DIO2 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	DIO2
Entrez Gene ID	373903
Full Name	iodothyronine deiodinase 2
General protein information	Preferred Names iodothyronine deiodinase 2 Names type II iodothyronine deiodinase 5DII DIOII deiodinase, iodothyronine type II type 2 DI type-II 5'-deiodinase
Gene Type	protein-coding
Organism	Gallus gallus(chicken)
Summary	The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination. This gene is expressed in brain, thyroid and lung. It is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues. Unlike in mammals, this gene is also expressed in the liver of chicken, suggesting a role for this enzyme in the generation of plasma T3 in this species. This protein is a selenoprotein containing the rare selenocysteine (Sec) amino acid at its active site, and may contain additional Sec residues. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001324555.2	NP_001311484.1	type II iodothyronine deiodinase isoform b
NM_204114.4	NP_989445.3	type II iodothyronine deiodinase isoform a
NM_001324555.1	NP_001311484.1	type II iodothyronine deiodinase isoform b
NM_204114.3	NP_989445.2	type II iodothyronine deiodinase isoform a

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIO2	NP_001007024.1
Bos taurus (cattle)	DIO2	NP_001010992.1
Danio rerio (zebrafish)	dio2	NP_997954.2
Xenopus tropicalis (tropical clawed frog)	dio2	NP_001184161.2
Pan troglodytes (chimpanzee)	DIO2	NP_001116109.1
Canis lupus familiaris (dog)	DIO2	NP_001116117.1
	DIO2	NP_001152766.1
Mus musculus (house mouse)	Dio2	NP_034180.1
Rattus norvegicus (Norway rat)	Dio2	NP_113908.3
Gallus gallus (chicken)	DIO2	NP_989445.2

Gene Ontology
Bibliography

Related articles in PubMed

Detection of long-term influence of prenatal temperature stimulation on hypothalamic type-II iodothyronine deiodinase in juvenile female broiler chickens using a novel immunohistochemical amplification protocol.
Nassar M, Halle I, Plagemann A, Tzschentke B
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology179120-4(2015 Jan)

Dynamic mRNA distribution pattern of thyroid hormone transporters and deiodinases during early embryonic chicken brain development.
Geysens S, Ferran JL, Van Herck SL, Tylzanowski P, Puelles L, Darras VM
Neuroscience22169-85(2012 Sep)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

Differential expression of iodothyronine deiodinases in chicken tissues during the last week of embryonic development.
Van der Geyten S, Van den Eynde I, Segers IB, K?hn ER, Darras VM
General and comparative endocrinology128(1)65-73(2002 Aug)

The mRNA structure has potent regulatory effects on type 2 iodothyronine deiodinase expression.
Gereben B, Koll?r A, Harney JW, Larsen PR
Molecular endocrinology (Baltimore, Md.)16(7)1667-79(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

significant Dio2 expression was exclusively found in perinatally short-term temperature stimulated hens.
Title: Detection of long-term influence of prenatal temperature stimulation on hypothalamic type-II iodothyronine deiodinase in juvenile female broiler chickens using a novel immunohistochemical amplification protocol.

This study demonistrated that DIO2 expression in the developing pituitary gland and capillaries throughout the brain.
Title: Dynamic mRNA distribution pattern of thyroid hormone transporters and deiodinases during early embryonic chicken brain development.

The following DIO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa00383	NM_204114.3 Latest version!	Gallus gallus iodothyronine deiodinase 2 (DIO2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OGa153300	NM_204114.4 Latest version!	Gallus gallus deiodinase, iodothyronine type II (DIO2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OGa86423	NM_001324555.2 Latest version!	Gallus gallus deiodinase, iodothyronine type II (DIO2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
View Old Accession Versions >>
OGa86423	NM_001324555.1	Gallus gallus iodothyronine deiodinase 2 (DIO2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DIO2 ( NM_204114.3 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989445.2 Gallus gallus iodothyronine deiodinase 2 (DIO2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OGa00383
Clone ID Related Accession (Same CDS sequence)	NM_204114.3
Accession Version	NM_204114.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Gallus gallus(chicken)
Product	type II iodothyronine deiodinase isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN04000015.1. On Feb 23, 2012 this sequence version replaced NM_204114.2. Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF125575.1, BU369644.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201359 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 10318780 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGTCTGT TAAGTGTGGA TTTGCTGATC ACGCTTCAGA TCTTGCCGGT CTTTTTCTCC 
AATTGCCTCT TCCTTGCGCT GTATGACTCT GTGATCCTCC TGAAGCACAT GGTGCTGTTT 
CTGAGCCGCT CCAAGTCTGC GCGCGGTGAG TGGCGGAGGA TGCTGACCTC GGAGGGGCTG 
CGCTGCGTCT GGAACAGCTT CCTCCTGGAC GCCTACAAGC AGGTCAAACT TGGAGGAGAA 
GCTCCGAACT CCAGTGTAAT CCACATAGCC AAGGGCAATG ATGGCAGCAA TAGCAGCTGG 
AAGAGTGTTG GTGGCAAGTG TGGAACCAAG TGCCATCTTC TGGATTTTGC CAACTCTGAG 
CGGCCACTGG TGGTTAATTT TGGCTCAGCT ACCTGACCAC CATTCACAAG CCAGCTGTCT 
GCCTTCAGCA AGCTGGTGGA AGAGTTCTCT GGCGTGGCTG ACTTTCTGTT GGTCTACATT 
GATGAGGCTC ACCCATCAGA TGGCTGGGCT GCTCCTGGTA TCTCCCCCTC TTCCTTTGAA 
GTTAAGAAGC ACAGGAATCA GGAAGATCGA TGTGCAGCTG CTCACCAGCT CCTGGAACGC 
TTCTCCTTGC CACCTCAGTG CCAAGTGGTG GCTGACTGCA TGGACAACAA TGCCAATGTG 
GCCTATGGGG TTTCATTTGA GCGAGTGTGC ATTGTGCAGA GACAAAAAAT TGCCTACCTG 
GGAGGTAAAG GCCCCTTTTT CTACAATCTT CAGGAGGTTC GTCTTTGGTT GGAACAAAAC 
TTCAGCAAAA GATGAAATCC ATTGTCTACA GAAGATTTGT CAACAGATGT GTCCCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989445.2
CDS	594..1433
Translation	MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHMVLFLSRSKSARGEWRRMLTSEGLRCVWNSFLLDAYKQVKLGGEAPNSSVIHIAKGNDGSNSSWKSVGGKCGTKCHLLDFANSERPLVVNFGSATPPFTSQLSAFSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLEQNFSKRNPLSTEDLSTDVSL

Target ORF information:

RefSeq Version	NM_204114.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus iodothyronine deiodinase 2 (DIO2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204114.3

ORF Insert Sequence:

ATGGGTCTGT TAAGTGTGGA TTTGCTGATC ACGCTTCAGA TCTTGCCGGT CTTTTTCTCC 
AATTGCCTCT TCCTTGCGCT GTATGACTCT GTGATCCTCC TGAAGCACAT GGTGCTGTTT 
CTGAGCCGCT CCAAGTCTGC GCGCGGTGAG TGGCGGAGGA TGCTGACCTC GGAGGGGCTG 
CGCTGCGTCT GGAACAGCTT CCTCCTGGAC GCCTACAAGC AGGTCAAACT TGGAGGAGAA 
GCTCCGAACT CCAGTGTAAT CCACATAGCC AAGGGCAATG ATGGCAGCAA TAGCAGCTGG 
AAGAGTGTTG GTGGCAAGTG TGGAACCAAG TGCCATCTTC TGGATTTTGC CAACTCTGAG 
CGGCCACTGG TGGTTAATTT TGGCTCAGCT ACCTGACCAC CATTCACAAG CCAGCTGTCT 
GCCTTCAGCA AGCTGGTGGA AGAGTTCTCT GGCGTGGCTG ACTTTCTGTT GGTCTACATT 
GATGAGGCTC ACCCATCAGA TGGCTGGGCT GCTCCTGGTA TCTCCCCCTC TTCCTTTGAA 
GTTAAGAAGC ACAGGAATCA GGAAGATCGA TGTGCAGCTG CTCACCAGCT CCTGGAACGC 
TTCTCCTTGC CACCTCAGTG CCAAGTGGTG GCTGACTGCA TGGACAACAA TGCCAATGTG 
GCCTATGGGG TTTCATTTGA GCGAGTGTGC ATTGTGCAGA GACAAAAAAT TGCCTACCTG 
GGAGGTAAAG GCCCCTTTTT CTACAATCTT CAGGAGGTTC GTCTTTGGTT GGAACAAAAC 
TTCAGCAAAA GATGAAATCC ATTGTCTACA GAAGATTTGT CAACAGATGT GTCCCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIO2 ( NM_204114.4 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989445.3 Gallus gallus deiodinase, iodothyronine type II (DIO2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OGa153300
Clone ID Related Accession (Same CDS sequence)	NM_204114.4
Accession Version	NM_204114.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Gallus gallus(chicken)
Product	type II iodothyronine deiodinase isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000303.1. On Jun 16, 2018 this sequence version replaced NM_204114.3. Transcript Variant: This variant (1) represents the canonical 1-Sec containing isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF125575.1, BU369644.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201357, SAMEA2201359 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 10318780 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGTCTGT TAAGTGTGGA TTTGCTGATC ACGCTTCAGA TCTTGCCGGT CTTTTTCTCC 
AATTGCCTCT TCCTTGCGCT GTATGACTCT GTGATCCTCC TGAAGCACAT GGTGCTGTTT 
CTGAGCCGCT CCAAGTCTGC GCGCGGTGAG TGGCGGAGGA TGCTGACCTC GGAGGGGCTG 
CGCTGCGTCT GGAACAGCTT CCTCCTGGAC GCCTACAAGC AGGTCAAACT TGGAGGAGAA 
GCTCCGAACT CCAGTGTAAT CCACATAGCC AAGGGCAATG ATGGCAGCAA TAGCAGCTGG 
AAGAGTGTTG GTGGCAAGTG TGGAACCAAG TGCCATCTTC TGGATTTTGC CAACTCTGAG 
CGGCCACTGG TGGTTAATTT TGGCTCAGCT ACCTGACCAC CATTCACAAG CCAGCTGTCT 
GCCTTCAGCA AGCTGGTGGA AGAGTTCTCT GGCGTGGCTG ACTTTCTGTT GGTCTACATT 
GATGAGGCTC ACCCATCAGA TGGCTGGGCT GCTCCTGGTA TCTCCCCCTC TTCCTTTGAA 
GTTAAGAAGC ACAGGAATCA GGAAGATCGA TGTGCAGCTG CTCACCAGCT CCTGGAACGC 
TTCTCCTTGC CACCTCAGTG CCAAGTGGTG GCTGACTGCA TGGACAACAA TGCCAATGTG 
GCCTATGGGG TTTCATTTGA GCGAGTGTGC ATTGTGCAGA GACAAAAAAT TGCCTACCTG 
GGAGGTAAAG GCCCCTTTTT CTACAATCTT CAGGAGGTTC GTCTTTGGTT GGAACAAAAC 
TTCAGCAAAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989445.3
CDS	594..1388
Translation	MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHMVLFLSRSKSARGEWRRMLTSEGLRCVWNSFLLDAYKQVKLGGEAPNSSVIHIAKGNDGSNSSWKSVGGKCGTKCHLLDFANSERPLVVNFGSAT*PPFTSQLSAFSKLVEEFSGVADFLLVYIDEAHPSDGWAAPGISPSSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADCMDNNANVAYGVSFERVCIVQRQKIAYLGGKGPFFYNLQEVRLWLEQNFSKR

Target ORF information:

RefSeq Version	NM_204114.4
Organism	Gallus gallus(chicken)
Definition	Gallus gallus deiodinase, iodothyronine type II (DIO2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204114.4

ORF Insert Sequence:

ATGGGTCTGT TAAGTGTGGA TTTGCTGATC ACGCTTCAGA TCTTGCCGGT CTTTTTCTCC 
AATTGCCTCT TCCTTGCGCT GTATGACTCT GTGATCCTCC TGAAGCACAT GGTGCTGTTT 
CTGAGCCGCT CCAAGTCTGC GCGCGGTGAG TGGCGGAGGA TGCTGACCTC GGAGGGGCTG 
CGCTGCGTCT GGAACAGCTT CCTCCTGGAC GCCTACAAGC AGGTCAAACT TGGAGGAGAA 
GCTCCGAACT CCAGTGTAAT CCACATAGCC AAGGGCAATG ATGGCAGCAA TAGCAGCTGG 
AAGAGTGTTG GTGGCAAGTG TGGAACCAAG TGCCATCTTC TGGATTTTGC CAACTCTGAG 
CGGCCACTGG TGGTTAATTT TGGCTCAGCT ACCTGACCAC CATTCACAAG CCAGCTGTCT 
GCCTTCAGCA AGCTGGTGGA AGAGTTCTCT GGCGTGGCTG ACTTTCTGTT GGTCTACATT 
GATGAGGCTC ACCCATCAGA TGGCTGGGCT GCTCCTGGTA TCTCCCCCTC TTCCTTTGAA 
GTTAAGAAGC ACAGGAATCA GGAAGATCGA TGTGCAGCTG CTCACCAGCT CCTGGAACGC 
TTCTCCTTGC CACCTCAGTG CCAAGTGGTG GCTGACTGCA TGGACAACAA TGCCAATGTG 
GCCTATGGGG TTTCATTTGA GCGAGTGTGC ATTGTGCAGA GACAAAAAAT TGCCTACCTG 
GGAGGTAAAG GCCCCTTTTT CTACAATCTT CAGGAGGTTC GTCTTTGGTT GGAACAAAAC 
TTCAGCAAAA GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIO2 ( NM_001324555.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001311484.1 Gallus gallus deiodinase, iodothyronine type II (DIO2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OGa86423
Clone ID Related Accession (Same CDS sequence)	NM_001324555.2 , NM_001324555.1
Accession Version	NM_001324555.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Gallus gallus(chicken)
Product	type II iodothyronine deiodinase isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN05000303.1. On Jun 16, 2018 this sequence version replaced NM_001324555.1. Transcript Variant: This variant (2) uses an alternate donor splice site, which results in a frameshift compared to variant 1. The resulting isoform (b) is shorter, with a distinct C-terminus compared to isoform a. Isoform b lacks the Sec residue found at the active site; thus, is inactive (PMID:12089359). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF401753.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCTGT TAAGTGTGGA TTTGCTGATC ACGCTTCAGA TCTTGCCGGT CTTTTTCTCC 
AATTGCCTCT TCCTTGCGCT GTATGACTCT GTGATCCTCC TGAAGCACAT GGTGCTGTTT 
CTGAGCCGCT CCAAGTCTGC GCGCGGTCAA ACTTGGAGGA GAAGCTCCGA ACTCCAGTGT 
AATCCACATA GCCAAGGGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311484.1
CDS	1..204
Translation	MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHMVLFLSRSKSARGQTWRRSSELQCNPHSQGQ

Target ORF information:

RefSeq Version	NM_001324555.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus deiodinase, iodothyronine type II (DIO2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324555.2

ORF Insert Sequence:

ATGGGTCTGT TAAGTGTGGA TTTGCTGATC ACGCTTCAGA TCTTGCCGGT CTTTTTCTCC 
AATTGCCTCT TCCTTGCGCT GTATGACTCT GTGATCCTCC TGAAGCACAT GGTGCTGTTT 
CTGAGCCGCT CCAAGTCTGC GCGCGGTCAA ACTTGGAGGA GAAGCTCCGA ACTCCAGTGT 
AATCCACATA GCCAAGGGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIO2 ( NM_001324555.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001311484.1 Gallus gallus iodothyronine deiodinase 2 (DIO2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OGa86423
Clone ID Related Accession (Same CDS sequence)	NM_001324555.2 , NM_001324555.1
Accession Version	NM_001324555.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-09
Organism	Gallus gallus(chicken)
Product	type II iodothyronine deiodinase isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AADN04000015.1. Transcript Variant: This variant (2) uses an alternate donor splice site, which results in a frameshift compared to variant 1. The resulting isoform (b) is shorter, with a distinct C-terminus compared to isoform a. Isoform b lacks the selenocysteine residue found at the active site; thus is inactive (PMID:12089359). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF401753.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2812694 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCTGT TAAGTGTGGA TTTGCTGATC ACGCTTCAGA TCTTGCCGGT CTTTTTCTCC 
AATTGCCTCT TCCTTGCGCT GTATGACTCT GTGATCCTCC TGAAGCACAT GGTGCTGTTT 
CTGAGCCGCT CCAAGTCTGC GCGCGGTCAA ACTTGGAGGA GAAGCTCCGA ACTCCAGTGT 
AATCCACATA GCCAAGGGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311484.1
CDS	1..204
Translation	MGLLSVDLLITLQILPVFFSNCLFLALYDSVILLKHMVLFLSRSKSARGQTWRRSSELQCNPHSQGQ

Target ORF information:

RefSeq Version	NM_001324555.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus iodothyronine deiodinase 2 (DIO2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324555.1

ORF Insert Sequence:

ATGGGTCTGT TAAGTGTGGA TTTGCTGATC ACGCTTCAGA TCTTGCCGGT CTTTTTCTCC 
AATTGCCTCT TCCTTGCGCT GTATGACTCT GTGATCCTCC TGAAGCACAT GGTGCTGTTT 
CTGAGCCGCT CCAAGTCTGC GCGCGGTCAA ACTTGGAGGA GAAGCTCCGA ACTCCAGTGT 
AATCCACATA GCCAAGGGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Detection of long-term influence of prenatal temperature stimulation on hypothalamic type-II iodothyronine deiodinase in juvenile female broiler chickens using a novel immunohistochemical amplification protocol.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology179120-4(2015 Jan)
Nassar M,Halle I,Plagemann A,Tzschentke B

Dynamic mRNA distribution pattern of thyroid hormone transporters and deiodinases during early embryonic chicken brain development.
Neuroscience22169-85(2012 Sep)
Geysens S,Ferran JL,Van Herck SL,Tylzanowski P,Puelles L,Darras VM

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

Differential expression of iodothyronine deiodinases in chicken tissues during the last week of embryonic development.
General and comparative endocrinology128(1)65-73(2002 Aug)
Van der Geyten S,Van den Eynde I,Segers IB,K?hn ER,Darras VM

The mRNA structure has potent regulatory effects on type 2 iodothyronine deiodinase expression.
Molecular endocrinology (Baltimore, Md.)16(7)1667-79(2002 Jul)
Gereben B,Koll?r A,Harney JW,Larsen PR

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DIO2 cDNA ORF clone, Gallus gallus(chicken)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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