Dio2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dio2
Entrez Gene ID	65162
Full Name	iodothyronine deiodinase 2
Synonyms	5DII,DIOII
General protein information	Preferred Names iodothyronine deiodinase 2 Names type II iodothyronine deiodinase deiodinase, iodothyronine, type 2 deiodinase, iodothyronine, type II type 2 DI type-II 5'-deiodinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q31
Summary	The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination. This gene is expressed in a few tissues in rat, including brain, pituitary and brown adipose tissue. It is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues. Studies in rat suggest that this gene may play an important role in the regulation of lipid metabolism and thermogenesis, and in osteoarthritis pathogenesis by enhancing the chondrocyte hypertrophy and inflammatory response. This protein is a selenoprotein containing the rare selenocysteine (Sec) amino acid at its active site, and may contain additional Sec residues. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, May 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031720.5	NP_113908.4	type II iodothyronine deiodinase
NM_031720.4	NP_113908.3	type II iodothyronine deiodinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIO2	NP_001007024.1
Bos taurus (cattle)	DIO2	NP_001010992.1
Danio rerio (zebrafish)	dio2	NP_997954.2
Xenopus tropicalis (tropical clawed frog)	dio2	NP_001184161.2
Pan troglodytes (chimpanzee)	DIO2	NP_001116109.1
Canis lupus familiaris (dog)	DIO2	NP_001116117.1
	DIO2	NP_001152766.1
Mus musculus (house mouse)	Dio2	NP_034180.1
Rattus norvegicus (Norway rat)	Dio2	NP_113908.3
Gallus gallus (chicken)	DIO2	NP_989445.2

Gene Ontology
Bibliography

Related articles in PubMed

Effect of glucocorticoids on the activity, expression and proximal promoter of type II deiodinase in rat brown adipocytes.
Martinez-deMena R, Calvo RM, Garcia L, Obregon MJ
Molecular and cellular endocrinology42858-67(2016 06)

Antidepressant behavior in thyroidectomized Wistar rats is induced by hippocampal hypothyroidism.
da Concei??o RR, Laureano-Melo R, Oliveira KC, de Carvalho Melo MC, Kasamatsu TS, de Barros Maciel RM, de Souza JS, Giannocco G
Physiology & behavior157158-64(2016 Apr)

Differences in hypothalamic type 2 deiodinase ubiquitination explain localized sensitivity to thyroxine.
Werneck de Castro JP, Fonseca TL, Ueta CB, McAninch EA, Abdalla S, Wittmann G, Lechan RM, Gereben B, Bianco AC
The Journal of clinical investigation125(2)769-81(2015 Feb)

Type 2 iodothyronine deiodinase is upregulated in rat slow- and fast-twitch skeletal muscle during cold exposure.
Louzada RA, Santos MC, Cavalcanti-de-Albuquerque JP, Rangel IF, Ferreira AC, Galina A, Werneck-de-Castro JP, Carvalho DP
American journal of physiology. Endocrinology and metabolism307(11)E1020-9(2014 Dec)

NF?B signaling is essential for the lipopolysaccharide-induced increase of type 2 deiodinase in tanycytes.
de Vries EM, Kwakkel J, Eggels L, Kalsbeek A, Barrett P, Fliers E, Boelen A
Endocrinology155(5)2000-8(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Glucocorticoids inhibited the adrenergic stimulation of Dio2 at the transcriptional level in brown adipocytes.
Title: Effect of glucocorticoids on the activity, expression and proximal promoter of type II deiodinase in rat brown adipocytes.

This study demonstrated that reduction of the Dio2 mRNA expression in hippocampus of thyroidectomized Wistar rats.
Title: Antidepressant behavior in thyroidectomized Wistar rats is induced by hippocampal hypothyroidism.

These studies reveal that tissue-specific differences in D2 ubiquitination are an inherent property of the TRH/TSH feedback mechanism
Title: Differences in hypothalamic type 2 deiodinase ubiquitination explain localized sensitivity to thyroxine.

cold exposure is accompanied by concerted changes in the metabolism of BAT and oxidative and glycolytic skeletal muscles that are paralleled by type 2 deiodinase activation
Title: Type 2 iodothyronine deiodinase is upregulated in rat slow- and fast-twitch skeletal muscle during cold exposure.

Data suggest that type 2 deiodinase is induced in inflammation of tanycytes (as in inflammation of periventricular area, arcuate nucleus, and median eminence of hypothalamus); this enzyme induction appears to involve NFkappaB signaling.
Title: NF?B signaling is essential for the lipopolysaccharide-induced increase of type 2 deiodinase in tanycytes.

The following Dio2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dio2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12185	NM_031720.4 Latest version!	Rattus norvegicus iodothyronine deiodinase 2 (Dio2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa63310	NM_031720.5 Latest version!	Rattus norvegicus iodothyronine deiodinase 2 (Dio2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dio2 ( NM_031720.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113908.3 Rattus norvegicus iodothyronine deiodinase 2 (Dio2), mRNA.

Price & Availability↑

CloneID	ORa12185
Clone ID Related Accession (Same CDS sequence)	NM_031720.4
Accession Version	NM_031720.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-09
Organism	Rattus norvegicus(Norway rat)
Product	type II iodothyronine deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC118957.13. On May 11, 2016 this sequence version replaced NM_031720.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U53505.1, BF565729.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 8755651 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGACTCC TCAGCGTAGA CTTGCTGATC ACTCTTCAGA TTCTGCCAGT CTTTTTCTCC 
AACTGCCTCT TCCTGGCGCT CTATGACTCG GTCATTCTGC TCAAGCACGT GGCGCTGCTT 
CTCAGCCGCT CCAAGTCCAC TCGCGGAGAG TGGAGGCGCA TGCTGACCTC AGAAGGACTA 
CGCTGTGTCT GGAACAGCTT TCTCCTAGAC GCCTACAAAC AGGTTAAATT GGGTGAAGAT 
GCTCCCAATT CCAGTGTGGT GCACGTGTCC AATCCTGAAG CAGGTAACAA TTGTGCCTCA 
GAGAAGACGG CGGATGGGGC TGAATGCCAC CTTCTTGACT TTGCCAGTGC AGAGCGCCCA 
CTGGTGGTCA ACTTTGGTTC AGCGACCTGA CCACCTTTTA CTAGGCAACT GCCAGCCTTC 
CGCCAGTTGG TGGAAGAGTT CTCCTCGGTG GCTGACTTCC TGTTGGTATA CATTGATGAG 
GCTCACCCTT CAGATGGCTG GGCAGTGCCT GGGGACTCCT CTATGTCTTT TGAGGTTAAG 
AAGCACCGGA ACCAAGAGGA CCGATGTGCT GCAGCCCACC AGCTCCTGGA GCGTTTCTCC 
TTGCCGCCCC AGTGTCAAGT TGTGGCTGAC CGCATGGACA ATAATGCCAA CGTAGCTTAT 
GGGGTAGCCT TTGAACGTGT GTGCATCGTG CAGAGACGGA AAATTGCTTA CTTAGGAGGG 
AAGGGCCCCT TCAGCTATAA CCTGCAAGAA GTCCGAAGTT GGCTGGAGAA GAATTTCAGC 
AAGAGATGAA TTCTAGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113908.3
CDS	564..1364
Translation	MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHVALLLSRSKSTRGEWRRMLTSEGLRCVWNSFLLDAYKQVKLGEDAPNSSVVHVSNPEAGNNCASEKTADGAECHLLDFASAERPLVVNFGSATPPFTRQLPAFRQLVEEFSSVADFLLVYIDEAHPSDGWAVPGDSSMSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADRMDNNANVAYGVAFERVCIVQRRKIAYLGGKGPFSYNLQEVRSWLEKNFSKRILD

Target ORF information:

RefSeq Version	NM_031720.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus iodothyronine deiodinase 2 (Dio2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031720.4

ORF Insert Sequence:

ATGGGACTCC TCAGCGTAGA CTTGCTGATC ACTCTTCAGA TTCTGCCAGT CTTTTTCTCC 
AACTGCCTCT TCCTGGCGCT CTATGACTCG GTCATTCTGC TCAAGCACGT GGCGCTGCTT 
CTCAGCCGCT CCAAGTCCAC TCGCGGAGAG TGGAGGCGCA TGCTGACCTC AGAAGGACTA 
CGCTGTGTCT GGAACAGCTT TCTCCTAGAC GCCTACAAAC AGGTTAAATT GGGTGAAGAT 
GCTCCCAATT CCAGTGTGGT GCACGTGTCC AATCCTGAAG CAGGTAACAA TTGTGCCTCA 
GAGAAGACGG CGGATGGGGC TGAATGCCAC CTTCTTGACT TTGCCAGTGC AGAGCGCCCA 
CTGGTGGTCA ACTTTGGTTC AGCGACCTGA CCACCTTTTA CTAGGCAACT GCCAGCCTTC 
CGCCAGTTGG TGGAAGAGTT CTCCTCGGTG GCTGACTTCC TGTTGGTATA CATTGATGAG 
GCTCACCCTT CAGATGGCTG GGCAGTGCCT GGGGACTCCT CTATGTCTTT TGAGGTTAAG 
AAGCACCGGA ACCAAGAGGA CCGATGTGCT GCAGCCCACC AGCTCCTGGA GCGTTTCTCC 
TTGCCGCCCC AGTGTCAAGT TGTGGCTGAC CGCATGGACA ATAATGCCAA CGTAGCTTAT 
GGGGTAGCCT TTGAACGTGT GTGCATCGTG CAGAGACGGA AAATTGCTTA CTTAGGAGGG 
AAGGGCCCCT TCAGCTATAA CCTGCAAGAA GTCCGAAGTT GGCTGGAGAA GAATTTCAGC 
AAGAGATGAA TTCTAGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dio2 ( NM_031720.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113908.4 Rattus norvegicus iodothyronine deiodinase 2 (Dio2), mRNA.

Price & Availability↑

CloneID	ORa63310
Clone ID Related Accession (Same CDS sequence)	NM_031720.5
Accession Version	NM_031720.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Rattus norvegicus(Norway rat)
Product	type II iodothyronine deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC118957.13. On Oct 9, 2018 this sequence version replaced NM_031720.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U53505.1, BF565729.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 8755651 ##RefSeq-Attributes-END##

ATGGGACTCC TCAGCGTAGA CTTGCTGATC ACTCTTCAGA TTCTGCCAGT CTTTTTCTCC 
AACTGCCTCT TCCTGGCGCT CTATGACTCG GTCATTCTGC TCAAGCACGT GGCGCTGCTT 
CTCAGCCGCT CCAAGTCCAC TCGCGGAGAG TGGAGGCGCA TGCTGACCTC AGAAGGACTA 
CGCTGTGTCT GGAACAGCTT TCTCCTAGAC GCCTACAAAC AGGTTAAATT GGGTGAAGAT 
GCTCCCAATT CCAGTGTGGT GCACGTGTCC AATCCTGAAG CAGGTAACAA TTGTGCCTCA 
GAGAAGACGG CGGATGGGGC TGAATGCCAC CTTCTTGACT TTGCCAGTGC AGAGCGCCCA 
CTGGTGGTCA ACTTTGGTTC AGCGACCTGA CCACCTTTTA CTAGGCAACT GCCAGCCTTC 
CGCCAGTTGG TGGAAGAGTT CTCCTCGGTG GCTGACTTCC TGTTGGTATA CATTGATGAG 
GCTCACCCTT CAGATGGCTG GGCAGTGCCT GGGGACTCCT CTATGTCTTT TGAGGTTAAG 
AAGCACCGGA ACCAAGAGGA CCGATGTGCT GCAGCCCACC AGCTCCTGGA GCGTTTCTCC 
TTGCCGCCCC AGTGTCAAGT TGTGGCTGAC CGCATGGACA ATAATGCCAA CGTAGCTTAT 
GGGGTAGCCT TTGAACGTGT GTGCATCGTG CAGAGACGGA AAATTGCTTA CTTAGGAGGG 
AAGGGCCCCT TCAGCTATAA CCTGCAAGAA GTCCGAAGTT GGCTGGAGAA GAATTTCAGC 
AAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113908.4
CDS	564..1352
Translation	MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHVALLLSRSKSTRGEWRRMLTSEGLRCVWNSFLLDAYKQVKLGEDAPNSSVVHVSNPEAGNNCASEKTADGAECHLLDFASAERPLVVNFGSAT*PPFTRQLPAFRQLVEEFSSVADFLLVYIDEAHPSDGWAVPGDSSMSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADRMDNNANVAYGVAFERVCIVQRRKIAYLGGKGPFSYNLQEVRSWLEKNFSKR

Target ORF information:

RefSeq Version	NM_031720.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus iodothyronine deiodinase 2 (Dio2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031720.5

ORF Insert Sequence:

ATGGGACTCC TCAGCGTAGA CTTGCTGATC ACTCTTCAGA TTCTGCCAGT CTTTTTCTCC 
AACTGCCTCT TCCTGGCGCT CTATGACTCG GTCATTCTGC TCAAGCACGT GGCGCTGCTT 
CTCAGCCGCT CCAAGTCCAC TCGCGGAGAG TGGAGGCGCA TGCTGACCTC AGAAGGACTA 
CGCTGTGTCT GGAACAGCTT TCTCCTAGAC GCCTACAAAC AGGTTAAATT GGGTGAAGAT 
GCTCCCAATT CCAGTGTGGT GCACGTGTCC AATCCTGAAG CAGGTAACAA TTGTGCCTCA 
GAGAAGACGG CGGATGGGGC TGAATGCCAC CTTCTTGACT TTGCCAGTGC AGAGCGCCCA 
CTGGTGGTCA ACTTTGGTTC AGCGACCTGA CCACCTTTTA CTAGGCAACT GCCAGCCTTC 
CGCCAGTTGG TGGAAGAGTT CTCCTCGGTG GCTGACTTCC TGTTGGTATA CATTGATGAG 
GCTCACCCTT CAGATGGCTG GGCAGTGCCT GGGGACTCCT CTATGTCTTT TGAGGTTAAG 
AAGCACCGGA ACCAAGAGGA CCGATGTGCT GCAGCCCACC AGCTCCTGGA GCGTTTCTCC 
TTGCCGCCCC AGTGTCAAGT TGTGGCTGAC CGCATGGACA ATAATGCCAA CGTAGCTTAT 
GGGGTAGCCT TTGAACGTGT GTGCATCGTG CAGAGACGGA AAATTGCTTA CTTAGGAGGG 
AAGGGCCCCT TCAGCTATAA CCTGCAAGAA GTCCGAAGTT GGCTGGAGAA GAATTTCAGC 
AAGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effect of glucocorticoids on the activity, expression and proximal promoter of type II deiodinase in rat brown adipocytes.
Molecular and cellular endocrinology42858-67(2016 06)
Martinez-deMena R,Calvo RM,Garcia L,Obregon MJ

Antidepressant behavior in thyroidectomized Wistar rats is induced by hippocampal hypothyroidism.
Physiology & behavior157158-64(2016 Apr)
da Concei??o RR,Laureano-Melo R,Oliveira KC,de Carvalho Melo MC,Kasamatsu TS,de Barros Maciel RM,de Souza JS,Giannocco G

Differences in hypothalamic type 2 deiodinase ubiquitination explain localized sensitivity to thyroxine.
The Journal of clinical investigation125(2)769-81(2015 Feb)
Werneck de Castro JP,Fonseca TL,Ueta CB,McAninch EA,Abdalla S,Wittmann G,Lechan RM,Gereben B,Bianco AC

Type 2 iodothyronine deiodinase is upregulated in rat slow- and fast-twitch skeletal muscle during cold exposure.
American journal of physiology. Endocrinology and metabolism307(11)E1020-9(2014 Dec)
Louzada RA,Santos MC,Cavalcanti-de-Albuquerque JP,Rangel IF,Ferreira AC,Galina A,Werneck-de-Castro JP,Carvalho DP

NF?B signaling is essential for the lipopolysaccharide-induced increase of type 2 deiodinase in tanycytes.
Endocrinology155(5)2000-8(2014 May)
de Vries EM,Kwakkel J,Eggels L,Kalsbeek A,Barrett P,Fliers E,Boelen A

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Dio2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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