DIO2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DIO2
Entrez Gene ID	494548
Full Name	iodothyronine deiodinase 2
Synonyms	5DII,DIOII
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Summary	The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination. This gene is widely expressed, with most abundant expression in thyroid, mammary and pituitary glands. It is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues. This protein is a selenoprotein containing the rare selenocysteine (Sec) amino acid at its active site, and may contain additional Sec residues. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, May 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001010992.5	NP_001010992.1	type II iodothyronine deiodinase
NM_001010992.7	NP_001010992.2	type II iodothyronine deiodinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIO2	NP_001007024.1
Bos taurus (cattle)	DIO2	NP_001010992.1
Danio rerio (zebrafish)	dio2	NP_997954.2
Xenopus tropicalis (tropical clawed frog)	dio2	NP_001184161.2
Pan troglodytes (chimpanzee)	DIO2	NP_001116109.1
Canis lupus familiaris (dog)	DIO2	NP_001116117.1
	DIO2	NP_001152766.1
Mus musculus (house mouse)	Dio2	NP_034180.1
Rattus norvegicus (Norway rat)	Dio2	NP_113908.3
Gallus gallus (chicken)	DIO2	NP_989445.2

Gene Ontology
Bibliography

Related articles in PubMed

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Regulation of mammary gland sensitivity to thyroid hormones during the transition from pregnancy to lactation.
Capuco AV, Connor EE, Wood DL
Experimental biology and medicine (Maywood, N.J.)233(10)1309-14(2008 Oct)

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
Jann OC, Aerts J, Jones M, Hastings N, Law A, McKay S, Marques E, Prasad A, Yu J, Moore SS, Floriot S, Mah? MF, Eggen A, Silveri L, Negrini R, Milanesi E, Ajmone-Marsan P, Valentini A, Marchitelli C, Savarese MC, Janitz M, Herwig R, Hennig S, Gorni C, Connor EE, Sonstegard TS, Smith T, Dr?gem?ller C, Williams JL
BMC genomics7283(2006 Nov)

Molecular cloning, expression and radiation hybrid mapping of the bovine deiodinase type II (DIO2) and deiodinase type III (DIO3) genes.
Connor EE, Laiakis EC, Fernandes VM, Williams JL, Capuco AV
Animal genetics36(3)240-3(2005 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increased expression of mammary TRbeta1 and DIO2, and decreased RXRalpha, provide a mechanism to increase thyroid hormone activity within the mammary gland during lactation.
Title: Regulation of mammary gland sensitivity to thyroid hormones during the transition from pregnancy to lactation.

The following DIO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa44021	NM_001010992.5 Latest version!	Bos taurus iodothyronine deiodinase 2 (DIO2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa239214	NM_001010992.7 Latest version!	Bos taurus iodothyronine deiodinase 2 (DIO2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DIO2 ( NM_001010992.5 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001010992.1 Bos taurus iodothyronine deiodinase 2 (DIO2), mRNA.

Price & Availability↑

CloneID	OBa44021
Clone ID Related Accession (Same CDS sequence)	NM_001010992.5
Accession Version	NM_001010992.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	type II iodothyronine deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY858551.2 and DAAA02029819.1. On May 11, 2016 this sequence version replaced NM_001010992.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY858551.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02822095, SAMN02822097 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 15932406 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCATCC TCAGCGTAGA CTTGCTGATC ACACTGCAGA TTCTGCCAGT TTTTTTCTCC 
AACTGCCTCT TCCTGGCGCT CTATGACTCG GTCATTCTCC TCAAGCACGT GGTGCTGCTG 
CTGAGCCGCT CCAAGTCCAC TCGCGGGCAG TGGAGGCGCA TGCTGACCTC AGAAGGAATG 
CGCTGCATCT GGAAAAGCTT CCTCCTCGAC GCCTACAAAC AGGTGAAACT GGGTGAAGAT 
GCCCCCAATT CCAGCGTGGT GCATGTCTCC AGTCCGGAAG GAGGTGACAC CAGTGGAAAT 
GGTGCCCAGG AGAAAACAGT GGATGGAACT GAATGCCACC TTCTGGACTT TGCCAGCCCT 
GAGCGCCCAC TGGTGGTCAA CTTCGGCTCG GCCACTTGAC CTCCTTTCAC TAACCAGCTG 
CCAGCCTTCA GCAAACTGGT GGAAGAGTTC TCATCCGTGG CTGACTTCCT GTTGGTCTAC 
ATTGATGAGG CTCATCCTTC AGATGGTTGG GCAGTGCCTG GGGATTCTTC TTTGTTTTTC 
GAGGTGAAGA AGCACCGGAA CCAGGAAGAC CGATGCGCAG CAGCCCACCA GCTCCTGGAG 
CGTTTCTCCT TGCCGCCCCA GTGCCGAGTT GTGGCTGACC GCATGGACAA TAATGCCAAC 
GTTGCTTATG GGGTAGCCTT TGAACGTGTG TGCATCGTGC AGAGACAGAA AATTGCTTAT 
CTGGGAGGAA AGGGCCCCTT CTTTTACAAC CTGCAAGAAG TCCGGCGTTG GCTGGAGAAG 
AATTTCAGCA AGAGATGAAA GCTAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001010992.1
CDS	11..820
Translation	MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGQWRRMLTSEGMRCIWKSFLLDAYKQVKLGEDAPNSSVVHVSSPEGGDTSGNGAQEKTVDGTECHLLDFASPERPLVVNFGSATPPFTNQLPAFSKLVEEFSSVADFLLVYIDEAHPSDGWAVPGDSSLFFEVKKHRNQEDRCAAAHQLLERFSLPPQCRVVADRMDNNANVAYGVAFERVCIVQRQKIAYLGGKGPFFYNLQEVRRWLEKNFSKRKLD

Target ORF information:

RefSeq Version	NM_001010992.5
Organism	Bos taurus(cattle)
Definition	Bos taurus iodothyronine deiodinase 2 (DIO2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001010992.5

ORF Insert Sequence:

ATGGGCATCC TCAGCGTAGA CTTGCTGATC ACACTGCAGA TTCTGCCAGT TTTTTTCTCC 
AACTGCCTCT TCCTGGCGCT CTATGACTCG GTCATTCTCC TCAAGCACGT GGTGCTGCTG 
CTGAGCCGCT CCAAGTCCAC TCGCGGGCAG TGGAGGCGCA TGCTGACCTC AGAAGGAATG 
CGCTGCATCT GGAAAAGCTT CCTCCTCGAC GCCTACAAAC AGGTGAAACT GGGTGAAGAT 
GCCCCCAATT CCAGCGTGGT GCATGTCTCC AGTCCGGAAG GAGGTGACAC CAGTGGAAAT 
GGTGCCCAGG AGAAAACAGT GGATGGAACT GAATGCCACC TTCTGGACTT TGCCAGCCCT 
GAGCGCCCAC TGGTGGTCAA CTTCGGCTCG GCCACTTGAC CTCCTTTCAC TAACCAGCTG 
CCAGCCTTCA GCAAACTGGT GGAAGAGTTC TCATCCGTGG CTGACTTCCT GTTGGTCTAC 
ATTGATGAGG CTCATCCTTC AGATGGTTGG GCAGTGCCTG GGGATTCTTC TTTGTTTTTC 
GAGGTGAAGA AGCACCGGAA CCAGGAAGAC CGATGCGCAG CAGCCCACCA GCTCCTGGAG 
CGTTTCTCCT TGCCGCCCCA GTGCCGAGTT GTGGCTGACC GCATGGACAA TAATGCCAAC 
GTTGCTTATG GGGTAGCCTT TGAACGTGTG TGCATCGTGC AGAGACAGAA AATTGCTTAT 
CTGGGAGGAA AGGGCCCCTT CTTTTACAAC CTGCAAGAAG TCCGGCGTTG GCTGGAGAAG 
AATTTCAGCA AGAGATGAAA GCTAGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIO2 ( NM_001010992.7 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001010992.2 Bos taurus iodothyronine deiodinase 2 (DIO2), mRNA.

Price & Availability↑

CloneID	OBa239214
Clone ID Related Accession (Same CDS sequence)	NM_001010992.7
Accession Version	NM_001010992.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Bos taurus(cattle)
Product	type II iodothyronine deiodinase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY858551.2, CB172450.1, NKLS02000010.1, CB462845.1, CB464288.1, DV884035.1, EE344691.1, CB454879.1 and CO884182.1. On Oct 9, 2018 this sequence version replaced NM_001010992.6. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.7579.1, SRR6764281.8520.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145416 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 15932406 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCATCC TCAGCGTAGA CTTGCTGATC ACACTGCAGA TTCTGCCAGT TTTTTTCTCC 
AACTGCCTCT TCCTGGCGCT CTATGACTCG GTCATTCTCC TCAAGCACGT GGTGCTGCTG 
CTGAGCCGCT CCAAGTCCAC TCGCGGGCAG TGGAGGCGCA TGCTGACCTC AGAAGGAATG 
CGCTGCATCT GGAAAAGCTT CCTCCTCGAC GCCTACAAAC AGGTGAAACT GGGTGAAGAT 
GCCCCCAATT CCAGCGTGGT GCATGTCTCC AGTCCGGAAG GAGGTGACAC CAGTGGAAAT 
GGTGCCCAGG AGAAAACAGT GGATGGAACT GAATGCCACC TTCTGGACTT TGCCAGCCCT 
GAGCGCCCAC TGGTGGTCAA CTTCGGCTCG GCCACTTGAC CTCCTTTCAC TAACCAGCTG 
CCAGCCTTCA GCAAACTGGT GGAAGAGTTC TCATCCGTGG CTGACTTCCT GTTGGTCTAC 
ATTGATGAGG CTCATCCTTC AGATGGTTGG GCAGTGCCTG GGGATTCTTC TTTGTTTTTC 
GAGGTGAAGA AGCACCGGAA CCAGGAAGAC CGATGCGCAG CAGCCCACCA GCTCCTGGAG 
CGTTTCTCCT TGCCGCCCCA GTGCCGAGTT GTGGCTGACC GCATGGACAA TAATGCCAAC 
GTTGCTTATG GGGTAGCCTT TGAACGTGTG TGCATCGTGC AGAGACAGAA AATTGCTTAT 
CTGGGAGGAA AGGGCCCCTT CTTTTACAAC CTGCAAGAAG TCCGGCGTTG GCTGGAGAAG 
AATTTCAGCA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001010992.2
CDS	11..808
Translation	MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGQWRRMLTSEGMRCIWKSFLLDAYKQVKLGEDAPNSSVVHVSSPEGGDTSGNGAQEKTVDGTECHLLDFASPERPLVVNFGSAT*PPFTNQLPAFSKLVEEFSSVADFLLVYIDEAHPSDGWAVPGDSSLFFEVKKHRNQEDRCAAAHQLLERFSLPPQCRVVADRMDNNANVAYGVAFERVCIVQRQKIAYLGGKGPFFYNLQEVRRWLEKNFSKR

Target ORF information:

RefSeq Version	NM_001010992.7
Organism	Bos taurus(cattle)
Definition	Bos taurus iodothyronine deiodinase 2 (DIO2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001010992.7

ORF Insert Sequence:

ATGGGCATCC TCAGCGTAGA CTTGCTGATC ACACTGCAGA TTCTGCCAGT TTTTTTCTCC 
AACTGCCTCT TCCTGGCGCT CTATGACTCG GTCATTCTCC TCAAGCACGT GGTGCTGCTG 
CTGAGCCGCT CCAAGTCCAC TCGCGGGCAG TGGAGGCGCA TGCTGACCTC AGAAGGAATG 
CGCTGCATCT GGAAAAGCTT CCTCCTCGAC GCCTACAAAC AGGTGAAACT GGGTGAAGAT 
GCCCCCAATT CCAGCGTGGT GCATGTCTCC AGTCCGGAAG GAGGTGACAC CAGTGGAAAT 
GGTGCCCAGG AGAAAACAGT GGATGGAACT GAATGCCACC TTCTGGACTT TGCCAGCCCT 
GAGCGCCCAC TGGTGGTCAA CTTCGGCTCG GCCACTTGAC CTCCTTTCAC TAACCAGCTG 
CCAGCCTTCA GCAAACTGGT GGAAGAGTTC TCATCCGTGG CTGACTTCCT GTTGGTCTAC 
ATTGATGAGG CTCATCCTTC AGATGGTTGG GCAGTGCCTG GGGATTCTTC TTTGTTTTTC 
GAGGTGAAGA AGCACCGGAA CCAGGAAGAC CGATGCGCAG CAGCCCACCA GCTCCTGGAG 
CGTTTCTCCT TGCCGCCCCA GTGCCGAGTT GTGGCTGACC GCATGGACAA TAATGCCAAC 
GTTGCTTATG GGGTAGCCTT TGAACGTGTG TGCATCGTGC AGAGACAGAA AATTGCTTAT 
CTGGGAGGAA AGGGCCCCTT CTTTTACAAC CTGCAAGAAG TCCGGCGTTG GCTGGAGAAG 
AATTTCAGCA AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Regulation of mammary gland sensitivity to thyroid hormones during the transition from pregnancy to lactation.
Experimental biology and medicine (Maywood, N.J.)233(10)1309-14(2008 Oct)
Capuco AV,Connor EE,Wood DL

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
BMC genomics7283(2006 Nov)
Jann OC,Aerts J,Jones M,Hastings N,Law A,McKay S,Marques E,Prasad A,Yu J,Moore SS,Floriot S,Mah? MF,Eggen A,Silveri L,Negrini R,Milanesi E,Ajmone-Marsan P,Valentini A,Marchitelli C,Savarese MC,Janitz M,Herwig R,Hennig S,Gorni C,Connor EE,Sonstegard TS,Smith T,Dr?gem?ller C,Williams JL

Molecular cloning, expression and radiation hybrid mapping of the bovine deiodinase type II (DIO2) and deiodinase type III (DIO3) genes.
Animal genetics36(3)240-3(2005 Jun)
Connor EE,Laiakis EC,Fernandes VM,Williams JL,Capuco AV

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DIO2 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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