otof cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following otof gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the otof cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa58168 XM_031901880.1
Latest version!
Xenopus tropicalis otoferlin (otof), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa58169 XM_031901881.1
Latest version!
Xenopus tropicalis otoferlin (otof), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa58170 XM_031901882.1
Latest version!
Xenopus tropicalis otoferlin (otof), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa58171 XM_031901883.1
Latest version!
Xenopus tropicalis otoferlin (otof), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa58172 XM_031901884.1
Latest version!
Xenopus tropicalis otoferlin (otof), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa58173 XM_031901885.1
Latest version!
Xenopus tropicalis otoferlin (otof), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa30655 XM_012971322.1
Latest version!
Xenopus tropicalis otoferlin (otof), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa58168
    Clone ID Related Accession (Same CDS sequence) XM_031901880.1
    Accession Version XM_031901880.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product otoferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
    GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG
    AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG
    TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG
    ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT
    TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG
    CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG
    AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT
    CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC
    CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT
    AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT
    ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT
    TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA
    ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC
    TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT
    ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG
    ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC
    TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG
    GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG
    ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT
    TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC
    ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC
    CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC
    CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG
    GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA
    TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC
    AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG
    AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC
    ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG
    TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA
    ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA
    ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG
    CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA
    GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA
    CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC
    AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA
    GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC
    CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG
    GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA
    GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT
    TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGCTAAACT CATGAATGGC
    TACTTGGCAA TGACCAATGG AAGGCCCCGC TCTCGCACCA CAGGTGAAAT AGTTATCAAC
    ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC
    GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG
    AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC
    AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC
    GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG AGATCAAAAC TGATGACTCT
    CCCATGAAAG GCAACAAGGG CCCCACGAAG AACAAGGAGA AGGTCAAAGC CCCAAAAGAG
    GAGAAGAAGA ACAAGAAGCA GCAACAGCAG CAGCAACAAG CCGATCAGGC CGAGAAGAAG
    AACAAGCAAA AGATCGATGA GTTAAAGGTG TACAACAAGG AGTTGGAGAC CGAGTATGAC
    AACTTTGAGG ACTGGCTCCA TACATTTAAC TTGTTACGAG GGAAGATTGG AGATGATGAC
    GATACTGTAA GCGAAGAAGA GCGAATTGTT GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC
    AAAGTTCCTT TGCCAGAAGA CGTCACAAAG GAAGCCGGGT ATGACCCAAA CTTCGGCATG
    TTCCAAGGAA TCCCTAACAA CGACCCAATG AATGTGCTGG TCAGAGTTTA TGTCGTGAGG
    GCCACCGATC TTCATCCAGC TGATATCAAT GGTAAAGCGG ATCCATATGT TGTCATAAAA
    CTAGGAAAAA CTGATATAAA AGATAAGGAA AACTACATCT CCAAGCAGCT AAACCCTGTC
    TTCGGAAAGT CCTTTGATAT TGAGGCCACT TTTCCGATGG AGTCCATGCT TACAGTTGCG
    GTTTATGACT GGGATCTGGT GGGGACGGAT GATCTGATCG GTGAAACCAA AATCGACCTG
    GAAAACCGTT ACTACAGCAA GCACAGGGCG ACTTGTGGTA TCTCAAAGAC ATATGCCATT
    CACGGGTACA ACTTGTGGAG GGACCCAGTG AAGCCTACAC AAATCTTATC CAAACTTTGC
    AAAGAAGGGA AGATCGATGG ACCTCATTAT GGCCCTGGTG GAAGAGTGAA AGTCACGAAT
    CGGGTATTCA CTGGTCCCAC AGAAATTGAT GATGAAAATG GTCAGAAGAA ACAAACGGAT
    GAGCACCTTG CACTCTCGGT CCTTCAGCAC TGGGAGGACA TTCCTCGGGT GGGATGTAAG
    CTGGTCCCAG AGCATGTAGA AACGAGGCCT TTGTTAAACC CAGACAAACC TGGAATTGAG
    CAGGGCCGCA TAGAAATGTG GGTAGACATG TTCCCCATGG ACATGCCTGC GCCAGGTCCT
    GCCATTGATA TCTCTCCAAG GAAGCCAAAA AGTTTTGAGC TACGTGTTAT TATTTGGAAC
    ACTGATGAAG TGATTCTAGA AGATGATGCT TTCATGACAG GAGAGAAGAT GTCTGACATC
    TATGTCAAGG GGTGGCTGAA GGGACAGCAG GAAGACAAAC AAGACACGGA TGTCCACTAT
    CATTCCCTTA CTGGGGAGGG GAACTTCAAC TGGAGGTTCT TGTTCCCTTT TGATTACCTT
    ATGGCAGAGG AGAAGATTGT CATCTCCAAG AAAGAGTCCA TGTTCTCTTG GGATGAGACC
    GAGTACAAGA TCCCGGCTCG TCTCACTCTC CAGGTCTGGG ATGCCGACCA CTTCTCAGCA
    GATGACTTTC TCGGAGCCAT AGAATTAGAC TTGAATCGCT TTCCCCGAGG GGCAAAAACA
    GCGAAACAGT GTTCGATAGA AATGGCGGAA CCCGACGTCG ATCTGCCAAT GGTCTCAATC
    TTCAAACAGA AGAGGGTGAA GGGATGGTGG CCTTTCGTTG CCAGGAATGA AAACGATGAG
    ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG CTTCACCTTC TCTCAGCAGA GGAGGCAGAG
    AAGACCCCGG CAGGGCTTGG GCGCAATGAA CCAGATCCTC TTGAAAAACC AAATCGGCCG
    AATCCCAGCC TGGCGTGGAT GCTGAGCCCC CTGCGGGTAG TGTGCATGTT GCTATGGCGA
    CAGTACCGGT GGCACGTACT GGGCATCATC GGTTCCTGTG CGGTTCTGAT GTTCGTGGCG
    TTGGTGCTCT ACACCCTCCC CGGCGCTATC AGCGAGAGGC TCATTAACCC CCGCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757740.1
    CDS97..4836
    Translation

    Target ORF information:

    RefSeq Version XM_031901880.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis otoferlin (otof), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901880.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
    GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG
    AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG
    TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG
    ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT
    TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG
    CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG
    AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT
    CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC
    CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT
    AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT
    ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT
    TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA
    ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC
    TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT
    ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG
    ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC
    TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG
    GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG
    ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT
    TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC
    ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC
    CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC
    CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG
    GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA
    TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC
    AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG
    AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC
    ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG
    TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA
    ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA
    ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG
    CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA
    GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA
    CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC
    AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA
    GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC
    CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG
    GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA
    GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT
    TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGCTAAACT CATGAATGGC
    TACTTGGCAA TGACCAATGG AAGGCCCCGC TCTCGCACCA CAGGTGAAAT AGTTATCAAC
    ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC
    GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG
    AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC
    AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC
    GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG AGATCAAAAC TGATGACTCT
    CCCATGAAAG GCAACAAGGG CCCCACGAAG AACAAGGAGA AGGTCAAAGC CCCAAAAGAG
    GAGAAGAAGA ACAAGAAGCA GCAACAGCAG CAGCAACAAG CCGATCAGGC CGAGAAGAAG
    AACAAGCAAA AGATCGATGA GTTAAAGGTG TACAACAAGG AGTTGGAGAC CGAGTATGAC
    AACTTTGAGG ACTGGCTCCA TACATTTAAC TTGTTACGAG GGAAGATTGG AGATGATGAC
    GATACTGTAA GCGAAGAAGA GCGAATTGTT GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC
    AAAGTTCCTT TGCCAGAAGA CGTCACAAAG GAAGCCGGGT ATGACCCAAA CTTCGGCATG
    TTCCAAGGAA TCCCTAACAA CGACCCAATG AATGTGCTGG TCAGAGTTTA TGTCGTGAGG
    GCCACCGATC TTCATCCAGC TGATATCAAT GGTAAAGCGG ATCCATATGT TGTCATAAAA
    CTAGGAAAAA CTGATATAAA AGATAAGGAA AACTACATCT CCAAGCAGCT AAACCCTGTC
    TTCGGAAAGT CCTTTGATAT TGAGGCCACT TTTCCGATGG AGTCCATGCT TACAGTTGCG
    GTTTATGACT GGGATCTGGT GGGGACGGAT GATCTGATCG GTGAAACCAA AATCGACCTG
    GAAAACCGTT ACTACAGCAA GCACAGGGCG ACTTGTGGTA TCTCAAAGAC ATATGCCATT
    CACGGGTACA ACTTGTGGAG GGACCCAGTG AAGCCTACAC AAATCTTATC CAAACTTTGC
    AAAGAAGGGA AGATCGATGG ACCTCATTAT GGCCCTGGTG GAAGAGTGAA AGTCACGAAT
    CGGGTATTCA CTGGTCCCAC AGAAATTGAT GATGAAAATG GTCAGAAGAA ACAAACGGAT
    GAGCACCTTG CACTCTCGGT CCTTCAGCAC TGGGAGGACA TTCCTCGGGT GGGATGTAAG
    CTGGTCCCAG AGCATGTAGA AACGAGGCCT TTGTTAAACC CAGACAAACC TGGAATTGAG
    CAGGGCCGCA TAGAAATGTG GGTAGACATG TTCCCCATGG ACATGCCTGC GCCAGGTCCT
    GCCATTGATA TCTCTCCAAG GAAGCCAAAA AGTTTTGAGC TACGTGTTAT TATTTGGAAC
    ACTGATGAAG TGATTCTAGA AGATGATGCT TTCATGACAG GAGAGAAGAT GTCTGACATC
    TATGTCAAGG GGTGGCTGAA GGGACAGCAG GAAGACAAAC AAGACACGGA TGTCCACTAT
    CATTCCCTTA CTGGGGAGGG GAACTTCAAC TGGAGGTTCT TGTTCCCTTT TGATTACCTT
    ATGGCAGAGG AGAAGATTGT CATCTCCAAG AAAGAGTCCA TGTTCTCTTG GGATGAGACC
    GAGTACAAGA TCCCGGCTCG TCTCACTCTC CAGGTCTGGG ATGCCGACCA CTTCTCAGCA
    GATGACTTTC TCGGAGCCAT AGAATTAGAC TTGAATCGCT TTCCCCGAGG GGCAAAAACA
    GCGAAACAGT GTTCGATAGA AATGGCGGAA CCCGACGTCG ATCTGCCAAT GGTCTCAATC
    TTCAAACAGA AGAGGGTGAA GGGATGGTGG CCTTTCGTTG CCAGGAATGA AAACGATGAG
    ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG CTTCACCTTC TCTCAGCAGA GGAGGCAGAG
    AAGACCCCGG CAGGGCTTGG GCGCAATGAA CCAGATCCTC TTGAAAAACC AAATCGGCCG
    AATCCCAGCC TGGCGTGGAT GCTGAGCCCC CTGCGGGTAG TGTGCATGTT GCTATGGCGA
    CAGTACCGGT GGCACGTACT GGGCATCATC GGTTCCTGTG CGGTTCTGAT GTTCGTGGCG
    TTGGTGCTCT ACACCCTCCC CGGCGCTATC AGCGAGAGGC TCATTAACCC CCGCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58169
    Clone ID Related Accession (Same CDS sequence) XM_031901881.1
    Accession Version XM_031901881.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4734bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product otoferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
    GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG
    AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG
    TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG
    ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT
    TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG
    CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG
    AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT
    CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC
    CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT
    AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT
    ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT
    TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA
    ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC
    TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT
    ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG
    ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC
    TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG
    GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG
    ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT
    TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC
    ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC
    CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC
    CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG
    GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA
    TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC
    AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG
    AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC
    ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG
    TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA
    ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA
    ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG
    CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA
    GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA
    CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC
    AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA
    GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC
    CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG
    GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA
    GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT
    TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGCTAAACT CATGAATGGC
    TACTTGGCAA TGACCAATGG AAGGCCCCGC TCTCGCACCA CAGGTGAAAT AGTTATCAAC
    ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC
    GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG
    AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC
    AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC
    GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG AGATCAAAAC TGATGACTCT
    CCCATGAAAG GCAACAAGGG CCCCACGAAG AACAAGGAGA AGGTCAAAGC CCCAAAAGAG
    GAGAAGAAGA ACAAGAAGCA GCAACAGCAG CAGCAACAAG CCGATCAGGC CGAGAAGAAG
    AACAAGCAAA AGATCGATGA GTTAAAGGTG TACAACAAGG AGTTGGAGAC CGAGTATGAC
    AACTTTGAGG ACTGGCTCCA TACATTTAAC TTGTTACGAG GGAAGATTGG AGATGATGAC
    GATACTGTAA GCGAAGAAGA GCGAATTGTT GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC
    AAAGTTCCTT TGCCAGAAGA CGTCACAAAG GAAGCCGGGT ATGACCCAAA CTTCGGCATG
    TTCCAAGGAA TCCCTAACAA CGACCCAATG AATGTGCTGG TCAGAGTTTA TGTCGTGAGG
    GCCACCGATC TTCATCCAGC TGATATCAAT GGTAAAGCGG ATCCATATGT TGTCATAAAA
    CTAGGAAAAA CTGATATAAA AGATAAGGAA AACTACATCT CCAAGCAGCT AAACCCTGTC
    TTCGGAAAGT CCTTTGATAT TGAGGCCACT TTTCCGATGG AGTCCATGCT TACAGTTGCG
    GTTTATGACT GGGATCTGGT GGGGACGGAT GATCTGATCG GTGAAACCAA AATCGACCTG
    GAAAACCGTT ACTACAGCAA GCACAGGGCG ACTTGTGGTA TCTCAAAGAC ATATGCCATT
    CACGGGTACA ACTTGTGGAG GGACCCAGTG AAGCCTACAC AAATCTTATC CAAACTTTGC
    AAAGAAGGGA AGATCGATGG ACCTCATTAT GGCCCTGGTG GAAGAGTGAA AGTCACGAAT
    CGGGTATTCA CTGGTCCCAC AGAAATTGAT GATGAAAATG GTCAGAAGAA ACAAACGGAT
    GAGCACCTTG CACTCTCGGT CCTTCAGCAC TGGGAGGACA TTCCTCGGGT GGGATGTAAG
    CTGGTCCCAG AGCATGTAGA AACGAGGCCT TTGTTAAACC CAGACAAACC TGGAATTGAG
    CAGGGCCGCA TAGAAATGTG GGTAGACATG TTCCCCATGG ACATGCCTGC GCCAGGTCCT
    GCCATTGATA TCTCTCCAAG GAAGCCAAAA AGATACGAGC TTAGAGTGAT AATATGGAAT
    ACAGATGAGG TAATCCTGGA AGATGATGAT TACTTCACAG GAGAAAAATC TAGTGACATA
    TTTGTCAGGG GGTGGCTGAA GGGACAGCAG GAAGACAAAC AAGACACGGA TGTCCACTAT
    CATTCCCTTA CTGGGGAGGG GAACTTCAAC TGGAGGTTCT TGTTCCCTTT TGATTACCTT
    ATGGCAGAGG AGAAGATTGT CATCTCCAAG AAAGAGTCCA TGTTCTCTTG GGATGAGACC
    GAGTACAAGA TCCCGGCTCG TCTCACTCTC CAGGTCTGGG ATGCCGACCA CTTCTCAGCA
    GATGACTTTC TCGGAGCCAT AGAATTAGAC TTGAATCGCT TTCCCCGAGG GGCAAAAACA
    GCGAAACAGT GTTCGATAGA AATGGCGGAA CCCGACGTCG ATCTGCCAAT GGTCTCAATC
    TTCAAACAGA AGAGGGTGAA GGGATGGTGG CCTTTCGTTG CCAGGAATGA AAACGATGAG
    ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG CTTCACCTTC TCTCAGCAGA GGAGGCAGAG
    AAGACCCCGG CAGGGCTTGG GCGCAATGAA CCAGATCCTC TTGAAAAACC AAATCGACCC
    GACACCTCGT TCATCTGGTT CCTCAACCCA CTTAAGTCCA TCAAGTATCT CATCTGTAAT
    CGCTACAAGT GGCTCATCAT TAAGATTGTG CTGGCGTTGC TGCTTTTGGC CATGGTGGCC
    CTCTTCCTCT ACAGCATGCC CGGTTACATG GTGAAGAAGC TTCTGGGTGC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757741.1
    CDS97..4830
    Translation

    Target ORF information:

    RefSeq Version XM_031901881.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis otoferlin (otof), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901881.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
    GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG
    AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG
    TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG
    ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT
    TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG
    CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG
    AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT
    CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC
    CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT
    AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT
    ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT
    TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA
    ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC
    TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT
    ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG
    ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC
    TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG
    GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG
    ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT
    TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC
    ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC
    CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC
    CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG
    GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA
    TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC
    AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG
    AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC
    ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG
    TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA
    ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA
    ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG
    CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA
    GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA
    CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC
    AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA
    GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC
    CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG
    GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA
    GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT
    TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGCTAAACT CATGAATGGC
    TACTTGGCAA TGACCAATGG AAGGCCCCGC TCTCGCACCA CAGGTGAAAT AGTTATCAAC
    ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC
    GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG
    AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC
    AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC
    GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG AGATCAAAAC TGATGACTCT
    CCCATGAAAG GCAACAAGGG CCCCACGAAG AACAAGGAGA AGGTCAAAGC CCCAAAAGAG
    GAGAAGAAGA ACAAGAAGCA GCAACAGCAG CAGCAACAAG CCGATCAGGC CGAGAAGAAG
    AACAAGCAAA AGATCGATGA GTTAAAGGTG TACAACAAGG AGTTGGAGAC CGAGTATGAC
    AACTTTGAGG ACTGGCTCCA TACATTTAAC TTGTTACGAG GGAAGATTGG AGATGATGAC
    GATACTGTAA GCGAAGAAGA GCGAATTGTT GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC
    AAAGTTCCTT TGCCAGAAGA CGTCACAAAG GAAGCCGGGT ATGACCCAAA CTTCGGCATG
    TTCCAAGGAA TCCCTAACAA CGACCCAATG AATGTGCTGG TCAGAGTTTA TGTCGTGAGG
    GCCACCGATC TTCATCCAGC TGATATCAAT GGTAAAGCGG ATCCATATGT TGTCATAAAA
    CTAGGAAAAA CTGATATAAA AGATAAGGAA AACTACATCT CCAAGCAGCT AAACCCTGTC
    TTCGGAAAGT CCTTTGATAT TGAGGCCACT TTTCCGATGG AGTCCATGCT TACAGTTGCG
    GTTTATGACT GGGATCTGGT GGGGACGGAT GATCTGATCG GTGAAACCAA AATCGACCTG
    GAAAACCGTT ACTACAGCAA GCACAGGGCG ACTTGTGGTA TCTCAAAGAC ATATGCCATT
    CACGGGTACA ACTTGTGGAG GGACCCAGTG AAGCCTACAC AAATCTTATC CAAACTTTGC
    AAAGAAGGGA AGATCGATGG ACCTCATTAT GGCCCTGGTG GAAGAGTGAA AGTCACGAAT
    CGGGTATTCA CTGGTCCCAC AGAAATTGAT GATGAAAATG GTCAGAAGAA ACAAACGGAT
    GAGCACCTTG CACTCTCGGT CCTTCAGCAC TGGGAGGACA TTCCTCGGGT GGGATGTAAG
    CTGGTCCCAG AGCATGTAGA AACGAGGCCT TTGTTAAACC CAGACAAACC TGGAATTGAG
    CAGGGCCGCA TAGAAATGTG GGTAGACATG TTCCCCATGG ACATGCCTGC GCCAGGTCCT
    GCCATTGATA TCTCTCCAAG GAAGCCAAAA AGATACGAGC TTAGAGTGAT AATATGGAAT
    ACAGATGAGG TAATCCTGGA AGATGATGAT TACTTCACAG GAGAAAAATC TAGTGACATA
    TTTGTCAGGG GGTGGCTGAA GGGACAGCAG GAAGACAAAC AAGACACGGA TGTCCACTAT
    CATTCCCTTA CTGGGGAGGG GAACTTCAAC TGGAGGTTCT TGTTCCCTTT TGATTACCTT
    ATGGCAGAGG AGAAGATTGT CATCTCCAAG AAAGAGTCCA TGTTCTCTTG GGATGAGACC
    GAGTACAAGA TCCCGGCTCG TCTCACTCTC CAGGTCTGGG ATGCCGACCA CTTCTCAGCA
    GATGACTTTC TCGGAGCCAT AGAATTAGAC TTGAATCGCT TTCCCCGAGG GGCAAAAACA
    GCGAAACAGT GTTCGATAGA AATGGCGGAA CCCGACGTCG ATCTGCCAAT GGTCTCAATC
    TTCAAACAGA AGAGGGTGAA GGGATGGTGG CCTTTCGTTG CCAGGAATGA AAACGATGAG
    ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG CTTCACCTTC TCTCAGCAGA GGAGGCAGAG
    AAGACCCCGG CAGGGCTTGG GCGCAATGAA CCAGATCCTC TTGAAAAACC AAATCGACCC
    GACACCTCGT TCATCTGGTT CCTCAACCCA CTTAAGTCCA TCAAGTATCT CATCTGTAAT
    CGCTACAAGT GGCTCATCAT TAAGATTGTG CTGGCGTTGC TGCTTTTGGC CATGGTGGCC
    CTCTTCCTCT ACAGCATGCC CGGTTACATG GTGAAGAAGC TTCTGGGTGC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58170
    Clone ID Related Accession (Same CDS sequence) XM_031901882.1
    Accession Version XM_031901882.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4734bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product otoferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
    GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG
    AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG
    TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG
    ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT
    TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG
    CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG
    AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT
    CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC
    CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT
    AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT
    ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT
    TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA
    ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC
    TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT
    ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG
    ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC
    TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG
    GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG
    ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT
    TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC
    ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC
    CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC
    CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG
    GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA
    TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC
    AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG
    AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC
    ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG
    TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA
    ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA
    ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG
    CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA
    GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA
    CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC
    AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA
    GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC
    CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG
    GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA
    GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT
    TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGCTAAACT CATGAATGGC
    TACTTGGCAA TGACCAATGG AAGGCCCCGC TCTCGCACCA CAGGTGAAAT AGTTATCAAC
    ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC
    GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG
    AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC
    AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC
    GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG AGATCAAAAC TGATGACTCT
    CCCATGAAAG GCAACAAGGG CCCCACGAAG AACAAGGAGA AGGTCAAAGC CCCAAAAGAG
    GAGAAGAAGA ACAAGAAGCA GCAACAGCAG CAGCAACAAG CCGATCAGGC CGAGAAGAAG
    AACAAGCAAA AGATCGATGA GTTAAAGGTG TACAACAAGG AGTTGGAGAC CGAGTATGAC
    AACTTTGAGG ACTGGCTCCA TACATTTAAC TTGTTACGAG GGAAGATTGG AGATGATGAC
    GATACTGTAA GCGAAGAAGA GCGAATTGTT GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC
    AAAGTTCCTT TGCCAGAAGA CGTCACAAAG GAAGCCGGGT ATGACCCAAA CTTCGGCATG
    TTCCAAGGAA TCCCTAACAA CGACCCAATG AATGTGCTGG TCAGAGTTTA TGTCGTGAGG
    GCCACCGATC TTCATCCAGC TGATATCAAT GGTAAAGCGG ATCCATATGT TGTCATAAAA
    CTAGGAAAAA CTGATATAAA AGATAAGGAA AACTACATCT CCAAGCAGCT AAACCCTGTC
    TTCGGAAAGT CCTTTGATAT TGAGGCCACT TTTCCGATGG AGTCCATGCT TACAGTTGCG
    GTTTATGACT GGGATCTGGT GGGGACGGAT GATCTGATCG GTGAAACCAA AATCGACCTG
    GAAAACCGTT ACTACAGCAA GCACAGGGCG ACTTGTGGTA TCTCAAAGAC ATATGCCATT
    CACGGGTACA ACTTGTGGAG GGACCCAGTG AAGCCTACAC AAATCTTATC CAAACTTTGC
    AAAGAAGGGA AGATCGATGG ACCTCATTAT GGCCCTGGTG GAAGAGTGAA AGTCACGAAT
    CGGGTATTCA CTGGTCCCAC AGAAATTGAT GATGAAAATG GTCAGAAGAA ACAAACGGAT
    GAGCACCTTG CACTCTCGGT CCTTCAGCAC TGGGAGGACA TTCCTCGGGT GGGATGTAAG
    CTGGTCCCAG AGCATGTAGA AACGAGGCCT TTGTTAAACC CAGACAAACC TGGAATTGAG
    CAGGGCCGCA TAGAAATGTG GGTAGACATG TTCCCCATGG ACATGCCTGC GCCAGGTCCT
    GCCATTGATA TCTCTCCAAG GAAGCCAAAA AGTTTTGAGC TACGTGTTAT TATTTGGAAC
    ACTGATGAAG TGATTCTAGA AGATGATGCT TTCATGACAG GAGAGAAGAT GTCTGACATC
    TATGTCAAGG GGTGGCTGAA GGGACAGCAG GAAGACAAAC AAGACACGGA TGTCCACTAT
    CATTCCCTTA CTGGGGAGGG GAACTTCAAC TGGAGGTTCT TGTTCCCTTT TGATTACCTT
    ATGGCAGAGG AGAAGATTGT CATCTCCAAG AAAGAGTCCA TGTTCTCTTG GGATGAGACC
    GAGTACAAGA TCCCGGCTCG TCTCACTCTC CAGGTCTGGG ATGCCGACCA CTTCTCAGCA
    GATGACTTTC TCGGAGCCAT AGAATTAGAC TTGAATCGCT TTCCCCGAGG GGCAAAAACA
    GCGAAACAGT GTTCGATAGA AATGGCGGAA CCCGACGTCG ATCTGCCAAT GGTCTCAATC
    TTCAAACAGA AGAGGGTGAA GGGATGGTGG CCTTTCGTTG CCAGGAATGA AAACGATGAG
    ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG CTTCACCTTC TCTCAGCAGA GGAGGCAGAG
    AAGACCCCGG CAGGGCTTGG GCGCAATGAA CCAGATCCTC TTGAAAAACC AAACCGCCCC
    GATACAAGTT TCATCTGGTT TCTGAACCCC CTGAAGTCTA TCCGCTATTT CATCTGGCAC
    ACTTATCGCT GGTTGATCCT GAAAATCCTC ATCCTCATCC TCCTTCTCCT CATGGTGGGG
    CTTTTCCTTT ACTCTTTGCC TGGATACCTG GTAAAGAAGA TCCTGGGAGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757742.1
    CDS97..4830
    Translation

    Target ORF information:

    RefSeq Version XM_031901882.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis otoferlin (otof), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901882.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
    GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG
    AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG
    TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG
    ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT
    TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG
    CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG
    AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT
    CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC
    CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT
    AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT
    ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT
    TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA
    ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC
    TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT
    ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG
    ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC
    TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG
    GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG
    ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT
    TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC
    ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC
    CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC
    CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG
    GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA
    TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC
    AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG
    AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC
    ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG
    TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA
    ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA
    ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG
    CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA
    GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA
    CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC
    AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA
    GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC
    CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG
    GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA
    GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT
    TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGCTAAACT CATGAATGGC
    TACTTGGCAA TGACCAATGG AAGGCCCCGC TCTCGCACCA CAGGTGAAAT AGTTATCAAC
    ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC
    GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG
    AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC
    AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC
    GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG AGATCAAAAC TGATGACTCT
    CCCATGAAAG GCAACAAGGG CCCCACGAAG AACAAGGAGA AGGTCAAAGC CCCAAAAGAG
    GAGAAGAAGA ACAAGAAGCA GCAACAGCAG CAGCAACAAG CCGATCAGGC CGAGAAGAAG
    AACAAGCAAA AGATCGATGA GTTAAAGGTG TACAACAAGG AGTTGGAGAC CGAGTATGAC
    AACTTTGAGG ACTGGCTCCA TACATTTAAC TTGTTACGAG GGAAGATTGG AGATGATGAC
    GATACTGTAA GCGAAGAAGA GCGAATTGTT GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC
    AAAGTTCCTT TGCCAGAAGA CGTCACAAAG GAAGCCGGGT ATGACCCAAA CTTCGGCATG
    TTCCAAGGAA TCCCTAACAA CGACCCAATG AATGTGCTGG TCAGAGTTTA TGTCGTGAGG
    GCCACCGATC TTCATCCAGC TGATATCAAT GGTAAAGCGG ATCCATATGT TGTCATAAAA
    CTAGGAAAAA CTGATATAAA AGATAAGGAA AACTACATCT CCAAGCAGCT AAACCCTGTC
    TTCGGAAAGT CCTTTGATAT TGAGGCCACT TTTCCGATGG AGTCCATGCT TACAGTTGCG
    GTTTATGACT GGGATCTGGT GGGGACGGAT GATCTGATCG GTGAAACCAA AATCGACCTG
    GAAAACCGTT ACTACAGCAA GCACAGGGCG ACTTGTGGTA TCTCAAAGAC ATATGCCATT
    CACGGGTACA ACTTGTGGAG GGACCCAGTG AAGCCTACAC AAATCTTATC CAAACTTTGC
    AAAGAAGGGA AGATCGATGG ACCTCATTAT GGCCCTGGTG GAAGAGTGAA AGTCACGAAT
    CGGGTATTCA CTGGTCCCAC AGAAATTGAT GATGAAAATG GTCAGAAGAA ACAAACGGAT
    GAGCACCTTG CACTCTCGGT CCTTCAGCAC TGGGAGGACA TTCCTCGGGT GGGATGTAAG
    CTGGTCCCAG AGCATGTAGA AACGAGGCCT TTGTTAAACC CAGACAAACC TGGAATTGAG
    CAGGGCCGCA TAGAAATGTG GGTAGACATG TTCCCCATGG ACATGCCTGC GCCAGGTCCT
    GCCATTGATA TCTCTCCAAG GAAGCCAAAA AGTTTTGAGC TACGTGTTAT TATTTGGAAC
    ACTGATGAAG TGATTCTAGA AGATGATGCT TTCATGACAG GAGAGAAGAT GTCTGACATC
    TATGTCAAGG GGTGGCTGAA GGGACAGCAG GAAGACAAAC AAGACACGGA TGTCCACTAT
    CATTCCCTTA CTGGGGAGGG GAACTTCAAC TGGAGGTTCT TGTTCCCTTT TGATTACCTT
    ATGGCAGAGG AGAAGATTGT CATCTCCAAG AAAGAGTCCA TGTTCTCTTG GGATGAGACC
    GAGTACAAGA TCCCGGCTCG TCTCACTCTC CAGGTCTGGG ATGCCGACCA CTTCTCAGCA
    GATGACTTTC TCGGAGCCAT AGAATTAGAC TTGAATCGCT TTCCCCGAGG GGCAAAAACA
    GCGAAACAGT GTTCGATAGA AATGGCGGAA CCCGACGTCG ATCTGCCAAT GGTCTCAATC
    TTCAAACAGA AGAGGGTGAA GGGATGGTGG CCTTTCGTTG CCAGGAATGA AAACGATGAG
    ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG CTTCACCTTC TCTCAGCAGA GGAGGCAGAG
    AAGACCCCGG CAGGGCTTGG GCGCAATGAA CCAGATCCTC TTGAAAAACC AAACCGCCCC
    GATACAAGTT TCATCTGGTT TCTGAACCCC CTGAAGTCTA TCCGCTATTT CATCTGGCAC
    ACTTATCGCT GGTTGATCCT GAAAATCCTC ATCCTCATCC TCCTTCTCCT CATGGTGGGG
    CTTTTCCTTT ACTCTTTGCC TGGATACCTG GTAAAGAAGA TCCTGGGAGC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58171
    Clone ID Related Accession (Same CDS sequence) XM_031901883.1
    Accession Version XM_031901883.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product otoferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
    GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG
    AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG
    TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG
    ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT
    TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG
    CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG
    AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT
    CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC
    CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT
    AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT
    ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT
    TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA
    ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC
    TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT
    ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG
    ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC
    TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG
    GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG
    ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT
    TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC
    ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC
    CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC
    CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG
    GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA
    TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC
    AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG
    AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC
    ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG
    TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA
    ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA
    ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG
    CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA
    GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA
    CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC
    AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA
    GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC
    CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG
    GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA
    GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT
    TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGCTAAACT CATGAATGGC
    TACTTGGCAA TGACCAATGG AAGGCCCCGC TCTCGCACCA CAGGTGAAAT AGTTATCAAC
    ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC
    GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG
    AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC
    AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC
    GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG GCAACAAGGG CCCCACGAAG
    AACAAGGAGA AGGTCAAAGC CCCAAAAGAG GAGAAGAAGA ACAAGAAGCA GCAACAGCAG
    CAGCAACAAG CCGATCAGGC CGAGAAGAAG AACAAGCAAA AGATCGATGA GTTAAAGGTG
    TACAACAAGG AGTTGGAGAC CGAGTATGAC AACTTTGAGG ACTGGCTCCA TACATTTAAC
    TTGTTACGAG GGAAGATTGG AGATGATGAC GATACTGTAA GCGAAGAAGA GCGAATTGTT
    GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC AAAGTTCCTT TGCCAGAAGA CGTCACAAAG
    GAAGCCGGGT ATGACCCAAA CTTCGGCATG TTCCAAGGAA TCCCTAACAA CGACCCAATG
    AATGTGCTGG TCAGAGTTTA TGTCGTGAGG GCCACCGATC TTCATCCAGC TGATATCAAT
    GGTAAAGCGG ATCCATATGT TGTCATAAAA CTAGGAAAAA CTGATATAAA AGATAAGGAA
    AACTACATCT CCAAGCAGCT AAACCCTGTC TTCGGAAAGT CCTTTGATAT TGAGGCCACT
    TTTCCGATGG AGTCCATGCT TACAGTTGCG GTTTATGACT GGGATCTGGT GGGGACGGAT
    GATCTGATCG GTGAAACCAA AATCGACCTG GAAAACCGTT ACTACAGCAA GCACAGGGCG
    ACTTGTGGTA TCTCAAAGAC ATATGCCATT CACGGGTACA ACTTGTGGAG GGACCCAGTG
    AAGCCTACAC AAATCTTATC CAAACTTTGC AAAGAAGGGA AGATCGATGG ACCTCATTAT
    GGCCCTGGTG GAAGAGTGAA AGTCACGAAT CGGGTATTCA CTGGTCCCAC AGAAATTGAT
    GATGAAAATG GTCAGAAGAA ACAAACGGAT GAGCACCTTG CACTCTCGGT CCTTCAGCAC
    TGGGAGGACA TTCCTCGGGT GGGATGTAAG CTGGTCCCAG AGCATGTAGA AACGAGGCCT
    TTGTTAAACC CAGACAAACC TGGAATTGAG CAGGGCCGCA TAGAAATGTG GGTAGACATG
    TTCCCCATGG ACATGCCTGC GCCAGGTCCT GCCATTGATA TCTCTCCAAG GAAGCCAAAA
    AGATACGAGC TTAGAGTGAT AATATGGAAT ACAGATGAGG TAATCCTGGA AGATGATGAT
    TACTTCACAG GAGAAAAATC TAGTGACATA TTTGTCAGGG GGTGGCTGAA GGGACAGCAG
    GAAGACAAAC AAGACACGGA TGTCCACTAT CATTCCCTTA CTGGGGAGGG GAACTTCAAC
    TGGAGGTTCT TGTTCCCTTT TGATTACCTT ATGGCAGAGG AGAAGATTGT CATCTCCAAG
    AAAGAGTCCA TGTTCTCTTG GGATGAGACC GAGTACAAGA TCCCGGCTCG TCTCACTCTC
    CAGGTCTGGG ATGCCGACCA CTTCTCAGCA GATGACTTTC TCGGAGCCAT AGAATTAGAC
    TTGAATCGCT TTCCCCGAGG GGCAAAAACA GCGAAACAGT GTTCGATAGA AATGGCGGAA
    CCCGACGTCG ATCTGCCAAT GGTCTCAATC TTCAAACAGA AGAGGGTGAA GGGATGGTGG
    CCTTTCGTTG CCAGGAATGA AAACGATGAG ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG
    CTTCACCTTC TCTCAGCAGA GGAGGCAGAG AAGACCCCGG CAGGGCTTGG GCGCAATGAA
    CCAGATCCTC TTGAAAAACC AAATCGGCCG AATCCCAGCC TGGCGTGGAT GCTGAGCCCC
    CTGCGGGTAG TGTGCATGTT GCTATGGCGA CAGTACCGGT GGCACGTACT GGGCATCATC
    GGTTCCTGTG CGGTTCTGAT GTTCGTGGCG TTGGTGCTCT ACACCCTCCC CGGCGCTATC
    AGCGAGAGGC TCATTAACCC CCGCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757743.1
    CDS97..4806
    Translation

    Target ORF information:

    RefSeq Version XM_031901883.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis otoferlin (otof), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901883.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
    GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG
    AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG
    TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG
    ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT
    TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG
    CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG
    AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT
    CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC
    CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT
    AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT
    ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT
    TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA
    ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC
    TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT
    ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG
    ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC
    TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG
    GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG
    ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT
    TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC
    ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC
    CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC
    CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG
    GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA
    TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC
    AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG
    AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC
    ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG
    TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA
    ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA
    ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG
    CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA
    GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA
    CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC
    AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA
    GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC
    CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG
    GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA
    GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT
    TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGCTAAACT CATGAATGGC
    TACTTGGCAA TGACCAATGG AAGGCCCCGC TCTCGCACCA CAGGTGAAAT AGTTATCAAC
    ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC
    GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG
    AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC
    AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC
    GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG GCAACAAGGG CCCCACGAAG
    AACAAGGAGA AGGTCAAAGC CCCAAAAGAG GAGAAGAAGA ACAAGAAGCA GCAACAGCAG
    CAGCAACAAG CCGATCAGGC CGAGAAGAAG AACAAGCAAA AGATCGATGA GTTAAAGGTG
    TACAACAAGG AGTTGGAGAC CGAGTATGAC AACTTTGAGG ACTGGCTCCA TACATTTAAC
    TTGTTACGAG GGAAGATTGG AGATGATGAC GATACTGTAA GCGAAGAAGA GCGAATTGTT
    GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC AAAGTTCCTT TGCCAGAAGA CGTCACAAAG
    GAAGCCGGGT ATGACCCAAA CTTCGGCATG TTCCAAGGAA TCCCTAACAA CGACCCAATG
    AATGTGCTGG TCAGAGTTTA TGTCGTGAGG GCCACCGATC TTCATCCAGC TGATATCAAT
    GGTAAAGCGG ATCCATATGT TGTCATAAAA CTAGGAAAAA CTGATATAAA AGATAAGGAA
    AACTACATCT CCAAGCAGCT AAACCCTGTC TTCGGAAAGT CCTTTGATAT TGAGGCCACT
    TTTCCGATGG AGTCCATGCT TACAGTTGCG GTTTATGACT GGGATCTGGT GGGGACGGAT
    GATCTGATCG GTGAAACCAA AATCGACCTG GAAAACCGTT ACTACAGCAA GCACAGGGCG
    ACTTGTGGTA TCTCAAAGAC ATATGCCATT CACGGGTACA ACTTGTGGAG GGACCCAGTG
    AAGCCTACAC AAATCTTATC CAAACTTTGC AAAGAAGGGA AGATCGATGG ACCTCATTAT
    GGCCCTGGTG GAAGAGTGAA AGTCACGAAT CGGGTATTCA CTGGTCCCAC AGAAATTGAT
    GATGAAAATG GTCAGAAGAA ACAAACGGAT GAGCACCTTG CACTCTCGGT CCTTCAGCAC
    TGGGAGGACA TTCCTCGGGT GGGATGTAAG CTGGTCCCAG AGCATGTAGA AACGAGGCCT
    TTGTTAAACC CAGACAAACC TGGAATTGAG CAGGGCCGCA TAGAAATGTG GGTAGACATG
    TTCCCCATGG ACATGCCTGC GCCAGGTCCT GCCATTGATA TCTCTCCAAG GAAGCCAAAA
    AGATACGAGC TTAGAGTGAT AATATGGAAT ACAGATGAGG TAATCCTGGA AGATGATGAT
    TACTTCACAG GAGAAAAATC TAGTGACATA TTTGTCAGGG GGTGGCTGAA GGGACAGCAG
    GAAGACAAAC AAGACACGGA TGTCCACTAT CATTCCCTTA CTGGGGAGGG GAACTTCAAC
    TGGAGGTTCT TGTTCCCTTT TGATTACCTT ATGGCAGAGG AGAAGATTGT CATCTCCAAG
    AAAGAGTCCA TGTTCTCTTG GGATGAGACC GAGTACAAGA TCCCGGCTCG TCTCACTCTC
    CAGGTCTGGG ATGCCGACCA CTTCTCAGCA GATGACTTTC TCGGAGCCAT AGAATTAGAC
    TTGAATCGCT TTCCCCGAGG GGCAAAAACA GCGAAACAGT GTTCGATAGA AATGGCGGAA
    CCCGACGTCG ATCTGCCAAT GGTCTCAATC TTCAAACAGA AGAGGGTGAA GGGATGGTGG
    CCTTTCGTTG CCAGGAATGA AAACGATGAG ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG
    CTTCACCTTC TCTCAGCAGA GGAGGCAGAG AAGACCCCGG CAGGGCTTGG GCGCAATGAA
    CCAGATCCTC TTGAAAAACC AAATCGGCCG AATCCCAGCC TGGCGTGGAT GCTGAGCCCC
    CTGCGGGTAG TGTGCATGTT GCTATGGCGA CAGTACCGGT GGCACGTACT GGGCATCATC
    GGTTCCTGTG CGGTTCTGAT GTTCGTGGCG TTGGTGCTCT ACACCCTCCC CGGCGCTATC
    AGCGAGAGGC TCATTAACCC CCGCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58172
    Clone ID Related Accession (Same CDS sequence) XM_031901884.1
    Accession Version XM_031901884.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product otoferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
    GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG
    AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG
    TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG
    ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT
    TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG
    CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG
    AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT
    CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC
    CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT
    AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT
    ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT
    TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA
    ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC
    TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT
    ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG
    ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC
    TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG
    GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG
    ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT
    TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC
    ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC
    CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC
    CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG
    GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA
    TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC
    AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG
    AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC
    ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG
    TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA
    ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA
    ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG
    CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA
    GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA
    CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC
    AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA
    GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC
    CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG
    GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA
    GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT
    TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGGTGAAAT AGTTATCAAC
    ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC
    GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG
    AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC
    AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC
    GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG AGATCAAAAC TGATGACTCT
    CCCATGAAAG GCAACAAGGG CCCCACGAAG AACAAGGAGA AGGTCAAAGC CCCAAAAGAG
    GAGAAGAAGA ACAAGAAGCA GCAACAGCAG CAGCAACAAG CCGATCAGGC CGAGAAGAAG
    AACAAGCAAA AGATCGATGA GTTAAAGGTG TACAACAAGG AGTTGGAGAC CGAGTATGAC
    AACTTTGAGG ACTGGCTCCA TACATTTAAC TTGTTACGAG GGAAGATTGG AGATGATGAC
    GATACTGTAA GCGAAGAAGA GCGAATTGTT GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC
    AAAGTTCCTT TGCCAGAAGA CGTCACAAAG GAAGCCGGGT ATGACCCAAA CTTCGGCATG
    TTCCAAGGAA TCCCTAACAA CGACCCAATG AATGTGCTGG TCAGAGTTTA TGTCGTGAGG
    GCCACCGATC TTCATCCAGC TGATATCAAT GGTAAAGCGG ATCCATATGT TGTCATAAAA
    CTAGGAAAAA CTGATATAAA AGATAAGGAA AACTACATCT CCAAGCAGCT AAACCCTGTC
    TTCGGAAAGT CCTTTGATAT TGAGGCCACT TTTCCGATGG AGTCCATGCT TACAGTTGCG
    GTTTATGACT GGGATCTGGT GGGGACGGAT GATCTGATCG GTGAAACCAA AATCGACCTG
    GAAAACCGTT ACTACAGCAA GCACAGGGCG ACTTGTGGTA TCTCAAAGAC ATATGCCATT
    CACGGGTACA ACTTGTGGAG GGACCCAGTG AAGCCTACAC AAATCTTATC CAAACTTTGC
    AAAGAAGGGA AGATCGATGG ACCTCATTAT GGCCCTGGTG GAAGAGTGAA AGTCACGAAT
    CGGGTATTCA CTGGTCCCAC AGAAATTGAT GATGAAAATG GTCAGAAGAA ACAAACGGAT
    GAGCACCTTG CACTCTCGGT CCTTCAGCAC TGGGAGGACA TTCCTCGGGT GGGATGTAAG
    CTGGTCCCAG AGCATGTAGA AACGAGGCCT TTGTTAAACC CAGACAAACC TGGAATTGAG
    CAGGGCCGCA TAGAAATGTG GGTAGACATG TTCCCCATGG ACATGCCTGC GCCAGGTCCT
    GCCATTGATA TCTCTCCAAG GAAGCCAAAA AGATACGAGC TTAGAGTGAT AATATGGAAT
    ACAGATGAGG TAATCCTGGA AGATGATGAT TACTTCACAG GAGAAAAATC TAGTGACATA
    TTTGTCAGGG GGTGGCTGAA GGGACAGCAG GAAGACAAAC AAGACACGGA TGTCCACTAT
    CATTCCCTTA CTGGGGAGGG GAACTTCAAC TGGAGGTTCT TGTTCCCTTT TGATTACCTT
    ATGGCAGAGG AGAAGATTGT CATCTCCAAG AAAGAGTCCA TGTTCTCTTG GGATGAGACC
    GAGTACAAGA TCCCGGCTCG TCTCACTCTC CAGGTCTGGG ATGCCGACCA CTTCTCAGCA
    GATGACTTTC TCGGAGCCAT AGAATTAGAC TTGAATCGCT TTCCCCGAGG GGCAAAAACA
    GCGAAACAGT GTTCGATAGA AATGGCGGAA CCCGACGTCG ATCTGCCAAT GGTCTCAATC
    TTCAAACAGA AGAGGGTGAA GGGATGGTGG CCTTTCGTTG CCAGGAATGA AAACGATGAG
    ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG CTTCACCTTC TCTCAGCAGA GGAGGCAGAG
    AAGACCCCGG CAGGGCTTGG GCGCAATGAA CCAGATCCTC TTGAAAAACC AAATCGGCCG
    AATCCCAGCC TGGCGTGGAT GCTGAGCCCC CTGCGGGTAG TGTGCATGTT GCTATGGCGA
    CAGTACCGGT GGCACGTACT GGGCATCATC GGTTCCTGTG CGGTTCTGAT GTTCGTGGCG
    TTGGTGCTCT ACACCCTCCC CGGCGCTATC AGCGAGAGGC TCATTAACCC CCGCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757744.1
    CDS97..4776
    Translation

    Target ORF information:

    RefSeq Version XM_031901884.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis otoferlin (otof), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901884.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
    GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG
    AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG
    TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG
    ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT
    TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG
    CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG
    AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT
    CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC
    CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT
    AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT
    ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT
    TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA
    ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC
    TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT
    ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG
    ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC
    TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG
    GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG
    ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT
    TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC
    ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC
    CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC
    CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG
    GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA
    TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC
    AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG
    AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC
    ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG
    TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA
    ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA
    ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG
    CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA
    GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA
    CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC
    AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA
    GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC
    CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG
    GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA
    GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT
    TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGGTGAAAT AGTTATCAAC
    ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC
    GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG
    AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC
    AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC
    GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG AGATCAAAAC TGATGACTCT
    CCCATGAAAG GCAACAAGGG CCCCACGAAG AACAAGGAGA AGGTCAAAGC CCCAAAAGAG
    GAGAAGAAGA ACAAGAAGCA GCAACAGCAG CAGCAACAAG CCGATCAGGC CGAGAAGAAG
    AACAAGCAAA AGATCGATGA GTTAAAGGTG TACAACAAGG AGTTGGAGAC CGAGTATGAC
    AACTTTGAGG ACTGGCTCCA TACATTTAAC TTGTTACGAG GGAAGATTGG AGATGATGAC
    GATACTGTAA GCGAAGAAGA GCGAATTGTT GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC
    AAAGTTCCTT TGCCAGAAGA CGTCACAAAG GAAGCCGGGT ATGACCCAAA CTTCGGCATG
    TTCCAAGGAA TCCCTAACAA CGACCCAATG AATGTGCTGG TCAGAGTTTA TGTCGTGAGG
    GCCACCGATC TTCATCCAGC TGATATCAAT GGTAAAGCGG ATCCATATGT TGTCATAAAA
    CTAGGAAAAA CTGATATAAA AGATAAGGAA AACTACATCT CCAAGCAGCT AAACCCTGTC
    TTCGGAAAGT CCTTTGATAT TGAGGCCACT TTTCCGATGG AGTCCATGCT TACAGTTGCG
    GTTTATGACT GGGATCTGGT GGGGACGGAT GATCTGATCG GTGAAACCAA AATCGACCTG
    GAAAACCGTT ACTACAGCAA GCACAGGGCG ACTTGTGGTA TCTCAAAGAC ATATGCCATT
    CACGGGTACA ACTTGTGGAG GGACCCAGTG AAGCCTACAC AAATCTTATC CAAACTTTGC
    AAAGAAGGGA AGATCGATGG ACCTCATTAT GGCCCTGGTG GAAGAGTGAA AGTCACGAAT
    CGGGTATTCA CTGGTCCCAC AGAAATTGAT GATGAAAATG GTCAGAAGAA ACAAACGGAT
    GAGCACCTTG CACTCTCGGT CCTTCAGCAC TGGGAGGACA TTCCTCGGGT GGGATGTAAG
    CTGGTCCCAG AGCATGTAGA AACGAGGCCT TTGTTAAACC CAGACAAACC TGGAATTGAG
    CAGGGCCGCA TAGAAATGTG GGTAGACATG TTCCCCATGG ACATGCCTGC GCCAGGTCCT
    GCCATTGATA TCTCTCCAAG GAAGCCAAAA AGATACGAGC TTAGAGTGAT AATATGGAAT
    ACAGATGAGG TAATCCTGGA AGATGATGAT TACTTCACAG GAGAAAAATC TAGTGACATA
    TTTGTCAGGG GGTGGCTGAA GGGACAGCAG GAAGACAAAC AAGACACGGA TGTCCACTAT
    CATTCCCTTA CTGGGGAGGG GAACTTCAAC TGGAGGTTCT TGTTCCCTTT TGATTACCTT
    ATGGCAGAGG AGAAGATTGT CATCTCCAAG AAAGAGTCCA TGTTCTCTTG GGATGAGACC
    GAGTACAAGA TCCCGGCTCG TCTCACTCTC CAGGTCTGGG ATGCCGACCA CTTCTCAGCA
    GATGACTTTC TCGGAGCCAT AGAATTAGAC TTGAATCGCT TTCCCCGAGG GGCAAAAACA
    GCGAAACAGT GTTCGATAGA AATGGCGGAA CCCGACGTCG ATCTGCCAAT GGTCTCAATC
    TTCAAACAGA AGAGGGTGAA GGGATGGTGG CCTTTCGTTG CCAGGAATGA AAACGATGAG
    ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG CTTCACCTTC TCTCAGCAGA GGAGGCAGAG
    AAGACCCCGG CAGGGCTTGG GCGCAATGAA CCAGATCCTC TTGAAAAACC AAATCGGCCG
    AATCCCAGCC TGGCGTGGAT GCTGAGCCCC CTGCGGGTAG TGTGCATGTT GCTATGGCGA
    CAGTACCGGT GGCACGTACT GGGCATCATC GGTTCCTGTG CGGTTCTGAT GTTCGTGGCG
    TTGGTGCTCT ACACCCTCCC CGGCGCTATC AGCGAGAGGC TCATTAACCC CCGCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa58173
    Clone ID Related Accession (Same CDS sequence) XM_031901885.1
    Accession Version XM_031901885.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product otoferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
    GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG
    AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG
    TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG
    ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT
    TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG
    CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG
    AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT
    CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC
    CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT
    AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT
    ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT
    TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA
    ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC
    TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT
    ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG
    ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC
    TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG
    GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG
    ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT
    TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC
    ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC
    CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC
    CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG
    GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA
    TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC
    AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG
    AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC
    ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG
    TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA
    ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA
    ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG
    CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA
    GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA
    CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC
    AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA
    GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC
    CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG
    GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA
    GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT
    TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGGTGAAAT AGTTATCAAC
    ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC
    GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG
    AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC
    AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC
    GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG GCAACAAGGG CCCCACGAAG
    AACAAGGAGA AGGTCAAAGC CCCAAAAGAG GAGAAGAAGA ACAAGAAGCA GCAACAGCAG
    CAGCAACAAG CCGATCAGGC CGAGAAGAAG AACAAGCAAA AGATCGATGA GTTAAAGGTG
    TACAACAAGG AGTTGGAGAC CGAGTATGAC AACTTTGAGG ACTGGCTCCA TACATTTAAC
    TTGTTACGAG GGAAGATTGG AGATGATGAC GATACTGTAA GCGAAGAAGA GCGAATTGTT
    GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC AAAGTTCCTT TGCCAGAAGA CGTCACAAAG
    GAAGCCGGGT ATGACCCAAA CTTCGGCATG TTCCAAGGAA TCCCTAACAA CGACCCAATG
    AATGTGCTGG TCAGAGTTTA TGTCGTGAGG GCCACCGATC TTCATCCAGC TGATATCAAT
    GGTAAAGCGG ATCCATATGT TGTCATAAAA CTAGGAAAAA CTGATATAAA AGATAAGGAA
    AACTACATCT CCAAGCAGCT AAACCCTGTC TTCGGAAAGT CCTTTGATAT TGAGGCCACT
    TTTCCGATGG AGTCCATGCT TACAGTTGCG GTTTATGACT GGGATCTGGT GGGGACGGAT
    GATCTGATCG GTGAAACCAA AATCGACCTG GAAAACCGTT ACTACAGCAA GCACAGGGCG
    ACTTGTGGTA TCTCAAAGAC ATATGCCATT CACGGGTACA ACTTGTGGAG GGACCCAGTG
    AAGCCTACAC AAATCTTATC CAAACTTTGC AAAGAAGGGA AGATCGATGG ACCTCATTAT
    GGCCCTGGTG GAAGAGTGAA AGTCACGAAT CGGGTATTCA CTGGTCCCAC AGAAATTGAT
    GATGAAAATG GTCAGAAGAA ACAAACGGAT GAGCACCTTG CACTCTCGGT CCTTCAGCAC
    TGGGAGGACA TTCCTCGGGT GGGATGTAAG CTGGTCCCAG AGCATGTAGA AACGAGGCCT
    TTGTTAAACC CAGACAAACC TGGAATTGAG CAGGGCCGCA TAGAAATGTG GGTAGACATG
    TTCCCCATGG ACATGCCTGC GCCAGGTCCT GCCATTGATA TCTCTCCAAG GAAGCCAAAA
    AGATACGAGC TTAGAGTGAT AATATGGAAT ACAGATGAGG TAATCCTGGA AGATGATGAT
    TACTTCACAG GAGAAAAATC TAGTGACATA TTTGTCAGGG GGTGGCTGAA GGGACAGCAG
    GAAGACAAAC AAGACACGGA TGTCCACTAT CATTCCCTTA CTGGGGAGGG GAACTTCAAC
    TGGAGGTTCT TGTTCCCTTT TGATTACCTT ATGGCAGAGG AGAAGATTGT CATCTCCAAG
    AAAGAGTCCA TGTTCTCTTG GGATGAGACC GAGTACAAGA TCCCGGCTCG TCTCACTCTC
    CAGGTCTGGG ATGCCGACCA CTTCTCAGCA GATGACTTTC TCGGAGCCAT AGAATTAGAC
    TTGAATCGCT TTCCCCGAGG GGCAAAAACA GCGAAACAGT GTTCGATAGA AATGGCGGAA
    CCCGACGTCG ATCTGCCAAT GGTCTCAATC TTCAAACAGA AGAGGGTGAA GGGATGGTGG
    CCTTTCGTTG CCAGGAATGA AAACGATGAG ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG
    CTTCACCTTC TCTCAGCAGA GGAGGCAGAG AAGACCCCGG CAGGGCTTGG GCGCAATGAA
    CCAGATCCTC TTGAAAAACC AAATCGACCC GACACCTCGT TCATCTGGTT CCTCAACCCA
    CTTAAGTCCA TCAAGTATCT CATCTGTAAT CGCTACAAGT GGCTCATCAT TAAGATTGTG
    CTGGCGTTGC TGCTTTTGGC CATGGTGGCC CTCTTCCTCT ACAGCATGCC CGGTTACATG
    GTGAAGAAGC TTCTGGGTGC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757745.1
    CDS97..4740
    Translation

    Target ORF information:

    RefSeq Version XM_031901885.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis otoferlin (otof), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901885.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACACCA GCATCATGGC CAACGTCAAG AAAGCTCTCA TTGGAGAAAA CAAGGACTTG 
    GTGGACCCCT ATGTGCAAGT TGCCTTTGCA GGGCAGAAGG GAAAGACCTC GGTGCAGAAG
    AGCAGCTACG AGCCCATGTG GAACGAGCAG ATCATTTTCA CCGAGATGTT CCCCCCCCTG
    TGCAAGAGGA TGAAAGTGCA GATCAGAGAT TCCGACAAGG TCAACGATGT CGCCATTGGG
    ACCCACTTCA TTGACCTGAG GAAAATTGCC AACGAGGGGG ACAAGGGATT CCTCCCTACT
    TTCGGCCCAG CCTGGGTGAA TATGTATGGC TCCACCCGTA ACTACACCCT CATGGATGAG
    CACCAGGATC TGAATGAGGG GCTCGGAGAA GGCGTCTCTT TCCGCGCAAG GCTGCTGCTG
    AGTCTCGCTG TTGAGATACT GGATACAACC AACCCAGAGC TCACCAGTTC CACAGAGGTT
    CAAGTTGAAG GAGCTCAACC AGTGGCAGAA ACCTGTACCG GTAAAATGGA AGAGTTCTTC
    CTGTTTGGAG CATTCCTGGA ATCCACCATG ATTGATCGGA AAATTGGGGA CAAGCCAATT
    AACTTTGAGG TCACCATAGG TAACTATGGC AACCAAATAG ATGGAATTTC CAAGCCGAGT
    ATGAGGAAGA GGAAGGAAGG AGGTGACGGA GTCGAGGAGG AGACGGAGCT CTTGCAGAAT
    TCCAGTGATG ATGAGGCTGA TGAAGATGGA GAAATGTTAT CAATCTCTTC GTCCCCTGCA
    ATGAAGCCAC TCATAACTGA TAGGAACTAC TTCCACCTCC CGTTCTTTGA TAAGAAGCCC
    TGTACCTACA TTAAGAGTTG GTGGCAGGAC CAGCGGAGGC GGCTGTACAA TGCCAACATT
    ATGGACAAGA TCGCAGATAA GCTGGAGGAA GGCTTGAACG ATGTTCATGA GATGATAAAG
    ACGGAGAAGC CCTACCCTGA ACGGCGGCTG CGAGGAGTCC TGGAGGAGCT GAGTAGCAGC
    TGTTTTCGAT TTGTTACCTT GGCCAACAAG GACCAGAACC AGAATGGGCA GACGAAACTG
    GACAGGGAGA GGCTGAAGTC CTGCATGAGG GAGCTGGAGA ACATGGGGAC ACAAGCCAAG
    ACCATGAGGT CCCAAGTGAA GAAGAACACG GTTAAGGACA AGCTGAAACT GGTGCAGAAT
    TTCCTGCAGA AGCTCCGGTT CCTGGCAGAT GAGCCCCAGC ACAGCATTCC TGACATCTTC
    ATCTGGATGA TGTCCAACAA CAAGCGCATC GCCTACGCTC GAATCCCTTC CAAAGATATC
    CTGTACTCCA TTGTGGATGA GGAGACGGGA AAGGACTGTG GCAAAGTCAA GTGTGTCTTC
    CTTAAGCTGC CGGGCAAGAG GGGCTTTGGG CCTGCAGGCT GGACGGTACA AGCCAAGATG
    GAGGTCTACA TGTGGTTGGG CCTTAGCAAG CAGCGCAGGG ATTTCTTGTG TGGTCTTCCA
    TGTGGATTTG AGGAGATAAA AGCAGTGAAA GGGCCCGGTC TGCAGTCCTT CCCACCCATC
    AGCCTCAGCT ACACCAAAAA ACAAGTGTTC CAGTTACGAG CCCACATGTA CCAAGCCCGG
    AGTCTCTTTG CCGCTGACAG CAGTGGACTT TCCGACCCCT TTGCCCGCGT CTTCTTCAAC
    ACTCAGAGCC AATGTACAGA GGTGCTGAAT GAGACCCTTT GTCCGACTTG GGACCAGCTG
    TTGGTGTTTG ACAATATCGA ACTCTACGGA GAAGCTCACG AGATGAGAGA CGACCCCCCA
    ATCATTGTCA TTGAGATTTA TGATCAAGAC ACAGTAGGGA AGGCCGATTT CATGGGGAGA
    ACATTTGCCA AACCTGTTGT GAAAATGTCA GATGAGCGGT ACTGCCCTCC CCGCTTTCCG
    CCACAGCTGG AATACTACCA GATCTACCGT GGGAACTCAA CCGCAGGGGA CCTCTTGGGA
    GCTTTCGAAC TTCTCCAGAT CGGACCAGCG GGCAAGTCTG ACCTCCCACA GATTGAAGGA
    CCAACAGACA TAGAGAGAGG ACCCATCCTT CCAGTTCCTC TTGGAATCAG ACCAGTGCTC
    AGCAAATACA GAGTAGAGGT CCTTTTCTGG GGGCTGAGAG ACCTGAAGAG AGTAAATCTA
    GCCCAGGTTG ATAGACCAAG GGTGGATATT GAGTGTGCCG GGAGGGGGGT GCAGTCTGCC
    CTCATCCAGA ACTACAAGAA GAATCCAAAT TTTAGCACGT TGGTCAAATG GTTTGAAGTG
    GATCTCCCCG AGAATGAGTT ACTTCACCCG CCATTGAATA TCCGAGTAGT AGACTGCCGA
    GCCTTTGGCA GATACACTCT GGTTGGTTCC CATGCTGTCA GCTCCCTACG CAGGTTCATT
    TACCGGCCTC CGGATAAGAA CTCCCAGCAG TGGACCACAG CAGGTGAAAT AGTTATCAAC
    ATGGAGCCCG AAGCGCCCGT CAAGAAGATG GAGACAATGG TGAAACTGGA AGCCAATTCC
    GACGCGGTGG TTAAAGTAGA CGTGAGCGAG GAAGAAAAAG ACAAGAAGAA GAAGAAGAAG
    AAAGGAGTAG AGGAGATAGA GGAAGAGGAG CCAGATGAGA GCATGTTGGA CTGGTGGTCC
    AAATACTTTG CTTCCATTGA AACCATGAAG GAGCAACTGC GCCAACAGGA AGCGGCAGCC
    GCCGAAGCCG AAGAGAAGGA AGAGATGGAG ATCGCAGAGG GCAACAAGGG CCCCACGAAG
    AACAAGGAGA AGGTCAAAGC CCCAAAAGAG GAGAAGAAGA ACAAGAAGCA GCAACAGCAG
    CAGCAACAAG CCGATCAGGC CGAGAAGAAG AACAAGCAAA AGATCGATGA GTTAAAGGTG
    TACAACAAGG AGTTGGAGAC CGAGTATGAC AACTTTGAGG ACTGGCTCCA TACATTTAAC
    TTGTTACGAG GGAAGATTGG AGATGATGAC GATACTGTAA GCGAAGAAGA GCGAATTGTT
    GGGAGGTTTA AGGGTTCCAT GTGTGTGTAC AAAGTTCCTT TGCCAGAAGA CGTCACAAAG
    GAAGCCGGGT ATGACCCAAA CTTCGGCATG TTCCAAGGAA TCCCTAACAA CGACCCAATG
    AATGTGCTGG TCAGAGTTTA TGTCGTGAGG GCCACCGATC TTCATCCAGC TGATATCAAT
    GGTAAAGCGG ATCCATATGT TGTCATAAAA CTAGGAAAAA CTGATATAAA AGATAAGGAA
    AACTACATCT CCAAGCAGCT AAACCCTGTC TTCGGAAAGT CCTTTGATAT TGAGGCCACT
    TTTCCGATGG AGTCCATGCT TACAGTTGCG GTTTATGACT GGGATCTGGT GGGGACGGAT
    GATCTGATCG GTGAAACCAA AATCGACCTG GAAAACCGTT ACTACAGCAA GCACAGGGCG
    ACTTGTGGTA TCTCAAAGAC ATATGCCATT CACGGGTACA ACTTGTGGAG GGACCCAGTG
    AAGCCTACAC AAATCTTATC CAAACTTTGC AAAGAAGGGA AGATCGATGG ACCTCATTAT
    GGCCCTGGTG GAAGAGTGAA AGTCACGAAT CGGGTATTCA CTGGTCCCAC AGAAATTGAT
    GATGAAAATG GTCAGAAGAA ACAAACGGAT GAGCACCTTG CACTCTCGGT CCTTCAGCAC
    TGGGAGGACA TTCCTCGGGT GGGATGTAAG CTGGTCCCAG AGCATGTAGA AACGAGGCCT
    TTGTTAAACC CAGACAAACC TGGAATTGAG CAGGGCCGCA TAGAAATGTG GGTAGACATG
    TTCCCCATGG ACATGCCTGC GCCAGGTCCT GCCATTGATA TCTCTCCAAG GAAGCCAAAA
    AGATACGAGC TTAGAGTGAT AATATGGAAT ACAGATGAGG TAATCCTGGA AGATGATGAT
    TACTTCACAG GAGAAAAATC TAGTGACATA TTTGTCAGGG GGTGGCTGAA GGGACAGCAG
    GAAGACAAAC AAGACACGGA TGTCCACTAT CATTCCCTTA CTGGGGAGGG GAACTTCAAC
    TGGAGGTTCT TGTTCCCTTT TGATTACCTT ATGGCAGAGG AGAAGATTGT CATCTCCAAG
    AAAGAGTCCA TGTTCTCTTG GGATGAGACC GAGTACAAGA TCCCGGCTCG TCTCACTCTC
    CAGGTCTGGG ATGCCGACCA CTTCTCAGCA GATGACTTTC TCGGAGCCAT AGAATTAGAC
    TTGAATCGCT TTCCCCGAGG GGCAAAAACA GCGAAACAGT GTTCGATAGA AATGGCGGAA
    CCCGACGTCG ATCTGCCAAT GGTCTCAATC TTCAAACAGA AGAGGGTGAA GGGATGGTGG
    CCTTTCGTTG CCAGGAATGA AAACGATGAG ATGGAGCTGA CTGGTAAAGT GGAGGCGGAG
    CTTCACCTTC TCTCAGCAGA GGAGGCAGAG AAGACCCCGG CAGGGCTTGG GCGCAATGAA
    CCAGATCCTC TTGAAAAACC AAATCGACCC GACACCTCGT TCATCTGGTT CCTCAACCCA
    CTTAAGTCCA TCAAGTATCT CATCTGTAAT CGCTACAAGT GGCTCATCAT TAAGATTGTG
    CTGGCGTTGC TGCTTTTGGC CATGGTGGCC CTCTTCCTCT ACAGCATGCC CGGTTACATG
    GTGAAGAAGC TTCTGGGTGC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa30655
    Clone ID Related Accession (Same CDS sequence) XM_012971322.1
    Accession Version XM_012971322.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6063bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product otoferlin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016683731.1) and transcript sequence (BC136162.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 360 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCCCTAA TAGTGCATCT CAGGACTGTC ACCGACCTGC GGGGGAAAGG GGACCGGATC 
    GCCAAAGTGA CGTTCAGAGG ACTGTCGTTC TACACGCGGG TGTTGGAGAA CTGTGAGGAT
    GAGGCCCGGT TTGATGAGAC GTTCCGATGG CCGATTGCCA GTAACATCGA TGGGAATGAA
    ATGCTGGAGA TCCAAGTGTT TAACTACAGC AAAGTCTTCA CTAACAGGCT GATCGGGACG
    TTCCGGATGG TTCTGCAGCA GGTGGTGGAG GAGGCCCAGC TGGAAGTGAC AGACACACTC
    ATCGATGACA ATAACTCGGC AATCCGGACG AGCGTCAGCA TTGAGCTCCG GTACCAGACC
    ATGGACGGTT CCGTCGGTGC CTGGAACGAT GGAGAATTTA TGGACGGTAC CAACCTGCAG
    AGCGAGGCAG TTGGGAATTT CCACTTTGAG ACGGAAAGTC TGCTCTCCGG CCAGAGCCAC
    AATTCTGCCG TGTCCCCCGG GAGATCACAG AGCTTGCACT CAGGGGAGAG AGCCTTTAAA
    AGAGTTGGCA AAGGGGTATT TTCAGCCATG AAGCTTGGGA AGACGCGTCC GCCCAAGGAC
    GAGAGCAGAA AGCAAGATGA ACCTGCTGTG CTGGAGACAG AAGACTTGGA CAGGAAAGCA
    ATCAGATTGG CTGGAGGTCT GGACCCCGAC ACCATCTCTC TGGCCTCCGT TACAGCCGTC
    ACCACCAATG TGTCCAATAA AAGGTCCAAA CCTGATATCA AAATGGAGCC AAGTGCTGGG
    CGGCCAATGG ATTACCAAGT GAGCATCACC GTCATAGAGG CCCGACAGCT GGTGGGACTG
    AACATGGACC CGGTGGTGTG TGTGGAGGTG GGGGACGAGA AGAAGTACAC GTCCATGAAG
    GAATCCACTA ACTGCCCCTA CTACAATGAG TACTTTGTCT TCGATTTCCA CGTCCCCCCC
    GACGTGATGT TTGACAAGAT AATCAAGCTG TCGGTGATCC ATTCCAAGAA TCTGCTCCGG
    AGCGGAACCT TAGTGGGCTC CTTTAAGCTG GATGTTGGCA CTGTGTACAC GCAGCCCGAG
    CACCAGTTCT ACCACAAGTG GGCCCTCCTC TCCGACCCCG ACGACATCAC GGCCGGGCTC
    AAAGGCTACC TGAAGTGTGA CATCGCCGTG GTGGGAAAGG GGGACAACAT CAAGACCCCG
    CACAAAGCGA ACGAGACCGA TGAGGATGAT ATCGAGGGGA ACCTGCTCCT CCCGGACGGA
    GTTCCCGCGG AACGTCAGTG GGCTCGATTC TACGTCAAGA TCTACCGAGC CGAAGGCCTG
    CCCAGGATGA ACACCAGCAT CATGGCCAAC GTCAAGAAAG CTCTCATTGG AGAAAACAAG
    GACTTGGTGG ACCCCTATGT GCAAGTTGCC TTTGCAGGGC AGAAGGGAAA GACCTCGGTG
    CAGAAGAGCA GCTACGAGCC CATGTGGAAC GAGCAGATCA TTTTCACCGA GATGTTCCCC
    CCCCTGTGCA AGAGGATGAA AGTGCAGATC AGAGATTCCG ACAAGGTCAA CGATGTCGCC
    ATTGGGACCC ACTTCATTGA CCTGAGGAAA ATTGCCAACG AGGGGGACAA GGGATTCCTC
    CCTACTTTCG GCCCAGCCTG GGTGAATATG TATGGCTCCA CCCGTAACTA CACCCTCATG
    GATGAGCACC AGGATCTGAA TGAGGGGCTC GGAGAAGGCG TCTCTTTCCG CGCAAGGCTG
    CTGCTGAGTC TCGCTGTTGA GATACTGGAT ACAACCAACC CAGAGCTCAC CAGTTCCACA
    GAGGTTCAAA TTGAAGGAGC TCAACCAGTG GCAGAAACCT GTACCGGTAA AATGGAAGAG
    TTCTTCCTGT TTGGAGCATT CCTGGAATCC ACCATGATTG ATCGGAAAAT TGGGGACAAG
    CCAATTAACT TTGAGGTCAC CATAGGTAAC TATGGCAACC AAATAGATGG AATTTCCAAG
    CCGAGTATGA GGAAGAGGAA GGAAGGAGGT GATGGAGTTG AGGAGGAGAC GGAGCTCTTG
    CAGAATTCCA GTGATGATGA GGCTGATGAA GATGGAGAAA TGTTATCAAT CTCTTCGTCC
    CCTGCAATGA AGCCACTCAT AACCGATAGG AACTACTTCC ACCTCCCGTT CTTTGATAAG
    AAGCCCTGTA CCTACATTAA GAGTTGGTGG CAGGACCAGC GGAGGCGGCT CTACAATGCC
    AACATTATGG ACAAGATCGC AGATAAGCTG GAGGAAGGCT TGAACGATGT TCATGAGATG
    ATAAAGACTG AGAAGCCTTA CCCCGAGCGG CGGCTGCGAG GAGTCCTGGA GGAGCTGAGT
    AGCAGCTGTT TTCGATTTGT TACCTTGGCC AACAAGGACC AGAACCAGAA TGGGCAGACG
    AAACTGGACA GGGAGAGGCT GAAGTCCTGC ATGAGGGAGC TGGAGAACAT GGGGACACAA
    GCCAAGACCA TGAGGTCCCA AGTGAAGAAG AACACGGTTA AAGACAAACT GAAACTGGTG
    CAGAATTTCC TACAGAAGCT CCGATTCCTG GCAGATGAGC CCCAGCACAG CATTCCTGAC
    ATCTTCATCT GGATGATGTC CAACAACAAG CGCATCGCCT ACGCTCGAAT CCCTTCCAAA
    GATATCCTGT ACTCCATTGT GGATGAGGAG ACGGGAAAGG ACTGTGGCAA AGTCAAGTGT
    GTCTTCCTTA AGCTGCCGGG CAAGAGGGGC TTTGGGCCTG CAGGCTGGAC GGTACAAGCC
    AAGATGGAGG TCTACATGTG GTTGGGCCTT AGCAAGCAGC GCAGGGATTT CTTGTGTGGT
    CTTCCATGTG GATTTGAGGA GATAAAAGCA GTGAAAGGGC CTGGTCTGCA GTCCTTCCCA
    CCCATCAGCC TTAGCTACAC CAAAAAACAA GTGTTCCAGT TACGAGCCCA CATGTACCAA
    GCCCGGAGTC TCTTTGCCGC TGACAGCAGT GGACTTTCCG ACCCCTTTGC CCGCGTCTTC
    TTCAACACTC AGAGCCAATG TACAGAGGTG CTGAATGAGA CCCTTTGTCC CACTTGGGAC
    CAGCTGTTGG TGTTTGACAA TATCGAACTC TACGGAGAAG CTCACGAGAT GAGAGACGAC
    CCCCCAATCA TTGTCATTGA GATTTATGAT CAAGACACAG TAGGGAAGGC CGATTTCATG
    GGGAGAACAT TTGCCAAACC TGTCGTGAAA ATGTCAGATG AGCGGTACTG CCCTCCCCGC
    TTTCCGCCAC AGCTGGAATA CTACCAGATC TACCGTGGGA ACTCAACCGC AGGGGACCTC
    TTGGGAGCTT TCGAACTTCT CCAGATCGGA CCAGCGGGCA AGTCTGACCT CCCACAGATT
    GAAGGACCAA CAGACATAGA GAGAGGACCC ATCCTTCCAG TTCCTCTTGG AATCAGACCA
    GTGCTCAGCA AATACAGAGT GGAGGTCCTT TTCTGGGGGC TGAGAGACCT GAAGAGAGTA
    AATCTAGCCC AGGTTGATAG ACCAAGGGTG GATATTGAGT GTGCCGGGAG GGGGGTGCAG
    TCTGCCCTCA TCCAGAACTA CAAGAAGAAT CCAAATTTTA GCACGTTGGT CAAATGGTTT
    GAAGTGGATC TCCCCGAGAA CGAGTTACTT CACCCGCCAT TGAATATCCG AGTAGTAGAC
    TGCCGAGCCT TTGGCAGATA CACTCTGGTT GGTTCCCATG CTGTCAGCTC CCTACGCAGG
    TTCATTTACC GGCCTCCGGA TAAGAACTCC CAGCAGTGGA CCACAGCAGC TAAACTCATG
    AATGGCTACT TGGCAATGAC CAATGGAAGG CCCCGCTCTC GCACCACAGG TGAAATAGTT
    ATCAACATGG AGCCTGAAGC GCCTGTCAAG AAGATGGAGA CAATGGTGAA ACTGGAAGCC
    AATTCCGATG CGGTGGTTAA AGTAGACGTG GGCGAGGAAG AAAAAGACAA GAAGAAGAAG
    AAGAAGAAAG GAGTAGAGGA GATAGAGGAA GAGGAGCCAG ATGAGAGCAT GTTGGACTGG
    TGGTCCAAAT ACTTTGCTTC CATTGAAACC ATGAAGGAGC AACTGCGCCA ACAGGAAGCG
    GCAGCCGCCG AAGCCGAAGA GAAGGAAGAG ATGGAGATCG CAGAGGAGAT CAAAACTGAT
    GACTCTCCCA TGAAAGGCAA CAAGGGCCCC ACGAAGAACA AGGAGAAGGT CAAAGCCCCA
    AAAGAGGAGA AGAAGAACAA GAAGCAGCAA CAGCAGCAGC AGCACCAAGC CGATCAGGCC
    GAGAAGAAGA ACAAGCAAAA GATCGATGAG TTAAAGGTGT ACAACAAGGA GTTGGAGACC
    GAGTATGACA ACTTTGAGGA CTGGCTCCAT ACATTTAACT TGTTACGAGG GAAGATTGGA
    GATGATGACG ATACTGTAAG CGAAGAAGAG CGAATTGTTG GGAGGTTTAA GGGTTCCATG
    TGTGTGTACA AAGTTCCTTT GCCAGAAGAC GTCACAAAGG AAGCCGGGTA TGACCCAAAC
    TTCGGCATGT TCCAAGGAAT CCCTAACAAC GACCCAATGA ATGTGCTGGT CAGAGTTTAT
    GTCGTGAGGG CCACCGATCT TCATCCAGCT GATATCAATG GTAAAGCGGA TCCATATGTT
    GTCATAAAAC TAGGAAAAAC TGATATAAAA GATAAGGAAA ACTACATCTC CAAGCAGCTA
    AACCCTGTCT TCGGAAAGTC CTTTGATATT GAGGCCACTT TTCCGATGGA GTCCATGCTT
    ACAGTTGCGG TTTATGACTG GGATCTGGTG GGGACGGATG ATCTGATCGG TGAAACCAAA
    ATCGACCTGG AAAACCGTTA CTACAGCAAG CACAGGGCGA CTTGTGGTAT CTCAAAGACA
    TATGCCATTC ACGGGTACAA CTTGTGGAGG GACCCAGTGA AGCCTACACA AATCTTATCC
    AAACTTTGCA AAGAAGGGAA GATCGATGGA CCTCATTATG GCCCTGGTGG AAGAGTGAAA
    GTCACGAATC GGGTATTCAC TGGTCCCACA GAAATTGATG ATGAAAATGG TCAGAAGAAA
    CAAACGGATG AGCACCTTGC ACTCTCGGTC CTTCAGCACT GGGAGGACAT TCCTCGGGTG
    GGATGTAAGC TGGTCCCAGA GCATGTAGAA ACGAGGCCTT TGTTAAACCC AGACAAACCT
    GGAATTGAGC AGGGCCGCAT AGAAATGTGG GTAGACATGT TCCCCATGGA CATGCCTGCG
    CCAGGTCCTG CCATTGATAT CTCTCCAAGG AAGCCAAAAA GATACGAGCT TAGAGTGATA
    ATATGGAATA CAGATGAGGT AATCCTGGAA GATGATGATT ACTTCACAGG AGAAAAATCT
    AGTGACATAT TTGTCAGGGG GTGGCTGAAG GGACAGCAGG AAGACAAACA AGACACGGAT
    GTCCACTATC ATTCCCTTAC TGGGGAGGGG AACTTCAACT GGAGGTTCTT GTTCCCTTTT
    GATTACCTTA TGGCAGAGGA GAAGATTGTC ATCTCCAAGA AAGAGTCCAT GTTCTCTTGG
    GATGAGACCG AGTACAAGAT CCCGGCTCGT CTCACTCTCC AGGTCTGGGA TGCCGACCAC
    TTCTCAGCAG ATGACTTTCT CGGAGCCATA GAATTAGACT TGAATCGCTT TCCCCGAGGG
    GCAAAAACAG CGAAACAGTG TTCGATAGAA ATGGCGGAAC CCGACGTCGA TCTGCCAATG
    GTCTCAATCT TCAAACAGAA GAGGGTGAAG GGATGGTGGC CTTTCGTTGC CAGGAATGAA
    AACGATGAGA TGGAGCTGAC TGGTAAAGTG GAGGCGGAGC TTCACCTTCT CTCAGCAGAG
    GAGGCAGAGA AGACCCCGGC AGGGCTTGGG CGCAATGAAC CAGATCCTCT TGAAAAACCA
    AATCGACCCG ACACCTCGTT CATCTGGTTC CTCAACCCAC TTAAGTCCAT CAAGTATCTC
    ATCTGTAATC GCTACAAGTG GCTCATCATT AAGATTGTGC TGGCGTTGCT GCTTTTGGCC
    ATGGTGGCCC TCTTCCTCTA CAGCATGCCC GGTTACATGG TGAAGAAGCT TCTGGGTGCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012826776.1
    CDS71..6133
    Translation

    Target ORF information:

    RefSeq Version XM_012971322.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis otoferlin (otof), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012971322.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCCCTAA TAGTGCATCT CAGGACTGTC ACCGACCTGC GGGGGAAAGG GGACCGGATC 
    GCCAAAGTGA CGTTCAGAGG ACTGTCGTTC TACACGCGGG TGTTGGAGAA CTGTGAGGAT
    GAGGCCCGGT TTGATGAGAC GTTCCGATGG CCGATTGCCA GTAACATCGA TGGGAATGAA
    ATGCTGGAGA TCCAAGTGTT TAACTACAGC AAAGTCTTCA CTAACAGGCT GATCGGGACG
    TTCCGGATGG TTCTGCAGCA GGTGGTGGAG GAGGCCCAGC TGGAAGTGAC AGACACACTC
    ATCGATGACA ATAACTCGGC AATCCGGACG AGCGTCAGCA TTGAGCTCCG GTACCAGACC
    ATGGACGGTT CCGTCGGTGC CTGGAACGAT GGAGAATTTA TGGACGGTAC CAACCTGCAG
    AGCGAGGCAG TTGGGAATTT CCACTTTGAG ACGGAAAGTC TGCTCTCCGG CCAGAGCCAC
    AATTCTGCCG TGTCCCCCGG GAGATCACAG AGCTTGCACT CAGGGGAGAG AGCCTTTAAA
    AGAGTTGGCA AAGGGGTATT TTCAGCCATG AAGCTTGGGA AGACGCGTCC GCCCAAGGAC
    GAGAGCAGAA AGCAAGATGA ACCTGCTGTG CTGGAGACAG AAGACTTGGA CAGGAAAGCA
    ATCAGATTGG CTGGAGGTCT GGACCCCGAC ACCATCTCTC TGGCCTCCGT TACAGCCGTC
    ACCACCAATG TGTCCAATAA AAGGTCCAAA CCTGATATCA AAATGGAGCC AAGTGCTGGG
    CGGCCAATGG ATTACCAAGT GAGCATCACC GTCATAGAGG CCCGACAGCT GGTGGGACTG
    AACATGGACC CGGTGGTGTG TGTGGAGGTG GGGGACGAGA AGAAGTACAC GTCCATGAAG
    GAATCCACTA ACTGCCCCTA CTACAATGAG TACTTTGTCT TCGATTTCCA CGTCCCCCCC
    GACGTGATGT TTGACAAGAT AATCAAGCTG TCGGTGATCC ATTCCAAGAA TCTGCTCCGG
    AGCGGAACCT TAGTGGGCTC CTTTAAGCTG GATGTTGGCA CTGTGTACAC GCAGCCCGAG
    CACCAGTTCT ACCACAAGTG GGCCCTCCTC TCCGACCCCG ACGACATCAC GGCCGGGCTC
    AAAGGCTACC TGAAGTGTGA CATCGCCGTG GTGGGAAAGG GGGACAACAT CAAGACCCCG
    CACAAAGCGA ACGAGACCGA TGAGGATGAT ATCGAGGGGA ACCTGCTCCT CCCGGACGGA
    GTTCCCGCGG AACGTCAGTG GGCTCGATTC TACGTCAAGA TCTACCGAGC CGAAGGCCTG
    CCCAGGATGA ACACCAGCAT CATGGCCAAC GTCAAGAAAG CTCTCATTGG AGAAAACAAG
    GACTTGGTGG ACCCCTATGT GCAAGTTGCC TTTGCAGGGC AGAAGGGAAA GACCTCGGTG
    CAGAAGAGCA GCTACGAGCC CATGTGGAAC GAGCAGATCA TTTTCACCGA GATGTTCCCC
    CCCCTGTGCA AGAGGATGAA AGTGCAGATC AGAGATTCCG ACAAGGTCAA CGATGTCGCC
    ATTGGGACCC ACTTCATTGA CCTGAGGAAA ATTGCCAACG AGGGGGACAA GGGATTCCTC
    CCTACTTTCG GCCCAGCCTG GGTGAATATG TATGGCTCCA CCCGTAACTA CACCCTCATG
    GATGAGCACC AGGATCTGAA TGAGGGGCTC GGAGAAGGCG TCTCTTTCCG CGCAAGGCTG
    CTGCTGAGTC TCGCTGTTGA GATACTGGAT ACAACCAACC CAGAGCTCAC CAGTTCCACA
    GAGGTTCAAA TTGAAGGAGC TCAACCAGTG GCAGAAACCT GTACCGGTAA AATGGAAGAG
    TTCTTCCTGT TTGGAGCATT CCTGGAATCC ACCATGATTG ATCGGAAAAT TGGGGACAAG
    CCAATTAACT TTGAGGTCAC CATAGGTAAC TATGGCAACC AAATAGATGG AATTTCCAAG
    CCGAGTATGA GGAAGAGGAA GGAAGGAGGT GATGGAGTTG AGGAGGAGAC GGAGCTCTTG
    CAGAATTCCA GTGATGATGA GGCTGATGAA GATGGAGAAA TGTTATCAAT CTCTTCGTCC
    CCTGCAATGA AGCCACTCAT AACCGATAGG AACTACTTCC ACCTCCCGTT CTTTGATAAG
    AAGCCCTGTA CCTACATTAA GAGTTGGTGG CAGGACCAGC GGAGGCGGCT CTACAATGCC
    AACATTATGG ACAAGATCGC AGATAAGCTG GAGGAAGGCT TGAACGATGT TCATGAGATG
    ATAAAGACTG AGAAGCCTTA CCCCGAGCGG CGGCTGCGAG GAGTCCTGGA GGAGCTGAGT
    AGCAGCTGTT TTCGATTTGT TACCTTGGCC AACAAGGACC AGAACCAGAA TGGGCAGACG
    AAACTGGACA GGGAGAGGCT GAAGTCCTGC ATGAGGGAGC TGGAGAACAT GGGGACACAA
    GCCAAGACCA TGAGGTCCCA AGTGAAGAAG AACACGGTTA AAGACAAACT GAAACTGGTG
    CAGAATTTCC TACAGAAGCT CCGATTCCTG GCAGATGAGC CCCAGCACAG CATTCCTGAC
    ATCTTCATCT GGATGATGTC CAACAACAAG CGCATCGCCT ACGCTCGAAT CCCTTCCAAA
    GATATCCTGT ACTCCATTGT GGATGAGGAG ACGGGAAAGG ACTGTGGCAA AGTCAAGTGT
    GTCTTCCTTA AGCTGCCGGG CAAGAGGGGC TTTGGGCCTG CAGGCTGGAC GGTACAAGCC
    AAGATGGAGG TCTACATGTG GTTGGGCCTT AGCAAGCAGC GCAGGGATTT CTTGTGTGGT
    CTTCCATGTG GATTTGAGGA GATAAAAGCA GTGAAAGGGC CTGGTCTGCA GTCCTTCCCA
    CCCATCAGCC TTAGCTACAC CAAAAAACAA GTGTTCCAGT TACGAGCCCA CATGTACCAA
    GCCCGGAGTC TCTTTGCCGC TGACAGCAGT GGACTTTCCG ACCCCTTTGC CCGCGTCTTC
    TTCAACACTC AGAGCCAATG TACAGAGGTG CTGAATGAGA CCCTTTGTCC CACTTGGGAC
    CAGCTGTTGG TGTTTGACAA TATCGAACTC TACGGAGAAG CTCACGAGAT GAGAGACGAC
    CCCCCAATCA TTGTCATTGA GATTTATGAT CAAGACACAG TAGGGAAGGC CGATTTCATG
    GGGAGAACAT TTGCCAAACC TGTCGTGAAA ATGTCAGATG AGCGGTACTG CCCTCCCCGC
    TTTCCGCCAC AGCTGGAATA CTACCAGATC TACCGTGGGA ACTCAACCGC AGGGGACCTC
    TTGGGAGCTT TCGAACTTCT CCAGATCGGA CCAGCGGGCA AGTCTGACCT CCCACAGATT
    GAAGGACCAA CAGACATAGA GAGAGGACCC ATCCTTCCAG TTCCTCTTGG AATCAGACCA
    GTGCTCAGCA AATACAGAGT GGAGGTCCTT TTCTGGGGGC TGAGAGACCT GAAGAGAGTA
    AATCTAGCCC AGGTTGATAG ACCAAGGGTG GATATTGAGT GTGCCGGGAG GGGGGTGCAG
    TCTGCCCTCA TCCAGAACTA CAAGAAGAAT CCAAATTTTA GCACGTTGGT CAAATGGTTT
    GAAGTGGATC TCCCCGAGAA CGAGTTACTT CACCCGCCAT TGAATATCCG AGTAGTAGAC
    TGCCGAGCCT TTGGCAGATA CACTCTGGTT GGTTCCCATG CTGTCAGCTC CCTACGCAGG
    TTCATTTACC GGCCTCCGGA TAAGAACTCC CAGCAGTGGA CCACAGCAGC TAAACTCATG
    AATGGCTACT TGGCAATGAC CAATGGAAGG CCCCGCTCTC GCACCACAGG TGAAATAGTT
    ATCAACATGG AGCCTGAAGC GCCTGTCAAG AAGATGGAGA CAATGGTGAA ACTGGAAGCC
    AATTCCGATG CGGTGGTTAA AGTAGACGTG GGCGAGGAAG AAAAAGACAA GAAGAAGAAG
    AAGAAGAAAG GAGTAGAGGA GATAGAGGAA GAGGAGCCAG ATGAGAGCAT GTTGGACTGG
    TGGTCCAAAT ACTTTGCTTC CATTGAAACC ATGAAGGAGC AACTGCGCCA ACAGGAAGCG
    GCAGCCGCCG AAGCCGAAGA GAAGGAAGAG ATGGAGATCG CAGAGGAGAT CAAAACTGAT
    GACTCTCCCA TGAAAGGCAA CAAGGGCCCC ACGAAGAACA AGGAGAAGGT CAAAGCCCCA
    AAAGAGGAGA AGAAGAACAA GAAGCAGCAA CAGCAGCAGC AGCACCAAGC CGATCAGGCC
    GAGAAGAAGA ACAAGCAAAA GATCGATGAG TTAAAGGTGT ACAACAAGGA GTTGGAGACC
    GAGTATGACA ACTTTGAGGA CTGGCTCCAT ACATTTAACT TGTTACGAGG GAAGATTGGA
    GATGATGACG ATACTGTAAG CGAAGAAGAG CGAATTGTTG GGAGGTTTAA GGGTTCCATG
    TGTGTGTACA AAGTTCCTTT GCCAGAAGAC GTCACAAAGG AAGCCGGGTA TGACCCAAAC
    TTCGGCATGT TCCAAGGAAT CCCTAACAAC GACCCAATGA ATGTGCTGGT CAGAGTTTAT
    GTCGTGAGGG CCACCGATCT TCATCCAGCT GATATCAATG GTAAAGCGGA TCCATATGTT
    GTCATAAAAC TAGGAAAAAC TGATATAAAA GATAAGGAAA ACTACATCTC CAAGCAGCTA
    AACCCTGTCT TCGGAAAGTC CTTTGATATT GAGGCCACTT TTCCGATGGA GTCCATGCTT
    ACAGTTGCGG TTTATGACTG GGATCTGGTG GGGACGGATG ATCTGATCGG TGAAACCAAA
    ATCGACCTGG AAAACCGTTA CTACAGCAAG CACAGGGCGA CTTGTGGTAT CTCAAAGACA
    TATGCCATTC ACGGGTACAA CTTGTGGAGG GACCCAGTGA AGCCTACACA AATCTTATCC
    AAACTTTGCA AAGAAGGGAA GATCGATGGA CCTCATTATG GCCCTGGTGG AAGAGTGAAA
    GTCACGAATC GGGTATTCAC TGGTCCCACA GAAATTGATG ATGAAAATGG TCAGAAGAAA
    CAAACGGATG AGCACCTTGC ACTCTCGGTC CTTCAGCACT GGGAGGACAT TCCTCGGGTG
    GGATGTAAGC TGGTCCCAGA GCATGTAGAA ACGAGGCCTT TGTTAAACCC AGACAAACCT
    GGAATTGAGC AGGGCCGCAT AGAAATGTGG GTAGACATGT TCCCCATGGA CATGCCTGCG
    CCAGGTCCTG CCATTGATAT CTCTCCAAGG AAGCCAAAAA GATACGAGCT TAGAGTGATA
    ATATGGAATA CAGATGAGGT AATCCTGGAA GATGATGATT ACTTCACAGG AGAAAAATCT
    AGTGACATAT TTGTCAGGGG GTGGCTGAAG GGACAGCAGG AAGACAAACA AGACACGGAT
    GTCCACTATC ATTCCCTTAC TGGGGAGGGG AACTTCAACT GGAGGTTCTT GTTCCCTTTT
    GATTACCTTA TGGCAGAGGA GAAGATTGTC ATCTCCAAGA AAGAGTCCAT GTTCTCTTGG
    GATGAGACCG AGTACAAGAT CCCGGCTCGT CTCACTCTCC AGGTCTGGGA TGCCGACCAC
    TTCTCAGCAG ATGACTTTCT CGGAGCCATA GAATTAGACT TGAATCGCTT TCCCCGAGGG
    GCAAAAACAG CGAAACAGTG TTCGATAGAA ATGGCGGAAC CCGACGTCGA TCTGCCAATG
    GTCTCAATCT TCAAACAGAA GAGGGTGAAG GGATGGTGGC CTTTCGTTGC CAGGAATGAA
    AACGATGAGA TGGAGCTGAC TGGTAAAGTG GAGGCGGAGC TTCACCTTCT CTCAGCAGAG
    GAGGCAGAGA AGACCCCGGC AGGGCTTGGG CGCAATGAAC CAGATCCTCT TGAAAAACCA
    AATCGACCCG ACACCTCGTT CATCTGGTTC CTCAACCCAC TTAAGTCCAT CAAGTATCTC
    ATCTGTAATC GCTACAAGTG GCTCATCATT AAGATTGTGC TGGCGTTGCT GCTTTTGGCC
    ATGGTGGCCC TCTTCCTCTA CAGCATGCCC GGTTACATGG TGAAGAAGCT TCTGGGTGCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
    Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
    Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,


    Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
    Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
    Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P