Map4k4 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Map4k4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map4k4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa62690 XM_008767000.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ORa62691 XM_008767001.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ORa62692 XM_008767002.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ORa62693 XM_008767003.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ORa62694 XM_008767004.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ORa62695 XM_008767005.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ORa62696 XM_008767006.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ORa62697 XM_017596340.1
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ORa62698 XM_008767007.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa62699 XM_008767008.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa62700 XM_008767009.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa62701 XM_008767010.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa62702 XM_017596341.1
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa62703 XM_008767011.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa62704 XM_008767012.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa62705 XM_017596342.1
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa62706 XM_017596343.1
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa62707 XM_008767013.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa62708 XM_017596344.1
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa62709 XM_008767014.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa62710 XM_017596345.1
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa62711 XM_008767015.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa62712 XM_008767016.2
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
ORa62713 XM_017596346.1
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
ORa42317 NM_001106904.1
Latest version!
Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa62690
    Clone ID Related Accession (Same CDS sequence) XM_008767000.2
    Accession Version XM_008767000.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767000.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT
    TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG
    CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA
    TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC
    AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGG AGAAGTGGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG
    ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG
    GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT
    CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA
    GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGAGTACA
    GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC TTCCTCCCCA
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT
    AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGGTT ACTACAGATC TCTCCATCTA GTGGAACAAC AGTTACTTCC
    GTTGTGGGAT TTTCCTGTGA TGGGCTGAGA CCAGAAGCCA TAAGGCAAGA TCCTACTCGG
    AAAGGCTCAG TGGTCAATGT GAACCCCACG AACACTAGGC CACAGAGTGA TACCCCCGAG
    ATTCGTAAAT ACAAGAAGAG ATTTAACTCA GAGATCCTGT GTGCTGCCTT ATGGGGAGTG
    AATTTGCTAG TTGGCACAGA GAGTGGCCTG ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA
    GTGTACCCTC TGATCAGCCG GAGACGGTTC CAGCAAATGG ATGTACTTGA GGGCCTGAAT
    GTCCTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGCG TCTACTATTT ATCCTGGTTA
    AGAAATAAAA TACTTCATAA TGATCCGGAA GTTGAGAAGA AGCAGGGGTG GACCACCGTG
    GGCGACCTGG AAGGGTGCGT GCACTATAAA GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG
    GTAATCGCTT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CACCGAAGCC ATATCACAAA
    TTTATGGCCT TTAAGTCATT TGGAGAACTG GTACATAAGC CATTACTGGT GGATCTCACT
    GTCGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT
    GATGTGGATT CCGGCTCAGT CTATGACATT TATCTACCAA CACACGTAAG AAAGAACCCA
    CACTCTATGA TTCAGTGTAC CATCAAACCC CATGCAATCA TTATCCTCCC CAACACGGAT
    GGAATGGAGC TTCTGGTGTG CTACGAAGAT GAAGGGGTTT ATGTGAACAC GTACGGAAGA
    ATCACCAAGG ACGTGGTTCT GCAGTGGGGA GAGATGCCAA CATCTGTAGC ATACATCCGG
    TCGAATCAGA CAATGGGCTG GGGAGAGAAG GCAATAGAGA TACGCTCTGT GGAAACTGGT
    CATTTGGACG GTGTGTTTAT GCACAAAAGG GCTCAGAGAC TAAAGTTCCT GTGTGAACGT
    AATGACAAGG TGTTCTTTGC TTCCGTCCGC TCTGGCGGCA GCAGCCAGGT CTACTTCATG
    ACCTTAGGCA GGACTTCCCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765222.2
    CDS211..4383
    Translation

    Target ORF information:

    RefSeq Version XM_008767000.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767000.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT
    TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG
    CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA
    TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC
    AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGG AGAAGTGGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG
    ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG
    GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT
    CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA
    GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGAGTACA
    GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC TTCCTCCCCA
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT
    AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGGTT ACTACAGATC TCTCCATCTA GTGGAACAAC AGTTACTTCC
    GTTGTGGGAT TTTCCTGTGA TGGGCTGAGA CCAGAAGCCA TAAGGCAAGA TCCTACTCGG
    AAAGGCTCAG TGGTCAATGT GAACCCCACG AACACTAGGC CACAGAGTGA TACCCCCGAG
    ATTCGTAAAT ACAAGAAGAG ATTTAACTCA GAGATCCTGT GTGCTGCCTT ATGGGGAGTG
    AATTTGCTAG TTGGCACAGA GAGTGGCCTG ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA
    GTGTACCCTC TGATCAGCCG GAGACGGTTC CAGCAAATGG ATGTACTTGA GGGCCTGAAT
    GTCCTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGCG TCTACTATTT ATCCTGGTTA
    AGAAATAAAA TACTTCATAA TGATCCGGAA GTTGAGAAGA AGCAGGGGTG GACCACCGTG
    GGCGACCTGG AAGGGTGCGT GCACTATAAA GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG
    GTAATCGCTT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CACCGAAGCC ATATCACAAA
    TTTATGGCCT TTAAGTCATT TGGAGAACTG GTACATAAGC CATTACTGGT GGATCTCACT
    GTCGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT
    GATGTGGATT CCGGCTCAGT CTATGACATT TATCTACCAA CACACGTAAG AAAGAACCCA
    CACTCTATGA TTCAGTGTAC CATCAAACCC CATGCAATCA TTATCCTCCC CAACACGGAT
    GGAATGGAGC TTCTGGTGTG CTACGAAGAT GAAGGGGTTT ATGTGAACAC GTACGGAAGA
    ATCACCAAGG ACGTGGTTCT GCAGTGGGGA GAGATGCCAA CATCTGTAGC ATACATCCGG
    TCGAATCAGA CAATGGGCTG GGGAGAGAAG GCAATAGAGA TACGCTCTGT GGAAACTGGT
    CATTTGGACG GTGTGTTTAT GCACAAAAGG GCTCAGAGAC TAAAGTTCCT GTGTGAACGT
    AATGACAAGG TGTTCTTTGC TTCCGTCCGC TCTGGCGGCA GCAGCCAGGT CTACTTCATG
    ACCTTAGGCA GGACTTCCCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62691
    Clone ID Related Accession (Same CDS sequence) XM_008767001.2
    Accession Version XM_008767001.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT
    TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG
    CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGTA TTGAGCCCCG GCTTCTATGG
    GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC
    TCAGGATCCC AGCCTGGCTC CCATCCTGGG TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA
    GTGAGATCAT CATCCAAGTC TGAAGGTTCT CCATCTCAGC GCCTTGAAAA TGCAGCCAAA
    AAACCTGAAG ATAAGAAAGA AGTATTTCGA CCTCTCAAAC CTGCTGGAGA AGTGGACTTG
    ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTGC GGCCACCTCA CAAAGTGACA
    GACTATTCCT CCTCCAGTGA GGAGTCTGGG ACCACGGATG AGGAGGAGGA GGATGTGGAA
    CAGGAGGGGG CTGATGATTC CACCTCGGGG CCAGAGGACA CCAGAGCAGC GTCGTCTCTG
    AATCTGAGCA ACGGTGAGAC AGAATCTGTG AAAACAATGA TTGTTCATGA TGACGTAGAA
    AGTGAACCAG CCATGACCCC ATCCAAGGAG GGCACCCTAA TCGTCCGCCA GAGTACAGTT
    GACCAAAAGC GTGCCAGCCA TCATGAGAGC AATGGCTTTG CGGGTCGCAT TCACCTCTTG
    CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCTTC CTCCCCATCC
    TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTGCTAAT
    GAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT
    TTTATAGACC CCAGGTTACT ACAGATCTCT CCATCTAGTG GAACAACAGT TACTTCCGTT
    GTGGGATTTT CCTGTGATGG GCTGAGACCA GAAGCCATAA GGCAAGATCC TACTCGGAAA
    GGCTCAGTGG TCAATGTGAA CCCCACGAAC ACTAGGCCAC AGAGTGATAC CCCCGAGATT
    CGTAAATACA AGAAGAGATT TAACTCAGAG ATCCTGTGTG CTGCCTTATG GGGAGTGAAT
    TTGCTAGTTG GCACAGAGAG TGGCCTGATG CTCTTGGACA GAAGTGGCCA AGGGAAAGTG
    TACCCTCTGA TCAGCCGGAG ACGGTTCCAG CAAATGGATG TACTTGAGGG CCTGAATGTC
    CTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGCGTCT ACTATTTATC CTGGTTAAGA
    AATAAAATAC TTCATAATGA TCCGGAAGTT GAGAAGAAGC AGGGGTGGAC CACCGTGGGC
    GACCTGGAAG GGTGCGTGCA CTATAAAGTC GTAAAATATG AAAGAATCAA GTTTCTGGTA
    ATCGCTTTGA AGAGTTCTGT GGAAGTCTAT GCATGGGCAC CGAAGCCATA TCACAAATTT
    ATGGCCTTTA AGTCATTTGG AGAACTGGTA CATAAGCCAT TACTGGTGGA TCTCACTGTC
    GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT
    GTGGATTCCG GCTCAGTCTA TGACATTTAT CTACCAACAC ACGTAAGAAA GAACCCACAC
    TCTATGATTC AGTGTACCAT CAAACCCCAT GCAATCATTA TCCTCCCCAA CACGGATGGA
    ATGGAGCTTC TGGTGTGCTA CGAAGATGAA GGGGTTTATG TGAACACGTA CGGAAGAATC
    ACCAAGGACG TGGTTCTGCA GTGGGGAGAG ATGCCAACAT CTGTAGCATA CATCCGGTCG
    AATCAGACAA TGGGCTGGGG AGAGAAGGCA ATAGAGATAC GCTCTGTGGA AACTGGTCAT
    TTGGACGGTG TGTTTATGCA CAAAAGGGCT CAGAGACTAA AGTTCCTGTG TGAACGTAAT
    GACAAGGTGT TCTTTGCTTC CGTCCGCTCT GGCGGCAGCA GCCAGGTCTA CTTCATGACC
    TTAGGCAGGA CTTCCCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765223.2
    CDS211..4380
    Translation

    Target ORF information:

    RefSeq Version XM_008767001.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767001.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT
    TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG
    CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGTA TTGAGCCCCG GCTTCTATGG
    GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC
    TCAGGATCCC AGCCTGGCTC CCATCCTGGG TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA
    GTGAGATCAT CATCCAAGTC TGAAGGTTCT CCATCTCAGC GCCTTGAAAA TGCAGCCAAA
    AAACCTGAAG ATAAGAAAGA AGTATTTCGA CCTCTCAAAC CTGCTGGAGA AGTGGACTTG
    ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTGC GGCCACCTCA CAAAGTGACA
    GACTATTCCT CCTCCAGTGA GGAGTCTGGG ACCACGGATG AGGAGGAGGA GGATGTGGAA
    CAGGAGGGGG CTGATGATTC CACCTCGGGG CCAGAGGACA CCAGAGCAGC GTCGTCTCTG
    AATCTGAGCA ACGGTGAGAC AGAATCTGTG AAAACAATGA TTGTTCATGA TGACGTAGAA
    AGTGAACCAG CCATGACCCC ATCCAAGGAG GGCACCCTAA TCGTCCGCCA GAGTACAGTT
    GACCAAAAGC GTGCCAGCCA TCATGAGAGC AATGGCTTTG CGGGTCGCAT TCACCTCTTG
    CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCTTC CTCCCCATCC
    TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTGCTAAT
    GAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT
    TTTATAGACC CCAGGTTACT ACAGATCTCT CCATCTAGTG GAACAACAGT TACTTCCGTT
    GTGGGATTTT CCTGTGATGG GCTGAGACCA GAAGCCATAA GGCAAGATCC TACTCGGAAA
    GGCTCAGTGG TCAATGTGAA CCCCACGAAC ACTAGGCCAC AGAGTGATAC CCCCGAGATT
    CGTAAATACA AGAAGAGATT TAACTCAGAG ATCCTGTGTG CTGCCTTATG GGGAGTGAAT
    TTGCTAGTTG GCACAGAGAG TGGCCTGATG CTCTTGGACA GAAGTGGCCA AGGGAAAGTG
    TACCCTCTGA TCAGCCGGAG ACGGTTCCAG CAAATGGATG TACTTGAGGG CCTGAATGTC
    CTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGCGTCT ACTATTTATC CTGGTTAAGA
    AATAAAATAC TTCATAATGA TCCGGAAGTT GAGAAGAAGC AGGGGTGGAC CACCGTGGGC
    GACCTGGAAG GGTGCGTGCA CTATAAAGTC GTAAAATATG AAAGAATCAA GTTTCTGGTA
    ATCGCTTTGA AGAGTTCTGT GGAAGTCTAT GCATGGGCAC CGAAGCCATA TCACAAATTT
    ATGGCCTTTA AGTCATTTGG AGAACTGGTA CATAAGCCAT TACTGGTGGA TCTCACTGTC
    GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT
    GTGGATTCCG GCTCAGTCTA TGACATTTAT CTACCAACAC ACGTAAGAAA GAACCCACAC
    TCTATGATTC AGTGTACCAT CAAACCCCAT GCAATCATTA TCCTCCCCAA CACGGATGGA
    ATGGAGCTTC TGGTGTGCTA CGAAGATGAA GGGGTTTATG TGAACACGTA CGGAAGAATC
    ACCAAGGACG TGGTTCTGCA GTGGGGAGAG ATGCCAACAT CTGTAGCATA CATCCGGTCG
    AATCAGACAA TGGGCTGGGG AGAGAAGGCA ATAGAGATAC GCTCTGTGGA AACTGGTCAT
    TTGGACGGTG TGTTTATGCA CAAAAGGGCT CAGAGACTAA AGTTCCTGTG TGAACGTAAT
    GACAAGGTGT TCTTTGCTTC CGTCCGCTCT GGCGGCAGCA GCCAGGTCTA CTTCATGACC
    TTAGGCAGGA CTTCCCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62692
    Clone ID Related Accession (Same CDS sequence) XM_008767002.2
    Accession Version XM_008767002.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767002.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    TGGTCCCACC TGGCATCTCT CAAGAACAAT GTCTCCCCTG TCTCGAGATC CCATTCCTTC
    AGTGACCCTT CTCCCAAATT CGCACACCAC CATCTCCGCT CTCAGGACCC ATGTCCACCT
    TCCCGCAGTG AGGGGCTCAG TCAGAGCTCT GACTCTAAGT CGGAGGTACC TGAGCCCACC
    CAAAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CAAGGGTTCC TGTGAGAACG
    ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA
    AATAGCCAAG CAGGACAGAG AAATTCCACC AGCAGTATTG AGCCCCGGCT TCTATGGGAG
    AGAGTGGAAA AGCTGGTCCC CAGGCCAGGC AGTGGCAGCT CTTCAGGGTC CAGCAACTCA
    GGATCCCAGC CTGGCTCCCA TCCTGGGTCT CAGAGTGGCT CTGGTGAACG CTTCAGAGTG
    AGATCATCAT CCAAGTCTGA AGGTTCTCCA TCTCAGCGCC TTGAAAATGC AGCCAAAAAA
    CCTGAAGATA AGAAAGAAGT ATTTCGACCT CTCAAACCTG CTGGAGAAGT GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTGCGGC CACCTCACAA AGTGACAGAC
    TATTCCTCCT CCAGTGAGGA GTCTGGGACC ACGGATGAGG AGGAGGAGGA TGTGGAACAG
    GAGGGGGCTG ATGATTCCAC CTCGGGGCCA GAGGACACCA GAGCAGCGTC GTCTCTGAAT
    CTGAGCAACG GTGAGACAGA ATCTGTGAAA ACAATGATTG TTCATGATGA CGTAGAAAGT
    GAACCAGCCA TGACCCCATC CAAGGAGGGC ACCCTAATCG TCCGCCAGAG TACAGTTGAC
    CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA
    GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCTTCCTC CCCATCCTCC
    AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG
    ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT
    ATAGACCCCA GGTTACTACA GATCTCTCCA TCTAGTGGAA CAACAGTTAC TTCCGTTGTG
    GGATTTTCCT GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC
    TCAGTGGTCA ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT
    AAATACAAGA AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAATTTG
    CTAGTTGGCA CAGAGAGTGG CCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTGTAC
    CCTCTGATCA GCCGGAGACG GTTCCAGCAA ATGGATGTAC TTGAGGGCCT GAATGTCCTG
    GTGACAATAT CTGGCAAAAA GGATAAGTTA CGCGTCTACT ATTTATCCTG GTTAAGAAAT
    AAAATACTTC ATAATGATCC GGAAGTTGAG AAGAAGCAGG GGTGGACCAC CGTGGGCGAC
    CTGGAAGGGT GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATC
    GCTTTGAAGA GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG
    GCCTTTAAGT CATTTGGAGA ACTGGTACAT AAGCCATTAC TGGTGGATCT CACTGTCGAG
    GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG
    GATTCCGGCT CAGTCTATGA CATTTATCTA CCAACACACG TAAGAAAGAA CCCACACTCT
    ATGATTCAGT GTACCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGAATG
    GAGCTTCTGG TGTGCTACGA AGATGAAGGG GTTTATGTGA ACACGTACGG AAGAATCACC
    AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCAACATCTG TAGCATACAT CCGGTCGAAT
    CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGCT CTGTGGAAAC TGGTCATTTG
    GACGGTGTGT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGTAATGAC
    AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC GGCAGCAGCC AGGTCTACTT CATGACCTTA
    GGCAGGACTT CCCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765224.2
    CDS211..4377
    Translation

    Target ORF information:

    RefSeq Version XM_008767002.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767002.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    TGGTCCCACC TGGCATCTCT CAAGAACAAT GTCTCCCCTG TCTCGAGATC CCATTCCTTC
    AGTGACCCTT CTCCCAAATT CGCACACCAC CATCTCCGCT CTCAGGACCC ATGTCCACCT
    TCCCGCAGTG AGGGGCTCAG TCAGAGCTCT GACTCTAAGT CGGAGGTACC TGAGCCCACC
    CAAAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CAAGGGTTCC TGTGAGAACG
    ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA
    AATAGCCAAG CAGGACAGAG AAATTCCACC AGCAGTATTG AGCCCCGGCT TCTATGGGAG
    AGAGTGGAAA AGCTGGTCCC CAGGCCAGGC AGTGGCAGCT CTTCAGGGTC CAGCAACTCA
    GGATCCCAGC CTGGCTCCCA TCCTGGGTCT CAGAGTGGCT CTGGTGAACG CTTCAGAGTG
    AGATCATCAT CCAAGTCTGA AGGTTCTCCA TCTCAGCGCC TTGAAAATGC AGCCAAAAAA
    CCTGAAGATA AGAAAGAAGT ATTTCGACCT CTCAAACCTG CTGGAGAAGT GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTGCGGC CACCTCACAA AGTGACAGAC
    TATTCCTCCT CCAGTGAGGA GTCTGGGACC ACGGATGAGG AGGAGGAGGA TGTGGAACAG
    GAGGGGGCTG ATGATTCCAC CTCGGGGCCA GAGGACACCA GAGCAGCGTC GTCTCTGAAT
    CTGAGCAACG GTGAGACAGA ATCTGTGAAA ACAATGATTG TTCATGATGA CGTAGAAAGT
    GAACCAGCCA TGACCCCATC CAAGGAGGGC ACCCTAATCG TCCGCCAGAG TACAGTTGAC
    CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA
    GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCTTCCTC CCCATCCTCC
    AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG
    ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT
    ATAGACCCCA GGTTACTACA GATCTCTCCA TCTAGTGGAA CAACAGTTAC TTCCGTTGTG
    GGATTTTCCT GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC
    TCAGTGGTCA ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT
    AAATACAAGA AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAATTTG
    CTAGTTGGCA CAGAGAGTGG CCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTGTAC
    CCTCTGATCA GCCGGAGACG GTTCCAGCAA ATGGATGTAC TTGAGGGCCT GAATGTCCTG
    GTGACAATAT CTGGCAAAAA GGATAAGTTA CGCGTCTACT ATTTATCCTG GTTAAGAAAT
    AAAATACTTC ATAATGATCC GGAAGTTGAG AAGAAGCAGG GGTGGACCAC CGTGGGCGAC
    CTGGAAGGGT GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATC
    GCTTTGAAGA GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG
    GCCTTTAAGT CATTTGGAGA ACTGGTACAT AAGCCATTAC TGGTGGATCT CACTGTCGAG
    GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG
    GATTCCGGCT CAGTCTATGA CATTTATCTA CCAACACACG TAAGAAAGAA CCCACACTCT
    ATGATTCAGT GTACCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGAATG
    GAGCTTCTGG TGTGCTACGA AGATGAAGGG GTTTATGTGA ACACGTACGG AAGAATCACC
    AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCAACATCTG TAGCATACAT CCGGTCGAAT
    CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGCT CTGTGGAAAC TGGTCATTTG
    GACGGTGTGT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGTAATGAC
    AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC GGCAGCAGCC AGGTCTACTT CATGACCTTA
    GGCAGGACTT CCCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62693
    Clone ID Related Accession (Same CDS sequence) XM_008767003.2
    Accession Version XM_008767003.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT
    TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG
    CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA
    TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC
    AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGA CTTGACTGCG
    TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTGCGGCCAC CTCACAAAGT GACAGACTAT
    TCCTCCTCCA GTGAGGAGTC TGGGACCACG GATGAGGAGG AGGAGGATGT GGAACAGGAG
    GGGGCTGATG ATTCCACCTC GGGGCCAGAG GACACCAGAG CAGCGTCGTC TCTGAATCTG
    AGCAACGGTG AGACAGAATC TGTGAAAACA ATGATTGTTC ATGATGACGT AGAAAGTGAA
    CCAGCCATGA CCCCATCCAA GGAGGGCACC CTAATCGTCC GCCAGAGTAC AGTTGACCAA
    AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CTTCCTCCCC ATCCTCCAGC
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGGT TACTACAGAT CTCTCCATCT AGTGGAACAA CAGTTACTTC CGTTGTGGGA
    TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC ATAAGGCAAG ATCCTACTCG GAAAGGCTCA
    GTGGTCAATG TGAACCCCAC GAACACTAGG CCACAGAGTG ATACCCCCGA GATTCGTAAA
    TACAAGAAGA GATTTAACTC AGAGATCCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA
    GTTGGCACAG AGAGTGGCCT GATGCTCTTG GACAGAAGTG GCCAAGGGAA AGTGTACCCT
    CTGATCAGCC GGAGACGGTT CCAGCAAATG GATGTACTTG AGGGCCTGAA TGTCCTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGC GTCTACTATT TATCCTGGTT AAGAAATAAA
    ATACTTCATA ATGATCCGGA AGTTGAGAAG AAGCAGGGGT GGACCACCGT GGGCGACCTG
    GAAGGGTGCG TGCACTATAA AGTCGTAAAA TATGAAAGAA TCAAGTTTCT GGTAATCGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCATGG GCACCGAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAACT GGTACATAAG CCATTACTGG TGGATCTCAC TGTCGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT
    TCCGGCTCAG TCTATGACAT TTATCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG
    ATTCAGTGTA CCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGAATGGAG
    CTTCTGGTGT GCTACGAAGA TGAAGGGGTT TATGTGAACA CGTACGGAAG AATCACCAAG
    GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATACATCCG GTCGAATCAG
    ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGCTCTG TGGAAACTGG TCATTTGGAC
    GGTGTGTTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG TAATGACAAG
    GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC AGCAGCCAGG TCTACTTCAT GACCTTAGGC
    AGGACTTCCC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765225.2
    CDS210..4373
    Translation

    Target ORF information:

    RefSeq Version XM_008767003.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767003.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT
    TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG
    CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA
    TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC
    AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGA CTTGACTGCG
    TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTGCGGCCAC CTCACAAAGT GACAGACTAT
    TCCTCCTCCA GTGAGGAGTC TGGGACCACG GATGAGGAGG AGGAGGATGT GGAACAGGAG
    GGGGCTGATG ATTCCACCTC GGGGCCAGAG GACACCAGAG CAGCGTCGTC TCTGAATCTG
    AGCAACGGTG AGACAGAATC TGTGAAAACA ATGATTGTTC ATGATGACGT AGAAAGTGAA
    CCAGCCATGA CCCCATCCAA GGAGGGCACC CTAATCGTCC GCCAGAGTAC AGTTGACCAA
    AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CTTCCTCCCC ATCCTCCAGC
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGGT TACTACAGAT CTCTCCATCT AGTGGAACAA CAGTTACTTC CGTTGTGGGA
    TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC ATAAGGCAAG ATCCTACTCG GAAAGGCTCA
    GTGGTCAATG TGAACCCCAC GAACACTAGG CCACAGAGTG ATACCCCCGA GATTCGTAAA
    TACAAGAAGA GATTTAACTC AGAGATCCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA
    GTTGGCACAG AGAGTGGCCT GATGCTCTTG GACAGAAGTG GCCAAGGGAA AGTGTACCCT
    CTGATCAGCC GGAGACGGTT CCAGCAAATG GATGTACTTG AGGGCCTGAA TGTCCTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGC GTCTACTATT TATCCTGGTT AAGAAATAAA
    ATACTTCATA ATGATCCGGA AGTTGAGAAG AAGCAGGGGT GGACCACCGT GGGCGACCTG
    GAAGGGTGCG TGCACTATAA AGTCGTAAAA TATGAAAGAA TCAAGTTTCT GGTAATCGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCATGG GCACCGAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAACT GGTACATAAG CCATTACTGG TGGATCTCAC TGTCGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT
    TCCGGCTCAG TCTATGACAT TTATCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG
    ATTCAGTGTA CCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGAATGGAG
    CTTCTGGTGT GCTACGAAGA TGAAGGGGTT TATGTGAACA CGTACGGAAG AATCACCAAG
    GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATACATCCG GTCGAATCAG
    ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGCTCTG TGGAAACTGG TCATTTGGAC
    GGTGTGTTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG TAATGACAAG
    GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC AGCAGCCAGG TCTACTTCAT GACCTTAGGC
    AGGACTTCCC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62694
    Clone ID Related Accession (Same CDS sequence) XM_008767004.2
    Accession Version XM_008767004.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT
    TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG
    CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA
    TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC
    AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGG AGAAGTGGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG
    ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG
    GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT
    CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA
    GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGAGTACA
    GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC TTCCTCCCCA
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT
    AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGGTT ACTACAGATC TCTCCATCTA GTGGAACAAC AGTTACTTCC
    GTTGTGGGAT TTTCCTGTGA TGGGCTGAGA CCAGAAGCCA TAAGGCAAGA TCCTACTCGG
    AAAGGCTCAG TGGTCAATGT GAACCCCACG AACACTAGGC CACAGAGTGA TACCCCCGAG
    ATTCGTAAAT ACAAGAAGAG ATTTAACTCA GAGATCCTGT GTGCTGCCTT ATGGGGAGTG
    AATTTGCTAG TTGGCACAGA GAGTGGCCTG ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA
    GTGTACCCTC TGATCAGCCG GAGACGGTTC CAGCAAATGG ATGTACTTGA GGGCCTGAAT
    GTCCTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGCG TCTACTATTT ATCCTGGTTA
    AGAAATAAAA TACTTCATAA TGATCCGGAA GTTGAGAAGA AGCAGGGGTG GACCACCGTG
    GGCGACCTGG AAGGGTGCGT GCACTATAAA GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG
    GTAATCGCTT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CACCGAAGCC ATATCACAAA
    TTTATGGCCT TTAAGTCATT TGGAGAACTG GTACATAAGC CATTACTGGT GGATCTCACT
    GTCGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT
    GATGTGGATT CCGGCTCAGT CTATGACATT TATCTACCAA CACACATTCA GTGTACCATC
    AAACCCCATG CAATCATTAT CCTCCCCAAC ACGGATGGAA TGGAGCTTCT GGTGTGCTAC
    GAAGATGAAG GGGTTTATGT GAACACGTAC GGAAGAATCA CCAAGGACGT GGTTCTGCAG
    TGGGGAGAGA TGCCAACATC TGTAGCATAC ATCCGGTCGA ATCAGACAAT GGGCTGGGGA
    GAGAAGGCAA TAGAGATACG CTCTGTGGAA ACTGGTCATT TGGACGGTGT GTTTATGCAC
    AAAAGGGCTC AGAGACTAAA GTTCCTGTGT GAACGTAATG ACAAGGTGTT CTTTGCTTCC
    GTCCGCTCTG GCGGCAGCAG CCAGGTCTAC TTCATGACCT TAGGCAGGAC TTCCCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765226.2
    CDS210..4358
    Translation

    Target ORF information:

    RefSeq Version XM_008767004.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767004.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT
    TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG
    CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA
    TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC
    AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGG AGAAGTGGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG
    ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG
    GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT
    CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA
    GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGAGTACA
    GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC TTCCTCCCCA
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT
    AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGGTT ACTACAGATC TCTCCATCTA GTGGAACAAC AGTTACTTCC
    GTTGTGGGAT TTTCCTGTGA TGGGCTGAGA CCAGAAGCCA TAAGGCAAGA TCCTACTCGG
    AAAGGCTCAG TGGTCAATGT GAACCCCACG AACACTAGGC CACAGAGTGA TACCCCCGAG
    ATTCGTAAAT ACAAGAAGAG ATTTAACTCA GAGATCCTGT GTGCTGCCTT ATGGGGAGTG
    AATTTGCTAG TTGGCACAGA GAGTGGCCTG ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA
    GTGTACCCTC TGATCAGCCG GAGACGGTTC CAGCAAATGG ATGTACTTGA GGGCCTGAAT
    GTCCTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGCG TCTACTATTT ATCCTGGTTA
    AGAAATAAAA TACTTCATAA TGATCCGGAA GTTGAGAAGA AGCAGGGGTG GACCACCGTG
    GGCGACCTGG AAGGGTGCGT GCACTATAAA GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG
    GTAATCGCTT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CACCGAAGCC ATATCACAAA
    TTTATGGCCT TTAAGTCATT TGGAGAACTG GTACATAAGC CATTACTGGT GGATCTCACT
    GTCGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT
    GATGTGGATT CCGGCTCAGT CTATGACATT TATCTACCAA CACACATTCA GTGTACCATC
    AAACCCCATG CAATCATTAT CCTCCCCAAC ACGGATGGAA TGGAGCTTCT GGTGTGCTAC
    GAAGATGAAG GGGTTTATGT GAACACGTAC GGAAGAATCA CCAAGGACGT GGTTCTGCAG
    TGGGGAGAGA TGCCAACATC TGTAGCATAC ATCCGGTCGA ATCAGACAAT GGGCTGGGGA
    GAGAAGGCAA TAGAGATACG CTCTGTGGAA ACTGGTCATT TGGACGGTGT GTTTATGCAC
    AAAAGGGCTC AGAGACTAAA GTTCCTGTGT GAACGTAATG ACAAGGTGTT CTTTGCTTCC
    GTCCGCTCTG GCGGCAGCAG CCAGGTCTAC TTCATGACCT TAGGCAGGAC TTCCCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62695
    Clone ID Related Accession (Same CDS sequence) XM_008767005.2
    Accession Version XM_008767005.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767005.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT
    AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG
    CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT
    GCCCTGCAGA GACCAGCGGA GCCACAGGTA CAGTGGTCCC ACCTGGCATC TCTCAAGAAC
    AATGTCTCCC CTGTCTCGAG ATCCCATTCC TTCAGTGACC CTTCTCCCAA ATTCGCACAC
    CACCATCTCC GCTCTCAGGA CCCATGTCCA CCTTCCCGCA GTGAGGGGCT CAGTCAGAGC
    TCTGACTCTA AGTCGGAGGT ACCTGAGCCC ACCCAAAAGG CTTGGTCTAG ATCAGACAGT
    GACGAGGTGC CTCCAAGGGT TCCTGTGAGA ACGACATCTC GTTCCCCTGT CCTGTCCCGT
    CGAGATTCTC CACTGCAGGG CAGTGGGCAG CAAAATAGCC AAGCAGGACA GAGAAATTCC
    ACCAGCAGTA TTGAGCCCCG GCTTCTATGG GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA
    GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC TCAGGATCCC AGCCTGGCTC CCATCCTGGG
    TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA GTGAGATCAT CATCCAAGTC TGAAGGTTCT
    CCATCTCAGC GCCTTGAAAA TGCAGCCAAA AAACCTGAAG ATAAGAAAGA AGTATTTCGA
    CCTCTCAAAC CTGCTGGAGA AGTGGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG
    GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG
    ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG
    CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG
    AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG
    GGCACCCTAA TCGTCCGCCA GAGTACAGTT GACCAAAAGC GTGCCAGCCA TCATGAGAGC
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC
    TCCACTTCCT CCACCTCTTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA
    CAGACACCCC AGGACAAGCT CACTGCTAAT GAGACTCAGT CCGCTAGTAG CACACTCCAG
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGGTTACT ACAGATCTCT
    CCATCTAGTG GAACAACAGT TACTTCCGTT GTGGGATTTT CCTGTGATGG GCTGAGACCA
    GAAGCCATAA GGCAAGATCC TACTCGGAAA GGCTCAGTGG TCAATGTGAA CCCCACGAAC
    ACTAGGCCAC AGAGTGATAC CCCCGAGATT CGTAAATACA AGAAGAGATT TAACTCAGAG
    ATCCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTTG GCACAGAGAG TGGCCTGATG
    CTCTTGGACA GAAGTGGCCA AGGGAAAGTG TACCCTCTGA TCAGCCGGAG ACGGTTCCAG
    CAAATGGATG TACTTGAGGG CCTGAATGTC CTGGTGACAA TATCTGGCAA AAAGGATAAG
    TTACGCGTCT ACTATTTATC CTGGTTAAGA AATAAAATAC TTCATAATGA TCCGGAAGTT
    GAGAAGAAGC AGGGGTGGAC CACCGTGGGC GACCTGGAAG GGTGCGTGCA CTATAAAGTC
    GTAAAATATG AAAGAATCAA GTTTCTGGTA ATCGCTTTGA AGAGTTCTGT GGAAGTCTAT
    GCATGGGCAC CGAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAACTGGTA
    CATAAGCCAT TACTGGTGGA TCTCACTGTC GAGGAAGGCC AGAGGTTGAA AGTGATCTAT
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCCG GCTCAGTCTA TGACATTTAT
    CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATTC AGTGTACCAT CAAACCCCAT
    GCAATCATTA TCCTCCCCAA CACGGATGGA ATGGAGCTTC TGGTGTGCTA CGAAGATGAA
    GGGGTTTATG TGAACACGTA CGGAAGAATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG
    ATGCCAACAT CTGTAGCATA CATCCGGTCG AATCAGACAA TGGGCTGGGG AGAGAAGGCA
    ATAGAGATAC GCTCTGTGGA AACTGGTCAT TTGGACGGTG TGTTTATGCA CAAAAGGGCT
    CAGAGACTAA AGTTCCTGTG TGAACGTAAT GACAAGGTGT TCTTTGCTTC CGTCCGCTCT
    GGCGGCAGCA GCCAGGTCTA CTTCATGACC TTAGGCAGGA CTTCCCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765227.2
    CDS211..4290
    Translation

    Target ORF information:

    RefSeq Version XM_008767005.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767005.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT
    AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG
    CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT
    GCCCTGCAGA GACCAGCGGA GCCACAGGTA CAGTGGTCCC ACCTGGCATC TCTCAAGAAC
    AATGTCTCCC CTGTCTCGAG ATCCCATTCC TTCAGTGACC CTTCTCCCAA ATTCGCACAC
    CACCATCTCC GCTCTCAGGA CCCATGTCCA CCTTCCCGCA GTGAGGGGCT CAGTCAGAGC
    TCTGACTCTA AGTCGGAGGT ACCTGAGCCC ACCCAAAAGG CTTGGTCTAG ATCAGACAGT
    GACGAGGTGC CTCCAAGGGT TCCTGTGAGA ACGACATCTC GTTCCCCTGT CCTGTCCCGT
    CGAGATTCTC CACTGCAGGG CAGTGGGCAG CAAAATAGCC AAGCAGGACA GAGAAATTCC
    ACCAGCAGTA TTGAGCCCCG GCTTCTATGG GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA
    GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC TCAGGATCCC AGCCTGGCTC CCATCCTGGG
    TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA GTGAGATCAT CATCCAAGTC TGAAGGTTCT
    CCATCTCAGC GCCTTGAAAA TGCAGCCAAA AAACCTGAAG ATAAGAAAGA AGTATTTCGA
    CCTCTCAAAC CTGCTGGAGA AGTGGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG
    GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG
    ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG
    CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG
    AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG
    GGCACCCTAA TCGTCCGCCA GAGTACAGTT GACCAAAAGC GTGCCAGCCA TCATGAGAGC
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC
    TCCACTTCCT CCACCTCTTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA
    CAGACACCCC AGGACAAGCT CACTGCTAAT GAGACTCAGT CCGCTAGTAG CACACTCCAG
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGGTTACT ACAGATCTCT
    CCATCTAGTG GAACAACAGT TACTTCCGTT GTGGGATTTT CCTGTGATGG GCTGAGACCA
    GAAGCCATAA GGCAAGATCC TACTCGGAAA GGCTCAGTGG TCAATGTGAA CCCCACGAAC
    ACTAGGCCAC AGAGTGATAC CCCCGAGATT CGTAAATACA AGAAGAGATT TAACTCAGAG
    ATCCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTTG GCACAGAGAG TGGCCTGATG
    CTCTTGGACA GAAGTGGCCA AGGGAAAGTG TACCCTCTGA TCAGCCGGAG ACGGTTCCAG
    CAAATGGATG TACTTGAGGG CCTGAATGTC CTGGTGACAA TATCTGGCAA AAAGGATAAG
    TTACGCGTCT ACTATTTATC CTGGTTAAGA AATAAAATAC TTCATAATGA TCCGGAAGTT
    GAGAAGAAGC AGGGGTGGAC CACCGTGGGC GACCTGGAAG GGTGCGTGCA CTATAAAGTC
    GTAAAATATG AAAGAATCAA GTTTCTGGTA ATCGCTTTGA AGAGTTCTGT GGAAGTCTAT
    GCATGGGCAC CGAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAACTGGTA
    CATAAGCCAT TACTGGTGGA TCTCACTGTC GAGGAAGGCC AGAGGTTGAA AGTGATCTAT
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCCG GCTCAGTCTA TGACATTTAT
    CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATTC AGTGTACCAT CAAACCCCAT
    GCAATCATTA TCCTCCCCAA CACGGATGGA ATGGAGCTTC TGGTGTGCTA CGAAGATGAA
    GGGGTTTATG TGAACACGTA CGGAAGAATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG
    ATGCCAACAT CTGTAGCATA CATCCGGTCG AATCAGACAA TGGGCTGGGG AGAGAAGGCA
    ATAGAGATAC GCTCTGTGGA AACTGGTCAT TTGGACGGTG TGTTTATGCA CAAAAGGGCT
    CAGAGACTAA AGTTCCTGTG TGAACGTAAT GACAAGGTGT TCTTTGCTTC CGTCCGCTCT
    GGCGGCAGCA GCCAGGTCTA CTTCATGACC TTAGGCAGGA CTTCCCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62696
    Clone ID Related Accession (Same CDS sequence) XM_008767006.2
    Accession Version XM_008767006.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT ACAGTGGTCC CACCTGGCAT CTCTCAAGAA CAATGTCTCC
    CCTGTCTCGA GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTCGCACA CCACCATCTC
    CGCTCTCAGG ACCCATGTCC ACCTTCCCGC AGTGAGGGGC TCAGTCAGAG CTCTGACTCT
    AAGTCGGAGG TACCTGAGCC CACCCAAAAG GCTTGGTCTA GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TTCCTGTGAG AACGACATCT CGTTCCCCTG TCCTGTCCCG TCGAGATTCT
    CCACTGCAGG GCAGTGGGCA GCAAAATAGC CAAGCAGGAC AGAGAAATTC CACCAGCAGT
    ATTGAGCCCC GGCTTCTATG GGAGAGAGTG GAAAAGCTGG TCCCCAGGCC AGGCAGTGGC
    AGCTCTTCAG GGTCCAGCAA CTCAGGATCC CAGCCTGGCT CCCATCCTGG GTCTCAGAGT
    GGCTCTGGTG AACGCTTCAG AGTGAGATCA TCATCCAAGT CTGAAGGTTC TCCATCTCAG
    CGCCTTGAAA ATGCAGCCAA AAAACCTGAA GATAAGAAAG AAGTATTTCG ACCTCTCAAA
    CCTGCTGGAG AAGTGGACTT GACTGCGTTG GCCAAAGAGC TTCGAGCAGT GGAAGATGTG
    CGGCCACCTC ACAAAGTGAC AGACTATTCC TCCTCCAGTG AGGAGTCTGG GACCACGGAT
    GAGGAGGAGG AGGATGTGGA ACAGGAGGGG GCTGATGATT CCACCTCGGG GCCAGAGGAC
    ACCAGAGCAG CGTCGTCTCT GAATCTGAGC AACGGTGAGA CAGAATCTGT GAAAACAATG
    ATTGTTCATG ATGACGTAGA AAGTGAACCA GCCATGACCC CATCCAAGGA GGGCACCCTA
    ATCGTCCGCC AGAGTACAGT TGACCAAAAG CGTGCCAGCC ATCATGAGAG CAATGGCTTT
    GCGGGTCGCA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACTTCC
    TCCACCTCTT CCTCCCCATC CTCCAGCCAG CCGACACCCA CCATGTCCCC ACAGACACCC
    CAGGACAAGC TCACTGCTAA TGAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGGTTAC TACAGATCTC TCCATCTAGT
    GGAACAACAG TTACTTCCGT TGTGGGATTT TCCTGTGATG GGCTGAGACC AGAAGCCATA
    AGGCAAGATC CTACTCGGAA AGGCTCAGTG GTCAATGTGA ACCCCACGAA CACTAGGCCA
    CAGAGTGATA CCCCCGAGAT TCGTAAATAC AAGAAGAGAT TTAACTCAGA GATCCTGTGT
    GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT GGCACAGAGA GTGGCCTGAT GCTCTTGGAC
    AGAAGTGGCC AAGGGAAAGT GTACCCTCTG ATCAGCCGGA GACGGTTCCA GCAAATGGAT
    GTACTTGAGG GCCTGAATGT CCTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGCGTC
    TACTATTTAT CCTGGTTAAG AAATAAAATA CTTCATAATG ATCCGGAAGT TGAGAAGAAG
    CAGGGGTGGA CCACCGTGGG CGACCTGGAA GGGTGCGTGC ACTATAAAGT CGTAAAATAT
    GAAAGAATCA AGTTTCTGGT AATCGCTTTG AAGAGTTCTG TGGAAGTCTA TGCATGGGCA
    CCGAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAACTGGT ACATAAGCCA
    TTACTGGTGG ATCTCACTGT CGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT
    GCTGGATTCC ATGCTGTTGA TGTGGATTCC GGCTCAGTCT ATGACATTTA TCTACCAACA
    CACGTAAGAA AGAACCCACA CTCTATGATT CAGTGTACCA TCAAACCCCA TGCAATCATT
    ATCCTCCCCA ACACGGATGG AATGGAGCTT CTGGTGTGCT ACGAAGATGA AGGGGTTTAT
    GTGAACACGT ACGGAAGAAT CACCAAGGAC GTGGTTCTGC AGTGGGGAGA GATGCCAACA
    TCTGTAGCAT ACATCCGGTC GAATCAGACA ATGGGCTGGG GAGAGAAGGC AATAGAGATA
    CGCTCTGTGG AAACTGGTCA TTTGGACGGT GTGTTTATGC ACAAAAGGGC TCAGAGACTA
    AAGTTCCTGT GTGAACGTAA TGACAAGGTG TTCTTTGCTT CCGTCCGCTC TGGCGGCAGC
    AGCCAGGTCT ACTTCATGAC CTTAGGCAGG ACTTCCCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765228.2
    CDS210..4220
    Translation

    Target ORF information:

    RefSeq Version XM_008767006.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767006.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT ACAGTGGTCC CACCTGGCAT CTCTCAAGAA CAATGTCTCC
    CCTGTCTCGA GATCCCATTC CTTCAGTGAC CCTTCTCCCA AATTCGCACA CCACCATCTC
    CGCTCTCAGG ACCCATGTCC ACCTTCCCGC AGTGAGGGGC TCAGTCAGAG CTCTGACTCT
    AAGTCGGAGG TACCTGAGCC CACCCAAAAG GCTTGGTCTA GATCAGACAG TGACGAGGTG
    CCTCCAAGGG TTCCTGTGAG AACGACATCT CGTTCCCCTG TCCTGTCCCG TCGAGATTCT
    CCACTGCAGG GCAGTGGGCA GCAAAATAGC CAAGCAGGAC AGAGAAATTC CACCAGCAGT
    ATTGAGCCCC GGCTTCTATG GGAGAGAGTG GAAAAGCTGG TCCCCAGGCC AGGCAGTGGC
    AGCTCTTCAG GGTCCAGCAA CTCAGGATCC CAGCCTGGCT CCCATCCTGG GTCTCAGAGT
    GGCTCTGGTG AACGCTTCAG AGTGAGATCA TCATCCAAGT CTGAAGGTTC TCCATCTCAG
    CGCCTTGAAA ATGCAGCCAA AAAACCTGAA GATAAGAAAG AAGTATTTCG ACCTCTCAAA
    CCTGCTGGAG AAGTGGACTT GACTGCGTTG GCCAAAGAGC TTCGAGCAGT GGAAGATGTG
    CGGCCACCTC ACAAAGTGAC AGACTATTCC TCCTCCAGTG AGGAGTCTGG GACCACGGAT
    GAGGAGGAGG AGGATGTGGA ACAGGAGGGG GCTGATGATT CCACCTCGGG GCCAGAGGAC
    ACCAGAGCAG CGTCGTCTCT GAATCTGAGC AACGGTGAGA CAGAATCTGT GAAAACAATG
    ATTGTTCATG ATGACGTAGA AAGTGAACCA GCCATGACCC CATCCAAGGA GGGCACCCTA
    ATCGTCCGCC AGAGTACAGT TGACCAAAAG CGTGCCAGCC ATCATGAGAG CAATGGCTTT
    GCGGGTCGCA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACTTCC
    TCCACCTCTT CCTCCCCATC CTCCAGCCAG CCGACACCCA CCATGTCCCC ACAGACACCC
    CAGGACAAGC TCACTGCTAA TGAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGGTTAC TACAGATCTC TCCATCTAGT
    GGAACAACAG TTACTTCCGT TGTGGGATTT TCCTGTGATG GGCTGAGACC AGAAGCCATA
    AGGCAAGATC CTACTCGGAA AGGCTCAGTG GTCAATGTGA ACCCCACGAA CACTAGGCCA
    CAGAGTGATA CCCCCGAGAT TCGTAAATAC AAGAAGAGAT TTAACTCAGA GATCCTGTGT
    GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT GGCACAGAGA GTGGCCTGAT GCTCTTGGAC
    AGAAGTGGCC AAGGGAAAGT GTACCCTCTG ATCAGCCGGA GACGGTTCCA GCAAATGGAT
    GTACTTGAGG GCCTGAATGT CCTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGCGTC
    TACTATTTAT CCTGGTTAAG AAATAAAATA CTTCATAATG ATCCGGAAGT TGAGAAGAAG
    CAGGGGTGGA CCACCGTGGG CGACCTGGAA GGGTGCGTGC ACTATAAAGT CGTAAAATAT
    GAAAGAATCA AGTTTCTGGT AATCGCTTTG AAGAGTTCTG TGGAAGTCTA TGCATGGGCA
    CCGAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAACTGGT ACATAAGCCA
    TTACTGGTGG ATCTCACTGT CGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT
    GCTGGATTCC ATGCTGTTGA TGTGGATTCC GGCTCAGTCT ATGACATTTA TCTACCAACA
    CACGTAAGAA AGAACCCACA CTCTATGATT CAGTGTACCA TCAAACCCCA TGCAATCATT
    ATCCTCCCCA ACACGGATGG AATGGAGCTT CTGGTGTGCT ACGAAGATGA AGGGGTTTAT
    GTGAACACGT ACGGAAGAAT CACCAAGGAC GTGGTTCTGC AGTGGGGAGA GATGCCAACA
    TCTGTAGCAT ACATCCGGTC GAATCAGACA ATGGGCTGGG GAGAGAAGGC AATAGAGATA
    CGCTCTGTGG AAACTGGTCA TTTGGACGGT GTGTTTATGC ACAAAAGGGC TCAGAGACTA
    AAGTTCCTGT GTGAACGTAA TGACAAGGTG TTCTTTGCTT CCGTCCGCTC TGGCGGCAGC
    AGCCAGGTCT ACTTCATGAC CTTAGGCAGG ACTTCCCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62697
    Clone ID Related Accession (Same CDS sequence) XM_017596340.1
    Accession Version XM_017596340.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4005bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT CTCCCCTGTC
    TCGAGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTCG CACACCACCA TCTCCGCTCT
    CAGGACCCAT GTCCACCTTC CCGCAGTGAG GGGCTCAGTC AGAGCTCTGA CTCTAAGTCG
    GAGGTACCTG AGCCCACCCA AAAGGCTTGG TCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTTCCTG TGAGAACGAC ATCTCGTTCC CCTGTCCTGT CCCGTCGAGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAGCCAAGCA GGACAGAGAA ATTCCACCAG CAGTATTGAG
    CCCCGGCTTC TATGGGAGAG AGTGGAAAAG CTGGTCCCCA GGCCAGGCAG TGGCAGCTCT
    TCAGGGTCCA GCAACTCAGG ATCCCAGCCT GGCTCCCATC CTGGGTCTCA GAGTGGCTCT
    GGTGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GTTCTCCATC TCAGCGCCTT
    GAAAATGCAG CCAAAAAACC TGAAGATAAG AAAGAAGTAT TTCGACCTCT CAAACCTGCT
    GGAGAAGTGG ACTTGACTGC GTTGGCCAAA GAGCTTCGAG CAGTGGAAGA TGTGCGGCCA
    CCTCACAAAG TGACAGACTA TTCCTCCTCC AGTGAGGAGT CTGGGACCAC GGATGAGGAG
    GAGGAGGATG TGGAACAGGA GGGGGCTGAT GATTCCACCT CGGGGCCAGA GGACACCAGA
    GCAGCGTCGT CTCTGAATCT GAGCAACGGT GAGACAGAAT CTGTGAAAAC AATGATTGTT
    CATGATGACG TAGAAAGTGA ACCAGCCATG ACCCCATCCA AGGAGGGCAC CCTAATCGTC
    CGCCAGAGTA CAGTTGACCA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCGGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCTTCCTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTCACTG CTAATGAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGG TTACTACAGA TCTCTCCATC TAGTGGAACA
    ACAGTTACTT CCGTTGTGGG ATTTTCCTGT GATGGGCTGA GACCAGAAGC CATAAGGCAA
    GATCCTACTC GGAAAGGCTC AGTGGTCAAT GTGAACCCCA CGAACACTAG GCCACAGAGT
    GATACCCCCG AGATTCGTAA ATACAAGAAG AGATTTAACT CAGAGATCCT GTGTGCTGCC
    TTATGGGGAG TGAATTTGCT AGTTGGCACA GAGAGTGGCC TGATGCTCTT GGACAGAAGT
    GGCCAAGGGA AAGTGTACCC TCTGATCAGC CGGAGACGGT TCCAGCAAAT GGATGTACTT
    GAGGGCCTGA ATGTCCTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG CGTCTACTAT
    TTATCCTGGT TAAGAAATAA AATACTTCAT AATGATCCGG AAGTTGAGAA GAAGCAGGGG
    TGGACCACCG TGGGCGACCT GGAAGGGTGC GTGCACTATA AAGTCGTAAA ATATGAAAGA
    ATCAAGTTTC TGGTAATCGC TTTGAAGAGT TCTGTGGAAG TCTATGCATG GGCACCGAAG
    CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAC TGGTACATAA GCCATTACTG
    GTGGATCTCA CTGTCGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA
    TTCCATGCTG TTGATGTGGA TTCCGGCTCA GTCTATGACA TTTATCTACC AACACACGTA
    AGAAAGAACC CACACTCTAT GATTCAGTGT ACCATCAAAC CCCATGCAAT CATTATCCTC
    CCCAACACGG ATGGAATGGA GCTTCTGGTG TGCTACGAAG ATGAAGGGGT TTATGTGAAC
    ACGTACGGAA GAATCACCAA GGACGTGGTT CTGCAGTGGG GAGAGATGCC AACATCTGTA
    GCATACATCC GGTCGAATCA GACAATGGGC TGGGGAGAGA AGGCAATAGA GATACGCTCT
    GTGGAAACTG GTCATTTGGA CGGTGTGTTT ATGCACAAAA GGGCTCAGAG ACTAAAGTTC
    CTGTGTGAAC GTAATGACAA GGTGTTCTTT GCTTCCGTCC GCTCTGGCGG CAGCAGCCAG
    GTCTACTTCA TGACCTTAGG CAGGACTTCC CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017451829.1
    CDS210..4214
    Translation

    Target ORF information:

    RefSeq Version XM_017596340.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017596340.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT CTCCCCTGTC
    TCGAGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTCG CACACCACCA TCTCCGCTCT
    CAGGACCCAT GTCCACCTTC CCGCAGTGAG GGGCTCAGTC AGAGCTCTGA CTCTAAGTCG
    GAGGTACCTG AGCCCACCCA AAAGGCTTGG TCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTTCCTG TGAGAACGAC ATCTCGTTCC CCTGTCCTGT CCCGTCGAGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAGCCAAGCA GGACAGAGAA ATTCCACCAG CAGTATTGAG
    CCCCGGCTTC TATGGGAGAG AGTGGAAAAG CTGGTCCCCA GGCCAGGCAG TGGCAGCTCT
    TCAGGGTCCA GCAACTCAGG ATCCCAGCCT GGCTCCCATC CTGGGTCTCA GAGTGGCTCT
    GGTGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GTTCTCCATC TCAGCGCCTT
    GAAAATGCAG CCAAAAAACC TGAAGATAAG AAAGAAGTAT TTCGACCTCT CAAACCTGCT
    GGAGAAGTGG ACTTGACTGC GTTGGCCAAA GAGCTTCGAG CAGTGGAAGA TGTGCGGCCA
    CCTCACAAAG TGACAGACTA TTCCTCCTCC AGTGAGGAGT CTGGGACCAC GGATGAGGAG
    GAGGAGGATG TGGAACAGGA GGGGGCTGAT GATTCCACCT CGGGGCCAGA GGACACCAGA
    GCAGCGTCGT CTCTGAATCT GAGCAACGGT GAGACAGAAT CTGTGAAAAC AATGATTGTT
    CATGATGACG TAGAAAGTGA ACCAGCCATG ACCCCATCCA AGGAGGGCAC CCTAATCGTC
    CGCCAGAGTA CAGTTGACCA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCGGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCTTCCTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTCACTG CTAATGAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGG TTACTACAGA TCTCTCCATC TAGTGGAACA
    ACAGTTACTT CCGTTGTGGG ATTTTCCTGT GATGGGCTGA GACCAGAAGC CATAAGGCAA
    GATCCTACTC GGAAAGGCTC AGTGGTCAAT GTGAACCCCA CGAACACTAG GCCACAGAGT
    GATACCCCCG AGATTCGTAA ATACAAGAAG AGATTTAACT CAGAGATCCT GTGTGCTGCC
    TTATGGGGAG TGAATTTGCT AGTTGGCACA GAGAGTGGCC TGATGCTCTT GGACAGAAGT
    GGCCAAGGGA AAGTGTACCC TCTGATCAGC CGGAGACGGT TCCAGCAAAT GGATGTACTT
    GAGGGCCTGA ATGTCCTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG CGTCTACTAT
    TTATCCTGGT TAAGAAATAA AATACTTCAT AATGATCCGG AAGTTGAGAA GAAGCAGGGG
    TGGACCACCG TGGGCGACCT GGAAGGGTGC GTGCACTATA AAGTCGTAAA ATATGAAAGA
    ATCAAGTTTC TGGTAATCGC TTTGAAGAGT TCTGTGGAAG TCTATGCATG GGCACCGAAG
    CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAC TGGTACATAA GCCATTACTG
    GTGGATCTCA CTGTCGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA
    TTCCATGCTG TTGATGTGGA TTCCGGCTCA GTCTATGACA TTTATCTACC AACACACGTA
    AGAAAGAACC CACACTCTAT GATTCAGTGT ACCATCAAAC CCCATGCAAT CATTATCCTC
    CCCAACACGG ATGGAATGGA GCTTCTGGTG TGCTACGAAG ATGAAGGGGT TTATGTGAAC
    ACGTACGGAA GAATCACCAA GGACGTGGTT CTGCAGTGGG GAGAGATGCC AACATCTGTA
    GCATACATCC GGTCGAATCA GACAATGGGC TGGGGAGAGA AGGCAATAGA GATACGCTCT
    GTGGAAACTG GTCATTTGGA CGGTGTGTTT ATGCACAAAA GGGCTCAGAG ACTAAAGTTC
    CTGTGTGAAC GTAATGACAA GGTGTTCTTT GCTTCCGTCC GCTCTGGCGG CAGCAGCCAG
    GTCTACTTCA TGACCTTAGG CAGGACTTCC CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62698
    Clone ID Related Accession (Same CDS sequence) XM_008767007.2
    Accession Version XM_008767007.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3981bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767007.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT
    TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG
    CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA
    TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC
    AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGG AGAAGTGGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG
    ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG
    GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT
    CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA
    GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGGTTAC TACAGATCTC TCCATCTAGT GGAACAACAG TTACTTCCGT TGTGGGATTT
    TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG
    GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC
    AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT
    GGCACAGAGA GTGGCCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT GTACCCTCTG
    ATCAGCCGGA GACGGTTCCA GCAAATGGAT GTACTTGAGG GCCTGAATGT CCTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGCGTC TACTATTTAT CCTGGTTAAG AAATAAAATA
    CTTCATAATG ATCCGGAAGT TGAGAAGAAG CAGGGGTGGA CCACCGTGGG CGACCTGGAA
    GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATCGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAACTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT CGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCC
    GGCTCAGTCT ATGACATTTA TCTACCAACA CACGTAAGAA AGAACCCACA CTCTATGATT
    CAGTGTACCA TCAAACCCCA TGCAATCATT ATCCTCCCCA ACACGGATGG AATGGAGCTT
    CTGGTGTGCT ACGAAGATGA AGGGGTTTAT GTGAACACGT ACGGAAGAAT CACCAAGGAC
    GTGGTTCTGC AGTGGGGAGA GATGCCAACA TCTGTAGCAT ACATCCGGTC GAATCAGACA
    ATGGGCTGGG GAGAGAAGGC AATAGAGATA CGCTCTGTGG AAACTGGTCA TTTGGACGGT
    GTGTTTATGC ACAAAAGGGC TCAGAGACTA AAGTTCCTGT GTGAACGTAA TGACAAGGTG
    TTCTTTGCTT CCGTCCGCTC TGGCGGCAGC AGCCAGGTCT ACTTCATGAC CTTAGGCAGG
    ACTTCCCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765229.2
    CDS211..4191
    Translation

    Target ORF information:

    RefSeq Version XM_008767007.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767007.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT
    TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG
    CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA
    TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC
    AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGG AGAAGTGGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG
    ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG
    GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT
    CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA
    GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGGTTAC TACAGATCTC TCCATCTAGT GGAACAACAG TTACTTCCGT TGTGGGATTT
    TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG
    GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC
    AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT
    GGCACAGAGA GTGGCCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT GTACCCTCTG
    ATCAGCCGGA GACGGTTCCA GCAAATGGAT GTACTTGAGG GCCTGAATGT CCTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGCGTC TACTATTTAT CCTGGTTAAG AAATAAAATA
    CTTCATAATG ATCCGGAAGT TGAGAAGAAG CAGGGGTGGA CCACCGTGGG CGACCTGGAA
    GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATCGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAACTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT CGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCC
    GGCTCAGTCT ATGACATTTA TCTACCAACA CACGTAAGAA AGAACCCACA CTCTATGATT
    CAGTGTACCA TCAAACCCCA TGCAATCATT ATCCTCCCCA ACACGGATGG AATGGAGCTT
    CTGGTGTGCT ACGAAGATGA AGGGGTTTAT GTGAACACGT ACGGAAGAAT CACCAAGGAC
    GTGGTTCTGC AGTGGGGAGA GATGCCAACA TCTGTAGCAT ACATCCGGTC GAATCAGACA
    ATGGGCTGGG GAGAGAAGGC AATAGAGATA CGCTCTGTGG AAACTGGTCA TTTGGACGGT
    GTGTTTATGC ACAAAAGGGC TCAGAGACTA AAGTTCCTGT GTGAACGTAA TGACAAGGTG
    TTCTTTGCTT CCGTCCGCTC TGGCGGCAGC AGCCAGGTCT ACTTCATGAC CTTAGGCAGG
    ACTTCCCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62699
    Clone ID Related Accession (Same CDS sequence) XM_008767008.2
    Accession Version XM_008767008.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3957bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT
    TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG
    CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA
    TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC
    AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGG AGAAGTGGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG
    ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG
    GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT
    CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA
    GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGGTTAC TACAGATCTC TCCATCTAGT GGAACAACAG TTACTTCCGT TGTGGGATTT
    TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG
    GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC
    AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT
    GGCACAGAGA GTGGCCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT GTACCCTCTG
    ATCAGCCGGA GACGGTTCCA GCAAATGGAT GTACTTGAGG GCCTGAATGT CCTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGCGTC TACTATTTAT CCTGGTTAAG AAATAAAATA
    CTTCATAATG ATCCGGAAGT TGAGAAGAAG CAGGGGTGGA CCACCGTGGG CGACCTGGAA
    GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATCGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAACTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT CGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCC
    GGCTCAGTCT ATGACATTTA TCTACCAACA CACATTCAGT GTACCATCAA ACCCCATGCA
    ATCATTATCC TCCCCAACAC GGATGGAATG GAGCTTCTGG TGTGCTACGA AGATGAAGGG
    GTTTATGTGA ACACGTACGG AAGAATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG
    CCAACATCTG TAGCATACAT CCGGTCGAAT CAGACAATGG GCTGGGGAGA GAAGGCAATA
    GAGATACGCT CTGTGGAAAC TGGTCATTTG GACGGTGTGT TTATGCACAA AAGGGCTCAG
    AGACTAAAGT TCCTGTGTGA ACGTAATGAC AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC
    GGCAGCAGCC AGGTCTACTT CATGACCTTA GGCAGGACTT CCCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765230.2
    CDS210..4166
    Translation

    Target ORF information:

    RefSeq Version XM_008767008.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767008.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT
    TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG
    CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA
    TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC
    AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGG AGAAGTGGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG
    ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG
    GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT
    CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA
    GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGGTTAC TACAGATCTC TCCATCTAGT GGAACAACAG TTACTTCCGT TGTGGGATTT
    TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG
    GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC
    AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT
    GGCACAGAGA GTGGCCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT GTACCCTCTG
    ATCAGCCGGA GACGGTTCCA GCAAATGGAT GTACTTGAGG GCCTGAATGT CCTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGCGTC TACTATTTAT CCTGGTTAAG AAATAAAATA
    CTTCATAATG ATCCGGAAGT TGAGAAGAAG CAGGGGTGGA CCACCGTGGG CGACCTGGAA
    GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATCGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAACTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT CGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCC
    GGCTCAGTCT ATGACATTTA TCTACCAACA CACATTCAGT GTACCATCAA ACCCCATGCA
    ATCATTATCC TCCCCAACAC GGATGGAATG GAGCTTCTGG TGTGCTACGA AGATGAAGGG
    GTTTATGTGA ACACGTACGG AAGAATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG
    CCAACATCTG TAGCATACAT CCGGTCGAAT CAGACAATGG GCTGGGGAGA GAAGGCAATA
    GAGATACGCT CTGTGGAAAC TGGTCATTTG GACGGTGTGT TTATGCACAA AAGGGCTCAG
    AGACTAAAGT TCCTGTGTGA ACGTAATGAC AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC
    GGCAGCAGCC AGGTCTACTT CATGACCTTA GGCAGGACTT CCCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62700
    Clone ID Related Accession (Same CDS sequence) XM_008767009.2
    Accession Version XM_008767009.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    GTTCCTGTGA GAACGACATC TCGTTCCCCT GTCCTGTCCC GTCGAGATTC TCCACTGCAG
    GGCAGTGGGC AGCAAAATAG CCAAGCAGGA CAGAGAAATT CCACCAGCAG TATTGAGCCC
    CGGCTTCTAT GGGAGAGAGT GGAAAAGCTG GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA
    GGGTCCAGCA ACTCAGGATC CCAGCCTGGC TCCCATCCTG GGTCTCAGAG TGGCTCTGGT
    GAACGCTTCA GAGTGAGATC ATCATCCAAG TCTGAAGGTT CTCCATCTCA GCGCCTTGAA
    AATGCAGCCA AAAAACCTGA AGATAAGAAA GAAGTATTTC GACCTCTCAA ACCTGCTGGA
    GAAGTGGACT TGACTGCGTT GGCCAAAGAG CTTCGAGCAG TGGAAGATGT GCGGCCACCT
    CACAAAGTGA CAGACTATTC CTCCTCCAGT GAGGAGTCTG GGACCACGGA TGAGGAGGAG
    GAGGATGTGG AACAGGAGGG GGCTGATGAT TCCACCTCGG GGCCAGAGGA CACCAGAGCA
    GCGTCGTCTC TGAATCTGAG CAACGGTGAG ACAGAATCTG TGAAAACAAT GATTGTTCAT
    GATGACGTAG AAAGTGAACC AGCCATGACC CCATCCAAGG AGGGCACCCT AATCGTCCGC
    CAGAGTACAG TTGACCAAAA GCGTGCCAGC CATCATGAGA GCAATGGCTT TGCGGGTCGC
    ATTCACCTCT TGCCAGATCT CTTACAGCAA AGCCATTCCT CCTCCACTTC CTCCACCTCT
    TCCTCCCCAT CCTCCAGCCA GCCGACACCC ACCATGTCCC CACAGACACC CCAGGACAAG
    CTCACTGCTA ATGAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC
    TCCTTTACAC CTTTTATAGA CCCCAGGTTA CTACAGATCT CTCCATCTAG TGGAACAACA
    GTTACTTCCG TTGTGGGATT TTCCTGTGAT GGGCTGAGAC CAGAAGCCAT AAGGCAAGAT
    CCTACTCGGA AAGGCTCAGT GGTCAATGTG AACCCCACGA ACACTAGGCC ACAGAGTGAT
    ACCCCCGAGA TTCGTAAATA CAAGAAGAGA TTTAACTCAG AGATCCTGTG TGCTGCCTTA
    TGGGGAGTGA ATTTGCTAGT TGGCACAGAG AGTGGCCTGA TGCTCTTGGA CAGAAGTGGC
    CAAGGGAAAG TGTACCCTCT GATCAGCCGG AGACGGTTCC AGCAAATGGA TGTACTTGAG
    GGCCTGAATG TCCTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGCGT CTACTATTTA
    TCCTGGTTAA GAAATAAAAT ACTTCATAAT GATCCGGAAG TTGAGAAGAA GCAGGGGTGG
    ACCACCGTGG GCGACCTGGA AGGGTGCGTG CACTATAAAG TCGTAAAATA TGAAAGAATC
    AAGTTTCTGG TAATCGCTTT GAAGAGTTCT GTGGAAGTCT ATGCATGGGC ACCGAAGCCA
    TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAACTGG TACATAAGCC ATTACTGGTG
    GATCTCACTG TCGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC
    CATGCTGTTG ATGTGGATTC CGGCTCAGTC TATGACATTT ATCTACCAAC ACACGTAAGA
    AAGAACCCAC ACTCTATGAT TCAGTGTACC ATCAAACCCC ATGCAATCAT TATCCTCCCC
    AACACGGATG GAATGGAGCT TCTGGTGTGC TACGAAGATG AAGGGGTTTA TGTGAACACG
    TACGGAAGAA TCACCAAGGA CGTGGTTCTG CAGTGGGGAG AGATGCCAAC ATCTGTAGCA
    TACATCCGGT CGAATCAGAC AATGGGCTGG GGAGAGAAGG CAATAGAGAT ACGCTCTGTG
    GAAACTGGTC ATTTGGACGG TGTGTTTATG CACAAAAGGG CTCAGAGACT AAAGTTCCTG
    TGTGAACGTA ATGACAAGGT GTTCTTTGCT TCCGTCCGCT CTGGCGGCAG CAGCCAGGTC
    TACTTCATGA CCTTAGGCAG GACTTCCCTT CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765231.2
    CDS210..4151
    Translation

    Target ORF information:

    RefSeq Version XM_008767009.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767009.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    GTTCCTGTGA GAACGACATC TCGTTCCCCT GTCCTGTCCC GTCGAGATTC TCCACTGCAG
    GGCAGTGGGC AGCAAAATAG CCAAGCAGGA CAGAGAAATT CCACCAGCAG TATTGAGCCC
    CGGCTTCTAT GGGAGAGAGT GGAAAAGCTG GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA
    GGGTCCAGCA ACTCAGGATC CCAGCCTGGC TCCCATCCTG GGTCTCAGAG TGGCTCTGGT
    GAACGCTTCA GAGTGAGATC ATCATCCAAG TCTGAAGGTT CTCCATCTCA GCGCCTTGAA
    AATGCAGCCA AAAAACCTGA AGATAAGAAA GAAGTATTTC GACCTCTCAA ACCTGCTGGA
    GAAGTGGACT TGACTGCGTT GGCCAAAGAG CTTCGAGCAG TGGAAGATGT GCGGCCACCT
    CACAAAGTGA CAGACTATTC CTCCTCCAGT GAGGAGTCTG GGACCACGGA TGAGGAGGAG
    GAGGATGTGG AACAGGAGGG GGCTGATGAT TCCACCTCGG GGCCAGAGGA CACCAGAGCA
    GCGTCGTCTC TGAATCTGAG CAACGGTGAG ACAGAATCTG TGAAAACAAT GATTGTTCAT
    GATGACGTAG AAAGTGAACC AGCCATGACC CCATCCAAGG AGGGCACCCT AATCGTCCGC
    CAGAGTACAG TTGACCAAAA GCGTGCCAGC CATCATGAGA GCAATGGCTT TGCGGGTCGC
    ATTCACCTCT TGCCAGATCT CTTACAGCAA AGCCATTCCT CCTCCACTTC CTCCACCTCT
    TCCTCCCCAT CCTCCAGCCA GCCGACACCC ACCATGTCCC CACAGACACC CCAGGACAAG
    CTCACTGCTA ATGAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC
    TCCTTTACAC CTTTTATAGA CCCCAGGTTA CTACAGATCT CTCCATCTAG TGGAACAACA
    GTTACTTCCG TTGTGGGATT TTCCTGTGAT GGGCTGAGAC CAGAAGCCAT AAGGCAAGAT
    CCTACTCGGA AAGGCTCAGT GGTCAATGTG AACCCCACGA ACACTAGGCC ACAGAGTGAT
    ACCCCCGAGA TTCGTAAATA CAAGAAGAGA TTTAACTCAG AGATCCTGTG TGCTGCCTTA
    TGGGGAGTGA ATTTGCTAGT TGGCACAGAG AGTGGCCTGA TGCTCTTGGA CAGAAGTGGC
    CAAGGGAAAG TGTACCCTCT GATCAGCCGG AGACGGTTCC AGCAAATGGA TGTACTTGAG
    GGCCTGAATG TCCTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGCGT CTACTATTTA
    TCCTGGTTAA GAAATAAAAT ACTTCATAAT GATCCGGAAG TTGAGAAGAA GCAGGGGTGG
    ACCACCGTGG GCGACCTGGA AGGGTGCGTG CACTATAAAG TCGTAAAATA TGAAAGAATC
    AAGTTTCTGG TAATCGCTTT GAAGAGTTCT GTGGAAGTCT ATGCATGGGC ACCGAAGCCA
    TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAACTGG TACATAAGCC ATTACTGGTG
    GATCTCACTG TCGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC
    CATGCTGTTG ATGTGGATTC CGGCTCAGTC TATGACATTT ATCTACCAAC ACACGTAAGA
    AAGAACCCAC ACTCTATGAT TCAGTGTACC ATCAAACCCC ATGCAATCAT TATCCTCCCC
    AACACGGATG GAATGGAGCT TCTGGTGTGC TACGAAGATG AAGGGGTTTA TGTGAACACG
    TACGGAAGAA TCACCAAGGA CGTGGTTCTG CAGTGGGGAG AGATGCCAAC ATCTGTAGCA
    TACATCCGGT CGAATCAGAC AATGGGCTGG GGAGAGAAGG CAATAGAGAT ACGCTCTGTG
    GAAACTGGTC ATTTGGACGG TGTGTTTATG CACAAAAGGG CTCAGAGACT AAAGTTCCTG
    TGTGAACGTA ATGACAAGGT GTTCTTTGCT TCCGTCCGCT CTGGCGGCAG CAGCCAGGTC
    TACTTCATGA CCTTAGGCAG GACTTCCCTT CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62701
    Clone ID Related Accession (Same CDS sequence) XM_008767010.2
    Accession Version XM_008767010.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTACA GTGGTCCCAC
    CTGGCATCTC TCAAGAACAA TGTCTCCCCT GTCTCGAGAT CCCATTCCTT CAGTGACCCT
    TCTCCCAAAT TCGCACACCA CCATCTCCGC TCTCAGGACC CATGTCCACC TTCCCGCAGT
    GAGGGGCTCA GTCAGAGCTC TGACTCTAAG TCGGAGGTAC CTGAGCCCAC CCAAAAGGCT
    TGGTCTAGAT CAGACAGTGA CGAGGTGCCT CCAAGGGTTC CTGTGAGAAC GACATCTCGT
    TCCCCTGTCC TGTCCCGTCG AGATTCTCCA CTGCAGGGCA GTGGGCAGCA AAATAGCCAA
    GCAGGACAGA GAAATTCCAC CAGCAGTATT GAGCCCCGGC TTCTATGGGA GAGAGTGGAA
    AAGCTGGTCC CCAGGCCAGG CAGTGGCAGC TCTTCAGGGT CCAGCAACTC AGGATCCCAG
    CCTGGCTCCC ATCCTGGGTC TCAGAGTGGC TCTGGTGAAC GCTTCAGAGT GAGATCATCA
    TCCAAGTCTG AAGGTTCTCC ATCTCAGCGC CTTGAAAATG CAGCCAAAAA ACCTGAAGAT
    AAGAAAGAAG TATTTCGACC TCTCAAACCT GCTGGAGAAG TGGACTTGAC TGCGTTGGCC
    AAAGAGCTTC GAGCAGTGGA AGATGTGCGG CCACCTCACA AAGTGACAGA CTATTCCTCC
    TCCAGTGAGG AGTCTGGGAC CACGGATGAG GAGGAGGAGG ATGTGGAACA GGAGGGGGCT
    GATGATTCCA CCTCGGGGCC AGAGGACACC AGAGCAGCGT CGTCTCTGAA TCTGAGCAAC
    GGTGAGACAG AATCTGTGAA AACAATGATT GTTCATGATG ACGTAGAAAG TGAACCAGCC
    ATGACCCCAT CCAAGGAGGG CACCCTAATC GTCCGCCAGA GTACAGTTGA CCAAAAGCGT
    GCCAGCCATC ATGAGAGCAA TGGCTTTGCG GGTCGCATTC ACCTCTTGCC AGATCTCTTA
    CAGCAAAGCC ATTCCTCCTC CACTTCCTCC ACCTCTTCCT CCCCATCCTC CAGCCAGCCG
    ACACCCACCA TGTCCCCACA GACACCCCAG GACAAGCTCA CTGCTAATGA GACTCAGTCC
    GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC
    AGGTTACTAC AGATCTCTCC ATCTAGTGGA ACAACAGTTA CTTCCGTTGT GGGATTTTCC
    TGTGATGGGC TGAGACCAGA AGCCATAAGG CAAGATCCTA CTCGGAAAGG CTCAGTGGTC
    AATGTGAACC CCACGAACAC TAGGCCACAG AGTGATACCC CCGAGATTCG TAAATACAAG
    AAGAGATTTA ACTCAGAGAT CCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTTGGC
    ACAGAGAGTG GCCTGATGCT CTTGGACAGA AGTGGCCAAG GGAAAGTGTA CCCTCTGATC
    AGCCGGAGAC GGTTCCAGCA AATGGATGTA CTTGAGGGCC TGAATGTCCT GGTGACAATA
    TCTGGCAAAA AGGATAAGTT ACGCGTCTAC TATTTATCCT GGTTAAGAAA TAAAATACTT
    CATAATGATC CGGAAGTTGA GAAGAAGCAG GGGTGGACCA CCGTGGGCGA CCTGGAAGGG
    TGCGTGCACT ATAAAGTCGT AAAATATGAA AGAATCAAGT TTCTGGTAAT CGCTTTGAAG
    AGTTCTGTGG AAGTCTATGC ATGGGCACCG AAGCCATATC ACAAATTTAT GGCCTTTAAG
    TCATTTGGAG AACTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTCGA GGAAGGCCAG
    AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCCGGC
    TCAGTCTATG ACATTTATCT ACCAACACAC GTAAGAAAGA ACCCACACTC TATGATTCAG
    TGTACCATCA AACCCCATGC AATCATTATC CTCCCCAACA CGGATGGAAT GGAGCTTCTG
    GTGTGCTACG AAGATGAAGG GGTTTATGTG AACACGTACG GAAGAATCAC CAAGGACGTG
    GTTCTGCAGT GGGGAGAGAT GCCAACATCT GTAGCATACA TCCGGTCGAA TCAGACAATG
    GGCTGGGGAG AGAAGGCAAT AGAGATACGC TCTGTGGAAA CTGGTCATTT GGACGGTGTG
    TTTATGCACA AAAGGGCTCA GAGACTAAAG TTCCTGTGTG AACGTAATGA CAAGGTGTTC
    TTTGCTTCCG TCCGCTCTGG CGGCAGCAGC CAGGTCTACT TCATGACCTT AGGCAGGACT
    TCCCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765232.2
    CDS210..4127
    Translation

    Target ORF information:

    RefSeq Version XM_008767010.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767010.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTACA GTGGTCCCAC
    CTGGCATCTC TCAAGAACAA TGTCTCCCCT GTCTCGAGAT CCCATTCCTT CAGTGACCCT
    TCTCCCAAAT TCGCACACCA CCATCTCCGC TCTCAGGACC CATGTCCACC TTCCCGCAGT
    GAGGGGCTCA GTCAGAGCTC TGACTCTAAG TCGGAGGTAC CTGAGCCCAC CCAAAAGGCT
    TGGTCTAGAT CAGACAGTGA CGAGGTGCCT CCAAGGGTTC CTGTGAGAAC GACATCTCGT
    TCCCCTGTCC TGTCCCGTCG AGATTCTCCA CTGCAGGGCA GTGGGCAGCA AAATAGCCAA
    GCAGGACAGA GAAATTCCAC CAGCAGTATT GAGCCCCGGC TTCTATGGGA GAGAGTGGAA
    AAGCTGGTCC CCAGGCCAGG CAGTGGCAGC TCTTCAGGGT CCAGCAACTC AGGATCCCAG
    CCTGGCTCCC ATCCTGGGTC TCAGAGTGGC TCTGGTGAAC GCTTCAGAGT GAGATCATCA
    TCCAAGTCTG AAGGTTCTCC ATCTCAGCGC CTTGAAAATG CAGCCAAAAA ACCTGAAGAT
    AAGAAAGAAG TATTTCGACC TCTCAAACCT GCTGGAGAAG TGGACTTGAC TGCGTTGGCC
    AAAGAGCTTC GAGCAGTGGA AGATGTGCGG CCACCTCACA AAGTGACAGA CTATTCCTCC
    TCCAGTGAGG AGTCTGGGAC CACGGATGAG GAGGAGGAGG ATGTGGAACA GGAGGGGGCT
    GATGATTCCA CCTCGGGGCC AGAGGACACC AGAGCAGCGT CGTCTCTGAA TCTGAGCAAC
    GGTGAGACAG AATCTGTGAA AACAATGATT GTTCATGATG ACGTAGAAAG TGAACCAGCC
    ATGACCCCAT CCAAGGAGGG CACCCTAATC GTCCGCCAGA GTACAGTTGA CCAAAAGCGT
    GCCAGCCATC ATGAGAGCAA TGGCTTTGCG GGTCGCATTC ACCTCTTGCC AGATCTCTTA
    CAGCAAAGCC ATTCCTCCTC CACTTCCTCC ACCTCTTCCT CCCCATCCTC CAGCCAGCCG
    ACACCCACCA TGTCCCCACA GACACCCCAG GACAAGCTCA CTGCTAATGA GACTCAGTCC
    GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC
    AGGTTACTAC AGATCTCTCC ATCTAGTGGA ACAACAGTTA CTTCCGTTGT GGGATTTTCC
    TGTGATGGGC TGAGACCAGA AGCCATAAGG CAAGATCCTA CTCGGAAAGG CTCAGTGGTC
    AATGTGAACC CCACGAACAC TAGGCCACAG AGTGATACCC CCGAGATTCG TAAATACAAG
    AAGAGATTTA ACTCAGAGAT CCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTTGGC
    ACAGAGAGTG GCCTGATGCT CTTGGACAGA AGTGGCCAAG GGAAAGTGTA CCCTCTGATC
    AGCCGGAGAC GGTTCCAGCA AATGGATGTA CTTGAGGGCC TGAATGTCCT GGTGACAATA
    TCTGGCAAAA AGGATAAGTT ACGCGTCTAC TATTTATCCT GGTTAAGAAA TAAAATACTT
    CATAATGATC CGGAAGTTGA GAAGAAGCAG GGGTGGACCA CCGTGGGCGA CCTGGAAGGG
    TGCGTGCACT ATAAAGTCGT AAAATATGAA AGAATCAAGT TTCTGGTAAT CGCTTTGAAG
    AGTTCTGTGG AAGTCTATGC ATGGGCACCG AAGCCATATC ACAAATTTAT GGCCTTTAAG
    TCATTTGGAG AACTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTCGA GGAAGGCCAG
    AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCCGGC
    TCAGTCTATG ACATTTATCT ACCAACACAC GTAAGAAAGA ACCCACACTC TATGATTCAG
    TGTACCATCA AACCCCATGC AATCATTATC CTCCCCAACA CGGATGGAAT GGAGCTTCTG
    GTGTGCTACG AAGATGAAGG GGTTTATGTG AACACGTACG GAAGAATCAC CAAGGACGTG
    GTTCTGCAGT GGGGAGAGAT GCCAACATCT GTAGCATACA TCCGGTCGAA TCAGACAATG
    GGCTGGGGAG AGAAGGCAAT AGAGATACGC TCTGTGGAAA CTGGTCATTT GGACGGTGTG
    TTTATGCACA AAAGGGCTCA GAGACTAAAG TTCCTGTGTG AACGTAATGA CAAGGTGTTC
    TTTGCTTCCG TCCGCTCTGG CGGCAGCAGC CAGGTCTACT TCATGACCTT AGGCAGGACT
    TCCCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62702
    Clone ID Related Accession (Same CDS sequence) XM_017596341.1
    Accession Version XM_017596341.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT
    AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG
    CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT
    GCCCTGCAGA GACCAGCGGA GCCACAGGTT CCTGTGAGAA CGACATCTCG TTCCCCTGTC
    CTGTCCCGTC GAGATTCTCC ACTGCAGGGC AGTGGGCAGC AAAATAGCCA AGCAGGACAG
    AGAAATTCCA CCAGCAGTAT TGAGCCCCGG CTTCTATGGG AGAGAGTGGA AAAGCTGGTC
    CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG TCCAGCAACT CAGGATCCCA GCCTGGCTCC
    CATCCTGGGT CTCAGAGTGG CTCTGGTGAA CGCTTCAGAG TGAGATCATC ATCCAAGTCT
    GAAGGTTCTC CATCTCAGCG CCTTGAAAAT GCAGCCAAAA AACCTGAAGA TAAGAAAGAA
    GTATTTCGAC CTCTCAAACC TGCTGGAGAA GTGGACTTGA CTGCGTTGGC CAAAGAGCTT
    CGAGCAGTGG AAGATGTGCG GCCACCTCAC AAAGTGACAG ACTATTCCTC CTCCAGTGAG
    GAGTCTGGGA CCACGGATGA GGAGGAGGAG GATGTGGAAC AGGAGGGGGC TGATGATTCC
    ACCTCGGGGC CAGAGGACAC CAGAGCAGCG TCGTCTCTGA ATCTGAGCAA CGGTGAGACA
    GAATCTGTGA AAACAATGAT TGTTCATGAT GACGTAGAAA GTGAACCAGC CATGACCCCA
    TCCAAGGAGG GCACCCTAAT CGTCCGCCAG AGTACAGTTG ACCAAAAGCG TGCCAGCCAT
    CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC
    CATTCCTCCT CCACTTCCTC CACCTCTTCC TCCCCATCCT CCAGCCAGCC GACACCCACC
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTGCTAATG AGACTCAGTC CGCTAGTAGC
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGGTTACTA
    CAGATCTCTC CATCTAGTGG AACAACAGTT ACTTCCGTTG TGGGATTTTC CTGTGATGGG
    CTGAGACCAG AAGCCATAAG GCAAGATCCT ACTCGGAAAG GCTCAGTGGT CAATGTGAAC
    CCCACGAACA CTAGGCCACA GAGTGATACC CCCGAGATTC GTAAATACAA GAAGAGATTT
    AACTCAGAGA TCCTGTGTGC TGCCTTATGG GGAGTGAATT TGCTAGTTGG CACAGAGAGT
    GGCCTGATGC TCTTGGACAG AAGTGGCCAA GGGAAAGTGT ACCCTCTGAT CAGCCGGAGA
    CGGTTCCAGC AAATGGATGT ACTTGAGGGC CTGAATGTCC TGGTGACAAT ATCTGGCAAA
    AAGGATAAGT TACGCGTCTA CTATTTATCC TGGTTAAGAA ATAAAATACT TCATAATGAT
    CCGGAAGTTG AGAAGAAGCA GGGGTGGACC ACCGTGGGCG ACCTGGAAGG GTGCGTGCAC
    TATAAAGTCG TAAAATATGA AAGAATCAAG TTTCTGGTAA TCGCTTTGAA GAGTTCTGTG
    GAAGTCTATG CATGGGCACC GAAGCCATAT CACAAATTTA TGGCCTTTAA GTCATTTGGA
    GAACTGGTAC ATAAGCCATT ACTGGTGGAT CTCACTGTCG AGGAAGGCCA GAGGTTGAAA
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGATTCCGG CTCAGTCTAT
    GACATTTATC TACCAACACA CGTAAGAAAG AACCCACACT CTATGATTCA GTGTACCATC
    AAACCCCATG CAATCATTAT CCTCCCCAAC ACGGATGGAA TGGAGCTTCT GGTGTGCTAC
    GAAGATGAAG GGGTTTATGT GAACACGTAC GGAAGAATCA CCAAGGACGT GGTTCTGCAG
    TGGGGAGAGA TGCCAACATC TGTAGCATAC ATCCGGTCGA ATCAGACAAT GGGCTGGGGA
    GAGAAGGCAA TAGAGATACG CTCTGTGGAA ACTGGTCATT TGGACGGTGT GTTTATGCAC
    AAAAGGGCTC AGAGACTAAA GTTCCTGTGT GAACGTAATG ACAAGGTGTT CTTTGCTTCC
    GTCCGCTCTG GCGGCAGCAG CCAGGTCTAC TTCATGACCT TAGGCAGGAC TTCCCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017451830.1
    CDS210..4058
    Translation

    Target ORF information:

    RefSeq Version XM_017596341.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017596341.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT
    AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG
    CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT
    GCCCTGCAGA GACCAGCGGA GCCACAGGTT CCTGTGAGAA CGACATCTCG TTCCCCTGTC
    CTGTCCCGTC GAGATTCTCC ACTGCAGGGC AGTGGGCAGC AAAATAGCCA AGCAGGACAG
    AGAAATTCCA CCAGCAGTAT TGAGCCCCGG CTTCTATGGG AGAGAGTGGA AAAGCTGGTC
    CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG TCCAGCAACT CAGGATCCCA GCCTGGCTCC
    CATCCTGGGT CTCAGAGTGG CTCTGGTGAA CGCTTCAGAG TGAGATCATC ATCCAAGTCT
    GAAGGTTCTC CATCTCAGCG CCTTGAAAAT GCAGCCAAAA AACCTGAAGA TAAGAAAGAA
    GTATTTCGAC CTCTCAAACC TGCTGGAGAA GTGGACTTGA CTGCGTTGGC CAAAGAGCTT
    CGAGCAGTGG AAGATGTGCG GCCACCTCAC AAAGTGACAG ACTATTCCTC CTCCAGTGAG
    GAGTCTGGGA CCACGGATGA GGAGGAGGAG GATGTGGAAC AGGAGGGGGC TGATGATTCC
    ACCTCGGGGC CAGAGGACAC CAGAGCAGCG TCGTCTCTGA ATCTGAGCAA CGGTGAGACA
    GAATCTGTGA AAACAATGAT TGTTCATGAT GACGTAGAAA GTGAACCAGC CATGACCCCA
    TCCAAGGAGG GCACCCTAAT CGTCCGCCAG AGTACAGTTG ACCAAAAGCG TGCCAGCCAT
    CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC
    CATTCCTCCT CCACTTCCTC CACCTCTTCC TCCCCATCCT CCAGCCAGCC GACACCCACC
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTGCTAATG AGACTCAGTC CGCTAGTAGC
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGGTTACTA
    CAGATCTCTC CATCTAGTGG AACAACAGTT ACTTCCGTTG TGGGATTTTC CTGTGATGGG
    CTGAGACCAG AAGCCATAAG GCAAGATCCT ACTCGGAAAG GCTCAGTGGT CAATGTGAAC
    CCCACGAACA CTAGGCCACA GAGTGATACC CCCGAGATTC GTAAATACAA GAAGAGATTT
    AACTCAGAGA TCCTGTGTGC TGCCTTATGG GGAGTGAATT TGCTAGTTGG CACAGAGAGT
    GGCCTGATGC TCTTGGACAG AAGTGGCCAA GGGAAAGTGT ACCCTCTGAT CAGCCGGAGA
    CGGTTCCAGC AAATGGATGT ACTTGAGGGC CTGAATGTCC TGGTGACAAT ATCTGGCAAA
    AAGGATAAGT TACGCGTCTA CTATTTATCC TGGTTAAGAA ATAAAATACT TCATAATGAT
    CCGGAAGTTG AGAAGAAGCA GGGGTGGACC ACCGTGGGCG ACCTGGAAGG GTGCGTGCAC
    TATAAAGTCG TAAAATATGA AAGAATCAAG TTTCTGGTAA TCGCTTTGAA GAGTTCTGTG
    GAAGTCTATG CATGGGCACC GAAGCCATAT CACAAATTTA TGGCCTTTAA GTCATTTGGA
    GAACTGGTAC ATAAGCCATT ACTGGTGGAT CTCACTGTCG AGGAAGGCCA GAGGTTGAAA
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGATTCCGG CTCAGTCTAT
    GACATTTATC TACCAACACA CGTAAGAAAG AACCCACACT CTATGATTCA GTGTACCATC
    AAACCCCATG CAATCATTAT CCTCCCCAAC ACGGATGGAA TGGAGCTTCT GGTGTGCTAC
    GAAGATGAAG GGGTTTATGT GAACACGTAC GGAAGAATCA CCAAGGACGT GGTTCTGCAG
    TGGGGAGAGA TGCCAACATC TGTAGCATAC ATCCGGTCGA ATCAGACAAT GGGCTGGGGA
    GAGAAGGCAA TAGAGATACG CTCTGTGGAA ACTGGTCATT TGGACGGTGT GTTTATGCAC
    AAAAGGGCTC AGAGACTAAA GTTCCTGTGT GAACGTAATG ACAAGGTGTT CTTTGCTTCC
    GTCCGCTCTG GCGGCAGCAG CCAGGTCTAC TTCATGACCT TAGGCAGGAC TTCCCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62703
    Clone ID Related Accession (Same CDS sequence) XM_008767011.2
    Accession Version XM_008767011.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT
    AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG
    CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT
    GCCCTGCAGA GACCAGCGGA GCCACAGGTT CCTGTGAGAA CGACATCTCG TTCCCCTGTC
    CTGTCCCGTC GAGATTCTCC ACTGCAGGGC AGTGGGCAGC AAAATAGCCA AGCAGGACAG
    AGAAATTCCA CCAGCAGTAT TGAGCCCCGG CTTCTATGGG AGAGAGTGGA AAAGCTGGTC
    CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG TCCAGCAACT CAGGATCCCA GCCTGGCTCC
    CATCCTGGGT CTCAGAGTGG CTCTGGTGAA CGCTTCAGAG TGAGATCATC ATCCAAGTCT
    GAAGGTTCTC CATCTCAGCG CCTTGAAAAT GCAGCCAAAA AACCTGAAGA TAAGAAAGAA
    GTATTTCGAC CTCTCAAACC TGCTGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG
    GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG
    ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG
    CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG
    AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG
    GGCACCCTAA TCGTCCGCCA GAGTACAGTT GACCAAAAGC GTGCCAGCCA TCATGAGAGC
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC
    TCCACTTCCT CCACCTCTTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA
    CAGACACCCC AGGACAAGCT CACTGCTAAT GAGACTCAGT CCGCTAGTAG CACACTCCAG
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGGTTACT ACAGATCTCT
    CCATCTAGTG GAACAACAGT TACTTCCGTT GTGGGATTTT CCTGTGATGG GCTGAGACCA
    GAAGCCATAA GGCAAGATCC TACTCGGAAA GGCTCAGTGG TCAATGTGAA CCCCACGAAC
    ACTAGGCCAC AGAGTGATAC CCCCGAGATT CGTAAATACA AGAAGAGATT TAACTCAGAG
    ATCCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTTG GCACAGAGAG TGGCCTGATG
    CTCTTGGACA GAAGTGGCCA AGGGAAAGTG TACCCTCTGA TCAGCCGGAG ACGGTTCCAG
    CAAATGGATG TACTTGAGGG CCTGAATGTC CTGGTGACAA TATCTGGCAA AAAGGATAAG
    TTACGCGTCT ACTATTTATC CTGGTTAAGA AATAAAATAC TTCATAATGA TCCGGAAGTT
    GAGAAGAAGC AGGGGTGGAC CACCGTGGGC GACCTGGAAG GGTGCGTGCA CTATAAAGTC
    GTAAAATATG AAAGAATCAA GTTTCTGGTA ATCGCTTTGA AGAGTTCTGT GGAAGTCTAT
    GCATGGGCAC CGAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAACTGGTA
    CATAAGCCAT TACTGGTGGA TCTCACTGTC GAGGAAGGCC AGAGGTTGAA AGTGATCTAT
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCCG GCTCAGTCTA TGACATTTAT
    CTACCAACAC ACATTCAGTG TACCATCAAA CCCCATGCAA TCATTATCCT CCCCAACACG
    GATGGAATGG AGCTTCTGGT GTGCTACGAA GATGAAGGGG TTTATGTGAA CACGTACGGA
    AGAATCACCA AGGACGTGGT TCTGCAGTGG GGAGAGATGC CAACATCTGT AGCATACATC
    CGGTCGAATC AGACAATGGG CTGGGGAGAG AAGGCAATAG AGATACGCTC TGTGGAAACT
    GGTCATTTGG ACGGTGTGTT TATGCACAAA AGGGCTCAGA GACTAAAGTT CCTGTGTGAA
    CGTAATGACA AGGTGTTCTT TGCTTCCGTC CGCTCTGGCG GCAGCAGCCA GGTCTACTTC
    ATGACCTTAG GCAGGACTTC CCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765233.2
    CDS208..4023
    Translation

    Target ORF information:

    RefSeq Version XM_008767011.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767011.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT
    AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG
    CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT
    GCCCTGCAGA GACCAGCGGA GCCACAGGTT CCTGTGAGAA CGACATCTCG TTCCCCTGTC
    CTGTCCCGTC GAGATTCTCC ACTGCAGGGC AGTGGGCAGC AAAATAGCCA AGCAGGACAG
    AGAAATTCCA CCAGCAGTAT TGAGCCCCGG CTTCTATGGG AGAGAGTGGA AAAGCTGGTC
    CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG TCCAGCAACT CAGGATCCCA GCCTGGCTCC
    CATCCTGGGT CTCAGAGTGG CTCTGGTGAA CGCTTCAGAG TGAGATCATC ATCCAAGTCT
    GAAGGTTCTC CATCTCAGCG CCTTGAAAAT GCAGCCAAAA AACCTGAAGA TAAGAAAGAA
    GTATTTCGAC CTCTCAAACC TGCTGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG
    GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG
    ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG
    CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG
    AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG
    GGCACCCTAA TCGTCCGCCA GAGTACAGTT GACCAAAAGC GTGCCAGCCA TCATGAGAGC
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC
    TCCACTTCCT CCACCTCTTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA
    CAGACACCCC AGGACAAGCT CACTGCTAAT GAGACTCAGT CCGCTAGTAG CACACTCCAG
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGGTTACT ACAGATCTCT
    CCATCTAGTG GAACAACAGT TACTTCCGTT GTGGGATTTT CCTGTGATGG GCTGAGACCA
    GAAGCCATAA GGCAAGATCC TACTCGGAAA GGCTCAGTGG TCAATGTGAA CCCCACGAAC
    ACTAGGCCAC AGAGTGATAC CCCCGAGATT CGTAAATACA AGAAGAGATT TAACTCAGAG
    ATCCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTTG GCACAGAGAG TGGCCTGATG
    CTCTTGGACA GAAGTGGCCA AGGGAAAGTG TACCCTCTGA TCAGCCGGAG ACGGTTCCAG
    CAAATGGATG TACTTGAGGG CCTGAATGTC CTGGTGACAA TATCTGGCAA AAAGGATAAG
    TTACGCGTCT ACTATTTATC CTGGTTAAGA AATAAAATAC TTCATAATGA TCCGGAAGTT
    GAGAAGAAGC AGGGGTGGAC CACCGTGGGC GACCTGGAAG GGTGCGTGCA CTATAAAGTC
    GTAAAATATG AAAGAATCAA GTTTCTGGTA ATCGCTTTGA AGAGTTCTGT GGAAGTCTAT
    GCATGGGCAC CGAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAACTGGTA
    CATAAGCCAT TACTGGTGGA TCTCACTGTC GAGGAAGGCC AGAGGTTGAA AGTGATCTAT
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCCG GCTCAGTCTA TGACATTTAT
    CTACCAACAC ACATTCAGTG TACCATCAAA CCCCATGCAA TCATTATCCT CCCCAACACG
    GATGGAATGG AGCTTCTGGT GTGCTACGAA GATGAAGGGG TTTATGTGAA CACGTACGGA
    AGAATCACCA AGGACGTGGT TCTGCAGTGG GGAGAGATGC CAACATCTGT AGCATACATC
    CGGTCGAATC AGACAATGGG CTGGGGAGAG AAGGCAATAG AGATACGCTC TGTGGAAACT
    GGTCATTTGG ACGGTGTGTT TATGCACAAA AGGGCTCAGA GACTAAAGTT CCTGTGTGAA
    CGTAATGACA AGGTGTTCTT TGCTTCCGTC CGCTCTGGCG GCAGCAGCCA GGTCTACTTC
    ATGACCTTAG GCAGGACTTC CCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62704
    Clone ID Related Accession (Same CDS sequence) XM_008767012.2
    Accession Version XM_008767012.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767012.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT TCCTGTGAGA ACGACATCTC GTTCCCCTGT CCTGTCCCGT
    CGAGATTCTC CACTGCAGGG CAGTGGGCAG CAAAATAGCC AAGCAGGACA GAGAAATTCC
    ACCAGCAGTA TTGAGCCCCG GCTTCTATGG GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA
    GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC TCAGGATCCC AGCCTGGCTC CCATCCTGGG
    TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA GTGAGATCAT CATCCAAGTC TGAAGGTTCT
    CCATCTCAGC GCCTTGAAAA TGCAGCCAAA AAACCTGAAG ATAAGAAAGA AGTATTTCGA
    CCTCTCAAAC CTGCTGGAGA AGTGGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG
    GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG
    ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG
    CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG
    AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG
    GGCACCCTAA TCGTCCGCCA GAGTACAGTT GACCAAAAGC GTGCCAGCCA TCATGAGAGC
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC
    TCCACTTCCT CCACCTCTTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA
    CAGACACCCC AGGACAAGCT CACTGCTAAT GAGACTCAGT CCGCTAGTAG CACACTCCAG
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGGTTACT ACAGATCTCT
    CCATCTAGTG GAACAACAGT TACTTCCGTT GTGGGATTTT CCTGTGATGG GCTGAGACCA
    GAAGCCATAA GGCAAGATCC TACTCGGAAA GGCTCAGTGG TCAATGTGAA CCCCACGAAC
    ACTAGGCCAC AGAGTGATAC CCCCGAGATT CGTAAATACA AGAAGAGATT TAACTCAGAG
    ATCCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTTG GCACAGAGAG TGGCCTGATG
    CTCTTGGACA GAAGTGGCCA AGGGAAAGTG TACCCTCTGA TCAGCCGGAG ACGGTTCCAG
    CAAATGGATG TACTTGAGGG CCTGAATGTC CTGGTGACAA TATCTGGCAA AAAGGATAAG
    TTACGCGTCT ACTATTTATC CTGGTTAAGA AATAAAATAC TTCATAATGA TCCGGAAGTT
    GAGAAGAAGC AGGGGTGGAC CACCGTGGGC GACCTGGAAG GGTGCGTGCA CTATAAAGTC
    GTAAAATATG AAAGAATCAA GTTTCTGGTA ATCGCTTTGA AGAGTTCTGT GGAAGTCTAT
    GCATGGGCAC CGAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAACTGGTA
    CATAAGCCAT TACTGGTGGA TCTCACTGTC GAGGAAGGCC AGAGGTTGAA AGTGATCTAT
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCCG GCTCAGTCTA TGACATTTAT
    CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATTC AGTGTACCAT CAAACCCCAT
    GCAATCATTA TCCTCCCCAA CACGGATGGA ATGGAGCTTC TGGTGTGCTA CGAAGATGAA
    GGGGTTTATG TGAACACGTA CGGAAGAATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG
    ATGCCAACAT CTGTAGCATA CATCCGGTCG AATCAGACAA TGGGCTGGGG AGAGAAGGCA
    ATAGAGATAC GCTCTGTGGA AACTGGTCAT TTGGACGGTG TGTTTATGCA CAAAAGGGCT
    CAGAGACTAA AGTTCCTGTG TGAACGTAAT GACAAGGTGT TCTTTGCTTC CGTCCGCTCT
    GGCGGCAGCA GCCAGGTCTA CTTCATGACC TTAGGCAGGA CTTCCCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765234.2
    CDS209..3988
    Translation

    Target ORF information:

    RefSeq Version XM_008767012.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767012.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT TCCTGTGAGA ACGACATCTC GTTCCCCTGT CCTGTCCCGT
    CGAGATTCTC CACTGCAGGG CAGTGGGCAG CAAAATAGCC AAGCAGGACA GAGAAATTCC
    ACCAGCAGTA TTGAGCCCCG GCTTCTATGG GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA
    GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC TCAGGATCCC AGCCTGGCTC CCATCCTGGG
    TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA GTGAGATCAT CATCCAAGTC TGAAGGTTCT
    CCATCTCAGC GCCTTGAAAA TGCAGCCAAA AAACCTGAAG ATAAGAAAGA AGTATTTCGA
    CCTCTCAAAC CTGCTGGAGA AGTGGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG
    GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG
    ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG
    CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG
    AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG
    GGCACCCTAA TCGTCCGCCA GAGTACAGTT GACCAAAAGC GTGCCAGCCA TCATGAGAGC
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC
    TCCACTTCCT CCACCTCTTC CTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA
    CAGACACCCC AGGACAAGCT CACTGCTAAT GAGACTCAGT CCGCTAGTAG CACACTCCAG
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGGTTACT ACAGATCTCT
    CCATCTAGTG GAACAACAGT TACTTCCGTT GTGGGATTTT CCTGTGATGG GCTGAGACCA
    GAAGCCATAA GGCAAGATCC TACTCGGAAA GGCTCAGTGG TCAATGTGAA CCCCACGAAC
    ACTAGGCCAC AGAGTGATAC CCCCGAGATT CGTAAATACA AGAAGAGATT TAACTCAGAG
    ATCCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTTG GCACAGAGAG TGGCCTGATG
    CTCTTGGACA GAAGTGGCCA AGGGAAAGTG TACCCTCTGA TCAGCCGGAG ACGGTTCCAG
    CAAATGGATG TACTTGAGGG CCTGAATGTC CTGGTGACAA TATCTGGCAA AAAGGATAAG
    TTACGCGTCT ACTATTTATC CTGGTTAAGA AATAAAATAC TTCATAATGA TCCGGAAGTT
    GAGAAGAAGC AGGGGTGGAC CACCGTGGGC GACCTGGAAG GGTGCGTGCA CTATAAAGTC
    GTAAAATATG AAAGAATCAA GTTTCTGGTA ATCGCTTTGA AGAGTTCTGT GGAAGTCTAT
    GCATGGGCAC CGAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAACTGGTA
    CATAAGCCAT TACTGGTGGA TCTCACTGTC GAGGAAGGCC AGAGGTTGAA AGTGATCTAT
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCCG GCTCAGTCTA TGACATTTAT
    CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATTC AGTGTACCAT CAAACCCCAT
    GCAATCATTA TCCTCCCCAA CACGGATGGA ATGGAGCTTC TGGTGTGCTA CGAAGATGAA
    GGGGTTTATG TGAACACGTA CGGAAGAATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG
    ATGCCAACAT CTGTAGCATA CATCCGGTCG AATCAGACAA TGGGCTGGGG AGAGAAGGCA
    ATAGAGATAC GCTCTGTGGA AACTGGTCAT TTGGACGGTG TGTTTATGCA CAAAAGGGCT
    CAGAGACTAA AGTTCCTGTG TGAACGTAAT GACAAGGTGT TCTTTGCTTC CGTCCGCTCT
    GGCGGCAGCA GCCAGGTCTA CTTCATGACC TTAGGCAGGA CTTCCCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62705
    Clone ID Related Accession (Same CDS sequence) XM_017596342.1
    Accession Version XM_017596342.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3747bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT TCCTGTGAGA ACGACATCTC GTTCCCCTGT CCTGTCCCGT
    CGAGATTCTC CACTGCAGGG CAGTGGGCAG CAAAATAGCC AAGCAGGACA GAGAAATTCC
    ACCAGCAGTA TTGAGCCCCG GCTTCTATGG GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA
    GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC TCAGGATCCC AGCCTGGCTC CCATCCTGGG
    TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA GTGAGATCAT CATCCAAGTC TGAAGGTTCT
    CCATCTCAGC GCCTTGAAAA TGCAGCCAAA AAACCTGAAG ATAAGAAAGA AGTATTTCGA
    CCTCTCAAAC CTGCTGACTT GACTGCGTTG GCCAAAGAGC TTCGAGCAGT GGAAGATGTG
    CGGCCACCTC ACAAAGTGAC AGACTATTCC TCCTCCAGTG AGGAGTCTGG GACCACGGAT
    GAGGAGGAGG AGGATGTGGA ACAGGAGGGG GCTGATGATT CCACCTCGGG GCCAGAGGAC
    ACCAGAGCAG CGTCGTCTCT GAATCTGAGC AACGGTGAGA CAGAATCTGT GAAAACAATG
    ATTGTTCATG ATGACGTAGA AAGTGAACCA GCCATGACCC CATCCAAGGA GGGCACCCTA
    ATCGTCCGCC AGAGTACAGT TGACCAAAAG CGTGCCAGCC ATCATGAGAG CAATGGCTTT
    GCGGGTCGCA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACTTCC
    TCCACCTCTT CCTCCCCATC CTCCAGCCAG CCGACACCCA CCATGTCCCC ACAGACACCC
    CAGGACAAGC TCACTGCTAA TGAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGGTTAC TACAGATCTC TCCATCTAGT
    GGAACAACAG TTACTTCCGT TGTGGGATTT TCCTGTGATG GGCTGAGACC AGAAGCCATA
    AGGCAAGATC CTACTCGGAA AGGCTCAGTG GTCAATGTGA ACCCCACGAA CACTAGGCCA
    CAGAGTGATA CCCCCGAGAT TCGTAAATAC AAGAAGAGAT TTAACTCAGA GATCCTGTGT
    GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT GGCACAGAGA GTGGCCTGAT GCTCTTGGAC
    AGAAGTGGCC AAGGGAAAGT GTACCCTCTG ATCAGCCGGA GACGGTTCCA GCAAATGGAT
    GTACTTGAGG GCCTGAATGT CCTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGCGTC
    TACTATTTAT CCTGGTTAAG AAATAAAATA CTTCATAATG ATCCGGAAGT TGAGAAGAAG
    CAGGGGTGGA CCACCGTGGG CGACCTGGAA GGGTGCGTGC ACTATAAAGT CGTAAAATAT
    GAAAGAATCA AGTTTCTGGT AATCGCTTTG AAGAGTTCTG TGGAAGTCTA TGCATGGGCA
    CCGAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAACTGGT ACATAAGCCA
    TTACTGGTGG ATCTCACTGT CGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT
    GCTGGATTCC ATGCTGTTGA TGTGGATTCC GGCTCAGTCT ATGACATTTA TCTACCAACA
    CACATTCAGT GTACCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGAATG
    GAGCTTCTGG TGTGCTACGA AGATGAAGGG GTTTATGTGA ACACGTACGG AAGAATCACC
    AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCAACATCTG TAGCATACAT CCGGTCGAAT
    CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGCT CTGTGGAAAC TGGTCATTTG
    GACGGTGTGT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGTAATGAC
    AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC GGCAGCAGCC AGGTCTACTT CATGACCTTA
    GGCAGGACTT CCCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017451831.1
    CDS207..3953
    Translation

    Target ORF information:

    RefSeq Version XM_017596342.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017596342.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT TCCTGTGAGA ACGACATCTC GTTCCCCTGT CCTGTCCCGT
    CGAGATTCTC CACTGCAGGG CAGTGGGCAG CAAAATAGCC AAGCAGGACA GAGAAATTCC
    ACCAGCAGTA TTGAGCCCCG GCTTCTATGG GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA
    GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC TCAGGATCCC AGCCTGGCTC CCATCCTGGG
    TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA GTGAGATCAT CATCCAAGTC TGAAGGTTCT
    CCATCTCAGC GCCTTGAAAA TGCAGCCAAA AAACCTGAAG ATAAGAAAGA AGTATTTCGA
    CCTCTCAAAC CTGCTGACTT GACTGCGTTG GCCAAAGAGC TTCGAGCAGT GGAAGATGTG
    CGGCCACCTC ACAAAGTGAC AGACTATTCC TCCTCCAGTG AGGAGTCTGG GACCACGGAT
    GAGGAGGAGG AGGATGTGGA ACAGGAGGGG GCTGATGATT CCACCTCGGG GCCAGAGGAC
    ACCAGAGCAG CGTCGTCTCT GAATCTGAGC AACGGTGAGA CAGAATCTGT GAAAACAATG
    ATTGTTCATG ATGACGTAGA AAGTGAACCA GCCATGACCC CATCCAAGGA GGGCACCCTA
    ATCGTCCGCC AGAGTACAGT TGACCAAAAG CGTGCCAGCC ATCATGAGAG CAATGGCTTT
    GCGGGTCGCA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACTTCC
    TCCACCTCTT CCTCCCCATC CTCCAGCCAG CCGACACCCA CCATGTCCCC ACAGACACCC
    CAGGACAAGC TCACTGCTAA TGAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGGTTAC TACAGATCTC TCCATCTAGT
    GGAACAACAG TTACTTCCGT TGTGGGATTT TCCTGTGATG GGCTGAGACC AGAAGCCATA
    AGGCAAGATC CTACTCGGAA AGGCTCAGTG GTCAATGTGA ACCCCACGAA CACTAGGCCA
    CAGAGTGATA CCCCCGAGAT TCGTAAATAC AAGAAGAGAT TTAACTCAGA GATCCTGTGT
    GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT GGCACAGAGA GTGGCCTGAT GCTCTTGGAC
    AGAAGTGGCC AAGGGAAAGT GTACCCTCTG ATCAGCCGGA GACGGTTCCA GCAAATGGAT
    GTACTTGAGG GCCTGAATGT CCTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGCGTC
    TACTATTTAT CCTGGTTAAG AAATAAAATA CTTCATAATG ATCCGGAAGT TGAGAAGAAG
    CAGGGGTGGA CCACCGTGGG CGACCTGGAA GGGTGCGTGC ACTATAAAGT CGTAAAATAT
    GAAAGAATCA AGTTTCTGGT AATCGCTTTG AAGAGTTCTG TGGAAGTCTA TGCATGGGCA
    CCGAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAACTGGT ACATAAGCCA
    TTACTGGTGG ATCTCACTGT CGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT
    GCTGGATTCC ATGCTGTTGA TGTGGATTCC GGCTCAGTCT ATGACATTTA TCTACCAACA
    CACATTCAGT GTACCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGAATG
    GAGCTTCTGG TGTGCTACGA AGATGAAGGG GTTTATGTGA ACACGTACGG AAGAATCACC
    AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCAACATCTG TAGCATACAT CCGGTCGAAT
    CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGCT CTGTGGAAAC TGGTCATTTG
    GACGGTGTGT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGTAATGAC
    AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC GGCAGCAGCC AGGTCTACTT CATGACCTTA
    GGCAGGACTT CCCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62706
    Clone ID Related Accession (Same CDS sequence) XM_017596343.1
    Accession Version XM_017596343.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    GTTCCTGTGA GAACGACATC TCGTTCCCCT GTCCTGTCCC GTCGAGATTC TCCACTGCAG
    GGCAGTGGGC AGCAAAATAG CCAAGCAGGA CAGAGAAATT CCACCAGTAT TGAGCCCCGG
    CTTCTATGGG AGAGAGTGGA AAAGCTGGTC CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG
    TCCAGCAACT CAGGATCCCA GCCTGGCTCC CATCCTGGGT CTCAGAGTGG CTCTGGTGAA
    CGCTTCAGAG TGAGATCATC ATCCAAGTCT GAAGGTTCTC CATCTCAGCG CCTTGAAAAT
    GCAGCCAAAA AACCTGAAGA TAAGAAAGAA GTATTTCGAC CTCTCAAACC TGCTGACTTG
    ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTGC GGCCACCTCA CAAAGTGACA
    GACTATTCCT CCTCCAGTGA GGAGTCTGGG ACCACGGATG AGGAGGAGGA GGATGTGGAA
    CAGGAGGGGG CTGATGATTC CACCTCGGGG CCAGAGGACA CCAGAGCAGC GTCGTCTCTG
    AATCTGAGCA ACGGTGAGAC AGAATCTGTG AAAACAATGA TTGTTCATGA TGACGTAGAA
    AGTGAACCAG CCATGACCCC ATCCAAGGAG GGCACCCTAA TCGTCCGCCA GACTCAGTCC
    GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC
    AGGTTACTAC AGATCTCTCC ATCTAGTGGA ACAACAGTTA CTTCCGTTGT GGGATTTTCC
    TGTGATGGGC TGAGACCAGA AGCCATAAGG CAAGATCCTA CTCGGAAAGG CTCAGTGGTC
    AATGTGAACC CCACGAACAC TAGGCCACAG AGTGATACCC CCGAGATTCG TAAATACAAG
    AAGAGATTTA ACTCAGAGAT CCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTTGGC
    ACAGAGAGTG GCCTGATGCT CTTGGACAGA AGTGGCCAAG GGAAAGTGTA CCCTCTGATC
    AGCCGGAGAC GGTTCCAGCA AATGGATGTA CTTGAGGGCC TGAATGTCCT GGTGACAATA
    TCTGGCAAAA AGGATAAGTT ACGCGTCTAC TATTTATCCT GGTTAAGAAA TAAAATACTT
    CATAATGATC CGGAAGTTGA GAAGAAGCAG GGGTGGACCA CCGTGGGCGA CCTGGAAGGG
    TGCGTGCACT ATAAAGTCGT AAAATATGAA AGAATCAAGT TTCTGGTAAT CGCTTTGAAG
    AGTTCTGTGG AAGTCTATGC ATGGGCACCG AAGCCATATC ACAAATTTAT GGCCTTTAAG
    TCATTTGGAG AACTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTCGA GGAAGGCCAG
    AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCCGGC
    TCAGTCTATG ACATTTATCT ACCAACACAC ATTCAGTGTA CCATCAAACC CCATGCAATC
    ATTATCCTCC CCAACACGGA TGGAATGGAG CTTCTGGTGT GCTACGAAGA TGAAGGGGTT
    TATGTGAACA CGTACGGAAG AATCACCAAG GACGTGGTTC TGCAGTGGGG AGAGATGCCA
    ACATCTGTAG CATACATCCG GTCGAATCAG ACAATGGGCT GGGGAGAGAA GGCAATAGAG
    ATACGCTCTG TGGAAACTGG TCATTTGGAC GGTGTGTTTA TGCACAAAAG GGCTCAGAGA
    CTAAAGTTCC TGTGTGAACG TAATGACAAG GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC
    AGCAGCCAGG TCTACTTCAT GACCTTAGGC AGGACTTCCC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017451832.1
    CDS209..3922
    Translation

    Target ORF information:

    RefSeq Version XM_017596343.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017596343.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGGAGTGTCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCACACGCAC AACAGCCGCC ACCCCCGCAG
    CAGCAGGACA GGAGCAAACC CAGCTATCAC GCTCCAGAGC CCAAGCCTCA CTACGACCCT
    GCTGACAGAG CTCGGGAGGT GGAAGATAGA TTTAGAAAGA CTAACCACAG CTCCCCTGAG
    GCCCAGGCTA AGCAGACAGG CAGAGGACTG GAGCCACCCG TGCCTTCCCG GTCAGAATCT
    TTTTCCAATG GCAACTCAGA GTCTGTGCAC CCTGCCCTGC AGAGACCAGC GGAGCCACAG
    GTTCCTGTGA GAACGACATC TCGTTCCCCT GTCCTGTCCC GTCGAGATTC TCCACTGCAG
    GGCAGTGGGC AGCAAAATAG CCAAGCAGGA CAGAGAAATT CCACCAGTAT TGAGCCCCGG
    CTTCTATGGG AGAGAGTGGA AAAGCTGGTC CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG
    TCCAGCAACT CAGGATCCCA GCCTGGCTCC CATCCTGGGT CTCAGAGTGG CTCTGGTGAA
    CGCTTCAGAG TGAGATCATC ATCCAAGTCT GAAGGTTCTC CATCTCAGCG CCTTGAAAAT
    GCAGCCAAAA AACCTGAAGA TAAGAAAGAA GTATTTCGAC CTCTCAAACC TGCTGACTTG
    ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTGC GGCCACCTCA CAAAGTGACA
    GACTATTCCT CCTCCAGTGA GGAGTCTGGG ACCACGGATG AGGAGGAGGA GGATGTGGAA
    CAGGAGGGGG CTGATGATTC CACCTCGGGG CCAGAGGACA CCAGAGCAGC GTCGTCTCTG
    AATCTGAGCA ACGGTGAGAC AGAATCTGTG AAAACAATGA TTGTTCATGA TGACGTAGAA
    AGTGAACCAG CCATGACCCC ATCCAAGGAG GGCACCCTAA TCGTCCGCCA GACTCAGTCC
    GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC
    AGGTTACTAC AGATCTCTCC ATCTAGTGGA ACAACAGTTA CTTCCGTTGT GGGATTTTCC
    TGTGATGGGC TGAGACCAGA AGCCATAAGG CAAGATCCTA CTCGGAAAGG CTCAGTGGTC
    AATGTGAACC CCACGAACAC TAGGCCACAG AGTGATACCC CCGAGATTCG TAAATACAAG
    AAGAGATTTA ACTCAGAGAT CCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTTGGC
    ACAGAGAGTG GCCTGATGCT CTTGGACAGA AGTGGCCAAG GGAAAGTGTA CCCTCTGATC
    AGCCGGAGAC GGTTCCAGCA AATGGATGTA CTTGAGGGCC TGAATGTCCT GGTGACAATA
    TCTGGCAAAA AGGATAAGTT ACGCGTCTAC TATTTATCCT GGTTAAGAAA TAAAATACTT
    CATAATGATC CGGAAGTTGA GAAGAAGCAG GGGTGGACCA CCGTGGGCGA CCTGGAAGGG
    TGCGTGCACT ATAAAGTCGT AAAATATGAA AGAATCAAGT TTCTGGTAAT CGCTTTGAAG
    AGTTCTGTGG AAGTCTATGC ATGGGCACCG AAGCCATATC ACAAATTTAT GGCCTTTAAG
    TCATTTGGAG AACTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTCGA GGAAGGCCAG
    AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCCGGC
    TCAGTCTATG ACATTTATCT ACCAACACAC ATTCAGTGTA CCATCAAACC CCATGCAATC
    ATTATCCTCC CCAACACGGA TGGAATGGAG CTTCTGGTGT GCTACGAAGA TGAAGGGGTT
    TATGTGAACA CGTACGGAAG AATCACCAAG GACGTGGTTC TGCAGTGGGG AGAGATGCCA
    ACATCTGTAG CATACATCCG GTCGAATCAG ACAATGGGCT GGGGAGAGAA GGCAATAGAG
    ATACGCTCTG TGGAAACTGG TCATTTGGAC GGTGTGTTTA TGCACAAAAG GGCTCAGAGA
    CTAAAGTTCC TGTGTGAACG TAATGACAAG GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC
    AGCAGCCAGG TCTACTTCAT GACCTTAGGC AGGACTTCCC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62707
    Clone ID Related Accession (Same CDS sequence) XM_008767013.2
    Accession Version XM_008767013.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3687bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767013.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTTCC TGTGAGAACG
    ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA
    AATAGCCAAG CAGGACAGAG AAATTCCACC AGCAGTATTG AGCCCCGGCT TCTATGGGAG
    AGAGTGGAAA AGCTGGTCCC CAGGCCAGGC AGTGGCAGCT CTTCAGGGTC CAGCAACTCA
    GGATCCCAGC CTGGCTCCCA TCCTGGGTCT CAGAGTGGCT CTGGTGAACG CTTCAGAGTG
    AGATCATCAT CCAAGTCTGA AGGTTCTCCA TCTCAGCGCC TTGAAAATGC AGCCAAAAAA
    CCTGAAGATA AGAAAGAAGT ATTTCGACCT CTCAAACCTG CTGGAGAAGT GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTGCGGC CACCTCACAA AGTGACAGAC
    TATTCCTCCT CCAGTGAGGA GTCTGGGACC ACGGATGAGG AGGAGGAGGA TGTGGAACAG
    GAGGGGGCTG ATGATTCCAC CTCGGGGCCA GAGGACACCA GAGCAGCGTC GTCTCTGAAT
    CTGAGCAACG GTGAGACAGA ATCTGTGAAA ACAATGATTG TTCATGATGA CGTAGAAAGT
    GAACCAGCCA TGACCCCATC CAAGGAGGGC ACCCTAATCG TCCGCCAGAG TACAGTTGAC
    CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA
    GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCTTCCTC CCCATCCTCC
    AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG
    ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT
    ATAGACCCCA GGTTACTACA GATCTCTCCA TCTAGTGGAA CAACAGTTAC TTCCGTTGTG
    GGATTTTCCT GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC
    TCAGTGGTCA ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT
    AAATACAAGA AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAATTTG
    CTAGTTGGCA CAGAGAGTGG CCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTGTAC
    CCTCTGATCA GCCGGAGACG GTTCCAGCAA ATGGATGTAC TTGAGGGCCT GAATGTCCTG
    GTGACAATAT CTGGCAAAAA GGATAAGTTA CGCGTCTACT ATTTATCCTG GTTAAGAAAT
    AAAATACTTC ATAATGATCC GGAAGTTGAG AAGAAGCAGG GGTGGACCAC CGTGGGCGAC
    CTGGAAGGGT GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATC
    GCTTTGAAGA GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG
    GCCTTTAAGT CATTTGGAGA ACTGGTACAT AAGCCATTAC TGGTGGATCT CACTGTCGAG
    GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG
    GATTCCGGCT CAGTCTATGA CATTTATCTA CCAACACACG TAAGAAAGAA CCCACACTCT
    ATGATTCAGT GTACCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGAATG
    GAGCTTCTGG TGTGCTACGA AGATGAAGGG GTTTATGTGA ACACGTACGG AAGAATCACC
    AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCAACATCTG TAGCATACAT CCGGTCGAAT
    CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGCT CTGTGGAAAC TGGTCATTTG
    GACGGTGTGT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGTAATGAC
    AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC GGCAGCAGCC AGGTCTACTT CATGACCTTA
    GGCAGGACTT CCCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765235.2
    CDS209..3895
    Translation

    Target ORF information:

    RefSeq Version XM_008767013.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767013.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTTCC TGTGAGAACG
    ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA
    AATAGCCAAG CAGGACAGAG AAATTCCACC AGCAGTATTG AGCCCCGGCT TCTATGGGAG
    AGAGTGGAAA AGCTGGTCCC CAGGCCAGGC AGTGGCAGCT CTTCAGGGTC CAGCAACTCA
    GGATCCCAGC CTGGCTCCCA TCCTGGGTCT CAGAGTGGCT CTGGTGAACG CTTCAGAGTG
    AGATCATCAT CCAAGTCTGA AGGTTCTCCA TCTCAGCGCC TTGAAAATGC AGCCAAAAAA
    CCTGAAGATA AGAAAGAAGT ATTTCGACCT CTCAAACCTG CTGGAGAAGT GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTGCGGC CACCTCACAA AGTGACAGAC
    TATTCCTCCT CCAGTGAGGA GTCTGGGACC ACGGATGAGG AGGAGGAGGA TGTGGAACAG
    GAGGGGGCTG ATGATTCCAC CTCGGGGCCA GAGGACACCA GAGCAGCGTC GTCTCTGAAT
    CTGAGCAACG GTGAGACAGA ATCTGTGAAA ACAATGATTG TTCATGATGA CGTAGAAAGT
    GAACCAGCCA TGACCCCATC CAAGGAGGGC ACCCTAATCG TCCGCCAGAG TACAGTTGAC
    CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA
    GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCTTCCTC CCCATCCTCC
    AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG
    ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT
    ATAGACCCCA GGTTACTACA GATCTCTCCA TCTAGTGGAA CAACAGTTAC TTCCGTTGTG
    GGATTTTCCT GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC
    TCAGTGGTCA ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT
    AAATACAAGA AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAATTTG
    CTAGTTGGCA CAGAGAGTGG CCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTGTAC
    CCTCTGATCA GCCGGAGACG GTTCCAGCAA ATGGATGTAC TTGAGGGCCT GAATGTCCTG
    GTGACAATAT CTGGCAAAAA GGATAAGTTA CGCGTCTACT ATTTATCCTG GTTAAGAAAT
    AAAATACTTC ATAATGATCC GGAAGTTGAG AAGAAGCAGG GGTGGACCAC CGTGGGCGAC
    CTGGAAGGGT GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATC
    GCTTTGAAGA GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG
    GCCTTTAAGT CATTTGGAGA ACTGGTACAT AAGCCATTAC TGGTGGATCT CACTGTCGAG
    GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG
    GATTCCGGCT CAGTCTATGA CATTTATCTA CCAACACACG TAAGAAAGAA CCCACACTCT
    ATGATTCAGT GTACCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGAATG
    GAGCTTCTGG TGTGCTACGA AGATGAAGGG GTTTATGTGA ACACGTACGG AAGAATCACC
    AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCAACATCTG TAGCATACAT CCGGTCGAAT
    CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGCT CTGTGGAAAC TGGTCATTTG
    GACGGTGTGT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGTAATGAC
    AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC GGCAGCAGCC AGGTCTACTT CATGACCTTA
    GGCAGGACTT CCCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62708
    Clone ID Related Accession (Same CDS sequence) XM_017596344.1
    Accession Version XM_017596344.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3684bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTTCC TGTGAGAACG
    ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA
    AATAGCCAAG CAGGACAGAG AAATTCCACC AGTATTGAGC CCCGGCTTCT ATGGGAGAGA
    GTGGAAAAGC TGGTCCCCAG GCCAGGCAGT GGCAGCTCTT CAGGGTCCAG CAACTCAGGA
    TCCCAGCCTG GCTCCCATCC TGGGTCTCAG AGTGGCTCTG GTGAACGCTT CAGAGTGAGA
    TCATCATCCA AGTCTGAAGG TTCTCCATCT CAGCGCCTTG AAAATGCAGC CAAAAAACCT
    GAAGATAAGA AAGAAGTATT TCGACCTCTC AAACCTGCTG GAGAAGTGGA CTTGACTGCG
    TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTGCGGCCAC CTCACAAAGT GACAGACTAT
    TCCTCCTCCA GTGAGGAGTC TGGGACCACG GATGAGGAGG AGGAGGATGT GGAACAGGAG
    GGGGCTGATG ATTCCACCTC GGGGCCAGAG GACACCAGAG CAGCGTCGTC TCTGAATCTG
    AGCAACGGTG AGACAGAATC TGTGAAAACA ATGATTGTTC ATGATGACGT AGAAAGTGAA
    CCAGCCATGA CCCCATCCAA GGAGGGCACC CTAATCGTCC GCCAGAGTAC AGTTGACCAA
    AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CTTCCTCCCC ATCCTCCAGC
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGGT TACTACAGAT CTCTCCATCT AGTGGAACAA CAGTTACTTC CGTTGTGGGA
    TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC ATAAGGCAAG ATCCTACTCG GAAAGGCTCA
    GTGGTCAATG TGAACCCCAC GAACACTAGG CCACAGAGTG ATACCCCCGA GATTCGTAAA
    TACAAGAAGA GATTTAACTC AGAGATCCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA
    GTTGGCACAG AGAGTGGCCT GATGCTCTTG GACAGAAGTG GCCAAGGGAA AGTGTACCCT
    CTGATCAGCC GGAGACGGTT CCAGCAAATG GATGTACTTG AGGGCCTGAA TGTCCTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGC GTCTACTATT TATCCTGGTT AAGAAATAAA
    ATACTTCATA ATGATCCGGA AGTTGAGAAG AAGCAGGGGT GGACCACCGT GGGCGACCTG
    GAAGGGTGCG TGCACTATAA AGTCGTAAAA TATGAAAGAA TCAAGTTTCT GGTAATCGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCATGG GCACCGAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAACT GGTACATAAG CCATTACTGG TGGATCTCAC TGTCGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT
    TCCGGCTCAG TCTATGACAT TTATCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG
    ATTCAGTGTA CCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGAATGGAG
    CTTCTGGTGT GCTACGAAGA TGAAGGGGTT TATGTGAACA CGTACGGAAG AATCACCAAG
    GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATACATCCG GTCGAATCAG
    ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGCTCTG TGGAAACTGG TCATTTGGAC
    GGTGTGTTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG TAATGACAAG
    GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC AGCAGCCAGG TCTACTTCAT GACCTTAGGC
    AGGACTTCCC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017451833.1
    CDS209..3892
    Translation

    Target ORF information:

    RefSeq Version XM_017596344.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017596344.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTTCC TGTGAGAACG
    ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA
    AATAGCCAAG CAGGACAGAG AAATTCCACC AGTATTGAGC CCCGGCTTCT ATGGGAGAGA
    GTGGAAAAGC TGGTCCCCAG GCCAGGCAGT GGCAGCTCTT CAGGGTCCAG CAACTCAGGA
    TCCCAGCCTG GCTCCCATCC TGGGTCTCAG AGTGGCTCTG GTGAACGCTT CAGAGTGAGA
    TCATCATCCA AGTCTGAAGG TTCTCCATCT CAGCGCCTTG AAAATGCAGC CAAAAAACCT
    GAAGATAAGA AAGAAGTATT TCGACCTCTC AAACCTGCTG GAGAAGTGGA CTTGACTGCG
    TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTGCGGCCAC CTCACAAAGT GACAGACTAT
    TCCTCCTCCA GTGAGGAGTC TGGGACCACG GATGAGGAGG AGGAGGATGT GGAACAGGAG
    GGGGCTGATG ATTCCACCTC GGGGCCAGAG GACACCAGAG CAGCGTCGTC TCTGAATCTG
    AGCAACGGTG AGACAGAATC TGTGAAAACA ATGATTGTTC ATGATGACGT AGAAAGTGAA
    CCAGCCATGA CCCCATCCAA GGAGGGCACC CTAATCGTCC GCCAGAGTAC AGTTGACCAA
    AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CTTCCTCCCC ATCCTCCAGC
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGGT TACTACAGAT CTCTCCATCT AGTGGAACAA CAGTTACTTC CGTTGTGGGA
    TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC ATAAGGCAAG ATCCTACTCG GAAAGGCTCA
    GTGGTCAATG TGAACCCCAC GAACACTAGG CCACAGAGTG ATACCCCCGA GATTCGTAAA
    TACAAGAAGA GATTTAACTC AGAGATCCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA
    GTTGGCACAG AGAGTGGCCT GATGCTCTTG GACAGAAGTG GCCAAGGGAA AGTGTACCCT
    CTGATCAGCC GGAGACGGTT CCAGCAAATG GATGTACTTG AGGGCCTGAA TGTCCTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGC GTCTACTATT TATCCTGGTT AAGAAATAAA
    ATACTTCATA ATGATCCGGA AGTTGAGAAG AAGCAGGGGT GGACCACCGT GGGCGACCTG
    GAAGGGTGCG TGCACTATAA AGTCGTAAAA TATGAAAGAA TCAAGTTTCT GGTAATCGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCATGG GCACCGAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAACT GGTACATAAG CCATTACTGG TGGATCTCAC TGTCGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT
    TCCGGCTCAG TCTATGACAT TTATCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG
    ATTCAGTGTA CCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGAATGGAG
    CTTCTGGTGT GCTACGAAGA TGAAGGGGTT TATGTGAACA CGTACGGAAG AATCACCAAG
    GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATACATCCG GTCGAATCAG
    ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGCTCTG TGGAAACTGG TCATTTGGAC
    GGTGTGTTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG TAATGACAAG
    GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC AGCAGCCAGG TCTACTTCAT GACCTTAGGC
    AGGACTTCCC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62709
    Clone ID Related Accession (Same CDS sequence) XM_008767014.2
    Accession Version XM_008767014.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT
    AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG
    CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT
    GCCCTGCAGA GACCAGCGGA GCCACAGGTT CCTGTGAGAA CGACATCTCG TTCCCCTGTC
    CTGTCCCGTC GAGATTCTCC ACTGCAGGGC AGTGGGCAGC AAAATAGCCA AGCAGGACAG
    AGAAATTCCA CCAGCAGTAT TGAGCCCCGG CTTCTATGGG AGAGAGTGGA AAAGCTGGTC
    CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG TCCAGCAACT CAGGATCCCA GCCTGGCTCC
    CATCCTGGGT CTCAGAGTGG CTCTGGTGAA CGCTTCAGAG TGAGATCATC ATCCAAGTCT
    GAAGGTTCTC CATCTCAGCG CCTTGAAAAT GCAGCCAAAA AACCTGAAGA TAAGAAAGAA
    GTATTTCGAC CTCTCAAACC TGCTGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG
    GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG
    ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG
    CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG
    AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG
    GGCACCCTAA TCGTCCGCCA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGGTTACTAC AGATCTCTCC ATCTAGTGGA
    ACAACAGTTA CTTCCGTTGT GGGATTTTCC TGTGATGGGC TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CTCGGAAAGG CTCAGTGGTC AATGTGAACC CCACGAACAC TAGGCCACAG
    AGTGATACCC CCGAGATTCG TAAATACAAG AAGAGATTTA ACTCAGAGAT CCTGTGTGCT
    GCCTTATGGG GAGTGAATTT GCTAGTTGGC ACAGAGAGTG GCCTGATGCT CTTGGACAGA
    AGTGGCCAAG GGAAAGTGTA CCCTCTGATC AGCCGGAGAC GGTTCCAGCA AATGGATGTA
    CTTGAGGGCC TGAATGTCCT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGCGTCTAC
    TATTTATCCT GGTTAAGAAA TAAAATACTT CATAATGATC CGGAAGTTGA GAAGAAGCAG
    GGGTGGACCA CCGTGGGCGA CCTGGAAGGG TGCGTGCACT ATAAAGTCGT AAAATATGAA
    AGAATCAAGT TTCTGGTAAT CGCTTTGAAG AGTTCTGTGG AAGTCTATGC ATGGGCACCG
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AACTGGTACA TAAGCCATTA
    CTGGTGGATC TCACTGTCGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT
    GGATTCCATG CTGTTGATGT GGATTCCGGC TCAGTCTATG ACATTTATCT ACCAACACAC
    ATTCAGTGTA CCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGAATGGAG
    CTTCTGGTGT GCTACGAAGA TGAAGGGGTT TATGTGAACA CGTACGGAAG AATCACCAAG
    GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATACATCCG GTCGAATCAG
    ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGCTCTG TGGAAACTGG TCATTTGGAC
    GGTGTGTTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG TAATGACAAG
    GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC AGCAGCCAGG TCTACTTCAT GACCTTAGGC
    AGGACTTCCC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765236.2
    CDS208..3831
    Translation

    Target ORF information:

    RefSeq Version XM_008767014.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767014.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT
    AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG
    CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT
    GCCCTGCAGA GACCAGCGGA GCCACAGGTT CCTGTGAGAA CGACATCTCG TTCCCCTGTC
    CTGTCCCGTC GAGATTCTCC ACTGCAGGGC AGTGGGCAGC AAAATAGCCA AGCAGGACAG
    AGAAATTCCA CCAGCAGTAT TGAGCCCCGG CTTCTATGGG AGAGAGTGGA AAAGCTGGTC
    CCCAGGCCAG GCAGTGGCAG CTCTTCAGGG TCCAGCAACT CAGGATCCCA GCCTGGCTCC
    CATCCTGGGT CTCAGAGTGG CTCTGGTGAA CGCTTCAGAG TGAGATCATC ATCCAAGTCT
    GAAGGTTCTC CATCTCAGCG CCTTGAAAAT GCAGCCAAAA AACCTGAAGA TAAGAAAGAA
    GTATTTCGAC CTCTCAAACC TGCTGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG
    GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG
    ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG
    CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG
    AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG
    GGCACCCTAA TCGTCCGCCA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGGTTACTAC AGATCTCTCC ATCTAGTGGA
    ACAACAGTTA CTTCCGTTGT GGGATTTTCC TGTGATGGGC TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CTCGGAAAGG CTCAGTGGTC AATGTGAACC CCACGAACAC TAGGCCACAG
    AGTGATACCC CCGAGATTCG TAAATACAAG AAGAGATTTA ACTCAGAGAT CCTGTGTGCT
    GCCTTATGGG GAGTGAATTT GCTAGTTGGC ACAGAGAGTG GCCTGATGCT CTTGGACAGA
    AGTGGCCAAG GGAAAGTGTA CCCTCTGATC AGCCGGAGAC GGTTCCAGCA AATGGATGTA
    CTTGAGGGCC TGAATGTCCT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGCGTCTAC
    TATTTATCCT GGTTAAGAAA TAAAATACTT CATAATGATC CGGAAGTTGA GAAGAAGCAG
    GGGTGGACCA CCGTGGGCGA CCTGGAAGGG TGCGTGCACT ATAAAGTCGT AAAATATGAA
    AGAATCAAGT TTCTGGTAAT CGCTTTGAAG AGTTCTGTGG AAGTCTATGC ATGGGCACCG
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AACTGGTACA TAAGCCATTA
    CTGGTGGATC TCACTGTCGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT
    GGATTCCATG CTGTTGATGT GGATTCCGGC TCAGTCTATG ACATTTATCT ACCAACACAC
    ATTCAGTGTA CCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGAATGGAG
    CTTCTGGTGT GCTACGAAGA TGAAGGGGTT TATGTGAACA CGTACGGAAG AATCACCAAG
    GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATACATCCG GTCGAATCAG
    ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGCTCTG TGGAAACTGG TCATTTGGAC
    GGTGTGTTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG TAATGACAAG
    GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC AGCAGCCAGG TCTACTTCAT GACCTTAGGC
    AGGACTTCCC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62710
    Clone ID Related Accession (Same CDS sequence) XM_017596345.1
    Accession Version XM_017596345.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3864bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT
    AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG
    CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT
    GCCCTGCAGA GACCAGCGGA GCCACAGGTA CAGTGGTCCC ACCTGGCATC TCTCAAGAAC
    AATGTCTCCC CTGTCTCGAG ATCCCATTCC TTCAGTGACC CTTCTCCCAA ATTCGCACAC
    CACCATCTCC GCTCTCAGGA CCCATGTCCA CCTTCCCGCA GTGAGGGGCT CAGTCAGAGC
    TCTGACTCTA AGTCGGAGGT ACCTGAGCCC ACCCAAAAGG CTTGGTCTAG ATCAGACAGT
    GACGAGGTGC CTCCAAGGGT TCCTGTGAGA ACGACATCTC GTTCCCCTGT CCTGTCCCGT
    CGAGATTCTC CACTGCAGGG CAGTGGGCAG CAAAATAGCC AAGCAGGACA GAGAAATTCC
    ACCAGCAGTA TTGAGCCCCG GCTTCTATGG GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA
    GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC TCAGGATCCC AGCCTGGCTC CCATCCTGGG
    TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA GTGAGATCAT CATCCAAGTC TGAAGGTTCT
    CCATCTCAGC GCCTTGAAAA TGCAGCCAAA AAACCTGAAG ATAAGAAAGA AGTATTTCGA
    CCTCTCAAAC CTGCTGGAGA AGTGGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG
    GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG
    ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG
    CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG
    AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG
    GGCACCCTAA TCGTCCGCCA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGGTTACTAC AGATCTCTCC ATCTAGTGGA
    ACAACAGTTA CTTCCGTTGT GGGATTTTCC TGTGATGGGC TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CTCGGAAAGG CTCAGTGGTC AATGTGAACC CCACGAACAC TAGGCCACAG
    AGTGATACCC CCGAGATTCG TAAATACAAG AAGAGATTTA ACTCAGAGAT CCTGTGTGCT
    GCCTTATGGG GAGTGAATTT GCTAGTTGGC ACAGAGAGTG GCCTGATGCT CTTGGACAGA
    AGTGGCCAAG GGAAAGTGTA CCCTCTGATC AGCCGGAGAC GGTTCCAGCA AATGGATGTA
    CTTGAGGGCC TGAATGTCCT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGCGTCTAC
    TATTTATCCT GGTTAAGAAA TAAAATACTT CATAATGATC CGGAAGTTGA GAAGAAGCAG
    GGGTGGACCA CCGTGGGCGA CCTGGAAGGG TGCGTGCACT ATAAAGTCGT AAAATATGAA
    AGAATCAAGT TTCTGGTAAT CGCTTTGAAG AGTTCTGTGG AAGTCTATGC ATGGGCACCG
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AACTGGTACA TAAGCCATTA
    CTGGTGGATC TCACTGTCGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT
    GGATTCCATG CTGTTGATGT GGATTCCGGC TCAGTCTATG ACATTTATCT ACCAACACAC
    ATTCAGTGTA CCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGAATGGAG
    CTTCTGGTGT GCTACGAAGA TGAAGGGGTT TATGTGAACA CGTACGGAAG AATCACCAAG
    GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATACATCCG GTCGAATCAG
    ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGCTCTG TGGAAACTGG TCATTTGGAC
    GGTGTGTTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG TAATGACAAG
    GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC AGCAGCCAGG TCTACTTCAT GACCTTAGGC
    AGGACTTCCC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017451834.1
    CDS210..4073
    Translation

    Target ORF information:

    RefSeq Version XM_017596345.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017596345.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTGGA AGATAGATTT
    AGAAAGACTA ACCACAGCTC CCCTGAGGCC CAGGCTAAGC AGACAGGCAG AGGACTGGAG
    CCACCCGTGC CTTCCCGGTC AGAATCTTTT TCCAATGGCA ACTCAGAGTC TGTGCACCCT
    GCCCTGCAGA GACCAGCGGA GCCACAGGTA CAGTGGTCCC ACCTGGCATC TCTCAAGAAC
    AATGTCTCCC CTGTCTCGAG ATCCCATTCC TTCAGTGACC CTTCTCCCAA ATTCGCACAC
    CACCATCTCC GCTCTCAGGA CCCATGTCCA CCTTCCCGCA GTGAGGGGCT CAGTCAGAGC
    TCTGACTCTA AGTCGGAGGT ACCTGAGCCC ACCCAAAAGG CTTGGTCTAG ATCAGACAGT
    GACGAGGTGC CTCCAAGGGT TCCTGTGAGA ACGACATCTC GTTCCCCTGT CCTGTCCCGT
    CGAGATTCTC CACTGCAGGG CAGTGGGCAG CAAAATAGCC AAGCAGGACA GAGAAATTCC
    ACCAGCAGTA TTGAGCCCCG GCTTCTATGG GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA
    GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC TCAGGATCCC AGCCTGGCTC CCATCCTGGG
    TCTCAGAGTG GCTCTGGTGA ACGCTTCAGA GTGAGATCAT CATCCAAGTC TGAAGGTTCT
    CCATCTCAGC GCCTTGAAAA TGCAGCCAAA AAACCTGAAG ATAAGAAAGA AGTATTTCGA
    CCTCTCAAAC CTGCTGGAGA AGTGGACTTG ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG
    GAAGATGTGC GGCCACCTCA CAAAGTGACA GACTATTCCT CCTCCAGTGA GGAGTCTGGG
    ACCACGGATG AGGAGGAGGA GGATGTGGAA CAGGAGGGGG CTGATGATTC CACCTCGGGG
    CCAGAGGACA CCAGAGCAGC GTCGTCTCTG AATCTGAGCA ACGGTGAGAC AGAATCTGTG
    AAAACAATGA TTGTTCATGA TGACGTAGAA AGTGAACCAG CCATGACCCC ATCCAAGGAG
    GGCACCCTAA TCGTCCGCCA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGGTTACTAC AGATCTCTCC ATCTAGTGGA
    ACAACAGTTA CTTCCGTTGT GGGATTTTCC TGTGATGGGC TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CTCGGAAAGG CTCAGTGGTC AATGTGAACC CCACGAACAC TAGGCCACAG
    AGTGATACCC CCGAGATTCG TAAATACAAG AAGAGATTTA ACTCAGAGAT CCTGTGTGCT
    GCCTTATGGG GAGTGAATTT GCTAGTTGGC ACAGAGAGTG GCCTGATGCT CTTGGACAGA
    AGTGGCCAAG GGAAAGTGTA CCCTCTGATC AGCCGGAGAC GGTTCCAGCA AATGGATGTA
    CTTGAGGGCC TGAATGTCCT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGCGTCTAC
    TATTTATCCT GGTTAAGAAA TAAAATACTT CATAATGATC CGGAAGTTGA GAAGAAGCAG
    GGGTGGACCA CCGTGGGCGA CCTGGAAGGG TGCGTGCACT ATAAAGTCGT AAAATATGAA
    AGAATCAAGT TTCTGGTAAT CGCTTTGAAG AGTTCTGTGG AAGTCTATGC ATGGGCACCG
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AACTGGTACA TAAGCCATTA
    CTGGTGGATC TCACTGTCGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT
    GGATTCCATG CTGTTGATGT GGATTCCGGC TCAGTCTATG ACATTTATCT ACCAACACAC
    ATTCAGTGTA CCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGAATGGAG
    CTTCTGGTGT GCTACGAAGA TGAAGGGGTT TATGTGAACA CGTACGGAAG AATCACCAAG
    GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACATCTGTAG CATACATCCG GTCGAATCAG
    ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGCTCTG TGGAAACTGG TCATTTGGAC
    GGTGTGTTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG TAATGACAAG
    GTGTTCTTTG CTTCCGTCCG CTCTGGCGGC AGCAGCCAGG TCTACTTCAT GACCTTAGGC
    AGGACTTCCC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62711
    Clone ID Related Accession (Same CDS sequence) XM_008767015.2
    Accession Version XM_008767015.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTACA GTGGTCCCAC
    CTGGCATCTC TCAAGAACAA TGTCTCCCCT GTCTCGAGAT CCCATTCCTT CAGTGACCCT
    TCTCCCAAAT TCGCACACCA CCATCTCCGC TCTCAGGACC CATGTCCACC TTCCCGCAGT
    GAGGGGCTCA GTCAGAGCTC TGACTCTAAG TCGGAGGTAC CTGAGCCCAC CCAAAAGGCT
    TGGTCTAGAT CAGACAGTGA CGAGGTGCCT CCAAGGGTTC CTGTGAGAAC GACATCTCGT
    TCCCCTGTCC TGTCCCGTCG AGATTCTCCA CTGCAGGGCA GTGGGCAGCA AAATAGCCAA
    GCAGGACAGA GAAATTCCAC CAGCAGTATT GAGCCCCGGC TTCTATGGGA GAGAGTGGAA
    AAGCTGGTCC CCAGGCCAGG CAGTGGCAGC TCTTCAGGGT CCAGCAACTC AGGATCCCAG
    CCTGGCTCCC ATCCTGGGTC TCAGAGTGGC TCTGGTGAAC GCTTCAGAGT GAGATCATCA
    TCCAAGTCTG AAGGTTCTCC ATCTCAGCGC CTTGAAAATG CAGCCAAAAA ACCTGAAGAT
    AAGAAAGAAG TATTTCGACC TCTCAAACCT GCTGGAGAAG TGGACTTGAC TGCGTTGGCC
    AAAGAGCTTC GAGCAGTGGA AGATGTGCGG CCACCTCACA AAGTGACAGA CTATTCCTCC
    TCCAGTGAGG AGTCTGGGAC CACGGATGAG GAGGAGGAGG ATGTGGAACA GGAGGGGGCT
    GATGATTCCA CCTCGGGGCC AGAGGACACC AGAGCAGCGT CGTCTCTGAA TCTGAGCAAC
    GGTGAGACAG AATCTGTGAA AACAATGATT GTTCATGATG ACGTAGAAAG TGAACCAGCC
    ATGACCCCAT CCAAGGAGGG CACCCTAATC GTCCGCCAGA CTCAGTCCGC TAGTAGCACA
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG GTTACTACAG
    ATCTCTCCAT CTAGTGGAAC AACAGTTACT TCCGTTGTGG GATTTTCCTG TGATGGGCTG
    AGACCAGAAG CCATAAGGCA AGATCCTACT CGGAAAGGCT CAGTGGTCAA TGTGAACCCC
    ACGAACACTA GGCCACAGAG TGATACCCCC GAGATTCGTA AATACAAGAA GAGATTTAAC
    TCAGAGATCC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTTGGCAC AGAGAGTGGC
    CTGATGCTCT TGGACAGAAG TGGCCAAGGG AAAGTGTACC CTCTGATCAG CCGGAGACGG
    TTCCAGCAAA TGGATGTACT TGAGGGCCTG AATGTCCTGG TGACAATATC TGGCAAAAAG
    GATAAGTTAC GCGTCTACTA TTTATCCTGG TTAAGAAATA AAATACTTCA TAATGATCCG
    GAAGTTGAGA AGAAGCAGGG GTGGACCACC GTGGGCGACC TGGAAGGGTG CGTGCACTAT
    AAAGTCGTAA AATATGAAAG AATCAAGTTT CTGGTAATCG CTTTGAAGAG TTCTGTGGAA
    GTCTATGCAT GGGCACCGAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA
    CTGGTACATA AGCCATTACT GGTGGATCTC ACTGTCGAGG AAGGCCAGAG GTTGAAAGTG
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCCGGCTC AGTCTATGAC
    ATTTATCTAC CAACACACAT TCAGTGTACC ATCAAACCCC ATGCAATCAT TATCCTCCCC
    AACACGGATG GAATGGAGCT TCTGGTGTGC TACGAAGATG AAGGGGTTTA TGTGAACACG
    TACGGAAGAA TCACCAAGGA CGTGGTTCTG CAGTGGGGAG AGATGCCAAC ATCTGTAGCA
    TACATCCGGT CGAATCAGAC AATGGGCTGG GGAGAGAAGG CAATAGAGAT ACGCTCTGTG
    GAAACTGGTC ATTTGGACGG TGTGTTTATG CACAAAAGGG CTCAGAGACT AAAGTTCCTG
    TGTGAACGTA ATGACAAGGT GTTCTTTGCT TCCGTCCGCT CTGGCGGCAG CAGCCAGGTC
    TACTTCATGA CCTTAGGCAG GACTTCCCTT CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765237.2
    CDS209..3910
    Translation

    Target ORF information:

    RefSeq Version XM_008767015.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767015.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTACA GTGGTCCCAC
    CTGGCATCTC TCAAGAACAA TGTCTCCCCT GTCTCGAGAT CCCATTCCTT CAGTGACCCT
    TCTCCCAAAT TCGCACACCA CCATCTCCGC TCTCAGGACC CATGTCCACC TTCCCGCAGT
    GAGGGGCTCA GTCAGAGCTC TGACTCTAAG TCGGAGGTAC CTGAGCCCAC CCAAAAGGCT
    TGGTCTAGAT CAGACAGTGA CGAGGTGCCT CCAAGGGTTC CTGTGAGAAC GACATCTCGT
    TCCCCTGTCC TGTCCCGTCG AGATTCTCCA CTGCAGGGCA GTGGGCAGCA AAATAGCCAA
    GCAGGACAGA GAAATTCCAC CAGCAGTATT GAGCCCCGGC TTCTATGGGA GAGAGTGGAA
    AAGCTGGTCC CCAGGCCAGG CAGTGGCAGC TCTTCAGGGT CCAGCAACTC AGGATCCCAG
    CCTGGCTCCC ATCCTGGGTC TCAGAGTGGC TCTGGTGAAC GCTTCAGAGT GAGATCATCA
    TCCAAGTCTG AAGGTTCTCC ATCTCAGCGC CTTGAAAATG CAGCCAAAAA ACCTGAAGAT
    AAGAAAGAAG TATTTCGACC TCTCAAACCT GCTGGAGAAG TGGACTTGAC TGCGTTGGCC
    AAAGAGCTTC GAGCAGTGGA AGATGTGCGG CCACCTCACA AAGTGACAGA CTATTCCTCC
    TCCAGTGAGG AGTCTGGGAC CACGGATGAG GAGGAGGAGG ATGTGGAACA GGAGGGGGCT
    GATGATTCCA CCTCGGGGCC AGAGGACACC AGAGCAGCGT CGTCTCTGAA TCTGAGCAAC
    GGTGAGACAG AATCTGTGAA AACAATGATT GTTCATGATG ACGTAGAAAG TGAACCAGCC
    ATGACCCCAT CCAAGGAGGG CACCCTAATC GTCCGCCAGA CTCAGTCCGC TAGTAGCACA
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG GTTACTACAG
    ATCTCTCCAT CTAGTGGAAC AACAGTTACT TCCGTTGTGG GATTTTCCTG TGATGGGCTG
    AGACCAGAAG CCATAAGGCA AGATCCTACT CGGAAAGGCT CAGTGGTCAA TGTGAACCCC
    ACGAACACTA GGCCACAGAG TGATACCCCC GAGATTCGTA AATACAAGAA GAGATTTAAC
    TCAGAGATCC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTTGGCAC AGAGAGTGGC
    CTGATGCTCT TGGACAGAAG TGGCCAAGGG AAAGTGTACC CTCTGATCAG CCGGAGACGG
    TTCCAGCAAA TGGATGTACT TGAGGGCCTG AATGTCCTGG TGACAATATC TGGCAAAAAG
    GATAAGTTAC GCGTCTACTA TTTATCCTGG TTAAGAAATA AAATACTTCA TAATGATCCG
    GAAGTTGAGA AGAAGCAGGG GTGGACCACC GTGGGCGACC TGGAAGGGTG CGTGCACTAT
    AAAGTCGTAA AATATGAAAG AATCAAGTTT CTGGTAATCG CTTTGAAGAG TTCTGTGGAA
    GTCTATGCAT GGGCACCGAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA
    CTGGTACATA AGCCATTACT GGTGGATCTC ACTGTCGAGG AAGGCCAGAG GTTGAAAGTG
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCCGGCTC AGTCTATGAC
    ATTTATCTAC CAACACACAT TCAGTGTACC ATCAAACCCC ATGCAATCAT TATCCTCCCC
    AACACGGATG GAATGGAGCT TCTGGTGTGC TACGAAGATG AAGGGGTTTA TGTGAACACG
    TACGGAAGAA TCACCAAGGA CGTGGTTCTG CAGTGGGGAG AGATGCCAAC ATCTGTAGCA
    TACATCCGGT CGAATCAGAC AATGGGCTGG GGAGAGAAGG CAATAGAGAT ACGCTCTGTG
    GAAACTGGTC ATTTGGACGG TGTGTTTATG CACAAAAGGG CTCAGAGACT AAAGTTCCTG
    TGTGAACGTA ATGACAAGGT GTTCTTTGCT TCCGTCCGCT CTGGCGGCAG CAGCCAGGTC
    TACTTCATGA CCTTAGGCAG GACTTCCCTT CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62712
    Clone ID Related Accession (Same CDS sequence) XM_008767016.2
    Accession Version XM_008767016.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3663bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767016.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTTCC TGTGAGAACG
    ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA
    AATAGCCAAG CAGGACAGAG AAATTCCACC AGCAGTATTG AGCCCCGGCT TCTATGGGAG
    AGAGTGGAAA AGCTGGTCCC CAGGCCAGGC AGTGGCAGCT CTTCAGGGTC CAGCAACTCA
    GGATCCCAGC CTGGCTCCCA TCCTGGGTCT CAGAGTGGCT CTGGTGAACG CTTCAGAGTG
    AGATCATCAT CCAAGTCTGA AGGTTCTCCA TCTCAGCGCC TTGAAAATGC AGCCAAAAAA
    CCTGAAGATA AGAAAGAAGT ATTTCGACCT CTCAAACCTG CTGGAGAAGT GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTGCGGC CACCTCACAA AGTGACAGAC
    TATTCCTCCT CCAGTGAGGA GTCTGGGACC ACGGATGAGG AGGAGGAGGA TGTGGAACAG
    GAGGGGGCTG ATGATTCCAC CTCGGGGCCA GAGGACACCA GAGCAGCGTC GTCTCTGAAT
    CTGAGCAACG GTGAGACAGA ATCTGTGAAA ACAATGATTG TTCATGATGA CGTAGAAAGT
    GAACCAGCCA TGACCCCATC CAAGGAGGGC ACCCTAATCG TCCGCCAGAG TACAGTTGAC
    CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA
    GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCTTCCTC CCCATCCTCC
    AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG
    ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT
    ATAGACCCCA GGTTACTACA GATCTCTCCA TCTAGTGGAA CAACAGTTAC TTCCGTTGTG
    GGATTTTCCT GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC
    TCAGTGGTCA ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT
    AAATACAAGA AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAATTTG
    CTAGTTGGCA CAGAGAGTGG CCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTGTAC
    CCTCTGATCA GCCGGAGACG GTTCCAGCAA ATGGATGTAC TTGAGGGCCT GAATGTCCTG
    GTGACAATAT CTGGCAAAAA GGATAAGTTA CGCGTCTACT ATTTATCCTG GTTAAGAAAT
    AAAATACTTC ATAATGATCC GGAAGTTGAG AAGAAGCAGG GGTGGACCAC CGTGGGCGAC
    CTGGAAGGGT GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATC
    GCTTTGAAGA GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG
    GCCTTTAAGT CATTTGGAGA ACTGGTACAT AAGCCATTAC TGGTGGATCT CACTGTCGAG
    GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG
    GATTCCGGCT CAGTCTATGA CATTTATCTA CCAACACACA TTCAGTGTAC CATCAAACCC
    CATGCAATCA TTATCCTCCC CAACACGGAT GGAATGGAGC TTCTGGTGTG CTACGAAGAT
    GAAGGGGTTT ATGTGAACAC GTACGGAAGA ATCACCAAGG ACGTGGTTCT GCAGTGGGGA
    GAGATGCCAA CATCTGTAGC ATACATCCGG TCGAATCAGA CAATGGGCTG GGGAGAGAAG
    GCAATAGAGA TACGCTCTGT GGAAACTGGT CATTTGGACG GTGTGTTTAT GCACAAAAGG
    GCTCAGAGAC TAAAGTTCCT GTGTGAACGT AATGACAAGG TGTTCTTTGC TTCCGTCCGC
    TCTGGCGGCA GCAGCCAGGT CTACTTCATG ACCTTAGGCA GGACTTCCCT TCTGAGCTGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008765238.2
    CDS207..3869
    Translation

    Target ORF information:

    RefSeq Version XM_008767016.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008767016.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTTCC TGTGAGAACG
    ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA
    AATAGCCAAG CAGGACAGAG AAATTCCACC AGCAGTATTG AGCCCCGGCT TCTATGGGAG
    AGAGTGGAAA AGCTGGTCCC CAGGCCAGGC AGTGGCAGCT CTTCAGGGTC CAGCAACTCA
    GGATCCCAGC CTGGCTCCCA TCCTGGGTCT CAGAGTGGCT CTGGTGAACG CTTCAGAGTG
    AGATCATCAT CCAAGTCTGA AGGTTCTCCA TCTCAGCGCC TTGAAAATGC AGCCAAAAAA
    CCTGAAGATA AGAAAGAAGT ATTTCGACCT CTCAAACCTG CTGGAGAAGT GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTGCGGC CACCTCACAA AGTGACAGAC
    TATTCCTCCT CCAGTGAGGA GTCTGGGACC ACGGATGAGG AGGAGGAGGA TGTGGAACAG
    GAGGGGGCTG ATGATTCCAC CTCGGGGCCA GAGGACACCA GAGCAGCGTC GTCTCTGAAT
    CTGAGCAACG GTGAGACAGA ATCTGTGAAA ACAATGATTG TTCATGATGA CGTAGAAAGT
    GAACCAGCCA TGACCCCATC CAAGGAGGGC ACCCTAATCG TCCGCCAGAG TACAGTTGAC
    CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA
    GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCTTCCTC CCCATCCTCC
    AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG
    ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT
    ATAGACCCCA GGTTACTACA GATCTCTCCA TCTAGTGGAA CAACAGTTAC TTCCGTTGTG
    GGATTTTCCT GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC
    TCAGTGGTCA ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT
    AAATACAAGA AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAATTTG
    CTAGTTGGCA CAGAGAGTGG CCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTGTAC
    CCTCTGATCA GCCGGAGACG GTTCCAGCAA ATGGATGTAC TTGAGGGCCT GAATGTCCTG
    GTGACAATAT CTGGCAAAAA GGATAAGTTA CGCGTCTACT ATTTATCCTG GTTAAGAAAT
    AAAATACTTC ATAATGATCC GGAAGTTGAG AAGAAGCAGG GGTGGACCAC CGTGGGCGAC
    CTGGAAGGGT GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATC
    GCTTTGAAGA GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG
    GCCTTTAAGT CATTTGGAGA ACTGGTACAT AAGCCATTAC TGGTGGATCT CACTGTCGAG
    GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG
    GATTCCGGCT CAGTCTATGA CATTTATCTA CCAACACACA TTCAGTGTAC CATCAAACCC
    CATGCAATCA TTATCCTCCC CAACACGGAT GGAATGGAGC TTCTGGTGTG CTACGAAGAT
    GAAGGGGTTT ATGTGAACAC GTACGGAAGA ATCACCAAGG ACGTGGTTCT GCAGTGGGGA
    GAGATGCCAA CATCTGTAGC ATACATCCGG TCGAATCAGA CAATGGGCTG GGGAGAGAAG
    GCAATAGAGA TACGCTCTGT GGAAACTGGT CATTTGGACG GTGTGTTTAT GCACAAAAGG
    GCTCAGAGAC TAAAGTTCCT GTGTGAACGT AATGACAAGG TGTTCTTTGC TTCCGTCCGC
    TCTGGCGGCA GCAGCCAGGT CTACTTCATG ACCTTAGGCA GGACTTCCCT TCTGAGCTGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa62713
    Clone ID Related Accession (Same CDS sequence) XM_017596346.1
    Accession Version XM_017596346.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTTCC TGTGAGAACG
    ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA
    AATAGCCAAG CAGGACAGAG AAATTCCACC AGTATTGAGC CCCGGCTTCT ATGGGAGAGA
    GTGGAAAAGC TGGTCCCCAG GCCAGGCAGT GGCAGCTCTT CAGGGTCCAG CAACTCAGGA
    TCCCAGCCTG GCTCCCATCC TGGGTCTCAG AGTGGCTCTG GTGAACGCTT CAGAGTGAGA
    TCATCATCCA AGTCTGAAGG TTCTCCATCT CAGCGCCTTG AAAATGCAGC CAAAAAACCT
    GAAGATAAGA AAGAAGTATT TCGACCTCTC AAACCTGCTG GAGAAGTGGA CTTGACTGCG
    TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTGCGGCCAC CTCACAAAGT GACAGACTAT
    TCCTCCTCCA GTGAGGAGTC TGGGACCACG GATGAGGAGG AGGAGGATGT GGAACAGGAG
    GGGGCTGATG ATTCCACCTC GGGGCCAGAG GACACCAGAG CAGCGTCGTC TCTGAATCTG
    AGCAACGGTG AGACAGAATC TGTGAAAACA ATGATTGTTC ATGATGACGT AGAAAGTGAA
    CCAGCCATGA CCCCATCCAA GGAGGGCACC CTAATCGTCC GCCAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGGTTA
    CTACAGATCT CTCCATCTAG TGGAACAACA GTTACTTCCG TTGTGGGATT TTCCTGTGAT
    GGGCTGAGAC CAGAAGCCAT AAGGCAAGAT CCTACTCGGA AAGGCTCAGT GGTCAATGTG
    AACCCCACGA ACACTAGGCC ACAGAGTGAT ACCCCCGAGA TTCGTAAATA CAAGAAGAGA
    TTTAACTCAG AGATCCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT TGGCACAGAG
    AGTGGCCTGA TGCTCTTGGA CAGAAGTGGC CAAGGGAAAG TGTACCCTCT GATCAGCCGG
    AGACGGTTCC AGCAAATGGA TGTACTTGAG GGCCTGAATG TCCTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGCGT CTACTATTTA TCCTGGTTAA GAAATAAAAT ACTTCATAAT
    GATCCGGAAG TTGAGAAGAA GCAGGGGTGG ACCACCGTGG GCGACCTGGA AGGGTGCGTG
    CACTATAAAG TCGTAAAATA TGAAAGAATC AAGTTTCTGG TAATCGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCATGGGC ACCGAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAACTGG TACATAAGCC ATTACTGGTG GATCTCACTG TCGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC CGGCTCAGTC
    TATGACATTT ATCTACCAAC ACACGTAAGA AAGAACCCAC ACTCTATGAT TCAGTGTACC
    ATCAAACCCC ATGCAATCAT TATCCTCCCC AACACGGATG GAATGGAGCT TCTGGTGTGC
    TACGAAGATG AAGGGGTTTA TGTGAACACG TACGGAAGAA TCACCAAGGA CGTGGTTCTG
    CAGTGGGGAG AGATGCCAAC ATCTGTAGCA TACATCCGGT CGAATCAGAC AATGGGCTGG
    GGAGAGAAGG CAATAGAGAT ACGCTCTGTG GAAACTGGTC ATTTGGACGG TGTGTTTATG
    CACAAAAGGG CTCAGAGACT AAAGTTCCTG TGTGAACGTA ATGACAAGGT GTTCTTTGCT
    TCCGTCCGCT CTGGCGGCAG CAGCCAGGTC TACTTCATGA CCTTAGGCAG GACTTCCCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017451835.1
    CDS209..3700
    Translation

    Target ORF information:

    RefSeq Version XM_017596346.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017596346.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAACG ACTCTCCGGC TAAAAGCTTG GTGGACATCG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCGGCCATCA AGGTTATGGA TGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA
    AATATTGCAA CATACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA CGATGACCAG
    CTCTGGCTCG TTATGGAGTT TTGCGGGGCT GGTTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CGCTCAAAGA AGACTGGATT GCTTACATCT CCCGGGAAAT CCTCAGGGGG
    TTGGCCCATC TCCACATTCA CCATGTCATT CACCGAGACA TCAAGGGGCA GAACGTGCTG
    CTGACTGAGA ACGCGGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC ATTCATAGGC ACACCATACT GGATGGCTCC GGAGGTCATC
    GCCTGCGACG AGAATCCAGA TGCCACTTAC GACTACAGAA GTGACCTCTG GTCTTGTGGC
    ATCACGGCCA TCGAGATGGC AGAAGGAGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTCATAGA AGGCTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGG AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GTGGCAGTGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCG ACTCTGCGAC GTGATTTCCT GAGGCTGCAG
    CAAGAGAACA AGGAACGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GGAGAGAGCG TGAAGCCAGG
    AGGCAGCAGG AACGTGAGCA GCGGCGGCGA GAACAGGAGG AGAAGAGGCG TCTGGAGGAA
    CTGGAAAGGC GGCGTAAGGA AGAGGAAGAG AGGAGACGGG CAGAAGACGA GAAGAGGCGA
    GTGGAGCGGG AGCAGGAGTA CATCAGGCGG CAGCTAGAGG AAGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCTATGTTAC TGCACGACCA CAGGAGGCCA
    CACGCACAAC AGCCGCCACC CCCGCAGCAG CAGGACAGGA GCAAACCCAG CTATCACGCT
    CCAGAGCCCA AGCCTCACTA CGACCCTGCT GACAGAGCTC GGGAGGTTCC TGTGAGAACG
    ACATCTCGTT CCCCTGTCCT GTCCCGTCGA GATTCTCCAC TGCAGGGCAG TGGGCAGCAA
    AATAGCCAAG CAGGACAGAG AAATTCCACC AGTATTGAGC CCCGGCTTCT ATGGGAGAGA
    GTGGAAAAGC TGGTCCCCAG GCCAGGCAGT GGCAGCTCTT CAGGGTCCAG CAACTCAGGA
    TCCCAGCCTG GCTCCCATCC TGGGTCTCAG AGTGGCTCTG GTGAACGCTT CAGAGTGAGA
    TCATCATCCA AGTCTGAAGG TTCTCCATCT CAGCGCCTTG AAAATGCAGC CAAAAAACCT
    GAAGATAAGA AAGAAGTATT TCGACCTCTC AAACCTGCTG GAGAAGTGGA CTTGACTGCG
    TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTGCGGCCAC CTCACAAAGT GACAGACTAT
    TCCTCCTCCA GTGAGGAGTC TGGGACCACG GATGAGGAGG AGGAGGATGT GGAACAGGAG
    GGGGCTGATG ATTCCACCTC GGGGCCAGAG GACACCAGAG CAGCGTCGTC TCTGAATCTG
    AGCAACGGTG AGACAGAATC TGTGAAAACA ATGATTGTTC ATGATGACGT AGAAAGTGAA
    CCAGCCATGA CCCCATCCAA GGAGGGCACC CTAATCGTCC GCCAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGGTTA
    CTACAGATCT CTCCATCTAG TGGAACAACA GTTACTTCCG TTGTGGGATT TTCCTGTGAT
    GGGCTGAGAC CAGAAGCCAT AAGGCAAGAT CCTACTCGGA AAGGCTCAGT GGTCAATGTG
    AACCCCACGA ACACTAGGCC ACAGAGTGAT ACCCCCGAGA TTCGTAAATA CAAGAAGAGA
    TTTAACTCAG AGATCCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT TGGCACAGAG
    AGTGGCCTGA TGCTCTTGGA CAGAAGTGGC CAAGGGAAAG TGTACCCTCT GATCAGCCGG
    AGACGGTTCC AGCAAATGGA TGTACTTGAG GGCCTGAATG TCCTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGCGT CTACTATTTA TCCTGGTTAA GAAATAAAAT ACTTCATAAT
    GATCCGGAAG TTGAGAAGAA GCAGGGGTGG ACCACCGTGG GCGACCTGGA AGGGTGCGTG
    CACTATAAAG TCGTAAAATA TGAAAGAATC AAGTTTCTGG TAATCGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCATGGGC ACCGAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAACTGG TACATAAGCC ATTACTGGTG GATCTCACTG TCGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC CGGCTCAGTC
    TATGACATTT ATCTACCAAC ACACGTAAGA AAGAACCCAC ACTCTATGAT TCAGTGTACC
    ATCAAACCCC ATGCAATCAT TATCCTCCCC AACACGGATG GAATGGAGCT TCTGGTGTGC
    TACGAAGATG AAGGGGTTTA TGTGAACACG TACGGAAGAA TCACCAAGGA CGTGGTTCTG
    CAGTGGGGAG AGATGCCAAC ATCTGTAGCA TACATCCGGT CGAATCAGAC AATGGGCTGG
    GGAGAGAAGG CAATAGAGAT ACGCTCTGTG GAAACTGGTC ATTTGGACGG TGTGTTTATG
    CACAAAAGGG CTCAGAGACT AAAGTTCCTG TGTGAACGTA ATGACAAGGT GTTCTTTGCT
    TCCGTCCGCT CTGGCGGCAG CAGCCAGGTC TACTTCATGA CCTTAGGCAG GACTTCCCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa42317
    Clone ID Related Accession (Same CDS sequence) NM_001106904.1
    Accession Version NM_001106904.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3537bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-18
    Organism Rattus norvegicus(Norway rat)
    Product mitogen-activated protein kinase kinase kinase kinase 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473965.2. On or before Oct 4, 2007 this sequence version replaced XM_001058712.1, XM_001058590.1, XM_001058649.1, XM_001058485.1, XM_001058541.1, XM_217381.4, XM_001058422.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATGTCA CCGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAC TATGGTGCTT TCATTAAGAA GAGCCCTCCA
    GGACACGATG ACCAGCTCTG GCTCGTTATG GAGTTTTGCG GGGCTGGTTC CATCACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAAGAAGACT GGATTGCTTA CATCTCCCGG
    GAAATCCTCA GGGGGTTGGC CCATCTCCAC ATTCACCATG TCATTCACCG AGACATCAAG
    GGGCAGAACG TGCTGCTGAC TGAGAACGCG GAGGTGAAAC TTGTTGATTT TGGTGTAAGC
    GCTCAGCTGG ACAGGACGGT TGGACGGAGA AATACATTCA TAGGCACACC ATACTGGATG
    GCTCCGGAGG TCATCGCCTG CGACGAGAAT CCAGATGCCA CTTACGACTA CAGAAGTGAC
    CTCTGGTCTT GTGGCATCAC GGCCATCGAG ATGGCAGAAG GAGCCCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCGCT GTTTCTCATC CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTCAGCTTC ATAGAAGGCT GCCTGGTGAA GAATTACATG
    CAGCGGCCCT CTACAGAGCA GCTTTTAAAA CACCCTTTCA TAAGGGATCA GCCCAATGAA
    AGGCAGGTTC GAATCCAGCT TAAGGATCAC ATAGACCGGA CCAGGAAGAA GAGAGGCGAG
    AAAGATGAGA CGGAGTACGA GTACAGTGGC AGTGAGGAGG AGGAGGAGGA AGTGCCTGAG
    CAGGAGGGAG AGCCAAGTTC CATCGTCAAT GTGCCTGGAG AGTCGACTCT GCGACGTGAT
    TTCCTGAGGC TGCAGCAAGA GAACAAGGAA CGGTCTGAGG CTCTGCGGAG ACAGCAGCTT
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTATAAGA GGCAGCTGCT GGCTGAGAGG
    CAGAAGCGGA TTGAACAGCA GAAAGAACAG AGGAGGCGGC TGGAAGAGCA ACAAAGGAGA
    GAGCGTGAAG CCAGGAGGCA GCAGGAACGT GAGCAGCGGC GGCGAGAACA GGAGGAGAAG
    AGGCGTCTGG AGGAACTGGA AAGGCGGCGT AAGGAAGAGG AAGAGAGGAG ACGGGCAGAA
    GACGAGAAGA GGCGAGTGGA GCGGGAGCAG GAGTACATCA GGCGGCAGCT AGAGGAAGAG
    CAGCGGCACC TGGAGATCCT GCAGCAGCAG CTGCTCCAGG AGCAGGCTAT GTTACTGCAC
    GACCACAGGA GGCCACACGC ACAACAGCCG CCACCCCCGC AGCAGCAGGA CAGGAGCAAA
    CCCAGCTATC ACGCTCCAGA GCCCAAGCCT CACTACGACC CTGCTGACAG AGCTCGGGAG
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT
    TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG
    CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA
    TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC
    AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGT AAGGATTGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG
    ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG
    GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT
    CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA
    GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGGTTAC TACAGATCTC TCCATCTAGT GGAACAACAG TTACTTCCGT TGTGGGATTT
    TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG
    GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC
    AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT
    GGCACAGAGA GTGGCCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT GTACCCTCTG
    ATCAGCCGGA GACGGTTCCA GCAAATGGAT GTACTTGAGG GCCTGAATGT CCTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGCGTC TACTATTTAT CCTGGTTAAG AAATAAAATA
    CTTCATAATG ATCCGGAAGT TGAGAAGAAG CAGGGGTGGA CCACCGTGGG CGACCTGGAA
    GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATCGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAACTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT CGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCC
    GGCTCAGTCT ATGACATTTA TCTACCAACA CACATTCAGT GTACCATCAA ACCCCATGCA
    ATCATTATCC TCCCCAACAC GGATGGAATG GAGCTTCTGG TGTGCTACGA AGATGAAGGG
    GTTTATGTGA ACACGTACGG AAGAATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG
    CCAACATCTG TAGCATACAT CCGGTCGAAT CAGACAATGG GCTGGGGAGA GAAGGCAATA
    GAGATACGCT CTGTGGAAAC TGGTCATTTG GACGGTGTGT TTATGCACAA AAGGGCTCAG
    AGACTAAAGT TCCTGTGTGA ACGTAATGAC AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC
    GGCAGCAGCC AGGTCTACTT CATGACCTTA GGCAGGACTT CCCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001100374.1
    CDS111..3647
    Translation

    Target ORF information:

    RefSeq Version NM_001106904.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001106904.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGATGTCA CCGAGGATGA AGAGGAAGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACATAC TATGGTGCTT TCATTAAGAA GAGCCCTCCA
    GGACACGATG ACCAGCTCTG GCTCGTTATG GAGTTTTGCG GGGCTGGTTC CATCACAGAC
    CTTGTGAAGA ACACCAAAGG GAACACGCTC AAAGAAGACT GGATTGCTTA CATCTCCCGG
    GAAATCCTCA GGGGGTTGGC CCATCTCCAC ATTCACCATG TCATTCACCG AGACATCAAG
    GGGCAGAACG TGCTGCTGAC TGAGAACGCG GAGGTGAAAC TTGTTGATTT TGGTGTAAGC
    GCTCAGCTGG ACAGGACGGT TGGACGGAGA AATACATTCA TAGGCACACC ATACTGGATG
    GCTCCGGAGG TCATCGCCTG CGACGAGAAT CCAGATGCCA CTTACGACTA CAGAAGTGAC
    CTCTGGTCTT GTGGCATCAC GGCCATCGAG ATGGCAGAAG GAGCCCCCCC TCTCTGTGAC
    ATGCATCCAA TGAGAGCGCT GTTTCTCATC CCCAGAAACC CTCCTCCCAG GCTGAAGTCA
    AAAAAATGGT CAAAGAAATT TTTCAGCTTC ATAGAAGGCT GCCTGGTGAA GAATTACATG
    CAGCGGCCCT CTACAGAGCA GCTTTTAAAA CACCCTTTCA TAAGGGATCA GCCCAATGAA
    AGGCAGGTTC GAATCCAGCT TAAGGATCAC ATAGACCGGA CCAGGAAGAA GAGAGGCGAG
    AAAGATGAGA CGGAGTACGA GTACAGTGGC AGTGAGGAGG AGGAGGAGGA AGTGCCTGAG
    CAGGAGGGAG AGCCAAGTTC CATCGTCAAT GTGCCTGGAG AGTCGACTCT GCGACGTGAT
    TTCCTGAGGC TGCAGCAAGA GAACAAGGAA CGGTCTGAGG CTCTGCGGAG ACAGCAGCTT
    CTGCAGGAGC AGCAGCTCCG GGAGCAGGAG GAGTATAAGA GGCAGCTGCT GGCTGAGAGG
    CAGAAGCGGA TTGAACAGCA GAAAGAACAG AGGAGGCGGC TGGAAGAGCA ACAAAGGAGA
    GAGCGTGAAG CCAGGAGGCA GCAGGAACGT GAGCAGCGGC GGCGAGAACA GGAGGAGAAG
    AGGCGTCTGG AGGAACTGGA AAGGCGGCGT AAGGAAGAGG AAGAGAGGAG ACGGGCAGAA
    GACGAGAAGA GGCGAGTGGA GCGGGAGCAG GAGTACATCA GGCGGCAGCT AGAGGAAGAG
    CAGCGGCACC TGGAGATCCT GCAGCAGCAG CTGCTCCAGG AGCAGGCTAT GTTACTGCAC
    GACCACAGGA GGCCACACGC ACAACAGCCG CCACCCCCGC AGCAGCAGGA CAGGAGCAAA
    CCCAGCTATC ACGCTCCAGA GCCCAAGCCT CACTACGACC CTGCTGACAG AGCTCGGGAG
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTCT CCCCTGTCTC GAGATCCCAT
    TCCTTCAGTG ACCCTTCTCC CAAATTCGCA CACCACCATC TCCGCTCTCA GGACCCATGT
    CCACCTTCCC GCAGTGAGGG GCTCAGTCAG AGCTCTGACT CTAAGTCGGA GGTACCTGAG
    CCCACCCAAA AGGCTTGGTC TAGATCAGAC AGTGACGAGG TGCCTCCAAG GGTTCCTGTG
    AGAACGACAT CTCGTTCCCC TGTCCTGTCC CGTCGAGATT CTCCACTGCA GGGCAGTGGG
    CAGCAAAATA GCCAAGCAGG ACAGAGAAAT TCCACCAGCA GTATTGAGCC CCGGCTTCTA
    TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GTGGCTCTGG TGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCTC AGCGCCTTGA AAATGCAGCC
    AAAAAACCTG AAGATAAGAA AGAAGTATTT CGACCTCTCA AACCTGCTGT AAGGATTGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TGCGGCCACC TCACAAAGTG
    ACAGACTATT CCTCCTCCAG TGAGGAGTCT GGGACCACGG ATGAGGAGGA GGAGGATGTG
    GAACAGGAGG GGGCTGATGA TTCCACCTCG GGGCCAGAGG ACACCAGAGC AGCGTCGTCT
    CTGAATCTGA GCAACGGTGA GACAGAATCT GTGAAAACAA TGATTGTTCA TGATGACGTA
    GAAAGTGAAC CAGCCATGAC CCCATCCAAG GAGGGCACCC TAATCGTCCG CCAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGGTTAC TACAGATCTC TCCATCTAGT GGAACAACAG TTACTTCCGT TGTGGGATTT
    TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG
    GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC
    AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTT
    GGCACAGAGA GTGGCCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT GTACCCTCTG
    ATCAGCCGGA GACGGTTCCA GCAAATGGAT GTACTTGAGG GCCTGAATGT CCTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGCGTC TACTATTTAT CCTGGTTAAG AAATAAAATA
    CTTCATAATG ATCCGGAAGT TGAGAAGAAG CAGGGGTGGA CCACCGTGGG CGACCTGGAA
    GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATCGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAACTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT CGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCC
    GGCTCAGTCT ATGACATTTA TCTACCAACA CACATTCAGT GTACCATCAA ACCCCATGCA
    ATCATTATCC TCCCCAACAC GGATGGAATG GAGCTTCTGG TGTGCTACGA AGATGAAGGG
    GTTTATGTGA ACACGTACGG AAGAATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG
    CCAACATCTG TAGCATACAT CCGGTCGAAT CAGACAATGG GCTGGGGAGA GAAGGCAATA
    GAGATACGCT CTGTGGAAAC TGGTCATTTG GACGGTGTGT TTATGCACAA AAGGGCTCAG
    AGACTAAAGT TCCTGTGTGA ACGTAATGAC AAGGTGTTCT TTGCTTCCGT CCGCTCTGGC
    GGCAGCAGCC AGGTCTACTT CATGACCTTA GGCAGGACTT CCCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.