acaca gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021476200.1	XP_021331875.1	acetyl-CoA carboxylase 1 isoform X15
XM_017356113.1	XP_017211602.1	acetyl-CoA carboxylase 1 isoform X4
XM_017356115.1	XP_017211604.1	acetyl-CoA carboxylase 1 isoform X6
XM_021476193.1	XP_021331868.1	acetyl-CoA carboxylase 1 isoform X1
XM_021476197.1	XP_021331872.1	acetyl-CoA carboxylase 1 isoform X6
XM_017356112.2	XP_017211601.1	acetyl-CoA carboxylase 1 isoform X9
XM_017356113.2	XP_017211602.1	acetyl-CoA carboxylase 1 isoform X10
XM_017356117.2	XP_017211606.1	acetyl-CoA carboxylase 1 isoform X14
XM_017356109.1	XP_017211598.1	acetyl-CoA carboxylase 1 isoform X1
XM_017356111.1	XP_017211600.1	acetyl-CoA carboxylase 1 isoform X2
XM_017356114.1	XP_017211603.1	acetyl-CoA carboxylase 1 isoform X5
XM_021476192.1	XP_021331867.1	acetyl-CoA carboxylase 1 isoform X1
XM_017356115.2	XP_017211604.1	acetyl-CoA carboxylase 1 isoform X13
XM_017356110.1	XP_017211599.1	acetyl-CoA carboxylase 1 isoform X1
XM_017356117.1	XP_017211606.1	acetyl-CoA carboxylase 1 isoform X8
XM_021476196.1	XP_021331871.1	acetyl-CoA carboxylase 1 isoform X4
XM_017356109.2	XP_017211598.1	acetyl-CoA carboxylase 1 isoform X5
XM_021476199.1	XP_021331874.1	acetyl-CoA carboxylase 1 isoform X12
XM_017356112.1	XP_017211601.1	acetyl-CoA carboxylase 1 isoform X3
XM_017356116.1	XP_017211605.1	acetyl-CoA carboxylase 1 isoform X7
XM_021476194.1	XP_021331869.1	acetyl-CoA carboxylase 1 isoform X2
XM_021476195.1	XP_021331870.1	acetyl-CoA carboxylase 1 isoform X3
XM_017356111.2	XP_017211600.1	acetyl-CoA carboxylase 1 isoform X7
XM_021476198.1	XP_021331873.1	acetyl-CoA carboxylase 1 isoform X8
XM_017356114.2	XP_017211603.1	acetyl-CoA carboxylase 1 isoform X11
NM_001271308.1	NP_001258237.1	acetyl-CoA carboxylase 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa59097	XM_017356116.1 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa142651	XM_021476194.1 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa142652	XM_021476195.1 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa142653	XM_021476196.1 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa142654	XM_021476197.1 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa142655	XM_021476198.1 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa142656	XM_021476199.1 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa142657	XM_021476200.1 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa43079	XM_021476192.1 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa43079	XM_021476193.1 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa59092	XM_017356111.2 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa59093	XM_017356112.2 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa59094	XM_017356113.2 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa59095	XM_017356114.2 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa59096	XM_017356115.2 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa59098	XM_017356117.2 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa03022	NM_001271308.1 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa03022	XM_017356110.1 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa03022	XM_017356109.2 Latest version!	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
View Old Accession Versions >>
ODa59094	XM_017356113.1	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa59096	XM_017356115.1	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa59098	XM_017356117.1	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa03022	XM_017356109.1	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa59093	XM_017356112.1	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa59092	XM_017356111.1	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa59095	XM_017356114.1	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
Hide Old Accession Versions >>

acaca ( XM_017356116.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211605.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa59097
Clone ID Related Accession (Same CDS sequence)	XM_017356116.1
Accession Version	XM_017356116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGC ATTCAGCAAT CCCATCAATA CAGACAATTT TGAAAACAAC ACTGGCCGAA 
ATGCAGCCCC AAACCAGGAC AAACCAGAGA CTCCAACATG TTTCACTGAA GGCTGCAAGC 
ACTCATTTTT CATTCTCTCT CCAACTCTGT CCAACTTCTA TGTATTTAAC AATTAGACCA 
AAAAACTACA TCTCTAACCT ATATTTCTTT TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211605.1
CDS	242..5017
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRKLSAPLQDYKPPANTANTSGATEAASDSAESEDRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKHSAIPSIQTILKTTLAEMQPQTRTNQRLQHVSLKAASTHFSFSLQLCPTSMYLTIRPKNYISNLYFFL

Target ORF information:

RefSeq Version	XM_017356116.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356116.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGC ATTCAGCAAT CCCATCAATA CAGACAATTT TGAAAACAAC ACTGGCCGAA 
ATGCAGCCCC AAACCAGGAC AAACCAGAGA CTCCAACATG TTTCACTGAA GGCTGCAAGC 
ACTCATTTTT CATTCTCTCT CCAACTCTGT CCAACTTCTA TGTATTTAAC AATTAGACCA 
AAAAACTACA TCTCTAACCT ATATTTCTTT TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_021476194.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331869.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X3, mRNA.

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CloneID	ODa142651
Clone ID Related Accession (Same CDS sequence)	XM_021476194.1
Accession Version	XM_021476194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGC ATTCAGCAAT CCCATCAATA CAGACAATTT TGAAAACAAC ACTGGCCGAA 
ATGCAGCCCC AAACCAGGAC AAACCAGAGA CTCCAACATG TTTCACTGAA GGCTATTGAG 
GAATCTGTGC GCTCCATGGT CATGCGCTAC GGCAGTCGCT TGTGGAAGCT CCGTGTGCTT 
CAAGCCGAGC TGAAGATCAA CATCCGATTG ACTCCCACCG GCAAACAGAT CCCCATCCGC 
CTCTTCCTGA CCAACGAGAG TGGCTACTAC CTGGACATCA GCCTGTACAA GGAAGTCACT 
GACTCCCGTA CAGGACAGAT CATGTTCCAG GCATATGGAG ATAAGCAGGG TCCTCTTCAT 
GGCATGCTCA TCAACACACC CTACGTCACA AAAGACCTGC TGCAGTCCAA ACGCTTCCAG 
GCACAGAGCC TCGGCACTAC CTATGTTTAC GACTTCCCAG AAATGTTTCG CCAGGCTTTA 
AGGAAGCTGT GGCAATCCAT GGATGCACAC GCCAACTTGC CCAAGTGCCC TCTTCCATCA 
GAACTGCTCA CCTTCACAGA GCTGGTTCTG GATTCTCAGG GTCAGCTGGT GCAGATGAAC 
CGCCTACCTG GAGGGAATGA GATTGGCATG GTAGCATGGC GAATGACCCT CCGGACCCCT 
GAGTATCCAG CCGGCCGTGA GATTATAGTG ATCAGCAATG ACATCACACA TAAGATTGGC 
TCGTTTGGGC CACAAGAAGA TGTTCTGTTC CAGCAAGCTT CAGAAATGGC AAGAGAAAGT 
GGCATTCCAC GGATCTATAT TGCTGCCAAC AGCGGAGCTC GTATAGGCCT GGCAGAGGAG 
ATCAGACACA TGTTTCATGT GGCCTGGCAG GATCCGGTGG ACCCATACAA GGGTTTTAAG 
TACCTATACC TCACACCACA GGACTATAAG AAGGTGTCTG CTCTGAACTC AGTCCACTGT 
GAACATGTGG AGGATGAGGG GGAATCCAGG TATAAAATCA CTGATATCAT TGGGAAAGAG 
GAAGGGTTGG GTGTGGAGAA CTTGAGAGGT TCTGGCATGA TCGCAGGTGA ATCGTCACTG 
GCGTATGAGG ATATTATCAC CATGAATCTG GTGACATGCA GGGCCATTGG AATCGGAGCG 
TACTTGGTGA GACTGGGCCA AAGAACCATA CAGGTGGAAA ACTCTCATAT CATCCTGACT 
GGAGCCGGAG CTCTCAATAA GGTTCTCGGG CGTGAGGTGT ACACTTCCAA TAACCAACTG 
GGTGGAGTAC AGATCATGCA CAATAACGGA GTCACACACA CCAATGTGTG CGATGATTTT 
GAGGGAGTGT ATACATTGCT GCACTGGCTG TCTTACATGC CAAAGAATAT GTCCAGCCCT 
GTGCCGATAC TCTCTGCTAA AGATCCCATT GATCGAGCGA TAGAGTTTGT CCCAACCAAG 
GCACCTTATG ACCCACGCTG GATGCTAGCT GGACGTCCCA ATCAGAACAT TAAGGGTGCA 
TGGGTGAGTG GGTTCTTCGA CCAGGGCTCT TTTCTGGAGA TCATGCAGCC ATGGGCACAG 
AGTGTTGTCG TAGGACGGGC CAGGTTGGGT GGGATCCCAA CTGGAGTAGT TGCCGTGGAA 
ACCCGATCAG TGGAGCTTTC AATCCCAGCA GACCCAGCTA ATCTGGACTC GGAAGCCAAG 
ATAATCCAGC AGGCTGGGCA AGTGTGGTTC CCTGATTCTG CTTTCAAAAC TGCACAAGCC 
ATTAAAGACT TCAATAGAGA GGGACTTCCG CTTATAGTGT TTGCCAACTG GAGAGGTTTT 
TCTGGAGGCA TGAAAGACAT GTATGACCAG GTGCTGAAGT TTGGAGCGTA TATAGTGGAC 
GGTCTGAGAG AGTACAGACA GCCGGTTCTG GTGTATATCC CACCACAGGC GGAGCTCCGG 
GGAGGATCAT GGGTTGTCAT AGACCCTACT ATCAATCCTC GCCACATGGA AATGTATGCA 
GACAAAGACA GCCGTGGCGG TGTGCTGGAG CCTGAAGGCA CAGTGGAGAT CAAGTTCCGC 
AAAAAAGACC TGGTGAAGAC CATGCGACGG GTGGACCCTG TGTACATGGG GCTTGCAGAA 
AGACTGGGAA CCCCAGAGTT GAGTGTGTCT GAGCGTAAAG AGCTGGAGAG TAAACTAAAA 
GAGAGAGAAG AGTTCCTGCT GCCCATATAT CATCAAGTAG CTGTTCAGTT CGCTGACCTT 
CATGACACAC CGGGCCGCAT GCAGGAGAAA GGAGTCATCA CTGATATCCT TGAGTGGAGC 
ACGTCTCGGC AGTTTTTCTA CTGGCGTTTG CGGAGGTTAT TGCTGGAGGA AACGGTGAAG 
AGGAAGATCC AGTGTGCTAA CAGTGAGCTG ACGGACGGAC AGGTTCAGGC TATGCTTCGC 
CGTTGGTTCG TGGAAGCAGA GGGAGCTGTG AAAGCGTATT TGTGGGACAG TAATGAAGAT 
GTGGTGGAGT GGTTGGAGAG GCAGCTGGCA GAGGAAGAAG GCGCCAGATC CGTCATCGAC 
GAGAATATCA AATATATTCG CAGAGATCAC ATCCTCAAAC AGATCCGCAG CCTGGTTCAA 
GCTAACCCTG AGGTGGCTAT GGACTCCATT GTACACATGA CCCAGCACAT TTCCCCCACA 
CAGCGCGCCG AAGTCGTCCG CATCCTCTCC ACCATGGACA CCCCAGCCTC CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331869.1
CDS	237..7493
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRKLSAPLQDYKPPANTANTSGATEAASDSAESEDRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKHSAIPSIQTILKTTLAEMQPQTRTNQRLQHVSLKAIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_021476194.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476194.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGC ATTCAGCAAT CCCATCAATA CAGACAATTT TGAAAACAAC ACTGGCCGAA 
ATGCAGCCCC AAACCAGGAC AAACCAGAGA CTCCAACATG TTTCACTGAA GGCTATTGAG 
GAATCTGTGC GCTCCATGGT CATGCGCTAC GGCAGTCGCT TGTGGAAGCT CCGTGTGCTT 
CAAGCCGAGC TGAAGATCAA CATCCGATTG ACTCCCACCG GCAAACAGAT CCCCATCCGC 
CTCTTCCTGA CCAACGAGAG TGGCTACTAC CTGGACATCA GCCTGTACAA GGAAGTCACT 
GACTCCCGTA CAGGACAGAT CATGTTCCAG GCATATGGAG ATAAGCAGGG TCCTCTTCAT 
GGCATGCTCA TCAACACACC CTACGTCACA AAAGACCTGC TGCAGTCCAA ACGCTTCCAG 
GCACAGAGCC TCGGCACTAC CTATGTTTAC GACTTCCCAG AAATGTTTCG CCAGGCTTTA 
AGGAAGCTGT GGCAATCCAT GGATGCACAC GCCAACTTGC CCAAGTGCCC TCTTCCATCA 
GAACTGCTCA CCTTCACAGA GCTGGTTCTG GATTCTCAGG GTCAGCTGGT GCAGATGAAC 
CGCCTACCTG GAGGGAATGA GATTGGCATG GTAGCATGGC GAATGACCCT CCGGACCCCT 
GAGTATCCAG CCGGCCGTGA GATTATAGTG ATCAGCAATG ACATCACACA TAAGATTGGC 
TCGTTTGGGC CACAAGAAGA TGTTCTGTTC CAGCAAGCTT CAGAAATGGC AAGAGAAAGT 
GGCATTCCAC GGATCTATAT TGCTGCCAAC AGCGGAGCTC GTATAGGCCT GGCAGAGGAG 
ATCAGACACA TGTTTCATGT GGCCTGGCAG GATCCGGTGG ACCCATACAA GGGTTTTAAG 
TACCTATACC TCACACCACA GGACTATAAG AAGGTGTCTG CTCTGAACTC AGTCCACTGT 
GAACATGTGG AGGATGAGGG GGAATCCAGG TATAAAATCA CTGATATCAT TGGGAAAGAG 
GAAGGGTTGG GTGTGGAGAA CTTGAGAGGT TCTGGCATGA TCGCAGGTGA ATCGTCACTG 
GCGTATGAGG ATATTATCAC CATGAATCTG GTGACATGCA GGGCCATTGG AATCGGAGCG 
TACTTGGTGA GACTGGGCCA AAGAACCATA CAGGTGGAAA ACTCTCATAT CATCCTGACT 
GGAGCCGGAG CTCTCAATAA GGTTCTCGGG CGTGAGGTGT ACACTTCCAA TAACCAACTG 
GGTGGAGTAC AGATCATGCA CAATAACGGA GTCACACACA CCAATGTGTG CGATGATTTT 
GAGGGAGTGT ATACATTGCT GCACTGGCTG TCTTACATGC CAAAGAATAT GTCCAGCCCT 
GTGCCGATAC TCTCTGCTAA AGATCCCATT GATCGAGCGA TAGAGTTTGT CCCAACCAAG 
GCACCTTATG ACCCACGCTG GATGCTAGCT GGACGTCCCA ATCAGAACAT TAAGGGTGCA 
TGGGTGAGTG GGTTCTTCGA CCAGGGCTCT TTTCTGGAGA TCATGCAGCC ATGGGCACAG 
AGTGTTGTCG TAGGACGGGC CAGGTTGGGT GGGATCCCAA CTGGAGTAGT TGCCGTGGAA 
ACCCGATCAG TGGAGCTTTC AATCCCAGCA GACCCAGCTA ATCTGGACTC GGAAGCCAAG 
ATAATCCAGC AGGCTGGGCA AGTGTGGTTC CCTGATTCTG CTTTCAAAAC TGCACAAGCC 
ATTAAAGACT TCAATAGAGA GGGACTTCCG CTTATAGTGT TTGCCAACTG GAGAGGTTTT 
TCTGGAGGCA TGAAAGACAT GTATGACCAG GTGCTGAAGT TTGGAGCGTA TATAGTGGAC 
GGTCTGAGAG AGTACAGACA GCCGGTTCTG GTGTATATCC CACCACAGGC GGAGCTCCGG 
GGAGGATCAT GGGTTGTCAT AGACCCTACT ATCAATCCTC GCCACATGGA AATGTATGCA 
GACAAAGACA GCCGTGGCGG TGTGCTGGAG CCTGAAGGCA CAGTGGAGAT CAAGTTCCGC 
AAAAAAGACC TGGTGAAGAC CATGCGACGG GTGGACCCTG TGTACATGGG GCTTGCAGAA 
AGACTGGGAA CCCCAGAGTT GAGTGTGTCT GAGCGTAAAG AGCTGGAGAG TAAACTAAAA 
GAGAGAGAAG AGTTCCTGCT GCCCATATAT CATCAAGTAG CTGTTCAGTT CGCTGACCTT 
CATGACACAC CGGGCCGCAT GCAGGAGAAA GGAGTCATCA CTGATATCCT TGAGTGGAGC 
ACGTCTCGGC AGTTTTTCTA CTGGCGTTTG CGGAGGTTAT TGCTGGAGGA AACGGTGAAG 
AGGAAGATCC AGTGTGCTAA CAGTGAGCTG ACGGACGGAC AGGTTCAGGC TATGCTTCGC 
CGTTGGTTCG TGGAAGCAGA GGGAGCTGTG AAAGCGTATT TGTGGGACAG TAATGAAGAT 
GTGGTGGAGT GGTTGGAGAG GCAGCTGGCA GAGGAAGAAG GCGCCAGATC CGTCATCGAC 
GAGAATATCA AATATATTCG CAGAGATCAC ATCCTCAAAC AGATCCGCAG CCTGGTTCAA 
GCTAACCCTG AGGTGGCTAT GGACTCCATT GTACACATGA CCCAGCACAT TTCCCCCACA 
CAGCGCGCCG AAGTCGTCCG CATCCTCTCC ACCATGGACA CCCCAGCCTC CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_021476195.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331870.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X4, mRNA.

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CloneID	ODa142652
Clone ID Related Accession (Same CDS sequence)	XM_021476195.1
Accession Version	XM_021476195.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG AGAGAATTCC CCAAATTTTT TACATTCCGG 
GCTCGAGACA AGTTTGAGGA AGACCGTATC TACAGACACT TGGAACCTGC ATTAGCTTTC 
CAGCTGGAGC TCAACCGCAT GCGTAATTTT GCACTGACAG CCATCCCCTG TGCCAATCAT 
AAGATGCACC TGTACTTGGG AGCTGCCCGT GTGGAAGTAG GCACTGAGGT CACAGATTAC 
CGCTTCTTTG TGCGGGCAAT CATCCGTCAC TCAGATTTGG TCACCAAGGA AGCATCATTT 
GAGTACCTGC ACAACGAGGC AGAACGCTTG CTGCTGGAGG CCATGGATGA ACTGGAAGTG 
GCTTTCAACA ACACCACTGT GCGAACCGAC TGCAATCACA TCTTCCTCAA CTTCGTTCCA 
ACAGTCATCA TGGACCCTTC AAAGCATTCA GCAATCCCAT CAATACAGAC AATTTTGAAA 
ACAACACTGG CCGAAATGCA GCCCCAAACC AGGACAAACC AGAGACTCCA ACATGTTTCA 
CTGAAGGCTA TTGAGGAATC TGTGCGCTCC ATGGTCATGC GCTACGGCAG TCGCTTGTGG 
AAGCTCCGTG TGCTTCAAGC CGAGCTGAAG ATCAACATCC GATTGACTCC CACCGGCAAA 
CAGATCCCCA TCCGCCTCTT CCTGACCAAC GAGAGTGGCT ACTACCTGGA CATCAGCCTG 
TACAAGGAAG TCACTGACTC CCGTACAGGA CAGGTGGGGC ACAAAGACCG ACAGATCATG 
TTCCAGGCAT ATGGAGATAA GCAGGGTCCT CTTCATGGCA TGCTCATCAA CACACCCTAC 
GTCACAAAAG ACCTGCTGCA GTCCAAACGC TTCCAGGCAC AGAGCCTCGG CACTACCTAT 
GTTTACGACT TCCCAGAAAT GTTTCGCCAG GCTTTAAGGA AGCTGTGGCA ATCCATGGAT 
GCACACGCCA ACTTGCCCAA GTGCCCTCTT CCATCAGAAC TGCTCACCTT CACAGAGCTG 
GTTCTGGATT CTCAGGGTCA GCTGGTGCAG ATGAACCGCC TACCTGGAGG GAATGAGATT 
GGCATGGTAG CATGGCGAAT GACCCTCCGG ACCCCTGAGT ATCCAGCCGG CCGTGAGATT 
ATAGTGATCA GCAATGACAT CACACATAAG ATTGGCTCGT TTGGGCCACA AGAAGATGTT 
CTGTTCCAGC AAGCTTCAGA AATGGCAAGA GAAAGTGGCA TTCCACGGAT CTATATTGCT 
GCCAACAGCG GAGCTCGTAT AGGCCTGGCA GAGGAGATCA GACACATGTT TCATGTGGCC 
TGGCAGGATC CGGTGGACCC ATACAAGGGT TTTAAGTACC TATACCTCAC ACCACAGGAC 
TATAAGAAGG TGTCTGCTCT GAACTCAGTC CACTGTGAAC ATGTGGAGGA TGAGGGGGAA 
TCCAGGTATA AAATCACTGA TATCATTGGG AAAGAGGAAG GGTTGGGTGT GGAGAACTTG 
AGAGGTTCTG GCATGATCGC AGGTGAATCG TCACTGGCGT ATGAGGATAT TATCACCATG 
AATCTGGTGA CATGCAGGGC CATTGGAATC GGAGCGTACT TGGTGAGACT GGGCCAAAGA 
ACCATACAGG TGGAAAACTC TCATATCATC CTGACTGGAG CCGGAGCTCT CAATAAGGTT 
CTCGGGCGTG AGGTGTACAC TTCCAATAAC CAACTGGGTG GAGTACAGAT CATGCACAAT 
AACGGAGTCA CACACACCAA TGTGTGCGAT GATTTTGAGG GAGTGTATAC ATTGCTGCAC 
TGGCTGTCTT ACATGCCAAA GAATATGTCC AGCCCTGTGC CGATACTCTC TGCTAAAGAT 
CCCATTGATC GAGCGATAGA GTTTGTCCCA ACCAAGGCAC CTTATGACCC ACGCTGGATG 
CTAGCTGGAC GTCCCAATCA GAACATTAAG GGTGCATGGG TGAGTGGGTT CTTCGACCAG 
GGCTCTTTTC TGGAGATCAT GCAGCCATGG GCACAGAGTG TTGTCGTAGG ACGGGCCAGG 
TTGGGTGGGA TCCCAACTGG AGTAGTTGCC GTGGAAACCC GATCAGTGGA GCTTTCAATC 
CCAGCAGACC CAGCTAATCT GGACTCGGAA GCCAAGATAA TCCAGCAGGC TGGGCAAGTG 
TGGTTCCCTG ATTCTGCTTT CAAAACTGCA CAAGCCATTA AAGACTTCAA TAGAGAGGGA 
CTTCCGCTTA TAGTGTTTGC CAACTGGAGA GGTTTTTCTG GAGGCATGAA AGACATGTAT 
GACCAGGTGC TGAAGTTTGG AGCGTATATA GTGGACGGTC TGAGAGAGTA CAGACAGCCG 
GTTCTGGTGT ATATCCCACC ACAGGCGGAG CTCCGGGGAG GATCATGGGT TGTCATAGAC 
CCTACTATCA ATCCTCGCCA CATGGAAATG TATGCAGACA AAGACAGCCG TGGCGGTGTG 
CTGGAGCCTG AAGGCACAGT GGAGATCAAG TTCCGCAAAA AAGACCTGGT GAAGACCATG 
CGACGGGTGG ACCCTGTGTA CATGGGGCTT GCAGAAAGAC TGGGAACCCC AGAGTTGAGT 
GTGTCTGAGC GTAAAGAGCT GGAGAGTAAA CTAAAAGAGA GAGAAGAGTT CCTGCTGCCC 
ATATATCATC AAGTAGCTGT TCAGTTCGCT GACCTTCATG ACACACCGGG CCGCATGCAG 
GAGAAAGGAG TCATCACTGA TATCCTTGAG TGGAGCACGT CTCGGCAGTT TTTCTACTGG 
CGTTTGCGGA GGTTATTGCT GGAGGAAACG GTGAAGAGGA AGATCCAGTG TGCTAACAGT 
GAGCTGACGG ACGGACAGGT TCAGGCTATG CTTCGCCGTT GGTTCGTGGA AGCAGAGGGA 
GCTGTGAAAG CGTATTTGTG GGACAGTAAT GAAGATGTGG TGGAGTGGTT GGAGAGGCAG 
CTGGCAGAGG AAGAAGGCGC CAGATCCGTC ATCGACGAGA ATATCAAATA TATTCGCAGA 
GATCACATCC TCAAACAGAT CCGCAGCCTG GTTCAAGCTA ACCCTGAGGT GGCTATGGAC 
TCCATTGTAC ACATGACCCA GCACATTTCC CCCACACAGC GCGCCGAAGT CGTCCGCATC 
CTCTCCACCA TGGACACCCC AGCCTCCTCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331870.1
CDS	237..7469
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRKLSAPLQDYKPPANTANTSGATEAASDSAESEDRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKHSAIPSIQTILKTTLAEMQPQTRTNQRLQHVSLKAIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_021476195.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476195.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG AGAGAATTCC CCAAATTTTT TACATTCCGG 
GCTCGAGACA AGTTTGAGGA AGACCGTATC TACAGACACT TGGAACCTGC ATTAGCTTTC 
CAGCTGGAGC TCAACCGCAT GCGTAATTTT GCACTGACAG CCATCCCCTG TGCCAATCAT 
AAGATGCACC TGTACTTGGG AGCTGCCCGT GTGGAAGTAG GCACTGAGGT CACAGATTAC 
CGCTTCTTTG TGCGGGCAAT CATCCGTCAC TCAGATTTGG TCACCAAGGA AGCATCATTT 
GAGTACCTGC ACAACGAGGC AGAACGCTTG CTGCTGGAGG CCATGGATGA ACTGGAAGTG 
GCTTTCAACA ACACCACTGT GCGAACCGAC TGCAATCACA TCTTCCTCAA CTTCGTTCCA 
ACAGTCATCA TGGACCCTTC AAAGCATTCA GCAATCCCAT CAATACAGAC AATTTTGAAA 
ACAACACTGG CCGAAATGCA GCCCCAAACC AGGACAAACC AGAGACTCCA ACATGTTTCA 
CTGAAGGCTA TTGAGGAATC TGTGCGCTCC ATGGTCATGC GCTACGGCAG TCGCTTGTGG 
AAGCTCCGTG TGCTTCAAGC CGAGCTGAAG ATCAACATCC GATTGACTCC CACCGGCAAA 
CAGATCCCCA TCCGCCTCTT CCTGACCAAC GAGAGTGGCT ACTACCTGGA CATCAGCCTG 
TACAAGGAAG TCACTGACTC CCGTACAGGA CAGGTGGGGC ACAAAGACCG ACAGATCATG 
TTCCAGGCAT ATGGAGATAA GCAGGGTCCT CTTCATGGCA TGCTCATCAA CACACCCTAC 
GTCACAAAAG ACCTGCTGCA GTCCAAACGC TTCCAGGCAC AGAGCCTCGG CACTACCTAT 
GTTTACGACT TCCCAGAAAT GTTTCGCCAG GCTTTAAGGA AGCTGTGGCA ATCCATGGAT 
GCACACGCCA ACTTGCCCAA GTGCCCTCTT CCATCAGAAC TGCTCACCTT CACAGAGCTG 
GTTCTGGATT CTCAGGGTCA GCTGGTGCAG ATGAACCGCC TACCTGGAGG GAATGAGATT 
GGCATGGTAG CATGGCGAAT GACCCTCCGG ACCCCTGAGT ATCCAGCCGG CCGTGAGATT 
ATAGTGATCA GCAATGACAT CACACATAAG ATTGGCTCGT TTGGGCCACA AGAAGATGTT 
CTGTTCCAGC AAGCTTCAGA AATGGCAAGA GAAAGTGGCA TTCCACGGAT CTATATTGCT 
GCCAACAGCG GAGCTCGTAT AGGCCTGGCA GAGGAGATCA GACACATGTT TCATGTGGCC 
TGGCAGGATC CGGTGGACCC ATACAAGGGT TTTAAGTACC TATACCTCAC ACCACAGGAC 
TATAAGAAGG TGTCTGCTCT GAACTCAGTC CACTGTGAAC ATGTGGAGGA TGAGGGGGAA 
TCCAGGTATA AAATCACTGA TATCATTGGG AAAGAGGAAG GGTTGGGTGT GGAGAACTTG 
AGAGGTTCTG GCATGATCGC AGGTGAATCG TCACTGGCGT ATGAGGATAT TATCACCATG 
AATCTGGTGA CATGCAGGGC CATTGGAATC GGAGCGTACT TGGTGAGACT GGGCCAAAGA 
ACCATACAGG TGGAAAACTC TCATATCATC CTGACTGGAG CCGGAGCTCT CAATAAGGTT 
CTCGGGCGTG AGGTGTACAC TTCCAATAAC CAACTGGGTG GAGTACAGAT CATGCACAAT 
AACGGAGTCA CACACACCAA TGTGTGCGAT GATTTTGAGG GAGTGTATAC ATTGCTGCAC 
TGGCTGTCTT ACATGCCAAA GAATATGTCC AGCCCTGTGC CGATACTCTC TGCTAAAGAT 
CCCATTGATC GAGCGATAGA GTTTGTCCCA ACCAAGGCAC CTTATGACCC ACGCTGGATG 
CTAGCTGGAC GTCCCAATCA GAACATTAAG GGTGCATGGG TGAGTGGGTT CTTCGACCAG 
GGCTCTTTTC TGGAGATCAT GCAGCCATGG GCACAGAGTG TTGTCGTAGG ACGGGCCAGG 
TTGGGTGGGA TCCCAACTGG AGTAGTTGCC GTGGAAACCC GATCAGTGGA GCTTTCAATC 
CCAGCAGACC CAGCTAATCT GGACTCGGAA GCCAAGATAA TCCAGCAGGC TGGGCAAGTG 
TGGTTCCCTG ATTCTGCTTT CAAAACTGCA CAAGCCATTA AAGACTTCAA TAGAGAGGGA 
CTTCCGCTTA TAGTGTTTGC CAACTGGAGA GGTTTTTCTG GAGGCATGAA AGACATGTAT 
GACCAGGTGC TGAAGTTTGG AGCGTATATA GTGGACGGTC TGAGAGAGTA CAGACAGCCG 
GTTCTGGTGT ATATCCCACC ACAGGCGGAG CTCCGGGGAG GATCATGGGT TGTCATAGAC 
CCTACTATCA ATCCTCGCCA CATGGAAATG TATGCAGACA AAGACAGCCG TGGCGGTGTG 
CTGGAGCCTG AAGGCACAGT GGAGATCAAG TTCCGCAAAA AAGACCTGGT GAAGACCATG 
CGACGGGTGG ACCCTGTGTA CATGGGGCTT GCAGAAAGAC TGGGAACCCC AGAGTTGAGT 
GTGTCTGAGC GTAAAGAGCT GGAGAGTAAA CTAAAAGAGA GAGAAGAGTT CCTGCTGCCC 
ATATATCATC AAGTAGCTGT TCAGTTCGCT GACCTTCATG ACACACCGGG CCGCATGCAG 
GAGAAAGGAG TCATCACTGA TATCCTTGAG TGGAGCACGT CTCGGCAGTT TTTCTACTGG 
CGTTTGCGGA GGTTATTGCT GGAGGAAACG GTGAAGAGGA AGATCCAGTG TGCTAACAGT 
GAGCTGACGG ACGGACAGGT TCAGGCTATG CTTCGCCGTT GGTTCGTGGA AGCAGAGGGA 
GCTGTGAAAG CGTATTTGTG GGACAGTAAT GAAGATGTGG TGGAGTGGTT GGAGAGGCAG 
CTGGCAGAGG AAGAAGGCGC CAGATCCGTC ATCGACGAGA ATATCAAATA TATTCGCAGA 
GATCACATCC TCAAACAGAT CCGCAGCCTG GTTCAAGCTA ACCCTGAGGT GGCTATGGAC 
TCCATTGTAC ACATGACCCA GCACATTTCC CCCACACAGC GCGCCGAAGT CGTCCGCATC 
CTCTCCACCA TGGACACCCC AGCCTCCTCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_021476196.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331871.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X5, mRNA.

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CloneID	ODa142653
Clone ID Related Accession (Same CDS sequence)	XM_021476196.1
Accession Version	XM_021476196.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGAATG 
TCCTTTTCCT CAAACCTGAA CCACTACGGC ATGGTCCACG TGGCCAGCGT TAGTGATGTG 
CTCCTAGACA CCTCCTTTAC CCCACCCTGT CAGCGCATGG GAGCCATGGT TTCCTTCCGC 
TCTTTCCAGG AGTTCACCAG GAACATAAAG GATGTTCTGA GCTGTTTCTC AGACTCTCCT 
CCATCAACTC CAACCTTTCC TGAAGGGGGG AACCCTGTGC TGTATAGAGA GGAGGACAGC 
AAGAGCATTC AAGATGAGCC CATTCATATT CTTAATGTGG CAATAAAGAC GGACAGTGAT 
ATTGATGATG ATGGGCTGGC GGCCATGTTT CGTGAGTTTA CACAGTCTAA GAAATCTCTG 
CTGTTTGACC ACGGCATCCG AAGACTTACC TTCCTGGTTG CTCAGAAGGA TTTCAGAAAG 
CAAATCAACT GTGAGGTGGA CCAGAGGTTC CATAGAGAAT TCCCCAAATT TTTTACATTC 
CGGGCTCGAG ACAAGTTTGA GGAAGACCGT ATCTACAGAC ACTTGGAACC TGCATTAGCT 
TTCCAGCTGG AGCTCAACCG CATGCGTAAT TTTGCACTGA CAGCCATCCC CTGTGCCAAT 
CATAAGATGC ACCTGTACTT GGGAGCTGCC CGTGTGGAAG TAGGCACTGA GGTCACAGAT 
TACCGCTTCT TTGTGCGGGC AATCATCCGT CACTCAGATT TGGTCACCAA GGAAGCATCA 
TTTGAGTACC TGCACAACGA GGCAGAACGC TTGCTGCTGG AGGCCATGGA TGAACTGGAA 
GTGGCTTTCA ACAACACCAC TGTGCGAACC GACTGCAATC ACATCTTCCT CAACTTCGTT 
CCAACAGTCA TCATGGACCC TTCAAAGCAT TCAGCAATCC CATCAATACA GACAATTTTG 
AAAACAACAC TGGCCGAAAT GCAGCCCCAA ACCAGGACAA ACCAGAGACT CCAACATGTT 
TCACTGAAGG CTATTGAGGA ATCTGTGCGC TCCATGGTCA TGCGCTACGG CAGTCGCTTG 
TGGAAGCTCC GTGTGCTTCA AGCCGAGCTG AAGATCAACA TCCGATTGAC TCCCACCGGC 
AAACAGATCC CCATCCGCCT CTTCCTGACC AACGAGAGTG GCTACTACCT GGACATCAGC 
CTGTACAAGG AAGTCACTGA CTCCCGTACA GGACAGGTGG GGCACAAAGA CCGACAGATC 
ATGTTCCAGG CATATGGAGA TAAGCAGGGT CCTCTTCATG GCATGCTCAT CAACACACCC 
TACGTCACAA AAGACCTGCT GCAGTCCAAA CGCTTCCAGG CACAGAGCCT CGGCACTACC 
TATGTTTACG ACTTCCCAGA AATGTTTCGC CAGGCTTTAA GGAAGCTGTG GCAATCCATG 
GATGCACACG CCAACTTGCC CAAGTGCCCT CTTCCATCAG AACTGCTCAC CTTCACAGAG 
CTGGTTCTGG ATTCTCAGGG TCAGCTGGTG CAGATGAACC GCCTACCTGG AGGGAATGAG 
ATTGGCATGG TAGCATGGCG AATGACCCTC CGGACCCCTG AGTATCCAGC CGGCCGTGAG 
ATTATAGTGA TCAGCAATGA CATCACACAT AAGATTGGCT CGTTTGGGCC ACAAGAAGAT 
GTTCTGTTCC AGCAAGCTTC AGAAATGGCA AGAGAAAGTG GCATTCCACG GATCTATATT 
GCTGCCAACA GCGGAGCTCG TATAGGCCTG GCAGAGGAGA TCAGACACAT GTTTCATGTG 
GCCTGGCAGG ATCCGGTGGA CCCATACAAG GGTTTTAAGT ACCTATACCT CACACCACAG 
GACTATAAGA AGGTGTCTGC TCTGAACTCA GTCCACTGTG AACATGTGGA GGATGAGGGG 
GAATCCAGGT ATAAAATCAC TGATATCATT GGGAAAGAGG AAGGGTTGGG TGTGGAGAAC 
TTGAGAGGTT CTGGCATGAT CGCAGGTGAA TCGTCACTGG CGTATGAGGA TATTATCACC 
ATGAATCTGG TGACATGCAG GGCCATTGGA ATCGGAGCGT ACTTGGTGAG ACTGGGCCAA 
AGAACCATAC AGGTGGAAAA CTCTCATATC ATCCTGACTG GAGCCGGAGC TCTCAATAAG 
GTTCTCGGGC GTGAGGTGTA CACTTCCAAT AACCAACTGG GTGGAGTACA GATCATGCAC 
AATAACGGAG TCACACACAC CAATGTGTGC GATGATTTTG AGGGAGTGTA TACATTGCTG 
CACTGGCTGT CTTACATGCC AAAGAATATG TCCAGCCCTG TGCCGATACT CTCTGCTAAA 
GATCCCATTG ATCGAGCGAT AGAGTTTGTC CCAACCAAGG CACCTTATGA CCCACGCTGG 
ATGCTAGCTG GACGTCCCAA TCAGAACATT AAGGGTGCAT GGGTGAGTGG GTTCTTCGAC 
CAGGGCTCTT TTCTGGAGAT CATGCAGCCA TGGGCACAGA GTGTTGTCGT AGGACGGGCC 
AGGTTGGGTG GGATCCCAAC TGGAGTAGTT GCCGTGGAAA CCCGATCAGT GGAGCTTTCA 
ATCCCAGCAG ACCCAGCTAA TCTGGACTCG GAAGCCAAGA TAATCCAGCA GGCTGGGCAA 
GTGTGGTTCC CTGATTCTGC TTTCAAAACT GCACAAGCCA TTAAAGACTT CAATAGAGAG 
GGACTTCCGC TTATAGTGTT TGCCAACTGG AGAGGTTTTT CTGGAGGCAT GAAAGACATG 
TATGACCAGG TGCTGAAGTT TGGAGCGTAT ATAGTGGACG GTCTGAGAGA GTACAGACAG 
CCGGTTCTGG TGTATATCCC ACCACAGGCG GAGCTCCGGG GAGGATCATG GGTTGTCATA 
GACCCTACTA TCAATCCTCG CCACATGGAA ATGTATGCAG ACAAAGACAG CCGTGGCGGT 
GTGCTGGAGC CTGAAGGCAC AGTGGAGATC AAGTTCCGCA AAAAAGACCT GGTGAAGACC 
ATGCGACGGG TGGACCCTGT GTACATGGGG CTTGCAGAAA GACTGGGAAC CCCAGAGTTG 
AGTGTGTCTG AGCGTAAAGA GCTGGAGAGT AAACTAAAAG AGAGAGAAGA GTTCCTGCTG 
CCCATATATC ATCAAGTAGC TGTTCAGTTC GCTGACCTTC ATGACACACC GGGCCGCATG 
CAGGAGAAAG GAGTCATCAC TGATATCCTT GAGTGGAGCA CGTCTCGGCA GTTTTTCTAC 
TGGCGTTTGC GGAGGTTATT GCTGGAGGAA ACGGTGAAGA GGAAGATCCA GTGTGCTAAC 
AGTGAGCTGA CGGACGGACA GGTTCAGGCT ATGCTTCGCC GTTGGTTCGT GGAAGCAGAG 
GGAGCTGTGA AAGCGTATTT GTGGGACAGT AATGAAGATG TGGTGGAGTG GTTGGAGAGG 
CAGCTGGCAG AGGAAGAAGG CGCCAGATCC GTCATCGACG AGAATATCAA ATATATTCGC 
AGAGATCACA TCCTCAAACA GATCCGCAGC CTGGTTCAAG CTAACCCTGA GGTGGCTATG 
GACTCCATTG TACACATGAC CCAGCACATT TCCCCCACAC AGCGCGCCGA AGTCGTCCGC 
ATCCTCTCCA CCATGGACAC CCCAGCCTCC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331871.1
CDS	237..7412
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKHSAIPSIQTILKTTLAEMQPQTRTNQRLQHVSLKAIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_021476196.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476196.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGAATG 
TCCTTTTCCT CAAACCTGAA CCACTACGGC ATGGTCCACG TGGCCAGCGT TAGTGATGTG 
CTCCTAGACA CCTCCTTTAC CCCACCCTGT CAGCGCATGG GAGCCATGGT TTCCTTCCGC 
TCTTTCCAGG AGTTCACCAG GAACATAAAG GATGTTCTGA GCTGTTTCTC AGACTCTCCT 
CCATCAACTC CAACCTTTCC TGAAGGGGGG AACCCTGTGC TGTATAGAGA GGAGGACAGC 
AAGAGCATTC AAGATGAGCC CATTCATATT CTTAATGTGG CAATAAAGAC GGACAGTGAT 
ATTGATGATG ATGGGCTGGC GGCCATGTTT CGTGAGTTTA CACAGTCTAA GAAATCTCTG 
CTGTTTGACC ACGGCATCCG AAGACTTACC TTCCTGGTTG CTCAGAAGGA TTTCAGAAAG 
CAAATCAACT GTGAGGTGGA CCAGAGGTTC CATAGAGAAT TCCCCAAATT TTTTACATTC 
CGGGCTCGAG ACAAGTTTGA GGAAGACCGT ATCTACAGAC ACTTGGAACC TGCATTAGCT 
TTCCAGCTGG AGCTCAACCG CATGCGTAAT TTTGCACTGA CAGCCATCCC CTGTGCCAAT 
CATAAGATGC ACCTGTACTT GGGAGCTGCC CGTGTGGAAG TAGGCACTGA GGTCACAGAT 
TACCGCTTCT TTGTGCGGGC AATCATCCGT CACTCAGATT TGGTCACCAA GGAAGCATCA 
TTTGAGTACC TGCACAACGA GGCAGAACGC TTGCTGCTGG AGGCCATGGA TGAACTGGAA 
GTGGCTTTCA ACAACACCAC TGTGCGAACC GACTGCAATC ACATCTTCCT CAACTTCGTT 
CCAACAGTCA TCATGGACCC TTCAAAGCAT TCAGCAATCC CATCAATACA GACAATTTTG 
AAAACAACAC TGGCCGAAAT GCAGCCCCAA ACCAGGACAA ACCAGAGACT CCAACATGTT 
TCACTGAAGG CTATTGAGGA ATCTGTGCGC TCCATGGTCA TGCGCTACGG CAGTCGCTTG 
TGGAAGCTCC GTGTGCTTCA AGCCGAGCTG AAGATCAACA TCCGATTGAC TCCCACCGGC 
AAACAGATCC CCATCCGCCT CTTCCTGACC AACGAGAGTG GCTACTACCT GGACATCAGC 
CTGTACAAGG AAGTCACTGA CTCCCGTACA GGACAGGTGG GGCACAAAGA CCGACAGATC 
ATGTTCCAGG CATATGGAGA TAAGCAGGGT CCTCTTCATG GCATGCTCAT CAACACACCC 
TACGTCACAA AAGACCTGCT GCAGTCCAAA CGCTTCCAGG CACAGAGCCT CGGCACTACC 
TATGTTTACG ACTTCCCAGA AATGTTTCGC CAGGCTTTAA GGAAGCTGTG GCAATCCATG 
GATGCACACG CCAACTTGCC CAAGTGCCCT CTTCCATCAG AACTGCTCAC CTTCACAGAG 
CTGGTTCTGG ATTCTCAGGG TCAGCTGGTG CAGATGAACC GCCTACCTGG AGGGAATGAG 
ATTGGCATGG TAGCATGGCG AATGACCCTC CGGACCCCTG AGTATCCAGC CGGCCGTGAG 
ATTATAGTGA TCAGCAATGA CATCACACAT AAGATTGGCT CGTTTGGGCC ACAAGAAGAT 
GTTCTGTTCC AGCAAGCTTC AGAAATGGCA AGAGAAAGTG GCATTCCACG GATCTATATT 
GCTGCCAACA GCGGAGCTCG TATAGGCCTG GCAGAGGAGA TCAGACACAT GTTTCATGTG 
GCCTGGCAGG ATCCGGTGGA CCCATACAAG GGTTTTAAGT ACCTATACCT CACACCACAG 
GACTATAAGA AGGTGTCTGC TCTGAACTCA GTCCACTGTG AACATGTGGA GGATGAGGGG 
GAATCCAGGT ATAAAATCAC TGATATCATT GGGAAAGAGG AAGGGTTGGG TGTGGAGAAC 
TTGAGAGGTT CTGGCATGAT CGCAGGTGAA TCGTCACTGG CGTATGAGGA TATTATCACC 
ATGAATCTGG TGACATGCAG GGCCATTGGA ATCGGAGCGT ACTTGGTGAG ACTGGGCCAA 
AGAACCATAC AGGTGGAAAA CTCTCATATC ATCCTGACTG GAGCCGGAGC TCTCAATAAG 
GTTCTCGGGC GTGAGGTGTA CACTTCCAAT AACCAACTGG GTGGAGTACA GATCATGCAC 
AATAACGGAG TCACACACAC CAATGTGTGC GATGATTTTG AGGGAGTGTA TACATTGCTG 
CACTGGCTGT CTTACATGCC AAAGAATATG TCCAGCCCTG TGCCGATACT CTCTGCTAAA 
GATCCCATTG ATCGAGCGAT AGAGTTTGTC CCAACCAAGG CACCTTATGA CCCACGCTGG 
ATGCTAGCTG GACGTCCCAA TCAGAACATT AAGGGTGCAT GGGTGAGTGG GTTCTTCGAC 
CAGGGCTCTT TTCTGGAGAT CATGCAGCCA TGGGCACAGA GTGTTGTCGT AGGACGGGCC 
AGGTTGGGTG GGATCCCAAC TGGAGTAGTT GCCGTGGAAA CCCGATCAGT GGAGCTTTCA 
ATCCCAGCAG ACCCAGCTAA TCTGGACTCG GAAGCCAAGA TAATCCAGCA GGCTGGGCAA 
GTGTGGTTCC CTGATTCTGC TTTCAAAACT GCACAAGCCA TTAAAGACTT CAATAGAGAG 
GGACTTCCGC TTATAGTGTT TGCCAACTGG AGAGGTTTTT CTGGAGGCAT GAAAGACATG 
TATGACCAGG TGCTGAAGTT TGGAGCGTAT ATAGTGGACG GTCTGAGAGA GTACAGACAG 
CCGGTTCTGG TGTATATCCC ACCACAGGCG GAGCTCCGGG GAGGATCATG GGTTGTCATA 
GACCCTACTA TCAATCCTCG CCACATGGAA ATGTATGCAG ACAAAGACAG CCGTGGCGGT 
GTGCTGGAGC CTGAAGGCAC AGTGGAGATC AAGTTCCGCA AAAAAGACCT GGTGAAGACC 
ATGCGACGGG TGGACCCTGT GTACATGGGG CTTGCAGAAA GACTGGGAAC CCCAGAGTTG 
AGTGTGTCTG AGCGTAAAGA GCTGGAGAGT AAACTAAAAG AGAGAGAAGA GTTCCTGCTG 
CCCATATATC ATCAAGTAGC TGTTCAGTTC GCTGACCTTC ATGACACACC GGGCCGCATG 
CAGGAGAAAG GAGTCATCAC TGATATCCTT GAGTGGAGCA CGTCTCGGCA GTTTTTCTAC 
TGGCGTTTGC GGAGGTTATT GCTGGAGGAA ACGGTGAAGA GGAAGATCCA GTGTGCTAAC 
AGTGAGCTGA CGGACGGACA GGTTCAGGCT ATGCTTCGCC GTTGGTTCGT GGAAGCAGAG 
GGAGCTGTGA AAGCGTATTT GTGGGACAGT AATGAAGATG TGGTGGAGTG GTTGGAGAGG 
CAGCTGGCAG AGGAAGAAGG CGCCAGATCC GTCATCGACG AGAATATCAA ATATATTCGC 
AGAGATCACA TCCTCAAACA GATCCGCAGC CTGGTTCAAG CTAACCCTGA GGTGGCTATG 
GACTCCATTG TACACATGAC CCAGCACATT TCCCCCACAC AGCGCGCCGA AGTCGTCCGC 
ATCCTCTCCA CCATGGACAC CCCAGCCTCC TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_021476197.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331872.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X7, mRNA.

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CloneID	ODa142654
Clone ID Related Accession (Same CDS sequence)	XM_021476197.1
Accession Version	XM_021476197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAATGTCCT TTTCCTCAAA CCTGAACCAC 
TACGGCATGG TCCACGTGGC CAGCGTTAGT GATGTGCTCC TAGACACCTC CTTTACCCCA 
CCCTGTCAGC GCATGGGAGC CATGGTTTCC TTCCGCTCTT TCCAGGAGTT CACCAGGAAC 
ATAAAGGATG TTCTGAGCTG TTTCTCAGAC TCTCCTCCAT CAACTCCAAC CTTTCCTGAA 
GGGGGGAACC CTGTGCTGTA TAGAGAGGAG GACAGCAAGA GCATTCAAGA TGAGCCCATT 
CATATTCTTA ATGTGGCAAT AAAGACGGAC AGTGATATTG ATGATGATGG GCTGGCGGCC 
ATGTTTCGTG AGTTTACACA GTCTAAGAAA TCTCTGCTGT TTGACCACGG CATCCGAAGA 
CTTACCTTCC TGGTTGCTCA GAAGGATTTC AGAAAGCAAA TCAACTGTGA GGTGGACCAG 
AGGTTCCATA GAGAATTCCC CAAATTTTTT ACATTCCGGG CTCGAGACAA GTTTGAGGAA 
GACCGTATCT ACAGACACTT GGAACCTGCA TTAGCTTTCC AGCTGGAGCT CAACCGCATG 
CGTAATTTTG CACTGACAGC CATCCCCTGT GCCAATCATA AGATGCACCT GTACTTGGGA 
GCTGCCCGTG TGGAAGTAGG CACTGAGGTC ACAGATTACC GCTTCTTTGT GCGGGCAATC 
ATCCGTCACT CAGATTTGGT CACCAAGGAA GCATCATTTG AGTACCTGCA CAACGAGGCA 
GAACGCTTGC TGCTGGAGGC CATGGATGAA CTGGAAGTGG CTTTCAACAA CACCACTGTG 
CGAACCGACT GCAATCACAT CTTCCTCAAC TTCGTTCCAA CAGTCATCAT GGACCCTTCA 
AAGCATTCAG CAATCCCATC AATACAGACA ATTTTGAAAA CAACACTGGC CGAAATGCAG 
CCCCAAACCA GGACAAACCA GAGACTCCAA CATGTTTCAC TGAAGGCTAT TGAGGAATCT 
GTGCGCTCCA TGGTCATGCG CTACGGCAGT CGCTTGTGGA AGCTCCGTGT GCTTCAAGCC 
GAGCTGAAGA TCAACATCCG ATTGACTCCC ACCGGCAAAC AGATCCCCAT CCGCCTCTTC 
CTGACCAACG AGAGTGGCTA CTACCTGGAC ATCAGCCTGT ACAAGGAAGT CACTGACTCC 
CGTACAGGAC AGGTGGGGCA CAAAGACCGA CAGATCATGT TCCAGGCATA TGGAGATAAG 
CAGGGTCCTC TTCATGGCAT GCTCATCAAC ACACCCTACG TCACAAAAGA CCTGCTGCAG 
TCCAAACGCT TCCAGGCACA GAGCCTCGGC ACTACCTATG TTTACGACTT CCCAGAAATG 
TTTCGCCAGG CTTTAAGGAA GCTGTGGCAA TCCATGGATG CACACGCCAA CTTGCCCAAG 
TGCCCTCTTC CATCAGAACT GCTCACCTTC ACAGAGCTGG TTCTGGATTC TCAGGGTCAG 
CTGGTGCAGA TGAACCGCCT ACCTGGAGGG AATGAGATTG GCATGGTAGC ATGGCGAATG 
ACCCTCCGGA CCCCTGAGTA TCCAGCCGGC CGTGAGATTA TAGTGATCAG CAATGACATC 
ACACATAAGA TTGGCTCGTT TGGGCCACAA GAAGATGTTC TGTTCCAGCA AGCTTCAGAA 
ATGGCAAGAG AAAGTGGCAT TCCACGGATC TATATTGCTG CCAACAGCGG AGCTCGTATA 
GGCCTGGCAG AGGAGATCAG ACACATGTTT CATGTGGCCT GGCAGGATCC GGTGGACCCA 
TACAAGGGTT TTAAGTACCT ATACCTCACA CCACAGGACT ATAAGAAGGT GTCTGCTCTG 
AACTCAGTCC ACTGTGAACA TGTGGAGGAT GAGGGGGAAT CCAGGTATAA AATCACTGAT 
ATCATTGGGA AAGAGGAAGG GTTGGGTGTG GAGAACTTGA GAGGTTCTGG CATGATCGCA 
GGTGAATCGT CACTGGCGTA TGAGGATATT ATCACCATGA ATCTGGTGAC ATGCAGGGCC 
ATTGGAATCG GAGCGTACTT GGTGAGACTG GGCCAAAGAA CCATACAGGT GGAAAACTCT 
CATATCATCC TGACTGGAGC CGGAGCTCTC AATAAGGTTC TCGGGCGTGA GGTGTACACT 
TCCAATAACC AACTGGGTGG AGTACAGATC ATGCACAATA ACGGAGTCAC ACACACCAAT 
GTGTGCGATG ATTTTGAGGG AGTGTATACA TTGCTGCACT GGCTGTCTTA CATGCCAAAG 
AATATGTCCA GCCCTGTGCC GATACTCTCT GCTAAAGATC CCATTGATCG AGCGATAGAG 
TTTGTCCCAA CCAAGGCACC TTATGACCCA CGCTGGATGC TAGCTGGACG TCCCAATCAG 
AACATTAAGG GTGCATGGGT GAGTGGGTTC TTCGACCAGG GCTCTTTTCT GGAGATCATG 
CAGCCATGGG CACAGAGTGT TGTCGTAGGA CGGGCCAGGT TGGGTGGGAT CCCAACTGGA 
GTAGTTGCCG TGGAAACCCG ATCAGTGGAG CTTTCAATCC CAGCAGACCC AGCTAATCTG 
GACTCGGAAG CCAAGATAAT CCAGCAGGCT GGGCAAGTGT GGTTCCCTGA TTCTGCTTTC 
AAAACTGCAC AAGCCATTAA AGACTTCAAT AGAGAGGGAC TTCCGCTTAT AGTGTTTGCC 
AACTGGAGAG GTTTTTCTGG AGGCATGAAA GACATGTATG ACCAGGTGCT GAAGTTTGGA 
GCGTATATAG TGGACGGTCT GAGAGAGTAC AGACAGCCGG TTCTGGTGTA TATCCCACCA 
CAGGCGGAGC TCCGGGGAGG ATCATGGGTT GTCATAGACC CTACTATCAA TCCTCGCCAC 
ATGGAAATGT ATGCAGACAA AGACAGCCGT GGCGGTGTGC TGGAGCCTGA AGGCACAGTG 
GAGATCAAGT TCCGCAAAAA AGACCTGGTG AAGACCATGC GACGGGTGGA CCCTGTGTAC 
ATGGGGCTTG CAGAAAGACT GGGAACCCCA GAGTTGAGTG TGTCTGAGCG TAAAGAGCTG 
GAGAGTAAAC TAAAAGAGAG AGAAGAGTTC CTGCTGCCCA TATATCATCA AGTAGCTGTT 
CAGTTCGCTG ACCTTCATGA CACACCGGGC CGCATGCAGG AGAAAGGAGT CATCACTGAT 
ATCCTTGAGT GGAGCACGTC TCGGCAGTTT TTCTACTGGC GTTTGCGGAG GTTATTGCTG 
GAGGAAACGG TGAAGAGGAA GATCCAGTGT GCTAACAGTG AGCTGACGGA CGGACAGGTT 
CAGGCTATGC TTCGCCGTTG GTTCGTGGAA GCAGAGGGAG CTGTGAAAGC GTATTTGTGG 
GACAGTAATG AAGATGTGGT GGAGTGGTTG GAGAGGCAGC TGGCAGAGGA AGAAGGCGCC 
AGATCCGTCA TCGACGAGAA TATCAAATAT ATTCGCAGAG ATCACATCCT CAAACAGATC 
CGCAGCCTGG TTCAAGCTAA CCCTGAGGTG GCTATGGACT CCATTGTACA CATGACCCAG 
CACATTTCCC CCACACAGCG CGCCGAAGTC GTCCGCATCC TCTCCACCAT GGACACCCCA 
GCCTCCTCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331872.1
CDS	237..7388
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKHSAIPSIQTILKTTLAEMQPQTRTNQRLQHVSLKAIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_021476197.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476197.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAATGTCCT TTTCCTCAAA CCTGAACCAC 
TACGGCATGG TCCACGTGGC CAGCGTTAGT GATGTGCTCC TAGACACCTC CTTTACCCCA 
CCCTGTCAGC GCATGGGAGC CATGGTTTCC TTCCGCTCTT TCCAGGAGTT CACCAGGAAC 
ATAAAGGATG TTCTGAGCTG TTTCTCAGAC TCTCCTCCAT CAACTCCAAC CTTTCCTGAA 
GGGGGGAACC CTGTGCTGTA TAGAGAGGAG GACAGCAAGA GCATTCAAGA TGAGCCCATT 
CATATTCTTA ATGTGGCAAT AAAGACGGAC AGTGATATTG ATGATGATGG GCTGGCGGCC 
ATGTTTCGTG AGTTTACACA GTCTAAGAAA TCTCTGCTGT TTGACCACGG CATCCGAAGA 
CTTACCTTCC TGGTTGCTCA GAAGGATTTC AGAAAGCAAA TCAACTGTGA GGTGGACCAG 
AGGTTCCATA GAGAATTCCC CAAATTTTTT ACATTCCGGG CTCGAGACAA GTTTGAGGAA 
GACCGTATCT ACAGACACTT GGAACCTGCA TTAGCTTTCC AGCTGGAGCT CAACCGCATG 
CGTAATTTTG CACTGACAGC CATCCCCTGT GCCAATCATA AGATGCACCT GTACTTGGGA 
GCTGCCCGTG TGGAAGTAGG CACTGAGGTC ACAGATTACC GCTTCTTTGT GCGGGCAATC 
ATCCGTCACT CAGATTTGGT CACCAAGGAA GCATCATTTG AGTACCTGCA CAACGAGGCA 
GAACGCTTGC TGCTGGAGGC CATGGATGAA CTGGAAGTGG CTTTCAACAA CACCACTGTG 
CGAACCGACT GCAATCACAT CTTCCTCAAC TTCGTTCCAA CAGTCATCAT GGACCCTTCA 
AAGCATTCAG CAATCCCATC AATACAGACA ATTTTGAAAA CAACACTGGC CGAAATGCAG 
CCCCAAACCA GGACAAACCA GAGACTCCAA CATGTTTCAC TGAAGGCTAT TGAGGAATCT 
GTGCGCTCCA TGGTCATGCG CTACGGCAGT CGCTTGTGGA AGCTCCGTGT GCTTCAAGCC 
GAGCTGAAGA TCAACATCCG ATTGACTCCC ACCGGCAAAC AGATCCCCAT CCGCCTCTTC 
CTGACCAACG AGAGTGGCTA CTACCTGGAC ATCAGCCTGT ACAAGGAAGT CACTGACTCC 
CGTACAGGAC AGGTGGGGCA CAAAGACCGA CAGATCATGT TCCAGGCATA TGGAGATAAG 
CAGGGTCCTC TTCATGGCAT GCTCATCAAC ACACCCTACG TCACAAAAGA CCTGCTGCAG 
TCCAAACGCT TCCAGGCACA GAGCCTCGGC ACTACCTATG TTTACGACTT CCCAGAAATG 
TTTCGCCAGG CTTTAAGGAA GCTGTGGCAA TCCATGGATG CACACGCCAA CTTGCCCAAG 
TGCCCTCTTC CATCAGAACT GCTCACCTTC ACAGAGCTGG TTCTGGATTC TCAGGGTCAG 
CTGGTGCAGA TGAACCGCCT ACCTGGAGGG AATGAGATTG GCATGGTAGC ATGGCGAATG 
ACCCTCCGGA CCCCTGAGTA TCCAGCCGGC CGTGAGATTA TAGTGATCAG CAATGACATC 
ACACATAAGA TTGGCTCGTT TGGGCCACAA GAAGATGTTC TGTTCCAGCA AGCTTCAGAA 
ATGGCAAGAG AAAGTGGCAT TCCACGGATC TATATTGCTG CCAACAGCGG AGCTCGTATA 
GGCCTGGCAG AGGAGATCAG ACACATGTTT CATGTGGCCT GGCAGGATCC GGTGGACCCA 
TACAAGGGTT TTAAGTACCT ATACCTCACA CCACAGGACT ATAAGAAGGT GTCTGCTCTG 
AACTCAGTCC ACTGTGAACA TGTGGAGGAT GAGGGGGAAT CCAGGTATAA AATCACTGAT 
ATCATTGGGA AAGAGGAAGG GTTGGGTGTG GAGAACTTGA GAGGTTCTGG CATGATCGCA 
GGTGAATCGT CACTGGCGTA TGAGGATATT ATCACCATGA ATCTGGTGAC ATGCAGGGCC 
ATTGGAATCG GAGCGTACTT GGTGAGACTG GGCCAAAGAA CCATACAGGT GGAAAACTCT 
CATATCATCC TGACTGGAGC CGGAGCTCTC AATAAGGTTC TCGGGCGTGA GGTGTACACT 
TCCAATAACC AACTGGGTGG AGTACAGATC ATGCACAATA ACGGAGTCAC ACACACCAAT 
GTGTGCGATG ATTTTGAGGG AGTGTATACA TTGCTGCACT GGCTGTCTTA CATGCCAAAG 
AATATGTCCA GCCCTGTGCC GATACTCTCT GCTAAAGATC CCATTGATCG AGCGATAGAG 
TTTGTCCCAA CCAAGGCACC TTATGACCCA CGCTGGATGC TAGCTGGACG TCCCAATCAG 
AACATTAAGG GTGCATGGGT GAGTGGGTTC TTCGACCAGG GCTCTTTTCT GGAGATCATG 
CAGCCATGGG CACAGAGTGT TGTCGTAGGA CGGGCCAGGT TGGGTGGGAT CCCAACTGGA 
GTAGTTGCCG TGGAAACCCG ATCAGTGGAG CTTTCAATCC CAGCAGACCC AGCTAATCTG 
GACTCGGAAG CCAAGATAAT CCAGCAGGCT GGGCAAGTGT GGTTCCCTGA TTCTGCTTTC 
AAAACTGCAC AAGCCATTAA AGACTTCAAT AGAGAGGGAC TTCCGCTTAT AGTGTTTGCC 
AACTGGAGAG GTTTTTCTGG AGGCATGAAA GACATGTATG ACCAGGTGCT GAAGTTTGGA 
GCGTATATAG TGGACGGTCT GAGAGAGTAC AGACAGCCGG TTCTGGTGTA TATCCCACCA 
CAGGCGGAGC TCCGGGGAGG ATCATGGGTT GTCATAGACC CTACTATCAA TCCTCGCCAC 
ATGGAAATGT ATGCAGACAA AGACAGCCGT GGCGGTGTGC TGGAGCCTGA AGGCACAGTG 
GAGATCAAGT TCCGCAAAAA AGACCTGGTG AAGACCATGC GACGGGTGGA CCCTGTGTAC 
ATGGGGCTTG CAGAAAGACT GGGAACCCCA GAGTTGAGTG TGTCTGAGCG TAAAGAGCTG 
GAGAGTAAAC TAAAAGAGAG AGAAGAGTTC CTGCTGCCCA TATATCATCA AGTAGCTGTT 
CAGTTCGCTG ACCTTCATGA CACACCGGGC CGCATGCAGG AGAAAGGAGT CATCACTGAT 
ATCCTTGAGT GGAGCACGTC TCGGCAGTTT TTCTACTGGC GTTTGCGGAG GTTATTGCTG 
GAGGAAACGG TGAAGAGGAA GATCCAGTGT GCTAACAGTG AGCTGACGGA CGGACAGGTT 
CAGGCTATGC TTCGCCGTTG GTTCGTGGAA GCAGAGGGAG CTGTGAAAGC GTATTTGTGG 
GACAGTAATG AAGATGTGGT GGAGTGGTTG GAGAGGCAGC TGGCAGAGGA AGAAGGCGCC 
AGATCCGTCA TCGACGAGAA TATCAAATAT ATTCGCAGAG ATCACATCCT CAAACAGATC 
CGCAGCCTGG TTCAAGCTAA CCCTGAGGTG GCTATGGACT CCATTGTACA CATGACCCAG 
CACATTTCCC CCACACAGCG CGCCGAAGTC GTCCGCATCC TCTCCACCAT GGACACCCCA 
GCCTCCTCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_021476198.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331873.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X9, mRNA.

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CloneID	ODa142655
Clone ID Related Accession (Same CDS sequence)	XM_021476198.1
Accession Version	XM_021476198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGAATG 
TCCTTTTCCT CAAACCTGAA CCACTACGGC ATGGTCCACG TGGCCAGCGT TAGTGATGTG 
CTCCTAGACA CCTCCTTTAC CCCACCCTGT CAGCGCATGG GAGCCATGGT TTCCTTCCGC 
TCTTTCCAGG AGTTCACCAG GAACATAAAG GATGTTCTGA GCTGTTTCTC AGACTCTCCT 
CCATCAACTC CAACCTTTCC TGAAGGGGGG AACCCTGTGC TGTATAGAGA GGAGGACAGC 
AAGAGCATTC AAGATGAGCC CATTCATATT CTTAATGTGG CAATAAAGAC GGACAGTGAT 
ATTGATGATG ATGGGCTGGC GGCCATGTTT CGTGAGTTTA CACAGTCTAA GAAATCTCTG 
CTGTTTGACC ACGGCATCCG AAGACTTACC TTCCTGGTTG CTCAGAAGAG AGAATTCCCC 
AAATTTTTTA CATTCCGGGC TCGAGACAAG TTTGAGGAAG ACCGTATCTA CAGACACTTG 
GAACCTGCAT TAGCTTTCCA GCTGGAGCTC AACCGCATGC GTAATTTTGC ACTGACAGCC 
ATCCCCTGTG CCAATCATAA GATGCACCTG TACTTGGGAG CTGCCCGTGT GGAAGTAGGC 
ACTGAGGTCA CAGATTACCG CTTCTTTGTG CGGGCAATCA TCCGTCACTC AGATTTGGTC 
ACCAAGGAAG CATCATTTGA GTACCTGCAC AACGAGGCAG AACGCTTGCT GCTGGAGGCC 
ATGGATGAAC TGGAAGTGGC TTTCAACAAC ACCACTGTGC GAACCGACTG CAATCACATC 
TTCCTCAACT TCGTTCCAAC AGTCATCATG GACCCTTCAA AGCATTCAGC AATCCCATCA 
ATACAGACAA TTTTGAAAAC AACACTGGCC GAAATGCAGC CCCAAACCAG GACAAACCAG 
AGACTCCAAC ATGTTTCACT GAAGGCTATT GAGGAATCTG TGCGCTCCAT GGTCATGCGC 
TACGGCAGTC GCTTGTGGAA GCTCCGTGTG CTTCAAGCCG AGCTGAAGAT CAACATCCGA 
TTGACTCCCA CCGGCAAACA GATCCCCATC CGCCTCTTCC TGACCAACGA GAGTGGCTAC 
TACCTGGACA TCAGCCTGTA CAAGGAAGTC ACTGACTCCC GTACAGGACA GGTGGGGCAC 
AAAGACCGAC AGATCATGTT CCAGGCATAT GGAGATAAGC AGGGTCCTCT TCATGGCATG 
CTCATCAACA CACCCTACGT CACAAAAGAC CTGCTGCAGT CCAAACGCTT CCAGGCACAG 
AGCCTCGGCA CTACCTATGT TTACGACTTC CCAGAAATGT TTCGCCAGGC TTTAAGGAAG 
CTGTGGCAAT CCATGGATGC ACACGCCAAC TTGCCCAAGT GCCCTCTTCC ATCAGAACTG 
CTCACCTTCA CAGAGCTGGT TCTGGATTCT CAGGGTCAGC TGGTGCAGAT GAACCGCCTA 
CCTGGAGGGA ATGAGATTGG CATGGTAGCA TGGCGAATGA CCCTCCGGAC CCCTGAGTAT 
CCAGCCGGCC GTGAGATTAT AGTGATCAGC AATGACATCA CACATAAGAT TGGCTCGTTT 
GGGCCACAAG AAGATGTTCT GTTCCAGCAA GCTTCAGAAA TGGCAAGAGA AAGTGGCATT 
CCACGGATCT ATATTGCTGC CAACAGCGGA GCTCGTATAG GCCTGGCAGA GGAGATCAGA 
CACATGTTTC ATGTGGCCTG GCAGGATCCG GTGGACCCAT ACAAGGGTTT TAAGTACCTA 
TACCTCACAC CACAGGACTA TAAGAAGGTG TCTGCTCTGA ACTCAGTCCA CTGTGAACAT 
GTGGAGGATG AGGGGGAATC CAGGTATAAA ATCACTGATA TCATTGGGAA AGAGGAAGGG 
TTGGGTGTGG AGAACTTGAG AGGTTCTGGC ATGATCGCAG GTGAATCGTC ACTGGCGTAT 
GAGGATATTA TCACCATGAA TCTGGTGACA TGCAGGGCCA TTGGAATCGG AGCGTACTTG 
GTGAGACTGG GCCAAAGAAC CATACAGGTG GAAAACTCTC ATATCATCCT GACTGGAGCC 
GGAGCTCTCA ATAAGGTTCT CGGGCGTGAG GTGTACACTT CCAATAACCA ACTGGGTGGA 
GTACAGATCA TGCACAATAA CGGAGTCACA CACACCAATG TGTGCGATGA TTTTGAGGGA 
GTGTATACAT TGCTGCACTG GCTGTCTTAC ATGCCAAAGA ATATGTCCAG CCCTGTGCCG 
ATACTCTCTG CTAAAGATCC CATTGATCGA GCGATAGAGT TTGTCCCAAC CAAGGCACCT 
TATGACCCAC GCTGGATGCT AGCTGGACGT CCCAATCAGA ACATTAAGGG TGCATGGGTG 
AGTGGGTTCT TCGACCAGGG CTCTTTTCTG GAGATCATGC AGCCATGGGC ACAGAGTGTT 
GTCGTAGGAC GGGCCAGGTT GGGTGGGATC CCAACTGGAG TAGTTGCCGT GGAAACCCGA 
TCAGTGGAGC TTTCAATCCC AGCAGACCCA GCTAATCTGG ACTCGGAAGC CAAGATAATC 
CAGCAGGCTG GGCAAGTGTG GTTCCCTGAT TCTGCTTTCA AAACTGCACA AGCCATTAAA 
GACTTCAATA GAGAGGGACT TCCGCTTATA GTGTTTGCCA ACTGGAGAGG TTTTTCTGGA 
GGCATGAAAG ACATGTATGA CCAGGTGCTG AAGTTTGGAG CGTATATAGT GGACGGTCTG 
AGAGAGTACA GACAGCCGGT TCTGGTGTAT ATCCCACCAC AGGCGGAGCT CCGGGGAGGA 
TCATGGGTTG TCATAGACCC TACTATCAAT CCTCGCCACA TGGAAATGTA TGCAGACAAA 
GACAGCCGTG GCGGTGTGCT GGAGCCTGAA GGCACAGTGG AGATCAAGTT CCGCAAAAAA 
GACCTGGTGA AGACCATGCG ACGGGTGGAC CCTGTGTACA TGGGGCTTGC AGAAAGACTG 
GGAACCCCAG AGTTGAGTGT GTCTGAGCGT AAAGAGCTGG AGAGTAAACT AAAAGAGAGA 
GAAGAGTTCC TGCTGCCCAT ATATCATCAA GTAGCTGTTC AGTTCGCTGA CCTTCATGAC 
ACACCGGGCC GCATGCAGGA GAAAGGAGTC ATCACTGATA TCCTTGAGTG GAGCACGTCT 
CGGCAGTTTT TCTACTGGCG TTTGCGGAGG TTATTGCTGG AGGAAACGGT GAAGAGGAAG 
ATCCAGTGTG CTAACAGTGA GCTGACGGAC GGACAGGTTC AGGCTATGCT TCGCCGTTGG 
TTCGTGGAAG CAGAGGGAGC TGTGAAAGCG TATTTGTGGG ACAGTAATGA AGATGTGGTG 
GAGTGGTTGG AGAGGCAGCT GGCAGAGGAA GAAGGCGCCA GATCCGTCAT CGACGAGAAT 
ATCAAATATA TTCGCAGAGA TCACATCCTC AAACAGATCC GCAGCCTGGT TCAAGCTAAC 
CCTGAGGTGG CTATGGACTC CATTGTACAC ATGACCCAGC ACATTTCCCC CACACAGCGC 
GCCGAAGTCG TCCGCATCCT CTCCACCATG GACACCCCAG CCTCCTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331873.1
CDS	237..7367
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKHSAIPSIQTILKTTLAEMQPQTRTNQRLQHVSLKAIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_021476198.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476198.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGAATG 
TCCTTTTCCT CAAACCTGAA CCACTACGGC ATGGTCCACG TGGCCAGCGT TAGTGATGTG 
CTCCTAGACA CCTCCTTTAC CCCACCCTGT CAGCGCATGG GAGCCATGGT TTCCTTCCGC 
TCTTTCCAGG AGTTCACCAG GAACATAAAG GATGTTCTGA GCTGTTTCTC AGACTCTCCT 
CCATCAACTC CAACCTTTCC TGAAGGGGGG AACCCTGTGC TGTATAGAGA GGAGGACAGC 
AAGAGCATTC AAGATGAGCC CATTCATATT CTTAATGTGG CAATAAAGAC GGACAGTGAT 
ATTGATGATG ATGGGCTGGC GGCCATGTTT CGTGAGTTTA CACAGTCTAA GAAATCTCTG 
CTGTTTGACC ACGGCATCCG AAGACTTACC TTCCTGGTTG CTCAGAAGAG AGAATTCCCC 
AAATTTTTTA CATTCCGGGC TCGAGACAAG TTTGAGGAAG ACCGTATCTA CAGACACTTG 
GAACCTGCAT TAGCTTTCCA GCTGGAGCTC AACCGCATGC GTAATTTTGC ACTGACAGCC 
ATCCCCTGTG CCAATCATAA GATGCACCTG TACTTGGGAG CTGCCCGTGT GGAAGTAGGC 
ACTGAGGTCA CAGATTACCG CTTCTTTGTG CGGGCAATCA TCCGTCACTC AGATTTGGTC 
ACCAAGGAAG CATCATTTGA GTACCTGCAC AACGAGGCAG AACGCTTGCT GCTGGAGGCC 
ATGGATGAAC TGGAAGTGGC TTTCAACAAC ACCACTGTGC GAACCGACTG CAATCACATC 
TTCCTCAACT TCGTTCCAAC AGTCATCATG GACCCTTCAA AGCATTCAGC AATCCCATCA 
ATACAGACAA TTTTGAAAAC AACACTGGCC GAAATGCAGC CCCAAACCAG GACAAACCAG 
AGACTCCAAC ATGTTTCACT GAAGGCTATT GAGGAATCTG TGCGCTCCAT GGTCATGCGC 
TACGGCAGTC GCTTGTGGAA GCTCCGTGTG CTTCAAGCCG AGCTGAAGAT CAACATCCGA 
TTGACTCCCA CCGGCAAACA GATCCCCATC CGCCTCTTCC TGACCAACGA GAGTGGCTAC 
TACCTGGACA TCAGCCTGTA CAAGGAAGTC ACTGACTCCC GTACAGGACA GGTGGGGCAC 
AAAGACCGAC AGATCATGTT CCAGGCATAT GGAGATAAGC AGGGTCCTCT TCATGGCATG 
CTCATCAACA CACCCTACGT CACAAAAGAC CTGCTGCAGT CCAAACGCTT CCAGGCACAG 
AGCCTCGGCA CTACCTATGT TTACGACTTC CCAGAAATGT TTCGCCAGGC TTTAAGGAAG 
CTGTGGCAAT CCATGGATGC ACACGCCAAC TTGCCCAAGT GCCCTCTTCC ATCAGAACTG 
CTCACCTTCA CAGAGCTGGT TCTGGATTCT CAGGGTCAGC TGGTGCAGAT GAACCGCCTA 
CCTGGAGGGA ATGAGATTGG CATGGTAGCA TGGCGAATGA CCCTCCGGAC CCCTGAGTAT 
CCAGCCGGCC GTGAGATTAT AGTGATCAGC AATGACATCA CACATAAGAT TGGCTCGTTT 
GGGCCACAAG AAGATGTTCT GTTCCAGCAA GCTTCAGAAA TGGCAAGAGA AAGTGGCATT 
CCACGGATCT ATATTGCTGC CAACAGCGGA GCTCGTATAG GCCTGGCAGA GGAGATCAGA 
CACATGTTTC ATGTGGCCTG GCAGGATCCG GTGGACCCAT ACAAGGGTTT TAAGTACCTA 
TACCTCACAC CACAGGACTA TAAGAAGGTG TCTGCTCTGA ACTCAGTCCA CTGTGAACAT 
GTGGAGGATG AGGGGGAATC CAGGTATAAA ATCACTGATA TCATTGGGAA AGAGGAAGGG 
TTGGGTGTGG AGAACTTGAG AGGTTCTGGC ATGATCGCAG GTGAATCGTC ACTGGCGTAT 
GAGGATATTA TCACCATGAA TCTGGTGACA TGCAGGGCCA TTGGAATCGG AGCGTACTTG 
GTGAGACTGG GCCAAAGAAC CATACAGGTG GAAAACTCTC ATATCATCCT GACTGGAGCC 
GGAGCTCTCA ATAAGGTTCT CGGGCGTGAG GTGTACACTT CCAATAACCA ACTGGGTGGA 
GTACAGATCA TGCACAATAA CGGAGTCACA CACACCAATG TGTGCGATGA TTTTGAGGGA 
GTGTATACAT TGCTGCACTG GCTGTCTTAC ATGCCAAAGA ATATGTCCAG CCCTGTGCCG 
ATACTCTCTG CTAAAGATCC CATTGATCGA GCGATAGAGT TTGTCCCAAC CAAGGCACCT 
TATGACCCAC GCTGGATGCT AGCTGGACGT CCCAATCAGA ACATTAAGGG TGCATGGGTG 
AGTGGGTTCT TCGACCAGGG CTCTTTTCTG GAGATCATGC AGCCATGGGC ACAGAGTGTT 
GTCGTAGGAC GGGCCAGGTT GGGTGGGATC CCAACTGGAG TAGTTGCCGT GGAAACCCGA 
TCAGTGGAGC TTTCAATCCC AGCAGACCCA GCTAATCTGG ACTCGGAAGC CAAGATAATC 
CAGCAGGCTG GGCAAGTGTG GTTCCCTGAT TCTGCTTTCA AAACTGCACA AGCCATTAAA 
GACTTCAATA GAGAGGGACT TCCGCTTATA GTGTTTGCCA ACTGGAGAGG TTTTTCTGGA 
GGCATGAAAG ACATGTATGA CCAGGTGCTG AAGTTTGGAG CGTATATAGT GGACGGTCTG 
AGAGAGTACA GACAGCCGGT TCTGGTGTAT ATCCCACCAC AGGCGGAGCT CCGGGGAGGA 
TCATGGGTTG TCATAGACCC TACTATCAAT CCTCGCCACA TGGAAATGTA TGCAGACAAA 
GACAGCCGTG GCGGTGTGCT GGAGCCTGAA GGCACAGTGG AGATCAAGTT CCGCAAAAAA 
GACCTGGTGA AGACCATGCG ACGGGTGGAC CCTGTGTACA TGGGGCTTGC AGAAAGACTG 
GGAACCCCAG AGTTGAGTGT GTCTGAGCGT AAAGAGCTGG AGAGTAAACT AAAAGAGAGA 
GAAGAGTTCC TGCTGCCCAT ATATCATCAA GTAGCTGTTC AGTTCGCTGA CCTTCATGAC 
ACACCGGGCC GCATGCAGGA GAAAGGAGTC ATCACTGATA TCCTTGAGTG GAGCACGTCT 
CGGCAGTTTT TCTACTGGCG TTTGCGGAGG TTATTGCTGG AGGAAACGGT GAAGAGGAAG 
ATCCAGTGTG CTAACAGTGA GCTGACGGAC GGACAGGTTC AGGCTATGCT TCGCCGTTGG 
TTCGTGGAAG CAGAGGGAGC TGTGAAAGCG TATTTGTGGG ACAGTAATGA AGATGTGGTG 
GAGTGGTTGG AGAGGCAGCT GGCAGAGGAA GAAGGCGCCA GATCCGTCAT CGACGAGAAT 
ATCAAATATA TTCGCAGAGA TCACATCCTC AAACAGATCC GCAGCCTGGT TCAAGCTAAC 
CCTGAGGTGG CTATGGACTC CATTGTACAC ATGACCCAGC ACATTTCCCC CACACAGCGC 
GCCGAAGTCG TCCGCATCCT CTCCACCATG GACACCCCAG CCTCCTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_021476199.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331874.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X13, mRNA.

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CloneID	ODa142656
Clone ID Related Accession (Same CDS sequence)	XM_021476199.1
Accession Version	XM_021476199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAATGTCCT TTTCCTCAAA CCTGAACCAC 
TACGGCATGG TCCACGTGGC CAGCGTTAGT GATGTGCTCC TAGACACCTC CTTTACCCCA 
CCCTGTCAGC GCATGGGAGC CATGGTTTCC TTCCGCTCTT TCCAGGAGTT CACCAGGAAC 
ATAAAGGATG TTCTGAGCTG TTTCTCAGAC TCTCCTCCAT CAACTCCAAC CTTTCCTGAA 
GGGGGGAACC CTGTGCTGTA TAGAGAGGAG GACAGCAAGA GCATTCAAGA TGAGCCCATT 
CATATTCTTA ATGTGGCAAT AAAGACGGAC AGTGATATTG ATGATGATGG GCTGGCGGCC 
ATGTTTCGTG AGTTTACACA GTCTAAGAAA TCTCTGCTGT TTGACCACGG CATCCGAAGA 
CTTACCTTCC TGGTTGCTCA GAAGGATTTC AGAAAGCAAA TCAACTGTGA GGTGGACCAG 
AGGTTCCATA GAGAATTCCC CAAATTTTTT ACATTCCGGG CTCGAGACAA GTTTGAGGAA 
GACCGTATCT ACAGACACTT GGAACCTGCA TTAGCTTTCC AGCTGGAGCT CAACCGCATG 
CGTAATTTTG CACTGACAGC CATCCCCTGT GCCAATCATA AGATGCACCT GTACTTGGGA 
GCTGCCCGTG TGGAAGTAGG CACTGAGGTC ACAGATTACC GCTTCTTTGT GCGGGCAATC 
ATCCGTCACT CAGATTTGGT CACCAAGGAA GCATCATTTG AGTACCTGCA CAACGAGGCA 
GAACGCTTGC TGCTGGAGGC CATGGATGAA CTGGAAGTGG CTTTCAACAA CACCACTGTG 
CGAACCGACT GCAATCACAT CTTCCTCAAC TTCGTTCCAA CAGTCATCAT GGACCCTTCA 
AAGATTGAGG AATCTGTGCG CTCCATGGTC ATGCGCTACG GCAGTCGCTT GTGGAAGCTC 
CGTGTGCTTC AAGCCGAGCT GAAGATCAAC ATCCGATTGA CTCCCACCGG CAAACAGATC 
CCCATCCGCC TCTTCCTGAC CAACGAGAGT GGCTACTACC TGGACATCAG CCTGTACAAG 
GAAGTCACTG ACTCCCGTAC AGGACAGGTG GGGCACAAAG ACCGACAGAT CATGTTCCAG 
GCATATGGAG ATAAGCAGGG TCCTCTTCAT GGCATGCTCA TCAACACACC CTACGTCACA 
AAAGACCTGC TGCAGTCCAA ACGCTTCCAG GCACAGAGCC TCGGCACTAC CTATGTTTAC 
GACTTCCCAG AAATGTTTCG CCAGGCTTTA AGGAAGCTGT GGCAATCCAT GGATGCACAC 
GCCAACTTGC CCAAGTGCCC TCTTCCATCA GAACTGCTCA CCTTCACAGA GCTGGTTCTG 
GATTCTCAGG GTCAGCTGGT GCAGATGAAC CGCCTACCTG GAGGGAATGA GATTGGCATG 
GTAGCATGGC GAATGACCCT CCGGACCCCT GAGTATCCAG CCGGCCGTGA GATTATAGTG 
ATCAGCAATG ACATCACACA TAAGATTGGC TCGTTTGGGC CACAAGAAGA TGTTCTGTTC 
CAGCAAGCTT CAGAAATGGC AAGAGAAAGT GGCATTCCAC GGATCTATAT TGCTGCCAAC 
AGCGGAGCTC GTATAGGCCT GGCAGAGGAG ATCAGACACA TGTTTCATGT GGCCTGGCAG 
GATCCGGTGG ACCCATACAA GGGTTTTAAG TACCTATACC TCACACCACA GGACTATAAG 
AAGGTGTCTG CTCTGAACTC AGTCCACTGT GAACATGTGG AGGATGAGGG GGAATCCAGG 
TATAAAATCA CTGATATCAT TGGGAAAGAG GAAGGGTTGG GTGTGGAGAA CTTGAGAGGT 
TCTGGCATGA TCGCAGGTGA ATCGTCACTG GCGTATGAGG ATATTATCAC CATGAATCTG 
GTGACATGCA GGGCCATTGG AATCGGAGCG TACTTGGTGA GACTGGGCCA AAGAACCATA 
CAGGTGGAAA ACTCTCATAT CATCCTGACT GGAGCCGGAG CTCTCAATAA GGTTCTCGGG 
CGTGAGGTGT ACACTTCCAA TAACCAACTG GGTGGAGTAC AGATCATGCA CAATAACGGA 
GTCACACACA CCAATGTGTG CGATGATTTT GAGGGAGTGT ATACATTGCT GCACTGGCTG 
TCTTACATGC CAAAGAATAT GTCCAGCCCT GTGCCGATAC TCTCTGCTAA AGATCCCATT 
GATCGAGCGA TAGAGTTTGT CCCAACCAAG GCACCTTATG ACCCACGCTG GATGCTAGCT 
GGACGTCCCA ATCAGAACAT TAAGGGTGCA TGGGTGAGTG GGTTCTTCGA CCAGGGCTCT 
TTTCTGGAGA TCATGCAGCC ATGGGCACAG AGTGTTGTCG TAGGACGGGC CAGGTTGGGT 
GGGATCCCAA CTGGAGTAGT TGCCGTGGAA ACCCGATCAG TGGAGCTTTC AATCCCAGCA 
GACCCAGCTA ATCTGGACTC GGAAGCCAAG ATAATCCAGC AGGCTGGGCA AGTGTGGTTC 
CCTGATTCTG CTTTCAAAAC TGCACAAGCC ATTAAAGACT TCAATAGAGA GGGACTTCCG 
CTTATAGTGT TTGCCAACTG GAGAGGTTTT TCTGGAGGCA TGAAAGACAT GTATGACCAG 
GTGCTGAAGT TTGGAGCGTA TATAGTGGAC GGTCTGAGAG AGTACAGACA GCCGGTTCTG 
GTGTATATCC CACCACAGGC GGAGCTCCGG GGAGGATCAT GGGTTGTCAT AGACCCTACT 
ATCAATCCTC GCCACATGGA AATGTATGCA GACAAAGACA GCCGTGGCGG TGTGCTGGAG 
CCTGAAGGCA CAGTGGAGAT CAAGTTCCGC AAAAAAGACC TGGTGAAGAC CATGCGACGG 
GTGGACCCTG TGTACATGGG GCTTGCAGAA AGACTGGGAA CCCCAGAGTT GAGTGTGTCT 
GAGCGTAAAG AGCTGGAGAG TAAACTAAAA GAGAGAGAAG AGTTCCTGCT GCCCATATAT 
CATCAAGTAG CTGTTCAGTT CGCTGACCTT CATGACACAC CGGGCCGCAT GCAGGAGAAA 
GGAGTCATCA CTGATATCCT TGAGTGGAGC ACGTCTCGGC AGTTTTTCTA CTGGCGTTTG 
CGGAGGTTAT TGCTGGAGGA AACGGTGAAG AGGAAGATCC AGTGTGCTAA CAGTGAGCTG 
ACGGACGGAC AGGTTCAGGC TATGCTTCGC CGTTGGTTCG TGGAAGCAGA GGGAGCTGTG 
AAAGCGTATT TGTGGGACAG TAATGAAGAT GTGGTGGAGT GGTTGGAGAG GCAGCTGGCA 
GAGGAAGAAG GCGCCAGATC CGTCATCGAC GAGAATATCA AATATATTCG CAGAGATCAC 
ATCCTCAAAC AGATCCGCAG CCTGGTTCAA GCTAACCCTG AGGTGGCTAT GGACTCCATT 
GTACACATGA CCCAGCACAT TTCCCCCACA CAGCGCGCCG AAGTCGTCCG CATCCTCTCC 
ACCATGGACA CCCCAGCCTC CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331874.1
CDS	236..7282
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_021476199.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476199.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAATGTCCT TTTCCTCAAA CCTGAACCAC 
TACGGCATGG TCCACGTGGC CAGCGTTAGT GATGTGCTCC TAGACACCTC CTTTACCCCA 
CCCTGTCAGC GCATGGGAGC CATGGTTTCC TTCCGCTCTT TCCAGGAGTT CACCAGGAAC 
ATAAAGGATG TTCTGAGCTG TTTCTCAGAC TCTCCTCCAT CAACTCCAAC CTTTCCTGAA 
GGGGGGAACC CTGTGCTGTA TAGAGAGGAG GACAGCAAGA GCATTCAAGA TGAGCCCATT 
CATATTCTTA ATGTGGCAAT AAAGACGGAC AGTGATATTG ATGATGATGG GCTGGCGGCC 
ATGTTTCGTG AGTTTACACA GTCTAAGAAA TCTCTGCTGT TTGACCACGG CATCCGAAGA 
CTTACCTTCC TGGTTGCTCA GAAGGATTTC AGAAAGCAAA TCAACTGTGA GGTGGACCAG 
AGGTTCCATA GAGAATTCCC CAAATTTTTT ACATTCCGGG CTCGAGACAA GTTTGAGGAA 
GACCGTATCT ACAGACACTT GGAACCTGCA TTAGCTTTCC AGCTGGAGCT CAACCGCATG 
CGTAATTTTG CACTGACAGC CATCCCCTGT GCCAATCATA AGATGCACCT GTACTTGGGA 
GCTGCCCGTG TGGAAGTAGG CACTGAGGTC ACAGATTACC GCTTCTTTGT GCGGGCAATC 
ATCCGTCACT CAGATTTGGT CACCAAGGAA GCATCATTTG AGTACCTGCA CAACGAGGCA 
GAACGCTTGC TGCTGGAGGC CATGGATGAA CTGGAAGTGG CTTTCAACAA CACCACTGTG 
CGAACCGACT GCAATCACAT CTTCCTCAAC TTCGTTCCAA CAGTCATCAT GGACCCTTCA 
AAGATTGAGG AATCTGTGCG CTCCATGGTC ATGCGCTACG GCAGTCGCTT GTGGAAGCTC 
CGTGTGCTTC AAGCCGAGCT GAAGATCAAC ATCCGATTGA CTCCCACCGG CAAACAGATC 
CCCATCCGCC TCTTCCTGAC CAACGAGAGT GGCTACTACC TGGACATCAG CCTGTACAAG 
GAAGTCACTG ACTCCCGTAC AGGACAGGTG GGGCACAAAG ACCGACAGAT CATGTTCCAG 
GCATATGGAG ATAAGCAGGG TCCTCTTCAT GGCATGCTCA TCAACACACC CTACGTCACA 
AAAGACCTGC TGCAGTCCAA ACGCTTCCAG GCACAGAGCC TCGGCACTAC CTATGTTTAC 
GACTTCCCAG AAATGTTTCG CCAGGCTTTA AGGAAGCTGT GGCAATCCAT GGATGCACAC 
GCCAACTTGC CCAAGTGCCC TCTTCCATCA GAACTGCTCA CCTTCACAGA GCTGGTTCTG 
GATTCTCAGG GTCAGCTGGT GCAGATGAAC CGCCTACCTG GAGGGAATGA GATTGGCATG 
GTAGCATGGC GAATGACCCT CCGGACCCCT GAGTATCCAG CCGGCCGTGA GATTATAGTG 
ATCAGCAATG ACATCACACA TAAGATTGGC TCGTTTGGGC CACAAGAAGA TGTTCTGTTC 
CAGCAAGCTT CAGAAATGGC AAGAGAAAGT GGCATTCCAC GGATCTATAT TGCTGCCAAC 
AGCGGAGCTC GTATAGGCCT GGCAGAGGAG ATCAGACACA TGTTTCATGT GGCCTGGCAG 
GATCCGGTGG ACCCATACAA GGGTTTTAAG TACCTATACC TCACACCACA GGACTATAAG 
AAGGTGTCTG CTCTGAACTC AGTCCACTGT GAACATGTGG AGGATGAGGG GGAATCCAGG 
TATAAAATCA CTGATATCAT TGGGAAAGAG GAAGGGTTGG GTGTGGAGAA CTTGAGAGGT 
TCTGGCATGA TCGCAGGTGA ATCGTCACTG GCGTATGAGG ATATTATCAC CATGAATCTG 
GTGACATGCA GGGCCATTGG AATCGGAGCG TACTTGGTGA GACTGGGCCA AAGAACCATA 
CAGGTGGAAA ACTCTCATAT CATCCTGACT GGAGCCGGAG CTCTCAATAA GGTTCTCGGG 
CGTGAGGTGT ACACTTCCAA TAACCAACTG GGTGGAGTAC AGATCATGCA CAATAACGGA 
GTCACACACA CCAATGTGTG CGATGATTTT GAGGGAGTGT ATACATTGCT GCACTGGCTG 
TCTTACATGC CAAAGAATAT GTCCAGCCCT GTGCCGATAC TCTCTGCTAA AGATCCCATT 
GATCGAGCGA TAGAGTTTGT CCCAACCAAG GCACCTTATG ACCCACGCTG GATGCTAGCT 
GGACGTCCCA ATCAGAACAT TAAGGGTGCA TGGGTGAGTG GGTTCTTCGA CCAGGGCTCT 
TTTCTGGAGA TCATGCAGCC ATGGGCACAG AGTGTTGTCG TAGGACGGGC CAGGTTGGGT 
GGGATCCCAA CTGGAGTAGT TGCCGTGGAA ACCCGATCAG TGGAGCTTTC AATCCCAGCA 
GACCCAGCTA ATCTGGACTC GGAAGCCAAG ATAATCCAGC AGGCTGGGCA AGTGTGGTTC 
CCTGATTCTG CTTTCAAAAC TGCACAAGCC ATTAAAGACT TCAATAGAGA GGGACTTCCG 
CTTATAGTGT TTGCCAACTG GAGAGGTTTT TCTGGAGGCA TGAAAGACAT GTATGACCAG 
GTGCTGAAGT TTGGAGCGTA TATAGTGGAC GGTCTGAGAG AGTACAGACA GCCGGTTCTG 
GTGTATATCC CACCACAGGC GGAGCTCCGG GGAGGATCAT GGGTTGTCAT AGACCCTACT 
ATCAATCCTC GCCACATGGA AATGTATGCA GACAAAGACA GCCGTGGCGG TGTGCTGGAG 
CCTGAAGGCA CAGTGGAGAT CAAGTTCCGC AAAAAAGACC TGGTGAAGAC CATGCGACGG 
GTGGACCCTG TGTACATGGG GCTTGCAGAA AGACTGGGAA CCCCAGAGTT GAGTGTGTCT 
GAGCGTAAAG AGCTGGAGAG TAAACTAAAA GAGAGAGAAG AGTTCCTGCT GCCCATATAT 
CATCAAGTAG CTGTTCAGTT CGCTGACCTT CATGACACAC CGGGCCGCAT GCAGGAGAAA 
GGAGTCATCA CTGATATCCT TGAGTGGAGC ACGTCTCGGC AGTTTTTCTA CTGGCGTTTG 
CGGAGGTTAT TGCTGGAGGA AACGGTGAAG AGGAAGATCC AGTGTGCTAA CAGTGAGCTG 
ACGGACGGAC AGGTTCAGGC TATGCTTCGC CGTTGGTTCG TGGAAGCAGA GGGAGCTGTG 
AAAGCGTATT TGTGGGACAG TAATGAAGAT GTGGTGGAGT GGTTGGAGAG GCAGCTGGCA 
GAGGAAGAAG GCGCCAGATC CGTCATCGAC GAGAATATCA AATATATTCG CAGAGATCAC 
ATCCTCAAAC AGATCCGCAG CCTGGTTCAA GCTAACCCTG AGGTGGCTAT GGACTCCATT 
GTACACATGA CCCAGCACAT TTCCCCCACA CAGCGCGCCG AAGTCGTCCG CATCCTCTCC 
ACCATGGACA CCCCAGCCTC CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_021476200.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331875.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X16, mRNA.

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CloneID	ODa142657
Clone ID Related Accession (Same CDS sequence)	XM_021476200.1
Accession Version	XM_021476200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAATGTCCT TTTCCTCAAA CCTGAACCAC 
TACGGCATGG TCCACGTGGC CAGCGTTAGT GATGTGCTCC TAGACACCTC CTTTACCCCA 
CCCTGTCAGC GCATGGGAGC CATGGTTTCC TTCCGCTCTT TCCAGGAGTT CACCAGGAAC 
ATAAAGGATG TTCTGAGCTG TTTCTCAGAC TCTCCTCCAT CAACTCCAAC CTTTCCTGAA 
GGGGGGAACC CTGTGCTGTA TAGAGAGGAG GACAGCAAGA GCATTCAAGA TGAGCCCATT 
CATATTCTTA ATGTGGCAAT AAAGACGGAC AGTGATATTG ATGATGATGG GCTGGCGGCC 
ATGTTTCGTG AGTTTACACA GTCTAAGAAA TCTCTGCTGT TTGACCACGG CATCCGAAGA 
CTTACCTTCC TGGTTGCTCA GAAGAGAGAA TTCCCCAAAT TTTTTACATT CCGGGCTCGA 
GACAAGTTTG AGGAAGACCG TATCTACAGA CACTTGGAAC CTGCATTAGC TTTCCAGCTG 
GAGCTCAACC GCATGCGTAA TTTTGCACTG ACAGCCATCC CCTGTGCCAA TCATAAGATG 
CACCTGTACT TGGGAGCTGC CCGTGTGGAA GTAGGCACTG AGGTCACAGA TTACCGCTTC 
TTTGTGCGGG CAATCATCCG TCACTCAGAT TTGGTCACCA AGGAAGCATC ATTTGAGTAC 
CTGCACAACG AGGCAGAACG CTTGCTGCTG GAGGCCATGG ATGAACTGGA AGTGGCTTTC 
AACAACACCA CTGTGCGAAC CGACTGCAAT CACATCTTCC TCAACTTCGT TCCAACAGTC 
ATCATGGACC CTTCAAAGAT TGAGGAATCT GTGCGCTCCA TGGTCATGCG CTACGGCAGT 
CGCTTGTGGA AGCTCCGTGT GCTTCAAGCC GAGCTGAAGA TCAACATCCG ATTGACTCCC 
ACCGGCAAAC AGATCCCCAT CCGCCTCTTC CTGACCAACG AGAGTGGCTA CTACCTGGAC 
ATCAGCCTGT ACAAGGAAGT CACTGACTCC CGTACAGGAC AGATCATGTT CCAGGCATAT 
GGAGATAAGC AGGGTCCTCT TCATGGCATG CTCATCAACA CACCCTACGT CACAAAAGAC 
CTGCTGCAGT CCAAACGCTT CCAGGCACAG AGCCTCGGCA CTACCTATGT TTACGACTTC 
CCAGAAATGT TTCGCCAGGC TTTAAGGAAG CTGTGGCAAT CCATGGATGC ACACGCCAAC 
TTGCCCAAGT GCCCTCTTCC ATCAGAACTG CTCACCTTCA CAGAGCTGGT TCTGGATTCT 
CAGGGTCAGC TGGTGCAGAT GAACCGCCTA CCTGGAGGGA ATGAGATTGG CATGGTAGCA 
TGGCGAATGA CCCTCCGGAC CCCTGAGTAT CCAGCCGGCC GTGAGATTAT AGTGATCAGC 
AATGACATCA CACATAAGAT TGGCTCGTTT GGGCCACAAG AAGATGTTCT GTTCCAGCAA 
GCTTCAGAAA TGGCAAGAGA AAGTGGCATT CCACGGATCT ATATTGCTGC CAACAGCGGA 
GCTCGTATAG GCCTGGCAGA GGAGATCAGA CACATGTTTC ATGTGGCCTG GCAGGATCCG 
GTGGACCCAT ACAAGGGTTT TAAGTACCTA TACCTCACAC CACAGGACTA TAAGAAGGTG 
TCTGCTCTGA ACTCAGTCCA CTGTGAACAT GTGGAGGATG AGGGGGAATC CAGGTATAAA 
ATCACTGATA TCATTGGGAA AGAGGAAGGG TTGGGTGTGG AGAACTTGAG AGGTTCTGGC 
ATGATCGCAG GTGAATCGTC ACTGGCGTAT GAGGATATTA TCACCATGAA TCTGGTGACA 
TGCAGGGCCA TTGGAATCGG AGCGTACTTG GTGAGACTGG GCCAAAGAAC CATACAGGTG 
GAAAACTCTC ATATCATCCT GACTGGAGCC GGAGCTCTCA ATAAGGTTCT CGGGCGTGAG 
GTGTACACTT CCAATAACCA ACTGGGTGGA GTACAGATCA TGCACAATAA CGGAGTCACA 
CACACCAATG TGTGCGATGA TTTTGAGGGA GTGTATACAT TGCTGCACTG GCTGTCTTAC 
ATGCCAAAGA ATATGTCCAG CCCTGTGCCG ATACTCTCTG CTAAAGATCC CATTGATCGA 
GCGATAGAGT TTGTCCCAAC CAAGGCACCT TATGACCCAC GCTGGATGCT AGCTGGACGT 
CCCAATCAGA ACATTAAGGG TGCATGGGTG AGTGGGTTCT TCGACCAGGG CTCTTTTCTG 
GAGATCATGC AGCCATGGGC ACAGAGTGTT GTCGTAGGAC GGGCCAGGTT GGGTGGGATC 
CCAACTGGAG TAGTTGCCGT GGAAACCCGA TCAGTGGAGC TTTCAATCCC AGCAGACCCA 
GCTAATCTGG ACTCGGAAGC CAAGATAATC CAGCAGGCTG GGCAAGTGTG GTTCCCTGAT 
TCTGCTTTCA AAACTGCACA AGCCATTAAA GACTTCAATA GAGAGGGACT TCCGCTTATA 
GTGTTTGCCA ACTGGAGAGG TTTTTCTGGA GGCATGAAAG ACATGTATGA CCAGGTGCTG 
AAGTTTGGAG CGTATATAGT GGACGGTCTG AGAGAGTACA GACAGCCGGT TCTGGTGTAT 
ATCCCACCAC AGGCGGAGCT CCGGGGAGGA TCATGGGTTG TCATAGACCC TACTATCAAT 
CCTCGCCACA TGGAAATGTA TGCAGACAAA GACAGCCGTG GCGGTGTGCT GGAGCCTGAA 
GGCACAGTGG AGATCAAGTT CCGCAAAAAA GACCTGGTGA AGACCATGCG ACGGGTGGAC 
CCTGTGTACA TGGGGCTTGC AGAAAGACTG GGAACCCCAG AGTTGAGTGT GTCTGAGCGT 
AAAGAGCTGG AGAGTAAACT AAAAGAGAGA GAAGAGTTCC TGCTGCCCAT ATATCATCAA 
GTAGCTGTTC AGTTCGCTGA CCTTCATGAC ACACCGGGCC GCATGCAGGA GAAAGGAGTC 
ATCACTGATA TCCTTGAGTG GAGCACGTCT CGGCAGTTTT TCTACTGGCG TTTGCGGAGG 
TTATTGCTGG AGGAAACGGT GAAGAGGAAG ATCCAGTGTG CTAACAGTGA GCTGACGGAC 
GGACAGGTTC AGGCTATGCT TCGCCGTTGG TTCGTGGAAG CAGAGGGAGC TGTGAAAGCG 
TATTTGTGGG ACAGTAATGA AGATGTGGTG GAGTGGTTGG AGAGGCAGCT GGCAGAGGAA 
GAAGGCGCCA GATCCGTCAT CGACGAGAAT ATCAAATATA TTCGCAGAGA TCACATCCTC 
AAACAGATCC GCAGCCTGGT TCAAGCTAAC CCTGAGGTGG CTATGGACTC CATTGTACAC 
ATGACCCAGC ACATTTCCCC CACACAGCGC GCCGAAGTCG TCCGCATCCT CTCCACCATG 
GACACCCCAG CCTCCTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331875.1
CDS	237..7217
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_021476200.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476200.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAATGTCCT TTTCCTCAAA CCTGAACCAC 
TACGGCATGG TCCACGTGGC CAGCGTTAGT GATGTGCTCC TAGACACCTC CTTTACCCCA 
CCCTGTCAGC GCATGGGAGC CATGGTTTCC TTCCGCTCTT TCCAGGAGTT CACCAGGAAC 
ATAAAGGATG TTCTGAGCTG TTTCTCAGAC TCTCCTCCAT CAACTCCAAC CTTTCCTGAA 
GGGGGGAACC CTGTGCTGTA TAGAGAGGAG GACAGCAAGA GCATTCAAGA TGAGCCCATT 
CATATTCTTA ATGTGGCAAT AAAGACGGAC AGTGATATTG ATGATGATGG GCTGGCGGCC 
ATGTTTCGTG AGTTTACACA GTCTAAGAAA TCTCTGCTGT TTGACCACGG CATCCGAAGA 
CTTACCTTCC TGGTTGCTCA GAAGAGAGAA TTCCCCAAAT TTTTTACATT CCGGGCTCGA 
GACAAGTTTG AGGAAGACCG TATCTACAGA CACTTGGAAC CTGCATTAGC TTTCCAGCTG 
GAGCTCAACC GCATGCGTAA TTTTGCACTG ACAGCCATCC CCTGTGCCAA TCATAAGATG 
CACCTGTACT TGGGAGCTGC CCGTGTGGAA GTAGGCACTG AGGTCACAGA TTACCGCTTC 
TTTGTGCGGG CAATCATCCG TCACTCAGAT TTGGTCACCA AGGAAGCATC ATTTGAGTAC 
CTGCACAACG AGGCAGAACG CTTGCTGCTG GAGGCCATGG ATGAACTGGA AGTGGCTTTC 
AACAACACCA CTGTGCGAAC CGACTGCAAT CACATCTTCC TCAACTTCGT TCCAACAGTC 
ATCATGGACC CTTCAAAGAT TGAGGAATCT GTGCGCTCCA TGGTCATGCG CTACGGCAGT 
CGCTTGTGGA AGCTCCGTGT GCTTCAAGCC GAGCTGAAGA TCAACATCCG ATTGACTCCC 
ACCGGCAAAC AGATCCCCAT CCGCCTCTTC CTGACCAACG AGAGTGGCTA CTACCTGGAC 
ATCAGCCTGT ACAAGGAAGT CACTGACTCC CGTACAGGAC AGATCATGTT CCAGGCATAT 
GGAGATAAGC AGGGTCCTCT TCATGGCATG CTCATCAACA CACCCTACGT CACAAAAGAC 
CTGCTGCAGT CCAAACGCTT CCAGGCACAG AGCCTCGGCA CTACCTATGT TTACGACTTC 
CCAGAAATGT TTCGCCAGGC TTTAAGGAAG CTGTGGCAAT CCATGGATGC ACACGCCAAC 
TTGCCCAAGT GCCCTCTTCC ATCAGAACTG CTCACCTTCA CAGAGCTGGT TCTGGATTCT 
CAGGGTCAGC TGGTGCAGAT GAACCGCCTA CCTGGAGGGA ATGAGATTGG CATGGTAGCA 
TGGCGAATGA CCCTCCGGAC CCCTGAGTAT CCAGCCGGCC GTGAGATTAT AGTGATCAGC 
AATGACATCA CACATAAGAT TGGCTCGTTT GGGCCACAAG AAGATGTTCT GTTCCAGCAA 
GCTTCAGAAA TGGCAAGAGA AAGTGGCATT CCACGGATCT ATATTGCTGC CAACAGCGGA 
GCTCGTATAG GCCTGGCAGA GGAGATCAGA CACATGTTTC ATGTGGCCTG GCAGGATCCG 
GTGGACCCAT ACAAGGGTTT TAAGTACCTA TACCTCACAC CACAGGACTA TAAGAAGGTG 
TCTGCTCTGA ACTCAGTCCA CTGTGAACAT GTGGAGGATG AGGGGGAATC CAGGTATAAA 
ATCACTGATA TCATTGGGAA AGAGGAAGGG TTGGGTGTGG AGAACTTGAG AGGTTCTGGC 
ATGATCGCAG GTGAATCGTC ACTGGCGTAT GAGGATATTA TCACCATGAA TCTGGTGACA 
TGCAGGGCCA TTGGAATCGG AGCGTACTTG GTGAGACTGG GCCAAAGAAC CATACAGGTG 
GAAAACTCTC ATATCATCCT GACTGGAGCC GGAGCTCTCA ATAAGGTTCT CGGGCGTGAG 
GTGTACACTT CCAATAACCA ACTGGGTGGA GTACAGATCA TGCACAATAA CGGAGTCACA 
CACACCAATG TGTGCGATGA TTTTGAGGGA GTGTATACAT TGCTGCACTG GCTGTCTTAC 
ATGCCAAAGA ATATGTCCAG CCCTGTGCCG ATACTCTCTG CTAAAGATCC CATTGATCGA 
GCGATAGAGT TTGTCCCAAC CAAGGCACCT TATGACCCAC GCTGGATGCT AGCTGGACGT 
CCCAATCAGA ACATTAAGGG TGCATGGGTG AGTGGGTTCT TCGACCAGGG CTCTTTTCTG 
GAGATCATGC AGCCATGGGC ACAGAGTGTT GTCGTAGGAC GGGCCAGGTT GGGTGGGATC 
CCAACTGGAG TAGTTGCCGT GGAAACCCGA TCAGTGGAGC TTTCAATCCC AGCAGACCCA 
GCTAATCTGG ACTCGGAAGC CAAGATAATC CAGCAGGCTG GGCAAGTGTG GTTCCCTGAT 
TCTGCTTTCA AAACTGCACA AGCCATTAAA GACTTCAATA GAGAGGGACT TCCGCTTATA 
GTGTTTGCCA ACTGGAGAGG TTTTTCTGGA GGCATGAAAG ACATGTATGA CCAGGTGCTG 
AAGTTTGGAG CGTATATAGT GGACGGTCTG AGAGAGTACA GACAGCCGGT TCTGGTGTAT 
ATCCCACCAC AGGCGGAGCT CCGGGGAGGA TCATGGGTTG TCATAGACCC TACTATCAAT 
CCTCGCCACA TGGAAATGTA TGCAGACAAA GACAGCCGTG GCGGTGTGCT GGAGCCTGAA 
GGCACAGTGG AGATCAAGTT CCGCAAAAAA GACCTGGTGA AGACCATGCG ACGGGTGGAC 
CCTGTGTACA TGGGGCTTGC AGAAAGACTG GGAACCCCAG AGTTGAGTGT GTCTGAGCGT 
AAAGAGCTGG AGAGTAAACT AAAAGAGAGA GAAGAGTTCC TGCTGCCCAT ATATCATCAA 
GTAGCTGTTC AGTTCGCTGA CCTTCATGAC ACACCGGGCC GCATGCAGGA GAAAGGAGTC 
ATCACTGATA TCCTTGAGTG GAGCACGTCT CGGCAGTTTT TCTACTGGCG TTTGCGGAGG 
TTATTGCTGG AGGAAACGGT GAAGAGGAAG ATCCAGTGTG CTAACAGTGA GCTGACGGAC 
GGACAGGTTC AGGCTATGCT TCGCCGTTGG TTCGTGGAAG CAGAGGGAGC TGTGAAAGCG 
TATTTGTGGG ACAGTAATGA AGATGTGGTG GAGTGGTTGG AGAGGCAGCT GGCAGAGGAA 
GAAGGCGCCA GATCCGTCAT CGACGAGAAT ATCAAATATA TTCGCAGAGA TCACATCCTC 
AAACAGATCC GCAGCCTGGT TCAAGCTAAC CCTGAGGTGG CTATGGACTC CATTGTACAC 
ATGACCCAGC ACATTTCCCC CACACAGCGC GCCGAAGTCG TCCGCATCCT CTCCACCATG 
GACACCCCAG CCTCCTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_021476193.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331868.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X2, mRNA.

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CloneID	ODa43079
Clone ID Related Accession (Same CDS sequence)	XM_021476193.1 , XM_021476192.1
Accession Version	XM_021476193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGC ATTCAGCAAT CCCATCAATA CAGACAATTT TGAAAACAAC ACTGGCCGAA 
ATGCAGCCCC AAACCAGGAC AAACCAGAGA CTCCAACATG TTTCACTGAA GGCTATTGAG 
GAATCTGTGC GCTCCATGGT CATGCGCTAC GGCAGTCGCT TGTGGAAGCT CCGTGTGCTT 
CAAGCCGAGC TGAAGATCAA CATCCGATTG ACTCCCACCG GCAAACAGAT CCCCATCCGC 
CTCTTCCTGA CCAACGAGAG TGGCTACTAC CTGGACATCA GCCTGTACAA GGAAGTCACT 
GACTCCCGTA CAGGACAGGT GGGGCACAAA GACCGACAGA TCATGTTCCA GGCATATGGA 
GATAAGCAGG GTCCTCTTCA TGGCATGCTC ATCAACACAC CCTACGTCAC AAAAGACCTG 
CTGCAGTCCA AACGCTTCCA GGCACAGAGC CTCGGCACTA CCTATGTTTA CGACTTCCCA 
GAAATGTTTC GCCAGGCTTT AAGGAAGCTG TGGCAATCCA TGGATGCACA CGCCAACTTG 
CCCAAGTGCC CTCTTCCATC AGAACTGCTC ACCTTCACAG AGCTGGTTCT GGATTCTCAG 
GGTCAGCTGG TGCAGATGAA CCGCCTACCT GGAGGGAATG AGATTGGCAT GGTAGCATGG 
CGAATGACCC TCCGGACCCC TGAGTATCCA GCCGGCCGTG AGATTATAGT GATCAGCAAT 
GACATCACAC ATAAGATTGG CTCGTTTGGG CCACAAGAAG ATGTTCTGTT CCAGCAAGCT 
TCAGAAATGG CAAGAGAAAG TGGCATTCCA CGGATCTATA TTGCTGCCAA CAGCGGAGCT 
CGTATAGGCC TGGCAGAGGA GATCAGACAC ATGTTTCATG TGGCCTGGCA GGATCCGGTG 
GACCCATACA AGGGTTTTAA GTACCTATAC CTCACACCAC AGGACTATAA GAAGGTGTCT 
GCTCTGAACT CAGTCCACTG TGAACATGTG GAGGATGAGG GGGAATCCAG GTATAAAATC 
ACTGATATCA TTGGGAAAGA GGAAGGGTTG GGTGTGGAGA ACTTGAGAGG TTCTGGCATG 
ATCGCAGGTG AATCGTCACT GGCGTATGAG GATATTATCA CCATGAATCT GGTGACATGC 
AGGGCCATTG GAATCGGAGC GTACTTGGTG AGACTGGGCC AAAGAACCAT ACAGGTGGAA 
AACTCTCATA TCATCCTGAC TGGAGCCGGA GCTCTCAATA AGGTTCTCGG GCGTGAGGTG 
TACACTTCCA ATAACCAACT GGGTGGAGTA CAGATCATGC ACAATAACGG AGTCACACAC 
ACCAATGTGT GCGATGATTT TGAGGGAGTG TATACATTGC TGCACTGGCT GTCTTACATG 
CCAAAGAATA TGTCCAGCCC TGTGCCGATA CTCTCTGCTA AAGATCCCAT TGATCGAGCG 
ATAGAGTTTG TCCCAACCAA GGCACCTTAT GACCCACGCT GGATGCTAGC TGGACGTCCC 
AATCAGAACA TTAAGGGTGC ATGGGTGAGT GGGTTCTTCG ACCAGGGCTC TTTTCTGGAG 
ATCATGCAGC CATGGGCACA GAGTGTTGTC GTAGGACGGG CCAGGTTGGG TGGGATCCCA 
ACTGGAGTAG TTGCCGTGGA AACCCGATCA GTGGAGCTTT CAATCCCAGC AGACCCAGCT 
AATCTGGACT CGGAAGCCAA GATAATCCAG CAGGCTGGGC AAGTGTGGTT CCCTGATTCT 
GCTTTCAAAA CTGCACAAGC CATTAAAGAC TTCAATAGAG AGGGACTTCC GCTTATAGTG 
TTTGCCAACT GGAGAGGTTT TTCTGGAGGC ATGAAAGACA TGTATGACCA GGTGCTGAAG 
TTTGGAGCGT ATATAGTGGA CGGTCTGAGA GAGTACAGAC AGCCGGTTCT GGTGTATATC 
CCACCACAGG CGGAGCTCCG GGGAGGATCA TGGGTTGTCA TAGACCCTAC TATCAATCCT 
CGCCACATGG AAATGTATGC AGACAAAGAC AGCCGTGGCG GTGTGCTGGA GCCTGAAGGC 
ACAGTGGAGA TCAAGTTCCG CAAAAAAGAC CTGGTGAAGA CCATGCGACG GGTGGACCCT 
GTGTACATGG GGCTTGCAGA AAGACTGGGA ACCCCAGAGT TGAGTGTGTC TGAGCGTAAA 
GAGCTGGAGA GTAAACTAAA AGAGAGAGAA GAGTTCCTGC TGCCCATATA TCATCAAGTA 
GCTGTTCAGT TCGCTGACCT TCATGACACA CCGGGCCGCA TGCAGGAGAA AGGAGTCATC 
ACTGATATCC TTGAGTGGAG CACGTCTCGG CAGTTTTTCT ACTGGCGTTT GCGGAGGTTA 
TTGCTGGAGG AAACGGTGAA GAGGAAGATC CAGTGTGCTA ACAGTGAGCT GACGGACGGA 
CAGGTTCAGG CTATGCTTCG CCGTTGGTTC GTGGAAGCAG AGGGAGCTGT GAAAGCGTAT 
TTGTGGGACA GTAATGAAGA TGTGGTGGAG TGGTTGGAGA GGCAGCTGGC AGAGGAAGAA 
GGCGCCAGAT CCGTCATCGA CGAGAATATC AAATATATTC GCAGAGATCA CATCCTCAAA 
CAGATCCGCA GCCTGGTTCA AGCTAACCCT GAGGTGGCTA TGGACTCCAT TGTACACATG 
ACCCAGCACA TTTCCCCCAC ACAGCGCGCC GAAGTCGTCC GCATCCTCTC CACCATGGAC 
ACCCCAGCCT CCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331868.1
CDS	105..7382
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRKLSAPLQDYKPPANTANTSGATEAASDSAESEDRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKHSAIPSIQTILKTTLAEMQPQTRTNQRLQHVSLKAIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_021476193.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476193.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGC ATTCAGCAAT CCCATCAATA CAGACAATTT TGAAAACAAC ACTGGCCGAA 
ATGCAGCCCC AAACCAGGAC AAACCAGAGA CTCCAACATG TTTCACTGAA GGCTATTGAG 
GAATCTGTGC GCTCCATGGT CATGCGCTAC GGCAGTCGCT TGTGGAAGCT CCGTGTGCTT 
CAAGCCGAGC TGAAGATCAA CATCCGATTG ACTCCCACCG GCAAACAGAT CCCCATCCGC 
CTCTTCCTGA CCAACGAGAG TGGCTACTAC CTGGACATCA GCCTGTACAA GGAAGTCACT 
GACTCCCGTA CAGGACAGGT GGGGCACAAA GACCGACAGA TCATGTTCCA GGCATATGGA 
GATAAGCAGG GTCCTCTTCA TGGCATGCTC ATCAACACAC CCTACGTCAC AAAAGACCTG 
CTGCAGTCCA AACGCTTCCA GGCACAGAGC CTCGGCACTA CCTATGTTTA CGACTTCCCA 
GAAATGTTTC GCCAGGCTTT AAGGAAGCTG TGGCAATCCA TGGATGCACA CGCCAACTTG 
CCCAAGTGCC CTCTTCCATC AGAACTGCTC ACCTTCACAG AGCTGGTTCT GGATTCTCAG 
GGTCAGCTGG TGCAGATGAA CCGCCTACCT GGAGGGAATG AGATTGGCAT GGTAGCATGG 
CGAATGACCC TCCGGACCCC TGAGTATCCA GCCGGCCGTG AGATTATAGT GATCAGCAAT 
GACATCACAC ATAAGATTGG CTCGTTTGGG CCACAAGAAG ATGTTCTGTT CCAGCAAGCT 
TCAGAAATGG CAAGAGAAAG TGGCATTCCA CGGATCTATA TTGCTGCCAA CAGCGGAGCT 
CGTATAGGCC TGGCAGAGGA GATCAGACAC ATGTTTCATG TGGCCTGGCA GGATCCGGTG 
GACCCATACA AGGGTTTTAA GTACCTATAC CTCACACCAC AGGACTATAA GAAGGTGTCT 
GCTCTGAACT CAGTCCACTG TGAACATGTG GAGGATGAGG GGGAATCCAG GTATAAAATC 
ACTGATATCA TTGGGAAAGA GGAAGGGTTG GGTGTGGAGA ACTTGAGAGG TTCTGGCATG 
ATCGCAGGTG AATCGTCACT GGCGTATGAG GATATTATCA CCATGAATCT GGTGACATGC 
AGGGCCATTG GAATCGGAGC GTACTTGGTG AGACTGGGCC AAAGAACCAT ACAGGTGGAA 
AACTCTCATA TCATCCTGAC TGGAGCCGGA GCTCTCAATA AGGTTCTCGG GCGTGAGGTG 
TACACTTCCA ATAACCAACT GGGTGGAGTA CAGATCATGC ACAATAACGG AGTCACACAC 
ACCAATGTGT GCGATGATTT TGAGGGAGTG TATACATTGC TGCACTGGCT GTCTTACATG 
CCAAAGAATA TGTCCAGCCC TGTGCCGATA CTCTCTGCTA AAGATCCCAT TGATCGAGCG 
ATAGAGTTTG TCCCAACCAA GGCACCTTAT GACCCACGCT GGATGCTAGC TGGACGTCCC 
AATCAGAACA TTAAGGGTGC ATGGGTGAGT GGGTTCTTCG ACCAGGGCTC TTTTCTGGAG 
ATCATGCAGC CATGGGCACA GAGTGTTGTC GTAGGACGGG CCAGGTTGGG TGGGATCCCA 
ACTGGAGTAG TTGCCGTGGA AACCCGATCA GTGGAGCTTT CAATCCCAGC AGACCCAGCT 
AATCTGGACT CGGAAGCCAA GATAATCCAG CAGGCTGGGC AAGTGTGGTT CCCTGATTCT 
GCTTTCAAAA CTGCACAAGC CATTAAAGAC TTCAATAGAG AGGGACTTCC GCTTATAGTG 
TTTGCCAACT GGAGAGGTTT TTCTGGAGGC ATGAAAGACA TGTATGACCA GGTGCTGAAG 
TTTGGAGCGT ATATAGTGGA CGGTCTGAGA GAGTACAGAC AGCCGGTTCT GGTGTATATC 
CCACCACAGG CGGAGCTCCG GGGAGGATCA TGGGTTGTCA TAGACCCTAC TATCAATCCT 
CGCCACATGG AAATGTATGC AGACAAAGAC AGCCGTGGCG GTGTGCTGGA GCCTGAAGGC 
ACAGTGGAGA TCAAGTTCCG CAAAAAAGAC CTGGTGAAGA CCATGCGACG GGTGGACCCT 
GTGTACATGG GGCTTGCAGA AAGACTGGGA ACCCCAGAGT TGAGTGTGTC TGAGCGTAAA 
GAGCTGGAGA GTAAACTAAA AGAGAGAGAA GAGTTCCTGC TGCCCATATA TCATCAAGTA 
GCTGTTCAGT TCGCTGACCT TCATGACACA CCGGGCCGCA TGCAGGAGAA AGGAGTCATC 
ACTGATATCC TTGAGTGGAG CACGTCTCGG CAGTTTTTCT ACTGGCGTTT GCGGAGGTTA 
TTGCTGGAGG AAACGGTGAA GAGGAAGATC CAGTGTGCTA ACAGTGAGCT GACGGACGGA 
CAGGTTCAGG CTATGCTTCG CCGTTGGTTC GTGGAAGCAG AGGGAGCTGT GAAAGCGTAT 
TTGTGGGACA GTAATGAAGA TGTGGTGGAG TGGTTGGAGA GGCAGCTGGC AGAGGAAGAA 
GGCGCCAGAT CCGTCATCGA CGAGAATATC AAATATATTC GCAGAGATCA CATCCTCAAA 
CAGATCCGCA GCCTGGTTCA AGCTAACCCT GAGGTGGCTA TGGACTCCAT TGTACACATG 
ACCCAGCACA TTTCCCCCAC ACAGCGCGCC GAAGTCGTCC GCATCCTCTC CACCATGGAC 
ACCCCAGCCT CCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_021476192.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331867.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X1, mRNA.

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CloneID	ODa43079
Clone ID Related Accession (Same CDS sequence)	XM_021476193.1 , XM_021476192.1
Accession Version	XM_021476192.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGC ATTCAGCAAT CCCATCAATA CAGACAATTT TGAAAACAAC ACTGGCCGAA 
ATGCAGCCCC AAACCAGGAC AAACCAGAGA CTCCAACATG TTTCACTGAA GGCTATTGAG 
GAATCTGTGC GCTCCATGGT CATGCGCTAC GGCAGTCGCT TGTGGAAGCT CCGTGTGCTT 
CAAGCCGAGC TGAAGATCAA CATCCGATTG ACTCCCACCG GCAAACAGAT CCCCATCCGC 
CTCTTCCTGA CCAACGAGAG TGGCTACTAC CTGGACATCA GCCTGTACAA GGAAGTCACT 
GACTCCCGTA CAGGACAGGT GGGGCACAAA GACCGACAGA TCATGTTCCA GGCATATGGA 
GATAAGCAGG GTCCTCTTCA TGGCATGCTC ATCAACACAC CCTACGTCAC AAAAGACCTG 
CTGCAGTCCA AACGCTTCCA GGCACAGAGC CTCGGCACTA CCTATGTTTA CGACTTCCCA 
GAAATGTTTC GCCAGGCTTT AAGGAAGCTG TGGCAATCCA TGGATGCACA CGCCAACTTG 
CCCAAGTGCC CTCTTCCATC AGAACTGCTC ACCTTCACAG AGCTGGTTCT GGATTCTCAG 
GGTCAGCTGG TGCAGATGAA CCGCCTACCT GGAGGGAATG AGATTGGCAT GGTAGCATGG 
CGAATGACCC TCCGGACCCC TGAGTATCCA GCCGGCCGTG AGATTATAGT GATCAGCAAT 
GACATCACAC ATAAGATTGG CTCGTTTGGG CCACAAGAAG ATGTTCTGTT CCAGCAAGCT 
TCAGAAATGG CAAGAGAAAG TGGCATTCCA CGGATCTATA TTGCTGCCAA CAGCGGAGCT 
CGTATAGGCC TGGCAGAGGA GATCAGACAC ATGTTTCATG TGGCCTGGCA GGATCCGGTG 
GACCCATACA AGGGTTTTAA GTACCTATAC CTCACACCAC AGGACTATAA GAAGGTGTCT 
GCTCTGAACT CAGTCCACTG TGAACATGTG GAGGATGAGG GGGAATCCAG GTATAAAATC 
ACTGATATCA TTGGGAAAGA GGAAGGGTTG GGTGTGGAGA ACTTGAGAGG TTCTGGCATG 
ATCGCAGGTG AATCGTCACT GGCGTATGAG GATATTATCA CCATGAATCT GGTGACATGC 
AGGGCCATTG GAATCGGAGC GTACTTGGTG AGACTGGGCC AAAGAACCAT ACAGGTGGAA 
AACTCTCATA TCATCCTGAC TGGAGCCGGA GCTCTCAATA AGGTTCTCGG GCGTGAGGTG 
TACACTTCCA ATAACCAACT GGGTGGAGTA CAGATCATGC ACAATAACGG AGTCACACAC 
ACCAATGTGT GCGATGATTT TGAGGGAGTG TATACATTGC TGCACTGGCT GTCTTACATG 
CCAAAGAATA TGTCCAGCCC TGTGCCGATA CTCTCTGCTA AAGATCCCAT TGATCGAGCG 
ATAGAGTTTG TCCCAACCAA GGCACCTTAT GACCCACGCT GGATGCTAGC TGGACGTCCC 
AATCAGAACA TTAAGGGTGC ATGGGTGAGT GGGTTCTTCG ACCAGGGCTC TTTTCTGGAG 
ATCATGCAGC CATGGGCACA GAGTGTTGTC GTAGGACGGG CCAGGTTGGG TGGGATCCCA 
ACTGGAGTAG TTGCCGTGGA AACCCGATCA GTGGAGCTTT CAATCCCAGC AGACCCAGCT 
AATCTGGACT CGGAAGCCAA GATAATCCAG CAGGCTGGGC AAGTGTGGTT CCCTGATTCT 
GCTTTCAAAA CTGCACAAGC CATTAAAGAC TTCAATAGAG AGGGACTTCC GCTTATAGTG 
TTTGCCAACT GGAGAGGTTT TTCTGGAGGC ATGAAAGACA TGTATGACCA GGTGCTGAAG 
TTTGGAGCGT ATATAGTGGA CGGTCTGAGA GAGTACAGAC AGCCGGTTCT GGTGTATATC 
CCACCACAGG CGGAGCTCCG GGGAGGATCA TGGGTTGTCA TAGACCCTAC TATCAATCCT 
CGCCACATGG AAATGTATGC AGACAAAGAC AGCCGTGGCG GTGTGCTGGA GCCTGAAGGC 
ACAGTGGAGA TCAAGTTCCG CAAAAAAGAC CTGGTGAAGA CCATGCGACG GGTGGACCCT 
GTGTACATGG GGCTTGCAGA AAGACTGGGA ACCCCAGAGT TGAGTGTGTC TGAGCGTAAA 
GAGCTGGAGA GTAAACTAAA AGAGAGAGAA GAGTTCCTGC TGCCCATATA TCATCAAGTA 
GCTGTTCAGT TCGCTGACCT TCATGACACA CCGGGCCGCA TGCAGGAGAA AGGAGTCATC 
ACTGATATCC TTGAGTGGAG CACGTCTCGG CAGTTTTTCT ACTGGCGTTT GCGGAGGTTA 
TTGCTGGAGG AAACGGTGAA GAGGAAGATC CAGTGTGCTA ACAGTGAGCT GACGGACGGA 
CAGGTTCAGG CTATGCTTCG CCGTTGGTTC GTGGAAGCAG AGGGAGCTGT GAAAGCGTAT 
TTGTGGGACA GTAATGAAGA TGTGGTGGAG TGGTTGGAGA GGCAGCTGGC AGAGGAAGAA 
GGCGCCAGAT CCGTCATCGA CGAGAATATC AAATATATTC GCAGAGATCA CATCCTCAAA 
CAGATCCGCA GCCTGGTTCA AGCTAACCCT GAGGTGGCTA TGGACTCCAT TGTACACATG 
ACCCAGCACA TTTCCCCCAC ACAGCGCGCC GAAGTCGTCC GCATCCTCTC CACCATGGAC 
ACCCCAGCCT CCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331867.1
CDS	237..7514
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRKLSAPLQDYKPPANTANTSGATEAASDSAESEDRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKHSAIPSIQTILKTTLAEMQPQTRTNQRLQHVSLKAIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_021476192.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476192.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGC ATTCAGCAAT CCCATCAATA CAGACAATTT TGAAAACAAC ACTGGCCGAA 
ATGCAGCCCC AAACCAGGAC AAACCAGAGA CTCCAACATG TTTCACTGAA GGCTATTGAG 
GAATCTGTGC GCTCCATGGT CATGCGCTAC GGCAGTCGCT TGTGGAAGCT CCGTGTGCTT 
CAAGCCGAGC TGAAGATCAA CATCCGATTG ACTCCCACCG GCAAACAGAT CCCCATCCGC 
CTCTTCCTGA CCAACGAGAG TGGCTACTAC CTGGACATCA GCCTGTACAA GGAAGTCACT 
GACTCCCGTA CAGGACAGGT GGGGCACAAA GACCGACAGA TCATGTTCCA GGCATATGGA 
GATAAGCAGG GTCCTCTTCA TGGCATGCTC ATCAACACAC CCTACGTCAC AAAAGACCTG 
CTGCAGTCCA AACGCTTCCA GGCACAGAGC CTCGGCACTA CCTATGTTTA CGACTTCCCA 
GAAATGTTTC GCCAGGCTTT AAGGAAGCTG TGGCAATCCA TGGATGCACA CGCCAACTTG 
CCCAAGTGCC CTCTTCCATC AGAACTGCTC ACCTTCACAG AGCTGGTTCT GGATTCTCAG 
GGTCAGCTGG TGCAGATGAA CCGCCTACCT GGAGGGAATG AGATTGGCAT GGTAGCATGG 
CGAATGACCC TCCGGACCCC TGAGTATCCA GCCGGCCGTG AGATTATAGT GATCAGCAAT 
GACATCACAC ATAAGATTGG CTCGTTTGGG CCACAAGAAG ATGTTCTGTT CCAGCAAGCT 
TCAGAAATGG CAAGAGAAAG TGGCATTCCA CGGATCTATA TTGCTGCCAA CAGCGGAGCT 
CGTATAGGCC TGGCAGAGGA GATCAGACAC ATGTTTCATG TGGCCTGGCA GGATCCGGTG 
GACCCATACA AGGGTTTTAA GTACCTATAC CTCACACCAC AGGACTATAA GAAGGTGTCT 
GCTCTGAACT CAGTCCACTG TGAACATGTG GAGGATGAGG GGGAATCCAG GTATAAAATC 
ACTGATATCA TTGGGAAAGA GGAAGGGTTG GGTGTGGAGA ACTTGAGAGG TTCTGGCATG 
ATCGCAGGTG AATCGTCACT GGCGTATGAG GATATTATCA CCATGAATCT GGTGACATGC 
AGGGCCATTG GAATCGGAGC GTACTTGGTG AGACTGGGCC AAAGAACCAT ACAGGTGGAA 
AACTCTCATA TCATCCTGAC TGGAGCCGGA GCTCTCAATA AGGTTCTCGG GCGTGAGGTG 
TACACTTCCA ATAACCAACT GGGTGGAGTA CAGATCATGC ACAATAACGG AGTCACACAC 
ACCAATGTGT GCGATGATTT TGAGGGAGTG TATACATTGC TGCACTGGCT GTCTTACATG 
CCAAAGAATA TGTCCAGCCC TGTGCCGATA CTCTCTGCTA AAGATCCCAT TGATCGAGCG 
ATAGAGTTTG TCCCAACCAA GGCACCTTAT GACCCACGCT GGATGCTAGC TGGACGTCCC 
AATCAGAACA TTAAGGGTGC ATGGGTGAGT GGGTTCTTCG ACCAGGGCTC TTTTCTGGAG 
ATCATGCAGC CATGGGCACA GAGTGTTGTC GTAGGACGGG CCAGGTTGGG TGGGATCCCA 
ACTGGAGTAG TTGCCGTGGA AACCCGATCA GTGGAGCTTT CAATCCCAGC AGACCCAGCT 
AATCTGGACT CGGAAGCCAA GATAATCCAG CAGGCTGGGC AAGTGTGGTT CCCTGATTCT 
GCTTTCAAAA CTGCACAAGC CATTAAAGAC TTCAATAGAG AGGGACTTCC GCTTATAGTG 
TTTGCCAACT GGAGAGGTTT TTCTGGAGGC ATGAAAGACA TGTATGACCA GGTGCTGAAG 
TTTGGAGCGT ATATAGTGGA CGGTCTGAGA GAGTACAGAC AGCCGGTTCT GGTGTATATC 
CCACCACAGG CGGAGCTCCG GGGAGGATCA TGGGTTGTCA TAGACCCTAC TATCAATCCT 
CGCCACATGG AAATGTATGC AGACAAAGAC AGCCGTGGCG GTGTGCTGGA GCCTGAAGGC 
ACAGTGGAGA TCAAGTTCCG CAAAAAAGAC CTGGTGAAGA CCATGCGACG GGTGGACCCT 
GTGTACATGG GGCTTGCAGA AAGACTGGGA ACCCCAGAGT TGAGTGTGTC TGAGCGTAAA 
GAGCTGGAGA GTAAACTAAA AGAGAGAGAA GAGTTCCTGC TGCCCATATA TCATCAAGTA 
GCTGTTCAGT TCGCTGACCT TCATGACACA CCGGGCCGCA TGCAGGAGAA AGGAGTCATC 
ACTGATATCC TTGAGTGGAG CACGTCTCGG CAGTTTTTCT ACTGGCGTTT GCGGAGGTTA 
TTGCTGGAGG AAACGGTGAA GAGGAAGATC CAGTGTGCTA ACAGTGAGCT GACGGACGGA 
CAGGTTCAGG CTATGCTTCG CCGTTGGTTC GTGGAAGCAG AGGGAGCTGT GAAAGCGTAT 
TTGTGGGACA GTAATGAAGA TGTGGTGGAG TGGTTGGAGA GGCAGCTGGC AGAGGAAGAA 
GGCGCCAGAT CCGTCATCGA CGAGAATATC AAATATATTC GCAGAGATCA CATCCTCAAA 
CAGATCCGCA GCCTGGTTCA AGCTAACCCT GAGGTGGCTA TGGACTCCAT TGTACACATG 
ACCCAGCACA TTTCCCCCAC ACAGCGCGCC GAAGTCGTCC GCATCCTCTC CACCATGGAC 
ACCCCAGCCT CCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_017356111.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211600.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X3, mRNA.

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CloneID	ODa59092
Clone ID Related Accession (Same CDS sequence)	XM_017356111.1 , XM_017356111.2
Accession Version	XM_017356111.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGA TTGAGGAATC TGTGCGCTCC ATGGTCATGC GCTACGGCAG TCGCTTGTGG 
AAGCTCCGTG TGCTTCAAGC CGAGCTGAAG ATCAACATCC GATTGACTCC CACCGGCAAA 
CAGATCCCCA TCCGCCTCTT CCTGACCAAC GAGAGTGGCT ACTACCTGGA CATCAGCCTG 
TACAAGGAAG TCACTGACTC CCGTACAGGA CAGATCATGT TCCAGGCATA TGGAGATAAG 
CAGGGTCCTC TTCATGGCAT GCTCATCAAC ACACCCTACG TCACAAAAGA CCTGCTGCAG 
TCCAAACGCT TCCAGGCACA GAGCCTCGGC ACTACCTATG TTTACGACTT CCCAGAAATG 
TTTCGCCAGG CTTTAAGGAA GCTGTGGCAA TCCATGGATG CACACGCCAA CTTGCCCAAG 
TGCCCTCTTC CATCAGAACT GCTCACCTTC ACAGAGCTGG TTCTGGATTC TCAGGGTCAG 
CTGGTGCAGA TGAACCGCCT ACCTGGAGGG AATGAGATTG GCATGGTAGC ATGGCGAATG 
ACCCTCCGGA CCCCTGAGTA TCCAGCCGGC CGTGAGATTA TAGTGATCAG CAATGACATC 
ACACATAAGA TTGGCTCGTT TGGGCCACAA GAAGATGTTC TGTTCCAGCA AGCTTCAGAA 
ATGGCAAGAG AAAGTGGCAT TCCACGGATC TATATTGCTG CCAACAGCGG AGCTCGTATA 
GGCCTGGCAG AGGAGATCAG ACACATGTTT CATGTGGCCT GGCAGGATCC GGTGGACCCA 
TACAAGGGTT TTAAGTACCT ATACCTCACA CCACAGGACT ATAAGAAGGT GTCTGCTCTG 
AACTCAGTCC ACTGTGAACA TGTGGAGGAT GAGGGGGAAT CCAGGTATAA AATCACTGAT 
ATCATTGGGA AAGAGGAAGG GTTGGGTGTG GAGAACTTGA GAGGTTCTGG CATGATCGCA 
GGTGAATCGT CACTGGCGTA TGAGGATATT ATCACCATGA ATCTGGTGAC ATGCAGGGCC 
ATTGGAATCG GAGCGTACTT GGTGAGACTG GGCCAAAGAA CCATACAGGT GGAAAACTCT 
CATATCATCC TGACTGGAGC CGGAGCTCTC AATAAGGTTC TCGGGCGTGA GGTGTACACT 
TCCAATAACC AACTGGGTGG AGTACAGATC ATGCACAATA ACGGAGTCAC ACACACCAAT 
GTGTGCGATG ATTTTGAGGG AGTGTATACA TTGCTGCACT GGCTGTCTTA CATGCCAAAG 
AATATGTCCA GCCCTGTGCC GATACTCTCT GCTAAAGATC CCATTGATCG AGCGATAGAG 
TTTGTCCCAA CCAAGGCACC TTATGACCCA CGCTGGATGC TAGCTGGACG TCCCAATCAG 
AACATTAAGG GTGCATGGGT GAGTGGGTTC TTCGACCAGG GCTCTTTTCT GGAGATCATG 
CAGCCATGGG CACAGAGTGT TGTCGTAGGA CGGGCCAGGT TGGGTGGGAT CCCAACTGGA 
GTAGTTGCCG TGGAAACCCG ATCAGTGGAG CTTTCAATCC CAGCAGACCC AGCTAATCTG 
GACTCGGAAG CCAAGATAAT CCAGCAGGCT GGGCAAGTGT GGTTCCCTGA TTCTGCTTTC 
AAAACTGCAC AAGCCATTAA AGACTTCAAT AGAGAGGGAC TTCCGCTTAT AGTGTTTGCC 
AACTGGAGAG GTTTTTCTGG AGGCATGAAA GACATGTATG ACCAGGTGCT GAAGTTTGGA 
GCGTATATAG TGGACGGTCT GAGAGAGTAC AGACAGCCGG TTCTGGTGTA TATCCCACCA 
CAGGCGGAGC TCCGGGGAGG ATCATGGGTT GTCATAGACC CTACTATCAA TCCTCGCCAC 
ATGGAAATGT ATGCAGACAA AGACAGCCGT GGCGGTGTGC TGGAGCCTGA AGGCACAGTG 
GAGATCAAGT TCCGCAAAAA AGACCTGGTG AAGACCATGC GACGGGTGGA CCCTGTGTAC 
ATGGGGCTTG CAGAAAGACT GGGAACCCCA GAGTTGAGTG TGTCTGAGCG TAAAGAGCTG 
GAGAGTAAAC TAAAAGAGAG AGAAGAGTTC CTGCTGCCCA TATATCATCA AGTAGCTGTT 
CAGTTCGCTG ACCTTCATGA CACACCGGGC CGCATGCAGG AGAAAGGAGT CATCACTGAT 
ATCCTTGAGT GGAGCACGTC TCGGCAGTTT TTCTACTGGC GTTTGCGGAG GTTATTGCTG 
GAGGAAACGG TGAAGAGGAA GATCCAGTGT GCTAACAGTG AGCTGACGGA CGGACAGGTT 
CAGGCTATGC TTCGCCGTTG GTTCGTGGAA GCAGAGGGAG CTGTGAAAGC GTATTTGTGG 
GACAGTAATG AAGATGTGGT GGAGTGGTTG GAGAGGCAGC TGGCAGAGGA AGAAGGCGCC 
AGATCCGTCA TCGACGAGAA TATCAAATAT ATTCGCAGAG ATCACATCCT CAAACAGATC 
CGCAGCCTGG TTCAAGCTAA CCCTGAGGTG GCTATGGACT CCATTGTACA CATGACCCAG 
CACATTTCCC CCACACAGCG CGCCGAAGTC GTCCGCATCC TCTCCACCAT GGACACCCCA 
GCCTCCTCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211600.1
CDS	242..7393
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRKLSAPLQDYKPPANTANTSGATEAASDSAESEDRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_017356111.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356111.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGA TTGAGGAATC TGTGCGCTCC ATGGTCATGC GCTACGGCAG TCGCTTGTGG 
AAGCTCCGTG TGCTTCAAGC CGAGCTGAAG ATCAACATCC GATTGACTCC CACCGGCAAA 
CAGATCCCCA TCCGCCTCTT CCTGACCAAC GAGAGTGGCT ACTACCTGGA CATCAGCCTG 
TACAAGGAAG TCACTGACTC CCGTACAGGA CAGATCATGT TCCAGGCATA TGGAGATAAG 
CAGGGTCCTC TTCATGGCAT GCTCATCAAC ACACCCTACG TCACAAAAGA CCTGCTGCAG 
TCCAAACGCT TCCAGGCACA GAGCCTCGGC ACTACCTATG TTTACGACTT CCCAGAAATG 
TTTCGCCAGG CTTTAAGGAA GCTGTGGCAA TCCATGGATG CACACGCCAA CTTGCCCAAG 
TGCCCTCTTC CATCAGAACT GCTCACCTTC ACAGAGCTGG TTCTGGATTC TCAGGGTCAG 
CTGGTGCAGA TGAACCGCCT ACCTGGAGGG AATGAGATTG GCATGGTAGC ATGGCGAATG 
ACCCTCCGGA CCCCTGAGTA TCCAGCCGGC CGTGAGATTA TAGTGATCAG CAATGACATC 
ACACATAAGA TTGGCTCGTT TGGGCCACAA GAAGATGTTC TGTTCCAGCA AGCTTCAGAA 
ATGGCAAGAG AAAGTGGCAT TCCACGGATC TATATTGCTG CCAACAGCGG AGCTCGTATA 
GGCCTGGCAG AGGAGATCAG ACACATGTTT CATGTGGCCT GGCAGGATCC GGTGGACCCA 
TACAAGGGTT TTAAGTACCT ATACCTCACA CCACAGGACT ATAAGAAGGT GTCTGCTCTG 
AACTCAGTCC ACTGTGAACA TGTGGAGGAT GAGGGGGAAT CCAGGTATAA AATCACTGAT 
ATCATTGGGA AAGAGGAAGG GTTGGGTGTG GAGAACTTGA GAGGTTCTGG CATGATCGCA 
GGTGAATCGT CACTGGCGTA TGAGGATATT ATCACCATGA ATCTGGTGAC ATGCAGGGCC 
ATTGGAATCG GAGCGTACTT GGTGAGACTG GGCCAAAGAA CCATACAGGT GGAAAACTCT 
CATATCATCC TGACTGGAGC CGGAGCTCTC AATAAGGTTC TCGGGCGTGA GGTGTACACT 
TCCAATAACC AACTGGGTGG AGTACAGATC ATGCACAATA ACGGAGTCAC ACACACCAAT 
GTGTGCGATG ATTTTGAGGG AGTGTATACA TTGCTGCACT GGCTGTCTTA CATGCCAAAG 
AATATGTCCA GCCCTGTGCC GATACTCTCT GCTAAAGATC CCATTGATCG AGCGATAGAG 
TTTGTCCCAA CCAAGGCACC TTATGACCCA CGCTGGATGC TAGCTGGACG TCCCAATCAG 
AACATTAAGG GTGCATGGGT GAGTGGGTTC TTCGACCAGG GCTCTTTTCT GGAGATCATG 
CAGCCATGGG CACAGAGTGT TGTCGTAGGA CGGGCCAGGT TGGGTGGGAT CCCAACTGGA 
GTAGTTGCCG TGGAAACCCG ATCAGTGGAG CTTTCAATCC CAGCAGACCC AGCTAATCTG 
GACTCGGAAG CCAAGATAAT CCAGCAGGCT GGGCAAGTGT GGTTCCCTGA TTCTGCTTTC 
AAAACTGCAC AAGCCATTAA AGACTTCAAT AGAGAGGGAC TTCCGCTTAT AGTGTTTGCC 
AACTGGAGAG GTTTTTCTGG AGGCATGAAA GACATGTATG ACCAGGTGCT GAAGTTTGGA 
GCGTATATAG TGGACGGTCT GAGAGAGTAC AGACAGCCGG TTCTGGTGTA TATCCCACCA 
CAGGCGGAGC TCCGGGGAGG ATCATGGGTT GTCATAGACC CTACTATCAA TCCTCGCCAC 
ATGGAAATGT ATGCAGACAA AGACAGCCGT GGCGGTGTGC TGGAGCCTGA AGGCACAGTG 
GAGATCAAGT TCCGCAAAAA AGACCTGGTG AAGACCATGC GACGGGTGGA CCCTGTGTAC 
ATGGGGCTTG CAGAAAGACT GGGAACCCCA GAGTTGAGTG TGTCTGAGCG TAAAGAGCTG 
GAGAGTAAAC TAAAAGAGAG AGAAGAGTTC CTGCTGCCCA TATATCATCA AGTAGCTGTT 
CAGTTCGCTG ACCTTCATGA CACACCGGGC CGCATGCAGG AGAAAGGAGT CATCACTGAT 
ATCCTTGAGT GGAGCACGTC TCGGCAGTTT TTCTACTGGC GTTTGCGGAG GTTATTGCTG 
GAGGAAACGG TGAAGAGGAA GATCCAGTGT GCTAACAGTG AGCTGACGGA CGGACAGGTT 
CAGGCTATGC TTCGCCGTTG GTTCGTGGAA GCAGAGGGAG CTGTGAAAGC GTATTTGTGG 
GACAGTAATG AAGATGTGGT GGAGTGGTTG GAGAGGCAGC TGGCAGAGGA AGAAGGCGCC 
AGATCCGTCA TCGACGAGAA TATCAAATAT ATTCGCAGAG ATCACATCCT CAAACAGATC 
CGCAGCCTGG TTCAAGCTAA CCCTGAGGTG GCTATGGACT CCATTGTACA CATGACCCAG 
CACATTTCCC CCACACAGCG CGCCGAAGTC GTCCGCATCC TCTCCACCAT GGACACCCCA 
GCCTCCTCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_017356111.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211600.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X8, mRNA.

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CloneID	ODa59092
Clone ID Related Accession (Same CDS sequence)	XM_017356111.1 , XM_017356111.2
Accession Version	XM_017356111.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356111.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGA TTGAGGAATC TGTGCGCTCC ATGGTCATGC GCTACGGCAG TCGCTTGTGG 
AAGCTCCGTG TGCTTCAAGC CGAGCTGAAG ATCAACATCC GATTGACTCC CACCGGCAAA 
CAGATCCCCA TCCGCCTCTT CCTGACCAAC GAGAGTGGCT ACTACCTGGA CATCAGCCTG 
TACAAGGAAG TCACTGACTC CCGTACAGGA CAGATCATGT TCCAGGCATA TGGAGATAAG 
CAGGGTCCTC TTCATGGCAT GCTCATCAAC ACACCCTACG TCACAAAAGA CCTGCTGCAG 
TCCAAACGCT TCCAGGCACA GAGCCTCGGC ACTACCTATG TTTACGACTT CCCAGAAATG 
TTTCGCCAGG CTTTAAGGAA GCTGTGGCAA TCCATGGATG CACACGCCAA CTTGCCCAAG 
TGCCCTCTTC CATCAGAACT GCTCACCTTC ACAGAGCTGG TTCTGGATTC TCAGGGTCAG 
CTGGTGCAGA TGAACCGCCT ACCTGGAGGG AATGAGATTG GCATGGTAGC ATGGCGAATG 
ACCCTCCGGA CCCCTGAGTA TCCAGCCGGC CGTGAGATTA TAGTGATCAG CAATGACATC 
ACACATAAGA TTGGCTCGTT TGGGCCACAA GAAGATGTTC TGTTCCAGCA AGCTTCAGAA 
ATGGCAAGAG AAAGTGGCAT TCCACGGATC TATATTGCTG CCAACAGCGG AGCTCGTATA 
GGCCTGGCAG AGGAGATCAG ACACATGTTT CATGTGGCCT GGCAGGATCC GGTGGACCCA 
TACAAGGGTT TTAAGTACCT ATACCTCACA CCACAGGACT ATAAGAAGGT GTCTGCTCTG 
AACTCAGTCC ACTGTGAACA TGTGGAGGAT GAGGGGGAAT CCAGGTATAA AATCACTGAT 
ATCATTGGGA AAGAGGAAGG GTTGGGTGTG GAGAACTTGA GAGGTTCTGG CATGATCGCA 
GGTGAATCGT CACTGGCGTA TGAGGATATT ATCACCATGA ATCTGGTGAC ATGCAGGGCC 
ATTGGAATCG GAGCGTACTT GGTGAGACTG GGCCAAAGAA CCATACAGGT GGAAAACTCT 
CATATCATCC TGACTGGAGC CGGAGCTCTC AATAAGGTTC TCGGGCGTGA GGTGTACACT 
TCCAATAACC AACTGGGTGG AGTACAGATC ATGCACAATA ACGGAGTCAC ACACACCAAT 
GTGTGCGATG ATTTTGAGGG AGTGTATACA TTGCTGCACT GGCTGTCTTA CATGCCAAAG 
AATATGTCCA GCCCTGTGCC GATACTCTCT GCTAAAGATC CCATTGATCG AGCGATAGAG 
TTTGTCCCAA CCAAGGCACC TTATGACCCA CGCTGGATGC TAGCTGGACG TCCCAATCAG 
AACATTAAGG GTGCATGGGT GAGTGGGTTC TTCGACCAGG GCTCTTTTCT GGAGATCATG 
CAGCCATGGG CACAGAGTGT TGTCGTAGGA CGGGCCAGGT TGGGTGGGAT CCCAACTGGA 
GTAGTTGCCG TGGAAACCCG ATCAGTGGAG CTTTCAATCC CAGCAGACCC AGCTAATCTG 
GACTCGGAAG CCAAGATAAT CCAGCAGGCT GGGCAAGTGT GGTTCCCTGA TTCTGCTTTC 
AAAACTGCAC AAGCCATTAA AGACTTCAAT AGAGAGGGAC TTCCGCTTAT AGTGTTTGCC 
AACTGGAGAG GTTTTTCTGG AGGCATGAAA GACATGTATG ACCAGGTGCT GAAGTTTGGA 
GCGTATATAG TGGACGGTCT GAGAGAGTAC AGACAGCCGG TTCTGGTGTA TATCCCACCA 
CAGGCGGAGC TCCGGGGAGG ATCATGGGTT GTCATAGACC CTACTATCAA TCCTCGCCAC 
ATGGAAATGT ATGCAGACAA AGACAGCCGT GGCGGTGTGC TGGAGCCTGA AGGCACAGTG 
GAGATCAAGT TCCGCAAAAA AGACCTGGTG AAGACCATGC GACGGGTGGA CCCTGTGTAC 
ATGGGGCTTG CAGAAAGACT GGGAACCCCA GAGTTGAGTG TGTCTGAGCG TAAAGAGCTG 
GAGAGTAAAC TAAAAGAGAG AGAAGAGTTC CTGCTGCCCA TATATCATCA AGTAGCTGTT 
CAGTTCGCTG ACCTTCATGA CACACCGGGC CGCATGCAGG AGAAAGGAGT CATCACTGAT 
ATCCTTGAGT GGAGCACGTC TCGGCAGTTT TTCTACTGGC GTTTGCGGAG GTTATTGCTG 
GAGGAAACGG TGAAGAGGAA GATCCAGTGT GCTAACAGTG AGCTGACGGA CGGACAGGTT 
CAGGCTATGC TTCGCCGTTG GTTCGTGGAA GCAGAGGGAG CTGTGAAAGC GTATTTGTGG 
GACAGTAATG AAGATGTGGT GGAGTGGTTG GAGAGGCAGC TGGCAGAGGA AGAAGGCGCC 
AGATCCGTCA TCGACGAGAA TATCAAATAT ATTCGCAGAG ATCACATCCT CAAACAGATC 
CGCAGCCTGG TTCAAGCTAA CCCTGAGGTG GCTATGGACT CCATTGTACA CATGACCCAG 
CACATTTCCC CCACACAGCG CGCCGAAGTC GTCCGCATCC TCTCCACCAT GGACACCCCA 
GCCTCCTCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211600.1
CDS	237..7388
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRKLSAPLQDYKPPANTANTSGATEAASDSAESEDRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_017356111.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356111.2

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGA TTGAGGAATC TGTGCGCTCC ATGGTCATGC GCTACGGCAG TCGCTTGTGG 
AAGCTCCGTG TGCTTCAAGC CGAGCTGAAG ATCAACATCC GATTGACTCC CACCGGCAAA 
CAGATCCCCA TCCGCCTCTT CCTGACCAAC GAGAGTGGCT ACTACCTGGA CATCAGCCTG 
TACAAGGAAG TCACTGACTC CCGTACAGGA CAGATCATGT TCCAGGCATA TGGAGATAAG 
CAGGGTCCTC TTCATGGCAT GCTCATCAAC ACACCCTACG TCACAAAAGA CCTGCTGCAG 
TCCAAACGCT TCCAGGCACA GAGCCTCGGC ACTACCTATG TTTACGACTT CCCAGAAATG 
TTTCGCCAGG CTTTAAGGAA GCTGTGGCAA TCCATGGATG CACACGCCAA CTTGCCCAAG 
TGCCCTCTTC CATCAGAACT GCTCACCTTC ACAGAGCTGG TTCTGGATTC TCAGGGTCAG 
CTGGTGCAGA TGAACCGCCT ACCTGGAGGG AATGAGATTG GCATGGTAGC ATGGCGAATG 
ACCCTCCGGA CCCCTGAGTA TCCAGCCGGC CGTGAGATTA TAGTGATCAG CAATGACATC 
ACACATAAGA TTGGCTCGTT TGGGCCACAA GAAGATGTTC TGTTCCAGCA AGCTTCAGAA 
ATGGCAAGAG AAAGTGGCAT TCCACGGATC TATATTGCTG CCAACAGCGG AGCTCGTATA 
GGCCTGGCAG AGGAGATCAG ACACATGTTT CATGTGGCCT GGCAGGATCC GGTGGACCCA 
TACAAGGGTT TTAAGTACCT ATACCTCACA CCACAGGACT ATAAGAAGGT GTCTGCTCTG 
AACTCAGTCC ACTGTGAACA TGTGGAGGAT GAGGGGGAAT CCAGGTATAA AATCACTGAT 
ATCATTGGGA AAGAGGAAGG GTTGGGTGTG GAGAACTTGA GAGGTTCTGG CATGATCGCA 
GGTGAATCGT CACTGGCGTA TGAGGATATT ATCACCATGA ATCTGGTGAC ATGCAGGGCC 
ATTGGAATCG GAGCGTACTT GGTGAGACTG GGCCAAAGAA CCATACAGGT GGAAAACTCT 
CATATCATCC TGACTGGAGC CGGAGCTCTC AATAAGGTTC TCGGGCGTGA GGTGTACACT 
TCCAATAACC AACTGGGTGG AGTACAGATC ATGCACAATA ACGGAGTCAC ACACACCAAT 
GTGTGCGATG ATTTTGAGGG AGTGTATACA TTGCTGCACT GGCTGTCTTA CATGCCAAAG 
AATATGTCCA GCCCTGTGCC GATACTCTCT GCTAAAGATC CCATTGATCG AGCGATAGAG 
TTTGTCCCAA CCAAGGCACC TTATGACCCA CGCTGGATGC TAGCTGGACG TCCCAATCAG 
AACATTAAGG GTGCATGGGT GAGTGGGTTC TTCGACCAGG GCTCTTTTCT GGAGATCATG 
CAGCCATGGG CACAGAGTGT TGTCGTAGGA CGGGCCAGGT TGGGTGGGAT CCCAACTGGA 
GTAGTTGCCG TGGAAACCCG ATCAGTGGAG CTTTCAATCC CAGCAGACCC AGCTAATCTG 
GACTCGGAAG CCAAGATAAT CCAGCAGGCT GGGCAAGTGT GGTTCCCTGA TTCTGCTTTC 
AAAACTGCAC AAGCCATTAA AGACTTCAAT AGAGAGGGAC TTCCGCTTAT AGTGTTTGCC 
AACTGGAGAG GTTTTTCTGG AGGCATGAAA GACATGTATG ACCAGGTGCT GAAGTTTGGA 
GCGTATATAG TGGACGGTCT GAGAGAGTAC AGACAGCCGG TTCTGGTGTA TATCCCACCA 
CAGGCGGAGC TCCGGGGAGG ATCATGGGTT GTCATAGACC CTACTATCAA TCCTCGCCAC 
ATGGAAATGT ATGCAGACAA AGACAGCCGT GGCGGTGTGC TGGAGCCTGA AGGCACAGTG 
GAGATCAAGT TCCGCAAAAA AGACCTGGTG AAGACCATGC GACGGGTGGA CCCTGTGTAC 
ATGGGGCTTG CAGAAAGACT GGGAACCCCA GAGTTGAGTG TGTCTGAGCG TAAAGAGCTG 
GAGAGTAAAC TAAAAGAGAG AGAAGAGTTC CTGCTGCCCA TATATCATCA AGTAGCTGTT 
CAGTTCGCTG ACCTTCATGA CACACCGGGC CGCATGCAGG AGAAAGGAGT CATCACTGAT 
ATCCTTGAGT GGAGCACGTC TCGGCAGTTT TTCTACTGGC GTTTGCGGAG GTTATTGCTG 
GAGGAAACGG TGAAGAGGAA GATCCAGTGT GCTAACAGTG AGCTGACGGA CGGACAGGTT 
CAGGCTATGC TTCGCCGTTG GTTCGTGGAA GCAGAGGGAG CTGTGAAAGC GTATTTGTGG 
GACAGTAATG AAGATGTGGT GGAGTGGTTG GAGAGGCAGC TGGCAGAGGA AGAAGGCGCC 
AGATCCGTCA TCGACGAGAA TATCAAATAT ATTCGCAGAG ATCACATCCT CAAACAGATC 
CGCAGCCTGG TTCAAGCTAA CCCTGAGGTG GCTATGGACT CCATTGTACA CATGACCCAG 
CACATTTCCC CCACACAGCG CGCCGAAGTC GTCCGCATCC TCTCCACCAT GGACACCCCA 
GCCTCCTCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_017356112.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211601.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X10, mRNA.

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CloneID	ODa59093
Clone ID Related Accession (Same CDS sequence)	XM_017356112.2 , XM_017356112.1
Accession Version	XM_017356112.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356112.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG AGAGAATTCC CCAAATTTTT TACATTCCGG 
GCTCGAGACA AGTTTGAGGA AGACCGTATC TACAGACACT TGGAACCTGC ATTAGCTTTC 
CAGCTGGAGC TCAACCGCAT GCGTAATTTT GCACTGACAG CCATCCCCTG TGCCAATCAT 
AAGATGCACC TGTACTTGGG AGCTGCCCGT GTGGAAGTAG GCACTGAGGT CACAGATTAC 
CGCTTCTTTG TGCGGGCAAT CATCCGTCAC TCAGATTTGG TCACCAAGGA AGCATCATTT 
GAGTACCTGC ACAACGAGGC AGAACGCTTG CTGCTGGAGG CCATGGATGA ACTGGAAGTG 
GCTTTCAACA ACACCACTGT GCGAACCGAC TGCAATCACA TCTTCCTCAA CTTCGTTCCA 
ACAGTCATCA TGGACCCTTC AAAGATTGAG GAATCTGTGC GCTCCATGGT CATGCGCTAC 
GGCAGTCGCT TGTGGAAGCT CCGTGTGCTT CAAGCCGAGC TGAAGATCAA CATCCGATTG 
ACTCCCACCG GCAAACAGAT CCCCATCCGC CTCTTCCTGA CCAACGAGAG TGGCTACTAC 
CTGGACATCA GCCTGTACAA GGAAGTCACT GACTCCCGTA CAGGACAGGT GGGGCACAAA 
GACCGACAGA TCATGTTCCA GGCATATGGA GATAAGCAGG GTCCTCTTCA TGGCATGCTC 
ATCAACACAC CCTACGTCAC AAAAGACCTG CTGCAGTCCA AACGCTTCCA GGCACAGAGC 
CTCGGCACTA CCTATGTTTA CGACTTCCCA GAAATGTTTC GCCAGGCTTT AAGGAAGCTG 
TGGCAATCCA TGGATGCACA CGCCAACTTG CCCAAGTGCC CTCTTCCATC AGAACTGCTC 
ACCTTCACAG AGCTGGTTCT GGATTCTCAG GGTCAGCTGG TGCAGATGAA CCGCCTACCT 
GGAGGGAATG AGATTGGCAT GGTAGCATGG CGAATGACCC TCCGGACCCC TGAGTATCCA 
GCCGGCCGTG AGATTATAGT GATCAGCAAT GACATCACAC ATAAGATTGG CTCGTTTGGG 
CCACAAGAAG ATGTTCTGTT CCAGCAAGCT TCAGAAATGG CAAGAGAAAG TGGCATTCCA 
CGGATCTATA TTGCTGCCAA CAGCGGAGCT CGTATAGGCC TGGCAGAGGA GATCAGACAC 
ATGTTTCATG TGGCCTGGCA GGATCCGGTG GACCCATACA AGGGTTTTAA GTACCTATAC 
CTCACACCAC AGGACTATAA GAAGGTGTCT GCTCTGAACT CAGTCCACTG TGAACATGTG 
GAGGATGAGG GGGAATCCAG GTATAAAATC ACTGATATCA TTGGGAAAGA GGAAGGGTTG 
GGTGTGGAGA ACTTGAGAGG TTCTGGCATG ATCGCAGGTG AATCGTCACT GGCGTATGAG 
GATATTATCA CCATGAATCT GGTGACATGC AGGGCCATTG GAATCGGAGC GTACTTGGTG 
AGACTGGGCC AAAGAACCAT ACAGGTGGAA AACTCTCATA TCATCCTGAC TGGAGCCGGA 
GCTCTCAATA AGGTTCTCGG GCGTGAGGTG TACACTTCCA ATAACCAACT GGGTGGAGTA 
CAGATCATGC ACAATAACGG AGTCACACAC ACCAATGTGT GCGATGATTT TGAGGGAGTG 
TATACATTGC TGCACTGGCT GTCTTACATG CCAAAGAATA TGTCCAGCCC TGTGCCGATA 
CTCTCTGCTA AAGATCCCAT TGATCGAGCG ATAGAGTTTG TCCCAACCAA GGCACCTTAT 
GACCCACGCT GGATGCTAGC TGGACGTCCC AATCAGAACA TTAAGGGTGC ATGGGTGAGT 
GGGTTCTTCG ACCAGGGCTC TTTTCTGGAG ATCATGCAGC CATGGGCACA GAGTGTTGTC 
GTAGGACGGG CCAGGTTGGG TGGGATCCCA ACTGGAGTAG TTGCCGTGGA AACCCGATCA 
GTGGAGCTTT CAATCCCAGC AGACCCAGCT AATCTGGACT CGGAAGCCAA GATAATCCAG 
CAGGCTGGGC AAGTGTGGTT CCCTGATTCT GCTTTCAAAA CTGCACAAGC CATTAAAGAC 
TTCAATAGAG AGGGACTTCC GCTTATAGTG TTTGCCAACT GGAGAGGTTT TTCTGGAGGC 
ATGAAAGACA TGTATGACCA GGTGCTGAAG TTTGGAGCGT ATATAGTGGA CGGTCTGAGA 
GAGTACAGAC AGCCGGTTCT GGTGTATATC CCACCACAGG CGGAGCTCCG GGGAGGATCA 
TGGGTTGTCA TAGACCCTAC TATCAATCCT CGCCACATGG AAATGTATGC AGACAAAGAC 
AGCCGTGGCG GTGTGCTGGA GCCTGAAGGC ACAGTGGAGA TCAAGTTCCG CAAAAAAGAC 
CTGGTGAAGA CCATGCGACG GGTGGACCCT GTGTACATGG GGCTTGCAGA AAGACTGGGA 
ACCCCAGAGT TGAGTGTGTC TGAGCGTAAA GAGCTGGAGA GTAAACTAAA AGAGAGAGAA 
GAGTTCCTGC TGCCCATATA TCATCAAGTA GCTGTTCAGT TCGCTGACCT TCATGACACA 
CCGGGCCGCA TGCAGGAGAA AGGAGTCATC ACTGATATCC TTGAGTGGAG CACGTCTCGG 
CAGTTTTTCT ACTGGCGTTT GCGGAGGTTA TTGCTGGAGG AAACGGTGAA GAGGAAGATC 
CAGTGTGCTA ACAGTGAGCT GACGGACGGA CAGGTTCAGG CTATGCTTCG CCGTTGGTTC 
GTGGAAGCAG AGGGAGCTGT GAAAGCGTAT TTGTGGGACA GTAATGAAGA TGTGGTGGAG 
TGGTTGGAGA GGCAGCTGGC AGAGGAAGAA GGCGCCAGAT CCGTCATCGA CGAGAATATC 
AAATATATTC GCAGAGATCA CATCCTCAAA CAGATCCGCA GCCTGGTTCA AGCTAACCCT 
GAGGTGGCTA TGGACTCCAT TGTACACATG ACCCAGCACA TTTCCCCCAC ACAGCGCGCC 
GAAGTCGTCC GCATCCTCTC CACCATGGAC ACCCCAGCCT CCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211601.1
CDS	237..7364
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRKLSAPLQDYKPPANTANTSGATEAASDSAESEDRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_017356112.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356112.2

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG AGAGAATTCC CCAAATTTTT TACATTCCGG 
GCTCGAGACA AGTTTGAGGA AGACCGTATC TACAGACACT TGGAACCTGC ATTAGCTTTC 
CAGCTGGAGC TCAACCGCAT GCGTAATTTT GCACTGACAG CCATCCCCTG TGCCAATCAT 
AAGATGCACC TGTACTTGGG AGCTGCCCGT GTGGAAGTAG GCACTGAGGT CACAGATTAC 
CGCTTCTTTG TGCGGGCAAT CATCCGTCAC TCAGATTTGG TCACCAAGGA AGCATCATTT 
GAGTACCTGC ACAACGAGGC AGAACGCTTG CTGCTGGAGG CCATGGATGA ACTGGAAGTG 
GCTTTCAACA ACACCACTGT GCGAACCGAC TGCAATCACA TCTTCCTCAA CTTCGTTCCA 
ACAGTCATCA TGGACCCTTC AAAGATTGAG GAATCTGTGC GCTCCATGGT CATGCGCTAC 
GGCAGTCGCT TGTGGAAGCT CCGTGTGCTT CAAGCCGAGC TGAAGATCAA CATCCGATTG 
ACTCCCACCG GCAAACAGAT CCCCATCCGC CTCTTCCTGA CCAACGAGAG TGGCTACTAC 
CTGGACATCA GCCTGTACAA GGAAGTCACT GACTCCCGTA CAGGACAGGT GGGGCACAAA 
GACCGACAGA TCATGTTCCA GGCATATGGA GATAAGCAGG GTCCTCTTCA TGGCATGCTC 
ATCAACACAC CCTACGTCAC AAAAGACCTG CTGCAGTCCA AACGCTTCCA GGCACAGAGC 
CTCGGCACTA CCTATGTTTA CGACTTCCCA GAAATGTTTC GCCAGGCTTT AAGGAAGCTG 
TGGCAATCCA TGGATGCACA CGCCAACTTG CCCAAGTGCC CTCTTCCATC AGAACTGCTC 
ACCTTCACAG AGCTGGTTCT GGATTCTCAG GGTCAGCTGG TGCAGATGAA CCGCCTACCT 
GGAGGGAATG AGATTGGCAT GGTAGCATGG CGAATGACCC TCCGGACCCC TGAGTATCCA 
GCCGGCCGTG AGATTATAGT GATCAGCAAT GACATCACAC ATAAGATTGG CTCGTTTGGG 
CCACAAGAAG ATGTTCTGTT CCAGCAAGCT TCAGAAATGG CAAGAGAAAG TGGCATTCCA 
CGGATCTATA TTGCTGCCAA CAGCGGAGCT CGTATAGGCC TGGCAGAGGA GATCAGACAC 
ATGTTTCATG TGGCCTGGCA GGATCCGGTG GACCCATACA AGGGTTTTAA GTACCTATAC 
CTCACACCAC AGGACTATAA GAAGGTGTCT GCTCTGAACT CAGTCCACTG TGAACATGTG 
GAGGATGAGG GGGAATCCAG GTATAAAATC ACTGATATCA TTGGGAAAGA GGAAGGGTTG 
GGTGTGGAGA ACTTGAGAGG TTCTGGCATG ATCGCAGGTG AATCGTCACT GGCGTATGAG 
GATATTATCA CCATGAATCT GGTGACATGC AGGGCCATTG GAATCGGAGC GTACTTGGTG 
AGACTGGGCC AAAGAACCAT ACAGGTGGAA AACTCTCATA TCATCCTGAC TGGAGCCGGA 
GCTCTCAATA AGGTTCTCGG GCGTGAGGTG TACACTTCCA ATAACCAACT GGGTGGAGTA 
CAGATCATGC ACAATAACGG AGTCACACAC ACCAATGTGT GCGATGATTT TGAGGGAGTG 
TATACATTGC TGCACTGGCT GTCTTACATG CCAAAGAATA TGTCCAGCCC TGTGCCGATA 
CTCTCTGCTA AAGATCCCAT TGATCGAGCG ATAGAGTTTG TCCCAACCAA GGCACCTTAT 
GACCCACGCT GGATGCTAGC TGGACGTCCC AATCAGAACA TTAAGGGTGC ATGGGTGAGT 
GGGTTCTTCG ACCAGGGCTC TTTTCTGGAG ATCATGCAGC CATGGGCACA GAGTGTTGTC 
GTAGGACGGG CCAGGTTGGG TGGGATCCCA ACTGGAGTAG TTGCCGTGGA AACCCGATCA 
GTGGAGCTTT CAATCCCAGC AGACCCAGCT AATCTGGACT CGGAAGCCAA GATAATCCAG 
CAGGCTGGGC AAGTGTGGTT CCCTGATTCT GCTTTCAAAA CTGCACAAGC CATTAAAGAC 
TTCAATAGAG AGGGACTTCC GCTTATAGTG TTTGCCAACT GGAGAGGTTT TTCTGGAGGC 
ATGAAAGACA TGTATGACCA GGTGCTGAAG TTTGGAGCGT ATATAGTGGA CGGTCTGAGA 
GAGTACAGAC AGCCGGTTCT GGTGTATATC CCACCACAGG CGGAGCTCCG GGGAGGATCA 
TGGGTTGTCA TAGACCCTAC TATCAATCCT CGCCACATGG AAATGTATGC AGACAAAGAC 
AGCCGTGGCG GTGTGCTGGA GCCTGAAGGC ACAGTGGAGA TCAAGTTCCG CAAAAAAGAC 
CTGGTGAAGA CCATGCGACG GGTGGACCCT GTGTACATGG GGCTTGCAGA AAGACTGGGA 
ACCCCAGAGT TGAGTGTGTC TGAGCGTAAA GAGCTGGAGA GTAAACTAAA AGAGAGAGAA 
GAGTTCCTGC TGCCCATATA TCATCAAGTA GCTGTTCAGT TCGCTGACCT TCATGACACA 
CCGGGCCGCA TGCAGGAGAA AGGAGTCATC ACTGATATCC TTGAGTGGAG CACGTCTCGG 
CAGTTTTTCT ACTGGCGTTT GCGGAGGTTA TTGCTGGAGG AAACGGTGAA GAGGAAGATC 
CAGTGTGCTA ACAGTGAGCT GACGGACGGA CAGGTTCAGG CTATGCTTCG CCGTTGGTTC 
GTGGAAGCAG AGGGAGCTGT GAAAGCGTAT TTGTGGGACA GTAATGAAGA TGTGGTGGAG 
TGGTTGGAGA GGCAGCTGGC AGAGGAAGAA GGCGCCAGAT CCGTCATCGA CGAGAATATC 
AAATATATTC GCAGAGATCA CATCCTCAAA CAGATCCGCA GCCTGGTTCA AGCTAACCCT 
GAGGTGGCTA TGGACTCCAT TGTACACATG ACCCAGCACA TTTCCCCCAC ACAGCGCGCC 
GAAGTCGTCC GCATCCTCTC CACCATGGAC ACCCCAGCCT CCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_017356112.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211601.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X4, mRNA.

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CloneID	ODa59093
Clone ID Related Accession (Same CDS sequence)	XM_017356112.2 , XM_017356112.1
Accession Version	XM_017356112.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG AGAGAATTCC CCAAATTTTT TACATTCCGG 
GCTCGAGACA AGTTTGAGGA AGACCGTATC TACAGACACT TGGAACCTGC ATTAGCTTTC 
CAGCTGGAGC TCAACCGCAT GCGTAATTTT GCACTGACAG CCATCCCCTG TGCCAATCAT 
AAGATGCACC TGTACTTGGG AGCTGCCCGT GTGGAAGTAG GCACTGAGGT CACAGATTAC 
CGCTTCTTTG TGCGGGCAAT CATCCGTCAC TCAGATTTGG TCACCAAGGA AGCATCATTT 
GAGTACCTGC ACAACGAGGC AGAACGCTTG CTGCTGGAGG CCATGGATGA ACTGGAAGTG 
GCTTTCAACA ACACCACTGT GCGAACCGAC TGCAATCACA TCTTCCTCAA CTTCGTTCCA 
ACAGTCATCA TGGACCCTTC AAAGATTGAG GAATCTGTGC GCTCCATGGT CATGCGCTAC 
GGCAGTCGCT TGTGGAAGCT CCGTGTGCTT CAAGCCGAGC TGAAGATCAA CATCCGATTG 
ACTCCCACCG GCAAACAGAT CCCCATCCGC CTCTTCCTGA CCAACGAGAG TGGCTACTAC 
CTGGACATCA GCCTGTACAA GGAAGTCACT GACTCCCGTA CAGGACAGGT GGGGCACAAA 
GACCGACAGA TCATGTTCCA GGCATATGGA GATAAGCAGG GTCCTCTTCA TGGCATGCTC 
ATCAACACAC CCTACGTCAC AAAAGACCTG CTGCAGTCCA AACGCTTCCA GGCACAGAGC 
CTCGGCACTA CCTATGTTTA CGACTTCCCA GAAATGTTTC GCCAGGCTTT AAGGAAGCTG 
TGGCAATCCA TGGATGCACA CGCCAACTTG CCCAAGTGCC CTCTTCCATC AGAACTGCTC 
ACCTTCACAG AGCTGGTTCT GGATTCTCAG GGTCAGCTGG TGCAGATGAA CCGCCTACCT 
GGAGGGAATG AGATTGGCAT GGTAGCATGG CGAATGACCC TCCGGACCCC TGAGTATCCA 
GCCGGCCGTG AGATTATAGT GATCAGCAAT GACATCACAC ATAAGATTGG CTCGTTTGGG 
CCACAAGAAG ATGTTCTGTT CCAGCAAGCT TCAGAAATGG CAAGAGAAAG TGGCATTCCA 
CGGATCTATA TTGCTGCCAA CAGCGGAGCT CGTATAGGCC TGGCAGAGGA GATCAGACAC 
ATGTTTCATG TGGCCTGGCA GGATCCGGTG GACCCATACA AGGGTTTTAA GTACCTATAC 
CTCACACCAC AGGACTATAA GAAGGTGTCT GCTCTGAACT CAGTCCACTG TGAACATGTG 
GAGGATGAGG GGGAATCCAG GTATAAAATC ACTGATATCA TTGGGAAAGA GGAAGGGTTG 
GGTGTGGAGA ACTTGAGAGG TTCTGGCATG ATCGCAGGTG AATCGTCACT GGCGTATGAG 
GATATTATCA CCATGAATCT GGTGACATGC AGGGCCATTG GAATCGGAGC GTACTTGGTG 
AGACTGGGCC AAAGAACCAT ACAGGTGGAA AACTCTCATA TCATCCTGAC TGGAGCCGGA 
GCTCTCAATA AGGTTCTCGG GCGTGAGGTG TACACTTCCA ATAACCAACT GGGTGGAGTA 
CAGATCATGC ACAATAACGG AGTCACACAC ACCAATGTGT GCGATGATTT TGAGGGAGTG 
TATACATTGC TGCACTGGCT GTCTTACATG CCAAAGAATA TGTCCAGCCC TGTGCCGATA 
CTCTCTGCTA AAGATCCCAT TGATCGAGCG ATAGAGTTTG TCCCAACCAA GGCACCTTAT 
GACCCACGCT GGATGCTAGC TGGACGTCCC AATCAGAACA TTAAGGGTGC ATGGGTGAGT 
GGGTTCTTCG ACCAGGGCTC TTTTCTGGAG ATCATGCAGC CATGGGCACA GAGTGTTGTC 
GTAGGACGGG CCAGGTTGGG TGGGATCCCA ACTGGAGTAG TTGCCGTGGA AACCCGATCA 
GTGGAGCTTT CAATCCCAGC AGACCCAGCT AATCTGGACT CGGAAGCCAA GATAATCCAG 
CAGGCTGGGC AAGTGTGGTT CCCTGATTCT GCTTTCAAAA CTGCACAAGC CATTAAAGAC 
TTCAATAGAG AGGGACTTCC GCTTATAGTG TTTGCCAACT GGAGAGGTTT TTCTGGAGGC 
ATGAAAGACA TGTATGACCA GGTGCTGAAG TTTGGAGCGT ATATAGTGGA CGGTCTGAGA 
GAGTACAGAC AGCCGGTTCT GGTGTATATC CCACCACAGG CGGAGCTCCG GGGAGGATCA 
TGGGTTGTCA TAGACCCTAC TATCAATCCT CGCCACATGG AAATGTATGC AGACAAAGAC 
AGCCGTGGCG GTGTGCTGGA GCCTGAAGGC ACAGTGGAGA TCAAGTTCCG CAAAAAAGAC 
CTGGTGAAGA CCATGCGACG GGTGGACCCT GTGTACATGG GGCTTGCAGA AAGACTGGGA 
ACCCCAGAGT TGAGTGTGTC TGAGCGTAAA GAGCTGGAGA GTAAACTAAA AGAGAGAGAA 
GAGTTCCTGC TGCCCATATA TCATCAAGTA GCTGTTCAGT TCGCTGACCT TCATGACACA 
CCGGGCCGCA TGCAGGAGAA AGGAGTCATC ACTGATATCC TTGAGTGGAG CACGTCTCGG 
CAGTTTTTCT ACTGGCGTTT GCGGAGGTTA TTGCTGGAGG AAACGGTGAA GAGGAAGATC 
CAGTGTGCTA ACAGTGAGCT GACGGACGGA CAGGTTCAGG CTATGCTTCG CCGTTGGTTC 
GTGGAAGCAG AGGGAGCTGT GAAAGCGTAT TTGTGGGACA GTAATGAAGA TGTGGTGGAG 
TGGTTGGAGA GGCAGCTGGC AGAGGAAGAA GGCGCCAGAT CCGTCATCGA CGAGAATATC 
AAATATATTC GCAGAGATCA CATCCTCAAA CAGATCCGCA GCCTGGTTCA AGCTAACCCT 
GAGGTGGCTA TGGACTCCAT TGTACACATG ACCCAGCACA TTTCCCCCAC ACAGCGCGCC 
GAAGTCGTCC GCATCCTCTC CACCATGGAC ACCCCAGCCT CCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211601.1
CDS	242..7369
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRKLSAPLQDYKPPANTANTSGATEAASDSAESEDRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_017356112.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356112.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG AGAGAATTCC CCAAATTTTT TACATTCCGG 
GCTCGAGACA AGTTTGAGGA AGACCGTATC TACAGACACT TGGAACCTGC ATTAGCTTTC 
CAGCTGGAGC TCAACCGCAT GCGTAATTTT GCACTGACAG CCATCCCCTG TGCCAATCAT 
AAGATGCACC TGTACTTGGG AGCTGCCCGT GTGGAAGTAG GCACTGAGGT CACAGATTAC 
CGCTTCTTTG TGCGGGCAAT CATCCGTCAC TCAGATTTGG TCACCAAGGA AGCATCATTT 
GAGTACCTGC ACAACGAGGC AGAACGCTTG CTGCTGGAGG CCATGGATGA ACTGGAAGTG 
GCTTTCAACA ACACCACTGT GCGAACCGAC TGCAATCACA TCTTCCTCAA CTTCGTTCCA 
ACAGTCATCA TGGACCCTTC AAAGATTGAG GAATCTGTGC GCTCCATGGT CATGCGCTAC 
GGCAGTCGCT TGTGGAAGCT CCGTGTGCTT CAAGCCGAGC TGAAGATCAA CATCCGATTG 
ACTCCCACCG GCAAACAGAT CCCCATCCGC CTCTTCCTGA CCAACGAGAG TGGCTACTAC 
CTGGACATCA GCCTGTACAA GGAAGTCACT GACTCCCGTA CAGGACAGGT GGGGCACAAA 
GACCGACAGA TCATGTTCCA GGCATATGGA GATAAGCAGG GTCCTCTTCA TGGCATGCTC 
ATCAACACAC CCTACGTCAC AAAAGACCTG CTGCAGTCCA AACGCTTCCA GGCACAGAGC 
CTCGGCACTA CCTATGTTTA CGACTTCCCA GAAATGTTTC GCCAGGCTTT AAGGAAGCTG 
TGGCAATCCA TGGATGCACA CGCCAACTTG CCCAAGTGCC CTCTTCCATC AGAACTGCTC 
ACCTTCACAG AGCTGGTTCT GGATTCTCAG GGTCAGCTGG TGCAGATGAA CCGCCTACCT 
GGAGGGAATG AGATTGGCAT GGTAGCATGG CGAATGACCC TCCGGACCCC TGAGTATCCA 
GCCGGCCGTG AGATTATAGT GATCAGCAAT GACATCACAC ATAAGATTGG CTCGTTTGGG 
CCACAAGAAG ATGTTCTGTT CCAGCAAGCT TCAGAAATGG CAAGAGAAAG TGGCATTCCA 
CGGATCTATA TTGCTGCCAA CAGCGGAGCT CGTATAGGCC TGGCAGAGGA GATCAGACAC 
ATGTTTCATG TGGCCTGGCA GGATCCGGTG GACCCATACA AGGGTTTTAA GTACCTATAC 
CTCACACCAC AGGACTATAA GAAGGTGTCT GCTCTGAACT CAGTCCACTG TGAACATGTG 
GAGGATGAGG GGGAATCCAG GTATAAAATC ACTGATATCA TTGGGAAAGA GGAAGGGTTG 
GGTGTGGAGA ACTTGAGAGG TTCTGGCATG ATCGCAGGTG AATCGTCACT GGCGTATGAG 
GATATTATCA CCATGAATCT GGTGACATGC AGGGCCATTG GAATCGGAGC GTACTTGGTG 
AGACTGGGCC AAAGAACCAT ACAGGTGGAA AACTCTCATA TCATCCTGAC TGGAGCCGGA 
GCTCTCAATA AGGTTCTCGG GCGTGAGGTG TACACTTCCA ATAACCAACT GGGTGGAGTA 
CAGATCATGC ACAATAACGG AGTCACACAC ACCAATGTGT GCGATGATTT TGAGGGAGTG 
TATACATTGC TGCACTGGCT GTCTTACATG CCAAAGAATA TGTCCAGCCC TGTGCCGATA 
CTCTCTGCTA AAGATCCCAT TGATCGAGCG ATAGAGTTTG TCCCAACCAA GGCACCTTAT 
GACCCACGCT GGATGCTAGC TGGACGTCCC AATCAGAACA TTAAGGGTGC ATGGGTGAGT 
GGGTTCTTCG ACCAGGGCTC TTTTCTGGAG ATCATGCAGC CATGGGCACA GAGTGTTGTC 
GTAGGACGGG CCAGGTTGGG TGGGATCCCA ACTGGAGTAG TTGCCGTGGA AACCCGATCA 
GTGGAGCTTT CAATCCCAGC AGACCCAGCT AATCTGGACT CGGAAGCCAA GATAATCCAG 
CAGGCTGGGC AAGTGTGGTT CCCTGATTCT GCTTTCAAAA CTGCACAAGC CATTAAAGAC 
TTCAATAGAG AGGGACTTCC GCTTATAGTG TTTGCCAACT GGAGAGGTTT TTCTGGAGGC 
ATGAAAGACA TGTATGACCA GGTGCTGAAG TTTGGAGCGT ATATAGTGGA CGGTCTGAGA 
GAGTACAGAC AGCCGGTTCT GGTGTATATC CCACCACAGG CGGAGCTCCG GGGAGGATCA 
TGGGTTGTCA TAGACCCTAC TATCAATCCT CGCCACATGG AAATGTATGC AGACAAAGAC 
AGCCGTGGCG GTGTGCTGGA GCCTGAAGGC ACAGTGGAGA TCAAGTTCCG CAAAAAAGAC 
CTGGTGAAGA CCATGCGACG GGTGGACCCT GTGTACATGG GGCTTGCAGA AAGACTGGGA 
ACCCCAGAGT TGAGTGTGTC TGAGCGTAAA GAGCTGGAGA GTAAACTAAA AGAGAGAGAA 
GAGTTCCTGC TGCCCATATA TCATCAAGTA GCTGTTCAGT TCGCTGACCT TCATGACACA 
CCGGGCCGCA TGCAGGAGAA AGGAGTCATC ACTGATATCC TTGAGTGGAG CACGTCTCGG 
CAGTTTTTCT ACTGGCGTTT GCGGAGGTTA TTGCTGGAGG AAACGGTGAA GAGGAAGATC 
CAGTGTGCTA ACAGTGAGCT GACGGACGGA CAGGTTCAGG CTATGCTTCG CCGTTGGTTC 
GTGGAAGCAG AGGGAGCTGT GAAAGCGTAT TTGTGGGACA GTAATGAAGA TGTGGTGGAG 
TGGTTGGAGA GGCAGCTGGC AGAGGAAGAA GGCGCCAGAT CCGTCATCGA CGAGAATATC 
AAATATATTC GCAGAGATCA CATCCTCAAA CAGATCCGCA GCCTGGTTCA AGCTAACCCT 
GAGGTGGCTA TGGACTCCAT TGTACACATG ACCCAGCACA TTTCCCCCAC ACAGCGCGCC 
GAAGTCGTCC GCATCCTCTC CACCATGGAC ACCCCAGCCT CCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_017356113.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211602.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X5, mRNA.

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CloneID	ODa59094
Clone ID Related Accession (Same CDS sequence)	XM_017356113.1 , XM_017356113.2
Accession Version	XM_017356113.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG AGAGAATTCC CCAAATTTTT TACATTCCGG 
GCTCGAGACA AGTTTGAGGA AGACCGTATC TACAGACACT TGGAACCTGC ATTAGCTTTC 
CAGCTGGAGC TCAACCGCAT GCGTAATTTT GCACTGACAG CCATCCCCTG TGCCAATCAT 
AAGATGCACC TGTACTTGGG AGCTGCCCGT GTGGAAGTAG GCACTGAGGT CACAGATTAC 
CGCTTCTTTG TGCGGGCAAT CATCCGTCAC TCAGATTTGG TCACCAAGGA AGCATCATTT 
GAGTACCTGC ACAACGAGGC AGAACGCTTG CTGCTGGAGG CCATGGATGA ACTGGAAGTG 
GCTTTCAACA ACACCACTGT GCGAACCGAC TGCAATCACA TCTTCCTCAA CTTCGTTCCA 
ACAGTCATCA TGGACCCTTC AAAGATTGAG GAATCTGTGC GCTCCATGGT CATGCGCTAC 
GGCAGTCGCT TGTGGAAGCT CCGTGTGCTT CAAGCCGAGC TGAAGATCAA CATCCGATTG 
ACTCCCACCG GCAAACAGAT CCCCATCCGC CTCTTCCTGA CCAACGAGAG TGGCTACTAC 
CTGGACATCA GCCTGTACAA GGAAGTCACT GACTCCCGTA CAGGACAGAT CATGTTCCAG 
GCATATGGAG ATAAGCAGGG TCCTCTTCAT GGCATGCTCA TCAACACACC CTACGTCACA 
AAAGACCTGC TGCAGTCCAA ACGCTTCCAG GCACAGAGCC TCGGCACTAC CTATGTTTAC 
GACTTCCCAG AAATGTTTCG CCAGGCTTTA AGGAAGCTGT GGCAATCCAT GGATGCACAC 
GCCAACTTGC CCAAGTGCCC TCTTCCATCA GAACTGCTCA CCTTCACAGA GCTGGTTCTG 
GATTCTCAGG GTCAGCTGGT GCAGATGAAC CGCCTACCTG GAGGGAATGA GATTGGCATG 
GTAGCATGGC GAATGACCCT CCGGACCCCT GAGTATCCAG CCGGCCGTGA GATTATAGTG 
ATCAGCAATG ACATCACACA TAAGATTGGC TCGTTTGGGC CACAAGAAGA TGTTCTGTTC 
CAGCAAGCTT CAGAAATGGC AAGAGAAAGT GGCATTCCAC GGATCTATAT TGCTGCCAAC 
AGCGGAGCTC GTATAGGCCT GGCAGAGGAG ATCAGACACA TGTTTCATGT GGCCTGGCAG 
GATCCGGTGG ACCCATACAA GGGTTTTAAG TACCTATACC TCACACCACA GGACTATAAG 
AAGGTGTCTG CTCTGAACTC AGTCCACTGT GAACATGTGG AGGATGAGGG GGAATCCAGG 
TATAAAATCA CTGATATCAT TGGGAAAGAG GAAGGGTTGG GTGTGGAGAA CTTGAGAGGT 
TCTGGCATGA TCGCAGGTGA ATCGTCACTG GCGTATGAGG ATATTATCAC CATGAATCTG 
GTGACATGCA GGGCCATTGG AATCGGAGCG TACTTGGTGA GACTGGGCCA AAGAACCATA 
CAGGTGGAAA ACTCTCATAT CATCCTGACT GGAGCCGGAG CTCTCAATAA GGTTCTCGGG 
CGTGAGGTGT ACACTTCCAA TAACCAACTG GGTGGAGTAC AGATCATGCA CAATAACGGA 
GTCACACACA CCAATGTGTG CGATGATTTT GAGGGAGTGT ATACATTGCT GCACTGGCTG 
TCTTACATGC CAAAGAATAT GTCCAGCCCT GTGCCGATAC TCTCTGCTAA AGATCCCATT 
GATCGAGCGA TAGAGTTTGT CCCAACCAAG GCACCTTATG ACCCACGCTG GATGCTAGCT 
GGACGTCCCA ATCAGAACAT TAAGGGTGCA TGGGTGAGTG GGTTCTTCGA CCAGGGCTCT 
TTTCTGGAGA TCATGCAGCC ATGGGCACAG AGTGTTGTCG TAGGACGGGC CAGGTTGGGT 
GGGATCCCAA CTGGAGTAGT TGCCGTGGAA ACCCGATCAG TGGAGCTTTC AATCCCAGCA 
GACCCAGCTA ATCTGGACTC GGAAGCCAAG ATAATCCAGC AGGCTGGGCA AGTGTGGTTC 
CCTGATTCTG CTTTCAAAAC TGCACAAGCC ATTAAAGACT TCAATAGAGA GGGACTTCCG 
CTTATAGTGT TTGCCAACTG GAGAGGTTTT TCTGGAGGCA TGAAAGACAT GTATGACCAG 
GTGCTGAAGT TTGGAGCGTA TATAGTGGAC GGTCTGAGAG AGTACAGACA GCCGGTTCTG 
GTGTATATCC CACCACAGGC GGAGCTCCGG GGAGGATCAT GGGTTGTCAT AGACCCTACT 
ATCAATCCTC GCCACATGGA AATGTATGCA GACAAAGACA GCCGTGGCGG TGTGCTGGAG 
CCTGAAGGCA CAGTGGAGAT CAAGTTCCGC AAAAAAGACC TGGTGAAGAC CATGCGACGG 
GTGGACCCTG TGTACATGGG GCTTGCAGAA AGACTGGGAA CCCCAGAGTT GAGTGTGTCT 
GAGCGTAAAG AGCTGGAGAG TAAACTAAAA GAGAGAGAAG AGTTCCTGCT GCCCATATAT 
CATCAAGTAG CTGTTCAGTT CGCTGACCTT CATGACACAC CGGGCCGCAT GCAGGAGAAA 
GGAGTCATCA CTGATATCCT TGAGTGGAGC ACGTCTCGGC AGTTTTTCTA CTGGCGTTTG 
CGGAGGTTAT TGCTGGAGGA AACGGTGAAG AGGAAGATCC AGTGTGCTAA CAGTGAGCTG 
ACGGACGGAC AGGTTCAGGC TATGCTTCGC CGTTGGTTCG TGGAAGCAGA GGGAGCTGTG 
AAAGCGTATT TGTGGGACAG TAATGAAGAT GTGGTGGAGT GGTTGGAGAG GCAGCTGGCA 
GAGGAAGAAG GCGCCAGATC CGTCATCGAC GAGAATATCA AATATATTCG CAGAGATCAC 
ATCCTCAAAC AGATCCGCAG CCTGGTTCAA GCTAACCCTG AGGTGGCTAT GGACTCCATT 
GTACACATGA CCCAGCACAT TTCCCCCACA CAGCGCGCCG AAGTCGTCCG CATCCTCTCC 
ACCATGGACA CCCCAGCCTC CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211602.1
CDS	242..7348
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRKLSAPLQDYKPPANTANTSGATEAASDSAESEDRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_017356113.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356113.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG AGAGAATTCC CCAAATTTTT TACATTCCGG 
GCTCGAGACA AGTTTGAGGA AGACCGTATC TACAGACACT TGGAACCTGC ATTAGCTTTC 
CAGCTGGAGC TCAACCGCAT GCGTAATTTT GCACTGACAG CCATCCCCTG TGCCAATCAT 
AAGATGCACC TGTACTTGGG AGCTGCCCGT GTGGAAGTAG GCACTGAGGT CACAGATTAC 
CGCTTCTTTG TGCGGGCAAT CATCCGTCAC TCAGATTTGG TCACCAAGGA AGCATCATTT 
GAGTACCTGC ACAACGAGGC AGAACGCTTG CTGCTGGAGG CCATGGATGA ACTGGAAGTG 
GCTTTCAACA ACACCACTGT GCGAACCGAC TGCAATCACA TCTTCCTCAA CTTCGTTCCA 
ACAGTCATCA TGGACCCTTC AAAGATTGAG GAATCTGTGC GCTCCATGGT CATGCGCTAC 
GGCAGTCGCT TGTGGAAGCT CCGTGTGCTT CAAGCCGAGC TGAAGATCAA CATCCGATTG 
ACTCCCACCG GCAAACAGAT CCCCATCCGC CTCTTCCTGA CCAACGAGAG TGGCTACTAC 
CTGGACATCA GCCTGTACAA GGAAGTCACT GACTCCCGTA CAGGACAGAT CATGTTCCAG 
GCATATGGAG ATAAGCAGGG TCCTCTTCAT GGCATGCTCA TCAACACACC CTACGTCACA 
AAAGACCTGC TGCAGTCCAA ACGCTTCCAG GCACAGAGCC TCGGCACTAC CTATGTTTAC 
GACTTCCCAG AAATGTTTCG CCAGGCTTTA AGGAAGCTGT GGCAATCCAT GGATGCACAC 
GCCAACTTGC CCAAGTGCCC TCTTCCATCA GAACTGCTCA CCTTCACAGA GCTGGTTCTG 
GATTCTCAGG GTCAGCTGGT GCAGATGAAC CGCCTACCTG GAGGGAATGA GATTGGCATG 
GTAGCATGGC GAATGACCCT CCGGACCCCT GAGTATCCAG CCGGCCGTGA GATTATAGTG 
ATCAGCAATG ACATCACACA TAAGATTGGC TCGTTTGGGC CACAAGAAGA TGTTCTGTTC 
CAGCAAGCTT CAGAAATGGC AAGAGAAAGT GGCATTCCAC GGATCTATAT TGCTGCCAAC 
AGCGGAGCTC GTATAGGCCT GGCAGAGGAG ATCAGACACA TGTTTCATGT GGCCTGGCAG 
GATCCGGTGG ACCCATACAA GGGTTTTAAG TACCTATACC TCACACCACA GGACTATAAG 
AAGGTGTCTG CTCTGAACTC AGTCCACTGT GAACATGTGG AGGATGAGGG GGAATCCAGG 
TATAAAATCA CTGATATCAT TGGGAAAGAG GAAGGGTTGG GTGTGGAGAA CTTGAGAGGT 
TCTGGCATGA TCGCAGGTGA ATCGTCACTG GCGTATGAGG ATATTATCAC CATGAATCTG 
GTGACATGCA GGGCCATTGG AATCGGAGCG TACTTGGTGA GACTGGGCCA AAGAACCATA 
CAGGTGGAAA ACTCTCATAT CATCCTGACT GGAGCCGGAG CTCTCAATAA GGTTCTCGGG 
CGTGAGGTGT ACACTTCCAA TAACCAACTG GGTGGAGTAC AGATCATGCA CAATAACGGA 
GTCACACACA CCAATGTGTG CGATGATTTT GAGGGAGTGT ATACATTGCT GCACTGGCTG 
TCTTACATGC CAAAGAATAT GTCCAGCCCT GTGCCGATAC TCTCTGCTAA AGATCCCATT 
GATCGAGCGA TAGAGTTTGT CCCAACCAAG GCACCTTATG ACCCACGCTG GATGCTAGCT 
GGACGTCCCA ATCAGAACAT TAAGGGTGCA TGGGTGAGTG GGTTCTTCGA CCAGGGCTCT 
TTTCTGGAGA TCATGCAGCC ATGGGCACAG AGTGTTGTCG TAGGACGGGC CAGGTTGGGT 
GGGATCCCAA CTGGAGTAGT TGCCGTGGAA ACCCGATCAG TGGAGCTTTC AATCCCAGCA 
GACCCAGCTA ATCTGGACTC GGAAGCCAAG ATAATCCAGC AGGCTGGGCA AGTGTGGTTC 
CCTGATTCTG CTTTCAAAAC TGCACAAGCC ATTAAAGACT TCAATAGAGA GGGACTTCCG 
CTTATAGTGT TTGCCAACTG GAGAGGTTTT TCTGGAGGCA TGAAAGACAT GTATGACCAG 
GTGCTGAAGT TTGGAGCGTA TATAGTGGAC GGTCTGAGAG AGTACAGACA GCCGGTTCTG 
GTGTATATCC CACCACAGGC GGAGCTCCGG GGAGGATCAT GGGTTGTCAT AGACCCTACT 
ATCAATCCTC GCCACATGGA AATGTATGCA GACAAAGACA GCCGTGGCGG TGTGCTGGAG 
CCTGAAGGCA CAGTGGAGAT CAAGTTCCGC AAAAAAGACC TGGTGAAGAC CATGCGACGG 
GTGGACCCTG TGTACATGGG GCTTGCAGAA AGACTGGGAA CCCCAGAGTT GAGTGTGTCT 
GAGCGTAAAG AGCTGGAGAG TAAACTAAAA GAGAGAGAAG AGTTCCTGCT GCCCATATAT 
CATCAAGTAG CTGTTCAGTT CGCTGACCTT CATGACACAC CGGGCCGCAT GCAGGAGAAA 
GGAGTCATCA CTGATATCCT TGAGTGGAGC ACGTCTCGGC AGTTTTTCTA CTGGCGTTTG 
CGGAGGTTAT TGCTGGAGGA AACGGTGAAG AGGAAGATCC AGTGTGCTAA CAGTGAGCTG 
ACGGACGGAC AGGTTCAGGC TATGCTTCGC CGTTGGTTCG TGGAAGCAGA GGGAGCTGTG 
AAAGCGTATT TGTGGGACAG TAATGAAGAT GTGGTGGAGT GGTTGGAGAG GCAGCTGGCA 
GAGGAAGAAG GCGCCAGATC CGTCATCGAC GAGAATATCA AATATATTCG CAGAGATCAC 
ATCCTCAAAC AGATCCGCAG CCTGGTTCAA GCTAACCCTG AGGTGGCTAT GGACTCCATT 
GTACACATGA CCCAGCACAT TTCCCCCACA CAGCGCGCCG AAGTCGTCCG CATCCTCTCC 
ACCATGGACA CCCCAGCCTC CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_017356113.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211602.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X11, mRNA.

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CloneID	ODa59094
Clone ID Related Accession (Same CDS sequence)	XM_017356113.1 , XM_017356113.2
Accession Version	XM_017356113.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356113.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG AGAGAATTCC CCAAATTTTT TACATTCCGG 
GCTCGAGACA AGTTTGAGGA AGACCGTATC TACAGACACT TGGAACCTGC ATTAGCTTTC 
CAGCTGGAGC TCAACCGCAT GCGTAATTTT GCACTGACAG CCATCCCCTG TGCCAATCAT 
AAGATGCACC TGTACTTGGG AGCTGCCCGT GTGGAAGTAG GCACTGAGGT CACAGATTAC 
CGCTTCTTTG TGCGGGCAAT CATCCGTCAC TCAGATTTGG TCACCAAGGA AGCATCATTT 
GAGTACCTGC ACAACGAGGC AGAACGCTTG CTGCTGGAGG CCATGGATGA ACTGGAAGTG 
GCTTTCAACA ACACCACTGT GCGAACCGAC TGCAATCACA TCTTCCTCAA CTTCGTTCCA 
ACAGTCATCA TGGACCCTTC AAAGATTGAG GAATCTGTGC GCTCCATGGT CATGCGCTAC 
GGCAGTCGCT TGTGGAAGCT CCGTGTGCTT CAAGCCGAGC TGAAGATCAA CATCCGATTG 
ACTCCCACCG GCAAACAGAT CCCCATCCGC CTCTTCCTGA CCAACGAGAG TGGCTACTAC 
CTGGACATCA GCCTGTACAA GGAAGTCACT GACTCCCGTA CAGGACAGAT CATGTTCCAG 
GCATATGGAG ATAAGCAGGG TCCTCTTCAT GGCATGCTCA TCAACACACC CTACGTCACA 
AAAGACCTGC TGCAGTCCAA ACGCTTCCAG GCACAGAGCC TCGGCACTAC CTATGTTTAC 
GACTTCCCAG AAATGTTTCG CCAGGCTTTA AGGAAGCTGT GGCAATCCAT GGATGCACAC 
GCCAACTTGC CCAAGTGCCC TCTTCCATCA GAACTGCTCA CCTTCACAGA GCTGGTTCTG 
GATTCTCAGG GTCAGCTGGT GCAGATGAAC CGCCTACCTG GAGGGAATGA GATTGGCATG 
GTAGCATGGC GAATGACCCT CCGGACCCCT GAGTATCCAG CCGGCCGTGA GATTATAGTG 
ATCAGCAATG ACATCACACA TAAGATTGGC TCGTTTGGGC CACAAGAAGA TGTTCTGTTC 
CAGCAAGCTT CAGAAATGGC AAGAGAAAGT GGCATTCCAC GGATCTATAT TGCTGCCAAC 
AGCGGAGCTC GTATAGGCCT GGCAGAGGAG ATCAGACACA TGTTTCATGT GGCCTGGCAG 
GATCCGGTGG ACCCATACAA GGGTTTTAAG TACCTATACC TCACACCACA GGACTATAAG 
AAGGTGTCTG CTCTGAACTC AGTCCACTGT GAACATGTGG AGGATGAGGG GGAATCCAGG 
TATAAAATCA CTGATATCAT TGGGAAAGAG GAAGGGTTGG GTGTGGAGAA CTTGAGAGGT 
TCTGGCATGA TCGCAGGTGA ATCGTCACTG GCGTATGAGG ATATTATCAC CATGAATCTG 
GTGACATGCA GGGCCATTGG AATCGGAGCG TACTTGGTGA GACTGGGCCA AAGAACCATA 
CAGGTGGAAA ACTCTCATAT CATCCTGACT GGAGCCGGAG CTCTCAATAA GGTTCTCGGG 
CGTGAGGTGT ACACTTCCAA TAACCAACTG GGTGGAGTAC AGATCATGCA CAATAACGGA 
GTCACACACA CCAATGTGTG CGATGATTTT GAGGGAGTGT ATACATTGCT GCACTGGCTG 
TCTTACATGC CAAAGAATAT GTCCAGCCCT GTGCCGATAC TCTCTGCTAA AGATCCCATT 
GATCGAGCGA TAGAGTTTGT CCCAACCAAG GCACCTTATG ACCCACGCTG GATGCTAGCT 
GGACGTCCCA ATCAGAACAT TAAGGGTGCA TGGGTGAGTG GGTTCTTCGA CCAGGGCTCT 
TTTCTGGAGA TCATGCAGCC ATGGGCACAG AGTGTTGTCG TAGGACGGGC CAGGTTGGGT 
GGGATCCCAA CTGGAGTAGT TGCCGTGGAA ACCCGATCAG TGGAGCTTTC AATCCCAGCA 
GACCCAGCTA ATCTGGACTC GGAAGCCAAG ATAATCCAGC AGGCTGGGCA AGTGTGGTTC 
CCTGATTCTG CTTTCAAAAC TGCACAAGCC ATTAAAGACT TCAATAGAGA GGGACTTCCG 
CTTATAGTGT TTGCCAACTG GAGAGGTTTT TCTGGAGGCA TGAAAGACAT GTATGACCAG 
GTGCTGAAGT TTGGAGCGTA TATAGTGGAC GGTCTGAGAG AGTACAGACA GCCGGTTCTG 
GTGTATATCC CACCACAGGC GGAGCTCCGG GGAGGATCAT GGGTTGTCAT AGACCCTACT 
ATCAATCCTC GCCACATGGA AATGTATGCA GACAAAGACA GCCGTGGCGG TGTGCTGGAG 
CCTGAAGGCA CAGTGGAGAT CAAGTTCCGC AAAAAAGACC TGGTGAAGAC CATGCGACGG 
GTGGACCCTG TGTACATGGG GCTTGCAGAA AGACTGGGAA CCCCAGAGTT GAGTGTGTCT 
GAGCGTAAAG AGCTGGAGAG TAAACTAAAA GAGAGAGAAG AGTTCCTGCT GCCCATATAT 
CATCAAGTAG CTGTTCAGTT CGCTGACCTT CATGACACAC CGGGCCGCAT GCAGGAGAAA 
GGAGTCATCA CTGATATCCT TGAGTGGAGC ACGTCTCGGC AGTTTTTCTA CTGGCGTTTG 
CGGAGGTTAT TGCTGGAGGA AACGGTGAAG AGGAAGATCC AGTGTGCTAA CAGTGAGCTG 
ACGGACGGAC AGGTTCAGGC TATGCTTCGC CGTTGGTTCG TGGAAGCAGA GGGAGCTGTG 
AAAGCGTATT TGTGGGACAG TAATGAAGAT GTGGTGGAGT GGTTGGAGAG GCAGCTGGCA 
GAGGAAGAAG GCGCCAGATC CGTCATCGAC GAGAATATCA AATATATTCG CAGAGATCAC 
ATCCTCAAAC AGATCCGCAG CCTGGTTCAA GCTAACCCTG AGGTGGCTAT GGACTCCATT 
GTACACATGA CCCAGCACAT TTCCCCCACA CAGCGCGCCG AAGTCGTCCG CATCCTCTCC 
ACCATGGACA CCCCAGCCTC CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211602.1
CDS	237..7343
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRKLSAPLQDYKPPANTANTSGATEAASDSAESEDRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_017356113.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356113.2

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG AGAGAATTCC CCAAATTTTT TACATTCCGG 
GCTCGAGACA AGTTTGAGGA AGACCGTATC TACAGACACT TGGAACCTGC ATTAGCTTTC 
CAGCTGGAGC TCAACCGCAT GCGTAATTTT GCACTGACAG CCATCCCCTG TGCCAATCAT 
AAGATGCACC TGTACTTGGG AGCTGCCCGT GTGGAAGTAG GCACTGAGGT CACAGATTAC 
CGCTTCTTTG TGCGGGCAAT CATCCGTCAC TCAGATTTGG TCACCAAGGA AGCATCATTT 
GAGTACCTGC ACAACGAGGC AGAACGCTTG CTGCTGGAGG CCATGGATGA ACTGGAAGTG 
GCTTTCAACA ACACCACTGT GCGAACCGAC TGCAATCACA TCTTCCTCAA CTTCGTTCCA 
ACAGTCATCA TGGACCCTTC AAAGATTGAG GAATCTGTGC GCTCCATGGT CATGCGCTAC 
GGCAGTCGCT TGTGGAAGCT CCGTGTGCTT CAAGCCGAGC TGAAGATCAA CATCCGATTG 
ACTCCCACCG GCAAACAGAT CCCCATCCGC CTCTTCCTGA CCAACGAGAG TGGCTACTAC 
CTGGACATCA GCCTGTACAA GGAAGTCACT GACTCCCGTA CAGGACAGAT CATGTTCCAG 
GCATATGGAG ATAAGCAGGG TCCTCTTCAT GGCATGCTCA TCAACACACC CTACGTCACA 
AAAGACCTGC TGCAGTCCAA ACGCTTCCAG GCACAGAGCC TCGGCACTAC CTATGTTTAC 
GACTTCCCAG AAATGTTTCG CCAGGCTTTA AGGAAGCTGT GGCAATCCAT GGATGCACAC 
GCCAACTTGC CCAAGTGCCC TCTTCCATCA GAACTGCTCA CCTTCACAGA GCTGGTTCTG 
GATTCTCAGG GTCAGCTGGT GCAGATGAAC CGCCTACCTG GAGGGAATGA GATTGGCATG 
GTAGCATGGC GAATGACCCT CCGGACCCCT GAGTATCCAG CCGGCCGTGA GATTATAGTG 
ATCAGCAATG ACATCACACA TAAGATTGGC TCGTTTGGGC CACAAGAAGA TGTTCTGTTC 
CAGCAAGCTT CAGAAATGGC AAGAGAAAGT GGCATTCCAC GGATCTATAT TGCTGCCAAC 
AGCGGAGCTC GTATAGGCCT GGCAGAGGAG ATCAGACACA TGTTTCATGT GGCCTGGCAG 
GATCCGGTGG ACCCATACAA GGGTTTTAAG TACCTATACC TCACACCACA GGACTATAAG 
AAGGTGTCTG CTCTGAACTC AGTCCACTGT GAACATGTGG AGGATGAGGG GGAATCCAGG 
TATAAAATCA CTGATATCAT TGGGAAAGAG GAAGGGTTGG GTGTGGAGAA CTTGAGAGGT 
TCTGGCATGA TCGCAGGTGA ATCGTCACTG GCGTATGAGG ATATTATCAC CATGAATCTG 
GTGACATGCA GGGCCATTGG AATCGGAGCG TACTTGGTGA GACTGGGCCA AAGAACCATA 
CAGGTGGAAA ACTCTCATAT CATCCTGACT GGAGCCGGAG CTCTCAATAA GGTTCTCGGG 
CGTGAGGTGT ACACTTCCAA TAACCAACTG GGTGGAGTAC AGATCATGCA CAATAACGGA 
GTCACACACA CCAATGTGTG CGATGATTTT GAGGGAGTGT ATACATTGCT GCACTGGCTG 
TCTTACATGC CAAAGAATAT GTCCAGCCCT GTGCCGATAC TCTCTGCTAA AGATCCCATT 
GATCGAGCGA TAGAGTTTGT CCCAACCAAG GCACCTTATG ACCCACGCTG GATGCTAGCT 
GGACGTCCCA ATCAGAACAT TAAGGGTGCA TGGGTGAGTG GGTTCTTCGA CCAGGGCTCT 
TTTCTGGAGA TCATGCAGCC ATGGGCACAG AGTGTTGTCG TAGGACGGGC CAGGTTGGGT 
GGGATCCCAA CTGGAGTAGT TGCCGTGGAA ACCCGATCAG TGGAGCTTTC AATCCCAGCA 
GACCCAGCTA ATCTGGACTC GGAAGCCAAG ATAATCCAGC AGGCTGGGCA AGTGTGGTTC 
CCTGATTCTG CTTTCAAAAC TGCACAAGCC ATTAAAGACT TCAATAGAGA GGGACTTCCG 
CTTATAGTGT TTGCCAACTG GAGAGGTTTT TCTGGAGGCA TGAAAGACAT GTATGACCAG 
GTGCTGAAGT TTGGAGCGTA TATAGTGGAC GGTCTGAGAG AGTACAGACA GCCGGTTCTG 
GTGTATATCC CACCACAGGC GGAGCTCCGG GGAGGATCAT GGGTTGTCAT AGACCCTACT 
ATCAATCCTC GCCACATGGA AATGTATGCA GACAAAGACA GCCGTGGCGG TGTGCTGGAG 
CCTGAAGGCA CAGTGGAGAT CAAGTTCCGC AAAAAAGACC TGGTGAAGAC CATGCGACGG 
GTGGACCCTG TGTACATGGG GCTTGCAGAA AGACTGGGAA CCCCAGAGTT GAGTGTGTCT 
GAGCGTAAAG AGCTGGAGAG TAAACTAAAA GAGAGAGAAG AGTTCCTGCT GCCCATATAT 
CATCAAGTAG CTGTTCAGTT CGCTGACCTT CATGACACAC CGGGCCGCAT GCAGGAGAAA 
GGAGTCATCA CTGATATCCT TGAGTGGAGC ACGTCTCGGC AGTTTTTCTA CTGGCGTTTG 
CGGAGGTTAT TGCTGGAGGA AACGGTGAAG AGGAAGATCC AGTGTGCTAA CAGTGAGCTG 
ACGGACGGAC AGGTTCAGGC TATGCTTCGC CGTTGGTTCG TGGAAGCAGA GGGAGCTGTG 
AAAGCGTATT TGTGGGACAG TAATGAAGAT GTGGTGGAGT GGTTGGAGAG GCAGCTGGCA 
GAGGAAGAAG GCGCCAGATC CGTCATCGAC GAGAATATCA AATATATTCG CAGAGATCAC 
ATCCTCAAAC AGATCCGCAG CCTGGTTCAA GCTAACCCTG AGGTGGCTAT GGACTCCATT 
GTACACATGA CCCAGCACAT TTCCCCCACA CAGCGCGCCG AAGTCGTCCG CATCCTCTCC 
ACCATGGACA CCCCAGCCTC CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_017356114.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211603.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X6, mRNA.

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CloneID	ODa59095
Clone ID Related Accession (Same CDS sequence)	XM_017356114.1 , XM_017356114.2
Accession Version	XM_017356114.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGAATG 
TCCTTTTCCT CAAACCTGAA CCACTACGGC ATGGTCCACG TGGCCAGCGT TAGTGATGTG 
CTCCTAGACA CCTCCTTTAC CCCACCCTGT CAGCGCATGG GAGCCATGGT TTCCTTCCGC 
TCTTTCCAGG AGTTCACCAG GAACATAAAG GATGTTCTGA GCTGTTTCTC AGACTCTCCT 
CCATCAACTC CAACCTTTCC TGAAGGGGGG AACCCTGTGC TGTATAGAGA GGAGGACAGC 
AAGAGCATTC AAGATGAGCC CATTCATATT CTTAATGTGG CAATAAAGAC GGACAGTGAT 
ATTGATGATG ATGGGCTGGC GGCCATGTTT CGTGAGTTTA CACAGTCTAA GAAATCTCTG 
CTGTTTGACC ACGGCATCCG AAGACTTACC TTCCTGGTTG CTCAGAAGGA TTTCAGAAAG 
CAAATCAACT GTGAGGTGGA CCAGAGGTTC CATAGAGAAT TCCCCAAATT TTTTACATTC 
CGGGCTCGAG ACAAGTTTGA GGAAGACCGT ATCTACAGAC ACTTGGAACC TGCATTAGCT 
TTCCAGCTGG AGCTCAACCG CATGCGTAAT TTTGCACTGA CAGCCATCCC CTGTGCCAAT 
CATAAGATGC ACCTGTACTT GGGAGCTGCC CGTGTGGAAG TAGGCACTGA GGTCACAGAT 
TACCGCTTCT TTGTGCGGGC AATCATCCGT CACTCAGATT TGGTCACCAA GGAAGCATCA 
TTTGAGTACC TGCACAACGA GGCAGAACGC TTGCTGCTGG AGGCCATGGA TGAACTGGAA 
GTGGCTTTCA ACAACACCAC TGTGCGAACC GACTGCAATC ACATCTTCCT CAACTTCGTT 
CCAACAGTCA TCATGGACCC TTCAAAGATT GAGGAATCTG TGCGCTCCAT GGTCATGCGC 
TACGGCAGTC GCTTGTGGAA GCTCCGTGTG CTTCAAGCCG AGCTGAAGAT CAACATCCGA 
TTGACTCCCA CCGGCAAACA GATCCCCATC CGCCTCTTCC TGACCAACGA GAGTGGCTAC 
TACCTGGACA TCAGCCTGTA CAAGGAAGTC ACTGACTCCC GTACAGGACA GGTGGGGCAC 
AAAGACCGAC AGATCATGTT CCAGGCATAT GGAGATAAGC AGGGTCCTCT TCATGGCATG 
CTCATCAACA CACCCTACGT CACAAAAGAC CTGCTGCAGT CCAAACGCTT CCAGGCACAG 
AGCCTCGGCA CTACCTATGT TTACGACTTC CCAGAAATGT TTCGCCAGGC TTTAAGGAAG 
CTGTGGCAAT CCATGGATGC ACACGCCAAC TTGCCCAAGT GCCCTCTTCC ATCAGAACTG 
CTCACCTTCA CAGAGCTGGT TCTGGATTCT CAGGGTCAGC TGGTGCAGAT GAACCGCCTA 
CCTGGAGGGA ATGAGATTGG CATGGTAGCA TGGCGAATGA CCCTCCGGAC CCCTGAGTAT 
CCAGCCGGCC GTGAGATTAT AGTGATCAGC AATGACATCA CACATAAGAT TGGCTCGTTT 
GGGCCACAAG AAGATGTTCT GTTCCAGCAA GCTTCAGAAA TGGCAAGAGA AAGTGGCATT 
CCACGGATCT ATATTGCTGC CAACAGCGGA GCTCGTATAG GCCTGGCAGA GGAGATCAGA 
CACATGTTTC ATGTGGCCTG GCAGGATCCG GTGGACCCAT ACAAGGGTTT TAAGTACCTA 
TACCTCACAC CACAGGACTA TAAGAAGGTG TCTGCTCTGA ACTCAGTCCA CTGTGAACAT 
GTGGAGGATG AGGGGGAATC CAGGTATAAA ATCACTGATA TCATTGGGAA AGAGGAAGGG 
TTGGGTGTGG AGAACTTGAG AGGTTCTGGC ATGATCGCAG GTGAATCGTC ACTGGCGTAT 
GAGGATATTA TCACCATGAA TCTGGTGACA TGCAGGGCCA TTGGAATCGG AGCGTACTTG 
GTGAGACTGG GCCAAAGAAC CATACAGGTG GAAAACTCTC ATATCATCCT GACTGGAGCC 
GGAGCTCTCA ATAAGGTTCT CGGGCGTGAG GTGTACACTT CCAATAACCA ACTGGGTGGA 
GTACAGATCA TGCACAATAA CGGAGTCACA CACACCAATG TGTGCGATGA TTTTGAGGGA 
GTGTATACAT TGCTGCACTG GCTGTCTTAC ATGCCAAAGA ATATGTCCAG CCCTGTGCCG 
ATACTCTCTG CTAAAGATCC CATTGATCGA GCGATAGAGT TTGTCCCAAC CAAGGCACCT 
TATGACCCAC GCTGGATGCT AGCTGGACGT CCCAATCAGA ACATTAAGGG TGCATGGGTG 
AGTGGGTTCT TCGACCAGGG CTCTTTTCTG GAGATCATGC AGCCATGGGC ACAGAGTGTT 
GTCGTAGGAC GGGCCAGGTT GGGTGGGATC CCAACTGGAG TAGTTGCCGT GGAAACCCGA 
TCAGTGGAGC TTTCAATCCC AGCAGACCCA GCTAATCTGG ACTCGGAAGC CAAGATAATC 
CAGCAGGCTG GGCAAGTGTG GTTCCCTGAT TCTGCTTTCA AAACTGCACA AGCCATTAAA 
GACTTCAATA GAGAGGGACT TCCGCTTATA GTGTTTGCCA ACTGGAGAGG TTTTTCTGGA 
GGCATGAAAG ACATGTATGA CCAGGTGCTG AAGTTTGGAG CGTATATAGT GGACGGTCTG 
AGAGAGTACA GACAGCCGGT TCTGGTGTAT ATCCCACCAC AGGCGGAGCT CCGGGGAGGA 
TCATGGGTTG TCATAGACCC TACTATCAAT CCTCGCCACA TGGAAATGTA TGCAGACAAA 
GACAGCCGTG GCGGTGTGCT GGAGCCTGAA GGCACAGTGG AGATCAAGTT CCGCAAAAAA 
GACCTGGTGA AGACCATGCG ACGGGTGGAC CCTGTGTACA TGGGGCTTGC AGAAAGACTG 
GGAACCCCAG AGTTGAGTGT GTCTGAGCGT AAAGAGCTGG AGAGTAAACT AAAAGAGAGA 
GAAGAGTTCC TGCTGCCCAT ATATCATCAA GTAGCTGTTC AGTTCGCTGA CCTTCATGAC 
ACACCGGGCC GCATGCAGGA GAAAGGAGTC ATCACTGATA TCCTTGAGTG GAGCACGTCT 
CGGCAGTTTT TCTACTGGCG TTTGCGGAGG TTATTGCTGG AGGAAACGGT GAAGAGGAAG 
ATCCAGTGTG CTAACAGTGA GCTGACGGAC GGACAGGTTC AGGCTATGCT TCGCCGTTGG 
TTCGTGGAAG CAGAGGGAGC TGTGAAAGCG TATTTGTGGG ACAGTAATGA AGATGTGGTG 
GAGTGGTTGG AGAGGCAGCT GGCAGAGGAA GAAGGCGCCA GATCCGTCAT CGACGAGAAT 
ATCAAATATA TTCGCAGAGA TCACATCCTC AAACAGATCC GCAGCCTGGT TCAAGCTAAC 
CCTGAGGTGG CTATGGACTC CATTGTACAC ATGACCCAGC ACATTTCCCC CACACAGCGC 
GCCGAAGTCG TCCGCATCCT CTCCACCATG GACACCCCAG CCTCCTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211603.1
CDS	242..7312
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_017356114.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356114.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGAATG 
TCCTTTTCCT CAAACCTGAA CCACTACGGC ATGGTCCACG TGGCCAGCGT TAGTGATGTG 
CTCCTAGACA CCTCCTTTAC CCCACCCTGT CAGCGCATGG GAGCCATGGT TTCCTTCCGC 
TCTTTCCAGG AGTTCACCAG GAACATAAAG GATGTTCTGA GCTGTTTCTC AGACTCTCCT 
CCATCAACTC CAACCTTTCC TGAAGGGGGG AACCCTGTGC TGTATAGAGA GGAGGACAGC 
AAGAGCATTC AAGATGAGCC CATTCATATT CTTAATGTGG CAATAAAGAC GGACAGTGAT 
ATTGATGATG ATGGGCTGGC GGCCATGTTT CGTGAGTTTA CACAGTCTAA GAAATCTCTG 
CTGTTTGACC ACGGCATCCG AAGACTTACC TTCCTGGTTG CTCAGAAGGA TTTCAGAAAG 
CAAATCAACT GTGAGGTGGA CCAGAGGTTC CATAGAGAAT TCCCCAAATT TTTTACATTC 
CGGGCTCGAG ACAAGTTTGA GGAAGACCGT ATCTACAGAC ACTTGGAACC TGCATTAGCT 
TTCCAGCTGG AGCTCAACCG CATGCGTAAT TTTGCACTGA CAGCCATCCC CTGTGCCAAT 
CATAAGATGC ACCTGTACTT GGGAGCTGCC CGTGTGGAAG TAGGCACTGA GGTCACAGAT 
TACCGCTTCT TTGTGCGGGC AATCATCCGT CACTCAGATT TGGTCACCAA GGAAGCATCA 
TTTGAGTACC TGCACAACGA GGCAGAACGC TTGCTGCTGG AGGCCATGGA TGAACTGGAA 
GTGGCTTTCA ACAACACCAC TGTGCGAACC GACTGCAATC ACATCTTCCT CAACTTCGTT 
CCAACAGTCA TCATGGACCC TTCAAAGATT GAGGAATCTG TGCGCTCCAT GGTCATGCGC 
TACGGCAGTC GCTTGTGGAA GCTCCGTGTG CTTCAAGCCG AGCTGAAGAT CAACATCCGA 
TTGACTCCCA CCGGCAAACA GATCCCCATC CGCCTCTTCC TGACCAACGA GAGTGGCTAC 
TACCTGGACA TCAGCCTGTA CAAGGAAGTC ACTGACTCCC GTACAGGACA GGTGGGGCAC 
AAAGACCGAC AGATCATGTT CCAGGCATAT GGAGATAAGC AGGGTCCTCT TCATGGCATG 
CTCATCAACA CACCCTACGT CACAAAAGAC CTGCTGCAGT CCAAACGCTT CCAGGCACAG 
AGCCTCGGCA CTACCTATGT TTACGACTTC CCAGAAATGT TTCGCCAGGC TTTAAGGAAG 
CTGTGGCAAT CCATGGATGC ACACGCCAAC TTGCCCAAGT GCCCTCTTCC ATCAGAACTG 
CTCACCTTCA CAGAGCTGGT TCTGGATTCT CAGGGTCAGC TGGTGCAGAT GAACCGCCTA 
CCTGGAGGGA ATGAGATTGG CATGGTAGCA TGGCGAATGA CCCTCCGGAC CCCTGAGTAT 
CCAGCCGGCC GTGAGATTAT AGTGATCAGC AATGACATCA CACATAAGAT TGGCTCGTTT 
GGGCCACAAG AAGATGTTCT GTTCCAGCAA GCTTCAGAAA TGGCAAGAGA AAGTGGCATT 
CCACGGATCT ATATTGCTGC CAACAGCGGA GCTCGTATAG GCCTGGCAGA GGAGATCAGA 
CACATGTTTC ATGTGGCCTG GCAGGATCCG GTGGACCCAT ACAAGGGTTT TAAGTACCTA 
TACCTCACAC CACAGGACTA TAAGAAGGTG TCTGCTCTGA ACTCAGTCCA CTGTGAACAT 
GTGGAGGATG AGGGGGAATC CAGGTATAAA ATCACTGATA TCATTGGGAA AGAGGAAGGG 
TTGGGTGTGG AGAACTTGAG AGGTTCTGGC ATGATCGCAG GTGAATCGTC ACTGGCGTAT 
GAGGATATTA TCACCATGAA TCTGGTGACA TGCAGGGCCA TTGGAATCGG AGCGTACTTG 
GTGAGACTGG GCCAAAGAAC CATACAGGTG GAAAACTCTC ATATCATCCT GACTGGAGCC 
GGAGCTCTCA ATAAGGTTCT CGGGCGTGAG GTGTACACTT CCAATAACCA ACTGGGTGGA 
GTACAGATCA TGCACAATAA CGGAGTCACA CACACCAATG TGTGCGATGA TTTTGAGGGA 
GTGTATACAT TGCTGCACTG GCTGTCTTAC ATGCCAAAGA ATATGTCCAG CCCTGTGCCG 
ATACTCTCTG CTAAAGATCC CATTGATCGA GCGATAGAGT TTGTCCCAAC CAAGGCACCT 
TATGACCCAC GCTGGATGCT AGCTGGACGT CCCAATCAGA ACATTAAGGG TGCATGGGTG 
AGTGGGTTCT TCGACCAGGG CTCTTTTCTG GAGATCATGC AGCCATGGGC ACAGAGTGTT 
GTCGTAGGAC GGGCCAGGTT GGGTGGGATC CCAACTGGAG TAGTTGCCGT GGAAACCCGA 
TCAGTGGAGC TTTCAATCCC AGCAGACCCA GCTAATCTGG ACTCGGAAGC CAAGATAATC 
CAGCAGGCTG GGCAAGTGTG GTTCCCTGAT TCTGCTTTCA AAACTGCACA AGCCATTAAA 
GACTTCAATA GAGAGGGACT TCCGCTTATA GTGTTTGCCA ACTGGAGAGG TTTTTCTGGA 
GGCATGAAAG ACATGTATGA CCAGGTGCTG AAGTTTGGAG CGTATATAGT GGACGGTCTG 
AGAGAGTACA GACAGCCGGT TCTGGTGTAT ATCCCACCAC AGGCGGAGCT CCGGGGAGGA 
TCATGGGTTG TCATAGACCC TACTATCAAT CCTCGCCACA TGGAAATGTA TGCAGACAAA 
GACAGCCGTG GCGGTGTGCT GGAGCCTGAA GGCACAGTGG AGATCAAGTT CCGCAAAAAA 
GACCTGGTGA AGACCATGCG ACGGGTGGAC CCTGTGTACA TGGGGCTTGC AGAAAGACTG 
GGAACCCCAG AGTTGAGTGT GTCTGAGCGT AAAGAGCTGG AGAGTAAACT AAAAGAGAGA 
GAAGAGTTCC TGCTGCCCAT ATATCATCAA GTAGCTGTTC AGTTCGCTGA CCTTCATGAC 
ACACCGGGCC GCATGCAGGA GAAAGGAGTC ATCACTGATA TCCTTGAGTG GAGCACGTCT 
CGGCAGTTTT TCTACTGGCG TTTGCGGAGG TTATTGCTGG AGGAAACGGT GAAGAGGAAG 
ATCCAGTGTG CTAACAGTGA GCTGACGGAC GGACAGGTTC AGGCTATGCT TCGCCGTTGG 
TTCGTGGAAG CAGAGGGAGC TGTGAAAGCG TATTTGTGGG ACAGTAATGA AGATGTGGTG 
GAGTGGTTGG AGAGGCAGCT GGCAGAGGAA GAAGGCGCCA GATCCGTCAT CGACGAGAAT 
ATCAAATATA TTCGCAGAGA TCACATCCTC AAACAGATCC GCAGCCTGGT TCAAGCTAAC 
CCTGAGGTGG CTATGGACTC CATTGTACAC ATGACCCAGC ACATTTCCCC CACACAGCGC 
GCCGAAGTCG TCCGCATCCT CTCCACCATG GACACCCCAG CCTCCTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_017356114.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211603.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X12, mRNA.

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CloneID	ODa59095
Clone ID Related Accession (Same CDS sequence)	XM_017356114.1 , XM_017356114.2
Accession Version	XM_017356114.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356114.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGAATG 
TCCTTTTCCT CAAACCTGAA CCACTACGGC ATGGTCCACG TGGCCAGCGT TAGTGATGTG 
CTCCTAGACA CCTCCTTTAC CCCACCCTGT CAGCGCATGG GAGCCATGGT TTCCTTCCGC 
TCTTTCCAGG AGTTCACCAG GAACATAAAG GATGTTCTGA GCTGTTTCTC AGACTCTCCT 
CCATCAACTC CAACCTTTCC TGAAGGGGGG AACCCTGTGC TGTATAGAGA GGAGGACAGC 
AAGAGCATTC AAGATGAGCC CATTCATATT CTTAATGTGG CAATAAAGAC GGACAGTGAT 
ATTGATGATG ATGGGCTGGC GGCCATGTTT CGTGAGTTTA CACAGTCTAA GAAATCTCTG 
CTGTTTGACC ACGGCATCCG AAGACTTACC TTCCTGGTTG CTCAGAAGGA TTTCAGAAAG 
CAAATCAACT GTGAGGTGGA CCAGAGGTTC CATAGAGAAT TCCCCAAATT TTTTACATTC 
CGGGCTCGAG ACAAGTTTGA GGAAGACCGT ATCTACAGAC ACTTGGAACC TGCATTAGCT 
TTCCAGCTGG AGCTCAACCG CATGCGTAAT TTTGCACTGA CAGCCATCCC CTGTGCCAAT 
CATAAGATGC ACCTGTACTT GGGAGCTGCC CGTGTGGAAG TAGGCACTGA GGTCACAGAT 
TACCGCTTCT TTGTGCGGGC AATCATCCGT CACTCAGATT TGGTCACCAA GGAAGCATCA 
TTTGAGTACC TGCACAACGA GGCAGAACGC TTGCTGCTGG AGGCCATGGA TGAACTGGAA 
GTGGCTTTCA ACAACACCAC TGTGCGAACC GACTGCAATC ACATCTTCCT CAACTTCGTT 
CCAACAGTCA TCATGGACCC TTCAAAGATT GAGGAATCTG TGCGCTCCAT GGTCATGCGC 
TACGGCAGTC GCTTGTGGAA GCTCCGTGTG CTTCAAGCCG AGCTGAAGAT CAACATCCGA 
TTGACTCCCA CCGGCAAACA GATCCCCATC CGCCTCTTCC TGACCAACGA GAGTGGCTAC 
TACCTGGACA TCAGCCTGTA CAAGGAAGTC ACTGACTCCC GTACAGGACA GGTGGGGCAC 
AAAGACCGAC AGATCATGTT CCAGGCATAT GGAGATAAGC AGGGTCCTCT TCATGGCATG 
CTCATCAACA CACCCTACGT CACAAAAGAC CTGCTGCAGT CCAAACGCTT CCAGGCACAG 
AGCCTCGGCA CTACCTATGT TTACGACTTC CCAGAAATGT TTCGCCAGGC TTTAAGGAAG 
CTGTGGCAAT CCATGGATGC ACACGCCAAC TTGCCCAAGT GCCCTCTTCC ATCAGAACTG 
CTCACCTTCA CAGAGCTGGT TCTGGATTCT CAGGGTCAGC TGGTGCAGAT GAACCGCCTA 
CCTGGAGGGA ATGAGATTGG CATGGTAGCA TGGCGAATGA CCCTCCGGAC CCCTGAGTAT 
CCAGCCGGCC GTGAGATTAT AGTGATCAGC AATGACATCA CACATAAGAT TGGCTCGTTT 
GGGCCACAAG AAGATGTTCT GTTCCAGCAA GCTTCAGAAA TGGCAAGAGA AAGTGGCATT 
CCACGGATCT ATATTGCTGC CAACAGCGGA GCTCGTATAG GCCTGGCAGA GGAGATCAGA 
CACATGTTTC ATGTGGCCTG GCAGGATCCG GTGGACCCAT ACAAGGGTTT TAAGTACCTA 
TACCTCACAC CACAGGACTA TAAGAAGGTG TCTGCTCTGA ACTCAGTCCA CTGTGAACAT 
GTGGAGGATG AGGGGGAATC CAGGTATAAA ATCACTGATA TCATTGGGAA AGAGGAAGGG 
TTGGGTGTGG AGAACTTGAG AGGTTCTGGC ATGATCGCAG GTGAATCGTC ACTGGCGTAT 
GAGGATATTA TCACCATGAA TCTGGTGACA TGCAGGGCCA TTGGAATCGG AGCGTACTTG 
GTGAGACTGG GCCAAAGAAC CATACAGGTG GAAAACTCTC ATATCATCCT GACTGGAGCC 
GGAGCTCTCA ATAAGGTTCT CGGGCGTGAG GTGTACACTT CCAATAACCA ACTGGGTGGA 
GTACAGATCA TGCACAATAA CGGAGTCACA CACACCAATG TGTGCGATGA TTTTGAGGGA 
GTGTATACAT TGCTGCACTG GCTGTCTTAC ATGCCAAAGA ATATGTCCAG CCCTGTGCCG 
ATACTCTCTG CTAAAGATCC CATTGATCGA GCGATAGAGT TTGTCCCAAC CAAGGCACCT 
TATGACCCAC GCTGGATGCT AGCTGGACGT CCCAATCAGA ACATTAAGGG TGCATGGGTG 
AGTGGGTTCT TCGACCAGGG CTCTTTTCTG GAGATCATGC AGCCATGGGC ACAGAGTGTT 
GTCGTAGGAC GGGCCAGGTT GGGTGGGATC CCAACTGGAG TAGTTGCCGT GGAAACCCGA 
TCAGTGGAGC TTTCAATCCC AGCAGACCCA GCTAATCTGG ACTCGGAAGC CAAGATAATC 
CAGCAGGCTG GGCAAGTGTG GTTCCCTGAT TCTGCTTTCA AAACTGCACA AGCCATTAAA 
GACTTCAATA GAGAGGGACT TCCGCTTATA GTGTTTGCCA ACTGGAGAGG TTTTTCTGGA 
GGCATGAAAG ACATGTATGA CCAGGTGCTG AAGTTTGGAG CGTATATAGT GGACGGTCTG 
AGAGAGTACA GACAGCCGGT TCTGGTGTAT ATCCCACCAC AGGCGGAGCT CCGGGGAGGA 
TCATGGGTTG TCATAGACCC TACTATCAAT CCTCGCCACA TGGAAATGTA TGCAGACAAA 
GACAGCCGTG GCGGTGTGCT GGAGCCTGAA GGCACAGTGG AGATCAAGTT CCGCAAAAAA 
GACCTGGTGA AGACCATGCG ACGGGTGGAC CCTGTGTACA TGGGGCTTGC AGAAAGACTG 
GGAACCCCAG AGTTGAGTGT GTCTGAGCGT AAAGAGCTGG AGAGTAAACT AAAAGAGAGA 
GAAGAGTTCC TGCTGCCCAT ATATCATCAA GTAGCTGTTC AGTTCGCTGA CCTTCATGAC 
ACACCGGGCC GCATGCAGGA GAAAGGAGTC ATCACTGATA TCCTTGAGTG GAGCACGTCT 
CGGCAGTTTT TCTACTGGCG TTTGCGGAGG TTATTGCTGG AGGAAACGGT GAAGAGGAAG 
ATCCAGTGTG CTAACAGTGA GCTGACGGAC GGACAGGTTC AGGCTATGCT TCGCCGTTGG 
TTCGTGGAAG CAGAGGGAGC TGTGAAAGCG TATTTGTGGG ACAGTAATGA AGATGTGGTG 
GAGTGGTTGG AGAGGCAGCT GGCAGAGGAA GAAGGCGCCA GATCCGTCAT CGACGAGAAT 
ATCAAATATA TTCGCAGAGA TCACATCCTC AAACAGATCC GCAGCCTGGT TCAAGCTAAC 
CCTGAGGTGG CTATGGACTC CATTGTACAC ATGACCCAGC ACATTTCCCC CACACAGCGC 
GCCGAAGTCG TCCGCATCCT CTCCACCATG GACACCCCAG CCTCCTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211603.1
CDS	236..7306
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_017356114.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356114.2

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGAATG 
TCCTTTTCCT CAAACCTGAA CCACTACGGC ATGGTCCACG TGGCCAGCGT TAGTGATGTG 
CTCCTAGACA CCTCCTTTAC CCCACCCTGT CAGCGCATGG GAGCCATGGT TTCCTTCCGC 
TCTTTCCAGG AGTTCACCAG GAACATAAAG GATGTTCTGA GCTGTTTCTC AGACTCTCCT 
CCATCAACTC CAACCTTTCC TGAAGGGGGG AACCCTGTGC TGTATAGAGA GGAGGACAGC 
AAGAGCATTC AAGATGAGCC CATTCATATT CTTAATGTGG CAATAAAGAC GGACAGTGAT 
ATTGATGATG ATGGGCTGGC GGCCATGTTT CGTGAGTTTA CACAGTCTAA GAAATCTCTG 
CTGTTTGACC ACGGCATCCG AAGACTTACC TTCCTGGTTG CTCAGAAGGA TTTCAGAAAG 
CAAATCAACT GTGAGGTGGA CCAGAGGTTC CATAGAGAAT TCCCCAAATT TTTTACATTC 
CGGGCTCGAG ACAAGTTTGA GGAAGACCGT ATCTACAGAC ACTTGGAACC TGCATTAGCT 
TTCCAGCTGG AGCTCAACCG CATGCGTAAT TTTGCACTGA CAGCCATCCC CTGTGCCAAT 
CATAAGATGC ACCTGTACTT GGGAGCTGCC CGTGTGGAAG TAGGCACTGA GGTCACAGAT 
TACCGCTTCT TTGTGCGGGC AATCATCCGT CACTCAGATT TGGTCACCAA GGAAGCATCA 
TTTGAGTACC TGCACAACGA GGCAGAACGC TTGCTGCTGG AGGCCATGGA TGAACTGGAA 
GTGGCTTTCA ACAACACCAC TGTGCGAACC GACTGCAATC ACATCTTCCT CAACTTCGTT 
CCAACAGTCA TCATGGACCC TTCAAAGATT GAGGAATCTG TGCGCTCCAT GGTCATGCGC 
TACGGCAGTC GCTTGTGGAA GCTCCGTGTG CTTCAAGCCG AGCTGAAGAT CAACATCCGA 
TTGACTCCCA CCGGCAAACA GATCCCCATC CGCCTCTTCC TGACCAACGA GAGTGGCTAC 
TACCTGGACA TCAGCCTGTA CAAGGAAGTC ACTGACTCCC GTACAGGACA GGTGGGGCAC 
AAAGACCGAC AGATCATGTT CCAGGCATAT GGAGATAAGC AGGGTCCTCT TCATGGCATG 
CTCATCAACA CACCCTACGT CACAAAAGAC CTGCTGCAGT CCAAACGCTT CCAGGCACAG 
AGCCTCGGCA CTACCTATGT TTACGACTTC CCAGAAATGT TTCGCCAGGC TTTAAGGAAG 
CTGTGGCAAT CCATGGATGC ACACGCCAAC TTGCCCAAGT GCCCTCTTCC ATCAGAACTG 
CTCACCTTCA CAGAGCTGGT TCTGGATTCT CAGGGTCAGC TGGTGCAGAT GAACCGCCTA 
CCTGGAGGGA ATGAGATTGG CATGGTAGCA TGGCGAATGA CCCTCCGGAC CCCTGAGTAT 
CCAGCCGGCC GTGAGATTAT AGTGATCAGC AATGACATCA CACATAAGAT TGGCTCGTTT 
GGGCCACAAG AAGATGTTCT GTTCCAGCAA GCTTCAGAAA TGGCAAGAGA AAGTGGCATT 
CCACGGATCT ATATTGCTGC CAACAGCGGA GCTCGTATAG GCCTGGCAGA GGAGATCAGA 
CACATGTTTC ATGTGGCCTG GCAGGATCCG GTGGACCCAT ACAAGGGTTT TAAGTACCTA 
TACCTCACAC CACAGGACTA TAAGAAGGTG TCTGCTCTGA ACTCAGTCCA CTGTGAACAT 
GTGGAGGATG AGGGGGAATC CAGGTATAAA ATCACTGATA TCATTGGGAA AGAGGAAGGG 
TTGGGTGTGG AGAACTTGAG AGGTTCTGGC ATGATCGCAG GTGAATCGTC ACTGGCGTAT 
GAGGATATTA TCACCATGAA TCTGGTGACA TGCAGGGCCA TTGGAATCGG AGCGTACTTG 
GTGAGACTGG GCCAAAGAAC CATACAGGTG GAAAACTCTC ATATCATCCT GACTGGAGCC 
GGAGCTCTCA ATAAGGTTCT CGGGCGTGAG GTGTACACTT CCAATAACCA ACTGGGTGGA 
GTACAGATCA TGCACAATAA CGGAGTCACA CACACCAATG TGTGCGATGA TTTTGAGGGA 
GTGTATACAT TGCTGCACTG GCTGTCTTAC ATGCCAAAGA ATATGTCCAG CCCTGTGCCG 
ATACTCTCTG CTAAAGATCC CATTGATCGA GCGATAGAGT TTGTCCCAAC CAAGGCACCT 
TATGACCCAC GCTGGATGCT AGCTGGACGT CCCAATCAGA ACATTAAGGG TGCATGGGTG 
AGTGGGTTCT TCGACCAGGG CTCTTTTCTG GAGATCATGC AGCCATGGGC ACAGAGTGTT 
GTCGTAGGAC GGGCCAGGTT GGGTGGGATC CCAACTGGAG TAGTTGCCGT GGAAACCCGA 
TCAGTGGAGC TTTCAATCCC AGCAGACCCA GCTAATCTGG ACTCGGAAGC CAAGATAATC 
CAGCAGGCTG GGCAAGTGTG GTTCCCTGAT TCTGCTTTCA AAACTGCACA AGCCATTAAA 
GACTTCAATA GAGAGGGACT TCCGCTTATA GTGTTTGCCA ACTGGAGAGG TTTTTCTGGA 
GGCATGAAAG ACATGTATGA CCAGGTGCTG AAGTTTGGAG CGTATATAGT GGACGGTCTG 
AGAGAGTACA GACAGCCGGT TCTGGTGTAT ATCCCACCAC AGGCGGAGCT CCGGGGAGGA 
TCATGGGTTG TCATAGACCC TACTATCAAT CCTCGCCACA TGGAAATGTA TGCAGACAAA 
GACAGCCGTG GCGGTGTGCT GGAGCCTGAA GGCACAGTGG AGATCAAGTT CCGCAAAAAA 
GACCTGGTGA AGACCATGCG ACGGGTGGAC CCTGTGTACA TGGGGCTTGC AGAAAGACTG 
GGAACCCCAG AGTTGAGTGT GTCTGAGCGT AAAGAGCTGG AGAGTAAACT AAAAGAGAGA 
GAAGAGTTCC TGCTGCCCAT ATATCATCAA GTAGCTGTTC AGTTCGCTGA CCTTCATGAC 
ACACCGGGCC GCATGCAGGA GAAAGGAGTC ATCACTGATA TCCTTGAGTG GAGCACGTCT 
CGGCAGTTTT TCTACTGGCG TTTGCGGAGG TTATTGCTGG AGGAAACGGT GAAGAGGAAG 
ATCCAGTGTG CTAACAGTGA GCTGACGGAC GGACAGGTTC AGGCTATGCT TCGCCGTTGG 
TTCGTGGAAG CAGAGGGAGC TGTGAAAGCG TATTTGTGGG ACAGTAATGA AGATGTGGTG 
GAGTGGTTGG AGAGGCAGCT GGCAGAGGAA GAAGGCGCCA GATCCGTCAT CGACGAGAAT 
ATCAAATATA TTCGCAGAGA TCACATCCTC AAACAGATCC GCAGCCTGGT TCAAGCTAAC 
CCTGAGGTGG CTATGGACTC CATTGTACAC ATGACCCAGC ACATTTCCCC CACACAGCGC 
GCCGAAGTCG TCCGCATCCT CTCCACCATG GACACCCCAG CCTCCTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_017356115.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211604.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X7, mRNA.

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CloneID	ODa59096
Clone ID Related Accession (Same CDS sequence)	XM_017356115.1 , XM_017356115.2
Accession Version	XM_017356115.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGAATG 
TCCTTTTCCT CAAACCTGAA CCACTACGGC ATGGTCCACG TGGCCAGCGT TAGTGATGTG 
CTCCTAGACA CCTCCTTTAC CCCACCCTGT CAGCGCATGG GAGCCATGGT TTCCTTCCGC 
TCTTTCCAGG AGTTCACCAG GAACATAAAG GATGTTCTGA GCTGTTTCTC AGACTCTCCT 
CCATCAACTC CAACCTTTCC TGAAGGGGGG AACCCTGTGC TGTATAGAGA GGAGGACAGC 
AAGAGCATTC AAGATGAGCC CATTCATATT CTTAATGTGG CAATAAAGAC GGACAGTGAT 
ATTGATGATG ATGGGCTGGC GGCCATGTTT CGTGAGTTTA CACAGTCTAA GAAATCTCTG 
CTGTTTGACC ACGGCATCCG AAGACTTACC TTCCTGGTTG CTCAGAAGAG AGAATTCCCC 
AAATTTTTTA CATTCCGGGC TCGAGACAAG TTTGAGGAAG ACCGTATCTA CAGACACTTG 
GAACCTGCAT TAGCTTTCCA GCTGGAGCTC AACCGCATGC GTAATTTTGC ACTGACAGCC 
ATCCCCTGTG CCAATCATAA GATGCACCTG TACTTGGGAG CTGCCCGTGT GGAAGTAGGC 
ACTGAGGTCA CAGATTACCG CTTCTTTGTG CGGGCAATCA TCCGTCACTC AGATTTGGTC 
ACCAAGGAAG CATCATTTGA GTACCTGCAC AACGAGGCAG AACGCTTGCT GCTGGAGGCC 
ATGGATGAAC TGGAAGTGGC TTTCAACAAC ACCACTGTGC GAACCGACTG CAATCACATC 
TTCCTCAACT TCGTTCCAAC AGTCATCATG GACCCTTCAA AGATTGAGGA ATCTGTGCGC 
TCCATGGTCA TGCGCTACGG CAGTCGCTTG TGGAAGCTCC GTGTGCTTCA AGCCGAGCTG 
AAGATCAACA TCCGATTGAC TCCCACCGGC AAACAGATCC CCATCCGCCT CTTCCTGACC 
AACGAGAGTG GCTACTACCT GGACATCAGC CTGTACAAGG AAGTCACTGA CTCCCGTACA 
GGACAGGTGG GGCACAAAGA CCGACAGATC ATGTTCCAGG CATATGGAGA TAAGCAGGGT 
CCTCTTCATG GCATGCTCAT CAACACACCC TACGTCACAA AAGACCTGCT GCAGTCCAAA 
CGCTTCCAGG CACAGAGCCT CGGCACTACC TATGTTTACG ACTTCCCAGA AATGTTTCGC 
CAGGCTTTAA GGAAGCTGTG GCAATCCATG GATGCACACG CCAACTTGCC CAAGTGCCCT 
CTTCCATCAG AACTGCTCAC CTTCACAGAG CTGGTTCTGG ATTCTCAGGG TCAGCTGGTG 
CAGATGAACC GCCTACCTGG AGGGAATGAG ATTGGCATGG TAGCATGGCG AATGACCCTC 
CGGACCCCTG AGTATCCAGC CGGCCGTGAG ATTATAGTGA TCAGCAATGA CATCACACAT 
AAGATTGGCT CGTTTGGGCC ACAAGAAGAT GTTCTGTTCC AGCAAGCTTC AGAAATGGCA 
AGAGAAAGTG GCATTCCACG GATCTATATT GCTGCCAACA GCGGAGCTCG TATAGGCCTG 
GCAGAGGAGA TCAGACACAT GTTTCATGTG GCCTGGCAGG ATCCGGTGGA CCCATACAAG 
GGTTTTAAGT ACCTATACCT CACACCACAG GACTATAAGA AGGTGTCTGC TCTGAACTCA 
GTCCACTGTG AACATGTGGA GGATGAGGGG GAATCCAGGT ATAAAATCAC TGATATCATT 
GGGAAAGAGG AAGGGTTGGG TGTGGAGAAC TTGAGAGGTT CTGGCATGAT CGCAGGTGAA 
TCGTCACTGG CGTATGAGGA TATTATCACC ATGAATCTGG TGACATGCAG GGCCATTGGA 
ATCGGAGCGT ACTTGGTGAG ACTGGGCCAA AGAACCATAC AGGTGGAAAA CTCTCATATC 
ATCCTGACTG GAGCCGGAGC TCTCAATAAG GTTCTCGGGC GTGAGGTGTA CACTTCCAAT 
AACCAACTGG GTGGAGTACA GATCATGCAC AATAACGGAG TCACACACAC CAATGTGTGC 
GATGATTTTG AGGGAGTGTA TACATTGCTG CACTGGCTGT CTTACATGCC AAAGAATATG 
TCCAGCCCTG TGCCGATACT CTCTGCTAAA GATCCCATTG ATCGAGCGAT AGAGTTTGTC 
CCAACCAAGG CACCTTATGA CCCACGCTGG ATGCTAGCTG GACGTCCCAA TCAGAACATT 
AAGGGTGCAT GGGTGAGTGG GTTCTTCGAC CAGGGCTCTT TTCTGGAGAT CATGCAGCCA 
TGGGCACAGA GTGTTGTCGT AGGACGGGCC AGGTTGGGTG GGATCCCAAC TGGAGTAGTT 
GCCGTGGAAA CCCGATCAGT GGAGCTTTCA ATCCCAGCAG ACCCAGCTAA TCTGGACTCG 
GAAGCCAAGA TAATCCAGCA GGCTGGGCAA GTGTGGTTCC CTGATTCTGC TTTCAAAACT 
GCACAAGCCA TTAAAGACTT CAATAGAGAG GGACTTCCGC TTATAGTGTT TGCCAACTGG 
AGAGGTTTTT CTGGAGGCAT GAAAGACATG TATGACCAGG TGCTGAAGTT TGGAGCGTAT 
ATAGTGGACG GTCTGAGAGA GTACAGACAG CCGGTTCTGG TGTATATCCC ACCACAGGCG 
GAGCTCCGGG GAGGATCATG GGTTGTCATA GACCCTACTA TCAATCCTCG CCACATGGAA 
ATGTATGCAG ACAAAGACAG CCGTGGCGGT GTGCTGGAGC CTGAAGGCAC AGTGGAGATC 
AAGTTCCGCA AAAAAGACCT GGTGAAGACC ATGCGACGGG TGGACCCTGT GTACATGGGG 
CTTGCAGAAA GACTGGGAAC CCCAGAGTTG AGTGTGTCTG AGCGTAAAGA GCTGGAGAGT 
AAACTAAAAG AGAGAGAAGA GTTCCTGCTG CCCATATATC ATCAAGTAGC TGTTCAGTTC 
GCTGACCTTC ATGACACACC GGGCCGCATG CAGGAGAAAG GAGTCATCAC TGATATCCTT 
GAGTGGAGCA CGTCTCGGCA GTTTTTCTAC TGGCGTTTGC GGAGGTTATT GCTGGAGGAA 
ACGGTGAAGA GGAAGATCCA GTGTGCTAAC AGTGAGCTGA CGGACGGACA GGTTCAGGCT 
ATGCTTCGCC GTTGGTTCGT GGAAGCAGAG GGAGCTGTGA AAGCGTATTT GTGGGACAGT 
AATGAAGATG TGGTGGAGTG GTTGGAGAGG CAGCTGGCAG AGGAAGAAGG CGCCAGATCC 
GTCATCGACG AGAATATCAA ATATATTCGC AGAGATCACA TCCTCAAACA GATCCGCAGC 
CTGGTTCAAG CTAACCCTGA GGTGGCTATG GACTCCATTG TACACATGAC CCAGCACATT 
TCCCCCACAC AGCGCGCCGA AGTCGTCCGC ATCCTCTCCA CCATGGACAC CCCAGCCTCC 
TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211604.1
CDS	242..7267
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_017356115.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356115.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGAATG 
TCCTTTTCCT CAAACCTGAA CCACTACGGC ATGGTCCACG TGGCCAGCGT TAGTGATGTG 
CTCCTAGACA CCTCCTTTAC CCCACCCTGT CAGCGCATGG GAGCCATGGT TTCCTTCCGC 
TCTTTCCAGG AGTTCACCAG GAACATAAAG GATGTTCTGA GCTGTTTCTC AGACTCTCCT 
CCATCAACTC CAACCTTTCC TGAAGGGGGG AACCCTGTGC TGTATAGAGA GGAGGACAGC 
AAGAGCATTC AAGATGAGCC CATTCATATT CTTAATGTGG CAATAAAGAC GGACAGTGAT 
ATTGATGATG ATGGGCTGGC GGCCATGTTT CGTGAGTTTA CACAGTCTAA GAAATCTCTG 
CTGTTTGACC ACGGCATCCG AAGACTTACC TTCCTGGTTG CTCAGAAGAG AGAATTCCCC 
AAATTTTTTA CATTCCGGGC TCGAGACAAG TTTGAGGAAG ACCGTATCTA CAGACACTTG 
GAACCTGCAT TAGCTTTCCA GCTGGAGCTC AACCGCATGC GTAATTTTGC ACTGACAGCC 
ATCCCCTGTG CCAATCATAA GATGCACCTG TACTTGGGAG CTGCCCGTGT GGAAGTAGGC 
ACTGAGGTCA CAGATTACCG CTTCTTTGTG CGGGCAATCA TCCGTCACTC AGATTTGGTC 
ACCAAGGAAG CATCATTTGA GTACCTGCAC AACGAGGCAG AACGCTTGCT GCTGGAGGCC 
ATGGATGAAC TGGAAGTGGC TTTCAACAAC ACCACTGTGC GAACCGACTG CAATCACATC 
TTCCTCAACT TCGTTCCAAC AGTCATCATG GACCCTTCAA AGATTGAGGA ATCTGTGCGC 
TCCATGGTCA TGCGCTACGG CAGTCGCTTG TGGAAGCTCC GTGTGCTTCA AGCCGAGCTG 
AAGATCAACA TCCGATTGAC TCCCACCGGC AAACAGATCC CCATCCGCCT CTTCCTGACC 
AACGAGAGTG GCTACTACCT GGACATCAGC CTGTACAAGG AAGTCACTGA CTCCCGTACA 
GGACAGGTGG GGCACAAAGA CCGACAGATC ATGTTCCAGG CATATGGAGA TAAGCAGGGT 
CCTCTTCATG GCATGCTCAT CAACACACCC TACGTCACAA AAGACCTGCT GCAGTCCAAA 
CGCTTCCAGG CACAGAGCCT CGGCACTACC TATGTTTACG ACTTCCCAGA AATGTTTCGC 
CAGGCTTTAA GGAAGCTGTG GCAATCCATG GATGCACACG CCAACTTGCC CAAGTGCCCT 
CTTCCATCAG AACTGCTCAC CTTCACAGAG CTGGTTCTGG ATTCTCAGGG TCAGCTGGTG 
CAGATGAACC GCCTACCTGG AGGGAATGAG ATTGGCATGG TAGCATGGCG AATGACCCTC 
CGGACCCCTG AGTATCCAGC CGGCCGTGAG ATTATAGTGA TCAGCAATGA CATCACACAT 
AAGATTGGCT CGTTTGGGCC ACAAGAAGAT GTTCTGTTCC AGCAAGCTTC AGAAATGGCA 
AGAGAAAGTG GCATTCCACG GATCTATATT GCTGCCAACA GCGGAGCTCG TATAGGCCTG 
GCAGAGGAGA TCAGACACAT GTTTCATGTG GCCTGGCAGG ATCCGGTGGA CCCATACAAG 
GGTTTTAAGT ACCTATACCT CACACCACAG GACTATAAGA AGGTGTCTGC TCTGAACTCA 
GTCCACTGTG AACATGTGGA GGATGAGGGG GAATCCAGGT ATAAAATCAC TGATATCATT 
GGGAAAGAGG AAGGGTTGGG TGTGGAGAAC TTGAGAGGTT CTGGCATGAT CGCAGGTGAA 
TCGTCACTGG CGTATGAGGA TATTATCACC ATGAATCTGG TGACATGCAG GGCCATTGGA 
ATCGGAGCGT ACTTGGTGAG ACTGGGCCAA AGAACCATAC AGGTGGAAAA CTCTCATATC 
ATCCTGACTG GAGCCGGAGC TCTCAATAAG GTTCTCGGGC GTGAGGTGTA CACTTCCAAT 
AACCAACTGG GTGGAGTACA GATCATGCAC AATAACGGAG TCACACACAC CAATGTGTGC 
GATGATTTTG AGGGAGTGTA TACATTGCTG CACTGGCTGT CTTACATGCC AAAGAATATG 
TCCAGCCCTG TGCCGATACT CTCTGCTAAA GATCCCATTG ATCGAGCGAT AGAGTTTGTC 
CCAACCAAGG CACCTTATGA CCCACGCTGG ATGCTAGCTG GACGTCCCAA TCAGAACATT 
AAGGGTGCAT GGGTGAGTGG GTTCTTCGAC CAGGGCTCTT TTCTGGAGAT CATGCAGCCA 
TGGGCACAGA GTGTTGTCGT AGGACGGGCC AGGTTGGGTG GGATCCCAAC TGGAGTAGTT 
GCCGTGGAAA CCCGATCAGT GGAGCTTTCA ATCCCAGCAG ACCCAGCTAA TCTGGACTCG 
GAAGCCAAGA TAATCCAGCA GGCTGGGCAA GTGTGGTTCC CTGATTCTGC TTTCAAAACT 
GCACAAGCCA TTAAAGACTT CAATAGAGAG GGACTTCCGC TTATAGTGTT TGCCAACTGG 
AGAGGTTTTT CTGGAGGCAT GAAAGACATG TATGACCAGG TGCTGAAGTT TGGAGCGTAT 
ATAGTGGACG GTCTGAGAGA GTACAGACAG CCGGTTCTGG TGTATATCCC ACCACAGGCG 
GAGCTCCGGG GAGGATCATG GGTTGTCATA GACCCTACTA TCAATCCTCG CCACATGGAA 
ATGTATGCAG ACAAAGACAG CCGTGGCGGT GTGCTGGAGC CTGAAGGCAC AGTGGAGATC 
AAGTTCCGCA AAAAAGACCT GGTGAAGACC ATGCGACGGG TGGACCCTGT GTACATGGGG 
CTTGCAGAAA GACTGGGAAC CCCAGAGTTG AGTGTGTCTG AGCGTAAAGA GCTGGAGAGT 
AAACTAAAAG AGAGAGAAGA GTTCCTGCTG CCCATATATC ATCAAGTAGC TGTTCAGTTC 
GCTGACCTTC ATGACACACC GGGCCGCATG CAGGAGAAAG GAGTCATCAC TGATATCCTT 
GAGTGGAGCA CGTCTCGGCA GTTTTTCTAC TGGCGTTTGC GGAGGTTATT GCTGGAGGAA 
ACGGTGAAGA GGAAGATCCA GTGTGCTAAC AGTGAGCTGA CGGACGGACA GGTTCAGGCT 
ATGCTTCGCC GTTGGTTCGT GGAAGCAGAG GGAGCTGTGA AAGCGTATTT GTGGGACAGT 
AATGAAGATG TGGTGGAGTG GTTGGAGAGG CAGCTGGCAG AGGAAGAAGG CGCCAGATCC 
GTCATCGACG AGAATATCAA ATATATTCGC AGAGATCACA TCCTCAAACA GATCCGCAGC 
CTGGTTCAAG CTAACCCTGA GGTGGCTATG GACTCCATTG TACACATGAC CCAGCACATT 
TCCCCCACAC AGCGCGCCGA AGTCGTCCGC ATCCTCTCCA CCATGGACAC CCCAGCCTCC 
TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_017356115.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211604.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X14, mRNA.

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CloneID	ODa59096
Clone ID Related Accession (Same CDS sequence)	XM_017356115.1 , XM_017356115.2
Accession Version	XM_017356115.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356115.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGAATG 
TCCTTTTCCT CAAACCTGAA CCACTACGGC ATGGTCCACG TGGCCAGCGT TAGTGATGTG 
CTCCTAGACA CCTCCTTTAC CCCACCCTGT CAGCGCATGG GAGCCATGGT TTCCTTCCGC 
TCTTTCCAGG AGTTCACCAG GAACATAAAG GATGTTCTGA GCTGTTTCTC AGACTCTCCT 
CCATCAACTC CAACCTTTCC TGAAGGGGGG AACCCTGTGC TGTATAGAGA GGAGGACAGC 
AAGAGCATTC AAGATGAGCC CATTCATATT CTTAATGTGG CAATAAAGAC GGACAGTGAT 
ATTGATGATG ATGGGCTGGC GGCCATGTTT CGTGAGTTTA CACAGTCTAA GAAATCTCTG 
CTGTTTGACC ACGGCATCCG AAGACTTACC TTCCTGGTTG CTCAGAAGAG AGAATTCCCC 
AAATTTTTTA CATTCCGGGC TCGAGACAAG TTTGAGGAAG ACCGTATCTA CAGACACTTG 
GAACCTGCAT TAGCTTTCCA GCTGGAGCTC AACCGCATGC GTAATTTTGC ACTGACAGCC 
ATCCCCTGTG CCAATCATAA GATGCACCTG TACTTGGGAG CTGCCCGTGT GGAAGTAGGC 
ACTGAGGTCA CAGATTACCG CTTCTTTGTG CGGGCAATCA TCCGTCACTC AGATTTGGTC 
ACCAAGGAAG CATCATTTGA GTACCTGCAC AACGAGGCAG AACGCTTGCT GCTGGAGGCC 
ATGGATGAAC TGGAAGTGGC TTTCAACAAC ACCACTGTGC GAACCGACTG CAATCACATC 
TTCCTCAACT TCGTTCCAAC AGTCATCATG GACCCTTCAA AGATTGAGGA ATCTGTGCGC 
TCCATGGTCA TGCGCTACGG CAGTCGCTTG TGGAAGCTCC GTGTGCTTCA AGCCGAGCTG 
AAGATCAACA TCCGATTGAC TCCCACCGGC AAACAGATCC CCATCCGCCT CTTCCTGACC 
AACGAGAGTG GCTACTACCT GGACATCAGC CTGTACAAGG AAGTCACTGA CTCCCGTACA 
GGACAGGTGG GGCACAAAGA CCGACAGATC ATGTTCCAGG CATATGGAGA TAAGCAGGGT 
CCTCTTCATG GCATGCTCAT CAACACACCC TACGTCACAA AAGACCTGCT GCAGTCCAAA 
CGCTTCCAGG CACAGAGCCT CGGCACTACC TATGTTTACG ACTTCCCAGA AATGTTTCGC 
CAGGCTTTAA GGAAGCTGTG GCAATCCATG GATGCACACG CCAACTTGCC CAAGTGCCCT 
CTTCCATCAG AACTGCTCAC CTTCACAGAG CTGGTTCTGG ATTCTCAGGG TCAGCTGGTG 
CAGATGAACC GCCTACCTGG AGGGAATGAG ATTGGCATGG TAGCATGGCG AATGACCCTC 
CGGACCCCTG AGTATCCAGC CGGCCGTGAG ATTATAGTGA TCAGCAATGA CATCACACAT 
AAGATTGGCT CGTTTGGGCC ACAAGAAGAT GTTCTGTTCC AGCAAGCTTC AGAAATGGCA 
AGAGAAAGTG GCATTCCACG GATCTATATT GCTGCCAACA GCGGAGCTCG TATAGGCCTG 
GCAGAGGAGA TCAGACACAT GTTTCATGTG GCCTGGCAGG ATCCGGTGGA CCCATACAAG 
GGTTTTAAGT ACCTATACCT CACACCACAG GACTATAAGA AGGTGTCTGC TCTGAACTCA 
GTCCACTGTG AACATGTGGA GGATGAGGGG GAATCCAGGT ATAAAATCAC TGATATCATT 
GGGAAAGAGG AAGGGTTGGG TGTGGAGAAC TTGAGAGGTT CTGGCATGAT CGCAGGTGAA 
TCGTCACTGG CGTATGAGGA TATTATCACC ATGAATCTGG TGACATGCAG GGCCATTGGA 
ATCGGAGCGT ACTTGGTGAG ACTGGGCCAA AGAACCATAC AGGTGGAAAA CTCTCATATC 
ATCCTGACTG GAGCCGGAGC TCTCAATAAG GTTCTCGGGC GTGAGGTGTA CACTTCCAAT 
AACCAACTGG GTGGAGTACA GATCATGCAC AATAACGGAG TCACACACAC CAATGTGTGC 
GATGATTTTG AGGGAGTGTA TACATTGCTG CACTGGCTGT CTTACATGCC AAAGAATATG 
TCCAGCCCTG TGCCGATACT CTCTGCTAAA GATCCCATTG ATCGAGCGAT AGAGTTTGTC 
CCAACCAAGG CACCTTATGA CCCACGCTGG ATGCTAGCTG GACGTCCCAA TCAGAACATT 
AAGGGTGCAT GGGTGAGTGG GTTCTTCGAC CAGGGCTCTT TTCTGGAGAT CATGCAGCCA 
TGGGCACAGA GTGTTGTCGT AGGACGGGCC AGGTTGGGTG GGATCCCAAC TGGAGTAGTT 
GCCGTGGAAA CCCGATCAGT GGAGCTTTCA ATCCCAGCAG ACCCAGCTAA TCTGGACTCG 
GAAGCCAAGA TAATCCAGCA GGCTGGGCAA GTGTGGTTCC CTGATTCTGC TTTCAAAACT 
GCACAAGCCA TTAAAGACTT CAATAGAGAG GGACTTCCGC TTATAGTGTT TGCCAACTGG 
AGAGGTTTTT CTGGAGGCAT GAAAGACATG TATGACCAGG TGCTGAAGTT TGGAGCGTAT 
ATAGTGGACG GTCTGAGAGA GTACAGACAG CCGGTTCTGG TGTATATCCC ACCACAGGCG 
GAGCTCCGGG GAGGATCATG GGTTGTCATA GACCCTACTA TCAATCCTCG CCACATGGAA 
ATGTATGCAG ACAAAGACAG CCGTGGCGGT GTGCTGGAGC CTGAAGGCAC AGTGGAGATC 
AAGTTCCGCA AAAAAGACCT GGTGAAGACC ATGCGACGGG TGGACCCTGT GTACATGGGG 
CTTGCAGAAA GACTGGGAAC CCCAGAGTTG AGTGTGTCTG AGCGTAAAGA GCTGGAGAGT 
AAACTAAAAG AGAGAGAAGA GTTCCTGCTG CCCATATATC ATCAAGTAGC TGTTCAGTTC 
GCTGACCTTC ATGACACACC GGGCCGCATG CAGGAGAAAG GAGTCATCAC TGATATCCTT 
GAGTGGAGCA CGTCTCGGCA GTTTTTCTAC TGGCGTTTGC GGAGGTTATT GCTGGAGGAA 
ACGGTGAAGA GGAAGATCCA GTGTGCTAAC AGTGAGCTGA CGGACGGACA GGTTCAGGCT 
ATGCTTCGCC GTTGGTTCGT GGAAGCAGAG GGAGCTGTGA AAGCGTATTT GTGGGACAGT 
AATGAAGATG TGGTGGAGTG GTTGGAGAGG CAGCTGGCAG AGGAAGAAGG CGCCAGATCC 
GTCATCGACG AGAATATCAA ATATATTCGC AGAGATCACA TCCTCAAACA GATCCGCAGC 
CTGGTTCAAG CTAACCCTGA GGTGGCTATG GACTCCATTG TACACATGAC CCAGCACATT 
TCCCCCACAC AGCGCGCCGA AGTCGTCCGC ATCCTCTCCA CCATGGACAC CCCAGCCTCC 
TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211604.1
CDS	236..7261
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_017356115.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356115.2

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGAATG 
TCCTTTTCCT CAAACCTGAA CCACTACGGC ATGGTCCACG TGGCCAGCGT TAGTGATGTG 
CTCCTAGACA CCTCCTTTAC CCCACCCTGT CAGCGCATGG GAGCCATGGT TTCCTTCCGC 
TCTTTCCAGG AGTTCACCAG GAACATAAAG GATGTTCTGA GCTGTTTCTC AGACTCTCCT 
CCATCAACTC CAACCTTTCC TGAAGGGGGG AACCCTGTGC TGTATAGAGA GGAGGACAGC 
AAGAGCATTC AAGATGAGCC CATTCATATT CTTAATGTGG CAATAAAGAC GGACAGTGAT 
ATTGATGATG ATGGGCTGGC GGCCATGTTT CGTGAGTTTA CACAGTCTAA GAAATCTCTG 
CTGTTTGACC ACGGCATCCG AAGACTTACC TTCCTGGTTG CTCAGAAGAG AGAATTCCCC 
AAATTTTTTA CATTCCGGGC TCGAGACAAG TTTGAGGAAG ACCGTATCTA CAGACACTTG 
GAACCTGCAT TAGCTTTCCA GCTGGAGCTC AACCGCATGC GTAATTTTGC ACTGACAGCC 
ATCCCCTGTG CCAATCATAA GATGCACCTG TACTTGGGAG CTGCCCGTGT GGAAGTAGGC 
ACTGAGGTCA CAGATTACCG CTTCTTTGTG CGGGCAATCA TCCGTCACTC AGATTTGGTC 
ACCAAGGAAG CATCATTTGA GTACCTGCAC AACGAGGCAG AACGCTTGCT GCTGGAGGCC 
ATGGATGAAC TGGAAGTGGC TTTCAACAAC ACCACTGTGC GAACCGACTG CAATCACATC 
TTCCTCAACT TCGTTCCAAC AGTCATCATG GACCCTTCAA AGATTGAGGA ATCTGTGCGC 
TCCATGGTCA TGCGCTACGG CAGTCGCTTG TGGAAGCTCC GTGTGCTTCA AGCCGAGCTG 
AAGATCAACA TCCGATTGAC TCCCACCGGC AAACAGATCC CCATCCGCCT CTTCCTGACC 
AACGAGAGTG GCTACTACCT GGACATCAGC CTGTACAAGG AAGTCACTGA CTCCCGTACA 
GGACAGGTGG GGCACAAAGA CCGACAGATC ATGTTCCAGG CATATGGAGA TAAGCAGGGT 
CCTCTTCATG GCATGCTCAT CAACACACCC TACGTCACAA AAGACCTGCT GCAGTCCAAA 
CGCTTCCAGG CACAGAGCCT CGGCACTACC TATGTTTACG ACTTCCCAGA AATGTTTCGC 
CAGGCTTTAA GGAAGCTGTG GCAATCCATG GATGCACACG CCAACTTGCC CAAGTGCCCT 
CTTCCATCAG AACTGCTCAC CTTCACAGAG CTGGTTCTGG ATTCTCAGGG TCAGCTGGTG 
CAGATGAACC GCCTACCTGG AGGGAATGAG ATTGGCATGG TAGCATGGCG AATGACCCTC 
CGGACCCCTG AGTATCCAGC CGGCCGTGAG ATTATAGTGA TCAGCAATGA CATCACACAT 
AAGATTGGCT CGTTTGGGCC ACAAGAAGAT GTTCTGTTCC AGCAAGCTTC AGAAATGGCA 
AGAGAAAGTG GCATTCCACG GATCTATATT GCTGCCAACA GCGGAGCTCG TATAGGCCTG 
GCAGAGGAGA TCAGACACAT GTTTCATGTG GCCTGGCAGG ATCCGGTGGA CCCATACAAG 
GGTTTTAAGT ACCTATACCT CACACCACAG GACTATAAGA AGGTGTCTGC TCTGAACTCA 
GTCCACTGTG AACATGTGGA GGATGAGGGG GAATCCAGGT ATAAAATCAC TGATATCATT 
GGGAAAGAGG AAGGGTTGGG TGTGGAGAAC TTGAGAGGTT CTGGCATGAT CGCAGGTGAA 
TCGTCACTGG CGTATGAGGA TATTATCACC ATGAATCTGG TGACATGCAG GGCCATTGGA 
ATCGGAGCGT ACTTGGTGAG ACTGGGCCAA AGAACCATAC AGGTGGAAAA CTCTCATATC 
ATCCTGACTG GAGCCGGAGC TCTCAATAAG GTTCTCGGGC GTGAGGTGTA CACTTCCAAT 
AACCAACTGG GTGGAGTACA GATCATGCAC AATAACGGAG TCACACACAC CAATGTGTGC 
GATGATTTTG AGGGAGTGTA TACATTGCTG CACTGGCTGT CTTACATGCC AAAGAATATG 
TCCAGCCCTG TGCCGATACT CTCTGCTAAA GATCCCATTG ATCGAGCGAT AGAGTTTGTC 
CCAACCAAGG CACCTTATGA CCCACGCTGG ATGCTAGCTG GACGTCCCAA TCAGAACATT 
AAGGGTGCAT GGGTGAGTGG GTTCTTCGAC CAGGGCTCTT TTCTGGAGAT CATGCAGCCA 
TGGGCACAGA GTGTTGTCGT AGGACGGGCC AGGTTGGGTG GGATCCCAAC TGGAGTAGTT 
GCCGTGGAAA CCCGATCAGT GGAGCTTTCA ATCCCAGCAG ACCCAGCTAA TCTGGACTCG 
GAAGCCAAGA TAATCCAGCA GGCTGGGCAA GTGTGGTTCC CTGATTCTGC TTTCAAAACT 
GCACAAGCCA TTAAAGACTT CAATAGAGAG GGACTTCCGC TTATAGTGTT TGCCAACTGG 
AGAGGTTTTT CTGGAGGCAT GAAAGACATG TATGACCAGG TGCTGAAGTT TGGAGCGTAT 
ATAGTGGACG GTCTGAGAGA GTACAGACAG CCGGTTCTGG TGTATATCCC ACCACAGGCG 
GAGCTCCGGG GAGGATCATG GGTTGTCATA GACCCTACTA TCAATCCTCG CCACATGGAA 
ATGTATGCAG ACAAAGACAG CCGTGGCGGT GTGCTGGAGC CTGAAGGCAC AGTGGAGATC 
AAGTTCCGCA AAAAAGACCT GGTGAAGACC ATGCGACGGG TGGACCCTGT GTACATGGGG 
CTTGCAGAAA GACTGGGAAC CCCAGAGTTG AGTGTGTCTG AGCGTAAAGA GCTGGAGAGT 
AAACTAAAAG AGAGAGAAGA GTTCCTGCTG CCCATATATC ATCAAGTAGC TGTTCAGTTC 
GCTGACCTTC ATGACACACC GGGCCGCATG CAGGAGAAAG GAGTCATCAC TGATATCCTT 
GAGTGGAGCA CGTCTCGGCA GTTTTTCTAC TGGCGTTTGC GGAGGTTATT GCTGGAGGAA 
ACGGTGAAGA GGAAGATCCA GTGTGCTAAC AGTGAGCTGA CGGACGGACA GGTTCAGGCT 
ATGCTTCGCC GTTGGTTCGT GGAAGCAGAG GGAGCTGTGA AAGCGTATTT GTGGGACAGT 
AATGAAGATG TGGTGGAGTG GTTGGAGAGG CAGCTGGCAG AGGAAGAAGG CGCCAGATCC 
GTCATCGACG AGAATATCAA ATATATTCGC AGAGATCACA TCCTCAAACA GATCCGCAGC 
CTGGTTCAAG CTAACCCTGA GGTGGCTATG GACTCCATTG TACACATGAC CCAGCACATT 
TCCCCCACAC AGCGCGCCGA AGTCGTCCGC ATCCTCTCCA CCATGGACAC CCCAGCCTCC 
TCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_017356117.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211606.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X15, mRNA.

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CloneID	ODa59098
Clone ID Related Accession (Same CDS sequence)	XM_017356117.2 , XM_017356117.1
Accession Version	XM_017356117.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356117.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGAATG 
TCCTTTTCCT CAAACCTGAA CCACTACGGC ATGGTCCACG TGGCCAGCGT TAGTGATGTG 
CTCCTAGACA CCTCCTTTAC CCCACCCTGT CAGCGCATGG GAGCCATGGT TTCCTTCCGC 
TCTTTCCAGG AGTTCACCAG GAACATAAAG GATGTTCTGA GCTGTTTCTC AGACTCTCCT 
CCATCAACTC CAACCTTTCC TGAAGGGGGG AACCCTGTGC TGTATAGAGA GGAGGACAGC 
AAGAGCATTC AAGATGAGCC CATTCATATT CTTAATGTGG CAATAAAGAC GGACAGTGAT 
ATTGATGATG ATGGGCTGGC GGCCATGTTT CGTGAGTTTA CACAGTCTAA GAAATCTCTG 
CTGTTTGACC ACGGCATCCG AAGACTTACC TTCCTGGTTG CTCAGAAGGA TTTCAGAAAG 
CAAATCAACT GTGAGGTGGA CCAGAGGTTC CATAGAGAAT TCCCCAAATT TTTTACATTC 
CGGGCTCGAG ACAAGTTTGA GGAAGACCGT ATCTACAGAC ACTTGGAACC TGCATTAGCT 
TTCCAGCTGG AGCTCAACCG CATGCGTAAT TTTGCACTGA CAGCCATCCC CTGTGCCAAT 
CATAAGATGC ACCTGTACTT GGGAGCTGCC CGTGTGGAAG TAGGCACTGA GGTCACAGAT 
TACCGCTTCT TTGTGCGGGC AATCATCCGT CACTCAGATT TGGTCACCAA GGAAGCATCA 
TTTGAGTACC TGCACAACGA GGCAGAACGC TTGCTGCTGG AGGCCATGGA TGAACTGGAA 
GTGGCTTTCA ACAACACCAC TGTGCGAACC GACTGCAATC ACATCTTCCT CAACTTCGTT 
CCAACAGTCA TCATGGACCC TTCAAAGATT GAGGAATCTG TGCGCTCCAT GGTCATGCGC 
TACGGCAGTC GCTTGTGGAA GCTCCGTGTG CTTCAAGCCG AGCTGAAGAT CAACATCCGA 
TTGACTCCCA CCGGCAAACA GATCCCCATC CGCCTCTTCC TGACCAACGA GAGTGGCTAC 
TACCTGGACA TCAGCCTGTA CAAGGAAGTC ACTGACTCCC GTACAGGACA GATCATGTTC 
CAGGCATATG GAGATAAGCA GGGTCCTCTT CATGGCATGC TCATCAACAC ACCCTACGTC 
ACAAAAGACC TGCTGCAGTC CAAACGCTTC CAGGCACAGA GCCTCGGCAC TACCTATGTT 
TACGACTTCC CAGAAATGTT TCGCCAGGCT TTAAGGAAGC TGTGGCAATC CATGGATGCA 
CACGCCAACT TGCCCAAGTG CCCTCTTCCA TCAGAACTGC TCACCTTCAC AGAGCTGGTT 
CTGGATTCTC AGGGTCAGCT GGTGCAGATG AACCGCCTAC CTGGAGGGAA TGAGATTGGC 
ATGGTAGCAT GGCGAATGAC CCTCCGGACC CCTGAGTATC CAGCCGGCCG TGAGATTATA 
GTGATCAGCA ATGACATCAC ACATAAGATT GGCTCGTTTG GGCCACAAGA AGATGTTCTG 
TTCCAGCAAG CTTCAGAAAT GGCAAGAGAA AGTGGCATTC CACGGATCTA TATTGCTGCC 
AACAGCGGAG CTCGTATAGG CCTGGCAGAG GAGATCAGAC ACATGTTTCA TGTGGCCTGG 
CAGGATCCGG TGGACCCATA CAAGGGTTTT AAGTACCTAT ACCTCACACC ACAGGACTAT 
AAGAAGGTGT CTGCTCTGAA CTCAGTCCAC TGTGAACATG TGGAGGATGA GGGGGAATCC 
AGGTATAAAA TCACTGATAT CATTGGGAAA GAGGAAGGGT TGGGTGTGGA GAACTTGAGA 
GGTTCTGGCA TGATCGCAGG TGAATCGTCA CTGGCGTATG AGGATATTAT CACCATGAAT 
CTGGTGACAT GCAGGGCCAT TGGAATCGGA GCGTACTTGG TGAGACTGGG CCAAAGAACC 
ATACAGGTGG AAAACTCTCA TATCATCCTG ACTGGAGCCG GAGCTCTCAA TAAGGTTCTC 
GGGCGTGAGG TGTACACTTC CAATAACCAA CTGGGTGGAG TACAGATCAT GCACAATAAC 
GGAGTCACAC ACACCAATGT GTGCGATGAT TTTGAGGGAG TGTATACATT GCTGCACTGG 
CTGTCTTACA TGCCAAAGAA TATGTCCAGC CCTGTGCCGA TACTCTCTGC TAAAGATCCC 
ATTGATCGAG CGATAGAGTT TGTCCCAACC AAGGCACCTT ATGACCCACG CTGGATGCTA 
GCTGGACGTC CCAATCAGAA CATTAAGGGT GCATGGGTGA GTGGGTTCTT CGACCAGGGC 
TCTTTTCTGG AGATCATGCA GCCATGGGCA CAGAGTGTTG TCGTAGGACG GGCCAGGTTG 
GGTGGGATCC CAACTGGAGT AGTTGCCGTG GAAACCCGAT CAGTGGAGCT TTCAATCCCA 
GCAGACCCAG CTAATCTGGA CTCGGAAGCC AAGATAATCC AGCAGGCTGG GCAAGTGTGG 
TTCCCTGATT CTGCTTTCAA AACTGCACAA GCCATTAAAG ACTTCAATAG AGAGGGACTT 
CCGCTTATAG TGTTTGCCAA CTGGAGAGGT TTTTCTGGAG GCATGAAAGA CATGTATGAC 
CAGGTGCTGA AGTTTGGAGC GTATATAGTG GACGGTCTGA GAGAGTACAG ACAGCCGGTT 
CTGGTGTATA TCCCACCACA GGCGGAGCTC CGGGGAGGAT CATGGGTTGT CATAGACCCT 
ACTATCAATC CTCGCCACAT GGAAATGTAT GCAGACAAAG ACAGCCGTGG CGGTGTGCTG 
GAGCCTGAAG GCACAGTGGA GATCAAGTTC CGCAAAAAAG ACCTGGTGAA GACCATGCGA 
CGGGTGGACC CTGTGTACAT GGGGCTTGCA GAAAGACTGG GAACCCCAGA GTTGAGTGTG 
TCTGAGCGTA AAGAGCTGGA GAGTAAACTA AAAGAGAGAG AAGAGTTCCT GCTGCCCATA 
TATCATCAAG TAGCTGTTCA GTTCGCTGAC CTTCATGACA CACCGGGCCG CATGCAGGAG 
AAAGGAGTCA TCACTGATAT CCTTGAGTGG AGCACGTCTC GGCAGTTTTT CTACTGGCGT 
TTGCGGAGGT TATTGCTGGA GGAAACGGTG AAGAGGAAGA TCCAGTGTGC TAACAGTGAG 
CTGACGGACG GACAGGTTCA GGCTATGCTT CGCCGTTGGT TCGTGGAAGC AGAGGGAGCT 
GTGAAAGCGT ATTTGTGGGA CAGTAATGAA GATGTGGTGG AGTGGTTGGA GAGGCAGCTG 
GCAGAGGAAG AAGGCGCCAG ATCCGTCATC GACGAGAATA TCAAATATAT TCGCAGAGAT 
CACATCCTCA AACAGATCCG CAGCCTGGTT CAAGCTAACC CTGAGGTGGC TATGGACTCC 
ATTGTACACA TGACCCAGCA CATTTCCCCC ACACAGCGCG CCGAAGTCGT CCGCATCCTC 
TCCACCATGG ACACCCCAGC CTCCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211606.1
CDS	237..7286
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_017356117.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356117.2

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGAATG 
TCCTTTTCCT CAAACCTGAA CCACTACGGC ATGGTCCACG TGGCCAGCGT TAGTGATGTG 
CTCCTAGACA CCTCCTTTAC CCCACCCTGT CAGCGCATGG GAGCCATGGT TTCCTTCCGC 
TCTTTCCAGG AGTTCACCAG GAACATAAAG GATGTTCTGA GCTGTTTCTC AGACTCTCCT 
CCATCAACTC CAACCTTTCC TGAAGGGGGG AACCCTGTGC TGTATAGAGA GGAGGACAGC 
AAGAGCATTC AAGATGAGCC CATTCATATT CTTAATGTGG CAATAAAGAC GGACAGTGAT 
ATTGATGATG ATGGGCTGGC GGCCATGTTT CGTGAGTTTA CACAGTCTAA GAAATCTCTG 
CTGTTTGACC ACGGCATCCG AAGACTTACC TTCCTGGTTG CTCAGAAGGA TTTCAGAAAG 
CAAATCAACT GTGAGGTGGA CCAGAGGTTC CATAGAGAAT TCCCCAAATT TTTTACATTC 
CGGGCTCGAG ACAAGTTTGA GGAAGACCGT ATCTACAGAC ACTTGGAACC TGCATTAGCT 
TTCCAGCTGG AGCTCAACCG CATGCGTAAT TTTGCACTGA CAGCCATCCC CTGTGCCAAT 
CATAAGATGC ACCTGTACTT GGGAGCTGCC CGTGTGGAAG TAGGCACTGA GGTCACAGAT 
TACCGCTTCT TTGTGCGGGC AATCATCCGT CACTCAGATT TGGTCACCAA GGAAGCATCA 
TTTGAGTACC TGCACAACGA GGCAGAACGC TTGCTGCTGG AGGCCATGGA TGAACTGGAA 
GTGGCTTTCA ACAACACCAC TGTGCGAACC GACTGCAATC ACATCTTCCT CAACTTCGTT 
CCAACAGTCA TCATGGACCC TTCAAAGATT GAGGAATCTG TGCGCTCCAT GGTCATGCGC 
TACGGCAGTC GCTTGTGGAA GCTCCGTGTG CTTCAAGCCG AGCTGAAGAT CAACATCCGA 
TTGACTCCCA CCGGCAAACA GATCCCCATC CGCCTCTTCC TGACCAACGA GAGTGGCTAC 
TACCTGGACA TCAGCCTGTA CAAGGAAGTC ACTGACTCCC GTACAGGACA GATCATGTTC 
CAGGCATATG GAGATAAGCA GGGTCCTCTT CATGGCATGC TCATCAACAC ACCCTACGTC 
ACAAAAGACC TGCTGCAGTC CAAACGCTTC CAGGCACAGA GCCTCGGCAC TACCTATGTT 
TACGACTTCC CAGAAATGTT TCGCCAGGCT TTAAGGAAGC TGTGGCAATC CATGGATGCA 
CACGCCAACT TGCCCAAGTG CCCTCTTCCA TCAGAACTGC TCACCTTCAC AGAGCTGGTT 
CTGGATTCTC AGGGTCAGCT GGTGCAGATG AACCGCCTAC CTGGAGGGAA TGAGATTGGC 
ATGGTAGCAT GGCGAATGAC CCTCCGGACC CCTGAGTATC CAGCCGGCCG TGAGATTATA 
GTGATCAGCA ATGACATCAC ACATAAGATT GGCTCGTTTG GGCCACAAGA AGATGTTCTG 
TTCCAGCAAG CTTCAGAAAT GGCAAGAGAA AGTGGCATTC CACGGATCTA TATTGCTGCC 
AACAGCGGAG CTCGTATAGG CCTGGCAGAG GAGATCAGAC ACATGTTTCA TGTGGCCTGG 
CAGGATCCGG TGGACCCATA CAAGGGTTTT AAGTACCTAT ACCTCACACC ACAGGACTAT 
AAGAAGGTGT CTGCTCTGAA CTCAGTCCAC TGTGAACATG TGGAGGATGA GGGGGAATCC 
AGGTATAAAA TCACTGATAT CATTGGGAAA GAGGAAGGGT TGGGTGTGGA GAACTTGAGA 
GGTTCTGGCA TGATCGCAGG TGAATCGTCA CTGGCGTATG AGGATATTAT CACCATGAAT 
CTGGTGACAT GCAGGGCCAT TGGAATCGGA GCGTACTTGG TGAGACTGGG CCAAAGAACC 
ATACAGGTGG AAAACTCTCA TATCATCCTG ACTGGAGCCG GAGCTCTCAA TAAGGTTCTC 
GGGCGTGAGG TGTACACTTC CAATAACCAA CTGGGTGGAG TACAGATCAT GCACAATAAC 
GGAGTCACAC ACACCAATGT GTGCGATGAT TTTGAGGGAG TGTATACATT GCTGCACTGG 
CTGTCTTACA TGCCAAAGAA TATGTCCAGC CCTGTGCCGA TACTCTCTGC TAAAGATCCC 
ATTGATCGAG CGATAGAGTT TGTCCCAACC AAGGCACCTT ATGACCCACG CTGGATGCTA 
GCTGGACGTC CCAATCAGAA CATTAAGGGT GCATGGGTGA GTGGGTTCTT CGACCAGGGC 
TCTTTTCTGG AGATCATGCA GCCATGGGCA CAGAGTGTTG TCGTAGGACG GGCCAGGTTG 
GGTGGGATCC CAACTGGAGT AGTTGCCGTG GAAACCCGAT CAGTGGAGCT TTCAATCCCA 
GCAGACCCAG CTAATCTGGA CTCGGAAGCC AAGATAATCC AGCAGGCTGG GCAAGTGTGG 
TTCCCTGATT CTGCTTTCAA AACTGCACAA GCCATTAAAG ACTTCAATAG AGAGGGACTT 
CCGCTTATAG TGTTTGCCAA CTGGAGAGGT TTTTCTGGAG GCATGAAAGA CATGTATGAC 
CAGGTGCTGA AGTTTGGAGC GTATATAGTG GACGGTCTGA GAGAGTACAG ACAGCCGGTT 
CTGGTGTATA TCCCACCACA GGCGGAGCTC CGGGGAGGAT CATGGGTTGT CATAGACCCT 
ACTATCAATC CTCGCCACAT GGAAATGTAT GCAGACAAAG ACAGCCGTGG CGGTGTGCTG 
GAGCCTGAAG GCACAGTGGA GATCAAGTTC CGCAAAAAAG ACCTGGTGAA GACCATGCGA 
CGGGTGGACC CTGTGTACAT GGGGCTTGCA GAAAGACTGG GAACCCCAGA GTTGAGTGTG 
TCTGAGCGTA AAGAGCTGGA GAGTAAACTA AAAGAGAGAG AAGAGTTCCT GCTGCCCATA 
TATCATCAAG TAGCTGTTCA GTTCGCTGAC CTTCATGACA CACCGGGCCG CATGCAGGAG 
AAAGGAGTCA TCACTGATAT CCTTGAGTGG AGCACGTCTC GGCAGTTTTT CTACTGGCGT 
TTGCGGAGGT TATTGCTGGA GGAAACGGTG AAGAGGAAGA TCCAGTGTGC TAACAGTGAG 
CTGACGGACG GACAGGTTCA GGCTATGCTT CGCCGTTGGT TCGTGGAAGC AGAGGGAGCT 
GTGAAAGCGT ATTTGTGGGA CAGTAATGAA GATGTGGTGG AGTGGTTGGA GAGGCAGCTG 
GCAGAGGAAG AAGGCGCCAG ATCCGTCATC GACGAGAATA TCAAATATAT TCGCAGAGAT 
CACATCCTCA AACAGATCCG CAGCCTGGTT CAAGCTAACC CTGAGGTGGC TATGGACTCC 
ATTGTACACA TGACCCAGCA CATTTCCCCC ACACAGCGCG CCGAAGTCGT CCGCATCCTC 
TCCACCATGG ACACCCCAGC CTCCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_017356117.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211606.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X9, mRNA.

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CloneID	ODa59098
Clone ID Related Accession (Same CDS sequence)	XM_017356117.2 , XM_017356117.1
Accession Version	XM_017356117.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGAATG 
TCCTTTTCCT CAAACCTGAA CCACTACGGC ATGGTCCACG TGGCCAGCGT TAGTGATGTG 
CTCCTAGACA CCTCCTTTAC CCCACCCTGT CAGCGCATGG GAGCCATGGT TTCCTTCCGC 
TCTTTCCAGG AGTTCACCAG GAACATAAAG GATGTTCTGA GCTGTTTCTC AGACTCTCCT 
CCATCAACTC CAACCTTTCC TGAAGGGGGG AACCCTGTGC TGTATAGAGA GGAGGACAGC 
AAGAGCATTC AAGATGAGCC CATTCATATT CTTAATGTGG CAATAAAGAC GGACAGTGAT 
ATTGATGATG ATGGGCTGGC GGCCATGTTT CGTGAGTTTA CACAGTCTAA GAAATCTCTG 
CTGTTTGACC ACGGCATCCG AAGACTTACC TTCCTGGTTG CTCAGAAGGA TTTCAGAAAG 
CAAATCAACT GTGAGGTGGA CCAGAGGTTC CATAGAGAAT TCCCCAAATT TTTTACATTC 
CGGGCTCGAG ACAAGTTTGA GGAAGACCGT ATCTACAGAC ACTTGGAACC TGCATTAGCT 
TTCCAGCTGG AGCTCAACCG CATGCGTAAT TTTGCACTGA CAGCCATCCC CTGTGCCAAT 
CATAAGATGC ACCTGTACTT GGGAGCTGCC CGTGTGGAAG TAGGCACTGA GGTCACAGAT 
TACCGCTTCT TTGTGCGGGC AATCATCCGT CACTCAGATT TGGTCACCAA GGAAGCATCA 
TTTGAGTACC TGCACAACGA GGCAGAACGC TTGCTGCTGG AGGCCATGGA TGAACTGGAA 
GTGGCTTTCA ACAACACCAC TGTGCGAACC GACTGCAATC ACATCTTCCT CAACTTCGTT 
CCAACAGTCA TCATGGACCC TTCAAAGATT GAGGAATCTG TGCGCTCCAT GGTCATGCGC 
TACGGCAGTC GCTTGTGGAA GCTCCGTGTG CTTCAAGCCG AGCTGAAGAT CAACATCCGA 
TTGACTCCCA CCGGCAAACA GATCCCCATC CGCCTCTTCC TGACCAACGA GAGTGGCTAC 
TACCTGGACA TCAGCCTGTA CAAGGAAGTC ACTGACTCCC GTACAGGACA GATCATGTTC 
CAGGCATATG GAGATAAGCA GGGTCCTCTT CATGGCATGC TCATCAACAC ACCCTACGTC 
ACAAAAGACC TGCTGCAGTC CAAACGCTTC CAGGCACAGA GCCTCGGCAC TACCTATGTT 
TACGACTTCC CAGAAATGTT TCGCCAGGCT TTAAGGAAGC TGTGGCAATC CATGGATGCA 
CACGCCAACT TGCCCAAGTG CCCTCTTCCA TCAGAACTGC TCACCTTCAC AGAGCTGGTT 
CTGGATTCTC AGGGTCAGCT GGTGCAGATG AACCGCCTAC CTGGAGGGAA TGAGATTGGC 
ATGGTAGCAT GGCGAATGAC CCTCCGGACC CCTGAGTATC CAGCCGGCCG TGAGATTATA 
GTGATCAGCA ATGACATCAC ACATAAGATT GGCTCGTTTG GGCCACAAGA AGATGTTCTG 
TTCCAGCAAG CTTCAGAAAT GGCAAGAGAA AGTGGCATTC CACGGATCTA TATTGCTGCC 
AACAGCGGAG CTCGTATAGG CCTGGCAGAG GAGATCAGAC ACATGTTTCA TGTGGCCTGG 
CAGGATCCGG TGGACCCATA CAAGGGTTTT AAGTACCTAT ACCTCACACC ACAGGACTAT 
AAGAAGGTGT CTGCTCTGAA CTCAGTCCAC TGTGAACATG TGGAGGATGA GGGGGAATCC 
AGGTATAAAA TCACTGATAT CATTGGGAAA GAGGAAGGGT TGGGTGTGGA GAACTTGAGA 
GGTTCTGGCA TGATCGCAGG TGAATCGTCA CTGGCGTATG AGGATATTAT CACCATGAAT 
CTGGTGACAT GCAGGGCCAT TGGAATCGGA GCGTACTTGG TGAGACTGGG CCAAAGAACC 
ATACAGGTGG AAAACTCTCA TATCATCCTG ACTGGAGCCG GAGCTCTCAA TAAGGTTCTC 
GGGCGTGAGG TGTACACTTC CAATAACCAA CTGGGTGGAG TACAGATCAT GCACAATAAC 
GGAGTCACAC ACACCAATGT GTGCGATGAT TTTGAGGGAG TGTATACATT GCTGCACTGG 
CTGTCTTACA TGCCAAAGAA TATGTCCAGC CCTGTGCCGA TACTCTCTGC TAAAGATCCC 
ATTGATCGAG CGATAGAGTT TGTCCCAACC AAGGCACCTT ATGACCCACG CTGGATGCTA 
GCTGGACGTC CCAATCAGAA CATTAAGGGT GCATGGGTGA GTGGGTTCTT CGACCAGGGC 
TCTTTTCTGG AGATCATGCA GCCATGGGCA CAGAGTGTTG TCGTAGGACG GGCCAGGTTG 
GGTGGGATCC CAACTGGAGT AGTTGCCGTG GAAACCCGAT CAGTGGAGCT TTCAATCCCA 
GCAGACCCAG CTAATCTGGA CTCGGAAGCC AAGATAATCC AGCAGGCTGG GCAAGTGTGG 
TTCCCTGATT CTGCTTTCAA AACTGCACAA GCCATTAAAG ACTTCAATAG AGAGGGACTT 
CCGCTTATAG TGTTTGCCAA CTGGAGAGGT TTTTCTGGAG GCATGAAAGA CATGTATGAC 
CAGGTGCTGA AGTTTGGAGC GTATATAGTG GACGGTCTGA GAGAGTACAG ACAGCCGGTT 
CTGGTGTATA TCCCACCACA GGCGGAGCTC CGGGGAGGAT CATGGGTTGT CATAGACCCT 
ACTATCAATC CTCGCCACAT GGAAATGTAT GCAGACAAAG ACAGCCGTGG CGGTGTGCTG 
GAGCCTGAAG GCACAGTGGA GATCAAGTTC CGCAAAAAAG ACCTGGTGAA GACCATGCGA 
CGGGTGGACC CTGTGTACAT GGGGCTTGCA GAAAGACTGG GAACCCCAGA GTTGAGTGTG 
TCTGAGCGTA AAGAGCTGGA GAGTAAACTA AAAGAGAGAG AAGAGTTCCT GCTGCCCATA 
TATCATCAAG TAGCTGTTCA GTTCGCTGAC CTTCATGACA CACCGGGCCG CATGCAGGAG 
AAAGGAGTCA TCACTGATAT CCTTGAGTGG AGCACGTCTC GGCAGTTTTT CTACTGGCGT 
TTGCGGAGGT TATTGCTGGA GGAAACGGTG AAGAGGAAGA TCCAGTGTGC TAACAGTGAG 
CTGACGGACG GACAGGTTCA GGCTATGCTT CGCCGTTGGT TCGTGGAAGC AGAGGGAGCT 
GTGAAAGCGT ATTTGTGGGA CAGTAATGAA GATGTGGTGG AGTGGTTGGA GAGGCAGCTG 
GCAGAGGAAG AAGGCGCCAG ATCCGTCATC GACGAGAATA TCAAATATAT TCGCAGAGAT 
CACATCCTCA AACAGATCCG CAGCCTGGTT CAAGCTAACC CTGAGGTGGC TATGGACTCC 
ATTGTACACA TGACCCAGCA CATTTCCCCC ACACAGCGCG CCGAAGTCGT CCGCATCCTC 
TCCACCATGG ACACCCCAGC CTCCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211606.1
CDS	242..7291
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_017356117.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356117.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGAATG 
TCCTTTTCCT CAAACCTGAA CCACTACGGC ATGGTCCACG TGGCCAGCGT TAGTGATGTG 
CTCCTAGACA CCTCCTTTAC CCCACCCTGT CAGCGCATGG GAGCCATGGT TTCCTTCCGC 
TCTTTCCAGG AGTTCACCAG GAACATAAAG GATGTTCTGA GCTGTTTCTC AGACTCTCCT 
CCATCAACTC CAACCTTTCC TGAAGGGGGG AACCCTGTGC TGTATAGAGA GGAGGACAGC 
AAGAGCATTC AAGATGAGCC CATTCATATT CTTAATGTGG CAATAAAGAC GGACAGTGAT 
ATTGATGATG ATGGGCTGGC GGCCATGTTT CGTGAGTTTA CACAGTCTAA GAAATCTCTG 
CTGTTTGACC ACGGCATCCG AAGACTTACC TTCCTGGTTG CTCAGAAGGA TTTCAGAAAG 
CAAATCAACT GTGAGGTGGA CCAGAGGTTC CATAGAGAAT TCCCCAAATT TTTTACATTC 
CGGGCTCGAG ACAAGTTTGA GGAAGACCGT ATCTACAGAC ACTTGGAACC TGCATTAGCT 
TTCCAGCTGG AGCTCAACCG CATGCGTAAT TTTGCACTGA CAGCCATCCC CTGTGCCAAT 
CATAAGATGC ACCTGTACTT GGGAGCTGCC CGTGTGGAAG TAGGCACTGA GGTCACAGAT 
TACCGCTTCT TTGTGCGGGC AATCATCCGT CACTCAGATT TGGTCACCAA GGAAGCATCA 
TTTGAGTACC TGCACAACGA GGCAGAACGC TTGCTGCTGG AGGCCATGGA TGAACTGGAA 
GTGGCTTTCA ACAACACCAC TGTGCGAACC GACTGCAATC ACATCTTCCT CAACTTCGTT 
CCAACAGTCA TCATGGACCC TTCAAAGATT GAGGAATCTG TGCGCTCCAT GGTCATGCGC 
TACGGCAGTC GCTTGTGGAA GCTCCGTGTG CTTCAAGCCG AGCTGAAGAT CAACATCCGA 
TTGACTCCCA CCGGCAAACA GATCCCCATC CGCCTCTTCC TGACCAACGA GAGTGGCTAC 
TACCTGGACA TCAGCCTGTA CAAGGAAGTC ACTGACTCCC GTACAGGACA GATCATGTTC 
CAGGCATATG GAGATAAGCA GGGTCCTCTT CATGGCATGC TCATCAACAC ACCCTACGTC 
ACAAAAGACC TGCTGCAGTC CAAACGCTTC CAGGCACAGA GCCTCGGCAC TACCTATGTT 
TACGACTTCC CAGAAATGTT TCGCCAGGCT TTAAGGAAGC TGTGGCAATC CATGGATGCA 
CACGCCAACT TGCCCAAGTG CCCTCTTCCA TCAGAACTGC TCACCTTCAC AGAGCTGGTT 
CTGGATTCTC AGGGTCAGCT GGTGCAGATG AACCGCCTAC CTGGAGGGAA TGAGATTGGC 
ATGGTAGCAT GGCGAATGAC CCTCCGGACC CCTGAGTATC CAGCCGGCCG TGAGATTATA 
GTGATCAGCA ATGACATCAC ACATAAGATT GGCTCGTTTG GGCCACAAGA AGATGTTCTG 
TTCCAGCAAG CTTCAGAAAT GGCAAGAGAA AGTGGCATTC CACGGATCTA TATTGCTGCC 
AACAGCGGAG CTCGTATAGG CCTGGCAGAG GAGATCAGAC ACATGTTTCA TGTGGCCTGG 
CAGGATCCGG TGGACCCATA CAAGGGTTTT AAGTACCTAT ACCTCACACC ACAGGACTAT 
AAGAAGGTGT CTGCTCTGAA CTCAGTCCAC TGTGAACATG TGGAGGATGA GGGGGAATCC 
AGGTATAAAA TCACTGATAT CATTGGGAAA GAGGAAGGGT TGGGTGTGGA GAACTTGAGA 
GGTTCTGGCA TGATCGCAGG TGAATCGTCA CTGGCGTATG AGGATATTAT CACCATGAAT 
CTGGTGACAT GCAGGGCCAT TGGAATCGGA GCGTACTTGG TGAGACTGGG CCAAAGAACC 
ATACAGGTGG AAAACTCTCA TATCATCCTG ACTGGAGCCG GAGCTCTCAA TAAGGTTCTC 
GGGCGTGAGG TGTACACTTC CAATAACCAA CTGGGTGGAG TACAGATCAT GCACAATAAC 
GGAGTCACAC ACACCAATGT GTGCGATGAT TTTGAGGGAG TGTATACATT GCTGCACTGG 
CTGTCTTACA TGCCAAAGAA TATGTCCAGC CCTGTGCCGA TACTCTCTGC TAAAGATCCC 
ATTGATCGAG CGATAGAGTT TGTCCCAACC AAGGCACCTT ATGACCCACG CTGGATGCTA 
GCTGGACGTC CCAATCAGAA CATTAAGGGT GCATGGGTGA GTGGGTTCTT CGACCAGGGC 
TCTTTTCTGG AGATCATGCA GCCATGGGCA CAGAGTGTTG TCGTAGGACG GGCCAGGTTG 
GGTGGGATCC CAACTGGAGT AGTTGCCGTG GAAACCCGAT CAGTGGAGCT TTCAATCCCA 
GCAGACCCAG CTAATCTGGA CTCGGAAGCC AAGATAATCC AGCAGGCTGG GCAAGTGTGG 
TTCCCTGATT CTGCTTTCAA AACTGCACAA GCCATTAAAG ACTTCAATAG AGAGGGACTT 
CCGCTTATAG TGTTTGCCAA CTGGAGAGGT TTTTCTGGAG GCATGAAAGA CATGTATGAC 
CAGGTGCTGA AGTTTGGAGC GTATATAGTG GACGGTCTGA GAGAGTACAG ACAGCCGGTT 
CTGGTGTATA TCCCACCACA GGCGGAGCTC CGGGGAGGAT CATGGGTTGT CATAGACCCT 
ACTATCAATC CTCGCCACAT GGAAATGTAT GCAGACAAAG ACAGCCGTGG CGGTGTGCTG 
GAGCCTGAAG GCACAGTGGA GATCAAGTTC CGCAAAAAAG ACCTGGTGAA GACCATGCGA 
CGGGTGGACC CTGTGTACAT GGGGCTTGCA GAAAGACTGG GAACCCCAGA GTTGAGTGTG 
TCTGAGCGTA AAGAGCTGGA GAGTAAACTA AAAGAGAGAG AAGAGTTCCT GCTGCCCATA 
TATCATCAAG TAGCTGTTCA GTTCGCTGAC CTTCATGACA CACCGGGCCG CATGCAGGAG 
AAAGGAGTCA TCACTGATAT CCTTGAGTGG AGCACGTCTC GGCAGTTTTT CTACTGGCGT 
TTGCGGAGGT TATTGCTGGA GGAAACGGTG AAGAGGAAGA TCCAGTGTGC TAACAGTGAG 
CTGACGGACG GACAGGTTCA GGCTATGCTT CGCCGTTGGT TCGTGGAAGC AGAGGGAGCT 
GTGAAAGCGT ATTTGTGGGA CAGTAATGAA GATGTGGTGG AGTGGTTGGA GAGGCAGCTG 
GCAGAGGAAG AAGGCGCCAG ATCCGTCATC GACGAGAATA TCAAATATAT TCGCAGAGAT 
CACATCCTCA AACAGATCCG CAGCCTGGTT CAAGCTAACC CTGAGGTGGC TATGGACTCC 
ATTGTACACA TGACCCAGCA CATTTCCCCC ACACAGCGCG CCGAAGTCGT CCGCATCCTC 
TCCACCATGG ACACCCCAGC CTCCTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( NM_001271308.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001258237.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), mRNA.

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CloneID	ODa03022
Clone ID Related Accession (Same CDS sequence)	XM_017356109.1 , XM_017356110.1 , XM_017356109.2 , NM_001271308.1
Accession Version	NM_001271308.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL935115.6 and BX255908.4. On Sep 13, 2012 this sequence version replaced XM_003198687.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGA TTGAGGAATC TGTGCGCTCC ATGGTCATGC GCTACGGCAG TCGCTTGTGG 
AAGCTCCGTG TGCTTCAAGC CGAGCTGAAG ATCAACATCC GATTGACTCC CACCGGCAAA 
CAGATCCCCA TCCGCCTCTT CCTGACCAAC GAGAGTGGCT ACTACCTGGA CATCAGCCTG 
TACAAGGAAG TCACTGACTC CCGTACAGGA CAGGTGGGGC ACAAAGACCG ACAGATCATG 
TTCCAGGCAT ATGGAGATAA GCAGGGTCCT CTTCATGGCA TGCTCATCAA CACACCCTAC 
GTCACAAAAG ACCTGCTGCA GTCCAAACGC TTCCAGGCAC AGAGCCTCGG CACTACCTAT 
GTTTACGACT TCCCAGAAAT GTTTCGCCAG GCTTTAAGGA AGCTGTGGCA ATCCATGGAT 
GCACACGCCA ACTTGCCCAA GTGCCCTCTT CCATCAGAAC TGCTCACCTT CACAGAGCTG 
GTTCTGGATT CTCAGGGTCA GCTGGTGCAG ATGAACCGCC TACCTGGAGG GAATGAGATT 
GGCATGGTAG CATGGCGAAT GACCCTCCGG ACCCCTGAGT ATCCAGCCGG CCGTGAGATT 
ATAGTGATCA GCAATGACAT CACACATAAG ATTGGCTCGT TTGGGCCACA AGAAGATGTT 
CTGTTCCAGC AAGCTTCAGA AATGGCAAGA GAAAGTGGCA TTCCACGGAT CTATATTGCT 
GCCAACAGCG GAGCTCGTAT AGGCCTGGCA GAGGAGATCA GACACATGTT TCATGTGGCC 
TGGCAGGATC CGGTGGACCC ATACAAGGGT TTTAAGTACC TATACCTCAC ACCACAGGAC 
TATAAGAAGG TGTCTGCTCT GAACTCAGTC CACTGTGAAC ATGTGGAGGA TGAGGGGGAA 
TCCAGGTATA AAATCACTGA TATCATTGGG AAAGAGGAAG GGTTGGGTGT GGAGAACTTG 
AGAGGTTCTG GCATGATCGC AGGTGAATCG TCACTGGCGT ATGAGGATAT TATCACCATG 
AATCTGGTGA CATGCAGGGC CATTGGAATC GGAGCGTACT TGGTGAGACT GGGCCAAAGA 
ACCATACAGG TGGAAAACTC TCATATCATC CTGACTGGAG CCGGAGCTCT CAATAAGGTT 
CTCGGGCGTG AGGTGTACAC TTCCAATAAC CAACTGGGTG GAGTACAGAT CATGCACAAT 
AACGGAGTCA CACACACCAA TGTGTGCGAT GATTTTGAGG GAGTGTATAC ATTGCTGCAC 
TGGCTGTCTT ACATGCCAAA GAATATGTCC AGCCCTGTGC CGATACTCTC TGCTAAAGAT 
CCCATTGATC GAGCGATAGA GTTTGTCCCA ACCAAGGCAC CTTATGACCC ACGCTGGATG 
CTAGCTGGAC GTCCCAATCA GAACATTAAG GGTGCATGGG TGAGTGGGTT CTTCGACCAG 
GGCTCTTTTC TGGAGATCAT GCAGCCATGG GCACAGAGTG TTGTCGTAGG ACGGGCCAGG 
TTGGGTGGGA TCCCAACTGG AGTAGTTGCC GTGGAAACCC GATCAGTGGA GCTTTCAATC 
CCAGCAGACC CAGCTAATCT GGACTCGGAA GCCAAGATAA TCCAGCAGGC TGGGCAAGTG 
TGGTTCCCTG ATTCTGCTTT CAAAACTGCA CAAGCCATTA AAGACTTCAA TAGAGAGGGA 
CTTCCGCTTA TAGTGTTTGC CAACTGGAGA GGTTTTTCTG GAGGCATGAA AGACATGTAT 
GACCAGGTGC TGAAGTTTGG AGCGTATATA GTGGACGGTC TGAGAGAGTA CAGACAGCCG 
GTTCTGGTGT ATATCCCACC ACAGGCGGAG CTCCGGGGAG GATCATGGGT TGTCATAGAC 
CCTACTATCA ATCCTCGCCA CATGGAAATG TATGCAGACA AAGACAGCCG TGGCGGTGTG 
CTGGAGCCTG AAGGCACAGT GGAGATCAAG TTCCGCAAAA AAGACCTGGT GAAGACCATG 
CGACGGGTGG ACCCTGTGTA CATGGGGCTT GCAGAAAGAC TGGGAACCCC AGAGTTGAGT 
GTGTCTGAGC GTAAAGAGCT GGAGAGTAAA CTAAAAGAGA GAGAAGAGTT CCTGCTGCCC 
ATATATCATC AAGTAGCTGT TCAGTTCGCT GACCTTCATG ACACACCGGG CCGCATGCAG 
GAGAAAGGAG TCATCACTGA TATCCTTGAG TGGAGCACGT CTCGGCAGTT TTTCTACTGG 
CGTTTGCGGA GGTTATTGCT GGAGGAAACG GTGAAGAGGA AGATCCAGTG TGCTAACAGT 
GAGCTGACGG ACGGACAGGT TCAGGCTATG CTTCGCCGTT GGTTCGTGGA AGCAGAGGGA 
GCTGTGAAAG CGTATTTGTG GGACAGTAAT GAAGATGTGG TGGAGTGGTT GGAGAGGCAG 
CTGGCAGAGG AAGAAGGCGC CAGATCCGTC ATCGACGAGA ATATCAAATA TATTCGCAGA 
GATCACATCC TCAAACAGAT CCGCAGCCTG GTTCAAGCTA ACCCTGAGGT GGCTATGGAC 
TCCATTGTAC ACATGACCCA GCACATTTCC CCCACACAGC GCGCCGAAGT CGTCCGCATC 
CTCTCCACCA TGGACACCCC AGCCTCCTCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258237.1
CDS	1..7173
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRKLSAPLQDYKPPANTANTSGATEAASDSAESEDRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	NM_001271308.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271308.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGA TTGAGGAATC TGTGCGCTCC ATGGTCATGC GCTACGGCAG TCGCTTGTGG 
AAGCTCCGTG TGCTTCAAGC CGAGCTGAAG ATCAACATCC GATTGACTCC CACCGGCAAA 
CAGATCCCCA TCCGCCTCTT CCTGACCAAC GAGAGTGGCT ACTACCTGGA CATCAGCCTG 
TACAAGGAAG TCACTGACTC CCGTACAGGA CAGGTGGGGC ACAAAGACCG ACAGATCATG 
TTCCAGGCAT ATGGAGATAA GCAGGGTCCT CTTCATGGCA TGCTCATCAA CACACCCTAC 
GTCACAAAAG ACCTGCTGCA GTCCAAACGC TTCCAGGCAC AGAGCCTCGG CACTACCTAT 
GTTTACGACT TCCCAGAAAT GTTTCGCCAG GCTTTAAGGA AGCTGTGGCA ATCCATGGAT 
GCACACGCCA ACTTGCCCAA GTGCCCTCTT CCATCAGAAC TGCTCACCTT CACAGAGCTG 
GTTCTGGATT CTCAGGGTCA GCTGGTGCAG ATGAACCGCC TACCTGGAGG GAATGAGATT 
GGCATGGTAG CATGGCGAAT GACCCTCCGG ACCCCTGAGT ATCCAGCCGG CCGTGAGATT 
ATAGTGATCA GCAATGACAT CACACATAAG ATTGGCTCGT TTGGGCCACA AGAAGATGTT 
CTGTTCCAGC AAGCTTCAGA AATGGCAAGA GAAAGTGGCA TTCCACGGAT CTATATTGCT 
GCCAACAGCG GAGCTCGTAT AGGCCTGGCA GAGGAGATCA GACACATGTT TCATGTGGCC 
TGGCAGGATC CGGTGGACCC ATACAAGGGT TTTAAGTACC TATACCTCAC ACCACAGGAC 
TATAAGAAGG TGTCTGCTCT GAACTCAGTC CACTGTGAAC ATGTGGAGGA TGAGGGGGAA 
TCCAGGTATA AAATCACTGA TATCATTGGG AAAGAGGAAG GGTTGGGTGT GGAGAACTTG 
AGAGGTTCTG GCATGATCGC AGGTGAATCG TCACTGGCGT ATGAGGATAT TATCACCATG 
AATCTGGTGA CATGCAGGGC CATTGGAATC GGAGCGTACT TGGTGAGACT GGGCCAAAGA 
ACCATACAGG TGGAAAACTC TCATATCATC CTGACTGGAG CCGGAGCTCT CAATAAGGTT 
CTCGGGCGTG AGGTGTACAC TTCCAATAAC CAACTGGGTG GAGTACAGAT CATGCACAAT 
AACGGAGTCA CACACACCAA TGTGTGCGAT GATTTTGAGG GAGTGTATAC ATTGCTGCAC 
TGGCTGTCTT ACATGCCAAA GAATATGTCC AGCCCTGTGC CGATACTCTC TGCTAAAGAT 
CCCATTGATC GAGCGATAGA GTTTGTCCCA ACCAAGGCAC CTTATGACCC ACGCTGGATG 
CTAGCTGGAC GTCCCAATCA GAACATTAAG GGTGCATGGG TGAGTGGGTT CTTCGACCAG 
GGCTCTTTTC TGGAGATCAT GCAGCCATGG GCACAGAGTG TTGTCGTAGG ACGGGCCAGG 
TTGGGTGGGA TCCCAACTGG AGTAGTTGCC GTGGAAACCC GATCAGTGGA GCTTTCAATC 
CCAGCAGACC CAGCTAATCT GGACTCGGAA GCCAAGATAA TCCAGCAGGC TGGGCAAGTG 
TGGTTCCCTG ATTCTGCTTT CAAAACTGCA CAAGCCATTA AAGACTTCAA TAGAGAGGGA 
CTTCCGCTTA TAGTGTTTGC CAACTGGAGA GGTTTTTCTG GAGGCATGAA AGACATGTAT 
GACCAGGTGC TGAAGTTTGG AGCGTATATA GTGGACGGTC TGAGAGAGTA CAGACAGCCG 
GTTCTGGTGT ATATCCCACC ACAGGCGGAG CTCCGGGGAG GATCATGGGT TGTCATAGAC 
CCTACTATCA ATCCTCGCCA CATGGAAATG TATGCAGACA AAGACAGCCG TGGCGGTGTG 
CTGGAGCCTG AAGGCACAGT GGAGATCAAG TTCCGCAAAA AAGACCTGGT GAAGACCATG 
CGACGGGTGG ACCCTGTGTA CATGGGGCTT GCAGAAAGAC TGGGAACCCC AGAGTTGAGT 
GTGTCTGAGC GTAAAGAGCT GGAGAGTAAA CTAAAAGAGA GAGAAGAGTT CCTGCTGCCC 
ATATATCATC AAGTAGCTGT TCAGTTCGCT GACCTTCATG ACACACCGGG CCGCATGCAG 
GAGAAAGGAG TCATCACTGA TATCCTTGAG TGGAGCACGT CTCGGCAGTT TTTCTACTGG 
CGTTTGCGGA GGTTATTGCT GGAGGAAACG GTGAAGAGGA AGATCCAGTG TGCTAACAGT 
GAGCTGACGG ACGGACAGGT TCAGGCTATG CTTCGCCGTT GGTTCGTGGA AGCAGAGGGA 
GCTGTGAAAG CGTATTTGTG GGACAGTAAT GAAGATGTGG TGGAGTGGTT GGAGAGGCAG 
CTGGCAGAGG AAGAAGGCGC CAGATCCGTC ATCGACGAGA ATATCAAATA TATTCGCAGA 
GATCACATCC TCAAACAGAT CCGCAGCCTG GTTCAAGCTA ACCCTGAGGT GGCTATGGAC 
TCCATTGTAC ACATGACCCA GCACATTTCC CCCACACAGC GCGCCGAAGT CGTCCGCATC 
CTCTCCACCA TGGACACCCC AGCCTCCTCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_017356109.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211598.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X1, mRNA.

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CloneID	ODa03022
Clone ID Related Accession (Same CDS sequence)	XM_017356109.1 , XM_017356110.1 , XM_017356109.2 , NM_001271308.1
Accession Version	XM_017356109.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGA TTGAGGAATC TGTGCGCTCC ATGGTCATGC GCTACGGCAG TCGCTTGTGG 
AAGCTCCGTG TGCTTCAAGC CGAGCTGAAG ATCAACATCC GATTGACTCC CACCGGCAAA 
CAGATCCCCA TCCGCCTCTT CCTGACCAAC GAGAGTGGCT ACTACCTGGA CATCAGCCTG 
TACAAGGAAG TCACTGACTC CCGTACAGGA CAGGTGGGGC ACAAAGACCG ACAGATCATG 
TTCCAGGCAT ATGGAGATAA GCAGGGTCCT CTTCATGGCA TGCTCATCAA CACACCCTAC 
GTCACAAAAG ACCTGCTGCA GTCCAAACGC TTCCAGGCAC AGAGCCTCGG CACTACCTAT 
GTTTACGACT TCCCAGAAAT GTTTCGCCAG GCTTTAAGGA AGCTGTGGCA ATCCATGGAT 
GCACACGCCA ACTTGCCCAA GTGCCCTCTT CCATCAGAAC TGCTCACCTT CACAGAGCTG 
GTTCTGGATT CTCAGGGTCA GCTGGTGCAG ATGAACCGCC TACCTGGAGG GAATGAGATT 
GGCATGGTAG CATGGCGAAT GACCCTCCGG ACCCCTGAGT ATCCAGCCGG CCGTGAGATT 
ATAGTGATCA GCAATGACAT CACACATAAG ATTGGCTCGT TTGGGCCACA AGAAGATGTT 
CTGTTCCAGC AAGCTTCAGA AATGGCAAGA GAAAGTGGCA TTCCACGGAT CTATATTGCT 
GCCAACAGCG GAGCTCGTAT AGGCCTGGCA GAGGAGATCA GACACATGTT TCATGTGGCC 
TGGCAGGATC CGGTGGACCC ATACAAGGGT TTTAAGTACC TATACCTCAC ACCACAGGAC 
TATAAGAAGG TGTCTGCTCT GAACTCAGTC CACTGTGAAC ATGTGGAGGA TGAGGGGGAA 
TCCAGGTATA AAATCACTGA TATCATTGGG AAAGAGGAAG GGTTGGGTGT GGAGAACTTG 
AGAGGTTCTG GCATGATCGC AGGTGAATCG TCACTGGCGT ATGAGGATAT TATCACCATG 
AATCTGGTGA CATGCAGGGC CATTGGAATC GGAGCGTACT TGGTGAGACT GGGCCAAAGA 
ACCATACAGG TGGAAAACTC TCATATCATC CTGACTGGAG CCGGAGCTCT CAATAAGGTT 
CTCGGGCGTG AGGTGTACAC TTCCAATAAC CAACTGGGTG GAGTACAGAT CATGCACAAT 
AACGGAGTCA CACACACCAA TGTGTGCGAT GATTTTGAGG GAGTGTATAC ATTGCTGCAC 
TGGCTGTCTT ACATGCCAAA GAATATGTCC AGCCCTGTGC CGATACTCTC TGCTAAAGAT 
CCCATTGATC GAGCGATAGA GTTTGTCCCA ACCAAGGCAC CTTATGACCC ACGCTGGATG 
CTAGCTGGAC GTCCCAATCA GAACATTAAG GGTGCATGGG TGAGTGGGTT CTTCGACCAG 
GGCTCTTTTC TGGAGATCAT GCAGCCATGG GCACAGAGTG TTGTCGTAGG ACGGGCCAGG 
TTGGGTGGGA TCCCAACTGG AGTAGTTGCC GTGGAAACCC GATCAGTGGA GCTTTCAATC 
CCAGCAGACC CAGCTAATCT GGACTCGGAA GCCAAGATAA TCCAGCAGGC TGGGCAAGTG 
TGGTTCCCTG ATTCTGCTTT CAAAACTGCA CAAGCCATTA AAGACTTCAA TAGAGAGGGA 
CTTCCGCTTA TAGTGTTTGC CAACTGGAGA GGTTTTTCTG GAGGCATGAA AGACATGTAT 
GACCAGGTGC TGAAGTTTGG AGCGTATATA GTGGACGGTC TGAGAGAGTA CAGACAGCCG 
GTTCTGGTGT ATATCCCACC ACAGGCGGAG CTCCGGGGAG GATCATGGGT TGTCATAGAC 
CCTACTATCA ATCCTCGCCA CATGGAAATG TATGCAGACA AAGACAGCCG TGGCGGTGTG 
CTGGAGCCTG AAGGCACAGT GGAGATCAAG TTCCGCAAAA AAGACCTGGT GAAGACCATG 
CGACGGGTGG ACCCTGTGTA CATGGGGCTT GCAGAAAGAC TGGGAACCCC AGAGTTGAGT 
GTGTCTGAGC GTAAAGAGCT GGAGAGTAAA CTAAAAGAGA GAGAAGAGTT CCTGCTGCCC 
ATATATCATC AAGTAGCTGT TCAGTTCGCT GACCTTCATG ACACACCGGG CCGCATGCAG 
GAGAAAGGAG TCATCACTGA TATCCTTGAG TGGAGCACGT CTCGGCAGTT TTTCTACTGG 
CGTTTGCGGA GGTTATTGCT GGAGGAAACG GTGAAGAGGA AGATCCAGTG TGCTAACAGT 
GAGCTGACGG ACGGACAGGT TCAGGCTATG CTTCGCCGTT GGTTCGTGGA AGCAGAGGGA 
GCTGTGAAAG CGTATTTGTG GGACAGTAAT GAAGATGTGG TGGAGTGGTT GGAGAGGCAG 
CTGGCAGAGG AAGAAGGCGC CAGATCCGTC ATCGACGAGA ATATCAAATA TATTCGCAGA 
GATCACATCC TCAAACAGAT CCGCAGCCTG GTTCAAGCTA ACCCTGAGGT GGCTATGGAC 
TCCATTGTAC ACATGACCCA GCACATTTCC CCCACACAGC GCGCCGAAGT CGTCCGCATC 
CTCTCCACCA TGGACACCCC AGCCTCCTCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211598.1
CDS	242..7414
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRKLSAPLQDYKPPANTANTSGATEAASDSAESEDRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_017356109.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356109.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGA TTGAGGAATC TGTGCGCTCC ATGGTCATGC GCTACGGCAG TCGCTTGTGG 
AAGCTCCGTG TGCTTCAAGC CGAGCTGAAG ATCAACATCC GATTGACTCC CACCGGCAAA 
CAGATCCCCA TCCGCCTCTT CCTGACCAAC GAGAGTGGCT ACTACCTGGA CATCAGCCTG 
TACAAGGAAG TCACTGACTC CCGTACAGGA CAGGTGGGGC ACAAAGACCG ACAGATCATG 
TTCCAGGCAT ATGGAGATAA GCAGGGTCCT CTTCATGGCA TGCTCATCAA CACACCCTAC 
GTCACAAAAG ACCTGCTGCA GTCCAAACGC TTCCAGGCAC AGAGCCTCGG CACTACCTAT 
GTTTACGACT TCCCAGAAAT GTTTCGCCAG GCTTTAAGGA AGCTGTGGCA ATCCATGGAT 
GCACACGCCA ACTTGCCCAA GTGCCCTCTT CCATCAGAAC TGCTCACCTT CACAGAGCTG 
GTTCTGGATT CTCAGGGTCA GCTGGTGCAG ATGAACCGCC TACCTGGAGG GAATGAGATT 
GGCATGGTAG CATGGCGAAT GACCCTCCGG ACCCCTGAGT ATCCAGCCGG CCGTGAGATT 
ATAGTGATCA GCAATGACAT CACACATAAG ATTGGCTCGT TTGGGCCACA AGAAGATGTT 
CTGTTCCAGC AAGCTTCAGA AATGGCAAGA GAAAGTGGCA TTCCACGGAT CTATATTGCT 
GCCAACAGCG GAGCTCGTAT AGGCCTGGCA GAGGAGATCA GACACATGTT TCATGTGGCC 
TGGCAGGATC CGGTGGACCC ATACAAGGGT TTTAAGTACC TATACCTCAC ACCACAGGAC 
TATAAGAAGG TGTCTGCTCT GAACTCAGTC CACTGTGAAC ATGTGGAGGA TGAGGGGGAA 
TCCAGGTATA AAATCACTGA TATCATTGGG AAAGAGGAAG GGTTGGGTGT GGAGAACTTG 
AGAGGTTCTG GCATGATCGC AGGTGAATCG TCACTGGCGT ATGAGGATAT TATCACCATG 
AATCTGGTGA CATGCAGGGC CATTGGAATC GGAGCGTACT TGGTGAGACT GGGCCAAAGA 
ACCATACAGG TGGAAAACTC TCATATCATC CTGACTGGAG CCGGAGCTCT CAATAAGGTT 
CTCGGGCGTG AGGTGTACAC TTCCAATAAC CAACTGGGTG GAGTACAGAT CATGCACAAT 
AACGGAGTCA CACACACCAA TGTGTGCGAT GATTTTGAGG GAGTGTATAC ATTGCTGCAC 
TGGCTGTCTT ACATGCCAAA GAATATGTCC AGCCCTGTGC CGATACTCTC TGCTAAAGAT 
CCCATTGATC GAGCGATAGA GTTTGTCCCA ACCAAGGCAC CTTATGACCC ACGCTGGATG 
CTAGCTGGAC GTCCCAATCA GAACATTAAG GGTGCATGGG TGAGTGGGTT CTTCGACCAG 
GGCTCTTTTC TGGAGATCAT GCAGCCATGG GCACAGAGTG TTGTCGTAGG ACGGGCCAGG 
TTGGGTGGGA TCCCAACTGG AGTAGTTGCC GTGGAAACCC GATCAGTGGA GCTTTCAATC 
CCAGCAGACC CAGCTAATCT GGACTCGGAA GCCAAGATAA TCCAGCAGGC TGGGCAAGTG 
TGGTTCCCTG ATTCTGCTTT CAAAACTGCA CAAGCCATTA AAGACTTCAA TAGAGAGGGA 
CTTCCGCTTA TAGTGTTTGC CAACTGGAGA GGTTTTTCTG GAGGCATGAA AGACATGTAT 
GACCAGGTGC TGAAGTTTGG AGCGTATATA GTGGACGGTC TGAGAGAGTA CAGACAGCCG 
GTTCTGGTGT ATATCCCACC ACAGGCGGAG CTCCGGGGAG GATCATGGGT TGTCATAGAC 
CCTACTATCA ATCCTCGCCA CATGGAAATG TATGCAGACA AAGACAGCCG TGGCGGTGTG 
CTGGAGCCTG AAGGCACAGT GGAGATCAAG TTCCGCAAAA AAGACCTGGT GAAGACCATG 
CGACGGGTGG ACCCTGTGTA CATGGGGCTT GCAGAAAGAC TGGGAACCCC AGAGTTGAGT 
GTGTCTGAGC GTAAAGAGCT GGAGAGTAAA CTAAAAGAGA GAGAAGAGTT CCTGCTGCCC 
ATATATCATC AAGTAGCTGT TCAGTTCGCT GACCTTCATG ACACACCGGG CCGCATGCAG 
GAGAAAGGAG TCATCACTGA TATCCTTGAG TGGAGCACGT CTCGGCAGTT TTTCTACTGG 
CGTTTGCGGA GGTTATTGCT GGAGGAAACG GTGAAGAGGA AGATCCAGTG TGCTAACAGT 
GAGCTGACGG ACGGACAGGT TCAGGCTATG CTTCGCCGTT GGTTCGTGGA AGCAGAGGGA 
GCTGTGAAAG CGTATTTGTG GGACAGTAAT GAAGATGTGG TGGAGTGGTT GGAGAGGCAG 
CTGGCAGAGG AAGAAGGCGC CAGATCCGTC ATCGACGAGA ATATCAAATA TATTCGCAGA 
GATCACATCC TCAAACAGAT CCGCAGCCTG GTTCAAGCTA ACCCTGAGGT GGCTATGGAC 
TCCATTGTAC ACATGACCCA GCACATTTCC CCCACACAGC GCGCCGAAGT CGTCCGCATC 
CTCTCCACCA TGGACACCCC AGCCTCCTCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_017356110.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211599.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X2, mRNA.

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CloneID	ODa03022
Clone ID Related Accession (Same CDS sequence)	XM_017356109.1 , XM_017356110.1 , XM_017356109.2 , NM_001271308.1
Accession Version	XM_017356110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGA TTGAGGAATC TGTGCGCTCC ATGGTCATGC GCTACGGCAG TCGCTTGTGG 
AAGCTCCGTG TGCTTCAAGC CGAGCTGAAG ATCAACATCC GATTGACTCC CACCGGCAAA 
CAGATCCCCA TCCGCCTCTT CCTGACCAAC GAGAGTGGCT ACTACCTGGA CATCAGCCTG 
TACAAGGAAG TCACTGACTC CCGTACAGGA CAGGTGGGGC ACAAAGACCG ACAGATCATG 
TTCCAGGCAT ATGGAGATAA GCAGGGTCCT CTTCATGGCA TGCTCATCAA CACACCCTAC 
GTCACAAAAG ACCTGCTGCA GTCCAAACGC TTCCAGGCAC AGAGCCTCGG CACTACCTAT 
GTTTACGACT TCCCAGAAAT GTTTCGCCAG GCTTTAAGGA AGCTGTGGCA ATCCATGGAT 
GCACACGCCA ACTTGCCCAA GTGCCCTCTT CCATCAGAAC TGCTCACCTT CACAGAGCTG 
GTTCTGGATT CTCAGGGTCA GCTGGTGCAG ATGAACCGCC TACCTGGAGG GAATGAGATT 
GGCATGGTAG CATGGCGAAT GACCCTCCGG ACCCCTGAGT ATCCAGCCGG CCGTGAGATT 
ATAGTGATCA GCAATGACAT CACACATAAG ATTGGCTCGT TTGGGCCACA AGAAGATGTT 
CTGTTCCAGC AAGCTTCAGA AATGGCAAGA GAAAGTGGCA TTCCACGGAT CTATATTGCT 
GCCAACAGCG GAGCTCGTAT AGGCCTGGCA GAGGAGATCA GACACATGTT TCATGTGGCC 
TGGCAGGATC CGGTGGACCC ATACAAGGGT TTTAAGTACC TATACCTCAC ACCACAGGAC 
TATAAGAAGG TGTCTGCTCT GAACTCAGTC CACTGTGAAC ATGTGGAGGA TGAGGGGGAA 
TCCAGGTATA AAATCACTGA TATCATTGGG AAAGAGGAAG GGTTGGGTGT GGAGAACTTG 
AGAGGTTCTG GCATGATCGC AGGTGAATCG TCACTGGCGT ATGAGGATAT TATCACCATG 
AATCTGGTGA CATGCAGGGC CATTGGAATC GGAGCGTACT TGGTGAGACT GGGCCAAAGA 
ACCATACAGG TGGAAAACTC TCATATCATC CTGACTGGAG CCGGAGCTCT CAATAAGGTT 
CTCGGGCGTG AGGTGTACAC TTCCAATAAC CAACTGGGTG GAGTACAGAT CATGCACAAT 
AACGGAGTCA CACACACCAA TGTGTGCGAT GATTTTGAGG GAGTGTATAC ATTGCTGCAC 
TGGCTGTCTT ACATGCCAAA GAATATGTCC AGCCCTGTGC CGATACTCTC TGCTAAAGAT 
CCCATTGATC GAGCGATAGA GTTTGTCCCA ACCAAGGCAC CTTATGACCC ACGCTGGATG 
CTAGCTGGAC GTCCCAATCA GAACATTAAG GGTGCATGGG TGAGTGGGTT CTTCGACCAG 
GGCTCTTTTC TGGAGATCAT GCAGCCATGG GCACAGAGTG TTGTCGTAGG ACGGGCCAGG 
TTGGGTGGGA TCCCAACTGG AGTAGTTGCC GTGGAAACCC GATCAGTGGA GCTTTCAATC 
CCAGCAGACC CAGCTAATCT GGACTCGGAA GCCAAGATAA TCCAGCAGGC TGGGCAAGTG 
TGGTTCCCTG ATTCTGCTTT CAAAACTGCA CAAGCCATTA AAGACTTCAA TAGAGAGGGA 
CTTCCGCTTA TAGTGTTTGC CAACTGGAGA GGTTTTTCTG GAGGCATGAA AGACATGTAT 
GACCAGGTGC TGAAGTTTGG AGCGTATATA GTGGACGGTC TGAGAGAGTA CAGACAGCCG 
GTTCTGGTGT ATATCCCACC ACAGGCGGAG CTCCGGGGAG GATCATGGGT TGTCATAGAC 
CCTACTATCA ATCCTCGCCA CATGGAAATG TATGCAGACA AAGACAGCCG TGGCGGTGTG 
CTGGAGCCTG AAGGCACAGT GGAGATCAAG TTCCGCAAAA AAGACCTGGT GAAGACCATG 
CGACGGGTGG ACCCTGTGTA CATGGGGCTT GCAGAAAGAC TGGGAACCCC AGAGTTGAGT 
GTGTCTGAGC GTAAAGAGCT GGAGAGTAAA CTAAAAGAGA GAGAAGAGTT CCTGCTGCCC 
ATATATCATC AAGTAGCTGT TCAGTTCGCT GACCTTCATG ACACACCGGG CCGCATGCAG 
GAGAAAGGAG TCATCACTGA TATCCTTGAG TGGAGCACGT CTCGGCAGTT TTTCTACTGG 
CGTTTGCGGA GGTTATTGCT GGAGGAAACG GTGAAGAGGA AGATCCAGTG TGCTAACAGT 
GAGCTGACGG ACGGACAGGT TCAGGCTATG CTTCGCCGTT GGTTCGTGGA AGCAGAGGGA 
GCTGTGAAAG CGTATTTGTG GGACAGTAAT GAAGATGTGG TGGAGTGGTT GGAGAGGCAG 
CTGGCAGAGG AAGAAGGCGC CAGATCCGTC ATCGACGAGA ATATCAAATA TATTCGCAGA 
GATCACATCC TCAAACAGAT CCGCAGCCTG GTTCAAGCTA ACCCTGAGGT GGCTATGGAC 
TCCATTGTAC ACATGACCCA GCACATTTCC CCCACACAGC GCGCCGAAGT CGTCCGCATC 
CTCTCCACCA TGGACACCCC AGCCTCCTCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211599.1
CDS	102..7274
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRKLSAPLQDYKPPANTANTSGATEAASDSAESEDRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_017356110.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356110.1

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGA TTGAGGAATC TGTGCGCTCC ATGGTCATGC GCTACGGCAG TCGCTTGTGG 
AAGCTCCGTG TGCTTCAAGC CGAGCTGAAG ATCAACATCC GATTGACTCC CACCGGCAAA 
CAGATCCCCA TCCGCCTCTT CCTGACCAAC GAGAGTGGCT ACTACCTGGA CATCAGCCTG 
TACAAGGAAG TCACTGACTC CCGTACAGGA CAGGTGGGGC ACAAAGACCG ACAGATCATG 
TTCCAGGCAT ATGGAGATAA GCAGGGTCCT CTTCATGGCA TGCTCATCAA CACACCCTAC 
GTCACAAAAG ACCTGCTGCA GTCCAAACGC TTCCAGGCAC AGAGCCTCGG CACTACCTAT 
GTTTACGACT TCCCAGAAAT GTTTCGCCAG GCTTTAAGGA AGCTGTGGCA ATCCATGGAT 
GCACACGCCA ACTTGCCCAA GTGCCCTCTT CCATCAGAAC TGCTCACCTT CACAGAGCTG 
GTTCTGGATT CTCAGGGTCA GCTGGTGCAG ATGAACCGCC TACCTGGAGG GAATGAGATT 
GGCATGGTAG CATGGCGAAT GACCCTCCGG ACCCCTGAGT ATCCAGCCGG CCGTGAGATT 
ATAGTGATCA GCAATGACAT CACACATAAG ATTGGCTCGT TTGGGCCACA AGAAGATGTT 
CTGTTCCAGC AAGCTTCAGA AATGGCAAGA GAAAGTGGCA TTCCACGGAT CTATATTGCT 
GCCAACAGCG GAGCTCGTAT AGGCCTGGCA GAGGAGATCA GACACATGTT TCATGTGGCC 
TGGCAGGATC CGGTGGACCC ATACAAGGGT TTTAAGTACC TATACCTCAC ACCACAGGAC 
TATAAGAAGG TGTCTGCTCT GAACTCAGTC CACTGTGAAC ATGTGGAGGA TGAGGGGGAA 
TCCAGGTATA AAATCACTGA TATCATTGGG AAAGAGGAAG GGTTGGGTGT GGAGAACTTG 
AGAGGTTCTG GCATGATCGC AGGTGAATCG TCACTGGCGT ATGAGGATAT TATCACCATG 
AATCTGGTGA CATGCAGGGC CATTGGAATC GGAGCGTACT TGGTGAGACT GGGCCAAAGA 
ACCATACAGG TGGAAAACTC TCATATCATC CTGACTGGAG CCGGAGCTCT CAATAAGGTT 
CTCGGGCGTG AGGTGTACAC TTCCAATAAC CAACTGGGTG GAGTACAGAT CATGCACAAT 
AACGGAGTCA CACACACCAA TGTGTGCGAT GATTTTGAGG GAGTGTATAC ATTGCTGCAC 
TGGCTGTCTT ACATGCCAAA GAATATGTCC AGCCCTGTGC CGATACTCTC TGCTAAAGAT 
CCCATTGATC GAGCGATAGA GTTTGTCCCA ACCAAGGCAC CTTATGACCC ACGCTGGATG 
CTAGCTGGAC GTCCCAATCA GAACATTAAG GGTGCATGGG TGAGTGGGTT CTTCGACCAG 
GGCTCTTTTC TGGAGATCAT GCAGCCATGG GCACAGAGTG TTGTCGTAGG ACGGGCCAGG 
TTGGGTGGGA TCCCAACTGG AGTAGTTGCC GTGGAAACCC GATCAGTGGA GCTTTCAATC 
CCAGCAGACC CAGCTAATCT GGACTCGGAA GCCAAGATAA TCCAGCAGGC TGGGCAAGTG 
TGGTTCCCTG ATTCTGCTTT CAAAACTGCA CAAGCCATTA AAGACTTCAA TAGAGAGGGA 
CTTCCGCTTA TAGTGTTTGC CAACTGGAGA GGTTTTTCTG GAGGCATGAA AGACATGTAT 
GACCAGGTGC TGAAGTTTGG AGCGTATATA GTGGACGGTC TGAGAGAGTA CAGACAGCCG 
GTTCTGGTGT ATATCCCACC ACAGGCGGAG CTCCGGGGAG GATCATGGGT TGTCATAGAC 
CCTACTATCA ATCCTCGCCA CATGGAAATG TATGCAGACA AAGACAGCCG TGGCGGTGTG 
CTGGAGCCTG AAGGCACAGT GGAGATCAAG TTCCGCAAAA AAGACCTGGT GAAGACCATG 
CGACGGGTGG ACCCTGTGTA CATGGGGCTT GCAGAAAGAC TGGGAACCCC AGAGTTGAGT 
GTGTCTGAGC GTAAAGAGCT GGAGAGTAAA CTAAAAGAGA GAGAAGAGTT CCTGCTGCCC 
ATATATCATC AAGTAGCTGT TCAGTTCGCT GACCTTCATG ACACACCGGG CCGCATGCAG 
GAGAAAGGAG TCATCACTGA TATCCTTGAG TGGAGCACGT CTCGGCAGTT TTTCTACTGG 
CGTTTGCGGA GGTTATTGCT GGAGGAAACG GTGAAGAGGA AGATCCAGTG TGCTAACAGT 
GAGCTGACGG ACGGACAGGT TCAGGCTATG CTTCGCCGTT GGTTCGTGGA AGCAGAGGGA 
GCTGTGAAAG CGTATTTGTG GGACAGTAAT GAAGATGTGG TGGAGTGGTT GGAGAGGCAG 
CTGGCAGAGG AAGAAGGCGC CAGATCCGTC ATCGACGAGA ATATCAAATA TATTCGCAGA 
GATCACATCC TCAAACAGAT CCGCAGCCTG GTTCAAGCTA ACCCTGAGGT GGCTATGGAC 
TCCATTGTAC ACATGACCCA GCACATTTCC CCCACACAGC GCGCCGAAGT CGTCCGCATC 
CTCTCCACCA TGGACACCCC AGCCTCCTCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

acaca ( XM_017356109.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211598.1 Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X6, mRNA.

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CloneID	ODa03022
Clone ID Related Accession (Same CDS sequence)	XM_017356109.1 , XM_017356110.1 , XM_017356109.2 , NM_001271308.1
Accession Version	XM_017356109.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	acetyl-CoA carboxylase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356109.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGA TTGAGGAATC TGTGCGCTCC ATGGTCATGC GCTACGGCAG TCGCTTGTGG 
AAGCTCCGTG TGCTTCAAGC CGAGCTGAAG ATCAACATCC GATTGACTCC CACCGGCAAA 
CAGATCCCCA TCCGCCTCTT CCTGACCAAC GAGAGTGGCT ACTACCTGGA CATCAGCCTG 
TACAAGGAAG TCACTGACTC CCGTACAGGA CAGGTGGGGC ACAAAGACCG ACAGATCATG 
TTCCAGGCAT ATGGAGATAA GCAGGGTCCT CTTCATGGCA TGCTCATCAA CACACCCTAC 
GTCACAAAAG ACCTGCTGCA GTCCAAACGC TTCCAGGCAC AGAGCCTCGG CACTACCTAT 
GTTTACGACT TCCCAGAAAT GTTTCGCCAG GCTTTAAGGA AGCTGTGGCA ATCCATGGAT 
GCACACGCCA ACTTGCCCAA GTGCCCTCTT CCATCAGAAC TGCTCACCTT CACAGAGCTG 
GTTCTGGATT CTCAGGGTCA GCTGGTGCAG ATGAACCGCC TACCTGGAGG GAATGAGATT 
GGCATGGTAG CATGGCGAAT GACCCTCCGG ACCCCTGAGT ATCCAGCCGG CCGTGAGATT 
ATAGTGATCA GCAATGACAT CACACATAAG ATTGGCTCGT TTGGGCCACA AGAAGATGTT 
CTGTTCCAGC AAGCTTCAGA AATGGCAAGA GAAAGTGGCA TTCCACGGAT CTATATTGCT 
GCCAACAGCG GAGCTCGTAT AGGCCTGGCA GAGGAGATCA GACACATGTT TCATGTGGCC 
TGGCAGGATC CGGTGGACCC ATACAAGGGT TTTAAGTACC TATACCTCAC ACCACAGGAC 
TATAAGAAGG TGTCTGCTCT GAACTCAGTC CACTGTGAAC ATGTGGAGGA TGAGGGGGAA 
TCCAGGTATA AAATCACTGA TATCATTGGG AAAGAGGAAG GGTTGGGTGT GGAGAACTTG 
AGAGGTTCTG GCATGATCGC AGGTGAATCG TCACTGGCGT ATGAGGATAT TATCACCATG 
AATCTGGTGA CATGCAGGGC CATTGGAATC GGAGCGTACT TGGTGAGACT GGGCCAAAGA 
ACCATACAGG TGGAAAACTC TCATATCATC CTGACTGGAG CCGGAGCTCT CAATAAGGTT 
CTCGGGCGTG AGGTGTACAC TTCCAATAAC CAACTGGGTG GAGTACAGAT CATGCACAAT 
AACGGAGTCA CACACACCAA TGTGTGCGAT GATTTTGAGG GAGTGTATAC ATTGCTGCAC 
TGGCTGTCTT ACATGCCAAA GAATATGTCC AGCCCTGTGC CGATACTCTC TGCTAAAGAT 
CCCATTGATC GAGCGATAGA GTTTGTCCCA ACCAAGGCAC CTTATGACCC ACGCTGGATG 
CTAGCTGGAC GTCCCAATCA GAACATTAAG GGTGCATGGG TGAGTGGGTT CTTCGACCAG 
GGCTCTTTTC TGGAGATCAT GCAGCCATGG GCACAGAGTG TTGTCGTAGG ACGGGCCAGG 
TTGGGTGGGA TCCCAACTGG AGTAGTTGCC GTGGAAACCC GATCAGTGGA GCTTTCAATC 
CCAGCAGACC CAGCTAATCT GGACTCGGAA GCCAAGATAA TCCAGCAGGC TGGGCAAGTG 
TGGTTCCCTG ATTCTGCTTT CAAAACTGCA CAAGCCATTA AAGACTTCAA TAGAGAGGGA 
CTTCCGCTTA TAGTGTTTGC CAACTGGAGA GGTTTTTCTG GAGGCATGAA AGACATGTAT 
GACCAGGTGC TGAAGTTTGG AGCGTATATA GTGGACGGTC TGAGAGAGTA CAGACAGCCG 
GTTCTGGTGT ATATCCCACC ACAGGCGGAG CTCCGGGGAG GATCATGGGT TGTCATAGAC 
CCTACTATCA ATCCTCGCCA CATGGAAATG TATGCAGACA AAGACAGCCG TGGCGGTGTG 
CTGGAGCCTG AAGGCACAGT GGAGATCAAG TTCCGCAAAA AAGACCTGGT GAAGACCATG 
CGACGGGTGG ACCCTGTGTA CATGGGGCTT GCAGAAAGAC TGGGAACCCC AGAGTTGAGT 
GTGTCTGAGC GTAAAGAGCT GGAGAGTAAA CTAAAAGAGA GAGAAGAGTT CCTGCTGCCC 
ATATATCATC AAGTAGCTGT TCAGTTCGCT GACCTTCATG ACACACCGGG CCGCATGCAG 
GAGAAAGGAG TCATCACTGA TATCCTTGAG TGGAGCACGT CTCGGCAGTT TTTCTACTGG 
CGTTTGCGGA GGTTATTGCT GGAGGAAACG GTGAAGAGGA AGATCCAGTG TGCTAACAGT 
GAGCTGACGG ACGGACAGGT TCAGGCTATG CTTCGCCGTT GGTTCGTGGA AGCAGAGGGA 
GCTGTGAAAG CGTATTTGTG GGACAGTAAT GAAGATGTGG TGGAGTGGTT GGAGAGGCAG 
CTGGCAGAGG AAGAAGGCGC CAGATCCGTC ATCGACGAGA ATATCAAATA TATTCGCAGA 
GATCACATCC TCAAACAGAT CCGCAGCCTG GTTCAAGCTA ACCCTGAGGT GGCTATGGAC 
TCCATTGTAC ACATGACCCA GCACATTTCC CCCACACAGC GCGCCGAAGT CGTCCGCATC 
CTCTCCACCA TGGACACCCC AGCCTCCTCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211598.1
CDS	237..7409
Translation	MAEQDSTEKKLPAVAALHSHFIVGSVSEENSEDETAGKLDLQLEDNPRSLSPSSVSSDSTYEMGFDSLDGPLHNMRPSMSGLHLVKQGRDRRRIDLQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWAYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGTNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLHKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGTGLTVEWTENDQKKGIINVPTELYEQGCVHDVEAGLKAAEQVGFPVMVKASEGGGGKGIRKVNCAEDFPNLYRQVQAEVPGSPIFVMQLAKHARHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATSDVFEDMEKCAVKLAKMVGYVSAGTVEYLYSQDSSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLHRIKDIRVLYGMQPWGDSPIDFDGLSTTPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQHNSIDTGWLDRLISEKMQAERPDTMLGVVSGALHVADVNFRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGTKYVLKVTRQSPNSYVVIMNCSSVEVDVHRLSDGGMLLSYDGSSYTTYMKEEVDRYRIIIGNKTCVFEKENDPSVLRSPSAGKLIQYTVEDGGHVFAGQCYAEIEVMKMVMTLTASESGCIHYVKRAGAVLEPGCIIGKLQLDDPSRVQQAELYTGTLPSIQSVALRGEKLHRVFHSTLGHLVHIMNGYCLPEPFFSAKLKEWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPAVEKAIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQKYRSGIRGHMKAVVMDLLRQYLRVEVQFQHGHYDKCVFALREENKVDMANVLNYIFSHAQVTKKNCLVTMLIDQLCGRDPTLTDELMAILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCIVEFQFMLPTSHPNRGNIPTLNRKLSAPLQDYKPPANTANTSGATEAASDSAESEDRMSFSSNLNHYGMVHVASVSDVLLDTSFTPPCQRMGAMVSFRSFQEFTRNIKDVLSCFSDSPPSTPTFPEGGNPVLYREEDSKSIQDEPIHILNVAIKTDSDIDDDGLAAMFREFTQSKKSLLFDHGIRRLTFLVAQKDFRKQINCEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFALTAIPCANHKMHLYLGAARVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLHNEAERLLLEAMDELEVAFNNTTVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKQIPIRLFLTNESGYYLDISLYKEVTDSRTGQVGHKDRQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYVYDFPEMFRQALRKLWQSMDAHANLPKCPLPSELLTFTELVLDSQGQLVQMNRLPGGNEIGMVAWRMTLRTPEYPAGREIIVISNDITHKIGSFGPQEDVLFQQASEMARESGIPRIYIAANSGARIGLAEEIRHMFHVAWQDPVDPYKGFKYLYLTPQDYKKVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRGSGMIAGESSLAYEDIITMNLVTCRAIGIGAYLVRLGQRTIQVENSHIILTGAGALNKVLGREVYTSNNQLGGVQIMHNNGVTHTNVCDDFEGVYTLLHWLSYMPKNMSSPVPILSAKDPIDRAIEFVPTKAPYDPRWMLAGRPNQNIKGAWVSGFFDQGSFLEIMQPWAQSVVVGRARLGGIPTGVVAVETRSVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTAQAIKDFNREGLPLIVFANWRGFSGGMKDMYDQVLKFGAYIVDGLREYRQPVLVYIPPQAELRGGSWVVIDPTINPRHMEMYADKDSRGGVLEPEGTVEIKFRKKDLVKTMRRVDPVYMGLAERLGTPELSVSERKELESKLKEREEFLLPIYHQVAVQFADLHDTPGRMQEKGVITDILEWSTSRQFFYWRLRRLLLEETVKRKIQCANSELTDGQVQAMLRRWFVEAEGAVKAYLWDSNEDVVEWLERQLAEEEGARSVIDENIKYIRRDHILKQIRSLVQANPEVAMDSIVHMTQHISPTQRAEVVRILSTMDTPASS

Target ORF information:

RefSeq Version	XM_017356109.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acetyl-CoA carboxylase alpha (acaca), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356109.2

ORF Insert Sequence:

ATGGCAGAGC AAGACTCCAC CGAGAAGAAA CTTCCAGCTG TGGCTGCGCT GCACTCTCAC 
TTCATCGTGG GCTCTGTGTC TGAGGAAAAC TCTGAGGATG AGACCGCAGG GAAGCTGGAC 
CTGCAGCTGG AGGACAATCC ACGCTCCCTT TCCCCCTCCT CCGTCAGTTC AGATAGCACT 
TATGAGATGG GCTTCGACAG CCTGGACGGA CCCTTGCACA ATATGAGGCC TAGCATGTCA 
GGACTTCACC TTGTGAAGCA GGGTAGGGAC CGCAGACGTA TCGACCTGCA GAGGGATTTT 
ACTGTTGCTT CTCCAGCTGA GTTTGTCACC CGCTTCGGTG GAAACAAAGT TATTGAGAAG 
GTCCTTATTG CGAACAATGG CATTGCTGCA GTCAAGTGCA TGCGCTCTAT CCGACGCTGG 
GCTTACGAAA TGTTTCGGAA TGAACGGGCC ATACGCTTTG TTGTCATGGT GACCCCAGAG 
GACCTTAAAG CTAATGCAGA GTACATCAAG ATGGCAGATC ACTATGTGCC TGTGCCAGGA 
GGGACAAACA ACAACAACTA CGCTAATGTG GAACTCATCT TGGACATTGC TAAACGAATT 
CCTGTTCAAG CTGTGTGGGC TGGCTGGGGT CATGCCTCTG AAAATCCCAA ACTTCCTGAA 
TTGCTGCACA AGAACGGCAT CGCCTTCATG GGTCCTCCAA GTCAGGCTAT GTGGGCTCTC 
GGGGATAAGA TCGCTTCCTC TATTGTGGCC CAGACAGCAG GCATTCCCAC ACTACCCTGG 
AGTGGAACAG GACTGACTGT AGAGTGGACA GAGAATGACC AGAAGAAAGG GATTATTAAT 
GTTCCTACTG AGCTATACGA GCAAGGCTGT GTGCACGACG TGGAGGCTGG ATTGAAGGCT 
GCTGAACAAG TGGGCTTCCC TGTGATGGTG AAGGCATCTG AGGGCGGAGG AGGAAAAGGG 
ATCCGGAAAG TCAACTGTGC AGAAGACTTC CCCAACCTCT ATAGACAGGT TCAAGCTGAG 
GTTCCAGGTT CCCCGATTTT TGTCATGCAA TTGGCCAAAC ATGCTCGTCA TCTGGAGGTT 
CAGATCTTGG CTGATCAGTA CGGTAATGCC ATCTCTCTGT TCGGCCGTGA TTGCTCCGTG 
CAGCGCAGAC ACCAGAAGAT CATTGAAGAA GCCCCGGCTA CTATCGCTAC ATCAGACGTG 
TTTGAAGATA TGGAGAAGTG TGCTGTGAAG TTGGCGAAGA TGGTGGGCTA TGTGAGTGCG 
GGTACAGTGG AGTATCTGTA CAGTCAGGAC AGCAGCTTTT ACTTCCTGGA GCTCAATCCA 
CGTCTTCAAG TGGAACATCC TTGTACTGAG ATGGTGGCTG ACGTTAACCT GCCAGCAGCT 
CAACTGCAGA TCGCTATGGG CATCCCACTT CACCGTATTA AAGACATCAG AGTGCTGTAT 
GGGATGCAGC CATGGGGAGA TTCCCCCATT GACTTTGATG GACTCTCTAC CACCCCCTGT 
CCACGAGGAC ACGTCATCGC AGCTCGAATT ACCAGTGAGA ACCCAGATGA GGGTTTTAAA 
CCCAGCTCTG GAACTGTCCA GGAATTAAAT TTCCGCAGTA ACAAGAATGT GTGGGGCTAC 
TTCAGCGTGG CGGCAGCTGG AGGACTGCAT GAGTTTGCAG ACTCTCAGTT TGGCCACTGC 
TTCTCCTGGG GAGAGAACCG TGAGGAAGCC ATCTCAAACA TGGTGGTGGC CCTCAAGGAG 
CTGTCCATCA GAGGAGACTT CAGGACTACA GTGGAGTATC TCATAAAGCT GCTTGAAACG 
GAGAGTTTCC AGCACAACAG CATTGACACA GGATGGCTGG ACAGGCTCAT CTCTGAGAAG 
ATGCAGGCAG AGCGTCCTGA TACTATGCTG GGGGTAGTAA GTGGAGCTCT GCATGTGGCT 
GATGTTAACT TCAGGAACAG TGTGTCCAAC TTCCTGCACT CACTGGAGAG AGGGCAGGTT 
TTACCAGCAC ACACGCTGCT GAACACGGTG GATGTGGAGC TTATCTACGA AGGCACCAAG 
TATGTGCTGA AGGTGACCAG GCAGTCTCCT AACTCTTATG TGGTCATCAT GAATTGCTCT 
TCGGTAGAGG TGGATGTTCA TCGCTTGAGT GACGGTGGTA TGTTGCTCTC GTATGATGGC 
AGCAGCTACA CCACCTACAT GAAGGAGGAG GTAGACAGGT ATCGCATCAT TATCGGGAAT 
AAGACGTGTG TGTTTGAAAA GGAGAACGAC CCTTCAGTCC TTCGCTCTCC TTCTGCTGGG 
AAACTCATCC AGTATACAGT GGAGGATGGA GGACATGTGT TTGCTGGACA ATGTTATGCA 
GAGATAGAGG TGATGAAGAT GGTGATGACT CTGACGGCCT CAGAGTCTGG CTGTATTCAC 
TATGTGAAGA GAGCAGGTGC AGTGCTGGAG CCAGGTTGCA TCATTGGCAA ACTGCAGCTA 
GATGACCCCA GCAGAGTACA GCAGGCGGAG TTGTATACAG GAACTTTACC CAGCATTCAG 
TCTGTGGCTC TCAGGGGAGA GAAGCTTCAC AGAGTCTTCC ACAGCACTCT GGGTCACTTA 
GTGCACATTA TGAACGGCTA CTGTCTGCCA GAGCCTTTCT TCAGTGCCAA GCTGAAAGAA 
TGGGTGGAGC GTCTTATGAA GACTCTAAGG GACCCCTCCC TACCCCTGCT GGAGCTTCAG 
GACATCATGA CCAGTGTTTC AGGCCGAATC CCTCCTGCGG TGGAGAAGGC CATCAAAAAA 
GAAATGGCTC AGTATGCCAG CAACATCACC TCTGTCCTCT GCCAGTTTCC CAGCCAGCAG 
ATTGCCAATA TACTGGACAG TCATGCTGCC ACGCTGAACA GGAAGTCAGA GAGAGAGGTC 
TTCTTCATGA ACACACAGAG CATCGTACAG CTGGTTCAGA AGTACCGCAG TGGCATCAGA 
GGACATATGA AAGCAGTGGT GATGGATCTC CTCAGGCAGT ATTTGAGAGT GGAGGTTCAG 
TTTCAGCATG GTCACTATGA CAAGTGTGTA TTTGCACTAC GAGAGGAAAA CAAAGTAGAC 
ATGGCCAATG TGCTCAACTA CATATTCTCT CATGCTCAAG TCACAAAGAA GAACTGTCTG 
GTCACCATGC TGATTGATCA GCTATGTGGG CGAGACCCGA CCCTGACGGA CGAGCTGATG 
GCCATACTCA CAGAGCTCAC ACAGCTCAGC AAGACCACCA ATGCCAAGGT GGCATTGCGG 
GCACGGCAGG TTCTCATCGC TTCTCACCTT CCCTCATATG AGCTGCGACA CAATCAGGTG 
GAATCCATTT TCCTGTCTGC CATCGACATG TATGGACACC AGTTCTGCAT TGAGAACCTG 
CAGAAACTGA TCCTGTCAGA AACATCCATC TTTGATGTGC TGCCAAATTT CTTTTACCAT 
AGTAATCAGG TGGTACGGAT GGCTGCTCTC GAGGTGTATG TTCGTAGAGC TTACATCGCT 
TATGAACTCA ACAGCGTCCA GCACCGTCAG CTGAAGGACA ACACCTGCAT CGTGGAGTTC 
CAGTTCATGC TGCCCACATC CCACCCCAAC AGAGGGAACA TCCCCACTCT AAACAGGAAA 
CTGTCTGCCC CACTGCAGGA CTACAAACCT CCAGCTAACA CAGCTAACAC TAGTGGAGCC 
ACAGAGGCAG CTAGTGACTC TGCTGAGAGT GAGGACAGAA TGTCCTTTTC CTCAAACCTG 
AACCACTACG GCATGGTCCA CGTGGCCAGC GTTAGTGATG TGCTCCTAGA CACCTCCTTT 
ACCCCACCCT GTCAGCGCAT GGGAGCCATG GTTTCCTTCC GCTCTTTCCA GGAGTTCACC 
AGGAACATAA AGGATGTTCT GAGCTGTTTC TCAGACTCTC CTCCATCAAC TCCAACCTTT 
CCTGAAGGGG GGAACCCTGT GCTGTATAGA GAGGAGGACA GCAAGAGCAT TCAAGATGAG 
CCCATTCATA TTCTTAATGT GGCAATAAAG ACGGACAGTG ATATTGATGA TGATGGGCTG 
GCGGCCATGT TTCGTGAGTT TACACAGTCT AAGAAATCTC TGCTGTTTGA CCACGGCATC 
CGAAGACTTA CCTTCCTGGT TGCTCAGAAG GATTTCAGAA AGCAAATCAA CTGTGAGGTG 
GACCAGAGGT TCCATAGAGA ATTCCCCAAA TTTTTTACAT TCCGGGCTCG AGACAAGTTT 
GAGGAAGACC GTATCTACAG ACACTTGGAA CCTGCATTAG CTTTCCAGCT GGAGCTCAAC 
CGCATGCGTA ATTTTGCACT GACAGCCATC CCCTGTGCCA ATCATAAGAT GCACCTGTAC 
TTGGGAGCTG CCCGTGTGGA AGTAGGCACT GAGGTCACAG ATTACCGCTT CTTTGTGCGG 
GCAATCATCC GTCACTCAGA TTTGGTCACC AAGGAAGCAT CATTTGAGTA CCTGCACAAC 
GAGGCAGAAC GCTTGCTGCT GGAGGCCATG GATGAACTGG AAGTGGCTTT CAACAACACC 
ACTGTGCGAA CCGACTGCAA TCACATCTTC CTCAACTTCG TTCCAACAGT CATCATGGAC 
CCTTCAAAGA TTGAGGAATC TGTGCGCTCC ATGGTCATGC GCTACGGCAG TCGCTTGTGG 
AAGCTCCGTG TGCTTCAAGC CGAGCTGAAG ATCAACATCC GATTGACTCC CACCGGCAAA 
CAGATCCCCA TCCGCCTCTT CCTGACCAAC GAGAGTGGCT ACTACCTGGA CATCAGCCTG 
TACAAGGAAG TCACTGACTC CCGTACAGGA CAGGTGGGGC ACAAAGACCG ACAGATCATG 
TTCCAGGCAT ATGGAGATAA GCAGGGTCCT CTTCATGGCA TGCTCATCAA CACACCCTAC 
GTCACAAAAG ACCTGCTGCA GTCCAAACGC TTCCAGGCAC AGAGCCTCGG CACTACCTAT 
GTTTACGACT TCCCAGAAAT GTTTCGCCAG GCTTTAAGGA AGCTGTGGCA ATCCATGGAT 
GCACACGCCA ACTTGCCCAA GTGCCCTCTT CCATCAGAAC TGCTCACCTT CACAGAGCTG 
GTTCTGGATT CTCAGGGTCA GCTGGTGCAG ATGAACCGCC TACCTGGAGG GAATGAGATT 
GGCATGGTAG CATGGCGAAT GACCCTCCGG ACCCCTGAGT ATCCAGCCGG CCGTGAGATT 
ATAGTGATCA GCAATGACAT CACACATAAG ATTGGCTCGT TTGGGCCACA AGAAGATGTT 
CTGTTCCAGC AAGCTTCAGA AATGGCAAGA GAAAGTGGCA TTCCACGGAT CTATATTGCT 
GCCAACAGCG GAGCTCGTAT AGGCCTGGCA GAGGAGATCA GACACATGTT TCATGTGGCC 
TGGCAGGATC CGGTGGACCC ATACAAGGGT TTTAAGTACC TATACCTCAC ACCACAGGAC 
TATAAGAAGG TGTCTGCTCT GAACTCAGTC CACTGTGAAC ATGTGGAGGA TGAGGGGGAA 
TCCAGGTATA AAATCACTGA TATCATTGGG AAAGAGGAAG GGTTGGGTGT GGAGAACTTG 
AGAGGTTCTG GCATGATCGC AGGTGAATCG TCACTGGCGT ATGAGGATAT TATCACCATG 
AATCTGGTGA CATGCAGGGC CATTGGAATC GGAGCGTACT TGGTGAGACT GGGCCAAAGA 
ACCATACAGG TGGAAAACTC TCATATCATC CTGACTGGAG CCGGAGCTCT CAATAAGGTT 
CTCGGGCGTG AGGTGTACAC TTCCAATAAC CAACTGGGTG GAGTACAGAT CATGCACAAT 
AACGGAGTCA CACACACCAA TGTGTGCGAT GATTTTGAGG GAGTGTATAC ATTGCTGCAC 
TGGCTGTCTT ACATGCCAAA GAATATGTCC AGCCCTGTGC CGATACTCTC TGCTAAAGAT 
CCCATTGATC GAGCGATAGA GTTTGTCCCA ACCAAGGCAC CTTATGACCC ACGCTGGATG 
CTAGCTGGAC GTCCCAATCA GAACATTAAG GGTGCATGGG TGAGTGGGTT CTTCGACCAG 
GGCTCTTTTC TGGAGATCAT GCAGCCATGG GCACAGAGTG TTGTCGTAGG ACGGGCCAGG 
TTGGGTGGGA TCCCAACTGG AGTAGTTGCC GTGGAAACCC GATCAGTGGA GCTTTCAATC 
CCAGCAGACC CAGCTAATCT GGACTCGGAA GCCAAGATAA TCCAGCAGGC TGGGCAAGTG 
TGGTTCCCTG ATTCTGCTTT CAAAACTGCA CAAGCCATTA AAGACTTCAA TAGAGAGGGA 
CTTCCGCTTA TAGTGTTTGC CAACTGGAGA GGTTTTTCTG GAGGCATGAA AGACATGTAT 
GACCAGGTGC TGAAGTTTGG AGCGTATATA GTGGACGGTC TGAGAGAGTA CAGACAGCCG 
GTTCTGGTGT ATATCCCACC ACAGGCGGAG CTCCGGGGAG GATCATGGGT TGTCATAGAC 
CCTACTATCA ATCCTCGCCA CATGGAAATG TATGCAGACA AAGACAGCCG TGGCGGTGTG 
CTGGAGCCTG AAGGCACAGT GGAGATCAAG TTCCGCAAAA AAGACCTGGT GAAGACCATG 
CGACGGGTGG ACCCTGTGTA CATGGGGCTT GCAGAAAGAC TGGGAACCCC AGAGTTGAGT 
GTGTCTGAGC GTAAAGAGCT GGAGAGTAAA CTAAAAGAGA GAGAAGAGTT CCTGCTGCCC 
ATATATCATC AAGTAGCTGT TCAGTTCGCT GACCTTCATG ACACACCGGG CCGCATGCAG 
GAGAAAGGAG TCATCACTGA TATCCTTGAG TGGAGCACGT CTCGGCAGTT TTTCTACTGG 
CGTTTGCGGA GGTTATTGCT GGAGGAAACG GTGAAGAGGA AGATCCAGTG TGCTAACAGT 
GAGCTGACGG ACGGACAGGT TCAGGCTATG CTTCGCCGTT GGTTCGTGGA AGCAGAGGGA 
GCTGTGAAAG CGTATTTGTG GGACAGTAAT GAAGATGTGG TGGAGTGGTT GGAGAGGCAG 
CTGGCAGAGG AAGAAGGCGC CAGATCCGTC ATCGACGAGA ATATCAAATA TATTCGCAGA 
GATCACATCC TCAAACAGAT CCGCAGCCTG GTTCAAGCTA ACCCTGAGGT GGCTATGGAC 
TCCATTGTAC ACATGACCCA GCACATTTCC CCCACACAGC GCGCCGAAGT CGTCCGCATC 
CTCTCCACCA TGGACACCCC AGCCTCCTCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	acaca
Entrez Gene ID	559403
Full Name	acetyl-CoA carboxylase alpha
Synonyms	acc,fj43d01,im:7138837,si:ch211-199d18.1,wu:fj43d01
General protein information	Preferred Names acetyl-CoA carboxylase alpha Names acetyl-CoA carboxylase 1 acetyl-Coenzyme A carboxylase alpha
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Canis lupus familiaris (dog)	ACACA	XP_005624832.1
Bos taurus (cattle)	ACACA	NP_776649.1
Mus musculus (house mouse)	Acaca	NP_579938.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005175	XP_314071.4
Schizosaccharomyces pombe (fission yeast)	cut6	NP_593271.1
Arabidopsis thaliana (thale cress)	ACC1	NP_174849.2
	ACACA	XP_001111823.1
Rattus norvegicus (Norway rat)	Acaca	NP_071529.1
Magnaporthe oryzae (rice blast fungus)	MGG_07613	XP_003711534.1
Drosophila melanogaster (fruit fly)	ACC	NP_724636.1
Saccharomyces cerevisiae (baker's yeast)	ACC1	NP_014413.1
Eremothecium gossypii	AGOS_AAR071W	NP_982612.1
Neurospora crassa	NCU08535	XP_963017.1
Pan troglodytes (chimpanzee)	ACACA	XP_511428.3
Gallus gallus (chicken)	ACACA	NP_990836.1
Arabidopsis thaliana (thale cress)	ACC2	NP_174850.4
Homo sapiens (human)	ACACA	NP_942131.1
Danio rerio (zebrafish)	acaca	NP_001258237.1
Kluyveromyces lactis	KLLA0F06072g	XP_455355.1
Xenopus tropicalis (tropical clawed frog)	acaca	XP_004911956.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

acaca cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

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