ACC1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACC1
Entrez Gene ID	840521
Full Name	acetyl-CoA carboxylase 1
Synonyms	AT-ACC1,EMB22,EMBRYO DEFECTIVE 22,GK,GLOSSYHEAD 1,GSD1,GURKE,PAS3,PASTICCINO 3,SENSITIVE TO FREEZING 3,SFR3,acetyl-CoA carboxylase 1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001198214.2	NP_001185143.1	acetyl-CoA carboxylase 1
NM_103313.4	NP_174849.2	acetyl-CoA carboxylase 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ACACA	XP_005624832.1
Bos taurus (cattle)	ACACA	NP_776649.1
Mus musculus (house mouse)	Acaca	NP_579938.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005175	XP_314071.4
Schizosaccharomyces pombe (fission yeast)	cut6	NP_593271.1
Arabidopsis thaliana (thale cress)	ACC1	NP_174849.2
	ACACA	XP_001111823.1
Rattus norvegicus (Norway rat)	Acaca	NP_071529.1
Magnaporthe oryzae (rice blast fungus)	MGG_07613	XP_003711534.1
Drosophila melanogaster (fruit fly)	ACC	NP_724636.1
Saccharomyces cerevisiae (baker's yeast)	ACC1	NP_014413.1
Eremothecium gossypii	AGOS_AAR071W	NP_982612.1
Neurospora crassa	NCU08535	XP_963017.1
Pan troglodytes (chimpanzee)	ACACA	XP_511428.3
Gallus gallus (chicken)	ACACA	NP_990836.1
Arabidopsis thaliana (thale cress)	ACC2	NP_174850.4
Homo sapiens (human)	ACACA	NP_942131.1
Danio rerio (zebrafish)	acaca	NP_001258237.1
Kluyveromyces lactis	KLLA0F06072g	XP_455355.1
Xenopus tropicalis (tropical clawed frog)	acaca	XP_004911956.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

sensitive to freezing3(sfr3) is a missense mutation in aminoglycoside N1-acetyltransferase. Freezing sensitivity of sfr3 appears to be due to cuticular deficiencies that develop during cold acclimation.
Title: The sensitive to freezing3 mutation of Arabidopsis thaliana is a cold-sensitive allele of homomeric acetyl-CoA carboxylase that results in cold-induced cuticle deficiencies.

The gsd1 locus was mapped to chromosome 1, and the causal gene was identified as a new allele of Acetyl-Coenzyme A Carboxylase1 (ACC1), a gene encoding the main enzyme in cytosolic malonyl-coenzyme A synthesis.
Title: The glossyhead1 allele of ACC1 reveals a principal role for multidomain acetyl-coenzyme A carboxylase in the biosynthesis of cuticular waxes by Arabidopsis.

Data suggest that the lack of cytosolic malonyl-CoA is likely to be the initial factor leading to abnormal development in acetyl-CoA carboxylase 1 mutants.
Title: gurke and pasticcino3 mutants affected in embryo development are impaired in acetyl-CoA carboxylase.

The following ACC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb25573	NM_001198214.2 Latest version!	Arabidopsis thaliana acetyl-CoA carboxylase 1 (ACC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OAb25573	NM_103313.4 Latest version!	Arabidopsis thaliana acetyl-CoA carboxylase 1 (ACC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACC1 ( NM_001198214.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001185143.1 Arabidopsis thaliana acetyl-CoA carboxylase 1 (ACC1), mRNA.

Price & Availability↑

CloneID	OAb25573
Clone ID Related Accession (Same CDS sequence)	NM_001198214.2 , NM_103313.4
Accession Version	NM_001198214.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	acetyl-CoA carboxylase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_001198214.1.

ATGGCTGGCT CGGTTAACGG GAATCATAGT GCTGTAGGAC CTGGTATAAA TTATGAGACG 
GTGTCTCAAG TGGATGAGTT CTGTAAAGCA CTTAGAGGGA AAAGGCCGAT CCATAGTATT 
TTGATAGCTA ACAATGGAAT GGCGGCTGTG AAGTTTATAC GTAGTGTCAG AACATGGGCT 
TATGAAACAT TTGGTACGGA AAAAGCCATA TTGTTGGTGG GGATGGCAAC CCCTGAAGAC 
ATGCGGATCA ATGCGGAGCA TATCAGAATC GCTGATCAGT TTGTTGAGGT TCCCGGAGGA 
ACCAACAATA ACAATTATGC TAACGTTCAG CTGATTGTGG AGATGGCTGA AGTAACACGC 
GTGGATGCAG TTTGGCCTGG TTGGGGTCAT GCATCTGAAA ACCCCGAATT ACCTGATGCC 
CTAGATGCAA AAGGAATCAT ATTTCTTGGT CCTCCAGCAT CTTCAATGGC AGCACTGGGA 
GATAAGATTG GTTCTTCGTT GATTGCACAA GCTGCTGATG TACCCACTCT GCCATGGAGT 
GGTTCCCATG TTAAAATACC TCCTAATAGC AACTTGGTAA CCATCCCAGA GGAGATCTAC 
CGGCAAGCAT GTGTCTACAC AACTGAAGAA GCGATTGCTA GCTGTCAAGT TGTCGGTTAC 
CCAGCAATGA TCAAAGCATC GTGGGGTGGT GGTGGTAAAG GAATCAGGAA GGTTCATAAT 
GATGATGAGG TTAGGGCTCT ATTCAAGCAA GTTCAGGGTG AGGTCCCAGG CTCACCAATA 
TTCATAATGA AGGTTGCGTC ACAGAGTCGG CATCTAGAGG TCCAGCTGCT CTGTGACAAG 
CATGGAAATG TTTCAGCTCT GCATAGCCGT GATTGTAGCG TCCAGAGAAG ACATCAAAAG 
ATCATAGAGG AGGGTCCAAT TACTGTGGCT CCGCCAGAAA CTGTCAAGAA ACTTGAACAA 
GCAGCTAGAA GGTTGGCTAA GAGTGTTAAC TATGTTGGAG CTGCTACTGT TGAGTATCTC 
TACAGTATGG ACACTGGGGA GTACTACTTC TTAGAGCTTA ACCCTCGCTT ACAGGTTGAG 
CATCCTGTCA CTGAGTGGAT TGCCGAGATA AATCTTCCTG CTGCCCAAGT TGCTGTGGGG 
ATGGGAATTC CTCTCTGGCA AATCCCTGAG ATAAGACGGT TCTATGGAAT AGAACATGGT 
GGAGGTTATG ATTCTTGGCG AAAAACATCT GTTGTAGCCT TCCCTTTTGA TTTTGATAAA 
GCTCAATCTA TAAGGCCAAA AGGTCATTGT GTGGCTGTAC GTGTGACAAG TGAGGATCCT 
GATGACGGGT TCAAACCAAC CAGCGGTAGA GTTCAGGAGT TGAGTTTTAA GAGCAAGCCA 
AATGTGTGGG CGTACTTCTC TGTCAAGTCT GGTGGAGGCA TCCACGAGTT CTCGGATTCC 
CAGTTTGGAC ATGTTTTTGC ATTTGGGGAA TCCAGAGCCC TGGCGATAGC GAATATGGTT 
CTTGGGCTAA AAGAAATTCA GATCCGTGGA GAAATTAGGA CTAACGTTGA CTACACGATC 
GACCTTTTAC ATGCTTCTGA TTACCGTGAT AACAAAATTC ACACTGGTTG GTTGGATAGT 
AGGATTGCTA TGCGGGTCAG AGCTGAGAGG CCTCCATGGT ATCTCTCTGT TGTCGGCGGA 
GCTCTCTATA AAGCATCAGC GACCAGTGCT GCTGTGGTTT CAGATTACGT TGGTTATCTG 
GAGAAGGGGC AAATCCCTCC AAAGCATATA TCTCTTGTAC ATTCTCAAGT GTCTCTGAAT 
ATTGAAGGAA GTAAATATAC GATTGATGTA GTCCGGGGTG GATCAGGAAC CTACAGGCTA 
AGAATGAACA AGTCAGAAGT GGTAGCAGAA ATACACACTC TACGTGATGG AGGTCTGTTG 
ATGCAGTTGG ATGGCAAAAG CCATGTGATA TATGCAGAGG AAGAAGCTGC AGGAACTCGT 
CTTCTCATTG ATGGAAGAAC TTGTTTGCTA CAGAATGACC ACGATCCATC AAAGTTAATG 
GCTGAGACAC CGTGCAAGTT GATGAGGTAT TTGATTTCCG ACAACAGCAA TATTGACGCT 
GATACGCCTT ATGCCGAAGT TGAGGTCATG AAGATGTGCA TGCCACTTCT TTCACCTGCT 
TCAGGAGTTA TCCATTTTAA AATGTCTGAA GGACAAGCCA TGCAGGCTGG TGAACTTATA 
GCCAATCTTG ATCTTGATGA TCCTTCTGCT GTAAGAAAGG CCGAACCCTT CCATGGAAGT 
TTCCCAAGAT TAGGGCTTCC AACTGCAATA TCCGGTAGAG TTCATCAGAG ATGTGCCGCA 
ACATTAAATG CTGCACGCAT GATTCTTGCT GGCTATGAGC ATAAAGTAGA TGAGGTTGTT 
CAAGACTTAC TTAATTGCCT TGATAGCCCT GAACTCCCAT TTCTTCAGTG GCAAGAGTGC 
TTTGCAGTTC TGGCGACACG ACTACCTAAA AATCTCAGGA ACATGCTAGA ATCAAAGTAT 
AGGGAATTTG AGAGTATTTC CAGAAACTCT TTGACCACCG ATTTCCCTGC CAAACTTTTA 
AAAGGCATTC TTGAGGCACA TTTATCTTCT TGTGATGAGA AAGAGAGAGG TGCCCTTGAA 
AGGCTCATTG AACCATTGAT GAGCCTTGCA AAATCTTATG AAGGTGGTAG AGAAAGTCAT 
GCCCGTGTTA TTGTTCATTC TCTCTTTGAA GAATATCTAT CAGTAGAAGA ATTATTCAAT 
GATAACATGC TGGCTGATGT TATAGAACGC ATGCGTCAGC TATACAAGAA AGATCTGTTG 
AAAATTGTGG ATATAGTGCT CTCACACCAG GGCATAAAAA ACAAAAACAA ACTCGTTCTC 
CGGCTCATGG AGCAGCTTGT TTACCCTAAT CCTGCTGCTT ACAGAGATAA ACTTATTCGA 
TTCTCAACAC TTAACCATAC TAACTACTCT GAGTTGGCGC TCAAGGCGAG TCAATTACTT 
GAACAGACCA AACTAAGTGA GCTTCGTTCA AACATTGCTA GAAGCCTTTC AGAGTTAGAA 
ATGTTTACAG AGGACGGAGA AAATATGGAT ACTCCCAAGA GGAAAAGTGC CATTAATGAA 
AGAATAGAAG ATCTTGTAAG CGCATCTTTA GCTGTTGAAG ACGCTCTCGT GGGACTATTT 
GACCATAGCG ATCACACACT TCAAAGACGG GTTGTTGAGA CTTATATTCG CAGATTATAC 
CAGCCCTACG TCGTTAAAGA TAGCGTGAGG ATGCAGTGGC ACCGTTCTGG TCTTCTTGCT 
TCCTGGGAGT TCCTAGAGGA GCATATGGAA AGAAAAAACA TTGGCTTAGA CGATCCCGAC 
ACATCTGAAA AAGGATTGGT TGAGAAGCGT AGTAAGAGAA AATGGGGGGC TATGGTTATA 
ATCAAATCTT TGCAGTTTCT TCCAAGTATA ATAAGTGCAG CATTGAGAGA AACAAAGCAC 
AACGACTATG AAACTGCCGG AGCTCCTTTA TCTGGCAATA TGATGCACAT TGCTATTGTG 
GGCATCAACA ACCAGATGAG TCTGCTTCAG GACAGTGGGG ATGAAGACCA AGCTCAGGAA 
AGAGTAAACA AGTTGGCCAA AATTCTTAAA GAGGAAGAAG TGAGTTCAAG CCTCTGTTCT 
GCCGGTGTTG GTGTAATCAG CTGTATAATT CAGCGAGATG AAGGACGAAC ACCCATGAGA 
CATTCTTTCC ATTGGTCGTT GGAGAAACAG TATTATGTAG AAGAGCCGTT GCTGCGTCAT 
CTTGAACCTC CTCTGTCCAT TTACCTTGAG TTGGATAAGC TGAAAGGATA CTCAAATATA 
CAATATACGC CTTCTCGAGA TCGTCAATGG CATCTGTATA CTGTTACAGA CAAGCCAGTG 
CCAATCAAGA GGATGTTCCT GAGATCTCTT GTTCGACAGG CTACAATGAA CGATGGATTT 
ATATTGCAGC AAGGGCAGGA TAAGCAGCTT AGCCAAACAC TGATCTCCAT GGCGTTTACG 
TCGAAATGTG TTCTGAGGTC TTTGATGGAT GCCATGGAGG AACTGGAACT GAATGCCCAT 
AATGCTGCAA TGAAACCAGA TCACGCACAT ATGTTTCTTT GCATATTGCG TGAGCAGCAG 
ATAGATGATC TTGTGCCTTT CCCCAGGAGA GTTGAAGTGA ATGCGGAGGA TGAAGAAACT 
ACAGTTGAAA TGATCTTAGA AGAAGCAGCA CGAGAGATAC ATAGATCTGT TGGAGTGAGA 
ATGCATAGGT TGGGCGTGTG CGAGTGGGAA GTGCGGCTGT GGTTGGTGTC CTCTGGACTG 
GCATGTGGTG CTTGGAGGGT TGTGGTTGCA AACGTGACAG GCCGTACATG CACTGTCCAC 
ATATACCGAG AAGTTGAAAC TCCTGGAAGA AACAGTTTAA TCTACCACTC AATAACCAAG 
AAGGGACCTT TGCATGAAAC ACCAATCAGT GATCAATATA AGCCCCTGGG ATATCTCGAC 
AGGCAACGTT TAGCAGCAAG GAGGAGTAAC ACTACTTATT GCTATGACTT CCCGTTGGCA 
TTTGGGACAG CCTTGGAACT GTTGTGGGCA TCACAACACC CAGGAGTTAA GAAACCATAT 
AAGGATACTC TGATCAATGT TAAAGAGCTT GTATTCTCAA AACCAGAAGG TTCTTCGGGT 
ACATCTCTAG ATCTGGTTGA AAGACCACCC GGTCTCAACG ACTTTGGAAT GGTTGCCTGG 
TGCCTAGATA TGTCGACCCC AGAGTTTCCT ATGGGGCGGA AACTTCTCGT GATTGCGAAT 
GATGTCACCT TCAAAGCTGG TTCTTTTGGT CCTAGAGAGG ACGCGTTTTT CCTTGCTGTT 
ACTGAACTCG CTTGTGCCAA GAAGCTTCCC TTGATTTACT TGGCAGCAAA TTCTGGTGCC 
CGACTTGGGG TTGCTGAAGA AGTCAAAGCC TGCTTCAAAG TTGGATGGTC GGATGAAATT 
TCCCCTGAGA ATGGTTTTCA GTATATATAC CTAAGCCCTG AAGACCACGA AAGGATTGGA 
TCATCTGTCA TTGCCCATGA AGTAAAGCTC TCTAGTGGGG AAACTAGGTG GGTGATTGAT 
ACGATCGTTG GCAAAGAAGA TGGTATTGGT GTAGAGAACT TAACAGGAAG TGGGGCCATA 
GCGGGTGCTT ACTCAAAGGC ATACAATGAA ACTTTTACTT TAACCTTTGT TAGTGGAAGA 
ACGGTTGGAA TTGGTGCTTA TCTTGCCCGC CTAGGTATGC GGTGCATACA GAGACTTGAT 
CAGCCGATCA TCTTGACTGG CTTCTCTACA CTCAACAAGT TACTTGGGCG TGAGGTCTAT 
AGCTCTCACA TGCAACTGGG TGGCCCGAAA ATCATGGGCA CAAATGGTGT TGTTCATCTT 
ACAGTCTCAG ATGATCTTGA AGGCGTATCA GCAATTCTCA ACTGGCTCAG CTACATTCCT 
GCTTACGTGG GTGGTCCTCT TCCTGTTCTT GCCCCTTTAG ATCCACCGGA GAGAATTGTG 
GAGTATGTCC CAGAGAACTC TTGCGACCCA CGAGCGGCTA TAGCTGGGGT CAAAGACAAT 
ACCGGTAAAT GGCTTGGAGG TATCTTTGAT AAAAATAGTT TCATTGAGAC TCTTGAAGGC 
TGGGCAAGGA CGGTAGTGAC TGGTAGAGCC AAGCTCGGGG GAATACCCGT TGGAGTTGTT 
GCAGTTGAGA CACAGACTGT CATGCAGATC ATCCCAGCCG ATCCTGGACA GCTTGACTCT 
CATGAAAGAG TGGTTCCGCA AGCAGGGCAA GTCTGGTTTC CTGATTCAGC GGCCAAGACT 
GCTCAAGCGC TTATGGATTT CAACCGGGAA GAGCTTCCAT TGTTTATCCT AGCGAACTGG 
AGAGGGTTTT CAGGTGGGCA GAGAGATCTT TTCGAAGGAA TACTTCAGGC AGGTTCAACT 
ATAGTAGAAA ATCTGAGAAC CTATCGTCAG CCAGTGTTTG TGTACATCCC AATGATGGGA 
GAGCTGCGCG GTGGAGCGTG GGTTGTTGTT GACAGCCAGA TAAATTCGGA TTATGTTGAA 
ATGTATGCTG ATGAAACAGC TCGTGGAAAT GTGCTTGAGC CAGAAGGGAC AATAGAGATA 
AAATTTAGAA CAAAAGAGCT ATTAGAGTGC ATGGGAAGGT TGGACCAGAA GCTAATCAGT 
CTGAAAGCAA AACTGCAAGA TGCCAAGCAA AGCGAGGCCT ATGCAAACAT CGAGCTTCTC 
CAGCAACAGA TTAAAGCCCG AGAGAAACAG CTTTTACCAG TTTATATCCA AATCGCCACC 
AAATTTGCAG AACTTCATGA CACTTCCATG AGAATGGCTG CAAAGGGAGT GATCAAAAGT 
GTTGTGGAAT GGAGCGGCTC GCGGTCCTTC TTCTACAAAA AGCTCAATAG GAGAATCGCT 
GAGAGCTCTC TTGTGAAAAA CGTAAGAGAA GCATCTGGAG ACAACTTAGC ATATAAATCT 
TCAATGCGTC TGATTCAGGA TTGGTTCTGC AACTCTGATA TTGCAAAGGG GAAAGAAGAA 
GCTTGGACAG ACGACCAAGT GTTCTTTACA TGGAAGGACA ATGTTAGTAA CTACGAGTTG 
AAGCTGAGCG AGTTGAGAGC GCAGAAACTA CTGAACCAAC TTGCAGAGAT TGGGAATTCC 
TCAGATTTGC AAGCTCTGCC ACAAGGACTT GCTAATCTTC TAAACAAGGT GGAGCCGTCG 
AAAAGAGAAG AGCTGGTGGC TGCTATTCGA AAGGTCTTGG GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185143.1
CDS	422..7186
Translation	MAGSVNGNHSAVGPGINYETVSQVDEFCKALRGKRPIHSILIANNGMAAVKFIRSVRTWAYETFGTEKAILLVGMATPEDMRINAEHIRIADQFVEVPGGTNNNNYANVQLIVEMAEVTRVDAVWPGWGHASENPELPDALDAKGIIFLGPPASSMAALGDKIGSSLIAQAADVPTLPWSGSHVKIPPNSNLVTIPEEIYRQACVYTTEEAIASCQVVGYPAMIKASWGGGGKGIRKVHNDDEVRALFKQVQGEVPGSPIFIMKVASQSRHLEVQLLCDKHGNVSALHSRDCSVQRRHQKIIEEGPITVAPPETVKKLEQAARRLAKSVNYVGAATVEYLYSMDTGEYYFLELNPRLQVEHPVTEWIAEINLPAAQVAVGMGIPLWQIPEIRRFYGIEHGGGYDSWRKTSVVAFPFDFDKAQSIRPKGHCVAVRVTSEDPDDGFKPTSGRVQELSFKSKPNVWAYFSVKSGGGIHEFSDSQFGHVFAFGESRALAIANMVLGLKEIQIRGEIRTNVDYTIDLLHASDYRDNKIHTGWLDSRIAMRVRAERPPWYLSVVGGALYKASATSAAVVSDYVGYLEKGQIPPKHISLVHSQVSLNIEGSKYTIDVVRGGSGTYRLRMNKSEVVAEIHTLRDGGLLMQLDGKSHVIYAEEEAAGTRLLIDGRTCLLQNDHDPSKLMAETPCKLMRYLISDNSNIDADTPYAEVEVMKMCMPLLSPASGVIHFKMSEGQAMQAGELIANLDLDDPSAVRKAEPFHGSFPRLGLPTAISGRVHQRCAATLNAARMILAGYEHKVDEVVQDLLNCLDSPELPFLQWQECFAVLATRLPKNLRNMLESKYREFESISRNSLTTDFPAKLLKGILEAHLSSCDEKERGALERLIEPLMSLAKSYEGGRESHARVIVHSLFEEYLSVEELFNDNMLADVIERMRQLYKKDLLKIVDIVLSHQGIKNKNKLVLRLMEQLVYPNPAAYRDKLIRFSTLNHTNYSELALKASQLLEQTKLSELRSNIARSLSELEMFTEDGENMDTPKRKSAINERIEDLVSASLAVEDALVGLFDHSDHTLQRRVVETYIRRLYQPYVVKDSVRMQWHRSGLLASWEFLEEHMERKNIGLDDPDTSEKGLVEKRSKRKWGAMVIIKSLQFLPSIISAALRETKHNDYETAGAPLSGNMMHIAIVGINNQMSLLQDSGDEDQAQERVNKLAKILKEEEVSSSLCSAGVGVISCIIQRDEGRTPMRHSFHWSLEKQYYVEEPLLRHLEPPLSIYLELDKLKGYSNIQYTPSRDRQWHLYTVTDKPVPIKRMFLRSLVRQATMNDGFILQQGQDKQLSQTLISMAFTSKCVLRSLMDAMEELELNAHNAAMKPDHAHMFLCILREQQIDDLVPFPRRVEVNAEDEETTVEMILEEAAREIHRSVGVRMHRLGVCEWEVRLWLVSSGLACGAWRVVVANVTGRTCTVHIYREVETPGRNSLIYHSITKKGPLHETPISDQYKPLGYLDRQRLAARRSNTTYCYDFPLAFGTALELLWASQHPGVKKPYKDTLINVKELVFSKPEGSSGTSLDLVERPPGLNDFGMVAWCLDMSTPEFPMGRKLLVIANDVTFKAGSFGPREDAFFLAVTELACAKKLPLIYLAANSGARLGVAEEVKACFKVGWSDEISPENGFQYIYLSPEDHERIGSSVIAHEVKLSSGETRWVIDTIVGKEDGIGVENLTGSGAIAGAYSKAYNETFTLTFVSGRTVGIGAYLARLGMRCIQRLDQPIILTGFSTLNKLLGREVYSSHMQLGGPKIMGTNGVVHLTVSDDLEGVSAILNWLSYIPAYVGGPLPVLAPLDPPERIVEYVPENSCDPRAAIAGVKDNTGKWLGGIFDKNSFIETLEGWARTVVTGRAKLGGIPVGVVAVETQTVMQIIPADPGQLDSHERVVPQAGQVWFPDSAAKTAQALMDFNREELPLFILANWRGFSGGQRDLFEGILQAGSTIVENLRTYRQPVFVYIPMMGELRGGAWVVVDSQINSDYVEMYADETARGNVLEPEGTIEIKFRTKELLECMGRLDQKLISLKAKLQDAKQSEAYANIELLQQQIKAREKQLLPVYIQIATKFAELHDTSMRMAAKGVIKSVVEWSGSRSFFYKKLNRRIAESSLVKNVREASGDNLAYKSSMRLIQDWFCNSDIAKGKEEAWTDDQVFFTWKDNVSNYELKLSELRAQKLLNQLAEIGNSSDLQALPQGLANLLNKVEPSKREELVAAIRKVLG

Target ORF information:

RefSeq Version	NM_001198214.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana acetyl-CoA carboxylase 1 (ACC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198214.2

ORF Insert Sequence:

ATGGCTGGCT CGGTTAACGG GAATCATAGT GCTGTAGGAC CTGGTATAAA TTATGAGACG 
GTGTCTCAAG TGGATGAGTT CTGTAAAGCA CTTAGAGGGA AAAGGCCGAT CCATAGTATT 
TTGATAGCTA ACAATGGAAT GGCGGCTGTG AAGTTTATAC GTAGTGTCAG AACATGGGCT 
TATGAAACAT TTGGTACGGA AAAAGCCATA TTGTTGGTGG GGATGGCAAC CCCTGAAGAC 
ATGCGGATCA ATGCGGAGCA TATCAGAATC GCTGATCAGT TTGTTGAGGT TCCCGGAGGA 
ACCAACAATA ACAATTATGC TAACGTTCAG CTGATTGTGG AGATGGCTGA AGTAACACGC 
GTGGATGCAG TTTGGCCTGG TTGGGGTCAT GCATCTGAAA ACCCCGAATT ACCTGATGCC 
CTAGATGCAA AAGGAATCAT ATTTCTTGGT CCTCCAGCAT CTTCAATGGC AGCACTGGGA 
GATAAGATTG GTTCTTCGTT GATTGCACAA GCTGCTGATG TACCCACTCT GCCATGGAGT 
GGTTCCCATG TTAAAATACC TCCTAATAGC AACTTGGTAA CCATCCCAGA GGAGATCTAC 
CGGCAAGCAT GTGTCTACAC AACTGAAGAA GCGATTGCTA GCTGTCAAGT TGTCGGTTAC 
CCAGCAATGA TCAAAGCATC GTGGGGTGGT GGTGGTAAAG GAATCAGGAA GGTTCATAAT 
GATGATGAGG TTAGGGCTCT ATTCAAGCAA GTTCAGGGTG AGGTCCCAGG CTCACCAATA 
TTCATAATGA AGGTTGCGTC ACAGAGTCGG CATCTAGAGG TCCAGCTGCT CTGTGACAAG 
CATGGAAATG TTTCAGCTCT GCATAGCCGT GATTGTAGCG TCCAGAGAAG ACATCAAAAG 
ATCATAGAGG AGGGTCCAAT TACTGTGGCT CCGCCAGAAA CTGTCAAGAA ACTTGAACAA 
GCAGCTAGAA GGTTGGCTAA GAGTGTTAAC TATGTTGGAG CTGCTACTGT TGAGTATCTC 
TACAGTATGG ACACTGGGGA GTACTACTTC TTAGAGCTTA ACCCTCGCTT ACAGGTTGAG 
CATCCTGTCA CTGAGTGGAT TGCCGAGATA AATCTTCCTG CTGCCCAAGT TGCTGTGGGG 
ATGGGAATTC CTCTCTGGCA AATCCCTGAG ATAAGACGGT TCTATGGAAT AGAACATGGT 
GGAGGTTATG ATTCTTGGCG AAAAACATCT GTTGTAGCCT TCCCTTTTGA TTTTGATAAA 
GCTCAATCTA TAAGGCCAAA AGGTCATTGT GTGGCTGTAC GTGTGACAAG TGAGGATCCT 
GATGACGGGT TCAAACCAAC CAGCGGTAGA GTTCAGGAGT TGAGTTTTAA GAGCAAGCCA 
AATGTGTGGG CGTACTTCTC TGTCAAGTCT GGTGGAGGCA TCCACGAGTT CTCGGATTCC 
CAGTTTGGAC ATGTTTTTGC ATTTGGGGAA TCCAGAGCCC TGGCGATAGC GAATATGGTT 
CTTGGGCTAA AAGAAATTCA GATCCGTGGA GAAATTAGGA CTAACGTTGA CTACACGATC 
GACCTTTTAC ATGCTTCTGA TTACCGTGAT AACAAAATTC ACACTGGTTG GTTGGATAGT 
AGGATTGCTA TGCGGGTCAG AGCTGAGAGG CCTCCATGGT ATCTCTCTGT TGTCGGCGGA 
GCTCTCTATA AAGCATCAGC GACCAGTGCT GCTGTGGTTT CAGATTACGT TGGTTATCTG 
GAGAAGGGGC AAATCCCTCC AAAGCATATA TCTCTTGTAC ATTCTCAAGT GTCTCTGAAT 
ATTGAAGGAA GTAAATATAC GATTGATGTA GTCCGGGGTG GATCAGGAAC CTACAGGCTA 
AGAATGAACA AGTCAGAAGT GGTAGCAGAA ATACACACTC TACGTGATGG AGGTCTGTTG 
ATGCAGTTGG ATGGCAAAAG CCATGTGATA TATGCAGAGG AAGAAGCTGC AGGAACTCGT 
CTTCTCATTG ATGGAAGAAC TTGTTTGCTA CAGAATGACC ACGATCCATC AAAGTTAATG 
GCTGAGACAC CGTGCAAGTT GATGAGGTAT TTGATTTCCG ACAACAGCAA TATTGACGCT 
GATACGCCTT ATGCCGAAGT TGAGGTCATG AAGATGTGCA TGCCACTTCT TTCACCTGCT 
TCAGGAGTTA TCCATTTTAA AATGTCTGAA GGACAAGCCA TGCAGGCTGG TGAACTTATA 
GCCAATCTTG ATCTTGATGA TCCTTCTGCT GTAAGAAAGG CCGAACCCTT CCATGGAAGT 
TTCCCAAGAT TAGGGCTTCC AACTGCAATA TCCGGTAGAG TTCATCAGAG ATGTGCCGCA 
ACATTAAATG CTGCACGCAT GATTCTTGCT GGCTATGAGC ATAAAGTAGA TGAGGTTGTT 
CAAGACTTAC TTAATTGCCT TGATAGCCCT GAACTCCCAT TTCTTCAGTG GCAAGAGTGC 
TTTGCAGTTC TGGCGACACG ACTACCTAAA AATCTCAGGA ACATGCTAGA ATCAAAGTAT 
AGGGAATTTG AGAGTATTTC CAGAAACTCT TTGACCACCG ATTTCCCTGC CAAACTTTTA 
AAAGGCATTC TTGAGGCACA TTTATCTTCT TGTGATGAGA AAGAGAGAGG TGCCCTTGAA 
AGGCTCATTG AACCATTGAT GAGCCTTGCA AAATCTTATG AAGGTGGTAG AGAAAGTCAT 
GCCCGTGTTA TTGTTCATTC TCTCTTTGAA GAATATCTAT CAGTAGAAGA ATTATTCAAT 
GATAACATGC TGGCTGATGT TATAGAACGC ATGCGTCAGC TATACAAGAA AGATCTGTTG 
AAAATTGTGG ATATAGTGCT CTCACACCAG GGCATAAAAA ACAAAAACAA ACTCGTTCTC 
CGGCTCATGG AGCAGCTTGT TTACCCTAAT CCTGCTGCTT ACAGAGATAA ACTTATTCGA 
TTCTCAACAC TTAACCATAC TAACTACTCT GAGTTGGCGC TCAAGGCGAG TCAATTACTT 
GAACAGACCA AACTAAGTGA GCTTCGTTCA AACATTGCTA GAAGCCTTTC AGAGTTAGAA 
ATGTTTACAG AGGACGGAGA AAATATGGAT ACTCCCAAGA GGAAAAGTGC CATTAATGAA 
AGAATAGAAG ATCTTGTAAG CGCATCTTTA GCTGTTGAAG ACGCTCTCGT GGGACTATTT 
GACCATAGCG ATCACACACT TCAAAGACGG GTTGTTGAGA CTTATATTCG CAGATTATAC 
CAGCCCTACG TCGTTAAAGA TAGCGTGAGG ATGCAGTGGC ACCGTTCTGG TCTTCTTGCT 
TCCTGGGAGT TCCTAGAGGA GCATATGGAA AGAAAAAACA TTGGCTTAGA CGATCCCGAC 
ACATCTGAAA AAGGATTGGT TGAGAAGCGT AGTAAGAGAA AATGGGGGGC TATGGTTATA 
ATCAAATCTT TGCAGTTTCT TCCAAGTATA ATAAGTGCAG CATTGAGAGA AACAAAGCAC 
AACGACTATG AAACTGCCGG AGCTCCTTTA TCTGGCAATA TGATGCACAT TGCTATTGTG 
GGCATCAACA ACCAGATGAG TCTGCTTCAG GACAGTGGGG ATGAAGACCA AGCTCAGGAA 
AGAGTAAACA AGTTGGCCAA AATTCTTAAA GAGGAAGAAG TGAGTTCAAG CCTCTGTTCT 
GCCGGTGTTG GTGTAATCAG CTGTATAATT CAGCGAGATG AAGGACGAAC ACCCATGAGA 
CATTCTTTCC ATTGGTCGTT GGAGAAACAG TATTATGTAG AAGAGCCGTT GCTGCGTCAT 
CTTGAACCTC CTCTGTCCAT TTACCTTGAG TTGGATAAGC TGAAAGGATA CTCAAATATA 
CAATATACGC CTTCTCGAGA TCGTCAATGG CATCTGTATA CTGTTACAGA CAAGCCAGTG 
CCAATCAAGA GGATGTTCCT GAGATCTCTT GTTCGACAGG CTACAATGAA CGATGGATTT 
ATATTGCAGC AAGGGCAGGA TAAGCAGCTT AGCCAAACAC TGATCTCCAT GGCGTTTACG 
TCGAAATGTG TTCTGAGGTC TTTGATGGAT GCCATGGAGG AACTGGAACT GAATGCCCAT 
AATGCTGCAA TGAAACCAGA TCACGCACAT ATGTTTCTTT GCATATTGCG TGAGCAGCAG 
ATAGATGATC TTGTGCCTTT CCCCAGGAGA GTTGAAGTGA ATGCGGAGGA TGAAGAAACT 
ACAGTTGAAA TGATCTTAGA AGAAGCAGCA CGAGAGATAC ATAGATCTGT TGGAGTGAGA 
ATGCATAGGT TGGGCGTGTG CGAGTGGGAA GTGCGGCTGT GGTTGGTGTC CTCTGGACTG 
GCATGTGGTG CTTGGAGGGT TGTGGTTGCA AACGTGACAG GCCGTACATG CACTGTCCAC 
ATATACCGAG AAGTTGAAAC TCCTGGAAGA AACAGTTTAA TCTACCACTC AATAACCAAG 
AAGGGACCTT TGCATGAAAC ACCAATCAGT GATCAATATA AGCCCCTGGG ATATCTCGAC 
AGGCAACGTT TAGCAGCAAG GAGGAGTAAC ACTACTTATT GCTATGACTT CCCGTTGGCA 
TTTGGGACAG CCTTGGAACT GTTGTGGGCA TCACAACACC CAGGAGTTAA GAAACCATAT 
AAGGATACTC TGATCAATGT TAAAGAGCTT GTATTCTCAA AACCAGAAGG TTCTTCGGGT 
ACATCTCTAG ATCTGGTTGA AAGACCACCC GGTCTCAACG ACTTTGGAAT GGTTGCCTGG 
TGCCTAGATA TGTCGACCCC AGAGTTTCCT ATGGGGCGGA AACTTCTCGT GATTGCGAAT 
GATGTCACCT TCAAAGCTGG TTCTTTTGGT CCTAGAGAGG ACGCGTTTTT CCTTGCTGTT 
ACTGAACTCG CTTGTGCCAA GAAGCTTCCC TTGATTTACT TGGCAGCAAA TTCTGGTGCC 
CGACTTGGGG TTGCTGAAGA AGTCAAAGCC TGCTTCAAAG TTGGATGGTC GGATGAAATT 
TCCCCTGAGA ATGGTTTTCA GTATATATAC CTAAGCCCTG AAGACCACGA AAGGATTGGA 
TCATCTGTCA TTGCCCATGA AGTAAAGCTC TCTAGTGGGG AAACTAGGTG GGTGATTGAT 
ACGATCGTTG GCAAAGAAGA TGGTATTGGT GTAGAGAACT TAACAGGAAG TGGGGCCATA 
GCGGGTGCTT ACTCAAAGGC ATACAATGAA ACTTTTACTT TAACCTTTGT TAGTGGAAGA 
ACGGTTGGAA TTGGTGCTTA TCTTGCCCGC CTAGGTATGC GGTGCATACA GAGACTTGAT 
CAGCCGATCA TCTTGACTGG CTTCTCTACA CTCAACAAGT TACTTGGGCG TGAGGTCTAT 
AGCTCTCACA TGCAACTGGG TGGCCCGAAA ATCATGGGCA CAAATGGTGT TGTTCATCTT 
ACAGTCTCAG ATGATCTTGA AGGCGTATCA GCAATTCTCA ACTGGCTCAG CTACATTCCT 
GCTTACGTGG GTGGTCCTCT TCCTGTTCTT GCCCCTTTAG ATCCACCGGA GAGAATTGTG 
GAGTATGTCC CAGAGAACTC TTGCGACCCA CGAGCGGCTA TAGCTGGGGT CAAAGACAAT 
ACCGGTAAAT GGCTTGGAGG TATCTTTGAT AAAAATAGTT TCATTGAGAC TCTTGAAGGC 
TGGGCAAGGA CGGTAGTGAC TGGTAGAGCC AAGCTCGGGG GAATACCCGT TGGAGTTGTT 
GCAGTTGAGA CACAGACTGT CATGCAGATC ATCCCAGCCG ATCCTGGACA GCTTGACTCT 
CATGAAAGAG TGGTTCCGCA AGCAGGGCAA GTCTGGTTTC CTGATTCAGC GGCCAAGACT 
GCTCAAGCGC TTATGGATTT CAACCGGGAA GAGCTTCCAT TGTTTATCCT AGCGAACTGG 
AGAGGGTTTT CAGGTGGGCA GAGAGATCTT TTCGAAGGAA TACTTCAGGC AGGTTCAACT 
ATAGTAGAAA ATCTGAGAAC CTATCGTCAG CCAGTGTTTG TGTACATCCC AATGATGGGA 
GAGCTGCGCG GTGGAGCGTG GGTTGTTGTT GACAGCCAGA TAAATTCGGA TTATGTTGAA 
ATGTATGCTG ATGAAACAGC TCGTGGAAAT GTGCTTGAGC CAGAAGGGAC AATAGAGATA 
AAATTTAGAA CAAAAGAGCT ATTAGAGTGC ATGGGAAGGT TGGACCAGAA GCTAATCAGT 
CTGAAAGCAA AACTGCAAGA TGCCAAGCAA AGCGAGGCCT ATGCAAACAT CGAGCTTCTC 
CAGCAACAGA TTAAAGCCCG AGAGAAACAG CTTTTACCAG TTTATATCCA AATCGCCACC 
AAATTTGCAG AACTTCATGA CACTTCCATG AGAATGGCTG CAAAGGGAGT GATCAAAAGT 
GTTGTGGAAT GGAGCGGCTC GCGGTCCTTC TTCTACAAAA AGCTCAATAG GAGAATCGCT 
GAGAGCTCTC TTGTGAAAAA CGTAAGAGAA GCATCTGGAG ACAACTTAGC ATATAAATCT 
TCAATGCGTC TGATTCAGGA TTGGTTCTGC AACTCTGATA TTGCAAAGGG GAAAGAAGAA 
GCTTGGACAG ACGACCAAGT GTTCTTTACA TGGAAGGACA ATGTTAGTAA CTACGAGTTG 
AAGCTGAGCG AGTTGAGAGC GCAGAAACTA CTGAACCAAC TTGCAGAGAT TGGGAATTCC 
TCAGATTTGC AAGCTCTGCC ACAAGGACTT GCTAATCTTC TAAACAAGGT GGAGCCGTCG 
AAAAGAGAAG AGCTGGTGGC TGCTATTCGA AAGGTCTTGG GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACC1 ( NM_103313.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_174849.2 Arabidopsis thaliana acetyl-CoA carboxylase 1 (ACC1), mRNA.

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CloneID	OAb25573
Clone ID Related Accession (Same CDS sequence)	NM_001198214.2 , NM_103313.4
Accession Version	NM_103313.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	acetyl-CoA carboxylase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_103313.3.

ATGGCTGGCT CGGTTAACGG GAATCATAGT GCTGTAGGAC CTGGTATAAA TTATGAGACG 
GTGTCTCAAG TGGATGAGTT CTGTAAAGCA CTTAGAGGGA AAAGGCCGAT CCATAGTATT 
TTGATAGCTA ACAATGGAAT GGCGGCTGTG AAGTTTATAC GTAGTGTCAG AACATGGGCT 
TATGAAACAT TTGGTACGGA AAAAGCCATA TTGTTGGTGG GGATGGCAAC CCCTGAAGAC 
ATGCGGATCA ATGCGGAGCA TATCAGAATC GCTGATCAGT TTGTTGAGGT TCCCGGAGGA 
ACCAACAATA ACAATTATGC TAACGTTCAG CTGATTGTGG AGATGGCTGA AGTAACACGC 
GTGGATGCAG TTTGGCCTGG TTGGGGTCAT GCATCTGAAA ACCCCGAATT ACCTGATGCC 
CTAGATGCAA AAGGAATCAT ATTTCTTGGT CCTCCAGCAT CTTCAATGGC AGCACTGGGA 
GATAAGATTG GTTCTTCGTT GATTGCACAA GCTGCTGATG TACCCACTCT GCCATGGAGT 
GGTTCCCATG TTAAAATACC TCCTAATAGC AACTTGGTAA CCATCCCAGA GGAGATCTAC 
CGGCAAGCAT GTGTCTACAC AACTGAAGAA GCGATTGCTA GCTGTCAAGT TGTCGGTTAC 
CCAGCAATGA TCAAAGCATC GTGGGGTGGT GGTGGTAAAG GAATCAGGAA GGTTCATAAT 
GATGATGAGG TTAGGGCTCT ATTCAAGCAA GTTCAGGGTG AGGTCCCAGG CTCACCAATA 
TTCATAATGA AGGTTGCGTC ACAGAGTCGG CATCTAGAGG TCCAGCTGCT CTGTGACAAG 
CATGGAAATG TTTCAGCTCT GCATAGCCGT GATTGTAGCG TCCAGAGAAG ACATCAAAAG 
ATCATAGAGG AGGGTCCAAT TACTGTGGCT CCGCCAGAAA CTGTCAAGAA ACTTGAACAA 
GCAGCTAGAA GGTTGGCTAA GAGTGTTAAC TATGTTGGAG CTGCTACTGT TGAGTATCTC 
TACAGTATGG ACACTGGGGA GTACTACTTC TTAGAGCTTA ACCCTCGCTT ACAGGTTGAG 
CATCCTGTCA CTGAGTGGAT TGCCGAGATA AATCTTCCTG CTGCCCAAGT TGCTGTGGGG 
ATGGGAATTC CTCTCTGGCA AATCCCTGAG ATAAGACGGT TCTATGGAAT AGAACATGGT 
GGAGGTTATG ATTCTTGGCG AAAAACATCT GTTGTAGCCT TCCCTTTTGA TTTTGATAAA 
GCTCAATCTA TAAGGCCAAA AGGTCATTGT GTGGCTGTAC GTGTGACAAG TGAGGATCCT 
GATGACGGGT TCAAACCAAC CAGCGGTAGA GTTCAGGAGT TGAGTTTTAA GAGCAAGCCA 
AATGTGTGGG CGTACTTCTC TGTCAAGTCT GGTGGAGGCA TCCACGAGTT CTCGGATTCC 
CAGTTTGGAC ATGTTTTTGC ATTTGGGGAA TCCAGAGCCC TGGCGATAGC GAATATGGTT 
CTTGGGCTAA AAGAAATTCA GATCCGTGGA GAAATTAGGA CTAACGTTGA CTACACGATC 
GACCTTTTAC ATGCTTCTGA TTACCGTGAT AACAAAATTC ACACTGGTTG GTTGGATAGT 
AGGATTGCTA TGCGGGTCAG AGCTGAGAGG CCTCCATGGT ATCTCTCTGT TGTCGGCGGA 
GCTCTCTATA AAGCATCAGC GACCAGTGCT GCTGTGGTTT CAGATTACGT TGGTTATCTG 
GAGAAGGGGC AAATCCCTCC AAAGCATATA TCTCTTGTAC ATTCTCAAGT GTCTCTGAAT 
ATTGAAGGAA GTAAATATAC GATTGATGTA GTCCGGGGTG GATCAGGAAC CTACAGGCTA 
AGAATGAACA AGTCAGAAGT GGTAGCAGAA ATACACACTC TACGTGATGG AGGTCTGTTG 
ATGCAGTTGG ATGGCAAAAG CCATGTGATA TATGCAGAGG AAGAAGCTGC AGGAACTCGT 
CTTCTCATTG ATGGAAGAAC TTGTTTGCTA CAGAATGACC ACGATCCATC AAAGTTAATG 
GCTGAGACAC CGTGCAAGTT GATGAGGTAT TTGATTTCCG ACAACAGCAA TATTGACGCT 
GATACGCCTT ATGCCGAAGT TGAGGTCATG AAGATGTGCA TGCCACTTCT TTCACCTGCT 
TCAGGAGTTA TCCATTTTAA AATGTCTGAA GGACAAGCCA TGCAGGCTGG TGAACTTATA 
GCCAATCTTG ATCTTGATGA TCCTTCTGCT GTAAGAAAGG CCGAACCCTT CCATGGAAGT 
TTCCCAAGAT TAGGGCTTCC AACTGCAATA TCCGGTAGAG TTCATCAGAG ATGTGCCGCA 
ACATTAAATG CTGCACGCAT GATTCTTGCT GGCTATGAGC ATAAAGTAGA TGAGGTTGTT 
CAAGACTTAC TTAATTGCCT TGATAGCCCT GAACTCCCAT TTCTTCAGTG GCAAGAGTGC 
TTTGCAGTTC TGGCGACACG ACTACCTAAA AATCTCAGGA ACATGCTAGA ATCAAAGTAT 
AGGGAATTTG AGAGTATTTC CAGAAACTCT TTGACCACCG ATTTCCCTGC CAAACTTTTA 
AAAGGCATTC TTGAGGCACA TTTATCTTCT TGTGATGAGA AAGAGAGAGG TGCCCTTGAA 
AGGCTCATTG AACCATTGAT GAGCCTTGCA AAATCTTATG AAGGTGGTAG AGAAAGTCAT 
GCCCGTGTTA TTGTTCATTC TCTCTTTGAA GAATATCTAT CAGTAGAAGA ATTATTCAAT 
GATAACATGC TGGCTGATGT TATAGAACGC ATGCGTCAGC TATACAAGAA AGATCTGTTG 
AAAATTGTGG ATATAGTGCT CTCACACCAG GGCATAAAAA ACAAAAACAA ACTCGTTCTC 
CGGCTCATGG AGCAGCTTGT TTACCCTAAT CCTGCTGCTT ACAGAGATAA ACTTATTCGA 
TTCTCAACAC TTAACCATAC TAACTACTCT GAGTTGGCGC TCAAGGCGAG TCAATTACTT 
GAACAGACCA AACTAAGTGA GCTTCGTTCA AACATTGCTA GAAGCCTTTC AGAGTTAGAA 
ATGTTTACAG AGGACGGAGA AAATATGGAT ACTCCCAAGA GGAAAAGTGC CATTAATGAA 
AGAATAGAAG ATCTTGTAAG CGCATCTTTA GCTGTTGAAG ACGCTCTCGT GGGACTATTT 
GACCATAGCG ATCACACACT TCAAAGACGG GTTGTTGAGA CTTATATTCG CAGATTATAC 
CAGCCCTACG TCGTTAAAGA TAGCGTGAGG ATGCAGTGGC ACCGTTCTGG TCTTCTTGCT 
TCCTGGGAGT TCCTAGAGGA GCATATGGAA AGAAAAAACA TTGGCTTAGA CGATCCCGAC 
ACATCTGAAA AAGGATTGGT TGAGAAGCGT AGTAAGAGAA AATGGGGGGC TATGGTTATA 
ATCAAATCTT TGCAGTTTCT TCCAAGTATA ATAAGTGCAG CATTGAGAGA AACAAAGCAC 
AACGACTATG AAACTGCCGG AGCTCCTTTA TCTGGCAATA TGATGCACAT TGCTATTGTG 
GGCATCAACA ACCAGATGAG TCTGCTTCAG GACAGTGGGG ATGAAGACCA AGCTCAGGAA 
AGAGTAAACA AGTTGGCCAA AATTCTTAAA GAGGAAGAAG TGAGTTCAAG CCTCTGTTCT 
GCCGGTGTTG GTGTAATCAG CTGTATAATT CAGCGAGATG AAGGACGAAC ACCCATGAGA 
CATTCTTTCC ATTGGTCGTT GGAGAAACAG TATTATGTAG AAGAGCCGTT GCTGCGTCAT 
CTTGAACCTC CTCTGTCCAT TTACCTTGAG TTGGATAAGC TGAAAGGATA CTCAAATATA 
CAATATACGC CTTCTCGAGA TCGTCAATGG CATCTGTATA CTGTTACAGA CAAGCCAGTG 
CCAATCAAGA GGATGTTCCT GAGATCTCTT GTTCGACAGG CTACAATGAA CGATGGATTT 
ATATTGCAGC AAGGGCAGGA TAAGCAGCTT AGCCAAACAC TGATCTCCAT GGCGTTTACG 
TCGAAATGTG TTCTGAGGTC TTTGATGGAT GCCATGGAGG AACTGGAACT GAATGCCCAT 
AATGCTGCAA TGAAACCAGA TCACGCACAT ATGTTTCTTT GCATATTGCG TGAGCAGCAG 
ATAGATGATC TTGTGCCTTT CCCCAGGAGA GTTGAAGTGA ATGCGGAGGA TGAAGAAACT 
ACAGTTGAAA TGATCTTAGA AGAAGCAGCA CGAGAGATAC ATAGATCTGT TGGAGTGAGA 
ATGCATAGGT TGGGCGTGTG CGAGTGGGAA GTGCGGCTGT GGTTGGTGTC CTCTGGACTG 
GCATGTGGTG CTTGGAGGGT TGTGGTTGCA AACGTGACAG GCCGTACATG CACTGTCCAC 
ATATACCGAG AAGTTGAAAC TCCTGGAAGA AACAGTTTAA TCTACCACTC AATAACCAAG 
AAGGGACCTT TGCATGAAAC ACCAATCAGT GATCAATATA AGCCCCTGGG ATATCTCGAC 
AGGCAACGTT TAGCAGCAAG GAGGAGTAAC ACTACTTATT GCTATGACTT CCCGTTGGCA 
TTTGGGACAG CCTTGGAACT GTTGTGGGCA TCACAACACC CAGGAGTTAA GAAACCATAT 
AAGGATACTC TGATCAATGT TAAAGAGCTT GTATTCTCAA AACCAGAAGG TTCTTCGGGT 
ACATCTCTAG ATCTGGTTGA AAGACCACCC GGTCTCAACG ACTTTGGAAT GGTTGCCTGG 
TGCCTAGATA TGTCGACCCC AGAGTTTCCT ATGGGGCGGA AACTTCTCGT GATTGCGAAT 
GATGTCACCT TCAAAGCTGG TTCTTTTGGT CCTAGAGAGG ACGCGTTTTT CCTTGCTGTT 
ACTGAACTCG CTTGTGCCAA GAAGCTTCCC TTGATTTACT TGGCAGCAAA TTCTGGTGCC 
CGACTTGGGG TTGCTGAAGA AGTCAAAGCC TGCTTCAAAG TTGGATGGTC GGATGAAATT 
TCCCCTGAGA ATGGTTTTCA GTATATATAC CTAAGCCCTG AAGACCACGA AAGGATTGGA 
TCATCTGTCA TTGCCCATGA AGTAAAGCTC TCTAGTGGGG AAACTAGGTG GGTGATTGAT 
ACGATCGTTG GCAAAGAAGA TGGTATTGGT GTAGAGAACT TAACAGGAAG TGGGGCCATA 
GCGGGTGCTT ACTCAAAGGC ATACAATGAA ACTTTTACTT TAACCTTTGT TAGTGGAAGA 
ACGGTTGGAA TTGGTGCTTA TCTTGCCCGC CTAGGTATGC GGTGCATACA GAGACTTGAT 
CAGCCGATCA TCTTGACTGG CTTCTCTACA CTCAACAAGT TACTTGGGCG TGAGGTCTAT 
AGCTCTCACA TGCAACTGGG TGGCCCGAAA ATCATGGGCA CAAATGGTGT TGTTCATCTT 
ACAGTCTCAG ATGATCTTGA AGGCGTATCA GCAATTCTCA ACTGGCTCAG CTACATTCCT 
GCTTACGTGG GTGGTCCTCT TCCTGTTCTT GCCCCTTTAG ATCCACCGGA GAGAATTGTG 
GAGTATGTCC CAGAGAACTC TTGCGACCCA CGAGCGGCTA TAGCTGGGGT CAAAGACAAT 
ACCGGTAAAT GGCTTGGAGG TATCTTTGAT AAAAATAGTT TCATTGAGAC TCTTGAAGGC 
TGGGCAAGGA CGGTAGTGAC TGGTAGAGCC AAGCTCGGGG GAATACCCGT TGGAGTTGTT 
GCAGTTGAGA CACAGACTGT CATGCAGATC ATCCCAGCCG ATCCTGGACA GCTTGACTCT 
CATGAAAGAG TGGTTCCGCA AGCAGGGCAA GTCTGGTTTC CTGATTCAGC GGCCAAGACT 
GCTCAAGCGC TTATGGATTT CAACCGGGAA GAGCTTCCAT TGTTTATCCT AGCGAACTGG 
AGAGGGTTTT CAGGTGGGCA GAGAGATCTT TTCGAAGGAA TACTTCAGGC AGGTTCAACT 
ATAGTAGAAA ATCTGAGAAC CTATCGTCAG CCAGTGTTTG TGTACATCCC AATGATGGGA 
GAGCTGCGCG GTGGAGCGTG GGTTGTTGTT GACAGCCAGA TAAATTCGGA TTATGTTGAA 
ATGTATGCTG ATGAAACAGC TCGTGGAAAT GTGCTTGAGC CAGAAGGGAC AATAGAGATA 
AAATTTAGAA CAAAAGAGCT ATTAGAGTGC ATGGGAAGGT TGGACCAGAA GCTAATCAGT 
CTGAAAGCAA AACTGCAAGA TGCCAAGCAA AGCGAGGCCT ATGCAAACAT CGAGCTTCTC 
CAGCAACAGA TTAAAGCCCG AGAGAAACAG CTTTTACCAG TTTATATCCA AATCGCCACC 
AAATTTGCAG AACTTCATGA CACTTCCATG AGAATGGCTG CAAAGGGAGT GATCAAAAGT 
GTTGTGGAAT GGAGCGGCTC GCGGTCCTTC TTCTACAAAA AGCTCAATAG GAGAATCGCT 
GAGAGCTCTC TTGTGAAAAA CGTAAGAGAA GCATCTGGAG ACAACTTAGC ATATAAATCT 
TCAATGCGTC TGATTCAGGA TTGGTTCTGC AACTCTGATA TTGCAAAGGG GAAAGAAGAA 
GCTTGGACAG ACGACCAAGT GTTCTTTACA TGGAAGGACA ATGTTAGTAA CTACGAGTTG 
AAGCTGAGCG AGTTGAGAGC GCAGAAACTA CTGAACCAAC TTGCAGAGAT TGGGAATTCC 
TCAGATTTGC AAGCTCTGCC ACAAGGACTT GCTAATCTTC TAAACAAGGT GGAGCCGTCG 
AAAAGAGAAG AGCTGGTGGC TGCTATTCGA AAGGTCTTGG GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_174849.2
CDS	393..7157
Translation	MAGSVNGNHSAVGPGINYETVSQVDEFCKALRGKRPIHSILIANNGMAAVKFIRSVRTWAYETFGTEKAILLVGMATPEDMRINAEHIRIADQFVEVPGGTNNNNYANVQLIVEMAEVTRVDAVWPGWGHASENPELPDALDAKGIIFLGPPASSMAALGDKIGSSLIAQAADVPTLPWSGSHVKIPPNSNLVTIPEEIYRQACVYTTEEAIASCQVVGYPAMIKASWGGGGKGIRKVHNDDEVRALFKQVQGEVPGSPIFIMKVASQSRHLEVQLLCDKHGNVSALHSRDCSVQRRHQKIIEEGPITVAPPETVKKLEQAARRLAKSVNYVGAATVEYLYSMDTGEYYFLELNPRLQVEHPVTEWIAEINLPAAQVAVGMGIPLWQIPEIRRFYGIEHGGGYDSWRKTSVVAFPFDFDKAQSIRPKGHCVAVRVTSEDPDDGFKPTSGRVQELSFKSKPNVWAYFSVKSGGGIHEFSDSQFGHVFAFGESRALAIANMVLGLKEIQIRGEIRTNVDYTIDLLHASDYRDNKIHTGWLDSRIAMRVRAERPPWYLSVVGGALYKASATSAAVVSDYVGYLEKGQIPPKHISLVHSQVSLNIEGSKYTIDVVRGGSGTYRLRMNKSEVVAEIHTLRDGGLLMQLDGKSHVIYAEEEAAGTRLLIDGRTCLLQNDHDPSKLMAETPCKLMRYLISDNSNIDADTPYAEVEVMKMCMPLLSPASGVIHFKMSEGQAMQAGELIANLDLDDPSAVRKAEPFHGSFPRLGLPTAISGRVHQRCAATLNAARMILAGYEHKVDEVVQDLLNCLDSPELPFLQWQECFAVLATRLPKNLRNMLESKYREFESISRNSLTTDFPAKLLKGILEAHLSSCDEKERGALERLIEPLMSLAKSYEGGRESHARVIVHSLFEEYLSVEELFNDNMLADVIERMRQLYKKDLLKIVDIVLSHQGIKNKNKLVLRLMEQLVYPNPAAYRDKLIRFSTLNHTNYSELALKASQLLEQTKLSELRSNIARSLSELEMFTEDGENMDTPKRKSAINERIEDLVSASLAVEDALVGLFDHSDHTLQRRVVETYIRRLYQPYVVKDSVRMQWHRSGLLASWEFLEEHMERKNIGLDDPDTSEKGLVEKRSKRKWGAMVIIKSLQFLPSIISAALRETKHNDYETAGAPLSGNMMHIAIVGINNQMSLLQDSGDEDQAQERVNKLAKILKEEEVSSSLCSAGVGVISCIIQRDEGRTPMRHSFHWSLEKQYYVEEPLLRHLEPPLSIYLELDKLKGYSNIQYTPSRDRQWHLYTVTDKPVPIKRMFLRSLVRQATMNDGFILQQGQDKQLSQTLISMAFTSKCVLRSLMDAMEELELNAHNAAMKPDHAHMFLCILREQQIDDLVPFPRRVEVNAEDEETTVEMILEEAAREIHRSVGVRMHRLGVCEWEVRLWLVSSGLACGAWRVVVANVTGRTCTVHIYREVETPGRNSLIYHSITKKGPLHETPISDQYKPLGYLDRQRLAARRSNTTYCYDFPLAFGTALELLWASQHPGVKKPYKDTLINVKELVFSKPEGSSGTSLDLVERPPGLNDFGMVAWCLDMSTPEFPMGRKLLVIANDVTFKAGSFGPREDAFFLAVTELACAKKLPLIYLAANSGARLGVAEEVKACFKVGWSDEISPENGFQYIYLSPEDHERIGSSVIAHEVKLSSGETRWVIDTIVGKEDGIGVENLTGSGAIAGAYSKAYNETFTLTFVSGRTVGIGAYLARLGMRCIQRLDQPIILTGFSTLNKLLGREVYSSHMQLGGPKIMGTNGVVHLTVSDDLEGVSAILNWLSYIPAYVGGPLPVLAPLDPPERIVEYVPENSCDPRAAIAGVKDNTGKWLGGIFDKNSFIETLEGWARTVVTGRAKLGGIPVGVVAVETQTVMQIIPADPGQLDSHERVVPQAGQVWFPDSAAKTAQALMDFNREELPLFILANWRGFSGGQRDLFEGILQAGSTIVENLRTYRQPVFVYIPMMGELRGGAWVVVDSQINSDYVEMYADETARGNVLEPEGTIEIKFRTKELLECMGRLDQKLISLKAKLQDAKQSEAYANIELLQQQIKAREKQLLPVYIQIATKFAELHDTSMRMAAKGVIKSVVEWSGSRSFFYKKLNRRIAESSLVKNVREASGDNLAYKSSMRLIQDWFCNSDIAKGKEEAWTDDQVFFTWKDNVSNYELKLSELRAQKLLNQLAEIGNSSDLQALPQGLANLLNKVEPSKREELVAAIRKVLG

Target ORF information:

RefSeq Version	NM_103313.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana acetyl-CoA carboxylase 1 (ACC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_103313.4

ORF Insert Sequence:

ATGGCTGGCT CGGTTAACGG GAATCATAGT GCTGTAGGAC CTGGTATAAA TTATGAGACG 
GTGTCTCAAG TGGATGAGTT CTGTAAAGCA CTTAGAGGGA AAAGGCCGAT CCATAGTATT 
TTGATAGCTA ACAATGGAAT GGCGGCTGTG AAGTTTATAC GTAGTGTCAG AACATGGGCT 
TATGAAACAT TTGGTACGGA AAAAGCCATA TTGTTGGTGG GGATGGCAAC CCCTGAAGAC 
ATGCGGATCA ATGCGGAGCA TATCAGAATC GCTGATCAGT TTGTTGAGGT TCCCGGAGGA 
ACCAACAATA ACAATTATGC TAACGTTCAG CTGATTGTGG AGATGGCTGA AGTAACACGC 
GTGGATGCAG TTTGGCCTGG TTGGGGTCAT GCATCTGAAA ACCCCGAATT ACCTGATGCC 
CTAGATGCAA AAGGAATCAT ATTTCTTGGT CCTCCAGCAT CTTCAATGGC AGCACTGGGA 
GATAAGATTG GTTCTTCGTT GATTGCACAA GCTGCTGATG TACCCACTCT GCCATGGAGT 
GGTTCCCATG TTAAAATACC TCCTAATAGC AACTTGGTAA CCATCCCAGA GGAGATCTAC 
CGGCAAGCAT GTGTCTACAC AACTGAAGAA GCGATTGCTA GCTGTCAAGT TGTCGGTTAC 
CCAGCAATGA TCAAAGCATC GTGGGGTGGT GGTGGTAAAG GAATCAGGAA GGTTCATAAT 
GATGATGAGG TTAGGGCTCT ATTCAAGCAA GTTCAGGGTG AGGTCCCAGG CTCACCAATA 
TTCATAATGA AGGTTGCGTC ACAGAGTCGG CATCTAGAGG TCCAGCTGCT CTGTGACAAG 
CATGGAAATG TTTCAGCTCT GCATAGCCGT GATTGTAGCG TCCAGAGAAG ACATCAAAAG 
ATCATAGAGG AGGGTCCAAT TACTGTGGCT CCGCCAGAAA CTGTCAAGAA ACTTGAACAA 
GCAGCTAGAA GGTTGGCTAA GAGTGTTAAC TATGTTGGAG CTGCTACTGT TGAGTATCTC 
TACAGTATGG ACACTGGGGA GTACTACTTC TTAGAGCTTA ACCCTCGCTT ACAGGTTGAG 
CATCCTGTCA CTGAGTGGAT TGCCGAGATA AATCTTCCTG CTGCCCAAGT TGCTGTGGGG 
ATGGGAATTC CTCTCTGGCA AATCCCTGAG ATAAGACGGT TCTATGGAAT AGAACATGGT 
GGAGGTTATG ATTCTTGGCG AAAAACATCT GTTGTAGCCT TCCCTTTTGA TTTTGATAAA 
GCTCAATCTA TAAGGCCAAA AGGTCATTGT GTGGCTGTAC GTGTGACAAG TGAGGATCCT 
GATGACGGGT TCAAACCAAC CAGCGGTAGA GTTCAGGAGT TGAGTTTTAA GAGCAAGCCA 
AATGTGTGGG CGTACTTCTC TGTCAAGTCT GGTGGAGGCA TCCACGAGTT CTCGGATTCC 
CAGTTTGGAC ATGTTTTTGC ATTTGGGGAA TCCAGAGCCC TGGCGATAGC GAATATGGTT 
CTTGGGCTAA AAGAAATTCA GATCCGTGGA GAAATTAGGA CTAACGTTGA CTACACGATC 
GACCTTTTAC ATGCTTCTGA TTACCGTGAT AACAAAATTC ACACTGGTTG GTTGGATAGT 
AGGATTGCTA TGCGGGTCAG AGCTGAGAGG CCTCCATGGT ATCTCTCTGT TGTCGGCGGA 
GCTCTCTATA AAGCATCAGC GACCAGTGCT GCTGTGGTTT CAGATTACGT TGGTTATCTG 
GAGAAGGGGC AAATCCCTCC AAAGCATATA TCTCTTGTAC ATTCTCAAGT GTCTCTGAAT 
ATTGAAGGAA GTAAATATAC GATTGATGTA GTCCGGGGTG GATCAGGAAC CTACAGGCTA 
AGAATGAACA AGTCAGAAGT GGTAGCAGAA ATACACACTC TACGTGATGG AGGTCTGTTG 
ATGCAGTTGG ATGGCAAAAG CCATGTGATA TATGCAGAGG AAGAAGCTGC AGGAACTCGT 
CTTCTCATTG ATGGAAGAAC TTGTTTGCTA CAGAATGACC ACGATCCATC AAAGTTAATG 
GCTGAGACAC CGTGCAAGTT GATGAGGTAT TTGATTTCCG ACAACAGCAA TATTGACGCT 
GATACGCCTT ATGCCGAAGT TGAGGTCATG AAGATGTGCA TGCCACTTCT TTCACCTGCT 
TCAGGAGTTA TCCATTTTAA AATGTCTGAA GGACAAGCCA TGCAGGCTGG TGAACTTATA 
GCCAATCTTG ATCTTGATGA TCCTTCTGCT GTAAGAAAGG CCGAACCCTT CCATGGAAGT 
TTCCCAAGAT TAGGGCTTCC AACTGCAATA TCCGGTAGAG TTCATCAGAG ATGTGCCGCA 
ACATTAAATG CTGCACGCAT GATTCTTGCT GGCTATGAGC ATAAAGTAGA TGAGGTTGTT 
CAAGACTTAC TTAATTGCCT TGATAGCCCT GAACTCCCAT TTCTTCAGTG GCAAGAGTGC 
TTTGCAGTTC TGGCGACACG ACTACCTAAA AATCTCAGGA ACATGCTAGA ATCAAAGTAT 
AGGGAATTTG AGAGTATTTC CAGAAACTCT TTGACCACCG ATTTCCCTGC CAAACTTTTA 
AAAGGCATTC TTGAGGCACA TTTATCTTCT TGTGATGAGA AAGAGAGAGG TGCCCTTGAA 
AGGCTCATTG AACCATTGAT GAGCCTTGCA AAATCTTATG AAGGTGGTAG AGAAAGTCAT 
GCCCGTGTTA TTGTTCATTC TCTCTTTGAA GAATATCTAT CAGTAGAAGA ATTATTCAAT 
GATAACATGC TGGCTGATGT TATAGAACGC ATGCGTCAGC TATACAAGAA AGATCTGTTG 
AAAATTGTGG ATATAGTGCT CTCACACCAG GGCATAAAAA ACAAAAACAA ACTCGTTCTC 
CGGCTCATGG AGCAGCTTGT TTACCCTAAT CCTGCTGCTT ACAGAGATAA ACTTATTCGA 
TTCTCAACAC TTAACCATAC TAACTACTCT GAGTTGGCGC TCAAGGCGAG TCAATTACTT 
GAACAGACCA AACTAAGTGA GCTTCGTTCA AACATTGCTA GAAGCCTTTC AGAGTTAGAA 
ATGTTTACAG AGGACGGAGA AAATATGGAT ACTCCCAAGA GGAAAAGTGC CATTAATGAA 
AGAATAGAAG ATCTTGTAAG CGCATCTTTA GCTGTTGAAG ACGCTCTCGT GGGACTATTT 
GACCATAGCG ATCACACACT TCAAAGACGG GTTGTTGAGA CTTATATTCG CAGATTATAC 
CAGCCCTACG TCGTTAAAGA TAGCGTGAGG ATGCAGTGGC ACCGTTCTGG TCTTCTTGCT 
TCCTGGGAGT TCCTAGAGGA GCATATGGAA AGAAAAAACA TTGGCTTAGA CGATCCCGAC 
ACATCTGAAA AAGGATTGGT TGAGAAGCGT AGTAAGAGAA AATGGGGGGC TATGGTTATA 
ATCAAATCTT TGCAGTTTCT TCCAAGTATA ATAAGTGCAG CATTGAGAGA AACAAAGCAC 
AACGACTATG AAACTGCCGG AGCTCCTTTA TCTGGCAATA TGATGCACAT TGCTATTGTG 
GGCATCAACA ACCAGATGAG TCTGCTTCAG GACAGTGGGG ATGAAGACCA AGCTCAGGAA 
AGAGTAAACA AGTTGGCCAA AATTCTTAAA GAGGAAGAAG TGAGTTCAAG CCTCTGTTCT 
GCCGGTGTTG GTGTAATCAG CTGTATAATT CAGCGAGATG AAGGACGAAC ACCCATGAGA 
CATTCTTTCC ATTGGTCGTT GGAGAAACAG TATTATGTAG AAGAGCCGTT GCTGCGTCAT 
CTTGAACCTC CTCTGTCCAT TTACCTTGAG TTGGATAAGC TGAAAGGATA CTCAAATATA 
CAATATACGC CTTCTCGAGA TCGTCAATGG CATCTGTATA CTGTTACAGA CAAGCCAGTG 
CCAATCAAGA GGATGTTCCT GAGATCTCTT GTTCGACAGG CTACAATGAA CGATGGATTT 
ATATTGCAGC AAGGGCAGGA TAAGCAGCTT AGCCAAACAC TGATCTCCAT GGCGTTTACG 
TCGAAATGTG TTCTGAGGTC TTTGATGGAT GCCATGGAGG AACTGGAACT GAATGCCCAT 
AATGCTGCAA TGAAACCAGA TCACGCACAT ATGTTTCTTT GCATATTGCG TGAGCAGCAG 
ATAGATGATC TTGTGCCTTT CCCCAGGAGA GTTGAAGTGA ATGCGGAGGA TGAAGAAACT 
ACAGTTGAAA TGATCTTAGA AGAAGCAGCA CGAGAGATAC ATAGATCTGT TGGAGTGAGA 
ATGCATAGGT TGGGCGTGTG CGAGTGGGAA GTGCGGCTGT GGTTGGTGTC CTCTGGACTG 
GCATGTGGTG CTTGGAGGGT TGTGGTTGCA AACGTGACAG GCCGTACATG CACTGTCCAC 
ATATACCGAG AAGTTGAAAC TCCTGGAAGA AACAGTTTAA TCTACCACTC AATAACCAAG 
AAGGGACCTT TGCATGAAAC ACCAATCAGT GATCAATATA AGCCCCTGGG ATATCTCGAC 
AGGCAACGTT TAGCAGCAAG GAGGAGTAAC ACTACTTATT GCTATGACTT CCCGTTGGCA 
TTTGGGACAG CCTTGGAACT GTTGTGGGCA TCACAACACC CAGGAGTTAA GAAACCATAT 
AAGGATACTC TGATCAATGT TAAAGAGCTT GTATTCTCAA AACCAGAAGG TTCTTCGGGT 
ACATCTCTAG ATCTGGTTGA AAGACCACCC GGTCTCAACG ACTTTGGAAT GGTTGCCTGG 
TGCCTAGATA TGTCGACCCC AGAGTTTCCT ATGGGGCGGA AACTTCTCGT GATTGCGAAT 
GATGTCACCT TCAAAGCTGG TTCTTTTGGT CCTAGAGAGG ACGCGTTTTT CCTTGCTGTT 
ACTGAACTCG CTTGTGCCAA GAAGCTTCCC TTGATTTACT TGGCAGCAAA TTCTGGTGCC 
CGACTTGGGG TTGCTGAAGA AGTCAAAGCC TGCTTCAAAG TTGGATGGTC GGATGAAATT 
TCCCCTGAGA ATGGTTTTCA GTATATATAC CTAAGCCCTG AAGACCACGA AAGGATTGGA 
TCATCTGTCA TTGCCCATGA AGTAAAGCTC TCTAGTGGGG AAACTAGGTG GGTGATTGAT 
ACGATCGTTG GCAAAGAAGA TGGTATTGGT GTAGAGAACT TAACAGGAAG TGGGGCCATA 
GCGGGTGCTT ACTCAAAGGC ATACAATGAA ACTTTTACTT TAACCTTTGT TAGTGGAAGA 
ACGGTTGGAA TTGGTGCTTA TCTTGCCCGC CTAGGTATGC GGTGCATACA GAGACTTGAT 
CAGCCGATCA TCTTGACTGG CTTCTCTACA CTCAACAAGT TACTTGGGCG TGAGGTCTAT 
AGCTCTCACA TGCAACTGGG TGGCCCGAAA ATCATGGGCA CAAATGGTGT TGTTCATCTT 
ACAGTCTCAG ATGATCTTGA AGGCGTATCA GCAATTCTCA ACTGGCTCAG CTACATTCCT 
GCTTACGTGG GTGGTCCTCT TCCTGTTCTT GCCCCTTTAG ATCCACCGGA GAGAATTGTG 
GAGTATGTCC CAGAGAACTC TTGCGACCCA CGAGCGGCTA TAGCTGGGGT CAAAGACAAT 
ACCGGTAAAT GGCTTGGAGG TATCTTTGAT AAAAATAGTT TCATTGAGAC TCTTGAAGGC 
TGGGCAAGGA CGGTAGTGAC TGGTAGAGCC AAGCTCGGGG GAATACCCGT TGGAGTTGTT 
GCAGTTGAGA CACAGACTGT CATGCAGATC ATCCCAGCCG ATCCTGGACA GCTTGACTCT 
CATGAAAGAG TGGTTCCGCA AGCAGGGCAA GTCTGGTTTC CTGATTCAGC GGCCAAGACT 
GCTCAAGCGC TTATGGATTT CAACCGGGAA GAGCTTCCAT TGTTTATCCT AGCGAACTGG 
AGAGGGTTTT CAGGTGGGCA GAGAGATCTT TTCGAAGGAA TACTTCAGGC AGGTTCAACT 
ATAGTAGAAA ATCTGAGAAC CTATCGTCAG CCAGTGTTTG TGTACATCCC AATGATGGGA 
GAGCTGCGCG GTGGAGCGTG GGTTGTTGTT GACAGCCAGA TAAATTCGGA TTATGTTGAA 
ATGTATGCTG ATGAAACAGC TCGTGGAAAT GTGCTTGAGC CAGAAGGGAC AATAGAGATA 
AAATTTAGAA CAAAAGAGCT ATTAGAGTGC ATGGGAAGGT TGGACCAGAA GCTAATCAGT 
CTGAAAGCAA AACTGCAAGA TGCCAAGCAA AGCGAGGCCT ATGCAAACAT CGAGCTTCTC 
CAGCAACAGA TTAAAGCCCG AGAGAAACAG CTTTTACCAG TTTATATCCA AATCGCCACC 
AAATTTGCAG AACTTCATGA CACTTCCATG AGAATGGCTG CAAAGGGAGT GATCAAAAGT 
GTTGTGGAAT GGAGCGGCTC GCGGTCCTTC TTCTACAAAA AGCTCAATAG GAGAATCGCT 
GAGAGCTCTC TTGTGAAAAA CGTAAGAGAA GCATCTGGAG ACAACTTAGC ATATAAATCT 
TCAATGCGTC TGATTCAGGA TTGGTTCTGC AACTCTGATA TTGCAAAGGG GAAAGAAGAA 
GCTTGGACAG ACGACCAAGT GTTCTTTACA TGGAAGGACA ATGTTAGTAA CTACGAGTTG 
AAGCTGAGCG AGTTGAGAGC GCAGAAACTA CTGAACCAAC TTGCAGAGAT TGGGAATTCC 
TCAGATTTGC AAGCTCTGCC ACAAGGACTT GCTAATCTTC TAAACAAGGT GGAGCCGTCG 
AAAAGAGAAG AGCTGGTGGC TGCTATTCGA AAGGTCTTGG GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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ACC1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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