ACACA gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_005624777.3	XP_005624834.1	acetyl-CoA carboxylase 1 isoform X3
XM_005624780.3	XP_005624837.1	acetyl-CoA carboxylase 1 isoform X4
XM_005624782.3	XP_005624839.2	acetyl-CoA carboxylase 1 isoform X6
XM_005624776.2	XP_005624833.1	acetyl-CoA carboxylase 1 isoform X2
XM_005624780.2	XP_005624837.1	acetyl-CoA carboxylase 1 isoform X4
XM_005624782.2	XP_005624839.1	acetyl-CoA carboxylase 1 isoform X6
XM_005624777.2	XP_005624834.1	acetyl-CoA carboxylase 1 isoform X3
XM_005624783.2	XP_005624840.1	acetyl-CoA carboxylase 1 isoform X4
XM_005624778.2	XP_005624835.1	acetyl-CoA carboxylase 1 isoform X4
XM_005624779.3	XP_005624836.1	acetyl-CoA carboxylase 1 isoform X4
XM_005624775.2	XP_005624832.1	acetyl-CoA carboxylase 1 isoform X1
XM_005624779.2	XP_005624836.1	acetyl-CoA carboxylase 1 isoform X4
XM_548250.5	XP_548250.3	acetyl-CoA carboxylase 1 isoform X5
XM_005624783.3	XP_005624840.1	acetyl-CoA carboxylase 1 isoform X4
XM_005624778.3	XP_005624835.1	acetyl-CoA carboxylase 1 isoform X4
XM_005624775.3	XP_005624832.1	acetyl-CoA carboxylase 1 isoform X1
XM_005624776.3	XP_005624833.1	acetyl-CoA carboxylase 1 isoform X2
XM_548250.6	XP_548250.3	acetyl-CoA carboxylase 1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe290752	XM_005624782.3 Latest version!	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe12090	XM_005624775.3 Latest version!	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe12091	XM_005624776.3 Latest version!	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe12092	XM_005624777.3 Latest version!	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe12093	XM_005624779.3 Latest version!	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe12093	XM_005624780.3 Latest version!	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe12093	XM_005624783.3 Latest version!	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe12093	XM_005624778.3 Latest version!	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe12094	XM_548250.6 Latest version!	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe12095	XM_005624782.2 Latest version!	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OCe12093	XM_005624780.2	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe12092	XM_005624777.2	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe12093	XM_005624779.2	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe12093	XM_005624783.2	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe12093	XM_005624778.2	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe12090	XM_005624775.2	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe12091	XM_005624776.2	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OCe12094	XM_548250.5	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

ACACA ( XM_005624782.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624839.2 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCe290752
Clone ID Related Accession (Same CDS sequence)	XM_005624782.3
Accession Version	XM_005624782.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	acetyl-CoA carboxylase 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624782.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGCT TGCATCTAGT GAAGCAAGGT CGAGACCGAA AGAAAATAGA CTCTCAGCGA 
GATTTCACTG TAGCTTCTCC AGCAGAATTT GTTACTCGTT TTGGGGGAAA TAAAGTGATT 
GAGAAGGTTC TTATTGCCAA CAATGGCATT GCAGCAGTGA AATGCATGCG GTCTATCCGT 
CGATGGTCTT ATGAGATGTT TCGAAATGAA CGTGCAATCC GATTTGTTGT TATGGTAACA 
CCTGAAGACC TGAAAGCCAA TGCAGAATAC ATTAAGATGG CGGATCACTA TGTGCCAGTG 
CCAGGAGGAC CAAATAACAA CAACTATGCG AACGTGGAAT TAATTCTTGA TATTGCTAAA 
AGGATTCCCG TACAAGCAGT GTGGGCTGGC TGGGGTCATG CTTCTGAGAA TCCCAAGCTC 
CCAGAACTTC TTTTGAAAAA TGGCATCGCC TTCATGGGCC CTCCAAGCCA GGCCATGTGG 
GCTTTGGGGG ATAAGATTGC ATCTTCTATA GTGGCTCAAA CTGCAGGTAT CCCAACTCTT 
CCCTGGAGTG GCAGTGGTCT TCGGGTGGAC TGGCATGAAA ATGATTTTTC CAAACGTATC 
TTAAATGTTC CCCAGGAATT ATACGAAAAA GGTTATGTGA AGGATGTGGA TGATGGGCTA 
AAGGCAGCGG AGGAAGTTGG ATATCCAGTA ATGATCAAGG CCTCAGAAGG AGGAGGAGGG 
AAAGGAATCA GAAAAGTCAA CAATGCAGAT GATTTCCCTA ACCTCTTCAG ACAGGTTCAA 
GCTGAAGTCC CTGGATCTCC TATATTTGTG ATGAGACTAG CCAAACAATC TCGTCATCTG 
GAGGTACAGA TCTTAGCAGA TCAATATGGC AATGCTATCT CTCTCTTTGG TCGTGACTGC 
TCTGTACAGC GCAGGCATCA GAAGATTATT GAGGAAGCAC CTGCTGCTAT TGCTACTCCA 
GCAGTATTTG AACATATGGA ACAGTGTGCT GTGAAACTTG CCAAAATGGT TGGTTATGTG 
AGTGCTGGGA CTGTGGAATA CCTCTACAGC CAGGATGGAA GCTTCTACTT TCTGGAACTG 
AACCCTCGGC TACAGGTGGA ACACCCTTGT ACAGAGATGG TGGCTGATGT CAACCTCCCT 
GCAGCACAAC TCCAGATTGC CATGGGGATC CCTCTGTACA GAATCAAGGA TATTCGTATG 
ATGTATGGGG TATCTCCCTG GGGTGATGCT CCCATTGATT TTGAAAATTC TGCTCACGTT 
CCTTGCCCAA GGGGCCATGT TATTGCCGCT CGGATCACTA GTGAAAATCC AGATGAGGGT 
TTTAAGCCCA GCTCAGGAAC AGTTCAGGAA CTGAATTTCC GTAGCAATAA GAATGTTTGG 
GGTTATTTCA GTGTTGCTGC TGCAGGGGGC CTTCATGAAT TTGCTGATTC TCAGTTTGGT 
CATTGCTTCT CCTGGGGAGA AAACAGAGAG GAAGCAATTT CAAACATGGT GGTGGCTTTG 
AAGGAGCTGT CTATTCGGGG TGACTTTCGA ACCACAGTTG AATACCTCAT CAAATTGTTG 
GAGACTGAAA GCTTTCAGTT GAATAGAATT GACACCGGCT GGCTGGACAG ACTGATAGCA 
GAGAAAGTAC AGGCAGAGCG ACCTGACACC ATGTTGGGAG TTGTTTGTGG GGCTCTCCAT 
GTGGCAGATG TGAGCCTACG GAATAGCATC TCTAACTTCC TTCATTCTTT AGAAAGGGGT 
CAAGTCCTTC CTGCTCATAC ACTTCTGAAT ACAGTAGATG TTGAACTTAT CTATGGGGGA 
GTCAAATATG TACTTAAGGT GACTCGGCAG TCCCCCAACT CCTATGTGGT GATCATGAAT 
GGTTCATGCG TAGAAGTAGA TGTGCATCGG CTCAGTGATG GAGGGTTACT CCTGTCCTAT 
GATGGCAGCA GTTACACCAC ATACATGAAG GAAGAAGTGG ATAGATATCG TATCACAATT 
GGCAATAAGA CCTGTGTGTT TGAGAAGGAA AATGACCCTT CAGTGTTACG CTCACCTTCT 
GCTGGGAAGT TAATCCAGTA CATTGTGGAG GATGGAGGCC ATGTGTTTGC TGGCCAGTGC 
TATGCTGAAA TTGAGGTGAT GAAGATGGTA ATGACCTTAA CAGCTGCAGA GTCTGGCTGT 
ATCCATTATG TCAAACGGCC TGGAGCAGCT CTTGACCCTG GCTGTGTAAT AGCCAAAATG 
CAGTTGGACA ACCCCAGCAA GGTCCAACAG GCTGAGCTTC ACACAGGTAG TCTGCCACGG 
ATCCAGAGCA CTGCACTCAG AGGCGAGAAG CTCCATCGAG TGTTCCACTA TGTCCTGGAT 
AATCTGGTCA ACGTAATGAA TGGATACTGC CTTCCAGATC CTTTTTTTAG CAGCAGGGTA 
AAAGACTGGG TAGAGCGGTT GATGAAGACC CTCAGAGACC CCTCGTTACC TCTCCTAGAA 
TTACAAGATA TCATGACCAG TGTCTCTGGC CGTATTCCCC CCAATGTTGA GAAGTCTATC 
AAGAAGGAAA TGGCCCAGTA TGCCAGCAAT ATCACATCAG TCCTCTGTCA GTTTCCCAGC 
CAGCAGATTG CCAACATCCT AGATAGCCAT GCAGCTACGT TGAACCGGAA ATCTGAGCGG 
GAAGTCTTTT TCATGAACAC TCAGAGCATT GTCCAGCTGG TACAGAGATA CCGAAGTGGC 
ATCCGAGGCC ACATGAAGGC TGTGGTGATG GATCTGCTGC GGCAGTACCT GCGAGTAGAG 
ACACAGTTCC AGAATGGTCA CTATGACAAA TGTGTGTTTG CCCTTCGAGA AGAGAACAAG 
AGTGATATGA ACACTGTACT GAACTATATC TTCTCTCATG CTCAAGTCAC CAAGAAGAAT 
CTTCTGGTCA CAATGCTTAT TGATCAGTTG TGCGGTAGGG ACCCTACTCT CACTGATGAG 
CTGCTGAATA TTCTCACAGA GCTAACTCAA CTCAGTAAGA CCACCAATGC CAAAGTAGCA 
CTTCGAGCAC GCCAGGTTCT TATTGCCTCC CATTTGCCAT CATATGAGCT TCGCCATAAC 
CAAGTAGAGT CTATCTTCCT TTCAGCTATT GACATGTATG GACATCAGTT TTGCATTGAG 
AACCTGCAGA AACTCATCTT GTCTGAAACA TCTATTTTTG ATGTCCTACC AAACTTCTTC 
TATCACAGCA ACCAGGTAGT GAGGATGGCA GCTCTGGAGG TTTATGTTCG AAGGGCTTAT 
ATTGCCTATG AACTTAACAG CGTACAACAT CGCCAACTTA AGGACAACAC CTGTGTGGTG 
GAATTCCAAT TCATGCTACC CACATCTCAT CCAAATAGAG GGAACATCCC CACGCTAAAC 
AGAATGTCCT TCTCCTCCAA CCTCAACCAC TATGGCATGA CTCATGTAGC TAGTGTCAGC 
GATGTGCTTT TGGACAACTC ATTCACTCCA CCATGTCAAC GGATGGGCGG AATGGTCTCT 
TTTCGGACTT TCGAAGATTT TGTCAGGATC TTTGATGAAG TGATGGGCTG CTTCTGTGAT 
TCTCCACCGC AAAGCCCTAC ATTCCCTGAG GCAGGTCACA CATCTCTATA CGATGAAGAC 
AAGGTCCCCA GGGATGAACC AATTCACATT CTGAATGTGG CTATCAAGAC TGACTGTGAT 
ATTGAGGATG ACAGACTGGC AGCTATGTTC AGAGAGTTTA CCCAACAAAA TAAAGCTACC 
CTGGTTGAGC ATGGGATACG GCGCCTTACT TTCCTGGTTG CACAAAAGGA TTTCAGGAAA 
CAGGTCAACT ATGAGGTGGA TCAGAGATTT CATAGAGAAT TTCCTAAATT TTTCACCTTC 
CGAGCAAGGG ATAAGTTTGA GGAGGATCGT ATCTATCGTC ACCTGGAACC TGCCCTCGCT 
TTCCAGTTAG AGCTGAACCG GATGAGAAAT TTTGACCTTA CTGCCATTCC ATGTGCTAAT 
CATAAAATGC ATCTGTATCT TGGGGCAGCC AAGGTGGAGG TGGGCACAGA AGTGACAGAC 
TACAGGTTCT TTGTTCGTGC AATCATCAGA CATTCTGATC TGGTCACCAA GGAAGCTTCT 
TTTGAATACC TACAAAATGA AGGGGAGAGG CTGCTTCTAG AAGCCATGGA TGAGTTGGAA 
GTTGCTTTTA ACAATACAAA CGTTCGCACT GACTGTAACC ACATCTTCCT CAACTTTGTG 
CCCACGGTCA TCATGGACCC ATCAAAGATT GAGGAATCTG TGCGGAGCAT GGTAATGCGC 
TATGGAAGTC GGCTGTGGAA ATTACGTGTC CTCCAGGCAG AACTGAAAAT CAACATTCGC 
CTGACACCAA CTGGAAAAGC AATTCCCATC CGCCTCTTCC TGACAAACGA GTCTGGCTAT 
TACTTGGACA TCAGCTTGTA TAAGGAAGTG ACTGACTCCA GGACAGCACA GATCATGTTT 
CAGGCATATG GAGACAAACA GGGACCATTG CATGGAATGT TAATCAACAC TCCATACGTC 
ACCAAAGACC TGCTTCAATC AAAGAGATTC CAGGCACAAT CCTTGGGGAC AACATACATA 
TATGATATAC CAGAGATGTT TCGGCAGTCT CTGATCAAAC TCTGGGAGTC TATGTCCACT 
CAAGCATTTC TTCCATCACC CCCCCTGCCT TCCGACATGC TGACATATAC GGAGCTCGTG 
TTGGATGATC AAGGTCAGCT GGTCCACATG AACAGGCTTC CAGGAGGAAA TGAGATTGGC 
ATGGTAGCGT GGAAAATGAC CCTTAAAAGT CCTGAATACC CAGAAGGCCG AGATATCATT 
GTTATTGGCA ATGACATCAC ATACCGAATT GGGTCCTTTG GGCCCCAGGA GGATTTGCTG 
TTTCTCAGAG CTTCTGAGCT TGCCAGGGCA GAGGGTATCC CACGCATCTT TGTAGCAGCC 
AACAGTGGAG CAAGAATTGG ACTGGCAGAG GAAATTCGTC ATATGTTTCA TGTAGCCTGG 
GTAGATCCTG AGGATCCTTA CAAGGGATAC AAATATTTAT ATCTGACCCC TCAGGATTAT 
AAGAGAGTCA GTGCTCTCAA CTCTGTCCAT TGTGAACATG TAGAGGACGA AGGAGAATCC 
AGGTACAAGA TAACTGATAT TATTGGGAAG GAAGAGGGAC TTGGAGCAGA GAACCTTCGA 
GGCTCTGGAA TGATCGCTGG AGAATCCTCA TTGGCCTATG ATGAGATCAT CACCATCAGC 
CTGGTTACAT GCCGGGCCAT TGGGATTGGA GCTTACCTTG TCCGGCTGGG ACAGAGAACC 
ATCCAGGTTG AAAATTCTCA TTTAATTTTG ACTGGAGCTG GAGCCCTCAA CAAAGTCCTT 
GGGCGGGAAG TATACACCTC CAATAACCAG CTCGGGGGCA TCCAGATCAT GCACAACAAT 
GGGGTGACCC ACAGCATTGT TTGTGATGAC TTTGAAGGGG TTTTCACTGT CCTGCACTGG 
CTATCCTACA TGCCTAAGAA TGTGCGCAGT TTAGTTCCTC TCCTGAACTC CAAGGATCCT 
ATAGATAGAA TCATCGAGTT TGTTCCCACG AAGGCCCCAT ACGATCCTCG ATGGATGCTA 
GCAGGCCGCC CTCACCCAAC TCAGAAAGGT CAGTGGCTGA GTGGATTTTT TGACTATGGC 
TCTTTCTCAG AGATCATGCA ACCCTGGGCA CAAACTGTGG TGGTTGGCAG AGCCAGGCTA 
GGAGGAATAC CTGTGGGAGT AGTTGCTGTA GAAACCCGAA CAGTGGAACT AAGTATCCCA 
GCCGATCCTG CAAACCTGGA TTCTGAAGCC AAGATAATCC AGCAGGCTGG CCAGGTTTGG 
TTCCCAGACT CTGCGTTTAA GACCTATCAG GCTATCAAGG ACTTCAACCG TGAAGGACTG 
CCTCTGATGG TCTTTGCCAA CTGGAGAGGC TTCTCTGGTG GGATGAAAGA TATGTATGAC 
CAAGTGCTGA AGTTTGGTGC CTATATTGTG GATAGCTTAC GGGAGTGCTC CCAGCCTGTG 
CTGGTATACA TTCCTCCTCA GGCTGAGCTC CGGGGTGGCT CCTGGGTTGT GATTGACCCC 
ACCATCAACC CCCGGCATAT GGAGATGTAC GCTGACCGAG AAAGCAGGGG ATCAGTTCTG 
GAGCCAGAAG GGACAGTAGA AATCAAATTC CGCAGAAAGG ATCTGGTGAA AACCATGCGT 
CGGGTGGACC CAGTCTACAT TCGCTTGGCT GAGCGATTGG GCACCCCAGA GCTCAGCGCA 
GCTGAGCGGA AGGAGCTGGA GAACAAGTTG AAGGAGCGGG AGGAATTCCT AATTCCCATT 
TACCATCAGG TAGCCGTGCA GTTTGCTGAC TTGCACGACA CACCAGGCCG GATGCAGGAG 
AAGGGTGTTA TTAATGACAT CCTGGATTGG AAAACATCCC GTACCTTTTT CTATTGGCGG 
CTGAGGCGTC TCCTGCTGGA GGACCTGGTC AAAAAGAAAA TCCACAATGC CAACCCAGAG 
CTGACCGATG GCCAGATCCA GGCCATGCTA CGGCGCTGGT TTGTAGAAGT GGAAGGAACT 
GTGAAGGCCT ACGTTTGGGA CAATAACAAG GACCTGGTGG AGTGGCTGGA GAAACAGCTG 
ACCGAGGAAG ATGGTGTTCG CTCAGTAATC GAGGAAAACA TCAAATATAT CAGCAGAGAC 
TATGTCCTCA AGCAGATCCG CAGCTTGGTA CAGGCCAATC CCGAGGTTGC CATGGACTCC 
ATCGTCCATA TGACTCAGCA TATCTCACCC ACTCAGCGAG CAGAAGTCGT ACGGATCCTC 
TCCACGATGG ACTCCCCTTC AACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624839.2
CDS	27..6833
Translation	MSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_005624782.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624782.3

ORF Insert Sequence:

ATGTCTGGCT TGCATCTAGT GAAGCAAGGT CGAGACCGAA AGAAAATAGA CTCTCAGCGA 
GATTTCACTG TAGCTTCTCC AGCAGAATTT GTTACTCGTT TTGGGGGAAA TAAAGTGATT 
GAGAAGGTTC TTATTGCCAA CAATGGCATT GCAGCAGTGA AATGCATGCG GTCTATCCGT 
CGATGGTCTT ATGAGATGTT TCGAAATGAA CGTGCAATCC GATTTGTTGT TATGGTAACA 
CCTGAAGACC TGAAAGCCAA TGCAGAATAC ATTAAGATGG CGGATCACTA TGTGCCAGTG 
CCAGGAGGAC CAAATAACAA CAACTATGCG AACGTGGAAT TAATTCTTGA TATTGCTAAA 
AGGATTCCCG TACAAGCAGT GTGGGCTGGC TGGGGTCATG CTTCTGAGAA TCCCAAGCTC 
CCAGAACTTC TTTTGAAAAA TGGCATCGCC TTCATGGGCC CTCCAAGCCA GGCCATGTGG 
GCTTTGGGGG ATAAGATTGC ATCTTCTATA GTGGCTCAAA CTGCAGGTAT CCCAACTCTT 
CCCTGGAGTG GCAGTGGTCT TCGGGTGGAC TGGCATGAAA ATGATTTTTC CAAACGTATC 
TTAAATGTTC CCCAGGAATT ATACGAAAAA GGTTATGTGA AGGATGTGGA TGATGGGCTA 
AAGGCAGCGG AGGAAGTTGG ATATCCAGTA ATGATCAAGG CCTCAGAAGG AGGAGGAGGG 
AAAGGAATCA GAAAAGTCAA CAATGCAGAT GATTTCCCTA ACCTCTTCAG ACAGGTTCAA 
GCTGAAGTCC CTGGATCTCC TATATTTGTG ATGAGACTAG CCAAACAATC TCGTCATCTG 
GAGGTACAGA TCTTAGCAGA TCAATATGGC AATGCTATCT CTCTCTTTGG TCGTGACTGC 
TCTGTACAGC GCAGGCATCA GAAGATTATT GAGGAAGCAC CTGCTGCTAT TGCTACTCCA 
GCAGTATTTG AACATATGGA ACAGTGTGCT GTGAAACTTG CCAAAATGGT TGGTTATGTG 
AGTGCTGGGA CTGTGGAATA CCTCTACAGC CAGGATGGAA GCTTCTACTT TCTGGAACTG 
AACCCTCGGC TACAGGTGGA ACACCCTTGT ACAGAGATGG TGGCTGATGT CAACCTCCCT 
GCAGCACAAC TCCAGATTGC CATGGGGATC CCTCTGTACA GAATCAAGGA TATTCGTATG 
ATGTATGGGG TATCTCCCTG GGGTGATGCT CCCATTGATT TTGAAAATTC TGCTCACGTT 
CCTTGCCCAA GGGGCCATGT TATTGCCGCT CGGATCACTA GTGAAAATCC AGATGAGGGT 
TTTAAGCCCA GCTCAGGAAC AGTTCAGGAA CTGAATTTCC GTAGCAATAA GAATGTTTGG 
GGTTATTTCA GTGTTGCTGC TGCAGGGGGC CTTCATGAAT TTGCTGATTC TCAGTTTGGT 
CATTGCTTCT CCTGGGGAGA AAACAGAGAG GAAGCAATTT CAAACATGGT GGTGGCTTTG 
AAGGAGCTGT CTATTCGGGG TGACTTTCGA ACCACAGTTG AATACCTCAT CAAATTGTTG 
GAGACTGAAA GCTTTCAGTT GAATAGAATT GACACCGGCT GGCTGGACAG ACTGATAGCA 
GAGAAAGTAC AGGCAGAGCG ACCTGACACC ATGTTGGGAG TTGTTTGTGG GGCTCTCCAT 
GTGGCAGATG TGAGCCTACG GAATAGCATC TCTAACTTCC TTCATTCTTT AGAAAGGGGT 
CAAGTCCTTC CTGCTCATAC ACTTCTGAAT ACAGTAGATG TTGAACTTAT CTATGGGGGA 
GTCAAATATG TACTTAAGGT GACTCGGCAG TCCCCCAACT CCTATGTGGT GATCATGAAT 
GGTTCATGCG TAGAAGTAGA TGTGCATCGG CTCAGTGATG GAGGGTTACT CCTGTCCTAT 
GATGGCAGCA GTTACACCAC ATACATGAAG GAAGAAGTGG ATAGATATCG TATCACAATT 
GGCAATAAGA CCTGTGTGTT TGAGAAGGAA AATGACCCTT CAGTGTTACG CTCACCTTCT 
GCTGGGAAGT TAATCCAGTA CATTGTGGAG GATGGAGGCC ATGTGTTTGC TGGCCAGTGC 
TATGCTGAAA TTGAGGTGAT GAAGATGGTA ATGACCTTAA CAGCTGCAGA GTCTGGCTGT 
ATCCATTATG TCAAACGGCC TGGAGCAGCT CTTGACCCTG GCTGTGTAAT AGCCAAAATG 
CAGTTGGACA ACCCCAGCAA GGTCCAACAG GCTGAGCTTC ACACAGGTAG TCTGCCACGG 
ATCCAGAGCA CTGCACTCAG AGGCGAGAAG CTCCATCGAG TGTTCCACTA TGTCCTGGAT 
AATCTGGTCA ACGTAATGAA TGGATACTGC CTTCCAGATC CTTTTTTTAG CAGCAGGGTA 
AAAGACTGGG TAGAGCGGTT GATGAAGACC CTCAGAGACC CCTCGTTACC TCTCCTAGAA 
TTACAAGATA TCATGACCAG TGTCTCTGGC CGTATTCCCC CCAATGTTGA GAAGTCTATC 
AAGAAGGAAA TGGCCCAGTA TGCCAGCAAT ATCACATCAG TCCTCTGTCA GTTTCCCAGC 
CAGCAGATTG CCAACATCCT AGATAGCCAT GCAGCTACGT TGAACCGGAA ATCTGAGCGG 
GAAGTCTTTT TCATGAACAC TCAGAGCATT GTCCAGCTGG TACAGAGATA CCGAAGTGGC 
ATCCGAGGCC ACATGAAGGC TGTGGTGATG GATCTGCTGC GGCAGTACCT GCGAGTAGAG 
ACACAGTTCC AGAATGGTCA CTATGACAAA TGTGTGTTTG CCCTTCGAGA AGAGAACAAG 
AGTGATATGA ACACTGTACT GAACTATATC TTCTCTCATG CTCAAGTCAC CAAGAAGAAT 
CTTCTGGTCA CAATGCTTAT TGATCAGTTG TGCGGTAGGG ACCCTACTCT CACTGATGAG 
CTGCTGAATA TTCTCACAGA GCTAACTCAA CTCAGTAAGA CCACCAATGC CAAAGTAGCA 
CTTCGAGCAC GCCAGGTTCT TATTGCCTCC CATTTGCCAT CATATGAGCT TCGCCATAAC 
CAAGTAGAGT CTATCTTCCT TTCAGCTATT GACATGTATG GACATCAGTT TTGCATTGAG 
AACCTGCAGA AACTCATCTT GTCTGAAACA TCTATTTTTG ATGTCCTACC AAACTTCTTC 
TATCACAGCA ACCAGGTAGT GAGGATGGCA GCTCTGGAGG TTTATGTTCG AAGGGCTTAT 
ATTGCCTATG AACTTAACAG CGTACAACAT CGCCAACTTA AGGACAACAC CTGTGTGGTG 
GAATTCCAAT TCATGCTACC CACATCTCAT CCAAATAGAG GGAACATCCC CACGCTAAAC 
AGAATGTCCT TCTCCTCCAA CCTCAACCAC TATGGCATGA CTCATGTAGC TAGTGTCAGC 
GATGTGCTTT TGGACAACTC ATTCACTCCA CCATGTCAAC GGATGGGCGG AATGGTCTCT 
TTTCGGACTT TCGAAGATTT TGTCAGGATC TTTGATGAAG TGATGGGCTG CTTCTGTGAT 
TCTCCACCGC AAAGCCCTAC ATTCCCTGAG GCAGGTCACA CATCTCTATA CGATGAAGAC 
AAGGTCCCCA GGGATGAACC AATTCACATT CTGAATGTGG CTATCAAGAC TGACTGTGAT 
ATTGAGGATG ACAGACTGGC AGCTATGTTC AGAGAGTTTA CCCAACAAAA TAAAGCTACC 
CTGGTTGAGC ATGGGATACG GCGCCTTACT TTCCTGGTTG CACAAAAGGA TTTCAGGAAA 
CAGGTCAACT ATGAGGTGGA TCAGAGATTT CATAGAGAAT TTCCTAAATT TTTCACCTTC 
CGAGCAAGGG ATAAGTTTGA GGAGGATCGT ATCTATCGTC ACCTGGAACC TGCCCTCGCT 
TTCCAGTTAG AGCTGAACCG GATGAGAAAT TTTGACCTTA CTGCCATTCC ATGTGCTAAT 
CATAAAATGC ATCTGTATCT TGGGGCAGCC AAGGTGGAGG TGGGCACAGA AGTGACAGAC 
TACAGGTTCT TTGTTCGTGC AATCATCAGA CATTCTGATC TGGTCACCAA GGAAGCTTCT 
TTTGAATACC TACAAAATGA AGGGGAGAGG CTGCTTCTAG AAGCCATGGA TGAGTTGGAA 
GTTGCTTTTA ACAATACAAA CGTTCGCACT GACTGTAACC ACATCTTCCT CAACTTTGTG 
CCCACGGTCA TCATGGACCC ATCAAAGATT GAGGAATCTG TGCGGAGCAT GGTAATGCGC 
TATGGAAGTC GGCTGTGGAA ATTACGTGTC CTCCAGGCAG AACTGAAAAT CAACATTCGC 
CTGACACCAA CTGGAAAAGC AATTCCCATC CGCCTCTTCC TGACAAACGA GTCTGGCTAT 
TACTTGGACA TCAGCTTGTA TAAGGAAGTG ACTGACTCCA GGACAGCACA GATCATGTTT 
CAGGCATATG GAGACAAACA GGGACCATTG CATGGAATGT TAATCAACAC TCCATACGTC 
ACCAAAGACC TGCTTCAATC AAAGAGATTC CAGGCACAAT CCTTGGGGAC AACATACATA 
TATGATATAC CAGAGATGTT TCGGCAGTCT CTGATCAAAC TCTGGGAGTC TATGTCCACT 
CAAGCATTTC TTCCATCACC CCCCCTGCCT TCCGACATGC TGACATATAC GGAGCTCGTG 
TTGGATGATC AAGGTCAGCT GGTCCACATG AACAGGCTTC CAGGAGGAAA TGAGATTGGC 
ATGGTAGCGT GGAAAATGAC CCTTAAAAGT CCTGAATACC CAGAAGGCCG AGATATCATT 
GTTATTGGCA ATGACATCAC ATACCGAATT GGGTCCTTTG GGCCCCAGGA GGATTTGCTG 
TTTCTCAGAG CTTCTGAGCT TGCCAGGGCA GAGGGTATCC CACGCATCTT TGTAGCAGCC 
AACAGTGGAG CAAGAATTGG ACTGGCAGAG GAAATTCGTC ATATGTTTCA TGTAGCCTGG 
GTAGATCCTG AGGATCCTTA CAAGGGATAC AAATATTTAT ATCTGACCCC TCAGGATTAT 
AAGAGAGTCA GTGCTCTCAA CTCTGTCCAT TGTGAACATG TAGAGGACGA AGGAGAATCC 
AGGTACAAGA TAACTGATAT TATTGGGAAG GAAGAGGGAC TTGGAGCAGA GAACCTTCGA 
GGCTCTGGAA TGATCGCTGG AGAATCCTCA TTGGCCTATG ATGAGATCAT CACCATCAGC 
CTGGTTACAT GCCGGGCCAT TGGGATTGGA GCTTACCTTG TCCGGCTGGG ACAGAGAACC 
ATCCAGGTTG AAAATTCTCA TTTAATTTTG ACTGGAGCTG GAGCCCTCAA CAAAGTCCTT 
GGGCGGGAAG TATACACCTC CAATAACCAG CTCGGGGGCA TCCAGATCAT GCACAACAAT 
GGGGTGACCC ACAGCATTGT TTGTGATGAC TTTGAAGGGG TTTTCACTGT CCTGCACTGG 
CTATCCTACA TGCCTAAGAA TGTGCGCAGT TTAGTTCCTC TCCTGAACTC CAAGGATCCT 
ATAGATAGAA TCATCGAGTT TGTTCCCACG AAGGCCCCAT ACGATCCTCG ATGGATGCTA 
GCAGGCCGCC CTCACCCAAC TCAGAAAGGT CAGTGGCTGA GTGGATTTTT TGACTATGGC 
TCTTTCTCAG AGATCATGCA ACCCTGGGCA CAAACTGTGG TGGTTGGCAG AGCCAGGCTA 
GGAGGAATAC CTGTGGGAGT AGTTGCTGTA GAAACCCGAA CAGTGGAACT AAGTATCCCA 
GCCGATCCTG CAAACCTGGA TTCTGAAGCC AAGATAATCC AGCAGGCTGG CCAGGTTTGG 
TTCCCAGACT CTGCGTTTAA GACCTATCAG GCTATCAAGG ACTTCAACCG TGAAGGACTG 
CCTCTGATGG TCTTTGCCAA CTGGAGAGGC TTCTCTGGTG GGATGAAAGA TATGTATGAC 
CAAGTGCTGA AGTTTGGTGC CTATATTGTG GATAGCTTAC GGGAGTGCTC CCAGCCTGTG 
CTGGTATACA TTCCTCCTCA GGCTGAGCTC CGGGGTGGCT CCTGGGTTGT GATTGACCCC 
ACCATCAACC CCCGGCATAT GGAGATGTAC GCTGACCGAG AAAGCAGGGG ATCAGTTCTG 
GAGCCAGAAG GGACAGTAGA AATCAAATTC CGCAGAAAGG ATCTGGTGAA AACCATGCGT 
CGGGTGGACC CAGTCTACAT TCGCTTGGCT GAGCGATTGG GCACCCCAGA GCTCAGCGCA 
GCTGAGCGGA AGGAGCTGGA GAACAAGTTG AAGGAGCGGG AGGAATTCCT AATTCCCATT 
TACCATCAGG TAGCCGTGCA GTTTGCTGAC TTGCACGACA CACCAGGCCG GATGCAGGAG 
AAGGGTGTTA TTAATGACAT CCTGGATTGG AAAACATCCC GTACCTTTTT CTATTGGCGG 
CTGAGGCGTC TCCTGCTGGA GGACCTGGTC AAAAAGAAAA TCCACAATGC CAACCCAGAG 
CTGACCGATG GCCAGATCCA GGCCATGCTA CGGCGCTGGT TTGTAGAAGT GGAAGGAACT 
GTGAAGGCCT ACGTTTGGGA CAATAACAAG GACCTGGTGG AGTGGCTGGA GAAACAGCTG 
ACCGAGGAAG ATGGTGTTCG CTCAGTAATC GAGGAAAACA TCAAATATAT CAGCAGAGAC 
TATGTCCTCA AGCAGATCCG CAGCTTGGTA CAGGCCAATC CCGAGGTTGC CATGGACTCC 
ATCGTCCATA TGACTCAGCA TATCTCACCC ACTCAGCGAG CAGAAGTCGT ACGGATCCTC 
TCCACGATGG ACTCCCCTTC AACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_005624775.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624832.1 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X1, mRNA.

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CloneID	OCe12090
Clone ID Related Accession (Same CDS sequence)	XM_005624775.2 , XM_005624775.3
Accession Version	XM_005624775.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	acetyl-CoA carboxylase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624775.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGGT CCACTCTGAT GTCAATCTTG AGGGTTAGTT CTTTTTGGAG GTGGATATCT 
GCTCAGACAG TGAGAATTAT AAGAGCTTTG AGAGCTCATT TTGAAAGAAT AATGGATGAA 
CCATCCCCCT TGGCCAAACC TCTGGAGCTG AACCAGCACT CCCGATTCAT AATTGGTTCT 
GTGTCTGAAG ATAACTCAGA GGATGAAATT AGCAACCTGG TGAAGCTGGA TCTCCTGGAG 
GAGAAGGAGG GCTCTCTATC ACCAGCTTCT GTTGGCTCAG ATACACTTTC TGACTTGGGG 
ATCTCCAGCC TACAGGACGG CTTGGCCTTA CACATGAGGT CCAGCATGTC TGGCTTGCAT 
CTAGTGAAGC AAGGTCGAGA CCGAAAGAAA ATAGACTCTC AGCGAGATTT CACTGTAGCT 
TCTCCAGCAG AATTTGTTAC TCGTTTTGGG GGAAATAAAG TGATTGAGAA GGTTCTTATT 
GCCAACAATG GCATTGCAGC AGTGAAATGC ATGCGGTCTA TCCGTCGATG GTCTTATGAG 
ATGTTTCGAA ATGAACGTGC AATCCGATTT GTTGTTATGG TAACACCTGA AGACCTGAAA 
GCCAATGCAG AATACATTAA GATGGCGGAT CACTATGTGC CAGTGCCAGG AGGACCAAAT 
AACAACAACT ATGCGAACGT GGAATTAATT CTTGATATTG CTAAAAGGAT TCCCGTACAA 
GCAGTGTGGG CTGGCTGGGG TCATGCTTCT GAGAATCCCA AGCTCCCAGA ACTTCTTTTG 
AAAAATGGCA TCGCCTTCAT GGGCCCTCCA AGCCAGGCCA TGTGGGCTTT GGGGGATAAG 
ATTGCATCTT CTATAGTGGC TCAAACTGCA GGTATCCCAA CTCTTCCCTG GAGTGGCAGT 
GGTCTTCGGG TGGACTGGCA TGAAAATGAT TTTTCCAAAC GTATCTTAAA TGTTCCCCAG 
GAATTATACG AAAAAGGTTA TGTGAAGGAT GTGGATGATG GGCTAAAGGC AGCGGAGGAA 
GTTGGATATC CAGTAATGAT CAAGGCCTCA GAAGGAGGAG GAGGGAAAGG AATCAGAAAA 
GTCAACAATG CAGATGATTT CCCTAACCTC TTCAGACAGG TTCAAGCTGA AGTCCCTGGA 
TCTCCTATAT TTGTGATGAG ACTAGCCAAA CAATCTCGTC ATCTGGAGGT ACAGATCTTA 
GCAGATCAAT ATGGCAATGC TATCTCTCTC TTTGGTCGTG ACTGCTCTGT ACAGCGCAGG 
CATCAGAAGA TTATTGAGGA AGCACCTGCT GCTATTGCTA CTCCAGCAGT ATTTGAACAT 
ATGGAACAGT GTGCTGTGAA ACTTGCCAAA ATGGTTGGTT ATGTGAGTGC TGGGACTGTG 
GAATACCTCT ACAGCCAGGA TGGAAGCTTC TACTTTCTGG AACTGAACCC TCGGCTACAG 
GTGGAACACC CTTGTACAGA GATGGTGGCT GATGTCAACC TCCCTGCAGC ACAACTCCAG 
ATTGCCATGG GGATCCCTCT GTACAGAATC AAGGATATTC GTATGATGTA TGGGGTATCT 
CCCTGGGGTG ATGCTCCCAT TGATTTTGAA AATTCTGCTC ACGTTCCTTG CCCAAGGGGC 
CATGTTATTG CCGCTCGGAT CACTAGTGAA AATCCAGATG AGGGTTTTAA GCCCAGCTCA 
GGAACAGTTC AGGAACTGAA TTTCCGTAGC AATAAGAATG TTTGGGGTTA TTTCAGTGTT 
GCTGCTGCAG GGGGCCTTCA TGAATTTGCT GATTCTCAGT TTGGTCATTG CTTCTCCTGG 
GGAGAAAACA GAGAGGAAGC AATTTCAAAC ATGGTGGTGG CTTTGAAGGA GCTGTCTATT 
CGGGGTGACT TTCGAACCAC AGTTGAATAC CTCATCAAAT TGTTGGAGAC TGAAAGCTTT 
CAGTTGAATA GAATTGACAC CGGCTGGCTG GACAGACTGA TAGCAGAGAA AGTACAGGCA 
GAGCGACCTG ACACCATGTT GGGAGTTGTT TGTGGGGCTC TCCATGTGGC AGATGTGAGC 
CTACGGAATA GCATCTCTAA CTTCCTTCAT TCTTTAGAAA GGGGTCAAGT CCTTCCTGCT 
CATACACTTC TGAATACAGT AGATGTTGAA CTTATCTATG GGGGAGTCAA ATATGTACTT 
AAGGTGACTC GGCAGTCCCC CAACTCCTAT GTGGTGATCA TGAATGGTTC ATGCGTAGAA 
GTAGATGTGC ATCGGCTCAG TGATGGAGGG TTACTCCTGT CCTATGATGG CAGCAGTTAC 
ACCACATACA TGAAGGAAGA AGTGGATAGA TATCGTATCA CAATTGGCAA TAAGACCTGT 
GTGTTTGAGA AGGAAAATGA CCCTTCAGTG TTACGCTCAC CTTCTGCTGG GAAGTTAATC 
CAGTACATTG TGGAGGATGG AGGCCATGTG TTTGCTGGCC AGTGCTATGC TGAAATTGAG 
GTGATGAAGA TGGTAATGAC CTTAACAGCT GCAGAGTCTG GCTGTATCCA TTATGTCAAA 
CGGCCTGGAG CAGCTCTTGA CCCTGGCTGT GTAATAGCCA AAATGCAGTT GGACAACCCC 
AGCAAGGTCC AACAGGCTGA GCTTCACACA GGTAGTCTGC CACGGATCCA GAGCACTGCA 
CTCAGAGGCG AGAAGCTCCA TCGAGTGTTC CACTATGTCC TGGATAATCT GGTCAACGTA 
ATGAATGGAT ACTGCCTTCC AGATCCTTTT TTTAGCAGCA GGGTAAAAGA CTGGGTAGAG 
CGGTTGATGA AGACCCTCAG AGACCCCTCG TTACCTCTCC TAGAATTACA AGATATCATG 
ACCAGTGTCT CTGGCCGTAT TCCCCCCAAT GTTGAGAAGT CTATCAAGAA GGAAATGGCC 
CAGTATGCCA GCAATATCAC ATCAGTCCTC TGTCAGTTTC CCAGCCAGCA GATTGCCAAC 
ATCCTAGATA GCCATGCAGC TACGTTGAAC CGGAAATCTG AGCGGGAAGT CTTTTTCATG 
AACACTCAGA GCATTGTCCA GCTGGTACAG AGATACCGAA GTGGCATCCG AGGCCACATG 
AAGGCTGTGG TGATGGATCT GCTGCGGCAG TACCTGCGAG TAGAGACACA GTTCCAGAAT 
GGTCACTATG ACAAATGTGT GTTTGCCCTT CGAGAAGAGA ACAAGAGTGA TATGAACACT 
GTACTGAACT ATATCTTCTC TCATGCTCAA GTCACCAAGA AGAATCTTCT GGTCACAATG 
CTTATTGATC AGTTGTGCGG TAGGGACCCT ACTCTCACTG ATGAGCTGCT GAATATTCTC 
ACAGAGCTAA CTCAACTCAG TAAGACCACC AATGCCAAAG TAGCACTTCG AGCACGCCAG 
GTTCTTATTG CCTCCCATTT GCCATCATAT GAGCTTCGCC ATAACCAAGT AGAGTCTATC 
TTCCTTTCAG CTATTGACAT GTATGGACAT CAGTTTTGCA TTGAGAACCT GCAGAAACTC 
ATCTTGTCTG AAACATCTAT TTTTGATGTC CTACCAAACT TCTTCTATCA CAGCAACCAG 
GTAGTGAGGA TGGCAGCTCT GGAGGTTTAT GTTCGAAGGG CTTATATTGC CTATGAACTT 
AACAGCGTAC AACATCGCCA ACTTAAGGAC AACACCTGTG TGGTGGAATT CCAATTCATG 
CTACCCACAT CTCATCCAAA TAGAGGGAAC ATCCCCACGC TAAACAGAAT GTCCTTCTCC 
TCCAACCTCA ACCACTATGG CATGACTCAT GTAGCTAGTG TCAGCGATGT GCTTTTGGAC 
AACTCATTCA CTCCACCATG TCAACGGATG GGCGGAATGG TCTCTTTTCG GACTTTCGAA 
GATTTTGTCA GGATCTTTGA TGAAGTGATG GGCTGCTTCT GTGATTCTCC ACCGCAAAGC 
CCTACATTCC CTGAGGCAGG TCACACATCT CTATACGATG AAGACAAGGT CCCCAGGGAT 
GAACCAATTC ACATTCTGAA TGTGGCTATC AAGACTGACT GTGATATTGA GGATGACAGA 
CTGGCAGCTA TGTTCAGAGA GTTTACCCAA CAAAATAAAG CTACCCTGGT TGAGCATGGG 
ATACGGCGCC TTACTTTCCT GGTTGCACAA AAGGATTTCA GGAAACAGGT CAACTATGAG 
GTGGATCAGA GATTTCATAG AGAATTTCCT AAATTTTTCA CCTTCCGAGC AAGGGATAAG 
TTTGAGGAGG ATCGTATCTA TCGTCACCTG GAACCTGCCC TCGCTTTCCA GTTAGAGCTG 
AACCGGATGA GAAATTTTGA CCTTACTGCC ATTCCATGTG CTAATCATAA AATGCATCTG 
TATCTTGGGG CAGCCAAGGT GGAGGTGGGC ACAGAAGTGA CAGACTACAG GTTCTTTGTT 
CGTGCAATCA TCAGACATTC TGATCTGGTC ACCAAGGAAG CTTCTTTTGA ATACCTACAA 
AATGAAGGGG AGAGGCTGCT TCTAGAAGCC ATGGATGAGT TGGAAGTTGC TTTTAACAAT 
ACAAACGTTC GCACTGACTG TAACCACATC TTCCTCAACT TTGTGCCCAC GGTCATCATG 
GACCCATCAA AGATTGAGGA ATCTGTGCGG AGCATGGTAA TGCGCTATGG AAGTCGGCTG 
TGGAAATTAC GTGTCCTCCA GGCAGAACTG AAAATCAACA TTCGCCTGAC ACCAACTGGA 
AAAGCAATTC CCATCCGCCT CTTCCTGACA AACGAGTCTG GCTATTACTT GGACATCAGC 
TTGTATAAGG AAGTGACTGA CTCCAGGACA GCACAGATCA TGTTTCAGGC ATATGGAGAC 
AAACAGGGAC CATTGCATGG AATGTTAATC AACACTCCAT ACGTCACCAA AGACCTGCTT 
CAATCAAAGA GATTCCAGGC ACAATCCTTG GGGACAACAT ACATATATGA TATACCAGAG 
ATGTTTCGGC AGTCTCTGAT CAAACTCTGG GAGTCTATGT CCACTCAAGC ATTTCTTCCA 
TCACCCCCCC TGCCTTCCGA CATGCTGACA TATACGGAGC TCGTGTTGGA TGATCAAGGT 
CAGCTGGTCC ACATGAACAG GCTTCCAGGA GGAAATGAGA TTGGCATGGT AGCGTGGAAA 
ATGACCCTTA AAAGTCCTGA ATACCCAGAA GGCCGAGATA TCATTGTTAT TGGCAATGAC 
ATCACATACC GAATTGGGTC CTTTGGGCCC CAGGAGGATT TGCTGTTTCT CAGAGCTTCT 
GAGCTTGCCA GGGCAGAGGG TATCCCACGC ATCTTTGTAG CAGCCAACAG TGGAGCAAGA 
ATTGGACTGG CAGAGGAAAT TCGTCATATG TTTCATGTAG CCTGGGTAGA TCCTGAGGAT 
CCTTACAAGG GATACAAATA TTTATATCTG ACCCCTCAGG ATTATAAGAG AGTCAGTGCT 
CTCAACTCTG TCCATTGTGA ACATGTAGAG GACGAAGGAG AATCCAGGTA CAAGATAACT 
GATATTATTG GGAAGGAAGA GGGACTTGGA GCAGAGAACC TTCGAGGCTC TGGAATGATC 
GCTGGAGAAT CCTCATTGGC CTATGATGAG ATCATCACCA TCAGCCTGGT TACATGCCGG 
GCCATTGGGA TTGGAGCTTA CCTTGTCCGG CTGGGACAGA GAACCATCCA GGTTGAAAAT 
TCTCATTTAA TTTTGACTGG AGCTGGAGCC CTCAACAAAG TCCTTGGGCG GGAAGTATAC 
ACCTCCAATA ACCAGCTCGG GGGCATCCAG ATCATGCACA ACAATGGGGT GACCCACAGC 
ATTGTTTGTG ATGACTTTGA AGGGGTTTTC ACTGTCCTGC ACTGGCTATC CTACATGCCT 
AAGAATGTGC GCAGTTTAGT TCCTCTCCTG AACTCCAAGG ATCCTATAGA TAGAATCATC 
GAGTTTGTTC CCACGAAGGC CCCATACGAT CCTCGATGGA TGCTAGCAGG CCGCCCTCAC 
CCAACTCAGA AAGGTCAGTG GCTGAGTGGA TTTTTTGACT ATGGCTCTTT CTCAGAGATC 
ATGCAACCCT GGGCACAAAC TGTGGTGGTT GGCAGAGCCA GGCTAGGAGG AATACCTGTG 
GGAGTAGTTG CTGTAGAAAC CCGAACAGTG GAACTAAGTA TCCCAGCCGA TCCTGCAAAC 
CTGGATTCTG AAGCCAAGAT AATCCAGCAG GCTGGCCAGG TTTGGTTCCC AGACTCTGCG 
TTTAAGACCT ATCAGGCTAT CAAGGACTTC AACCGTGAAG GACTGCCTCT GATGGTCTTT 
GCCAACTGGA GAGGCTTCTC TGGTGGGATG AAAGATATGT ATGACCAAGT GCTGAAGTTT 
GGTGCCTATA TTGTGGATAG CTTACGGGAG TGCTCCCAGC CTGTGCTGGT ATACATTCCT 
CCTCAGGCTG AGCTCCGGGG TGGCTCCTGG GTTGTGATTG ACCCCACCAT CAACCCCCGG 
CATATGGAGA TGTACGCTGA CCGAGAAAGC AGGGGATCAG TTCTGGAGCC AGAAGGGACA 
GTAGAAATCA AATTCCGCAG AAAGGATCTG GTGAAAACCA TGCGTCGGGT GGACCCAGTC 
TACATTCGCT TGGCTGAGCG ATTGGGCACC CCAGAGCTCA GCGCAGCTGA GCGGAAGGAG 
CTGGAGAACA AGTTGAAGGA GCGGGAGGAA TTCCTAATTC CCATTTACCA TCAGGTAGCC 
GTGCAGTTTG CTGACTTGCA CGACACACCA GGCCGGATGC AGGAGAAGGG TGTTATTAAT 
GACATCCTGG ATTGGAAAAC ATCCCGTACC TTTTTCTATT GGCGGCTGAG GCGTCTCCTG 
CTGGAGGACC TGGTCAAAAA GAAAATCCAC AATGCCAACC CAGAGCTGAC CGATGGCCAG 
ATCCAGGCCA TGCTACGGCG CTGGTTTGTA GAAGTGGAAG GAACTGTGAA GGCCTACGTT 
TGGGACAATA ACAAGGACCT GGTGGAGTGG CTGGAGAAAC AGCTGACCGA GGAAGATGGT 
GTTCGCTCAG TAATCGAGGA AAACATCAAA TATATCAGCA GAGACTATGT CCTCAAGCAG 
ATCCGCAGCT TGGTACAGGC CAATCCCGAG GTTGCCATGG ACTCCATCGT CCATATGACT 
CAGCATATCT CACCCACTCA GCGAGCAGAA GTCGTACGGA TCCTCTCCAC GATGGACTCC 
CCTTCAACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624832.1
CDS	312..7463
Translation	MWWSTLMSILRVSSFWRWISAQTVRIIRALRAHFERIMDEPSPLAKPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_005624775.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624775.2

ORF Insert Sequence:

ATGTGGTGGT CCACTCTGAT GTCAATCTTG AGGGTTAGTT CTTTTTGGAG GTGGATATCT 
GCTCAGACAG TGAGAATTAT AAGAGCTTTG AGAGCTCATT TTGAAAGAAT AATGGATGAA 
CCATCCCCCT TGGCCAAACC TCTGGAGCTG AACCAGCACT CCCGATTCAT AATTGGTTCT 
GTGTCTGAAG ATAACTCAGA GGATGAAATT AGCAACCTGG TGAAGCTGGA TCTCCTGGAG 
GAGAAGGAGG GCTCTCTATC ACCAGCTTCT GTTGGCTCAG ATACACTTTC TGACTTGGGG 
ATCTCCAGCC TACAGGACGG CTTGGCCTTA CACATGAGGT CCAGCATGTC TGGCTTGCAT 
CTAGTGAAGC AAGGTCGAGA CCGAAAGAAA ATAGACTCTC AGCGAGATTT CACTGTAGCT 
TCTCCAGCAG AATTTGTTAC TCGTTTTGGG GGAAATAAAG TGATTGAGAA GGTTCTTATT 
GCCAACAATG GCATTGCAGC AGTGAAATGC ATGCGGTCTA TCCGTCGATG GTCTTATGAG 
ATGTTTCGAA ATGAACGTGC AATCCGATTT GTTGTTATGG TAACACCTGA AGACCTGAAA 
GCCAATGCAG AATACATTAA GATGGCGGAT CACTATGTGC CAGTGCCAGG AGGACCAAAT 
AACAACAACT ATGCGAACGT GGAATTAATT CTTGATATTG CTAAAAGGAT TCCCGTACAA 
GCAGTGTGGG CTGGCTGGGG TCATGCTTCT GAGAATCCCA AGCTCCCAGA ACTTCTTTTG 
AAAAATGGCA TCGCCTTCAT GGGCCCTCCA AGCCAGGCCA TGTGGGCTTT GGGGGATAAG 
ATTGCATCTT CTATAGTGGC TCAAACTGCA GGTATCCCAA CTCTTCCCTG GAGTGGCAGT 
GGTCTTCGGG TGGACTGGCA TGAAAATGAT TTTTCCAAAC GTATCTTAAA TGTTCCCCAG 
GAATTATACG AAAAAGGTTA TGTGAAGGAT GTGGATGATG GGCTAAAGGC AGCGGAGGAA 
GTTGGATATC CAGTAATGAT CAAGGCCTCA GAAGGAGGAG GAGGGAAAGG AATCAGAAAA 
GTCAACAATG CAGATGATTT CCCTAACCTC TTCAGACAGG TTCAAGCTGA AGTCCCTGGA 
TCTCCTATAT TTGTGATGAG ACTAGCCAAA CAATCTCGTC ATCTGGAGGT ACAGATCTTA 
GCAGATCAAT ATGGCAATGC TATCTCTCTC TTTGGTCGTG ACTGCTCTGT ACAGCGCAGG 
CATCAGAAGA TTATTGAGGA AGCACCTGCT GCTATTGCTA CTCCAGCAGT ATTTGAACAT 
ATGGAACAGT GTGCTGTGAA ACTTGCCAAA ATGGTTGGTT ATGTGAGTGC TGGGACTGTG 
GAATACCTCT ACAGCCAGGA TGGAAGCTTC TACTTTCTGG AACTGAACCC TCGGCTACAG 
GTGGAACACC CTTGTACAGA GATGGTGGCT GATGTCAACC TCCCTGCAGC ACAACTCCAG 
ATTGCCATGG GGATCCCTCT GTACAGAATC AAGGATATTC GTATGATGTA TGGGGTATCT 
CCCTGGGGTG ATGCTCCCAT TGATTTTGAA AATTCTGCTC ACGTTCCTTG CCCAAGGGGC 
CATGTTATTG CCGCTCGGAT CACTAGTGAA AATCCAGATG AGGGTTTTAA GCCCAGCTCA 
GGAACAGTTC AGGAACTGAA TTTCCGTAGC AATAAGAATG TTTGGGGTTA TTTCAGTGTT 
GCTGCTGCAG GGGGCCTTCA TGAATTTGCT GATTCTCAGT TTGGTCATTG CTTCTCCTGG 
GGAGAAAACA GAGAGGAAGC AATTTCAAAC ATGGTGGTGG CTTTGAAGGA GCTGTCTATT 
CGGGGTGACT TTCGAACCAC AGTTGAATAC CTCATCAAAT TGTTGGAGAC TGAAAGCTTT 
CAGTTGAATA GAATTGACAC CGGCTGGCTG GACAGACTGA TAGCAGAGAA AGTACAGGCA 
GAGCGACCTG ACACCATGTT GGGAGTTGTT TGTGGGGCTC TCCATGTGGC AGATGTGAGC 
CTACGGAATA GCATCTCTAA CTTCCTTCAT TCTTTAGAAA GGGGTCAAGT CCTTCCTGCT 
CATACACTTC TGAATACAGT AGATGTTGAA CTTATCTATG GGGGAGTCAA ATATGTACTT 
AAGGTGACTC GGCAGTCCCC CAACTCCTAT GTGGTGATCA TGAATGGTTC ATGCGTAGAA 
GTAGATGTGC ATCGGCTCAG TGATGGAGGG TTACTCCTGT CCTATGATGG CAGCAGTTAC 
ACCACATACA TGAAGGAAGA AGTGGATAGA TATCGTATCA CAATTGGCAA TAAGACCTGT 
GTGTTTGAGA AGGAAAATGA CCCTTCAGTG TTACGCTCAC CTTCTGCTGG GAAGTTAATC 
CAGTACATTG TGGAGGATGG AGGCCATGTG TTTGCTGGCC AGTGCTATGC TGAAATTGAG 
GTGATGAAGA TGGTAATGAC CTTAACAGCT GCAGAGTCTG GCTGTATCCA TTATGTCAAA 
CGGCCTGGAG CAGCTCTTGA CCCTGGCTGT GTAATAGCCA AAATGCAGTT GGACAACCCC 
AGCAAGGTCC AACAGGCTGA GCTTCACACA GGTAGTCTGC CACGGATCCA GAGCACTGCA 
CTCAGAGGCG AGAAGCTCCA TCGAGTGTTC CACTATGTCC TGGATAATCT GGTCAACGTA 
ATGAATGGAT ACTGCCTTCC AGATCCTTTT TTTAGCAGCA GGGTAAAAGA CTGGGTAGAG 
CGGTTGATGA AGACCCTCAG AGACCCCTCG TTACCTCTCC TAGAATTACA AGATATCATG 
ACCAGTGTCT CTGGCCGTAT TCCCCCCAAT GTTGAGAAGT CTATCAAGAA GGAAATGGCC 
CAGTATGCCA GCAATATCAC ATCAGTCCTC TGTCAGTTTC CCAGCCAGCA GATTGCCAAC 
ATCCTAGATA GCCATGCAGC TACGTTGAAC CGGAAATCTG AGCGGGAAGT CTTTTTCATG 
AACACTCAGA GCATTGTCCA GCTGGTACAG AGATACCGAA GTGGCATCCG AGGCCACATG 
AAGGCTGTGG TGATGGATCT GCTGCGGCAG TACCTGCGAG TAGAGACACA GTTCCAGAAT 
GGTCACTATG ACAAATGTGT GTTTGCCCTT CGAGAAGAGA ACAAGAGTGA TATGAACACT 
GTACTGAACT ATATCTTCTC TCATGCTCAA GTCACCAAGA AGAATCTTCT GGTCACAATG 
CTTATTGATC AGTTGTGCGG TAGGGACCCT ACTCTCACTG ATGAGCTGCT GAATATTCTC 
ACAGAGCTAA CTCAACTCAG TAAGACCACC AATGCCAAAG TAGCACTTCG AGCACGCCAG 
GTTCTTATTG CCTCCCATTT GCCATCATAT GAGCTTCGCC ATAACCAAGT AGAGTCTATC 
TTCCTTTCAG CTATTGACAT GTATGGACAT CAGTTTTGCA TTGAGAACCT GCAGAAACTC 
ATCTTGTCTG AAACATCTAT TTTTGATGTC CTACCAAACT TCTTCTATCA CAGCAACCAG 
GTAGTGAGGA TGGCAGCTCT GGAGGTTTAT GTTCGAAGGG CTTATATTGC CTATGAACTT 
AACAGCGTAC AACATCGCCA ACTTAAGGAC AACACCTGTG TGGTGGAATT CCAATTCATG 
CTACCCACAT CTCATCCAAA TAGAGGGAAC ATCCCCACGC TAAACAGAAT GTCCTTCTCC 
TCCAACCTCA ACCACTATGG CATGACTCAT GTAGCTAGTG TCAGCGATGT GCTTTTGGAC 
AACTCATTCA CTCCACCATG TCAACGGATG GGCGGAATGG TCTCTTTTCG GACTTTCGAA 
GATTTTGTCA GGATCTTTGA TGAAGTGATG GGCTGCTTCT GTGATTCTCC ACCGCAAAGC 
CCTACATTCC CTGAGGCAGG TCACACATCT CTATACGATG AAGACAAGGT CCCCAGGGAT 
GAACCAATTC ACATTCTGAA TGTGGCTATC AAGACTGACT GTGATATTGA GGATGACAGA 
CTGGCAGCTA TGTTCAGAGA GTTTACCCAA CAAAATAAAG CTACCCTGGT TGAGCATGGG 
ATACGGCGCC TTACTTTCCT GGTTGCACAA AAGGATTTCA GGAAACAGGT CAACTATGAG 
GTGGATCAGA GATTTCATAG AGAATTTCCT AAATTTTTCA CCTTCCGAGC AAGGGATAAG 
TTTGAGGAGG ATCGTATCTA TCGTCACCTG GAACCTGCCC TCGCTTTCCA GTTAGAGCTG 
AACCGGATGA GAAATTTTGA CCTTACTGCC ATTCCATGTG CTAATCATAA AATGCATCTG 
TATCTTGGGG CAGCCAAGGT GGAGGTGGGC ACAGAAGTGA CAGACTACAG GTTCTTTGTT 
CGTGCAATCA TCAGACATTC TGATCTGGTC ACCAAGGAAG CTTCTTTTGA ATACCTACAA 
AATGAAGGGG AGAGGCTGCT TCTAGAAGCC ATGGATGAGT TGGAAGTTGC TTTTAACAAT 
ACAAACGTTC GCACTGACTG TAACCACATC TTCCTCAACT TTGTGCCCAC GGTCATCATG 
GACCCATCAA AGATTGAGGA ATCTGTGCGG AGCATGGTAA TGCGCTATGG AAGTCGGCTG 
TGGAAATTAC GTGTCCTCCA GGCAGAACTG AAAATCAACA TTCGCCTGAC ACCAACTGGA 
AAAGCAATTC CCATCCGCCT CTTCCTGACA AACGAGTCTG GCTATTACTT GGACATCAGC 
TTGTATAAGG AAGTGACTGA CTCCAGGACA GCACAGATCA TGTTTCAGGC ATATGGAGAC 
AAACAGGGAC CATTGCATGG AATGTTAATC AACACTCCAT ACGTCACCAA AGACCTGCTT 
CAATCAAAGA GATTCCAGGC ACAATCCTTG GGGACAACAT ACATATATGA TATACCAGAG 
ATGTTTCGGC AGTCTCTGAT CAAACTCTGG GAGTCTATGT CCACTCAAGC ATTTCTTCCA 
TCACCCCCCC TGCCTTCCGA CATGCTGACA TATACGGAGC TCGTGTTGGA TGATCAAGGT 
CAGCTGGTCC ACATGAACAG GCTTCCAGGA GGAAATGAGA TTGGCATGGT AGCGTGGAAA 
ATGACCCTTA AAAGTCCTGA ATACCCAGAA GGCCGAGATA TCATTGTTAT TGGCAATGAC 
ATCACATACC GAATTGGGTC CTTTGGGCCC CAGGAGGATT TGCTGTTTCT CAGAGCTTCT 
GAGCTTGCCA GGGCAGAGGG TATCCCACGC ATCTTTGTAG CAGCCAACAG TGGAGCAAGA 
ATTGGACTGG CAGAGGAAAT TCGTCATATG TTTCATGTAG CCTGGGTAGA TCCTGAGGAT 
CCTTACAAGG GATACAAATA TTTATATCTG ACCCCTCAGG ATTATAAGAG AGTCAGTGCT 
CTCAACTCTG TCCATTGTGA ACATGTAGAG GACGAAGGAG AATCCAGGTA CAAGATAACT 
GATATTATTG GGAAGGAAGA GGGACTTGGA GCAGAGAACC TTCGAGGCTC TGGAATGATC 
GCTGGAGAAT CCTCATTGGC CTATGATGAG ATCATCACCA TCAGCCTGGT TACATGCCGG 
GCCATTGGGA TTGGAGCTTA CCTTGTCCGG CTGGGACAGA GAACCATCCA GGTTGAAAAT 
TCTCATTTAA TTTTGACTGG AGCTGGAGCC CTCAACAAAG TCCTTGGGCG GGAAGTATAC 
ACCTCCAATA ACCAGCTCGG GGGCATCCAG ATCATGCACA ACAATGGGGT GACCCACAGC 
ATTGTTTGTG ATGACTTTGA AGGGGTTTTC ACTGTCCTGC ACTGGCTATC CTACATGCCT 
AAGAATGTGC GCAGTTTAGT TCCTCTCCTG AACTCCAAGG ATCCTATAGA TAGAATCATC 
GAGTTTGTTC CCACGAAGGC CCCATACGAT CCTCGATGGA TGCTAGCAGG CCGCCCTCAC 
CCAACTCAGA AAGGTCAGTG GCTGAGTGGA TTTTTTGACT ATGGCTCTTT CTCAGAGATC 
ATGCAACCCT GGGCACAAAC TGTGGTGGTT GGCAGAGCCA GGCTAGGAGG AATACCTGTG 
GGAGTAGTTG CTGTAGAAAC CCGAACAGTG GAACTAAGTA TCCCAGCCGA TCCTGCAAAC 
CTGGATTCTG AAGCCAAGAT AATCCAGCAG GCTGGCCAGG TTTGGTTCCC AGACTCTGCG 
TTTAAGACCT ATCAGGCTAT CAAGGACTTC AACCGTGAAG GACTGCCTCT GATGGTCTTT 
GCCAACTGGA GAGGCTTCTC TGGTGGGATG AAAGATATGT ATGACCAAGT GCTGAAGTTT 
GGTGCCTATA TTGTGGATAG CTTACGGGAG TGCTCCCAGC CTGTGCTGGT ATACATTCCT 
CCTCAGGCTG AGCTCCGGGG TGGCTCCTGG GTTGTGATTG ACCCCACCAT CAACCCCCGG 
CATATGGAGA TGTACGCTGA CCGAGAAAGC AGGGGATCAG TTCTGGAGCC AGAAGGGACA 
GTAGAAATCA AATTCCGCAG AAAGGATCTG GTGAAAACCA TGCGTCGGGT GGACCCAGTC 
TACATTCGCT TGGCTGAGCG ATTGGGCACC CCAGAGCTCA GCGCAGCTGA GCGGAAGGAG 
CTGGAGAACA AGTTGAAGGA GCGGGAGGAA TTCCTAATTC CCATTTACCA TCAGGTAGCC 
GTGCAGTTTG CTGACTTGCA CGACACACCA GGCCGGATGC AGGAGAAGGG TGTTATTAAT 
GACATCCTGG ATTGGAAAAC ATCCCGTACC TTTTTCTATT GGCGGCTGAG GCGTCTCCTG 
CTGGAGGACC TGGTCAAAAA GAAAATCCAC AATGCCAACC CAGAGCTGAC CGATGGCCAG 
ATCCAGGCCA TGCTACGGCG CTGGTTTGTA GAAGTGGAAG GAACTGTGAA GGCCTACGTT 
TGGGACAATA ACAAGGACCT GGTGGAGTGG CTGGAGAAAC AGCTGACCGA GGAAGATGGT 
GTTCGCTCAG TAATCGAGGA AAACATCAAA TATATCAGCA GAGACTATGT CCTCAAGCAG 
ATCCGCAGCT TGGTACAGGC CAATCCCGAG GTTGCCATGG ACTCCATCGT CCATATGACT 
CAGCATATCT CACCCACTCA GCGAGCAGAA GTCGTACGGA TCCTCTCCAC GATGGACTCC 
CCTTCAACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_005624775.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624832.1 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X1, mRNA.

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CloneID	OCe12090
Clone ID Related Accession (Same CDS sequence)	XM_005624775.2 , XM_005624775.3
Accession Version	XM_005624775.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	acetyl-CoA carboxylase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624775.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGGT CCACTCTGAT GTCAATCTTG AGGGTTAGTT CTTTTTGGAG GTGGATATCT 
GCTCAGACAG TGAGAATTAT AAGAGCTTTG AGAGCTCATT TTGAAAGAAT AATGGATGAA 
CCATCCCCCT TGGCCAAACC TCTGGAGCTG AACCAGCACT CCCGATTCAT AATTGGTTCT 
GTGTCTGAAG ATAACTCAGA GGATGAAATT AGCAACCTGG TGAAGCTGGA TCTCCTGGAG 
GAGAAGGAGG GCTCTCTATC ACCAGCTTCT GTTGGCTCAG ATACACTTTC TGACTTGGGG 
ATCTCCAGCC TACAGGACGG CTTGGCCTTA CACATGAGGT CCAGCATGTC TGGCTTGCAT 
CTAGTGAAGC AAGGTCGAGA CCGAAAGAAA ATAGACTCTC AGCGAGATTT CACTGTAGCT 
TCTCCAGCAG AATTTGTTAC TCGTTTTGGG GGAAATAAAG TGATTGAGAA GGTTCTTATT 
GCCAACAATG GCATTGCAGC AGTGAAATGC ATGCGGTCTA TCCGTCGATG GTCTTATGAG 
ATGTTTCGAA ATGAACGTGC AATCCGATTT GTTGTTATGG TAACACCTGA AGACCTGAAA 
GCCAATGCAG AATACATTAA GATGGCGGAT CACTATGTGC CAGTGCCAGG AGGACCAAAT 
AACAACAACT ATGCGAACGT GGAATTAATT CTTGATATTG CTAAAAGGAT TCCCGTACAA 
GCAGTGTGGG CTGGCTGGGG TCATGCTTCT GAGAATCCCA AGCTCCCAGA ACTTCTTTTG 
AAAAATGGCA TCGCCTTCAT GGGCCCTCCA AGCCAGGCCA TGTGGGCTTT GGGGGATAAG 
ATTGCATCTT CTATAGTGGC TCAAACTGCA GGTATCCCAA CTCTTCCCTG GAGTGGCAGT 
GGTCTTCGGG TGGACTGGCA TGAAAATGAT TTTTCCAAAC GTATCTTAAA TGTTCCCCAG 
GAATTATACG AAAAAGGTTA TGTGAAGGAT GTGGATGATG GGCTAAAGGC AGCGGAGGAA 
GTTGGATATC CAGTAATGAT CAAGGCCTCA GAAGGAGGAG GAGGGAAAGG AATCAGAAAA 
GTCAACAATG CAGATGATTT CCCTAACCTC TTCAGACAGG TTCAAGCTGA AGTCCCTGGA 
TCTCCTATAT TTGTGATGAG ACTAGCCAAA CAATCTCGTC ATCTGGAGGT ACAGATCTTA 
GCAGATCAAT ATGGCAATGC TATCTCTCTC TTTGGTCGTG ACTGCTCTGT ACAGCGCAGG 
CATCAGAAGA TTATTGAGGA AGCACCTGCT GCTATTGCTA CTCCAGCAGT ATTTGAACAT 
ATGGAACAGT GTGCTGTGAA ACTTGCCAAA ATGGTTGGTT ATGTGAGTGC TGGGACTGTG 
GAATACCTCT ACAGCCAGGA TGGAAGCTTC TACTTTCTGG AACTGAACCC TCGGCTACAG 
GTGGAACACC CTTGTACAGA GATGGTGGCT GATGTCAACC TCCCTGCAGC ACAACTCCAG 
ATTGCCATGG GGATCCCTCT GTACAGAATC AAGGATATTC GTATGATGTA TGGGGTATCT 
CCCTGGGGTG ATGCTCCCAT TGATTTTGAA AATTCTGCTC ACGTTCCTTG CCCAAGGGGC 
CATGTTATTG CCGCTCGGAT CACTAGTGAA AATCCAGATG AGGGTTTTAA GCCCAGCTCA 
GGAACAGTTC AGGAACTGAA TTTCCGTAGC AATAAGAATG TTTGGGGTTA TTTCAGTGTT 
GCTGCTGCAG GGGGCCTTCA TGAATTTGCT GATTCTCAGT TTGGTCATTG CTTCTCCTGG 
GGAGAAAACA GAGAGGAAGC AATTTCAAAC ATGGTGGTGG CTTTGAAGGA GCTGTCTATT 
CGGGGTGACT TTCGAACCAC AGTTGAATAC CTCATCAAAT TGTTGGAGAC TGAAAGCTTT 
CAGTTGAATA GAATTGACAC CGGCTGGCTG GACAGACTGA TAGCAGAGAA AGTACAGGCA 
GAGCGACCTG ACACCATGTT GGGAGTTGTT TGTGGGGCTC TCCATGTGGC AGATGTGAGC 
CTACGGAATA GCATCTCTAA CTTCCTTCAT TCTTTAGAAA GGGGTCAAGT CCTTCCTGCT 
CATACACTTC TGAATACAGT AGATGTTGAA CTTATCTATG GGGGAGTCAA ATATGTACTT 
AAGGTGACTC GGCAGTCCCC CAACTCCTAT GTGGTGATCA TGAATGGTTC ATGCGTAGAA 
GTAGATGTGC ATCGGCTCAG TGATGGAGGG TTACTCCTGT CCTATGATGG CAGCAGTTAC 
ACCACATACA TGAAGGAAGA AGTGGATAGA TATCGTATCA CAATTGGCAA TAAGACCTGT 
GTGTTTGAGA AGGAAAATGA CCCTTCAGTG TTACGCTCAC CTTCTGCTGG GAAGTTAATC 
CAGTACATTG TGGAGGATGG AGGCCATGTG TTTGCTGGCC AGTGCTATGC TGAAATTGAG 
GTGATGAAGA TGGTAATGAC CTTAACAGCT GCAGAGTCTG GCTGTATCCA TTATGTCAAA 
CGGCCTGGAG CAGCTCTTGA CCCTGGCTGT GTAATAGCCA AAATGCAGTT GGACAACCCC 
AGCAAGGTCC AACAGGCTGA GCTTCACACA GGTAGTCTGC CACGGATCCA GAGCACTGCA 
CTCAGAGGCG AGAAGCTCCA TCGAGTGTTC CACTATGTCC TGGATAATCT GGTCAACGTA 
ATGAATGGAT ACTGCCTTCC AGATCCTTTT TTTAGCAGCA GGGTAAAAGA CTGGGTAGAG 
CGGTTGATGA AGACCCTCAG AGACCCCTCG TTACCTCTCC TAGAATTACA AGATATCATG 
ACCAGTGTCT CTGGCCGTAT TCCCCCCAAT GTTGAGAAGT CTATCAAGAA GGAAATGGCC 
CAGTATGCCA GCAATATCAC ATCAGTCCTC TGTCAGTTTC CCAGCCAGCA GATTGCCAAC 
ATCCTAGATA GCCATGCAGC TACGTTGAAC CGGAAATCTG AGCGGGAAGT CTTTTTCATG 
AACACTCAGA GCATTGTCCA GCTGGTACAG AGATACCGAA GTGGCATCCG AGGCCACATG 
AAGGCTGTGG TGATGGATCT GCTGCGGCAG TACCTGCGAG TAGAGACACA GTTCCAGAAT 
GGTCACTATG ACAAATGTGT GTTTGCCCTT CGAGAAGAGA ACAAGAGTGA TATGAACACT 
GTACTGAACT ATATCTTCTC TCATGCTCAA GTCACCAAGA AGAATCTTCT GGTCACAATG 
CTTATTGATC AGTTGTGCGG TAGGGACCCT ACTCTCACTG ATGAGCTGCT GAATATTCTC 
ACAGAGCTAA CTCAACTCAG TAAGACCACC AATGCCAAAG TAGCACTTCG AGCACGCCAG 
GTTCTTATTG CCTCCCATTT GCCATCATAT GAGCTTCGCC ATAACCAAGT AGAGTCTATC 
TTCCTTTCAG CTATTGACAT GTATGGACAT CAGTTTTGCA TTGAGAACCT GCAGAAACTC 
ATCTTGTCTG AAACATCTAT TTTTGATGTC CTACCAAACT TCTTCTATCA CAGCAACCAG 
GTAGTGAGGA TGGCAGCTCT GGAGGTTTAT GTTCGAAGGG CTTATATTGC CTATGAACTT 
AACAGCGTAC AACATCGCCA ACTTAAGGAC AACACCTGTG TGGTGGAATT CCAATTCATG 
CTACCCACAT CTCATCCAAA TAGAGGGAAC ATCCCCACGC TAAACAGAAT GTCCTTCTCC 
TCCAACCTCA ACCACTATGG CATGACTCAT GTAGCTAGTG TCAGCGATGT GCTTTTGGAC 
AACTCATTCA CTCCACCATG TCAACGGATG GGCGGAATGG TCTCTTTTCG GACTTTCGAA 
GATTTTGTCA GGATCTTTGA TGAAGTGATG GGCTGCTTCT GTGATTCTCC ACCGCAAAGC 
CCTACATTCC CTGAGGCAGG TCACACATCT CTATACGATG AAGACAAGGT CCCCAGGGAT 
GAACCAATTC ACATTCTGAA TGTGGCTATC AAGACTGACT GTGATATTGA GGATGACAGA 
CTGGCAGCTA TGTTCAGAGA GTTTACCCAA CAAAATAAAG CTACCCTGGT TGAGCATGGG 
ATACGGCGCC TTACTTTCCT GGTTGCACAA AAGGATTTCA GGAAACAGGT CAACTATGAG 
GTGGATCAGA GATTTCATAG AGAATTTCCT AAATTTTTCA CCTTCCGAGC AAGGGATAAG 
TTTGAGGAGG ATCGTATCTA TCGTCACCTG GAACCTGCCC TCGCTTTCCA GTTAGAGCTG 
AACCGGATGA GAAATTTTGA CCTTACTGCC ATTCCATGTG CTAATCATAA AATGCATCTG 
TATCTTGGGG CAGCCAAGGT GGAGGTGGGC ACAGAAGTGA CAGACTACAG GTTCTTTGTT 
CGTGCAATCA TCAGACATTC TGATCTGGTC ACCAAGGAAG CTTCTTTTGA ATACCTACAA 
AATGAAGGGG AGAGGCTGCT TCTAGAAGCC ATGGATGAGT TGGAAGTTGC TTTTAACAAT 
ACAAACGTTC GCACTGACTG TAACCACATC TTCCTCAACT TTGTGCCCAC GGTCATCATG 
GACCCATCAA AGATTGAGGA ATCTGTGCGG AGCATGGTAA TGCGCTATGG AAGTCGGCTG 
TGGAAATTAC GTGTCCTCCA GGCAGAACTG AAAATCAACA TTCGCCTGAC ACCAACTGGA 
AAAGCAATTC CCATCCGCCT CTTCCTGACA AACGAGTCTG GCTATTACTT GGACATCAGC 
TTGTATAAGG AAGTGACTGA CTCCAGGACA GCACAGATCA TGTTTCAGGC ATATGGAGAC 
AAACAGGGAC CATTGCATGG AATGTTAATC AACACTCCAT ACGTCACCAA AGACCTGCTT 
CAATCAAAGA GATTCCAGGC ACAATCCTTG GGGACAACAT ACATATATGA TATACCAGAG 
ATGTTTCGGC AGTCTCTGAT CAAACTCTGG GAGTCTATGT CCACTCAAGC ATTTCTTCCA 
TCACCCCCCC TGCCTTCCGA CATGCTGACA TATACGGAGC TCGTGTTGGA TGATCAAGGT 
CAGCTGGTCC ACATGAACAG GCTTCCAGGA GGAAATGAGA TTGGCATGGT AGCGTGGAAA 
ATGACCCTTA AAAGTCCTGA ATACCCAGAA GGCCGAGATA TCATTGTTAT TGGCAATGAC 
ATCACATACC GAATTGGGTC CTTTGGGCCC CAGGAGGATT TGCTGTTTCT CAGAGCTTCT 
GAGCTTGCCA GGGCAGAGGG TATCCCACGC ATCTTTGTAG CAGCCAACAG TGGAGCAAGA 
ATTGGACTGG CAGAGGAAAT TCGTCATATG TTTCATGTAG CCTGGGTAGA TCCTGAGGAT 
CCTTACAAGG GATACAAATA TTTATATCTG ACCCCTCAGG ATTATAAGAG AGTCAGTGCT 
CTCAACTCTG TCCATTGTGA ACATGTAGAG GACGAAGGAG AATCCAGGTA CAAGATAACT 
GATATTATTG GGAAGGAAGA GGGACTTGGA GCAGAGAACC TTCGAGGCTC TGGAATGATC 
GCTGGAGAAT CCTCATTGGC CTATGATGAG ATCATCACCA TCAGCCTGGT TACATGCCGG 
GCCATTGGGA TTGGAGCTTA CCTTGTCCGG CTGGGACAGA GAACCATCCA GGTTGAAAAT 
TCTCATTTAA TTTTGACTGG AGCTGGAGCC CTCAACAAAG TCCTTGGGCG GGAAGTATAC 
ACCTCCAATA ACCAGCTCGG GGGCATCCAG ATCATGCACA ACAATGGGGT GACCCACAGC 
ATTGTTTGTG ATGACTTTGA AGGGGTTTTC ACTGTCCTGC ACTGGCTATC CTACATGCCT 
AAGAATGTGC GCAGTTTAGT TCCTCTCCTG AACTCCAAGG ATCCTATAGA TAGAATCATC 
GAGTTTGTTC CCACGAAGGC CCCATACGAT CCTCGATGGA TGCTAGCAGG CCGCCCTCAC 
CCAACTCAGA AAGGTCAGTG GCTGAGTGGA TTTTTTGACT ATGGCTCTTT CTCAGAGATC 
ATGCAACCCT GGGCACAAAC TGTGGTGGTT GGCAGAGCCA GGCTAGGAGG AATACCTGTG 
GGAGTAGTTG CTGTAGAAAC CCGAACAGTG GAACTAAGTA TCCCAGCCGA TCCTGCAAAC 
CTGGATTCTG AAGCCAAGAT AATCCAGCAG GCTGGCCAGG TTTGGTTCCC AGACTCTGCG 
TTTAAGACCT ATCAGGCTAT CAAGGACTTC AACCGTGAAG GACTGCCTCT GATGGTCTTT 
GCCAACTGGA GAGGCTTCTC TGGTGGGATG AAAGATATGT ATGACCAAGT GCTGAAGTTT 
GGTGCCTATA TTGTGGATAG CTTACGGGAG TGCTCCCAGC CTGTGCTGGT ATACATTCCT 
CCTCAGGCTG AGCTCCGGGG TGGCTCCTGG GTTGTGATTG ACCCCACCAT CAACCCCCGG 
CATATGGAGA TGTACGCTGA CCGAGAAAGC AGGGGATCAG TTCTGGAGCC AGAAGGGACA 
GTAGAAATCA AATTCCGCAG AAAGGATCTG GTGAAAACCA TGCGTCGGGT GGACCCAGTC 
TACATTCGCT TGGCTGAGCG ATTGGGCACC CCAGAGCTCA GCGCAGCTGA GCGGAAGGAG 
CTGGAGAACA AGTTGAAGGA GCGGGAGGAA TTCCTAATTC CCATTTACCA TCAGGTAGCC 
GTGCAGTTTG CTGACTTGCA CGACACACCA GGCCGGATGC AGGAGAAGGG TGTTATTAAT 
GACATCCTGG ATTGGAAAAC ATCCCGTACC TTTTTCTATT GGCGGCTGAG GCGTCTCCTG 
CTGGAGGACC TGGTCAAAAA GAAAATCCAC AATGCCAACC CAGAGCTGAC CGATGGCCAG 
ATCCAGGCCA TGCTACGGCG CTGGTTTGTA GAAGTGGAAG GAACTGTGAA GGCCTACGTT 
TGGGACAATA ACAAGGACCT GGTGGAGTGG CTGGAGAAAC AGCTGACCGA GGAAGATGGT 
GTTCGCTCAG TAATCGAGGA AAACATCAAA TATATCAGCA GAGACTATGT CCTCAAGCAG 
ATCCGCAGCT TGGTACAGGC CAATCCCGAG GTTGCCATGG ACTCCATCGT CCATATGACT 
CAGCATATCT CACCCACTCA GCGAGCAGAA GTCGTACGGA TCCTCTCCAC GATGGACTCC 
CCTTCAACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624832.1
CDS	314..7465
Translation	MWWSTLMSILRVSSFWRWISAQTVRIIRALRAHFERIMDEPSPLAKPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_005624775.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624775.3

ORF Insert Sequence:

ATGTGGTGGT CCACTCTGAT GTCAATCTTG AGGGTTAGTT CTTTTTGGAG GTGGATATCT 
GCTCAGACAG TGAGAATTAT AAGAGCTTTG AGAGCTCATT TTGAAAGAAT AATGGATGAA 
CCATCCCCCT TGGCCAAACC TCTGGAGCTG AACCAGCACT CCCGATTCAT AATTGGTTCT 
GTGTCTGAAG ATAACTCAGA GGATGAAATT AGCAACCTGG TGAAGCTGGA TCTCCTGGAG 
GAGAAGGAGG GCTCTCTATC ACCAGCTTCT GTTGGCTCAG ATACACTTTC TGACTTGGGG 
ATCTCCAGCC TACAGGACGG CTTGGCCTTA CACATGAGGT CCAGCATGTC TGGCTTGCAT 
CTAGTGAAGC AAGGTCGAGA CCGAAAGAAA ATAGACTCTC AGCGAGATTT CACTGTAGCT 
TCTCCAGCAG AATTTGTTAC TCGTTTTGGG GGAAATAAAG TGATTGAGAA GGTTCTTATT 
GCCAACAATG GCATTGCAGC AGTGAAATGC ATGCGGTCTA TCCGTCGATG GTCTTATGAG 
ATGTTTCGAA ATGAACGTGC AATCCGATTT GTTGTTATGG TAACACCTGA AGACCTGAAA 
GCCAATGCAG AATACATTAA GATGGCGGAT CACTATGTGC CAGTGCCAGG AGGACCAAAT 
AACAACAACT ATGCGAACGT GGAATTAATT CTTGATATTG CTAAAAGGAT TCCCGTACAA 
GCAGTGTGGG CTGGCTGGGG TCATGCTTCT GAGAATCCCA AGCTCCCAGA ACTTCTTTTG 
AAAAATGGCA TCGCCTTCAT GGGCCCTCCA AGCCAGGCCA TGTGGGCTTT GGGGGATAAG 
ATTGCATCTT CTATAGTGGC TCAAACTGCA GGTATCCCAA CTCTTCCCTG GAGTGGCAGT 
GGTCTTCGGG TGGACTGGCA TGAAAATGAT TTTTCCAAAC GTATCTTAAA TGTTCCCCAG 
GAATTATACG AAAAAGGTTA TGTGAAGGAT GTGGATGATG GGCTAAAGGC AGCGGAGGAA 
GTTGGATATC CAGTAATGAT CAAGGCCTCA GAAGGAGGAG GAGGGAAAGG AATCAGAAAA 
GTCAACAATG CAGATGATTT CCCTAACCTC TTCAGACAGG TTCAAGCTGA AGTCCCTGGA 
TCTCCTATAT TTGTGATGAG ACTAGCCAAA CAATCTCGTC ATCTGGAGGT ACAGATCTTA 
GCAGATCAAT ATGGCAATGC TATCTCTCTC TTTGGTCGTG ACTGCTCTGT ACAGCGCAGG 
CATCAGAAGA TTATTGAGGA AGCACCTGCT GCTATTGCTA CTCCAGCAGT ATTTGAACAT 
ATGGAACAGT GTGCTGTGAA ACTTGCCAAA ATGGTTGGTT ATGTGAGTGC TGGGACTGTG 
GAATACCTCT ACAGCCAGGA TGGAAGCTTC TACTTTCTGG AACTGAACCC TCGGCTACAG 
GTGGAACACC CTTGTACAGA GATGGTGGCT GATGTCAACC TCCCTGCAGC ACAACTCCAG 
ATTGCCATGG GGATCCCTCT GTACAGAATC AAGGATATTC GTATGATGTA TGGGGTATCT 
CCCTGGGGTG ATGCTCCCAT TGATTTTGAA AATTCTGCTC ACGTTCCTTG CCCAAGGGGC 
CATGTTATTG CCGCTCGGAT CACTAGTGAA AATCCAGATG AGGGTTTTAA GCCCAGCTCA 
GGAACAGTTC AGGAACTGAA TTTCCGTAGC AATAAGAATG TTTGGGGTTA TTTCAGTGTT 
GCTGCTGCAG GGGGCCTTCA TGAATTTGCT GATTCTCAGT TTGGTCATTG CTTCTCCTGG 
GGAGAAAACA GAGAGGAAGC AATTTCAAAC ATGGTGGTGG CTTTGAAGGA GCTGTCTATT 
CGGGGTGACT TTCGAACCAC AGTTGAATAC CTCATCAAAT TGTTGGAGAC TGAAAGCTTT 
CAGTTGAATA GAATTGACAC CGGCTGGCTG GACAGACTGA TAGCAGAGAA AGTACAGGCA 
GAGCGACCTG ACACCATGTT GGGAGTTGTT TGTGGGGCTC TCCATGTGGC AGATGTGAGC 
CTACGGAATA GCATCTCTAA CTTCCTTCAT TCTTTAGAAA GGGGTCAAGT CCTTCCTGCT 
CATACACTTC TGAATACAGT AGATGTTGAA CTTATCTATG GGGGAGTCAA ATATGTACTT 
AAGGTGACTC GGCAGTCCCC CAACTCCTAT GTGGTGATCA TGAATGGTTC ATGCGTAGAA 
GTAGATGTGC ATCGGCTCAG TGATGGAGGG TTACTCCTGT CCTATGATGG CAGCAGTTAC 
ACCACATACA TGAAGGAAGA AGTGGATAGA TATCGTATCA CAATTGGCAA TAAGACCTGT 
GTGTTTGAGA AGGAAAATGA CCCTTCAGTG TTACGCTCAC CTTCTGCTGG GAAGTTAATC 
CAGTACATTG TGGAGGATGG AGGCCATGTG TTTGCTGGCC AGTGCTATGC TGAAATTGAG 
GTGATGAAGA TGGTAATGAC CTTAACAGCT GCAGAGTCTG GCTGTATCCA TTATGTCAAA 
CGGCCTGGAG CAGCTCTTGA CCCTGGCTGT GTAATAGCCA AAATGCAGTT GGACAACCCC 
AGCAAGGTCC AACAGGCTGA GCTTCACACA GGTAGTCTGC CACGGATCCA GAGCACTGCA 
CTCAGAGGCG AGAAGCTCCA TCGAGTGTTC CACTATGTCC TGGATAATCT GGTCAACGTA 
ATGAATGGAT ACTGCCTTCC AGATCCTTTT TTTAGCAGCA GGGTAAAAGA CTGGGTAGAG 
CGGTTGATGA AGACCCTCAG AGACCCCTCG TTACCTCTCC TAGAATTACA AGATATCATG 
ACCAGTGTCT CTGGCCGTAT TCCCCCCAAT GTTGAGAAGT CTATCAAGAA GGAAATGGCC 
CAGTATGCCA GCAATATCAC ATCAGTCCTC TGTCAGTTTC CCAGCCAGCA GATTGCCAAC 
ATCCTAGATA GCCATGCAGC TACGTTGAAC CGGAAATCTG AGCGGGAAGT CTTTTTCATG 
AACACTCAGA GCATTGTCCA GCTGGTACAG AGATACCGAA GTGGCATCCG AGGCCACATG 
AAGGCTGTGG TGATGGATCT GCTGCGGCAG TACCTGCGAG TAGAGACACA GTTCCAGAAT 
GGTCACTATG ACAAATGTGT GTTTGCCCTT CGAGAAGAGA ACAAGAGTGA TATGAACACT 
GTACTGAACT ATATCTTCTC TCATGCTCAA GTCACCAAGA AGAATCTTCT GGTCACAATG 
CTTATTGATC AGTTGTGCGG TAGGGACCCT ACTCTCACTG ATGAGCTGCT GAATATTCTC 
ACAGAGCTAA CTCAACTCAG TAAGACCACC AATGCCAAAG TAGCACTTCG AGCACGCCAG 
GTTCTTATTG CCTCCCATTT GCCATCATAT GAGCTTCGCC ATAACCAAGT AGAGTCTATC 
TTCCTTTCAG CTATTGACAT GTATGGACAT CAGTTTTGCA TTGAGAACCT GCAGAAACTC 
ATCTTGTCTG AAACATCTAT TTTTGATGTC CTACCAAACT TCTTCTATCA CAGCAACCAG 
GTAGTGAGGA TGGCAGCTCT GGAGGTTTAT GTTCGAAGGG CTTATATTGC CTATGAACTT 
AACAGCGTAC AACATCGCCA ACTTAAGGAC AACACCTGTG TGGTGGAATT CCAATTCATG 
CTACCCACAT CTCATCCAAA TAGAGGGAAC ATCCCCACGC TAAACAGAAT GTCCTTCTCC 
TCCAACCTCA ACCACTATGG CATGACTCAT GTAGCTAGTG TCAGCGATGT GCTTTTGGAC 
AACTCATTCA CTCCACCATG TCAACGGATG GGCGGAATGG TCTCTTTTCG GACTTTCGAA 
GATTTTGTCA GGATCTTTGA TGAAGTGATG GGCTGCTTCT GTGATTCTCC ACCGCAAAGC 
CCTACATTCC CTGAGGCAGG TCACACATCT CTATACGATG AAGACAAGGT CCCCAGGGAT 
GAACCAATTC ACATTCTGAA TGTGGCTATC AAGACTGACT GTGATATTGA GGATGACAGA 
CTGGCAGCTA TGTTCAGAGA GTTTACCCAA CAAAATAAAG CTACCCTGGT TGAGCATGGG 
ATACGGCGCC TTACTTTCCT GGTTGCACAA AAGGATTTCA GGAAACAGGT CAACTATGAG 
GTGGATCAGA GATTTCATAG AGAATTTCCT AAATTTTTCA CCTTCCGAGC AAGGGATAAG 
TTTGAGGAGG ATCGTATCTA TCGTCACCTG GAACCTGCCC TCGCTTTCCA GTTAGAGCTG 
AACCGGATGA GAAATTTTGA CCTTACTGCC ATTCCATGTG CTAATCATAA AATGCATCTG 
TATCTTGGGG CAGCCAAGGT GGAGGTGGGC ACAGAAGTGA CAGACTACAG GTTCTTTGTT 
CGTGCAATCA TCAGACATTC TGATCTGGTC ACCAAGGAAG CTTCTTTTGA ATACCTACAA 
AATGAAGGGG AGAGGCTGCT TCTAGAAGCC ATGGATGAGT TGGAAGTTGC TTTTAACAAT 
ACAAACGTTC GCACTGACTG TAACCACATC TTCCTCAACT TTGTGCCCAC GGTCATCATG 
GACCCATCAA AGATTGAGGA ATCTGTGCGG AGCATGGTAA TGCGCTATGG AAGTCGGCTG 
TGGAAATTAC GTGTCCTCCA GGCAGAACTG AAAATCAACA TTCGCCTGAC ACCAACTGGA 
AAAGCAATTC CCATCCGCCT CTTCCTGACA AACGAGTCTG GCTATTACTT GGACATCAGC 
TTGTATAAGG AAGTGACTGA CTCCAGGACA GCACAGATCA TGTTTCAGGC ATATGGAGAC 
AAACAGGGAC CATTGCATGG AATGTTAATC AACACTCCAT ACGTCACCAA AGACCTGCTT 
CAATCAAAGA GATTCCAGGC ACAATCCTTG GGGACAACAT ACATATATGA TATACCAGAG 
ATGTTTCGGC AGTCTCTGAT CAAACTCTGG GAGTCTATGT CCACTCAAGC ATTTCTTCCA 
TCACCCCCCC TGCCTTCCGA CATGCTGACA TATACGGAGC TCGTGTTGGA TGATCAAGGT 
CAGCTGGTCC ACATGAACAG GCTTCCAGGA GGAAATGAGA TTGGCATGGT AGCGTGGAAA 
ATGACCCTTA AAAGTCCTGA ATACCCAGAA GGCCGAGATA TCATTGTTAT TGGCAATGAC 
ATCACATACC GAATTGGGTC CTTTGGGCCC CAGGAGGATT TGCTGTTTCT CAGAGCTTCT 
GAGCTTGCCA GGGCAGAGGG TATCCCACGC ATCTTTGTAG CAGCCAACAG TGGAGCAAGA 
ATTGGACTGG CAGAGGAAAT TCGTCATATG TTTCATGTAG CCTGGGTAGA TCCTGAGGAT 
CCTTACAAGG GATACAAATA TTTATATCTG ACCCCTCAGG ATTATAAGAG AGTCAGTGCT 
CTCAACTCTG TCCATTGTGA ACATGTAGAG GACGAAGGAG AATCCAGGTA CAAGATAACT 
GATATTATTG GGAAGGAAGA GGGACTTGGA GCAGAGAACC TTCGAGGCTC TGGAATGATC 
GCTGGAGAAT CCTCATTGGC CTATGATGAG ATCATCACCA TCAGCCTGGT TACATGCCGG 
GCCATTGGGA TTGGAGCTTA CCTTGTCCGG CTGGGACAGA GAACCATCCA GGTTGAAAAT 
TCTCATTTAA TTTTGACTGG AGCTGGAGCC CTCAACAAAG TCCTTGGGCG GGAAGTATAC 
ACCTCCAATA ACCAGCTCGG GGGCATCCAG ATCATGCACA ACAATGGGGT GACCCACAGC 
ATTGTTTGTG ATGACTTTGA AGGGGTTTTC ACTGTCCTGC ACTGGCTATC CTACATGCCT 
AAGAATGTGC GCAGTTTAGT TCCTCTCCTG AACTCCAAGG ATCCTATAGA TAGAATCATC 
GAGTTTGTTC CCACGAAGGC CCCATACGAT CCTCGATGGA TGCTAGCAGG CCGCCCTCAC 
CCAACTCAGA AAGGTCAGTG GCTGAGTGGA TTTTTTGACT ATGGCTCTTT CTCAGAGATC 
ATGCAACCCT GGGCACAAAC TGTGGTGGTT GGCAGAGCCA GGCTAGGAGG AATACCTGTG 
GGAGTAGTTG CTGTAGAAAC CCGAACAGTG GAACTAAGTA TCCCAGCCGA TCCTGCAAAC 
CTGGATTCTG AAGCCAAGAT AATCCAGCAG GCTGGCCAGG TTTGGTTCCC AGACTCTGCG 
TTTAAGACCT ATCAGGCTAT CAAGGACTTC AACCGTGAAG GACTGCCTCT GATGGTCTTT 
GCCAACTGGA GAGGCTTCTC TGGTGGGATG AAAGATATGT ATGACCAAGT GCTGAAGTTT 
GGTGCCTATA TTGTGGATAG CTTACGGGAG TGCTCCCAGC CTGTGCTGGT ATACATTCCT 
CCTCAGGCTG AGCTCCGGGG TGGCTCCTGG GTTGTGATTG ACCCCACCAT CAACCCCCGG 
CATATGGAGA TGTACGCTGA CCGAGAAAGC AGGGGATCAG TTCTGGAGCC AGAAGGGACA 
GTAGAAATCA AATTCCGCAG AAAGGATCTG GTGAAAACCA TGCGTCGGGT GGACCCAGTC 
TACATTCGCT TGGCTGAGCG ATTGGGCACC CCAGAGCTCA GCGCAGCTGA GCGGAAGGAG 
CTGGAGAACA AGTTGAAGGA GCGGGAGGAA TTCCTAATTC CCATTTACCA TCAGGTAGCC 
GTGCAGTTTG CTGACTTGCA CGACACACCA GGCCGGATGC AGGAGAAGGG TGTTATTAAT 
GACATCCTGG ATTGGAAAAC ATCCCGTACC TTTTTCTATT GGCGGCTGAG GCGTCTCCTG 
CTGGAGGACC TGGTCAAAAA GAAAATCCAC AATGCCAACC CAGAGCTGAC CGATGGCCAG 
ATCCAGGCCA TGCTACGGCG CTGGTTTGTA GAAGTGGAAG GAACTGTGAA GGCCTACGTT 
TGGGACAATA ACAAGGACCT GGTGGAGTGG CTGGAGAAAC AGCTGACCGA GGAAGATGGT 
GTTCGCTCAG TAATCGAGGA AAACATCAAA TATATCAGCA GAGACTATGT CCTCAAGCAG 
ATCCGCAGCT TGGTACAGGC CAATCCCGAG GTTGCCATGG ACTCCATCGT CCATATGACT 
CAGCATATCT CACCCACTCA GCGAGCAGAA GTCGTACGGA TCCTCTCCAC GATGGACTCC 
CCTTCAACGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_005624776.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624833.1 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X2, mRNA.

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CloneID	OCe12091
Clone ID Related Accession (Same CDS sequence)	XM_005624776.2 , XM_005624776.3
Accession Version	XM_005624776.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	acetyl-CoA carboxylase 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624776.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGGT CCACTCTGAT GTCAATCTTG AGGGTTAGTT CTTTTTGGAG GTGGATATCT 
GCTCAGACAG TGAGAATTAT AAGAGCTTTG AGAGCTCATT TTGAAAGAAT AATGGATGAA 
CCATCCCCCT TGGCCAAACC TCTGGAGCTG AACCAGCACT CCCGATTCAT AATTGGTTCT 
GTGTCTGAAG ATAACTCAGA GGATGAAATT AGCAACCTGG TGAAGCTGGA TCTCCTGGAG 
GAGAAGGAGG GCTCTCTATC ACCAGCTTCT GTTGGCTCAG ATACACTTTC TGACTTGGGG 
ATCTCCAGCC TACAGGACGG CTTGGCCTTA CACATGAGGT CCAGCATGTC TGGCTTGCAT 
CTAGTGAAGC AAGGTCGAGA CCGAAAGAAA ATAGACTCTC AGCGAGATTT CACTGTAGCT 
TCTCCAGCAG AATTTGTTAC TCGTTTTGGG GGAAATAAAG TGATTGAGAA GGTTCTTATT 
GCCAACAATG GCATTGCAGC AGTGAAATGC ATGCGGTCTA TCCGTCGATG GTCTTATGAG 
ATGTTTCGAA ATGAACGTGC AATCCGATTT GTTGTTATGG TAACACCTGA AGACCTGAAA 
GCCAATGCAG AATACATTAA GATGGCGGAT CACTATGTGC CAGTGCCAGG AGGACCAAAT 
AACAACAACT ATGCGAACGT GGAATTAATT CTTGATATTG CTAAAAGGAT TCCCGTACAA 
GCAGTGTGGG CTGGCTGGGG TCATGCTTCT GAGAATCCCA AGCTCCCAGA ACTTCTTTTG 
AAAAATGGCA TCGCCTTCAT GGGCCCTCCA AGCCAGGCCA TGTGGGCTTT GGGGGATAAG 
ATTGCATCTT CTATAGTGGC TCAAACTGCA GGTATCCCAA CTCTTCCCTG GAGTGGCAGT 
GGTCTTCGGG TGGACTGGCA TGAAAATGAT TTTTCCAAAC GTATCTTAAA TGTTCCCCAG 
GAATTATACG AAAAAGGTTA TGTGAAGGAT GTGGATGATG GGCTAAAGGC AGCGGAGGAA 
GTTGGATATC CAGTAATGAT CAAGGCCTCA GAAGGAGGAG GAGGGAAAGG AATCAGAAAA 
GTCAACAATG CAGATGATTT CCCTAACCTC TTCAGACAGG TTCAAGCTGA AGTCCCTGGA 
TCTCCTATAT TTGTGATGAG ACTAGCCAAA CAATCTCGTC ATCTGGAGGT ACAGATCTTA 
GCAGATCAAT ATGGCAATGC TATCTCTCTC TTTGGTCGTG ACTGCTCTGT ACAGCGCAGG 
CATCAGAAGA TTATTGAGGA AGCACCTGCT GCTATTGCTA CTCCAGCAGT ATTTGAACAT 
ATGGAACAGT GTGCTGTGAA ACTTGCCAAA ATGGTTGGTT ATGTGAGTGC TGGGACTGTG 
GAATACCTCT ACAGCCAGGA TGGAAGCTTC TACTTTCTGG AACTGAACCC TCGGCTACAG 
GTGGAACACC CTTGTACAGA GATGGTGGCT GATGTCAACC TCCCTGCAGC ACAACTCCAG 
ATTGCCATGG GGATCCCTCT GTACAGAATC AAGGATATTC GTATGATGTA TGGGGTATCT 
CCCTGGGGTG ATGCTCCCAT TGATTTTGAA AATTCTGCTC ACGTTCCTTG CCCAAGGGGC 
CATGTTATTG CCGCTCGGAT CACTAGTGAA AATCCAGATG AGGGTTTTAA GCCCAGCTCA 
GGAACAGTTC AGGAACTGAA TTTCCGTAGC AATAAGAATG TTTGGGGTTA TTTCAGTGTT 
GCTGCTGCAG GGGGCCTTCA TGAATTTGCT GATTCTCAGT TTGGTCATTG CTTCTCCTGG 
GGAGAAAACA GAGAGGAAGC AATTTCAAAC ATGGTGGTGG CTTTGAAGGA GCTGTCTATT 
CGGGGTGACT TTCGAACCAC AGTTGAATAC CTCATCAAAT TGTTGGAGAC TGAAAGCTTT 
CAGTTGAATA GAATTGACAC CGGCTGGCTG GACAGACTGA TAGCAGAGAA AGTACAGGCA 
GAGCGACCTG ACACCATGTT GGGAGTTGTT TGTGGGGCTC TCCATGTGGC AGATGTGAGC 
CTACGGAATA GCATCTCTAA CTTCCTTCAT TCTTTAGAAA GGGGTCAAGT CCTTCCTGCT 
CATACACTTC TGAATACAGT AGATGTTGAA CTTATCTATG GGGGAGTCAA ATATGTACTT 
AAGGTGACTC GGCAGTCCCC CAACTCCTAT GTGGTGATCA TGAATGGTTC ATGCGTAGAA 
GTAGATGTGC ATCGGCTCAG TGATGGAGGG TTACTCCTGT CCTATGATGG CAGCAGTTAC 
ACCACATACA TGAAGGAAGA AGTGGATAGA TATCGTATCA CAATTGGCAA TAAGACCTGT 
GTGTTTGAGA AGGAAAATGA CCCTTCAGTG TTACGCTCAC CTTCTGCTGG GAAGTTAATC 
CAGTACATTG TGGAGGATGG AGGCCATGTG TTTGCTGGCC AGTGCTATGC TGAAATTGAG 
GTGATGAAGA TGGTAATGAC CTTAACAGCT GCAGAGTCTG GCTGTATCCA TTATGTCAAA 
CGGCCTGGAG CAGCTCTTGA CCCTGGCTGT GTAATAGCCA AAATGCAGTT GGACAACCCC 
AGCAAGGTCC AACAGGCTGA GCTTCACACA GGTAGTCTGC CACGGATCCA GAGCACTGCA 
CTCAGAGGCG AGAAGCTCCA TCGAGTGTTC CACTATGTCC TGGATAATCT GGTCAACGTA 
ATGAATGGAT ACTGCCTTCC AGATCCTTTT TTTAGCAGCA GGGTAAAAGA CTGGGTAGAG 
CGGTTGATGA AGACCCTCAG AGACCCCTCG TTACCTCTCC TAGAATTACA AGATATCATG 
ACCAGTGTCT CTGGCCGTAT TCCCCCCAAT GTTGAGAAGT CTATCAAGAA GGAAATGGCC 
CAGTATGCCA GCAATATCAC ATCAGTCCTC TGTCAGTTTC CCAGCCAGCA GATTGCCAAC 
ATCCTAGATA GCCATGCAGC TACGTTGAAC CGGAAATCTG AGCGGGAAGT CTTTTTCATG 
AACACTCAGA GCATTGTCCA GCTGGTACAG AGATACCGAA GTGGCATCCG AGGCCACATG 
AAGGCTGTGG TGATGGATCT GCTGCGGCAG TACCTGCGAG TAGAGACACA GTTCCAGAAT 
GGTCACTATG ACAAATGTGT GTTTGCCCTT CGAGAAGAGA ACAAGAGTGA TATGAACACT 
GTACTGAACT ATATCTTCTC TCATGCTCAA GTCACCAAGA AGAATCTTCT GGTCACAATG 
CTTATTGATC AGTTGTGCGG TAGGGACCCT ACTCTCACTG ATGAGCTGCT GAATATTCTC 
ACAGAGCTAA CTCAACTCAG TAAGACCACC AATGCCAAAG TAGCACTTCG AGCACGCCAG 
GTTCTTATTG CCTCCCATTT GCCATCATAT GAGCTTCGCC ATAACCAAGT AGAGTCTATC 
TTCCTTTCAG CTATTGACAT GTATGGACAT CAGTTTTGCA TTGAGAACCT GCAGAAACTC 
ATCTTGTCTG AAACATCTAT TTTTGATGTC CTACCAAACT TCTTCTATCA CAGCAACCAG 
GTAGTGAGGA TGGCAGCTCT GGAGGTTTAT GTTCGAAGGG CTTATATTGC CTATGAACTT 
AACAGCGTAC AACATCGCCA ACTTAAGGAC AACACCTGTG TGGTGGAATT CCAATTCATG 
CTACCCACAT CTCATCCAAA TAGAATGTCC TTCTCCTCCA ACCTCAACCA CTATGGCATG 
ACTCATGTAG CTAGTGTCAG CGATGTGCTT TTGGACAACT CATTCACTCC ACCATGTCAA 
CGGATGGGCG GAATGGTCTC TTTTCGGACT TTCGAAGATT TTGTCAGGAT CTTTGATGAA 
GTGATGGGCT GCTTCTGTGA TTCTCCACCG CAAAGCCCTA CATTCCCTGA GGCAGGTCAC 
ACATCTCTAT ACGATGAAGA CAAGGTCCCC AGGGATGAAC CAATTCACAT TCTGAATGTG 
GCTATCAAGA CTGACTGTGA TATTGAGGAT GACAGACTGG CAGCTATGTT CAGAGAGTTT 
ACCCAACAAA ATAAAGCTAC CCTGGTTGAG CATGGGATAC GGCGCCTTAC TTTCCTGGTT 
GCACAAAAGG ATTTCAGGAA ACAGGTCAAC TATGAGGTGG ATCAGAGATT TCATAGAGAA 
TTTCCTAAAT TTTTCACCTT CCGAGCAAGG GATAAGTTTG AGGAGGATCG TATCTATCGT 
CACCTGGAAC CTGCCCTCGC TTTCCAGTTA GAGCTGAACC GGATGAGAAA TTTTGACCTT 
ACTGCCATTC CATGTGCTAA TCATAAAATG CATCTGTATC TTGGGGCAGC CAAGGTGGAG 
GTGGGCACAG AAGTGACAGA CTACAGGTTC TTTGTTCGTG CAATCATCAG ACATTCTGAT 
CTGGTCACCA AGGAAGCTTC TTTTGAATAC CTACAAAATG AAGGGGAGAG GCTGCTTCTA 
GAAGCCATGG ATGAGTTGGA AGTTGCTTTT AACAATACAA ACGTTCGCAC TGACTGTAAC 
CACATCTTCC TCAACTTTGT GCCCACGGTC ATCATGGACC CATCAAAGAT TGAGGAATCT 
GTGCGGAGCA TGGTAATGCG CTATGGAAGT CGGCTGTGGA AATTACGTGT CCTCCAGGCA 
GAACTGAAAA TCAACATTCG CCTGACACCA ACTGGAAAAG CAATTCCCAT CCGCCTCTTC 
CTGACAAACG AGTCTGGCTA TTACTTGGAC ATCAGCTTGT ATAAGGAAGT GACTGACTCC 
AGGACAGCAC AGATCATGTT TCAGGCATAT GGAGACAAAC AGGGACCATT GCATGGAATG 
TTAATCAACA CTCCATACGT CACCAAAGAC CTGCTTCAAT CAAAGAGATT CCAGGCACAA 
TCCTTGGGGA CAACATACAT ATATGATATA CCAGAGATGT TTCGGCAGTC TCTGATCAAA 
CTCTGGGAGT CTATGTCCAC TCAAGCATTT CTTCCATCAC CCCCCCTGCC TTCCGACATG 
CTGACATATA CGGAGCTCGT GTTGGATGAT CAAGGTCAGC TGGTCCACAT GAACAGGCTT 
CCAGGAGGAA ATGAGATTGG CATGGTAGCG TGGAAAATGA CCCTTAAAAG TCCTGAATAC 
CCAGAAGGCC GAGATATCAT TGTTATTGGC AATGACATCA CATACCGAAT TGGGTCCTTT 
GGGCCCCAGG AGGATTTGCT GTTTCTCAGA GCTTCTGAGC TTGCCAGGGC AGAGGGTATC 
CCACGCATCT TTGTAGCAGC CAACAGTGGA GCAAGAATTG GACTGGCAGA GGAAATTCGT 
CATATGTTTC ATGTAGCCTG GGTAGATCCT GAGGATCCTT ACAAGGGATA CAAATATTTA 
TATCTGACCC CTCAGGATTA TAAGAGAGTC AGTGCTCTCA ACTCTGTCCA TTGTGAACAT 
GTAGAGGACG AAGGAGAATC CAGGTACAAG ATAACTGATA TTATTGGGAA GGAAGAGGGA 
CTTGGAGCAG AGAACCTTCG AGGCTCTGGA ATGATCGCTG GAGAATCCTC ATTGGCCTAT 
GATGAGATCA TCACCATCAG CCTGGTTACA TGCCGGGCCA TTGGGATTGG AGCTTACCTT 
GTCCGGCTGG GACAGAGAAC CATCCAGGTT GAAAATTCTC ATTTAATTTT GACTGGAGCT 
GGAGCCCTCA ACAAAGTCCT TGGGCGGGAA GTATACACCT CCAATAACCA GCTCGGGGGC 
ATCCAGATCA TGCACAACAA TGGGGTGACC CACAGCATTG TTTGTGATGA CTTTGAAGGG 
GTTTTCACTG TCCTGCACTG GCTATCCTAC ATGCCTAAGA ATGTGCGCAG TTTAGTTCCT 
CTCCTGAACT CCAAGGATCC TATAGATAGA ATCATCGAGT TTGTTCCCAC GAAGGCCCCA 
TACGATCCTC GATGGATGCT AGCAGGCCGC CCTCACCCAA CTCAGAAAGG TCAGTGGCTG 
AGTGGATTTT TTGACTATGG CTCTTTCTCA GAGATCATGC AACCCTGGGC ACAAACTGTG 
GTGGTTGGCA GAGCCAGGCT AGGAGGAATA CCTGTGGGAG TAGTTGCTGT AGAAACCCGA 
ACAGTGGAAC TAAGTATCCC AGCCGATCCT GCAAACCTGG ATTCTGAAGC CAAGATAATC 
CAGCAGGCTG GCCAGGTTTG GTTCCCAGAC TCTGCGTTTA AGACCTATCA GGCTATCAAG 
GACTTCAACC GTGAAGGACT GCCTCTGATG GTCTTTGCCA ACTGGAGAGG CTTCTCTGGT 
GGGATGAAAG ATATGTATGA CCAAGTGCTG AAGTTTGGTG CCTATATTGT GGATAGCTTA 
CGGGAGTGCT CCCAGCCTGT GCTGGTATAC ATTCCTCCTC AGGCTGAGCT CCGGGGTGGC 
TCCTGGGTTG TGATTGACCC CACCATCAAC CCCCGGCATA TGGAGATGTA CGCTGACCGA 
GAAAGCAGGG GATCAGTTCT GGAGCCAGAA GGGACAGTAG AAATCAAATT CCGCAGAAAG 
GATCTGGTGA AAACCATGCG TCGGGTGGAC CCAGTCTACA TTCGCTTGGC TGAGCGATTG 
GGCACCCCAG AGCTCAGCGC AGCTGAGCGG AAGGAGCTGG AGAACAAGTT GAAGGAGCGG 
GAGGAATTCC TAATTCCCAT TTACCATCAG GTAGCCGTGC AGTTTGCTGA CTTGCACGAC 
ACACCAGGCC GGATGCAGGA GAAGGGTGTT ATTAATGACA TCCTGGATTG GAAAACATCC 
CGTACCTTTT TCTATTGGCG GCTGAGGCGT CTCCTGCTGG AGGACCTGGT CAAAAAGAAA 
ATCCACAATG CCAACCCAGA GCTGACCGAT GGCCAGATCC AGGCCATGCT ACGGCGCTGG 
TTTGTAGAAG TGGAAGGAAC TGTGAAGGCC TACGTTTGGG ACAATAACAA GGACCTGGTG 
GAGTGGCTGG AGAAACAGCT GACCGAGGAA GATGGTGTTC GCTCAGTAAT CGAGGAAAAC 
ATCAAATATA TCAGCAGAGA CTATGTCCTC AAGCAGATCC GCAGCTTGGT ACAGGCCAAT 
CCCGAGGTTG CCATGGACTC CATCGTCCAT ATGACTCAGC ATATCTCACC CACTCAGCGA 
GCAGAAGTCG TACGGATCCT CTCCACGATG GACTCCCCTT CAACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624833.1
CDS	434..7561
Translation	MWWSTLMSILRVSSFWRWISAQTVRIIRALRAHFERIMDEPSPLAKPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_005624776.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624776.2

ORF Insert Sequence:

ATGTGGTGGT CCACTCTGAT GTCAATCTTG AGGGTTAGTT CTTTTTGGAG GTGGATATCT 
GCTCAGACAG TGAGAATTAT AAGAGCTTTG AGAGCTCATT TTGAAAGAAT AATGGATGAA 
CCATCCCCCT TGGCCAAACC TCTGGAGCTG AACCAGCACT CCCGATTCAT AATTGGTTCT 
GTGTCTGAAG ATAACTCAGA GGATGAAATT AGCAACCTGG TGAAGCTGGA TCTCCTGGAG 
GAGAAGGAGG GCTCTCTATC ACCAGCTTCT GTTGGCTCAG ATACACTTTC TGACTTGGGG 
ATCTCCAGCC TACAGGACGG CTTGGCCTTA CACATGAGGT CCAGCATGTC TGGCTTGCAT 
CTAGTGAAGC AAGGTCGAGA CCGAAAGAAA ATAGACTCTC AGCGAGATTT CACTGTAGCT 
TCTCCAGCAG AATTTGTTAC TCGTTTTGGG GGAAATAAAG TGATTGAGAA GGTTCTTATT 
GCCAACAATG GCATTGCAGC AGTGAAATGC ATGCGGTCTA TCCGTCGATG GTCTTATGAG 
ATGTTTCGAA ATGAACGTGC AATCCGATTT GTTGTTATGG TAACACCTGA AGACCTGAAA 
GCCAATGCAG AATACATTAA GATGGCGGAT CACTATGTGC CAGTGCCAGG AGGACCAAAT 
AACAACAACT ATGCGAACGT GGAATTAATT CTTGATATTG CTAAAAGGAT TCCCGTACAA 
GCAGTGTGGG CTGGCTGGGG TCATGCTTCT GAGAATCCCA AGCTCCCAGA ACTTCTTTTG 
AAAAATGGCA TCGCCTTCAT GGGCCCTCCA AGCCAGGCCA TGTGGGCTTT GGGGGATAAG 
ATTGCATCTT CTATAGTGGC TCAAACTGCA GGTATCCCAA CTCTTCCCTG GAGTGGCAGT 
GGTCTTCGGG TGGACTGGCA TGAAAATGAT TTTTCCAAAC GTATCTTAAA TGTTCCCCAG 
GAATTATACG AAAAAGGTTA TGTGAAGGAT GTGGATGATG GGCTAAAGGC AGCGGAGGAA 
GTTGGATATC CAGTAATGAT CAAGGCCTCA GAAGGAGGAG GAGGGAAAGG AATCAGAAAA 
GTCAACAATG CAGATGATTT CCCTAACCTC TTCAGACAGG TTCAAGCTGA AGTCCCTGGA 
TCTCCTATAT TTGTGATGAG ACTAGCCAAA CAATCTCGTC ATCTGGAGGT ACAGATCTTA 
GCAGATCAAT ATGGCAATGC TATCTCTCTC TTTGGTCGTG ACTGCTCTGT ACAGCGCAGG 
CATCAGAAGA TTATTGAGGA AGCACCTGCT GCTATTGCTA CTCCAGCAGT ATTTGAACAT 
ATGGAACAGT GTGCTGTGAA ACTTGCCAAA ATGGTTGGTT ATGTGAGTGC TGGGACTGTG 
GAATACCTCT ACAGCCAGGA TGGAAGCTTC TACTTTCTGG AACTGAACCC TCGGCTACAG 
GTGGAACACC CTTGTACAGA GATGGTGGCT GATGTCAACC TCCCTGCAGC ACAACTCCAG 
ATTGCCATGG GGATCCCTCT GTACAGAATC AAGGATATTC GTATGATGTA TGGGGTATCT 
CCCTGGGGTG ATGCTCCCAT TGATTTTGAA AATTCTGCTC ACGTTCCTTG CCCAAGGGGC 
CATGTTATTG CCGCTCGGAT CACTAGTGAA AATCCAGATG AGGGTTTTAA GCCCAGCTCA 
GGAACAGTTC AGGAACTGAA TTTCCGTAGC AATAAGAATG TTTGGGGTTA TTTCAGTGTT 
GCTGCTGCAG GGGGCCTTCA TGAATTTGCT GATTCTCAGT TTGGTCATTG CTTCTCCTGG 
GGAGAAAACA GAGAGGAAGC AATTTCAAAC ATGGTGGTGG CTTTGAAGGA GCTGTCTATT 
CGGGGTGACT TTCGAACCAC AGTTGAATAC CTCATCAAAT TGTTGGAGAC TGAAAGCTTT 
CAGTTGAATA GAATTGACAC CGGCTGGCTG GACAGACTGA TAGCAGAGAA AGTACAGGCA 
GAGCGACCTG ACACCATGTT GGGAGTTGTT TGTGGGGCTC TCCATGTGGC AGATGTGAGC 
CTACGGAATA GCATCTCTAA CTTCCTTCAT TCTTTAGAAA GGGGTCAAGT CCTTCCTGCT 
CATACACTTC TGAATACAGT AGATGTTGAA CTTATCTATG GGGGAGTCAA ATATGTACTT 
AAGGTGACTC GGCAGTCCCC CAACTCCTAT GTGGTGATCA TGAATGGTTC ATGCGTAGAA 
GTAGATGTGC ATCGGCTCAG TGATGGAGGG TTACTCCTGT CCTATGATGG CAGCAGTTAC 
ACCACATACA TGAAGGAAGA AGTGGATAGA TATCGTATCA CAATTGGCAA TAAGACCTGT 
GTGTTTGAGA AGGAAAATGA CCCTTCAGTG TTACGCTCAC CTTCTGCTGG GAAGTTAATC 
CAGTACATTG TGGAGGATGG AGGCCATGTG TTTGCTGGCC AGTGCTATGC TGAAATTGAG 
GTGATGAAGA TGGTAATGAC CTTAACAGCT GCAGAGTCTG GCTGTATCCA TTATGTCAAA 
CGGCCTGGAG CAGCTCTTGA CCCTGGCTGT GTAATAGCCA AAATGCAGTT GGACAACCCC 
AGCAAGGTCC AACAGGCTGA GCTTCACACA GGTAGTCTGC CACGGATCCA GAGCACTGCA 
CTCAGAGGCG AGAAGCTCCA TCGAGTGTTC CACTATGTCC TGGATAATCT GGTCAACGTA 
ATGAATGGAT ACTGCCTTCC AGATCCTTTT TTTAGCAGCA GGGTAAAAGA CTGGGTAGAG 
CGGTTGATGA AGACCCTCAG AGACCCCTCG TTACCTCTCC TAGAATTACA AGATATCATG 
ACCAGTGTCT CTGGCCGTAT TCCCCCCAAT GTTGAGAAGT CTATCAAGAA GGAAATGGCC 
CAGTATGCCA GCAATATCAC ATCAGTCCTC TGTCAGTTTC CCAGCCAGCA GATTGCCAAC 
ATCCTAGATA GCCATGCAGC TACGTTGAAC CGGAAATCTG AGCGGGAAGT CTTTTTCATG 
AACACTCAGA GCATTGTCCA GCTGGTACAG AGATACCGAA GTGGCATCCG AGGCCACATG 
AAGGCTGTGG TGATGGATCT GCTGCGGCAG TACCTGCGAG TAGAGACACA GTTCCAGAAT 
GGTCACTATG ACAAATGTGT GTTTGCCCTT CGAGAAGAGA ACAAGAGTGA TATGAACACT 
GTACTGAACT ATATCTTCTC TCATGCTCAA GTCACCAAGA AGAATCTTCT GGTCACAATG 
CTTATTGATC AGTTGTGCGG TAGGGACCCT ACTCTCACTG ATGAGCTGCT GAATATTCTC 
ACAGAGCTAA CTCAACTCAG TAAGACCACC AATGCCAAAG TAGCACTTCG AGCACGCCAG 
GTTCTTATTG CCTCCCATTT GCCATCATAT GAGCTTCGCC ATAACCAAGT AGAGTCTATC 
TTCCTTTCAG CTATTGACAT GTATGGACAT CAGTTTTGCA TTGAGAACCT GCAGAAACTC 
ATCTTGTCTG AAACATCTAT TTTTGATGTC CTACCAAACT TCTTCTATCA CAGCAACCAG 
GTAGTGAGGA TGGCAGCTCT GGAGGTTTAT GTTCGAAGGG CTTATATTGC CTATGAACTT 
AACAGCGTAC AACATCGCCA ACTTAAGGAC AACACCTGTG TGGTGGAATT CCAATTCATG 
CTACCCACAT CTCATCCAAA TAGAATGTCC TTCTCCTCCA ACCTCAACCA CTATGGCATG 
ACTCATGTAG CTAGTGTCAG CGATGTGCTT TTGGACAACT CATTCACTCC ACCATGTCAA 
CGGATGGGCG GAATGGTCTC TTTTCGGACT TTCGAAGATT TTGTCAGGAT CTTTGATGAA 
GTGATGGGCT GCTTCTGTGA TTCTCCACCG CAAAGCCCTA CATTCCCTGA GGCAGGTCAC 
ACATCTCTAT ACGATGAAGA CAAGGTCCCC AGGGATGAAC CAATTCACAT TCTGAATGTG 
GCTATCAAGA CTGACTGTGA TATTGAGGAT GACAGACTGG CAGCTATGTT CAGAGAGTTT 
ACCCAACAAA ATAAAGCTAC CCTGGTTGAG CATGGGATAC GGCGCCTTAC TTTCCTGGTT 
GCACAAAAGG ATTTCAGGAA ACAGGTCAAC TATGAGGTGG ATCAGAGATT TCATAGAGAA 
TTTCCTAAAT TTTTCACCTT CCGAGCAAGG GATAAGTTTG AGGAGGATCG TATCTATCGT 
CACCTGGAAC CTGCCCTCGC TTTCCAGTTA GAGCTGAACC GGATGAGAAA TTTTGACCTT 
ACTGCCATTC CATGTGCTAA TCATAAAATG CATCTGTATC TTGGGGCAGC CAAGGTGGAG 
GTGGGCACAG AAGTGACAGA CTACAGGTTC TTTGTTCGTG CAATCATCAG ACATTCTGAT 
CTGGTCACCA AGGAAGCTTC TTTTGAATAC CTACAAAATG AAGGGGAGAG GCTGCTTCTA 
GAAGCCATGG ATGAGTTGGA AGTTGCTTTT AACAATACAA ACGTTCGCAC TGACTGTAAC 
CACATCTTCC TCAACTTTGT GCCCACGGTC ATCATGGACC CATCAAAGAT TGAGGAATCT 
GTGCGGAGCA TGGTAATGCG CTATGGAAGT CGGCTGTGGA AATTACGTGT CCTCCAGGCA 
GAACTGAAAA TCAACATTCG CCTGACACCA ACTGGAAAAG CAATTCCCAT CCGCCTCTTC 
CTGACAAACG AGTCTGGCTA TTACTTGGAC ATCAGCTTGT ATAAGGAAGT GACTGACTCC 
AGGACAGCAC AGATCATGTT TCAGGCATAT GGAGACAAAC AGGGACCATT GCATGGAATG 
TTAATCAACA CTCCATACGT CACCAAAGAC CTGCTTCAAT CAAAGAGATT CCAGGCACAA 
TCCTTGGGGA CAACATACAT ATATGATATA CCAGAGATGT TTCGGCAGTC TCTGATCAAA 
CTCTGGGAGT CTATGTCCAC TCAAGCATTT CTTCCATCAC CCCCCCTGCC TTCCGACATG 
CTGACATATA CGGAGCTCGT GTTGGATGAT CAAGGTCAGC TGGTCCACAT GAACAGGCTT 
CCAGGAGGAA ATGAGATTGG CATGGTAGCG TGGAAAATGA CCCTTAAAAG TCCTGAATAC 
CCAGAAGGCC GAGATATCAT TGTTATTGGC AATGACATCA CATACCGAAT TGGGTCCTTT 
GGGCCCCAGG AGGATTTGCT GTTTCTCAGA GCTTCTGAGC TTGCCAGGGC AGAGGGTATC 
CCACGCATCT TTGTAGCAGC CAACAGTGGA GCAAGAATTG GACTGGCAGA GGAAATTCGT 
CATATGTTTC ATGTAGCCTG GGTAGATCCT GAGGATCCTT ACAAGGGATA CAAATATTTA 
TATCTGACCC CTCAGGATTA TAAGAGAGTC AGTGCTCTCA ACTCTGTCCA TTGTGAACAT 
GTAGAGGACG AAGGAGAATC CAGGTACAAG ATAACTGATA TTATTGGGAA GGAAGAGGGA 
CTTGGAGCAG AGAACCTTCG AGGCTCTGGA ATGATCGCTG GAGAATCCTC ATTGGCCTAT 
GATGAGATCA TCACCATCAG CCTGGTTACA TGCCGGGCCA TTGGGATTGG AGCTTACCTT 
GTCCGGCTGG GACAGAGAAC CATCCAGGTT GAAAATTCTC ATTTAATTTT GACTGGAGCT 
GGAGCCCTCA ACAAAGTCCT TGGGCGGGAA GTATACACCT CCAATAACCA GCTCGGGGGC 
ATCCAGATCA TGCACAACAA TGGGGTGACC CACAGCATTG TTTGTGATGA CTTTGAAGGG 
GTTTTCACTG TCCTGCACTG GCTATCCTAC ATGCCTAAGA ATGTGCGCAG TTTAGTTCCT 
CTCCTGAACT CCAAGGATCC TATAGATAGA ATCATCGAGT TTGTTCCCAC GAAGGCCCCA 
TACGATCCTC GATGGATGCT AGCAGGCCGC CCTCACCCAA CTCAGAAAGG TCAGTGGCTG 
AGTGGATTTT TTGACTATGG CTCTTTCTCA GAGATCATGC AACCCTGGGC ACAAACTGTG 
GTGGTTGGCA GAGCCAGGCT AGGAGGAATA CCTGTGGGAG TAGTTGCTGT AGAAACCCGA 
ACAGTGGAAC TAAGTATCCC AGCCGATCCT GCAAACCTGG ATTCTGAAGC CAAGATAATC 
CAGCAGGCTG GCCAGGTTTG GTTCCCAGAC TCTGCGTTTA AGACCTATCA GGCTATCAAG 
GACTTCAACC GTGAAGGACT GCCTCTGATG GTCTTTGCCA ACTGGAGAGG CTTCTCTGGT 
GGGATGAAAG ATATGTATGA CCAAGTGCTG AAGTTTGGTG CCTATATTGT GGATAGCTTA 
CGGGAGTGCT CCCAGCCTGT GCTGGTATAC ATTCCTCCTC AGGCTGAGCT CCGGGGTGGC 
TCCTGGGTTG TGATTGACCC CACCATCAAC CCCCGGCATA TGGAGATGTA CGCTGACCGA 
GAAAGCAGGG GATCAGTTCT GGAGCCAGAA GGGACAGTAG AAATCAAATT CCGCAGAAAG 
GATCTGGTGA AAACCATGCG TCGGGTGGAC CCAGTCTACA TTCGCTTGGC TGAGCGATTG 
GGCACCCCAG AGCTCAGCGC AGCTGAGCGG AAGGAGCTGG AGAACAAGTT GAAGGAGCGG 
GAGGAATTCC TAATTCCCAT TTACCATCAG GTAGCCGTGC AGTTTGCTGA CTTGCACGAC 
ACACCAGGCC GGATGCAGGA GAAGGGTGTT ATTAATGACA TCCTGGATTG GAAAACATCC 
CGTACCTTTT TCTATTGGCG GCTGAGGCGT CTCCTGCTGG AGGACCTGGT CAAAAAGAAA 
ATCCACAATG CCAACCCAGA GCTGACCGAT GGCCAGATCC AGGCCATGCT ACGGCGCTGG 
TTTGTAGAAG TGGAAGGAAC TGTGAAGGCC TACGTTTGGG ACAATAACAA GGACCTGGTG 
GAGTGGCTGG AGAAACAGCT GACCGAGGAA GATGGTGTTC GCTCAGTAAT CGAGGAAAAC 
ATCAAATATA TCAGCAGAGA CTATGTCCTC AAGCAGATCC GCAGCTTGGT ACAGGCCAAT 
CCCGAGGTTG CCATGGACTC CATCGTCCAT ATGACTCAGC ATATCTCACC CACTCAGCGA 
GCAGAAGTCG TACGGATCCT CTCCACGATG GACTCCCCTT CAACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_005624776.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624833.1 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X2, mRNA.

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CloneID	OCe12091
Clone ID Related Accession (Same CDS sequence)	XM_005624776.2 , XM_005624776.3
Accession Version	XM_005624776.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	acetyl-CoA carboxylase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624776.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGGT CCACTCTGAT GTCAATCTTG AGGGTTAGTT CTTTTTGGAG GTGGATATCT 
GCTCAGACAG TGAGAATTAT AAGAGCTTTG AGAGCTCATT TTGAAAGAAT AATGGATGAA 
CCATCCCCCT TGGCCAAACC TCTGGAGCTG AACCAGCACT CCCGATTCAT AATTGGTTCT 
GTGTCTGAAG ATAACTCAGA GGATGAAATT AGCAACCTGG TGAAGCTGGA TCTCCTGGAG 
GAGAAGGAGG GCTCTCTATC ACCAGCTTCT GTTGGCTCAG ATACACTTTC TGACTTGGGG 
ATCTCCAGCC TACAGGACGG CTTGGCCTTA CACATGAGGT CCAGCATGTC TGGCTTGCAT 
CTAGTGAAGC AAGGTCGAGA CCGAAAGAAA ATAGACTCTC AGCGAGATTT CACTGTAGCT 
TCTCCAGCAG AATTTGTTAC TCGTTTTGGG GGAAATAAAG TGATTGAGAA GGTTCTTATT 
GCCAACAATG GCATTGCAGC AGTGAAATGC ATGCGGTCTA TCCGTCGATG GTCTTATGAG 
ATGTTTCGAA ATGAACGTGC AATCCGATTT GTTGTTATGG TAACACCTGA AGACCTGAAA 
GCCAATGCAG AATACATTAA GATGGCGGAT CACTATGTGC CAGTGCCAGG AGGACCAAAT 
AACAACAACT ATGCGAACGT GGAATTAATT CTTGATATTG CTAAAAGGAT TCCCGTACAA 
GCAGTGTGGG CTGGCTGGGG TCATGCTTCT GAGAATCCCA AGCTCCCAGA ACTTCTTTTG 
AAAAATGGCA TCGCCTTCAT GGGCCCTCCA AGCCAGGCCA TGTGGGCTTT GGGGGATAAG 
ATTGCATCTT CTATAGTGGC TCAAACTGCA GGTATCCCAA CTCTTCCCTG GAGTGGCAGT 
GGTCTTCGGG TGGACTGGCA TGAAAATGAT TTTTCCAAAC GTATCTTAAA TGTTCCCCAG 
GAATTATACG AAAAAGGTTA TGTGAAGGAT GTGGATGATG GGCTAAAGGC AGCGGAGGAA 
GTTGGATATC CAGTAATGAT CAAGGCCTCA GAAGGAGGAG GAGGGAAAGG AATCAGAAAA 
GTCAACAATG CAGATGATTT CCCTAACCTC TTCAGACAGG TTCAAGCTGA AGTCCCTGGA 
TCTCCTATAT TTGTGATGAG ACTAGCCAAA CAATCTCGTC ATCTGGAGGT ACAGATCTTA 
GCAGATCAAT ATGGCAATGC TATCTCTCTC TTTGGTCGTG ACTGCTCTGT ACAGCGCAGG 
CATCAGAAGA TTATTGAGGA AGCACCTGCT GCTATTGCTA CTCCAGCAGT ATTTGAACAT 
ATGGAACAGT GTGCTGTGAA ACTTGCCAAA ATGGTTGGTT ATGTGAGTGC TGGGACTGTG 
GAATACCTCT ACAGCCAGGA TGGAAGCTTC TACTTTCTGG AACTGAACCC TCGGCTACAG 
GTGGAACACC CTTGTACAGA GATGGTGGCT GATGTCAACC TCCCTGCAGC ACAACTCCAG 
ATTGCCATGG GGATCCCTCT GTACAGAATC AAGGATATTC GTATGATGTA TGGGGTATCT 
CCCTGGGGTG ATGCTCCCAT TGATTTTGAA AATTCTGCTC ACGTTCCTTG CCCAAGGGGC 
CATGTTATTG CCGCTCGGAT CACTAGTGAA AATCCAGATG AGGGTTTTAA GCCCAGCTCA 
GGAACAGTTC AGGAACTGAA TTTCCGTAGC AATAAGAATG TTTGGGGTTA TTTCAGTGTT 
GCTGCTGCAG GGGGCCTTCA TGAATTTGCT GATTCTCAGT TTGGTCATTG CTTCTCCTGG 
GGAGAAAACA GAGAGGAAGC AATTTCAAAC ATGGTGGTGG CTTTGAAGGA GCTGTCTATT 
CGGGGTGACT TTCGAACCAC AGTTGAATAC CTCATCAAAT TGTTGGAGAC TGAAAGCTTT 
CAGTTGAATA GAATTGACAC CGGCTGGCTG GACAGACTGA TAGCAGAGAA AGTACAGGCA 
GAGCGACCTG ACACCATGTT GGGAGTTGTT TGTGGGGCTC TCCATGTGGC AGATGTGAGC 
CTACGGAATA GCATCTCTAA CTTCCTTCAT TCTTTAGAAA GGGGTCAAGT CCTTCCTGCT 
CATACACTTC TGAATACAGT AGATGTTGAA CTTATCTATG GGGGAGTCAA ATATGTACTT 
AAGGTGACTC GGCAGTCCCC CAACTCCTAT GTGGTGATCA TGAATGGTTC ATGCGTAGAA 
GTAGATGTGC ATCGGCTCAG TGATGGAGGG TTACTCCTGT CCTATGATGG CAGCAGTTAC 
ACCACATACA TGAAGGAAGA AGTGGATAGA TATCGTATCA CAATTGGCAA TAAGACCTGT 
GTGTTTGAGA AGGAAAATGA CCCTTCAGTG TTACGCTCAC CTTCTGCTGG GAAGTTAATC 
CAGTACATTG TGGAGGATGG AGGCCATGTG TTTGCTGGCC AGTGCTATGC TGAAATTGAG 
GTGATGAAGA TGGTAATGAC CTTAACAGCT GCAGAGTCTG GCTGTATCCA TTATGTCAAA 
CGGCCTGGAG CAGCTCTTGA CCCTGGCTGT GTAATAGCCA AAATGCAGTT GGACAACCCC 
AGCAAGGTCC AACAGGCTGA GCTTCACACA GGTAGTCTGC CACGGATCCA GAGCACTGCA 
CTCAGAGGCG AGAAGCTCCA TCGAGTGTTC CACTATGTCC TGGATAATCT GGTCAACGTA 
ATGAATGGAT ACTGCCTTCC AGATCCTTTT TTTAGCAGCA GGGTAAAAGA CTGGGTAGAG 
CGGTTGATGA AGACCCTCAG AGACCCCTCG TTACCTCTCC TAGAATTACA AGATATCATG 
ACCAGTGTCT CTGGCCGTAT TCCCCCCAAT GTTGAGAAGT CTATCAAGAA GGAAATGGCC 
CAGTATGCCA GCAATATCAC ATCAGTCCTC TGTCAGTTTC CCAGCCAGCA GATTGCCAAC 
ATCCTAGATA GCCATGCAGC TACGTTGAAC CGGAAATCTG AGCGGGAAGT CTTTTTCATG 
AACACTCAGA GCATTGTCCA GCTGGTACAG AGATACCGAA GTGGCATCCG AGGCCACATG 
AAGGCTGTGG TGATGGATCT GCTGCGGCAG TACCTGCGAG TAGAGACACA GTTCCAGAAT 
GGTCACTATG ACAAATGTGT GTTTGCCCTT CGAGAAGAGA ACAAGAGTGA TATGAACACT 
GTACTGAACT ATATCTTCTC TCATGCTCAA GTCACCAAGA AGAATCTTCT GGTCACAATG 
CTTATTGATC AGTTGTGCGG TAGGGACCCT ACTCTCACTG ATGAGCTGCT GAATATTCTC 
ACAGAGCTAA CTCAACTCAG TAAGACCACC AATGCCAAAG TAGCACTTCG AGCACGCCAG 
GTTCTTATTG CCTCCCATTT GCCATCATAT GAGCTTCGCC ATAACCAAGT AGAGTCTATC 
TTCCTTTCAG CTATTGACAT GTATGGACAT CAGTTTTGCA TTGAGAACCT GCAGAAACTC 
ATCTTGTCTG AAACATCTAT TTTTGATGTC CTACCAAACT TCTTCTATCA CAGCAACCAG 
GTAGTGAGGA TGGCAGCTCT GGAGGTTTAT GTTCGAAGGG CTTATATTGC CTATGAACTT 
AACAGCGTAC AACATCGCCA ACTTAAGGAC AACACCTGTG TGGTGGAATT CCAATTCATG 
CTACCCACAT CTCATCCAAA TAGAATGTCC TTCTCCTCCA ACCTCAACCA CTATGGCATG 
ACTCATGTAG CTAGTGTCAG CGATGTGCTT TTGGACAACT CATTCACTCC ACCATGTCAA 
CGGATGGGCG GAATGGTCTC TTTTCGGACT TTCGAAGATT TTGTCAGGAT CTTTGATGAA 
GTGATGGGCT GCTTCTGTGA TTCTCCACCG CAAAGCCCTA CATTCCCTGA GGCAGGTCAC 
ACATCTCTAT ACGATGAAGA CAAGGTCCCC AGGGATGAAC CAATTCACAT TCTGAATGTG 
GCTATCAAGA CTGACTGTGA TATTGAGGAT GACAGACTGG CAGCTATGTT CAGAGAGTTT 
ACCCAACAAA ATAAAGCTAC CCTGGTTGAG CATGGGATAC GGCGCCTTAC TTTCCTGGTT 
GCACAAAAGG ATTTCAGGAA ACAGGTCAAC TATGAGGTGG ATCAGAGATT TCATAGAGAA 
TTTCCTAAAT TTTTCACCTT CCGAGCAAGG GATAAGTTTG AGGAGGATCG TATCTATCGT 
CACCTGGAAC CTGCCCTCGC TTTCCAGTTA GAGCTGAACC GGATGAGAAA TTTTGACCTT 
ACTGCCATTC CATGTGCTAA TCATAAAATG CATCTGTATC TTGGGGCAGC CAAGGTGGAG 
GTGGGCACAG AAGTGACAGA CTACAGGTTC TTTGTTCGTG CAATCATCAG ACATTCTGAT 
CTGGTCACCA AGGAAGCTTC TTTTGAATAC CTACAAAATG AAGGGGAGAG GCTGCTTCTA 
GAAGCCATGG ATGAGTTGGA AGTTGCTTTT AACAATACAA ACGTTCGCAC TGACTGTAAC 
CACATCTTCC TCAACTTTGT GCCCACGGTC ATCATGGACC CATCAAAGAT TGAGGAATCT 
GTGCGGAGCA TGGTAATGCG CTATGGAAGT CGGCTGTGGA AATTACGTGT CCTCCAGGCA 
GAACTGAAAA TCAACATTCG CCTGACACCA ACTGGAAAAG CAATTCCCAT CCGCCTCTTC 
CTGACAAACG AGTCTGGCTA TTACTTGGAC ATCAGCTTGT ATAAGGAAGT GACTGACTCC 
AGGACAGCAC AGATCATGTT TCAGGCATAT GGAGACAAAC AGGGACCATT GCATGGAATG 
TTAATCAACA CTCCATACGT CACCAAAGAC CTGCTTCAAT CAAAGAGATT CCAGGCACAA 
TCCTTGGGGA CAACATACAT ATATGATATA CCAGAGATGT TTCGGCAGTC TCTGATCAAA 
CTCTGGGAGT CTATGTCCAC TCAAGCATTT CTTCCATCAC CCCCCCTGCC TTCCGACATG 
CTGACATATA CGGAGCTCGT GTTGGATGAT CAAGGTCAGC TGGTCCACAT GAACAGGCTT 
CCAGGAGGAA ATGAGATTGG CATGGTAGCG TGGAAAATGA CCCTTAAAAG TCCTGAATAC 
CCAGAAGGCC GAGATATCAT TGTTATTGGC AATGACATCA CATACCGAAT TGGGTCCTTT 
GGGCCCCAGG AGGATTTGCT GTTTCTCAGA GCTTCTGAGC TTGCCAGGGC AGAGGGTATC 
CCACGCATCT TTGTAGCAGC CAACAGTGGA GCAAGAATTG GACTGGCAGA GGAAATTCGT 
CATATGTTTC ATGTAGCCTG GGTAGATCCT GAGGATCCTT ACAAGGGATA CAAATATTTA 
TATCTGACCC CTCAGGATTA TAAGAGAGTC AGTGCTCTCA ACTCTGTCCA TTGTGAACAT 
GTAGAGGACG AAGGAGAATC CAGGTACAAG ATAACTGATA TTATTGGGAA GGAAGAGGGA 
CTTGGAGCAG AGAACCTTCG AGGCTCTGGA ATGATCGCTG GAGAATCCTC ATTGGCCTAT 
GATGAGATCA TCACCATCAG CCTGGTTACA TGCCGGGCCA TTGGGATTGG AGCTTACCTT 
GTCCGGCTGG GACAGAGAAC CATCCAGGTT GAAAATTCTC ATTTAATTTT GACTGGAGCT 
GGAGCCCTCA ACAAAGTCCT TGGGCGGGAA GTATACACCT CCAATAACCA GCTCGGGGGC 
ATCCAGATCA TGCACAACAA TGGGGTGACC CACAGCATTG TTTGTGATGA CTTTGAAGGG 
GTTTTCACTG TCCTGCACTG GCTATCCTAC ATGCCTAAGA ATGTGCGCAG TTTAGTTCCT 
CTCCTGAACT CCAAGGATCC TATAGATAGA ATCATCGAGT TTGTTCCCAC GAAGGCCCCA 
TACGATCCTC GATGGATGCT AGCAGGCCGC CCTCACCCAA CTCAGAAAGG TCAGTGGCTG 
AGTGGATTTT TTGACTATGG CTCTTTCTCA GAGATCATGC AACCCTGGGC ACAAACTGTG 
GTGGTTGGCA GAGCCAGGCT AGGAGGAATA CCTGTGGGAG TAGTTGCTGT AGAAACCCGA 
ACAGTGGAAC TAAGTATCCC AGCCGATCCT GCAAACCTGG ATTCTGAAGC CAAGATAATC 
CAGCAGGCTG GCCAGGTTTG GTTCCCAGAC TCTGCGTTTA AGACCTATCA GGCTATCAAG 
GACTTCAACC GTGAAGGACT GCCTCTGATG GTCTTTGCCA ACTGGAGAGG CTTCTCTGGT 
GGGATGAAAG ATATGTATGA CCAAGTGCTG AAGTTTGGTG CCTATATTGT GGATAGCTTA 
CGGGAGTGCT CCCAGCCTGT GCTGGTATAC ATTCCTCCTC AGGCTGAGCT CCGGGGTGGC 
TCCTGGGTTG TGATTGACCC CACCATCAAC CCCCGGCATA TGGAGATGTA CGCTGACCGA 
GAAAGCAGGG GATCAGTTCT GGAGCCAGAA GGGACAGTAG AAATCAAATT CCGCAGAAAG 
GATCTGGTGA AAACCATGCG TCGGGTGGAC CCAGTCTACA TTCGCTTGGC TGAGCGATTG 
GGCACCCCAG AGCTCAGCGC AGCTGAGCGG AAGGAGCTGG AGAACAAGTT GAAGGAGCGG 
GAGGAATTCC TAATTCCCAT TTACCATCAG GTAGCCGTGC AGTTTGCTGA CTTGCACGAC 
ACACCAGGCC GGATGCAGGA GAAGGGTGTT ATTAATGACA TCCTGGATTG GAAAACATCC 
CGTACCTTTT TCTATTGGCG GCTGAGGCGT CTCCTGCTGG AGGACCTGGT CAAAAAGAAA 
ATCCACAATG CCAACCCAGA GCTGACCGAT GGCCAGATCC AGGCCATGCT ACGGCGCTGG 
TTTGTAGAAG TGGAAGGAAC TGTGAAGGCC TACGTTTGGG ACAATAACAA GGACCTGGTG 
GAGTGGCTGG AGAAACAGCT GACCGAGGAA GATGGTGTTC GCTCAGTAAT CGAGGAAAAC 
ATCAAATATA TCAGCAGAGA CTATGTCCTC AAGCAGATCC GCAGCTTGGT ACAGGCCAAT 
CCCGAGGTTG CCATGGACTC CATCGTCCAT ATGACTCAGC ATATCTCACC CACTCAGCGA 
GCAGAAGTCG TACGGATCCT CTCCACGATG GACTCCCCTT CAACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624833.1
CDS	424..7551
Translation	MWWSTLMSILRVSSFWRWISAQTVRIIRALRAHFERIMDEPSPLAKPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_005624776.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624776.3

ORF Insert Sequence:

ATGTGGTGGT CCACTCTGAT GTCAATCTTG AGGGTTAGTT CTTTTTGGAG GTGGATATCT 
GCTCAGACAG TGAGAATTAT AAGAGCTTTG AGAGCTCATT TTGAAAGAAT AATGGATGAA 
CCATCCCCCT TGGCCAAACC TCTGGAGCTG AACCAGCACT CCCGATTCAT AATTGGTTCT 
GTGTCTGAAG ATAACTCAGA GGATGAAATT AGCAACCTGG TGAAGCTGGA TCTCCTGGAG 
GAGAAGGAGG GCTCTCTATC ACCAGCTTCT GTTGGCTCAG ATACACTTTC TGACTTGGGG 
ATCTCCAGCC TACAGGACGG CTTGGCCTTA CACATGAGGT CCAGCATGTC TGGCTTGCAT 
CTAGTGAAGC AAGGTCGAGA CCGAAAGAAA ATAGACTCTC AGCGAGATTT CACTGTAGCT 
TCTCCAGCAG AATTTGTTAC TCGTTTTGGG GGAAATAAAG TGATTGAGAA GGTTCTTATT 
GCCAACAATG GCATTGCAGC AGTGAAATGC ATGCGGTCTA TCCGTCGATG GTCTTATGAG 
ATGTTTCGAA ATGAACGTGC AATCCGATTT GTTGTTATGG TAACACCTGA AGACCTGAAA 
GCCAATGCAG AATACATTAA GATGGCGGAT CACTATGTGC CAGTGCCAGG AGGACCAAAT 
AACAACAACT ATGCGAACGT GGAATTAATT CTTGATATTG CTAAAAGGAT TCCCGTACAA 
GCAGTGTGGG CTGGCTGGGG TCATGCTTCT GAGAATCCCA AGCTCCCAGA ACTTCTTTTG 
AAAAATGGCA TCGCCTTCAT GGGCCCTCCA AGCCAGGCCA TGTGGGCTTT GGGGGATAAG 
ATTGCATCTT CTATAGTGGC TCAAACTGCA GGTATCCCAA CTCTTCCCTG GAGTGGCAGT 
GGTCTTCGGG TGGACTGGCA TGAAAATGAT TTTTCCAAAC GTATCTTAAA TGTTCCCCAG 
GAATTATACG AAAAAGGTTA TGTGAAGGAT GTGGATGATG GGCTAAAGGC AGCGGAGGAA 
GTTGGATATC CAGTAATGAT CAAGGCCTCA GAAGGAGGAG GAGGGAAAGG AATCAGAAAA 
GTCAACAATG CAGATGATTT CCCTAACCTC TTCAGACAGG TTCAAGCTGA AGTCCCTGGA 
TCTCCTATAT TTGTGATGAG ACTAGCCAAA CAATCTCGTC ATCTGGAGGT ACAGATCTTA 
GCAGATCAAT ATGGCAATGC TATCTCTCTC TTTGGTCGTG ACTGCTCTGT ACAGCGCAGG 
CATCAGAAGA TTATTGAGGA AGCACCTGCT GCTATTGCTA CTCCAGCAGT ATTTGAACAT 
ATGGAACAGT GTGCTGTGAA ACTTGCCAAA ATGGTTGGTT ATGTGAGTGC TGGGACTGTG 
GAATACCTCT ACAGCCAGGA TGGAAGCTTC TACTTTCTGG AACTGAACCC TCGGCTACAG 
GTGGAACACC CTTGTACAGA GATGGTGGCT GATGTCAACC TCCCTGCAGC ACAACTCCAG 
ATTGCCATGG GGATCCCTCT GTACAGAATC AAGGATATTC GTATGATGTA TGGGGTATCT 
CCCTGGGGTG ATGCTCCCAT TGATTTTGAA AATTCTGCTC ACGTTCCTTG CCCAAGGGGC 
CATGTTATTG CCGCTCGGAT CACTAGTGAA AATCCAGATG AGGGTTTTAA GCCCAGCTCA 
GGAACAGTTC AGGAACTGAA TTTCCGTAGC AATAAGAATG TTTGGGGTTA TTTCAGTGTT 
GCTGCTGCAG GGGGCCTTCA TGAATTTGCT GATTCTCAGT TTGGTCATTG CTTCTCCTGG 
GGAGAAAACA GAGAGGAAGC AATTTCAAAC ATGGTGGTGG CTTTGAAGGA GCTGTCTATT 
CGGGGTGACT TTCGAACCAC AGTTGAATAC CTCATCAAAT TGTTGGAGAC TGAAAGCTTT 
CAGTTGAATA GAATTGACAC CGGCTGGCTG GACAGACTGA TAGCAGAGAA AGTACAGGCA 
GAGCGACCTG ACACCATGTT GGGAGTTGTT TGTGGGGCTC TCCATGTGGC AGATGTGAGC 
CTACGGAATA GCATCTCTAA CTTCCTTCAT TCTTTAGAAA GGGGTCAAGT CCTTCCTGCT 
CATACACTTC TGAATACAGT AGATGTTGAA CTTATCTATG GGGGAGTCAA ATATGTACTT 
AAGGTGACTC GGCAGTCCCC CAACTCCTAT GTGGTGATCA TGAATGGTTC ATGCGTAGAA 
GTAGATGTGC ATCGGCTCAG TGATGGAGGG TTACTCCTGT CCTATGATGG CAGCAGTTAC 
ACCACATACA TGAAGGAAGA AGTGGATAGA TATCGTATCA CAATTGGCAA TAAGACCTGT 
GTGTTTGAGA AGGAAAATGA CCCTTCAGTG TTACGCTCAC CTTCTGCTGG GAAGTTAATC 
CAGTACATTG TGGAGGATGG AGGCCATGTG TTTGCTGGCC AGTGCTATGC TGAAATTGAG 
GTGATGAAGA TGGTAATGAC CTTAACAGCT GCAGAGTCTG GCTGTATCCA TTATGTCAAA 
CGGCCTGGAG CAGCTCTTGA CCCTGGCTGT GTAATAGCCA AAATGCAGTT GGACAACCCC 
AGCAAGGTCC AACAGGCTGA GCTTCACACA GGTAGTCTGC CACGGATCCA GAGCACTGCA 
CTCAGAGGCG AGAAGCTCCA TCGAGTGTTC CACTATGTCC TGGATAATCT GGTCAACGTA 
ATGAATGGAT ACTGCCTTCC AGATCCTTTT TTTAGCAGCA GGGTAAAAGA CTGGGTAGAG 
CGGTTGATGA AGACCCTCAG AGACCCCTCG TTACCTCTCC TAGAATTACA AGATATCATG 
ACCAGTGTCT CTGGCCGTAT TCCCCCCAAT GTTGAGAAGT CTATCAAGAA GGAAATGGCC 
CAGTATGCCA GCAATATCAC ATCAGTCCTC TGTCAGTTTC CCAGCCAGCA GATTGCCAAC 
ATCCTAGATA GCCATGCAGC TACGTTGAAC CGGAAATCTG AGCGGGAAGT CTTTTTCATG 
AACACTCAGA GCATTGTCCA GCTGGTACAG AGATACCGAA GTGGCATCCG AGGCCACATG 
AAGGCTGTGG TGATGGATCT GCTGCGGCAG TACCTGCGAG TAGAGACACA GTTCCAGAAT 
GGTCACTATG ACAAATGTGT GTTTGCCCTT CGAGAAGAGA ACAAGAGTGA TATGAACACT 
GTACTGAACT ATATCTTCTC TCATGCTCAA GTCACCAAGA AGAATCTTCT GGTCACAATG 
CTTATTGATC AGTTGTGCGG TAGGGACCCT ACTCTCACTG ATGAGCTGCT GAATATTCTC 
ACAGAGCTAA CTCAACTCAG TAAGACCACC AATGCCAAAG TAGCACTTCG AGCACGCCAG 
GTTCTTATTG CCTCCCATTT GCCATCATAT GAGCTTCGCC ATAACCAAGT AGAGTCTATC 
TTCCTTTCAG CTATTGACAT GTATGGACAT CAGTTTTGCA TTGAGAACCT GCAGAAACTC 
ATCTTGTCTG AAACATCTAT TTTTGATGTC CTACCAAACT TCTTCTATCA CAGCAACCAG 
GTAGTGAGGA TGGCAGCTCT GGAGGTTTAT GTTCGAAGGG CTTATATTGC CTATGAACTT 
AACAGCGTAC AACATCGCCA ACTTAAGGAC AACACCTGTG TGGTGGAATT CCAATTCATG 
CTACCCACAT CTCATCCAAA TAGAATGTCC TTCTCCTCCA ACCTCAACCA CTATGGCATG 
ACTCATGTAG CTAGTGTCAG CGATGTGCTT TTGGACAACT CATTCACTCC ACCATGTCAA 
CGGATGGGCG GAATGGTCTC TTTTCGGACT TTCGAAGATT TTGTCAGGAT CTTTGATGAA 
GTGATGGGCT GCTTCTGTGA TTCTCCACCG CAAAGCCCTA CATTCCCTGA GGCAGGTCAC 
ACATCTCTAT ACGATGAAGA CAAGGTCCCC AGGGATGAAC CAATTCACAT TCTGAATGTG 
GCTATCAAGA CTGACTGTGA TATTGAGGAT GACAGACTGG CAGCTATGTT CAGAGAGTTT 
ACCCAACAAA ATAAAGCTAC CCTGGTTGAG CATGGGATAC GGCGCCTTAC TTTCCTGGTT 
GCACAAAAGG ATTTCAGGAA ACAGGTCAAC TATGAGGTGG ATCAGAGATT TCATAGAGAA 
TTTCCTAAAT TTTTCACCTT CCGAGCAAGG GATAAGTTTG AGGAGGATCG TATCTATCGT 
CACCTGGAAC CTGCCCTCGC TTTCCAGTTA GAGCTGAACC GGATGAGAAA TTTTGACCTT 
ACTGCCATTC CATGTGCTAA TCATAAAATG CATCTGTATC TTGGGGCAGC CAAGGTGGAG 
GTGGGCACAG AAGTGACAGA CTACAGGTTC TTTGTTCGTG CAATCATCAG ACATTCTGAT 
CTGGTCACCA AGGAAGCTTC TTTTGAATAC CTACAAAATG AAGGGGAGAG GCTGCTTCTA 
GAAGCCATGG ATGAGTTGGA AGTTGCTTTT AACAATACAA ACGTTCGCAC TGACTGTAAC 
CACATCTTCC TCAACTTTGT GCCCACGGTC ATCATGGACC CATCAAAGAT TGAGGAATCT 
GTGCGGAGCA TGGTAATGCG CTATGGAAGT CGGCTGTGGA AATTACGTGT CCTCCAGGCA 
GAACTGAAAA TCAACATTCG CCTGACACCA ACTGGAAAAG CAATTCCCAT CCGCCTCTTC 
CTGACAAACG AGTCTGGCTA TTACTTGGAC ATCAGCTTGT ATAAGGAAGT GACTGACTCC 
AGGACAGCAC AGATCATGTT TCAGGCATAT GGAGACAAAC AGGGACCATT GCATGGAATG 
TTAATCAACA CTCCATACGT CACCAAAGAC CTGCTTCAAT CAAAGAGATT CCAGGCACAA 
TCCTTGGGGA CAACATACAT ATATGATATA CCAGAGATGT TTCGGCAGTC TCTGATCAAA 
CTCTGGGAGT CTATGTCCAC TCAAGCATTT CTTCCATCAC CCCCCCTGCC TTCCGACATG 
CTGACATATA CGGAGCTCGT GTTGGATGAT CAAGGTCAGC TGGTCCACAT GAACAGGCTT 
CCAGGAGGAA ATGAGATTGG CATGGTAGCG TGGAAAATGA CCCTTAAAAG TCCTGAATAC 
CCAGAAGGCC GAGATATCAT TGTTATTGGC AATGACATCA CATACCGAAT TGGGTCCTTT 
GGGCCCCAGG AGGATTTGCT GTTTCTCAGA GCTTCTGAGC TTGCCAGGGC AGAGGGTATC 
CCACGCATCT TTGTAGCAGC CAACAGTGGA GCAAGAATTG GACTGGCAGA GGAAATTCGT 
CATATGTTTC ATGTAGCCTG GGTAGATCCT GAGGATCCTT ACAAGGGATA CAAATATTTA 
TATCTGACCC CTCAGGATTA TAAGAGAGTC AGTGCTCTCA ACTCTGTCCA TTGTGAACAT 
GTAGAGGACG AAGGAGAATC CAGGTACAAG ATAACTGATA TTATTGGGAA GGAAGAGGGA 
CTTGGAGCAG AGAACCTTCG AGGCTCTGGA ATGATCGCTG GAGAATCCTC ATTGGCCTAT 
GATGAGATCA TCACCATCAG CCTGGTTACA TGCCGGGCCA TTGGGATTGG AGCTTACCTT 
GTCCGGCTGG GACAGAGAAC CATCCAGGTT GAAAATTCTC ATTTAATTTT GACTGGAGCT 
GGAGCCCTCA ACAAAGTCCT TGGGCGGGAA GTATACACCT CCAATAACCA GCTCGGGGGC 
ATCCAGATCA TGCACAACAA TGGGGTGACC CACAGCATTG TTTGTGATGA CTTTGAAGGG 
GTTTTCACTG TCCTGCACTG GCTATCCTAC ATGCCTAAGA ATGTGCGCAG TTTAGTTCCT 
CTCCTGAACT CCAAGGATCC TATAGATAGA ATCATCGAGT TTGTTCCCAC GAAGGCCCCA 
TACGATCCTC GATGGATGCT AGCAGGCCGC CCTCACCCAA CTCAGAAAGG TCAGTGGCTG 
AGTGGATTTT TTGACTATGG CTCTTTCTCA GAGATCATGC AACCCTGGGC ACAAACTGTG 
GTGGTTGGCA GAGCCAGGCT AGGAGGAATA CCTGTGGGAG TAGTTGCTGT AGAAACCCGA 
ACAGTGGAAC TAAGTATCCC AGCCGATCCT GCAAACCTGG ATTCTGAAGC CAAGATAATC 
CAGCAGGCTG GCCAGGTTTG GTTCCCAGAC TCTGCGTTTA AGACCTATCA GGCTATCAAG 
GACTTCAACC GTGAAGGACT GCCTCTGATG GTCTTTGCCA ACTGGAGAGG CTTCTCTGGT 
GGGATGAAAG ATATGTATGA CCAAGTGCTG AAGTTTGGTG CCTATATTGT GGATAGCTTA 
CGGGAGTGCT CCCAGCCTGT GCTGGTATAC ATTCCTCCTC AGGCTGAGCT CCGGGGTGGC 
TCCTGGGTTG TGATTGACCC CACCATCAAC CCCCGGCATA TGGAGATGTA CGCTGACCGA 
GAAAGCAGGG GATCAGTTCT GGAGCCAGAA GGGACAGTAG AAATCAAATT CCGCAGAAAG 
GATCTGGTGA AAACCATGCG TCGGGTGGAC CCAGTCTACA TTCGCTTGGC TGAGCGATTG 
GGCACCCCAG AGCTCAGCGC AGCTGAGCGG AAGGAGCTGG AGAACAAGTT GAAGGAGCGG 
GAGGAATTCC TAATTCCCAT TTACCATCAG GTAGCCGTGC AGTTTGCTGA CTTGCACGAC 
ACACCAGGCC GGATGCAGGA GAAGGGTGTT ATTAATGACA TCCTGGATTG GAAAACATCC 
CGTACCTTTT TCTATTGGCG GCTGAGGCGT CTCCTGCTGG AGGACCTGGT CAAAAAGAAA 
ATCCACAATG CCAACCCAGA GCTGACCGAT GGCCAGATCC AGGCCATGCT ACGGCGCTGG 
TTTGTAGAAG TGGAAGGAAC TGTGAAGGCC TACGTTTGGG ACAATAACAA GGACCTGGTG 
GAGTGGCTGG AGAAACAGCT GACCGAGGAA GATGGTGTTC GCTCAGTAAT CGAGGAAAAC 
ATCAAATATA TCAGCAGAGA CTATGTCCTC AAGCAGATCC GCAGCTTGGT ACAGGCCAAT 
CCCGAGGTTG CCATGGACTC CATCGTCCAT ATGACTCAGC ATATCTCACC CACTCAGCGA 
GCAGAAGTCG TACGGATCCT CTCCACGATG GACTCCCCTT CAACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_005624777.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624834.1 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X3, mRNA.

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CloneID	OCe12092
Clone ID Related Accession (Same CDS sequence)	XM_005624777.3 , XM_005624777.2
Accession Version	XM_005624777.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	acetyl-CoA carboxylase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624777.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGGT CCACTCTGAT GTCAATCTTG AGGGTTAGTT CTTTTTGGAG GTGGATATCT 
GCTCAGACAG TGAGAATTAT AAGAGCTTTG AGAGCTCATT TTGAAAGAAT AATGGATGAA 
CCATCCCCCT TGGCCAAACC TCTGGAGCTG AACCAGCACT CCCGATTCAT AATTGGTTCT 
GTGTCTGAAG ATAACTCAGA GGATGAAATT AGCAACCTGG TGAAGCTGGA TCTCCTGGAG 
GAGAAGGAGG GCTCTCTATC ACCAGCTTCT GTTGGCTCAG ATACACTTTC TGACTTGGGG 
ATCTCCAGCC TACAGGACGG CTTGGCCTTA CACATGAGGT CCAGCATGTC TGGCTTGCAT 
CTAGTGAAGC AAGGTCGAGA CCGAAAGAAA ATAGACTCTC AGCGAGATTT CACTGTAGCT 
TCTCCAGCAG AATTTGTTAC TCGTTTTGGG GGAAATAAAG TGATTGAGAA GGTTCTTATT 
GCCAACAATG GCATTGCAGC AGTGAAATGC ATGCGGTCTA TCCGTCGATG GTCTTATGAG 
ATGTTTCGAA ATGAACGTGC AATCCGATTT GTTGTTATGG TAACACCTGA AGACCTGAAA 
GCCAATGCAG AATACATTAA GATGGCGGAT CACTATGTGC CAGTGCCAGG AGGACCAAAT 
AACAACAACT ATGCGAACGT GGAATTAATT CTTGATATTG CTAAAAGGAT TCCCGTACAA 
GCAGTGTGGG CTGGCTGGGG TCATGCTTCT GAGAATCCCA AGCTCCCAGA ACTTCTTTTG 
AAAAATGGCA TCGCCTTCAT GGGCCCTCCA AGCCAGGCCA TGTGGGCTTT GGGGGATAAG 
ATTGCATCTT CTATAGTGGC TCAAACTGCA GGTATCCCAA CTCTTCCCTG GAGTGGCAGT 
GGTCTTCGGG TGGACTGGCA TGAAAATGAT TTTTCCAAAC GTATCTTAAA TGTTCCCCAG 
GAATTATACG AAAAAGGTTA TGTGAAGGAT GTGGATGATG GGCTAAAGGC AGCGGAGGAA 
GTTGGATATC CAGTAATGAT CAAGGCCTCA GAAGGAGGAG GAGGGAAAGG AATCAGAAAA 
GTCAACAATG CAGATGATTT CCCTAACCTC TTCAGACAGG TTCAAGCTGA AGTCCCTGGA 
TCTCCTATAT TTGTGATGAG ACTAGCCAAA CAATCTCGTC ATCTGGAGGT ACAGATCTTA 
GCAGATCAAT ATGGCAATGC TATCTCTCTC TTTGGTCGTG ACTGCTCTGT ACAGCGCAGG 
CATCAGAAGA TTATTGAGGA AGCACCTGCT GCTATTGCTA CTCCAGCAGT ATTTGAACAT 
ATGGAACAGT GTGCTGTGAA ACTTGCCAAA ATGGTTGGTT ATGTGAGTGC TGGGACTGTG 
GAATACCTCT ACAGCCAGGA TGGAAGCTTC TACTTTCTGG AACTGAACCC TCGGCTACAG 
GTGGAACACC CTTGTACAGA GATGGTGGCT GATGTCAACC TCCCTGCAGC ACAACTCCAG 
ATTGCCATGG GGATCCCTCT GTACAGAATC AAGGATATTC GTATGATGTA TGGGGTATCT 
CCCTGGGGTG ATGCTCCCAT TGATTTTGAA AATTCTGCTC ACGTTCCTTG CCCAAGGGGC 
CATGTTATTG CCGCTCGGAT CACTAGTGAA AATCCAGATG AGGGTTTTAA GCCCAGCTCA 
GGAACAGTTC AGGAACTGAA TTTCCGTAGC AATAAGAATG TTTGGGGTTA TTTCAGTGTT 
GCTGCTGCAG GGGGCCTTCA TGAATTTGCT GATTCTCAGT TTGGTCATTG CTTCTCCTGG 
GGAGAAAACA GAGAGGAAGC AATTTCAAAC ATGGTGGTGG CTTTGAAGGA GCTGTCTATT 
CGGGGTGACT TTCGAACCAC AGTTGAATAC CTCATCAAAT TGTTGGAGAC TGAAAGCTTT 
CAGTTGAATA GAATTGACAC CGGCTGGCTG GACAGACTGA TAGCAGAGAA AGTACAGGCA 
GAGCGACCTG ACACCATGTT GGGAGTTGTT TGTGGGGCTC TCCATGTGGC AGATGTGAGC 
CTACGGAATA GCATCTCTAA CTTCCTTCAT TCTTTAGAAA GGGGTCAAGT CCTTCCTGCT 
CATACACTTC TGAATACAGT AGATGTTGAA CTTATCTATG GGGGAGTCAA ATATGTACTT 
AAGGTGACTC GGCAGTCCCC CAACTCCTAT GTGGTGATCA TGAATGGTTC ATGCGTAGAA 
GTAGATGTGC ATCGGCTCAG TGATGGAGGG TTACTCCTGT CCTATGATGG CAGCAGTTAC 
ACCACATACA TGAAGGAAGA AGTGGATAGA TATCGTATCA CAATTGGCAA TAAGACCTGT 
GTGTTTGAGA AGGAAAATGA CCCTTCAGTG TTACGCTCAC CTTCTGCTGG GAAGTTAATC 
CAGTACATTG TGGAGGATGG AGGCCATGTG TTTGCTGGCC AGTGCTATGC TGAAATTGAG 
GTGATGAAGA TGGTAATGAC CTTAACAGCT GCAGAGTCTG GCTGTATCCA TTATGTCAAA 
CGGCCTGGAG CAGCTCTTGA CCCTGGCTGT GTAATAGCCA AAATGCAGTT GGACAACCCC 
AGCAAGGTCC AACAGGCTGA GCTTCACACA GGTAGTCTGC CACGGATCCA GAGCACTGCA 
CTCAGAGGCG AGAAGCTCCA TCGAGTGTTC CACTATGTCC TGGATAATCT GGTCAACGTA 
ATGAATGGAT ACTGCCTTCC AGATCCTTTT TTTAGCAGCA GGGTAAAAGA CTGGGTAGAG 
CGGTTGATGA AGACCCTCAG AGACCCCTCG TTACCTCTCC TAGAATTACA AGATATCATG 
ACCAGTGTCT CTGGCCGTAT TCCCCCCAAT GTTGAGAAGT CTATCAAGAA GGAAATGGCC 
CAGTATGCCA GCAATATCAC ATCAGTCCTC TGTCAGTTTC CCAGCCAGCA GATTGCCAAC 
ATCCTAGATA GCCATGCAGC TACGTTGAAC CGGAAATCTG AGCGGGAAGT CTTTTTCATG 
AACACTCAGA GCATTGTCCA GCTGGTACAG AGATACCGAA GTGGCATCCG AGGCCACATG 
AAGGCTGTGG TGATGGATCT GCTGCGGCAG TACCTGCGAG TAGAGACACA GTTCCAGAAT 
GGTCACTATG ACAAATGTGT GTTTGCCCTT CGAGAAGAGA ACAAGAGTGA TATGAACACT 
GTACTGAACT ATATCTTCTC TCATGCTCAA GTCACCAAGA AGAATCTTCT GGTCACAATG 
CTTATTGATC AGTTGTGCGG TAGGGACCCT ACTCTCACTG ATGAGCTGCT GAATATTCTC 
ACAGAGCTAA CTCAACTCAG TAAGACCACC AATGCCAAAG TAGCACTTCG AGCACGCCAG 
GTTCTTATTG CCTCCCATTT GCCATCATAT GAGCTTCGCC ATAACCAAGT AGAGTCTATC 
TTCCTTTCAG CTATTGACAT GTATGGACAT CAGTTTTGCA TTGAGAACCT GCAGAAACTC 
ATCTTGTCTG AAACATCTAT TTTTGATGTC CTACCAAACT TCTTCTATCA CAGCAACCAG 
GTAGTGAGGA TGGCAGCTCT GGAGGTTTAT GTTCGAAGGG CTTATATTGC CTATGAACTT 
AACAGCGTAC AACATCGCCA ACTTAAGGAC AACACCTGTG TGGTGGAATT CCAATTCATG 
CTACCCACAT CTCATCCAAA TAGAGGGAAC ATCCCCACGC TAAACAGAAT GTCCTTCTCC 
TCCAACCTCA ACCACTATGG CATGACTCAT GTAGCTAGTG TCAGCGATGT GCTTTTGGAC 
AACTCATTCA CTCCACCATG TCAACGGATG GGCGGAATGG TCTCTTTTCG GACTTTCGAA 
GATTTTGTCA GGATCTTTGA TGAAGTGATG GGCTGCTTCT GTGATTCTCC ACCGCAAAGC 
CCTACATTCC CTGAGGCAGG TCACACATCT CTATACGATG AAGACAAGGT CCCCAGGGAT 
GAACCAATTC ACATTCTGAA TGTGGCTATC AAGACTGACT GTGATATTGA GGATGACAGA 
CTGGCAGCTA TGTTCAGAGA GTTTACCCAA CAAAATAAAG CTACCCTGGT TGAGCATGGG 
ATACGGCGCC TTACTTTCCT GGTTGCACAA AAGAGAGAAT TTCCTAAATT TTTCACCTTC 
CGAGCAAGGG ATAAGTTTGA GGAGGATCGT ATCTATCGTC ACCTGGAACC TGCCCTCGCT 
TTCCAGTTAG AGCTGAACCG GATGAGAAAT TTTGACCTTA CTGCCATTCC ATGTGCTAAT 
CATAAAATGC ATCTGTATCT TGGGGCAGCC AAGGTGGAGG TGGGCACAGA AGTGACAGAC 
TACAGGTTCT TTGTTCGTGC AATCATCAGA CATTCTGATC TGGTCACCAA GGAAGCTTCT 
TTTGAATACC TACAAAATGA AGGGGAGAGG CTGCTTCTAG AAGCCATGGA TGAGTTGGAA 
GTTGCTTTTA ACAATACAAA CGTTCGCACT GACTGTAACC ACATCTTCCT CAACTTTGTG 
CCCACGGTCA TCATGGACCC ATCAAAGATT GAGGAATCTG TGCGGAGCAT GGTAATGCGC 
TATGGAAGTC GGCTGTGGAA ATTACGTGTC CTCCAGGCAG AACTGAAAAT CAACATTCGC 
CTGACACCAA CTGGAAAAGC AATTCCCATC CGCCTCTTCC TGACAAACGA GTCTGGCTAT 
TACTTGGACA TCAGCTTGTA TAAGGAAGTG ACTGACTCCA GGACAGCACA GATCATGTTT 
CAGGCATATG GAGACAAACA GGGACCATTG CATGGAATGT TAATCAACAC TCCATACGTC 
ACCAAAGACC TGCTTCAATC AAAGAGATTC CAGGCACAAT CCTTGGGGAC AACATACATA 
TATGATATAC CAGAGATGTT TCGGCAGTCT CTGATCAAAC TCTGGGAGTC TATGTCCACT 
CAAGCATTTC TTCCATCACC CCCCCTGCCT TCCGACATGC TGACATATAC GGAGCTCGTG 
TTGGATGATC AAGGTCAGCT GGTCCACATG AACAGGCTTC CAGGAGGAAA TGAGATTGGC 
ATGGTAGCGT GGAAAATGAC CCTTAAAAGT CCTGAATACC CAGAAGGCCG AGATATCATT 
GTTATTGGCA ATGACATCAC ATACCGAATT GGGTCCTTTG GGCCCCAGGA GGATTTGCTG 
TTTCTCAGAG CTTCTGAGCT TGCCAGGGCA GAGGGTATCC CACGCATCTT TGTAGCAGCC 
AACAGTGGAG CAAGAATTGG ACTGGCAGAG GAAATTCGTC ATATGTTTCA TGTAGCCTGG 
GTAGATCCTG AGGATCCTTA CAAGGGATAC AAATATTTAT ATCTGACCCC TCAGGATTAT 
AAGAGAGTCA GTGCTCTCAA CTCTGTCCAT TGTGAACATG TAGAGGACGA AGGAGAATCC 
AGGTACAAGA TAACTGATAT TATTGGGAAG GAAGAGGGAC TTGGAGCAGA GAACCTTCGA 
GGCTCTGGAA TGATCGCTGG AGAATCCTCA TTGGCCTATG ATGAGATCAT CACCATCAGC 
CTGGTTACAT GCCGGGCCAT TGGGATTGGA GCTTACCTTG TCCGGCTGGG ACAGAGAACC 
ATCCAGGTTG AAAATTCTCA TTTAATTTTG ACTGGAGCTG GAGCCCTCAA CAAAGTCCTT 
GGGCGGGAAG TATACACCTC CAATAACCAG CTCGGGGGCA TCCAGATCAT GCACAACAAT 
GGGGTGACCC ACAGCATTGT TTGTGATGAC TTTGAAGGGG TTTTCACTGT CCTGCACTGG 
CTATCCTACA TGCCTAAGAA TGTGCGCAGT TTAGTTCCTC TCCTGAACTC CAAGGATCCT 
ATAGATAGAA TCATCGAGTT TGTTCCCACG AAGGCCCCAT ACGATCCTCG ATGGATGCTA 
GCAGGCCGCC CTCACCCAAC TCAGAAAGGT CAGTGGCTGA GTGGATTTTT TGACTATGGC 
TCTTTCTCAG AGATCATGCA ACCCTGGGCA CAAACTGTGG TGGTTGGCAG AGCCAGGCTA 
GGAGGAATAC CTGTGGGAGT AGTTGCTGTA GAAACCCGAA CAGTGGAACT AAGTATCCCA 
GCCGATCCTG CAAACCTGGA TTCTGAAGCC AAGATAATCC AGCAGGCTGG CCAGGTTTGG 
TTCCCAGACT CTGCGTTTAA GACCTATCAG GCTATCAAGG ACTTCAACCG TGAAGGACTG 
CCTCTGATGG TCTTTGCCAA CTGGAGAGGC TTCTCTGGTG GGATGAAAGA TATGTATGAC 
CAAGTGCTGA AGTTTGGTGC CTATATTGTG GATAGCTTAC GGGAGTGCTC CCAGCCTGTG 
CTGGTATACA TTCCTCCTCA GGCTGAGCTC CGGGGTGGCT CCTGGGTTGT GATTGACCCC 
ACCATCAACC CCCGGCATAT GGAGATGTAC GCTGACCGAG AAAGCAGGGG ATCAGTTCTG 
GAGCCAGAAG GGACAGTAGA AATCAAATTC CGCAGAAAGG ATCTGGTGAA AACCATGCGT 
CGGGTGGACC CAGTCTACAT TCGCTTGGCT GAGCGATTGG GCACCCCAGA GCTCAGCGCA 
GCTGAGCGGA AGGAGCTGGA GAACAAGTTG AAGGAGCGGG AGGAATTCCT AATTCCCATT 
TACCATCAGG TAGCCGTGCA GTTTGCTGAC TTGCACGACA CACCAGGCCG GATGCAGGAG 
AAGGGTGTTA TTAATGACAT CCTGGATTGG AAAACATCCC GTACCTTTTT CTATTGGCGG 
CTGAGGCGTC TCCTGCTGGA GGACCTGGTC AAAAAGAAAA TCCACAATGC CAACCCAGAG 
CTGACCGATG GCCAGATCCA GGCCATGCTA CGGCGCTGGT TTGTAGAAGT GGAAGGAACT 
GTGAAGGCCT ACGTTTGGGA CAATAACAAG GACCTGGTGG AGTGGCTGGA GAAACAGCTG 
ACCGAGGAAG ATGGTGTTCG CTCAGTAATC GAGGAAAACA TCAAATATAT CAGCAGAGAC 
TATGTCCTCA AGCAGATCCG CAGCTTGGTA CAGGCCAATC CCGAGGTTGC CATGGACTCC 
ATCGTCCATA TGACTCAGCA TATCTCACCC ACTCAGCGAG CAGAAGTCGT ACGGATCCTC 
TCCACGATGG ACTCCCCTTC AACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624834.1
CDS	424..7530
Translation	MWWSTLMSILRVSSFWRWISAQTVRIIRALRAHFERIMDEPSPLAKPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_005624777.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624777.3

ORF Insert Sequence:

ATGTGGTGGT CCACTCTGAT GTCAATCTTG AGGGTTAGTT CTTTTTGGAG GTGGATATCT 
GCTCAGACAG TGAGAATTAT AAGAGCTTTG AGAGCTCATT TTGAAAGAAT AATGGATGAA 
CCATCCCCCT TGGCCAAACC TCTGGAGCTG AACCAGCACT CCCGATTCAT AATTGGTTCT 
GTGTCTGAAG ATAACTCAGA GGATGAAATT AGCAACCTGG TGAAGCTGGA TCTCCTGGAG 
GAGAAGGAGG GCTCTCTATC ACCAGCTTCT GTTGGCTCAG ATACACTTTC TGACTTGGGG 
ATCTCCAGCC TACAGGACGG CTTGGCCTTA CACATGAGGT CCAGCATGTC TGGCTTGCAT 
CTAGTGAAGC AAGGTCGAGA CCGAAAGAAA ATAGACTCTC AGCGAGATTT CACTGTAGCT 
TCTCCAGCAG AATTTGTTAC TCGTTTTGGG GGAAATAAAG TGATTGAGAA GGTTCTTATT 
GCCAACAATG GCATTGCAGC AGTGAAATGC ATGCGGTCTA TCCGTCGATG GTCTTATGAG 
ATGTTTCGAA ATGAACGTGC AATCCGATTT GTTGTTATGG TAACACCTGA AGACCTGAAA 
GCCAATGCAG AATACATTAA GATGGCGGAT CACTATGTGC CAGTGCCAGG AGGACCAAAT 
AACAACAACT ATGCGAACGT GGAATTAATT CTTGATATTG CTAAAAGGAT TCCCGTACAA 
GCAGTGTGGG CTGGCTGGGG TCATGCTTCT GAGAATCCCA AGCTCCCAGA ACTTCTTTTG 
AAAAATGGCA TCGCCTTCAT GGGCCCTCCA AGCCAGGCCA TGTGGGCTTT GGGGGATAAG 
ATTGCATCTT CTATAGTGGC TCAAACTGCA GGTATCCCAA CTCTTCCCTG GAGTGGCAGT 
GGTCTTCGGG TGGACTGGCA TGAAAATGAT TTTTCCAAAC GTATCTTAAA TGTTCCCCAG 
GAATTATACG AAAAAGGTTA TGTGAAGGAT GTGGATGATG GGCTAAAGGC AGCGGAGGAA 
GTTGGATATC CAGTAATGAT CAAGGCCTCA GAAGGAGGAG GAGGGAAAGG AATCAGAAAA 
GTCAACAATG CAGATGATTT CCCTAACCTC TTCAGACAGG TTCAAGCTGA AGTCCCTGGA 
TCTCCTATAT TTGTGATGAG ACTAGCCAAA CAATCTCGTC ATCTGGAGGT ACAGATCTTA 
GCAGATCAAT ATGGCAATGC TATCTCTCTC TTTGGTCGTG ACTGCTCTGT ACAGCGCAGG 
CATCAGAAGA TTATTGAGGA AGCACCTGCT GCTATTGCTA CTCCAGCAGT ATTTGAACAT 
ATGGAACAGT GTGCTGTGAA ACTTGCCAAA ATGGTTGGTT ATGTGAGTGC TGGGACTGTG 
GAATACCTCT ACAGCCAGGA TGGAAGCTTC TACTTTCTGG AACTGAACCC TCGGCTACAG 
GTGGAACACC CTTGTACAGA GATGGTGGCT GATGTCAACC TCCCTGCAGC ACAACTCCAG 
ATTGCCATGG GGATCCCTCT GTACAGAATC AAGGATATTC GTATGATGTA TGGGGTATCT 
CCCTGGGGTG ATGCTCCCAT TGATTTTGAA AATTCTGCTC ACGTTCCTTG CCCAAGGGGC 
CATGTTATTG CCGCTCGGAT CACTAGTGAA AATCCAGATG AGGGTTTTAA GCCCAGCTCA 
GGAACAGTTC AGGAACTGAA TTTCCGTAGC AATAAGAATG TTTGGGGTTA TTTCAGTGTT 
GCTGCTGCAG GGGGCCTTCA TGAATTTGCT GATTCTCAGT TTGGTCATTG CTTCTCCTGG 
GGAGAAAACA GAGAGGAAGC AATTTCAAAC ATGGTGGTGG CTTTGAAGGA GCTGTCTATT 
CGGGGTGACT TTCGAACCAC AGTTGAATAC CTCATCAAAT TGTTGGAGAC TGAAAGCTTT 
CAGTTGAATA GAATTGACAC CGGCTGGCTG GACAGACTGA TAGCAGAGAA AGTACAGGCA 
GAGCGACCTG ACACCATGTT GGGAGTTGTT TGTGGGGCTC TCCATGTGGC AGATGTGAGC 
CTACGGAATA GCATCTCTAA CTTCCTTCAT TCTTTAGAAA GGGGTCAAGT CCTTCCTGCT 
CATACACTTC TGAATACAGT AGATGTTGAA CTTATCTATG GGGGAGTCAA ATATGTACTT 
AAGGTGACTC GGCAGTCCCC CAACTCCTAT GTGGTGATCA TGAATGGTTC ATGCGTAGAA 
GTAGATGTGC ATCGGCTCAG TGATGGAGGG TTACTCCTGT CCTATGATGG CAGCAGTTAC 
ACCACATACA TGAAGGAAGA AGTGGATAGA TATCGTATCA CAATTGGCAA TAAGACCTGT 
GTGTTTGAGA AGGAAAATGA CCCTTCAGTG TTACGCTCAC CTTCTGCTGG GAAGTTAATC 
CAGTACATTG TGGAGGATGG AGGCCATGTG TTTGCTGGCC AGTGCTATGC TGAAATTGAG 
GTGATGAAGA TGGTAATGAC CTTAACAGCT GCAGAGTCTG GCTGTATCCA TTATGTCAAA 
CGGCCTGGAG CAGCTCTTGA CCCTGGCTGT GTAATAGCCA AAATGCAGTT GGACAACCCC 
AGCAAGGTCC AACAGGCTGA GCTTCACACA GGTAGTCTGC CACGGATCCA GAGCACTGCA 
CTCAGAGGCG AGAAGCTCCA TCGAGTGTTC CACTATGTCC TGGATAATCT GGTCAACGTA 
ATGAATGGAT ACTGCCTTCC AGATCCTTTT TTTAGCAGCA GGGTAAAAGA CTGGGTAGAG 
CGGTTGATGA AGACCCTCAG AGACCCCTCG TTACCTCTCC TAGAATTACA AGATATCATG 
ACCAGTGTCT CTGGCCGTAT TCCCCCCAAT GTTGAGAAGT CTATCAAGAA GGAAATGGCC 
CAGTATGCCA GCAATATCAC ATCAGTCCTC TGTCAGTTTC CCAGCCAGCA GATTGCCAAC 
ATCCTAGATA GCCATGCAGC TACGTTGAAC CGGAAATCTG AGCGGGAAGT CTTTTTCATG 
AACACTCAGA GCATTGTCCA GCTGGTACAG AGATACCGAA GTGGCATCCG AGGCCACATG 
AAGGCTGTGG TGATGGATCT GCTGCGGCAG TACCTGCGAG TAGAGACACA GTTCCAGAAT 
GGTCACTATG ACAAATGTGT GTTTGCCCTT CGAGAAGAGA ACAAGAGTGA TATGAACACT 
GTACTGAACT ATATCTTCTC TCATGCTCAA GTCACCAAGA AGAATCTTCT GGTCACAATG 
CTTATTGATC AGTTGTGCGG TAGGGACCCT ACTCTCACTG ATGAGCTGCT GAATATTCTC 
ACAGAGCTAA CTCAACTCAG TAAGACCACC AATGCCAAAG TAGCACTTCG AGCACGCCAG 
GTTCTTATTG CCTCCCATTT GCCATCATAT GAGCTTCGCC ATAACCAAGT AGAGTCTATC 
TTCCTTTCAG CTATTGACAT GTATGGACAT CAGTTTTGCA TTGAGAACCT GCAGAAACTC 
ATCTTGTCTG AAACATCTAT TTTTGATGTC CTACCAAACT TCTTCTATCA CAGCAACCAG 
GTAGTGAGGA TGGCAGCTCT GGAGGTTTAT GTTCGAAGGG CTTATATTGC CTATGAACTT 
AACAGCGTAC AACATCGCCA ACTTAAGGAC AACACCTGTG TGGTGGAATT CCAATTCATG 
CTACCCACAT CTCATCCAAA TAGAGGGAAC ATCCCCACGC TAAACAGAAT GTCCTTCTCC 
TCCAACCTCA ACCACTATGG CATGACTCAT GTAGCTAGTG TCAGCGATGT GCTTTTGGAC 
AACTCATTCA CTCCACCATG TCAACGGATG GGCGGAATGG TCTCTTTTCG GACTTTCGAA 
GATTTTGTCA GGATCTTTGA TGAAGTGATG GGCTGCTTCT GTGATTCTCC ACCGCAAAGC 
CCTACATTCC CTGAGGCAGG TCACACATCT CTATACGATG AAGACAAGGT CCCCAGGGAT 
GAACCAATTC ACATTCTGAA TGTGGCTATC AAGACTGACT GTGATATTGA GGATGACAGA 
CTGGCAGCTA TGTTCAGAGA GTTTACCCAA CAAAATAAAG CTACCCTGGT TGAGCATGGG 
ATACGGCGCC TTACTTTCCT GGTTGCACAA AAGAGAGAAT TTCCTAAATT TTTCACCTTC 
CGAGCAAGGG ATAAGTTTGA GGAGGATCGT ATCTATCGTC ACCTGGAACC TGCCCTCGCT 
TTCCAGTTAG AGCTGAACCG GATGAGAAAT TTTGACCTTA CTGCCATTCC ATGTGCTAAT 
CATAAAATGC ATCTGTATCT TGGGGCAGCC AAGGTGGAGG TGGGCACAGA AGTGACAGAC 
TACAGGTTCT TTGTTCGTGC AATCATCAGA CATTCTGATC TGGTCACCAA GGAAGCTTCT 
TTTGAATACC TACAAAATGA AGGGGAGAGG CTGCTTCTAG AAGCCATGGA TGAGTTGGAA 
GTTGCTTTTA ACAATACAAA CGTTCGCACT GACTGTAACC ACATCTTCCT CAACTTTGTG 
CCCACGGTCA TCATGGACCC ATCAAAGATT GAGGAATCTG TGCGGAGCAT GGTAATGCGC 
TATGGAAGTC GGCTGTGGAA ATTACGTGTC CTCCAGGCAG AACTGAAAAT CAACATTCGC 
CTGACACCAA CTGGAAAAGC AATTCCCATC CGCCTCTTCC TGACAAACGA GTCTGGCTAT 
TACTTGGACA TCAGCTTGTA TAAGGAAGTG ACTGACTCCA GGACAGCACA GATCATGTTT 
CAGGCATATG GAGACAAACA GGGACCATTG CATGGAATGT TAATCAACAC TCCATACGTC 
ACCAAAGACC TGCTTCAATC AAAGAGATTC CAGGCACAAT CCTTGGGGAC AACATACATA 
TATGATATAC CAGAGATGTT TCGGCAGTCT CTGATCAAAC TCTGGGAGTC TATGTCCACT 
CAAGCATTTC TTCCATCACC CCCCCTGCCT TCCGACATGC TGACATATAC GGAGCTCGTG 
TTGGATGATC AAGGTCAGCT GGTCCACATG AACAGGCTTC CAGGAGGAAA TGAGATTGGC 
ATGGTAGCGT GGAAAATGAC CCTTAAAAGT CCTGAATACC CAGAAGGCCG AGATATCATT 
GTTATTGGCA ATGACATCAC ATACCGAATT GGGTCCTTTG GGCCCCAGGA GGATTTGCTG 
TTTCTCAGAG CTTCTGAGCT TGCCAGGGCA GAGGGTATCC CACGCATCTT TGTAGCAGCC 
AACAGTGGAG CAAGAATTGG ACTGGCAGAG GAAATTCGTC ATATGTTTCA TGTAGCCTGG 
GTAGATCCTG AGGATCCTTA CAAGGGATAC AAATATTTAT ATCTGACCCC TCAGGATTAT 
AAGAGAGTCA GTGCTCTCAA CTCTGTCCAT TGTGAACATG TAGAGGACGA AGGAGAATCC 
AGGTACAAGA TAACTGATAT TATTGGGAAG GAAGAGGGAC TTGGAGCAGA GAACCTTCGA 
GGCTCTGGAA TGATCGCTGG AGAATCCTCA TTGGCCTATG ATGAGATCAT CACCATCAGC 
CTGGTTACAT GCCGGGCCAT TGGGATTGGA GCTTACCTTG TCCGGCTGGG ACAGAGAACC 
ATCCAGGTTG AAAATTCTCA TTTAATTTTG ACTGGAGCTG GAGCCCTCAA CAAAGTCCTT 
GGGCGGGAAG TATACACCTC CAATAACCAG CTCGGGGGCA TCCAGATCAT GCACAACAAT 
GGGGTGACCC ACAGCATTGT TTGTGATGAC TTTGAAGGGG TTTTCACTGT CCTGCACTGG 
CTATCCTACA TGCCTAAGAA TGTGCGCAGT TTAGTTCCTC TCCTGAACTC CAAGGATCCT 
ATAGATAGAA TCATCGAGTT TGTTCCCACG AAGGCCCCAT ACGATCCTCG ATGGATGCTA 
GCAGGCCGCC CTCACCCAAC TCAGAAAGGT CAGTGGCTGA GTGGATTTTT TGACTATGGC 
TCTTTCTCAG AGATCATGCA ACCCTGGGCA CAAACTGTGG TGGTTGGCAG AGCCAGGCTA 
GGAGGAATAC CTGTGGGAGT AGTTGCTGTA GAAACCCGAA CAGTGGAACT AAGTATCCCA 
GCCGATCCTG CAAACCTGGA TTCTGAAGCC AAGATAATCC AGCAGGCTGG CCAGGTTTGG 
TTCCCAGACT CTGCGTTTAA GACCTATCAG GCTATCAAGG ACTTCAACCG TGAAGGACTG 
CCTCTGATGG TCTTTGCCAA CTGGAGAGGC TTCTCTGGTG GGATGAAAGA TATGTATGAC 
CAAGTGCTGA AGTTTGGTGC CTATATTGTG GATAGCTTAC GGGAGTGCTC CCAGCCTGTG 
CTGGTATACA TTCCTCCTCA GGCTGAGCTC CGGGGTGGCT CCTGGGTTGT GATTGACCCC 
ACCATCAACC CCCGGCATAT GGAGATGTAC GCTGACCGAG AAAGCAGGGG ATCAGTTCTG 
GAGCCAGAAG GGACAGTAGA AATCAAATTC CGCAGAAAGG ATCTGGTGAA AACCATGCGT 
CGGGTGGACC CAGTCTACAT TCGCTTGGCT GAGCGATTGG GCACCCCAGA GCTCAGCGCA 
GCTGAGCGGA AGGAGCTGGA GAACAAGTTG AAGGAGCGGG AGGAATTCCT AATTCCCATT 
TACCATCAGG TAGCCGTGCA GTTTGCTGAC TTGCACGACA CACCAGGCCG GATGCAGGAG 
AAGGGTGTTA TTAATGACAT CCTGGATTGG AAAACATCCC GTACCTTTTT CTATTGGCGG 
CTGAGGCGTC TCCTGCTGGA GGACCTGGTC AAAAAGAAAA TCCACAATGC CAACCCAGAG 
CTGACCGATG GCCAGATCCA GGCCATGCTA CGGCGCTGGT TTGTAGAAGT GGAAGGAACT 
GTGAAGGCCT ACGTTTGGGA CAATAACAAG GACCTGGTGG AGTGGCTGGA GAAACAGCTG 
ACCGAGGAAG ATGGTGTTCG CTCAGTAATC GAGGAAAACA TCAAATATAT CAGCAGAGAC 
TATGTCCTCA AGCAGATCCG CAGCTTGGTA CAGGCCAATC CCGAGGTTGC CATGGACTCC 
ATCGTCCATA TGACTCAGCA TATCTCACCC ACTCAGCGAG CAGAAGTCGT ACGGATCCTC 
TCCACGATGG ACTCCCCTTC AACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_005624777.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624834.1 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X3, mRNA.

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CloneID	OCe12092
Clone ID Related Accession (Same CDS sequence)	XM_005624777.3 , XM_005624777.2
Accession Version	XM_005624777.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	acetyl-CoA carboxylase 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTGGT CCACTCTGAT GTCAATCTTG AGGGTTAGTT CTTTTTGGAG GTGGATATCT 
GCTCAGACAG TGAGAATTAT AAGAGCTTTG AGAGCTCATT TTGAAAGAAT AATGGATGAA 
CCATCCCCCT TGGCCAAACC TCTGGAGCTG AACCAGCACT CCCGATTCAT AATTGGTTCT 
GTGTCTGAAG ATAACTCAGA GGATGAAATT AGCAACCTGG TGAAGCTGGA TCTCCTGGAG 
GAGAAGGAGG GCTCTCTATC ACCAGCTTCT GTTGGCTCAG ATACACTTTC TGACTTGGGG 
ATCTCCAGCC TACAGGACGG CTTGGCCTTA CACATGAGGT CCAGCATGTC TGGCTTGCAT 
CTAGTGAAGC AAGGTCGAGA CCGAAAGAAA ATAGACTCTC AGCGAGATTT CACTGTAGCT 
TCTCCAGCAG AATTTGTTAC TCGTTTTGGG GGAAATAAAG TGATTGAGAA GGTTCTTATT 
GCCAACAATG GCATTGCAGC AGTGAAATGC ATGCGGTCTA TCCGTCGATG GTCTTATGAG 
ATGTTTCGAA ATGAACGTGC AATCCGATTT GTTGTTATGG TAACACCTGA AGACCTGAAA 
GCCAATGCAG AATACATTAA GATGGCGGAT CACTATGTGC CAGTGCCAGG AGGACCAAAT 
AACAACAACT ATGCGAACGT GGAATTAATT CTTGATATTG CTAAAAGGAT TCCCGTACAA 
GCAGTGTGGG CTGGCTGGGG TCATGCTTCT GAGAATCCCA AGCTCCCAGA ACTTCTTTTG 
AAAAATGGCA TCGCCTTCAT GGGCCCTCCA AGCCAGGCCA TGTGGGCTTT GGGGGATAAG 
ATTGCATCTT CTATAGTGGC TCAAACTGCA GGTATCCCAA CTCTTCCCTG GAGTGGCAGT 
GGTCTTCGGG TGGACTGGCA TGAAAATGAT TTTTCCAAAC GTATCTTAAA TGTTCCCCAG 
GAATTATACG AAAAAGGTTA TGTGAAGGAT GTGGATGATG GGCTAAAGGC AGCGGAGGAA 
GTTGGATATC CAGTAATGAT CAAGGCCTCA GAAGGAGGAG GAGGGAAAGG AATCAGAAAA 
GTCAACAATG CAGATGATTT CCCTAACCTC TTCAGACAGG TTCAAGCTGA AGTCCCTGGA 
TCTCCTATAT TTGTGATGAG ACTAGCCAAA CAATCTCGTC ATCTGGAGGT ACAGATCTTA 
GCAGATCAAT ATGGCAATGC TATCTCTCTC TTTGGTCGTG ACTGCTCTGT ACAGCGCAGG 
CATCAGAAGA TTATTGAGGA AGCACCTGCT GCTATTGCTA CTCCAGCAGT ATTTGAACAT 
ATGGAACAGT GTGCTGTGAA ACTTGCCAAA ATGGTTGGTT ATGTGAGTGC TGGGACTGTG 
GAATACCTCT ACAGCCAGGA TGGAAGCTTC TACTTTCTGG AACTGAACCC TCGGCTACAG 
GTGGAACACC CTTGTACAGA GATGGTGGCT GATGTCAACC TCCCTGCAGC ACAACTCCAG 
ATTGCCATGG GGATCCCTCT GTACAGAATC AAGGATATTC GTATGATGTA TGGGGTATCT 
CCCTGGGGTG ATGCTCCCAT TGATTTTGAA AATTCTGCTC ACGTTCCTTG CCCAAGGGGC 
CATGTTATTG CCGCTCGGAT CACTAGTGAA AATCCAGATG AGGGTTTTAA GCCCAGCTCA 
GGAACAGTTC AGGAACTGAA TTTCCGTAGC AATAAGAATG TTTGGGGTTA TTTCAGTGTT 
GCTGCTGCAG GGGGCCTTCA TGAATTTGCT GATTCTCAGT TTGGTCATTG CTTCTCCTGG 
GGAGAAAACA GAGAGGAAGC AATTTCAAAC ATGGTGGTGG CTTTGAAGGA GCTGTCTATT 
CGGGGTGACT TTCGAACCAC AGTTGAATAC CTCATCAAAT TGTTGGAGAC TGAAAGCTTT 
CAGTTGAATA GAATTGACAC CGGCTGGCTG GACAGACTGA TAGCAGAGAA AGTACAGGCA 
GAGCGACCTG ACACCATGTT GGGAGTTGTT TGTGGGGCTC TCCATGTGGC AGATGTGAGC 
CTACGGAATA GCATCTCTAA CTTCCTTCAT TCTTTAGAAA GGGGTCAAGT CCTTCCTGCT 
CATACACTTC TGAATACAGT AGATGTTGAA CTTATCTATG GGGGAGTCAA ATATGTACTT 
AAGGTGACTC GGCAGTCCCC CAACTCCTAT GTGGTGATCA TGAATGGTTC ATGCGTAGAA 
GTAGATGTGC ATCGGCTCAG TGATGGAGGG TTACTCCTGT CCTATGATGG CAGCAGTTAC 
ACCACATACA TGAAGGAAGA AGTGGATAGA TATCGTATCA CAATTGGCAA TAAGACCTGT 
GTGTTTGAGA AGGAAAATGA CCCTTCAGTG TTACGCTCAC CTTCTGCTGG GAAGTTAATC 
CAGTACATTG TGGAGGATGG AGGCCATGTG TTTGCTGGCC AGTGCTATGC TGAAATTGAG 
GTGATGAAGA TGGTAATGAC CTTAACAGCT GCAGAGTCTG GCTGTATCCA TTATGTCAAA 
CGGCCTGGAG CAGCTCTTGA CCCTGGCTGT GTAATAGCCA AAATGCAGTT GGACAACCCC 
AGCAAGGTCC AACAGGCTGA GCTTCACACA GGTAGTCTGC CACGGATCCA GAGCACTGCA 
CTCAGAGGCG AGAAGCTCCA TCGAGTGTTC CACTATGTCC TGGATAATCT GGTCAACGTA 
ATGAATGGAT ACTGCCTTCC AGATCCTTTT TTTAGCAGCA GGGTAAAAGA CTGGGTAGAG 
CGGTTGATGA AGACCCTCAG AGACCCCTCG TTACCTCTCC TAGAATTACA AGATATCATG 
ACCAGTGTCT CTGGCCGTAT TCCCCCCAAT GTTGAGAAGT CTATCAAGAA GGAAATGGCC 
CAGTATGCCA GCAATATCAC ATCAGTCCTC TGTCAGTTTC CCAGCCAGCA GATTGCCAAC 
ATCCTAGATA GCCATGCAGC TACGTTGAAC CGGAAATCTG AGCGGGAAGT CTTTTTCATG 
AACACTCAGA GCATTGTCCA GCTGGTACAG AGATACCGAA GTGGCATCCG AGGCCACATG 
AAGGCTGTGG TGATGGATCT GCTGCGGCAG TACCTGCGAG TAGAGACACA GTTCCAGAAT 
GGTCACTATG ACAAATGTGT GTTTGCCCTT CGAGAAGAGA ACAAGAGTGA TATGAACACT 
GTACTGAACT ATATCTTCTC TCATGCTCAA GTCACCAAGA AGAATCTTCT GGTCACAATG 
CTTATTGATC AGTTGTGCGG TAGGGACCCT ACTCTCACTG ATGAGCTGCT GAATATTCTC 
ACAGAGCTAA CTCAACTCAG TAAGACCACC AATGCCAAAG TAGCACTTCG AGCACGCCAG 
GTTCTTATTG CCTCCCATTT GCCATCATAT GAGCTTCGCC ATAACCAAGT AGAGTCTATC 
TTCCTTTCAG CTATTGACAT GTATGGACAT CAGTTTTGCA TTGAGAACCT GCAGAAACTC 
ATCTTGTCTG AAACATCTAT TTTTGATGTC CTACCAAACT TCTTCTATCA CAGCAACCAG 
GTAGTGAGGA TGGCAGCTCT GGAGGTTTAT GTTCGAAGGG CTTATATTGC CTATGAACTT 
AACAGCGTAC AACATCGCCA ACTTAAGGAC AACACCTGTG TGGTGGAATT CCAATTCATG 
CTACCCACAT CTCATCCAAA TAGAGGGAAC ATCCCCACGC TAAACAGAAT GTCCTTCTCC 
TCCAACCTCA ACCACTATGG CATGACTCAT GTAGCTAGTG TCAGCGATGT GCTTTTGGAC 
AACTCATTCA CTCCACCATG TCAACGGATG GGCGGAATGG TCTCTTTTCG GACTTTCGAA 
GATTTTGTCA GGATCTTTGA TGAAGTGATG GGCTGCTTCT GTGATTCTCC ACCGCAAAGC 
CCTACATTCC CTGAGGCAGG TCACACATCT CTATACGATG AAGACAAGGT CCCCAGGGAT 
GAACCAATTC ACATTCTGAA TGTGGCTATC AAGACTGACT GTGATATTGA GGATGACAGA 
CTGGCAGCTA TGTTCAGAGA GTTTACCCAA CAAAATAAAG CTACCCTGGT TGAGCATGGG 
ATACGGCGCC TTACTTTCCT GGTTGCACAA AAGAGAGAAT TTCCTAAATT TTTCACCTTC 
CGAGCAAGGG ATAAGTTTGA GGAGGATCGT ATCTATCGTC ACCTGGAACC TGCCCTCGCT 
TTCCAGTTAG AGCTGAACCG GATGAGAAAT TTTGACCTTA CTGCCATTCC ATGTGCTAAT 
CATAAAATGC ATCTGTATCT TGGGGCAGCC AAGGTGGAGG TGGGCACAGA AGTGACAGAC 
TACAGGTTCT TTGTTCGTGC AATCATCAGA CATTCTGATC TGGTCACCAA GGAAGCTTCT 
TTTGAATACC TACAAAATGA AGGGGAGAGG CTGCTTCTAG AAGCCATGGA TGAGTTGGAA 
GTTGCTTTTA ACAATACAAA CGTTCGCACT GACTGTAACC ACATCTTCCT CAACTTTGTG 
CCCACGGTCA TCATGGACCC ATCAAAGATT GAGGAATCTG TGCGGAGCAT GGTAATGCGC 
TATGGAAGTC GGCTGTGGAA ATTACGTGTC CTCCAGGCAG AACTGAAAAT CAACATTCGC 
CTGACACCAA CTGGAAAAGC AATTCCCATC CGCCTCTTCC TGACAAACGA GTCTGGCTAT 
TACTTGGACA TCAGCTTGTA TAAGGAAGTG ACTGACTCCA GGACAGCACA GATCATGTTT 
CAGGCATATG GAGACAAACA GGGACCATTG CATGGAATGT TAATCAACAC TCCATACGTC 
ACCAAAGACC TGCTTCAATC AAAGAGATTC CAGGCACAAT CCTTGGGGAC AACATACATA 
TATGATATAC CAGAGATGTT TCGGCAGTCT CTGATCAAAC TCTGGGAGTC TATGTCCACT 
CAAGCATTTC TTCCATCACC CCCCCTGCCT TCCGACATGC TGACATATAC GGAGCTCGTG 
TTGGATGATC AAGGTCAGCT GGTCCACATG AACAGGCTTC CAGGAGGAAA TGAGATTGGC 
ATGGTAGCGT GGAAAATGAC CCTTAAAAGT CCTGAATACC CAGAAGGCCG AGATATCATT 
GTTATTGGCA ATGACATCAC ATACCGAATT GGGTCCTTTG GGCCCCAGGA GGATTTGCTG 
TTTCTCAGAG CTTCTGAGCT TGCCAGGGCA GAGGGTATCC CACGCATCTT TGTAGCAGCC 
AACAGTGGAG CAAGAATTGG ACTGGCAGAG GAAATTCGTC ATATGTTTCA TGTAGCCTGG 
GTAGATCCTG AGGATCCTTA CAAGGGATAC AAATATTTAT ATCTGACCCC TCAGGATTAT 
AAGAGAGTCA GTGCTCTCAA CTCTGTCCAT TGTGAACATG TAGAGGACGA AGGAGAATCC 
AGGTACAAGA TAACTGATAT TATTGGGAAG GAAGAGGGAC TTGGAGCAGA GAACCTTCGA 
GGCTCTGGAA TGATCGCTGG AGAATCCTCA TTGGCCTATG ATGAGATCAT CACCATCAGC 
CTGGTTACAT GCCGGGCCAT TGGGATTGGA GCTTACCTTG TCCGGCTGGG ACAGAGAACC 
ATCCAGGTTG AAAATTCTCA TTTAATTTTG ACTGGAGCTG GAGCCCTCAA CAAAGTCCTT 
GGGCGGGAAG TATACACCTC CAATAACCAG CTCGGGGGCA TCCAGATCAT GCACAACAAT 
GGGGTGACCC ACAGCATTGT TTGTGATGAC TTTGAAGGGG TTTTCACTGT CCTGCACTGG 
CTATCCTACA TGCCTAAGAA TGTGCGCAGT TTAGTTCCTC TCCTGAACTC CAAGGATCCT 
ATAGATAGAA TCATCGAGTT TGTTCCCACG AAGGCCCCAT ACGATCCTCG ATGGATGCTA 
GCAGGCCGCC CTCACCCAAC TCAGAAAGGT CAGTGGCTGA GTGGATTTTT TGACTATGGC 
TCTTTCTCAG AGATCATGCA ACCCTGGGCA CAAACTGTGG TGGTTGGCAG AGCCAGGCTA 
GGAGGAATAC CTGTGGGAGT AGTTGCTGTA GAAACCCGAA CAGTGGAACT AAGTATCCCA 
GCCGATCCTG CAAACCTGGA TTCTGAAGCC AAGATAATCC AGCAGGCTGG CCAGGTTTGG 
TTCCCAGACT CTGCGTTTAA GACCTATCAG GCTATCAAGG ACTTCAACCG TGAAGGACTG 
CCTCTGATGG TCTTTGCCAA CTGGAGAGGC TTCTCTGGTG GGATGAAAGA TATGTATGAC 
CAAGTGCTGA AGTTTGGTGC CTATATTGTG GATAGCTTAC GGGAGTGCTC CCAGCCTGTG 
CTGGTATACA TTCCTCCTCA GGCTGAGCTC CGGGGTGGCT CCTGGGTTGT GATTGACCCC 
ACCATCAACC CCCGGCATAT GGAGATGTAC GCTGACCGAG AAAGCAGGGG ATCAGTTCTG 
GAGCCAGAAG GGACAGTAGA AATCAAATTC CGCAGAAAGG ATCTGGTGAA AACCATGCGT 
CGGGTGGACC CAGTCTACAT TCGCTTGGCT GAGCGATTGG GCACCCCAGA GCTCAGCGCA 
GCTGAGCGGA AGGAGCTGGA GAACAAGTTG AAGGAGCGGG AGGAATTCCT AATTCCCATT 
TACCATCAGG TAGCCGTGCA GTTTGCTGAC TTGCACGACA CACCAGGCCG GATGCAGGAG 
AAGGGTGTTA TTAATGACAT CCTGGATTGG AAAACATCCC GTACCTTTTT CTATTGGCGG 
CTGAGGCGTC TCCTGCTGGA GGACCTGGTC AAAAAGAAAA TCCACAATGC CAACCCAGAG 
CTGACCGATG GCCAGATCCA GGCCATGCTA CGGCGCTGGT TTGTAGAAGT GGAAGGAACT 
GTGAAGGCCT ACGTTTGGGA CAATAACAAG GACCTGGTGG AGTGGCTGGA GAAACAGCTG 
ACCGAGGAAG ATGGTGTTCG CTCAGTAATC GAGGAAAACA TCAAATATAT CAGCAGAGAC 
TATGTCCTCA AGCAGATCCG CAGCTTGGTA CAGGCCAATC CCGAGGTTGC CATGGACTCC 
ATCGTCCATA TGACTCAGCA TATCTCACCC ACTCAGCGAG CAGAAGTCGT ACGGATCCTC 
TCCACGATGG ACTCCCCTTC AACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624834.1
CDS	434..7540
Translation	MWWSTLMSILRVSSFWRWISAQTVRIIRALRAHFERIMDEPSPLAKPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_005624777.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624777.2

ORF Insert Sequence:

ATGTGGTGGT CCACTCTGAT GTCAATCTTG AGGGTTAGTT CTTTTTGGAG GTGGATATCT 
GCTCAGACAG TGAGAATTAT AAGAGCTTTG AGAGCTCATT TTGAAAGAAT AATGGATGAA 
CCATCCCCCT TGGCCAAACC TCTGGAGCTG AACCAGCACT CCCGATTCAT AATTGGTTCT 
GTGTCTGAAG ATAACTCAGA GGATGAAATT AGCAACCTGG TGAAGCTGGA TCTCCTGGAG 
GAGAAGGAGG GCTCTCTATC ACCAGCTTCT GTTGGCTCAG ATACACTTTC TGACTTGGGG 
ATCTCCAGCC TACAGGACGG CTTGGCCTTA CACATGAGGT CCAGCATGTC TGGCTTGCAT 
CTAGTGAAGC AAGGTCGAGA CCGAAAGAAA ATAGACTCTC AGCGAGATTT CACTGTAGCT 
TCTCCAGCAG AATTTGTTAC TCGTTTTGGG GGAAATAAAG TGATTGAGAA GGTTCTTATT 
GCCAACAATG GCATTGCAGC AGTGAAATGC ATGCGGTCTA TCCGTCGATG GTCTTATGAG 
ATGTTTCGAA ATGAACGTGC AATCCGATTT GTTGTTATGG TAACACCTGA AGACCTGAAA 
GCCAATGCAG AATACATTAA GATGGCGGAT CACTATGTGC CAGTGCCAGG AGGACCAAAT 
AACAACAACT ATGCGAACGT GGAATTAATT CTTGATATTG CTAAAAGGAT TCCCGTACAA 
GCAGTGTGGG CTGGCTGGGG TCATGCTTCT GAGAATCCCA AGCTCCCAGA ACTTCTTTTG 
AAAAATGGCA TCGCCTTCAT GGGCCCTCCA AGCCAGGCCA TGTGGGCTTT GGGGGATAAG 
ATTGCATCTT CTATAGTGGC TCAAACTGCA GGTATCCCAA CTCTTCCCTG GAGTGGCAGT 
GGTCTTCGGG TGGACTGGCA TGAAAATGAT TTTTCCAAAC GTATCTTAAA TGTTCCCCAG 
GAATTATACG AAAAAGGTTA TGTGAAGGAT GTGGATGATG GGCTAAAGGC AGCGGAGGAA 
GTTGGATATC CAGTAATGAT CAAGGCCTCA GAAGGAGGAG GAGGGAAAGG AATCAGAAAA 
GTCAACAATG CAGATGATTT CCCTAACCTC TTCAGACAGG TTCAAGCTGA AGTCCCTGGA 
TCTCCTATAT TTGTGATGAG ACTAGCCAAA CAATCTCGTC ATCTGGAGGT ACAGATCTTA 
GCAGATCAAT ATGGCAATGC TATCTCTCTC TTTGGTCGTG ACTGCTCTGT ACAGCGCAGG 
CATCAGAAGA TTATTGAGGA AGCACCTGCT GCTATTGCTA CTCCAGCAGT ATTTGAACAT 
ATGGAACAGT GTGCTGTGAA ACTTGCCAAA ATGGTTGGTT ATGTGAGTGC TGGGACTGTG 
GAATACCTCT ACAGCCAGGA TGGAAGCTTC TACTTTCTGG AACTGAACCC TCGGCTACAG 
GTGGAACACC CTTGTACAGA GATGGTGGCT GATGTCAACC TCCCTGCAGC ACAACTCCAG 
ATTGCCATGG GGATCCCTCT GTACAGAATC AAGGATATTC GTATGATGTA TGGGGTATCT 
CCCTGGGGTG ATGCTCCCAT TGATTTTGAA AATTCTGCTC ACGTTCCTTG CCCAAGGGGC 
CATGTTATTG CCGCTCGGAT CACTAGTGAA AATCCAGATG AGGGTTTTAA GCCCAGCTCA 
GGAACAGTTC AGGAACTGAA TTTCCGTAGC AATAAGAATG TTTGGGGTTA TTTCAGTGTT 
GCTGCTGCAG GGGGCCTTCA TGAATTTGCT GATTCTCAGT TTGGTCATTG CTTCTCCTGG 
GGAGAAAACA GAGAGGAAGC AATTTCAAAC ATGGTGGTGG CTTTGAAGGA GCTGTCTATT 
CGGGGTGACT TTCGAACCAC AGTTGAATAC CTCATCAAAT TGTTGGAGAC TGAAAGCTTT 
CAGTTGAATA GAATTGACAC CGGCTGGCTG GACAGACTGA TAGCAGAGAA AGTACAGGCA 
GAGCGACCTG ACACCATGTT GGGAGTTGTT TGTGGGGCTC TCCATGTGGC AGATGTGAGC 
CTACGGAATA GCATCTCTAA CTTCCTTCAT TCTTTAGAAA GGGGTCAAGT CCTTCCTGCT 
CATACACTTC TGAATACAGT AGATGTTGAA CTTATCTATG GGGGAGTCAA ATATGTACTT 
AAGGTGACTC GGCAGTCCCC CAACTCCTAT GTGGTGATCA TGAATGGTTC ATGCGTAGAA 
GTAGATGTGC ATCGGCTCAG TGATGGAGGG TTACTCCTGT CCTATGATGG CAGCAGTTAC 
ACCACATACA TGAAGGAAGA AGTGGATAGA TATCGTATCA CAATTGGCAA TAAGACCTGT 
GTGTTTGAGA AGGAAAATGA CCCTTCAGTG TTACGCTCAC CTTCTGCTGG GAAGTTAATC 
CAGTACATTG TGGAGGATGG AGGCCATGTG TTTGCTGGCC AGTGCTATGC TGAAATTGAG 
GTGATGAAGA TGGTAATGAC CTTAACAGCT GCAGAGTCTG GCTGTATCCA TTATGTCAAA 
CGGCCTGGAG CAGCTCTTGA CCCTGGCTGT GTAATAGCCA AAATGCAGTT GGACAACCCC 
AGCAAGGTCC AACAGGCTGA GCTTCACACA GGTAGTCTGC CACGGATCCA GAGCACTGCA 
CTCAGAGGCG AGAAGCTCCA TCGAGTGTTC CACTATGTCC TGGATAATCT GGTCAACGTA 
ATGAATGGAT ACTGCCTTCC AGATCCTTTT TTTAGCAGCA GGGTAAAAGA CTGGGTAGAG 
CGGTTGATGA AGACCCTCAG AGACCCCTCG TTACCTCTCC TAGAATTACA AGATATCATG 
ACCAGTGTCT CTGGCCGTAT TCCCCCCAAT GTTGAGAAGT CTATCAAGAA GGAAATGGCC 
CAGTATGCCA GCAATATCAC ATCAGTCCTC TGTCAGTTTC CCAGCCAGCA GATTGCCAAC 
ATCCTAGATA GCCATGCAGC TACGTTGAAC CGGAAATCTG AGCGGGAAGT CTTTTTCATG 
AACACTCAGA GCATTGTCCA GCTGGTACAG AGATACCGAA GTGGCATCCG AGGCCACATG 
AAGGCTGTGG TGATGGATCT GCTGCGGCAG TACCTGCGAG TAGAGACACA GTTCCAGAAT 
GGTCACTATG ACAAATGTGT GTTTGCCCTT CGAGAAGAGA ACAAGAGTGA TATGAACACT 
GTACTGAACT ATATCTTCTC TCATGCTCAA GTCACCAAGA AGAATCTTCT GGTCACAATG 
CTTATTGATC AGTTGTGCGG TAGGGACCCT ACTCTCACTG ATGAGCTGCT GAATATTCTC 
ACAGAGCTAA CTCAACTCAG TAAGACCACC AATGCCAAAG TAGCACTTCG AGCACGCCAG 
GTTCTTATTG CCTCCCATTT GCCATCATAT GAGCTTCGCC ATAACCAAGT AGAGTCTATC 
TTCCTTTCAG CTATTGACAT GTATGGACAT CAGTTTTGCA TTGAGAACCT GCAGAAACTC 
ATCTTGTCTG AAACATCTAT TTTTGATGTC CTACCAAACT TCTTCTATCA CAGCAACCAG 
GTAGTGAGGA TGGCAGCTCT GGAGGTTTAT GTTCGAAGGG CTTATATTGC CTATGAACTT 
AACAGCGTAC AACATCGCCA ACTTAAGGAC AACACCTGTG TGGTGGAATT CCAATTCATG 
CTACCCACAT CTCATCCAAA TAGAGGGAAC ATCCCCACGC TAAACAGAAT GTCCTTCTCC 
TCCAACCTCA ACCACTATGG CATGACTCAT GTAGCTAGTG TCAGCGATGT GCTTTTGGAC 
AACTCATTCA CTCCACCATG TCAACGGATG GGCGGAATGG TCTCTTTTCG GACTTTCGAA 
GATTTTGTCA GGATCTTTGA TGAAGTGATG GGCTGCTTCT GTGATTCTCC ACCGCAAAGC 
CCTACATTCC CTGAGGCAGG TCACACATCT CTATACGATG AAGACAAGGT CCCCAGGGAT 
GAACCAATTC ACATTCTGAA TGTGGCTATC AAGACTGACT GTGATATTGA GGATGACAGA 
CTGGCAGCTA TGTTCAGAGA GTTTACCCAA CAAAATAAAG CTACCCTGGT TGAGCATGGG 
ATACGGCGCC TTACTTTCCT GGTTGCACAA AAGAGAGAAT TTCCTAAATT TTTCACCTTC 
CGAGCAAGGG ATAAGTTTGA GGAGGATCGT ATCTATCGTC ACCTGGAACC TGCCCTCGCT 
TTCCAGTTAG AGCTGAACCG GATGAGAAAT TTTGACCTTA CTGCCATTCC ATGTGCTAAT 
CATAAAATGC ATCTGTATCT TGGGGCAGCC AAGGTGGAGG TGGGCACAGA AGTGACAGAC 
TACAGGTTCT TTGTTCGTGC AATCATCAGA CATTCTGATC TGGTCACCAA GGAAGCTTCT 
TTTGAATACC TACAAAATGA AGGGGAGAGG CTGCTTCTAG AAGCCATGGA TGAGTTGGAA 
GTTGCTTTTA ACAATACAAA CGTTCGCACT GACTGTAACC ACATCTTCCT CAACTTTGTG 
CCCACGGTCA TCATGGACCC ATCAAAGATT GAGGAATCTG TGCGGAGCAT GGTAATGCGC 
TATGGAAGTC GGCTGTGGAA ATTACGTGTC CTCCAGGCAG AACTGAAAAT CAACATTCGC 
CTGACACCAA CTGGAAAAGC AATTCCCATC CGCCTCTTCC TGACAAACGA GTCTGGCTAT 
TACTTGGACA TCAGCTTGTA TAAGGAAGTG ACTGACTCCA GGACAGCACA GATCATGTTT 
CAGGCATATG GAGACAAACA GGGACCATTG CATGGAATGT TAATCAACAC TCCATACGTC 
ACCAAAGACC TGCTTCAATC AAAGAGATTC CAGGCACAAT CCTTGGGGAC AACATACATA 
TATGATATAC CAGAGATGTT TCGGCAGTCT CTGATCAAAC TCTGGGAGTC TATGTCCACT 
CAAGCATTTC TTCCATCACC CCCCCTGCCT TCCGACATGC TGACATATAC GGAGCTCGTG 
TTGGATGATC AAGGTCAGCT GGTCCACATG AACAGGCTTC CAGGAGGAAA TGAGATTGGC 
ATGGTAGCGT GGAAAATGAC CCTTAAAAGT CCTGAATACC CAGAAGGCCG AGATATCATT 
GTTATTGGCA ATGACATCAC ATACCGAATT GGGTCCTTTG GGCCCCAGGA GGATTTGCTG 
TTTCTCAGAG CTTCTGAGCT TGCCAGGGCA GAGGGTATCC CACGCATCTT TGTAGCAGCC 
AACAGTGGAG CAAGAATTGG ACTGGCAGAG GAAATTCGTC ATATGTTTCA TGTAGCCTGG 
GTAGATCCTG AGGATCCTTA CAAGGGATAC AAATATTTAT ATCTGACCCC TCAGGATTAT 
AAGAGAGTCA GTGCTCTCAA CTCTGTCCAT TGTGAACATG TAGAGGACGA AGGAGAATCC 
AGGTACAAGA TAACTGATAT TATTGGGAAG GAAGAGGGAC TTGGAGCAGA GAACCTTCGA 
GGCTCTGGAA TGATCGCTGG AGAATCCTCA TTGGCCTATG ATGAGATCAT CACCATCAGC 
CTGGTTACAT GCCGGGCCAT TGGGATTGGA GCTTACCTTG TCCGGCTGGG ACAGAGAACC 
ATCCAGGTTG AAAATTCTCA TTTAATTTTG ACTGGAGCTG GAGCCCTCAA CAAAGTCCTT 
GGGCGGGAAG TATACACCTC CAATAACCAG CTCGGGGGCA TCCAGATCAT GCACAACAAT 
GGGGTGACCC ACAGCATTGT TTGTGATGAC TTTGAAGGGG TTTTCACTGT CCTGCACTGG 
CTATCCTACA TGCCTAAGAA TGTGCGCAGT TTAGTTCCTC TCCTGAACTC CAAGGATCCT 
ATAGATAGAA TCATCGAGTT TGTTCCCACG AAGGCCCCAT ACGATCCTCG ATGGATGCTA 
GCAGGCCGCC CTCACCCAAC TCAGAAAGGT CAGTGGCTGA GTGGATTTTT TGACTATGGC 
TCTTTCTCAG AGATCATGCA ACCCTGGGCA CAAACTGTGG TGGTTGGCAG AGCCAGGCTA 
GGAGGAATAC CTGTGGGAGT AGTTGCTGTA GAAACCCGAA CAGTGGAACT AAGTATCCCA 
GCCGATCCTG CAAACCTGGA TTCTGAAGCC AAGATAATCC AGCAGGCTGG CCAGGTTTGG 
TTCCCAGACT CTGCGTTTAA GACCTATCAG GCTATCAAGG ACTTCAACCG TGAAGGACTG 
CCTCTGATGG TCTTTGCCAA CTGGAGAGGC TTCTCTGGTG GGATGAAAGA TATGTATGAC 
CAAGTGCTGA AGTTTGGTGC CTATATTGTG GATAGCTTAC GGGAGTGCTC CCAGCCTGTG 
CTGGTATACA TTCCTCCTCA GGCTGAGCTC CGGGGTGGCT CCTGGGTTGT GATTGACCCC 
ACCATCAACC CCCGGCATAT GGAGATGTAC GCTGACCGAG AAAGCAGGGG ATCAGTTCTG 
GAGCCAGAAG GGACAGTAGA AATCAAATTC CGCAGAAAGG ATCTGGTGAA AACCATGCGT 
CGGGTGGACC CAGTCTACAT TCGCTTGGCT GAGCGATTGG GCACCCCAGA GCTCAGCGCA 
GCTGAGCGGA AGGAGCTGGA GAACAAGTTG AAGGAGCGGG AGGAATTCCT AATTCCCATT 
TACCATCAGG TAGCCGTGCA GTTTGCTGAC TTGCACGACA CACCAGGCCG GATGCAGGAG 
AAGGGTGTTA TTAATGACAT CCTGGATTGG AAAACATCCC GTACCTTTTT CTATTGGCGG 
CTGAGGCGTC TCCTGCTGGA GGACCTGGTC AAAAAGAAAA TCCACAATGC CAACCCAGAG 
CTGACCGATG GCCAGATCCA GGCCATGCTA CGGCGCTGGT TTGTAGAAGT GGAAGGAACT 
GTGAAGGCCT ACGTTTGGGA CAATAACAAG GACCTGGTGG AGTGGCTGGA GAAACAGCTG 
ACCGAGGAAG ATGGTGTTCG CTCAGTAATC GAGGAAAACA TCAAATATAT CAGCAGAGAC 
TATGTCCTCA AGCAGATCCG CAGCTTGGTA CAGGCCAATC CCGAGGTTGC CATGGACTCC 
ATCGTCCATA TGACTCAGCA TATCTCACCC ACTCAGCGAG CAGAAGTCGT ACGGATCCTC 
TCCACGATGG ACTCCCCTTC AACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_005624780.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624837.1 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X6, mRNA.

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CloneID	OCe12093
Clone ID Related Accession (Same CDS sequence)	XM_005624780.3 , XM_005624780.2 , XM_005624783.2 , XM_005624778.2 , XM_005624779.3 , XM_005624779.2 , XM_005624783.3 , XM_005624778.3
Accession Version	XM_005624780.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	acetyl-CoA carboxylase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624780.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAC CATCCCCCTT GGCCAAACCT CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGTTCTG TGTCTGAAGA TAACTCAGAG GATGAAATTA GCAACCTGGT GAAGCTGGAT 
CTCCTGGAGG AGAAGGAGGG CTCTCTATCA CCAGCTTCTG TTGGCTCAGA TACACTTTCT 
GACTTGGGGA TCTCCAGCCT ACAGGACGGC TTGGCCTTAC ACATGAGGTC CAGCATGTCT 
GGCTTGCATC TAGTGAAGCA AGGTCGAGAC CGAAAGAAAA TAGACTCTCA GCGAGATTTC 
ACTGTAGCTT CTCCAGCAGA ATTTGTTACT CGTTTTGGGG GAAATAAAGT GATTGAGAAG 
GTTCTTATTG CCAACAATGG CATTGCAGCA GTGAAATGCA TGCGGTCTAT CCGTCGATGG 
TCTTATGAGA TGTTTCGAAA TGAACGTGCA ATCCGATTTG TTGTTATGGT AACACCTGAA 
GACCTGAAAG CCAATGCAGA ATACATTAAG ATGGCGGATC ACTATGTGCC AGTGCCAGGA 
GGACCAAATA ACAACAACTA TGCGAACGTG GAATTAATTC TTGATATTGC TAAAAGGATT 
CCCGTACAAG CAGTGTGGGC TGGCTGGGGT CATGCTTCTG AGAATCCCAA GCTCCCAGAA 
CTTCTTTTGA AAAATGGCAT CGCCTTCATG GGCCCTCCAA GCCAGGCCAT GTGGGCTTTG 
GGGGATAAGA TTGCATCTTC TATAGTGGCT CAAACTGCAG GTATCCCAAC TCTTCCCTGG 
AGTGGCAGTG GTCTTCGGGT GGACTGGCAT GAAAATGATT TTTCCAAACG TATCTTAAAT 
GTTCCCCAGG AATTATACGA AAAAGGTTAT GTGAAGGATG TGGATGATGG GCTAAAGGCA 
GCGGAGGAAG TTGGATATCC AGTAATGATC AAGGCCTCAG AAGGAGGAGG AGGGAAAGGA 
ATCAGAAAAG TCAACAATGC AGATGATTTC CCTAACCTCT TCAGACAGGT TCAAGCTGAA 
GTCCCTGGAT CTCCTATATT TGTGATGAGA CTAGCCAAAC AATCTCGTCA TCTGGAGGTA 
CAGATCTTAG CAGATCAATA TGGCAATGCT ATCTCTCTCT TTGGTCGTGA CTGCTCTGTA 
CAGCGCAGGC ATCAGAAGAT TATTGAGGAA GCACCTGCTG CTATTGCTAC TCCAGCAGTA 
TTTGAACATA TGGAACAGTG TGCTGTGAAA CTTGCCAAAA TGGTTGGTTA TGTGAGTGCT 
GGGACTGTGG AATACCTCTA CAGCCAGGAT GGAAGCTTCT ACTTTCTGGA ACTGAACCCT 
CGGCTACAGG TGGAACACCC TTGTACAGAG ATGGTGGCTG ATGTCAACCT CCCTGCAGCA 
CAACTCCAGA TTGCCATGGG GATCCCTCTG TACAGAATCA AGGATATTCG TATGATGTAT 
GGGGTATCTC CCTGGGGTGA TGCTCCCATT GATTTTGAAA ATTCTGCTCA CGTTCCTTGC 
CCAAGGGGCC ATGTTATTGC CGCTCGGATC ACTAGTGAAA ATCCAGATGA GGGTTTTAAG 
CCCAGCTCAG GAACAGTTCA GGAACTGAAT TTCCGTAGCA ATAAGAATGT TTGGGGTTAT 
TTCAGTGTTG CTGCTGCAGG GGGCCTTCAT GAATTTGCTG ATTCTCAGTT TGGTCATTGC 
TTCTCCTGGG GAGAAAACAG AGAGGAAGCA ATTTCAAACA TGGTGGTGGC TTTGAAGGAG 
CTGTCTATTC GGGGTGACTT TCGAACCACA GTTGAATACC TCATCAAATT GTTGGAGACT 
GAAAGCTTTC AGTTGAATAG AATTGACACC GGCTGGCTGG ACAGACTGAT AGCAGAGAAA 
GTACAGGCAG AGCGACCTGA CACCATGTTG GGAGTTGTTT GTGGGGCTCT CCATGTGGCA 
GATGTGAGCC TACGGAATAG CATCTCTAAC TTCCTTCATT CTTTAGAAAG GGGTCAAGTC 
CTTCCTGCTC ATACACTTCT GAATACAGTA GATGTTGAAC TTATCTATGG GGGAGTCAAA 
TATGTACTTA AGGTGACTCG GCAGTCCCCC AACTCCTATG TGGTGATCAT GAATGGTTCA 
TGCGTAGAAG TAGATGTGCA TCGGCTCAGT GATGGAGGGT TACTCCTGTC CTATGATGGC 
AGCAGTTACA CCACATACAT GAAGGAAGAA GTGGATAGAT ATCGTATCAC AATTGGCAAT 
AAGACCTGTG TGTTTGAGAA GGAAAATGAC CCTTCAGTGT TACGCTCACC TTCTGCTGGG 
AAGTTAATCC AGTACATTGT GGAGGATGGA GGCCATGTGT TTGCTGGCCA GTGCTATGCT 
GAAATTGAGG TGATGAAGAT GGTAATGACC TTAACAGCTG CAGAGTCTGG CTGTATCCAT 
TATGTCAAAC GGCCTGGAGC AGCTCTTGAC CCTGGCTGTG TAATAGCCAA AATGCAGTTG 
GACAACCCCA GCAAGGTCCA ACAGGCTGAG CTTCACACAG GTAGTCTGCC ACGGATCCAG 
AGCACTGCAC TCAGAGGCGA GAAGCTCCAT CGAGTGTTCC ACTATGTCCT GGATAATCTG 
GTCAACGTAA TGAATGGATA CTGCCTTCCA GATCCTTTTT TTAGCAGCAG GGTAAAAGAC 
TGGGTAGAGC GGTTGATGAA GACCCTCAGA GACCCCTCGT TACCTCTCCT AGAATTACAA 
GATATCATGA CCAGTGTCTC TGGCCGTATT CCCCCCAATG TTGAGAAGTC TATCAAGAAG 
GAAATGGCCC AGTATGCCAG CAATATCACA TCAGTCCTCT GTCAGTTTCC CAGCCAGCAG 
ATTGCCAACA TCCTAGATAG CCATGCAGCT ACGTTGAACC GGAAATCTGA GCGGGAAGTC 
TTTTTCATGA ACACTCAGAG CATTGTCCAG CTGGTACAGA GATACCGAAG TGGCATCCGA 
GGCCACATGA AGGCTGTGGT GATGGATCTG CTGCGGCAGT ACCTGCGAGT AGAGACACAG 
TTCCAGAATG GTCACTATGA CAAATGTGTG TTTGCCCTTC GAGAAGAGAA CAAGAGTGAT 
ATGAACACTG TACTGAACTA TATCTTCTCT CATGCTCAAG TCACCAAGAA GAATCTTCTG 
GTCACAATGC TTATTGATCA GTTGTGCGGT AGGGACCCTA CTCTCACTGA TGAGCTGCTG 
AATATTCTCA CAGAGCTAAC TCAACTCAGT AAGACCACCA ATGCCAAAGT AGCACTTCGA 
GCACGCCAGG TTCTTATTGC CTCCCATTTG CCATCATATG AGCTTCGCCA TAACCAAGTA 
GAGTCTATCT TCCTTTCAGC TATTGACATG TATGGACATC AGTTTTGCAT TGAGAACCTG 
CAGAAACTCA TCTTGTCTGA AACATCTATT TTTGATGTCC TACCAAACTT CTTCTATCAC 
AGCAACCAGG TAGTGAGGAT GGCAGCTCTG GAGGTTTATG TTCGAAGGGC TTATATTGCC 
TATGAACTTA ACAGCGTACA ACATCGCCAA CTTAAGGACA ACACCTGTGT GGTGGAATTC 
CAATTCATGC TACCCACATC TCATCCAAAT AGAGGGAACA TCCCCACGCT AAACAGAATG 
TCCTTCTCCT CCAACCTCAA CCACTATGGC ATGACTCATG TAGCTAGTGT CAGCGATGTG 
CTTTTGGACA ACTCATTCAC TCCACCATGT CAACGGATGG GCGGAATGGT CTCTTTTCGG 
ACTTTCGAAG ATTTTGTCAG GATCTTTGAT GAAGTGATGG GCTGCTTCTG TGATTCTCCA 
CCGCAAAGCC CTACATTCCC TGAGGCAGGT CACACATCTC TATACGATGA AGACAAGGTC 
CCCAGGGATG AACCAATTCA CATTCTGAAT GTGGCTATCA AGACTGACTG TGATATTGAG 
GATGACAGAC TGGCAGCTAT GTTCAGAGAG TTTACCCAAC AAAATAAAGC TACCCTGGTT 
GAGCATGGGA TACGGCGCCT TACTTTCCTG GTTGCACAAA AGGATTTCAG GAAACAGGTC 
AACTATGAGG TGGATCAGAG ATTTCATAGA GAATTTCCTA AATTTTTCAC CTTCCGAGCA 
AGGGATAAGT TTGAGGAGGA TCGTATCTAT CGTCACCTGG AACCTGCCCT CGCTTTCCAG 
TTAGAGCTGA ACCGGATGAG AAATTTTGAC CTTACTGCCA TTCCATGTGC TAATCATAAA 
ATGCATCTGT ATCTTGGGGC AGCCAAGGTG GAGGTGGGCA CAGAAGTGAC AGACTACAGG 
TTCTTTGTTC GTGCAATCAT CAGACATTCT GATCTGGTCA CCAAGGAAGC TTCTTTTGAA 
TACCTACAAA ATGAAGGGGA GAGGCTGCTT CTAGAAGCCA TGGATGAGTT GGAAGTTGCT 
TTTAACAATA CAAACGTTCG CACTGACTGT AACCACATCT TCCTCAACTT TGTGCCCACG 
GTCATCATGG ACCCATCAAA GATTGAGGAA TCTGTGCGGA GCATGGTAAT GCGCTATGGA 
AGTCGGCTGT GGAAATTACG TGTCCTCCAG GCAGAACTGA AAATCAACAT TCGCCTGACA 
CCAACTGGAA AAGCAATTCC CATCCGCCTC TTCCTGACAA ACGAGTCTGG CTATTACTTG 
GACATCAGCT TGTATAAGGA AGTGACTGAC TCCAGGACAG CACAGATCAT GTTTCAGGCA 
TATGGAGACA AACAGGGACC ATTGCATGGA ATGTTAATCA ACACTCCATA CGTCACCAAA 
GACCTGCTTC AATCAAAGAG ATTCCAGGCA CAATCCTTGG GGACAACATA CATATATGAT 
ATACCAGAGA TGTTTCGGCA GTCTCTGATC AAACTCTGGG AGTCTATGTC CACTCAAGCA 
TTTCTTCCAT CACCCCCCCT GCCTTCCGAC ATGCTGACAT ATACGGAGCT CGTGTTGGAT 
GATCAAGGTC AGCTGGTCCA CATGAACAGG CTTCCAGGAG GAAATGAGAT TGGCATGGTA 
GCGTGGAAAA TGACCCTTAA AAGTCCTGAA TACCCAGAAG GCCGAGATAT CATTGTTATT 
GGCAATGACA TCACATACCG AATTGGGTCC TTTGGGCCCC AGGAGGATTT GCTGTTTCTC 
AGAGCTTCTG AGCTTGCCAG GGCAGAGGGT ATCCCACGCA TCTTTGTAGC AGCCAACAGT 
GGAGCAAGAA TTGGACTGGC AGAGGAAATT CGTCATATGT TTCATGTAGC CTGGGTAGAT 
CCTGAGGATC CTTACAAGGG ATACAAATAT TTATATCTGA CCCCTCAGGA TTATAAGAGA 
GTCAGTGCTC TCAACTCTGT CCATTGTGAA CATGTAGAGG ACGAAGGAGA ATCCAGGTAC 
AAGATAACTG ATATTATTGG GAAGGAAGAG GGACTTGGAG CAGAGAACCT TCGAGGCTCT 
GGAATGATCG CTGGAGAATC CTCATTGGCC TATGATGAGA TCATCACCAT CAGCCTGGTT 
ACATGCCGGG CCATTGGGAT TGGAGCTTAC CTTGTCCGGC TGGGACAGAG AACCATCCAG 
GTTGAAAATT CTCATTTAAT TTTGACTGGA GCTGGAGCCC TCAACAAAGT CCTTGGGCGG 
GAAGTATACA CCTCCAATAA CCAGCTCGGG GGCATCCAGA TCATGCACAA CAATGGGGTG 
ACCCACAGCA TTGTTTGTGA TGACTTTGAA GGGGTTTTCA CTGTCCTGCA CTGGCTATCC 
TACATGCCTA AGAATGTGCG CAGTTTAGTT CCTCTCCTGA ACTCCAAGGA TCCTATAGAT 
AGAATCATCG AGTTTGTTCC CACGAAGGCC CCATACGATC CTCGATGGAT GCTAGCAGGC 
CGCCCTCACC CAACTCAGAA AGGTCAGTGG CTGAGTGGAT TTTTTGACTA TGGCTCTTTC 
TCAGAGATCA TGCAACCCTG GGCACAAACT GTGGTGGTTG GCAGAGCCAG GCTAGGAGGA 
ATACCTGTGG GAGTAGTTGC TGTAGAAACC CGAACAGTGG AACTAAGTAT CCCAGCCGAT 
CCTGCAAACC TGGATTCTGA AGCCAAGATA ATCCAGCAGG CTGGCCAGGT TTGGTTCCCA 
GACTCTGCGT TTAAGACCTA TCAGGCTATC AAGGACTTCA ACCGTGAAGG ACTGCCTCTG 
ATGGTCTTTG CCAACTGGAG AGGCTTCTCT GGTGGGATGA AAGATATGTA TGACCAAGTG 
CTGAAGTTTG GTGCCTATAT TGTGGATAGC TTACGGGAGT GCTCCCAGCC TGTGCTGGTA 
TACATTCCTC CTCAGGCTGA GCTCCGGGGT GGCTCCTGGG TTGTGATTGA CCCCACCATC 
AACCCCCGGC ATATGGAGAT GTACGCTGAC CGAGAAAGCA GGGGATCAGT TCTGGAGCCA 
GAAGGGACAG TAGAAATCAA ATTCCGCAGA AAGGATCTGG TGAAAACCAT GCGTCGGGTG 
GACCCAGTCT ACATTCGCTT GGCTGAGCGA TTGGGCACCC CAGAGCTCAG CGCAGCTGAG 
CGGAAGGAGC TGGAGAACAA GTTGAAGGAG CGGGAGGAAT TCCTAATTCC CATTTACCAT 
CAGGTAGCCG TGCAGTTTGC TGACTTGCAC GACACACCAG GCCGGATGCA GGAGAAGGGT 
GTTATTAATG ACATCCTGGA TTGGAAAACA TCCCGTACCT TTTTCTATTG GCGGCTGAGG 
CGTCTCCTGC TGGAGGACCT GGTCAAAAAG AAAATCCACA ATGCCAACCC AGAGCTGACC 
GATGGCCAGA TCCAGGCCAT GCTACGGCGC TGGTTTGTAG AAGTGGAAGG AACTGTGAAG 
GCCTACGTTT GGGACAATAA CAAGGACCTG GTGGAGTGGC TGGAGAAACA GCTGACCGAG 
GAAGATGGTG TTCGCTCAGT AATCGAGGAA AACATCAAAT ATATCAGCAG AGACTATGTC 
CTCAAGCAGA TCCGCAGCTT GGTACAGGCC AATCCCGAGG TTGCCATGGA CTCCATCGTC 
CATATGACTC AGCATATCTC ACCCACTCAG CGAGCAGAAG TCGTACGGAT CCTCTCCACG 
ATGGACTCCC CTTCAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624837.1
CDS	267..7307
Translation	MDEPSPLAKPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_005624780.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624780.3

ORF Insert Sequence:

ATGGATGAAC CATCCCCCTT GGCCAAACCT CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGTTCTG TGTCTGAAGA TAACTCAGAG GATGAAATTA GCAACCTGGT GAAGCTGGAT 
CTCCTGGAGG AGAAGGAGGG CTCTCTATCA CCAGCTTCTG TTGGCTCAGA TACACTTTCT 
GACTTGGGGA TCTCCAGCCT ACAGGACGGC TTGGCCTTAC ACATGAGGTC CAGCATGTCT 
GGCTTGCATC TAGTGAAGCA AGGTCGAGAC CGAAAGAAAA TAGACTCTCA GCGAGATTTC 
ACTGTAGCTT CTCCAGCAGA ATTTGTTACT CGTTTTGGGG GAAATAAAGT GATTGAGAAG 
GTTCTTATTG CCAACAATGG CATTGCAGCA GTGAAATGCA TGCGGTCTAT CCGTCGATGG 
TCTTATGAGA TGTTTCGAAA TGAACGTGCA ATCCGATTTG TTGTTATGGT AACACCTGAA 
GACCTGAAAG CCAATGCAGA ATACATTAAG ATGGCGGATC ACTATGTGCC AGTGCCAGGA 
GGACCAAATA ACAACAACTA TGCGAACGTG GAATTAATTC TTGATATTGC TAAAAGGATT 
CCCGTACAAG CAGTGTGGGC TGGCTGGGGT CATGCTTCTG AGAATCCCAA GCTCCCAGAA 
CTTCTTTTGA AAAATGGCAT CGCCTTCATG GGCCCTCCAA GCCAGGCCAT GTGGGCTTTG 
GGGGATAAGA TTGCATCTTC TATAGTGGCT CAAACTGCAG GTATCCCAAC TCTTCCCTGG 
AGTGGCAGTG GTCTTCGGGT GGACTGGCAT GAAAATGATT TTTCCAAACG TATCTTAAAT 
GTTCCCCAGG AATTATACGA AAAAGGTTAT GTGAAGGATG TGGATGATGG GCTAAAGGCA 
GCGGAGGAAG TTGGATATCC AGTAATGATC AAGGCCTCAG AAGGAGGAGG AGGGAAAGGA 
ATCAGAAAAG TCAACAATGC AGATGATTTC CCTAACCTCT TCAGACAGGT TCAAGCTGAA 
GTCCCTGGAT CTCCTATATT TGTGATGAGA CTAGCCAAAC AATCTCGTCA TCTGGAGGTA 
CAGATCTTAG CAGATCAATA TGGCAATGCT ATCTCTCTCT TTGGTCGTGA CTGCTCTGTA 
CAGCGCAGGC ATCAGAAGAT TATTGAGGAA GCACCTGCTG CTATTGCTAC TCCAGCAGTA 
TTTGAACATA TGGAACAGTG TGCTGTGAAA CTTGCCAAAA TGGTTGGTTA TGTGAGTGCT 
GGGACTGTGG AATACCTCTA CAGCCAGGAT GGAAGCTTCT ACTTTCTGGA ACTGAACCCT 
CGGCTACAGG TGGAACACCC TTGTACAGAG ATGGTGGCTG ATGTCAACCT CCCTGCAGCA 
CAACTCCAGA TTGCCATGGG GATCCCTCTG TACAGAATCA AGGATATTCG TATGATGTAT 
GGGGTATCTC CCTGGGGTGA TGCTCCCATT GATTTTGAAA ATTCTGCTCA CGTTCCTTGC 
CCAAGGGGCC ATGTTATTGC CGCTCGGATC ACTAGTGAAA ATCCAGATGA GGGTTTTAAG 
CCCAGCTCAG GAACAGTTCA GGAACTGAAT TTCCGTAGCA ATAAGAATGT TTGGGGTTAT 
TTCAGTGTTG CTGCTGCAGG GGGCCTTCAT GAATTTGCTG ATTCTCAGTT TGGTCATTGC 
TTCTCCTGGG GAGAAAACAG AGAGGAAGCA ATTTCAAACA TGGTGGTGGC TTTGAAGGAG 
CTGTCTATTC GGGGTGACTT TCGAACCACA GTTGAATACC TCATCAAATT GTTGGAGACT 
GAAAGCTTTC AGTTGAATAG AATTGACACC GGCTGGCTGG ACAGACTGAT AGCAGAGAAA 
GTACAGGCAG AGCGACCTGA CACCATGTTG GGAGTTGTTT GTGGGGCTCT CCATGTGGCA 
GATGTGAGCC TACGGAATAG CATCTCTAAC TTCCTTCATT CTTTAGAAAG GGGTCAAGTC 
CTTCCTGCTC ATACACTTCT GAATACAGTA GATGTTGAAC TTATCTATGG GGGAGTCAAA 
TATGTACTTA AGGTGACTCG GCAGTCCCCC AACTCCTATG TGGTGATCAT GAATGGTTCA 
TGCGTAGAAG TAGATGTGCA TCGGCTCAGT GATGGAGGGT TACTCCTGTC CTATGATGGC 
AGCAGTTACA CCACATACAT GAAGGAAGAA GTGGATAGAT ATCGTATCAC AATTGGCAAT 
AAGACCTGTG TGTTTGAGAA GGAAAATGAC CCTTCAGTGT TACGCTCACC TTCTGCTGGG 
AAGTTAATCC AGTACATTGT GGAGGATGGA GGCCATGTGT TTGCTGGCCA GTGCTATGCT 
GAAATTGAGG TGATGAAGAT GGTAATGACC TTAACAGCTG CAGAGTCTGG CTGTATCCAT 
TATGTCAAAC GGCCTGGAGC AGCTCTTGAC CCTGGCTGTG TAATAGCCAA AATGCAGTTG 
GACAACCCCA GCAAGGTCCA ACAGGCTGAG CTTCACACAG GTAGTCTGCC ACGGATCCAG 
AGCACTGCAC TCAGAGGCGA GAAGCTCCAT CGAGTGTTCC ACTATGTCCT GGATAATCTG 
GTCAACGTAA TGAATGGATA CTGCCTTCCA GATCCTTTTT TTAGCAGCAG GGTAAAAGAC 
TGGGTAGAGC GGTTGATGAA GACCCTCAGA GACCCCTCGT TACCTCTCCT AGAATTACAA 
GATATCATGA CCAGTGTCTC TGGCCGTATT CCCCCCAATG TTGAGAAGTC TATCAAGAAG 
GAAATGGCCC AGTATGCCAG CAATATCACA TCAGTCCTCT GTCAGTTTCC CAGCCAGCAG 
ATTGCCAACA TCCTAGATAG CCATGCAGCT ACGTTGAACC GGAAATCTGA GCGGGAAGTC 
TTTTTCATGA ACACTCAGAG CATTGTCCAG CTGGTACAGA GATACCGAAG TGGCATCCGA 
GGCCACATGA AGGCTGTGGT GATGGATCTG CTGCGGCAGT ACCTGCGAGT AGAGACACAG 
TTCCAGAATG GTCACTATGA CAAATGTGTG TTTGCCCTTC GAGAAGAGAA CAAGAGTGAT 
ATGAACACTG TACTGAACTA TATCTTCTCT CATGCTCAAG TCACCAAGAA GAATCTTCTG 
GTCACAATGC TTATTGATCA GTTGTGCGGT AGGGACCCTA CTCTCACTGA TGAGCTGCTG 
AATATTCTCA CAGAGCTAAC TCAACTCAGT AAGACCACCA ATGCCAAAGT AGCACTTCGA 
GCACGCCAGG TTCTTATTGC CTCCCATTTG CCATCATATG AGCTTCGCCA TAACCAAGTA 
GAGTCTATCT TCCTTTCAGC TATTGACATG TATGGACATC AGTTTTGCAT TGAGAACCTG 
CAGAAACTCA TCTTGTCTGA AACATCTATT TTTGATGTCC TACCAAACTT CTTCTATCAC 
AGCAACCAGG TAGTGAGGAT GGCAGCTCTG GAGGTTTATG TTCGAAGGGC TTATATTGCC 
TATGAACTTA ACAGCGTACA ACATCGCCAA CTTAAGGACA ACACCTGTGT GGTGGAATTC 
CAATTCATGC TACCCACATC TCATCCAAAT AGAGGGAACA TCCCCACGCT AAACAGAATG 
TCCTTCTCCT CCAACCTCAA CCACTATGGC ATGACTCATG TAGCTAGTGT CAGCGATGTG 
CTTTTGGACA ACTCATTCAC TCCACCATGT CAACGGATGG GCGGAATGGT CTCTTTTCGG 
ACTTTCGAAG ATTTTGTCAG GATCTTTGAT GAAGTGATGG GCTGCTTCTG TGATTCTCCA 
CCGCAAAGCC CTACATTCCC TGAGGCAGGT CACACATCTC TATACGATGA AGACAAGGTC 
CCCAGGGATG AACCAATTCA CATTCTGAAT GTGGCTATCA AGACTGACTG TGATATTGAG 
GATGACAGAC TGGCAGCTAT GTTCAGAGAG TTTACCCAAC AAAATAAAGC TACCCTGGTT 
GAGCATGGGA TACGGCGCCT TACTTTCCTG GTTGCACAAA AGGATTTCAG GAAACAGGTC 
AACTATGAGG TGGATCAGAG ATTTCATAGA GAATTTCCTA AATTTTTCAC CTTCCGAGCA 
AGGGATAAGT TTGAGGAGGA TCGTATCTAT CGTCACCTGG AACCTGCCCT CGCTTTCCAG 
TTAGAGCTGA ACCGGATGAG AAATTTTGAC CTTACTGCCA TTCCATGTGC TAATCATAAA 
ATGCATCTGT ATCTTGGGGC AGCCAAGGTG GAGGTGGGCA CAGAAGTGAC AGACTACAGG 
TTCTTTGTTC GTGCAATCAT CAGACATTCT GATCTGGTCA CCAAGGAAGC TTCTTTTGAA 
TACCTACAAA ATGAAGGGGA GAGGCTGCTT CTAGAAGCCA TGGATGAGTT GGAAGTTGCT 
TTTAACAATA CAAACGTTCG CACTGACTGT AACCACATCT TCCTCAACTT TGTGCCCACG 
GTCATCATGG ACCCATCAAA GATTGAGGAA TCTGTGCGGA GCATGGTAAT GCGCTATGGA 
AGTCGGCTGT GGAAATTACG TGTCCTCCAG GCAGAACTGA AAATCAACAT TCGCCTGACA 
CCAACTGGAA AAGCAATTCC CATCCGCCTC TTCCTGACAA ACGAGTCTGG CTATTACTTG 
GACATCAGCT TGTATAAGGA AGTGACTGAC TCCAGGACAG CACAGATCAT GTTTCAGGCA 
TATGGAGACA AACAGGGACC ATTGCATGGA ATGTTAATCA ACACTCCATA CGTCACCAAA 
GACCTGCTTC AATCAAAGAG ATTCCAGGCA CAATCCTTGG GGACAACATA CATATATGAT 
ATACCAGAGA TGTTTCGGCA GTCTCTGATC AAACTCTGGG AGTCTATGTC CACTCAAGCA 
TTTCTTCCAT CACCCCCCCT GCCTTCCGAC ATGCTGACAT ATACGGAGCT CGTGTTGGAT 
GATCAAGGTC AGCTGGTCCA CATGAACAGG CTTCCAGGAG GAAATGAGAT TGGCATGGTA 
GCGTGGAAAA TGACCCTTAA AAGTCCTGAA TACCCAGAAG GCCGAGATAT CATTGTTATT 
GGCAATGACA TCACATACCG AATTGGGTCC TTTGGGCCCC AGGAGGATTT GCTGTTTCTC 
AGAGCTTCTG AGCTTGCCAG GGCAGAGGGT ATCCCACGCA TCTTTGTAGC AGCCAACAGT 
GGAGCAAGAA TTGGACTGGC AGAGGAAATT CGTCATATGT TTCATGTAGC CTGGGTAGAT 
CCTGAGGATC CTTACAAGGG ATACAAATAT TTATATCTGA CCCCTCAGGA TTATAAGAGA 
GTCAGTGCTC TCAACTCTGT CCATTGTGAA CATGTAGAGG ACGAAGGAGA ATCCAGGTAC 
AAGATAACTG ATATTATTGG GAAGGAAGAG GGACTTGGAG CAGAGAACCT TCGAGGCTCT 
GGAATGATCG CTGGAGAATC CTCATTGGCC TATGATGAGA TCATCACCAT CAGCCTGGTT 
ACATGCCGGG CCATTGGGAT TGGAGCTTAC CTTGTCCGGC TGGGACAGAG AACCATCCAG 
GTTGAAAATT CTCATTTAAT TTTGACTGGA GCTGGAGCCC TCAACAAAGT CCTTGGGCGG 
GAAGTATACA CCTCCAATAA CCAGCTCGGG GGCATCCAGA TCATGCACAA CAATGGGGTG 
ACCCACAGCA TTGTTTGTGA TGACTTTGAA GGGGTTTTCA CTGTCCTGCA CTGGCTATCC 
TACATGCCTA AGAATGTGCG CAGTTTAGTT CCTCTCCTGA ACTCCAAGGA TCCTATAGAT 
AGAATCATCG AGTTTGTTCC CACGAAGGCC CCATACGATC CTCGATGGAT GCTAGCAGGC 
CGCCCTCACC CAACTCAGAA AGGTCAGTGG CTGAGTGGAT TTTTTGACTA TGGCTCTTTC 
TCAGAGATCA TGCAACCCTG GGCACAAACT GTGGTGGTTG GCAGAGCCAG GCTAGGAGGA 
ATACCTGTGG GAGTAGTTGC TGTAGAAACC CGAACAGTGG AACTAAGTAT CCCAGCCGAT 
CCTGCAAACC TGGATTCTGA AGCCAAGATA ATCCAGCAGG CTGGCCAGGT TTGGTTCCCA 
GACTCTGCGT TTAAGACCTA TCAGGCTATC AAGGACTTCA ACCGTGAAGG ACTGCCTCTG 
ATGGTCTTTG CCAACTGGAG AGGCTTCTCT GGTGGGATGA AAGATATGTA TGACCAAGTG 
CTGAAGTTTG GTGCCTATAT TGTGGATAGC TTACGGGAGT GCTCCCAGCC TGTGCTGGTA 
TACATTCCTC CTCAGGCTGA GCTCCGGGGT GGCTCCTGGG TTGTGATTGA CCCCACCATC 
AACCCCCGGC ATATGGAGAT GTACGCTGAC CGAGAAAGCA GGGGATCAGT TCTGGAGCCA 
GAAGGGACAG TAGAAATCAA ATTCCGCAGA AAGGATCTGG TGAAAACCAT GCGTCGGGTG 
GACCCAGTCT ACATTCGCTT GGCTGAGCGA TTGGGCACCC CAGAGCTCAG CGCAGCTGAG 
CGGAAGGAGC TGGAGAACAA GTTGAAGGAG CGGGAGGAAT TCCTAATTCC CATTTACCAT 
CAGGTAGCCG TGCAGTTTGC TGACTTGCAC GACACACCAG GCCGGATGCA GGAGAAGGGT 
GTTATTAATG ACATCCTGGA TTGGAAAACA TCCCGTACCT TTTTCTATTG GCGGCTGAGG 
CGTCTCCTGC TGGAGGACCT GGTCAAAAAG AAAATCCACA ATGCCAACCC AGAGCTGACC 
GATGGCCAGA TCCAGGCCAT GCTACGGCGC TGGTTTGTAG AAGTGGAAGG AACTGTGAAG 
GCCTACGTTT GGGACAATAA CAAGGACCTG GTGGAGTGGC TGGAGAAACA GCTGACCGAG 
GAAGATGGTG TTCGCTCAGT AATCGAGGAA AACATCAAAT ATATCAGCAG AGACTATGTC 
CTCAAGCAGA TCCGCAGCTT GGTACAGGCC AATCCCGAGG TTGCCATGGA CTCCATCGTC 
CATATGACTC AGCATATCTC ACCCACTCAG CGAGCAGAAG TCGTACGGAT CCTCTCCACG 
ATGGACTCCC CTTCAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_005624780.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624837.1 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X7, mRNA.

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CloneID	OCe12093
Clone ID Related Accession (Same CDS sequence)	XM_005624780.3 , XM_005624780.2 , XM_005624783.2 , XM_005624778.2 , XM_005624779.3 , XM_005624779.2 , XM_005624783.3 , XM_005624778.3
Accession Version	XM_005624780.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	acetyl-CoA carboxylase 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624780.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAC CATCCCCCTT GGCCAAACCT CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGTTCTG TGTCTGAAGA TAACTCAGAG GATGAAATTA GCAACCTGGT GAAGCTGGAT 
CTCCTGGAGG AGAAGGAGGG CTCTCTATCA CCAGCTTCTG TTGGCTCAGA TACACTTTCT 
GACTTGGGGA TCTCCAGCCT ACAGGACGGC TTGGCCTTAC ACATGAGGTC CAGCATGTCT 
GGCTTGCATC TAGTGAAGCA AGGTCGAGAC CGAAAGAAAA TAGACTCTCA GCGAGATTTC 
ACTGTAGCTT CTCCAGCAGA ATTTGTTACT CGTTTTGGGG GAAATAAAGT GATTGAGAAG 
GTTCTTATTG CCAACAATGG CATTGCAGCA GTGAAATGCA TGCGGTCTAT CCGTCGATGG 
TCTTATGAGA TGTTTCGAAA TGAACGTGCA ATCCGATTTG TTGTTATGGT AACACCTGAA 
GACCTGAAAG CCAATGCAGA ATACATTAAG ATGGCGGATC ACTATGTGCC AGTGCCAGGA 
GGACCAAATA ACAACAACTA TGCGAACGTG GAATTAATTC TTGATATTGC TAAAAGGATT 
CCCGTACAAG CAGTGTGGGC TGGCTGGGGT CATGCTTCTG AGAATCCCAA GCTCCCAGAA 
CTTCTTTTGA AAAATGGCAT CGCCTTCATG GGCCCTCCAA GCCAGGCCAT GTGGGCTTTG 
GGGGATAAGA TTGCATCTTC TATAGTGGCT CAAACTGCAG GTATCCCAAC TCTTCCCTGG 
AGTGGCAGTG GTCTTCGGGT GGACTGGCAT GAAAATGATT TTTCCAAACG TATCTTAAAT 
GTTCCCCAGG AATTATACGA AAAAGGTTAT GTGAAGGATG TGGATGATGG GCTAAAGGCA 
GCGGAGGAAG TTGGATATCC AGTAATGATC AAGGCCTCAG AAGGAGGAGG AGGGAAAGGA 
ATCAGAAAAG TCAACAATGC AGATGATTTC CCTAACCTCT TCAGACAGGT TCAAGCTGAA 
GTCCCTGGAT CTCCTATATT TGTGATGAGA CTAGCCAAAC AATCTCGTCA TCTGGAGGTA 
CAGATCTTAG CAGATCAATA TGGCAATGCT ATCTCTCTCT TTGGTCGTGA CTGCTCTGTA 
CAGCGCAGGC ATCAGAAGAT TATTGAGGAA GCACCTGCTG CTATTGCTAC TCCAGCAGTA 
TTTGAACATA TGGAACAGTG TGCTGTGAAA CTTGCCAAAA TGGTTGGTTA TGTGAGTGCT 
GGGACTGTGG AATACCTCTA CAGCCAGGAT GGAAGCTTCT ACTTTCTGGA ACTGAACCCT 
CGGCTACAGG TGGAACACCC TTGTACAGAG ATGGTGGCTG ATGTCAACCT CCCTGCAGCA 
CAACTCCAGA TTGCCATGGG GATCCCTCTG TACAGAATCA AGGATATTCG TATGATGTAT 
GGGGTATCTC CCTGGGGTGA TGCTCCCATT GATTTTGAAA ATTCTGCTCA CGTTCCTTGC 
CCAAGGGGCC ATGTTATTGC CGCTCGGATC ACTAGTGAAA ATCCAGATGA GGGTTTTAAG 
CCCAGCTCAG GAACAGTTCA GGAACTGAAT TTCCGTAGCA ATAAGAATGT TTGGGGTTAT 
TTCAGTGTTG CTGCTGCAGG GGGCCTTCAT GAATTTGCTG ATTCTCAGTT TGGTCATTGC 
TTCTCCTGGG GAGAAAACAG AGAGGAAGCA ATTTCAAACA TGGTGGTGGC TTTGAAGGAG 
CTGTCTATTC GGGGTGACTT TCGAACCACA GTTGAATACC TCATCAAATT GTTGGAGACT 
GAAAGCTTTC AGTTGAATAG AATTGACACC GGCTGGCTGG ACAGACTGAT AGCAGAGAAA 
GTACAGGCAG AGCGACCTGA CACCATGTTG GGAGTTGTTT GTGGGGCTCT CCATGTGGCA 
GATGTGAGCC TACGGAATAG CATCTCTAAC TTCCTTCATT CTTTAGAAAG GGGTCAAGTC 
CTTCCTGCTC ATACACTTCT GAATACAGTA GATGTTGAAC TTATCTATGG GGGAGTCAAA 
TATGTACTTA AGGTGACTCG GCAGTCCCCC AACTCCTATG TGGTGATCAT GAATGGTTCA 
TGCGTAGAAG TAGATGTGCA TCGGCTCAGT GATGGAGGGT TACTCCTGTC CTATGATGGC 
AGCAGTTACA CCACATACAT GAAGGAAGAA GTGGATAGAT ATCGTATCAC AATTGGCAAT 
AAGACCTGTG TGTTTGAGAA GGAAAATGAC CCTTCAGTGT TACGCTCACC TTCTGCTGGG 
AAGTTAATCC AGTACATTGT GGAGGATGGA GGCCATGTGT TTGCTGGCCA GTGCTATGCT 
GAAATTGAGG TGATGAAGAT GGTAATGACC TTAACAGCTG CAGAGTCTGG CTGTATCCAT 
TATGTCAAAC GGCCTGGAGC AGCTCTTGAC CCTGGCTGTG TAATAGCCAA AATGCAGTTG 
GACAACCCCA GCAAGGTCCA ACAGGCTGAG CTTCACACAG GTAGTCTGCC ACGGATCCAG 
AGCACTGCAC TCAGAGGCGA GAAGCTCCAT CGAGTGTTCC ACTATGTCCT GGATAATCTG 
GTCAACGTAA TGAATGGATA CTGCCTTCCA GATCCTTTTT TTAGCAGCAG GGTAAAAGAC 
TGGGTAGAGC GGTTGATGAA GACCCTCAGA GACCCCTCGT TACCTCTCCT AGAATTACAA 
GATATCATGA CCAGTGTCTC TGGCCGTATT CCCCCCAATG TTGAGAAGTC TATCAAGAAG 
GAAATGGCCC AGTATGCCAG CAATATCACA TCAGTCCTCT GTCAGTTTCC CAGCCAGCAG 
ATTGCCAACA TCCTAGATAG CCATGCAGCT ACGTTGAACC GGAAATCTGA GCGGGAAGTC 
TTTTTCATGA ACACTCAGAG CATTGTCCAG CTGGTACAGA GATACCGAAG TGGCATCCGA 
GGCCACATGA AGGCTGTGGT GATGGATCTG CTGCGGCAGT ACCTGCGAGT AGAGACACAG 
TTCCAGAATG GTCACTATGA CAAATGTGTG TTTGCCCTTC GAGAAGAGAA CAAGAGTGAT 
ATGAACACTG TACTGAACTA TATCTTCTCT CATGCTCAAG TCACCAAGAA GAATCTTCTG 
GTCACAATGC TTATTGATCA GTTGTGCGGT AGGGACCCTA CTCTCACTGA TGAGCTGCTG 
AATATTCTCA CAGAGCTAAC TCAACTCAGT AAGACCACCA ATGCCAAAGT AGCACTTCGA 
GCACGCCAGG TTCTTATTGC CTCCCATTTG CCATCATATG AGCTTCGCCA TAACCAAGTA 
GAGTCTATCT TCCTTTCAGC TATTGACATG TATGGACATC AGTTTTGCAT TGAGAACCTG 
CAGAAACTCA TCTTGTCTGA AACATCTATT TTTGATGTCC TACCAAACTT CTTCTATCAC 
AGCAACCAGG TAGTGAGGAT GGCAGCTCTG GAGGTTTATG TTCGAAGGGC TTATATTGCC 
TATGAACTTA ACAGCGTACA ACATCGCCAA CTTAAGGACA ACACCTGTGT GGTGGAATTC 
CAATTCATGC TACCCACATC TCATCCAAAT AGAGGGAACA TCCCCACGCT AAACAGAATG 
TCCTTCTCCT CCAACCTCAA CCACTATGGC ATGACTCATG TAGCTAGTGT CAGCGATGTG 
CTTTTGGACA ACTCATTCAC TCCACCATGT CAACGGATGG GCGGAATGGT CTCTTTTCGG 
ACTTTCGAAG ATTTTGTCAG GATCTTTGAT GAAGTGATGG GCTGCTTCTG TGATTCTCCA 
CCGCAAAGCC CTACATTCCC TGAGGCAGGT CACACATCTC TATACGATGA AGACAAGGTC 
CCCAGGGATG AACCAATTCA CATTCTGAAT GTGGCTATCA AGACTGACTG TGATATTGAG 
GATGACAGAC TGGCAGCTAT GTTCAGAGAG TTTACCCAAC AAAATAAAGC TACCCTGGTT 
GAGCATGGGA TACGGCGCCT TACTTTCCTG GTTGCACAAA AGGATTTCAG GAAACAGGTC 
AACTATGAGG TGGATCAGAG ATTTCATAGA GAATTTCCTA AATTTTTCAC CTTCCGAGCA 
AGGGATAAGT TTGAGGAGGA TCGTATCTAT CGTCACCTGG AACCTGCCCT CGCTTTCCAG 
TTAGAGCTGA ACCGGATGAG AAATTTTGAC CTTACTGCCA TTCCATGTGC TAATCATAAA 
ATGCATCTGT ATCTTGGGGC AGCCAAGGTG GAGGTGGGCA CAGAAGTGAC AGACTACAGG 
TTCTTTGTTC GTGCAATCAT CAGACATTCT GATCTGGTCA CCAAGGAAGC TTCTTTTGAA 
TACCTACAAA ATGAAGGGGA GAGGCTGCTT CTAGAAGCCA TGGATGAGTT GGAAGTTGCT 
TTTAACAATA CAAACGTTCG CACTGACTGT AACCACATCT TCCTCAACTT TGTGCCCACG 
GTCATCATGG ACCCATCAAA GATTGAGGAA TCTGTGCGGA GCATGGTAAT GCGCTATGGA 
AGTCGGCTGT GGAAATTACG TGTCCTCCAG GCAGAACTGA AAATCAACAT TCGCCTGACA 
CCAACTGGAA AAGCAATTCC CATCCGCCTC TTCCTGACAA ACGAGTCTGG CTATTACTTG 
GACATCAGCT TGTATAAGGA AGTGACTGAC TCCAGGACAG CACAGATCAT GTTTCAGGCA 
TATGGAGACA AACAGGGACC ATTGCATGGA ATGTTAATCA ACACTCCATA CGTCACCAAA 
GACCTGCTTC AATCAAAGAG ATTCCAGGCA CAATCCTTGG GGACAACATA CATATATGAT 
ATACCAGAGA TGTTTCGGCA GTCTCTGATC AAACTCTGGG AGTCTATGTC CACTCAAGCA 
TTTCTTCCAT CACCCCCCCT GCCTTCCGAC ATGCTGACAT ATACGGAGCT CGTGTTGGAT 
GATCAAGGTC AGCTGGTCCA CATGAACAGG CTTCCAGGAG GAAATGAGAT TGGCATGGTA 
GCGTGGAAAA TGACCCTTAA AAGTCCTGAA TACCCAGAAG GCCGAGATAT CATTGTTATT 
GGCAATGACA TCACATACCG AATTGGGTCC TTTGGGCCCC AGGAGGATTT GCTGTTTCTC 
AGAGCTTCTG AGCTTGCCAG GGCAGAGGGT ATCCCACGCA TCTTTGTAGC AGCCAACAGT 
GGAGCAAGAA TTGGACTGGC AGAGGAAATT CGTCATATGT TTCATGTAGC CTGGGTAGAT 
CCTGAGGATC CTTACAAGGG ATACAAATAT TTATATCTGA CCCCTCAGGA TTATAAGAGA 
GTCAGTGCTC TCAACTCTGT CCATTGTGAA CATGTAGAGG ACGAAGGAGA ATCCAGGTAC 
AAGATAACTG ATATTATTGG GAAGGAAGAG GGACTTGGAG CAGAGAACCT TCGAGGCTCT 
GGAATGATCG CTGGAGAATC CTCATTGGCC TATGATGAGA TCATCACCAT CAGCCTGGTT 
ACATGCCGGG CCATTGGGAT TGGAGCTTAC CTTGTCCGGC TGGGACAGAG AACCATCCAG 
GTTGAAAATT CTCATTTAAT TTTGACTGGA GCTGGAGCCC TCAACAAAGT CCTTGGGCGG 
GAAGTATACA CCTCCAATAA CCAGCTCGGG GGCATCCAGA TCATGCACAA CAATGGGGTG 
ACCCACAGCA TTGTTTGTGA TGACTTTGAA GGGGTTTTCA CTGTCCTGCA CTGGCTATCC 
TACATGCCTA AGAATGTGCG CAGTTTAGTT CCTCTCCTGA ACTCCAAGGA TCCTATAGAT 
AGAATCATCG AGTTTGTTCC CACGAAGGCC CCATACGATC CTCGATGGAT GCTAGCAGGC 
CGCCCTCACC CAACTCAGAA AGGTCAGTGG CTGAGTGGAT TTTTTGACTA TGGCTCTTTC 
TCAGAGATCA TGCAACCCTG GGCACAAACT GTGGTGGTTG GCAGAGCCAG GCTAGGAGGA 
ATACCTGTGG GAGTAGTTGC TGTAGAAACC CGAACAGTGG AACTAAGTAT CCCAGCCGAT 
CCTGCAAACC TGGATTCTGA AGCCAAGATA ATCCAGCAGG CTGGCCAGGT TTGGTTCCCA 
GACTCTGCGT TTAAGACCTA TCAGGCTATC AAGGACTTCA ACCGTGAAGG ACTGCCTCTG 
ATGGTCTTTG CCAACTGGAG AGGCTTCTCT GGTGGGATGA AAGATATGTA TGACCAAGTG 
CTGAAGTTTG GTGCCTATAT TGTGGATAGC TTACGGGAGT GCTCCCAGCC TGTGCTGGTA 
TACATTCCTC CTCAGGCTGA GCTCCGGGGT GGCTCCTGGG TTGTGATTGA CCCCACCATC 
AACCCCCGGC ATATGGAGAT GTACGCTGAC CGAGAAAGCA GGGGATCAGT TCTGGAGCCA 
GAAGGGACAG TAGAAATCAA ATTCCGCAGA AAGGATCTGG TGAAAACCAT GCGTCGGGTG 
GACCCAGTCT ACATTCGCTT GGCTGAGCGA TTGGGCACCC CAGAGCTCAG CGCAGCTGAG 
CGGAAGGAGC TGGAGAACAA GTTGAAGGAG CGGGAGGAAT TCCTAATTCC CATTTACCAT 
CAGGTAGCCG TGCAGTTTGC TGACTTGCAC GACACACCAG GCCGGATGCA GGAGAAGGGT 
GTTATTAATG ACATCCTGGA TTGGAAAACA TCCCGTACCT TTTTCTATTG GCGGCTGAGG 
CGTCTCCTGC TGGAGGACCT GGTCAAAAAG AAAATCCACA ATGCCAACCC AGAGCTGACC 
GATGGCCAGA TCCAGGCCAT GCTACGGCGC TGGTTTGTAG AAGTGGAAGG AACTGTGAAG 
GCCTACGTTT GGGACAATAA CAAGGACCTG GTGGAGTGGC TGGAGAAACA GCTGACCGAG 
GAAGATGGTG TTCGCTCAGT AATCGAGGAA AACATCAAAT ATATCAGCAG AGACTATGTC 
CTCAAGCAGA TCCGCAGCTT GGTACAGGCC AATCCCGAGG TTGCCATGGA CTCCATCGTC 
CATATGACTC AGCATATCTC ACCCACTCAG CGAGCAGAAG TCGTACGGAT CCTCTCCACG 
ATGGACTCCC CTTCAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624837.1
CDS	123..7163
Translation	MDEPSPLAKPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_005624780.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624780.2

ORF Insert Sequence:

ATGGATGAAC CATCCCCCTT GGCCAAACCT CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGTTCTG TGTCTGAAGA TAACTCAGAG GATGAAATTA GCAACCTGGT GAAGCTGGAT 
CTCCTGGAGG AGAAGGAGGG CTCTCTATCA CCAGCTTCTG TTGGCTCAGA TACACTTTCT 
GACTTGGGGA TCTCCAGCCT ACAGGACGGC TTGGCCTTAC ACATGAGGTC CAGCATGTCT 
GGCTTGCATC TAGTGAAGCA AGGTCGAGAC CGAAAGAAAA TAGACTCTCA GCGAGATTTC 
ACTGTAGCTT CTCCAGCAGA ATTTGTTACT CGTTTTGGGG GAAATAAAGT GATTGAGAAG 
GTTCTTATTG CCAACAATGG CATTGCAGCA GTGAAATGCA TGCGGTCTAT CCGTCGATGG 
TCTTATGAGA TGTTTCGAAA TGAACGTGCA ATCCGATTTG TTGTTATGGT AACACCTGAA 
GACCTGAAAG CCAATGCAGA ATACATTAAG ATGGCGGATC ACTATGTGCC AGTGCCAGGA 
GGACCAAATA ACAACAACTA TGCGAACGTG GAATTAATTC TTGATATTGC TAAAAGGATT 
CCCGTACAAG CAGTGTGGGC TGGCTGGGGT CATGCTTCTG AGAATCCCAA GCTCCCAGAA 
CTTCTTTTGA AAAATGGCAT CGCCTTCATG GGCCCTCCAA GCCAGGCCAT GTGGGCTTTG 
GGGGATAAGA TTGCATCTTC TATAGTGGCT CAAACTGCAG GTATCCCAAC TCTTCCCTGG 
AGTGGCAGTG GTCTTCGGGT GGACTGGCAT GAAAATGATT TTTCCAAACG TATCTTAAAT 
GTTCCCCAGG AATTATACGA AAAAGGTTAT GTGAAGGATG TGGATGATGG GCTAAAGGCA 
GCGGAGGAAG TTGGATATCC AGTAATGATC AAGGCCTCAG AAGGAGGAGG AGGGAAAGGA 
ATCAGAAAAG TCAACAATGC AGATGATTTC CCTAACCTCT TCAGACAGGT TCAAGCTGAA 
GTCCCTGGAT CTCCTATATT TGTGATGAGA CTAGCCAAAC AATCTCGTCA TCTGGAGGTA 
CAGATCTTAG CAGATCAATA TGGCAATGCT ATCTCTCTCT TTGGTCGTGA CTGCTCTGTA 
CAGCGCAGGC ATCAGAAGAT TATTGAGGAA GCACCTGCTG CTATTGCTAC TCCAGCAGTA 
TTTGAACATA TGGAACAGTG TGCTGTGAAA CTTGCCAAAA TGGTTGGTTA TGTGAGTGCT 
GGGACTGTGG AATACCTCTA CAGCCAGGAT GGAAGCTTCT ACTTTCTGGA ACTGAACCCT 
CGGCTACAGG TGGAACACCC TTGTACAGAG ATGGTGGCTG ATGTCAACCT CCCTGCAGCA 
CAACTCCAGA TTGCCATGGG GATCCCTCTG TACAGAATCA AGGATATTCG TATGATGTAT 
GGGGTATCTC CCTGGGGTGA TGCTCCCATT GATTTTGAAA ATTCTGCTCA CGTTCCTTGC 
CCAAGGGGCC ATGTTATTGC CGCTCGGATC ACTAGTGAAA ATCCAGATGA GGGTTTTAAG 
CCCAGCTCAG GAACAGTTCA GGAACTGAAT TTCCGTAGCA ATAAGAATGT TTGGGGTTAT 
TTCAGTGTTG CTGCTGCAGG GGGCCTTCAT GAATTTGCTG ATTCTCAGTT TGGTCATTGC 
TTCTCCTGGG GAGAAAACAG AGAGGAAGCA ATTTCAAACA TGGTGGTGGC TTTGAAGGAG 
CTGTCTATTC GGGGTGACTT TCGAACCACA GTTGAATACC TCATCAAATT GTTGGAGACT 
GAAAGCTTTC AGTTGAATAG AATTGACACC GGCTGGCTGG ACAGACTGAT AGCAGAGAAA 
GTACAGGCAG AGCGACCTGA CACCATGTTG GGAGTTGTTT GTGGGGCTCT CCATGTGGCA 
GATGTGAGCC TACGGAATAG CATCTCTAAC TTCCTTCATT CTTTAGAAAG GGGTCAAGTC 
CTTCCTGCTC ATACACTTCT GAATACAGTA GATGTTGAAC TTATCTATGG GGGAGTCAAA 
TATGTACTTA AGGTGACTCG GCAGTCCCCC AACTCCTATG TGGTGATCAT GAATGGTTCA 
TGCGTAGAAG TAGATGTGCA TCGGCTCAGT GATGGAGGGT TACTCCTGTC CTATGATGGC 
AGCAGTTACA CCACATACAT GAAGGAAGAA GTGGATAGAT ATCGTATCAC AATTGGCAAT 
AAGACCTGTG TGTTTGAGAA GGAAAATGAC CCTTCAGTGT TACGCTCACC TTCTGCTGGG 
AAGTTAATCC AGTACATTGT GGAGGATGGA GGCCATGTGT TTGCTGGCCA GTGCTATGCT 
GAAATTGAGG TGATGAAGAT GGTAATGACC TTAACAGCTG CAGAGTCTGG CTGTATCCAT 
TATGTCAAAC GGCCTGGAGC AGCTCTTGAC CCTGGCTGTG TAATAGCCAA AATGCAGTTG 
GACAACCCCA GCAAGGTCCA ACAGGCTGAG CTTCACACAG GTAGTCTGCC ACGGATCCAG 
AGCACTGCAC TCAGAGGCGA GAAGCTCCAT CGAGTGTTCC ACTATGTCCT GGATAATCTG 
GTCAACGTAA TGAATGGATA CTGCCTTCCA GATCCTTTTT TTAGCAGCAG GGTAAAAGAC 
TGGGTAGAGC GGTTGATGAA GACCCTCAGA GACCCCTCGT TACCTCTCCT AGAATTACAA 
GATATCATGA CCAGTGTCTC TGGCCGTATT CCCCCCAATG TTGAGAAGTC TATCAAGAAG 
GAAATGGCCC AGTATGCCAG CAATATCACA TCAGTCCTCT GTCAGTTTCC CAGCCAGCAG 
ATTGCCAACA TCCTAGATAG CCATGCAGCT ACGTTGAACC GGAAATCTGA GCGGGAAGTC 
TTTTTCATGA ACACTCAGAG CATTGTCCAG CTGGTACAGA GATACCGAAG TGGCATCCGA 
GGCCACATGA AGGCTGTGGT GATGGATCTG CTGCGGCAGT ACCTGCGAGT AGAGACACAG 
TTCCAGAATG GTCACTATGA CAAATGTGTG TTTGCCCTTC GAGAAGAGAA CAAGAGTGAT 
ATGAACACTG TACTGAACTA TATCTTCTCT CATGCTCAAG TCACCAAGAA GAATCTTCTG 
GTCACAATGC TTATTGATCA GTTGTGCGGT AGGGACCCTA CTCTCACTGA TGAGCTGCTG 
AATATTCTCA CAGAGCTAAC TCAACTCAGT AAGACCACCA ATGCCAAAGT AGCACTTCGA 
GCACGCCAGG TTCTTATTGC CTCCCATTTG CCATCATATG AGCTTCGCCA TAACCAAGTA 
GAGTCTATCT TCCTTTCAGC TATTGACATG TATGGACATC AGTTTTGCAT TGAGAACCTG 
CAGAAACTCA TCTTGTCTGA AACATCTATT TTTGATGTCC TACCAAACTT CTTCTATCAC 
AGCAACCAGG TAGTGAGGAT GGCAGCTCTG GAGGTTTATG TTCGAAGGGC TTATATTGCC 
TATGAACTTA ACAGCGTACA ACATCGCCAA CTTAAGGACA ACACCTGTGT GGTGGAATTC 
CAATTCATGC TACCCACATC TCATCCAAAT AGAGGGAACA TCCCCACGCT AAACAGAATG 
TCCTTCTCCT CCAACCTCAA CCACTATGGC ATGACTCATG TAGCTAGTGT CAGCGATGTG 
CTTTTGGACA ACTCATTCAC TCCACCATGT CAACGGATGG GCGGAATGGT CTCTTTTCGG 
ACTTTCGAAG ATTTTGTCAG GATCTTTGAT GAAGTGATGG GCTGCTTCTG TGATTCTCCA 
CCGCAAAGCC CTACATTCCC TGAGGCAGGT CACACATCTC TATACGATGA AGACAAGGTC 
CCCAGGGATG AACCAATTCA CATTCTGAAT GTGGCTATCA AGACTGACTG TGATATTGAG 
GATGACAGAC TGGCAGCTAT GTTCAGAGAG TTTACCCAAC AAAATAAAGC TACCCTGGTT 
GAGCATGGGA TACGGCGCCT TACTTTCCTG GTTGCACAAA AGGATTTCAG GAAACAGGTC 
AACTATGAGG TGGATCAGAG ATTTCATAGA GAATTTCCTA AATTTTTCAC CTTCCGAGCA 
AGGGATAAGT TTGAGGAGGA TCGTATCTAT CGTCACCTGG AACCTGCCCT CGCTTTCCAG 
TTAGAGCTGA ACCGGATGAG AAATTTTGAC CTTACTGCCA TTCCATGTGC TAATCATAAA 
ATGCATCTGT ATCTTGGGGC AGCCAAGGTG GAGGTGGGCA CAGAAGTGAC AGACTACAGG 
TTCTTTGTTC GTGCAATCAT CAGACATTCT GATCTGGTCA CCAAGGAAGC TTCTTTTGAA 
TACCTACAAA ATGAAGGGGA GAGGCTGCTT CTAGAAGCCA TGGATGAGTT GGAAGTTGCT 
TTTAACAATA CAAACGTTCG CACTGACTGT AACCACATCT TCCTCAACTT TGTGCCCACG 
GTCATCATGG ACCCATCAAA GATTGAGGAA TCTGTGCGGA GCATGGTAAT GCGCTATGGA 
AGTCGGCTGT GGAAATTACG TGTCCTCCAG GCAGAACTGA AAATCAACAT TCGCCTGACA 
CCAACTGGAA AAGCAATTCC CATCCGCCTC TTCCTGACAA ACGAGTCTGG CTATTACTTG 
GACATCAGCT TGTATAAGGA AGTGACTGAC TCCAGGACAG CACAGATCAT GTTTCAGGCA 
TATGGAGACA AACAGGGACC ATTGCATGGA ATGTTAATCA ACACTCCATA CGTCACCAAA 
GACCTGCTTC AATCAAAGAG ATTCCAGGCA CAATCCTTGG GGACAACATA CATATATGAT 
ATACCAGAGA TGTTTCGGCA GTCTCTGATC AAACTCTGGG AGTCTATGTC CACTCAAGCA 
TTTCTTCCAT CACCCCCCCT GCCTTCCGAC ATGCTGACAT ATACGGAGCT CGTGTTGGAT 
GATCAAGGTC AGCTGGTCCA CATGAACAGG CTTCCAGGAG GAAATGAGAT TGGCATGGTA 
GCGTGGAAAA TGACCCTTAA AAGTCCTGAA TACCCAGAAG GCCGAGATAT CATTGTTATT 
GGCAATGACA TCACATACCG AATTGGGTCC TTTGGGCCCC AGGAGGATTT GCTGTTTCTC 
AGAGCTTCTG AGCTTGCCAG GGCAGAGGGT ATCCCACGCA TCTTTGTAGC AGCCAACAGT 
GGAGCAAGAA TTGGACTGGC AGAGGAAATT CGTCATATGT TTCATGTAGC CTGGGTAGAT 
CCTGAGGATC CTTACAAGGG ATACAAATAT TTATATCTGA CCCCTCAGGA TTATAAGAGA 
GTCAGTGCTC TCAACTCTGT CCATTGTGAA CATGTAGAGG ACGAAGGAGA ATCCAGGTAC 
AAGATAACTG ATATTATTGG GAAGGAAGAG GGACTTGGAG CAGAGAACCT TCGAGGCTCT 
GGAATGATCG CTGGAGAATC CTCATTGGCC TATGATGAGA TCATCACCAT CAGCCTGGTT 
ACATGCCGGG CCATTGGGAT TGGAGCTTAC CTTGTCCGGC TGGGACAGAG AACCATCCAG 
GTTGAAAATT CTCATTTAAT TTTGACTGGA GCTGGAGCCC TCAACAAAGT CCTTGGGCGG 
GAAGTATACA CCTCCAATAA CCAGCTCGGG GGCATCCAGA TCATGCACAA CAATGGGGTG 
ACCCACAGCA TTGTTTGTGA TGACTTTGAA GGGGTTTTCA CTGTCCTGCA CTGGCTATCC 
TACATGCCTA AGAATGTGCG CAGTTTAGTT CCTCTCCTGA ACTCCAAGGA TCCTATAGAT 
AGAATCATCG AGTTTGTTCC CACGAAGGCC CCATACGATC CTCGATGGAT GCTAGCAGGC 
CGCCCTCACC CAACTCAGAA AGGTCAGTGG CTGAGTGGAT TTTTTGACTA TGGCTCTTTC 
TCAGAGATCA TGCAACCCTG GGCACAAACT GTGGTGGTTG GCAGAGCCAG GCTAGGAGGA 
ATACCTGTGG GAGTAGTTGC TGTAGAAACC CGAACAGTGG AACTAAGTAT CCCAGCCGAT 
CCTGCAAACC TGGATTCTGA AGCCAAGATA ATCCAGCAGG CTGGCCAGGT TTGGTTCCCA 
GACTCTGCGT TTAAGACCTA TCAGGCTATC AAGGACTTCA ACCGTGAAGG ACTGCCTCTG 
ATGGTCTTTG CCAACTGGAG AGGCTTCTCT GGTGGGATGA AAGATATGTA TGACCAAGTG 
CTGAAGTTTG GTGCCTATAT TGTGGATAGC TTACGGGAGT GCTCCCAGCC TGTGCTGGTA 
TACATTCCTC CTCAGGCTGA GCTCCGGGGT GGCTCCTGGG TTGTGATTGA CCCCACCATC 
AACCCCCGGC ATATGGAGAT GTACGCTGAC CGAGAAAGCA GGGGATCAGT TCTGGAGCCA 
GAAGGGACAG TAGAAATCAA ATTCCGCAGA AAGGATCTGG TGAAAACCAT GCGTCGGGTG 
GACCCAGTCT ACATTCGCTT GGCTGAGCGA TTGGGCACCC CAGAGCTCAG CGCAGCTGAG 
CGGAAGGAGC TGGAGAACAA GTTGAAGGAG CGGGAGGAAT TCCTAATTCC CATTTACCAT 
CAGGTAGCCG TGCAGTTTGC TGACTTGCAC GACACACCAG GCCGGATGCA GGAGAAGGGT 
GTTATTAATG ACATCCTGGA TTGGAAAACA TCCCGTACCT TTTTCTATTG GCGGCTGAGG 
CGTCTCCTGC TGGAGGACCT GGTCAAAAAG AAAATCCACA ATGCCAACCC AGAGCTGACC 
GATGGCCAGA TCCAGGCCAT GCTACGGCGC TGGTTTGTAG AAGTGGAAGG AACTGTGAAG 
GCCTACGTTT GGGACAATAA CAAGGACCTG GTGGAGTGGC TGGAGAAACA GCTGACCGAG 
GAAGATGGTG TTCGCTCAGT AATCGAGGAA AACATCAAAT ATATCAGCAG AGACTATGTC 
CTCAAGCAGA TCCGCAGCTT GGTACAGGCC AATCCCGAGG TTGCCATGGA CTCCATCGTC 
CATATGACTC AGCATATCTC ACCCACTCAG CGAGCAGAAG TCGTACGGAT CCTCTCCACG 
ATGGACTCCC CTTCAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_005624783.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624840.1 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X5, mRNA.

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CloneID	OCe12093
Clone ID Related Accession (Same CDS sequence)	XM_005624780.3 , XM_005624780.2 , XM_005624783.2 , XM_005624778.2 , XM_005624779.3 , XM_005624779.2 , XM_005624783.3 , XM_005624778.3
Accession Version	XM_005624783.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	acetyl-CoA carboxylase 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624783.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAC CATCCCCCTT GGCCAAACCT CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGTTCTG TGTCTGAAGA TAACTCAGAG GATGAAATTA GCAACCTGGT GAAGCTGGAT 
CTCCTGGAGG AGAAGGAGGG CTCTCTATCA CCAGCTTCTG TTGGCTCAGA TACACTTTCT 
GACTTGGGGA TCTCCAGCCT ACAGGACGGC TTGGCCTTAC ACATGAGGTC CAGCATGTCT 
GGCTTGCATC TAGTGAAGCA AGGTCGAGAC CGAAAGAAAA TAGACTCTCA GCGAGATTTC 
ACTGTAGCTT CTCCAGCAGA ATTTGTTACT CGTTTTGGGG GAAATAAAGT GATTGAGAAG 
GTTCTTATTG CCAACAATGG CATTGCAGCA GTGAAATGCA TGCGGTCTAT CCGTCGATGG 
TCTTATGAGA TGTTTCGAAA TGAACGTGCA ATCCGATTTG TTGTTATGGT AACACCTGAA 
GACCTGAAAG CCAATGCAGA ATACATTAAG ATGGCGGATC ACTATGTGCC AGTGCCAGGA 
GGACCAAATA ACAACAACTA TGCGAACGTG GAATTAATTC TTGATATTGC TAAAAGGATT 
CCCGTACAAG CAGTGTGGGC TGGCTGGGGT CATGCTTCTG AGAATCCCAA GCTCCCAGAA 
CTTCTTTTGA AAAATGGCAT CGCCTTCATG GGCCCTCCAA GCCAGGCCAT GTGGGCTTTG 
GGGGATAAGA TTGCATCTTC TATAGTGGCT CAAACTGCAG GTATCCCAAC TCTTCCCTGG 
AGTGGCAGTG GTCTTCGGGT GGACTGGCAT GAAAATGATT TTTCCAAACG TATCTTAAAT 
GTTCCCCAGG AATTATACGA AAAAGGTTAT GTGAAGGATG TGGATGATGG GCTAAAGGCA 
GCGGAGGAAG TTGGATATCC AGTAATGATC AAGGCCTCAG AAGGAGGAGG AGGGAAAGGA 
ATCAGAAAAG TCAACAATGC AGATGATTTC CCTAACCTCT TCAGACAGGT TCAAGCTGAA 
GTCCCTGGAT CTCCTATATT TGTGATGAGA CTAGCCAAAC AATCTCGTCA TCTGGAGGTA 
CAGATCTTAG CAGATCAATA TGGCAATGCT ATCTCTCTCT TTGGTCGTGA CTGCTCTGTA 
CAGCGCAGGC ATCAGAAGAT TATTGAGGAA GCACCTGCTG CTATTGCTAC TCCAGCAGTA 
TTTGAACATA TGGAACAGTG TGCTGTGAAA CTTGCCAAAA TGGTTGGTTA TGTGAGTGCT 
GGGACTGTGG AATACCTCTA CAGCCAGGAT GGAAGCTTCT ACTTTCTGGA ACTGAACCCT 
CGGCTACAGG TGGAACACCC TTGTACAGAG ATGGTGGCTG ATGTCAACCT CCCTGCAGCA 
CAACTCCAGA TTGCCATGGG GATCCCTCTG TACAGAATCA AGGATATTCG TATGATGTAT 
GGGGTATCTC CCTGGGGTGA TGCTCCCATT GATTTTGAAA ATTCTGCTCA CGTTCCTTGC 
CCAAGGGGCC ATGTTATTGC CGCTCGGATC ACTAGTGAAA ATCCAGATGA GGGTTTTAAG 
CCCAGCTCAG GAACAGTTCA GGAACTGAAT TTCCGTAGCA ATAAGAATGT TTGGGGTTAT 
TTCAGTGTTG CTGCTGCAGG GGGCCTTCAT GAATTTGCTG ATTCTCAGTT TGGTCATTGC 
TTCTCCTGGG GAGAAAACAG AGAGGAAGCA ATTTCAAACA TGGTGGTGGC TTTGAAGGAG 
CTGTCTATTC GGGGTGACTT TCGAACCACA GTTGAATACC TCATCAAATT GTTGGAGACT 
GAAAGCTTTC AGTTGAATAG AATTGACACC GGCTGGCTGG ACAGACTGAT AGCAGAGAAA 
GTACAGGCAG AGCGACCTGA CACCATGTTG GGAGTTGTTT GTGGGGCTCT CCATGTGGCA 
GATGTGAGCC TACGGAATAG CATCTCTAAC TTCCTTCATT CTTTAGAAAG GGGTCAAGTC 
CTTCCTGCTC ATACACTTCT GAATACAGTA GATGTTGAAC TTATCTATGG GGGAGTCAAA 
TATGTACTTA AGGTGACTCG GCAGTCCCCC AACTCCTATG TGGTGATCAT GAATGGTTCA 
TGCGTAGAAG TAGATGTGCA TCGGCTCAGT GATGGAGGGT TACTCCTGTC CTATGATGGC 
AGCAGTTACA CCACATACAT GAAGGAAGAA GTGGATAGAT ATCGTATCAC AATTGGCAAT 
AAGACCTGTG TGTTTGAGAA GGAAAATGAC CCTTCAGTGT TACGCTCACC TTCTGCTGGG 
AAGTTAATCC AGTACATTGT GGAGGATGGA GGCCATGTGT TTGCTGGCCA GTGCTATGCT 
GAAATTGAGG TGATGAAGAT GGTAATGACC TTAACAGCTG CAGAGTCTGG CTGTATCCAT 
TATGTCAAAC GGCCTGGAGC AGCTCTTGAC CCTGGCTGTG TAATAGCCAA AATGCAGTTG 
GACAACCCCA GCAAGGTCCA ACAGGCTGAG CTTCACACAG GTAGTCTGCC ACGGATCCAG 
AGCACTGCAC TCAGAGGCGA GAAGCTCCAT CGAGTGTTCC ACTATGTCCT GGATAATCTG 
GTCAACGTAA TGAATGGATA CTGCCTTCCA GATCCTTTTT TTAGCAGCAG GGTAAAAGAC 
TGGGTAGAGC GGTTGATGAA GACCCTCAGA GACCCCTCGT TACCTCTCCT AGAATTACAA 
GATATCATGA CCAGTGTCTC TGGCCGTATT CCCCCCAATG TTGAGAAGTC TATCAAGAAG 
GAAATGGCCC AGTATGCCAG CAATATCACA TCAGTCCTCT GTCAGTTTCC CAGCCAGCAG 
ATTGCCAACA TCCTAGATAG CCATGCAGCT ACGTTGAACC GGAAATCTGA GCGGGAAGTC 
TTTTTCATGA ACACTCAGAG CATTGTCCAG CTGGTACAGA GATACCGAAG TGGCATCCGA 
GGCCACATGA AGGCTGTGGT GATGGATCTG CTGCGGCAGT ACCTGCGAGT AGAGACACAG 
TTCCAGAATG GTCACTATGA CAAATGTGTG TTTGCCCTTC GAGAAGAGAA CAAGAGTGAT 
ATGAACACTG TACTGAACTA TATCTTCTCT CATGCTCAAG TCACCAAGAA GAATCTTCTG 
GTCACAATGC TTATTGATCA GTTGTGCGGT AGGGACCCTA CTCTCACTGA TGAGCTGCTG 
AATATTCTCA CAGAGCTAAC TCAACTCAGT AAGACCACCA ATGCCAAAGT AGCACTTCGA 
GCACGCCAGG TTCTTATTGC CTCCCATTTG CCATCATATG AGCTTCGCCA TAACCAAGTA 
GAGTCTATCT TCCTTTCAGC TATTGACATG TATGGACATC AGTTTTGCAT TGAGAACCTG 
CAGAAACTCA TCTTGTCTGA AACATCTATT TTTGATGTCC TACCAAACTT CTTCTATCAC 
AGCAACCAGG TAGTGAGGAT GGCAGCTCTG GAGGTTTATG TTCGAAGGGC TTATATTGCC 
TATGAACTTA ACAGCGTACA ACATCGCCAA CTTAAGGACA ACACCTGTGT GGTGGAATTC 
CAATTCATGC TACCCACATC TCATCCAAAT AGAGGGAACA TCCCCACGCT AAACAGAATG 
TCCTTCTCCT CCAACCTCAA CCACTATGGC ATGACTCATG TAGCTAGTGT CAGCGATGTG 
CTTTTGGACA ACTCATTCAC TCCACCATGT CAACGGATGG GCGGAATGGT CTCTTTTCGG 
ACTTTCGAAG ATTTTGTCAG GATCTTTGAT GAAGTGATGG GCTGCTTCTG TGATTCTCCA 
CCGCAAAGCC CTACATTCCC TGAGGCAGGT CACACATCTC TATACGATGA AGACAAGGTC 
CCCAGGGATG AACCAATTCA CATTCTGAAT GTGGCTATCA AGACTGACTG TGATATTGAG 
GATGACAGAC TGGCAGCTAT GTTCAGAGAG TTTACCCAAC AAAATAAAGC TACCCTGGTT 
GAGCATGGGA TACGGCGCCT TACTTTCCTG GTTGCACAAA AGGATTTCAG GAAACAGGTC 
AACTATGAGG TGGATCAGAG ATTTCATAGA GAATTTCCTA AATTTTTCAC CTTCCGAGCA 
AGGGATAAGT TTGAGGAGGA TCGTATCTAT CGTCACCTGG AACCTGCCCT CGCTTTCCAG 
TTAGAGCTGA ACCGGATGAG AAATTTTGAC CTTACTGCCA TTCCATGTGC TAATCATAAA 
ATGCATCTGT ATCTTGGGGC AGCCAAGGTG GAGGTGGGCA CAGAAGTGAC AGACTACAGG 
TTCTTTGTTC GTGCAATCAT CAGACATTCT GATCTGGTCA CCAAGGAAGC TTCTTTTGAA 
TACCTACAAA ATGAAGGGGA GAGGCTGCTT CTAGAAGCCA TGGATGAGTT GGAAGTTGCT 
TTTAACAATA CAAACGTTCG CACTGACTGT AACCACATCT TCCTCAACTT TGTGCCCACG 
GTCATCATGG ACCCATCAAA GATTGAGGAA TCTGTGCGGA GCATGGTAAT GCGCTATGGA 
AGTCGGCTGT GGAAATTACG TGTCCTCCAG GCAGAACTGA AAATCAACAT TCGCCTGACA 
CCAACTGGAA AAGCAATTCC CATCCGCCTC TTCCTGACAA ACGAGTCTGG CTATTACTTG 
GACATCAGCT TGTATAAGGA AGTGACTGAC TCCAGGACAG CACAGATCAT GTTTCAGGCA 
TATGGAGACA AACAGGGACC ATTGCATGGA ATGTTAATCA ACACTCCATA CGTCACCAAA 
GACCTGCTTC AATCAAAGAG ATTCCAGGCA CAATCCTTGG GGACAACATA CATATATGAT 
ATACCAGAGA TGTTTCGGCA GTCTCTGATC AAACTCTGGG AGTCTATGTC CACTCAAGCA 
TTTCTTCCAT CACCCCCCCT GCCTTCCGAC ATGCTGACAT ATACGGAGCT CGTGTTGGAT 
GATCAAGGTC AGCTGGTCCA CATGAACAGG CTTCCAGGAG GAAATGAGAT TGGCATGGTA 
GCGTGGAAAA TGACCCTTAA AAGTCCTGAA TACCCAGAAG GCCGAGATAT CATTGTTATT 
GGCAATGACA TCACATACCG AATTGGGTCC TTTGGGCCCC AGGAGGATTT GCTGTTTCTC 
AGAGCTTCTG AGCTTGCCAG GGCAGAGGGT ATCCCACGCA TCTTTGTAGC AGCCAACAGT 
GGAGCAAGAA TTGGACTGGC AGAGGAAATT CGTCATATGT TTCATGTAGC CTGGGTAGAT 
CCTGAGGATC CTTACAAGGG ATACAAATAT TTATATCTGA CCCCTCAGGA TTATAAGAGA 
GTCAGTGCTC TCAACTCTGT CCATTGTGAA CATGTAGAGG ACGAAGGAGA ATCCAGGTAC 
AAGATAACTG ATATTATTGG GAAGGAAGAG GGACTTGGAG CAGAGAACCT TCGAGGCTCT 
GGAATGATCG CTGGAGAATC CTCATTGGCC TATGATGAGA TCATCACCAT CAGCCTGGTT 
ACATGCCGGG CCATTGGGAT TGGAGCTTAC CTTGTCCGGC TGGGACAGAG AACCATCCAG 
GTTGAAAATT CTCATTTAAT TTTGACTGGA GCTGGAGCCC TCAACAAAGT CCTTGGGCGG 
GAAGTATACA CCTCCAATAA CCAGCTCGGG GGCATCCAGA TCATGCACAA CAATGGGGTG 
ACCCACAGCA TTGTTTGTGA TGACTTTGAA GGGGTTTTCA CTGTCCTGCA CTGGCTATCC 
TACATGCCTA AGAATGTGCG CAGTTTAGTT CCTCTCCTGA ACTCCAAGGA TCCTATAGAT 
AGAATCATCG AGTTTGTTCC CACGAAGGCC CCATACGATC CTCGATGGAT GCTAGCAGGC 
CGCCCTCACC CAACTCAGAA AGGTCAGTGG CTGAGTGGAT TTTTTGACTA TGGCTCTTTC 
TCAGAGATCA TGCAACCCTG GGCACAAACT GTGGTGGTTG GCAGAGCCAG GCTAGGAGGA 
ATACCTGTGG GAGTAGTTGC TGTAGAAACC CGAACAGTGG AACTAAGTAT CCCAGCCGAT 
CCTGCAAACC TGGATTCTGA AGCCAAGATA ATCCAGCAGG CTGGCCAGGT TTGGTTCCCA 
GACTCTGCGT TTAAGACCTA TCAGGCTATC AAGGACTTCA ACCGTGAAGG ACTGCCTCTG 
ATGGTCTTTG CCAACTGGAG AGGCTTCTCT GGTGGGATGA AAGATATGTA TGACCAAGTG 
CTGAAGTTTG GTGCCTATAT TGTGGATAGC TTACGGGAGT GCTCCCAGCC TGTGCTGGTA 
TACATTCCTC CTCAGGCTGA GCTCCGGGGT GGCTCCTGGG TTGTGATTGA CCCCACCATC 
AACCCCCGGC ATATGGAGAT GTACGCTGAC CGAGAAAGCA GGGGATCAGT TCTGGAGCCA 
GAAGGGACAG TAGAAATCAA ATTCCGCAGA AAGGATCTGG TGAAAACCAT GCGTCGGGTG 
GACCCAGTCT ACATTCGCTT GGCTGAGCGA TTGGGCACCC CAGAGCTCAG CGCAGCTGAG 
CGGAAGGAGC TGGAGAACAA GTTGAAGGAG CGGGAGGAAT TCCTAATTCC CATTTACCAT 
CAGGTAGCCG TGCAGTTTGC TGACTTGCAC GACACACCAG GCCGGATGCA GGAGAAGGGT 
GTTATTAATG ACATCCTGGA TTGGAAAACA TCCCGTACCT TTTTCTATTG GCGGCTGAGG 
CGTCTCCTGC TGGAGGACCT GGTCAAAAAG AAAATCCACA ATGCCAACCC AGAGCTGACC 
GATGGCCAGA TCCAGGCCAT GCTACGGCGC TGGTTTGTAG AAGTGGAAGG AACTGTGAAG 
GCCTACGTTT GGGACAATAA CAAGGACCTG GTGGAGTGGC TGGAGAAACA GCTGACCGAG 
GAAGATGGTG TTCGCTCAGT AATCGAGGAA AACATCAAAT ATATCAGCAG AGACTATGTC 
CTCAAGCAGA TCCGCAGCTT GGTACAGGCC AATCCCGAGG TTGCCATGGA CTCCATCGTC 
CATATGACTC AGCATATCTC ACCCACTCAG CGAGCAGAAG TCGTACGGAT CCTCTCCACG 
ATGGACTCCC CTTCAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624840.1
CDS	200..7240
Translation	MDEPSPLAKPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_005624783.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624783.2

ORF Insert Sequence:

ATGGATGAAC CATCCCCCTT GGCCAAACCT CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGTTCTG TGTCTGAAGA TAACTCAGAG GATGAAATTA GCAACCTGGT GAAGCTGGAT 
CTCCTGGAGG AGAAGGAGGG CTCTCTATCA CCAGCTTCTG TTGGCTCAGA TACACTTTCT 
GACTTGGGGA TCTCCAGCCT ACAGGACGGC TTGGCCTTAC ACATGAGGTC CAGCATGTCT 
GGCTTGCATC TAGTGAAGCA AGGTCGAGAC CGAAAGAAAA TAGACTCTCA GCGAGATTTC 
ACTGTAGCTT CTCCAGCAGA ATTTGTTACT CGTTTTGGGG GAAATAAAGT GATTGAGAAG 
GTTCTTATTG CCAACAATGG CATTGCAGCA GTGAAATGCA TGCGGTCTAT CCGTCGATGG 
TCTTATGAGA TGTTTCGAAA TGAACGTGCA ATCCGATTTG TTGTTATGGT AACACCTGAA 
GACCTGAAAG CCAATGCAGA ATACATTAAG ATGGCGGATC ACTATGTGCC AGTGCCAGGA 
GGACCAAATA ACAACAACTA TGCGAACGTG GAATTAATTC TTGATATTGC TAAAAGGATT 
CCCGTACAAG CAGTGTGGGC TGGCTGGGGT CATGCTTCTG AGAATCCCAA GCTCCCAGAA 
CTTCTTTTGA AAAATGGCAT CGCCTTCATG GGCCCTCCAA GCCAGGCCAT GTGGGCTTTG 
GGGGATAAGA TTGCATCTTC TATAGTGGCT CAAACTGCAG GTATCCCAAC TCTTCCCTGG 
AGTGGCAGTG GTCTTCGGGT GGACTGGCAT GAAAATGATT TTTCCAAACG TATCTTAAAT 
GTTCCCCAGG AATTATACGA AAAAGGTTAT GTGAAGGATG TGGATGATGG GCTAAAGGCA 
GCGGAGGAAG TTGGATATCC AGTAATGATC AAGGCCTCAG AAGGAGGAGG AGGGAAAGGA 
ATCAGAAAAG TCAACAATGC AGATGATTTC CCTAACCTCT TCAGACAGGT TCAAGCTGAA 
GTCCCTGGAT CTCCTATATT TGTGATGAGA CTAGCCAAAC AATCTCGTCA TCTGGAGGTA 
CAGATCTTAG CAGATCAATA TGGCAATGCT ATCTCTCTCT TTGGTCGTGA CTGCTCTGTA 
CAGCGCAGGC ATCAGAAGAT TATTGAGGAA GCACCTGCTG CTATTGCTAC TCCAGCAGTA 
TTTGAACATA TGGAACAGTG TGCTGTGAAA CTTGCCAAAA TGGTTGGTTA TGTGAGTGCT 
GGGACTGTGG AATACCTCTA CAGCCAGGAT GGAAGCTTCT ACTTTCTGGA ACTGAACCCT 
CGGCTACAGG TGGAACACCC TTGTACAGAG ATGGTGGCTG ATGTCAACCT CCCTGCAGCA 
CAACTCCAGA TTGCCATGGG GATCCCTCTG TACAGAATCA AGGATATTCG TATGATGTAT 
GGGGTATCTC CCTGGGGTGA TGCTCCCATT GATTTTGAAA ATTCTGCTCA CGTTCCTTGC 
CCAAGGGGCC ATGTTATTGC CGCTCGGATC ACTAGTGAAA ATCCAGATGA GGGTTTTAAG 
CCCAGCTCAG GAACAGTTCA GGAACTGAAT TTCCGTAGCA ATAAGAATGT TTGGGGTTAT 
TTCAGTGTTG CTGCTGCAGG GGGCCTTCAT GAATTTGCTG ATTCTCAGTT TGGTCATTGC 
TTCTCCTGGG GAGAAAACAG AGAGGAAGCA ATTTCAAACA TGGTGGTGGC TTTGAAGGAG 
CTGTCTATTC GGGGTGACTT TCGAACCACA GTTGAATACC TCATCAAATT GTTGGAGACT 
GAAAGCTTTC AGTTGAATAG AATTGACACC GGCTGGCTGG ACAGACTGAT AGCAGAGAAA 
GTACAGGCAG AGCGACCTGA CACCATGTTG GGAGTTGTTT GTGGGGCTCT CCATGTGGCA 
GATGTGAGCC TACGGAATAG CATCTCTAAC TTCCTTCATT CTTTAGAAAG GGGTCAAGTC 
CTTCCTGCTC ATACACTTCT GAATACAGTA GATGTTGAAC TTATCTATGG GGGAGTCAAA 
TATGTACTTA AGGTGACTCG GCAGTCCCCC AACTCCTATG TGGTGATCAT GAATGGTTCA 
TGCGTAGAAG TAGATGTGCA TCGGCTCAGT GATGGAGGGT TACTCCTGTC CTATGATGGC 
AGCAGTTACA CCACATACAT GAAGGAAGAA GTGGATAGAT ATCGTATCAC AATTGGCAAT 
AAGACCTGTG TGTTTGAGAA GGAAAATGAC CCTTCAGTGT TACGCTCACC TTCTGCTGGG 
AAGTTAATCC AGTACATTGT GGAGGATGGA GGCCATGTGT TTGCTGGCCA GTGCTATGCT 
GAAATTGAGG TGATGAAGAT GGTAATGACC TTAACAGCTG CAGAGTCTGG CTGTATCCAT 
TATGTCAAAC GGCCTGGAGC AGCTCTTGAC CCTGGCTGTG TAATAGCCAA AATGCAGTTG 
GACAACCCCA GCAAGGTCCA ACAGGCTGAG CTTCACACAG GTAGTCTGCC ACGGATCCAG 
AGCACTGCAC TCAGAGGCGA GAAGCTCCAT CGAGTGTTCC ACTATGTCCT GGATAATCTG 
GTCAACGTAA TGAATGGATA CTGCCTTCCA GATCCTTTTT TTAGCAGCAG GGTAAAAGAC 
TGGGTAGAGC GGTTGATGAA GACCCTCAGA GACCCCTCGT TACCTCTCCT AGAATTACAA 
GATATCATGA CCAGTGTCTC TGGCCGTATT CCCCCCAATG TTGAGAAGTC TATCAAGAAG 
GAAATGGCCC AGTATGCCAG CAATATCACA TCAGTCCTCT GTCAGTTTCC CAGCCAGCAG 
ATTGCCAACA TCCTAGATAG CCATGCAGCT ACGTTGAACC GGAAATCTGA GCGGGAAGTC 
TTTTTCATGA ACACTCAGAG CATTGTCCAG CTGGTACAGA GATACCGAAG TGGCATCCGA 
GGCCACATGA AGGCTGTGGT GATGGATCTG CTGCGGCAGT ACCTGCGAGT AGAGACACAG 
TTCCAGAATG GTCACTATGA CAAATGTGTG TTTGCCCTTC GAGAAGAGAA CAAGAGTGAT 
ATGAACACTG TACTGAACTA TATCTTCTCT CATGCTCAAG TCACCAAGAA GAATCTTCTG 
GTCACAATGC TTATTGATCA GTTGTGCGGT AGGGACCCTA CTCTCACTGA TGAGCTGCTG 
AATATTCTCA CAGAGCTAAC TCAACTCAGT AAGACCACCA ATGCCAAAGT AGCACTTCGA 
GCACGCCAGG TTCTTATTGC CTCCCATTTG CCATCATATG AGCTTCGCCA TAACCAAGTA 
GAGTCTATCT TCCTTTCAGC TATTGACATG TATGGACATC AGTTTTGCAT TGAGAACCTG 
CAGAAACTCA TCTTGTCTGA AACATCTATT TTTGATGTCC TACCAAACTT CTTCTATCAC 
AGCAACCAGG TAGTGAGGAT GGCAGCTCTG GAGGTTTATG TTCGAAGGGC TTATATTGCC 
TATGAACTTA ACAGCGTACA ACATCGCCAA CTTAAGGACA ACACCTGTGT GGTGGAATTC 
CAATTCATGC TACCCACATC TCATCCAAAT AGAGGGAACA TCCCCACGCT AAACAGAATG 
TCCTTCTCCT CCAACCTCAA CCACTATGGC ATGACTCATG TAGCTAGTGT CAGCGATGTG 
CTTTTGGACA ACTCATTCAC TCCACCATGT CAACGGATGG GCGGAATGGT CTCTTTTCGG 
ACTTTCGAAG ATTTTGTCAG GATCTTTGAT GAAGTGATGG GCTGCTTCTG TGATTCTCCA 
CCGCAAAGCC CTACATTCCC TGAGGCAGGT CACACATCTC TATACGATGA AGACAAGGTC 
CCCAGGGATG AACCAATTCA CATTCTGAAT GTGGCTATCA AGACTGACTG TGATATTGAG 
GATGACAGAC TGGCAGCTAT GTTCAGAGAG TTTACCCAAC AAAATAAAGC TACCCTGGTT 
GAGCATGGGA TACGGCGCCT TACTTTCCTG GTTGCACAAA AGGATTTCAG GAAACAGGTC 
AACTATGAGG TGGATCAGAG ATTTCATAGA GAATTTCCTA AATTTTTCAC CTTCCGAGCA 
AGGGATAAGT TTGAGGAGGA TCGTATCTAT CGTCACCTGG AACCTGCCCT CGCTTTCCAG 
TTAGAGCTGA ACCGGATGAG AAATTTTGAC CTTACTGCCA TTCCATGTGC TAATCATAAA 
ATGCATCTGT ATCTTGGGGC AGCCAAGGTG GAGGTGGGCA CAGAAGTGAC AGACTACAGG 
TTCTTTGTTC GTGCAATCAT CAGACATTCT GATCTGGTCA CCAAGGAAGC TTCTTTTGAA 
TACCTACAAA ATGAAGGGGA GAGGCTGCTT CTAGAAGCCA TGGATGAGTT GGAAGTTGCT 
TTTAACAATA CAAACGTTCG CACTGACTGT AACCACATCT TCCTCAACTT TGTGCCCACG 
GTCATCATGG ACCCATCAAA GATTGAGGAA TCTGTGCGGA GCATGGTAAT GCGCTATGGA 
AGTCGGCTGT GGAAATTACG TGTCCTCCAG GCAGAACTGA AAATCAACAT TCGCCTGACA 
CCAACTGGAA AAGCAATTCC CATCCGCCTC TTCCTGACAA ACGAGTCTGG CTATTACTTG 
GACATCAGCT TGTATAAGGA AGTGACTGAC TCCAGGACAG CACAGATCAT GTTTCAGGCA 
TATGGAGACA AACAGGGACC ATTGCATGGA ATGTTAATCA ACACTCCATA CGTCACCAAA 
GACCTGCTTC AATCAAAGAG ATTCCAGGCA CAATCCTTGG GGACAACATA CATATATGAT 
ATACCAGAGA TGTTTCGGCA GTCTCTGATC AAACTCTGGG AGTCTATGTC CACTCAAGCA 
TTTCTTCCAT CACCCCCCCT GCCTTCCGAC ATGCTGACAT ATACGGAGCT CGTGTTGGAT 
GATCAAGGTC AGCTGGTCCA CATGAACAGG CTTCCAGGAG GAAATGAGAT TGGCATGGTA 
GCGTGGAAAA TGACCCTTAA AAGTCCTGAA TACCCAGAAG GCCGAGATAT CATTGTTATT 
GGCAATGACA TCACATACCG AATTGGGTCC TTTGGGCCCC AGGAGGATTT GCTGTTTCTC 
AGAGCTTCTG AGCTTGCCAG GGCAGAGGGT ATCCCACGCA TCTTTGTAGC AGCCAACAGT 
GGAGCAAGAA TTGGACTGGC AGAGGAAATT CGTCATATGT TTCATGTAGC CTGGGTAGAT 
CCTGAGGATC CTTACAAGGG ATACAAATAT TTATATCTGA CCCCTCAGGA TTATAAGAGA 
GTCAGTGCTC TCAACTCTGT CCATTGTGAA CATGTAGAGG ACGAAGGAGA ATCCAGGTAC 
AAGATAACTG ATATTATTGG GAAGGAAGAG GGACTTGGAG CAGAGAACCT TCGAGGCTCT 
GGAATGATCG CTGGAGAATC CTCATTGGCC TATGATGAGA TCATCACCAT CAGCCTGGTT 
ACATGCCGGG CCATTGGGAT TGGAGCTTAC CTTGTCCGGC TGGGACAGAG AACCATCCAG 
GTTGAAAATT CTCATTTAAT TTTGACTGGA GCTGGAGCCC TCAACAAAGT CCTTGGGCGG 
GAAGTATACA CCTCCAATAA CCAGCTCGGG GGCATCCAGA TCATGCACAA CAATGGGGTG 
ACCCACAGCA TTGTTTGTGA TGACTTTGAA GGGGTTTTCA CTGTCCTGCA CTGGCTATCC 
TACATGCCTA AGAATGTGCG CAGTTTAGTT CCTCTCCTGA ACTCCAAGGA TCCTATAGAT 
AGAATCATCG AGTTTGTTCC CACGAAGGCC CCATACGATC CTCGATGGAT GCTAGCAGGC 
CGCCCTCACC CAACTCAGAA AGGTCAGTGG CTGAGTGGAT TTTTTGACTA TGGCTCTTTC 
TCAGAGATCA TGCAACCCTG GGCACAAACT GTGGTGGTTG GCAGAGCCAG GCTAGGAGGA 
ATACCTGTGG GAGTAGTTGC TGTAGAAACC CGAACAGTGG AACTAAGTAT CCCAGCCGAT 
CCTGCAAACC TGGATTCTGA AGCCAAGATA ATCCAGCAGG CTGGCCAGGT TTGGTTCCCA 
GACTCTGCGT TTAAGACCTA TCAGGCTATC AAGGACTTCA ACCGTGAAGG ACTGCCTCTG 
ATGGTCTTTG CCAACTGGAG AGGCTTCTCT GGTGGGATGA AAGATATGTA TGACCAAGTG 
CTGAAGTTTG GTGCCTATAT TGTGGATAGC TTACGGGAGT GCTCCCAGCC TGTGCTGGTA 
TACATTCCTC CTCAGGCTGA GCTCCGGGGT GGCTCCTGGG TTGTGATTGA CCCCACCATC 
AACCCCCGGC ATATGGAGAT GTACGCTGAC CGAGAAAGCA GGGGATCAGT TCTGGAGCCA 
GAAGGGACAG TAGAAATCAA ATTCCGCAGA AAGGATCTGG TGAAAACCAT GCGTCGGGTG 
GACCCAGTCT ACATTCGCTT GGCTGAGCGA TTGGGCACCC CAGAGCTCAG CGCAGCTGAG 
CGGAAGGAGC TGGAGAACAA GTTGAAGGAG CGGGAGGAAT TCCTAATTCC CATTTACCAT 
CAGGTAGCCG TGCAGTTTGC TGACTTGCAC GACACACCAG GCCGGATGCA GGAGAAGGGT 
GTTATTAATG ACATCCTGGA TTGGAAAACA TCCCGTACCT TTTTCTATTG GCGGCTGAGG 
CGTCTCCTGC TGGAGGACCT GGTCAAAAAG AAAATCCACA ATGCCAACCC AGAGCTGACC 
GATGGCCAGA TCCAGGCCAT GCTACGGCGC TGGTTTGTAG AAGTGGAAGG AACTGTGAAG 
GCCTACGTTT GGGACAATAA CAAGGACCTG GTGGAGTGGC TGGAGAAACA GCTGACCGAG 
GAAGATGGTG TTCGCTCAGT AATCGAGGAA AACATCAAAT ATATCAGCAG AGACTATGTC 
CTCAAGCAGA TCCGCAGCTT GGTACAGGCC AATCCCGAGG TTGCCATGGA CTCCATCGTC 
CATATGACTC AGCATATCTC ACCCACTCAG CGAGCAGAAG TCGTACGGAT CCTCTCCACG 
ATGGACTCCC CTTCAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_005624778.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624835.1 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X6, mRNA.

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CloneID	OCe12093
Clone ID Related Accession (Same CDS sequence)	XM_005624780.3 , XM_005624780.2 , XM_005624783.2 , XM_005624778.2 , XM_005624779.3 , XM_005624779.2 , XM_005624783.3 , XM_005624778.3
Accession Version	XM_005624778.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	acetyl-CoA carboxylase 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAC CATCCCCCTT GGCCAAACCT CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGTTCTG TGTCTGAAGA TAACTCAGAG GATGAAATTA GCAACCTGGT GAAGCTGGAT 
CTCCTGGAGG AGAAGGAGGG CTCTCTATCA CCAGCTTCTG TTGGCTCAGA TACACTTTCT 
GACTTGGGGA TCTCCAGCCT ACAGGACGGC TTGGCCTTAC ACATGAGGTC CAGCATGTCT 
GGCTTGCATC TAGTGAAGCA AGGTCGAGAC CGAAAGAAAA TAGACTCTCA GCGAGATTTC 
ACTGTAGCTT CTCCAGCAGA ATTTGTTACT CGTTTTGGGG GAAATAAAGT GATTGAGAAG 
GTTCTTATTG CCAACAATGG CATTGCAGCA GTGAAATGCA TGCGGTCTAT CCGTCGATGG 
TCTTATGAGA TGTTTCGAAA TGAACGTGCA ATCCGATTTG TTGTTATGGT AACACCTGAA 
GACCTGAAAG CCAATGCAGA ATACATTAAG ATGGCGGATC ACTATGTGCC AGTGCCAGGA 
GGACCAAATA ACAACAACTA TGCGAACGTG GAATTAATTC TTGATATTGC TAAAAGGATT 
CCCGTACAAG CAGTGTGGGC TGGCTGGGGT CATGCTTCTG AGAATCCCAA GCTCCCAGAA 
CTTCTTTTGA AAAATGGCAT CGCCTTCATG GGCCCTCCAA GCCAGGCCAT GTGGGCTTTG 
GGGGATAAGA TTGCATCTTC TATAGTGGCT CAAACTGCAG GTATCCCAAC TCTTCCCTGG 
AGTGGCAGTG GTCTTCGGGT GGACTGGCAT GAAAATGATT TTTCCAAACG TATCTTAAAT 
GTTCCCCAGG AATTATACGA AAAAGGTTAT GTGAAGGATG TGGATGATGG GCTAAAGGCA 
GCGGAGGAAG TTGGATATCC AGTAATGATC AAGGCCTCAG AAGGAGGAGG AGGGAAAGGA 
ATCAGAAAAG TCAACAATGC AGATGATTTC CCTAACCTCT TCAGACAGGT TCAAGCTGAA 
GTCCCTGGAT CTCCTATATT TGTGATGAGA CTAGCCAAAC AATCTCGTCA TCTGGAGGTA 
CAGATCTTAG CAGATCAATA TGGCAATGCT ATCTCTCTCT TTGGTCGTGA CTGCTCTGTA 
CAGCGCAGGC ATCAGAAGAT TATTGAGGAA GCACCTGCTG CTATTGCTAC TCCAGCAGTA 
TTTGAACATA TGGAACAGTG TGCTGTGAAA CTTGCCAAAA TGGTTGGTTA TGTGAGTGCT 
GGGACTGTGG AATACCTCTA CAGCCAGGAT GGAAGCTTCT ACTTTCTGGA ACTGAACCCT 
CGGCTACAGG TGGAACACCC TTGTACAGAG ATGGTGGCTG ATGTCAACCT CCCTGCAGCA 
CAACTCCAGA TTGCCATGGG GATCCCTCTG TACAGAATCA AGGATATTCG TATGATGTAT 
GGGGTATCTC CCTGGGGTGA TGCTCCCATT GATTTTGAAA ATTCTGCTCA CGTTCCTTGC 
CCAAGGGGCC ATGTTATTGC CGCTCGGATC ACTAGTGAAA ATCCAGATGA GGGTTTTAAG 
CCCAGCTCAG GAACAGTTCA GGAACTGAAT TTCCGTAGCA ATAAGAATGT TTGGGGTTAT 
TTCAGTGTTG CTGCTGCAGG GGGCCTTCAT GAATTTGCTG ATTCTCAGTT TGGTCATTGC 
TTCTCCTGGG GAGAAAACAG AGAGGAAGCA ATTTCAAACA TGGTGGTGGC TTTGAAGGAG 
CTGTCTATTC GGGGTGACTT TCGAACCACA GTTGAATACC TCATCAAATT GTTGGAGACT 
GAAAGCTTTC AGTTGAATAG AATTGACACC GGCTGGCTGG ACAGACTGAT AGCAGAGAAA 
GTACAGGCAG AGCGACCTGA CACCATGTTG GGAGTTGTTT GTGGGGCTCT CCATGTGGCA 
GATGTGAGCC TACGGAATAG CATCTCTAAC TTCCTTCATT CTTTAGAAAG GGGTCAAGTC 
CTTCCTGCTC ATACACTTCT GAATACAGTA GATGTTGAAC TTATCTATGG GGGAGTCAAA 
TATGTACTTA AGGTGACTCG GCAGTCCCCC AACTCCTATG TGGTGATCAT GAATGGTTCA 
TGCGTAGAAG TAGATGTGCA TCGGCTCAGT GATGGAGGGT TACTCCTGTC CTATGATGGC 
AGCAGTTACA CCACATACAT GAAGGAAGAA GTGGATAGAT ATCGTATCAC AATTGGCAAT 
AAGACCTGTG TGTTTGAGAA GGAAAATGAC CCTTCAGTGT TACGCTCACC TTCTGCTGGG 
AAGTTAATCC AGTACATTGT GGAGGATGGA GGCCATGTGT TTGCTGGCCA GTGCTATGCT 
GAAATTGAGG TGATGAAGAT GGTAATGACC TTAACAGCTG CAGAGTCTGG CTGTATCCAT 
TATGTCAAAC GGCCTGGAGC AGCTCTTGAC CCTGGCTGTG TAATAGCCAA AATGCAGTTG 
GACAACCCCA GCAAGGTCCA ACAGGCTGAG CTTCACACAG GTAGTCTGCC ACGGATCCAG 
AGCACTGCAC TCAGAGGCGA GAAGCTCCAT CGAGTGTTCC ACTATGTCCT GGATAATCTG 
GTCAACGTAA TGAATGGATA CTGCCTTCCA GATCCTTTTT TTAGCAGCAG GGTAAAAGAC 
TGGGTAGAGC GGTTGATGAA GACCCTCAGA GACCCCTCGT TACCTCTCCT AGAATTACAA 
GATATCATGA CCAGTGTCTC TGGCCGTATT CCCCCCAATG TTGAGAAGTC TATCAAGAAG 
GAAATGGCCC AGTATGCCAG CAATATCACA TCAGTCCTCT GTCAGTTTCC CAGCCAGCAG 
ATTGCCAACA TCCTAGATAG CCATGCAGCT ACGTTGAACC GGAAATCTGA GCGGGAAGTC 
TTTTTCATGA ACACTCAGAG CATTGTCCAG CTGGTACAGA GATACCGAAG TGGCATCCGA 
GGCCACATGA AGGCTGTGGT GATGGATCTG CTGCGGCAGT ACCTGCGAGT AGAGACACAG 
TTCCAGAATG GTCACTATGA CAAATGTGTG TTTGCCCTTC GAGAAGAGAA CAAGAGTGAT 
ATGAACACTG TACTGAACTA TATCTTCTCT CATGCTCAAG TCACCAAGAA GAATCTTCTG 
GTCACAATGC TTATTGATCA GTTGTGCGGT AGGGACCCTA CTCTCACTGA TGAGCTGCTG 
AATATTCTCA CAGAGCTAAC TCAACTCAGT AAGACCACCA ATGCCAAAGT AGCACTTCGA 
GCACGCCAGG TTCTTATTGC CTCCCATTTG CCATCATATG AGCTTCGCCA TAACCAAGTA 
GAGTCTATCT TCCTTTCAGC TATTGACATG TATGGACATC AGTTTTGCAT TGAGAACCTG 
CAGAAACTCA TCTTGTCTGA AACATCTATT TTTGATGTCC TACCAAACTT CTTCTATCAC 
AGCAACCAGG TAGTGAGGAT GGCAGCTCTG GAGGTTTATG TTCGAAGGGC TTATATTGCC 
TATGAACTTA ACAGCGTACA ACATCGCCAA CTTAAGGACA ACACCTGTGT GGTGGAATTC 
CAATTCATGC TACCCACATC TCATCCAAAT AGAGGGAACA TCCCCACGCT AAACAGAATG 
TCCTTCTCCT CCAACCTCAA CCACTATGGC ATGACTCATG TAGCTAGTGT CAGCGATGTG 
CTTTTGGACA ACTCATTCAC TCCACCATGT CAACGGATGG GCGGAATGGT CTCTTTTCGG 
ACTTTCGAAG ATTTTGTCAG GATCTTTGAT GAAGTGATGG GCTGCTTCTG TGATTCTCCA 
CCGCAAAGCC CTACATTCCC TGAGGCAGGT CACACATCTC TATACGATGA AGACAAGGTC 
CCCAGGGATG AACCAATTCA CATTCTGAAT GTGGCTATCA AGACTGACTG TGATATTGAG 
GATGACAGAC TGGCAGCTAT GTTCAGAGAG TTTACCCAAC AAAATAAAGC TACCCTGGTT 
GAGCATGGGA TACGGCGCCT TACTTTCCTG GTTGCACAAA AGGATTTCAG GAAACAGGTC 
AACTATGAGG TGGATCAGAG ATTTCATAGA GAATTTCCTA AATTTTTCAC CTTCCGAGCA 
AGGGATAAGT TTGAGGAGGA TCGTATCTAT CGTCACCTGG AACCTGCCCT CGCTTTCCAG 
TTAGAGCTGA ACCGGATGAG AAATTTTGAC CTTACTGCCA TTCCATGTGC TAATCATAAA 
ATGCATCTGT ATCTTGGGGC AGCCAAGGTG GAGGTGGGCA CAGAAGTGAC AGACTACAGG 
TTCTTTGTTC GTGCAATCAT CAGACATTCT GATCTGGTCA CCAAGGAAGC TTCTTTTGAA 
TACCTACAAA ATGAAGGGGA GAGGCTGCTT CTAGAAGCCA TGGATGAGTT GGAAGTTGCT 
TTTAACAATA CAAACGTTCG CACTGACTGT AACCACATCT TCCTCAACTT TGTGCCCACG 
GTCATCATGG ACCCATCAAA GATTGAGGAA TCTGTGCGGA GCATGGTAAT GCGCTATGGA 
AGTCGGCTGT GGAAATTACG TGTCCTCCAG GCAGAACTGA AAATCAACAT TCGCCTGACA 
CCAACTGGAA AAGCAATTCC CATCCGCCTC TTCCTGACAA ACGAGTCTGG CTATTACTTG 
GACATCAGCT TGTATAAGGA AGTGACTGAC TCCAGGACAG CACAGATCAT GTTTCAGGCA 
TATGGAGACA AACAGGGACC ATTGCATGGA ATGTTAATCA ACACTCCATA CGTCACCAAA 
GACCTGCTTC AATCAAAGAG ATTCCAGGCA CAATCCTTGG GGACAACATA CATATATGAT 
ATACCAGAGA TGTTTCGGCA GTCTCTGATC AAACTCTGGG AGTCTATGTC CACTCAAGCA 
TTTCTTCCAT CACCCCCCCT GCCTTCCGAC ATGCTGACAT ATACGGAGCT CGTGTTGGAT 
GATCAAGGTC AGCTGGTCCA CATGAACAGG CTTCCAGGAG GAAATGAGAT TGGCATGGTA 
GCGTGGAAAA TGACCCTTAA AAGTCCTGAA TACCCAGAAG GCCGAGATAT CATTGTTATT 
GGCAATGACA TCACATACCG AATTGGGTCC TTTGGGCCCC AGGAGGATTT GCTGTTTCTC 
AGAGCTTCTG AGCTTGCCAG GGCAGAGGGT ATCCCACGCA TCTTTGTAGC AGCCAACAGT 
GGAGCAAGAA TTGGACTGGC AGAGGAAATT CGTCATATGT TTCATGTAGC CTGGGTAGAT 
CCTGAGGATC CTTACAAGGG ATACAAATAT TTATATCTGA CCCCTCAGGA TTATAAGAGA 
GTCAGTGCTC TCAACTCTGT CCATTGTGAA CATGTAGAGG ACGAAGGAGA ATCCAGGTAC 
AAGATAACTG ATATTATTGG GAAGGAAGAG GGACTTGGAG CAGAGAACCT TCGAGGCTCT 
GGAATGATCG CTGGAGAATC CTCATTGGCC TATGATGAGA TCATCACCAT CAGCCTGGTT 
ACATGCCGGG CCATTGGGAT TGGAGCTTAC CTTGTCCGGC TGGGACAGAG AACCATCCAG 
GTTGAAAATT CTCATTTAAT TTTGACTGGA GCTGGAGCCC TCAACAAAGT CCTTGGGCGG 
GAAGTATACA CCTCCAATAA CCAGCTCGGG GGCATCCAGA TCATGCACAA CAATGGGGTG 
ACCCACAGCA TTGTTTGTGA TGACTTTGAA GGGGTTTTCA CTGTCCTGCA CTGGCTATCC 
TACATGCCTA AGAATGTGCG CAGTTTAGTT CCTCTCCTGA ACTCCAAGGA TCCTATAGAT 
AGAATCATCG AGTTTGTTCC CACGAAGGCC CCATACGATC CTCGATGGAT GCTAGCAGGC 
CGCCCTCACC CAACTCAGAA AGGTCAGTGG CTGAGTGGAT TTTTTGACTA TGGCTCTTTC 
TCAGAGATCA TGCAACCCTG GGCACAAACT GTGGTGGTTG GCAGAGCCAG GCTAGGAGGA 
ATACCTGTGG GAGTAGTTGC TGTAGAAACC CGAACAGTGG AACTAAGTAT CCCAGCCGAT 
CCTGCAAACC TGGATTCTGA AGCCAAGATA ATCCAGCAGG CTGGCCAGGT TTGGTTCCCA 
GACTCTGCGT TTAAGACCTA TCAGGCTATC AAGGACTTCA ACCGTGAAGG ACTGCCTCTG 
ATGGTCTTTG CCAACTGGAG AGGCTTCTCT GGTGGGATGA AAGATATGTA TGACCAAGTG 
CTGAAGTTTG GTGCCTATAT TGTGGATAGC TTACGGGAGT GCTCCCAGCC TGTGCTGGTA 
TACATTCCTC CTCAGGCTGA GCTCCGGGGT GGCTCCTGGG TTGTGATTGA CCCCACCATC 
AACCCCCGGC ATATGGAGAT GTACGCTGAC CGAGAAAGCA GGGGATCAGT TCTGGAGCCA 
GAAGGGACAG TAGAAATCAA ATTCCGCAGA AAGGATCTGG TGAAAACCAT GCGTCGGGTG 
GACCCAGTCT ACATTCGCTT GGCTGAGCGA TTGGGCACCC CAGAGCTCAG CGCAGCTGAG 
CGGAAGGAGC TGGAGAACAA GTTGAAGGAG CGGGAGGAAT TCCTAATTCC CATTTACCAT 
CAGGTAGCCG TGCAGTTTGC TGACTTGCAC GACACACCAG GCCGGATGCA GGAGAAGGGT 
GTTATTAATG ACATCCTGGA TTGGAAAACA TCCCGTACCT TTTTCTATTG GCGGCTGAGG 
CGTCTCCTGC TGGAGGACCT GGTCAAAAAG AAAATCCACA ATGCCAACCC AGAGCTGACC 
GATGGCCAGA TCCAGGCCAT GCTACGGCGC TGGTTTGTAG AAGTGGAAGG AACTGTGAAG 
GCCTACGTTT GGGACAATAA CAAGGACCTG GTGGAGTGGC TGGAGAAACA GCTGACCGAG 
GAAGATGGTG TTCGCTCAGT AATCGAGGAA AACATCAAAT ATATCAGCAG AGACTATGTC 
CTCAAGCAGA TCCGCAGCTT GGTACAGGCC AATCCCGAGG TTGCCATGGA CTCCATCGTC 
CATATGACTC AGCATATCTC ACCCACTCAG CGAGCAGAAG TCGTACGGAT CCTCTCCACG 
ATGGACTCCC CTTCAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624835.1
CDS	153..7193
Translation	MDEPSPLAKPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_005624778.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624778.2

ORF Insert Sequence:

ATGGATGAAC CATCCCCCTT GGCCAAACCT CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGTTCTG TGTCTGAAGA TAACTCAGAG GATGAAATTA GCAACCTGGT GAAGCTGGAT 
CTCCTGGAGG AGAAGGAGGG CTCTCTATCA CCAGCTTCTG TTGGCTCAGA TACACTTTCT 
GACTTGGGGA TCTCCAGCCT ACAGGACGGC TTGGCCTTAC ACATGAGGTC CAGCATGTCT 
GGCTTGCATC TAGTGAAGCA AGGTCGAGAC CGAAAGAAAA TAGACTCTCA GCGAGATTTC 
ACTGTAGCTT CTCCAGCAGA ATTTGTTACT CGTTTTGGGG GAAATAAAGT GATTGAGAAG 
GTTCTTATTG CCAACAATGG CATTGCAGCA GTGAAATGCA TGCGGTCTAT CCGTCGATGG 
TCTTATGAGA TGTTTCGAAA TGAACGTGCA ATCCGATTTG TTGTTATGGT AACACCTGAA 
GACCTGAAAG CCAATGCAGA ATACATTAAG ATGGCGGATC ACTATGTGCC AGTGCCAGGA 
GGACCAAATA ACAACAACTA TGCGAACGTG GAATTAATTC TTGATATTGC TAAAAGGATT 
CCCGTACAAG CAGTGTGGGC TGGCTGGGGT CATGCTTCTG AGAATCCCAA GCTCCCAGAA 
CTTCTTTTGA AAAATGGCAT CGCCTTCATG GGCCCTCCAA GCCAGGCCAT GTGGGCTTTG 
GGGGATAAGA TTGCATCTTC TATAGTGGCT CAAACTGCAG GTATCCCAAC TCTTCCCTGG 
AGTGGCAGTG GTCTTCGGGT GGACTGGCAT GAAAATGATT TTTCCAAACG TATCTTAAAT 
GTTCCCCAGG AATTATACGA AAAAGGTTAT GTGAAGGATG TGGATGATGG GCTAAAGGCA 
GCGGAGGAAG TTGGATATCC AGTAATGATC AAGGCCTCAG AAGGAGGAGG AGGGAAAGGA 
ATCAGAAAAG TCAACAATGC AGATGATTTC CCTAACCTCT TCAGACAGGT TCAAGCTGAA 
GTCCCTGGAT CTCCTATATT TGTGATGAGA CTAGCCAAAC AATCTCGTCA TCTGGAGGTA 
CAGATCTTAG CAGATCAATA TGGCAATGCT ATCTCTCTCT TTGGTCGTGA CTGCTCTGTA 
CAGCGCAGGC ATCAGAAGAT TATTGAGGAA GCACCTGCTG CTATTGCTAC TCCAGCAGTA 
TTTGAACATA TGGAACAGTG TGCTGTGAAA CTTGCCAAAA TGGTTGGTTA TGTGAGTGCT 
GGGACTGTGG AATACCTCTA CAGCCAGGAT GGAAGCTTCT ACTTTCTGGA ACTGAACCCT 
CGGCTACAGG TGGAACACCC TTGTACAGAG ATGGTGGCTG ATGTCAACCT CCCTGCAGCA 
CAACTCCAGA TTGCCATGGG GATCCCTCTG TACAGAATCA AGGATATTCG TATGATGTAT 
GGGGTATCTC CCTGGGGTGA TGCTCCCATT GATTTTGAAA ATTCTGCTCA CGTTCCTTGC 
CCAAGGGGCC ATGTTATTGC CGCTCGGATC ACTAGTGAAA ATCCAGATGA GGGTTTTAAG 
CCCAGCTCAG GAACAGTTCA GGAACTGAAT TTCCGTAGCA ATAAGAATGT TTGGGGTTAT 
TTCAGTGTTG CTGCTGCAGG GGGCCTTCAT GAATTTGCTG ATTCTCAGTT TGGTCATTGC 
TTCTCCTGGG GAGAAAACAG AGAGGAAGCA ATTTCAAACA TGGTGGTGGC TTTGAAGGAG 
CTGTCTATTC GGGGTGACTT TCGAACCACA GTTGAATACC TCATCAAATT GTTGGAGACT 
GAAAGCTTTC AGTTGAATAG AATTGACACC GGCTGGCTGG ACAGACTGAT AGCAGAGAAA 
GTACAGGCAG AGCGACCTGA CACCATGTTG GGAGTTGTTT GTGGGGCTCT CCATGTGGCA 
GATGTGAGCC TACGGAATAG CATCTCTAAC TTCCTTCATT CTTTAGAAAG GGGTCAAGTC 
CTTCCTGCTC ATACACTTCT GAATACAGTA GATGTTGAAC TTATCTATGG GGGAGTCAAA 
TATGTACTTA AGGTGACTCG GCAGTCCCCC AACTCCTATG TGGTGATCAT GAATGGTTCA 
TGCGTAGAAG TAGATGTGCA TCGGCTCAGT GATGGAGGGT TACTCCTGTC CTATGATGGC 
AGCAGTTACA CCACATACAT GAAGGAAGAA GTGGATAGAT ATCGTATCAC AATTGGCAAT 
AAGACCTGTG TGTTTGAGAA GGAAAATGAC CCTTCAGTGT TACGCTCACC TTCTGCTGGG 
AAGTTAATCC AGTACATTGT GGAGGATGGA GGCCATGTGT TTGCTGGCCA GTGCTATGCT 
GAAATTGAGG TGATGAAGAT GGTAATGACC TTAACAGCTG CAGAGTCTGG CTGTATCCAT 
TATGTCAAAC GGCCTGGAGC AGCTCTTGAC CCTGGCTGTG TAATAGCCAA AATGCAGTTG 
GACAACCCCA GCAAGGTCCA ACAGGCTGAG CTTCACACAG GTAGTCTGCC ACGGATCCAG 
AGCACTGCAC TCAGAGGCGA GAAGCTCCAT CGAGTGTTCC ACTATGTCCT GGATAATCTG 
GTCAACGTAA TGAATGGATA CTGCCTTCCA GATCCTTTTT TTAGCAGCAG GGTAAAAGAC 
TGGGTAGAGC GGTTGATGAA GACCCTCAGA GACCCCTCGT TACCTCTCCT AGAATTACAA 
GATATCATGA CCAGTGTCTC TGGCCGTATT CCCCCCAATG TTGAGAAGTC TATCAAGAAG 
GAAATGGCCC AGTATGCCAG CAATATCACA TCAGTCCTCT GTCAGTTTCC CAGCCAGCAG 
ATTGCCAACA TCCTAGATAG CCATGCAGCT ACGTTGAACC GGAAATCTGA GCGGGAAGTC 
TTTTTCATGA ACACTCAGAG CATTGTCCAG CTGGTACAGA GATACCGAAG TGGCATCCGA 
GGCCACATGA AGGCTGTGGT GATGGATCTG CTGCGGCAGT ACCTGCGAGT AGAGACACAG 
TTCCAGAATG GTCACTATGA CAAATGTGTG TTTGCCCTTC GAGAAGAGAA CAAGAGTGAT 
ATGAACACTG TACTGAACTA TATCTTCTCT CATGCTCAAG TCACCAAGAA GAATCTTCTG 
GTCACAATGC TTATTGATCA GTTGTGCGGT AGGGACCCTA CTCTCACTGA TGAGCTGCTG 
AATATTCTCA CAGAGCTAAC TCAACTCAGT AAGACCACCA ATGCCAAAGT AGCACTTCGA 
GCACGCCAGG TTCTTATTGC CTCCCATTTG CCATCATATG AGCTTCGCCA TAACCAAGTA 
GAGTCTATCT TCCTTTCAGC TATTGACATG TATGGACATC AGTTTTGCAT TGAGAACCTG 
CAGAAACTCA TCTTGTCTGA AACATCTATT TTTGATGTCC TACCAAACTT CTTCTATCAC 
AGCAACCAGG TAGTGAGGAT GGCAGCTCTG GAGGTTTATG TTCGAAGGGC TTATATTGCC 
TATGAACTTA ACAGCGTACA ACATCGCCAA CTTAAGGACA ACACCTGTGT GGTGGAATTC 
CAATTCATGC TACCCACATC TCATCCAAAT AGAGGGAACA TCCCCACGCT AAACAGAATG 
TCCTTCTCCT CCAACCTCAA CCACTATGGC ATGACTCATG TAGCTAGTGT CAGCGATGTG 
CTTTTGGACA ACTCATTCAC TCCACCATGT CAACGGATGG GCGGAATGGT CTCTTTTCGG 
ACTTTCGAAG ATTTTGTCAG GATCTTTGAT GAAGTGATGG GCTGCTTCTG TGATTCTCCA 
CCGCAAAGCC CTACATTCCC TGAGGCAGGT CACACATCTC TATACGATGA AGACAAGGTC 
CCCAGGGATG AACCAATTCA CATTCTGAAT GTGGCTATCA AGACTGACTG TGATATTGAG 
GATGACAGAC TGGCAGCTAT GTTCAGAGAG TTTACCCAAC AAAATAAAGC TACCCTGGTT 
GAGCATGGGA TACGGCGCCT TACTTTCCTG GTTGCACAAA AGGATTTCAG GAAACAGGTC 
AACTATGAGG TGGATCAGAG ATTTCATAGA GAATTTCCTA AATTTTTCAC CTTCCGAGCA 
AGGGATAAGT TTGAGGAGGA TCGTATCTAT CGTCACCTGG AACCTGCCCT CGCTTTCCAG 
TTAGAGCTGA ACCGGATGAG AAATTTTGAC CTTACTGCCA TTCCATGTGC TAATCATAAA 
ATGCATCTGT ATCTTGGGGC AGCCAAGGTG GAGGTGGGCA CAGAAGTGAC AGACTACAGG 
TTCTTTGTTC GTGCAATCAT CAGACATTCT GATCTGGTCA CCAAGGAAGC TTCTTTTGAA 
TACCTACAAA ATGAAGGGGA GAGGCTGCTT CTAGAAGCCA TGGATGAGTT GGAAGTTGCT 
TTTAACAATA CAAACGTTCG CACTGACTGT AACCACATCT TCCTCAACTT TGTGCCCACG 
GTCATCATGG ACCCATCAAA GATTGAGGAA TCTGTGCGGA GCATGGTAAT GCGCTATGGA 
AGTCGGCTGT GGAAATTACG TGTCCTCCAG GCAGAACTGA AAATCAACAT TCGCCTGACA 
CCAACTGGAA AAGCAATTCC CATCCGCCTC TTCCTGACAA ACGAGTCTGG CTATTACTTG 
GACATCAGCT TGTATAAGGA AGTGACTGAC TCCAGGACAG CACAGATCAT GTTTCAGGCA 
TATGGAGACA AACAGGGACC ATTGCATGGA ATGTTAATCA ACACTCCATA CGTCACCAAA 
GACCTGCTTC AATCAAAGAG ATTCCAGGCA CAATCCTTGG GGACAACATA CATATATGAT 
ATACCAGAGA TGTTTCGGCA GTCTCTGATC AAACTCTGGG AGTCTATGTC CACTCAAGCA 
TTTCTTCCAT CACCCCCCCT GCCTTCCGAC ATGCTGACAT ATACGGAGCT CGTGTTGGAT 
GATCAAGGTC AGCTGGTCCA CATGAACAGG CTTCCAGGAG GAAATGAGAT TGGCATGGTA 
GCGTGGAAAA TGACCCTTAA AAGTCCTGAA TACCCAGAAG GCCGAGATAT CATTGTTATT 
GGCAATGACA TCACATACCG AATTGGGTCC TTTGGGCCCC AGGAGGATTT GCTGTTTCTC 
AGAGCTTCTG AGCTTGCCAG GGCAGAGGGT ATCCCACGCA TCTTTGTAGC AGCCAACAGT 
GGAGCAAGAA TTGGACTGGC AGAGGAAATT CGTCATATGT TTCATGTAGC CTGGGTAGAT 
CCTGAGGATC CTTACAAGGG ATACAAATAT TTATATCTGA CCCCTCAGGA TTATAAGAGA 
GTCAGTGCTC TCAACTCTGT CCATTGTGAA CATGTAGAGG ACGAAGGAGA ATCCAGGTAC 
AAGATAACTG ATATTATTGG GAAGGAAGAG GGACTTGGAG CAGAGAACCT TCGAGGCTCT 
GGAATGATCG CTGGAGAATC CTCATTGGCC TATGATGAGA TCATCACCAT CAGCCTGGTT 
ACATGCCGGG CCATTGGGAT TGGAGCTTAC CTTGTCCGGC TGGGACAGAG AACCATCCAG 
GTTGAAAATT CTCATTTAAT TTTGACTGGA GCTGGAGCCC TCAACAAAGT CCTTGGGCGG 
GAAGTATACA CCTCCAATAA CCAGCTCGGG GGCATCCAGA TCATGCACAA CAATGGGGTG 
ACCCACAGCA TTGTTTGTGA TGACTTTGAA GGGGTTTTCA CTGTCCTGCA CTGGCTATCC 
TACATGCCTA AGAATGTGCG CAGTTTAGTT CCTCTCCTGA ACTCCAAGGA TCCTATAGAT 
AGAATCATCG AGTTTGTTCC CACGAAGGCC CCATACGATC CTCGATGGAT GCTAGCAGGC 
CGCCCTCACC CAACTCAGAA AGGTCAGTGG CTGAGTGGAT TTTTTGACTA TGGCTCTTTC 
TCAGAGATCA TGCAACCCTG GGCACAAACT GTGGTGGTTG GCAGAGCCAG GCTAGGAGGA 
ATACCTGTGG GAGTAGTTGC TGTAGAAACC CGAACAGTGG AACTAAGTAT CCCAGCCGAT 
CCTGCAAACC TGGATTCTGA AGCCAAGATA ATCCAGCAGG CTGGCCAGGT TTGGTTCCCA 
GACTCTGCGT TTAAGACCTA TCAGGCTATC AAGGACTTCA ACCGTGAAGG ACTGCCTCTG 
ATGGTCTTTG CCAACTGGAG AGGCTTCTCT GGTGGGATGA AAGATATGTA TGACCAAGTG 
CTGAAGTTTG GTGCCTATAT TGTGGATAGC TTACGGGAGT GCTCCCAGCC TGTGCTGGTA 
TACATTCCTC CTCAGGCTGA GCTCCGGGGT GGCTCCTGGG TTGTGATTGA CCCCACCATC 
AACCCCCGGC ATATGGAGAT GTACGCTGAC CGAGAAAGCA GGGGATCAGT TCTGGAGCCA 
GAAGGGACAG TAGAAATCAA ATTCCGCAGA AAGGATCTGG TGAAAACCAT GCGTCGGGTG 
GACCCAGTCT ACATTCGCTT GGCTGAGCGA TTGGGCACCC CAGAGCTCAG CGCAGCTGAG 
CGGAAGGAGC TGGAGAACAA GTTGAAGGAG CGGGAGGAAT TCCTAATTCC CATTTACCAT 
CAGGTAGCCG TGCAGTTTGC TGACTTGCAC GACACACCAG GCCGGATGCA GGAGAAGGGT 
GTTATTAATG ACATCCTGGA TTGGAAAACA TCCCGTACCT TTTTCTATTG GCGGCTGAGG 
CGTCTCCTGC TGGAGGACCT GGTCAAAAAG AAAATCCACA ATGCCAACCC AGAGCTGACC 
GATGGCCAGA TCCAGGCCAT GCTACGGCGC TGGTTTGTAG AAGTGGAAGG AACTGTGAAG 
GCCTACGTTT GGGACAATAA CAAGGACCTG GTGGAGTGGC TGGAGAAACA GCTGACCGAG 
GAAGATGGTG TTCGCTCAGT AATCGAGGAA AACATCAAAT ATATCAGCAG AGACTATGTC 
CTCAAGCAGA TCCGCAGCTT GGTACAGGCC AATCCCGAGG TTGCCATGGA CTCCATCGTC 
CATATGACTC AGCATATCTC ACCCACTCAG CGAGCAGAAG TCGTACGGAT CCTCTCCACG 
ATGGACTCCC CTTCAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_005624779.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624836.1 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X4, mRNA.

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CloneID	OCe12093
Clone ID Related Accession (Same CDS sequence)	XM_005624780.3 , XM_005624780.2 , XM_005624783.2 , XM_005624778.2 , XM_005624779.3 , XM_005624779.2 , XM_005624783.3 , XM_005624778.3
Accession Version	XM_005624779.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	acetyl-CoA carboxylase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624779.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAC CATCCCCCTT GGCCAAACCT CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGTTCTG TGTCTGAAGA TAACTCAGAG GATGAAATTA GCAACCTGGT GAAGCTGGAT 
CTCCTGGAGG AGAAGGAGGG CTCTCTATCA CCAGCTTCTG TTGGCTCAGA TACACTTTCT 
GACTTGGGGA TCTCCAGCCT ACAGGACGGC TTGGCCTTAC ACATGAGGTC CAGCATGTCT 
GGCTTGCATC TAGTGAAGCA AGGTCGAGAC CGAAAGAAAA TAGACTCTCA GCGAGATTTC 
ACTGTAGCTT CTCCAGCAGA ATTTGTTACT CGTTTTGGGG GAAATAAAGT GATTGAGAAG 
GTTCTTATTG CCAACAATGG CATTGCAGCA GTGAAATGCA TGCGGTCTAT CCGTCGATGG 
TCTTATGAGA TGTTTCGAAA TGAACGTGCA ATCCGATTTG TTGTTATGGT AACACCTGAA 
GACCTGAAAG CCAATGCAGA ATACATTAAG ATGGCGGATC ACTATGTGCC AGTGCCAGGA 
GGACCAAATA ACAACAACTA TGCGAACGTG GAATTAATTC TTGATATTGC TAAAAGGATT 
CCCGTACAAG CAGTGTGGGC TGGCTGGGGT CATGCTTCTG AGAATCCCAA GCTCCCAGAA 
CTTCTTTTGA AAAATGGCAT CGCCTTCATG GGCCCTCCAA GCCAGGCCAT GTGGGCTTTG 
GGGGATAAGA TTGCATCTTC TATAGTGGCT CAAACTGCAG GTATCCCAAC TCTTCCCTGG 
AGTGGCAGTG GTCTTCGGGT GGACTGGCAT GAAAATGATT TTTCCAAACG TATCTTAAAT 
GTTCCCCAGG AATTATACGA AAAAGGTTAT GTGAAGGATG TGGATGATGG GCTAAAGGCA 
GCGGAGGAAG TTGGATATCC AGTAATGATC AAGGCCTCAG AAGGAGGAGG AGGGAAAGGA 
ATCAGAAAAG TCAACAATGC AGATGATTTC CCTAACCTCT TCAGACAGGT TCAAGCTGAA 
GTCCCTGGAT CTCCTATATT TGTGATGAGA CTAGCCAAAC AATCTCGTCA TCTGGAGGTA 
CAGATCTTAG CAGATCAATA TGGCAATGCT ATCTCTCTCT TTGGTCGTGA CTGCTCTGTA 
CAGCGCAGGC ATCAGAAGAT TATTGAGGAA GCACCTGCTG CTATTGCTAC TCCAGCAGTA 
TTTGAACATA TGGAACAGTG TGCTGTGAAA CTTGCCAAAA TGGTTGGTTA TGTGAGTGCT 
GGGACTGTGG AATACCTCTA CAGCCAGGAT GGAAGCTTCT ACTTTCTGGA ACTGAACCCT 
CGGCTACAGG TGGAACACCC TTGTACAGAG ATGGTGGCTG ATGTCAACCT CCCTGCAGCA 
CAACTCCAGA TTGCCATGGG GATCCCTCTG TACAGAATCA AGGATATTCG TATGATGTAT 
GGGGTATCTC CCTGGGGTGA TGCTCCCATT GATTTTGAAA ATTCTGCTCA CGTTCCTTGC 
CCAAGGGGCC ATGTTATTGC CGCTCGGATC ACTAGTGAAA ATCCAGATGA GGGTTTTAAG 
CCCAGCTCAG GAACAGTTCA GGAACTGAAT TTCCGTAGCA ATAAGAATGT TTGGGGTTAT 
TTCAGTGTTG CTGCTGCAGG GGGCCTTCAT GAATTTGCTG ATTCTCAGTT TGGTCATTGC 
TTCTCCTGGG GAGAAAACAG AGAGGAAGCA ATTTCAAACA TGGTGGTGGC TTTGAAGGAG 
CTGTCTATTC GGGGTGACTT TCGAACCACA GTTGAATACC TCATCAAATT GTTGGAGACT 
GAAAGCTTTC AGTTGAATAG AATTGACACC GGCTGGCTGG ACAGACTGAT AGCAGAGAAA 
GTACAGGCAG AGCGACCTGA CACCATGTTG GGAGTTGTTT GTGGGGCTCT CCATGTGGCA 
GATGTGAGCC TACGGAATAG CATCTCTAAC TTCCTTCATT CTTTAGAAAG GGGTCAAGTC 
CTTCCTGCTC ATACACTTCT GAATACAGTA GATGTTGAAC TTATCTATGG GGGAGTCAAA 
TATGTACTTA AGGTGACTCG GCAGTCCCCC AACTCCTATG TGGTGATCAT GAATGGTTCA 
TGCGTAGAAG TAGATGTGCA TCGGCTCAGT GATGGAGGGT TACTCCTGTC CTATGATGGC 
AGCAGTTACA CCACATACAT GAAGGAAGAA GTGGATAGAT ATCGTATCAC AATTGGCAAT 
AAGACCTGTG TGTTTGAGAA GGAAAATGAC CCTTCAGTGT TACGCTCACC TTCTGCTGGG 
AAGTTAATCC AGTACATTGT GGAGGATGGA GGCCATGTGT TTGCTGGCCA GTGCTATGCT 
GAAATTGAGG TGATGAAGAT GGTAATGACC TTAACAGCTG CAGAGTCTGG CTGTATCCAT 
TATGTCAAAC GGCCTGGAGC AGCTCTTGAC CCTGGCTGTG TAATAGCCAA AATGCAGTTG 
GACAACCCCA GCAAGGTCCA ACAGGCTGAG CTTCACACAG GTAGTCTGCC ACGGATCCAG 
AGCACTGCAC TCAGAGGCGA GAAGCTCCAT CGAGTGTTCC ACTATGTCCT GGATAATCTG 
GTCAACGTAA TGAATGGATA CTGCCTTCCA GATCCTTTTT TTAGCAGCAG GGTAAAAGAC 
TGGGTAGAGC GGTTGATGAA GACCCTCAGA GACCCCTCGT TACCTCTCCT AGAATTACAA 
GATATCATGA CCAGTGTCTC TGGCCGTATT CCCCCCAATG TTGAGAAGTC TATCAAGAAG 
GAAATGGCCC AGTATGCCAG CAATATCACA TCAGTCCTCT GTCAGTTTCC CAGCCAGCAG 
ATTGCCAACA TCCTAGATAG CCATGCAGCT ACGTTGAACC GGAAATCTGA GCGGGAAGTC 
TTTTTCATGA ACACTCAGAG CATTGTCCAG CTGGTACAGA GATACCGAAG TGGCATCCGA 
GGCCACATGA AGGCTGTGGT GATGGATCTG CTGCGGCAGT ACCTGCGAGT AGAGACACAG 
TTCCAGAATG GTCACTATGA CAAATGTGTG TTTGCCCTTC GAGAAGAGAA CAAGAGTGAT 
ATGAACACTG TACTGAACTA TATCTTCTCT CATGCTCAAG TCACCAAGAA GAATCTTCTG 
GTCACAATGC TTATTGATCA GTTGTGCGGT AGGGACCCTA CTCTCACTGA TGAGCTGCTG 
AATATTCTCA CAGAGCTAAC TCAACTCAGT AAGACCACCA ATGCCAAAGT AGCACTTCGA 
GCACGCCAGG TTCTTATTGC CTCCCATTTG CCATCATATG AGCTTCGCCA TAACCAAGTA 
GAGTCTATCT TCCTTTCAGC TATTGACATG TATGGACATC AGTTTTGCAT TGAGAACCTG 
CAGAAACTCA TCTTGTCTGA AACATCTATT TTTGATGTCC TACCAAACTT CTTCTATCAC 
AGCAACCAGG TAGTGAGGAT GGCAGCTCTG GAGGTTTATG TTCGAAGGGC TTATATTGCC 
TATGAACTTA ACAGCGTACA ACATCGCCAA CTTAAGGACA ACACCTGTGT GGTGGAATTC 
CAATTCATGC TACCCACATC TCATCCAAAT AGAGGGAACA TCCCCACGCT AAACAGAATG 
TCCTTCTCCT CCAACCTCAA CCACTATGGC ATGACTCATG TAGCTAGTGT CAGCGATGTG 
CTTTTGGACA ACTCATTCAC TCCACCATGT CAACGGATGG GCGGAATGGT CTCTTTTCGG 
ACTTTCGAAG ATTTTGTCAG GATCTTTGAT GAAGTGATGG GCTGCTTCTG TGATTCTCCA 
CCGCAAAGCC CTACATTCCC TGAGGCAGGT CACACATCTC TATACGATGA AGACAAGGTC 
CCCAGGGATG AACCAATTCA CATTCTGAAT GTGGCTATCA AGACTGACTG TGATATTGAG 
GATGACAGAC TGGCAGCTAT GTTCAGAGAG TTTACCCAAC AAAATAAAGC TACCCTGGTT 
GAGCATGGGA TACGGCGCCT TACTTTCCTG GTTGCACAAA AGGATTTCAG GAAACAGGTC 
AACTATGAGG TGGATCAGAG ATTTCATAGA GAATTTCCTA AATTTTTCAC CTTCCGAGCA 
AGGGATAAGT TTGAGGAGGA TCGTATCTAT CGTCACCTGG AACCTGCCCT CGCTTTCCAG 
TTAGAGCTGA ACCGGATGAG AAATTTTGAC CTTACTGCCA TTCCATGTGC TAATCATAAA 
ATGCATCTGT ATCTTGGGGC AGCCAAGGTG GAGGTGGGCA CAGAAGTGAC AGACTACAGG 
TTCTTTGTTC GTGCAATCAT CAGACATTCT GATCTGGTCA CCAAGGAAGC TTCTTTTGAA 
TACCTACAAA ATGAAGGGGA GAGGCTGCTT CTAGAAGCCA TGGATGAGTT GGAAGTTGCT 
TTTAACAATA CAAACGTTCG CACTGACTGT AACCACATCT TCCTCAACTT TGTGCCCACG 
GTCATCATGG ACCCATCAAA GATTGAGGAA TCTGTGCGGA GCATGGTAAT GCGCTATGGA 
AGTCGGCTGT GGAAATTACG TGTCCTCCAG GCAGAACTGA AAATCAACAT TCGCCTGACA 
CCAACTGGAA AAGCAATTCC CATCCGCCTC TTCCTGACAA ACGAGTCTGG CTATTACTTG 
GACATCAGCT TGTATAAGGA AGTGACTGAC TCCAGGACAG CACAGATCAT GTTTCAGGCA 
TATGGAGACA AACAGGGACC ATTGCATGGA ATGTTAATCA ACACTCCATA CGTCACCAAA 
GACCTGCTTC AATCAAAGAG ATTCCAGGCA CAATCCTTGG GGACAACATA CATATATGAT 
ATACCAGAGA TGTTTCGGCA GTCTCTGATC AAACTCTGGG AGTCTATGTC CACTCAAGCA 
TTTCTTCCAT CACCCCCCCT GCCTTCCGAC ATGCTGACAT ATACGGAGCT CGTGTTGGAT 
GATCAAGGTC AGCTGGTCCA CATGAACAGG CTTCCAGGAG GAAATGAGAT TGGCATGGTA 
GCGTGGAAAA TGACCCTTAA AAGTCCTGAA TACCCAGAAG GCCGAGATAT CATTGTTATT 
GGCAATGACA TCACATACCG AATTGGGTCC TTTGGGCCCC AGGAGGATTT GCTGTTTCTC 
AGAGCTTCTG AGCTTGCCAG GGCAGAGGGT ATCCCACGCA TCTTTGTAGC AGCCAACAGT 
GGAGCAAGAA TTGGACTGGC AGAGGAAATT CGTCATATGT TTCATGTAGC CTGGGTAGAT 
CCTGAGGATC CTTACAAGGG ATACAAATAT TTATATCTGA CCCCTCAGGA TTATAAGAGA 
GTCAGTGCTC TCAACTCTGT CCATTGTGAA CATGTAGAGG ACGAAGGAGA ATCCAGGTAC 
AAGATAACTG ATATTATTGG GAAGGAAGAG GGACTTGGAG CAGAGAACCT TCGAGGCTCT 
GGAATGATCG CTGGAGAATC CTCATTGGCC TATGATGAGA TCATCACCAT CAGCCTGGTT 
ACATGCCGGG CCATTGGGAT TGGAGCTTAC CTTGTCCGGC TGGGACAGAG AACCATCCAG 
GTTGAAAATT CTCATTTAAT TTTGACTGGA GCTGGAGCCC TCAACAAAGT CCTTGGGCGG 
GAAGTATACA CCTCCAATAA CCAGCTCGGG GGCATCCAGA TCATGCACAA CAATGGGGTG 
ACCCACAGCA TTGTTTGTGA TGACTTTGAA GGGGTTTTCA CTGTCCTGCA CTGGCTATCC 
TACATGCCTA AGAATGTGCG CAGTTTAGTT CCTCTCCTGA ACTCCAAGGA TCCTATAGAT 
AGAATCATCG AGTTTGTTCC CACGAAGGCC CCATACGATC CTCGATGGAT GCTAGCAGGC 
CGCCCTCACC CAACTCAGAA AGGTCAGTGG CTGAGTGGAT TTTTTGACTA TGGCTCTTTC 
TCAGAGATCA TGCAACCCTG GGCACAAACT GTGGTGGTTG GCAGAGCCAG GCTAGGAGGA 
ATACCTGTGG GAGTAGTTGC TGTAGAAACC CGAACAGTGG AACTAAGTAT CCCAGCCGAT 
CCTGCAAACC TGGATTCTGA AGCCAAGATA ATCCAGCAGG CTGGCCAGGT TTGGTTCCCA 
GACTCTGCGT TTAAGACCTA TCAGGCTATC AAGGACTTCA ACCGTGAAGG ACTGCCTCTG 
ATGGTCTTTG CCAACTGGAG AGGCTTCTCT GGTGGGATGA AAGATATGTA TGACCAAGTG 
CTGAAGTTTG GTGCCTATAT TGTGGATAGC TTACGGGAGT GCTCCCAGCC TGTGCTGGTA 
TACATTCCTC CTCAGGCTGA GCTCCGGGGT GGCTCCTGGG TTGTGATTGA CCCCACCATC 
AACCCCCGGC ATATGGAGAT GTACGCTGAC CGAGAAAGCA GGGGATCAGT TCTGGAGCCA 
GAAGGGACAG TAGAAATCAA ATTCCGCAGA AAGGATCTGG TGAAAACCAT GCGTCGGGTG 
GACCCAGTCT ACATTCGCTT GGCTGAGCGA TTGGGCACCC CAGAGCTCAG CGCAGCTGAG 
CGGAAGGAGC TGGAGAACAA GTTGAAGGAG CGGGAGGAAT TCCTAATTCC CATTTACCAT 
CAGGTAGCCG TGCAGTTTGC TGACTTGCAC GACACACCAG GCCGGATGCA GGAGAAGGGT 
GTTATTAATG ACATCCTGGA TTGGAAAACA TCCCGTACCT TTTTCTATTG GCGGCTGAGG 
CGTCTCCTGC TGGAGGACCT GGTCAAAAAG AAAATCCACA ATGCCAACCC AGAGCTGACC 
GATGGCCAGA TCCAGGCCAT GCTACGGCGC TGGTTTGTAG AAGTGGAAGG AACTGTGAAG 
GCCTACGTTT GGGACAATAA CAAGGACCTG GTGGAGTGGC TGGAGAAACA GCTGACCGAG 
GAAGATGGTG TTCGCTCAGT AATCGAGGAA AACATCAAAT ATATCAGCAG AGACTATGTC 
CTCAAGCAGA TCCGCAGCTT GGTACAGGCC AATCCCGAGG TTGCCATGGA CTCCATCGTC 
CATATGACTC AGCATATCTC ACCCACTCAG CGAGCAGAAG TCGTACGGAT CCTCTCCACG 
ATGGACTCCC CTTCAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624836.1
CDS	323..7363
Translation	MDEPSPLAKPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_005624779.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624779.3

ORF Insert Sequence:

ATGGATGAAC CATCCCCCTT GGCCAAACCT CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGTTCTG TGTCTGAAGA TAACTCAGAG GATGAAATTA GCAACCTGGT GAAGCTGGAT 
CTCCTGGAGG AGAAGGAGGG CTCTCTATCA CCAGCTTCTG TTGGCTCAGA TACACTTTCT 
GACTTGGGGA TCTCCAGCCT ACAGGACGGC TTGGCCTTAC ACATGAGGTC CAGCATGTCT 
GGCTTGCATC TAGTGAAGCA AGGTCGAGAC CGAAAGAAAA TAGACTCTCA GCGAGATTTC 
ACTGTAGCTT CTCCAGCAGA ATTTGTTACT CGTTTTGGGG GAAATAAAGT GATTGAGAAG 
GTTCTTATTG CCAACAATGG CATTGCAGCA GTGAAATGCA TGCGGTCTAT CCGTCGATGG 
TCTTATGAGA TGTTTCGAAA TGAACGTGCA ATCCGATTTG TTGTTATGGT AACACCTGAA 
GACCTGAAAG CCAATGCAGA ATACATTAAG ATGGCGGATC ACTATGTGCC AGTGCCAGGA 
GGACCAAATA ACAACAACTA TGCGAACGTG GAATTAATTC TTGATATTGC TAAAAGGATT 
CCCGTACAAG CAGTGTGGGC TGGCTGGGGT CATGCTTCTG AGAATCCCAA GCTCCCAGAA 
CTTCTTTTGA AAAATGGCAT CGCCTTCATG GGCCCTCCAA GCCAGGCCAT GTGGGCTTTG 
GGGGATAAGA TTGCATCTTC TATAGTGGCT CAAACTGCAG GTATCCCAAC TCTTCCCTGG 
AGTGGCAGTG GTCTTCGGGT GGACTGGCAT GAAAATGATT TTTCCAAACG TATCTTAAAT 
GTTCCCCAGG AATTATACGA AAAAGGTTAT GTGAAGGATG TGGATGATGG GCTAAAGGCA 
GCGGAGGAAG TTGGATATCC AGTAATGATC AAGGCCTCAG AAGGAGGAGG AGGGAAAGGA 
ATCAGAAAAG TCAACAATGC AGATGATTTC CCTAACCTCT TCAGACAGGT TCAAGCTGAA 
GTCCCTGGAT CTCCTATATT TGTGATGAGA CTAGCCAAAC AATCTCGTCA TCTGGAGGTA 
CAGATCTTAG CAGATCAATA TGGCAATGCT ATCTCTCTCT TTGGTCGTGA CTGCTCTGTA 
CAGCGCAGGC ATCAGAAGAT TATTGAGGAA GCACCTGCTG CTATTGCTAC TCCAGCAGTA 
TTTGAACATA TGGAACAGTG TGCTGTGAAA CTTGCCAAAA TGGTTGGTTA TGTGAGTGCT 
GGGACTGTGG AATACCTCTA CAGCCAGGAT GGAAGCTTCT ACTTTCTGGA ACTGAACCCT 
CGGCTACAGG TGGAACACCC TTGTACAGAG ATGGTGGCTG ATGTCAACCT CCCTGCAGCA 
CAACTCCAGA TTGCCATGGG GATCCCTCTG TACAGAATCA AGGATATTCG TATGATGTAT 
GGGGTATCTC CCTGGGGTGA TGCTCCCATT GATTTTGAAA ATTCTGCTCA CGTTCCTTGC 
CCAAGGGGCC ATGTTATTGC CGCTCGGATC ACTAGTGAAA ATCCAGATGA GGGTTTTAAG 
CCCAGCTCAG GAACAGTTCA GGAACTGAAT TTCCGTAGCA ATAAGAATGT TTGGGGTTAT 
TTCAGTGTTG CTGCTGCAGG GGGCCTTCAT GAATTTGCTG ATTCTCAGTT TGGTCATTGC 
TTCTCCTGGG GAGAAAACAG AGAGGAAGCA ATTTCAAACA TGGTGGTGGC TTTGAAGGAG 
CTGTCTATTC GGGGTGACTT TCGAACCACA GTTGAATACC TCATCAAATT GTTGGAGACT 
GAAAGCTTTC AGTTGAATAG AATTGACACC GGCTGGCTGG ACAGACTGAT AGCAGAGAAA 
GTACAGGCAG AGCGACCTGA CACCATGTTG GGAGTTGTTT GTGGGGCTCT CCATGTGGCA 
GATGTGAGCC TACGGAATAG CATCTCTAAC TTCCTTCATT CTTTAGAAAG GGGTCAAGTC 
CTTCCTGCTC ATACACTTCT GAATACAGTA GATGTTGAAC TTATCTATGG GGGAGTCAAA 
TATGTACTTA AGGTGACTCG GCAGTCCCCC AACTCCTATG TGGTGATCAT GAATGGTTCA 
TGCGTAGAAG TAGATGTGCA TCGGCTCAGT GATGGAGGGT TACTCCTGTC CTATGATGGC 
AGCAGTTACA CCACATACAT GAAGGAAGAA GTGGATAGAT ATCGTATCAC AATTGGCAAT 
AAGACCTGTG TGTTTGAGAA GGAAAATGAC CCTTCAGTGT TACGCTCACC TTCTGCTGGG 
AAGTTAATCC AGTACATTGT GGAGGATGGA GGCCATGTGT TTGCTGGCCA GTGCTATGCT 
GAAATTGAGG TGATGAAGAT GGTAATGACC TTAACAGCTG CAGAGTCTGG CTGTATCCAT 
TATGTCAAAC GGCCTGGAGC AGCTCTTGAC CCTGGCTGTG TAATAGCCAA AATGCAGTTG 
GACAACCCCA GCAAGGTCCA ACAGGCTGAG CTTCACACAG GTAGTCTGCC ACGGATCCAG 
AGCACTGCAC TCAGAGGCGA GAAGCTCCAT CGAGTGTTCC ACTATGTCCT GGATAATCTG 
GTCAACGTAA TGAATGGATA CTGCCTTCCA GATCCTTTTT TTAGCAGCAG GGTAAAAGAC 
TGGGTAGAGC GGTTGATGAA GACCCTCAGA GACCCCTCGT TACCTCTCCT AGAATTACAA 
GATATCATGA CCAGTGTCTC TGGCCGTATT CCCCCCAATG TTGAGAAGTC TATCAAGAAG 
GAAATGGCCC AGTATGCCAG CAATATCACA TCAGTCCTCT GTCAGTTTCC CAGCCAGCAG 
ATTGCCAACA TCCTAGATAG CCATGCAGCT ACGTTGAACC GGAAATCTGA GCGGGAAGTC 
TTTTTCATGA ACACTCAGAG CATTGTCCAG CTGGTACAGA GATACCGAAG TGGCATCCGA 
GGCCACATGA AGGCTGTGGT GATGGATCTG CTGCGGCAGT ACCTGCGAGT AGAGACACAG 
TTCCAGAATG GTCACTATGA CAAATGTGTG TTTGCCCTTC GAGAAGAGAA CAAGAGTGAT 
ATGAACACTG TACTGAACTA TATCTTCTCT CATGCTCAAG TCACCAAGAA GAATCTTCTG 
GTCACAATGC TTATTGATCA GTTGTGCGGT AGGGACCCTA CTCTCACTGA TGAGCTGCTG 
AATATTCTCA CAGAGCTAAC TCAACTCAGT AAGACCACCA ATGCCAAAGT AGCACTTCGA 
GCACGCCAGG TTCTTATTGC CTCCCATTTG CCATCATATG AGCTTCGCCA TAACCAAGTA 
GAGTCTATCT TCCTTTCAGC TATTGACATG TATGGACATC AGTTTTGCAT TGAGAACCTG 
CAGAAACTCA TCTTGTCTGA AACATCTATT TTTGATGTCC TACCAAACTT CTTCTATCAC 
AGCAACCAGG TAGTGAGGAT GGCAGCTCTG GAGGTTTATG TTCGAAGGGC TTATATTGCC 
TATGAACTTA ACAGCGTACA ACATCGCCAA CTTAAGGACA ACACCTGTGT GGTGGAATTC 
CAATTCATGC TACCCACATC TCATCCAAAT AGAGGGAACA TCCCCACGCT AAACAGAATG 
TCCTTCTCCT CCAACCTCAA CCACTATGGC ATGACTCATG TAGCTAGTGT CAGCGATGTG 
CTTTTGGACA ACTCATTCAC TCCACCATGT CAACGGATGG GCGGAATGGT CTCTTTTCGG 
ACTTTCGAAG ATTTTGTCAG GATCTTTGAT GAAGTGATGG GCTGCTTCTG TGATTCTCCA 
CCGCAAAGCC CTACATTCCC TGAGGCAGGT CACACATCTC TATACGATGA AGACAAGGTC 
CCCAGGGATG AACCAATTCA CATTCTGAAT GTGGCTATCA AGACTGACTG TGATATTGAG 
GATGACAGAC TGGCAGCTAT GTTCAGAGAG TTTACCCAAC AAAATAAAGC TACCCTGGTT 
GAGCATGGGA TACGGCGCCT TACTTTCCTG GTTGCACAAA AGGATTTCAG GAAACAGGTC 
AACTATGAGG TGGATCAGAG ATTTCATAGA GAATTTCCTA AATTTTTCAC CTTCCGAGCA 
AGGGATAAGT TTGAGGAGGA TCGTATCTAT CGTCACCTGG AACCTGCCCT CGCTTTCCAG 
TTAGAGCTGA ACCGGATGAG AAATTTTGAC CTTACTGCCA TTCCATGTGC TAATCATAAA 
ATGCATCTGT ATCTTGGGGC AGCCAAGGTG GAGGTGGGCA CAGAAGTGAC AGACTACAGG 
TTCTTTGTTC GTGCAATCAT CAGACATTCT GATCTGGTCA CCAAGGAAGC TTCTTTTGAA 
TACCTACAAA ATGAAGGGGA GAGGCTGCTT CTAGAAGCCA TGGATGAGTT GGAAGTTGCT 
TTTAACAATA CAAACGTTCG CACTGACTGT AACCACATCT TCCTCAACTT TGTGCCCACG 
GTCATCATGG ACCCATCAAA GATTGAGGAA TCTGTGCGGA GCATGGTAAT GCGCTATGGA 
AGTCGGCTGT GGAAATTACG TGTCCTCCAG GCAGAACTGA AAATCAACAT TCGCCTGACA 
CCAACTGGAA AAGCAATTCC CATCCGCCTC TTCCTGACAA ACGAGTCTGG CTATTACTTG 
GACATCAGCT TGTATAAGGA AGTGACTGAC TCCAGGACAG CACAGATCAT GTTTCAGGCA 
TATGGAGACA AACAGGGACC ATTGCATGGA ATGTTAATCA ACACTCCATA CGTCACCAAA 
GACCTGCTTC AATCAAAGAG ATTCCAGGCA CAATCCTTGG GGACAACATA CATATATGAT 
ATACCAGAGA TGTTTCGGCA GTCTCTGATC AAACTCTGGG AGTCTATGTC CACTCAAGCA 
TTTCTTCCAT CACCCCCCCT GCCTTCCGAC ATGCTGACAT ATACGGAGCT CGTGTTGGAT 
GATCAAGGTC AGCTGGTCCA CATGAACAGG CTTCCAGGAG GAAATGAGAT TGGCATGGTA 
GCGTGGAAAA TGACCCTTAA AAGTCCTGAA TACCCAGAAG GCCGAGATAT CATTGTTATT 
GGCAATGACA TCACATACCG AATTGGGTCC TTTGGGCCCC AGGAGGATTT GCTGTTTCTC 
AGAGCTTCTG AGCTTGCCAG GGCAGAGGGT ATCCCACGCA TCTTTGTAGC AGCCAACAGT 
GGAGCAAGAA TTGGACTGGC AGAGGAAATT CGTCATATGT TTCATGTAGC CTGGGTAGAT 
CCTGAGGATC CTTACAAGGG ATACAAATAT TTATATCTGA CCCCTCAGGA TTATAAGAGA 
GTCAGTGCTC TCAACTCTGT CCATTGTGAA CATGTAGAGG ACGAAGGAGA ATCCAGGTAC 
AAGATAACTG ATATTATTGG GAAGGAAGAG GGACTTGGAG CAGAGAACCT TCGAGGCTCT 
GGAATGATCG CTGGAGAATC CTCATTGGCC TATGATGAGA TCATCACCAT CAGCCTGGTT 
ACATGCCGGG CCATTGGGAT TGGAGCTTAC CTTGTCCGGC TGGGACAGAG AACCATCCAG 
GTTGAAAATT CTCATTTAAT TTTGACTGGA GCTGGAGCCC TCAACAAAGT CCTTGGGCGG 
GAAGTATACA CCTCCAATAA CCAGCTCGGG GGCATCCAGA TCATGCACAA CAATGGGGTG 
ACCCACAGCA TTGTTTGTGA TGACTTTGAA GGGGTTTTCA CTGTCCTGCA CTGGCTATCC 
TACATGCCTA AGAATGTGCG CAGTTTAGTT CCTCTCCTGA ACTCCAAGGA TCCTATAGAT 
AGAATCATCG AGTTTGTTCC CACGAAGGCC CCATACGATC CTCGATGGAT GCTAGCAGGC 
CGCCCTCACC CAACTCAGAA AGGTCAGTGG CTGAGTGGAT TTTTTGACTA TGGCTCTTTC 
TCAGAGATCA TGCAACCCTG GGCACAAACT GTGGTGGTTG GCAGAGCCAG GCTAGGAGGA 
ATACCTGTGG GAGTAGTTGC TGTAGAAACC CGAACAGTGG AACTAAGTAT CCCAGCCGAT 
CCTGCAAACC TGGATTCTGA AGCCAAGATA ATCCAGCAGG CTGGCCAGGT TTGGTTCCCA 
GACTCTGCGT TTAAGACCTA TCAGGCTATC AAGGACTTCA ACCGTGAAGG ACTGCCTCTG 
ATGGTCTTTG CCAACTGGAG AGGCTTCTCT GGTGGGATGA AAGATATGTA TGACCAAGTG 
CTGAAGTTTG GTGCCTATAT TGTGGATAGC TTACGGGAGT GCTCCCAGCC TGTGCTGGTA 
TACATTCCTC CTCAGGCTGA GCTCCGGGGT GGCTCCTGGG TTGTGATTGA CCCCACCATC 
AACCCCCGGC ATATGGAGAT GTACGCTGAC CGAGAAAGCA GGGGATCAGT TCTGGAGCCA 
GAAGGGACAG TAGAAATCAA ATTCCGCAGA AAGGATCTGG TGAAAACCAT GCGTCGGGTG 
GACCCAGTCT ACATTCGCTT GGCTGAGCGA TTGGGCACCC CAGAGCTCAG CGCAGCTGAG 
CGGAAGGAGC TGGAGAACAA GTTGAAGGAG CGGGAGGAAT TCCTAATTCC CATTTACCAT 
CAGGTAGCCG TGCAGTTTGC TGACTTGCAC GACACACCAG GCCGGATGCA GGAGAAGGGT 
GTTATTAATG ACATCCTGGA TTGGAAAACA TCCCGTACCT TTTTCTATTG GCGGCTGAGG 
CGTCTCCTGC TGGAGGACCT GGTCAAAAAG AAAATCCACA ATGCCAACCC AGAGCTGACC 
GATGGCCAGA TCCAGGCCAT GCTACGGCGC TGGTTTGTAG AAGTGGAAGG AACTGTGAAG 
GCCTACGTTT GGGACAATAA CAAGGACCTG GTGGAGTGGC TGGAGAAACA GCTGACCGAG 
GAAGATGGTG TTCGCTCAGT AATCGAGGAA AACATCAAAT ATATCAGCAG AGACTATGTC 
CTCAAGCAGA TCCGCAGCTT GGTACAGGCC AATCCCGAGG TTGCCATGGA CTCCATCGTC 
CATATGACTC AGCATATCTC ACCCACTCAG CGAGCAGAAG TCGTACGGAT CCTCTCCACG 
ATGGACTCCC CTTCAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_005624779.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624836.1 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X4, mRNA.

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CloneID	OCe12093
Clone ID Related Accession (Same CDS sequence)	XM_005624780.3 , XM_005624780.2 , XM_005624783.2 , XM_005624778.2 , XM_005624779.3 , XM_005624779.2 , XM_005624783.3 , XM_005624778.3
Accession Version	XM_005624779.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	acetyl-CoA carboxylase 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624779.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAC CATCCCCCTT GGCCAAACCT CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGTTCTG TGTCTGAAGA TAACTCAGAG GATGAAATTA GCAACCTGGT GAAGCTGGAT 
CTCCTGGAGG AGAAGGAGGG CTCTCTATCA CCAGCTTCTG TTGGCTCAGA TACACTTTCT 
GACTTGGGGA TCTCCAGCCT ACAGGACGGC TTGGCCTTAC ACATGAGGTC CAGCATGTCT 
GGCTTGCATC TAGTGAAGCA AGGTCGAGAC CGAAAGAAAA TAGACTCTCA GCGAGATTTC 
ACTGTAGCTT CTCCAGCAGA ATTTGTTACT CGTTTTGGGG GAAATAAAGT GATTGAGAAG 
GTTCTTATTG CCAACAATGG CATTGCAGCA GTGAAATGCA TGCGGTCTAT CCGTCGATGG 
TCTTATGAGA TGTTTCGAAA TGAACGTGCA ATCCGATTTG TTGTTATGGT AACACCTGAA 
GACCTGAAAG CCAATGCAGA ATACATTAAG ATGGCGGATC ACTATGTGCC AGTGCCAGGA 
GGACCAAATA ACAACAACTA TGCGAACGTG GAATTAATTC TTGATATTGC TAAAAGGATT 
CCCGTACAAG CAGTGTGGGC TGGCTGGGGT CATGCTTCTG AGAATCCCAA GCTCCCAGAA 
CTTCTTTTGA AAAATGGCAT CGCCTTCATG GGCCCTCCAA GCCAGGCCAT GTGGGCTTTG 
GGGGATAAGA TTGCATCTTC TATAGTGGCT CAAACTGCAG GTATCCCAAC TCTTCCCTGG 
AGTGGCAGTG GTCTTCGGGT GGACTGGCAT GAAAATGATT TTTCCAAACG TATCTTAAAT 
GTTCCCCAGG AATTATACGA AAAAGGTTAT GTGAAGGATG TGGATGATGG GCTAAAGGCA 
GCGGAGGAAG TTGGATATCC AGTAATGATC AAGGCCTCAG AAGGAGGAGG AGGGAAAGGA 
ATCAGAAAAG TCAACAATGC AGATGATTTC CCTAACCTCT TCAGACAGGT TCAAGCTGAA 
GTCCCTGGAT CTCCTATATT TGTGATGAGA CTAGCCAAAC AATCTCGTCA TCTGGAGGTA 
CAGATCTTAG CAGATCAATA TGGCAATGCT ATCTCTCTCT TTGGTCGTGA CTGCTCTGTA 
CAGCGCAGGC ATCAGAAGAT TATTGAGGAA GCACCTGCTG CTATTGCTAC TCCAGCAGTA 
TTTGAACATA TGGAACAGTG TGCTGTGAAA CTTGCCAAAA TGGTTGGTTA TGTGAGTGCT 
GGGACTGTGG AATACCTCTA CAGCCAGGAT GGAAGCTTCT ACTTTCTGGA ACTGAACCCT 
CGGCTACAGG TGGAACACCC TTGTACAGAG ATGGTGGCTG ATGTCAACCT CCCTGCAGCA 
CAACTCCAGA TTGCCATGGG GATCCCTCTG TACAGAATCA AGGATATTCG TATGATGTAT 
GGGGTATCTC CCTGGGGTGA TGCTCCCATT GATTTTGAAA ATTCTGCTCA CGTTCCTTGC 
CCAAGGGGCC ATGTTATTGC CGCTCGGATC ACTAGTGAAA ATCCAGATGA GGGTTTTAAG 
CCCAGCTCAG GAACAGTTCA GGAACTGAAT TTCCGTAGCA ATAAGAATGT TTGGGGTTAT 
TTCAGTGTTG CTGCTGCAGG GGGCCTTCAT GAATTTGCTG ATTCTCAGTT TGGTCATTGC 
TTCTCCTGGG GAGAAAACAG AGAGGAAGCA ATTTCAAACA TGGTGGTGGC TTTGAAGGAG 
CTGTCTATTC GGGGTGACTT TCGAACCACA GTTGAATACC TCATCAAATT GTTGGAGACT 
GAAAGCTTTC AGTTGAATAG AATTGACACC GGCTGGCTGG ACAGACTGAT AGCAGAGAAA 
GTACAGGCAG AGCGACCTGA CACCATGTTG GGAGTTGTTT GTGGGGCTCT CCATGTGGCA 
GATGTGAGCC TACGGAATAG CATCTCTAAC TTCCTTCATT CTTTAGAAAG GGGTCAAGTC 
CTTCCTGCTC ATACACTTCT GAATACAGTA GATGTTGAAC TTATCTATGG GGGAGTCAAA 
TATGTACTTA AGGTGACTCG GCAGTCCCCC AACTCCTATG TGGTGATCAT GAATGGTTCA 
TGCGTAGAAG TAGATGTGCA TCGGCTCAGT GATGGAGGGT TACTCCTGTC CTATGATGGC 
AGCAGTTACA CCACATACAT GAAGGAAGAA GTGGATAGAT ATCGTATCAC AATTGGCAAT 
AAGACCTGTG TGTTTGAGAA GGAAAATGAC CCTTCAGTGT TACGCTCACC TTCTGCTGGG 
AAGTTAATCC AGTACATTGT GGAGGATGGA GGCCATGTGT TTGCTGGCCA GTGCTATGCT 
GAAATTGAGG TGATGAAGAT GGTAATGACC TTAACAGCTG CAGAGTCTGG CTGTATCCAT 
TATGTCAAAC GGCCTGGAGC AGCTCTTGAC CCTGGCTGTG TAATAGCCAA AATGCAGTTG 
GACAACCCCA GCAAGGTCCA ACAGGCTGAG CTTCACACAG GTAGTCTGCC ACGGATCCAG 
AGCACTGCAC TCAGAGGCGA GAAGCTCCAT CGAGTGTTCC ACTATGTCCT GGATAATCTG 
GTCAACGTAA TGAATGGATA CTGCCTTCCA GATCCTTTTT TTAGCAGCAG GGTAAAAGAC 
TGGGTAGAGC GGTTGATGAA GACCCTCAGA GACCCCTCGT TACCTCTCCT AGAATTACAA 
GATATCATGA CCAGTGTCTC TGGCCGTATT CCCCCCAATG TTGAGAAGTC TATCAAGAAG 
GAAATGGCCC AGTATGCCAG CAATATCACA TCAGTCCTCT GTCAGTTTCC CAGCCAGCAG 
ATTGCCAACA TCCTAGATAG CCATGCAGCT ACGTTGAACC GGAAATCTGA GCGGGAAGTC 
TTTTTCATGA ACACTCAGAG CATTGTCCAG CTGGTACAGA GATACCGAAG TGGCATCCGA 
GGCCACATGA AGGCTGTGGT GATGGATCTG CTGCGGCAGT ACCTGCGAGT AGAGACACAG 
TTCCAGAATG GTCACTATGA CAAATGTGTG TTTGCCCTTC GAGAAGAGAA CAAGAGTGAT 
ATGAACACTG TACTGAACTA TATCTTCTCT CATGCTCAAG TCACCAAGAA GAATCTTCTG 
GTCACAATGC TTATTGATCA GTTGTGCGGT AGGGACCCTA CTCTCACTGA TGAGCTGCTG 
AATATTCTCA CAGAGCTAAC TCAACTCAGT AAGACCACCA ATGCCAAAGT AGCACTTCGA 
GCACGCCAGG TTCTTATTGC CTCCCATTTG CCATCATATG AGCTTCGCCA TAACCAAGTA 
GAGTCTATCT TCCTTTCAGC TATTGACATG TATGGACATC AGTTTTGCAT TGAGAACCTG 
CAGAAACTCA TCTTGTCTGA AACATCTATT TTTGATGTCC TACCAAACTT CTTCTATCAC 
AGCAACCAGG TAGTGAGGAT GGCAGCTCTG GAGGTTTATG TTCGAAGGGC TTATATTGCC 
TATGAACTTA ACAGCGTACA ACATCGCCAA CTTAAGGACA ACACCTGTGT GGTGGAATTC 
CAATTCATGC TACCCACATC TCATCCAAAT AGAGGGAACA TCCCCACGCT AAACAGAATG 
TCCTTCTCCT CCAACCTCAA CCACTATGGC ATGACTCATG TAGCTAGTGT CAGCGATGTG 
CTTTTGGACA ACTCATTCAC TCCACCATGT CAACGGATGG GCGGAATGGT CTCTTTTCGG 
ACTTTCGAAG ATTTTGTCAG GATCTTTGAT GAAGTGATGG GCTGCTTCTG TGATTCTCCA 
CCGCAAAGCC CTACATTCCC TGAGGCAGGT CACACATCTC TATACGATGA AGACAAGGTC 
CCCAGGGATG AACCAATTCA CATTCTGAAT GTGGCTATCA AGACTGACTG TGATATTGAG 
GATGACAGAC TGGCAGCTAT GTTCAGAGAG TTTACCCAAC AAAATAAAGC TACCCTGGTT 
GAGCATGGGA TACGGCGCCT TACTTTCCTG GTTGCACAAA AGGATTTCAG GAAACAGGTC 
AACTATGAGG TGGATCAGAG ATTTCATAGA GAATTTCCTA AATTTTTCAC CTTCCGAGCA 
AGGGATAAGT TTGAGGAGGA TCGTATCTAT CGTCACCTGG AACCTGCCCT CGCTTTCCAG 
TTAGAGCTGA ACCGGATGAG AAATTTTGAC CTTACTGCCA TTCCATGTGC TAATCATAAA 
ATGCATCTGT ATCTTGGGGC AGCCAAGGTG GAGGTGGGCA CAGAAGTGAC AGACTACAGG 
TTCTTTGTTC GTGCAATCAT CAGACATTCT GATCTGGTCA CCAAGGAAGC TTCTTTTGAA 
TACCTACAAA ATGAAGGGGA GAGGCTGCTT CTAGAAGCCA TGGATGAGTT GGAAGTTGCT 
TTTAACAATA CAAACGTTCG CACTGACTGT AACCACATCT TCCTCAACTT TGTGCCCACG 
GTCATCATGG ACCCATCAAA GATTGAGGAA TCTGTGCGGA GCATGGTAAT GCGCTATGGA 
AGTCGGCTGT GGAAATTACG TGTCCTCCAG GCAGAACTGA AAATCAACAT TCGCCTGACA 
CCAACTGGAA AAGCAATTCC CATCCGCCTC TTCCTGACAA ACGAGTCTGG CTATTACTTG 
GACATCAGCT TGTATAAGGA AGTGACTGAC TCCAGGACAG CACAGATCAT GTTTCAGGCA 
TATGGAGACA AACAGGGACC ATTGCATGGA ATGTTAATCA ACACTCCATA CGTCACCAAA 
GACCTGCTTC AATCAAAGAG ATTCCAGGCA CAATCCTTGG GGACAACATA CATATATGAT 
ATACCAGAGA TGTTTCGGCA GTCTCTGATC AAACTCTGGG AGTCTATGTC CACTCAAGCA 
TTTCTTCCAT CACCCCCCCT GCCTTCCGAC ATGCTGACAT ATACGGAGCT CGTGTTGGAT 
GATCAAGGTC AGCTGGTCCA CATGAACAGG CTTCCAGGAG GAAATGAGAT TGGCATGGTA 
GCGTGGAAAA TGACCCTTAA AAGTCCTGAA TACCCAGAAG GCCGAGATAT CATTGTTATT 
GGCAATGACA TCACATACCG AATTGGGTCC TTTGGGCCCC AGGAGGATTT GCTGTTTCTC 
AGAGCTTCTG AGCTTGCCAG GGCAGAGGGT ATCCCACGCA TCTTTGTAGC AGCCAACAGT 
GGAGCAAGAA TTGGACTGGC AGAGGAAATT CGTCATATGT TTCATGTAGC CTGGGTAGAT 
CCTGAGGATC CTTACAAGGG ATACAAATAT TTATATCTGA CCCCTCAGGA TTATAAGAGA 
GTCAGTGCTC TCAACTCTGT CCATTGTGAA CATGTAGAGG ACGAAGGAGA ATCCAGGTAC 
AAGATAACTG ATATTATTGG GAAGGAAGAG GGACTTGGAG CAGAGAACCT TCGAGGCTCT 
GGAATGATCG CTGGAGAATC CTCATTGGCC TATGATGAGA TCATCACCAT CAGCCTGGTT 
ACATGCCGGG CCATTGGGAT TGGAGCTTAC CTTGTCCGGC TGGGACAGAG AACCATCCAG 
GTTGAAAATT CTCATTTAAT TTTGACTGGA GCTGGAGCCC TCAACAAAGT CCTTGGGCGG 
GAAGTATACA CCTCCAATAA CCAGCTCGGG GGCATCCAGA TCATGCACAA CAATGGGGTG 
ACCCACAGCA TTGTTTGTGA TGACTTTGAA GGGGTTTTCA CTGTCCTGCA CTGGCTATCC 
TACATGCCTA AGAATGTGCG CAGTTTAGTT CCTCTCCTGA ACTCCAAGGA TCCTATAGAT 
AGAATCATCG AGTTTGTTCC CACGAAGGCC CCATACGATC CTCGATGGAT GCTAGCAGGC 
CGCCCTCACC CAACTCAGAA AGGTCAGTGG CTGAGTGGAT TTTTTGACTA TGGCTCTTTC 
TCAGAGATCA TGCAACCCTG GGCACAAACT GTGGTGGTTG GCAGAGCCAG GCTAGGAGGA 
ATACCTGTGG GAGTAGTTGC TGTAGAAACC CGAACAGTGG AACTAAGTAT CCCAGCCGAT 
CCTGCAAACC TGGATTCTGA AGCCAAGATA ATCCAGCAGG CTGGCCAGGT TTGGTTCCCA 
GACTCTGCGT TTAAGACCTA TCAGGCTATC AAGGACTTCA ACCGTGAAGG ACTGCCTCTG 
ATGGTCTTTG CCAACTGGAG AGGCTTCTCT GGTGGGATGA AAGATATGTA TGACCAAGTG 
CTGAAGTTTG GTGCCTATAT TGTGGATAGC TTACGGGAGT GCTCCCAGCC TGTGCTGGTA 
TACATTCCTC CTCAGGCTGA GCTCCGGGGT GGCTCCTGGG TTGTGATTGA CCCCACCATC 
AACCCCCGGC ATATGGAGAT GTACGCTGAC CGAGAAAGCA GGGGATCAGT TCTGGAGCCA 
GAAGGGACAG TAGAAATCAA ATTCCGCAGA AAGGATCTGG TGAAAACCAT GCGTCGGGTG 
GACCCAGTCT ACATTCGCTT GGCTGAGCGA TTGGGCACCC CAGAGCTCAG CGCAGCTGAG 
CGGAAGGAGC TGGAGAACAA GTTGAAGGAG CGGGAGGAAT TCCTAATTCC CATTTACCAT 
CAGGTAGCCG TGCAGTTTGC TGACTTGCAC GACACACCAG GCCGGATGCA GGAGAAGGGT 
GTTATTAATG ACATCCTGGA TTGGAAAACA TCCCGTACCT TTTTCTATTG GCGGCTGAGG 
CGTCTCCTGC TGGAGGACCT GGTCAAAAAG AAAATCCACA ATGCCAACCC AGAGCTGACC 
GATGGCCAGA TCCAGGCCAT GCTACGGCGC TGGTTTGTAG AAGTGGAAGG AACTGTGAAG 
GCCTACGTTT GGGACAATAA CAAGGACCTG GTGGAGTGGC TGGAGAAACA GCTGACCGAG 
GAAGATGGTG TTCGCTCAGT AATCGAGGAA AACATCAAAT ATATCAGCAG AGACTATGTC 
CTCAAGCAGA TCCGCAGCTT GGTACAGGCC AATCCCGAGG TTGCCATGGA CTCCATCGTC 
CATATGACTC AGCATATCTC ACCCACTCAG CGAGCAGAAG TCGTACGGAT CCTCTCCACG 
ATGGACTCCC CTTCAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624836.1
CDS	179..7219
Translation	MDEPSPLAKPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_005624779.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624779.2

ORF Insert Sequence:

ATGGATGAAC CATCCCCCTT GGCCAAACCT CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGTTCTG TGTCTGAAGA TAACTCAGAG GATGAAATTA GCAACCTGGT GAAGCTGGAT 
CTCCTGGAGG AGAAGGAGGG CTCTCTATCA CCAGCTTCTG TTGGCTCAGA TACACTTTCT 
GACTTGGGGA TCTCCAGCCT ACAGGACGGC TTGGCCTTAC ACATGAGGTC CAGCATGTCT 
GGCTTGCATC TAGTGAAGCA AGGTCGAGAC CGAAAGAAAA TAGACTCTCA GCGAGATTTC 
ACTGTAGCTT CTCCAGCAGA ATTTGTTACT CGTTTTGGGG GAAATAAAGT GATTGAGAAG 
GTTCTTATTG CCAACAATGG CATTGCAGCA GTGAAATGCA TGCGGTCTAT CCGTCGATGG 
TCTTATGAGA TGTTTCGAAA TGAACGTGCA ATCCGATTTG TTGTTATGGT AACACCTGAA 
GACCTGAAAG CCAATGCAGA ATACATTAAG ATGGCGGATC ACTATGTGCC AGTGCCAGGA 
GGACCAAATA ACAACAACTA TGCGAACGTG GAATTAATTC TTGATATTGC TAAAAGGATT 
CCCGTACAAG CAGTGTGGGC TGGCTGGGGT CATGCTTCTG AGAATCCCAA GCTCCCAGAA 
CTTCTTTTGA AAAATGGCAT CGCCTTCATG GGCCCTCCAA GCCAGGCCAT GTGGGCTTTG 
GGGGATAAGA TTGCATCTTC TATAGTGGCT CAAACTGCAG GTATCCCAAC TCTTCCCTGG 
AGTGGCAGTG GTCTTCGGGT GGACTGGCAT GAAAATGATT TTTCCAAACG TATCTTAAAT 
GTTCCCCAGG AATTATACGA AAAAGGTTAT GTGAAGGATG TGGATGATGG GCTAAAGGCA 
GCGGAGGAAG TTGGATATCC AGTAATGATC AAGGCCTCAG AAGGAGGAGG AGGGAAAGGA 
ATCAGAAAAG TCAACAATGC AGATGATTTC CCTAACCTCT TCAGACAGGT TCAAGCTGAA 
GTCCCTGGAT CTCCTATATT TGTGATGAGA CTAGCCAAAC AATCTCGTCA TCTGGAGGTA 
CAGATCTTAG CAGATCAATA TGGCAATGCT ATCTCTCTCT TTGGTCGTGA CTGCTCTGTA 
CAGCGCAGGC ATCAGAAGAT TATTGAGGAA GCACCTGCTG CTATTGCTAC TCCAGCAGTA 
TTTGAACATA TGGAACAGTG TGCTGTGAAA CTTGCCAAAA TGGTTGGTTA TGTGAGTGCT 
GGGACTGTGG AATACCTCTA CAGCCAGGAT GGAAGCTTCT ACTTTCTGGA ACTGAACCCT 
CGGCTACAGG TGGAACACCC TTGTACAGAG ATGGTGGCTG ATGTCAACCT CCCTGCAGCA 
CAACTCCAGA TTGCCATGGG GATCCCTCTG TACAGAATCA AGGATATTCG TATGATGTAT 
GGGGTATCTC CCTGGGGTGA TGCTCCCATT GATTTTGAAA ATTCTGCTCA CGTTCCTTGC 
CCAAGGGGCC ATGTTATTGC CGCTCGGATC ACTAGTGAAA ATCCAGATGA GGGTTTTAAG 
CCCAGCTCAG GAACAGTTCA GGAACTGAAT TTCCGTAGCA ATAAGAATGT TTGGGGTTAT 
TTCAGTGTTG CTGCTGCAGG GGGCCTTCAT GAATTTGCTG ATTCTCAGTT TGGTCATTGC 
TTCTCCTGGG GAGAAAACAG AGAGGAAGCA ATTTCAAACA TGGTGGTGGC TTTGAAGGAG 
CTGTCTATTC GGGGTGACTT TCGAACCACA GTTGAATACC TCATCAAATT GTTGGAGACT 
GAAAGCTTTC AGTTGAATAG AATTGACACC GGCTGGCTGG ACAGACTGAT AGCAGAGAAA 
GTACAGGCAG AGCGACCTGA CACCATGTTG GGAGTTGTTT GTGGGGCTCT CCATGTGGCA 
GATGTGAGCC TACGGAATAG CATCTCTAAC TTCCTTCATT CTTTAGAAAG GGGTCAAGTC 
CTTCCTGCTC ATACACTTCT GAATACAGTA GATGTTGAAC TTATCTATGG GGGAGTCAAA 
TATGTACTTA AGGTGACTCG GCAGTCCCCC AACTCCTATG TGGTGATCAT GAATGGTTCA 
TGCGTAGAAG TAGATGTGCA TCGGCTCAGT GATGGAGGGT TACTCCTGTC CTATGATGGC 
AGCAGTTACA CCACATACAT GAAGGAAGAA GTGGATAGAT ATCGTATCAC AATTGGCAAT 
AAGACCTGTG TGTTTGAGAA GGAAAATGAC CCTTCAGTGT TACGCTCACC TTCTGCTGGG 
AAGTTAATCC AGTACATTGT GGAGGATGGA GGCCATGTGT TTGCTGGCCA GTGCTATGCT 
GAAATTGAGG TGATGAAGAT GGTAATGACC TTAACAGCTG CAGAGTCTGG CTGTATCCAT 
TATGTCAAAC GGCCTGGAGC AGCTCTTGAC CCTGGCTGTG TAATAGCCAA AATGCAGTTG 
GACAACCCCA GCAAGGTCCA ACAGGCTGAG CTTCACACAG GTAGTCTGCC ACGGATCCAG 
AGCACTGCAC TCAGAGGCGA GAAGCTCCAT CGAGTGTTCC ACTATGTCCT GGATAATCTG 
GTCAACGTAA TGAATGGATA CTGCCTTCCA GATCCTTTTT TTAGCAGCAG GGTAAAAGAC 
TGGGTAGAGC GGTTGATGAA GACCCTCAGA GACCCCTCGT TACCTCTCCT AGAATTACAA 
GATATCATGA CCAGTGTCTC TGGCCGTATT CCCCCCAATG TTGAGAAGTC TATCAAGAAG 
GAAATGGCCC AGTATGCCAG CAATATCACA TCAGTCCTCT GTCAGTTTCC CAGCCAGCAG 
ATTGCCAACA TCCTAGATAG CCATGCAGCT ACGTTGAACC GGAAATCTGA GCGGGAAGTC 
TTTTTCATGA ACACTCAGAG CATTGTCCAG CTGGTACAGA GATACCGAAG TGGCATCCGA 
GGCCACATGA AGGCTGTGGT GATGGATCTG CTGCGGCAGT ACCTGCGAGT AGAGACACAG 
TTCCAGAATG GTCACTATGA CAAATGTGTG TTTGCCCTTC GAGAAGAGAA CAAGAGTGAT 
ATGAACACTG TACTGAACTA TATCTTCTCT CATGCTCAAG TCACCAAGAA GAATCTTCTG 
GTCACAATGC TTATTGATCA GTTGTGCGGT AGGGACCCTA CTCTCACTGA TGAGCTGCTG 
AATATTCTCA CAGAGCTAAC TCAACTCAGT AAGACCACCA ATGCCAAAGT AGCACTTCGA 
GCACGCCAGG TTCTTATTGC CTCCCATTTG CCATCATATG AGCTTCGCCA TAACCAAGTA 
GAGTCTATCT TCCTTTCAGC TATTGACATG TATGGACATC AGTTTTGCAT TGAGAACCTG 
CAGAAACTCA TCTTGTCTGA AACATCTATT TTTGATGTCC TACCAAACTT CTTCTATCAC 
AGCAACCAGG TAGTGAGGAT GGCAGCTCTG GAGGTTTATG TTCGAAGGGC TTATATTGCC 
TATGAACTTA ACAGCGTACA ACATCGCCAA CTTAAGGACA ACACCTGTGT GGTGGAATTC 
CAATTCATGC TACCCACATC TCATCCAAAT AGAGGGAACA TCCCCACGCT AAACAGAATG 
TCCTTCTCCT CCAACCTCAA CCACTATGGC ATGACTCATG TAGCTAGTGT CAGCGATGTG 
CTTTTGGACA ACTCATTCAC TCCACCATGT CAACGGATGG GCGGAATGGT CTCTTTTCGG 
ACTTTCGAAG ATTTTGTCAG GATCTTTGAT GAAGTGATGG GCTGCTTCTG TGATTCTCCA 
CCGCAAAGCC CTACATTCCC TGAGGCAGGT CACACATCTC TATACGATGA AGACAAGGTC 
CCCAGGGATG AACCAATTCA CATTCTGAAT GTGGCTATCA AGACTGACTG TGATATTGAG 
GATGACAGAC TGGCAGCTAT GTTCAGAGAG TTTACCCAAC AAAATAAAGC TACCCTGGTT 
GAGCATGGGA TACGGCGCCT TACTTTCCTG GTTGCACAAA AGGATTTCAG GAAACAGGTC 
AACTATGAGG TGGATCAGAG ATTTCATAGA GAATTTCCTA AATTTTTCAC CTTCCGAGCA 
AGGGATAAGT TTGAGGAGGA TCGTATCTAT CGTCACCTGG AACCTGCCCT CGCTTTCCAG 
TTAGAGCTGA ACCGGATGAG AAATTTTGAC CTTACTGCCA TTCCATGTGC TAATCATAAA 
ATGCATCTGT ATCTTGGGGC AGCCAAGGTG GAGGTGGGCA CAGAAGTGAC AGACTACAGG 
TTCTTTGTTC GTGCAATCAT CAGACATTCT GATCTGGTCA CCAAGGAAGC TTCTTTTGAA 
TACCTACAAA ATGAAGGGGA GAGGCTGCTT CTAGAAGCCA TGGATGAGTT GGAAGTTGCT 
TTTAACAATA CAAACGTTCG CACTGACTGT AACCACATCT TCCTCAACTT TGTGCCCACG 
GTCATCATGG ACCCATCAAA GATTGAGGAA TCTGTGCGGA GCATGGTAAT GCGCTATGGA 
AGTCGGCTGT GGAAATTACG TGTCCTCCAG GCAGAACTGA AAATCAACAT TCGCCTGACA 
CCAACTGGAA AAGCAATTCC CATCCGCCTC TTCCTGACAA ACGAGTCTGG CTATTACTTG 
GACATCAGCT TGTATAAGGA AGTGACTGAC TCCAGGACAG CACAGATCAT GTTTCAGGCA 
TATGGAGACA AACAGGGACC ATTGCATGGA ATGTTAATCA ACACTCCATA CGTCACCAAA 
GACCTGCTTC AATCAAAGAG ATTCCAGGCA CAATCCTTGG GGACAACATA CATATATGAT 
ATACCAGAGA TGTTTCGGCA GTCTCTGATC AAACTCTGGG AGTCTATGTC CACTCAAGCA 
TTTCTTCCAT CACCCCCCCT GCCTTCCGAC ATGCTGACAT ATACGGAGCT CGTGTTGGAT 
GATCAAGGTC AGCTGGTCCA CATGAACAGG CTTCCAGGAG GAAATGAGAT TGGCATGGTA 
GCGTGGAAAA TGACCCTTAA AAGTCCTGAA TACCCAGAAG GCCGAGATAT CATTGTTATT 
GGCAATGACA TCACATACCG AATTGGGTCC TTTGGGCCCC AGGAGGATTT GCTGTTTCTC 
AGAGCTTCTG AGCTTGCCAG GGCAGAGGGT ATCCCACGCA TCTTTGTAGC AGCCAACAGT 
GGAGCAAGAA TTGGACTGGC AGAGGAAATT CGTCATATGT TTCATGTAGC CTGGGTAGAT 
CCTGAGGATC CTTACAAGGG ATACAAATAT TTATATCTGA CCCCTCAGGA TTATAAGAGA 
GTCAGTGCTC TCAACTCTGT CCATTGTGAA CATGTAGAGG ACGAAGGAGA ATCCAGGTAC 
AAGATAACTG ATATTATTGG GAAGGAAGAG GGACTTGGAG CAGAGAACCT TCGAGGCTCT 
GGAATGATCG CTGGAGAATC CTCATTGGCC TATGATGAGA TCATCACCAT CAGCCTGGTT 
ACATGCCGGG CCATTGGGAT TGGAGCTTAC CTTGTCCGGC TGGGACAGAG AACCATCCAG 
GTTGAAAATT CTCATTTAAT TTTGACTGGA GCTGGAGCCC TCAACAAAGT CCTTGGGCGG 
GAAGTATACA CCTCCAATAA CCAGCTCGGG GGCATCCAGA TCATGCACAA CAATGGGGTG 
ACCCACAGCA TTGTTTGTGA TGACTTTGAA GGGGTTTTCA CTGTCCTGCA CTGGCTATCC 
TACATGCCTA AGAATGTGCG CAGTTTAGTT CCTCTCCTGA ACTCCAAGGA TCCTATAGAT 
AGAATCATCG AGTTTGTTCC CACGAAGGCC CCATACGATC CTCGATGGAT GCTAGCAGGC 
CGCCCTCACC CAACTCAGAA AGGTCAGTGG CTGAGTGGAT TTTTTGACTA TGGCTCTTTC 
TCAGAGATCA TGCAACCCTG GGCACAAACT GTGGTGGTTG GCAGAGCCAG GCTAGGAGGA 
ATACCTGTGG GAGTAGTTGC TGTAGAAACC CGAACAGTGG AACTAAGTAT CCCAGCCGAT 
CCTGCAAACC TGGATTCTGA AGCCAAGATA ATCCAGCAGG CTGGCCAGGT TTGGTTCCCA 
GACTCTGCGT TTAAGACCTA TCAGGCTATC AAGGACTTCA ACCGTGAAGG ACTGCCTCTG 
ATGGTCTTTG CCAACTGGAG AGGCTTCTCT GGTGGGATGA AAGATATGTA TGACCAAGTG 
CTGAAGTTTG GTGCCTATAT TGTGGATAGC TTACGGGAGT GCTCCCAGCC TGTGCTGGTA 
TACATTCCTC CTCAGGCTGA GCTCCGGGGT GGCTCCTGGG TTGTGATTGA CCCCACCATC 
AACCCCCGGC ATATGGAGAT GTACGCTGAC CGAGAAAGCA GGGGATCAGT TCTGGAGCCA 
GAAGGGACAG TAGAAATCAA ATTCCGCAGA AAGGATCTGG TGAAAACCAT GCGTCGGGTG 
GACCCAGTCT ACATTCGCTT GGCTGAGCGA TTGGGCACCC CAGAGCTCAG CGCAGCTGAG 
CGGAAGGAGC TGGAGAACAA GTTGAAGGAG CGGGAGGAAT TCCTAATTCC CATTTACCAT 
CAGGTAGCCG TGCAGTTTGC TGACTTGCAC GACACACCAG GCCGGATGCA GGAGAAGGGT 
GTTATTAATG ACATCCTGGA TTGGAAAACA TCCCGTACCT TTTTCTATTG GCGGCTGAGG 
CGTCTCCTGC TGGAGGACCT GGTCAAAAAG AAAATCCACA ATGCCAACCC AGAGCTGACC 
GATGGCCAGA TCCAGGCCAT GCTACGGCGC TGGTTTGTAG AAGTGGAAGG AACTGTGAAG 
GCCTACGTTT GGGACAATAA CAAGGACCTG GTGGAGTGGC TGGAGAAACA GCTGACCGAG 
GAAGATGGTG TTCGCTCAGT AATCGAGGAA AACATCAAAT ATATCAGCAG AGACTATGTC 
CTCAAGCAGA TCCGCAGCTT GGTACAGGCC AATCCCGAGG TTGCCATGGA CTCCATCGTC 
CATATGACTC AGCATATCTC ACCCACTCAG CGAGCAGAAG TCGTACGGAT CCTCTCCACG 
ATGGACTCCC CTTCAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_005624783.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624840.1 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X5, mRNA.

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CloneID	OCe12093
Clone ID Related Accession (Same CDS sequence)	XM_005624780.3 , XM_005624780.2 , XM_005624783.2 , XM_005624778.2 , XM_005624779.3 , XM_005624779.2 , XM_005624783.3 , XM_005624778.3
Accession Version	XM_005624783.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	acetyl-CoA carboxylase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624783.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAC CATCCCCCTT GGCCAAACCT CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGTTCTG TGTCTGAAGA TAACTCAGAG GATGAAATTA GCAACCTGGT GAAGCTGGAT 
CTCCTGGAGG AGAAGGAGGG CTCTCTATCA CCAGCTTCTG TTGGCTCAGA TACACTTTCT 
GACTTGGGGA TCTCCAGCCT ACAGGACGGC TTGGCCTTAC ACATGAGGTC CAGCATGTCT 
GGCTTGCATC TAGTGAAGCA AGGTCGAGAC CGAAAGAAAA TAGACTCTCA GCGAGATTTC 
ACTGTAGCTT CTCCAGCAGA ATTTGTTACT CGTTTTGGGG GAAATAAAGT GATTGAGAAG 
GTTCTTATTG CCAACAATGG CATTGCAGCA GTGAAATGCA TGCGGTCTAT CCGTCGATGG 
TCTTATGAGA TGTTTCGAAA TGAACGTGCA ATCCGATTTG TTGTTATGGT AACACCTGAA 
GACCTGAAAG CCAATGCAGA ATACATTAAG ATGGCGGATC ACTATGTGCC AGTGCCAGGA 
GGACCAAATA ACAACAACTA TGCGAACGTG GAATTAATTC TTGATATTGC TAAAAGGATT 
CCCGTACAAG CAGTGTGGGC TGGCTGGGGT CATGCTTCTG AGAATCCCAA GCTCCCAGAA 
CTTCTTTTGA AAAATGGCAT CGCCTTCATG GGCCCTCCAA GCCAGGCCAT GTGGGCTTTG 
GGGGATAAGA TTGCATCTTC TATAGTGGCT CAAACTGCAG GTATCCCAAC TCTTCCCTGG 
AGTGGCAGTG GTCTTCGGGT GGACTGGCAT GAAAATGATT TTTCCAAACG TATCTTAAAT 
GTTCCCCAGG AATTATACGA AAAAGGTTAT GTGAAGGATG TGGATGATGG GCTAAAGGCA 
GCGGAGGAAG TTGGATATCC AGTAATGATC AAGGCCTCAG AAGGAGGAGG AGGGAAAGGA 
ATCAGAAAAG TCAACAATGC AGATGATTTC CCTAACCTCT TCAGACAGGT TCAAGCTGAA 
GTCCCTGGAT CTCCTATATT TGTGATGAGA CTAGCCAAAC AATCTCGTCA TCTGGAGGTA 
CAGATCTTAG CAGATCAATA TGGCAATGCT ATCTCTCTCT TTGGTCGTGA CTGCTCTGTA 
CAGCGCAGGC ATCAGAAGAT TATTGAGGAA GCACCTGCTG CTATTGCTAC TCCAGCAGTA 
TTTGAACATA TGGAACAGTG TGCTGTGAAA CTTGCCAAAA TGGTTGGTTA TGTGAGTGCT 
GGGACTGTGG AATACCTCTA CAGCCAGGAT GGAAGCTTCT ACTTTCTGGA ACTGAACCCT 
CGGCTACAGG TGGAACACCC TTGTACAGAG ATGGTGGCTG ATGTCAACCT CCCTGCAGCA 
CAACTCCAGA TTGCCATGGG GATCCCTCTG TACAGAATCA AGGATATTCG TATGATGTAT 
GGGGTATCTC CCTGGGGTGA TGCTCCCATT GATTTTGAAA ATTCTGCTCA CGTTCCTTGC 
CCAAGGGGCC ATGTTATTGC CGCTCGGATC ACTAGTGAAA ATCCAGATGA GGGTTTTAAG 
CCCAGCTCAG GAACAGTTCA GGAACTGAAT TTCCGTAGCA ATAAGAATGT TTGGGGTTAT 
TTCAGTGTTG CTGCTGCAGG GGGCCTTCAT GAATTTGCTG ATTCTCAGTT TGGTCATTGC 
TTCTCCTGGG GAGAAAACAG AGAGGAAGCA ATTTCAAACA TGGTGGTGGC TTTGAAGGAG 
CTGTCTATTC GGGGTGACTT TCGAACCACA GTTGAATACC TCATCAAATT GTTGGAGACT 
GAAAGCTTTC AGTTGAATAG AATTGACACC GGCTGGCTGG ACAGACTGAT AGCAGAGAAA 
GTACAGGCAG AGCGACCTGA CACCATGTTG GGAGTTGTTT GTGGGGCTCT CCATGTGGCA 
GATGTGAGCC TACGGAATAG CATCTCTAAC TTCCTTCATT CTTTAGAAAG GGGTCAAGTC 
CTTCCTGCTC ATACACTTCT GAATACAGTA GATGTTGAAC TTATCTATGG GGGAGTCAAA 
TATGTACTTA AGGTGACTCG GCAGTCCCCC AACTCCTATG TGGTGATCAT GAATGGTTCA 
TGCGTAGAAG TAGATGTGCA TCGGCTCAGT GATGGAGGGT TACTCCTGTC CTATGATGGC 
AGCAGTTACA CCACATACAT GAAGGAAGAA GTGGATAGAT ATCGTATCAC AATTGGCAAT 
AAGACCTGTG TGTTTGAGAA GGAAAATGAC CCTTCAGTGT TACGCTCACC TTCTGCTGGG 
AAGTTAATCC AGTACATTGT GGAGGATGGA GGCCATGTGT TTGCTGGCCA GTGCTATGCT 
GAAATTGAGG TGATGAAGAT GGTAATGACC TTAACAGCTG CAGAGTCTGG CTGTATCCAT 
TATGTCAAAC GGCCTGGAGC AGCTCTTGAC CCTGGCTGTG TAATAGCCAA AATGCAGTTG 
GACAACCCCA GCAAGGTCCA ACAGGCTGAG CTTCACACAG GTAGTCTGCC ACGGATCCAG 
AGCACTGCAC TCAGAGGCGA GAAGCTCCAT CGAGTGTTCC ACTATGTCCT GGATAATCTG 
GTCAACGTAA TGAATGGATA CTGCCTTCCA GATCCTTTTT TTAGCAGCAG GGTAAAAGAC 
TGGGTAGAGC GGTTGATGAA GACCCTCAGA GACCCCTCGT TACCTCTCCT AGAATTACAA 
GATATCATGA CCAGTGTCTC TGGCCGTATT CCCCCCAATG TTGAGAAGTC TATCAAGAAG 
GAAATGGCCC AGTATGCCAG CAATATCACA TCAGTCCTCT GTCAGTTTCC CAGCCAGCAG 
ATTGCCAACA TCCTAGATAG CCATGCAGCT ACGTTGAACC GGAAATCTGA GCGGGAAGTC 
TTTTTCATGA ACACTCAGAG CATTGTCCAG CTGGTACAGA GATACCGAAG TGGCATCCGA 
GGCCACATGA AGGCTGTGGT GATGGATCTG CTGCGGCAGT ACCTGCGAGT AGAGACACAG 
TTCCAGAATG GTCACTATGA CAAATGTGTG TTTGCCCTTC GAGAAGAGAA CAAGAGTGAT 
ATGAACACTG TACTGAACTA TATCTTCTCT CATGCTCAAG TCACCAAGAA GAATCTTCTG 
GTCACAATGC TTATTGATCA GTTGTGCGGT AGGGACCCTA CTCTCACTGA TGAGCTGCTG 
AATATTCTCA CAGAGCTAAC TCAACTCAGT AAGACCACCA ATGCCAAAGT AGCACTTCGA 
GCACGCCAGG TTCTTATTGC CTCCCATTTG CCATCATATG AGCTTCGCCA TAACCAAGTA 
GAGTCTATCT TCCTTTCAGC TATTGACATG TATGGACATC AGTTTTGCAT TGAGAACCTG 
CAGAAACTCA TCTTGTCTGA AACATCTATT TTTGATGTCC TACCAAACTT CTTCTATCAC 
AGCAACCAGG TAGTGAGGAT GGCAGCTCTG GAGGTTTATG TTCGAAGGGC TTATATTGCC 
TATGAACTTA ACAGCGTACA ACATCGCCAA CTTAAGGACA ACACCTGTGT GGTGGAATTC 
CAATTCATGC TACCCACATC TCATCCAAAT AGAGGGAACA TCCCCACGCT AAACAGAATG 
TCCTTCTCCT CCAACCTCAA CCACTATGGC ATGACTCATG TAGCTAGTGT CAGCGATGTG 
CTTTTGGACA ACTCATTCAC TCCACCATGT CAACGGATGG GCGGAATGGT CTCTTTTCGG 
ACTTTCGAAG ATTTTGTCAG GATCTTTGAT GAAGTGATGG GCTGCTTCTG TGATTCTCCA 
CCGCAAAGCC CTACATTCCC TGAGGCAGGT CACACATCTC TATACGATGA AGACAAGGTC 
CCCAGGGATG AACCAATTCA CATTCTGAAT GTGGCTATCA AGACTGACTG TGATATTGAG 
GATGACAGAC TGGCAGCTAT GTTCAGAGAG TTTACCCAAC AAAATAAAGC TACCCTGGTT 
GAGCATGGGA TACGGCGCCT TACTTTCCTG GTTGCACAAA AGGATTTCAG GAAACAGGTC 
AACTATGAGG TGGATCAGAG ATTTCATAGA GAATTTCCTA AATTTTTCAC CTTCCGAGCA 
AGGGATAAGT TTGAGGAGGA TCGTATCTAT CGTCACCTGG AACCTGCCCT CGCTTTCCAG 
TTAGAGCTGA ACCGGATGAG AAATTTTGAC CTTACTGCCA TTCCATGTGC TAATCATAAA 
ATGCATCTGT ATCTTGGGGC AGCCAAGGTG GAGGTGGGCA CAGAAGTGAC AGACTACAGG 
TTCTTTGTTC GTGCAATCAT CAGACATTCT GATCTGGTCA CCAAGGAAGC TTCTTTTGAA 
TACCTACAAA ATGAAGGGGA GAGGCTGCTT CTAGAAGCCA TGGATGAGTT GGAAGTTGCT 
TTTAACAATA CAAACGTTCG CACTGACTGT AACCACATCT TCCTCAACTT TGTGCCCACG 
GTCATCATGG ACCCATCAAA GATTGAGGAA TCTGTGCGGA GCATGGTAAT GCGCTATGGA 
AGTCGGCTGT GGAAATTACG TGTCCTCCAG GCAGAACTGA AAATCAACAT TCGCCTGACA 
CCAACTGGAA AAGCAATTCC CATCCGCCTC TTCCTGACAA ACGAGTCTGG CTATTACTTG 
GACATCAGCT TGTATAAGGA AGTGACTGAC TCCAGGACAG CACAGATCAT GTTTCAGGCA 
TATGGAGACA AACAGGGACC ATTGCATGGA ATGTTAATCA ACACTCCATA CGTCACCAAA 
GACCTGCTTC AATCAAAGAG ATTCCAGGCA CAATCCTTGG GGACAACATA CATATATGAT 
ATACCAGAGA TGTTTCGGCA GTCTCTGATC AAACTCTGGG AGTCTATGTC CACTCAAGCA 
TTTCTTCCAT CACCCCCCCT GCCTTCCGAC ATGCTGACAT ATACGGAGCT CGTGTTGGAT 
GATCAAGGTC AGCTGGTCCA CATGAACAGG CTTCCAGGAG GAAATGAGAT TGGCATGGTA 
GCGTGGAAAA TGACCCTTAA AAGTCCTGAA TACCCAGAAG GCCGAGATAT CATTGTTATT 
GGCAATGACA TCACATACCG AATTGGGTCC TTTGGGCCCC AGGAGGATTT GCTGTTTCTC 
AGAGCTTCTG AGCTTGCCAG GGCAGAGGGT ATCCCACGCA TCTTTGTAGC AGCCAACAGT 
GGAGCAAGAA TTGGACTGGC AGAGGAAATT CGTCATATGT TTCATGTAGC CTGGGTAGAT 
CCTGAGGATC CTTACAAGGG ATACAAATAT TTATATCTGA CCCCTCAGGA TTATAAGAGA 
GTCAGTGCTC TCAACTCTGT CCATTGTGAA CATGTAGAGG ACGAAGGAGA ATCCAGGTAC 
AAGATAACTG ATATTATTGG GAAGGAAGAG GGACTTGGAG CAGAGAACCT TCGAGGCTCT 
GGAATGATCG CTGGAGAATC CTCATTGGCC TATGATGAGA TCATCACCAT CAGCCTGGTT 
ACATGCCGGG CCATTGGGAT TGGAGCTTAC CTTGTCCGGC TGGGACAGAG AACCATCCAG 
GTTGAAAATT CTCATTTAAT TTTGACTGGA GCTGGAGCCC TCAACAAAGT CCTTGGGCGG 
GAAGTATACA CCTCCAATAA CCAGCTCGGG GGCATCCAGA TCATGCACAA CAATGGGGTG 
ACCCACAGCA TTGTTTGTGA TGACTTTGAA GGGGTTTTCA CTGTCCTGCA CTGGCTATCC 
TACATGCCTA AGAATGTGCG CAGTTTAGTT CCTCTCCTGA ACTCCAAGGA TCCTATAGAT 
AGAATCATCG AGTTTGTTCC CACGAAGGCC CCATACGATC CTCGATGGAT GCTAGCAGGC 
CGCCCTCACC CAACTCAGAA AGGTCAGTGG CTGAGTGGAT TTTTTGACTA TGGCTCTTTC 
TCAGAGATCA TGCAACCCTG GGCACAAACT GTGGTGGTTG GCAGAGCCAG GCTAGGAGGA 
ATACCTGTGG GAGTAGTTGC TGTAGAAACC CGAACAGTGG AACTAAGTAT CCCAGCCGAT 
CCTGCAAACC TGGATTCTGA AGCCAAGATA ATCCAGCAGG CTGGCCAGGT TTGGTTCCCA 
GACTCTGCGT TTAAGACCTA TCAGGCTATC AAGGACTTCA ACCGTGAAGG ACTGCCTCTG 
ATGGTCTTTG CCAACTGGAG AGGCTTCTCT GGTGGGATGA AAGATATGTA TGACCAAGTG 
CTGAAGTTTG GTGCCTATAT TGTGGATAGC TTACGGGAGT GCTCCCAGCC TGTGCTGGTA 
TACATTCCTC CTCAGGCTGA GCTCCGGGGT GGCTCCTGGG TTGTGATTGA CCCCACCATC 
AACCCCCGGC ATATGGAGAT GTACGCTGAC CGAGAAAGCA GGGGATCAGT TCTGGAGCCA 
GAAGGGACAG TAGAAATCAA ATTCCGCAGA AAGGATCTGG TGAAAACCAT GCGTCGGGTG 
GACCCAGTCT ACATTCGCTT GGCTGAGCGA TTGGGCACCC CAGAGCTCAG CGCAGCTGAG 
CGGAAGGAGC TGGAGAACAA GTTGAAGGAG CGGGAGGAAT TCCTAATTCC CATTTACCAT 
CAGGTAGCCG TGCAGTTTGC TGACTTGCAC GACACACCAG GCCGGATGCA GGAGAAGGGT 
GTTATTAATG ACATCCTGGA TTGGAAAACA TCCCGTACCT TTTTCTATTG GCGGCTGAGG 
CGTCTCCTGC TGGAGGACCT GGTCAAAAAG AAAATCCACA ATGCCAACCC AGAGCTGACC 
GATGGCCAGA TCCAGGCCAT GCTACGGCGC TGGTTTGTAG AAGTGGAAGG AACTGTGAAG 
GCCTACGTTT GGGACAATAA CAAGGACCTG GTGGAGTGGC TGGAGAAACA GCTGACCGAG 
GAAGATGGTG TTCGCTCAGT AATCGAGGAA AACATCAAAT ATATCAGCAG AGACTATGTC 
CTCAAGCAGA TCCGCAGCTT GGTACAGGCC AATCCCGAGG TTGCCATGGA CTCCATCGTC 
CATATGACTC AGCATATCTC ACCCACTCAG CGAGCAGAAG TCGTACGGAT CCTCTCCACG 
ATGGACTCCC CTTCAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624840.1
CDS	202..7242
Translation	MDEPSPLAKPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_005624783.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624783.3

ORF Insert Sequence:

ATGGATGAAC CATCCCCCTT GGCCAAACCT CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGTTCTG TGTCTGAAGA TAACTCAGAG GATGAAATTA GCAACCTGGT GAAGCTGGAT 
CTCCTGGAGG AGAAGGAGGG CTCTCTATCA CCAGCTTCTG TTGGCTCAGA TACACTTTCT 
GACTTGGGGA TCTCCAGCCT ACAGGACGGC TTGGCCTTAC ACATGAGGTC CAGCATGTCT 
GGCTTGCATC TAGTGAAGCA AGGTCGAGAC CGAAAGAAAA TAGACTCTCA GCGAGATTTC 
ACTGTAGCTT CTCCAGCAGA ATTTGTTACT CGTTTTGGGG GAAATAAAGT GATTGAGAAG 
GTTCTTATTG CCAACAATGG CATTGCAGCA GTGAAATGCA TGCGGTCTAT CCGTCGATGG 
TCTTATGAGA TGTTTCGAAA TGAACGTGCA ATCCGATTTG TTGTTATGGT AACACCTGAA 
GACCTGAAAG CCAATGCAGA ATACATTAAG ATGGCGGATC ACTATGTGCC AGTGCCAGGA 
GGACCAAATA ACAACAACTA TGCGAACGTG GAATTAATTC TTGATATTGC TAAAAGGATT 
CCCGTACAAG CAGTGTGGGC TGGCTGGGGT CATGCTTCTG AGAATCCCAA GCTCCCAGAA 
CTTCTTTTGA AAAATGGCAT CGCCTTCATG GGCCCTCCAA GCCAGGCCAT GTGGGCTTTG 
GGGGATAAGA TTGCATCTTC TATAGTGGCT CAAACTGCAG GTATCCCAAC TCTTCCCTGG 
AGTGGCAGTG GTCTTCGGGT GGACTGGCAT GAAAATGATT TTTCCAAACG TATCTTAAAT 
GTTCCCCAGG AATTATACGA AAAAGGTTAT GTGAAGGATG TGGATGATGG GCTAAAGGCA 
GCGGAGGAAG TTGGATATCC AGTAATGATC AAGGCCTCAG AAGGAGGAGG AGGGAAAGGA 
ATCAGAAAAG TCAACAATGC AGATGATTTC CCTAACCTCT TCAGACAGGT TCAAGCTGAA 
GTCCCTGGAT CTCCTATATT TGTGATGAGA CTAGCCAAAC AATCTCGTCA TCTGGAGGTA 
CAGATCTTAG CAGATCAATA TGGCAATGCT ATCTCTCTCT TTGGTCGTGA CTGCTCTGTA 
CAGCGCAGGC ATCAGAAGAT TATTGAGGAA GCACCTGCTG CTATTGCTAC TCCAGCAGTA 
TTTGAACATA TGGAACAGTG TGCTGTGAAA CTTGCCAAAA TGGTTGGTTA TGTGAGTGCT 
GGGACTGTGG AATACCTCTA CAGCCAGGAT GGAAGCTTCT ACTTTCTGGA ACTGAACCCT 
CGGCTACAGG TGGAACACCC TTGTACAGAG ATGGTGGCTG ATGTCAACCT CCCTGCAGCA 
CAACTCCAGA TTGCCATGGG GATCCCTCTG TACAGAATCA AGGATATTCG TATGATGTAT 
GGGGTATCTC CCTGGGGTGA TGCTCCCATT GATTTTGAAA ATTCTGCTCA CGTTCCTTGC 
CCAAGGGGCC ATGTTATTGC CGCTCGGATC ACTAGTGAAA ATCCAGATGA GGGTTTTAAG 
CCCAGCTCAG GAACAGTTCA GGAACTGAAT TTCCGTAGCA ATAAGAATGT TTGGGGTTAT 
TTCAGTGTTG CTGCTGCAGG GGGCCTTCAT GAATTTGCTG ATTCTCAGTT TGGTCATTGC 
TTCTCCTGGG GAGAAAACAG AGAGGAAGCA ATTTCAAACA TGGTGGTGGC TTTGAAGGAG 
CTGTCTATTC GGGGTGACTT TCGAACCACA GTTGAATACC TCATCAAATT GTTGGAGACT 
GAAAGCTTTC AGTTGAATAG AATTGACACC GGCTGGCTGG ACAGACTGAT AGCAGAGAAA 
GTACAGGCAG AGCGACCTGA CACCATGTTG GGAGTTGTTT GTGGGGCTCT CCATGTGGCA 
GATGTGAGCC TACGGAATAG CATCTCTAAC TTCCTTCATT CTTTAGAAAG GGGTCAAGTC 
CTTCCTGCTC ATACACTTCT GAATACAGTA GATGTTGAAC TTATCTATGG GGGAGTCAAA 
TATGTACTTA AGGTGACTCG GCAGTCCCCC AACTCCTATG TGGTGATCAT GAATGGTTCA 
TGCGTAGAAG TAGATGTGCA TCGGCTCAGT GATGGAGGGT TACTCCTGTC CTATGATGGC 
AGCAGTTACA CCACATACAT GAAGGAAGAA GTGGATAGAT ATCGTATCAC AATTGGCAAT 
AAGACCTGTG TGTTTGAGAA GGAAAATGAC CCTTCAGTGT TACGCTCACC TTCTGCTGGG 
AAGTTAATCC AGTACATTGT GGAGGATGGA GGCCATGTGT TTGCTGGCCA GTGCTATGCT 
GAAATTGAGG TGATGAAGAT GGTAATGACC TTAACAGCTG CAGAGTCTGG CTGTATCCAT 
TATGTCAAAC GGCCTGGAGC AGCTCTTGAC CCTGGCTGTG TAATAGCCAA AATGCAGTTG 
GACAACCCCA GCAAGGTCCA ACAGGCTGAG CTTCACACAG GTAGTCTGCC ACGGATCCAG 
AGCACTGCAC TCAGAGGCGA GAAGCTCCAT CGAGTGTTCC ACTATGTCCT GGATAATCTG 
GTCAACGTAA TGAATGGATA CTGCCTTCCA GATCCTTTTT TTAGCAGCAG GGTAAAAGAC 
TGGGTAGAGC GGTTGATGAA GACCCTCAGA GACCCCTCGT TACCTCTCCT AGAATTACAA 
GATATCATGA CCAGTGTCTC TGGCCGTATT CCCCCCAATG TTGAGAAGTC TATCAAGAAG 
GAAATGGCCC AGTATGCCAG CAATATCACA TCAGTCCTCT GTCAGTTTCC CAGCCAGCAG 
ATTGCCAACA TCCTAGATAG CCATGCAGCT ACGTTGAACC GGAAATCTGA GCGGGAAGTC 
TTTTTCATGA ACACTCAGAG CATTGTCCAG CTGGTACAGA GATACCGAAG TGGCATCCGA 
GGCCACATGA AGGCTGTGGT GATGGATCTG CTGCGGCAGT ACCTGCGAGT AGAGACACAG 
TTCCAGAATG GTCACTATGA CAAATGTGTG TTTGCCCTTC GAGAAGAGAA CAAGAGTGAT 
ATGAACACTG TACTGAACTA TATCTTCTCT CATGCTCAAG TCACCAAGAA GAATCTTCTG 
GTCACAATGC TTATTGATCA GTTGTGCGGT AGGGACCCTA CTCTCACTGA TGAGCTGCTG 
AATATTCTCA CAGAGCTAAC TCAACTCAGT AAGACCACCA ATGCCAAAGT AGCACTTCGA 
GCACGCCAGG TTCTTATTGC CTCCCATTTG CCATCATATG AGCTTCGCCA TAACCAAGTA 
GAGTCTATCT TCCTTTCAGC TATTGACATG TATGGACATC AGTTTTGCAT TGAGAACCTG 
CAGAAACTCA TCTTGTCTGA AACATCTATT TTTGATGTCC TACCAAACTT CTTCTATCAC 
AGCAACCAGG TAGTGAGGAT GGCAGCTCTG GAGGTTTATG TTCGAAGGGC TTATATTGCC 
TATGAACTTA ACAGCGTACA ACATCGCCAA CTTAAGGACA ACACCTGTGT GGTGGAATTC 
CAATTCATGC TACCCACATC TCATCCAAAT AGAGGGAACA TCCCCACGCT AAACAGAATG 
TCCTTCTCCT CCAACCTCAA CCACTATGGC ATGACTCATG TAGCTAGTGT CAGCGATGTG 
CTTTTGGACA ACTCATTCAC TCCACCATGT CAACGGATGG GCGGAATGGT CTCTTTTCGG 
ACTTTCGAAG ATTTTGTCAG GATCTTTGAT GAAGTGATGG GCTGCTTCTG TGATTCTCCA 
CCGCAAAGCC CTACATTCCC TGAGGCAGGT CACACATCTC TATACGATGA AGACAAGGTC 
CCCAGGGATG AACCAATTCA CATTCTGAAT GTGGCTATCA AGACTGACTG TGATATTGAG 
GATGACAGAC TGGCAGCTAT GTTCAGAGAG TTTACCCAAC AAAATAAAGC TACCCTGGTT 
GAGCATGGGA TACGGCGCCT TACTTTCCTG GTTGCACAAA AGGATTTCAG GAAACAGGTC 
AACTATGAGG TGGATCAGAG ATTTCATAGA GAATTTCCTA AATTTTTCAC CTTCCGAGCA 
AGGGATAAGT TTGAGGAGGA TCGTATCTAT CGTCACCTGG AACCTGCCCT CGCTTTCCAG 
TTAGAGCTGA ACCGGATGAG AAATTTTGAC CTTACTGCCA TTCCATGTGC TAATCATAAA 
ATGCATCTGT ATCTTGGGGC AGCCAAGGTG GAGGTGGGCA CAGAAGTGAC AGACTACAGG 
TTCTTTGTTC GTGCAATCAT CAGACATTCT GATCTGGTCA CCAAGGAAGC TTCTTTTGAA 
TACCTACAAA ATGAAGGGGA GAGGCTGCTT CTAGAAGCCA TGGATGAGTT GGAAGTTGCT 
TTTAACAATA CAAACGTTCG CACTGACTGT AACCACATCT TCCTCAACTT TGTGCCCACG 
GTCATCATGG ACCCATCAAA GATTGAGGAA TCTGTGCGGA GCATGGTAAT GCGCTATGGA 
AGTCGGCTGT GGAAATTACG TGTCCTCCAG GCAGAACTGA AAATCAACAT TCGCCTGACA 
CCAACTGGAA AAGCAATTCC CATCCGCCTC TTCCTGACAA ACGAGTCTGG CTATTACTTG 
GACATCAGCT TGTATAAGGA AGTGACTGAC TCCAGGACAG CACAGATCAT GTTTCAGGCA 
TATGGAGACA AACAGGGACC ATTGCATGGA ATGTTAATCA ACACTCCATA CGTCACCAAA 
GACCTGCTTC AATCAAAGAG ATTCCAGGCA CAATCCTTGG GGACAACATA CATATATGAT 
ATACCAGAGA TGTTTCGGCA GTCTCTGATC AAACTCTGGG AGTCTATGTC CACTCAAGCA 
TTTCTTCCAT CACCCCCCCT GCCTTCCGAC ATGCTGACAT ATACGGAGCT CGTGTTGGAT 
GATCAAGGTC AGCTGGTCCA CATGAACAGG CTTCCAGGAG GAAATGAGAT TGGCATGGTA 
GCGTGGAAAA TGACCCTTAA AAGTCCTGAA TACCCAGAAG GCCGAGATAT CATTGTTATT 
GGCAATGACA TCACATACCG AATTGGGTCC TTTGGGCCCC AGGAGGATTT GCTGTTTCTC 
AGAGCTTCTG AGCTTGCCAG GGCAGAGGGT ATCCCACGCA TCTTTGTAGC AGCCAACAGT 
GGAGCAAGAA TTGGACTGGC AGAGGAAATT CGTCATATGT TTCATGTAGC CTGGGTAGAT 
CCTGAGGATC CTTACAAGGG ATACAAATAT TTATATCTGA CCCCTCAGGA TTATAAGAGA 
GTCAGTGCTC TCAACTCTGT CCATTGTGAA CATGTAGAGG ACGAAGGAGA ATCCAGGTAC 
AAGATAACTG ATATTATTGG GAAGGAAGAG GGACTTGGAG CAGAGAACCT TCGAGGCTCT 
GGAATGATCG CTGGAGAATC CTCATTGGCC TATGATGAGA TCATCACCAT CAGCCTGGTT 
ACATGCCGGG CCATTGGGAT TGGAGCTTAC CTTGTCCGGC TGGGACAGAG AACCATCCAG 
GTTGAAAATT CTCATTTAAT TTTGACTGGA GCTGGAGCCC TCAACAAAGT CCTTGGGCGG 
GAAGTATACA CCTCCAATAA CCAGCTCGGG GGCATCCAGA TCATGCACAA CAATGGGGTG 
ACCCACAGCA TTGTTTGTGA TGACTTTGAA GGGGTTTTCA CTGTCCTGCA CTGGCTATCC 
TACATGCCTA AGAATGTGCG CAGTTTAGTT CCTCTCCTGA ACTCCAAGGA TCCTATAGAT 
AGAATCATCG AGTTTGTTCC CACGAAGGCC CCATACGATC CTCGATGGAT GCTAGCAGGC 
CGCCCTCACC CAACTCAGAA AGGTCAGTGG CTGAGTGGAT TTTTTGACTA TGGCTCTTTC 
TCAGAGATCA TGCAACCCTG GGCACAAACT GTGGTGGTTG GCAGAGCCAG GCTAGGAGGA 
ATACCTGTGG GAGTAGTTGC TGTAGAAACC CGAACAGTGG AACTAAGTAT CCCAGCCGAT 
CCTGCAAACC TGGATTCTGA AGCCAAGATA ATCCAGCAGG CTGGCCAGGT TTGGTTCCCA 
GACTCTGCGT TTAAGACCTA TCAGGCTATC AAGGACTTCA ACCGTGAAGG ACTGCCTCTG 
ATGGTCTTTG CCAACTGGAG AGGCTTCTCT GGTGGGATGA AAGATATGTA TGACCAAGTG 
CTGAAGTTTG GTGCCTATAT TGTGGATAGC TTACGGGAGT GCTCCCAGCC TGTGCTGGTA 
TACATTCCTC CTCAGGCTGA GCTCCGGGGT GGCTCCTGGG TTGTGATTGA CCCCACCATC 
AACCCCCGGC ATATGGAGAT GTACGCTGAC CGAGAAAGCA GGGGATCAGT TCTGGAGCCA 
GAAGGGACAG TAGAAATCAA ATTCCGCAGA AAGGATCTGG TGAAAACCAT GCGTCGGGTG 
GACCCAGTCT ACATTCGCTT GGCTGAGCGA TTGGGCACCC CAGAGCTCAG CGCAGCTGAG 
CGGAAGGAGC TGGAGAACAA GTTGAAGGAG CGGGAGGAAT TCCTAATTCC CATTTACCAT 
CAGGTAGCCG TGCAGTTTGC TGACTTGCAC GACACACCAG GCCGGATGCA GGAGAAGGGT 
GTTATTAATG ACATCCTGGA TTGGAAAACA TCCCGTACCT TTTTCTATTG GCGGCTGAGG 
CGTCTCCTGC TGGAGGACCT GGTCAAAAAG AAAATCCACA ATGCCAACCC AGAGCTGACC 
GATGGCCAGA TCCAGGCCAT GCTACGGCGC TGGTTTGTAG AAGTGGAAGG AACTGTGAAG 
GCCTACGTTT GGGACAATAA CAAGGACCTG GTGGAGTGGC TGGAGAAACA GCTGACCGAG 
GAAGATGGTG TTCGCTCAGT AATCGAGGAA AACATCAAAT ATATCAGCAG AGACTATGTC 
CTCAAGCAGA TCCGCAGCTT GGTACAGGCC AATCCCGAGG TTGCCATGGA CTCCATCGTC 
CATATGACTC AGCATATCTC ACCCACTCAG CGAGCAGAAG TCGTACGGAT CCTCTCCACG 
ATGGACTCCC CTTCAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_005624778.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624835.1 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X7, mRNA.

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CloneID	OCe12093
Clone ID Related Accession (Same CDS sequence)	XM_005624780.3 , XM_005624780.2 , XM_005624783.2 , XM_005624778.2 , XM_005624779.3 , XM_005624779.2 , XM_005624783.3 , XM_005624778.3
Accession Version	XM_005624778.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	acetyl-CoA carboxylase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624778.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAC CATCCCCCTT GGCCAAACCT CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGTTCTG TGTCTGAAGA TAACTCAGAG GATGAAATTA GCAACCTGGT GAAGCTGGAT 
CTCCTGGAGG AGAAGGAGGG CTCTCTATCA CCAGCTTCTG TTGGCTCAGA TACACTTTCT 
GACTTGGGGA TCTCCAGCCT ACAGGACGGC TTGGCCTTAC ACATGAGGTC CAGCATGTCT 
GGCTTGCATC TAGTGAAGCA AGGTCGAGAC CGAAAGAAAA TAGACTCTCA GCGAGATTTC 
ACTGTAGCTT CTCCAGCAGA ATTTGTTACT CGTTTTGGGG GAAATAAAGT GATTGAGAAG 
GTTCTTATTG CCAACAATGG CATTGCAGCA GTGAAATGCA TGCGGTCTAT CCGTCGATGG 
TCTTATGAGA TGTTTCGAAA TGAACGTGCA ATCCGATTTG TTGTTATGGT AACACCTGAA 
GACCTGAAAG CCAATGCAGA ATACATTAAG ATGGCGGATC ACTATGTGCC AGTGCCAGGA 
GGACCAAATA ACAACAACTA TGCGAACGTG GAATTAATTC TTGATATTGC TAAAAGGATT 
CCCGTACAAG CAGTGTGGGC TGGCTGGGGT CATGCTTCTG AGAATCCCAA GCTCCCAGAA 
CTTCTTTTGA AAAATGGCAT CGCCTTCATG GGCCCTCCAA GCCAGGCCAT GTGGGCTTTG 
GGGGATAAGA TTGCATCTTC TATAGTGGCT CAAACTGCAG GTATCCCAAC TCTTCCCTGG 
AGTGGCAGTG GTCTTCGGGT GGACTGGCAT GAAAATGATT TTTCCAAACG TATCTTAAAT 
GTTCCCCAGG AATTATACGA AAAAGGTTAT GTGAAGGATG TGGATGATGG GCTAAAGGCA 
GCGGAGGAAG TTGGATATCC AGTAATGATC AAGGCCTCAG AAGGAGGAGG AGGGAAAGGA 
ATCAGAAAAG TCAACAATGC AGATGATTTC CCTAACCTCT TCAGACAGGT TCAAGCTGAA 
GTCCCTGGAT CTCCTATATT TGTGATGAGA CTAGCCAAAC AATCTCGTCA TCTGGAGGTA 
CAGATCTTAG CAGATCAATA TGGCAATGCT ATCTCTCTCT TTGGTCGTGA CTGCTCTGTA 
CAGCGCAGGC ATCAGAAGAT TATTGAGGAA GCACCTGCTG CTATTGCTAC TCCAGCAGTA 
TTTGAACATA TGGAACAGTG TGCTGTGAAA CTTGCCAAAA TGGTTGGTTA TGTGAGTGCT 
GGGACTGTGG AATACCTCTA CAGCCAGGAT GGAAGCTTCT ACTTTCTGGA ACTGAACCCT 
CGGCTACAGG TGGAACACCC TTGTACAGAG ATGGTGGCTG ATGTCAACCT CCCTGCAGCA 
CAACTCCAGA TTGCCATGGG GATCCCTCTG TACAGAATCA AGGATATTCG TATGATGTAT 
GGGGTATCTC CCTGGGGTGA TGCTCCCATT GATTTTGAAA ATTCTGCTCA CGTTCCTTGC 
CCAAGGGGCC ATGTTATTGC CGCTCGGATC ACTAGTGAAA ATCCAGATGA GGGTTTTAAG 
CCCAGCTCAG GAACAGTTCA GGAACTGAAT TTCCGTAGCA ATAAGAATGT TTGGGGTTAT 
TTCAGTGTTG CTGCTGCAGG GGGCCTTCAT GAATTTGCTG ATTCTCAGTT TGGTCATTGC 
TTCTCCTGGG GAGAAAACAG AGAGGAAGCA ATTTCAAACA TGGTGGTGGC TTTGAAGGAG 
CTGTCTATTC GGGGTGACTT TCGAACCACA GTTGAATACC TCATCAAATT GTTGGAGACT 
GAAAGCTTTC AGTTGAATAG AATTGACACC GGCTGGCTGG ACAGACTGAT AGCAGAGAAA 
GTACAGGCAG AGCGACCTGA CACCATGTTG GGAGTTGTTT GTGGGGCTCT CCATGTGGCA 
GATGTGAGCC TACGGAATAG CATCTCTAAC TTCCTTCATT CTTTAGAAAG GGGTCAAGTC 
CTTCCTGCTC ATACACTTCT GAATACAGTA GATGTTGAAC TTATCTATGG GGGAGTCAAA 
TATGTACTTA AGGTGACTCG GCAGTCCCCC AACTCCTATG TGGTGATCAT GAATGGTTCA 
TGCGTAGAAG TAGATGTGCA TCGGCTCAGT GATGGAGGGT TACTCCTGTC CTATGATGGC 
AGCAGTTACA CCACATACAT GAAGGAAGAA GTGGATAGAT ATCGTATCAC AATTGGCAAT 
AAGACCTGTG TGTTTGAGAA GGAAAATGAC CCTTCAGTGT TACGCTCACC TTCTGCTGGG 
AAGTTAATCC AGTACATTGT GGAGGATGGA GGCCATGTGT TTGCTGGCCA GTGCTATGCT 
GAAATTGAGG TGATGAAGAT GGTAATGACC TTAACAGCTG CAGAGTCTGG CTGTATCCAT 
TATGTCAAAC GGCCTGGAGC AGCTCTTGAC CCTGGCTGTG TAATAGCCAA AATGCAGTTG 
GACAACCCCA GCAAGGTCCA ACAGGCTGAG CTTCACACAG GTAGTCTGCC ACGGATCCAG 
AGCACTGCAC TCAGAGGCGA GAAGCTCCAT CGAGTGTTCC ACTATGTCCT GGATAATCTG 
GTCAACGTAA TGAATGGATA CTGCCTTCCA GATCCTTTTT TTAGCAGCAG GGTAAAAGAC 
TGGGTAGAGC GGTTGATGAA GACCCTCAGA GACCCCTCGT TACCTCTCCT AGAATTACAA 
GATATCATGA CCAGTGTCTC TGGCCGTATT CCCCCCAATG TTGAGAAGTC TATCAAGAAG 
GAAATGGCCC AGTATGCCAG CAATATCACA TCAGTCCTCT GTCAGTTTCC CAGCCAGCAG 
ATTGCCAACA TCCTAGATAG CCATGCAGCT ACGTTGAACC GGAAATCTGA GCGGGAAGTC 
TTTTTCATGA ACACTCAGAG CATTGTCCAG CTGGTACAGA GATACCGAAG TGGCATCCGA 
GGCCACATGA AGGCTGTGGT GATGGATCTG CTGCGGCAGT ACCTGCGAGT AGAGACACAG 
TTCCAGAATG GTCACTATGA CAAATGTGTG TTTGCCCTTC GAGAAGAGAA CAAGAGTGAT 
ATGAACACTG TACTGAACTA TATCTTCTCT CATGCTCAAG TCACCAAGAA GAATCTTCTG 
GTCACAATGC TTATTGATCA GTTGTGCGGT AGGGACCCTA CTCTCACTGA TGAGCTGCTG 
AATATTCTCA CAGAGCTAAC TCAACTCAGT AAGACCACCA ATGCCAAAGT AGCACTTCGA 
GCACGCCAGG TTCTTATTGC CTCCCATTTG CCATCATATG AGCTTCGCCA TAACCAAGTA 
GAGTCTATCT TCCTTTCAGC TATTGACATG TATGGACATC AGTTTTGCAT TGAGAACCTG 
CAGAAACTCA TCTTGTCTGA AACATCTATT TTTGATGTCC TACCAAACTT CTTCTATCAC 
AGCAACCAGG TAGTGAGGAT GGCAGCTCTG GAGGTTTATG TTCGAAGGGC TTATATTGCC 
TATGAACTTA ACAGCGTACA ACATCGCCAA CTTAAGGACA ACACCTGTGT GGTGGAATTC 
CAATTCATGC TACCCACATC TCATCCAAAT AGAGGGAACA TCCCCACGCT AAACAGAATG 
TCCTTCTCCT CCAACCTCAA CCACTATGGC ATGACTCATG TAGCTAGTGT CAGCGATGTG 
CTTTTGGACA ACTCATTCAC TCCACCATGT CAACGGATGG GCGGAATGGT CTCTTTTCGG 
ACTTTCGAAG ATTTTGTCAG GATCTTTGAT GAAGTGATGG GCTGCTTCTG TGATTCTCCA 
CCGCAAAGCC CTACATTCCC TGAGGCAGGT CACACATCTC TATACGATGA AGACAAGGTC 
CCCAGGGATG AACCAATTCA CATTCTGAAT GTGGCTATCA AGACTGACTG TGATATTGAG 
GATGACAGAC TGGCAGCTAT GTTCAGAGAG TTTACCCAAC AAAATAAAGC TACCCTGGTT 
GAGCATGGGA TACGGCGCCT TACTTTCCTG GTTGCACAAA AGGATTTCAG GAAACAGGTC 
AACTATGAGG TGGATCAGAG ATTTCATAGA GAATTTCCTA AATTTTTCAC CTTCCGAGCA 
AGGGATAAGT TTGAGGAGGA TCGTATCTAT CGTCACCTGG AACCTGCCCT CGCTTTCCAG 
TTAGAGCTGA ACCGGATGAG AAATTTTGAC CTTACTGCCA TTCCATGTGC TAATCATAAA 
ATGCATCTGT ATCTTGGGGC AGCCAAGGTG GAGGTGGGCA CAGAAGTGAC AGACTACAGG 
TTCTTTGTTC GTGCAATCAT CAGACATTCT GATCTGGTCA CCAAGGAAGC TTCTTTTGAA 
TACCTACAAA ATGAAGGGGA GAGGCTGCTT CTAGAAGCCA TGGATGAGTT GGAAGTTGCT 
TTTAACAATA CAAACGTTCG CACTGACTGT AACCACATCT TCCTCAACTT TGTGCCCACG 
GTCATCATGG ACCCATCAAA GATTGAGGAA TCTGTGCGGA GCATGGTAAT GCGCTATGGA 
AGTCGGCTGT GGAAATTACG TGTCCTCCAG GCAGAACTGA AAATCAACAT TCGCCTGACA 
CCAACTGGAA AAGCAATTCC CATCCGCCTC TTCCTGACAA ACGAGTCTGG CTATTACTTG 
GACATCAGCT TGTATAAGGA AGTGACTGAC TCCAGGACAG CACAGATCAT GTTTCAGGCA 
TATGGAGACA AACAGGGACC ATTGCATGGA ATGTTAATCA ACACTCCATA CGTCACCAAA 
GACCTGCTTC AATCAAAGAG ATTCCAGGCA CAATCCTTGG GGACAACATA CATATATGAT 
ATACCAGAGA TGTTTCGGCA GTCTCTGATC AAACTCTGGG AGTCTATGTC CACTCAAGCA 
TTTCTTCCAT CACCCCCCCT GCCTTCCGAC ATGCTGACAT ATACGGAGCT CGTGTTGGAT 
GATCAAGGTC AGCTGGTCCA CATGAACAGG CTTCCAGGAG GAAATGAGAT TGGCATGGTA 
GCGTGGAAAA TGACCCTTAA AAGTCCTGAA TACCCAGAAG GCCGAGATAT CATTGTTATT 
GGCAATGACA TCACATACCG AATTGGGTCC TTTGGGCCCC AGGAGGATTT GCTGTTTCTC 
AGAGCTTCTG AGCTTGCCAG GGCAGAGGGT ATCCCACGCA TCTTTGTAGC AGCCAACAGT 
GGAGCAAGAA TTGGACTGGC AGAGGAAATT CGTCATATGT TTCATGTAGC CTGGGTAGAT 
CCTGAGGATC CTTACAAGGG ATACAAATAT TTATATCTGA CCCCTCAGGA TTATAAGAGA 
GTCAGTGCTC TCAACTCTGT CCATTGTGAA CATGTAGAGG ACGAAGGAGA ATCCAGGTAC 
AAGATAACTG ATATTATTGG GAAGGAAGAG GGACTTGGAG CAGAGAACCT TCGAGGCTCT 
GGAATGATCG CTGGAGAATC CTCATTGGCC TATGATGAGA TCATCACCAT CAGCCTGGTT 
ACATGCCGGG CCATTGGGAT TGGAGCTTAC CTTGTCCGGC TGGGACAGAG AACCATCCAG 
GTTGAAAATT CTCATTTAAT TTTGACTGGA GCTGGAGCCC TCAACAAAGT CCTTGGGCGG 
GAAGTATACA CCTCCAATAA CCAGCTCGGG GGCATCCAGA TCATGCACAA CAATGGGGTG 
ACCCACAGCA TTGTTTGTGA TGACTTTGAA GGGGTTTTCA CTGTCCTGCA CTGGCTATCC 
TACATGCCTA AGAATGTGCG CAGTTTAGTT CCTCTCCTGA ACTCCAAGGA TCCTATAGAT 
AGAATCATCG AGTTTGTTCC CACGAAGGCC CCATACGATC CTCGATGGAT GCTAGCAGGC 
CGCCCTCACC CAACTCAGAA AGGTCAGTGG CTGAGTGGAT TTTTTGACTA TGGCTCTTTC 
TCAGAGATCA TGCAACCCTG GGCACAAACT GTGGTGGTTG GCAGAGCCAG GCTAGGAGGA 
ATACCTGTGG GAGTAGTTGC TGTAGAAACC CGAACAGTGG AACTAAGTAT CCCAGCCGAT 
CCTGCAAACC TGGATTCTGA AGCCAAGATA ATCCAGCAGG CTGGCCAGGT TTGGTTCCCA 
GACTCTGCGT TTAAGACCTA TCAGGCTATC AAGGACTTCA ACCGTGAAGG ACTGCCTCTG 
ATGGTCTTTG CCAACTGGAG AGGCTTCTCT GGTGGGATGA AAGATATGTA TGACCAAGTG 
CTGAAGTTTG GTGCCTATAT TGTGGATAGC TTACGGGAGT GCTCCCAGCC TGTGCTGGTA 
TACATTCCTC CTCAGGCTGA GCTCCGGGGT GGCTCCTGGG TTGTGATTGA CCCCACCATC 
AACCCCCGGC ATATGGAGAT GTACGCTGAC CGAGAAAGCA GGGGATCAGT TCTGGAGCCA 
GAAGGGACAG TAGAAATCAA ATTCCGCAGA AAGGATCTGG TGAAAACCAT GCGTCGGGTG 
GACCCAGTCT ACATTCGCTT GGCTGAGCGA TTGGGCACCC CAGAGCTCAG CGCAGCTGAG 
CGGAAGGAGC TGGAGAACAA GTTGAAGGAG CGGGAGGAAT TCCTAATTCC CATTTACCAT 
CAGGTAGCCG TGCAGTTTGC TGACTTGCAC GACACACCAG GCCGGATGCA GGAGAAGGGT 
GTTATTAATG ACATCCTGGA TTGGAAAACA TCCCGTACCT TTTTCTATTG GCGGCTGAGG 
CGTCTCCTGC TGGAGGACCT GGTCAAAAAG AAAATCCACA ATGCCAACCC AGAGCTGACC 
GATGGCCAGA TCCAGGCCAT GCTACGGCGC TGGTTTGTAG AAGTGGAAGG AACTGTGAAG 
GCCTACGTTT GGGACAATAA CAAGGACCTG GTGGAGTGGC TGGAGAAACA GCTGACCGAG 
GAAGATGGTG TTCGCTCAGT AATCGAGGAA AACATCAAAT ATATCAGCAG AGACTATGTC 
CTCAAGCAGA TCCGCAGCTT GGTACAGGCC AATCCCGAGG TTGCCATGGA CTCCATCGTC 
CATATGACTC AGCATATCTC ACCCACTCAG CGAGCAGAAG TCGTACGGAT CCTCTCCACG 
ATGGACTCCC CTTCAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624835.1
CDS	155..7195
Translation	MDEPSPLAKPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDGLALHMRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_005624778.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624778.3

ORF Insert Sequence:

ATGGATGAAC CATCCCCCTT GGCCAAACCT CTGGAGCTGA ACCAGCACTC CCGATTCATA 
ATTGGTTCTG TGTCTGAAGA TAACTCAGAG GATGAAATTA GCAACCTGGT GAAGCTGGAT 
CTCCTGGAGG AGAAGGAGGG CTCTCTATCA CCAGCTTCTG TTGGCTCAGA TACACTTTCT 
GACTTGGGGA TCTCCAGCCT ACAGGACGGC TTGGCCTTAC ACATGAGGTC CAGCATGTCT 
GGCTTGCATC TAGTGAAGCA AGGTCGAGAC CGAAAGAAAA TAGACTCTCA GCGAGATTTC 
ACTGTAGCTT CTCCAGCAGA ATTTGTTACT CGTTTTGGGG GAAATAAAGT GATTGAGAAG 
GTTCTTATTG CCAACAATGG CATTGCAGCA GTGAAATGCA TGCGGTCTAT CCGTCGATGG 
TCTTATGAGA TGTTTCGAAA TGAACGTGCA ATCCGATTTG TTGTTATGGT AACACCTGAA 
GACCTGAAAG CCAATGCAGA ATACATTAAG ATGGCGGATC ACTATGTGCC AGTGCCAGGA 
GGACCAAATA ACAACAACTA TGCGAACGTG GAATTAATTC TTGATATTGC TAAAAGGATT 
CCCGTACAAG CAGTGTGGGC TGGCTGGGGT CATGCTTCTG AGAATCCCAA GCTCCCAGAA 
CTTCTTTTGA AAAATGGCAT CGCCTTCATG GGCCCTCCAA GCCAGGCCAT GTGGGCTTTG 
GGGGATAAGA TTGCATCTTC TATAGTGGCT CAAACTGCAG GTATCCCAAC TCTTCCCTGG 
AGTGGCAGTG GTCTTCGGGT GGACTGGCAT GAAAATGATT TTTCCAAACG TATCTTAAAT 
GTTCCCCAGG AATTATACGA AAAAGGTTAT GTGAAGGATG TGGATGATGG GCTAAAGGCA 
GCGGAGGAAG TTGGATATCC AGTAATGATC AAGGCCTCAG AAGGAGGAGG AGGGAAAGGA 
ATCAGAAAAG TCAACAATGC AGATGATTTC CCTAACCTCT TCAGACAGGT TCAAGCTGAA 
GTCCCTGGAT CTCCTATATT TGTGATGAGA CTAGCCAAAC AATCTCGTCA TCTGGAGGTA 
CAGATCTTAG CAGATCAATA TGGCAATGCT ATCTCTCTCT TTGGTCGTGA CTGCTCTGTA 
CAGCGCAGGC ATCAGAAGAT TATTGAGGAA GCACCTGCTG CTATTGCTAC TCCAGCAGTA 
TTTGAACATA TGGAACAGTG TGCTGTGAAA CTTGCCAAAA TGGTTGGTTA TGTGAGTGCT 
GGGACTGTGG AATACCTCTA CAGCCAGGAT GGAAGCTTCT ACTTTCTGGA ACTGAACCCT 
CGGCTACAGG TGGAACACCC TTGTACAGAG ATGGTGGCTG ATGTCAACCT CCCTGCAGCA 
CAACTCCAGA TTGCCATGGG GATCCCTCTG TACAGAATCA AGGATATTCG TATGATGTAT 
GGGGTATCTC CCTGGGGTGA TGCTCCCATT GATTTTGAAA ATTCTGCTCA CGTTCCTTGC 
CCAAGGGGCC ATGTTATTGC CGCTCGGATC ACTAGTGAAA ATCCAGATGA GGGTTTTAAG 
CCCAGCTCAG GAACAGTTCA GGAACTGAAT TTCCGTAGCA ATAAGAATGT TTGGGGTTAT 
TTCAGTGTTG CTGCTGCAGG GGGCCTTCAT GAATTTGCTG ATTCTCAGTT TGGTCATTGC 
TTCTCCTGGG GAGAAAACAG AGAGGAAGCA ATTTCAAACA TGGTGGTGGC TTTGAAGGAG 
CTGTCTATTC GGGGTGACTT TCGAACCACA GTTGAATACC TCATCAAATT GTTGGAGACT 
GAAAGCTTTC AGTTGAATAG AATTGACACC GGCTGGCTGG ACAGACTGAT AGCAGAGAAA 
GTACAGGCAG AGCGACCTGA CACCATGTTG GGAGTTGTTT GTGGGGCTCT CCATGTGGCA 
GATGTGAGCC TACGGAATAG CATCTCTAAC TTCCTTCATT CTTTAGAAAG GGGTCAAGTC 
CTTCCTGCTC ATACACTTCT GAATACAGTA GATGTTGAAC TTATCTATGG GGGAGTCAAA 
TATGTACTTA AGGTGACTCG GCAGTCCCCC AACTCCTATG TGGTGATCAT GAATGGTTCA 
TGCGTAGAAG TAGATGTGCA TCGGCTCAGT GATGGAGGGT TACTCCTGTC CTATGATGGC 
AGCAGTTACA CCACATACAT GAAGGAAGAA GTGGATAGAT ATCGTATCAC AATTGGCAAT 
AAGACCTGTG TGTTTGAGAA GGAAAATGAC CCTTCAGTGT TACGCTCACC TTCTGCTGGG 
AAGTTAATCC AGTACATTGT GGAGGATGGA GGCCATGTGT TTGCTGGCCA GTGCTATGCT 
GAAATTGAGG TGATGAAGAT GGTAATGACC TTAACAGCTG CAGAGTCTGG CTGTATCCAT 
TATGTCAAAC GGCCTGGAGC AGCTCTTGAC CCTGGCTGTG TAATAGCCAA AATGCAGTTG 
GACAACCCCA GCAAGGTCCA ACAGGCTGAG CTTCACACAG GTAGTCTGCC ACGGATCCAG 
AGCACTGCAC TCAGAGGCGA GAAGCTCCAT CGAGTGTTCC ACTATGTCCT GGATAATCTG 
GTCAACGTAA TGAATGGATA CTGCCTTCCA GATCCTTTTT TTAGCAGCAG GGTAAAAGAC 
TGGGTAGAGC GGTTGATGAA GACCCTCAGA GACCCCTCGT TACCTCTCCT AGAATTACAA 
GATATCATGA CCAGTGTCTC TGGCCGTATT CCCCCCAATG TTGAGAAGTC TATCAAGAAG 
GAAATGGCCC AGTATGCCAG CAATATCACA TCAGTCCTCT GTCAGTTTCC CAGCCAGCAG 
ATTGCCAACA TCCTAGATAG CCATGCAGCT ACGTTGAACC GGAAATCTGA GCGGGAAGTC 
TTTTTCATGA ACACTCAGAG CATTGTCCAG CTGGTACAGA GATACCGAAG TGGCATCCGA 
GGCCACATGA AGGCTGTGGT GATGGATCTG CTGCGGCAGT ACCTGCGAGT AGAGACACAG 
TTCCAGAATG GTCACTATGA CAAATGTGTG TTTGCCCTTC GAGAAGAGAA CAAGAGTGAT 
ATGAACACTG TACTGAACTA TATCTTCTCT CATGCTCAAG TCACCAAGAA GAATCTTCTG 
GTCACAATGC TTATTGATCA GTTGTGCGGT AGGGACCCTA CTCTCACTGA TGAGCTGCTG 
AATATTCTCA CAGAGCTAAC TCAACTCAGT AAGACCACCA ATGCCAAAGT AGCACTTCGA 
GCACGCCAGG TTCTTATTGC CTCCCATTTG CCATCATATG AGCTTCGCCA TAACCAAGTA 
GAGTCTATCT TCCTTTCAGC TATTGACATG TATGGACATC AGTTTTGCAT TGAGAACCTG 
CAGAAACTCA TCTTGTCTGA AACATCTATT TTTGATGTCC TACCAAACTT CTTCTATCAC 
AGCAACCAGG TAGTGAGGAT GGCAGCTCTG GAGGTTTATG TTCGAAGGGC TTATATTGCC 
TATGAACTTA ACAGCGTACA ACATCGCCAA CTTAAGGACA ACACCTGTGT GGTGGAATTC 
CAATTCATGC TACCCACATC TCATCCAAAT AGAGGGAACA TCCCCACGCT AAACAGAATG 
TCCTTCTCCT CCAACCTCAA CCACTATGGC ATGACTCATG TAGCTAGTGT CAGCGATGTG 
CTTTTGGACA ACTCATTCAC TCCACCATGT CAACGGATGG GCGGAATGGT CTCTTTTCGG 
ACTTTCGAAG ATTTTGTCAG GATCTTTGAT GAAGTGATGG GCTGCTTCTG TGATTCTCCA 
CCGCAAAGCC CTACATTCCC TGAGGCAGGT CACACATCTC TATACGATGA AGACAAGGTC 
CCCAGGGATG AACCAATTCA CATTCTGAAT GTGGCTATCA AGACTGACTG TGATATTGAG 
GATGACAGAC TGGCAGCTAT GTTCAGAGAG TTTACCCAAC AAAATAAAGC TACCCTGGTT 
GAGCATGGGA TACGGCGCCT TACTTTCCTG GTTGCACAAA AGGATTTCAG GAAACAGGTC 
AACTATGAGG TGGATCAGAG ATTTCATAGA GAATTTCCTA AATTTTTCAC CTTCCGAGCA 
AGGGATAAGT TTGAGGAGGA TCGTATCTAT CGTCACCTGG AACCTGCCCT CGCTTTCCAG 
TTAGAGCTGA ACCGGATGAG AAATTTTGAC CTTACTGCCA TTCCATGTGC TAATCATAAA 
ATGCATCTGT ATCTTGGGGC AGCCAAGGTG GAGGTGGGCA CAGAAGTGAC AGACTACAGG 
TTCTTTGTTC GTGCAATCAT CAGACATTCT GATCTGGTCA CCAAGGAAGC TTCTTTTGAA 
TACCTACAAA ATGAAGGGGA GAGGCTGCTT CTAGAAGCCA TGGATGAGTT GGAAGTTGCT 
TTTAACAATA CAAACGTTCG CACTGACTGT AACCACATCT TCCTCAACTT TGTGCCCACG 
GTCATCATGG ACCCATCAAA GATTGAGGAA TCTGTGCGGA GCATGGTAAT GCGCTATGGA 
AGTCGGCTGT GGAAATTACG TGTCCTCCAG GCAGAACTGA AAATCAACAT TCGCCTGACA 
CCAACTGGAA AAGCAATTCC CATCCGCCTC TTCCTGACAA ACGAGTCTGG CTATTACTTG 
GACATCAGCT TGTATAAGGA AGTGACTGAC TCCAGGACAG CACAGATCAT GTTTCAGGCA 
TATGGAGACA AACAGGGACC ATTGCATGGA ATGTTAATCA ACACTCCATA CGTCACCAAA 
GACCTGCTTC AATCAAAGAG ATTCCAGGCA CAATCCTTGG GGACAACATA CATATATGAT 
ATACCAGAGA TGTTTCGGCA GTCTCTGATC AAACTCTGGG AGTCTATGTC CACTCAAGCA 
TTTCTTCCAT CACCCCCCCT GCCTTCCGAC ATGCTGACAT ATACGGAGCT CGTGTTGGAT 
GATCAAGGTC AGCTGGTCCA CATGAACAGG CTTCCAGGAG GAAATGAGAT TGGCATGGTA 
GCGTGGAAAA TGACCCTTAA AAGTCCTGAA TACCCAGAAG GCCGAGATAT CATTGTTATT 
GGCAATGACA TCACATACCG AATTGGGTCC TTTGGGCCCC AGGAGGATTT GCTGTTTCTC 
AGAGCTTCTG AGCTTGCCAG GGCAGAGGGT ATCCCACGCA TCTTTGTAGC AGCCAACAGT 
GGAGCAAGAA TTGGACTGGC AGAGGAAATT CGTCATATGT TTCATGTAGC CTGGGTAGAT 
CCTGAGGATC CTTACAAGGG ATACAAATAT TTATATCTGA CCCCTCAGGA TTATAAGAGA 
GTCAGTGCTC TCAACTCTGT CCATTGTGAA CATGTAGAGG ACGAAGGAGA ATCCAGGTAC 
AAGATAACTG ATATTATTGG GAAGGAAGAG GGACTTGGAG CAGAGAACCT TCGAGGCTCT 
GGAATGATCG CTGGAGAATC CTCATTGGCC TATGATGAGA TCATCACCAT CAGCCTGGTT 
ACATGCCGGG CCATTGGGAT TGGAGCTTAC CTTGTCCGGC TGGGACAGAG AACCATCCAG 
GTTGAAAATT CTCATTTAAT TTTGACTGGA GCTGGAGCCC TCAACAAAGT CCTTGGGCGG 
GAAGTATACA CCTCCAATAA CCAGCTCGGG GGCATCCAGA TCATGCACAA CAATGGGGTG 
ACCCACAGCA TTGTTTGTGA TGACTTTGAA GGGGTTTTCA CTGTCCTGCA CTGGCTATCC 
TACATGCCTA AGAATGTGCG CAGTTTAGTT CCTCTCCTGA ACTCCAAGGA TCCTATAGAT 
AGAATCATCG AGTTTGTTCC CACGAAGGCC CCATACGATC CTCGATGGAT GCTAGCAGGC 
CGCCCTCACC CAACTCAGAA AGGTCAGTGG CTGAGTGGAT TTTTTGACTA TGGCTCTTTC 
TCAGAGATCA TGCAACCCTG GGCACAAACT GTGGTGGTTG GCAGAGCCAG GCTAGGAGGA 
ATACCTGTGG GAGTAGTTGC TGTAGAAACC CGAACAGTGG AACTAAGTAT CCCAGCCGAT 
CCTGCAAACC TGGATTCTGA AGCCAAGATA ATCCAGCAGG CTGGCCAGGT TTGGTTCCCA 
GACTCTGCGT TTAAGACCTA TCAGGCTATC AAGGACTTCA ACCGTGAAGG ACTGCCTCTG 
ATGGTCTTTG CCAACTGGAG AGGCTTCTCT GGTGGGATGA AAGATATGTA TGACCAAGTG 
CTGAAGTTTG GTGCCTATAT TGTGGATAGC TTACGGGAGT GCTCCCAGCC TGTGCTGGTA 
TACATTCCTC CTCAGGCTGA GCTCCGGGGT GGCTCCTGGG TTGTGATTGA CCCCACCATC 
AACCCCCGGC ATATGGAGAT GTACGCTGAC CGAGAAAGCA GGGGATCAGT TCTGGAGCCA 
GAAGGGACAG TAGAAATCAA ATTCCGCAGA AAGGATCTGG TGAAAACCAT GCGTCGGGTG 
GACCCAGTCT ACATTCGCTT GGCTGAGCGA TTGGGCACCC CAGAGCTCAG CGCAGCTGAG 
CGGAAGGAGC TGGAGAACAA GTTGAAGGAG CGGGAGGAAT TCCTAATTCC CATTTACCAT 
CAGGTAGCCG TGCAGTTTGC TGACTTGCAC GACACACCAG GCCGGATGCA GGAGAAGGGT 
GTTATTAATG ACATCCTGGA TTGGAAAACA TCCCGTACCT TTTTCTATTG GCGGCTGAGG 
CGTCTCCTGC TGGAGGACCT GGTCAAAAAG AAAATCCACA ATGCCAACCC AGAGCTGACC 
GATGGCCAGA TCCAGGCCAT GCTACGGCGC TGGTTTGTAG AAGTGGAAGG AACTGTGAAG 
GCCTACGTTT GGGACAATAA CAAGGACCTG GTGGAGTGGC TGGAGAAACA GCTGACCGAG 
GAAGATGGTG TTCGCTCAGT AATCGAGGAA AACATCAAAT ATATCAGCAG AGACTATGTC 
CTCAAGCAGA TCCGCAGCTT GGTACAGGCC AATCCCGAGG TTGCCATGGA CTCCATCGTC 
CATATGACTC AGCATATCTC ACCCACTCAG CGAGCAGAAG TCGTACGGAT CCTCTCCACG 
ATGGACTCCC CTTCAACGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_548250.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_548250.3 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X8, mRNA.

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CloneID	OCe12094
Clone ID Related Accession (Same CDS sequence)	XM_548250.5 , XM_548250.6
Accession Version	XM_548250.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	acetyl-CoA carboxylase 1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_548250.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGTT CTGCAGAGGA AGTTAAGGAG ATGAGATATT ACATGCTTCA AAGGTCCAGC 
ATGTCTGGCT TGCATCTAGT GAAGCAAGGT CGAGACCGAA AGAAAATAGA CTCTCAGCGA 
GATTTCACTG TAGCTTCTCC AGCAGAATTT GTTACTCGTT TTGGGGGAAA TAAAGTGATT 
GAGAAGGTTC TTATTGCCAA CAATGGCATT GCAGCAGTGA AATGCATGCG GTCTATCCGT 
CGATGGTCTT ATGAGATGTT TCGAAATGAA CGTGCAATCC GATTTGTTGT TATGGTAACA 
CCTGAAGACC TGAAAGCCAA TGCAGAATAC ATTAAGATGG CGGATCACTA TGTGCCAGTG 
CCAGGAGGAC CAAATAACAA CAACTATGCG AACGTGGAAT TAATTCTTGA TATTGCTAAA 
AGGATTCCCG TACAAGCAGT GTGGGCTGGC TGGGGTCATG CTTCTGAGAA TCCCAAGCTC 
CCAGAACTTC TTTTGAAAAA TGGCATCGCC TTCATGGGCC CTCCAAGCCA GGCCATGTGG 
GCTTTGGGGG ATAAGATTGC ATCTTCTATA GTGGCTCAAA CTGCAGGTAT CCCAACTCTT 
CCCTGGAGTG GCAGTGGTCT TCGGGTGGAC TGGCATGAAA ATGATTTTTC CAAACGTATC 
TTAAATGTTC CCCAGGAATT ATACGAAAAA GGTTATGTGA AGGATGTGGA TGATGGGCTA 
AAGGCAGCGG AGGAAGTTGG ATATCCAGTA ATGATCAAGG CCTCAGAAGG AGGAGGAGGG 
AAAGGAATCA GAAAAGTCAA CAATGCAGAT GATTTCCCTA ACCTCTTCAG ACAGGTTCAA 
GCTGAAGTCC CTGGATCTCC TATATTTGTG ATGAGACTAG CCAAACAATC TCGTCATCTG 
GAGGTACAGA TCTTAGCAGA TCAATATGGC AATGCTATCT CTCTCTTTGG TCGTGACTGC 
TCTGTACAGC GCAGGCATCA GAAGATTATT GAGGAAGCAC CTGCTGCTAT TGCTACTCCA 
GCAGTATTTG AACATATGGA ACAGTGTGCT GTGAAACTTG CCAAAATGGT TGGTTATGTG 
AGTGCTGGGA CTGTGGAATA CCTCTACAGC CAGGATGGAA GCTTCTACTT TCTGGAACTG 
AACCCTCGGC TACAGGTGGA ACACCCTTGT ACAGAGATGG TGGCTGATGT CAACCTCCCT 
GCAGCACAAC TCCAGATTGC CATGGGGATC CCTCTGTACA GAATCAAGGA TATTCGTATG 
ATGTATGGGG TATCTCCCTG GGGTGATGCT CCCATTGATT TTGAAAATTC TGCTCACGTT 
CCTTGCCCAA GGGGCCATGT TATTGCCGCT CGGATCACTA GTGAAAATCC AGATGAGGGT 
TTTAAGCCCA GCTCAGGAAC AGTTCAGGAA CTGAATTTCC GTAGCAATAA GAATGTTTGG 
GGTTATTTCA GTGTTGCTGC TGCAGGGGGC CTTCATGAAT TTGCTGATTC TCAGTTTGGT 
CATTGCTTCT CCTGGGGAGA AAACAGAGAG GAAGCAATTT CAAACATGGT GGTGGCTTTG 
AAGGAGCTGT CTATTCGGGG TGACTTTCGA ACCACAGTTG AATACCTCAT CAAATTGTTG 
GAGACTGAAA GCTTTCAGTT GAATAGAATT GACACCGGCT GGCTGGACAG ACTGATAGCA 
GAGAAAGTAC AGGCAGAGCG ACCTGACACC ATGTTGGGAG TTGTTTGTGG GGCTCTCCAT 
GTGGCAGATG TGAGCCTACG GAATAGCATC TCTAACTTCC TTCATTCTTT AGAAAGGGGT 
CAAGTCCTTC CTGCTCATAC ACTTCTGAAT ACAGTAGATG TTGAACTTAT CTATGGGGGA 
GTCAAATATG TACTTAAGGT GACTCGGCAG TCCCCCAACT CCTATGTGGT GATCATGAAT 
GGTTCATGCG TAGAAGTAGA TGTGCATCGG CTCAGTGATG GAGGGTTACT CCTGTCCTAT 
GATGGCAGCA GTTACACCAC ATACATGAAG GAAGAAGTGG ATAGATATCG TATCACAATT 
GGCAATAAGA CCTGTGTGTT TGAGAAGGAA AATGACCCTT CAGTGTTACG CTCACCTTCT 
GCTGGGAAGT TAATCCAGTA CATTGTGGAG GATGGAGGCC ATGTGTTTGC TGGCCAGTGC 
TATGCTGAAA TTGAGGTGAT GAAGATGGTA ATGACCTTAA CAGCTGCAGA GTCTGGCTGT 
ATCCATTATG TCAAACGGCC TGGAGCAGCT CTTGACCCTG GCTGTGTAAT AGCCAAAATG 
CAGTTGGACA ACCCCAGCAA GGTCCAACAG GCTGAGCTTC ACACAGGTAG TCTGCCACGG 
ATCCAGAGCA CTGCACTCAG AGGCGAGAAG CTCCATCGAG TGTTCCACTA TGTCCTGGAT 
AATCTGGTCA ACGTAATGAA TGGATACTGC CTTCCAGATC CTTTTTTTAG CAGCAGGGTA 
AAAGACTGGG TAGAGCGGTT GATGAAGACC CTCAGAGACC CCTCGTTACC TCTCCTAGAA 
TTACAAGATA TCATGACCAG TGTCTCTGGC CGTATTCCCC CCAATGTTGA GAAGTCTATC 
AAGAAGGAAA TGGCCCAGTA TGCCAGCAAT ATCACATCAG TCCTCTGTCA GTTTCCCAGC 
CAGCAGATTG CCAACATCCT AGATAGCCAT GCAGCTACGT TGAACCGGAA ATCTGAGCGG 
GAAGTCTTTT TCATGAACAC TCAGAGCATT GTCCAGCTGG TACAGAGATA CCGAAGTGGC 
ATCCGAGGCC ACATGAAGGC TGTGGTGATG GATCTGCTGC GGCAGTACCT GCGAGTAGAG 
ACACAGTTCC AGAATGGTCA CTATGACAAA TGTGTGTTTG CCCTTCGAGA AGAGAACAAG 
AGTGATATGA ACACTGTACT GAACTATATC TTCTCTCATG CTCAAGTCAC CAAGAAGAAT 
CTTCTGGTCA CAATGCTTAT TGATCAGTTG TGCGGTAGGG ACCCTACTCT CACTGATGAG 
CTGCTGAATA TTCTCACAGA GCTAACTCAA CTCAGTAAGA CCACCAATGC CAAAGTAGCA 
CTTCGAGCAC GCCAGGTTCT TATTGCCTCC CATTTGCCAT CATATGAGCT TCGCCATAAC 
CAAGTAGAGT CTATCTTCCT TTCAGCTATT GACATGTATG GACATCAGTT TTGCATTGAG 
AACCTGCAGA AACTCATCTT GTCTGAAACA TCTATTTTTG ATGTCCTACC AAACTTCTTC 
TATCACAGCA ACCAGGTAGT GAGGATGGCA GCTCTGGAGG TTTATGTTCG AAGGGCTTAT 
ATTGCCTATG AACTTAACAG CGTACAACAT CGCCAACTTA AGGACAACAC CTGTGTGGTG 
GAATTCCAAT TCATGCTACC CACATCTCAT CCAAATAGAG GGAACATCCC CACGCTAAAC 
AGAATGTCCT TCTCCTCCAA CCTCAACCAC TATGGCATGA CTCATGTAGC TAGTGTCAGC 
GATGTGCTTT TGGACAACTC ATTCACTCCA CCATGTCAAC GGATGGGCGG AATGGTCTCT 
TTTCGGACTT TCGAAGATTT TGTCAGGATC TTTGATGAAG TGATGGGCTG CTTCTGTGAT 
TCTCCACCGC AAAGCCCTAC ATTCCCTGAG GCAGGTCACA CATCTCTATA CGATGAAGAC 
AAGGTCCCCA GGGATGAACC AATTCACATT CTGAATGTGG CTATCAAGAC TGACTGTGAT 
ATTGAGGATG ACAGACTGGC AGCTATGTTC AGAGAGTTTA CCCAACAAAA TAAAGCTACC 
CTGGTTGAGC ATGGGATACG GCGCCTTACT TTCCTGGTTG CACAAAAGGA TTTCAGGAAA 
CAGGTCAACT ATGAGGTGGA TCAGAGATTT CATAGAGAAT TTCCTAAATT TTTCACCTTC 
CGAGCAAGGG ATAAGTTTGA GGAGGATCGT ATCTATCGTC ACCTGGAACC TGCCCTCGCT 
TTCCAGTTAG AGCTGAACCG GATGAGAAAT TTTGACCTTA CTGCCATTCC ATGTGCTAAT 
CATAAAATGC ATCTGTATCT TGGGGCAGCC AAGGTGGAGG TGGGCACAGA AGTGACAGAC 
TACAGGTTCT TTGTTCGTGC AATCATCAGA CATTCTGATC TGGTCACCAA GGAAGCTTCT 
TTTGAATACC TACAAAATGA AGGGGAGAGG CTGCTTCTAG AAGCCATGGA TGAGTTGGAA 
GTTGCTTTTA ACAATACAAA CGTTCGCACT GACTGTAACC ACATCTTCCT CAACTTTGTG 
CCCACGGTCA TCATGGACCC ATCAAAGATT GAGGAATCTG TGCGGAGCAT GGTAATGCGC 
TATGGAAGTC GGCTGTGGAA ATTACGTGTC CTCCAGGCAG AACTGAAAAT CAACATTCGC 
CTGACACCAA CTGGAAAAGC AATTCCCATC CGCCTCTTCC TGACAAACGA GTCTGGCTAT 
TACTTGGACA TCAGCTTGTA TAAGGAAGTG ACTGACTCCA GGACAGCACA GATCATGTTT 
CAGGCATATG GAGACAAACA GGGACCATTG CATGGAATGT TAATCAACAC TCCATACGTC 
ACCAAAGACC TGCTTCAATC AAAGAGATTC CAGGCACAAT CCTTGGGGAC AACATACATA 
TATGATATAC CAGAGATGTT TCGGCAGTCT CTGATCAAAC TCTGGGAGTC TATGTCCACT 
CAAGCATTTC TTCCATCACC CCCCCTGCCT TCCGACATGC TGACATATAC GGAGCTCGTG 
TTGGATGATC AAGGTCAGCT GGTCCACATG AACAGGCTTC CAGGAGGAAA TGAGATTGGC 
ATGGTAGCGT GGAAAATGAC CCTTAAAAGT CCTGAATACC CAGAAGGCCG AGATATCATT 
GTTATTGGCA ATGACATCAC ATACCGAATT GGGTCCTTTG GGCCCCAGGA GGATTTGCTG 
TTTCTCAGAG CTTCTGAGCT TGCCAGGGCA GAGGGTATCC CACGCATCTT TGTAGCAGCC 
AACAGTGGAG CAAGAATTGG ACTGGCAGAG GAAATTCGTC ATATGTTTCA TGTAGCCTGG 
GTAGATCCTG AGGATCCTTA CAAGGGATAC AAATATTTAT ATCTGACCCC TCAGGATTAT 
AAGAGAGTCA GTGCTCTCAA CTCTGTCCAT TGTGAACATG TAGAGGACGA AGGAGAATCC 
AGGTACAAGA TAACTGATAT TATTGGGAAG GAAGAGGGAC TTGGAGCAGA GAACCTTCGA 
GGCTCTGGAA TGATCGCTGG AGAATCCTCA TTGGCCTATG ATGAGATCAT CACCATCAGC 
CTGGTTACAT GCCGGGCCAT TGGGATTGGA GCTTACCTTG TCCGGCTGGG ACAGAGAACC 
ATCCAGGTTG AAAATTCTCA TTTAATTTTG ACTGGAGCTG GAGCCCTCAA CAAAGTCCTT 
GGGCGGGAAG TATACACCTC CAATAACCAG CTCGGGGGCA TCCAGATCAT GCACAACAAT 
GGGGTGACCC ACAGCATTGT TTGTGATGAC TTTGAAGGGG TTTTCACTGT CCTGCACTGG 
CTATCCTACA TGCCTAAGAA TGTGCGCAGT TTAGTTCCTC TCCTGAACTC CAAGGATCCT 
ATAGATAGAA TCATCGAGTT TGTTCCCACG AAGGCCCCAT ACGATCCTCG ATGGATGCTA 
GCAGGCCGCC CTCACCCAAC TCAGAAAGGT CAGTGGCTGA GTGGATTTTT TGACTATGGC 
TCTTTCTCAG AGATCATGCA ACCCTGGGCA CAAACTGTGG TGGTTGGCAG AGCCAGGCTA 
GGAGGAATAC CTGTGGGAGT AGTTGCTGTA GAAACCCGAA CAGTGGAACT AAGTATCCCA 
GCCGATCCTG CAAACCTGGA TTCTGAAGCC AAGATAATCC AGCAGGCTGG CCAGGTTTGG 
TTCCCAGACT CTGCGTTTAA GACCTATCAG GCTATCAAGG ACTTCAACCG TGAAGGACTG 
CCTCTGATGG TCTTTGCCAA CTGGAGAGGC TTCTCTGGTG GGATGAAAGA TATGTATGAC 
CAAGTGCTGA AGTTTGGTGC CTATATTGTG GATAGCTTAC GGGAGTGCTC CCAGCCTGTG 
CTGGTATACA TTCCTCCTCA GGCTGAGCTC CGGGGTGGCT CCTGGGTTGT GATTGACCCC 
ACCATCAACC CCCGGCATAT GGAGATGTAC GCTGACCGAG AAAGCAGGGG ATCAGTTCTG 
GAGCCAGAAG GGACAGTAGA AATCAAATTC CGCAGAAAGG ATCTGGTGAA AACCATGCGT 
CGGGTGGACC CAGTCTACAT TCGCTTGGCT GAGCGATTGG GCACCCCAGA GCTCAGCGCA 
GCTGAGCGGA AGGAGCTGGA GAACAAGTTG AAGGAGCGGG AGGAATTCCT AATTCCCATT 
TACCATCAGG TAGCCGTGCA GTTTGCTGAC TTGCACGACA CACCAGGCCG GATGCAGGAG 
AAGGGTGTTA TTAATGACAT CCTGGATTGG AAAACATCCC GTACCTTTTT CTATTGGCGG 
CTGAGGCGTC TCCTGCTGGA GGACCTGGTC AAAAAGAAAA TCCACAATGC CAACCCAGAG 
CTGACCGATG GCCAGATCCA GGCCATGCTA CGGCGCTGGT TTGTAGAAGT GGAAGGAACT 
GTGAAGGCCT ACGTTTGGGA CAATAACAAG GACCTGGTGG AGTGGCTGGA GAAACAGCTG 
ACCGAGGAAG ATGGTGTTCG CTCAGTAATC GAGGAAAACA TCAAATATAT CAGCAGAGAC 
TATGTCCTCA AGCAGATCCG CAGCTTGGTA CAGGCCAATC CCGAGGTTGC CATGGACTCC 
ATCGTCCATA TGACTCAGCA TATCTCACCC ACTCAGCGAG CAGAAGTCGT ACGGATCCTC 
TCCACGATGG ACTCCCCTTC AACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_548250.3
CDS	1353..8219
Translation	MEGSAEEVKEMRYYMLQRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_548250.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_548250.5

ORF Insert Sequence:

ATGGAGGGTT CTGCAGAGGA AGTTAAGGAG ATGAGATATT ACATGCTTCA AAGGTCCAGC 
ATGTCTGGCT TGCATCTAGT GAAGCAAGGT CGAGACCGAA AGAAAATAGA CTCTCAGCGA 
GATTTCACTG TAGCTTCTCC AGCAGAATTT GTTACTCGTT TTGGGGGAAA TAAAGTGATT 
GAGAAGGTTC TTATTGCCAA CAATGGCATT GCAGCAGTGA AATGCATGCG GTCTATCCGT 
CGATGGTCTT ATGAGATGTT TCGAAATGAA CGTGCAATCC GATTTGTTGT TATGGTAACA 
CCTGAAGACC TGAAAGCCAA TGCAGAATAC ATTAAGATGG CGGATCACTA TGTGCCAGTG 
CCAGGAGGAC CAAATAACAA CAACTATGCG AACGTGGAAT TAATTCTTGA TATTGCTAAA 
AGGATTCCCG TACAAGCAGT GTGGGCTGGC TGGGGTCATG CTTCTGAGAA TCCCAAGCTC 
CCAGAACTTC TTTTGAAAAA TGGCATCGCC TTCATGGGCC CTCCAAGCCA GGCCATGTGG 
GCTTTGGGGG ATAAGATTGC ATCTTCTATA GTGGCTCAAA CTGCAGGTAT CCCAACTCTT 
CCCTGGAGTG GCAGTGGTCT TCGGGTGGAC TGGCATGAAA ATGATTTTTC CAAACGTATC 
TTAAATGTTC CCCAGGAATT ATACGAAAAA GGTTATGTGA AGGATGTGGA TGATGGGCTA 
AAGGCAGCGG AGGAAGTTGG ATATCCAGTA ATGATCAAGG CCTCAGAAGG AGGAGGAGGG 
AAAGGAATCA GAAAAGTCAA CAATGCAGAT GATTTCCCTA ACCTCTTCAG ACAGGTTCAA 
GCTGAAGTCC CTGGATCTCC TATATTTGTG ATGAGACTAG CCAAACAATC TCGTCATCTG 
GAGGTACAGA TCTTAGCAGA TCAATATGGC AATGCTATCT CTCTCTTTGG TCGTGACTGC 
TCTGTACAGC GCAGGCATCA GAAGATTATT GAGGAAGCAC CTGCTGCTAT TGCTACTCCA 
GCAGTATTTG AACATATGGA ACAGTGTGCT GTGAAACTTG CCAAAATGGT TGGTTATGTG 
AGTGCTGGGA CTGTGGAATA CCTCTACAGC CAGGATGGAA GCTTCTACTT TCTGGAACTG 
AACCCTCGGC TACAGGTGGA ACACCCTTGT ACAGAGATGG TGGCTGATGT CAACCTCCCT 
GCAGCACAAC TCCAGATTGC CATGGGGATC CCTCTGTACA GAATCAAGGA TATTCGTATG 
ATGTATGGGG TATCTCCCTG GGGTGATGCT CCCATTGATT TTGAAAATTC TGCTCACGTT 
CCTTGCCCAA GGGGCCATGT TATTGCCGCT CGGATCACTA GTGAAAATCC AGATGAGGGT 
TTTAAGCCCA GCTCAGGAAC AGTTCAGGAA CTGAATTTCC GTAGCAATAA GAATGTTTGG 
GGTTATTTCA GTGTTGCTGC TGCAGGGGGC CTTCATGAAT TTGCTGATTC TCAGTTTGGT 
CATTGCTTCT CCTGGGGAGA AAACAGAGAG GAAGCAATTT CAAACATGGT GGTGGCTTTG 
AAGGAGCTGT CTATTCGGGG TGACTTTCGA ACCACAGTTG AATACCTCAT CAAATTGTTG 
GAGACTGAAA GCTTTCAGTT GAATAGAATT GACACCGGCT GGCTGGACAG ACTGATAGCA 
GAGAAAGTAC AGGCAGAGCG ACCTGACACC ATGTTGGGAG TTGTTTGTGG GGCTCTCCAT 
GTGGCAGATG TGAGCCTACG GAATAGCATC TCTAACTTCC TTCATTCTTT AGAAAGGGGT 
CAAGTCCTTC CTGCTCATAC ACTTCTGAAT ACAGTAGATG TTGAACTTAT CTATGGGGGA 
GTCAAATATG TACTTAAGGT GACTCGGCAG TCCCCCAACT CCTATGTGGT GATCATGAAT 
GGTTCATGCG TAGAAGTAGA TGTGCATCGG CTCAGTGATG GAGGGTTACT CCTGTCCTAT 
GATGGCAGCA GTTACACCAC ATACATGAAG GAAGAAGTGG ATAGATATCG TATCACAATT 
GGCAATAAGA CCTGTGTGTT TGAGAAGGAA AATGACCCTT CAGTGTTACG CTCACCTTCT 
GCTGGGAAGT TAATCCAGTA CATTGTGGAG GATGGAGGCC ATGTGTTTGC TGGCCAGTGC 
TATGCTGAAA TTGAGGTGAT GAAGATGGTA ATGACCTTAA CAGCTGCAGA GTCTGGCTGT 
ATCCATTATG TCAAACGGCC TGGAGCAGCT CTTGACCCTG GCTGTGTAAT AGCCAAAATG 
CAGTTGGACA ACCCCAGCAA GGTCCAACAG GCTGAGCTTC ACACAGGTAG TCTGCCACGG 
ATCCAGAGCA CTGCACTCAG AGGCGAGAAG CTCCATCGAG TGTTCCACTA TGTCCTGGAT 
AATCTGGTCA ACGTAATGAA TGGATACTGC CTTCCAGATC CTTTTTTTAG CAGCAGGGTA 
AAAGACTGGG TAGAGCGGTT GATGAAGACC CTCAGAGACC CCTCGTTACC TCTCCTAGAA 
TTACAAGATA TCATGACCAG TGTCTCTGGC CGTATTCCCC CCAATGTTGA GAAGTCTATC 
AAGAAGGAAA TGGCCCAGTA TGCCAGCAAT ATCACATCAG TCCTCTGTCA GTTTCCCAGC 
CAGCAGATTG CCAACATCCT AGATAGCCAT GCAGCTACGT TGAACCGGAA ATCTGAGCGG 
GAAGTCTTTT TCATGAACAC TCAGAGCATT GTCCAGCTGG TACAGAGATA CCGAAGTGGC 
ATCCGAGGCC ACATGAAGGC TGTGGTGATG GATCTGCTGC GGCAGTACCT GCGAGTAGAG 
ACACAGTTCC AGAATGGTCA CTATGACAAA TGTGTGTTTG CCCTTCGAGA AGAGAACAAG 
AGTGATATGA ACACTGTACT GAACTATATC TTCTCTCATG CTCAAGTCAC CAAGAAGAAT 
CTTCTGGTCA CAATGCTTAT TGATCAGTTG TGCGGTAGGG ACCCTACTCT CACTGATGAG 
CTGCTGAATA TTCTCACAGA GCTAACTCAA CTCAGTAAGA CCACCAATGC CAAAGTAGCA 
CTTCGAGCAC GCCAGGTTCT TATTGCCTCC CATTTGCCAT CATATGAGCT TCGCCATAAC 
CAAGTAGAGT CTATCTTCCT TTCAGCTATT GACATGTATG GACATCAGTT TTGCATTGAG 
AACCTGCAGA AACTCATCTT GTCTGAAACA TCTATTTTTG ATGTCCTACC AAACTTCTTC 
TATCACAGCA ACCAGGTAGT GAGGATGGCA GCTCTGGAGG TTTATGTTCG AAGGGCTTAT 
ATTGCCTATG AACTTAACAG CGTACAACAT CGCCAACTTA AGGACAACAC CTGTGTGGTG 
GAATTCCAAT TCATGCTACC CACATCTCAT CCAAATAGAG GGAACATCCC CACGCTAAAC 
AGAATGTCCT TCTCCTCCAA CCTCAACCAC TATGGCATGA CTCATGTAGC TAGTGTCAGC 
GATGTGCTTT TGGACAACTC ATTCACTCCA CCATGTCAAC GGATGGGCGG AATGGTCTCT 
TTTCGGACTT TCGAAGATTT TGTCAGGATC TTTGATGAAG TGATGGGCTG CTTCTGTGAT 
TCTCCACCGC AAAGCCCTAC ATTCCCTGAG GCAGGTCACA CATCTCTATA CGATGAAGAC 
AAGGTCCCCA GGGATGAACC AATTCACATT CTGAATGTGG CTATCAAGAC TGACTGTGAT 
ATTGAGGATG ACAGACTGGC AGCTATGTTC AGAGAGTTTA CCCAACAAAA TAAAGCTACC 
CTGGTTGAGC ATGGGATACG GCGCCTTACT TTCCTGGTTG CACAAAAGGA TTTCAGGAAA 
CAGGTCAACT ATGAGGTGGA TCAGAGATTT CATAGAGAAT TTCCTAAATT TTTCACCTTC 
CGAGCAAGGG ATAAGTTTGA GGAGGATCGT ATCTATCGTC ACCTGGAACC TGCCCTCGCT 
TTCCAGTTAG AGCTGAACCG GATGAGAAAT TTTGACCTTA CTGCCATTCC ATGTGCTAAT 
CATAAAATGC ATCTGTATCT TGGGGCAGCC AAGGTGGAGG TGGGCACAGA AGTGACAGAC 
TACAGGTTCT TTGTTCGTGC AATCATCAGA CATTCTGATC TGGTCACCAA GGAAGCTTCT 
TTTGAATACC TACAAAATGA AGGGGAGAGG CTGCTTCTAG AAGCCATGGA TGAGTTGGAA 
GTTGCTTTTA ACAATACAAA CGTTCGCACT GACTGTAACC ACATCTTCCT CAACTTTGTG 
CCCACGGTCA TCATGGACCC ATCAAAGATT GAGGAATCTG TGCGGAGCAT GGTAATGCGC 
TATGGAAGTC GGCTGTGGAA ATTACGTGTC CTCCAGGCAG AACTGAAAAT CAACATTCGC 
CTGACACCAA CTGGAAAAGC AATTCCCATC CGCCTCTTCC TGACAAACGA GTCTGGCTAT 
TACTTGGACA TCAGCTTGTA TAAGGAAGTG ACTGACTCCA GGACAGCACA GATCATGTTT 
CAGGCATATG GAGACAAACA GGGACCATTG CATGGAATGT TAATCAACAC TCCATACGTC 
ACCAAAGACC TGCTTCAATC AAAGAGATTC CAGGCACAAT CCTTGGGGAC AACATACATA 
TATGATATAC CAGAGATGTT TCGGCAGTCT CTGATCAAAC TCTGGGAGTC TATGTCCACT 
CAAGCATTTC TTCCATCACC CCCCCTGCCT TCCGACATGC TGACATATAC GGAGCTCGTG 
TTGGATGATC AAGGTCAGCT GGTCCACATG AACAGGCTTC CAGGAGGAAA TGAGATTGGC 
ATGGTAGCGT GGAAAATGAC CCTTAAAAGT CCTGAATACC CAGAAGGCCG AGATATCATT 
GTTATTGGCA ATGACATCAC ATACCGAATT GGGTCCTTTG GGCCCCAGGA GGATTTGCTG 
TTTCTCAGAG CTTCTGAGCT TGCCAGGGCA GAGGGTATCC CACGCATCTT TGTAGCAGCC 
AACAGTGGAG CAAGAATTGG ACTGGCAGAG GAAATTCGTC ATATGTTTCA TGTAGCCTGG 
GTAGATCCTG AGGATCCTTA CAAGGGATAC AAATATTTAT ATCTGACCCC TCAGGATTAT 
AAGAGAGTCA GTGCTCTCAA CTCTGTCCAT TGTGAACATG TAGAGGACGA AGGAGAATCC 
AGGTACAAGA TAACTGATAT TATTGGGAAG GAAGAGGGAC TTGGAGCAGA GAACCTTCGA 
GGCTCTGGAA TGATCGCTGG AGAATCCTCA TTGGCCTATG ATGAGATCAT CACCATCAGC 
CTGGTTACAT GCCGGGCCAT TGGGATTGGA GCTTACCTTG TCCGGCTGGG ACAGAGAACC 
ATCCAGGTTG AAAATTCTCA TTTAATTTTG ACTGGAGCTG GAGCCCTCAA CAAAGTCCTT 
GGGCGGGAAG TATACACCTC CAATAACCAG CTCGGGGGCA TCCAGATCAT GCACAACAAT 
GGGGTGACCC ACAGCATTGT TTGTGATGAC TTTGAAGGGG TTTTCACTGT CCTGCACTGG 
CTATCCTACA TGCCTAAGAA TGTGCGCAGT TTAGTTCCTC TCCTGAACTC CAAGGATCCT 
ATAGATAGAA TCATCGAGTT TGTTCCCACG AAGGCCCCAT ACGATCCTCG ATGGATGCTA 
GCAGGCCGCC CTCACCCAAC TCAGAAAGGT CAGTGGCTGA GTGGATTTTT TGACTATGGC 
TCTTTCTCAG AGATCATGCA ACCCTGGGCA CAAACTGTGG TGGTTGGCAG AGCCAGGCTA 
GGAGGAATAC CTGTGGGAGT AGTTGCTGTA GAAACCCGAA CAGTGGAACT AAGTATCCCA 
GCCGATCCTG CAAACCTGGA TTCTGAAGCC AAGATAATCC AGCAGGCTGG CCAGGTTTGG 
TTCCCAGACT CTGCGTTTAA GACCTATCAG GCTATCAAGG ACTTCAACCG TGAAGGACTG 
CCTCTGATGG TCTTTGCCAA CTGGAGAGGC TTCTCTGGTG GGATGAAAGA TATGTATGAC 
CAAGTGCTGA AGTTTGGTGC CTATATTGTG GATAGCTTAC GGGAGTGCTC CCAGCCTGTG 
CTGGTATACA TTCCTCCTCA GGCTGAGCTC CGGGGTGGCT CCTGGGTTGT GATTGACCCC 
ACCATCAACC CCCGGCATAT GGAGATGTAC GCTGACCGAG AAAGCAGGGG ATCAGTTCTG 
GAGCCAGAAG GGACAGTAGA AATCAAATTC CGCAGAAAGG ATCTGGTGAA AACCATGCGT 
CGGGTGGACC CAGTCTACAT TCGCTTGGCT GAGCGATTGG GCACCCCAGA GCTCAGCGCA 
GCTGAGCGGA AGGAGCTGGA GAACAAGTTG AAGGAGCGGG AGGAATTCCT AATTCCCATT 
TACCATCAGG TAGCCGTGCA GTTTGCTGAC TTGCACGACA CACCAGGCCG GATGCAGGAG 
AAGGGTGTTA TTAATGACAT CCTGGATTGG AAAACATCCC GTACCTTTTT CTATTGGCGG 
CTGAGGCGTC TCCTGCTGGA GGACCTGGTC AAAAAGAAAA TCCACAATGC CAACCCAGAG 
CTGACCGATG GCCAGATCCA GGCCATGCTA CGGCGCTGGT TTGTAGAAGT GGAAGGAACT 
GTGAAGGCCT ACGTTTGGGA CAATAACAAG GACCTGGTGG AGTGGCTGGA GAAACAGCTG 
ACCGAGGAAG ATGGTGTTCG CTCAGTAATC GAGGAAAACA TCAAATATAT CAGCAGAGAC 
TATGTCCTCA AGCAGATCCG CAGCTTGGTA CAGGCCAATC CCGAGGTTGC CATGGACTCC 
ATCGTCCATA TGACTCAGCA TATCTCACCC ACTCAGCGAG CAGAAGTCGT ACGGATCCTC 
TCCACGATGG ACTCCCCTTC AACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_548250.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_548250.3 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X8, mRNA.

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CloneID	OCe12094
Clone ID Related Accession (Same CDS sequence)	XM_548250.5 , XM_548250.6
Accession Version	XM_548250.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	acetyl-CoA carboxylase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_548250.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGTT CTGCAGAGGA AGTTAAGGAG ATGAGATATT ACATGCTTCA AAGGTCCAGC 
ATGTCTGGCT TGCATCTAGT GAAGCAAGGT CGAGACCGAA AGAAAATAGA CTCTCAGCGA 
GATTTCACTG TAGCTTCTCC AGCAGAATTT GTTACTCGTT TTGGGGGAAA TAAAGTGATT 
GAGAAGGTTC TTATTGCCAA CAATGGCATT GCAGCAGTGA AATGCATGCG GTCTATCCGT 
CGATGGTCTT ATGAGATGTT TCGAAATGAA CGTGCAATCC GATTTGTTGT TATGGTAACA 
CCTGAAGACC TGAAAGCCAA TGCAGAATAC ATTAAGATGG CGGATCACTA TGTGCCAGTG 
CCAGGAGGAC CAAATAACAA CAACTATGCG AACGTGGAAT TAATTCTTGA TATTGCTAAA 
AGGATTCCCG TACAAGCAGT GTGGGCTGGC TGGGGTCATG CTTCTGAGAA TCCCAAGCTC 
CCAGAACTTC TTTTGAAAAA TGGCATCGCC TTCATGGGCC CTCCAAGCCA GGCCATGTGG 
GCTTTGGGGG ATAAGATTGC ATCTTCTATA GTGGCTCAAA CTGCAGGTAT CCCAACTCTT 
CCCTGGAGTG GCAGTGGTCT TCGGGTGGAC TGGCATGAAA ATGATTTTTC CAAACGTATC 
TTAAATGTTC CCCAGGAATT ATACGAAAAA GGTTATGTGA AGGATGTGGA TGATGGGCTA 
AAGGCAGCGG AGGAAGTTGG ATATCCAGTA ATGATCAAGG CCTCAGAAGG AGGAGGAGGG 
AAAGGAATCA GAAAAGTCAA CAATGCAGAT GATTTCCCTA ACCTCTTCAG ACAGGTTCAA 
GCTGAAGTCC CTGGATCTCC TATATTTGTG ATGAGACTAG CCAAACAATC TCGTCATCTG 
GAGGTACAGA TCTTAGCAGA TCAATATGGC AATGCTATCT CTCTCTTTGG TCGTGACTGC 
TCTGTACAGC GCAGGCATCA GAAGATTATT GAGGAAGCAC CTGCTGCTAT TGCTACTCCA 
GCAGTATTTG AACATATGGA ACAGTGTGCT GTGAAACTTG CCAAAATGGT TGGTTATGTG 
AGTGCTGGGA CTGTGGAATA CCTCTACAGC CAGGATGGAA GCTTCTACTT TCTGGAACTG 
AACCCTCGGC TACAGGTGGA ACACCCTTGT ACAGAGATGG TGGCTGATGT CAACCTCCCT 
GCAGCACAAC TCCAGATTGC CATGGGGATC CCTCTGTACA GAATCAAGGA TATTCGTATG 
ATGTATGGGG TATCTCCCTG GGGTGATGCT CCCATTGATT TTGAAAATTC TGCTCACGTT 
CCTTGCCCAA GGGGCCATGT TATTGCCGCT CGGATCACTA GTGAAAATCC AGATGAGGGT 
TTTAAGCCCA GCTCAGGAAC AGTTCAGGAA CTGAATTTCC GTAGCAATAA GAATGTTTGG 
GGTTATTTCA GTGTTGCTGC TGCAGGGGGC CTTCATGAAT TTGCTGATTC TCAGTTTGGT 
CATTGCTTCT CCTGGGGAGA AAACAGAGAG GAAGCAATTT CAAACATGGT GGTGGCTTTG 
AAGGAGCTGT CTATTCGGGG TGACTTTCGA ACCACAGTTG AATACCTCAT CAAATTGTTG 
GAGACTGAAA GCTTTCAGTT GAATAGAATT GACACCGGCT GGCTGGACAG ACTGATAGCA 
GAGAAAGTAC AGGCAGAGCG ACCTGACACC ATGTTGGGAG TTGTTTGTGG GGCTCTCCAT 
GTGGCAGATG TGAGCCTACG GAATAGCATC TCTAACTTCC TTCATTCTTT AGAAAGGGGT 
CAAGTCCTTC CTGCTCATAC ACTTCTGAAT ACAGTAGATG TTGAACTTAT CTATGGGGGA 
GTCAAATATG TACTTAAGGT GACTCGGCAG TCCCCCAACT CCTATGTGGT GATCATGAAT 
GGTTCATGCG TAGAAGTAGA TGTGCATCGG CTCAGTGATG GAGGGTTACT CCTGTCCTAT 
GATGGCAGCA GTTACACCAC ATACATGAAG GAAGAAGTGG ATAGATATCG TATCACAATT 
GGCAATAAGA CCTGTGTGTT TGAGAAGGAA AATGACCCTT CAGTGTTACG CTCACCTTCT 
GCTGGGAAGT TAATCCAGTA CATTGTGGAG GATGGAGGCC ATGTGTTTGC TGGCCAGTGC 
TATGCTGAAA TTGAGGTGAT GAAGATGGTA ATGACCTTAA CAGCTGCAGA GTCTGGCTGT 
ATCCATTATG TCAAACGGCC TGGAGCAGCT CTTGACCCTG GCTGTGTAAT AGCCAAAATG 
CAGTTGGACA ACCCCAGCAA GGTCCAACAG GCTGAGCTTC ACACAGGTAG TCTGCCACGG 
ATCCAGAGCA CTGCACTCAG AGGCGAGAAG CTCCATCGAG TGTTCCACTA TGTCCTGGAT 
AATCTGGTCA ACGTAATGAA TGGATACTGC CTTCCAGATC CTTTTTTTAG CAGCAGGGTA 
AAAGACTGGG TAGAGCGGTT GATGAAGACC CTCAGAGACC CCTCGTTACC TCTCCTAGAA 
TTACAAGATA TCATGACCAG TGTCTCTGGC CGTATTCCCC CCAATGTTGA GAAGTCTATC 
AAGAAGGAAA TGGCCCAGTA TGCCAGCAAT ATCACATCAG TCCTCTGTCA GTTTCCCAGC 
CAGCAGATTG CCAACATCCT AGATAGCCAT GCAGCTACGT TGAACCGGAA ATCTGAGCGG 
GAAGTCTTTT TCATGAACAC TCAGAGCATT GTCCAGCTGG TACAGAGATA CCGAAGTGGC 
ATCCGAGGCC ACATGAAGGC TGTGGTGATG GATCTGCTGC GGCAGTACCT GCGAGTAGAG 
ACACAGTTCC AGAATGGTCA CTATGACAAA TGTGTGTTTG CCCTTCGAGA AGAGAACAAG 
AGTGATATGA ACACTGTACT GAACTATATC TTCTCTCATG CTCAAGTCAC CAAGAAGAAT 
CTTCTGGTCA CAATGCTTAT TGATCAGTTG TGCGGTAGGG ACCCTACTCT CACTGATGAG 
CTGCTGAATA TTCTCACAGA GCTAACTCAA CTCAGTAAGA CCACCAATGC CAAAGTAGCA 
CTTCGAGCAC GCCAGGTTCT TATTGCCTCC CATTTGCCAT CATATGAGCT TCGCCATAAC 
CAAGTAGAGT CTATCTTCCT TTCAGCTATT GACATGTATG GACATCAGTT TTGCATTGAG 
AACCTGCAGA AACTCATCTT GTCTGAAACA TCTATTTTTG ATGTCCTACC AAACTTCTTC 
TATCACAGCA ACCAGGTAGT GAGGATGGCA GCTCTGGAGG TTTATGTTCG AAGGGCTTAT 
ATTGCCTATG AACTTAACAG CGTACAACAT CGCCAACTTA AGGACAACAC CTGTGTGGTG 
GAATTCCAAT TCATGCTACC CACATCTCAT CCAAATAGAG GGAACATCCC CACGCTAAAC 
AGAATGTCCT TCTCCTCCAA CCTCAACCAC TATGGCATGA CTCATGTAGC TAGTGTCAGC 
GATGTGCTTT TGGACAACTC ATTCACTCCA CCATGTCAAC GGATGGGCGG AATGGTCTCT 
TTTCGGACTT TCGAAGATTT TGTCAGGATC TTTGATGAAG TGATGGGCTG CTTCTGTGAT 
TCTCCACCGC AAAGCCCTAC ATTCCCTGAG GCAGGTCACA CATCTCTATA CGATGAAGAC 
AAGGTCCCCA GGGATGAACC AATTCACATT CTGAATGTGG CTATCAAGAC TGACTGTGAT 
ATTGAGGATG ACAGACTGGC AGCTATGTTC AGAGAGTTTA CCCAACAAAA TAAAGCTACC 
CTGGTTGAGC ATGGGATACG GCGCCTTACT TTCCTGGTTG CACAAAAGGA TTTCAGGAAA 
CAGGTCAACT ATGAGGTGGA TCAGAGATTT CATAGAGAAT TTCCTAAATT TTTCACCTTC 
CGAGCAAGGG ATAAGTTTGA GGAGGATCGT ATCTATCGTC ACCTGGAACC TGCCCTCGCT 
TTCCAGTTAG AGCTGAACCG GATGAGAAAT TTTGACCTTA CTGCCATTCC ATGTGCTAAT 
CATAAAATGC ATCTGTATCT TGGGGCAGCC AAGGTGGAGG TGGGCACAGA AGTGACAGAC 
TACAGGTTCT TTGTTCGTGC AATCATCAGA CATTCTGATC TGGTCACCAA GGAAGCTTCT 
TTTGAATACC TACAAAATGA AGGGGAGAGG CTGCTTCTAG AAGCCATGGA TGAGTTGGAA 
GTTGCTTTTA ACAATACAAA CGTTCGCACT GACTGTAACC ACATCTTCCT CAACTTTGTG 
CCCACGGTCA TCATGGACCC ATCAAAGATT GAGGAATCTG TGCGGAGCAT GGTAATGCGC 
TATGGAAGTC GGCTGTGGAA ATTACGTGTC CTCCAGGCAG AACTGAAAAT CAACATTCGC 
CTGACACCAA CTGGAAAAGC AATTCCCATC CGCCTCTTCC TGACAAACGA GTCTGGCTAT 
TACTTGGACA TCAGCTTGTA TAAGGAAGTG ACTGACTCCA GGACAGCACA GATCATGTTT 
CAGGCATATG GAGACAAACA GGGACCATTG CATGGAATGT TAATCAACAC TCCATACGTC 
ACCAAAGACC TGCTTCAATC AAAGAGATTC CAGGCACAAT CCTTGGGGAC AACATACATA 
TATGATATAC CAGAGATGTT TCGGCAGTCT CTGATCAAAC TCTGGGAGTC TATGTCCACT 
CAAGCATTTC TTCCATCACC CCCCCTGCCT TCCGACATGC TGACATATAC GGAGCTCGTG 
TTGGATGATC AAGGTCAGCT GGTCCACATG AACAGGCTTC CAGGAGGAAA TGAGATTGGC 
ATGGTAGCGT GGAAAATGAC CCTTAAAAGT CCTGAATACC CAGAAGGCCG AGATATCATT 
GTTATTGGCA ATGACATCAC ATACCGAATT GGGTCCTTTG GGCCCCAGGA GGATTTGCTG 
TTTCTCAGAG CTTCTGAGCT TGCCAGGGCA GAGGGTATCC CACGCATCTT TGTAGCAGCC 
AACAGTGGAG CAAGAATTGG ACTGGCAGAG GAAATTCGTC ATATGTTTCA TGTAGCCTGG 
GTAGATCCTG AGGATCCTTA CAAGGGATAC AAATATTTAT ATCTGACCCC TCAGGATTAT 
AAGAGAGTCA GTGCTCTCAA CTCTGTCCAT TGTGAACATG TAGAGGACGA AGGAGAATCC 
AGGTACAAGA TAACTGATAT TATTGGGAAG GAAGAGGGAC TTGGAGCAGA GAACCTTCGA 
GGCTCTGGAA TGATCGCTGG AGAATCCTCA TTGGCCTATG ATGAGATCAT CACCATCAGC 
CTGGTTACAT GCCGGGCCAT TGGGATTGGA GCTTACCTTG TCCGGCTGGG ACAGAGAACC 
ATCCAGGTTG AAAATTCTCA TTTAATTTTG ACTGGAGCTG GAGCCCTCAA CAAAGTCCTT 
GGGCGGGAAG TATACACCTC CAATAACCAG CTCGGGGGCA TCCAGATCAT GCACAACAAT 
GGGGTGACCC ACAGCATTGT TTGTGATGAC TTTGAAGGGG TTTTCACTGT CCTGCACTGG 
CTATCCTACA TGCCTAAGAA TGTGCGCAGT TTAGTTCCTC TCCTGAACTC CAAGGATCCT 
ATAGATAGAA TCATCGAGTT TGTTCCCACG AAGGCCCCAT ACGATCCTCG ATGGATGCTA 
GCAGGCCGCC CTCACCCAAC TCAGAAAGGT CAGTGGCTGA GTGGATTTTT TGACTATGGC 
TCTTTCTCAG AGATCATGCA ACCCTGGGCA CAAACTGTGG TGGTTGGCAG AGCCAGGCTA 
GGAGGAATAC CTGTGGGAGT AGTTGCTGTA GAAACCCGAA CAGTGGAACT AAGTATCCCA 
GCCGATCCTG CAAACCTGGA TTCTGAAGCC AAGATAATCC AGCAGGCTGG CCAGGTTTGG 
TTCCCAGACT CTGCGTTTAA GACCTATCAG GCTATCAAGG ACTTCAACCG TGAAGGACTG 
CCTCTGATGG TCTTTGCCAA CTGGAGAGGC TTCTCTGGTG GGATGAAAGA TATGTATGAC 
CAAGTGCTGA AGTTTGGTGC CTATATTGTG GATAGCTTAC GGGAGTGCTC CCAGCCTGTG 
CTGGTATACA TTCCTCCTCA GGCTGAGCTC CGGGGTGGCT CCTGGGTTGT GATTGACCCC 
ACCATCAACC CCCGGCATAT GGAGATGTAC GCTGACCGAG AAAGCAGGGG ATCAGTTCTG 
GAGCCAGAAG GGACAGTAGA AATCAAATTC CGCAGAAAGG ATCTGGTGAA AACCATGCGT 
CGGGTGGACC CAGTCTACAT TCGCTTGGCT GAGCGATTGG GCACCCCAGA GCTCAGCGCA 
GCTGAGCGGA AGGAGCTGGA GAACAAGTTG AAGGAGCGGG AGGAATTCCT AATTCCCATT 
TACCATCAGG TAGCCGTGCA GTTTGCTGAC TTGCACGACA CACCAGGCCG GATGCAGGAG 
AAGGGTGTTA TTAATGACAT CCTGGATTGG AAAACATCCC GTACCTTTTT CTATTGGCGG 
CTGAGGCGTC TCCTGCTGGA GGACCTGGTC AAAAAGAAAA TCCACAATGC CAACCCAGAG 
CTGACCGATG GCCAGATCCA GGCCATGCTA CGGCGCTGGT TTGTAGAAGT GGAAGGAACT 
GTGAAGGCCT ACGTTTGGGA CAATAACAAG GACCTGGTGG AGTGGCTGGA GAAACAGCTG 
ACCGAGGAAG ATGGTGTTCG CTCAGTAATC GAGGAAAACA TCAAATATAT CAGCAGAGAC 
TATGTCCTCA AGCAGATCCG CAGCTTGGTA CAGGCCAATC CCGAGGTTGC CATGGACTCC 
ATCGTCCATA TGACTCAGCA TATCTCACCC ACTCAGCGAG CAGAAGTCGT ACGGATCCTC 
TCCACGATGG ACTCCCCTTC AACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_548250.3
CDS	93..6959
Translation	MEGSAEEVKEMRYYMLQRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_548250.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_548250.6

ORF Insert Sequence:

ATGGAGGGTT CTGCAGAGGA AGTTAAGGAG ATGAGATATT ACATGCTTCA AAGGTCCAGC 
ATGTCTGGCT TGCATCTAGT GAAGCAAGGT CGAGACCGAA AGAAAATAGA CTCTCAGCGA 
GATTTCACTG TAGCTTCTCC AGCAGAATTT GTTACTCGTT TTGGGGGAAA TAAAGTGATT 
GAGAAGGTTC TTATTGCCAA CAATGGCATT GCAGCAGTGA AATGCATGCG GTCTATCCGT 
CGATGGTCTT ATGAGATGTT TCGAAATGAA CGTGCAATCC GATTTGTTGT TATGGTAACA 
CCTGAAGACC TGAAAGCCAA TGCAGAATAC ATTAAGATGG CGGATCACTA TGTGCCAGTG 
CCAGGAGGAC CAAATAACAA CAACTATGCG AACGTGGAAT TAATTCTTGA TATTGCTAAA 
AGGATTCCCG TACAAGCAGT GTGGGCTGGC TGGGGTCATG CTTCTGAGAA TCCCAAGCTC 
CCAGAACTTC TTTTGAAAAA TGGCATCGCC TTCATGGGCC CTCCAAGCCA GGCCATGTGG 
GCTTTGGGGG ATAAGATTGC ATCTTCTATA GTGGCTCAAA CTGCAGGTAT CCCAACTCTT 
CCCTGGAGTG GCAGTGGTCT TCGGGTGGAC TGGCATGAAA ATGATTTTTC CAAACGTATC 
TTAAATGTTC CCCAGGAATT ATACGAAAAA GGTTATGTGA AGGATGTGGA TGATGGGCTA 
AAGGCAGCGG AGGAAGTTGG ATATCCAGTA ATGATCAAGG CCTCAGAAGG AGGAGGAGGG 
AAAGGAATCA GAAAAGTCAA CAATGCAGAT GATTTCCCTA ACCTCTTCAG ACAGGTTCAA 
GCTGAAGTCC CTGGATCTCC TATATTTGTG ATGAGACTAG CCAAACAATC TCGTCATCTG 
GAGGTACAGA TCTTAGCAGA TCAATATGGC AATGCTATCT CTCTCTTTGG TCGTGACTGC 
TCTGTACAGC GCAGGCATCA GAAGATTATT GAGGAAGCAC CTGCTGCTAT TGCTACTCCA 
GCAGTATTTG AACATATGGA ACAGTGTGCT GTGAAACTTG CCAAAATGGT TGGTTATGTG 
AGTGCTGGGA CTGTGGAATA CCTCTACAGC CAGGATGGAA GCTTCTACTT TCTGGAACTG 
AACCCTCGGC TACAGGTGGA ACACCCTTGT ACAGAGATGG TGGCTGATGT CAACCTCCCT 
GCAGCACAAC TCCAGATTGC CATGGGGATC CCTCTGTACA GAATCAAGGA TATTCGTATG 
ATGTATGGGG TATCTCCCTG GGGTGATGCT CCCATTGATT TTGAAAATTC TGCTCACGTT 
CCTTGCCCAA GGGGCCATGT TATTGCCGCT CGGATCACTA GTGAAAATCC AGATGAGGGT 
TTTAAGCCCA GCTCAGGAAC AGTTCAGGAA CTGAATTTCC GTAGCAATAA GAATGTTTGG 
GGTTATTTCA GTGTTGCTGC TGCAGGGGGC CTTCATGAAT TTGCTGATTC TCAGTTTGGT 
CATTGCTTCT CCTGGGGAGA AAACAGAGAG GAAGCAATTT CAAACATGGT GGTGGCTTTG 
AAGGAGCTGT CTATTCGGGG TGACTTTCGA ACCACAGTTG AATACCTCAT CAAATTGTTG 
GAGACTGAAA GCTTTCAGTT GAATAGAATT GACACCGGCT GGCTGGACAG ACTGATAGCA 
GAGAAAGTAC AGGCAGAGCG ACCTGACACC ATGTTGGGAG TTGTTTGTGG GGCTCTCCAT 
GTGGCAGATG TGAGCCTACG GAATAGCATC TCTAACTTCC TTCATTCTTT AGAAAGGGGT 
CAAGTCCTTC CTGCTCATAC ACTTCTGAAT ACAGTAGATG TTGAACTTAT CTATGGGGGA 
GTCAAATATG TACTTAAGGT GACTCGGCAG TCCCCCAACT CCTATGTGGT GATCATGAAT 
GGTTCATGCG TAGAAGTAGA TGTGCATCGG CTCAGTGATG GAGGGTTACT CCTGTCCTAT 
GATGGCAGCA GTTACACCAC ATACATGAAG GAAGAAGTGG ATAGATATCG TATCACAATT 
GGCAATAAGA CCTGTGTGTT TGAGAAGGAA AATGACCCTT CAGTGTTACG CTCACCTTCT 
GCTGGGAAGT TAATCCAGTA CATTGTGGAG GATGGAGGCC ATGTGTTTGC TGGCCAGTGC 
TATGCTGAAA TTGAGGTGAT GAAGATGGTA ATGACCTTAA CAGCTGCAGA GTCTGGCTGT 
ATCCATTATG TCAAACGGCC TGGAGCAGCT CTTGACCCTG GCTGTGTAAT AGCCAAAATG 
CAGTTGGACA ACCCCAGCAA GGTCCAACAG GCTGAGCTTC ACACAGGTAG TCTGCCACGG 
ATCCAGAGCA CTGCACTCAG AGGCGAGAAG CTCCATCGAG TGTTCCACTA TGTCCTGGAT 
AATCTGGTCA ACGTAATGAA TGGATACTGC CTTCCAGATC CTTTTTTTAG CAGCAGGGTA 
AAAGACTGGG TAGAGCGGTT GATGAAGACC CTCAGAGACC CCTCGTTACC TCTCCTAGAA 
TTACAAGATA TCATGACCAG TGTCTCTGGC CGTATTCCCC CCAATGTTGA GAAGTCTATC 
AAGAAGGAAA TGGCCCAGTA TGCCAGCAAT ATCACATCAG TCCTCTGTCA GTTTCCCAGC 
CAGCAGATTG CCAACATCCT AGATAGCCAT GCAGCTACGT TGAACCGGAA ATCTGAGCGG 
GAAGTCTTTT TCATGAACAC TCAGAGCATT GTCCAGCTGG TACAGAGATA CCGAAGTGGC 
ATCCGAGGCC ACATGAAGGC TGTGGTGATG GATCTGCTGC GGCAGTACCT GCGAGTAGAG 
ACACAGTTCC AGAATGGTCA CTATGACAAA TGTGTGTTTG CCCTTCGAGA AGAGAACAAG 
AGTGATATGA ACACTGTACT GAACTATATC TTCTCTCATG CTCAAGTCAC CAAGAAGAAT 
CTTCTGGTCA CAATGCTTAT TGATCAGTTG TGCGGTAGGG ACCCTACTCT CACTGATGAG 
CTGCTGAATA TTCTCACAGA GCTAACTCAA CTCAGTAAGA CCACCAATGC CAAAGTAGCA 
CTTCGAGCAC GCCAGGTTCT TATTGCCTCC CATTTGCCAT CATATGAGCT TCGCCATAAC 
CAAGTAGAGT CTATCTTCCT TTCAGCTATT GACATGTATG GACATCAGTT TTGCATTGAG 
AACCTGCAGA AACTCATCTT GTCTGAAACA TCTATTTTTG ATGTCCTACC AAACTTCTTC 
TATCACAGCA ACCAGGTAGT GAGGATGGCA GCTCTGGAGG TTTATGTTCG AAGGGCTTAT 
ATTGCCTATG AACTTAACAG CGTACAACAT CGCCAACTTA AGGACAACAC CTGTGTGGTG 
GAATTCCAAT TCATGCTACC CACATCTCAT CCAAATAGAG GGAACATCCC CACGCTAAAC 
AGAATGTCCT TCTCCTCCAA CCTCAACCAC TATGGCATGA CTCATGTAGC TAGTGTCAGC 
GATGTGCTTT TGGACAACTC ATTCACTCCA CCATGTCAAC GGATGGGCGG AATGGTCTCT 
TTTCGGACTT TCGAAGATTT TGTCAGGATC TTTGATGAAG TGATGGGCTG CTTCTGTGAT 
TCTCCACCGC AAAGCCCTAC ATTCCCTGAG GCAGGTCACA CATCTCTATA CGATGAAGAC 
AAGGTCCCCA GGGATGAACC AATTCACATT CTGAATGTGG CTATCAAGAC TGACTGTGAT 
ATTGAGGATG ACAGACTGGC AGCTATGTTC AGAGAGTTTA CCCAACAAAA TAAAGCTACC 
CTGGTTGAGC ATGGGATACG GCGCCTTACT TTCCTGGTTG CACAAAAGGA TTTCAGGAAA 
CAGGTCAACT ATGAGGTGGA TCAGAGATTT CATAGAGAAT TTCCTAAATT TTTCACCTTC 
CGAGCAAGGG ATAAGTTTGA GGAGGATCGT ATCTATCGTC ACCTGGAACC TGCCCTCGCT 
TTCCAGTTAG AGCTGAACCG GATGAGAAAT TTTGACCTTA CTGCCATTCC ATGTGCTAAT 
CATAAAATGC ATCTGTATCT TGGGGCAGCC AAGGTGGAGG TGGGCACAGA AGTGACAGAC 
TACAGGTTCT TTGTTCGTGC AATCATCAGA CATTCTGATC TGGTCACCAA GGAAGCTTCT 
TTTGAATACC TACAAAATGA AGGGGAGAGG CTGCTTCTAG AAGCCATGGA TGAGTTGGAA 
GTTGCTTTTA ACAATACAAA CGTTCGCACT GACTGTAACC ACATCTTCCT CAACTTTGTG 
CCCACGGTCA TCATGGACCC ATCAAAGATT GAGGAATCTG TGCGGAGCAT GGTAATGCGC 
TATGGAAGTC GGCTGTGGAA ATTACGTGTC CTCCAGGCAG AACTGAAAAT CAACATTCGC 
CTGACACCAA CTGGAAAAGC AATTCCCATC CGCCTCTTCC TGACAAACGA GTCTGGCTAT 
TACTTGGACA TCAGCTTGTA TAAGGAAGTG ACTGACTCCA GGACAGCACA GATCATGTTT 
CAGGCATATG GAGACAAACA GGGACCATTG CATGGAATGT TAATCAACAC TCCATACGTC 
ACCAAAGACC TGCTTCAATC AAAGAGATTC CAGGCACAAT CCTTGGGGAC AACATACATA 
TATGATATAC CAGAGATGTT TCGGCAGTCT CTGATCAAAC TCTGGGAGTC TATGTCCACT 
CAAGCATTTC TTCCATCACC CCCCCTGCCT TCCGACATGC TGACATATAC GGAGCTCGTG 
TTGGATGATC AAGGTCAGCT GGTCCACATG AACAGGCTTC CAGGAGGAAA TGAGATTGGC 
ATGGTAGCGT GGAAAATGAC CCTTAAAAGT CCTGAATACC CAGAAGGCCG AGATATCATT 
GTTATTGGCA ATGACATCAC ATACCGAATT GGGTCCTTTG GGCCCCAGGA GGATTTGCTG 
TTTCTCAGAG CTTCTGAGCT TGCCAGGGCA GAGGGTATCC CACGCATCTT TGTAGCAGCC 
AACAGTGGAG CAAGAATTGG ACTGGCAGAG GAAATTCGTC ATATGTTTCA TGTAGCCTGG 
GTAGATCCTG AGGATCCTTA CAAGGGATAC AAATATTTAT ATCTGACCCC TCAGGATTAT 
AAGAGAGTCA GTGCTCTCAA CTCTGTCCAT TGTGAACATG TAGAGGACGA AGGAGAATCC 
AGGTACAAGA TAACTGATAT TATTGGGAAG GAAGAGGGAC TTGGAGCAGA GAACCTTCGA 
GGCTCTGGAA TGATCGCTGG AGAATCCTCA TTGGCCTATG ATGAGATCAT CACCATCAGC 
CTGGTTACAT GCCGGGCCAT TGGGATTGGA GCTTACCTTG TCCGGCTGGG ACAGAGAACC 
ATCCAGGTTG AAAATTCTCA TTTAATTTTG ACTGGAGCTG GAGCCCTCAA CAAAGTCCTT 
GGGCGGGAAG TATACACCTC CAATAACCAG CTCGGGGGCA TCCAGATCAT GCACAACAAT 
GGGGTGACCC ACAGCATTGT TTGTGATGAC TTTGAAGGGG TTTTCACTGT CCTGCACTGG 
CTATCCTACA TGCCTAAGAA TGTGCGCAGT TTAGTTCCTC TCCTGAACTC CAAGGATCCT 
ATAGATAGAA TCATCGAGTT TGTTCCCACG AAGGCCCCAT ACGATCCTCG ATGGATGCTA 
GCAGGCCGCC CTCACCCAAC TCAGAAAGGT CAGTGGCTGA GTGGATTTTT TGACTATGGC 
TCTTTCTCAG AGATCATGCA ACCCTGGGCA CAAACTGTGG TGGTTGGCAG AGCCAGGCTA 
GGAGGAATAC CTGTGGGAGT AGTTGCTGTA GAAACCCGAA CAGTGGAACT AAGTATCCCA 
GCCGATCCTG CAAACCTGGA TTCTGAAGCC AAGATAATCC AGCAGGCTGG CCAGGTTTGG 
TTCCCAGACT CTGCGTTTAA GACCTATCAG GCTATCAAGG ACTTCAACCG TGAAGGACTG 
CCTCTGATGG TCTTTGCCAA CTGGAGAGGC TTCTCTGGTG GGATGAAAGA TATGTATGAC 
CAAGTGCTGA AGTTTGGTGC CTATATTGTG GATAGCTTAC GGGAGTGCTC CCAGCCTGTG 
CTGGTATACA TTCCTCCTCA GGCTGAGCTC CGGGGTGGCT CCTGGGTTGT GATTGACCCC 
ACCATCAACC CCCGGCATAT GGAGATGTAC GCTGACCGAG AAAGCAGGGG ATCAGTTCTG 
GAGCCAGAAG GGACAGTAGA AATCAAATTC CGCAGAAAGG ATCTGGTGAA AACCATGCGT 
CGGGTGGACC CAGTCTACAT TCGCTTGGCT GAGCGATTGG GCACCCCAGA GCTCAGCGCA 
GCTGAGCGGA AGGAGCTGGA GAACAAGTTG AAGGAGCGGG AGGAATTCCT AATTCCCATT 
TACCATCAGG TAGCCGTGCA GTTTGCTGAC TTGCACGACA CACCAGGCCG GATGCAGGAG 
AAGGGTGTTA TTAATGACAT CCTGGATTGG AAAACATCCC GTACCTTTTT CTATTGGCGG 
CTGAGGCGTC TCCTGCTGGA GGACCTGGTC AAAAAGAAAA TCCACAATGC CAACCCAGAG 
CTGACCGATG GCCAGATCCA GGCCATGCTA CGGCGCTGGT TTGTAGAAGT GGAAGGAACT 
GTGAAGGCCT ACGTTTGGGA CAATAACAAG GACCTGGTGG AGTGGCTGGA GAAACAGCTG 
ACCGAGGAAG ATGGTGTTCG CTCAGTAATC GAGGAAAACA TCAAATATAT CAGCAGAGAC 
TATGTCCTCA AGCAGATCCG CAGCTTGGTA CAGGCCAATC CCGAGGTTGC CATGGACTCC 
ATCGTCCATA TGACTCAGCA TATCTCACCC ACTCAGCGAG CAGAAGTCGT ACGGATCCTC 
TCCACGATGG ACTCCCCTTC AACGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACACA ( XM_005624782.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624839.1 Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X9, mRNA.

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CloneID	OCe12095
Clone ID Related Accession (Same CDS sequence)	XM_005624782.2
Accession Version	XM_005624782.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	acetyl-CoA carboxylase 1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624782.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACAAT GCTGGGATCA CTGTGGGCTT CTAGTTGGAT TTTGTGCCAG GTCCAGCATG 
TCTGGCTTGC ATCTAGTGAA GCAAGGTCGA GACCGAAAGA AAATAGACTC TCAGCGAGAT 
TTCACTGTAG CTTCTCCAGC AGAATTTGTT ACTCGTTTTG GGGGAAATAA AGTGATTGAG 
AAGGTTCTTA TTGCCAACAA TGGCATTGCA GCAGTGAAAT GCATGCGGTC TATCCGTCGA 
TGGTCTTATG AGATGTTTCG AAATGAACGT GCAATCCGAT TTGTTGTTAT GGTAACACCT 
GAAGACCTGA AAGCCAATGC AGAATACATT AAGATGGCGG ATCACTATGT GCCAGTGCCA 
GGAGGACCAA ATAACAACAA CTATGCGAAC GTGGAATTAA TTCTTGATAT TGCTAAAAGG 
ATTCCCGTAC AAGCAGTGTG GGCTGGCTGG GGTCATGCTT CTGAGAATCC CAAGCTCCCA 
GAACTTCTTT TGAAAAATGG CATCGCCTTC ATGGGCCCTC CAAGCCAGGC CATGTGGGCT 
TTGGGGGATA AGATTGCATC TTCTATAGTG GCTCAAACTG CAGGTATCCC AACTCTTCCC 
TGGAGTGGCA GTGGTCTTCG GGTGGACTGG CATGAAAATG ATTTTTCCAA ACGTATCTTA 
AATGTTCCCC AGGAATTATA CGAAAAAGGT TATGTGAAGG ATGTGGATGA TGGGCTAAAG 
GCAGCGGAGG AAGTTGGATA TCCAGTAATG ATCAAGGCCT CAGAAGGAGG AGGAGGGAAA 
GGAATCAGAA AAGTCAACAA TGCAGATGAT TTCCCTAACC TCTTCAGACA GGTTCAAGCT 
GAAGTCCCTG GATCTCCTAT ATTTGTGATG AGACTAGCCA AACAATCTCG TCATCTGGAG 
GTACAGATCT TAGCAGATCA ATATGGCAAT GCTATCTCTC TCTTTGGTCG TGACTGCTCT 
GTACAGCGCA GGCATCAGAA GATTATTGAG GAAGCACCTG CTGCTATTGC TACTCCAGCA 
GTATTTGAAC ATATGGAACA GTGTGCTGTG AAACTTGCCA AAATGGTTGG TTATGTGAGT 
GCTGGGACTG TGGAATACCT CTACAGCCAG GATGGAAGCT TCTACTTTCT GGAACTGAAC 
CCTCGGCTAC AGGTGGAACA CCCTTGTACA GAGATGGTGG CTGATGTCAA CCTCCCTGCA 
GCACAACTCC AGATTGCCAT GGGGATCCCT CTGTACAGAA TCAAGGATAT TCGTATGATG 
TATGGGGTAT CTCCCTGGGG TGATGCTCCC ATTGATTTTG AAAATTCTGC TCACGTTCCT 
TGCCCAAGGG GCCATGTTAT TGCCGCTCGG ATCACTAGTG AAAATCCAGA TGAGGGTTTT 
AAGCCCAGCT CAGGAACAGT TCAGGAACTG AATTTCCGTA GCAATAAGAA TGTTTGGGGT 
TATTTCAGTG TTGCTGCTGC AGGGGGCCTT CATGAATTTG CTGATTCTCA GTTTGGTCAT 
TGCTTCTCCT GGGGAGAAAA CAGAGAGGAA GCAATTTCAA ACATGGTGGT GGCTTTGAAG 
GAGCTGTCTA TTCGGGGTGA CTTTCGAACC ACAGTTGAAT ACCTCATCAA ATTGTTGGAG 
ACTGAAAGCT TTCAGTTGAA TAGAATTGAC ACCGGCTGGC TGGACAGACT GATAGCAGAG 
AAAGTACAGG CAGAGCGACC TGACACCATG TTGGGAGTTG TTTGTGGGGC TCTCCATGTG 
GCAGATGTGA GCCTACGGAA TAGCATCTCT AACTTCCTTC ATTCTTTAGA AAGGGGTCAA 
GTCCTTCCTG CTCATACACT TCTGAATACA GTAGATGTTG AACTTATCTA TGGGGGAGTC 
AAATATGTAC TTAAGGTGAC TCGGCAGTCC CCCAACTCCT ATGTGGTGAT CATGAATGGT 
TCATGCGTAG AAGTAGATGT GCATCGGCTC AGTGATGGAG GGTTACTCCT GTCCTATGAT 
GGCAGCAGTT ACACCACATA CATGAAGGAA GAAGTGGATA GATATCGTAT CACAATTGGC 
AATAAGACCT GTGTGTTTGA GAAGGAAAAT GACCCTTCAG TGTTACGCTC ACCTTCTGCT 
GGGAAGTTAA TCCAGTACAT TGTGGAGGAT GGAGGCCATG TGTTTGCTGG CCAGTGCTAT 
GCTGAAATTG AGGTGATGAA GATGGTAATG ACCTTAACAG CTGCAGAGTC TGGCTGTATC 
CATTATGTCA AACGGCCTGG AGCAGCTCTT GACCCTGGCT GTGTAATAGC CAAAATGCAG 
TTGGACAACC CCAGCAAGGT CCAACAGGCT GAGCTTCACA CAGGTAGTCT GCCACGGATC 
CAGAGCACTG CACTCAGAGG CGAGAAGCTC CATCGAGTGT TCCACTATGT CCTGGATAAT 
CTGGTCAACG TAATGAATGG ATACTGCCTT CCAGATCCTT TTTTTAGCAG CAGGGTAAAA 
GACTGGGTAG AGCGGTTGAT GAAGACCCTC AGAGACCCCT CGTTACCTCT CCTAGAATTA 
CAAGATATCA TGACCAGTGT CTCTGGCCGT ATTCCCCCCA ATGTTGAGAA GTCTATCAAG 
AAGGAAATGG CCCAGTATGC CAGCAATATC ACATCAGTCC TCTGTCAGTT TCCCAGCCAG 
CAGATTGCCA ACATCCTAGA TAGCCATGCA GCTACGTTGA ACCGGAAATC TGAGCGGGAA 
GTCTTTTTCA TGAACACTCA GAGCATTGTC CAGCTGGTAC AGAGATACCG AAGTGGCATC 
CGAGGCCACA TGAAGGCTGT GGTGATGGAT CTGCTGCGGC AGTACCTGCG AGTAGAGACA 
CAGTTCCAGA ATGGTCACTA TGACAAATGT GTGTTTGCCC TTCGAGAAGA GAACAAGAGT 
GATATGAACA CTGTACTGAA CTATATCTTC TCTCATGCTC AAGTCACCAA GAAGAATCTT 
CTGGTCACAA TGCTTATTGA TCAGTTGTGC GGTAGGGACC CTACTCTCAC TGATGAGCTG 
CTGAATATTC TCACAGAGCT AACTCAACTC AGTAAGACCA CCAATGCCAA AGTAGCACTT 
CGAGCACGCC AGGTTCTTAT TGCCTCCCAT TTGCCATCAT ATGAGCTTCG CCATAACCAA 
GTAGAGTCTA TCTTCCTTTC AGCTATTGAC ATGTATGGAC ATCAGTTTTG CATTGAGAAC 
CTGCAGAAAC TCATCTTGTC TGAAACATCT ATTTTTGATG TCCTACCAAA CTTCTTCTAT 
CACAGCAACC AGGTAGTGAG GATGGCAGCT CTGGAGGTTT ATGTTCGAAG GGCTTATATT 
GCCTATGAAC TTAACAGCGT ACAACATCGC CAACTTAAGG ACAACACCTG TGTGGTGGAA 
TTCCAATTCA TGCTACCCAC ATCTCATCCA AATAGAGGGA ACATCCCCAC GCTAAACAGA 
ATGTCCTTCT CCTCCAACCT CAACCACTAT GGCATGACTC ATGTAGCTAG TGTCAGCGAT 
GTGCTTTTGG ACAACTCATT CACTCCACCA TGTCAACGGA TGGGCGGAAT GGTCTCTTTT 
CGGACTTTCG AAGATTTTGT CAGGATCTTT GATGAAGTGA TGGGCTGCTT CTGTGATTCT 
CCACCGCAAA GCCCTACATT CCCTGAGGCA GGTCACACAT CTCTATACGA TGAAGACAAG 
GTCCCCAGGG ATGAACCAAT TCACATTCTG AATGTGGCTA TCAAGACTGA CTGTGATATT 
GAGGATGACA GACTGGCAGC TATGTTCAGA GAGTTTACCC AACAAAATAA AGCTACCCTG 
GTTGAGCATG GGATACGGCG CCTTACTTTC CTGGTTGCAC AAAAGGATTT CAGGAAACAG 
GTCAACTATG AGGTGGATCA GAGATTTCAT AGAGAATTTC CTAAATTTTT CACCTTCCGA 
GCAAGGGATA AGTTTGAGGA GGATCGTATC TATCGTCACC TGGAACCTGC CCTCGCTTTC 
CAGTTAGAGC TGAACCGGAT GAGAAATTTT GACCTTACTG CCATTCCATG TGCTAATCAT 
AAAATGCATC TGTATCTTGG GGCAGCCAAG GTGGAGGTGG GCACAGAAGT GACAGACTAC 
AGGTTCTTTG TTCGTGCAAT CATCAGACAT TCTGATCTGG TCACCAAGGA AGCTTCTTTT 
GAATACCTAC AAAATGAAGG GGAGAGGCTG CTTCTAGAAG CCATGGATGA GTTGGAAGTT 
GCTTTTAACA ATACAAACGT TCGCACTGAC TGTAACCACA TCTTCCTCAA CTTTGTGCCC 
ACGGTCATCA TGGACCCATC AAAGATTGAG GAATCTGTGC GGAGCATGGT AATGCGCTAT 
GGAAGTCGGC TGTGGAAATT ACGTGTCCTC CAGGCAGAAC TGAAAATCAA CATTCGCCTG 
ACACCAACTG GAAAAGCAAT TCCCATCCGC CTCTTCCTGA CAAACGAGTC TGGCTATTAC 
TTGGACATCA GCTTGTATAA GGAAGTGACT GACTCCAGGA CAGCACAGAT CATGTTTCAG 
GCATATGGAG ACAAACAGGG ACCATTGCAT GGAATGTTAA TCAACACTCC ATACGTCACC 
AAAGACCTGC TTCAATCAAA GAGATTCCAG GCACAATCCT TGGGGACAAC ATACATATAT 
GATATACCAG AGATGTTTCG GCAGTCTCTG ATCAAACTCT GGGAGTCTAT GTCCACTCAA 
GCATTTCTTC CATCACCCCC CCTGCCTTCC GACATGCTGA CATATACGGA GCTCGTGTTG 
GATGATCAAG GTCAGCTGGT CCACATGAAC AGGCTTCCAG GAGGAAATGA GATTGGCATG 
GTAGCGTGGA AAATGACCCT TAAAAGTCCT GAATACCCAG AAGGCCGAGA TATCATTGTT 
ATTGGCAATG ACATCACATA CCGAATTGGG TCCTTTGGGC CCCAGGAGGA TTTGCTGTTT 
CTCAGAGCTT CTGAGCTTGC CAGGGCAGAG GGTATCCCAC GCATCTTTGT AGCAGCCAAC 
AGTGGAGCAA GAATTGGACT GGCAGAGGAA ATTCGTCATA TGTTTCATGT AGCCTGGGTA 
GATCCTGAGG ATCCTTACAA GGGATACAAA TATTTATATC TGACCCCTCA GGATTATAAG 
AGAGTCAGTG CTCTCAACTC TGTCCATTGT GAACATGTAG AGGACGAAGG AGAATCCAGG 
TACAAGATAA CTGATATTAT TGGGAAGGAA GAGGGACTTG GAGCAGAGAA CCTTCGAGGC 
TCTGGAATGA TCGCTGGAGA ATCCTCATTG GCCTATGATG AGATCATCAC CATCAGCCTG 
GTTACATGCC GGGCCATTGG GATTGGAGCT TACCTTGTCC GGCTGGGACA GAGAACCATC 
CAGGTTGAAA ATTCTCATTT AATTTTGACT GGAGCTGGAG CCCTCAACAA AGTCCTTGGG 
CGGGAAGTAT ACACCTCCAA TAACCAGCTC GGGGGCATCC AGATCATGCA CAACAATGGG 
GTGACCCACA GCATTGTTTG TGATGACTTT GAAGGGGTTT TCACTGTCCT GCACTGGCTA 
TCCTACATGC CTAAGAATGT GCGCAGTTTA GTTCCTCTCC TGAACTCCAA GGATCCTATA 
GATAGAATCA TCGAGTTTGT TCCCACGAAG GCCCCATACG ATCCTCGATG GATGCTAGCA 
GGCCGCCCTC ACCCAACTCA GAAAGGTCAG TGGCTGAGTG GATTTTTTGA CTATGGCTCT 
TTCTCAGAGA TCATGCAACC CTGGGCACAA ACTGTGGTGG TTGGCAGAGC CAGGCTAGGA 
GGAATACCTG TGGGAGTAGT TGCTGTAGAA ACCCGAACAG TGGAACTAAG TATCCCAGCC 
GATCCTGCAA ACCTGGATTC TGAAGCCAAG ATAATCCAGC AGGCTGGCCA GGTTTGGTTC 
CCAGACTCTG CGTTTAAGAC CTATCAGGCT ATCAAGGACT TCAACCGTGA AGGACTGCCT 
CTGATGGTCT TTGCCAACTG GAGAGGCTTC TCTGGTGGGA TGAAAGATAT GTATGACCAA 
GTGCTGAAGT TTGGTGCCTA TATTGTGGAT AGCTTACGGG AGTGCTCCCA GCCTGTGCTG 
GTATACATTC CTCCTCAGGC TGAGCTCCGG GGTGGCTCCT GGGTTGTGAT TGACCCCACC 
ATCAACCCCC GGCATATGGA GATGTACGCT GACCGAGAAA GCAGGGGATC AGTTCTGGAG 
CCAGAAGGGA CAGTAGAAAT CAAATTCCGC AGAAAGGATC TGGTGAAAAC CATGCGTCGG 
GTGGACCCAG TCTACATTCG CTTGGCTGAG CGATTGGGCA CCCCAGAGCT CAGCGCAGCT 
GAGCGGAAGG AGCTGGAGAA CAAGTTGAAG GAGCGGGAGG AATTCCTAAT TCCCATTTAC 
CATCAGGTAG CCGTGCAGTT TGCTGACTTG CACGACACAC CAGGCCGGAT GCAGGAGAAG 
GGTGTTATTA ATGACATCCT GGATTGGAAA ACATCCCGTA CCTTTTTCTA TTGGCGGCTG 
AGGCGTCTCC TGCTGGAGGA CCTGGTCAAA AAGAAAATCC ACAATGCCAA CCCAGAGCTG 
ACCGATGGCC AGATCCAGGC CATGCTACGG CGCTGGTTTG TAGAAGTGGA AGGAACTGTG 
AAGGCCTACG TTTGGGACAA TAACAAGGAC CTGGTGGAGT GGCTGGAGAA ACAGCTGACC 
GAGGAAGATG GTGTTCGCTC AGTAATCGAG GAAAACATCA AATATATCAG CAGAGACTAT 
GTCCTCAAGC AGATCCGCAG CTTGGTACAG GCCAATCCCG AGGTTGCCAT GGACTCCATC 
GTCCATATGA CTCAGCATAT CTCACCCACT CAGCGAGCAG AAGTCGTACG GATCCTCTCC 
ACGATGGACT CCCCTTCAAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624839.1
CDS	76..6939
Translation	MLQCWDHCGLLVGFCARSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWHENDFSKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYGGVKYVLKVTRQSPNSYVVIMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVLRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIQSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGAENLRGSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHSIVCDDFEGVFTVLHWLSYMPKNVRSLVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDSLRECSQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIRLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGVINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDSPST

Target ORF information:

RefSeq Version	XM_005624782.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris acetyl-CoA carboxylase alpha (ACACA), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624782.2

ORF Insert Sequence:

ATGTTACAAT GCTGGGATCA CTGTGGGCTT CTAGTTGGAT TTTGTGCCAG GTCCAGCATG 
TCTGGCTTGC ATCTAGTGAA GCAAGGTCGA GACCGAAAGA AAATAGACTC TCAGCGAGAT 
TTCACTGTAG CTTCTCCAGC AGAATTTGTT ACTCGTTTTG GGGGAAATAA AGTGATTGAG 
AAGGTTCTTA TTGCCAACAA TGGCATTGCA GCAGTGAAAT GCATGCGGTC TATCCGTCGA 
TGGTCTTATG AGATGTTTCG AAATGAACGT GCAATCCGAT TTGTTGTTAT GGTAACACCT 
GAAGACCTGA AAGCCAATGC AGAATACATT AAGATGGCGG ATCACTATGT GCCAGTGCCA 
GGAGGACCAA ATAACAACAA CTATGCGAAC GTGGAATTAA TTCTTGATAT TGCTAAAAGG 
ATTCCCGTAC AAGCAGTGTG GGCTGGCTGG GGTCATGCTT CTGAGAATCC CAAGCTCCCA 
GAACTTCTTT TGAAAAATGG CATCGCCTTC ATGGGCCCTC CAAGCCAGGC CATGTGGGCT 
TTGGGGGATA AGATTGCATC TTCTATAGTG GCTCAAACTG CAGGTATCCC AACTCTTCCC 
TGGAGTGGCA GTGGTCTTCG GGTGGACTGG CATGAAAATG ATTTTTCCAA ACGTATCTTA 
AATGTTCCCC AGGAATTATA CGAAAAAGGT TATGTGAAGG ATGTGGATGA TGGGCTAAAG 
GCAGCGGAGG AAGTTGGATA TCCAGTAATG ATCAAGGCCT CAGAAGGAGG AGGAGGGAAA 
GGAATCAGAA AAGTCAACAA TGCAGATGAT TTCCCTAACC TCTTCAGACA GGTTCAAGCT 
GAAGTCCCTG GATCTCCTAT ATTTGTGATG AGACTAGCCA AACAATCTCG TCATCTGGAG 
GTACAGATCT TAGCAGATCA ATATGGCAAT GCTATCTCTC TCTTTGGTCG TGACTGCTCT 
GTACAGCGCA GGCATCAGAA GATTATTGAG GAAGCACCTG CTGCTATTGC TACTCCAGCA 
GTATTTGAAC ATATGGAACA GTGTGCTGTG AAACTTGCCA AAATGGTTGG TTATGTGAGT 
GCTGGGACTG TGGAATACCT CTACAGCCAG GATGGAAGCT TCTACTTTCT GGAACTGAAC 
CCTCGGCTAC AGGTGGAACA CCCTTGTACA GAGATGGTGG CTGATGTCAA CCTCCCTGCA 
GCACAACTCC AGATTGCCAT GGGGATCCCT CTGTACAGAA TCAAGGATAT TCGTATGATG 
TATGGGGTAT CTCCCTGGGG TGATGCTCCC ATTGATTTTG AAAATTCTGC TCACGTTCCT 
TGCCCAAGGG GCCATGTTAT TGCCGCTCGG ATCACTAGTG AAAATCCAGA TGAGGGTTTT 
AAGCCCAGCT CAGGAACAGT TCAGGAACTG AATTTCCGTA GCAATAAGAA TGTTTGGGGT 
TATTTCAGTG TTGCTGCTGC AGGGGGCCTT CATGAATTTG CTGATTCTCA GTTTGGTCAT 
TGCTTCTCCT GGGGAGAAAA CAGAGAGGAA GCAATTTCAA ACATGGTGGT GGCTTTGAAG 
GAGCTGTCTA TTCGGGGTGA CTTTCGAACC ACAGTTGAAT ACCTCATCAA ATTGTTGGAG 
ACTGAAAGCT TTCAGTTGAA TAGAATTGAC ACCGGCTGGC TGGACAGACT GATAGCAGAG 
AAAGTACAGG CAGAGCGACC TGACACCATG TTGGGAGTTG TTTGTGGGGC TCTCCATGTG 
GCAGATGTGA GCCTACGGAA TAGCATCTCT AACTTCCTTC ATTCTTTAGA AAGGGGTCAA 
GTCCTTCCTG CTCATACACT TCTGAATACA GTAGATGTTG AACTTATCTA TGGGGGAGTC 
AAATATGTAC TTAAGGTGAC TCGGCAGTCC CCCAACTCCT ATGTGGTGAT CATGAATGGT 
TCATGCGTAG AAGTAGATGT GCATCGGCTC AGTGATGGAG GGTTACTCCT GTCCTATGAT 
GGCAGCAGTT ACACCACATA CATGAAGGAA GAAGTGGATA GATATCGTAT CACAATTGGC 
AATAAGACCT GTGTGTTTGA GAAGGAAAAT GACCCTTCAG TGTTACGCTC ACCTTCTGCT 
GGGAAGTTAA TCCAGTACAT TGTGGAGGAT GGAGGCCATG TGTTTGCTGG CCAGTGCTAT 
GCTGAAATTG AGGTGATGAA GATGGTAATG ACCTTAACAG CTGCAGAGTC TGGCTGTATC 
CATTATGTCA AACGGCCTGG AGCAGCTCTT GACCCTGGCT GTGTAATAGC CAAAATGCAG 
TTGGACAACC CCAGCAAGGT CCAACAGGCT GAGCTTCACA CAGGTAGTCT GCCACGGATC 
CAGAGCACTG CACTCAGAGG CGAGAAGCTC CATCGAGTGT TCCACTATGT CCTGGATAAT 
CTGGTCAACG TAATGAATGG ATACTGCCTT CCAGATCCTT TTTTTAGCAG CAGGGTAAAA 
GACTGGGTAG AGCGGTTGAT GAAGACCCTC AGAGACCCCT CGTTACCTCT CCTAGAATTA 
CAAGATATCA TGACCAGTGT CTCTGGCCGT ATTCCCCCCA ATGTTGAGAA GTCTATCAAG 
AAGGAAATGG CCCAGTATGC CAGCAATATC ACATCAGTCC TCTGTCAGTT TCCCAGCCAG 
CAGATTGCCA ACATCCTAGA TAGCCATGCA GCTACGTTGA ACCGGAAATC TGAGCGGGAA 
GTCTTTTTCA TGAACACTCA GAGCATTGTC CAGCTGGTAC AGAGATACCG AAGTGGCATC 
CGAGGCCACA TGAAGGCTGT GGTGATGGAT CTGCTGCGGC AGTACCTGCG AGTAGAGACA 
CAGTTCCAGA ATGGTCACTA TGACAAATGT GTGTTTGCCC TTCGAGAAGA GAACAAGAGT 
GATATGAACA CTGTACTGAA CTATATCTTC TCTCATGCTC AAGTCACCAA GAAGAATCTT 
CTGGTCACAA TGCTTATTGA TCAGTTGTGC GGTAGGGACC CTACTCTCAC TGATGAGCTG 
CTGAATATTC TCACAGAGCT AACTCAACTC AGTAAGACCA CCAATGCCAA AGTAGCACTT 
CGAGCACGCC AGGTTCTTAT TGCCTCCCAT TTGCCATCAT ATGAGCTTCG CCATAACCAA 
GTAGAGTCTA TCTTCCTTTC AGCTATTGAC ATGTATGGAC ATCAGTTTTG CATTGAGAAC 
CTGCAGAAAC TCATCTTGTC TGAAACATCT ATTTTTGATG TCCTACCAAA CTTCTTCTAT 
CACAGCAACC AGGTAGTGAG GATGGCAGCT CTGGAGGTTT ATGTTCGAAG GGCTTATATT 
GCCTATGAAC TTAACAGCGT ACAACATCGC CAACTTAAGG ACAACACCTG TGTGGTGGAA 
TTCCAATTCA TGCTACCCAC ATCTCATCCA AATAGAGGGA ACATCCCCAC GCTAAACAGA 
ATGTCCTTCT CCTCCAACCT CAACCACTAT GGCATGACTC ATGTAGCTAG TGTCAGCGAT 
GTGCTTTTGG ACAACTCATT CACTCCACCA TGTCAACGGA TGGGCGGAAT GGTCTCTTTT 
CGGACTTTCG AAGATTTTGT CAGGATCTTT GATGAAGTGA TGGGCTGCTT CTGTGATTCT 
CCACCGCAAA GCCCTACATT CCCTGAGGCA GGTCACACAT CTCTATACGA TGAAGACAAG 
GTCCCCAGGG ATGAACCAAT TCACATTCTG AATGTGGCTA TCAAGACTGA CTGTGATATT 
GAGGATGACA GACTGGCAGC TATGTTCAGA GAGTTTACCC AACAAAATAA AGCTACCCTG 
GTTGAGCATG GGATACGGCG CCTTACTTTC CTGGTTGCAC AAAAGGATTT CAGGAAACAG 
GTCAACTATG AGGTGGATCA GAGATTTCAT AGAGAATTTC CTAAATTTTT CACCTTCCGA 
GCAAGGGATA AGTTTGAGGA GGATCGTATC TATCGTCACC TGGAACCTGC CCTCGCTTTC 
CAGTTAGAGC TGAACCGGAT GAGAAATTTT GACCTTACTG CCATTCCATG TGCTAATCAT 
AAAATGCATC TGTATCTTGG GGCAGCCAAG GTGGAGGTGG GCACAGAAGT GACAGACTAC 
AGGTTCTTTG TTCGTGCAAT CATCAGACAT TCTGATCTGG TCACCAAGGA AGCTTCTTTT 
GAATACCTAC AAAATGAAGG GGAGAGGCTG CTTCTAGAAG CCATGGATGA GTTGGAAGTT 
GCTTTTAACA ATACAAACGT TCGCACTGAC TGTAACCACA TCTTCCTCAA CTTTGTGCCC 
ACGGTCATCA TGGACCCATC AAAGATTGAG GAATCTGTGC GGAGCATGGT AATGCGCTAT 
GGAAGTCGGC TGTGGAAATT ACGTGTCCTC CAGGCAGAAC TGAAAATCAA CATTCGCCTG 
ACACCAACTG GAAAAGCAAT TCCCATCCGC CTCTTCCTGA CAAACGAGTC TGGCTATTAC 
TTGGACATCA GCTTGTATAA GGAAGTGACT GACTCCAGGA CAGCACAGAT CATGTTTCAG 
GCATATGGAG ACAAACAGGG ACCATTGCAT GGAATGTTAA TCAACACTCC ATACGTCACC 
AAAGACCTGC TTCAATCAAA GAGATTCCAG GCACAATCCT TGGGGACAAC ATACATATAT 
GATATACCAG AGATGTTTCG GCAGTCTCTG ATCAAACTCT GGGAGTCTAT GTCCACTCAA 
GCATTTCTTC CATCACCCCC CCTGCCTTCC GACATGCTGA CATATACGGA GCTCGTGTTG 
GATGATCAAG GTCAGCTGGT CCACATGAAC AGGCTTCCAG GAGGAAATGA GATTGGCATG 
GTAGCGTGGA AAATGACCCT TAAAAGTCCT GAATACCCAG AAGGCCGAGA TATCATTGTT 
ATTGGCAATG ACATCACATA CCGAATTGGG TCCTTTGGGC CCCAGGAGGA TTTGCTGTTT 
CTCAGAGCTT CTGAGCTTGC CAGGGCAGAG GGTATCCCAC GCATCTTTGT AGCAGCCAAC 
AGTGGAGCAA GAATTGGACT GGCAGAGGAA ATTCGTCATA TGTTTCATGT AGCCTGGGTA 
GATCCTGAGG ATCCTTACAA GGGATACAAA TATTTATATC TGACCCCTCA GGATTATAAG 
AGAGTCAGTG CTCTCAACTC TGTCCATTGT GAACATGTAG AGGACGAAGG AGAATCCAGG 
TACAAGATAA CTGATATTAT TGGGAAGGAA GAGGGACTTG GAGCAGAGAA CCTTCGAGGC 
TCTGGAATGA TCGCTGGAGA ATCCTCATTG GCCTATGATG AGATCATCAC CATCAGCCTG 
GTTACATGCC GGGCCATTGG GATTGGAGCT TACCTTGTCC GGCTGGGACA GAGAACCATC 
CAGGTTGAAA ATTCTCATTT AATTTTGACT GGAGCTGGAG CCCTCAACAA AGTCCTTGGG 
CGGGAAGTAT ACACCTCCAA TAACCAGCTC GGGGGCATCC AGATCATGCA CAACAATGGG 
GTGACCCACA GCATTGTTTG TGATGACTTT GAAGGGGTTT TCACTGTCCT GCACTGGCTA 
TCCTACATGC CTAAGAATGT GCGCAGTTTA GTTCCTCTCC TGAACTCCAA GGATCCTATA 
GATAGAATCA TCGAGTTTGT TCCCACGAAG GCCCCATACG ATCCTCGATG GATGCTAGCA 
GGCCGCCCTC ACCCAACTCA GAAAGGTCAG TGGCTGAGTG GATTTTTTGA CTATGGCTCT 
TTCTCAGAGA TCATGCAACC CTGGGCACAA ACTGTGGTGG TTGGCAGAGC CAGGCTAGGA 
GGAATACCTG TGGGAGTAGT TGCTGTAGAA ACCCGAACAG TGGAACTAAG TATCCCAGCC 
GATCCTGCAA ACCTGGATTC TGAAGCCAAG ATAATCCAGC AGGCTGGCCA GGTTTGGTTC 
CCAGACTCTG CGTTTAAGAC CTATCAGGCT ATCAAGGACT TCAACCGTGA AGGACTGCCT 
CTGATGGTCT TTGCCAACTG GAGAGGCTTC TCTGGTGGGA TGAAAGATAT GTATGACCAA 
GTGCTGAAGT TTGGTGCCTA TATTGTGGAT AGCTTACGGG AGTGCTCCCA GCCTGTGCTG 
GTATACATTC CTCCTCAGGC TGAGCTCCGG GGTGGCTCCT GGGTTGTGAT TGACCCCACC 
ATCAACCCCC GGCATATGGA GATGTACGCT GACCGAGAAA GCAGGGGATC AGTTCTGGAG 
CCAGAAGGGA CAGTAGAAAT CAAATTCCGC AGAAAGGATC TGGTGAAAAC CATGCGTCGG 
GTGGACCCAG TCTACATTCG CTTGGCTGAG CGATTGGGCA CCCCAGAGCT CAGCGCAGCT 
GAGCGGAAGG AGCTGGAGAA CAAGTTGAAG GAGCGGGAGG AATTCCTAAT TCCCATTTAC 
CATCAGGTAG CCGTGCAGTT TGCTGACTTG CACGACACAC CAGGCCGGAT GCAGGAGAAG 
GGTGTTATTA ATGACATCCT GGATTGGAAA ACATCCCGTA CCTTTTTCTA TTGGCGGCTG 
AGGCGTCTCC TGCTGGAGGA CCTGGTCAAA AAGAAAATCC ACAATGCCAA CCCAGAGCTG 
ACCGATGGCC AGATCCAGGC CATGCTACGG CGCTGGTTTG TAGAAGTGGA AGGAACTGTG 
AAGGCCTACG TTTGGGACAA TAACAAGGAC CTGGTGGAGT GGCTGGAGAA ACAGCTGACC 
GAGGAAGATG GTGTTCGCTC AGTAATCGAG GAAAACATCA AATATATCAG CAGAGACTAT 
GTCCTCAAGC AGATCCGCAG CTTGGTACAG GCCAATCCCG AGGTTGCCAT GGACTCCATC 
GTCCATATGA CTCAGCATAT CTCACCCACT CAGCGAGCAG AAGTCGTACG GATCCTCTCC 
ACGATGGACT CCCCTTCAAC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ACACA
Entrez Gene ID	491130
Full Name	acetyl-CoA carboxylase alpha
General protein information	Preferred Names acetyl-CoA carboxylase alpha Names acetyl-CoA carboxylase 1 acetyl-Coenzyme A carboxylase alpha
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Canis lupus familiaris (dog)	ACACA	XP_005624832.1
Bos taurus (cattle)	ACACA	NP_776649.1
Mus musculus (house mouse)	Acaca	NP_579938.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005175	XP_314071.4
Schizosaccharomyces pombe (fission yeast)	cut6	NP_593271.1
Arabidopsis thaliana (thale cress)	ACC1	NP_174849.2
	ACACA	XP_001111823.1
Rattus norvegicus (Norway rat)	Acaca	NP_071529.1
Magnaporthe oryzae (rice blast fungus)	MGG_07613	XP_003711534.1
Drosophila melanogaster (fruit fly)	ACC	NP_724636.1
Saccharomyces cerevisiae (baker's yeast)	ACC1	NP_014413.1
Eremothecium gossypii	AGOS_AAR071W	NP_982612.1
Neurospora crassa	NCU08535	XP_963017.1
Pan troglodytes (chimpanzee)	ACACA	XP_511428.3
Gallus gallus (chicken)	ACACA	NP_990836.1
Arabidopsis thaliana (thale cress)	ACC2	NP_174850.4
Homo sapiens (human)	ACACA	NP_942131.1
Danio rerio (zebrafish)	acaca	NP_001258237.1
Kluyveromyces lactis	KLLA0F06072g	XP_455355.1
Xenopus tropicalis (tropical clawed frog)	acaca	XP_004911956.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ACACA cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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