Acaca cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acaca
Entrez Gene ID	60581
Full Name	acetyl-CoA carboxylase alpha
Synonyms	ACC1,Acac
General protein information	Preferred Names acetyl-CoA carboxylase alpha Names acetyl-CoA carboxylase 1 ACC-alpha acetyl-coenzyme A carboxylase alpha
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q26
Summary	catalyzes the rate-limiting carboxylation of acetyl CoA to malonyl CoA during long-chain fatty acid synthesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022193.1	NP_071529.1	acetyl-CoA carboxylase 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ACACA	XP_005624832.1
Bos taurus (cattle)	ACACA	NP_776649.1
Mus musculus (house mouse)	Acaca	NP_579938.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005175	XP_314071.4
Schizosaccharomyces pombe (fission yeast)	cut6	NP_593271.1
Arabidopsis thaliana (thale cress)	ACC1	NP_174849.2
	ACACA	XP_001111823.1
Rattus norvegicus (Norway rat)	Acaca	NP_071529.1
Magnaporthe oryzae (rice blast fungus)	MGG_07613	XP_003711534.1
Drosophila melanogaster (fruit fly)	ACC	NP_724636.1
Saccharomyces cerevisiae (baker's yeast)	ACC1	NP_014413.1
Eremothecium gossypii	AGOS_AAR071W	NP_982612.1
Neurospora crassa	NCU08535	XP_963017.1
Pan troglodytes (chimpanzee)	ACACA	XP_511428.3
Gallus gallus (chicken)	ACACA	NP_990836.1
Arabidopsis thaliana (thale cress)	ACC2	NP_174850.4
Homo sapiens (human)	ACACA	NP_942131.1
Danio rerio (zebrafish)	acaca	NP_001258237.1
Kluyveromyces lactis	KLLA0F06072g	XP_455355.1
Xenopus tropicalis (tropical clawed frog)	acaca	XP_004911956.1

Gene Ontology
Bibliography

Related articles in PubMed

Obesity-alleviating potential of asiatic acid and its effects on ACC1, UCP2, and CPT1 mRNA expression in high fat diet-induced obese Sprague-Dawley rats.
Rameshreddy P, Uddandrao VVS, Brahmanaidu P, Vadivukkarasi S, Ravindarnaik R, Suresh P, Swapna K, Kalaivani A, Parvathi P, Tamilmani P, Saravanan G
Molecular and cellular biochemistry(2017 Oct)

Liver X Receptor Agonist TO901317 Prevents Diacylglycerols Accumulation in the Heart of Streptozotocin-Diabetic Rats.
Harasiuk D, Baranowski M, Zabielski P, Chabowski A, G?rski J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology39(1)350-9(2016)

Selective impairment of P2Y signaling by prostaglandin E2 in macrophages: implications for Ca2+-dependent responses.
Trav?s PG, Pimentel-Santillana M, Carrasquero LM, P?rez-Sen R, Delicado EG, Luque A, Izquierdo M, Mart?n-Sanz P, Miras-Portugal MT, Bosc? L
Journal of immunology (Baltimore, Md. : 1950)190(8)4226-35(2013 Apr)

Changes in white adipose tissue metabolism induced by resveratrol in rats.
Alberdi G, Rodr?guez VM, Miranda J, Macarulla MT, Arias N, Andr?s-Lacueva C, Portillo MP
Nutrition & metabolism8(1)29(2011 May)

Crystal structure of Spot 14, a modulator of fatty acid synthesis.
Colbert CL, Kim CW, Moon YA, Henry L, Palnitkar M, McKean WB, Fitzgerald K, Deisenhofer J, Horton JD, Kwon HJ
Proceedings of the National Academy of Sciences of the United States of America107(44)18820-5(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that TO901317 increased expression of both Liver X receptors (LXRalpha and LXRbeta) isoforms and its target genes: sterol response element binding protein 1c (SREBP-1c) and acetyl-coenzyme A carboxylase 1 (ACC1) in the heart of streptozotocin-diabetes.
Title: Liver X Receptor Agonist TO901317 Prevents Diacylglycerols Accumulation in the Heart of Streptozotocin-Diabetic Rats.

data support a potential role for Acetyl-CoA carboxylases (ACC) 1 and 2 in the development of obesity and diabetes in rats; results point towards major differences in ACC tissue distribution between humans and rats
Title: Acetyl-CoA carboxylases 1 and 2 show distinct expression patterns in rats and humans and alterations in obesity and diabetes.

The citrate produced by mitochondria may modulate ACC phosphorylation in the hypothalamus, controlling food intake and coordinating a multiorgan network that controls glucose homeostasis and energy uptake through the adrenergic system.
Title: Citrate diminishes hypothalamic acetyl-CoA carboxylase phosphorylation and modulates satiety signals and hepatic mechanisms involved in glucose homeostasis in rats.

suppression of ACC1 activity impairs glucose-stimulated insulin secretion via inhibition of glucose rather than lipid metabolism.
Title: Chronic suppression of acetyl-CoA carboxylase 1 in beta-cells impairs insulin secretion via inhibition of glucose rather than lipid metabolism.

Consistent with pathways of hepatic triglyceride synthesis, expression of diacylglycerol acyltransferase-1 and -2 was increased, as were delta-5- and delta-6- fatty acid desaturase mRNA levels.
Title: Hepatic free fatty acids accumulate in experimental steatohepatitis: role of adaptive pathways.

The following Acaca gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acaca cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05402	NM_022193.1 Latest version!	Rattus norvegicus acetyl-CoA carboxylase alpha (Acaca), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acaca ( NM_022193.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_071529.1 Rattus norvegicus acetyl-CoA carboxylase alpha (Acaca), mRNA.

Price & Availability↑

CloneID	ORa05402
Clone ID Related Accession (Same CDS sequence)	NM_022193.1
Accession Version	NM_022193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	acetyl-CoA carboxylase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J03808.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF121986.1, EF121987.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGATGAAC CATCTCCGTT GGCCAAAACC CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGGTCCG TGTCTGAAGA CAACTCAGAA GATGAGATCA GTAACCTGGT AAAGCTGGAC 
CTAGAGGAGA AGGAGGGCTC CCTGTCACCA GCCTCTGTCA GCTCAGATAC ACTTTCTGAT 
TTGGGAATCT CTGCCTTACA GGATGGTTTG GCCTTTCACA TGAGGTCCAG CATGTCCGGC 
TTGCACCTAG TAAAACAAGG TCGAGACAGA AAGAAAATAG ACTCTCAACG AGATTTCACT 
GTGGCTTCGC CAGCAGAATT TGTTACTCGT TTTGGGGGAA ATAAAGTGAT TGAGAAGGTT 
CTTATCGCCA ACAATGGTAT TGCAGCAGTG AAATGCATGC GATCTATCCG GCGGTGGTCT 
TATGAAATGT TCCGCAATGA ACGTGCCATC CGGTTTGTTG TCATGGTTAC ACCCGAAGAC 
CTTAAAGCCA ATGCAGAATA CATTAAGATG GCGGATCACT ATGTTCCAGT GCCTGGAGGA 
GCAAACAACA ACAATTATGC AAATGTGGAA TTGATTCTTG ATATTGCGAA AAGGATACCT 
GTACAGGCAG TGTGGGCTGG CTGGGGTCAT GCCTCCGAGA ACCCCAAGCT CCCGGAGCTA 
CTCTTAAAAA ATGGCATTGC TTTCATGGGC CCTCCGAGTC AGGCCATGTG GGCGTTGGGG 
GATAAGATTG CATCGTCTAT TGTGGCTCAA ACTGCAGGTA TCCCCACTCT TCCCTGGAGT 
GGCAGTGGTC TTCGAGTGGA TTGGCAAGAA AATGATTTTT CGAAACGCAT CTTAAATGTT 
CCACAGGATC TGTATGAGAA AGGCTATGTG AAGGATGTGG ATGATGGACT GAAGGCAGCC 
GAGGAGGTTG GCTATCCAGT GATGATCAAG GCCTCAGAGG GAGGAGGAGG GAAGGGAATC 
AGAAAAGTTA ACAATGCAGA TGACTTCCCT AACCTCTTCA GACAGGTTCA AGCTGAAGTC 
CCTGGATCAC CTATATTTGT AATGAGATTA GCAAAACAGT CTCGTCATCT GGAGGTCCAG 
ATTCTGGCGG ATCAGTATGG CAATGCAATT TCTTTGTTTG GTCGTGACTG CTCTGTACAA 
CGCAGGCATC AGAAGATCAT TGAAGAAGCT CCTGCTGCTA TTGCTACCCC AGCAGTATTT 
GAACACATGG AACAGTGTGC TGTAAAACTT GCCAAAATGG TTGGTTATGT GAGCGCTGGG 
ACTGTGGAAT ACTTGTACAG CCAGGACGGA AGCTTCTACT TCTTGGAACT GAACCCTCGG 
CTACAGGTTG AACATCCTTG TACAGAGATG GTGGCTGATG TCAATCTTCC TGCAGCACAG 
CTCCAGATTG CCATGGGGAT CCCTCTATTT AGGATCAAGG ATATTCGTAT GATGTATGGG 
GTATCTCCTT GGGGTGATGC TCCCATTGAT TTTGAAAATT CTGCTCATGT TCCTTGCCCA 
AGGGGCCATG TGATTGCTGC TCGGATCACC AGTGAAAACC CAGATGAGGG GTTTAAGCCC 
AGCTCTGGAA CAGTTCAGGA ACTTAATTTT CGCAGCAATA AGAATGTTTG GGGTTATTTC 
AGTGTTGCTG CTGCTGGGGG ACTTCATGAA TTTGCTGATT CTCAGTTCGG TCACTGCTTT 
TCCTGGGGAG AAAACAGAGA AGAAGCAATT TCAAATATGG TGGTGGCATT GAAGGAGCTG 
TCTATTCGGG GTGACTTTCG AACTACAGTA GAATACCTCA TCAAGCTGCT GGAGACAGAA 
AGCTTTCAGT TGAACAGAAT CGACACTGGC TGGCTGGACA GACTGATTGC AGAGAAAGTG 
CAGGCAGAGC GTCCTGACAC CATGTTGGGA GTTGTGTGTG GGGCTCTTCA TGTGGCAGAT 
GTGAACCTGA GAAATAGCAT CTCTAACTTC CTTCACTCCT TAGAGAGGGG TCAAGTCCTT 
CCTGCTCACA CACTTCTGAA CACAGTAGAT GTTGAACTTA TCTATGAAGG AATCAAATAT 
GTACTTAAGG TGACTCGGCA GTCTCCCAAC TCCTACGTGG TGATAATGAA CGGCTCGTGT 
GTGGAAGTGG ACGTGCACCG GCTGAGTGAT GGTGGACTGC TCTTGTCCTA CGATGGCAGC 
AGTTACACCA CATACATGAA GGAGGAGGTA GACAGATACC GAATCACAAT TGGCAATAAA 
ACCTGTGTGT TTGAGAAGGA AAATGACCCG TCTGTAATGC GCTCCCCGTC TGCTGGGAAG 
TTAATCCAGT ATATTGTGGA AGATGGAGGC CATGTGTTTG CTGGCCAGTG CTATGCAGAG 
ATTGAGGTAA TGAAGATGGT AATGACTTTG ACAGCTGTAG AATCTGGCTG CATCCATTAT 
GTCAAGCGAC CTGGAGCAGC ACTTGACCCA GGCTGTGTGA TAGCCAAAAT GCAGCTGGAC 
AATCCCAGTA AAGTTCAACA GGCTGAGCTT CACACGGGCA GTCTGCCCCA GATCCAGAGC 
ACAGCTCTCC GAGGCGAAAA GCTCCATCGA GTTTTCCACT ATGTCCTGGA TAACCTGGTC 
AATGTGATGA ATGGATACTG CCTTCCAGAC CCTTTCTTCA GCAGCAAGGT AAAGGACTGG 
GTAGAACGGT TAATGAAGAC TCTGAGAGAC CCCTCCCTGC CTCTTCTAGA ATTGCAGGAT 
ATCATGACCA GTGTCTCTGG CCGGATCCCC CTCAACGTGG AGAAGTCTAT TAAGAAGGAA 
ATGGCTCAGT ATGCTAGCAA CATCACATCG GTCCTGTGTC AGTTTCCCAG CCAGCAGATT 
GCCAACATCC TAGATAGTCA TGCAGCTACA CTGAACCGGA AATCGGAGCG GGAAGTCTTC 
TTCATGAACA CCCAGAGCAT TGTCCAGCTG GTGCAGAGGT ACCGAAGTGG CATCCGTGGC 
CACATGAAGG CTGTGGTGAT GGATCTGCTC CGGCAGTACC TGCGGGTGGA GACACAGTTT 
CAGAATGGCC ACTACGACAA ATGTGTATTC GCCCTTCGGG AAGAGAACAA AAGTGACATG 
AACACCGTAC TGAACTACAT CTTCTCCCAC GCCCAGGTCA CCAAGAAGAA TCTCCTGGTG 
ACAATGCTTA TTGATCAGTT GTGTGGCCGG GACCCTACAC TTACTGATGA GCTGCTAAAC 
ATCCTCACAG AGCTAACTCA GCTCAGCAAA ACCACCAACG CCAAAGTGGC ACTGCGGGCT 
CGCCAGGTTC TTATTGCCTC CCATTTGCCA TCGTACGACG TTCGCCATAA CCAAGTAGAG 
TCCATCTTCT TATCAGCCAT CGACATGTAT GGACACCAGT TTTGCATTGA GAACCTGCAG 
AAACTCATCC TATCAGAAAC ATCTATTTTC GATGTCCTCC CAAACTTTTT TTACCACAGC 
AACCAGGTGG TGAGGATGGC GGCTCTGGAG GTATATGTTC GAAGAGCTTA TATCGCCTAT 
GAGCTCAACA GTGTACAGCA TCGCCAGCTT AAGGACAACA CCTGTGTGGT AGAATTTCAG 
TTCATGCTGC CCACATCTCA TCCAAACAGA GGGAACATCC CCACGCTAAA CAGAATGTCC 
TTTGCCTCCA ACCTCAACCA CTACGGCATG ACTCATGTAG CTAGTGTCAG CGATGTTCTG 
TTGGACAACG CCTTCACACC ACCTTGTCAA CGCATGGGCG GGATGGTCTC TTTCCGGACC 
TTTGAAGATT TCGTCAGGAT CTTTGATGAA GTAATGGGCT GCTTCTGCGA CTCCCCACCC 
CAAAGCCCCA CATTCCCAGA GTCCGGTCAC ACTTCACTCT ATGATGAGGA CAAGGTCCCC 
AGGGACGAAC CAATACATAT TCTGAATGTG GCTATCAAGA CTGATGGCGA TATTGAGGAT 
GACAGGCTTG CAGCTATGTT CAGAGAGTTC ACCCAACAGA ATAAAGCTAC TCTGGTTGAG 
CATGGGATCC GGCGACTTAC GTTCCTAGTT GCACAAAAGG ATTTCAGAAA ACAAGTCAAC 
TGTGAGGTGG ATCAGAGATT TCATAGAGAA TTCCCCAAAT TTTTCACATT CCGAGCAAGG 
GATAAGTTTG AGGAGGACCG CATTTATCGA CATCTGGAGC CTGCTCTGGC TTTCCAGTTA 
GAGCTGAACC GGATGAGAAA TTTTGACCTT ACTGCCATTC CATGCGCTAA TCACAAGATG 
CACCTGTACC TTGGGGCTGC TAAGGTGGAA GTAGGCACAG AAGTGACTGA CTACAGGTTC 
TTTGTTCGTG CGATCATCAG GCACTCTGAT CTGGTCACGA AGGAAGCTTC TTTTGAATAT 
CTACAAAATG AAGGAGAGCG ACTGCTCCTG GAAGCCATGG ATGAATTGGA AGTTGCTTTC 
AATAACACAA ATGTTCGCAC AGACTGTAAC CATATATTCC TCAACTTTGT GCCCACAGTC 
ATCATGGACC CATCAAAGAT TGAAGAATCT GTGCGGAGCA TGGTAATGCG CTATGGAAGC 
CGGCTGTGGA AATTGCGGGT CCTCCAGGCA GAACTGAAAA TCAACATTCG CCTGACAACA 
ACTGGAAAAG CGATTCCCAT CCGCCTCTTC CTGACAAACG AGTCTGGCTA CTACTTGGAC 
ATCAGCCTGT ATAAGGAAGT GACTGACTCC AGGACAGCAC AGATCATGTT TCAGGCGTAT 
GGAGACAAGC AGGGACCACT GCATGGAATG TTAATCAATA CTCCGTATGT GACCAAAGAC 
CTTCTTCAAT CAAAGAGGTT TCAGGCACAG TCCTTGGGAA CAACGTATAT ATATGATATC 
CCAGAGATGT TTCGGCAGTC GCTCATCAAG CTCTGGGAGT CCATGTCCAC TCAAGCATTT 
CTTCCTTCAC CCCCTTTGCC TTCCGACATA CTGACGTATA CTGAACTGGT GTTGGATGAT 
CAAGGCCAGC TTGTCCATAT GAACAGACTT CCAGGAGGAA ACGAGATTGG CATGGTAGCC 
TGGAAAATGA GCCTTAAAAG CCCTGAATAT CCAGATGGCC GAGATGTCAT TGTCATCGGC 
AATGACATTA CATATCGGAT TGGCTCCTTT GGGCCTCAGG AAGATTTGCT GTTTCTCAGA 
GCTTCTGAAC TTGCCAGAGC AGAAGGCATC CCACGCATCT ACGTAGCAGC CAACAGTGGA 
GCTAGAATTG GACTGGCAGA AGAAATCCGT CATATGTTCC ACGTGGCCTG GGTAGACTCT 
GAGGATCCTT ACAAGGGATA CAAGTATTTA TATCTGACAC CCCAGGATTA TAAAAGAGTG 
AGTGCTCTCA ATTCTGTCCA CTGTGAACAT GTGGAAGATG AAGGAGAATC CAGGTACAAG 
ATAACAGACA TTATCGGGAA AGAAGAAGGA CTTGGAGCAG AGAACCTTCG GGGTTCTGGA 
ATGATTGCTG GGGAATCCTC ATTGGCTTAT GATGAGATCA TCACCATCAG CCTGGTTACA 
TGCCGGGCCA TTGGTATTGG GGCTTACCTT GTCCGGCTGG GACAAAGAAC CATCCAGGTT 
GAGAACTCTC ACTTAATTCT AACAGGAGCC GGTGCCCTCA ACAAAGTCCT TGGTCGGGAA 
GTATACACCT CCAACAATCA GCTTGGGGGC ATCCAGATAA TGCACAACAA CGGAGTTACC 
CATTGCACTG TTTGTGATGA CTTTGAGGGA GTGTTCACAG TCTTACACTG GCTGTCATAC 
ATGCCTAAGA ACGTGCACAG TTCAGTTCCT CTCCTGAATT CCAAGGATCC TATAGATAGA 
ATCATCGAGT TTGTTCCCAC AAAGGCCCCG TATGATCCTC GGTGGATGCT GGCAGGCCGT 
CCTCACCCAA CCCAGAAAGG CCAATGGTTG AGTGGATTTT TTGATTATGG CTCTTTCTCA 
GAAATCATGC AGCCCTGGGC GCAGACCGTG GTAGTTGGCA GAGCCAGGTT GGGGGGAATA 
CCTGTGGGAG TAGTTGCTGT AGAAACCCGA ACCGTGGAGC TCAGTGTACC AGCTGATCCT 
GCAAACCTGG ATTCTGAAGC CAAGATAATC CAGCAGGCCG GCCAAGTTTG GTTTCCAGAC 
TCTGCATTTA AGACCTATCA AGCTATCAAG GACTTTAACC GTGAAGGGCT ACCTCTAATG 
GTCTTTGCCA ACTGGAGAGG CTTCTCTGGT GGGATGAAAG ATATGTATGA CCAGGTGCTC 
AAGTTTGGTG CTTATATTGT GGATGGCTTG CGGGAATGTT CCCAGCCTGT GATGGTCTAC 
ATTCCCCCAC AGGCTGAGCT TCGGGGTGGT TCTTGGGTTG TGATCGACCC AACCATCAAT 
CCTCGGCACA TGGAGATGTA TGCTGACCGG GAAAGCAGGG GATCCGTTCT GGAACCAGAA 
GGGACAGTAG AAATCAAATT CCGCAAAAAG GATCTGGTGA AAACCATGCG TCGCGTAGAC 
CCAGTCTACA TCCGCTTGGC TGAGCGACTG GGCACCCCAG AGCTAAGCCC CACTGAGCGG 
AAGGAGCTGG AGAGCAAGTT GAAGGAGCGG GAGGAGTTCC TAATTCCCAT TTACCATCAG 
GTAGCTGTGC AGTTTGCTGA CTTGCACGAC ACCCCAGGCC GGATGCAGGA GAAGGGTGTC 
ATTAATGATA TCTTAGATTG GAAAACATCC CGCACCTTCT TCTACTGGCG ACTGAGGCGT 
CTCCTGCTGG AAGACCTGGT CAAGAAGAAA ATCCACAGTG CCAACCCTGA GCTGACCGAT 
GGCCAGATCC AGGCCATGTT GAGACGCTGG TTTGTGGAAG TGGAAGGCAC AGTGAAGGCT 
TACGTCTGGG ACAATAATAA GGATCTGGTG GAATGGCTGG AGAAGCAGCT GACAGAGGAA 
GATGGTGTCC GCTCTGTGAT AGAGGAGAAC ATCAAATACA TCAGCAGGGA CTATGTCCTC 
AAGCAGATCC GCAGCTTGGT GCAGGCCAAT CCAGAAGTTG CCATGGACTC CATCGTCCAC 
ATGACCCAGC ACATCTCCCC CACTCAGCGA GCAGAGGTTG TAAGGATCCT TTCCACTATG 
GACTCCCCTT CTACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071529.1
CDS	1..7038
Translation	MDEPSPLAKTLELNQHSRFIIGSVSEDNSEDEISNLVKLDLEEKEGSLSPASVSSDTLSDLGISALQDGLAFHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGANNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWQENDFSKRILNVPQDLYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLFRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVNLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYEGIKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPQIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPLNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYDVRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFASNLNHYGMTHVASVSDVLLDNAFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPESGHTSLYDEDKVPRDEPIHILNVAIKTDGDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTTTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDILTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMSLKSPEYPDGRDVIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIYVAANSGARIGLAEEIRHMFHVAWVDSEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHCTVCDDFEGVFTVLHWLSYMPKNVHSSVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSVPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDGLRECSQPVMVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRKKDLVKTMRRVDPVYIRLAERLGTPELSPTERKELESKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHSANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	NM_022193.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acetyl-CoA carboxylase alpha (Acaca), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022193.1

ORF Insert Sequence:

ATGGATGAAC CATCTCCGTT GGCCAAAACC CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGGTCCG TGTCTGAAGA CAACTCAGAA GATGAGATCA GTAACCTGGT AAAGCTGGAC 
CTAGAGGAGA AGGAGGGCTC CCTGTCACCA GCCTCTGTCA GCTCAGATAC ACTTTCTGAT 
TTGGGAATCT CTGCCTTACA GGATGGTTTG GCCTTTCACA TGAGGTCCAG CATGTCCGGC 
TTGCACCTAG TAAAACAAGG TCGAGACAGA AAGAAAATAG ACTCTCAACG AGATTTCACT 
GTGGCTTCGC CAGCAGAATT TGTTACTCGT TTTGGGGGAA ATAAAGTGAT TGAGAAGGTT 
CTTATCGCCA ACAATGGTAT TGCAGCAGTG AAATGCATGC GATCTATCCG GCGGTGGTCT 
TATGAAATGT TCCGCAATGA ACGTGCCATC CGGTTTGTTG TCATGGTTAC ACCCGAAGAC 
CTTAAAGCCA ATGCAGAATA CATTAAGATG GCGGATCACT ATGTTCCAGT GCCTGGAGGA 
GCAAACAACA ACAATTATGC AAATGTGGAA TTGATTCTTG ATATTGCGAA AAGGATACCT 
GTACAGGCAG TGTGGGCTGG CTGGGGTCAT GCCTCCGAGA ACCCCAAGCT CCCGGAGCTA 
CTCTTAAAAA ATGGCATTGC TTTCATGGGC CCTCCGAGTC AGGCCATGTG GGCGTTGGGG 
GATAAGATTG CATCGTCTAT TGTGGCTCAA ACTGCAGGTA TCCCCACTCT TCCCTGGAGT 
GGCAGTGGTC TTCGAGTGGA TTGGCAAGAA AATGATTTTT CGAAACGCAT CTTAAATGTT 
CCACAGGATC TGTATGAGAA AGGCTATGTG AAGGATGTGG ATGATGGACT GAAGGCAGCC 
GAGGAGGTTG GCTATCCAGT GATGATCAAG GCCTCAGAGG GAGGAGGAGG GAAGGGAATC 
AGAAAAGTTA ACAATGCAGA TGACTTCCCT AACCTCTTCA GACAGGTTCA AGCTGAAGTC 
CCTGGATCAC CTATATTTGT AATGAGATTA GCAAAACAGT CTCGTCATCT GGAGGTCCAG 
ATTCTGGCGG ATCAGTATGG CAATGCAATT TCTTTGTTTG GTCGTGACTG CTCTGTACAA 
CGCAGGCATC AGAAGATCAT TGAAGAAGCT CCTGCTGCTA TTGCTACCCC AGCAGTATTT 
GAACACATGG AACAGTGTGC TGTAAAACTT GCCAAAATGG TTGGTTATGT GAGCGCTGGG 
ACTGTGGAAT ACTTGTACAG CCAGGACGGA AGCTTCTACT TCTTGGAACT GAACCCTCGG 
CTACAGGTTG AACATCCTTG TACAGAGATG GTGGCTGATG TCAATCTTCC TGCAGCACAG 
CTCCAGATTG CCATGGGGAT CCCTCTATTT AGGATCAAGG ATATTCGTAT GATGTATGGG 
GTATCTCCTT GGGGTGATGC TCCCATTGAT TTTGAAAATT CTGCTCATGT TCCTTGCCCA 
AGGGGCCATG TGATTGCTGC TCGGATCACC AGTGAAAACC CAGATGAGGG GTTTAAGCCC 
AGCTCTGGAA CAGTTCAGGA ACTTAATTTT CGCAGCAATA AGAATGTTTG GGGTTATTTC 
AGTGTTGCTG CTGCTGGGGG ACTTCATGAA TTTGCTGATT CTCAGTTCGG TCACTGCTTT 
TCCTGGGGAG AAAACAGAGA AGAAGCAATT TCAAATATGG TGGTGGCATT GAAGGAGCTG 
TCTATTCGGG GTGACTTTCG AACTACAGTA GAATACCTCA TCAAGCTGCT GGAGACAGAA 
AGCTTTCAGT TGAACAGAAT CGACACTGGC TGGCTGGACA GACTGATTGC AGAGAAAGTG 
CAGGCAGAGC GTCCTGACAC CATGTTGGGA GTTGTGTGTG GGGCTCTTCA TGTGGCAGAT 
GTGAACCTGA GAAATAGCAT CTCTAACTTC CTTCACTCCT TAGAGAGGGG TCAAGTCCTT 
CCTGCTCACA CACTTCTGAA CACAGTAGAT GTTGAACTTA TCTATGAAGG AATCAAATAT 
GTACTTAAGG TGACTCGGCA GTCTCCCAAC TCCTACGTGG TGATAATGAA CGGCTCGTGT 
GTGGAAGTGG ACGTGCACCG GCTGAGTGAT GGTGGACTGC TCTTGTCCTA CGATGGCAGC 
AGTTACACCA CATACATGAA GGAGGAGGTA GACAGATACC GAATCACAAT TGGCAATAAA 
ACCTGTGTGT TTGAGAAGGA AAATGACCCG TCTGTAATGC GCTCCCCGTC TGCTGGGAAG 
TTAATCCAGT ATATTGTGGA AGATGGAGGC CATGTGTTTG CTGGCCAGTG CTATGCAGAG 
ATTGAGGTAA TGAAGATGGT AATGACTTTG ACAGCTGTAG AATCTGGCTG CATCCATTAT 
GTCAAGCGAC CTGGAGCAGC ACTTGACCCA GGCTGTGTGA TAGCCAAAAT GCAGCTGGAC 
AATCCCAGTA AAGTTCAACA GGCTGAGCTT CACACGGGCA GTCTGCCCCA GATCCAGAGC 
ACAGCTCTCC GAGGCGAAAA GCTCCATCGA GTTTTCCACT ATGTCCTGGA TAACCTGGTC 
AATGTGATGA ATGGATACTG CCTTCCAGAC CCTTTCTTCA GCAGCAAGGT AAAGGACTGG 
GTAGAACGGT TAATGAAGAC TCTGAGAGAC CCCTCCCTGC CTCTTCTAGA ATTGCAGGAT 
ATCATGACCA GTGTCTCTGG CCGGATCCCC CTCAACGTGG AGAAGTCTAT TAAGAAGGAA 
ATGGCTCAGT ATGCTAGCAA CATCACATCG GTCCTGTGTC AGTTTCCCAG CCAGCAGATT 
GCCAACATCC TAGATAGTCA TGCAGCTACA CTGAACCGGA AATCGGAGCG GGAAGTCTTC 
TTCATGAACA CCCAGAGCAT TGTCCAGCTG GTGCAGAGGT ACCGAAGTGG CATCCGTGGC 
CACATGAAGG CTGTGGTGAT GGATCTGCTC CGGCAGTACC TGCGGGTGGA GACACAGTTT 
CAGAATGGCC ACTACGACAA ATGTGTATTC GCCCTTCGGG AAGAGAACAA AAGTGACATG 
AACACCGTAC TGAACTACAT CTTCTCCCAC GCCCAGGTCA CCAAGAAGAA TCTCCTGGTG 
ACAATGCTTA TTGATCAGTT GTGTGGCCGG GACCCTACAC TTACTGATGA GCTGCTAAAC 
ATCCTCACAG AGCTAACTCA GCTCAGCAAA ACCACCAACG CCAAAGTGGC ACTGCGGGCT 
CGCCAGGTTC TTATTGCCTC CCATTTGCCA TCGTACGACG TTCGCCATAA CCAAGTAGAG 
TCCATCTTCT TATCAGCCAT CGACATGTAT GGACACCAGT TTTGCATTGA GAACCTGCAG 
AAACTCATCC TATCAGAAAC ATCTATTTTC GATGTCCTCC CAAACTTTTT TTACCACAGC 
AACCAGGTGG TGAGGATGGC GGCTCTGGAG GTATATGTTC GAAGAGCTTA TATCGCCTAT 
GAGCTCAACA GTGTACAGCA TCGCCAGCTT AAGGACAACA CCTGTGTGGT AGAATTTCAG 
TTCATGCTGC CCACATCTCA TCCAAACAGA GGGAACATCC CCACGCTAAA CAGAATGTCC 
TTTGCCTCCA ACCTCAACCA CTACGGCATG ACTCATGTAG CTAGTGTCAG CGATGTTCTG 
TTGGACAACG CCTTCACACC ACCTTGTCAA CGCATGGGCG GGATGGTCTC TTTCCGGACC 
TTTGAAGATT TCGTCAGGAT CTTTGATGAA GTAATGGGCT GCTTCTGCGA CTCCCCACCC 
CAAAGCCCCA CATTCCCAGA GTCCGGTCAC ACTTCACTCT ATGATGAGGA CAAGGTCCCC 
AGGGACGAAC CAATACATAT TCTGAATGTG GCTATCAAGA CTGATGGCGA TATTGAGGAT 
GACAGGCTTG CAGCTATGTT CAGAGAGTTC ACCCAACAGA ATAAAGCTAC TCTGGTTGAG 
CATGGGATCC GGCGACTTAC GTTCCTAGTT GCACAAAAGG ATTTCAGAAA ACAAGTCAAC 
TGTGAGGTGG ATCAGAGATT TCATAGAGAA TTCCCCAAAT TTTTCACATT CCGAGCAAGG 
GATAAGTTTG AGGAGGACCG CATTTATCGA CATCTGGAGC CTGCTCTGGC TTTCCAGTTA 
GAGCTGAACC GGATGAGAAA TTTTGACCTT ACTGCCATTC CATGCGCTAA TCACAAGATG 
CACCTGTACC TTGGGGCTGC TAAGGTGGAA GTAGGCACAG AAGTGACTGA CTACAGGTTC 
TTTGTTCGTG CGATCATCAG GCACTCTGAT CTGGTCACGA AGGAAGCTTC TTTTGAATAT 
CTACAAAATG AAGGAGAGCG ACTGCTCCTG GAAGCCATGG ATGAATTGGA AGTTGCTTTC 
AATAACACAA ATGTTCGCAC AGACTGTAAC CATATATTCC TCAACTTTGT GCCCACAGTC 
ATCATGGACC CATCAAAGAT TGAAGAATCT GTGCGGAGCA TGGTAATGCG CTATGGAAGC 
CGGCTGTGGA AATTGCGGGT CCTCCAGGCA GAACTGAAAA TCAACATTCG CCTGACAACA 
ACTGGAAAAG CGATTCCCAT CCGCCTCTTC CTGACAAACG AGTCTGGCTA CTACTTGGAC 
ATCAGCCTGT ATAAGGAAGT GACTGACTCC AGGACAGCAC AGATCATGTT TCAGGCGTAT 
GGAGACAAGC AGGGACCACT GCATGGAATG TTAATCAATA CTCCGTATGT GACCAAAGAC 
CTTCTTCAAT CAAAGAGGTT TCAGGCACAG TCCTTGGGAA CAACGTATAT ATATGATATC 
CCAGAGATGT TTCGGCAGTC GCTCATCAAG CTCTGGGAGT CCATGTCCAC TCAAGCATTT 
CTTCCTTCAC CCCCTTTGCC TTCCGACATA CTGACGTATA CTGAACTGGT GTTGGATGAT 
CAAGGCCAGC TTGTCCATAT GAACAGACTT CCAGGAGGAA ACGAGATTGG CATGGTAGCC 
TGGAAAATGA GCCTTAAAAG CCCTGAATAT CCAGATGGCC GAGATGTCAT TGTCATCGGC 
AATGACATTA CATATCGGAT TGGCTCCTTT GGGCCTCAGG AAGATTTGCT GTTTCTCAGA 
GCTTCTGAAC TTGCCAGAGC AGAAGGCATC CCACGCATCT ACGTAGCAGC CAACAGTGGA 
GCTAGAATTG GACTGGCAGA AGAAATCCGT CATATGTTCC ACGTGGCCTG GGTAGACTCT 
GAGGATCCTT ACAAGGGATA CAAGTATTTA TATCTGACAC CCCAGGATTA TAAAAGAGTG 
AGTGCTCTCA ATTCTGTCCA CTGTGAACAT GTGGAAGATG AAGGAGAATC CAGGTACAAG 
ATAACAGACA TTATCGGGAA AGAAGAAGGA CTTGGAGCAG AGAACCTTCG GGGTTCTGGA 
ATGATTGCTG GGGAATCCTC ATTGGCTTAT GATGAGATCA TCACCATCAG CCTGGTTACA 
TGCCGGGCCA TTGGTATTGG GGCTTACCTT GTCCGGCTGG GACAAAGAAC CATCCAGGTT 
GAGAACTCTC ACTTAATTCT AACAGGAGCC GGTGCCCTCA ACAAAGTCCT TGGTCGGGAA 
GTATACACCT CCAACAATCA GCTTGGGGGC ATCCAGATAA TGCACAACAA CGGAGTTACC 
CATTGCACTG TTTGTGATGA CTTTGAGGGA GTGTTCACAG TCTTACACTG GCTGTCATAC 
ATGCCTAAGA ACGTGCACAG TTCAGTTCCT CTCCTGAATT CCAAGGATCC TATAGATAGA 
ATCATCGAGT TTGTTCCCAC AAAGGCCCCG TATGATCCTC GGTGGATGCT GGCAGGCCGT 
CCTCACCCAA CCCAGAAAGG CCAATGGTTG AGTGGATTTT TTGATTATGG CTCTTTCTCA 
GAAATCATGC AGCCCTGGGC GCAGACCGTG GTAGTTGGCA GAGCCAGGTT GGGGGGAATA 
CCTGTGGGAG TAGTTGCTGT AGAAACCCGA ACCGTGGAGC TCAGTGTACC AGCTGATCCT 
GCAAACCTGG ATTCTGAAGC CAAGATAATC CAGCAGGCCG GCCAAGTTTG GTTTCCAGAC 
TCTGCATTTA AGACCTATCA AGCTATCAAG GACTTTAACC GTGAAGGGCT ACCTCTAATG 
GTCTTTGCCA ACTGGAGAGG CTTCTCTGGT GGGATGAAAG ATATGTATGA CCAGGTGCTC 
AAGTTTGGTG CTTATATTGT GGATGGCTTG CGGGAATGTT CCCAGCCTGT GATGGTCTAC 
ATTCCCCCAC AGGCTGAGCT TCGGGGTGGT TCTTGGGTTG TGATCGACCC AACCATCAAT 
CCTCGGCACA TGGAGATGTA TGCTGACCGG GAAAGCAGGG GATCCGTTCT GGAACCAGAA 
GGGACAGTAG AAATCAAATT CCGCAAAAAG GATCTGGTGA AAACCATGCG TCGCGTAGAC 
CCAGTCTACA TCCGCTTGGC TGAGCGACTG GGCACCCCAG AGCTAAGCCC CACTGAGCGG 
AAGGAGCTGG AGAGCAAGTT GAAGGAGCGG GAGGAGTTCC TAATTCCCAT TTACCATCAG 
GTAGCTGTGC AGTTTGCTGA CTTGCACGAC ACCCCAGGCC GGATGCAGGA GAAGGGTGTC 
ATTAATGATA TCTTAGATTG GAAAACATCC CGCACCTTCT TCTACTGGCG ACTGAGGCGT 
CTCCTGCTGG AAGACCTGGT CAAGAAGAAA ATCCACAGTG CCAACCCTGA GCTGACCGAT 
GGCCAGATCC AGGCCATGTT GAGACGCTGG TTTGTGGAAG TGGAAGGCAC AGTGAAGGCT 
TACGTCTGGG ACAATAATAA GGATCTGGTG GAATGGCTGG AGAAGCAGCT GACAGAGGAA 
GATGGTGTCC GCTCTGTGAT AGAGGAGAAC ATCAAATACA TCAGCAGGGA CTATGTCCTC 
AAGCAGATCC GCAGCTTGGT GCAGGCCAAT CCAGAAGTTG CCATGGACTC CATCGTCCAC 
ATGACCCAGC ACATCTCCCC CACTCAGCGA GCAGAGGTTG TAAGGATCCT TTCCACTATG 
GACTCCCCTT CTACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Obesity-alleviating potential of asiatic acid and its effects on ACC1, UCP2, and CPT1 mRNA expression in high fat diet-induced obese Sprague-Dawley rats.
Molecular and cellular biochemistry(2017 Oct)
Rameshreddy P,Uddandrao VVS,Brahmanaidu P,Vadivukkarasi S,Ravindarnaik R,Suresh P,Swapna K,Kalaivani A,Parvathi P,Tamilmani P,Saravanan G

Liver X Receptor Agonist TO901317 Prevents Diacylglycerols Accumulation in the Heart of Streptozotocin-Diabetic Rats.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology39(1)350-9(2016)
Harasiuk D,Baranowski M,Zabielski P,Chabowski A,G?rski J

Selective impairment of P2Y signaling by prostaglandin E2 in macrophages: implications for Ca2+-dependent responses.
Journal of immunology (Baltimore, Md. : 1950)190(8)4226-35(2013 Apr)
Trav?s PG,Pimentel-Santillana M,Carrasquero LM,P?rez-Sen R,Delicado EG,Luque A,Izquierdo M,Mart?n-Sanz P,Miras-Portugal MT,Bosc? L

Changes in white adipose tissue metabolism induced by resveratrol in rats.
Nutrition & metabolism8(1)29(2011 May)
Alberdi G,Rodr?guez VM,Miranda J,Macarulla MT,Arias N,Andr?s-Lacueva C,Portillo MP

Crystal structure of Spot 14, a modulator of fatty acid synthesis.
Proceedings of the National Academy of Sciences of the United States of America107(44)18820-5(2010 Nov)
Colbert CL,Kim CW,Moon YA,Henry L,Palnitkar M,McKean WB,Fitzgerald K,Deisenhofer J,Horton JD,Kwon HJ

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Acaca cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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