cut6 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	cut6
Entrez Gene ID	2543344
Full Name	acetyl-CoA/biotin carboxylase
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018668.2	NP_593271.1	acetyl-CoA/biotin carboxylase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ACACA	XP_005624832.1
Bos taurus (cattle)	ACACA	NP_776649.1
Mus musculus (house mouse)	Acaca	NP_579938.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005175	XP_314071.4
Schizosaccharomyces pombe (fission yeast)	cut6	NP_593271.1
Arabidopsis thaliana (thale cress)	ACC1	NP_174849.2
	ACACA	XP_001111823.1
Rattus norvegicus (Norway rat)	Acaca	NP_071529.1
Magnaporthe oryzae (rice blast fungus)	MGG_07613	XP_003711534.1
Drosophila melanogaster (fruit fly)	ACC	NP_724636.1
Saccharomyces cerevisiae (baker's yeast)	ACC1	NP_014413.1
Eremothecium gossypii	AGOS_AAR071W	NP_982612.1
Neurospora crassa	NCU08535	XP_963017.1
Pan troglodytes (chimpanzee)	ACACA	XP_511428.3
Gallus gallus (chicken)	ACACA	NP_990836.1
Arabidopsis thaliana (thale cress)	ACC2	NP_174850.4
Homo sapiens (human)	ACACA	NP_942131.1
Danio rerio (zebrafish)	acaca	NP_001258237.1
Kluyveromyces lactis	KLLA0F06072g	XP_455355.1
Xenopus tropicalis (tropical clawed frog)	acaca	XP_004911956.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cut6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cut6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102686	NM_001018668.2 Latest version!	Schizosaccharomyces pombe acetyl-CoA/biotin carboxylase (cut6), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cut6 ( NM_001018668.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593271.1 Schizosaccharomyces pombe acetyl-CoA/biotin carboxylase (cut6), partial mRNA.

Price & Availability↑

CloneID	OSc102686
Clone ID Related Accession (Same CDS sequence)	NM_001018668.2
Accession Version	NM_001018668.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	acetyl-CoA/biotin carboxylase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018668.1. COMPLETENESS: incomplete on the 5' end.

ATGCGCCAGA GCGTTACCTC TTCCTCTTCC TGTAGCAAGC TCTACGCGAT AAAAGCAACC 
ATTTCCCTTC CACGACTCTT TTACCGTAGA CTGAGAACTA TGGCTCCTCG TGTAGCCTCC 
CATTTTCTGG GAGGCAATTC CTTAGATAAA GCACCTGCAG GAAAGGTGAA AGATTATATT 
GCATCACACG GAGGACACAC TGTTATCACG TCTATTCTTA TTGCTAATAA TGGTATTGCG 
GCCGTGAAAG AAATCCGAAG CATTCGGAAA TGGGCTTATG AAACCTTCAA TAATGAAAGA 
GCTATCAAGT TTACTGTTAT GGCAACGCCA GATGATTTAA AAGTTAATGC CGATTATATT 
CGTATGGCCG ATCAGTATGT CGAAGTACCT GGCGGCTCAA ATAACAATAA TTATGCTAAT 
GTCGAACTTA TCGTTGACAT TGCTGAACGT ATGAACGTCC ATGCTGTTTG GGCTGGTTGG 
GGACACGCAT CTGAAAACCC TAAATTGCCT GAGATGCTTT CTGCCAGTAG TAAGAAAATC 
GTTTTTATTG GTCCTCCAGG TAGCGCAATG CGTAGTCTTG GTGACAAAAT TAGTTCTACA 
ATCGTTGCTC AAAGTGCTCG TGTACCTTGT ATGTCTTGGT CCGGTAATGA ACTCGACCAA 
GTACGTATTG ATGAAGAGAC AAACATTGTT ACTGTTGACG ATGATGTTTA TCAAAAAGCC 
TGTATTCGCT CTGCAGAAGA AGGTATTGCC GTCGCCGAGA AGATTGGTTA TCCCGTCATG 
ATTAAGGCCT CTGAAGGCGG TGGTGGTAAA GGTATTCGTC AGGTTACTTC AACCGAAAAG 
TTTGCTCAAG CATTCCAACA AGTACTTGAT GAACTCCCTG GATCTCCCGT TTTTGTTATG 
AAACTTGCTG GACAAGCACG CCATTTGGAA GTTCAAATTT TAGCTGATCA ATATGGTAAT 
AATATTTCTC TTTTTGGTCG TGATTGTTCC GTTCAACGCC GTCATCAAAA AATTATTGAG 
GAGGCTCCTG TTACCATCGC ACCTGCCGCT ACCTTCCATG AAATGGAGCG TGCCGCCGTG 
CGTTTAGGTG AATTGGTCGG TTACGCTTCT GCTGGTACCA TTGAGTATCT TTATGAGCCA 
GAGAATGACA GGTTCTATTT CCTTGAACTG AACCCTCGTT TACAGGTCGA GCATCCAACT 
ACCGAAATGG TTTCTGGCGT TAATTTACCC GCTGCACAAC TTCAAGTTGC TATGGGTTTG 
CCTCTTAGTC GTATTCCACA CATTCGTGAG CTCTATGGCT TACCACGTGA TGGTGACTCT 
GAAATCGATT TTTTCTTTCA AAATCCCGAA TCTTTTAAAG TACAGAAGGT CCCTACTCCT 
AAAGGCCATT GTGTTGCCTG TCGTATTACG TCTGAAGATC CCGGCGAAGG ATTTAAACCA 
TCGAGCGGTA TGATTAAAGA TCTCAACTTT CGTTCTTCTA GCAATGTGTG GGGTTATTTC 
TCTGTTGGTA CTGCTGGTGG AATTCATGAG TTTGCGGATT CCCAATTCGG TCATATTTTT 
TCATTTGCAG AATCTCGTGA ATCCTCTCGC AAATCGATGG TGGTTGCGTT AAAAGAATTA 
TCTATTCGTG GTGATTTTAG AACTACTGTC GAATATCTCG TGCGTCTCCT TGAAACTAAG 
GAGTTTTCTG AAAATGAGTT TACCACAGGA TGGCTAGATC GGCTTATTGC ACAAAAAGTT 
ACATCTGCTC GTCCCGACAA GATGCTTGCT GTTGTATGTG GTGCTCTTGT CCGTGCTCAT 
GCTACTGCCG ATACTCAGTA CCGTGCTTTC AAATCCTACC TTGAACGCGG TCAAGTACCG 
TCCCGTGAAT TTTTGAAAAA TGTGTATGAT ATTGAATTTA TTTATGATAA CACTCGCTAT 
CGTTTTACCG CATCTCGTTC TTCTCCAGGC TCTTATCATT TGTTTTTAAA TGGTTCTCGT 
TGTACTGCTG GTGTCCGTTC TTTGACTGAT GGTGGATTGT TAGTTTTGCT AAACGGACAT 
TCCTATACAG TATACTATCG TGATGAGGTA ACTGGTACTC GTATATCTAT CGATAACCTT 
TCTTGTATGC TGGAACAAGA AAATGATCCT ACTCAATTAA GAACTCCTTC CCCTGGCAAG 
TTGGTTCGTT TCTTGGTTGA AACAGGTGAG CATATTAAAG CCGGTGAAGC GTATGCAGAG 
GTAGAAGTTA TGAAAATGAT TATGCCTTTA GTAGCAACCG AAGATGGTGT TGTTCAATTG 
ATAAAGCAAC CCGGTGCATC TTTAGACGCC GGTGATATTC TTGGAATACT CACGCTTGAT 
GATCCTAGCC GTGTCACCCA TGCATTACCA TTTGATGGTC AGCTTCCTAA TTGGGGTGAG 
CCTCAAATTG CGGGAAATAA GCCTTGTCAA CGCTATCATG CTCTTTTGTG TATTCTTTTG 
GACATTCTAA AGGGATATGA TAACCAAATC ATTCTCAACA GTACCTACAA TGAATTTGTT 
GAAGTCCTTC GTAATCATGA ATTGCCCTAT AGCGAATGGA GTGCTCATTA TTCAGCATTG 
GTTAATAGAA TCTCTCCTGT ACTTGATAAG CTTTTTGTAT CTATAATCGA AAAAGCCAGA 
TCTCGTAAAG CTGAATTTCC TGCCAAACAG CTTGAGGTTG CTATTCAGAC TTATTGTGAT 
GGTCAAAATT TGGCGACGAC TCAACAATTA AAGGTCCAAA TTGCACCTCT CCTTAAAATC 
ATATCTGACT ACAAAGACGG CCTCAAAGTT CATGAATACA ATGTTATTAA AGGTTTGCTC 
GAAGAATATT ATAATGTTGA AAAGTTGTTC TCTGGAATTA ATAAGCGAGA AGAAGATGTT 
ATTCTTCGTC TACGTGACGA AAATAAAGAT GATGTTGATA AAGTTATTGC GTTGGCTTTA 
TCTCATTCTC GTATAGGATC TAAGAATAAC TTGTTAATTA CAATTCTTGA TCTAATGAAG 
TCCGAACCAT CAACTTTTGT TTCTCTGTAC TTTAATGACA TTTTGAGGAA GCTTACAGAT 
TTGGATTCAA GGGTTACTTC TAAAGTGTCT CTAAAGGCTC GTGAGTTGTT AATTACATGT 
GCTATGCCTT CTCTTAATGA GCGATTCTCT CAAATGGAGC ACATATTGAA ATCGTCTGTA 
GTTGAAAGTC ATTATGGTGA TGCTAAATTC TCACACCGTA CACCATCTTT AGACATTCTG 
AAAGAATTGA TTGATTCTAA ATATACAGTC TTTGATGTTT TACCTGCTTT CTTTTGTCAC 
ACCGACCCAT GGGTATCTTT AGCTGCTCTT GAGGTATATG TTAGACGTGC TTATCGTGCC 
TACTCTGTTC TTGAAATCAA CTATCATACC GAGGCCGGAA CTCCGTATGT ACTCACGTGG 
CGTTTTCAGC TTCATTCAAG TGGTGCTCCG GGTTTGGGTG CCAACTCAAC TAATGGTTCC 
AATTTCCCTG CAAGCACTAC TCCTTCATAC GAAAACAGCA ATCGACGCCT GCAGTCTGTT 
AGTGATCTTT CTTGGTATGT CAATAAAACA GACTCTGAGC CATTCCGTTT TGGTACAATG 
ATTGCCGCAG AAACTTTCGA TGAATTGGAA AATAACCTTG CCCTTGCAAT CGACCGTTTA 
CCACTTTCTC GTAATTACTT TAATGCTGGT TTAACGTTGG ATGGCAATTC TTCTTCAGCT 
AACGATAACA CTCAAGAATT AACTAATGTA GTGAACGTTG CGTTAACCTC AACTGGTGAT 
TTGGATGATT CTGCTATTGT TAGCAAGCTT AACCAAATCC TTAGTGATTT CCGTGATGAT 
TTGCTTGAGC ATAATGTTAG AAGAGTGACA ATTGTTGGTG GCAGAATTAA CAAGTCTGCT 
TATCCTTCCT ACTATACTTA TCGTGTTTCC GCTGAACAAA AAGACGGCAA TCTTGTACAC 
TATAACGAAG ATGAGCGTAT TCGTCATATT GAACCTGCAT TGGCATTCCA ATTAGAATTG 
GGTCGTCTAT CGAACTTCAA TATTGAACCC GTTTTCACCG ATAATCATAA CATTCATGTT 
TATCGTGCTA CCGCCAAAAA TATGGATACA GATAAGCGAT TCTTTACTCG TGCATTAGTT 
AGACCAGGAA GATTACGTGA CGAGATACCT ACTGCTGAGT ATCTTATATC CGAAACCCAT 
CGTTTAATTA ATGATATTTT GGATGCTTTG GAAGTTATCG GTCATGAACA AACAGACTTG 
AATCATATTT TCATTAACTT TACACCAGCC TTTGGTCTTG CTCCTAAGCA AGTTGAAGCT 
GCCCTCGGCG GATTTTTGGA ACGTTTTGGC CGTCGTTTAT GGCGCTTGAG AGTAACTGCA 
GCTGAAATTC GTATTATTTG CACGGACCCA TCAACTAACA CTTTGTTTCC TCTTCGTGTC 
ATTATTTCTA ATGTTTCTGG ATTTGTTGTG AACGTTGAAA TTTATGCTGA AGTCAAGACT 
GAGAATAATT CTTGGATATT TAAGAGTATC GGACAACCTG GATCCATGCA TCTTCGCCCC 
ATCAGTACAC CTTATCCTAC CAAAGAATGG CTTCAACCTC GTCGTTACAA AGCTCAATTA 
ATGGGCACTA CTTTCGTTTA TGACTTCCCA GAATTATTCC GTCGCGCCTT CACCGATAGC 
TGGAAAAAGG TTCCAAATGG GCGATCCAAA GTTACTATAC CCCAGAATAT GTTTGAATGT 
AAGGAGCTTG TTGCTGACGA ACATGGTGTA TTACAGGAAG TCAATAGGGA GCCTGGAACT 
AACTCCTGTG GTATGGTAGC ATGGTGCATT ACTGTTAAGA CGCCTGAATA TCCTAATGGA 
CGAAAAATTA TCGTAGTGGC TAACGACATC ACTTTCCAAA TTGGTTCTTT TGGGCCCCAA 
GAGGATGAAT ACTTTTATAA AGTTACTCAA TTGGCACGTC AACGCGGTAT TCCTCGTATT 
TACCTCGCTG CCAATTCCGG TGCACGTATT GGAGTTGCTG ATGAAATCGT CCCTCTTTTC 
AATATTGCTT GGGTCGATCC CGATAGTCCA GAAAAGGGTT TTGATTATAT CTATCTTACT 
CCAGAGGCAT ATGAGCGTCT TCAGAAAGAA AATCCCAATA TTCTCACCAC TGAGGAGGTT 
GTTACTGAAA CTGGGGAACT TCGCCATAAG ATTACCACAA TCATTGGCTC AAGCGAGGGT 
CTTGGTGTTG AATGTTTGCG TGGATCCGGT CTGATTGCTG GTGTCACATC TCGCGCATAC 
AATGACATTT TTACATGTAC TTTGGTCACT TGTCGTGCTG TTGGTATTGG CGCGTACTTG 
GTTCGTCTCG GCCAAAGAGC TGTACAAATC GAAGGCCAAC CAATTATTCT AACAGGTGCA 
CCCGCCCTTA ACAAGGTTTT AGGCCGTGAG GTCTATACCT CCAACTTGCA ATTAGGTGGT 
ACTCAAGTTA TGCATAGAAA TGGTATATCC CATCTTACTA GTCAAGATGA TTTTGATGGC 
ATTTCGAAAA TTGTAAACTG GATTTCCTAT ATCCCCGATA AACGTAACAA TCCAGTACCA 
ATTTCACCAT CATCAGATAC ATGGGATCGT GATGTGGAGT TCTATCCTTC TCAAAATGGT 
TACGATCCTC GTTGGTTAAT TGCCGGAAAG GAAGATGAAG ATTCTTTCTT GTATGGTTTA 
TTTGACAAAG GATCTTTCCA GGAAACTTTG AATGGCTGGG CCAAGACTGT TGTTGTTGGT 
CGTGCTAGAA TGGGCGGAAT TCCTACTGGT GTGATTGCTG TCGAGACTCG TACTATTGAA 
AACACTGTAC CGGCTGATCC AGCTAACCCT GACTCTACTG AACAAGTATT AATGGAGGCT 
GGTCAAGTTT GGTATCCCAA CTCAGCCTTC AAGACTGCTC AAGCAATCAA TGACTTCAAC 
CATGGTGAAC AGTTACCTCT TTTTATTCTT GCTAATTGGC GTGGATTTTC TGGTGGTCAA 
CGTGATATGT TTAATGAGGT ACTCAAATAT GGTTCTTATA TCGTAGATGC TTTGGCTTCT 
TATAAACAAC CTGTATTTGT ATACATTCCT CCATTCAGTG AACTTAGAGG TGGCTCTTGG 
GTTGTAGTAG ATCCAACCAT CAATGAGGAT CAAATGGAAA TGTACGCAGA TGAAGAGAGT 
AGAGCTGGTG TTTTGGAACC TGAAGGTATG GTCAGTATTA AATTCAGACG TGAAAAGTTG 
CTTTCTTTGA TGCGACGCTG TGATCATAAA TATGCATCAT TGTGCAATGA GCTTAAAAGA 
GATGATTTGA GTGCTGATGA CCTTTCAACT ATAAAGGTCA AGTTGATGGA ACGCGAACAG 
AAGCTTATGC CAATTTATCA ACAAATTAGT ATTCATTTTG CCGACTTGCA TGATCGTGTT 
GGTCGTATGG TTGCAAAGAA GGTTGTCCGT AAACCGTTGA AATGGACAGA AGCTAGACGT 
TTCTTTTACT GGCGTCTCCG CAGACGTTTG AATGAACATT ATGCTCTTCA AAAGATTACC 
CAGCTCATTC CTTCCTTGAC TATCCGTGAA TCTCGTGAGT ATCTCCAGAA ATGGTATGAA 
GAGTGGTGTG GAAAGCAAGA TTGGGATGAA TCTGATAAGT CTGTTGTTTG TTGGATTGAG 
GAACATAACG ACGATTTGAG TAAGAGAACT CAGGAACTTA AGAGTACTTA TTACAGTGAG 
CGTCTTTCTA AACTCCTTCG TTCAGATAGG AAGGGAATGA TAGACAGCCT TGCACAAGTT 
TTGACCGAGC TCGACGAAAA TGAAAAGAAA GAATTGGCCG GAAAACTCGC GTCGGTTAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_593271.1
CDS	1..6843
Translation	MRQSVTSSSSCSKLYAIKATISLPRLFYRRLRTMAPRVASHFLGGNSLDKAPAGKVKDYIASHGGHTVITSILIANNGIAAVKEIRSIRKWAYETFNNERAIKFTVMATPDDLKVNADYIRMADQYVEVPGGSNNNNYANVELIVDIAERMNVHAVWAGWGHASENPKLPEMLSASSKKIVFIGPPGSAMRSLGDKISSTIVAQSARVPCMSWSGNELDQVRIDEETNIVTVDDDVYQKACIRSAEEGIAVAEKIGYPVMIKASEGGGGKGIRQVTSTEKFAQAFQQVLDELPGSPVFVMKLAGQARHLEVQILADQYGNNISLFGRDCSVQRRHQKIIEEAPVTIAPAATFHEMERAAVRLGELVGYASAGTIEYLYEPENDRFYFLELNPRLQVEHPTTEMVSGVNLPAAQLQVAMGLPLSRIPHIRELYGLPRDGDSEIDFFFQNPESFKVQKVPTPKGHCVACRITSEDPGEGFKPSSGMIKDLNFRSSSNVWGYFSVGTAGGIHEFADSQFGHIFSFAESRESSRKSMVVALKELSIRGDFRTTVEYLVRLLETKEFSENEFTTGWLDRLIAQKVTSARPDKMLAVVCGALVRAHATADTQYRAFKSYLERGQVPSREFLKNVYDIEFIYDNTRYRFTASRSSPGSYHLFLNGSRCTAGVRSLTDGGLLVLLNGHSYTVYYRDEVTGTRISIDNLSCMLEQENDPTQLRTPSPGKLVRFLVETGEHIKAGEAYAEVEVMKMIMPLVATEDGVVQLIKQPGASLDAGDILGILTLDDPSRVTHALPFDGQLPNWGEPQIAGNKPCQRYHALLCILLDILKGYDNQIILNSTYNEFVEVLRNHELPYSEWSAHYSALVNRISPVLDKLFVSIIEKARSRKAEFPAKQLEVAIQTYCDGQNLATTQQLKVQIAPLLKIISDYKDGLKVHEYNVIKGLLEEYYNVEKLFSGINKREEDVILRLRDENKDDVDKVIALALSHSRIGSKNNLLITILDLMKSEPSTFVSLYFNDILRKLTDLDSRVTSKVSLKARELLITCAMPSLNERFSQMEHILKSSVVESHYGDAKFSHRTPSLDILKELIDSKYTVFDVLPAFFCHTDPWVSLAALEVYVRRAYRAYSVLEINYHTEAGTPYVLTWRFQLHSSGAPGLGANSTNGSNFPASTTPSYENSNRRLQSVSDLSWYVNKTDSEPFRFGTMIAAETFDELENNLALAIDRLPLSRNYFNAGLTLDGNSSSANDNTQELTNVVNVALTSTGDLDDSAIVSKLNQILSDFRDDLLEHNVRRVTIVGGRINKSAYPSYYTYRVSAEQKDGNLVHYNEDERIRHIEPALAFQLELGRLSNFNIEPVFTDNHNIHVYRATAKNMDTDKRFFTRALVRPGRLRDEIPTAEYLISETHRLINDILDALEVIGHEQTDLNHIFINFTPAFGLAPKQVEAALGGFLERFGRRLWRLRVTAAEIRIICTDPSTNTLFPLRVIISNVSGFVVNVEIYAEVKTENNSWIFKSIGQPGSMHLRPISTPYPTKEWLQPRRYKAQLMGTTFVYDFPELFRRAFTDSWKKVPNGRSKVTIPQNMFECKELVADEHGVLQEVNREPGTNSCGMVAWCITVKTPEYPNGRKIIVVANDITFQIGSFGPQEDEYFYKVTQLARQRGIPRIYLAANSGARIGVADEIVPLFNIAWVDPDSPEKGFDYIYLTPEAYERLQKENPNILTTEEVVTETGELRHKITTIIGSSEGLGVECLRGSGLIAGVTSRAYNDIFTCTLVTCRAVGIGAYLVRLGQRAVQIEGQPIILTGAPALNKVLGREVYTSNLQLGGTQVMHRNGISHLTSQDDFDGISKIVNWISYIPDKRNNPVPISPSSDTWDRDVEFYPSQNGYDPRWLIAGKEDEDSFLYGLFDKGSFQETLNGWAKTVVVGRARMGGIPTGVIAVETRTIENTVPADPANPDSTEQVLMEAGQVWYPNSAFKTAQAINDFNHGEQLPLFILANWRGFSGGQRDMFNEVLKYGSYIVDALASYKQPVFVYIPPFSELRGGSWVVVDPTINEDQMEMYADEESRAGVLEPEGMVSIKFRREKLLSLMRRCDHKYASLCNELKRDDLSADDLSTIKVKLMEREQKLMPIYQQISIHFADLHDRVGRMVAKKVVRKPLKWTEARRFFYWRLRRRLNEHYALQKITQLIPSLTIRESREYLQKWYEEWCGKQDWDESDKSVVCWIEEHNDDLSKRTQELKSTYYSERLSKLLRSDRKGMIDSLAQVLTELDENEKKELAGKLASVN

Target ORF information:

RefSeq Version	NM_001018668.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe acetyl-CoA/biotin carboxylase (cut6), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018668.2

ORF Insert Sequence:

ATGCGCCAGA GCGTTACCTC TTCCTCTTCC TGTAGCAAGC TCTACGCGAT AAAAGCAACC 
ATTTCCCTTC CACGACTCTT TTACCGTAGA CTGAGAACTA TGGCTCCTCG TGTAGCCTCC 
CATTTTCTGG GAGGCAATTC CTTAGATAAA GCACCTGCAG GAAAGGTGAA AGATTATATT 
GCATCACACG GAGGACACAC TGTTATCACG TCTATTCTTA TTGCTAATAA TGGTATTGCG 
GCCGTGAAAG AAATCCGAAG CATTCGGAAA TGGGCTTATG AAACCTTCAA TAATGAAAGA 
GCTATCAAGT TTACTGTTAT GGCAACGCCA GATGATTTAA AAGTTAATGC CGATTATATT 
CGTATGGCCG ATCAGTATGT CGAAGTACCT GGCGGCTCAA ATAACAATAA TTATGCTAAT 
GTCGAACTTA TCGTTGACAT TGCTGAACGT ATGAACGTCC ATGCTGTTTG GGCTGGTTGG 
GGACACGCAT CTGAAAACCC TAAATTGCCT GAGATGCTTT CTGCCAGTAG TAAGAAAATC 
GTTTTTATTG GTCCTCCAGG TAGCGCAATG CGTAGTCTTG GTGACAAAAT TAGTTCTACA 
ATCGTTGCTC AAAGTGCTCG TGTACCTTGT ATGTCTTGGT CCGGTAATGA ACTCGACCAA 
GTACGTATTG ATGAAGAGAC AAACATTGTT ACTGTTGACG ATGATGTTTA TCAAAAAGCC 
TGTATTCGCT CTGCAGAAGA AGGTATTGCC GTCGCCGAGA AGATTGGTTA TCCCGTCATG 
ATTAAGGCCT CTGAAGGCGG TGGTGGTAAA GGTATTCGTC AGGTTACTTC AACCGAAAAG 
TTTGCTCAAG CATTCCAACA AGTACTTGAT GAACTCCCTG GATCTCCCGT TTTTGTTATG 
AAACTTGCTG GACAAGCACG CCATTTGGAA GTTCAAATTT TAGCTGATCA ATATGGTAAT 
AATATTTCTC TTTTTGGTCG TGATTGTTCC GTTCAACGCC GTCATCAAAA AATTATTGAG 
GAGGCTCCTG TTACCATCGC ACCTGCCGCT ACCTTCCATG AAATGGAGCG TGCCGCCGTG 
CGTTTAGGTG AATTGGTCGG TTACGCTTCT GCTGGTACCA TTGAGTATCT TTATGAGCCA 
GAGAATGACA GGTTCTATTT CCTTGAACTG AACCCTCGTT TACAGGTCGA GCATCCAACT 
ACCGAAATGG TTTCTGGCGT TAATTTACCC GCTGCACAAC TTCAAGTTGC TATGGGTTTG 
CCTCTTAGTC GTATTCCACA CATTCGTGAG CTCTATGGCT TACCACGTGA TGGTGACTCT 
GAAATCGATT TTTTCTTTCA AAATCCCGAA TCTTTTAAAG TACAGAAGGT CCCTACTCCT 
AAAGGCCATT GTGTTGCCTG TCGTATTACG TCTGAAGATC CCGGCGAAGG ATTTAAACCA 
TCGAGCGGTA TGATTAAAGA TCTCAACTTT CGTTCTTCTA GCAATGTGTG GGGTTATTTC 
TCTGTTGGTA CTGCTGGTGG AATTCATGAG TTTGCGGATT CCCAATTCGG TCATATTTTT 
TCATTTGCAG AATCTCGTGA ATCCTCTCGC AAATCGATGG TGGTTGCGTT AAAAGAATTA 
TCTATTCGTG GTGATTTTAG AACTACTGTC GAATATCTCG TGCGTCTCCT TGAAACTAAG 
GAGTTTTCTG AAAATGAGTT TACCACAGGA TGGCTAGATC GGCTTATTGC ACAAAAAGTT 
ACATCTGCTC GTCCCGACAA GATGCTTGCT GTTGTATGTG GTGCTCTTGT CCGTGCTCAT 
GCTACTGCCG ATACTCAGTA CCGTGCTTTC AAATCCTACC TTGAACGCGG TCAAGTACCG 
TCCCGTGAAT TTTTGAAAAA TGTGTATGAT ATTGAATTTA TTTATGATAA CACTCGCTAT 
CGTTTTACCG CATCTCGTTC TTCTCCAGGC TCTTATCATT TGTTTTTAAA TGGTTCTCGT 
TGTACTGCTG GTGTCCGTTC TTTGACTGAT GGTGGATTGT TAGTTTTGCT AAACGGACAT 
TCCTATACAG TATACTATCG TGATGAGGTA ACTGGTACTC GTATATCTAT CGATAACCTT 
TCTTGTATGC TGGAACAAGA AAATGATCCT ACTCAATTAA GAACTCCTTC CCCTGGCAAG 
TTGGTTCGTT TCTTGGTTGA AACAGGTGAG CATATTAAAG CCGGTGAAGC GTATGCAGAG 
GTAGAAGTTA TGAAAATGAT TATGCCTTTA GTAGCAACCG AAGATGGTGT TGTTCAATTG 
ATAAAGCAAC CCGGTGCATC TTTAGACGCC GGTGATATTC TTGGAATACT CACGCTTGAT 
GATCCTAGCC GTGTCACCCA TGCATTACCA TTTGATGGTC AGCTTCCTAA TTGGGGTGAG 
CCTCAAATTG CGGGAAATAA GCCTTGTCAA CGCTATCATG CTCTTTTGTG TATTCTTTTG 
GACATTCTAA AGGGATATGA TAACCAAATC ATTCTCAACA GTACCTACAA TGAATTTGTT 
GAAGTCCTTC GTAATCATGA ATTGCCCTAT AGCGAATGGA GTGCTCATTA TTCAGCATTG 
GTTAATAGAA TCTCTCCTGT ACTTGATAAG CTTTTTGTAT CTATAATCGA AAAAGCCAGA 
TCTCGTAAAG CTGAATTTCC TGCCAAACAG CTTGAGGTTG CTATTCAGAC TTATTGTGAT 
GGTCAAAATT TGGCGACGAC TCAACAATTA AAGGTCCAAA TTGCACCTCT CCTTAAAATC 
ATATCTGACT ACAAAGACGG CCTCAAAGTT CATGAATACA ATGTTATTAA AGGTTTGCTC 
GAAGAATATT ATAATGTTGA AAAGTTGTTC TCTGGAATTA ATAAGCGAGA AGAAGATGTT 
ATTCTTCGTC TACGTGACGA AAATAAAGAT GATGTTGATA AAGTTATTGC GTTGGCTTTA 
TCTCATTCTC GTATAGGATC TAAGAATAAC TTGTTAATTA CAATTCTTGA TCTAATGAAG 
TCCGAACCAT CAACTTTTGT TTCTCTGTAC TTTAATGACA TTTTGAGGAA GCTTACAGAT 
TTGGATTCAA GGGTTACTTC TAAAGTGTCT CTAAAGGCTC GTGAGTTGTT AATTACATGT 
GCTATGCCTT CTCTTAATGA GCGATTCTCT CAAATGGAGC ACATATTGAA ATCGTCTGTA 
GTTGAAAGTC ATTATGGTGA TGCTAAATTC TCACACCGTA CACCATCTTT AGACATTCTG 
AAAGAATTGA TTGATTCTAA ATATACAGTC TTTGATGTTT TACCTGCTTT CTTTTGTCAC 
ACCGACCCAT GGGTATCTTT AGCTGCTCTT GAGGTATATG TTAGACGTGC TTATCGTGCC 
TACTCTGTTC TTGAAATCAA CTATCATACC GAGGCCGGAA CTCCGTATGT ACTCACGTGG 
CGTTTTCAGC TTCATTCAAG TGGTGCTCCG GGTTTGGGTG CCAACTCAAC TAATGGTTCC 
AATTTCCCTG CAAGCACTAC TCCTTCATAC GAAAACAGCA ATCGACGCCT GCAGTCTGTT 
AGTGATCTTT CTTGGTATGT CAATAAAACA GACTCTGAGC CATTCCGTTT TGGTACAATG 
ATTGCCGCAG AAACTTTCGA TGAATTGGAA AATAACCTTG CCCTTGCAAT CGACCGTTTA 
CCACTTTCTC GTAATTACTT TAATGCTGGT TTAACGTTGG ATGGCAATTC TTCTTCAGCT 
AACGATAACA CTCAAGAATT AACTAATGTA GTGAACGTTG CGTTAACCTC AACTGGTGAT 
TTGGATGATT CTGCTATTGT TAGCAAGCTT AACCAAATCC TTAGTGATTT CCGTGATGAT 
TTGCTTGAGC ATAATGTTAG AAGAGTGACA ATTGTTGGTG GCAGAATTAA CAAGTCTGCT 
TATCCTTCCT ACTATACTTA TCGTGTTTCC GCTGAACAAA AAGACGGCAA TCTTGTACAC 
TATAACGAAG ATGAGCGTAT TCGTCATATT GAACCTGCAT TGGCATTCCA ATTAGAATTG 
GGTCGTCTAT CGAACTTCAA TATTGAACCC GTTTTCACCG ATAATCATAA CATTCATGTT 
TATCGTGCTA CCGCCAAAAA TATGGATACA GATAAGCGAT TCTTTACTCG TGCATTAGTT 
AGACCAGGAA GATTACGTGA CGAGATACCT ACTGCTGAGT ATCTTATATC CGAAACCCAT 
CGTTTAATTA ATGATATTTT GGATGCTTTG GAAGTTATCG GTCATGAACA AACAGACTTG 
AATCATATTT TCATTAACTT TACACCAGCC TTTGGTCTTG CTCCTAAGCA AGTTGAAGCT 
GCCCTCGGCG GATTTTTGGA ACGTTTTGGC CGTCGTTTAT GGCGCTTGAG AGTAACTGCA 
GCTGAAATTC GTATTATTTG CACGGACCCA TCAACTAACA CTTTGTTTCC TCTTCGTGTC 
ATTATTTCTA ATGTTTCTGG ATTTGTTGTG AACGTTGAAA TTTATGCTGA AGTCAAGACT 
GAGAATAATT CTTGGATATT TAAGAGTATC GGACAACCTG GATCCATGCA TCTTCGCCCC 
ATCAGTACAC CTTATCCTAC CAAAGAATGG CTTCAACCTC GTCGTTACAA AGCTCAATTA 
ATGGGCACTA CTTTCGTTTA TGACTTCCCA GAATTATTCC GTCGCGCCTT CACCGATAGC 
TGGAAAAAGG TTCCAAATGG GCGATCCAAA GTTACTATAC CCCAGAATAT GTTTGAATGT 
AAGGAGCTTG TTGCTGACGA ACATGGTGTA TTACAGGAAG TCAATAGGGA GCCTGGAACT 
AACTCCTGTG GTATGGTAGC ATGGTGCATT ACTGTTAAGA CGCCTGAATA TCCTAATGGA 
CGAAAAATTA TCGTAGTGGC TAACGACATC ACTTTCCAAA TTGGTTCTTT TGGGCCCCAA 
GAGGATGAAT ACTTTTATAA AGTTACTCAA TTGGCACGTC AACGCGGTAT TCCTCGTATT 
TACCTCGCTG CCAATTCCGG TGCACGTATT GGAGTTGCTG ATGAAATCGT CCCTCTTTTC 
AATATTGCTT GGGTCGATCC CGATAGTCCA GAAAAGGGTT TTGATTATAT CTATCTTACT 
CCAGAGGCAT ATGAGCGTCT TCAGAAAGAA AATCCCAATA TTCTCACCAC TGAGGAGGTT 
GTTACTGAAA CTGGGGAACT TCGCCATAAG ATTACCACAA TCATTGGCTC AAGCGAGGGT 
CTTGGTGTTG AATGTTTGCG TGGATCCGGT CTGATTGCTG GTGTCACATC TCGCGCATAC 
AATGACATTT TTACATGTAC TTTGGTCACT TGTCGTGCTG TTGGTATTGG CGCGTACTTG 
GTTCGTCTCG GCCAAAGAGC TGTACAAATC GAAGGCCAAC CAATTATTCT AACAGGTGCA 
CCCGCCCTTA ACAAGGTTTT AGGCCGTGAG GTCTATACCT CCAACTTGCA ATTAGGTGGT 
ACTCAAGTTA TGCATAGAAA TGGTATATCC CATCTTACTA GTCAAGATGA TTTTGATGGC 
ATTTCGAAAA TTGTAAACTG GATTTCCTAT ATCCCCGATA AACGTAACAA TCCAGTACCA 
ATTTCACCAT CATCAGATAC ATGGGATCGT GATGTGGAGT TCTATCCTTC TCAAAATGGT 
TACGATCCTC GTTGGTTAAT TGCCGGAAAG GAAGATGAAG ATTCTTTCTT GTATGGTTTA 
TTTGACAAAG GATCTTTCCA GGAAACTTTG AATGGCTGGG CCAAGACTGT TGTTGTTGGT 
CGTGCTAGAA TGGGCGGAAT TCCTACTGGT GTGATTGCTG TCGAGACTCG TACTATTGAA 
AACACTGTAC CGGCTGATCC AGCTAACCCT GACTCTACTG AACAAGTATT AATGGAGGCT 
GGTCAAGTTT GGTATCCCAA CTCAGCCTTC AAGACTGCTC AAGCAATCAA TGACTTCAAC 
CATGGTGAAC AGTTACCTCT TTTTATTCTT GCTAATTGGC GTGGATTTTC TGGTGGTCAA 
CGTGATATGT TTAATGAGGT ACTCAAATAT GGTTCTTATA TCGTAGATGC TTTGGCTTCT 
TATAAACAAC CTGTATTTGT ATACATTCCT CCATTCAGTG AACTTAGAGG TGGCTCTTGG 
GTTGTAGTAG ATCCAACCAT CAATGAGGAT CAAATGGAAA TGTACGCAGA TGAAGAGAGT 
AGAGCTGGTG TTTTGGAACC TGAAGGTATG GTCAGTATTA AATTCAGACG TGAAAAGTTG 
CTTTCTTTGA TGCGACGCTG TGATCATAAA TATGCATCAT TGTGCAATGA GCTTAAAAGA 
GATGATTTGA GTGCTGATGA CCTTTCAACT ATAAAGGTCA AGTTGATGGA ACGCGAACAG 
AAGCTTATGC CAATTTATCA ACAAATTAGT ATTCATTTTG CCGACTTGCA TGATCGTGTT 
GGTCGTATGG TTGCAAAGAA GGTTGTCCGT AAACCGTTGA AATGGACAGA AGCTAGACGT 
TTCTTTTACT GGCGTCTCCG CAGACGTTTG AATGAACATT ATGCTCTTCA AAAGATTACC 
CAGCTCATTC CTTCCTTGAC TATCCGTGAA TCTCGTGAGT ATCTCCAGAA ATGGTATGAA 
GAGTGGTGTG GAAAGCAAGA TTGGGATGAA TCTGATAAGT CTGTTGTTTG TTGGATTGAG 
GAACATAACG ACGATTTGAG TAAGAGAACT CAGGAACTTA AGAGTACTTA TTACAGTGAG 
CGTCTTTCTA AACTCCTTCG TTCAGATAGG AAGGGAATGA TAGACAGCCT TGCACAAGTT 
TTGACCGAGC TCGACGAAAA TGAAAAGAAA GAATTGGCCG GAAAACTCGC GTCGGTTAAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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cut6 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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