ACC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ACC1
Entrez Gene ID	855750
Full Name	acetyl-CoA carboxylase ACC1
Synonyms	ABP2,FAS3,MTR7
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183193.1	NP_014413.1	acetyl-CoA carboxylase ACC1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ACACA	XP_005624832.1
Bos taurus (cattle)	ACACA	NP_776649.1
Mus musculus (house mouse)	Acaca	NP_579938.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005175	XP_314071.4
Schizosaccharomyces pombe (fission yeast)	cut6	NP_593271.1
Arabidopsis thaliana (thale cress)	ACC1	NP_174849.2
	ACACA	XP_001111823.1
Rattus norvegicus (Norway rat)	Acaca	NP_071529.1
Magnaporthe oryzae (rice blast fungus)	MGG_07613	XP_003711534.1
Drosophila melanogaster (fruit fly)	ACC	NP_724636.1
Saccharomyces cerevisiae (baker's yeast)	ACC1	NP_014413.1
Eremothecium gossypii	AGOS_AAR071W	NP_982612.1
Neurospora crassa	NCU08535	XP_963017.1
Pan troglodytes (chimpanzee)	ACACA	XP_511428.3
Gallus gallus (chicken)	ACACA	NP_990836.1
Arabidopsis thaliana (thale cress)	ACC2	NP_174850.4
Homo sapiens (human)	ACACA	NP_942131.1
Danio rerio (zebrafish)	acaca	NP_001258237.1
Kluyveromyces lactis	KLLA0F06072g	XP_455355.1
Xenopus tropicalis (tropical clawed frog)	acaca	XP_004911956.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

urther our work suggests that NuA4 regulates acetyl-CoA levels through the Acetyl-CoA carboxylase Acc1. Altogether this work establishes both NuA4 and the metabolite acetyl-CoA as critical signaling pathways regulating the formation of glucose-deprived stress granules.
Title: Lysine acetyltransferase NuA4 and acetyl-CoA regulate glucose-deprived stress granule formation in Saccharomyces cerevisiae.

Overexpression of FAS1, FAS2 and Acc1 leads to accumulation of sterols.
Title: Overexpression of genes of the fatty acid biosynthetic pathway leads to accumulation of sterols in Saccharomyces cerevisiae.

Data show that attenuated expression of acetyl-CoA carboxylase ACC1 results in increased acetylation of bulk histones, globally increased acetylation of chromatin histones, and altered transcriptional regulation.
Title: Acetyl-CoA carboxylase regulates global histone acetylation.

The following ACC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04640	NM_001183193.1 Latest version!	Saccharomyces cerevisiae S288C acetyl-CoA carboxylase ACC1 (ACC1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACC1 ( NM_001183193.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014413.1 Saccharomyces cerevisiae S288C acetyl-CoA carboxylase ACC1 (ACC1), partial mRNA.

Price & Availability↑

CloneID	OSi04640
Clone ID Related Accession (Same CDS sequence)	NM_001183193.1
Accession Version	NM_001183193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	acetyl-CoA carboxylase ACC1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGCGAAG AAAGCTTATT CGAGTCTTCT CCACAGAAGA TGGAGTACGA AATTACAAAC 
TACTCAGAAA GACATACAGA ACTTCCAGGT CATTTCATTG GCCTCAATAC AGTAGATAAA 
CTAGAGGAGT CCCCGTTAAG GGACTTTGTT AAGAGTCACG GTGGTCACAC GGTCATATCC 
AAGATCCTGA TAGCAAATAA TGGTATTGCC GCCGTGAAAG AAATTAGATC CGTCAGAAAA 
TGGGCATACG AGACGTTCGG CGATGACAGA ACCGTCCAAT TCGTCGCCAT GGCCACCCCA 
GAAGATCTGG AGGCCAACGC AGAATATATC CGTATGGCCG ATCAATACAT TGAAGTGCCA 
GGTGGTACTA ATAATAACAA CTACGCTAAC GTAGACTTGA TCGTAGACAT CGCCGAAAGA 
GCAGACGTAG ACGCCGTATG GGCTGGCTGG GGTCACGCCT CCGAGAATCC ACTATTGCCT 
GAAAAATTGT CCCAGTCTAA GAGGAAAGTC ATCTTTATTG GGCCTCCAGG TAACGCCATG 
AGGTCTTTAG GTGATAAAAT CTCCTCTACC ATTGTCGCTC AAAGTGCTAA AGTCCCATGT 
ATTCCATGGT CTGGTACCGG TGTTGACACC GTTCACGTGG ACGAGAAAAC CGGTCTGGTC 
TCTGTCGACG ATGACATCTA TCAAAAGGGT TGTTGTACCT CTCCTGAAGA TGGTTTACAA 
AAGGCCAAGC GTATTGGTTT TCCTGTCATG ATTAAGGCAT CCGAAGGTGG TGGTGGTAAA 
GGTATCAGAC AAGTTGAACG TGAAGAAGAT TTCATCGCTT TATACCACCA GGCAGCCAAC 
GAAATTCCAG GCTCCCCCAT TTTCATCATG AAGTTGGCCG GTAGAGCGCG TCACTTGGAA 
GTTCAACTGC TAGCAGATCA GTACGGTACA AATATTTCCT TGTTCGGTAG AGACTGTTCC 
GTTCAGAGAC GTCATCAAAA AATTATCGAA GAAGCACCAG TTACAATTGC CAAGGCTGAA 
ACATTTCACG AGATGGAAAA GGCTGCCGTC AGACTGGGGA AACTAGTCGG TTATGTCTCT 
GCCGGTACCG TGGAGTATCT ATATTCTCAT GATGATGGAA AATTCTACTT TTTAGAATTG 
AACCCAAGAT TACAAGTCGA GCATCCAACA ACGGAAATGG TCTCCGGTGT TAACTTACCT 
GCAGCTCAAT TACAAATCGC TATGGGTATC CCTATGCATA GAATAAGTGA CATTAGAACT 
TTATATGGTA TGAATCCTCA TTCTGCCTCA GAAATCGATT TCGAATTCAA AACTCAAGAT 
GCCACCAAGA AACAAAGAAG ACCTATTCCA AAGGGTCATT GTACCGCTTG TCGTATCACA 
TCAGAAGATC CAAACGATGG ATTCAAGCCA TCGGGTGGTA CTTTGCATGA ACTAAACTTC 
CGTTCTTCCT CTAATGTTTG GGGTTACTTC TCCGTGGGTA ACAATGGTAA TATTCACTCC 
TTTTCGGACT CTCAGTTCGG CCATATTTTT GCTTTTGGTG AAAATAGACA AGCTTCCAGG 
AAACACATGG TTGTTGCCCT GAAGGAATTG TCCATTAGGG GTGATTTCAG AACTACTGTG 
GAATACTTGA TCAAACTTTT GGAAACTGAA GATTTCGAGG ATAACACTAT TACCACCGGT 
TGGTTGGACG ATTTGATTAC TCATAAAATG ACCGCTGAAA AGCCTGATCC AACTCTTGCC 
GTCATTTGCG GTGCCGCTAC AAAGGCTTTC TTAGCATCTG AAGAAGCCCG CCACAAGTAT 
ATCGAATCCT TACAAAAGGG ACAAGTTCTA TCTAAAGACC TACTGCAAAC TATGTTCCCT 
GTAGATTTTA TCCATGAGGG TAAAAGATAC AAGTTCACCG TAGCTAAATC CGGTAATGAC 
CGTTACACAT TATTTATCAA TGGTTCTAAA TGTGATATCA TACTGCGTCA ACTATCTGAT 
GGTGGTCTTT TGATTGCCAT AGGCGGTAAA TCGCATACCA TCTATTGGAA AGAAGAAGTT 
GCTGCTACAA GATTATCCGT TGACTCTATG ACTACTTTGT TGGAAGTTGA AAACGATCCA 
ACCCAGTTGC GTACTCCATC CCCTGGTAAA TTGGTTAAAT TCTTGGTGGA AAATGGTGAA 
CACATTATCA AGGGCCAACC ATATGCAGAA ATTGAAGTTA TGAAAATGCA AATGCCTTTG 
GTTTCTCAAG AAAATGGTAT CGTCCAGTTA TTAAAGCAAC CTGGTTCTAC CATTGTTGCA 
GGTGATATCA TGGCTATTAT GACTCTTGAC GATCCATCCA AGGTCAAGCA CGCTCTACCA 
TTTGAAGGTA TGCTGCCAGA TTTTGGTTCT CCAGTTATCG AAGGAACCAA ACCTGCCTAT 
AAATTCAAGT CATTAGTGTC TACTTTGGAA AACATTTTGA AGGGTTATGA CAACCAAGTT 
ATTATGAACG CTTCCTTGCA ACAATTGATA GAGGTTTTGA GAAATCCAAA ACTGCCTTAC 
TCAGAATGGA AACTACACAT CTCTGCTTTA CATTCAAGAT TGCCTGCTAA GCTAGATGAA 
CAAATGGAAG AGTTAGTTGC ACGTTCTTTG AGACGTGGTG CTGTTTTCCC AGCTAGACAA 
TTAAGTAAAT TGATTGATAT GGCCGTGAAG AATCCTGAAT ACAACCCCGA CAAATTGCTG 
GGCGCCGTCG TGGAACCATT GGCGGATATT GCTCATAAGT ACTCTAACGG GTTAGAAGCC 
CATGAACATT CTATATTTGT CCATTTCTTG GAAGAATATT ACGAAGTTGA AAAGTTATTC 
AATGGTCCAA ATGTTCGTGA GGAAAATATC ATTCTGAAAT TGCGTGATGA AAACCCTAAA 
GATCTAGATA AAGTTGCGCT AACTGTTTTG TCTCATTCGA AAGTTTCAGC GAAGAATAAC 
CTGATCCTAG CTATCTTGAA ACATTATCAA CCATTGTGCA AGTTATCTTC TAAAGTTTCT 
GCCATTTTCT CTACTCCTCT ACAACATATT GTTGAACTAG AATCTAAGGC TACCGCTAAG 
GTCGCTCTAC AAGCAAGAGA AATTTTGATT CAAGGCGCTT TACCTTCGGT CAAGGAAAGA 
ACTGAACAAA TTGAACATAT CTTAAAATCC TCTGTTGTGA AGGTTGCCTA TGGCTCATCC 
AATCCAAAGC GCTCTGAACC AGATTTGAAT ATCTTGAAGG ACTTGATCGA TTCTAATTAC 
GTTGTGTTCG ATGTTTTACT TCAATTCCTA ACCCATCAAG ACCCAGTTGT GACTGCTGCA 
GCTGCTCAAG TCTATATTCG TCGTGCTTAT CGTGCTTACA CCATAGGAGA TATTAGAGTT 
CACGAAGGTG TCACAGTTCC AATTGTTGAA TGGAAATTCC AACTACCTTC AGCTGCGTTC 
TCCACCTTTC CAACTGTTAA ATCTAAAATG GGTATGAACA GGGCTGTTTC TGTTTCAGAT 
TTGTCATATG TTGCAAACAG TCAGTCATCT CCGTTAAGAG AAGGTATTTT GATGGCTGTG 
GATCATTTAG ATGATGTTGA TGAAATTTTG TCACAAAGTT TGGAAGTTAT TCCTCGTCAC 
CAATCTTCTT CTAACGGACC TGCTCCTGAT CGTTCTGGTA GCTCCGCATC GTTGAGTAAT 
GTTGCTAATG TTTGTGTTGC TTCTACAGAA GGTTTCGAAT CTGAAGAGGA AATTTTGGTA 
AGGTTGAGAG AAATTTTGGA TTTGAATAAG CAGGAATTAA TCAATGCTTC TATCCGTCGT 
ATCACATTTA TGTTCGGTTT TAAAGATGGG TCTTATCCAA AGTATTATAC TTTTAACGGT 
CCAAATTATA ACGAAAATGA AACAATTCGT CACATTGAGC CGGCTTTGGC CTTCCAACTG 
GAATTAGGAA GATTGTCCAA CTTCAACATT AAACCAATTT TCACTGATAA TAGAAACATC 
CATGTCTACG AAGCTGTTAG TAAGACTTCT CCATTGGATA AGAGATTCTT TACAAGAGGT 
ATTATTAGAA CGGGTCATAT CCGTGATGAC ATTTCTATTC AAGAATATCT GACTTCTGAA 
GCTAACAGAT TGATGAGTGA TATATTGGAT AATTTAGAAG TCACCGACAC TTCAAATTCT 
GATTTGAATC ATATCTTCAT CAACTTCATT GCGGTGTTTG ATATCTCTCC AGAAGATGTC 
GAAGCCGCCT TCGGTGGTTT CTTAGAAAGA TTTGGTAAGA GATTGTTGAG ATTGCGTGTT 
TCTTCTGCCG AAATTAGAAT CATCATCAAA GATCCTCAAA CAGGTGCCCC AGTACCATTG 
CGTGCCTTGA TCAATAACGT TTCTGGTTAT GTTATCAAAA CAGAAATGTA CACCGAAGTC 
AAGAACGCAA AAGGTGAATG GGTATTTAAG TCTTTGGGTA AACCTGGATC CATGCATTTA 
AGACCTATTG CTACTCCTTA CCCTGTTAAG GAATGGTTGC AACCAAAACG TTATAAGGCA 
CACTTGATGG GTACCACATA TGTCTATGAC TTCCCAGAAT TATTCCGCCA AGCATCGTCA 
TCCCAATGGA AAAATTTCTC TGCAGATGTT AAGTTAACAG ATGATTTCTT TATTTCCAAC 
GAGTTGATTG AAGATGAAAA CGGCGAATTA ACTGAGGTGG AAAGAGAACC TGGTGCCAAC 
GCTATTGGTA TGGTTGCCTT TAAGATTACT GTAAAGACTC CTGAATATCC AAGAGGCCGT 
CAATTTGTTG TTGTTGCTAA CGATATCACA TTCAAGATCG GTTCCTTTGG TCCACAAGAA 
GACGAATTCT TCAATAAGGT TACTGAATAT GCTAGAAAGC GTGGTATCCC AAGAATTTAC 
TTGGCTGCAA ACTCAGGTGC CAGAATTGGT ATGGCTGAAG AGATTGTTCC ACTATTTCAA 
GTTGCATGGA ATGATGCTGC CAATCCGGAC AAGGGCTTCC AATACTTATA CTTAACAAGT 
GAAGGTATGG AAACTTTAAA GAAATTTGAC AAAGAAAATT CTGTTCTCAC TGAACGTACT 
GTTATAAACG GTGAAGAAAG ATTTGTCATC AAGACAATTA TTGGTTCTGA AGATGGGTTA 
GGTGTCGAAT GTCTACGTGG ATCTGGTTTA ATTGCTGGTG CAACGTCAAG GGCTTACCAC 
GATATCTTCA CTATCACCTT AGTCACTTGT AGATCCGTCG GTATCGGTGC TTATTTGGTT 
CGTTTGGGTC AAAGAGCTAT TCAGGTCGAA GGCCAGCCAA TTATTTTAAC TGGTGCTCCT 
GCAATCAACA AAATGCTGGG TAGAGAAGTT TATACTTCTA ACTTACAATT GGGTGGTACT 
CAAATCATGT ATAACAACGG TGTTTCACAT TTGACTGCTG TTGACGATTT AGCTGGTGTA 
GAGAAGATTG TTGAATGGAT GTCTTATGTT CCAGCCAAGC GTAATATGCC AGTTCCTATC 
TTGGAAACTA AAGACACATG GGATAGACCA GTTGATTTCA CTCCAACTAA TGATGAAACT 
TACGATGTAA GATGGATGAT TGAAGGTCGT GAGACTGAAA GTGGATTTGA ATATGGTTTG 
TTTGATAAAG GGTCTTTCTT TGAAACTTTG TCAGGATGGG CCAAAGGTGT TGTCGTTGGT 
AGAGCCCGTC TTGGTGGTAT TCCACTGGGT GTTATTGGTG TTGAAACAAG AACTGTCGAG 
AACTTGATTC CTGCTGATCC AGCTAATCCA AATAGTGCTG AAACATTAAT TCAAGAACCT 
GGTCAAGTTT GGCATCCAAA CTCCGCCTTC AAGACTGCTC AAGCTATCAA TGACTTTAAC 
AACGGTGAAC AATTGCCAAT GATGATTTTG GCCAACTGGA GAGGTTTCTC TGGTGGTCAA 
CGTGATATGT TCAACGAAGT CTTGAAGTAT GGTTCGTTTA TTGTTGACGC ATTGGTGGAT 
TACAAACAAC CAATTATTAT CTATATCCCA CCTACCGGTG AACTAAGAGG TGGTTCATGG 
GTTGTTGTCG ATCCAACTAT CAACGCTGAC CAAATGGAAA TGTATGCCGA CGTCAACGCT 
AGAGCTGGTG TTTTGGAACC ACAAGGTATG GTTGGTATCA AGTTCCGTAG AGAAAAATTG 
CTGGACACCA TGAACAGATT GGATGACAAG TACAGAGAAT TGAGATCTCA ATTATCCAAC 
AAGAGTTTGG CTCCAGAAGT ACATCAGCAA ATATCCAAGC AATTAGCTGA TCGTGAGAGA 
GAACTATTGC CAATTTACGG ACAAATCAGT CTTCAATTTG CTGATTTGCA CGATAGGTCT 
TCACGTATGG TGGCCAAGGG TGTTATTTCT AAGGAACTGG AATGGACCGA GGCACGTCGT 
TTCTTCTTCT GGAGATTGAG AAGAAGATTG AACGAAGAAT ATTTGATTAA AAGGTTGAGC 
CATCAGGTAG GCGAAGCATC AAGATTAGAA AAGATCGCAA GAATTAGATC GTGGTACCCT 
GCTTCAGTGG ACCATGAAGA TGATAGGCAA GTCGCAACAT GGATTGAAGA AAACTACAAA 
ACTTTGGACG ATAAACTAAA GGGTTTGAAA TTAGAGTCAT TCGCTCAAGA CTTAGCTAAA 
AAGATCAGAA GCGACCATGA CAATGCTATT GATGGATTAT CTGAAGTTAT CAAGATGTTA 
TCTACCGATG ATAAAGAAAA ATTGTTGAAG ACTTTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014413.1
CDS	1..6702
Translation	MSEESLFESSPQKMEYEITNYSERHTELPGHFIGLNTVDKLEESPLRDFVKSHGGHTVISKILIANNGIAAVKEIRSVRKWAYETFGDDRTVQFVAMATPEDLEANAEYIRMADQYIEVPGGTNNNNYANVDLIVDIAERADVDAVWAGWGHASENPLLPEKLSQSKRKVIFIGPPGNAMRSLGDKISSTIVAQSAKVPCIPWSGTGVDTVHVDEKTGLVSVDDDIYQKGCCTSPEDGLQKAKRIGFPVMIKASEGGGGKGIRQVEREEDFIALYHQAANEIPGSPIFIMKLAGRARHLEVQLLADQYGTNISLFGRDCSVQRRHQKIIEEAPVTIAKAETFHEMEKAAVRLGKLVGYVSAGTVEYLYSHDDGKFYFLELNPRLQVEHPTTEMVSGVNLPAAQLQIAMGIPMHRISDIRTLYGMNPHSASEIDFEFKTQDATKKQRRPIPKGHCTACRITSEDPNDGFKPSGGTLHELNFRSSSNVWGYFSVGNNGNIHSFSDSQFGHIFAFGENRQASRKHMVVALKELSIRGDFRTTVEYLIKLLETEDFEDNTITTGWLDDLITHKMTAEKPDPTLAVICGAATKAFLASEEARHKYIESLQKGQVLSKDLLQTMFPVDFIHEGKRYKFTVAKSGNDRYTLFINGSKCDIILRQLSDGGLLIAIGGKSHTIYWKEEVAATRLSVDSMTTLLEVENDPTQLRTPSPGKLVKFLVENGEHIIKGQPYAEIEVMKMQMPLVSQENGIVQLLKQPGSTIVAGDIMAIMTLDDPSKVKHALPFEGMLPDFGSPVIEGTKPAYKFKSLVSTLENILKGYDNQVIMNASLQQLIEVLRNPKLPYSEWKLHISALHSRLPAKLDEQMEELVARSLRRGAVFPARQLSKLIDMAVKNPEYNPDKLLGAVVEPLADIAHKYSNGLEAHEHSIFVHFLEEYYEVEKLFNGPNVREENIILKLRDENPKDLDKVALTVLSHSKVSAKNNLILAILKHYQPLCKLSSKVSAIFSTPLQHIVELESKATAKVALQAREILIQGALPSVKERTEQIEHILKSSVVKVAYGSSNPKRSEPDLNILKDLIDSNYVVFDVLLQFLTHQDPVVTAAAAQVYIRRAYRAYTIGDIRVHEGVTVPIVEWKFQLPSAAFSTFPTVKSKMGMNRAVSVSDLSYVANSQSSPLREGILMAVDHLDDVDEILSQSLEVIPRHQSSSNGPAPDRSGSSASLSNVANVCVASTEGFESEEEILVRLREILDLNKQELINASIRRITFMFGFKDGSYPKYYTFNGPNYNENETIRHIEPALAFQLELGRLSNFNIKPIFTDNRNIHVYEAVSKTSPLDKRFFTRGIIRTGHIRDDISIQEYLTSEANRLMSDILDNLEVTDTSNSDLNHIFINFIAVFDISPEDVEAAFGGFLERFGKRLLRLRVSSAEIRIIIKDPQTGAPVPLRALINNVSGYVIKTEMYTEVKNAKGEWVFKSLGKPGSMHLRPIATPYPVKEWLQPKRYKAHLMGTTYVYDFPELFRQASSSQWKNFSADVKLTDDFFISNELIEDENGELTEVEREPGANAIGMVAFKITVKTPEYPRGRQFVVVANDITFKIGSFGPQEDEFFNKVTEYARKRGIPRIYLAANSGARIGMAEEIVPLFQVAWNDAANPDKGFQYLYLTSEGMETLKKFDKENSVLTERTVINGEERFVIKTIIGSEDGLGVECLRGSGLIAGATSRAYHDIFTITLVTCRSVGIGAYLVRLGQRAIQVEGQPIILTGAPAINKMLGREVYTSNLQLGGTQIMYNNGVSHLTAVDDLAGVEKIVEWMSYVPAKRNMPVPILETKDTWDRPVDFTPTNDETYDVRWMIEGRETESGFEYGLFDKGSFFETLSGWAKGVVVGRARLGGIPLGVIGVETRTVENLIPADPANPNSAETLIQEPGQVWHPNSAFKTAQAINDFNNGEQLPMMILANWRGFSGGQRDMFNEVLKYGSFIVDALVDYKQPIIIYIPPTGELRGGSWVVVDPTINADQMEMYADVNARAGVLEPQGMVGIKFRREKLLDTMNRLDDKYRELRSQLSNKSLAPEVHQQISKQLADRERELLPIYGQISLQFADLHDRSSRMVAKGVISKELEWTEARRFFFWRLRRRLNEEYLIKRLSHQVGEASRLEKIARIRSWYPASVDHEDDRQVATWIEENYKTLDDKLKGLKLESFAQDLAKKIRSDHDNAIDGLSEVIKMLSTDDKEKLLKTLK

Target ORF information:

RefSeq Version	NM_001183193.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C acetyl-CoA carboxylase ACC1 (ACC1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183193.1

ORF Insert Sequence:

ATGAGCGAAG AAAGCTTATT CGAGTCTTCT CCACAGAAGA TGGAGTACGA AATTACAAAC 
TACTCAGAAA GACATACAGA ACTTCCAGGT CATTTCATTG GCCTCAATAC AGTAGATAAA 
CTAGAGGAGT CCCCGTTAAG GGACTTTGTT AAGAGTCACG GTGGTCACAC GGTCATATCC 
AAGATCCTGA TAGCAAATAA TGGTATTGCC GCCGTGAAAG AAATTAGATC CGTCAGAAAA 
TGGGCATACG AGACGTTCGG CGATGACAGA ACCGTCCAAT TCGTCGCCAT GGCCACCCCA 
GAAGATCTGG AGGCCAACGC AGAATATATC CGTATGGCCG ATCAATACAT TGAAGTGCCA 
GGTGGTACTA ATAATAACAA CTACGCTAAC GTAGACTTGA TCGTAGACAT CGCCGAAAGA 
GCAGACGTAG ACGCCGTATG GGCTGGCTGG GGTCACGCCT CCGAGAATCC ACTATTGCCT 
GAAAAATTGT CCCAGTCTAA GAGGAAAGTC ATCTTTATTG GGCCTCCAGG TAACGCCATG 
AGGTCTTTAG GTGATAAAAT CTCCTCTACC ATTGTCGCTC AAAGTGCTAA AGTCCCATGT 
ATTCCATGGT CTGGTACCGG TGTTGACACC GTTCACGTGG ACGAGAAAAC CGGTCTGGTC 
TCTGTCGACG ATGACATCTA TCAAAAGGGT TGTTGTACCT CTCCTGAAGA TGGTTTACAA 
AAGGCCAAGC GTATTGGTTT TCCTGTCATG ATTAAGGCAT CCGAAGGTGG TGGTGGTAAA 
GGTATCAGAC AAGTTGAACG TGAAGAAGAT TTCATCGCTT TATACCACCA GGCAGCCAAC 
GAAATTCCAG GCTCCCCCAT TTTCATCATG AAGTTGGCCG GTAGAGCGCG TCACTTGGAA 
GTTCAACTGC TAGCAGATCA GTACGGTACA AATATTTCCT TGTTCGGTAG AGACTGTTCC 
GTTCAGAGAC GTCATCAAAA AATTATCGAA GAAGCACCAG TTACAATTGC CAAGGCTGAA 
ACATTTCACG AGATGGAAAA GGCTGCCGTC AGACTGGGGA AACTAGTCGG TTATGTCTCT 
GCCGGTACCG TGGAGTATCT ATATTCTCAT GATGATGGAA AATTCTACTT TTTAGAATTG 
AACCCAAGAT TACAAGTCGA GCATCCAACA ACGGAAATGG TCTCCGGTGT TAACTTACCT 
GCAGCTCAAT TACAAATCGC TATGGGTATC CCTATGCATA GAATAAGTGA CATTAGAACT 
TTATATGGTA TGAATCCTCA TTCTGCCTCA GAAATCGATT TCGAATTCAA AACTCAAGAT 
GCCACCAAGA AACAAAGAAG ACCTATTCCA AAGGGTCATT GTACCGCTTG TCGTATCACA 
TCAGAAGATC CAAACGATGG ATTCAAGCCA TCGGGTGGTA CTTTGCATGA ACTAAACTTC 
CGTTCTTCCT CTAATGTTTG GGGTTACTTC TCCGTGGGTA ACAATGGTAA TATTCACTCC 
TTTTCGGACT CTCAGTTCGG CCATATTTTT GCTTTTGGTG AAAATAGACA AGCTTCCAGG 
AAACACATGG TTGTTGCCCT GAAGGAATTG TCCATTAGGG GTGATTTCAG AACTACTGTG 
GAATACTTGA TCAAACTTTT GGAAACTGAA GATTTCGAGG ATAACACTAT TACCACCGGT 
TGGTTGGACG ATTTGATTAC TCATAAAATG ACCGCTGAAA AGCCTGATCC AACTCTTGCC 
GTCATTTGCG GTGCCGCTAC AAAGGCTTTC TTAGCATCTG AAGAAGCCCG CCACAAGTAT 
ATCGAATCCT TACAAAAGGG ACAAGTTCTA TCTAAAGACC TACTGCAAAC TATGTTCCCT 
GTAGATTTTA TCCATGAGGG TAAAAGATAC AAGTTCACCG TAGCTAAATC CGGTAATGAC 
CGTTACACAT TATTTATCAA TGGTTCTAAA TGTGATATCA TACTGCGTCA ACTATCTGAT 
GGTGGTCTTT TGATTGCCAT AGGCGGTAAA TCGCATACCA TCTATTGGAA AGAAGAAGTT 
GCTGCTACAA GATTATCCGT TGACTCTATG ACTACTTTGT TGGAAGTTGA AAACGATCCA 
ACCCAGTTGC GTACTCCATC CCCTGGTAAA TTGGTTAAAT TCTTGGTGGA AAATGGTGAA 
CACATTATCA AGGGCCAACC ATATGCAGAA ATTGAAGTTA TGAAAATGCA AATGCCTTTG 
GTTTCTCAAG AAAATGGTAT CGTCCAGTTA TTAAAGCAAC CTGGTTCTAC CATTGTTGCA 
GGTGATATCA TGGCTATTAT GACTCTTGAC GATCCATCCA AGGTCAAGCA CGCTCTACCA 
TTTGAAGGTA TGCTGCCAGA TTTTGGTTCT CCAGTTATCG AAGGAACCAA ACCTGCCTAT 
AAATTCAAGT CATTAGTGTC TACTTTGGAA AACATTTTGA AGGGTTATGA CAACCAAGTT 
ATTATGAACG CTTCCTTGCA ACAATTGATA GAGGTTTTGA GAAATCCAAA ACTGCCTTAC 
TCAGAATGGA AACTACACAT CTCTGCTTTA CATTCAAGAT TGCCTGCTAA GCTAGATGAA 
CAAATGGAAG AGTTAGTTGC ACGTTCTTTG AGACGTGGTG CTGTTTTCCC AGCTAGACAA 
TTAAGTAAAT TGATTGATAT GGCCGTGAAG AATCCTGAAT ACAACCCCGA CAAATTGCTG 
GGCGCCGTCG TGGAACCATT GGCGGATATT GCTCATAAGT ACTCTAACGG GTTAGAAGCC 
CATGAACATT CTATATTTGT CCATTTCTTG GAAGAATATT ACGAAGTTGA AAAGTTATTC 
AATGGTCCAA ATGTTCGTGA GGAAAATATC ATTCTGAAAT TGCGTGATGA AAACCCTAAA 
GATCTAGATA AAGTTGCGCT AACTGTTTTG TCTCATTCGA AAGTTTCAGC GAAGAATAAC 
CTGATCCTAG CTATCTTGAA ACATTATCAA CCATTGTGCA AGTTATCTTC TAAAGTTTCT 
GCCATTTTCT CTACTCCTCT ACAACATATT GTTGAACTAG AATCTAAGGC TACCGCTAAG 
GTCGCTCTAC AAGCAAGAGA AATTTTGATT CAAGGCGCTT TACCTTCGGT CAAGGAAAGA 
ACTGAACAAA TTGAACATAT CTTAAAATCC TCTGTTGTGA AGGTTGCCTA TGGCTCATCC 
AATCCAAAGC GCTCTGAACC AGATTTGAAT ATCTTGAAGG ACTTGATCGA TTCTAATTAC 
GTTGTGTTCG ATGTTTTACT TCAATTCCTA ACCCATCAAG ACCCAGTTGT GACTGCTGCA 
GCTGCTCAAG TCTATATTCG TCGTGCTTAT CGTGCTTACA CCATAGGAGA TATTAGAGTT 
CACGAAGGTG TCACAGTTCC AATTGTTGAA TGGAAATTCC AACTACCTTC AGCTGCGTTC 
TCCACCTTTC CAACTGTTAA ATCTAAAATG GGTATGAACA GGGCTGTTTC TGTTTCAGAT 
TTGTCATATG TTGCAAACAG TCAGTCATCT CCGTTAAGAG AAGGTATTTT GATGGCTGTG 
GATCATTTAG ATGATGTTGA TGAAATTTTG TCACAAAGTT TGGAAGTTAT TCCTCGTCAC 
CAATCTTCTT CTAACGGACC TGCTCCTGAT CGTTCTGGTA GCTCCGCATC GTTGAGTAAT 
GTTGCTAATG TTTGTGTTGC TTCTACAGAA GGTTTCGAAT CTGAAGAGGA AATTTTGGTA 
AGGTTGAGAG AAATTTTGGA TTTGAATAAG CAGGAATTAA TCAATGCTTC TATCCGTCGT 
ATCACATTTA TGTTCGGTTT TAAAGATGGG TCTTATCCAA AGTATTATAC TTTTAACGGT 
CCAAATTATA ACGAAAATGA AACAATTCGT CACATTGAGC CGGCTTTGGC CTTCCAACTG 
GAATTAGGAA GATTGTCCAA CTTCAACATT AAACCAATTT TCACTGATAA TAGAAACATC 
CATGTCTACG AAGCTGTTAG TAAGACTTCT CCATTGGATA AGAGATTCTT TACAAGAGGT 
ATTATTAGAA CGGGTCATAT CCGTGATGAC ATTTCTATTC AAGAATATCT GACTTCTGAA 
GCTAACAGAT TGATGAGTGA TATATTGGAT AATTTAGAAG TCACCGACAC TTCAAATTCT 
GATTTGAATC ATATCTTCAT CAACTTCATT GCGGTGTTTG ATATCTCTCC AGAAGATGTC 
GAAGCCGCCT TCGGTGGTTT CTTAGAAAGA TTTGGTAAGA GATTGTTGAG ATTGCGTGTT 
TCTTCTGCCG AAATTAGAAT CATCATCAAA GATCCTCAAA CAGGTGCCCC AGTACCATTG 
CGTGCCTTGA TCAATAACGT TTCTGGTTAT GTTATCAAAA CAGAAATGTA CACCGAAGTC 
AAGAACGCAA AAGGTGAATG GGTATTTAAG TCTTTGGGTA AACCTGGATC CATGCATTTA 
AGACCTATTG CTACTCCTTA CCCTGTTAAG GAATGGTTGC AACCAAAACG TTATAAGGCA 
CACTTGATGG GTACCACATA TGTCTATGAC TTCCCAGAAT TATTCCGCCA AGCATCGTCA 
TCCCAATGGA AAAATTTCTC TGCAGATGTT AAGTTAACAG ATGATTTCTT TATTTCCAAC 
GAGTTGATTG AAGATGAAAA CGGCGAATTA ACTGAGGTGG AAAGAGAACC TGGTGCCAAC 
GCTATTGGTA TGGTTGCCTT TAAGATTACT GTAAAGACTC CTGAATATCC AAGAGGCCGT 
CAATTTGTTG TTGTTGCTAA CGATATCACA TTCAAGATCG GTTCCTTTGG TCCACAAGAA 
GACGAATTCT TCAATAAGGT TACTGAATAT GCTAGAAAGC GTGGTATCCC AAGAATTTAC 
TTGGCTGCAA ACTCAGGTGC CAGAATTGGT ATGGCTGAAG AGATTGTTCC ACTATTTCAA 
GTTGCATGGA ATGATGCTGC CAATCCGGAC AAGGGCTTCC AATACTTATA CTTAACAAGT 
GAAGGTATGG AAACTTTAAA GAAATTTGAC AAAGAAAATT CTGTTCTCAC TGAACGTACT 
GTTATAAACG GTGAAGAAAG ATTTGTCATC AAGACAATTA TTGGTTCTGA AGATGGGTTA 
GGTGTCGAAT GTCTACGTGG ATCTGGTTTA ATTGCTGGTG CAACGTCAAG GGCTTACCAC 
GATATCTTCA CTATCACCTT AGTCACTTGT AGATCCGTCG GTATCGGTGC TTATTTGGTT 
CGTTTGGGTC AAAGAGCTAT TCAGGTCGAA GGCCAGCCAA TTATTTTAAC TGGTGCTCCT 
GCAATCAACA AAATGCTGGG TAGAGAAGTT TATACTTCTA ACTTACAATT GGGTGGTACT 
CAAATCATGT ATAACAACGG TGTTTCACAT TTGACTGCTG TTGACGATTT AGCTGGTGTA 
GAGAAGATTG TTGAATGGAT GTCTTATGTT CCAGCCAAGC GTAATATGCC AGTTCCTATC 
TTGGAAACTA AAGACACATG GGATAGACCA GTTGATTTCA CTCCAACTAA TGATGAAACT 
TACGATGTAA GATGGATGAT TGAAGGTCGT GAGACTGAAA GTGGATTTGA ATATGGTTTG 
TTTGATAAAG GGTCTTTCTT TGAAACTTTG TCAGGATGGG CCAAAGGTGT TGTCGTTGGT 
AGAGCCCGTC TTGGTGGTAT TCCACTGGGT GTTATTGGTG TTGAAACAAG AACTGTCGAG 
AACTTGATTC CTGCTGATCC AGCTAATCCA AATAGTGCTG AAACATTAAT TCAAGAACCT 
GGTCAAGTTT GGCATCCAAA CTCCGCCTTC AAGACTGCTC AAGCTATCAA TGACTTTAAC 
AACGGTGAAC AATTGCCAAT GATGATTTTG GCCAACTGGA GAGGTTTCTC TGGTGGTCAA 
CGTGATATGT TCAACGAAGT CTTGAAGTAT GGTTCGTTTA TTGTTGACGC ATTGGTGGAT 
TACAAACAAC CAATTATTAT CTATATCCCA CCTACCGGTG AACTAAGAGG TGGTTCATGG 
GTTGTTGTCG ATCCAACTAT CAACGCTGAC CAAATGGAAA TGTATGCCGA CGTCAACGCT 
AGAGCTGGTG TTTTGGAACC ACAAGGTATG GTTGGTATCA AGTTCCGTAG AGAAAAATTG 
CTGGACACCA TGAACAGATT GGATGACAAG TACAGAGAAT TGAGATCTCA ATTATCCAAC 
AAGAGTTTGG CTCCAGAAGT ACATCAGCAA ATATCCAAGC AATTAGCTGA TCGTGAGAGA 
GAACTATTGC CAATTTACGG ACAAATCAGT CTTCAATTTG CTGATTTGCA CGATAGGTCT 
TCACGTATGG TGGCCAAGGG TGTTATTTCT AAGGAACTGG AATGGACCGA GGCACGTCGT 
TTCTTCTTCT GGAGATTGAG AAGAAGATTG AACGAAGAAT ATTTGATTAA AAGGTTGAGC 
CATCAGGTAG GCGAAGCATC AAGATTAGAA AAGATCGCAA GAATTAGATC GTGGTACCCT 
GCTTCAGTGG ACCATGAAGA TGATAGGCAA GTCGCAACAT GGATTGAAGA AAACTACAAA 
ACTTTGGACG ATAAACTAAA GGGTTTGAAA TTAGAGTCAT TCGCTCAAGA CTTAGCTAAA 
AAGATCAGAA GCGACCATGA CAATGCTATT GATGGATTAT CTGAAGTTAT CAAGATGTTA 
TCTACCGATG ATAAAGAAAA ATTGTTGAAG ACTTTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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ACC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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