GCCTGAAGGG ACCAATGAAG AGCAAGGAGA AGTCCAGAGC TGCAAAGGAG
    GAGAAAAAGA AGAAAAACCA GAGCCCTGGC CCTGGCCAGG GATCGGAGGC TCCTGAGAAG
    AAGAAAGCCA AGATCGATGA GCTTAAGGTG TACCCCAAGG AGCTGGAATC GGAGTTTGAC
    AGCTTTGAGG ACTGGCTGCA CACCTTCAAC CTGTTGAGGG GCAAGACGGG AGATGATGAG
    GATGGCTCCA CAGAGGAGGA GCGCATAGTA GGCCGATTCA AGGGCTCCCT CTGTGTGTAC
    AAAGTGCCAC TCCCAGAAGA TGTATCTCGA GAAGCTGGCT ATGATCCCAC CTATGGAATG
    TTCCAGGGCA TCCCAAGCAA TGACCCCATC AATGTGCTGG TCCGAATCTA TGTGGTCCGG
    GCCACAGACC TGCACCCGGC CGACATCAAT GGCAAAGCTG ACCCCTATAT TGCCATCAAG
    TTAGGCAAGA CCGACATCCG AGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTG
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AGTCCATGTT GACAGTGGCC
    GTGTACGACT GGGATCTGGT GGGCACTGAT GACCTCATCG GAGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCATCGCGCC ACCTGCGGCA TCGCACAGAC CTATTCCATA
    CATGGCTACA ATATCTGGAG GGACCCCATG AAGCCCAGCC AGATCCTGAC ACGCCTCTGT
    AAAGAGGGCA AAGTGGACGG CCCCCACTTT GGTCCCCATG GGAGAGTGAG GGTTGCCAAC
    CGTGTCTTCA CGGGGCCTTC AGAAATAGAG GATGAGAATG GTCAGAGGAA GCCCACAGAT
    GAGCACGTGG CACTGTCTGC TCTGAGACAC TGGGAGGACA TCCCCCGGGT GGGCTGCCGC
    CTTGTGCCGG AACACGTGGA GACCAGGCCG CTGCTCAACC CTGACAAGCC AGGCATTGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACA
    CCTCTGGATA TATCCCCCAG GAAACCCAAG AAGTACGAGC TGCGGGTCAT CGTGTGGAAC
    ACAGACGAGG TGGTCCTGGA AGACGATGAT TTCTTCACGG GAGAGAAGTC CAGTGACATT
    TTTGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAAC AGGACACAGA TGTCCACTAT
    CACTCCCTCA CGGGGGAGGG CAACTTCAAC TGGAGATACC TCTTCCCCTT CGACTACCTA
    GCGGCCGAAG AGAAGATCGT TATGTCCAAA AAGGAGTCTA TGTTCTCCTG GGATGAGACG
    GAGTACAAGA TCCCTGCGCG GCTCACCCTG CAGATCTGGG ACGCTGACCA CTTCTCGGCT
    GACGACTTCC TGGGGGCTAT CGAGCTGGAC CTGAACCGGT TCCCGAGGGG CGCTAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTACCCCT GGTTTCCATC
    TTTAAACAGA AACGTGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAATGATGAG
    TTTGAGCTCA CAGGCAAAGT GGAGGCGGAG CTACACCTAC TCACGGCAGA GGAGGCAGAG
    AAGAACCCTG TGGGCCTGGC TCGCAATGAA CCTGATCCCC TAGAAAAACC CAATCGGCCG
    GACACAAGCT TCATCTGGTT CTTGAACCCT CTCAAGTCTG CCCGCTACTT CCTGTGGCAT
    ACCTACCGCT GGCTACTCCT CAAATTCCTG CTGCTCTTCC TCCTGCTGCT GCTCTTCGCC
    CTGTTTCTCT ACTCTCTGCC TGGCTACCTG GCCAAGAAGA TCCTTGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001273350.1
    CDS135..6068
    Translation

    Target ORF information:

    RefSeq Version NM_001286421.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus otoferlin (Otof), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001286421.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGGAC CTCCCGGAGA ATGAGCTCCT GCACCCACCC
    TTGAACATCC GAGTGGTAGA TTGCCGGGCC TTTGGACGAT ACACCCTGGT GGGTTCCCAC
    GCAGTCAGCT CACTGAGGCG CTTCATCTAC CGACCTCCAG ACCGCTCAGC CCCCAACTGG
    AACACCACAG GGGAGGTTGT AGTAAGCATG GAGCCTGAGG AGCCAGTTAA GAAGCTGGAG
    ACCATGGTGA AACTGGATGC GACTTCTGAT GCTGTGGTCA AGGTGGATGT GGCTGAAGAT
    GAGAAGGAAA GGAAGAAGAA GAAAAAGAAA GGCCCGTCAG AGGAGCCAGA GGAGGAAGAG
    CCCGATGAGA GCATGCTGGA TTGGTGGTCC AAGTACTTCG CCTCCATCGA CACAATGAAG
    GAGCAACTTC GACAACATGA GACCTCTGGA ACTGACTTGG AAGAGAAGGA AGAGATGGAA
    AGCGCTGAGG GCCTGAAGGG ACCAATGAAG AGCAAGGAGA AGTCCAGAGC TGCAAAGGAG
    GAGAAAAAGA AGAAAAACCA GAGCCCTGGC CCTGGCCAGG GATCGGAGGC TCCTGAGAAG
    AAGAAAGCCA AGATCGATGA GCTTAAGGTG TACCCCAAGG AGCTGGAATC GGAGTTTGAC
    AGCTTTGAGG ACTGGCTGCA CACCTTCAAC CTGTTGAGGG GCAAGACGGG AGATGATGAG
    GATGGCTCCA CAGAGGAGGA GCGCATAGTA GGCCGATTCA AGGGCTCCCT CTGTGTGTAC
    AAAGTGCCAC TCCCAGAAGA TGTATCTCGA GAAGCTGGCT ATGATCCCAC CTATGGAATG
    TTCCAGGGCA TCCCAAGCAA TGACCCCATC AATGTGCTGG TCCGAATCTA TGTGGTCCGG
    GCCACAGACC TGCACCCGGC CGACATCAAT GGCAAAGCTG ACCCCTATAT TGCCATCAAG
    TTAGGCAAGA CCGACATCCG AGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTG
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AGTCCATGTT GACAGTGGCC
    GTGTACGACT GGGATCTGGT GGGCACTGAT GACCTCATCG GAGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCATCGCGCC ACCTGCGGCA TCGCACAGAC CTATTCCATA
    CATGGCTACA ATATCTGGAG GGACCCCATG AAGCCCAGCC AGATCCTGAC ACGCCTCTGT
    AAAGAGGGCA AAGTGGACGG CCCCCACTTT GGTCCCCATG GGAGAGTGAG GGTTGCCAAC
    CGTGTCTTCA CGGGGCCTTC AGAAATAGAG GATGAGAATG GTCAGAGGAA GCCCACAGAT
    GAGCACGTGG CACTGTCTGC TCTGAGACAC TGGGAGGACA TCCCCCGGGT GGGCTGCCGC
    CTTGTGCCGG AACACGTGGA GACCAGGCCG CTGCTCAACC CTGACAAGCC AGGCATTGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACA
    CCTCTGGATA TATCCCCCAG GAAACCCAAG AAGTACGAGC TGCGGGTCAT CGTGTGGAAC
    ACAGACGAGG TGGTCCTGGA AGACGATGAT TTCTTCACGG GAGAGAAGTC CAGTGACATT
    TTTGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAAC AGGACACAGA TGTCCACTAT
    CACTCCCTCA CGGGGGAGGG CAACTTCAAC TGGAGATACC TCTTCCCCTT CGACTACCTA
    GCGGCCGAAG AGAAGATCGT TATGTCCAAA AAGGAGTCTA TGTTCTCCTG GGATGAGACG
    GAGTACAAGA TCCCTGCGCG GCTCACCCTG CAGATCTGGG ACGCTGACCA CTTCTCGGCT
    GACGACTTCC TGGGGGCTAT CGAGCTGGAC CTGAACCGGT TCCCGAGGGG CGCTAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTACCCCT GGTTTCCATC
    TTTAAACAGA AACGTGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAATGATGAG
    TTTGAGCTCA CAGGCAAAGT GGAGGCGGAG CTACACCTAC TCACGGCAGA GGAGGCAGAG
    AAGAACCCTG TGGGCCTGGC TCGCAATGAA CCTGATCCCC TAGAAAAACC CAATCGGCCG
    GACACAAGCT TCATCTGGTT CTTGAACCCT CTCAAGTCTG CCCGCTACTT CCTGTGGCAT
    ACCTACCGCT GGCTACTCCT CAAATTCCTG CTGCTCTTCC TCCTGCTGCT GCTCTTCGCC
    CTGTTTCTCT ACTCTCTGCC TGGCTACCTG GCCAAGAAGA TCCTTGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16389
    Clone ID Related Accession (Same CDS sequence) NM_031875.2 , XM_017321185.1
    Accession Version XM_017321185.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product otoferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGGAC CTCCCGGAGA ATGAGCTCCT GCACCCACCC
    TTGAACATCC GAGTGGTAGA TTGCCGGGCC TTTGGACGAT ACACCCTGGT GGGTTCCCAC
    GCAGTCAGCT CACTGAGGCG CTTCATCTAC CGACCTCCAG ACCGCTCAGC CCCCAACTGG
    AACACCACAG TCAGGCTGCT CCGGGGCTGC CACAGGCTGC GCAATGGGGG CCCCTCTTCT
    CGCCCCACAG GGGAGGTTGT AGTAAGCATG GAGCCTGAGG AGCCAGTTAA GAAGCTGGAG
    ACCATGGTGA AACTGGATGC GACTTCTGAT GCTGTGGTCA AGGTGGATGT GGCTGAAGAT
    GAGAAGGAAA GGAAGAAGAA GAAAAAGAAA GGCCCGTCAG AGGAGCCAGA GGAGGAAGAG
    CCCGATGAGA GCATGCTGGA TTGGTGGTCC AAGTACTTCG CCTCCATCGA CACAATGAAG
    GAGCAACTTC GACAACATGA GACCTCTGGA ACTGACTTGG AAGAGAAGGA AGAGATGGAA
    AGCGCTGAGG GCCTGAAGGG ACCAATGAAG AGCAAGGAGA AGTCCAGAGC TGCAAAGGAG
    GAGAAAAAGA AGAAAAACCA GAGCCCTGGC CCTGGCCAGG GATCGGAGGC TCCTGAGAAG
    AAGAAAGCCA AGATCGATGA GCTTAAGGTG TACCCCAAGG AGCTGGAATC GGAGTTTGAC
    AGCTTTGAGG ACTGGCTGCA CACCTTCAAC CTGTTGAGGG GCAAGACGGG AGATGATGAG
    GATGGCTCCA CAGAGGAGGA GCGCATAGTA GGCCGATTCA AGGGCTCCCT CTGTGTGTAC
    AAAGTGCCAC TCCCAGAAGA TGTATCTCGA GAAGCTGGCT ATGATCCCAC CTATGGAATG
    TTCCAGGGCA TCCCAAGCAA TGACCCCATC AATGTGCTGG TCCGAATCTA TGTGGTCCGG
    GCCACAGACC TGCACCCGGC CGACATCAAT GGCAAAGCTG ACCCCTATAT TGCCATCAAG
    TTAGGCAAGA CCGACATCCG AGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTG
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AGTCCATGTT GACAGTGGCC
    GTGTACGACT GGGATCTGGT GGGCACTGAT GACCTCATCG GAGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCATCGCGCC ACCTGCGGCA TCGCACAGAC CTATTCCATA
    CATGGCTACA ATATCTGGAG GGACCCCATG AAGCCCAGCC AGATCCTGAC ACGCCTCTGT
    AAAGAGGGCA AAGTGGACGG CCCCCACTTT GGTCCCCATG GGAGAGTGAG GGTTGCCAAC
    CGTGTCTTCA CGGGGCCTTC AGAAATAGAG GATGAGAATG GTCAGAGGAA GCCCACAGAT
    GAGCACGTGG CACTGTCTGC TCTGAGACAC TGGGAGGACA TCCCCCGGGT GGGCTGCCGC
    CTTGTGCCGG AACACGTGGA GACCAGGCCG CTGCTCAACC CTGACAAGCC AGGCATTGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACA
    CCTCTGGATA TATCCCCCAG GAAACCCAAG AAGTACGAGC TGCGGGTCAT CGTGTGGAAC
    ACAGACGAGG TGGTCCTGGA AGACGATGAT TTCTTCACGG GAGAGAAGTC CAGTGACATT
    TTTGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAAC AGGACACAGA TGTCCACTAT
    CACTCCCTCA CGGGGGAGGG CAACTTCAAC TGGAGATACC TCTTCCCCTT CGACTACCTA
    GCGGCCGAAG AGAAGATCGT TATGTCCAAA AAGGAGTCTA TGTTCTCCTG GGATGAGACG
    GAGTACAAGA TCCCTGCGCG GCTCACCCTG CAGATCTGGG ACGCTGACCA CTTCTCGGCT
    GACGACTTCC TGGGGGCTAT CGAGCTGGAC CTGAACCGGT TCCCGAGGGG CGCTAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTACCCCT GGTTTCCATC
    TTTAAACAGA AACGTGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAATGATGAG
    TTTGAGCTCA CAGGCAAAGT GGAGGCGGAG CTACACCTAC TCACGGCAGA GGAGGCAGAG
    AAGAACCCTG TGGGCCTGGC TCGCAATGAA CCTGATCCCC TAGAAAAACC CAATCGGCCG
    GACACAAGCT TCATCTGGTT CTTGAACCCT CTCAAGTCTG CCCGCTACTT CCTGTGGCAT
    ACCTACCGCT GGCTACTCCT CAAATTCCTG CTGCTCTTCC TCCTGCTGCT GCTCTTCGCC
    CTGTTTCTCT ACTCTCTGCC TGGCTACCTG GCCAAGAAGA TCCTTGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176674.1
    CDS903..6896
    Translation

    Target ORF information:

    RefSeq Version XM_017321185.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus otoferlin (Otof), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321185.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGGAC CTCCCGGAGA ATGAGCTCCT GCACCCACCC
    TTGAACATCC GAGTGGTAGA TTGCCGGGCC TTTGGACGAT ACACCCTGGT GGGTTCCCAC
    GCAGTCAGCT CACTGAGGCG CTTCATCTAC CGACCTCCAG ACCGCTCAGC CCCCAACTGG
    AACACCACAG TCAGGCTGCT CCGGGGCTGC CACAGGCTGC GCAATGGGGG CCCCTCTTCT
    CGCCCCACAG GGGAGGTTGT AGTAAGCATG GAGCCTGAGG AGCCAGTTAA GAAGCTGGAG
    ACCATGGTGA AACTGGATGC GACTTCTGAT GCTGTGGTCA AGGTGGATGT GGCTGAAGAT
    GAGAAGGAAA GGAAGAAGAA GAAAAAGAAA GGCCCGTCAG AGGAGCCAGA GGAGGAAGAG
    CCCGATGAGA GCATGCTGGA TTGGTGGTCC AAGTACTTCG CCTCCATCGA CACAATGAAG
    GAGCAACTTC GACAACATGA GACCTCTGGA ACTGACTTGG AAGAGAAGGA AGAGATGGAA
    AGCGCTGAGG GCCTGAAGGG ACCAATGAAG AGCAAGGAGA AGTCCAGAGC TGCAAAGGAG
    GAGAAAAAGA AGAAAAACCA GAGCCCTGGC CCTGGCCAGG GATCGGAGGC TCCTGAGAAG
    AAGAAAGCCA AGATCGATGA GCTTAAGGTG TACCCCAAGG AGCTGGAATC GGAGTTTGAC
    AGCTTTGAGG ACTGGCTGCA CACCTTCAAC CTGTTGAGGG GCAAGACGGG AGATGATGAG
    GATGGCTCCA CAGAGGAGGA GCGCATAGTA GGCCGATTCA AGGGCTCCCT CTGTGTGTAC
    AAAGTGCCAC TCCCAGAAGA TGTATCTCGA GAAGCTGGCT ATGATCCCAC CTATGGAATG
    TTCCAGGGCA TCCCAAGCAA TGACCCCATC AATGTGCTGG TCCGAATCTA TGTGGTCCGG
    GCCACAGACC TGCACCCGGC CGACATCAAT GGCAAAGCTG ACCCCTATAT TGCCATCAAG
    TTAGGCAAGA CCGACATCCG AGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTG
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AGTCCATGTT GACAGTGGCC
    GTGTACGACT GGGATCTGGT GGGCACTGAT GACCTCATCG GAGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCATCGCGCC ACCTGCGGCA TCGCACAGAC CTATTCCATA
    CATGGCTACA ATATCTGGAG GGACCCCATG AAGCCCAGCC AGATCCTGAC ACGCCTCTGT
    AAAGAGGGCA AAGTGGACGG CCCCCACTTT GGTCCCCATG GGAGAGTGAG GGTTGCCAAC
    CGTGTCTTCA CGGGGCCTTC AGAAATAGAG GATGAGAATG GTCAGAGGAA GCCCACAGAT
    GAGCACGTGG CACTGTCTGC TCTGAGACAC TGGGAGGACA TCCCCCGGGT GGGCTGCCGC
    CTTGTGCCGG AACACGTGGA GACCAGGCCG CTGCTCAACC CTGACAAGCC AGGCATTGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACA
    CCTCTGGATA TATCCCCCAG GAAACCCAAG AAGTACGAGC TGCGGGTCAT CGTGTGGAAC
    ACAGACGAGG TGGTCCTGGA AGACGATGAT TTCTTCACGG GAGAGAAGTC CAGTGACATT
    TTTGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAAC AGGACACAGA TGTCCACTAT
    CACTCCCTCA CGGGGGAGGG CAACTTCAAC TGGAGATACC TCTTCCCCTT CGACTACCTA
    GCGGCCGAAG AGAAGATCGT TATGTCCAAA AAGGAGTCTA TGTTCTCCTG GGATGAGACG
    GAGTACAAGA TCCCTGCGCG GCTCACCCTG CAGATCTGGG ACGCTGACCA CTTCTCGGCT
    GACGACTTCC TGGGGGCTAT CGAGCTGGAC CTGAACCGGT TCCCGAGGGG CGCTAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTACCCCT GGTTTCCATC
    TTTAAACAGA AACGTGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAATGATGAG
    TTTGAGCTCA CAGGCAAAGT GGAGGCGGAG CTACACCTAC TCACGGCAGA GGAGGCAGAG
    AAGAACCCTG TGGGCCTGGC TCGCAATGAA CCTGATCCCC TAGAAAAACC CAATCGGCCG
    GACACAAGCT TCATCTGGTT CTTGAACCCT CTCAAGTCTG CCCGCTACTT CCTGTGGCAT
    ACCTACCGCT GGCTACTCCT CAAATTCCTG CTGCTCTTCC TCCTGCTGCT GCTCTTCGCC
    CTGTTTCTCT ACTCTCTGCC TGGCTACCTG GCCAAGAAGA TCCTTGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35274
    Clone ID Related Accession (Same CDS sequence) XM_006504206.3
    Accession Version XM_006504206.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product otoferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504206.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGGAC CTCCCGGAGA ATGAGCTCCT GCACCCACCC
    TTGAACATCC GAGTGGTAGA TTGCCGGGCC TTTGGACGAT ACACCCTGGT GGGTTCCCAC
    GCAGTCAGCT CACTGAGGCG CTTCATCTAC CGACCTCCAG ACCGCTCAGC CCCCAACTGG
    AACACCACAG TCAGGCTGCT CCGGGGCTGC CACAGGCTGC GCAATGGGGG CCCCTCTTCT
    CGCCCCACAG GGGAGGTTGT AGTAAGCATG GAGCCTGAGG AGCCAGTTAA GAAGCTGGAG
    ACCATGGTGA AACTGGATGC GACTTCTGAT GCTGTGGTCA AGGTGGATGT GGCTGAAGAT
    GAGAAGGAAA GGAAGAAGAA GAAAAAGAAA GGCCCGTCAG AGGAGCCAGA GGAGGAAGAG
    CCCGATGAGA GCATGCTGGA TTGGTGGTCC AAGTACTTCG CCTCCATCGA CACAATGAAG
    GAGCAACTTC GACAACATGA GACCTCTGGA ACTGACTTGG AAGAGAAGGA AGAGATGGAA
    AGCGCTGAGG GCCTGAAGGG ACCAATGAAG AGCAAGGAGA AGTCCAGAGC TGCAAAGGAG
    GAGAAAAAGA AGAAAAACCA GAGCCCTGGC CCTGGCCAGG GATCGGAGGC TCCTGAGAAG
    AAGAAAGCCA AGATCGATGA GCTTAAGGTG TACCCCAAGG AGCTGGAATC GGAGTTTGAC
    AGCTTTGAGG ACTGGCTGCA CACCTTCAAC CTGTTGAGGG GCAAGACGGG AGATGATGAG
    GATGGCTCCA CAGAGGAGGA GCGCATAGTA GGCCGATTCA AGGGCTCCCT CTGTGTGTAC
    AAAGTGCCAC TCCCAGAAGA TGTATCTCGA GAAGCTGGCT ATGATCCCAC CTATGGAATG
    TTCCAGGGCA TCCCAAGCAA TGACCCCATC AATGTGCTGG TCCGAATCTA TGTGGTCCGG
    GCCACAGACC TGCACCCGGC CGACATCAAT GGCAAAGCTG ACCCCTATAT TGCCATCAAG
    TTAGGCAAGA CCGACATCCG AGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTG
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AGTCCATGTT GACAGTGGCC
    GTGTACGACT GGGATCTGGT GGGCACTGAT GACCTCATCG GAGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCATCGCGCC ACCTGCGGCA TCGCACAGAC CTATTCCATA
    CATGGCTACA ATATCTGGAG GGACCCCATG AAGCCCAGCC AGATCCTGAC ACGCCTCTGT
    AAAGAGGGCA AAGTGGACGG CCCCCACTTT GGTCCCCATG GGAGAGTGAG GGTTGCCAAC
    CGTGTCTTCA CGGGGCCTTC AGAAATAGAG GATGAGAATG GTCAGAGGAA GCCCACAGAT
    GAGCACGTGG CACTGTCTGC TCTGAGACAC TGGGAGGACA TCCCCCGGGT GGGCTGCCGC
    CTTGTGCCGG AACACGTGGA GACCAGGCCG CTGCTCAACC CTGACAAGCC AGGCATTGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACA
    CCTCTGGATA TATCCCCCAG GAAACCCAAG AAGTACGAGC TGCGGGTCAT CGTGTGGAAC
    ACAGACGAGG TGGTCCTGGA AGACGATGAT TTCTTCACGG GAGAGAAGTC CAGTGACATT
    TTTGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAAC AGGACACAGA TGTCCACTAT
    CACTCCCTCA CGGGGGAGGG CAACTTCAAC TGGAGATACC TCTTCCCCTT CGACTACCTA
    GCGGCCGAAG AGAAGATCGT TATGTCCAAA AAGGAGTCTA TGTTCTCCTG GGATGAGACG
    GAGTACAAGA TCCCTGCGCG GCTCACCCTG CAGATCTGGG ACGCTGACCA CTTCTCGGCT
    GACGACTTCC TGGGGGCTAT CGAGCTGGAC CTGAACCGGT TCCCGAGGGG CGCTAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTACCCCT GGTTTCCATC
    TTTAAACAGA AACGTGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAATGATGAG
    TTTGAGCTCA CAGGCAAAGT GGAGGCGGAG CTACACCTAC TCACGGCAGA GGAGGCAGAG
    AAGAACCCTG TGGGCCTGGC TCGCAATGAA CCTGATCCCC TAGAAAAACC CAACCGGCCT
    GACACGGCAT TCGTCTGGTT CCTGAACCCA CTCAAATCTA TCAAGTACCT CATCTGCACC
    CGGTACAAGT GGCTGATCAT CAAGATCGTG CTGGCGCTGC TGGGGCTGCT CATGCTGGCC
    CTCTTCCTTT ACAGCCTCCC AGGCTACATG GTCAAGAAGC TCCTAGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006504269.1
    CDS906..6899
    Translation

    Target ORF information:

    RefSeq Version XM_006504206.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus otoferlin (Otof), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006504206.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGGAC CTCCCGGAGA ATGAGCTCCT GCACCCACCC
    TTGAACATCC GAGTGGTAGA TTGCCGGGCC TTTGGACGAT ACACCCTGGT GGGTTCCCAC
    GCAGTCAGCT CACTGAGGCG CTTCATCTAC CGACCTCCAG ACCGCTCAGC CCCCAACTGG
    AACACCACAG TCAGGCTGCT CCGGGGCTGC CACAGGCTGC GCAATGGGGG CCCCTCTTCT
    CGCCCCACAG GGGAGGTTGT AGTAAGCATG GAGCCTGAGG AGCCAGTTAA GAAGCTGGAG
    ACCATGGTGA AACTGGATGC GACTTCTGAT GCTGTGGTCA AGGTGGATGT GGCTGAAGAT
    GAGAAGGAAA GGAAGAAGAA GAAAAAGAAA GGCCCGTCAG AGGAGCCAGA GGAGGAAGAG
    CCCGATGAGA GCATGCTGGA TTGGTGGTCC AAGTACTTCG CCTCCATCGA CACAATGAAG
    GAGCAACTTC GACAACATGA GACCTCTGGA ACTGACTTGG AAGAGAAGGA AGAGATGGAA
    AGCGCTGAGG GCCTGAAGGG ACCAATGAAG AGCAAGGAGA AGTCCAGAGC TGCAAAGGAG
    GAGAAAAAGA AGAAAAACCA GAGCCCTGGC CCTGGCCAGG GATCGGAGGC TCCTGAGAAG
    AAGAAAGCCA AGATCGATGA GCTTAAGGTG TACCCCAAGG AGCTGGAATC GGAGTTTGAC
    AGCTTTGAGG ACTGGCTGCA CACCTTCAAC CTGTTGAGGG GCAAGACGGG AGATGATGAG
    GATGGCTCCA CAGAGGAGGA GCGCATAGTA GGCCGATTCA AGGGCTCCCT CTGTGTGTAC
    AAAGTGCCAC TCCCAGAAGA TGTATCTCGA GAAGCTGGCT ATGATCCCAC CTATGGAATG
    TTCCAGGGCA TCCCAAGCAA TGACCCCATC AATGTGCTGG TCCGAATCTA TGTGGTCCGG
    GCCACAGACC TGCACCCGGC CGACATCAAT GGCAAAGCTG ACCCCTATAT TGCCATCAAG
    TTAGGCAAGA CCGACATCCG AGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTG
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AGTCCATGTT GACAGTGGCC
    GTGTACGACT GGGATCTGGT GGGCACTGAT GACCTCATCG GAGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCATCGCGCC ACCTGCGGCA TCGCACAGAC CTATTCCATA
    CATGGCTACA ATATCTGGAG GGACCCCATG AAGCCCAGCC AGATCCTGAC ACGCCTCTGT
    AAAGAGGGCA AAGTGGACGG CCCCCACTTT GGTCCCCATG GGAGAGTGAG GGTTGCCAAC
    CGTGTCTTCA CGGGGCCTTC AGAAATAGAG GATGAGAATG GTCAGAGGAA GCCCACAGAT
    GAGCACGTGG CACTGTCTGC TCTGAGACAC TGGGAGGACA TCCCCCGGGT GGGCTGCCGC
    CTTGTGCCGG AACACGTGGA GACCAGGCCG CTGCTCAACC CTGACAAGCC AGGCATTGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACA
    CCTCTGGATA TATCCCCCAG GAAACCCAAG AAGTACGAGC TGCGGGTCAT CGTGTGGAAC
    ACAGACGAGG TGGTCCTGGA AGACGATGAT TTCTTCACGG GAGAGAAGTC CAGTGACATT
    TTTGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAAC AGGACACAGA TGTCCACTAT
    CACTCCCTCA CGGGGGAGGG CAACTTCAAC TGGAGATACC TCTTCCCCTT CGACTACCTA
    GCGGCCGAAG AGAAGATCGT TATGTCCAAA AAGGAGTCTA TGTTCTCCTG GGATGAGACG
    GAGTACAAGA TCCCTGCGCG GCTCACCCTG CAGATCTGGG ACGCTGACCA CTTCTCGGCT
    GACGACTTCC TGGGGGCTAT CGAGCTGGAC CTGAACCGGT TCCCGAGGGG CGCTAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTACCCCT GGTTTCCATC
    TTTAAACAGA AACGTGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAATGATGAG
    TTTGAGCTCA CAGGCAAAGT GGAGGCGGAG CTACACCTAC TCACGGCAGA GGAGGCAGAG
    AAGAACCCTG TGGGCCTGGC TCGCAATGAA CCTGATCCCC TAGAAAAACC CAACCGGCCT
    GACACGGCAT TCGTCTGGTT CCTGAACCCA CTCAAATCTA TCAAGTACCT CATCTGCACC
    CGGTACAAGT GGCTGATCAT CAAGATCGTG CTGGCGCTGC TGGGGCTGCT CATGCTGGCC
    CTCTTCCTTT ACAGCCTCCC AGGCTACATG GTCAAGAAGC TCCTAGGGGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35276
    Clone ID Related Accession (Same CDS sequence) XM_006504208.3 , XM_006504208.4
    Accession Version XM_006504208.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5820bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product otoferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504208.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGGAC CTCCCGGAGA ATGAGCTCCT GCACCCACCC
    TTGAACATCC GAGTGGTAGA TTGCCGGGCC TTTGGACGAT ACACCCTGGT GGGTTCCCAC
    GCAGTCAGCT CACTGAGGCG CTTCATCTAC CGACCTCCAG ACCGCTCAGC CCCCAACTGG
    AACACCACAG TCAGGCTGCT CCGGGGCTGC CACAGGCTGC GCAATGGGGG CCCCTCTTCT
    CGCCCCACAG GGGAGGTTGT AGTAAGCATG GAGCCTGAGG AGCCAGTTAA GAAGCTGGAG
    ACCATGGTGA AACTGGATGC GACTTCTGAT GCTGTGGTCA AGGTGGATGT GGCTGAAGAT
    GAGAAGGAAA GGAAGAAGAA GAAAAAGAAA GGCCCGTCAG AGGAGCCAGA GGAGGAAGAG
    CCCGATGAGA GCATGCTGGA TTGGTGGTCC AAGTACTTCG CCTCCATCGA CACAATGAAG
    GAGCAACTTC GACAACATGA GACCTCTGGA ACTGACTTGG AAGAGAAGGA AGAGATGGAA
    AGCGCTGAGG GCCTGAAGGG ACCAATGAAG AGCAAGGAGA AGTCCAGAGC TGCAAAGGAG
    GAGAAAAAGA AGAAAAACCA GAGCCCTGGC CCTGGCCAGG GATCGGAGGC TCCTGAGAAG
    AAGAAAGCCA AGATCGATGA GCTTAAGGTG TACCCCAAGG AGCTGGAATC GGAGTTTGAC
    AGCTTTGAGG ACTGGCTGCA CACCTTCAAC CTGTTGAGGG GCAAGACGGG AGATGATGAG
    GATGGCTCCA CAGAGGAGGA GCGCATAGTA GGCCGATTCA AGGGCTCCCT CTGTGTGTAC
    AAAGTGCCAC TCCCAGAAGA TGTATCTCGA GAAGCTGGCT ATGATCCCAC CTATGGAATG
    TTCCAGGGCA TCCCAAGCAA TGACCCCATC AATGTGCTGG TCCGAATCTA TGTGGTCCGG
    GCCACAGACC TGCACCCGGC CGACATCAAT GGCAAAGCTG ACCCCTATAT TGCCATCAAG
    TTAGGCAAGA CCGACATCCG AGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTG
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AGTCCATGTT GACAGTGGCC
    GTGTACGACT GGGATCTGGT GGGCACTGAT GACCTCATCG GAGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCATCGCGCC ACCTGCGGCA TCGCACAGAC CTATTCCATA
    CATGGCTACA ATATCTGGAG GGACCCCATG AAGCCCAGCC AGATCCTGAC ACGCCTCTGT
    AAAGAGGGCA AAGTGGACGG CCCCCACTTT GGTCCCCATG GGAGAGTGAG GGTTGCCAAC
    CGTGTCTTCA CGGGGCCTTC AGAAATAGAG GATGAGAATG GTCAGAGGAA GCCCACAGAT
    GAGCACGTGG CACTGTCTGC TCTGAGACAC TGGGAGGACA TCCCCCGGGT GGGCTGCCGC
    CTTGTGCCGG AACACGTGGA GACCAGGCCG CTGCTCAACC CTGACAAGCC AGGCATTGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACA
    CCTCTGGATA TATCCCCCAG GAAACCCAAG AAGTACGAGC TGCGGGTCAT CGTGTGGAAC
    ACAGACGAGG TGGTCCTGGA AGACGATGAT TTCTTCACGG GAGAGAAGTC CAGTGACATT
    TTTGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAAC AGGACACAGA TGTCCACTAT
    CACTCCCTCA CGGGGGAGGG CAACTTCAAC TGGAGATACC TCTTCCCCTT CGACTACCTA
    GCGGCCGAAG AGAAGATCGT TATGTCCAAA AAGGAGTCTA TGTTCTCCTG GGATGAGACG
    GAGTACAAGA TCCCTGCGCG GCTCACCCTG CAGATCTGGG ACGCTGACCA CTTCTCGGCT
    GACGACTTCC TGGGGGCTAT CGAGCTGGAC CTGAACCGGT TCCCGAGGGG CGCTAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTACCCCT GGTTTCCATC
    TTTAAACAGA AACGTGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAATGATGAG
    TTTGAGCTCA CAGGCAAAGT GGAGGCGGAG CTACACCTAC TCACGGCAGA GGAGGCAGAG
    AAGAACCCTG TGGGCCTGGC TCGCAATGAA CCTGATCCCC TAGAAAAACC CAATTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006504271.1
    CDS906..6725
    Translation

    Target ORF information:

    RefSeq Version XM_006504208.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus otoferlin (Otof), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006504208.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGCCCTGA TTGTTCACCT CAAGACTGTC TCAGAGCTCC GAGGCAAAGG TGACCGGATT 
    GCCAAAGTCA CTTTCCGAGG GCAGTCTTTC TACTCCCGGG TCCTGGAGAA CTGCGAGGGT
    GTGGCTGACT TTGATGAGAC GTTCCGGTGG CCAGTGGCCA GCAGCATCGA CCGGAATGAA
    GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT GATAGGGACC
    TTCTGCATGG TGCTGCAGAA AGTGGTGGAG GAGAATCGGG TAGAGGTGAC CGACACGCTG
    ATGGATGACA GCAATGCTAT CATCAAGACC AGCCTGAGCA TGGAGGTCCG GTATCAGGCC
    ACAGATGGCA CTGTGGGCCC CTGGGATGAT GGAGACTTCC TGGGAGATGA ATCCCTCCAG
    GAGGAGAAGG ACAGCCAGGA GACAGATGGG CTGCTACCTG GTTCCCGACC CAGCACCCGG
    ATATCTGGCG AGAAGAGCTT TCGCAGAGCG GGAAGGAGTG TGTTCTCGGC CATGAAACTC
    GGCAAAACTC GGTCCCACAA AGAGGAGCCC CAAAGACAAG ATGAGCCAGC AGTGCTGGAG
    ATGGAGGACC TGGACCACCT AGCCATTCAG CTGGGGGATG GGCTGGATCC TGACTCCGTG
    TCTCTAGCCT CGGTCACCGC TCTCACCAGC AATGTCTCCA ACAAACGGTC TAAGCCAGAT
    ATTAAGATGG AGCCCAGTGC TGGAAGGCCC ATGGATTACC AGGTCAGCAT CACAGTGATT
    GAGGCTCGGC AGCTGGTGGG CTTGAACATG GACCCTGTGG TGTGTGTGGA GGTGGGTGAT
    GACAAGAAAT ACACGTCAAT GAAGGAGTCC ACAAACTGCC CTTACTACAA CGAGTACTTT
    GTCTTCGACT TCCATGTCTC TCCTGATGTC ATGTTTGACA AGATCATCAA GATCTCGGTT
    ATCCATTCTA AGAACCTGCT TCGGAGCGGC ACCCTGGTGG GTTCCTTCAA AATGGATGTG
    GGGACTGTGT ATTCCCAGCC TGAACACCAG TTCCATCACA AATGGGCCAT CCTGTCAGAC
    CCCGATGACA TCTCTGCTGG GTTGAAGGGT TATGTAAAGT GTGATGTCGC TGTGGTGGGC
    AAGGGAGACA ACATCAAGAC ACCCCACAAG GCCAACGAGA CGGATGAGGA CGACATTGAA
    GGGAACTTGC TGCTCCCCGA GGGCGTGCCC CCCGAACGGC AGTGGGCACG GTTCTATGTG
    AAAATTTACC GAGCAGAGGG ACTGCCCCGG ATGAACACAA GCCTCATGGC CAACGTGAAG
    AAGGCGTTCA TCGGTGAGAA CAAGGACCTC GTCGACCCCT ATGTGCAAGT CTTCTTTGCT
    GGACAAAAGG GCAAAACATC AGTGCAGAAG AGCAGCTATG AGCCGCTATG GAATGAGCAG
    GTCGTCTTCA CAGACTTGTT CCCCCCACTC TGCAAACGCA TGAAGGTGCA GATCCGGGAC
    TCTGACAAGG TCAATGATGT GGCCATCGGC ACCCACTTCA TCGACCTGCG CAAGATTTCC
    AACGATGGAG ACAAAGGCTT CCTGCCTACC CTCGGTCCAG CCTGGGTGAA CATGTACGGC
    TCCACGCGCA ACTACACACT GCTGGACGAG CACCAGGACT TGAATGAAGG CCTGGGGGAG
    GGTGTGTCCT TCCGGGCCCG CCTCATGTTG GGACTAGCTG TGGAGATCCT GGACACCTCC
    AACCCAGAGC TCACCAGCTC CACGGAGGTG CAGGTGGAGC AGGCCACGCC TGTCTCGGAG
    AGCTGCACAG GGAGAATGGA AGAATTTTTT CTATTTGGAG CCTTCTTGGA AGCCTCAATG
    ATTGACCGGA AAAATGGGGA CAAGCCAATT ACCTTTGAGG TGACCATAGG AAACTACGGC
    AATGAAGTCG ATGGTATGTC CCGGCCCCTG AGGCCTCGGC CCCGGAAAGA GCCTGGGGAT
    GAAGAAGAGG TAGACCTGAT TCAGAACTCC AGTGACGATG AAGGTGACGA AGCCGGGGAC
    CTGGCCTCGG TGTCCTCCAC CCCACCTATG CGGCCCCAGA TCACGGACAG GAACTATTTC
    CACCTGCCCT ACCTGGAGCG CAAGCCCTGC ATCTATATCA AGAGCTGGTG GCCTGACCAG
    AGGCGGCGCC TCTACAATGC CAACATCATG GATCACATTG CTGACAAGCT GGAAGAAGGC
    CTGAATGATG TACAGGAGAT GATCAAAACG GAGAAGTCCT ACCCGGAGCG CCGCCTGCGG
    GGTGTGCTAG AGGAACTCAG CTGTGGCTGC CACCGCTTCC TCTCCCTCTC GGACAAGGAC
    CAGGGCCGCT CGTCCCGCAC CAGGCTGGAT CGAGAGCGTC TTAAGTCCTG TATGAGGGAG
    TTGGAGAGCA TGGGACAGCA GGCCAAGAGC CTGAGGGCTC AGGTGAAGCG GCACACTGTT
    CGGGACAAGC TGAGGTCATG CCAGAACTTT CTGCAGAAGC TACGCTTCCT GGCGGATGAG
    CCCCAGCACA GCATTCCTGA TGTGTTCATT TGGATGATGA GCAACAACAA ACGTATCGCC
    TATGCCCGCG TGCCTTCCAA AGACCTGCTC TTCTCCATCG TGGAGGAGGA ACTGGGCAAG
    GACTGCGCCA AAGTCAAGAC CCTCTTCCTG AAGCTGCCAG GGAAGAGGGG CTTCGGCTCG
    GCAGGCTGGA CAGTACAGGC CAAGCTGGAG CTCTACCTGT GGCTGGGCCT CAGCAAGCAG
    CGAAAGGACT TCCTGTGTGG TCTGCCCTGT GGCTTCGAGG AGGTCAAGGC AGCCCAAGGC
    CTGGGCCTGC ATTCCTTTCC GCCCATCAGC CTAGTCTACA CCAAGAAGCA AGCCTTCCAG
    CTCCGAGCAC ACATGTATCA GGCCCGAAGC CTCTTTGCTG CTGACAGCAG TGGGCTCTCT
    GATCCCTTTG CCCGTGTCTT CTTCATCAAC CAGAGCCAAT GCACTGAGGT TCTAAACGAG
    ACACTGTGTC CCACCTGGGA CCAGATGCTG GTATTTGACA ACCTGGAGCT GTACGGTGAA
    GCTCACGAGT TACGAGATGA TCCCCCCATC ATTGTCATTG AAATCTACGA CCAGGACAGC
    ATGGGCAAAG CCGACTTCAT GGGCCGGACC TTCGCCAAGC CCCTGGTGAA GATGGCAGAT
    GAAGCATACT GCCCACCTCG CTTCCCGCCG CAGCTTGAGT ACTACCAGAT CTACCGAGGC
    AGTGCCACTG CCGGAGACCT ACTGGCTGCC TTCGAGCTGC TGCAGATTGG GCCATCAGGG
    AAGGCTGACC TGCCACCCAT CAATGGCCCA GTGGACATGG ACAGAGGGCC CATCATGCCT
    GTGCCCGTGG GAATCCGGCC AGTGCTCAGC AAGTACCGAG TGGAGGTGCT GTTCTGGGGC
    CTGAGGGACC TAAAGAGGGT GAACCTGGCC CAGGTGGACC GACCACGGGT GGACATCGAG
    TGTGCAGGAA AGGGGGTACA ATCCTCCCTG ATTCACAATT ATAAGAAGAA CCCCAACTTC
    AACACGCTGG TCAAGTGGTT TGAAGTGGAC CTCCCGGAGA ATGAGCTCCT GCACCCACCC
    TTGAACATCC GAGTGGTAGA TTGCCGGGCC TTTGGACGAT ACACCCTGGT GGGTTCCCAC
    GCAGTCAGCT CACTGAGGCG CTTCATCTAC CGACCTCCAG ACCGCTCAGC CCCCAACTGG
    AACACCACAG TCAGGCTGCT CCGGGGCTGC CACAGGCTGC GCAATGGGGG CCCCTCTTCT
    CGCCCCACAG GGGAGGTTGT AGTAAGCATG GAGCCTGAGG AGCCAGTTAA GAAGCTGGAG
    ACCATGGTGA AACTGGATGC GACTTCTGAT GCTGTGGTCA AGGTGGATGT GGCTGAAGAT
    GAGAAGGAAA GGAAGAAGAA GAAAAAGAAA GGCCCGTCAG AGGAGCCAGA GGAGGAAGAG
    CCCGATGAGA GCATGCTGGA TTGGTGGTCC AAGTACTTCG CCTCCATCGA CACAATGAAG
    GAGCAACTTC GACAACATGA GACCTCTGGA ACTGACTTGG AAGAGAAGGA AGAGATGGAA
    AGCGCTGAGG GCCTGAAGGG ACCAATGAAG AGCAAGGAGA AGTCCAGAGC TGCAAAGGAG
    GAGAAAAAGA AGAAAAACCA GAGCCCTGGC CCTGGCCAGG GATCGGAGGC TCCTGAGAAG
    AAGAAAGCCA AGATCGATGA GCTTAAGGTG TACCCCAAGG AGCTGGAATC GGAGTTTGAC
    AGCTTTGAGG ACTGGCTGCA CACCTTCAAC CTGTTGAGGG GCAAGACGGG AGATGATGAG
    GATGGCTCCA CAGAGGAGGA GCGCATAGTA GGCCGATTCA AGGGCTCCCT CTGTGTGTAC
    AAAGTGCCAC TCCCAGAAGA TGTATCTCGA GAAGCTGGCT ATGATCCCAC CTATGGAATG
    TTCCAGGGCA TCCCAAGCAA TGACCCCATC AATGTGCTGG TCCGAATCTA TGTGGTCCGG
    GCCACAGACC TGCACCCGGC CGACATCAAT GGCAAAGCTG ACCCCTATAT TGCCATCAAG
    TTAGGCAAGA CCGACATCCG AGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTG
    TTTGGGAAGT CCTTTGACAT TGAGGCCTCC TTCCCCATGG AGTCCATGTT GACAGTGGCC
    GTGTACGACT GGGATCTGGT GGGCACTGAT GACCTCATCG GAGAAACCAA GATTGACCTG
    GAAAACCGCT TCTACAGCAA GCATCGCGCC ACCTGCGGCA TCGCACAGAC CTATTCCATA
    CATGGCTACA ATATCTGGAG GGACCCCATG AAGCCCAGCC AGATCCTGAC ACGCCTCTGT
    AAAGAGGGCA AAGTGGACGG CCCCCACTTT GGTCCCCATG GGAGAGTGAG GGTTGCCAAC
    CGTGTCTTCA CGGGGCCTTC AGAAATAGAG GATGAGAATG GTCAGAGGAA GCCCACAGAT
    GAGCACGTGG CACTGTCTGC TCTGAGACAC TGGGAGGACA TCCCCCGGGT GGGCTGCCGC
    CTTGTGCCGG AACACGTGGA GACCAGGCCG CTGCTCAACC CTGACAAGCC AGGCATTGAG
    CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACA
    CCTCTGGATA TATCCCCCAG GAAACCCAAG AAGTACGAGC TGCGGGTCAT CGTGTGGAAC
    ACAGACGAGG TGGTCCTGGA AGACGATGAT TTCTTCACGG GAGAGAAGTC CAGTGACATT
    TTTGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAAC AGGACACAGA TGTCCACTAT
    CACTCCCTCA CGGGGGAGGG CAACTTCAAC TGGAGATACC TCTTCCCCTT CGACTACCTA
    GCGGCCGAAG AGAAGATCGT TATGTCCAAA AAGGAGTCTA TGTTCTCCTG GGATGAGACG
    GAGTACAAGA TCCCTGCGCG GCTCACCCTG CAGATCTGGG ACGCTGACCA CTTCTCGGCT
    GACGACTTCC TGGGGGCTAT CGAGCTGGAC CTGAACCGGT TCCCGAGGGG CGCTAAGACA
    GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTACCCCT GGTTTCCATC
    TTTAAACAGA AACGTGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAATGATGAG
    TTTGAGCTCA CAGGCAAAGT GGAGGCGGAG CTACACCTAC TCACGGCAGA GGAGGCAGAG
    AAGAACCCTG TGGGCCTGGC TCGCAATGAA CCTGATCCCC TAGAAAAACC CAATTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu35277
    Clone ID Related Accession (Same CDS sequence) XM_006504209.4 , XM_006504209.3
    Accession Version XM_006504209.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5463bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product otoferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504209.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAACTCG GCAAAACTCG GTCCCACAAA GAGGAGCCCC AAAGACAAGA TGAGCCAGCA 
    GTGCTGGAGA TGGAGGACCT GGACCACCTA GCCATTCAGC TGGGGGATGG GCTGGATCCT
    GACTCCGTGT CTCTAGCCTC GGTCACCGCT CTCACCAGCA ATGTCTCCAA CAAACGGTCT
    AAGCCAGATA TTAAGATGGA GCCCAGTGCT GGAAGGCCCA TGGATTACCA GGTCAGCATC
    ACAGTGATTG AGGCTCGGCA GCTGGTGGGC TTGAACATGG ACCCTGTGGT GTGTGTGGAG
    GTGGGTGATG ACAAGAAATA CACGTCAATG AAGGAGTCCA CAAACTGCCC TTACTACAAC
    GAGTACTTTG TCTTCGACTT CCATGTCTCT CCTGATGTCA TGTTTGACAA GATCATCAAG
    ATCTCGGTTA TCCATTCTAA GAACCTGCTT CGGAGCGGCA CCCTGGTGGG TTCCTTCAAA
    ATGGATGTGG GGACTGTGTA TTCCCAGCCT GAACACCAGT TCCATCACAA ATGGGCCATC
    CTGTCAGACC CCGATGACAT CTCTGCTGGG TTGAAGGGTT ATGTAAAGTG TGATGTCGCT
    GTGGTGGGCA AGGGAGACAA CATCAAGACA CCCCACAAGG CCAACGAGAC GGATGAGGAC
    GACATTGAAG GGAACTTGCT GCTCCCCGAG GGCGTGCCCC CCGAACGGCA GTGGGCACGG
    TTCTATGTGA AAATTTACCG AGCAGAGGGA CTGCCCCGGA TGAACACAAG CCTCATGGCC
    AACGTGAAGA AGGCGTTCAT CGGTGAGAAC AAGGACCTCG TCGACCCCTA TGTGCAAGTC
    TTCTTTGCTG GACAAAAGGG CAAAACATCA GTGCAGAAGA GCAGCTATGA GCCGCTATGG
    AATGAGCAGG TCGTCTTCAC AGACTTGTTC CCCCCACTCT GCAAACGCAT GAAGGTGCAG
    ATCCGGGACT CTGACAAGGT CAATGATGTG GCCATCGGCA CCCACTTCAT CGACCTGCGC
    AAGATTTCCA ACGATGGAGA CAAAGGCTTC CTGCCTACCC TCGGTCCAGC CTGGGTGAAC
    ATGTACGGCT CCACGCGCAA CTACACACTG CTGGACGAGC ACCAGGACTT GAATGAAGGC
    CTGGGGGAGG GTGTGTCCTT CCGGGCCCGC CTCATGTTGG GACTAGCTGT GGAGATCCTG
    GACACCTCCA ACCCAGAGCT CACCAGCTCC ACGGAGGTGC AGGTGGAGCA GGCCACGCCT
    GTCTCGGAGA GCTGCACAGG GAGAATGGAA GAATTTTTTC TATTTGGAGC CTTCTTGGAA
    GCCTCAATGA TTGACCGGAA AAATGGGGAC AAGCCAATTA CCTTTGAGGT GACCATAGGA
    AACTACGGCA ATGAAGTCGA TGGTATGTCC CGGCCCCTGA GGCCTCGGCC CCGGAAAGAG
    CCTGGGGATG AAGAAGAGGT AGACCTGATT CAGAACTCCA GTGACGATGA AGGTGACGAA
    GCCGGGGACC TGGCCTCGGT GTCCTCCACC CCACCTATGC GGCCCCAGAT CACGGACAGG
    AACTATTTCC ACCTGCCCTA CCTGGAGCGC AAGCCCTGCA TCTATATCAA GAGCTGGTGG
    CCTGACCAGA GGCGGCGCCT CTACAATGCC AACATCATGG ATCACATTGC TGACAAGCTG
    GAAGAAGGCC TGAATGATGT ACAGGAGATG ATCAAAACGG AGAAGTCCTA CCCGGAGCGC
    CGCCTGCGGG GTGTGCTAGA GGAACTCAGC TGTGGCTGCC ACCGCTTCCT CTCCCTCTCG
    GACAAGGACC AGGGCCGCTC GTCCCGCACC AGGCTGGATC GAGAGCGTCT TAAGTCCTGT
    ATGAGGGAGT TGGAGAGCAT GGGACAGCAG GCCAAGAGCC TGAGGGCTCA GGTGAAGCGG
    CACACTGTTC GGGACAAGCT GAGGTCATGC CAGAACTTTC TGCAGAAGCT ACGCTTCCTG
    GCGGATGAGC CCCAGCACAG CATTCCTGAT GTGTTCATTT GGATGATGAG CAACAACAAA
    CGTATCGCCT ATGCCCGCGT GCCTTCCAAA GACCTGCTCT TCTCCATCGT GGAGGAGGAA
    CTGGGCAAGG ACTGCGCCAA AGTCAAGACC CTCTTCCTGA AGCTGCCAGG GAAGAGGGGC
    TTCGGCTCGG CAGGCTGGAC AGTACAGGCC AAGCTGGAGC TCTACCTGTG GCTGGGCCTC
    AGCAAGCAGC GAAAGGACTT CCTGTGTGGT CTGCCCTGTG GCTTCGAGGA GGTCAAGGCA
    GCCCAAGGCC TGGGCCTGCA TTCCTTTCCG CCCATCAGCC TAGTCTACAC CAAGAAGCAA
    GCCTTCCAGC TCCGAGCACA CATGTATCAG GCCCGAAGCC TCTTTGCTGC TGACAGCAGT
    GGGCTCTCTG ATCCCTTTGC CCGTGTCTTC TTCATCAACC AGAGCCAATG CACTGAGGTT
    CTAAACGAGA CACTGTGTCC CACCTGGGAC CAGATGCTGG TATTTGACAA CCTGGAGCTG
    TACGGTGAAG CTCACGAGTT ACGAGATGAT CCCCCCATCA TTGTCATTGA AATCTACGAC
    CAGGACAGCA TGGGCAAAGC CGACTTCATG GGCCGGACCT TCGCCAAGCC CCTGGTGAAG
    ATGGCAGATG AAGCATACTG CCCACCTCGC TTCCCGCCGC AGCTTGAGTA CTACCAGATC
    TACCGAGGCA GTGCCACTGC CGGAGACCTA CTGGCTGCCT TCGAGCTGCT GCAGATTGGG
    CCATCAGGGA AGGCTGACCT GCCACCCATC AATGGCCCAG TGGACATGGA CAGAGGGCCC
    ATCATGCCTG TGCCCGTGGG AATCCGGCCA GTGCTCAGCA AGTACCGAGT GGAGGTGCTG
    TTCTGGGGCC TGAGGGACCT AAAGAGGGTG AACCTGGCCC AGGTGGACCG ACCACGGGTG
    GACATCGAGT GTGCAGGAAA GGGGGTACAA TCCTCCCTGA TTCACAATTA TAAGAAGAAC
    CCCAACTTCA ACACGCTGGT CAAGTGGTTT GAAGTGGACC TCCCGGAGAA TGAGCTCCTG
    CACCCACCCT TGAACATCCG AGTGGTAGAT TGCCGGGCCT TTGGACGATA CACCCTGGTG
    GGTTCCCACG CAGTCAGCTC ACTGAGGCGC TTCATCTACC GACCTCCAGA CCGCTCAGCC
    CCCAACTGGA ACACCACAGT CAGGCTGCTC CGGGGCTGCC ACAGGCTGCG CAATGGGGGC
    CCCTCTTCTC GCCCCACAGG GGAGGTTGTA GTAAGCATGG AGCCTGAGGA GCCAGTTAAG
    AAGCTGGAGA CCATGGTGAA ACTGGATGCG ACTTCTGATG CTGTGGTCAA GGTGGATGTG
    GCTGAAGATG AGAAGGAAAG GAAGAAGAAG AAAAAGAAAG GCCCGTCAGA GGAGCCAGAG
    GAGGAAGAGC CCGATGAGAG CATGCTGGAT TGGTGGTCCA AGTACTTCGC CTCCATCGAC
    ACAATGAAGG AGCAACTTCG ACAACATGAG ACCTCTGGAA CTGACTTGGA AGAGAAGGAA
    GAGATGGAAA GCGCTGAGGG CCTGAAGGGA CCAATGAAGA GCAAGGAGAA GTCCAGAGCT
    GCAAAGGAGG AGAAAAAGAA GAAAAACCAG AGCCCTGGCC CTGGCCAGGG ATCGGAGGCT
    CCTGAGAAGA AGAAAGCCAA GATCGATGAG CTTAAGGTGT ACCCCAAGGA GCTGGAATCG
    GAGTTTGACA GCTTTGAGGA CTGGCTGCAC ACCTTCAACC TGTTGAGGGG CAAGACGGGA
    GATGATGAGG ATGGCTCCAC AGAGGAGGAG CGCATAGTAG GCCGATTCAA GGGCTCCCTC
    TGTGTGTACA AAGTGCCACT CCCAGAAGAT GTATCTCGAG AAGCTGGCTA TGATCCCACC
    TATGGAATGT TCCAGGGCAT CCCAAGCAAT GACCCCATCA ATGTGCTGGT CCGAATCTAT
    GTGGTCCGGG CCACAGACCT GCACCCGGCC GACATCAATG GCAAAGCTGA CCCCTATATT
    GCCATCAAGT TAGGCAAGAC CGACATCCGA GACAAGGAGA ACTACATCTC CAAGCAGCTC
    AACCCTGTGT TTGGGAAGTC CTTTGACATT GAGGCCTCCT TCCCCATGGA GTCCATGTTG
    ACAGTGGCCG TGTACGACTG GGATCTGGTG GGCACTGATG ACCTCATCGG AGAAACCAAG
    ATTGACCTGG AAAACCGCTT CTACAGCAAG CATCGCGCCA CCTGCGGCAT CGCACAGACC
    TATTCCATAC ATGGCTACAA TATCTGGAGG GACCCCATGA AGCCCAGCCA GATCCTGACA
    CGCCTCTGTA AAGAGGGCAA AGTGGACGGC CCCCACTTTG GTCCCCATGG GAGAGTGAGG
    GTTGCCAACC GTGTCTTCAC GGGGCCTTCA GAAATAGAGG ATGAGAATGG TCAGAGGAAG
    CCCACAGATG AGCACGTGGC ACTGTCTGCT CTGAGACACT GGGAGGACAT CCCCCGGGTG
    GGCTGCCGCC TTGTGCCGGA ACACGTGGAG ACCAGGCCGC TGCTCAACCC TGACAAGCCA
    GGCATTGAGC AGGGCCGCCT GGAGCTGTGG GTGGACATGT TCCCCATGGA CATGCCAGCC
    CCTGGGACAC CTCTGGATAT ATCCCCCAGG AAACCCAAGA AGTACGAGCT GCGGGTCATC
    GTGTGGAACA CAGACGAGGT GGTCCTGGAA GACGATGATT TCTTCACGGG AGAGAAGTCC
    AGTGACATTT TTGTGAGGGG GTGGCTGAAG GGCCAGCAGG AGGACAAACA GGACACAGAT
    GTCCACTATC ACTCCCTCAC GGGGGAGGGC AACTTCAACT GGAGATACCT CTTCCCCTTC
    GACTACCTAG CGGCCGAAGA GAAGATCGTT ATGTCCAAAA AGGAGTCTAT GTTCTCCTGG
    GATGAGACGG AGTACAAGAT CCCTGCGCGG CTCACCCTGC AGATCTGGGA CGCTGACCAC
    TTCTCGGCTG ACGACTTCCT GGGGGCTATC GAGCTGGACC TGAACCGGTT CCCGAGGGGC
    GCTAAGACAG CCAAGCAGTG CACCATGGAG ATGGCCACCG GGGAGGTGGA CGTACCCCTG
    GTTTCCATCT TTAAACAGAA ACGTGTCAAA GGCTGGTGGC CCCTCCTGGC CCGCAATGAG
    AATGATGAGT TTGAGCTCAC AGGCAAAGTG GAGGCGGAGC TACACCTACT CACGGCAGAG
    GAGGCAGAGA AGAACCCTGT GGGCCTGGCT CGCAATGAAC CTGATCCCCT AGAAAAACCC
    AATCGGCCGG ACACAAGCTT CATCTGGTTC TTGAACCCTC TCAAGTCTGC CCGCTACTTC
    CTGTGGCATA CCTACCGCTG GCTACTCCTC AAATTCCTGC TGCTCTTCCT CCTGCTGCTG
    CTCTTCGCCC TGTTTCTCTA CTCTCTGCCT GGCTACCTGG CCAAGAAGAT CCTTGGGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006504272.1
    CDS79..5541
    Translation

    Target ORF information:

    RefSeq Version XM_006504209.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus otoferlin (Otof), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006504209.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAACTCG GCAAAACTCG GTCCCACAAA GAGGAGCCCC AAAGACAAGA TGAGCCAGCA 
    GTGCTGGAGA TGGAGGACCT GGACCACCTA GCCATTCAGC TGGGGGATGG GCTGGATCCT
    GACTCCGTGT CTCTAGCCTC GGTCACCGCT CTCACCAGCA ATGTCTCCAA CAAACGGTCT
    AAGCCAGATA TTAAGATGGA GCCCAGTGCT GGAAGGCCCA TGGATTACCA GGTCAGCATC
    ACAGTGATTG AGGCTCGGCA GCTGGTGGGC TTGAACATGG ACCCTGTGGT GTGTGTGGAG
    GTGGGTGATG ACAAGAAATA CACGTCAATG AAGGAGTCCA CAAACTGCCC TTACTACAAC
    GAGTACTTTG TCTTCGACTT CCATGTCTCT CCTGATGTCA TGTTTGACAA GATCATCAAG
    ATCTCGGTTA TCCATTCTAA GAACCTGCTT CGGAGCGGCA CCCTGGTGGG TTCCTTCAAA
    ATGGATGTGG GGACTGTGTA TTCCCAGCCT GAACACCAGT TCCATCACAA ATGGGCCATC
    CTGTCAGACC CCGATGACAT CTCTGCTGGG TTGAAGGGTT ATGTAAAGTG TGATGTCGCT
    GTGGTGGGCA AGGGAGACAA CATCAAGACA CCCCACAAGG CCAACGAGAC GGATGAGGAC
    GACATTGAAG GGAACTTGCT GCTCCCCGAG GGCGTGCCCC CCGAACGGCA GTGGGCACGG
    TTCTATGTGA AAATTTACCG AGCAGAGGGA CTGCCCCGGA TGAACACAAG CCTCATGGCC
    AACGTGAAGA AGGCGTTCAT CGGTGAGAAC AAGGACCTCG TCGACCCCTA TGTGCAAGTC
    TTCTTTGCTG GACAAAAGGG CAAAACATCA GTGCAGAAGA GCAGCTATGA GCCGCTATGG
    AATGAGCAGG TCGTCTTCAC AGACTTGTTC CCCCCACTCT GCAAACGCAT GAAGGTGCAG
    ATCCGGGACT CTGACAAGGT CAATGATGTG GCCATCGGCA CCCACTTCAT CGACCTGCGC
    AAGATTTCCA ACGATGGAGA CAAAGGCTTC CTGCCTACCC TCGGTCCAGC CTGGGTGAAC
    ATGTACGGCT CCACGCGCAA CTACACACTG CTGGACGAGC ACCAGGACTT GAATGAAGGC
    CTGGGGGAGG GTGTGTCCTT CCGGGCCCGC CTCATGTTGG GACTAGCTGT GGAGATCCTG
    GACACCTCCA ACCCAGAGCT CACCAGCTCC ACGGAGGTGC AGGTGGAGCA GGCCACGCCT
    GTCTCGGAGA GCTGCACAGG GAGAATGGAA GAATTTTTTC TATTTGGAGC CTTCTTGGAA
    GCCTCAATGA TTGACCGGAA AAATGGGGAC AAGCCAATTA CCTTTGAGGT GACCATAGGA
    AACTACGGCA ATGAAGTCGA TGGTATGTCC CGGCCCCTGA GGCCTCGGCC CCGGAAAGAG
    CCTGGGGATG AAGAAGAGGT AGACCTGATT CAGAACTCCA GTGACGATGA AGGTGACGAA
    GCCGGGGACC TGGCCTCGGT GTCCTCCACC CCACCTATGC GGCCCCAGAT CACGGACAGG
    AACTATTTCC ACCTGCCCTA CCTGGAGCGC AAGCCCTGCA TCTATATCAA GAGCTGGTGG
    CCTGACCAGA GGCGGCGCCT CTACAATGCC AACATCATGG ATCACATTGC TGACAAGCTG
    GAAGAAGGCC TGAATGATGT ACAGGAGATG ATCAAAACGG AGAAGTCCTA CCCGGAGCGC
    CGCCTGCGGG GTGTGCTAGA GGAACTCAGC TGTGGCTGCC ACCGCTTCCT CTCCCTCTCG
    GACAAGGACC AGGGCCGCTC GTCCCGCACC AGGCTGGATC GAGAGCGTCT TAAGTCCTGT
    ATGAGGGAGT TGGAGAGCAT GGGACAGCAG GCCAAGAGCC TGAGGGCTCA GGTGAAGCGG
    CACACTGTTC GGGACAAGCT GAGGTCATGC CAGAACTTTC TGCAGAAGCT ACGCTTCCTG
    GCGGATGAGC CCCAGCACAG CATTCCTGAT GTGTTCATTT GGATGATGAG CAACAACAAA
    CGTATCGCCT ATGCCCGCGT GCCTTCCAAA GACCTGCTCT TCTCCATCGT GGAGGAGGAA
    CTGGGCAAGG ACTGCGCCAA AGTCAAGACC CTCTTCCTGA AGCTGCCAGG GAAGAGGGGC
    TTCGGCTCGG CAGGCTGGAC AGTACAGGCC AAGCTGGAGC TCTACCTGTG GCTGGGCCTC
    AGCAAGCAGC GAAAGGACTT CCTGTGTGGT CTGCCCTGTG GCTTCGAGGA GGTCAAGGCA
    GCCCAAGGCC TGGGCCTGCA TTCCTTTCCG CCCATCAGCC TAGTCTACAC CAAGAAGCAA
    GCCTTCCAGC TCCGAGCACA CATGTATCAG GCCCGAAGCC TCTTTGCTGC TGACAGCAGT
    GGGCTCTCTG ATCCCTTTGC CCGTGTCTTC TTCATCAACC AGAGCCAATG CACTGAGGTT
    CTAAACGAGA CACTGTGTCC CACCTGGGAC CAGATGCTGG TATTTGACAA CCTGGAGCTG
    TACGGTGAAG CTCACGAGTT ACGAGATGAT CCCCCCATCA TTGTCATTGA AATCTACGAC
    CAGGACAGCA TGGGCAAAGC CGACTTCATG GGCCGGACCT TCGCCAAGCC CCTGGTGAAG
    ATGGCAGATG AAGCATACTG CCCACCTCGC TTCCCGCCGC AGCTTGAGTA CTACCAGATC
    TACCGAGGCA GTGCCACTGC CGGAGACCTA CTGGCTGCCT TCGAGCTGCT GCAGATTGGG
    CCATCAGGGA AGGCTGACCT GCCACCCATC AATGGCCCAG TGGACATGGA CAGAGGGCCC
    ATCATGCCTG TGCCCGTGGG AATCCGGCCA GTGCTCAGCA AGTACCGAGT GGAGGTGCTG
    TTCTGGGGCC TGAGGGACCT AAAGAGGGTG AACCTGGCCC AGGTGGACCG ACCACGGGTG
    GACATCGAGT GTGCAGGAAA GGGGGTACAA TCCTCCCTGA TTCACAATTA TAAGAAGAAC
    CCCAACTTCA ACACGCTGGT CAAGTGGTTT GAAGTGGACC TCCCGGAGAA TGAGCTCCTG
    CACCCACCCT TGAACATCCG AGTGGTAGAT TGCCGGGCCT TTGGACGATA CACCCTGGTG
    GGTTCCCACG CAGTCAGCTC ACTGAGGCGC TTCATCTACC GACCTCCAGA CCGCTCAGCC
    CCCAACTGGA ACACCACAGT CAGGCTGCTC CGGGGCTGCC ACAGGCTGCG CAATGGGGGC
    CCCTCTTCTC GCCCCACAGG GGAGGTTGTA GTAAGCATGG AGCCTGAGGA GCCAGTTAAG
    AAGCTGGAGA CCATGGTGAA ACTGGATGCG ACTTCTGATG CTGTGGTCAA GGTGGATGTG
    GCTGAAGATG AGAAGGAAAG GAAGAAGAAG AAAAAGAAAG GCCCGTCAGA GGAGCCAGAG
    GAGGAAGAGC CCGATGAGAG CATGCTGGAT TGGTGGTCCA AGTACTTCGC CTCCATCGAC
    ACAATGAAGG AGCAACTTCG ACAACATGAG ACCTCTGGAA CTGACTTGGA AGAGAAGGAA
    GAGATGGAAA GCGCTGAGGG CCTGAAGGGA CCAATGAAGA GCAAGGAGAA GTCCAGAGCT
    GCAAAGGAGG AGAAAAAGAA GAAAAACCAG AGCCCTGGCC CTGGCCAGGG ATCGGAGGCT
    CCTGAGAAGA AGAAAGCCAA GATCGATGAG CTTAAGGTGT ACCCCAAGGA GCTGGAATCG
    GAGTTTGACA GCTTTGAGGA CTGGCTGCAC ACCTTCAACC TGTTGAGGGG CAAGACGGGA
    GATGATGAGG ATGGCTCCAC AGAGGAGGAG CGCATAGTAG GCCGATTCAA GGGCTCCCTC
    TGTGTGTACA AAGTGCCACT CCCAGAAGAT GTATCTCGAG AAGCTGGCTA TGATCCCACC
    TATGGAATGT TCCAGGGCAT CCCAAGCAAT GACCCCATCA ATGTGCTGGT CCGAATCTAT
    GTGGTCCGGG CCACAGACCT GCACCCGGCC GACATCAATG GCAAAGCTGA CCCCTATATT
    GCCATCAAGT TAGGCAAGAC CGACATCCGA GACAAGGAGA ACTACATCTC CAAGCAGCTC
    AACCCTGTGT TTGGGAAGTC CTTTGACATT GAGGCCTCCT TCCCCATGGA GTCCATGTTG
    ACAGTGGCCG TGTACGACTG GGATCTGGTG GGCACTGATG ACCTCATCGG AGAAACCAAG
    ATTGACCTGG AAAACCGCTT CTACAGCAAG CATCGCGCCA CCTGCGGCAT CGCACAGACC
    TATTCCATAC ATGGCTACAA TATCTGGAGG GACCCCATGA AGCCCAGCCA GATCCTGACA
    CGCCTCTGTA AAGAGGGCAA AGTGGACGGC CCCCACTTTG GTCCCCATGG GAGAGTGAGG
    GTTGCCAACC GTGTCTTCAC GGGGCCTTCA GAAATAGAGG ATGAGAATGG TCAGAGGAAG
    CCCACAGATG AGCACGTGGC ACTGTCTGCT CTGAGACACT GGGAGGACAT CCCCCGGGTG
    GGCTGCCGCC TTGTGCCGGA ACACGTGGAG ACCAGGCCGC TGCTCAACCC TGACAAGCCA
    GGCATTGAGC AGGGCCGCCT GGAGCTGTGG GTGGACATGT TCCCCATGGA CATGCCAGCC
    CCTGGGACAC CTCTGGATAT ATCCCCCAGG AAACCCAAGA AGTACGAGCT GCGGGTCATC
    GTGTGGAACA CAGACGAGGT GGTCCTGGAA GACGATGATT TCTTCACGGG AGAGAAGTCC
    AGTGACATTT TTGTGAGGGG GTGGCTGAAG GGCCAGCAGG AGGACAAACA GGACACAGAT
    GTCCACTATC ACTCCCTCAC GGGGGAGGGC AACTTCAACT GGAGATACCT CTTCCCCTTC
    GACTACCTAG CGGCCGAAGA GAAGATCGTT ATGTCCAAAA AGGAGTCTAT GTTCTCCTGG
    GATGAGACGG AGTACAAGAT CCCTGCGCGG CTCACCCTGC AGATCTGGGA CGCTGACCAC
    TTCTCGGCTG ACGACTTCCT GGGGGCTATC GAGCTGGACC TGAACCGGTT CCCGAGGGGC
    GCTAAGACAG CCAAGCAGTG CACCATGGAG ATGGCCACCG GGGAGGTGGA CGTACCCCTG
    GTTTCCATCT TTAAACAGAA ACGTGTCAAA GGCTGGTGGC CCCTCCTGGC CCGCAATGAG
    AATGATGAGT TTGAGCTCAC AGGCAAAGTG GAGGCGGAGC TACACCTACT CACGGCAGAG
    GAGGCAGAGA AGAACCCTGT GGGCCTGGCT CGCAATGAAC CTGATCCCCT AGAAAAACCC
    AATCGGCCGG ACACAAGCTT CATCTGGTTC TTGAACCCTC TCAAGTCTGC CCGCTACTTC
    CTGTGGCATA CCTACCGCTG GCTACTCCTC AAATTCCTGC TGCTCTTCCT CCTGCTGCTG
    CTCTTCGCCC TGTTTCTCTA CTCTCTGCCT GGCTACCTGG CCAAGAAGAT CCTTGGGGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Otoferlin acts as a Ca
    eLife6(2017 Nov)
    Michalski N,Goutman JD,Auclair SM,Boutet de Monvel J,Tertrais M,Emptoz A,Parrin A,Nouaille S,Guillon M,Sachse M,Ciric D,Bahloul A,Hardelin JP,Sutton RB,Avan P,Krishnakumar SS,Rothman JE,Dulon D,Safieddine S,Petit C