dio2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dio2
Entrez Gene ID	352937
Full Name	iodothyronine deiodinase 2
Synonyms	Deio2,cb684
General protein information	Preferred Names iodothyronine deiodinase 2 Names type II iodothyronine deiodinase D2 deiodinase, iodothyronine, type II
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination. This enzyme is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in those peripheral tissues where it is expressed. Knockdown studies in zebrafish embryos indicate that this gene is essential for thyroid hormone-dependent embryonic development and pigmentation in zebrafish. This protein is a selenoprotein containing the rare selenocysteine (Sec) amino acid at its active site, and may contain additional Sec residues. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, May 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_212789.4	NP_997954.4	type II iodothyronine deiodinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIO2	NP_001007024.1
Bos taurus (cattle)	DIO2	NP_001010992.1
Danio rerio (zebrafish)	dio2	NP_997954.2
Xenopus tropicalis (tropical clawed frog)	dio2	NP_001184161.2
Pan troglodytes (chimpanzee)	DIO2	NP_001116109.1
Canis lupus familiaris (dog)	DIO2	NP_001116117.1
	DIO2	NP_001152766.1
Mus musculus (house mouse)	Dio2	NP_034180.1
Rattus norvegicus (Norway rat)	Dio2	NP_113908.3
Gallus gallus (chicken)	DIO2	NP_989445.2

Gene Ontology
Bibliography

Related articles in PubMed

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

essential to assure normal development
Title: Permanent Deiodinase Type 2 Deficiency Strongly Perturbs Zebrafish Development, Growth, and Fertility.

Knockdown of D1+D2 (D1D2MO) and knockdown of D3 (D3MO) both resulted in transcriptional regulation of energy metabolism and (muscle) development in abdomen and tail
Title: Deiodinase knockdown during early zebrafish development affects growth, development, energy metabolism, motility and phototransduction.

This study investigated deiodinase 1 (Dio1), deiodinase 2 (Dio2), and deiodinase 3 (Dio3) mRNA expression at the several zebrafish life stages and found life stage specific expression of these genes that were highly localized.
Title: Using whole mount in situ hybridization to examine thyroid hormone deiodinase expression in embryonic and larval zebrafish: a tool for examining OH-BDE toxicity to early life stages.

role in zebrafish embryonic development
Title: Combined antisense knockdown of type 1 and type 2 iodothyronine deiodinases disrupts embryonic development in zebrafish (Danio rerio).

The following dio2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dio2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00691	NM_212789.4 Latest version!	Danio rerio iodothyronine deiodinase 2 (dio2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dio2 ( NM_212789.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997954.4 Danio rerio iodothyronine deiodinase 2 (dio2), mRNA.

Price & Availability↑

CloneID	ODa00691
Clone ID Related Accession (Same CDS sequence)	NM_212789.4
Accession Version	NM_212789.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Danio rerio(zebrafish)
Product	type II iodothyronine deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EB921177.1, BC059608.1, EE323348.1 and EB875967.1. On May 13, 2016 this sequence version replaced NM_212789.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC059608.1, GDQQ01000117.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 12915322 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCTTGC TTAGTGTGGA CCTCCTGGTA ACTTTGCAGA TTTTGCCTGG CTTCTTCTCC 
AACTGCCTGT TTTTCGTGCT GTATGACTCG ATAGTGCTGG TTAAGCGTGT GGTGTCACTC 
CTGAGCTGCT CGGGCTCCAC GGGTGAGTGG CAGCGCATGT TAACCACAGC CGGCGTGCGC 
TCCATATGGA ACAGCTTTCT TCTGGATGCC TACAAACAGG TGAAATTGGG CGAAGCTGCT 
CCAAACTCTA AGGTGGTGAA AGTCACTGGC ATCAATAGAT GCTGGAGCAT CAGCGGTAAG 
ACCCACAACC AGTGCCACCT GCTGGACTTT GAGTCTCCTG ATCGCCCGTT AGTGGTCAAC 
TTCGGTTCGG CCACTTGACC CCCCTTTATA AGCCAGCTGC CGGTCTTCCG GCGGATGGTG 
GAGGAATTTT CAGATGTGGC AGATTTCCTG CTGGTCTACA TCGATGAGGC TCACCCTTCA 
GACGGCTGGG TGGGTCCTCC CATGGAGAAC TTTTCGTTTG AAGTGAGGAA ACACCGAAAT 
CTGGAGGAGA GGATGTTTGC CGCCAGGACA CTTCTGGAGC ATTTCTCCTT GCCTCCTCAG 
TGCCAGTTGG TGGCCGATTG CATGGACAAT AATGCCAACA TAGCCTACGG TGTGTCCTAC 
GAGAGGGTCT GCATCGTGCA GAAGAATAAA ATCGCTTACC TTGGGGGTAA GGGTCCATTT 
TTTTACAACC TTAAAGATGT TCGGAGGTGG CTGGAGAAGT GCTACGGAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997954.4
CDS	305..1078
Translation	MGLLSVDLLVTLQILPGFFSNCLFFVLYDSIVLVKRVVSLLSCSGSTGEWQRMLTTAGVRSIWNSFLLDAYKQVKLGEAAPNSKVVKVTGINRCWSISGKTHNQCHLLDFESPDRPLVVNFGSAT*PPFISQLPVFRRMVEEFSDVADFLLVYIDEAHPSDGWVGPPMENFSFEVRKHRNLEERMFAARTLLEHFSLPPQCQLVADCMDNNANIAYGVSYERVCIVQKNKIAYLGGKGPFFYNLKDVRRWLEKCYGK

Target ORF information:

RefSeq Version	NM_212789.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio iodothyronine deiodinase 2 (dio2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212789.4

ORF Insert Sequence:

ATGGGCTTGC TTAGTGTGGA CCTCCTGGTA ACTTTGCAGA TTTTGCCTGG CTTCTTCTCC 
AACTGCCTGT TTTTCGTGCT GTATGACTCG ATAGTGCTGG TTAAGCGTGT GGTGTCACTC 
CTGAGCTGCT CGGGCTCCAC GGGTGAGTGG CAGCGCATGT TAACCACAGC CGGCGTGCGC 
TCCATATGGA ACAGCTTTCT TCTGGATGCC TACAAACAGG TGAAATTGGG CGAAGCTGCT 
CCAAACTCTA AGGTGGTGAA AGTCACTGGC ATCAATAGAT GCTGGAGCAT CAGCGGTAAG 
ACCCACAACC AGTGCCACCT GCTGGACTTT GAGTCTCCTG ATCGCCCGTT AGTGGTCAAC 
TTCGGTTCGG CCACTTGACC CCCCTTTATA AGCCAGCTGC CGGTCTTCCG GCGGATGGTG 
GAGGAATTTT CAGATGTGGC AGATTTCCTG CTGGTCTACA TCGATGAGGC TCACCCTTCA 
GACGGCTGGG TGGGTCCTCC CATGGAGAAC TTTTCGTTTG AAGTGAGGAA ACACCGAAAT 
CTGGAGGAGA GGATGTTTGC CGCCAGGACA CTTCTGGAGC ATTTCTCCTT GCCTCCTCAG 
TGCCAGTTGG TGGCCGATTG CATGGACAAT AATGCCAACA TAGCCTACGG TGTGTCCTAC 
GAGAGGGTCT GCATCGTGCA GAAGAATAAA ATCGCTTACC TTGGGGGTAA GGGTCCATTT 
TTTTACAACC TTAAAGATGT TCGGAGGTGG CTGGAGAAGT GCTACGGAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

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dio2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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