ORF » Species Summary » Bos taurus » MAP4K4 cDNA ORF clone

MAP4K4 cDNA ORF clone, Bos taurus(cattle)

The following MAP4K4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP4K4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa47360 XM_015465311.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
OBa47360 XM_010809817.2
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $713.30
$1019.00
OBa220746 XM_024999322.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa220747 XM_024999323.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa220748 XM_024999324.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa220749 XM_024999325.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa220750 XM_024999326.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa220751 XM_024999327.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa220752 XM_024999328.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220753 XM_024999329.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220754 XM_024999330.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220754 XM_024999331.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220755 XM_024999332.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220756 XM_024999333.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220757 XM_024999334.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220758 XM_024999335.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220759 XM_024999336.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220760 XM_024999337.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220761 XM_024999338.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220762 XM_024999339.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220763 XM_024999340.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220764 XM_024999341.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220765 XM_024999342.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220766 XM_024999343.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220767 XM_024999344.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220768 XM_024999345.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220769 XM_024999346.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220770 XM_024999347.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220771 XM_024999348.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220772 XM_024999349.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220773 XM_024999350.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220774 XM_024999351.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220775 XM_024999352.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa220776 XM_024999353.1
Latest version!		 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    MAP4K4 ( XM_015465311.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320797.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), mRNA.
    Price & Availability↑
    CloneID OBa47360
    Clone ID Related Accession (Same CDS sequence) XM_015465311.1 , XM_010809817.2
    Accession Version XM_015465311.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645889.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGTTCT GTGGGGCCGG ATCCATCACG GACCTGGTGA AGAACACCAA GGGCAACACG 
    CTAAAGGAAG ACTGGATAGC CTACATCTCC AGGGAGATCC TTCGGGGCCT GGCCCATCTC 
    CACATCCATC ACGTGATCCA CCGCGACATC AAGGGCCAGA ATGTGCTGCT GACCGAGAAC 
    GCAGAGGTGA AGCTGGTGGA CTTCGGGGTG AGTGCCCAGC TGGACAGGAC CGTGGGGAGG 
    AGGAACACGT TCATAGGCAC CCCGTACTGG ATGGCCCCTG AGGTCATTGC CTGTGACGAG 
    AACCCCGACG CCACCTACGA TTACAGGAGT GACCTCTGGT CCTGTGGCAT CACTGCCATT 
    GAGATGGCTG AAGGCGCTCC CCCTCTCTGC GACATGCATC CCATGAGAGC CCTGTTTCTC 
    ATCCCCCGGA ATCCTCCTCC CCGGCTGAAG TCCAAAAAAT GGTCCAAGAA GTTTTTTAGT 
    TTTATAGAAG GATGCCTGGT GAAGAATTAC ATGCAGCGGC CCTCCACCGA GCAGCTTCTG 
    AAGCATCCTT TCATTAGGGA TCAGCCGAAC GAAAGGCAGG TTAGAATCCA GCTTAAGGAC 
    CATATAGACC GGACCAGGAA GAAGAGAGGA GAGAAAGATG AAACCGAGTA TGAGTACAGT 
    GGGAGTGAGG AAGAGGAGGA GGAGGCGCCT GAGCAGGAGG GGGAGCCCAG CTCCATCGTC 
    AACGTGCCGG GGGAGTCGAC GCTGCGCCGG GACTTCCTGC GGCTCCAGCA GGAGAACAAG 
    GAGCGCTCTG AGGCCCTGAG GAGGCAGCAG CTCCTGCAGG AGCAGCAGCT CCGCGAGCAG 
    GAGGAGTATA AACGGCAGCT GCTGGCGGAG AGGCAGAAGC GGATCGAGCA GCAGAAGGAG 
    CAGAGGCGGC GGCTGGAAGA GCAACAGAGG AGAGAGCGGG AAGCCAGAAG GCAGCAGGAA 
    CGTGAGCAGC GGAGGCGGGA ACAGGAGGAG AAGAGGCGGC TGGAGGAGCT GGAGAGACGA 
    CGGAAGGAGG AGGAGGAGCG GAGGCGGGCG GAGGAGGAGA AGAGGAGAGT GGAGAGGGAG 
    CAGGAGTATA TCCGACGGCA GCTGGAAGAG GAACAGCGTC ACCTGGAACT GCTTCAGCAG 
    CAGCTGCTCC AGGAGCAGGC CATGCTACTG GAGTGTCGCT GGCGGGAGCT GGAGGAGCAC 
    CGGCAGGCAG AGCGGCTCCA GAGGCAGCTG CAGCAAGAAC AAGCCTATCT CCTGTCTCTA 
    CAGCACGACC CCCGGAGGCC GCCCCCGCCG CCAGACAGGA GCAAGGCTGG CCTGCACGAG 
    CCCAAGGCCC CGCACGAGCC CGCTGACCGG GCCCGTGAGG TGGAAGATAG ATTTAGGAAA 
    ACTAACCACA GCTCCCCCGA AGCCCAGTCT AAGCAGACGG GCCGAGTGTT GGAACCTCCA 
    GTGCCTTCCA GATCAGAGTC TTTTTCGAGT GGCAACTCCG AGTCTGTGCA CCCTGCCCTG 
    CAGAGACCCG CCGAGCCACA GGTACAGTGG TCCCACCTGG CATCTCTCAA GAACAATGTT 
    TCCCCTGTCT CGAGATCCCA TTCCTTCAGT GACCCTTCTC CTCCCAAATT TGCACACCAC 
    CATCTTCGCT CTCAGGACCC CTGCCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGTTCT 
    GACTCTAAGG CGGAGGTGCC CGACCCAGCC CAGAAGGCTT GGTCTAGATC AGACAGTGAC 
    GAGGTGCCTC CACGGGTCCC TGTGAGAACA ACATCACGGT CCCCTGTCCT GTCCCGCCGG 
    GATTCCCCAC TGCAGGGTAG TGGGCAGCAG AGTAACCAGG CTGGTCAGAG AAACTCCACC 
    AGTAGCATCG AGCCGAGGCT GCTCTGGGAG AGAGTGGAGA AGCTGGCGCC CAGGCCGGGC 
    AGTGGCAGCT CCTCGGGGTC CAGCAACTCG GGGTCCCAGC CCGGCTCCCA CCCAGGGTCT 
    CAGAGTGGCT CCGGGGAGCG CTTCAGAGTG AGATCATCAT CCAAATCTGA AGGCTCGCCA 
    TCTCAGCGCC TTGAAAACGC AGCAAAAAAG CCTGAAGATA AAAAGGAAGT GTTTAGACCT 
    CTCAAGCCTG CTGGTGAAGT GGACCTGACG GCTCTGGCCA AAGAGCTGCG GGCCGTGGAG 
    GATGTGCGGC CGCCGCACAA GGTGACAGAC TACTCCTCCT CCAGCGAGGA GTCGGGGACA 
    ACGGACGAGG AGGACGACGA CGTGGAGCAG GAGGGGGCCG AGGAGCCTAC GTCTGGCCCG 
    GAGGACACCA GAGCTGCATC ATCTCTGAAC TTGAGCAATG GTGAAACAGA GTCCGTGAAA 
    ACCATGGTTG TCCATGACGA CGTGGAGAGT GAACCAGCCC TGACCCCGTC CAAAGAGGGC 
    ACGCTGATCG TCCGCCAGAG TGCAGTTGAC CAAAAGCGCG CCAGCCATCA TGAGAGCAAT 
    GGCTTTGCGG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC 
    ACTTCCTCCA CCTCCTCTTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG 
    ACACCCCAGG ACAAGCTCAC TACTAATGAG ACTCAGTCCG CTAGTAACAC ACTCCAGAAA 
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
    TCTAGCGGGA CAACAGTGAC TTCTGTGGTG GGGTTTTCCT GTGATGGAAT GAGACCTGAG 
    GCCATGAGGC AAGATCCTAC GCGGAAGGGC TCAGTGGTCA ATGTCAATCC CACCAATACT 
    CGGCCCCAGA GCGACACCCC AGAGATTCGC AAATACAAGA AGAGGTTTAA CTCCGAGATC 
    CTGTGCGCAG CCTTGTGGGG AGTGAACTTG TTGGTGGGTA CCGAGAGTGG CCTGATGCTG 
    CTGGACAGAA GTGGCCAAGG GAAGGTGTAT CCTCTGATCA ACAGAAGGCG ATTTCAGCAG 
    ATGGACGTCC TGGAAGGCTT GAACGTCTTA GTGACGATAT CTGGCAAAAA GGATAAGTTA 
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAACGACCC GGAGGTTGAG 
    AAGAAGCAGG GCTGGACGAC AGTGGGGGAC CTGGAAGGCT GTGTGCATTA TAAAGTTGTA 
    AAATATGAAA GGATCAAATT TCTGGTCATT GCTTTGAAGA ACTCTGTGGA AGTCTACGCA 
    TGGGCACCCA AGCCATATCA CAAATTCATG GCCTTTAAGT CATTTGGTGA GCTGGTGCAC 
    AAGCCGTTGC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGCTGAAAGT GATCTATGGA 
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GACTCAGGAT CGGTCTATGA CATTTACCTA 
    CCAACACACA TCCAATGTAG CATCAAACCC CACGCAATCA TCATCCTCCC CAACACAGAT 
    GGCATGGAGC TCCTGGTGTG CTATGAAGAT GAAGGGGTTT ACGTGAACAC GTACGGGAGG 
    ATCACCAAGG ACGTGGTTCT GCAGTGGGGA GAGATGCCAA CGTCTGTAGC ATATATTCGA 
    TCCAATCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGATCTGT GGAAACTGGC 
    CACTTGGATG GCGTGTTTAT GCATAAAAGG GCTCAAAGGC TGAAGTTCTT GTGTGAACGT 
    AATGACAAGG TGTTCTTTGC CTCCGTCCGG TCTGGGGGCA GCAGCCAGGT TTACTTCATG 
    ACTCTCGGCA GGACCTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320797.1
    CDS34..3846
    Translation

    Target ORF information:

    RefSeq Version XM_015465311.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_015465311.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGTTCT GTGGGGCCGG ATCCATCACG GACCTGGTGA AGAACACCAA GGGCAACACG 
    CTAAAGGAAG ACTGGATAGC CTACATCTCC AGGGAGATCC TTCGGGGCCT GGCCCATCTC 
    CACATCCATC ACGTGATCCA CCGCGACATC AAGGGCCAGA ATGTGCTGCT GACCGAGAAC 
    GCAGAGGTGA AGCTGGTGGA CTTCGGGGTG AGTGCCCAGC TGGACAGGAC CGTGGGGAGG 
    AGGAACACGT TCATAGGCAC CCCGTACTGG ATGGCCCCTG AGGTCATTGC CTGTGACGAG 
    AACCCCGACG CCACCTACGA TTACAGGAGT GACCTCTGGT CCTGTGGCAT CACTGCCATT 
    GAGATGGCTG AAGGCGCTCC CCCTCTCTGC GACATGCATC CCATGAGAGC CCTGTTTCTC 
    ATCCCCCGGA ATCCTCCTCC CCGGCTGAAG TCCAAAAAAT GGTCCAAGAA GTTTTTTAGT 
    TTTATAGAAG GATGCCTGGT GAAGAATTAC ATGCAGCGGC CCTCCACCGA GCAGCTTCTG 
    AAGCATCCTT TCATTAGGGA TCAGCCGAAC GAAAGGCAGG TTAGAATCCA GCTTAAGGAC 
    CATATAGACC GGACCAGGAA GAAGAGAGGA GAGAAAGATG AAACCGAGTA TGAGTACAGT 
    GGGAGTGAGG AAGAGGAGGA GGAGGCGCCT GAGCAGGAGG GGGAGCCCAG CTCCATCGTC 
    AACGTGCCGG GGGAGTCGAC GCTGCGCCGG GACTTCCTGC GGCTCCAGCA GGAGAACAAG 
    GAGCGCTCTG AGGCCCTGAG GAGGCAGCAG CTCCTGCAGG AGCAGCAGCT CCGCGAGCAG 
    GAGGAGTATA AACGGCAGCT GCTGGCGGAG AGGCAGAAGC GGATCGAGCA GCAGAAGGAG 
    CAGAGGCGGC GGCTGGAAGA GCAACAGAGG AGAGAGCGGG AAGCCAGAAG GCAGCAGGAA 
    CGTGAGCAGC GGAGGCGGGA ACAGGAGGAG AAGAGGCGGC TGGAGGAGCT GGAGAGACGA 
    CGGAAGGAGG AGGAGGAGCG GAGGCGGGCG GAGGAGGAGA AGAGGAGAGT GGAGAGGGAG 
    CAGGAGTATA TCCGACGGCA GCTGGAAGAG GAACAGCGTC ACCTGGAACT GCTTCAGCAG 
    CAGCTGCTCC AGGAGCAGGC CATGCTACTG GAGTGTCGCT GGCGGGAGCT GGAGGAGCAC 
    CGGCAGGCAG AGCGGCTCCA GAGGCAGCTG CAGCAAGAAC AAGCCTATCT CCTGTCTCTA 
    CAGCACGACC CCCGGAGGCC GCCCCCGCCG CCAGACAGGA GCAAGGCTGG CCTGCACGAG 
    CCCAAGGCCC CGCACGAGCC CGCTGACCGG GCCCGTGAGG TGGAAGATAG ATTTAGGAAA 
    ACTAACCACA GCTCCCCCGA AGCCCAGTCT AAGCAGACGG GCCGAGTGTT GGAACCTCCA 
    GTGCCTTCCA GATCAGAGTC TTTTTCGAGT GGCAACTCCG AGTCTGTGCA CCCTGCCCTG 
    CAGAGACCCG CCGAGCCACA GGTACAGTGG TCCCACCTGG CATCTCTCAA GAACAATGTT 
    TCCCCTGTCT CGAGATCCCA TTCCTTCAGT GACCCTTCTC CTCCCAAATT TGCACACCAC 
    CATCTTCGCT CTCAGGACCC CTGCCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGTTCT 
    GACTCTAAGG CGGAGGTGCC CGACCCAGCC CAGAAGGCTT GGTCTAGATC AGACAGTGAC 
    GAGGTGCCTC CACGGGTCCC TGTGAGAACA ACATCACGGT CCCCTGTCCT GTCCCGCCGG 
    GATTCCCCAC TGCAGGGTAG TGGGCAGCAG AGTAACCAGG CTGGTCAGAG AAACTCCACC 
    AGTAGCATCG AGCCGAGGCT GCTCTGGGAG AGAGTGGAGA AGCTGGCGCC CAGGCCGGGC 
    AGTGGCAGCT CCTCGGGGTC CAGCAACTCG GGGTCCCAGC CCGGCTCCCA CCCAGGGTCT 
    CAGAGTGGCT CCGGGGAGCG CTTCAGAGTG AGATCATCAT CCAAATCTGA AGGCTCGCCA 
    TCTCAGCGCC TTGAAAACGC AGCAAAAAAG CCTGAAGATA AAAAGGAAGT GTTTAGACCT 
    CTCAAGCCTG CTGGTGAAGT GGACCTGACG GCTCTGGCCA AAGAGCTGCG GGCCGTGGAG 
    GATGTGCGGC CGCCGCACAA GGTGACAGAC TACTCCTCCT CCAGCGAGGA GTCGGGGACA 
    ACGGACGAGG AGGACGACGA CGTGGAGCAG GAGGGGGCCG AGGAGCCTAC GTCTGGCCCG 
    GAGGACACCA GAGCTGCATC ATCTCTGAAC TTGAGCAATG GTGAAACAGA GTCCGTGAAA 
    ACCATGGTTG TCCATGACGA CGTGGAGAGT GAACCAGCCC TGACCCCGTC CAAAGAGGGC 
    ACGCTGATCG TCCGCCAGAG TGCAGTTGAC CAAAAGCGCG CCAGCCATCA TGAGAGCAAT 
    GGCTTTGCGG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC 
    ACTTCCTCCA CCTCCTCTTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG 
    ACACCCCAGG ACAAGCTCAC TACTAATGAG ACTCAGTCCG CTAGTAACAC ACTCCAGAAA 
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
    TCTAGCGGGA CAACAGTGAC TTCTGTGGTG GGGTTTTCCT GTGATGGAAT GAGACCTGAG 
    GCCATGAGGC AAGATCCTAC GCGGAAGGGC TCAGTGGTCA ATGTCAATCC CACCAATACT 
    CGGCCCCAGA GCGACACCCC AGAGATTCGC AAATACAAGA AGAGGTTTAA CTCCGAGATC 
    CTGTGCGCAG CCTTGTGGGG AGTGAACTTG TTGGTGGGTA CCGAGAGTGG CCTGATGCTG 
    CTGGACAGAA GTGGCCAAGG GAAGGTGTAT CCTCTGATCA ACAGAAGGCG ATTTCAGCAG 
    ATGGACGTCC TGGAAGGCTT GAACGTCTTA GTGACGATAT CTGGCAAAAA GGATAAGTTA 
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAACGACCC GGAGGTTGAG 
    AAGAAGCAGG GCTGGACGAC AGTGGGGGAC CTGGAAGGCT GTGTGCATTA TAAAGTTGTA 
    AAATATGAAA GGATCAAATT TCTGGTCATT GCTTTGAAGA ACTCTGTGGA AGTCTACGCA 
    TGGGCACCCA AGCCATATCA CAAATTCATG GCCTTTAAGT CATTTGGTGA GCTGGTGCAC 
    AAGCCGTTGC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGCTGAAAGT GATCTATGGA 
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GACTCAGGAT CGGTCTATGA CATTTACCTA 
    CCAACACACA TCCAATGTAG CATCAAACCC CACGCAATCA TCATCCTCCC CAACACAGAT 
    GGCATGGAGC TCCTGGTGTG CTATGAAGAT GAAGGGGTTT ACGTGAACAC GTACGGGAGG 
    ATCACCAAGG ACGTGGTTCT GCAGTGGGGA GAGATGCCAA CGTCTGTAGC ATATATTCGA 
    TCCAATCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGATCTGT GGAAACTGGC 
    CACTTGGATG GCGTGTTTAT GCATAAAAGG GCTCAAAGGC TGAAGTTCTT GTGTGAACGT 
    AATGACAAGG TGTTCTTTGC CTCCGTCCGG TCTGGGGGCA GCAGCCAGGT TTACTTCATG 
    ACTCTCGGCA GGACCTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_010809817.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010808119.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), mRNA.
    Price & Availability↑
    CloneID OBa47360
    Clone ID Related Accession (Same CDS sequence) XM_015465311.1 , XM_010809817.2
    Accession Version XM_010809817.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104287.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010809817.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGTTCT GTGGGGCCGG ATCCATCACG GACCTGGTGA AGAACACCAA GGGCAACACG 
    CTAAAGGAAG ACTGGATAGC CTACATCTCC AGGGAGATCC TTCGGGGCCT GGCCCATCTC 
    CACATCCATC ACGTGATCCA CCGCGACATC AAGGGCCAGA ATGTGCTGCT GACCGAGAAC 
    GCAGAGGTGA AGCTGGTGGA CTTCGGGGTG AGTGCCCAGC TGGACAGGAC CGTGGGGAGG 
    AGGAACACGT TCATAGGCAC CCCGTACTGG ATGGCCCCTG AGGTCATTGC CTGTGACGAG 
    AACCCCGACG CCACCTACGA TTACAGGAGT GACCTCTGGT CCTGTGGCAT CACTGCCATT 
    GAGATGGCTG AAGGCGCTCC CCCTCTCTGC GACATGCATC CCATGAGAGC CCTGTTTCTC 
    ATCCCCCGGA ATCCTCCTCC CCGGCTGAAG TCCAAAAAAT GGTCCAAGAA GTTTTTTAGT 
    TTTATAGAAG GATGCCTGGT GAAGAATTAC ATGCAGCGGC CCTCCACCGA GCAGCTTCTG 
    AAGCATCCTT TCATTAGGGA TCAGCCGAAC GAAAGGCAGG TTAGAATCCA GCTTAAGGAC 
    CATATAGACC GGACCAGGAA GAAGAGAGGA GAGAAAGATG AAACCGAGTA TGAGTACAGT 
    GGGAGTGAGG AAGAGGAGGA GGAGGCGCCT GAGCAGGAGG GGGAGCCCAG CTCCATCGTC 
    AACGTGCCGG GGGAGTCGAC GCTGCGCCGG GACTTCCTGC GGCTCCAGCA GGAGAACAAG 
    GAGCGCTCTG AGGCCCTGAG GAGGCAGCAG CTCCTGCAGG AGCAGCAGCT CCGCGAGCAG 
    GAGGAGTATA AACGGCAGCT GCTGGCGGAG AGGCAGAAGC GGATCGAGCA GCAGAAGGAG 
    CAGAGGCGGC GGCTGGAAGA GCAACAGAGG AGAGAGCGGG AAGCCAGAAG GCAGCAGGAA 
    CGTGAGCAGC GGAGGCGGGA ACAGGAGGAG AAGAGGCGGC TGGAGGAGCT GGAGAGACGA 
    CGGAAGGAGG AGGAGGAGCG GAGGCGGGCG GAGGAGGAGA AGAGGAGAGT GGAGAGGGAG 
    CAGGAGTATA TCCGACGGCA GCTGGAAGAG GAACAGCGTC ACCTGGAACT GCTTCAGCAG 
    CAGCTGCTCC AGGAGCAGGC CATGCTACTG GAGTGTCGCT GGCGGGAGCT GGAGGAGCAC 
    CGGCAGGCAG AGCGGCTCCA GAGGCAGCTG CAGCAAGAAC AAGCCTATCT CCTGTCTCTA 
    CAGCACGACC CCCGGAGGCC GCCCCCGCCG CCAGACAGGA GCAAGGCTGG CCTGCACGAG 
    CCCAAGGCCC CGCACGAGCC CGCTGACCGG GCCCGTGAGG TGGAAGATAG ATTTAGGAAA 
    ACTAACCACA GCTCCCCCGA AGCCCAGTCT AAGCAGACGG GCCGAGTGTT GGAACCTCCA 
    GTGCCTTCCA GATCAGAGTC TTTTTCGAGT GGCAACTCCG AGTCTGTGCA CCCTGCCCTG 
    CAGAGACCCG CCGAGCCACA GGTACAGTGG TCCCACCTGG CATCTCTCAA GAACAATGTT 
    TCCCCTGTCT CGAGATCCCA TTCCTTCAGT GACCCTTCTC CTCCCAAATT TGCACACCAC 
    CATCTTCGCT CTCAGGACCC CTGCCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGTTCT 
    GACTCTAAGG CGGAGGTGCC CGACCCAGCC CAGAAGGCTT GGTCTAGATC AGACAGTGAC 
    GAGGTGCCTC CACGGGTCCC TGTGAGAACA ACATCACGGT CCCCTGTCCT GTCCCGCCGG 
    GATTCCCCAC TGCAGGGTAG TGGGCAGCAG AGTAACCAGG CTGGTCAGAG AAACTCCACC 
    AGTAGCATCG AGCCGAGGCT GCTCTGGGAG AGAGTGGAGA AGCTGGCGCC CAGGCCGGGC 
    AGTGGCAGCT CCTCGGGGTC CAGCAACTCG GGGTCCCAGC CCGGCTCCCA CCCAGGGTCT 
    CAGAGTGGCT CCGGGGAGCG CTTCAGAGTG AGATCATCAT CCAAATCTGA AGGCTCGCCA 
    TCTCAGCGCC TTGAAAACGC AGCAAAAAAG CCTGAAGATA AAAAGGAAGT GTTTAGACCT 
    CTCAAGCCTG CTGGTGAAGT GGACCTGACG GCTCTGGCCA AAGAGCTGCG GGCCGTGGAG 
    GATGTGCGGC CGCCGCACAA GGTGACAGAC TACTCCTCCT CCAGCGAGGA GTCGGGGACA 
    ACGGACGAGG AGGACGACGA CGTGGAGCAG GAGGGGGCCG AGGAGCCTAC GTCTGGCCCG 
    GAGGACACCA GAGCTGCATC ATCTCTGAAC TTGAGCAATG GTGAAACAGA GTCCGTGAAA 
    ACCATGGTTG TCCATGACGA CGTGGAGAGT GAACCAGCCC TGACCCCGTC CAAAGAGGGC 
    ACGCTGATCG TCCGCCAGAG TGCAGTTGAC CAAAAGCGCG CCAGCCATCA TGAGAGCAAT 
    GGCTTTGCGG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC 
    ACTTCCTCCA CCTCCTCTTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG 
    ACACCCCAGG ACAAGCTCAC TACTAATGAG ACTCAGTCCG CTAGTAACAC ACTCCAGAAA 
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
    TCTAGCGGGA CAACAGTGAC TTCTGTGGTG GGGTTTTCCT GTGATGGAAT GAGACCTGAG 
    GCCATGAGGC AAGATCCTAC GCGGAAGGGC TCAGTGGTCA ATGTCAATCC CACCAATACT 
    CGGCCCCAGA GCGACACCCC AGAGATTCGC AAATACAAGA AGAGGTTTAA CTCCGAGATC 
    CTGTGCGCAG CCTTGTGGGG AGTGAACTTG TTGGTGGGTA CCGAGAGTGG CCTGATGCTG 
    CTGGACAGAA GTGGCCAAGG GAAGGTGTAT CCTCTGATCA ACAGAAGGCG ATTTCAGCAG 
    ATGGACGTCC TGGAAGGCTT GAACGTCTTA GTGACGATAT CTGGCAAAAA GGATAAGTTA 
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAACGACCC GGAGGTTGAG 
    AAGAAGCAGG GCTGGACGAC AGTGGGGGAC CTGGAAGGCT GTGTGCATTA TAAAGTTGTA 
    AAATATGAAA GGATCAAATT TCTGGTCATT GCTTTGAAGA ACTCTGTGGA AGTCTACGCA 
    TGGGCACCCA AGCCATATCA CAAATTCATG GCCTTTAAGT CATTTGGTGA GCTGGTGCAC 
    AAGCCGTTGC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGCTGAAAGT GATCTATGGA 
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GACTCAGGAT CGGTCTATGA CATTTACCTA 
    CCAACACACA TCCAATGTAG CATCAAACCC CACGCAATCA TCATCCTCCC CAACACAGAT 
    GGCATGGAGC TCCTGGTGTG CTATGAAGAT GAAGGGGTTT ACGTGAACAC GTACGGGAGG 
    ATCACCAAGG ACGTGGTTCT GCAGTGGGGA GAGATGCCAA CGTCTGTAGC ATATATTCGA 
    TCCAATCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGATCTGT GGAAACTGGC 
    CACTTGGATG GCGTGTTTAT GCATAAAAGG GCTCAAAGGC TGAAGTTCTT GTGTGAACGT 
    AATGACAAGG TGTTCTTTGC CTCCGTCCGG TCTGGGGGCA GCAGCCAGGT TTACTTCATG 
    ACTCTCGGCA GGACCTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010808119.1
    CDS34..3846
    Translation

    Target ORF information:

    RefSeq Version XM_010809817.2
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010809817.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGTTCT GTGGGGCCGG ATCCATCACG GACCTGGTGA AGAACACCAA GGGCAACACG 
    CTAAAGGAAG ACTGGATAGC CTACATCTCC AGGGAGATCC TTCGGGGCCT GGCCCATCTC 
    CACATCCATC ACGTGATCCA CCGCGACATC AAGGGCCAGA ATGTGCTGCT GACCGAGAAC 
    GCAGAGGTGA AGCTGGTGGA CTTCGGGGTG AGTGCCCAGC TGGACAGGAC CGTGGGGAGG 
    AGGAACACGT TCATAGGCAC CCCGTACTGG ATGGCCCCTG AGGTCATTGC CTGTGACGAG 
    AACCCCGACG CCACCTACGA TTACAGGAGT GACCTCTGGT CCTGTGGCAT CACTGCCATT 
    GAGATGGCTG AAGGCGCTCC CCCTCTCTGC GACATGCATC CCATGAGAGC CCTGTTTCTC 
    ATCCCCCGGA ATCCTCCTCC CCGGCTGAAG TCCAAAAAAT GGTCCAAGAA GTTTTTTAGT 
    TTTATAGAAG GATGCCTGGT GAAGAATTAC ATGCAGCGGC CCTCCACCGA GCAGCTTCTG 
    AAGCATCCTT TCATTAGGGA TCAGCCGAAC GAAAGGCAGG TTAGAATCCA GCTTAAGGAC 
    CATATAGACC GGACCAGGAA GAAGAGAGGA GAGAAAGATG AAACCGAGTA TGAGTACAGT 
    GGGAGTGAGG AAGAGGAGGA GGAGGCGCCT GAGCAGGAGG GGGAGCCCAG CTCCATCGTC 
    AACGTGCCGG GGGAGTCGAC GCTGCGCCGG GACTTCCTGC GGCTCCAGCA GGAGAACAAG 
    GAGCGCTCTG AGGCCCTGAG GAGGCAGCAG CTCCTGCAGG AGCAGCAGCT CCGCGAGCAG 
    GAGGAGTATA AACGGCAGCT GCTGGCGGAG AGGCAGAAGC GGATCGAGCA GCAGAAGGAG 
    CAGAGGCGGC GGCTGGAAGA GCAACAGAGG AGAGAGCGGG AAGCCAGAAG GCAGCAGGAA 
    CGTGAGCAGC GGAGGCGGGA ACAGGAGGAG AAGAGGCGGC TGGAGGAGCT GGAGAGACGA 
    CGGAAGGAGG AGGAGGAGCG GAGGCGGGCG GAGGAGGAGA AGAGGAGAGT GGAGAGGGAG 
    CAGGAGTATA TCCGACGGCA GCTGGAAGAG GAACAGCGTC ACCTGGAACT GCTTCAGCAG 
    CAGCTGCTCC AGGAGCAGGC CATGCTACTG GAGTGTCGCT GGCGGGAGCT GGAGGAGCAC 
    CGGCAGGCAG AGCGGCTCCA GAGGCAGCTG CAGCAAGAAC AAGCCTATCT CCTGTCTCTA 
    CAGCACGACC CCCGGAGGCC GCCCCCGCCG CCAGACAGGA GCAAGGCTGG CCTGCACGAG 
    CCCAAGGCCC CGCACGAGCC CGCTGACCGG GCCCGTGAGG TGGAAGATAG ATTTAGGAAA 
    ACTAACCACA GCTCCCCCGA AGCCCAGTCT AAGCAGACGG GCCGAGTGTT GGAACCTCCA 
    GTGCCTTCCA GATCAGAGTC TTTTTCGAGT GGCAACTCCG AGTCTGTGCA CCCTGCCCTG 
    CAGAGACCCG CCGAGCCACA GGTACAGTGG TCCCACCTGG CATCTCTCAA GAACAATGTT 
    TCCCCTGTCT CGAGATCCCA TTCCTTCAGT GACCCTTCTC CTCCCAAATT TGCACACCAC 
    CATCTTCGCT CTCAGGACCC CTGCCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGTTCT 
    GACTCTAAGG CGGAGGTGCC CGACCCAGCC CAGAAGGCTT GGTCTAGATC AGACAGTGAC 
    GAGGTGCCTC CACGGGTCCC TGTGAGAACA ACATCACGGT CCCCTGTCCT GTCCCGCCGG 
    GATTCCCCAC TGCAGGGTAG TGGGCAGCAG AGTAACCAGG CTGGTCAGAG AAACTCCACC 
    AGTAGCATCG AGCCGAGGCT GCTCTGGGAG AGAGTGGAGA AGCTGGCGCC CAGGCCGGGC 
    AGTGGCAGCT CCTCGGGGTC CAGCAACTCG GGGTCCCAGC CCGGCTCCCA CCCAGGGTCT 
    CAGAGTGGCT CCGGGGAGCG CTTCAGAGTG AGATCATCAT CCAAATCTGA AGGCTCGCCA 
    TCTCAGCGCC TTGAAAACGC AGCAAAAAAG CCTGAAGATA AAAAGGAAGT GTTTAGACCT 
    CTCAAGCCTG CTGGTGAAGT GGACCTGACG GCTCTGGCCA AAGAGCTGCG GGCCGTGGAG 
    GATGTGCGGC CGCCGCACAA GGTGACAGAC TACTCCTCCT CCAGCGAGGA GTCGGGGACA 
    ACGGACGAGG AGGACGACGA CGTGGAGCAG GAGGGGGCCG AGGAGCCTAC GTCTGGCCCG 
    GAGGACACCA GAGCTGCATC ATCTCTGAAC TTGAGCAATG GTGAAACAGA GTCCGTGAAA 
    ACCATGGTTG TCCATGACGA CGTGGAGAGT GAACCAGCCC TGACCCCGTC CAAAGAGGGC 
    ACGCTGATCG TCCGCCAGAG TGCAGTTGAC CAAAAGCGCG CCAGCCATCA TGAGAGCAAT 
    GGCTTTGCGG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC 
    ACTTCCTCCA CCTCCTCTTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG 
    ACACCCCAGG ACAAGCTCAC TACTAATGAG ACTCAGTCCG CTAGTAACAC ACTCCAGAAA 
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
    TCTAGCGGGA CAACAGTGAC TTCTGTGGTG GGGTTTTCCT GTGATGGAAT GAGACCTGAG 
    GCCATGAGGC AAGATCCTAC GCGGAAGGGC TCAGTGGTCA ATGTCAATCC CACCAATACT 
    CGGCCCCAGA GCGACACCCC AGAGATTCGC AAATACAAGA AGAGGTTTAA CTCCGAGATC 
    CTGTGCGCAG CCTTGTGGGG AGTGAACTTG TTGGTGGGTA CCGAGAGTGG CCTGATGCTG 
    CTGGACAGAA GTGGCCAAGG GAAGGTGTAT CCTCTGATCA ACAGAAGGCG ATTTCAGCAG 
    ATGGACGTCC TGGAAGGCTT GAACGTCTTA GTGACGATAT CTGGCAAAAA GGATAAGTTA 
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAACGACCC GGAGGTTGAG 
    AAGAAGCAGG GCTGGACGAC AGTGGGGGAC CTGGAAGGCT GTGTGCATTA TAAAGTTGTA 
    AAATATGAAA GGATCAAATT TCTGGTCATT GCTTTGAAGA ACTCTGTGGA AGTCTACGCA 
    TGGGCACCCA AGCCATATCA CAAATTCATG GCCTTTAAGT CATTTGGTGA GCTGGTGCAC 
    AAGCCGTTGC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGCTGAAAGT GATCTATGGA 
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GACTCAGGAT CGGTCTATGA CATTTACCTA 
    CCAACACACA TCCAATGTAG CATCAAACCC CACGCAATCA TCATCCTCCC CAACACAGAT 
    GGCATGGAGC TCCTGGTGTG CTATGAAGAT GAAGGGGTTT ACGTGAACAC GTACGGGAGG 
    ATCACCAAGG ACGTGGTTCT GCAGTGGGGA GAGATGCCAA CGTCTGTAGC ATATATTCGA 
    TCCAATCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGATCTGT GGAAACTGGC 
    CACTTGGATG GCGTGTTTAT GCATAAAAGG GCTCAAAGGC TGAAGTTCTT GTGTGAACGT 
    AATGACAAGG TGTTCTTTGC CTCCGTCCGG TCTGGGGGCA GCAGCCAGGT TTACTTCATG 
    ACTCTCGGCA GGACCTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999322.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855090.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OBa220746
    Clone ID Related Accession (Same CDS sequence) XM_024999322.1
    Accession Version XM_024999322.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCACGGGTC CCTGTGAGAA CAACATCACG GTCCCCTGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGT AGTGGGCAGC AGAGTAACCA GGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCGAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCGG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CGGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCTCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGATGTGCG GCCGCCGCAC AAGGTGACAG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CAACGGACGA GGAGGACGAC GACGTGGAGC AGGAGGGGGC CGAGGAGCCT 
    ACGTCTGGCC CGGAGGACAC CAGAGCTGCA TCATCTCTGA ACTTGAGCAA TGGTGAAACA 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT 
    CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
    CATTCCTCCT CCACTTCCTC CACCTCCTCT TCCCCATCCT CCAGCCAGCC GACACCCACC 
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTACTAATG AGACTCAGTC CGCTAGTAAC 
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
    CAGATTTCTC CATCTAGCGG GACAACAGTG ACTTCTGTGG TGGGGTTTTC CTGTGATGGA 
    ATGAGACCTG AGGCCATGAG GCAAGATCCT ACGCGGAAGG GCTCAGTGGT CAATGTCAAT 
    CCCACCAATA CTCGGCCCCA GAGCGACACC CCAGAGATTC GCAAATACAA GAAGAGGTTT 
    AACTCCGAGA TCCTGTGCGC AGCCTTGTGG GGAGTGAACT TGTTGGTGGG TACCGAGAGT 
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTGT ATCCTCTGAT CAACAGAAGG 
    CGATTTCAGC AGATGGACGT CCTGGAAGGC TTGAACGTCT TAGTGACGAT ATCTGGCAAA 
    AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAACGAC 
    CCGGAGGTTG AGAAGAAGCA GGGCTGGACG ACAGTGGGGG ACCTGGAAGG CTGTGTGCAT 
    TATAAAGTTG TAAAATATGA AAGGATCAAA TTTCTGGTCA TTGCTTTGAA GAACTCTGTG 
    GAAGTCTACG CATGGGCACC CAAGCCATAT CACAAATTCA TGGCCTTTAA GTCATTTGGT 
    GAGCTGGTGC ACAAGCCGTT GCTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGCTGAAA 
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGACTCAGG ATCGGTCTAT 
    GACATTTACC TACCAACACA CGTAAGAAAG AACCCACACT CTATGATCCA ATGTAGCATC 
    AAACCCCACG CAATCATCAT CCTCCCCAAC ACAGATGGCA TGGAGCTCCT GGTGTGCTAT 
    GAAGATGAAG GGGTTTACGT GAACACGTAC GGGAGGATCA CCAAGGACGT GGTTCTGCAG 
    TGGGGAGAGA TGCCAACGTC TGTAGCATAT ATTCGATCCA ATCAGACGAT GGGCTGGGGA 
    GAAAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGCCACT TGGATGGCGT GTTTATGCAT 
    AAAAGGGCTC AAAGGCTGAA GTTCTTGTGT GAACGTAATG ACAAGGTGTT CTTTGCCTCC 
    GTCCGGTCTG GGGGCAGCAG CCAGGTTTAC TTCATGACTC TCGGCAGGAC CTCTCTTCTG 
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855090.1
    CDS122..4270
    Translation

    Target ORF information:

    RefSeq Version XM_024999322.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999322.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCACGGGTC CCTGTGAGAA CAACATCACG GTCCCCTGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGT AGTGGGCAGC AGAGTAACCA GGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCGAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCGG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CGGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCTCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGATGTGCG GCCGCCGCAC AAGGTGACAG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CAACGGACGA GGAGGACGAC GACGTGGAGC AGGAGGGGGC CGAGGAGCCT 
    ACGTCTGGCC CGGAGGACAC CAGAGCTGCA TCATCTCTGA ACTTGAGCAA TGGTGAAACA 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT 
    CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
    CATTCCTCCT CCACTTCCTC CACCTCCTCT TCCCCATCCT CCAGCCAGCC GACACCCACC 
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTACTAATG AGACTCAGTC CGCTAGTAAC 
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
    CAGATTTCTC CATCTAGCGG GACAACAGTG ACTTCTGTGG TGGGGTTTTC CTGTGATGGA 
    ATGAGACCTG AGGCCATGAG GCAAGATCCT ACGCGGAAGG GCTCAGTGGT CAATGTCAAT 
    CCCACCAATA CTCGGCCCCA GAGCGACACC CCAGAGATTC GCAAATACAA GAAGAGGTTT 
    AACTCCGAGA TCCTGTGCGC AGCCTTGTGG GGAGTGAACT TGTTGGTGGG TACCGAGAGT 
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTGT ATCCTCTGAT CAACAGAAGG 
    CGATTTCAGC AGATGGACGT CCTGGAAGGC TTGAACGTCT TAGTGACGAT ATCTGGCAAA 
    AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAACGAC 
    CCGGAGGTTG AGAAGAAGCA GGGCTGGACG ACAGTGGGGG ACCTGGAAGG CTGTGTGCAT 
    TATAAAGTTG TAAAATATGA AAGGATCAAA TTTCTGGTCA TTGCTTTGAA GAACTCTGTG 
    GAAGTCTACG CATGGGCACC CAAGCCATAT CACAAATTCA TGGCCTTTAA GTCATTTGGT 
    GAGCTGGTGC ACAAGCCGTT GCTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGCTGAAA 
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGACTCAGG ATCGGTCTAT 
    GACATTTACC TACCAACACA CGTAAGAAAG AACCCACACT CTATGATCCA ATGTAGCATC 
    AAACCCCACG CAATCATCAT CCTCCCCAAC ACAGATGGCA TGGAGCTCCT GGTGTGCTAT 
    GAAGATGAAG GGGTTTACGT GAACACGTAC GGGAGGATCA CCAAGGACGT GGTTCTGCAG 
    TGGGGAGAGA TGCCAACGTC TGTAGCATAT ATTCGATCCA ATCAGACGAT GGGCTGGGGA 
    GAAAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGCCACT TGGATGGCGT GTTTATGCAT 
    AAAAGGGCTC AAAGGCTGAA GTTCTTGTGT GAACGTAATG ACAAGGTGTT CTTTGCCTCC 
    GTCCGGTCTG GGGGCAGCAG CCAGGTTTAC TTCATGACTC TCGGCAGGAC CTCTCTTCTG 
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999323.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855091.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OBa220747
    Clone ID Related Accession (Same CDS sequence) XM_024999323.1
    Accession Version XM_024999323.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCACGGGTC CCTGTGAGAA CAACATCACG GTCCCCTGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGT AGTGGGCAGC AGAGTAACCA GGCTGGTCAG 
    AGAAACTCCA CCAGCATCGA GCCGAGGCTG CTCTGGGAGA GAGTGGAGAA GCTGGCGCCC 
    AGGCCGGGCA GTGGCAGCTC CTCGGGGTCC AGCAACTCGG GGTCCCAGCC CGGCTCCCAC 
    CCAGGGTCTC AGAGTGGCTC CGGGGAGCGC TTCAGAGTGA GATCATCATC CAAATCTGAA 
    GGCTCGCCAT CTCAGCGCCT TGAAAACGCA GCAAAAAAGC CTGAAGATAA AAAGGAAGTG 
    TTTAGACCTC TCAAGCCTGC TGGTGAAGTG GACCTGACGG CTCTGGCCAA AGAGCTGCGG 
    GCCGTGGAGG ATGTGCGGCC GCCGCACAAG GTGACAGACT ACTCCTCCTC CAGCGAGGAG 
    TCGGGGACAA CGGACGAGGA GGACGACGAC GTGGAGCAGG AGGGGGCCGA GGAGCCTACG 
    TCTGGCCCGG AGGACACCAG AGCTGCATCA TCTCTGAACT TGAGCAATGG TGAAACAGAG 
    TCCGTGAAAA CCATGGTTGT CCATGACGAC GTGGAGAGTG AACCAGCCCT GACCCCGTCC 
    AAAGAGGGCA CGCTGATCGT CCGCCAGAGT GCAGTTGACC AAAAGCGCGC CAGCCATCAT 
    GAGAGCAATG GCTTTGCGGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
    TCCTCCTCCA CTTCCTCCAC CTCCTCTTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
    TCCCCACAGA CACCCCAGGA CAAGCTCACT ACTAATGAGA CTCAGTCCGC TAGTAACACA 
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
    ATTTCTCCAT CTAGCGGGAC AACAGTGACT TCTGTGGTGG GGTTTTCCTG TGATGGAATG 
    AGACCTGAGG CCATGAGGCA AGATCCTACG CGGAAGGGCT CAGTGGTCAA TGTCAATCCC 
    ACCAATACTC GGCCCCAGAG CGACACCCCA GAGATTCGCA AATACAAGAA GAGGTTTAAC 
    TCCGAGATCC TGTGCGCAGC CTTGTGGGGA GTGAACTTGT TGGTGGGTAC CGAGAGTGGC 
    CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTGTATC CTCTGATCAA CAGAAGGCGA 
    TTTCAGCAGA TGGACGTCCT GGAAGGCTTG AACGTCTTAG TGACGATATC TGGCAAAAAG 
    GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAACGACCCG 
    GAGGTTGAGA AGAAGCAGGG CTGGACGACA GTGGGGGACC TGGAAGGCTG TGTGCATTAT 
    AAAGTTGTAA AATATGAAAG GATCAAATTT CTGGTCATTG CTTTGAAGAA CTCTGTGGAA 
    GTCTACGCAT GGGCACCCAA GCCATATCAC AAATTCATGG CCTTTAAGTC ATTTGGTGAG 
    CTGGTGCACA AGCCGTTGCT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GCTGAAAGTG 
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ACTCAGGATC GGTCTATGAC 
    ATTTACCTAC CAACACACGT AAGAAAGAAC CCACACTCTA TGATCCAATG TAGCATCAAA 
    CCCCACGCAA TCATCATCCT CCCCAACACA GATGGCATGG AGCTCCTGGT GTGCTATGAA 
    GATGAAGGGG TTTACGTGAA CACGTACGGG AGGATCACCA AGGACGTGGT TCTGCAGTGG 
    GGAGAGATGC CAACGTCTGT AGCATATATT CGATCCAATC AGACGATGGG CTGGGGAGAA 
    AAGGCCATAG AGATCCGATC TGTGGAAACT GGCCACTTGG ATGGCGTGTT TATGCATAAA 
    AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA CGTAATGACA AGGTGTTCTT TGCCTCCGTC 
    CGGTCTGGGG GCAGCAGCCA GGTTTACTTC ATGACTCTCG GCAGGACCTC TCTTCTGAGC 
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855091.1
    CDS122..4267
    Translation

    Target ORF information:

    RefSeq Version XM_024999323.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999323.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCACGGGTC CCTGTGAGAA CAACATCACG GTCCCCTGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGT AGTGGGCAGC AGAGTAACCA GGCTGGTCAG 
    AGAAACTCCA CCAGCATCGA GCCGAGGCTG CTCTGGGAGA GAGTGGAGAA GCTGGCGCCC 
    AGGCCGGGCA GTGGCAGCTC CTCGGGGTCC AGCAACTCGG GGTCCCAGCC CGGCTCCCAC 
    CCAGGGTCTC AGAGTGGCTC CGGGGAGCGC TTCAGAGTGA GATCATCATC CAAATCTGAA 
    GGCTCGCCAT CTCAGCGCCT TGAAAACGCA GCAAAAAAGC CTGAAGATAA AAAGGAAGTG 
    TTTAGACCTC TCAAGCCTGC TGGTGAAGTG GACCTGACGG CTCTGGCCAA AGAGCTGCGG 
    GCCGTGGAGG ATGTGCGGCC GCCGCACAAG GTGACAGACT ACTCCTCCTC CAGCGAGGAG 
    TCGGGGACAA CGGACGAGGA GGACGACGAC GTGGAGCAGG AGGGGGCCGA GGAGCCTACG 
    TCTGGCCCGG AGGACACCAG AGCTGCATCA TCTCTGAACT TGAGCAATGG TGAAACAGAG 
    TCCGTGAAAA CCATGGTTGT CCATGACGAC GTGGAGAGTG AACCAGCCCT GACCCCGTCC 
    AAAGAGGGCA CGCTGATCGT CCGCCAGAGT GCAGTTGACC AAAAGCGCGC CAGCCATCAT 
    GAGAGCAATG GCTTTGCGGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
    TCCTCCTCCA CTTCCTCCAC CTCCTCTTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
    TCCCCACAGA CACCCCAGGA CAAGCTCACT ACTAATGAGA CTCAGTCCGC TAGTAACACA 
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
    ATTTCTCCAT CTAGCGGGAC AACAGTGACT TCTGTGGTGG GGTTTTCCTG TGATGGAATG 
    AGACCTGAGG CCATGAGGCA AGATCCTACG CGGAAGGGCT CAGTGGTCAA TGTCAATCCC 
    ACCAATACTC GGCCCCAGAG CGACACCCCA GAGATTCGCA AATACAAGAA GAGGTTTAAC 
    TCCGAGATCC TGTGCGCAGC CTTGTGGGGA GTGAACTTGT TGGTGGGTAC CGAGAGTGGC 
    CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTGTATC CTCTGATCAA CAGAAGGCGA 
    TTTCAGCAGA TGGACGTCCT GGAAGGCTTG AACGTCTTAG TGACGATATC TGGCAAAAAG 
    GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAACGACCCG 
    GAGGTTGAGA AGAAGCAGGG CTGGACGACA GTGGGGGACC TGGAAGGCTG TGTGCATTAT 
    AAAGTTGTAA AATATGAAAG GATCAAATTT CTGGTCATTG CTTTGAAGAA CTCTGTGGAA 
    GTCTACGCAT GGGCACCCAA GCCATATCAC AAATTCATGG CCTTTAAGTC ATTTGGTGAG 
    CTGGTGCACA AGCCGTTGCT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GCTGAAAGTG 
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ACTCAGGATC GGTCTATGAC 
    ATTTACCTAC CAACACACGT AAGAAAGAAC CCACACTCTA TGATCCAATG TAGCATCAAA 
    CCCCACGCAA TCATCATCCT CCCCAACACA GATGGCATGG AGCTCCTGGT GTGCTATGAA 
    GATGAAGGGG TTTACGTGAA CACGTACGGG AGGATCACCA AGGACGTGGT TCTGCAGTGG 
    GGAGAGATGC CAACGTCTGT AGCATATATT CGATCCAATC AGACGATGGG CTGGGGAGAA 
    AAGGCCATAG AGATCCGATC TGTGGAAACT GGCCACTTGG ATGGCGTGTT TATGCATAAA 
    AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA CGTAATGACA AGGTGTTCTT TGCCTCCGTC 
    CGGTCTGGGG GCAGCAGCCA GGTTTACTTC ATGACTCTCG GCAGGACCTC TCTTCTGAGC 
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999324.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855092.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OBa220748
    Clone ID Related Accession (Same CDS sequence) XM_024999324.1
    Accession Version XM_024999324.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGTGGTCCC ACCTGGCATC TCTCAAGAAC 
    AATGTTTCCC CTGTCTCGAG ATCCCATTCC TTCAGTGACC CTTCTCCTCC CAAATTTGCA 
    CACCACCATC TTCGCTCTCA GGACCCCTGC CCACCTTCCC GCAGTGAGGT GCTCAGTCAG 
    AGTTCTGACT CTAAGGCGGA GGTGCCCGAC CCAGCCCAGA AGGCTTGGTC TAGATCAGAC 
    AGTGACGAGG TGCCTCCACG GGTCCCTGTG AGAACAACAT CACGGTCCCC TGTCCTGTCC 
    CGCCGGGATT CCCCACTGCA GGGTAGTGGG CAGCAGAGTA ACCAGGCTGG TCAGAGAAAC 
    TCCACCAGTA GCATCGAGCC GAGGCTGCTC TGGGAGAGAG TGGAGAAGCT GGCGCCCAGG 
    CCGGGCAGTG GCAGCTCCTC GGGGTCCAGC AACTCGGGGT CCCAGCCCGG CTCCCACCCA 
    GGGTCTCAGA GTGGCTCCGG GGAGCGCTTC AGAGTGAGAT CATCATCCAA ATCTGAAGGC 
    TCGCCATCTC AGCGCCTTGA AAACGCAGCA AAAAAGCCTG AAGATAAAAA GGAAGTGTTT 
    AGACCTCTCA AGCCTGCTGG TGAAGTGGAC CTGACGGCTC TGGCCAAAGA GCTGCGGGCC 
    GTGGAGGATG TGCGGCCGCC GCACAAGGTG ACAGACTACT CCTCCTCCAG CGAGGAGTCG 
    GGGACAACGG ACGAGGAGGA CGACGACGTG GAGCAGGAGG GGGCCGAGGA GCCTACGTCT 
    GGCCCGGAGG ACACCAGAGC TGCATCATCT CTGAACTTGA GCAATGGTGA AACAGAGTCC 
    GTGAAAACCA TGGTTGTCCA TGACGACGTG GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA 
    GAGGGCACGC TGATCGTCCG CCAGAGTGCA GTTGACCAAA AGCGCGCCAG CCATCATGAG 
    AGCAATGGCT TTGCGGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC 
    TCCTCCACTT CCTCCACCTC CTCTTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC 
    CCACAGACAC CCCAGGACAA GCTCACTACT AATGAGACTC AGTCCGCTAG TAACACACTC 
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
    TCTCCATCTA GCGGGACAAC AGTGACTTCT GTGGTGGGGT TTTCCTGTGA TGGAATGAGA 
    CCTGAGGCCA TGAGGCAAGA TCCTACGCGG AAGGGCTCAG TGGTCAATGT CAATCCCACC 
    AATACTCGGC CCCAGAGCGA CACCCCAGAG ATTCGCAAAT ACAAGAAGAG GTTTAACTCC 
    GAGATCCTGT GCGCAGCCTT GTGGGGAGTG AACTTGTTGG TGGGTACCGA GAGTGGCCTG 
    ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTGTATCCTC TGATCAACAG AAGGCGATTT 
    CAGCAGATGG ACGTCCTGGA AGGCTTGAAC GTCTTAGTGA CGATATCTGG CAAAAAGGAT 
    AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA CGACCCGGAG 
    GTTGAGAAGA AGCAGGGCTG GACGACAGTG GGGGACCTGG AAGGCTGTGT GCATTATAAA 
    GTTGTAAAAT ATGAAAGGAT CAAATTTCTG GTCATTGCTT TGAAGAACTC TGTGGAAGTC 
    TACGCATGGG CACCCAAGCC ATATCACAAA TTCATGGCCT TTAAGTCATT TGGTGAGCTG 
    GTGCACAAGC CGTTGCTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGCT GAAAGTGATC 
    TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGACT CAGGATCGGT CTATGACATT 
    TACCTACCAA CACACGTAAG AAAGAACCCA CACTCTATGA TCCAATGTAG CATCAAACCC 
    CACGCAATCA TCATCCTCCC CAACACAGAT GGCATGGAGC TCCTGGTGTG CTATGAAGAT 
    GAAGGGGTTT ACGTGAACAC GTACGGGAGG ATCACCAAGG ACGTGGTTCT GCAGTGGGGA 
    GAGATGCCAA CGTCTGTAGC ATATATTCGA TCCAATCAGA CGATGGGCTG GGGAGAAAAG 
    GCCATAGAGA TCCGATCTGT GGAAACTGGC CACTTGGATG GCGTGTTTAT GCATAAAAGG 
    GCTCAAAGGC TGAAGTTCTT GTGTGAACGT AATGACAAGG TGTTCTTTGC CTCCGTCCGG 
    TCTGGGGGCA GCAGCCAGGT TTACTTCATG ACTCTCGGCA GGACCTCTCT TCTGAGCTGG 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855092.1
    CDS122..4264
    Translation

    Target ORF information:

    RefSeq Version XM_024999324.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999324.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGTGGTCCC ACCTGGCATC TCTCAAGAAC 
    AATGTTTCCC CTGTCTCGAG ATCCCATTCC TTCAGTGACC CTTCTCCTCC CAAATTTGCA 
    CACCACCATC TTCGCTCTCA GGACCCCTGC CCACCTTCCC GCAGTGAGGT GCTCAGTCAG 
    AGTTCTGACT CTAAGGCGGA GGTGCCCGAC CCAGCCCAGA AGGCTTGGTC TAGATCAGAC 
    AGTGACGAGG TGCCTCCACG GGTCCCTGTG AGAACAACAT CACGGTCCCC TGTCCTGTCC 
    CGCCGGGATT CCCCACTGCA GGGTAGTGGG CAGCAGAGTA ACCAGGCTGG TCAGAGAAAC 
    TCCACCAGTA GCATCGAGCC GAGGCTGCTC TGGGAGAGAG TGGAGAAGCT GGCGCCCAGG 
    CCGGGCAGTG GCAGCTCCTC GGGGTCCAGC AACTCGGGGT CCCAGCCCGG CTCCCACCCA 
    GGGTCTCAGA GTGGCTCCGG GGAGCGCTTC AGAGTGAGAT CATCATCCAA ATCTGAAGGC 
    TCGCCATCTC AGCGCCTTGA AAACGCAGCA AAAAAGCCTG AAGATAAAAA GGAAGTGTTT 
    AGACCTCTCA AGCCTGCTGG TGAAGTGGAC CTGACGGCTC TGGCCAAAGA GCTGCGGGCC 
    GTGGAGGATG TGCGGCCGCC GCACAAGGTG ACAGACTACT CCTCCTCCAG CGAGGAGTCG 
    GGGACAACGG ACGAGGAGGA CGACGACGTG GAGCAGGAGG GGGCCGAGGA GCCTACGTCT 
    GGCCCGGAGG ACACCAGAGC TGCATCATCT CTGAACTTGA GCAATGGTGA AACAGAGTCC 
    GTGAAAACCA TGGTTGTCCA TGACGACGTG GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA 
    GAGGGCACGC TGATCGTCCG CCAGAGTGCA GTTGACCAAA AGCGCGCCAG CCATCATGAG 
    AGCAATGGCT TTGCGGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC 
    TCCTCCACTT CCTCCACCTC CTCTTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC 
    CCACAGACAC CCCAGGACAA GCTCACTACT AATGAGACTC AGTCCGCTAG TAACACACTC 
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
    TCTCCATCTA GCGGGACAAC AGTGACTTCT GTGGTGGGGT TTTCCTGTGA TGGAATGAGA 
    CCTGAGGCCA TGAGGCAAGA TCCTACGCGG AAGGGCTCAG TGGTCAATGT CAATCCCACC 
    AATACTCGGC CCCAGAGCGA CACCCCAGAG ATTCGCAAAT ACAAGAAGAG GTTTAACTCC 
    GAGATCCTGT GCGCAGCCTT GTGGGGAGTG AACTTGTTGG TGGGTACCGA GAGTGGCCTG 
    ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTGTATCCTC TGATCAACAG AAGGCGATTT 
    CAGCAGATGG ACGTCCTGGA AGGCTTGAAC GTCTTAGTGA CGATATCTGG CAAAAAGGAT 
    AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA CGACCCGGAG 
    GTTGAGAAGA AGCAGGGCTG GACGACAGTG GGGGACCTGG AAGGCTGTGT GCATTATAAA 
    GTTGTAAAAT ATGAAAGGAT CAAATTTCTG GTCATTGCTT TGAAGAACTC TGTGGAAGTC 
    TACGCATGGG CACCCAAGCC ATATCACAAA TTCATGGCCT TTAAGTCATT TGGTGAGCTG 
    GTGCACAAGC CGTTGCTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGCT GAAAGTGATC 
    TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGACT CAGGATCGGT CTATGACATT 
    TACCTACCAA CACACGTAAG AAAGAACCCA CACTCTATGA TCCAATGTAG CATCAAACCC 
    CACGCAATCA TCATCCTCCC CAACACAGAT GGCATGGAGC TCCTGGTGTG CTATGAAGAT 
    GAAGGGGTTT ACGTGAACAC GTACGGGAGG ATCACCAAGG ACGTGGTTCT GCAGTGGGGA 
    GAGATGCCAA CGTCTGTAGC ATATATTCGA TCCAATCAGA CGATGGGCTG GGGAGAAAAG 
    GCCATAGAGA TCCGATCTGT GGAAACTGGC CACTTGGATG GCGTGTTTAT GCATAAAAGG 
    GCTCAAAGGC TGAAGTTCTT GTGTGAACGT AATGACAAGG TGTTCTTTGC CTCCGTCCGG 
    TCTGGGGGCA GCAGCCAGGT TTACTTCATG ACTCTCGGCA GGACCTCTCT TCTGAGCTGG 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999325.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855093.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OBa220749
    Clone ID Related Accession (Same CDS sequence) XM_024999325.1
    Accession Version XM_024999325.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4140bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCACGGGTC CCTGTGAGAA CAACATCACG GTCCCCTGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGT AGTGGGCAGC AGAGTAACCA GGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCGAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCGG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CGGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGACCTG ACGGCTCTGG CCAAAGAGCT GCGGGCCGTG 
    GAGGATGTGC GGCCGCCGCA CAAGGTGACA GACTACTCCT CCTCCAGCGA GGAGTCGGGG 
    ACAACGGACG AGGAGGACGA CGACGTGGAG CAGGAGGGGG CCGAGGAGCC TACGTCTGGC 
    CCGGAGGACA CCAGAGCTGC ATCATCTCTG AACTTGAGCA ATGGTGAAAC AGAGTCCGTG 
    AAAACCATGG TTGTCCATGA CGACGTGGAG AGTGAACCAG CCCTGACCCC GTCCAAAGAG 
    GGCACGCTGA TCGTCCGCCA GAGTGCAGTT GACCAAAAGC GCGCCAGCCA TCATGAGAGC 
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
    TCCACTTCCT CCACCTCCTC TTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
    CAGACACCCC AGGACAAGCT CACTACTAAT GAGACTCAGT CCGCTAGTAA CACACTCCAG 
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
    CCATCTAGCG GGACAACAGT GACTTCTGTG GTGGGGTTTT CCTGTGATGG AATGAGACCT 
    GAGGCCATGA GGCAAGATCC TACGCGGAAG GGCTCAGTGG TCAATGTCAA TCCCACCAAT 
    ACTCGGCCCC AGAGCGACAC CCCAGAGATT CGCAAATACA AGAAGAGGTT TAACTCCGAG 
    ATCCTGTGCG CAGCCTTGTG GGGAGTGAAC TTGTTGGTGG GTACCGAGAG TGGCCTGATG 
    CTGCTGGACA GAAGTGGCCA AGGGAAGGTG TATCCTCTGA TCAACAGAAG GCGATTTCAG 
    CAGATGGACG TCCTGGAAGG CTTGAACGTC TTAGTGACGA TATCTGGCAA AAAGGATAAG 
    TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAACGA CCCGGAGGTT 
    GAGAAGAAGC AGGGCTGGAC GACAGTGGGG GACCTGGAAG GCTGTGTGCA TTATAAAGTT 
    GTAAAATATG AAAGGATCAA ATTTCTGGTC ATTGCTTTGA AGAACTCTGT GGAAGTCTAC 
    GCATGGGCAC CCAAGCCATA TCACAAATTC ATGGCCTTTA AGTCATTTGG TGAGCTGGTG 
    CACAAGCCGT TGCTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGCTGAA AGTGATCTAT 
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGACTCAG GATCGGTCTA TGACATTTAC 
    CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATCC AATGTAGCAT CAAACCCCAC 
    GCAATCATCA TCCTCCCCAA CACAGATGGC ATGGAGCTCC TGGTGTGCTA TGAAGATGAA 
    GGGGTTTACG TGAACACGTA CGGGAGGATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG 
    ATGCCAACGT CTGTAGCATA TATTCGATCC AATCAGACGA TGGGCTGGGG AGAAAAGGCC 
    ATAGAGATCC GATCTGTGGA AACTGGCCAC TTGGATGGCG TGTTTATGCA TAAAAGGGCT 
    CAAAGGCTGA AGTTCTTGTG TGAACGTAAT GACAAGGTGT TCTTTGCCTC CGTCCGGTCT 
    GGGGGCAGCA GCCAGGTTTA CTTCATGACT CTCGGCAGGA CCTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855093.1
    CDS121..4260
    Translation

    Target ORF information:

    RefSeq Version XM_024999325.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999325.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCACGGGTC CCTGTGAGAA CAACATCACG GTCCCCTGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGT AGTGGGCAGC AGAGTAACCA GGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCGAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCGG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CGGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGACCTG ACGGCTCTGG CCAAAGAGCT GCGGGCCGTG 
    GAGGATGTGC GGCCGCCGCA CAAGGTGACA GACTACTCCT CCTCCAGCGA GGAGTCGGGG 
    ACAACGGACG AGGAGGACGA CGACGTGGAG CAGGAGGGGG CCGAGGAGCC TACGTCTGGC 
    CCGGAGGACA CCAGAGCTGC ATCATCTCTG AACTTGAGCA ATGGTGAAAC AGAGTCCGTG 
    AAAACCATGG TTGTCCATGA CGACGTGGAG AGTGAACCAG CCCTGACCCC GTCCAAAGAG 
    GGCACGCTGA TCGTCCGCCA GAGTGCAGTT GACCAAAAGC GCGCCAGCCA TCATGAGAGC 
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
    TCCACTTCCT CCACCTCCTC TTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
    CAGACACCCC AGGACAAGCT CACTACTAAT GAGACTCAGT CCGCTAGTAA CACACTCCAG 
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
    CCATCTAGCG GGACAACAGT GACTTCTGTG GTGGGGTTTT CCTGTGATGG AATGAGACCT 
    GAGGCCATGA GGCAAGATCC TACGCGGAAG GGCTCAGTGG TCAATGTCAA TCCCACCAAT 
    ACTCGGCCCC AGAGCGACAC CCCAGAGATT CGCAAATACA AGAAGAGGTT TAACTCCGAG 
    ATCCTGTGCG CAGCCTTGTG GGGAGTGAAC TTGTTGGTGG GTACCGAGAG TGGCCTGATG 
    CTGCTGGACA GAAGTGGCCA AGGGAAGGTG TATCCTCTGA TCAACAGAAG GCGATTTCAG 
    CAGATGGACG TCCTGGAAGG CTTGAACGTC TTAGTGACGA TATCTGGCAA AAAGGATAAG 
    TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAACGA CCCGGAGGTT 
    GAGAAGAAGC AGGGCTGGAC GACAGTGGGG GACCTGGAAG GCTGTGTGCA TTATAAAGTT 
    GTAAAATATG AAAGGATCAA ATTTCTGGTC ATTGCTTTGA AGAACTCTGT GGAAGTCTAC 
    GCATGGGCAC CCAAGCCATA TCACAAATTC ATGGCCTTTA AGTCATTTGG TGAGCTGGTG 
    CACAAGCCGT TGCTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGCTGAA AGTGATCTAT 
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGACTCAG GATCGGTCTA TGACATTTAC 
    CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATCC AATGTAGCAT CAAACCCCAC 
    GCAATCATCA TCCTCCCCAA CACAGATGGC ATGGAGCTCC TGGTGTGCTA TGAAGATGAA 
    GGGGTTTACG TGAACACGTA CGGGAGGATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG 
    ATGCCAACGT CTGTAGCATA TATTCGATCC AATCAGACGA TGGGCTGGGG AGAAAAGGCC 
    ATAGAGATCC GATCTGTGGA AACTGGCCAC TTGGATGGCG TGTTTATGCA TAAAAGGGCT 
    CAAAGGCTGA AGTTCTTGTG TGAACGTAAT GACAAGGTGT TCTTTGCCTC CGTCCGGTCT 
    GGGGGCAGCA GCCAGGTTTA CTTCATGACT CTCGGCAGGA CCTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999326.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855094.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OBa220750
    Clone ID Related Accession (Same CDS sequence) XM_024999326.1
    Accession Version XM_024999326.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCACGGGTC CCTGTGAGAA CAACATCACG GTCCCCTGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGT AGTGGGCAGC AGAGTAACCA GGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCGAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCGG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CGGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCTCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGATGTGCG GCCGCCGCAC AAGGTGACAG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CAACGGACGA GGAGGACGAC GACGTGGAGC AGGAGGGGGC CGAGGAGCCT 
    ACGTCTGGCC CGGAGGACAC CAGAGCTGCA TCATCTCTGA ACTTGAGCAA TGGTGAAACA 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT 
    CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
    CATTCCTCCT CCACTTCCTC CACCTCCTCT TCCCCATCCT CCAGCCAGCC GACACCCACC 
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTACTAATG AGACTCAGTC CGCTAGTAAC 
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
    CAGATTTCTC CATCTAGCGG GACAACAGTG ACTTCTGTGG TGGGGTTTTC CTGTGATGGA 
    ATGAGACCTG AGGCCATGAG GCAAGATCCT ACGCGGAAGG GCTCAGTGGT CAATGTCAAT 
    CCCACCAATA CTCGGCCCCA GAGCGACACC CCAGAGATTC GCAAATACAA GAAGAGGTTT 
    AACTCCGAGA TCCTGTGCGC AGCCTTGTGG GGAGTGAACT TGTTGGTGGG TACCGAGAGT 
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTGT ATCCTCTGAT CAACAGAAGG 
    CGATTTCAGC AGATGGACGT CCTGGAAGGC TTGAACGTCT TAGTGACGAT ATCTGGCAAA 
    AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAACGAC 
    CCGGAGGTTG AGAAGAAGCA GGGCTGGACG ACAGTGGGGG ACCTGGAAGG CTGTGTGCAT 
    TATAAAGTTG TAAAATATGA AAGGATCAAA TTTCTGGTCA TTGCTTTGAA GAACTCTGTG 
    GAAGTCTACG CATGGGCACC CAAGCCATAT CACAAATTCA TGGCCTTTAA GTCATTTGGT 
    GAGCTGGTGC ACAAGCCGTT GCTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGCTGAAA 
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGACTCAGG ATCGGTCTAT 
    GACATTTACC TACCAACACA CATCCAATGT AGCATCAAAC CCCACGCAAT CATCATCCTC 
    CCCAACACAG ATGGCATGGA GCTCCTGGTG TGCTATGAAG ATGAAGGGGT TTACGTGAAC 
    ACGTACGGGA GGATCACCAA GGACGTGGTT CTGCAGTGGG GAGAGATGCC AACGTCTGTA 
    GCATATATTC GATCCAATCA GACGATGGGC TGGGGAGAAA AGGCCATAGA GATCCGATCT 
    GTGGAAACTG GCCACTTGGA TGGCGTGTTT ATGCATAAAA GGGCTCAAAG GCTGAAGTTC 
    TTGTGTGAAC GTAATGACAA GGTGTTCTTT GCCTCCGTCC GGTCTGGGGG CAGCAGCCAG 
    GTTTACTTCA TGACTCTCGG CAGGACCTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855094.1
    CDS121..4245
    Translation

    Target ORF information:

    RefSeq Version XM_024999326.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999326.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCACGGGTC CCTGTGAGAA CAACATCACG GTCCCCTGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGT AGTGGGCAGC AGAGTAACCA GGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCGAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCGG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CGGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCTCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGATGTGCG GCCGCCGCAC AAGGTGACAG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CAACGGACGA GGAGGACGAC GACGTGGAGC AGGAGGGGGC CGAGGAGCCT 
    ACGTCTGGCC CGGAGGACAC CAGAGCTGCA TCATCTCTGA ACTTGAGCAA TGGTGAAACA 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT 
    CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
    CATTCCTCCT CCACTTCCTC CACCTCCTCT TCCCCATCCT CCAGCCAGCC GACACCCACC 
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTACTAATG AGACTCAGTC CGCTAGTAAC 
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
    CAGATTTCTC CATCTAGCGG GACAACAGTG ACTTCTGTGG TGGGGTTTTC CTGTGATGGA 
    ATGAGACCTG AGGCCATGAG GCAAGATCCT ACGCGGAAGG GCTCAGTGGT CAATGTCAAT 
    CCCACCAATA CTCGGCCCCA GAGCGACACC CCAGAGATTC GCAAATACAA GAAGAGGTTT 
    AACTCCGAGA TCCTGTGCGC AGCCTTGTGG GGAGTGAACT TGTTGGTGGG TACCGAGAGT 
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTGT ATCCTCTGAT CAACAGAAGG 
    CGATTTCAGC AGATGGACGT CCTGGAAGGC TTGAACGTCT TAGTGACGAT ATCTGGCAAA 
    AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAACGAC 
    CCGGAGGTTG AGAAGAAGCA GGGCTGGACG ACAGTGGGGG ACCTGGAAGG CTGTGTGCAT 
    TATAAAGTTG TAAAATATGA AAGGATCAAA TTTCTGGTCA TTGCTTTGAA GAACTCTGTG 
    GAAGTCTACG CATGGGCACC CAAGCCATAT CACAAATTCA TGGCCTTTAA GTCATTTGGT 
    GAGCTGGTGC ACAAGCCGTT GCTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGCTGAAA 
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGACTCAGG ATCGGTCTAT 
    GACATTTACC TACCAACACA CATCCAATGT AGCATCAAAC CCCACGCAAT CATCATCCTC 
    CCCAACACAG ATGGCATGGA GCTCCTGGTG TGCTATGAAG ATGAAGGGGT TTACGTGAAC 
    ACGTACGGGA GGATCACCAA GGACGTGGTT CTGCAGTGGG GAGAGATGCC AACGTCTGTA 
    GCATATATTC GATCCAATCA GACGATGGGC TGGGGAGAAA AGGCCATAGA GATCCGATCT 
    GTGGAAACTG GCCACTTGGA TGGCGTGTTT ATGCATAAAA GGGCTCAAAG GCTGAAGTTC 
    TTGTGTGAAC GTAATGACAA GGTGTTCTTT GCCTCCGTCC GGTCTGGGGG CAGCAGCCAG 
    GTTTACTTCA TGACTCTCGG CAGGACCTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999327.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855095.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OBa220751
    Clone ID Related Accession (Same CDS sequence) XM_024999327.1
    Accession Version XM_024999327.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTGTTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCGAGTG GCAACTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTTT CCCCTGTCTC GAGATCCCAT 
    TCCTTCAGTG ACCCTTCTCC TCCCAAATTT GCACACCACC ATCTTCGCTC TCAGGACCCC 
    TGCCCACCTT CCCGCAGTGA GGTGCTCAGT CAGAGTTCTG ACTCTAAGGC GGAGGTGCCC 
    GACCCAGCCC AGAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC ACGGGTCCCT 
    GTGAGAACAA CATCACGGTC CCCTGTCCTG TCCCGCCGGG ATTCCCCACT GCAGGGTAGT 
    GGGCAGCAGA GTAACCAGGC TGGTCAGAGA AACTCCACCA GTAGCATCGA GCCGAGGCTG 
    CTCTGGGAGA GAGTGGAGAA GCTGGCGCCC AGGCCGGGCA GTGGCAGCTC CTCGGGGTCC 
    AGCAACTCGG GGTCCCAGCC CGGCTCCCAC CCAGGGTCTC AGAGTGGCTC CGGGGAGCGC 
    TTCAGAGTGA GATCATCATC CAAATCTGAA GGCTCGCCAT CTCAGCGCCT TGAAAACGCA 
    GCAAAAAAGC CTGAAGATAA AAAGGAAGTG TTTAGACCTC TCAAGCCTGC TGGTGAAGTG 
    GACCTGACGG CTCTGGCCAA AGAGCTGCGG GCCGTGGAGG ATGTGCGGCC GCCGCACAAG 
    GTGACAGACT ACTCCTCCTC CAGCGAGGAG TCGGGGACAA CGGACGAGGA GGACGACGAC 
    GTGGAGCAGG AGGGGGCCGA GGAGCCTACG TCTGGCCCGG AGGACACCAG AGCTGCATCA 
    TCTCTGAACT TGAGCAATGG TGAAACAGAG TCCGTGAAAA CCATGGTTGT CCATGACGAC 
    GTGGAGAGTG AACCAGCCCT GACCCCGTCC AAAGAGGGCA CGCTGATCGT CCGCCAGAGT 
    GCAGTTGACC AAAAGCGCGC CAGCCATCAT GAGAGCAATG GCTTTGCGGG TCGCATTCAC 
    CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCCTCTTCC 
    CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTCACT 
    ACTAATGAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGCGGGAC AACAGTGACT 
    TCTGTGGTGG GGTTTTCCTG TGATGGAATG AGACCTGAGG CCATGAGGCA AGATCCTACG 
    CGGAAGGGCT CAGTGGTCAA TGTCAATCCC ACCAATACTC GGCCCCAGAG CGACACCCCA 
    GAGATTCGCA AATACAAGAA GAGGTTTAAC TCCGAGATCC TGTGCGCAGC CTTGTGGGGA 
    GTGAACTTGT TGGTGGGTAC CGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG 
    AAGGTGTATC CTCTGATCAA CAGAAGGCGA TTTCAGCAGA TGGACGTCCT GGAAGGCTTG 
    AACGTCTTAG TGACGATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG 
    TTAAGAAATA AAATACTTCA CAACGACCCG GAGGTTGAGA AGAAGCAGGG CTGGACGACA 
    GTGGGGGACC TGGAAGGCTG TGTGCATTAT AAAGTTGTAA AATATGAAAG GATCAAATTT 
    CTGGTCATTG CTTTGAAGAA CTCTGTGGAA GTCTACGCAT GGGCACCCAA GCCATATCAC 
    AAATTCATGG CCTTTAAGTC ATTTGGTGAG CTGGTGCACA AGCCGTTGCT GGTGGATCTC 
    ACTGTTGAGG AAGGCCAGAG GCTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT 
    GTTGATGTGG ACTCAGGATC GGTCTATGAC ATTTACCTAC CAACACACGT AAGAAAGAAC 
    CCACACTCTA TGATCCAATG TAGCATCAAA CCCCACGCAA TCATCATCCT CCCCAACACA 
    GATGGCATGG AGCTCCTGGT GTGCTATGAA GATGAAGGGG TTTACGTGAA CACGTACGGG 
    AGGATCACCA AGGACGTGGT TCTGCAGTGG GGAGAGATGC CAACGTCTGT AGCATATATT 
    CGATCCAATC AGACGATGGG CTGGGGAGAA AAGGCCATAG AGATCCGATC TGTGGAAACT 
    GGCCACTTGG ATGGCGTGTT TATGCATAAA AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA 
    CGTAATGACA AGGTGTTCTT TGCCTCCGTC CGGTCTGGGG GCAGCAGCCA GGTTTACTTC 
    ATGACTCTCG GCAGGACCTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855095.1
    CDS123..4178
    Translation

    Target ORF information:

    RefSeq Version XM_024999327.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999327.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTGTTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCGAGTG GCAACTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTTT CCCCTGTCTC GAGATCCCAT 
    TCCTTCAGTG ACCCTTCTCC TCCCAAATTT GCACACCACC ATCTTCGCTC TCAGGACCCC 
    TGCCCACCTT CCCGCAGTGA GGTGCTCAGT CAGAGTTCTG ACTCTAAGGC GGAGGTGCCC 
    GACCCAGCCC AGAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC ACGGGTCCCT 
    GTGAGAACAA CATCACGGTC CCCTGTCCTG TCCCGCCGGG ATTCCCCACT GCAGGGTAGT 
    GGGCAGCAGA GTAACCAGGC TGGTCAGAGA AACTCCACCA GTAGCATCGA GCCGAGGCTG 
    CTCTGGGAGA GAGTGGAGAA GCTGGCGCCC AGGCCGGGCA GTGGCAGCTC CTCGGGGTCC 
    AGCAACTCGG GGTCCCAGCC CGGCTCCCAC CCAGGGTCTC AGAGTGGCTC CGGGGAGCGC 
    TTCAGAGTGA GATCATCATC CAAATCTGAA GGCTCGCCAT CTCAGCGCCT TGAAAACGCA 
    GCAAAAAAGC CTGAAGATAA AAAGGAAGTG TTTAGACCTC TCAAGCCTGC TGGTGAAGTG 
    GACCTGACGG CTCTGGCCAA AGAGCTGCGG GCCGTGGAGG ATGTGCGGCC GCCGCACAAG 
    GTGACAGACT ACTCCTCCTC CAGCGAGGAG TCGGGGACAA CGGACGAGGA GGACGACGAC 
    GTGGAGCAGG AGGGGGCCGA GGAGCCTACG TCTGGCCCGG AGGACACCAG AGCTGCATCA 
    TCTCTGAACT TGAGCAATGG TGAAACAGAG TCCGTGAAAA CCATGGTTGT CCATGACGAC 
    GTGGAGAGTG AACCAGCCCT GACCCCGTCC AAAGAGGGCA CGCTGATCGT CCGCCAGAGT 
    GCAGTTGACC AAAAGCGCGC CAGCCATCAT GAGAGCAATG GCTTTGCGGG TCGCATTCAC 
    CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCCTCTTCC 
    CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTCACT 
    ACTAATGAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGCGGGAC AACAGTGACT 
    TCTGTGGTGG GGTTTTCCTG TGATGGAATG AGACCTGAGG CCATGAGGCA AGATCCTACG 
    CGGAAGGGCT CAGTGGTCAA TGTCAATCCC ACCAATACTC GGCCCCAGAG CGACACCCCA 
    GAGATTCGCA AATACAAGAA GAGGTTTAAC TCCGAGATCC TGTGCGCAGC CTTGTGGGGA 
    GTGAACTTGT TGGTGGGTAC CGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG 
    AAGGTGTATC CTCTGATCAA CAGAAGGCGA TTTCAGCAGA TGGACGTCCT GGAAGGCTTG 
    AACGTCTTAG TGACGATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG 
    TTAAGAAATA AAATACTTCA CAACGACCCG GAGGTTGAGA AGAAGCAGGG CTGGACGACA 
    GTGGGGGACC TGGAAGGCTG TGTGCATTAT AAAGTTGTAA AATATGAAAG GATCAAATTT 
    CTGGTCATTG CTTTGAAGAA CTCTGTGGAA GTCTACGCAT GGGCACCCAA GCCATATCAC 
    AAATTCATGG CCTTTAAGTC ATTTGGTGAG CTGGTGCACA AGCCGTTGCT GGTGGATCTC 
    ACTGTTGAGG AAGGCCAGAG GCTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT 
    GTTGATGTGG ACTCAGGATC GGTCTATGAC ATTTACCTAC CAACACACGT AAGAAAGAAC 
    CCACACTCTA TGATCCAATG TAGCATCAAA CCCCACGCAA TCATCATCCT CCCCAACACA 
    GATGGCATGG AGCTCCTGGT GTGCTATGAA GATGAAGGGG TTTACGTGAA CACGTACGGG 
    AGGATCACCA AGGACGTGGT TCTGCAGTGG GGAGAGATGC CAACGTCTGT AGCATATATT 
    CGATCCAATC AGACGATGGG CTGGGGAGAA AAGGCCATAG AGATCCGATC TGTGGAAACT 
    GGCCACTTGG ATGGCGTGTT TATGCATAAA AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA 
    CGTAATGACA AGGTGTTCTT TGCCTCCGTC CGGTCTGGGG GCAGCAGCCA GGTTTACTTC 
    ATGACTCTCG GCAGGACCTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999328.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855096.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OBa220752
    Clone ID Related Accession (Same CDS sequence) XM_024999328.1
    Accession Version XM_024999328.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTACAGTGG 
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGAGATCCCA TTCCTTCAGT 
    GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGCT CTCAGGACCC CTGCCCACCT 
    TCCCGCAGTG AGGTGCTCAG TCAGAGTTCT GACTCTAAGG CGGAGGTGCC CGACCCAGCC 
    CAGAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CACGGGTCCC TGTGAGAACA 
    ACATCACGGT CCCCTGTCCT GTCCCGCCGG GATTCCCCAC TGCAGGGTAG TGGGCAGCAG 
    AGTAACCAGG CTGGTCAGAG AAACTCCACC AGTAGCATCG AGCCGAGGCT GCTCTGGGAG 
    AGAGTGGAGA AGCTGGCGCC CAGGCCGGGC AGTGGCAGCT CCTCGGGGTC CAGCAACTCG 
    GGGTCCCAGC CCGGCTCCCA CCCAGGGTCT CAGAGTGGCT CCGGGGAGCG CTTCAGAGTG 
    AGATCATCAT CCAAATCTGA AGGCTCGCCA TCTCAGCGCC TTGAAAACGC AGCAAAAAAG 
    CCTGAAGATA AAAAGGAAGT GTTTAGACCT CTCAAGCCTG CTGGTGAAGT GGACCTGACG 
    GCTCTGGCCA AAGAGCTGCG GGCCGTGGAG GATGTGCGGC CGCCGCACAA GGTGACAGAC 
    TACTCCTCCT CCAGCGAGGA GTCGGGGACA ACGGACGAGG AGGACGACGA CGTGGAGCAG 
    GAGGGGGCCG AGGAGCCTAC GTCTGGCCCG GAGGACACCA GAGCTGCATC ATCTCTGAAC 
    TTGAGCAATG GTGAAACAGA GTCCGTGAAA ACCATGGTTG TCCATGACGA CGTGGAGAGT 
    GAACCAGCCC TGACCCCGTC CAAAGAGGGC ACGCTGATCG TCCGCCAGAG TGCAGTTGAC 
    CAAAAGCGCG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA 
    GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCCTCTTC CCCATCCTCC 
    AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TACTAATGAG 
    ACTCAGTCCG CTAGTAACAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
    ATAGACCCCA GATTACTACA GATTTCTCCA TCTAGCGGGA CAACAGTGAC TTCTGTGGTG 
    GGGTTTTCCT GTGATGGAAT GAGACCTGAG GCCATGAGGC AAGATCCTAC GCGGAAGGGC 
    TCAGTGGTCA ATGTCAATCC CACCAATACT CGGCCCCAGA GCGACACCCC AGAGATTCGC 
    AAATACAAGA AGAGGTTTAA CTCCGAGATC CTGTGCGCAG CCTTGTGGGG AGTGAACTTG 
    TTGGTGGGTA CCGAGAGTGG CCTGATGCTG CTGGACAGAA GTGGCCAAGG GAAGGTGTAT 
    CCTCTGATCA ACAGAAGGCG ATTTCAGCAG ATGGACGTCC TGGAAGGCTT GAACGTCTTA 
    GTGACGATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTGTCCTG GTTAAGAAAT 
    AAAATACTTC ACAACGACCC GGAGGTTGAG AAGAAGCAGG GCTGGACGAC AGTGGGGGAC 
    CTGGAAGGCT GTGTGCATTA TAAAGTTGTA AAATATGAAA GGATCAAATT TCTGGTCATT 
    GCTTTGAAGA ACTCTGTGGA AGTCTACGCA TGGGCACCCA AGCCATATCA CAAATTCATG 
    GCCTTTAAGT CATTTGGTGA GCTGGTGCAC AAGCCGTTGC TGGTGGATCT CACTGTTGAG 
    GAAGGCCAGA GGCTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG 
    GACTCAGGAT CGGTCTATGA CATTTACCTA CCAACACACG TAAGAAAGAA CCCACACTCT 
    ATGATCCAAT GTAGCATCAA ACCCCACGCA ATCATCATCC TCCCCAACAC AGATGGCATG 
    GAGCTCCTGG TGTGCTATGA AGATGAAGGG GTTTACGTGA ACACGTACGG GAGGATCACC 
    AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCAACGTCTG TAGCATATAT TCGATCCAAT 
    CAGACGATGG GCTGGGGAGA AAAGGCCATA GAGATCCGAT CTGTGGAAAC TGGCCACTTG 
    GATGGCGTGT TTATGCATAA AAGGGCTCAA AGGCTGAAGT TCTTGTGTGA ACGTAATGAC 
    AAGGTGTTCT TTGCCTCCGT CCGGTCTGGG GGCAGCAGCC AGGTTTACTT CATGACTCTC 
    GGCAGGACCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855096.1
    CDS121..4107
    Translation

    Target ORF information:

    RefSeq Version XM_024999328.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999328.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTACAGTGG 
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGAGATCCCA TTCCTTCAGT 
    GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGCT CTCAGGACCC CTGCCCACCT 
    TCCCGCAGTG AGGTGCTCAG TCAGAGTTCT GACTCTAAGG CGGAGGTGCC CGACCCAGCC 
    CAGAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CACGGGTCCC TGTGAGAACA 
    ACATCACGGT CCCCTGTCCT GTCCCGCCGG GATTCCCCAC TGCAGGGTAG TGGGCAGCAG 
    AGTAACCAGG CTGGTCAGAG AAACTCCACC AGTAGCATCG AGCCGAGGCT GCTCTGGGAG 
    AGAGTGGAGA AGCTGGCGCC CAGGCCGGGC AGTGGCAGCT CCTCGGGGTC CAGCAACTCG 
    GGGTCCCAGC CCGGCTCCCA CCCAGGGTCT CAGAGTGGCT CCGGGGAGCG CTTCAGAGTG 
    AGATCATCAT CCAAATCTGA AGGCTCGCCA TCTCAGCGCC TTGAAAACGC AGCAAAAAAG 
    CCTGAAGATA AAAAGGAAGT GTTTAGACCT CTCAAGCCTG CTGGTGAAGT GGACCTGACG 
    GCTCTGGCCA AAGAGCTGCG GGCCGTGGAG GATGTGCGGC CGCCGCACAA GGTGACAGAC 
    TACTCCTCCT CCAGCGAGGA GTCGGGGACA ACGGACGAGG AGGACGACGA CGTGGAGCAG 
    GAGGGGGCCG AGGAGCCTAC GTCTGGCCCG GAGGACACCA GAGCTGCATC ATCTCTGAAC 
    TTGAGCAATG GTGAAACAGA GTCCGTGAAA ACCATGGTTG TCCATGACGA CGTGGAGAGT 
    GAACCAGCCC TGACCCCGTC CAAAGAGGGC ACGCTGATCG TCCGCCAGAG TGCAGTTGAC 
    CAAAAGCGCG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA 
    GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCCTCTTC CCCATCCTCC 
    AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TACTAATGAG 
    ACTCAGTCCG CTAGTAACAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
    ATAGACCCCA GATTACTACA GATTTCTCCA TCTAGCGGGA CAACAGTGAC TTCTGTGGTG 
    GGGTTTTCCT GTGATGGAAT GAGACCTGAG GCCATGAGGC AAGATCCTAC GCGGAAGGGC 
    TCAGTGGTCA ATGTCAATCC CACCAATACT CGGCCCCAGA GCGACACCCC AGAGATTCGC 
    AAATACAAGA AGAGGTTTAA CTCCGAGATC CTGTGCGCAG CCTTGTGGGG AGTGAACTTG 
    TTGGTGGGTA CCGAGAGTGG CCTGATGCTG CTGGACAGAA GTGGCCAAGG GAAGGTGTAT 
    CCTCTGATCA ACAGAAGGCG ATTTCAGCAG ATGGACGTCC TGGAAGGCTT GAACGTCTTA 
    GTGACGATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTGTCCTG GTTAAGAAAT 
    AAAATACTTC ACAACGACCC GGAGGTTGAG AAGAAGCAGG GCTGGACGAC AGTGGGGGAC 
    CTGGAAGGCT GTGTGCATTA TAAAGTTGTA AAATATGAAA GGATCAAATT TCTGGTCATT 
    GCTTTGAAGA ACTCTGTGGA AGTCTACGCA TGGGCACCCA AGCCATATCA CAAATTCATG 
    GCCTTTAAGT CATTTGGTGA GCTGGTGCAC AAGCCGTTGC TGGTGGATCT CACTGTTGAG 
    GAAGGCCAGA GGCTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG 
    GACTCAGGAT CGGTCTATGA CATTTACCTA CCAACACACG TAAGAAAGAA CCCACACTCT 
    ATGATCCAAT GTAGCATCAA ACCCCACGCA ATCATCATCC TCCCCAACAC AGATGGCATG 
    GAGCTCCTGG TGTGCTATGA AGATGAAGGG GTTTACGTGA ACACGTACGG GAGGATCACC 
    AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCAACGTCTG TAGCATATAT TCGATCCAAT 
    CAGACGATGG GCTGGGGAGA AAAGGCCATA GAGATCCGAT CTGTGGAAAC TGGCCACTTG 
    GATGGCGTGT TTATGCATAA AAGGGCTCAA AGGCTGAAGT TCTTGTGTGA ACGTAATGAC 
    AAGGTGTTCT TTGCCTCCGT CCGGTCTGGG GGCAGCAGCC AGGTTTACTT CATGACTCTC 
    GGCAGGACCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999329.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855097.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID OBa220753
    Clone ID Related Accession (Same CDS sequence) XM_024999329.1
    Accession Version XM_024999329.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTACAGTGG 
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGAGATCCCA TTCCTTCAGT 
    GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGCT CTCAGGACCC CTGCCCACCT 
    TCCCGCAGTG AGGTGCTCAG TCAGAGTTCT GACTCTAAGG CGGAGGTGCC CGACCCAGCC 
    CAGAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CACGGGTCCC TGTGAGAACA 
    ACATCACGGT CCCCTGTCCT GTCCCGCCGG GATTCCCCAC TGCAGGGTAG TGGGCAGCAG 
    AGTAACCAGG CTGGTCAGAG AAACTCCACC AGCATCGAGC CGAGGCTGCT CTGGGAGAGA 
    GTGGAGAAGC TGGCGCCCAG GCCGGGCAGT GGCAGCTCCT CGGGGTCCAG CAACTCGGGG 
    TCCCAGCCCG GCTCCCACCC AGGGTCTCAG AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA 
    TCATCATCCA AATCTGAAGG CTCGCCATCT CAGCGCCTTG AAAACGCAGC AAAAAAGCCT 
    GAAGATAAAA AGGAAGTGTT TAGACCTCTC AAGCCTGCTG GTGAAGTGGA CCTGACGGCT 
    CTGGCCAAAG AGCTGCGGGC CGTGGAGGAT GTGCGGCCGC CGCACAAGGT GACAGACTAC 
    TCCTCCTCCA GCGAGGAGTC GGGGACAACG GACGAGGAGG ACGACGACGT GGAGCAGGAG 
    GGGGCCGAGG AGCCTACGTC TGGCCCGGAG GACACCAGAG CTGCATCATC TCTGAACTTG 
    AGCAATGGTG AAACAGAGTC CGTGAAAACC ATGGTTGTCC ATGACGACGT GGAGAGTGAA 
    CCAGCCCTGA CCCCGTCCAA AGAGGGCACG CTGATCGTCC GCCAGAGTGC AGTTGACCAA 
    AAGCGCGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT 
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCTTCCCC ATCCTCCAGC 
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTAC TAATGAGACT 
    CAGTCCGCTA GTAACACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGGACAA CAGTGACTTC TGTGGTGGGG 
    TTTTCCTGTG ATGGAATGAG ACCTGAGGCC ATGAGGCAAG ATCCTACGCG GAAGGGCTCA 
    GTGGTCAATG TCAATCCCAC CAATACTCGG CCCCAGAGCG ACACCCCAGA GATTCGCAAA 
    TACAAGAAGA GGTTTAACTC CGAGATCCTG TGCGCAGCCT TGTGGGGAGT GAACTTGTTG 
    GTGGGTACCG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTGTATCCT 
    CTGATCAACA GAAGGCGATT TCAGCAGATG GACGTCCTGG AAGGCTTGAA CGTCTTAGTG 
    ACGATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
    ATACTTCACA ACGACCCGGA GGTTGAGAAG AAGCAGGGCT GGACGACAGT GGGGGACCTG 
    GAAGGCTGTG TGCATTATAA AGTTGTAAAA TATGAAAGGA TCAAATTTCT GGTCATTGCT 
    TTGAAGAACT CTGTGGAAGT CTACGCATGG GCACCCAAGC CATATCACAA ATTCATGGCC 
    TTTAAGTCAT TTGGTGAGCT GGTGCACAAG CCGTTGCTGG TGGATCTCAC TGTTGAGGAA 
    GGCCAGAGGC TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAC 
    TCAGGATCGG TCTATGACAT TTACCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG 
    ATCCAATGTA GCATCAAACC CCACGCAATC ATCATCCTCC CCAACACAGA TGGCATGGAG 
    CTCCTGGTGT GCTATGAAGA TGAAGGGGTT TACGTGAACA CGTACGGGAG GATCACCAAG 
    GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACGTCTGTAG CATATATTCG ATCCAATCAG 
    ACGATGGGCT GGGGAGAAAA GGCCATAGAG ATCCGATCTG TGGAAACTGG CCACTTGGAT 
    GGCGTGTTTA TGCATAAAAG GGCTCAAAGG CTGAAGTTCT TGTGTGAACG TAATGACAAG 
    GTGTTCTTTG CCTCCGTCCG GTCTGGGGGC AGCAGCCAGG TTTACTTCAT GACTCTCGGC 
    AGGACCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855097.1
    CDS121..4104
    Translation

    Target ORF information:

    RefSeq Version XM_024999329.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999329.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTACAGTGG 
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGAGATCCCA TTCCTTCAGT 
    GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGCT CTCAGGACCC CTGCCCACCT 
    TCCCGCAGTG AGGTGCTCAG TCAGAGTTCT GACTCTAAGG CGGAGGTGCC CGACCCAGCC 
    CAGAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CACGGGTCCC TGTGAGAACA 
    ACATCACGGT CCCCTGTCCT GTCCCGCCGG GATTCCCCAC TGCAGGGTAG TGGGCAGCAG 
    AGTAACCAGG CTGGTCAGAG AAACTCCACC AGCATCGAGC CGAGGCTGCT CTGGGAGAGA 
    GTGGAGAAGC TGGCGCCCAG GCCGGGCAGT GGCAGCTCCT CGGGGTCCAG CAACTCGGGG 
    TCCCAGCCCG GCTCCCACCC AGGGTCTCAG AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA 
    TCATCATCCA AATCTGAAGG CTCGCCATCT CAGCGCCTTG AAAACGCAGC AAAAAAGCCT 
    GAAGATAAAA AGGAAGTGTT TAGACCTCTC AAGCCTGCTG GTGAAGTGGA CCTGACGGCT 
    CTGGCCAAAG AGCTGCGGGC CGTGGAGGAT GTGCGGCCGC CGCACAAGGT GACAGACTAC 
    TCCTCCTCCA GCGAGGAGTC GGGGACAACG GACGAGGAGG ACGACGACGT GGAGCAGGAG 
    GGGGCCGAGG AGCCTACGTC TGGCCCGGAG GACACCAGAG CTGCATCATC TCTGAACTTG 
    AGCAATGGTG AAACAGAGTC CGTGAAAACC ATGGTTGTCC ATGACGACGT GGAGAGTGAA 
    CCAGCCCTGA CCCCGTCCAA AGAGGGCACG CTGATCGTCC GCCAGAGTGC AGTTGACCAA 
    AAGCGCGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT 
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCTTCCCC ATCCTCCAGC 
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTAC TAATGAGACT 
    CAGTCCGCTA GTAACACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGGACAA CAGTGACTTC TGTGGTGGGG 
    TTTTCCTGTG ATGGAATGAG ACCTGAGGCC ATGAGGCAAG ATCCTACGCG GAAGGGCTCA 
    GTGGTCAATG TCAATCCCAC CAATACTCGG CCCCAGAGCG ACACCCCAGA GATTCGCAAA 
    TACAAGAAGA GGTTTAACTC CGAGATCCTG TGCGCAGCCT TGTGGGGAGT GAACTTGTTG 
    GTGGGTACCG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTGTATCCT 
    CTGATCAACA GAAGGCGATT TCAGCAGATG GACGTCCTGG AAGGCTTGAA CGTCTTAGTG 
    ACGATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
    ATACTTCACA ACGACCCGGA GGTTGAGAAG AAGCAGGGCT GGACGACAGT GGGGGACCTG 
    GAAGGCTGTG TGCATTATAA AGTTGTAAAA TATGAAAGGA TCAAATTTCT GGTCATTGCT 
    TTGAAGAACT CTGTGGAAGT CTACGCATGG GCACCCAAGC CATATCACAA ATTCATGGCC 
    TTTAAGTCAT TTGGTGAGCT GGTGCACAAG CCGTTGCTGG TGGATCTCAC TGTTGAGGAA 
    GGCCAGAGGC TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAC 
    TCAGGATCGG TCTATGACAT TTACCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG 
    ATCCAATGTA GCATCAAACC CCACGCAATC ATCATCCTCC CCAACACAGA TGGCATGGAG 
    CTCCTGGTGT GCTATGAAGA TGAAGGGGTT TACGTGAACA CGTACGGGAG GATCACCAAG 
    GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACGTCTGTAG CATATATTCG ATCCAATCAG 
    ACGATGGGCT GGGGAGAAAA GGCCATAGAG ATCCGATCTG TGGAAACTGG CCACTTGGAT 
    GGCGTGTTTA TGCATAAAAG GGCTCAAAGG CTGAAGTTCT TGTGTGAACG TAATGACAAG 
    GTGTTCTTTG CCTCCGTCCG GTCTGGGGGC AGCAGCCAGG TTTACTTCAT GACTCTCGGC 
    AGGACCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999330.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855098.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID OBa220754
    Clone ID Related Accession (Same CDS sequence) XM_024999330.1 , XM_024999331.1
    Accession Version XM_024999330.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTTA CCGAGGATGA AGAGGAGGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACGTAT TACGGTGCTT TCATTAAAAA GAGCCCCCCA 
    GGACACGATG ACCAGCTTTG GCTCGTTATG GAGTTCTGTG GGGCCGGATC CATCACGGAC 
    CTGGTGAAGA ACACCAAGGG CAACACGCTA AAGGAAGACT GGATAGCCTA CATCTCCAGG 
    GAGATCCTTC GGGGCCTGGC CCATCTCCAC ATCCATCACG TGATCCACCG CGACATCAAG 
    GGCCAGAATG TGCTGCTGAC CGAGAACGCA GAGGTGAAGC TGGTGGACTT CGGGGTGAGT 
    GCCCAGCTGG ACAGGACCGT GGGGAGGAGG AACACGTTCA TAGGCACCCC GTACTGGATG 
    GCCCCTGAGG TCATTGCCTG TGACGAGAAC CCCGACGCCA CCTACGATTA CAGGAGTGAC 
    CTCTGGTCCT GTGGCATCAC TGCCATTGAG ATGGCTGAAG GCGCTCCCCC TCTCTGCGAC 
    ATGCATCCCA TGAGAGCCCT GTTTCTCATC CCCCGGAATC CTCCTCCCCG GCTGAAGTCC 
    AAAAAATGGT CCAAGAAGTT TTTTAGTTTT ATAGAAGGAT GCCTGGTGAA GAATTACATG 
    CAGCGGCCCT CCACCGAGCA GCTTCTGAAG CATCCTTTCA TTAGGGATCA GCCGAACGAA 
    AGGCAGGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAAA CCGAGTATGA GTACAGTGGG AGTGAGGAAG AGGAGGAGGA GGCGCCTGAG 
    CAGGAGGGGG AGCCCAGCTC CATCGTCAAC GTGCCGGGGG AGTCGACGCT GCGCCGGGAC 
    TTCCTGCGGC TCCAGCAGGA GAACAAGGAG CGCTCTGAGG CCCTGAGGAG GCAGCAGCTC 
    CTGCAGGAGC AGCAGCTCCG CGAGCAGGAG GAGTATAAAC GGCAGCTGCT GGCGGAGAGG 
    CAGAAGCGGA TCGAGCAGCA GAAGGAGCAG AGGCGGCGGC TGGAAGAGCA ACAGAGGAGA 
    GAGCGGGAAG CCAGAAGGCA GCAGGAACGT GAGCAGCGGA GGCGGGAACA GGAGGAGAAG 
    AGGCGGCTGG AGGAGCTGGA GAGACGACGG AAGGAGGAGG AGGAGCGGAG GCGGGCGGAG 
    GAGGAGAAGA GGAGAGTGGA GAGGGAGCAG GAGTATATCC GACGGCAGCT GGAAGAGGAA 
    CAGCGTCACC TGGAACTGCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GCTACTGGAG 
    TGTCGCTGGC GGGAGCTGGA GGAGCACCGG CAGGCAGAGC GGCTCCAGAG GCAGCTGCAG 
    CAAGAACAAG CCTATCTCCT GTCTCTACAG CACGACCCCC GGAGGCCGCC CCCGCCGCCA 
    GACAGGAGCA AGGCTGGCCT GCACGAGCCC AAGGCCCCGC ACGAGCCCGC TGACCGGGCC 
    CGTGAGGTGG AAGATAGATT TAGGAAAACT AACCACAGCT CCCCCGAAGC CCAGTCTAAG 
    CAGACGGGCC GAGTGTTGGA ACCTCCAGTG CCTTCCAGAT CAGAGTCTTT TTCGAGTGGC 
    AACTCCGAGT CTGTGCACCC TGCCCTGCAG AGACCCGCCG AGCCACAGGT ACAGTGGTCC 
    CACCTGGCAT CTCTCAAGAA CAATGTTTCC CCTGTCTCGA GATCCCATTC CTTCAGTGAC 
    CCTTCTCCTC CCAAATTTGC ACACCACCAT CTTCGCTCTC AGGACCCCTG CCCACCTTCC 
    CGCAGTGAGG TGCTCAGTCA GAGTTCTGAC TCTAAGGCGG AGGTGCCCGA CCCAGCCCAG 
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAC GGGTCCCTGT GAGAACAACA 
    TCACGGTCCC CTGTCCTGTC CCGCCGGGAT TCCCCACTGC AGGGTAGTGG GCAGCAGAGT 
    AACCAGGCTG GTCAGAGAAA CTCCACCAGT AGCATCGAGC CGAGGCTGCT CTGGGAGAGA 
    GTGGAGAAGC TGGCGCCCAG GCCGGGCAGT GGCAGCTCCT CGGGGTCCAG CAACTCGGGG 
    TCCCAGCCCG GCTCCCACCC AGGGTCTCAG AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA 
    TCATCATCCA AATCTGAAGG CTCGCCATCT CAGCGCCTTG AAAACGCAGC AAAAAAGCCT 
    GAAGATAAAA AGGAAGTGTT TAGACCTCTC AAGCCTGCTG GTGAAGTGGA CCTGACGGCT 
    CTGGCCAAAG AGCTGCGGGC CGTGGAGGAT GTGCGGCCGC CGCACAAGGT GACAGACTAC 
    TCCTCCTCCA GCGAGGAGTC GGGGACAACG GACGAGGAGG ACGACGACGT GGAGCAGGAG 
    GGGGCCGAGG AGCCTACGTC TGGCCCGGAG GACACCAGAG CTGCATCATC TCTGAACTTG 
    AGCAATGGTG AAACAGAGTC CGTGAAAACC ATGGTTGTCC ATGACGACGT GGAGAGTGAA 
    CCAGCCCTGA CCCCGTCCAA AGAGGGCACG CTGATCGTCC GCCAGAGTGC AGTTGACCAA 
    AAGCGCGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT 
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCTTCCCC ATCCTCCAGC 
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTAC TAATGAGACT 
    CAGTCCGCTA GTAACACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGGACAA CAGTGACTTC TGTGGTGGGG 
    TTTTCCTGTG ATGGAATGAG ACCTGAGGCC ATGAGGCAAG ATCCTACGCG GAAGGGCTCA 
    GTGGTCAATG TCAATCCCAC CAATACTCGG CCCCAGAGCG ACACCCCAGA GATTCGCAAA 
    TACAAGAAGA GGTTTAACTC CGAGATCCTG TGCGCAGCCT TGTGGGGAGT GAACTTGTTG 
    GTGGGTACCG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTGTATCCT 
    CTGATCAACA GAAGGCGATT TCAGCAGATG GACGTCCTGG AAGGCTTGAA CGTCTTAGTG 
    ACGATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
    ATACTTCACA ACGACCCGGA GGTTGAGAAG AAGCAGGGCT GGACGACAGT GGGGGACCTG 
    GAAGGCTGTG TGCATTATAA AGTTGTAAAA TATGAAAGGA TCAAATTTCT GGTCATTGCT 
    TTGAAGAACT CTGTGGAAGT CTACGCATGG GCACCCAAGC CATATCACAA ATTCATGGCC 
    TTTAAGTCAT TTGGTGAGCT GGTGCACAAG CCGTTGCTGG TGGATCTCAC TGTTGAGGAA 
    GGCCAGAGGC TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAC 
    TCAGGATCGG TCTATGACAT TTACCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG 
    ATCCAATGTA GCATCAAACC CCACGCAATC ATCATCCTCC CCAACACAGA TGGCATGGAG 
    CTCCTGGTGT GCTATGAAGA TGAAGGGGTT TACGTGAACA CGTACGGGAG GATCACCAAG 
    GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACGTCTGTAG CATATATTCG ATCCAATCAG 
    ACGATGGGCT GGGGAGAAAA GGCCATAGAG ATCCGATCTG TGGAAACTGG CCACTTGGAT 
    GGCGTGTTTA TGCATAAAAG GGCTCAAAGG CTGAAGTTCT TGTGTGAACG TAATGACAAG 
    GTGTTCTTTG CCTCCGTCCG GTCTGGGGGC AGCAGCCAGG TTTACTTCAT GACTCTCGGC 
    AGGACCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855098.1
    CDS107..4090
    Translation

    Target ORF information:

    RefSeq Version XM_024999330.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999330.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTTA CCGAGGATGA AGAGGAGGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACGTAT TACGGTGCTT TCATTAAAAA GAGCCCCCCA 
    GGACACGATG ACCAGCTTTG GCTCGTTATG GAGTTCTGTG GGGCCGGATC CATCACGGAC 
    CTGGTGAAGA ACACCAAGGG CAACACGCTA AAGGAAGACT GGATAGCCTA CATCTCCAGG 
    GAGATCCTTC GGGGCCTGGC CCATCTCCAC ATCCATCACG TGATCCACCG CGACATCAAG 
    GGCCAGAATG TGCTGCTGAC CGAGAACGCA GAGGTGAAGC TGGTGGACTT CGGGGTGAGT 
    GCCCAGCTGG ACAGGACCGT GGGGAGGAGG AACACGTTCA TAGGCACCCC GTACTGGATG 
    GCCCCTGAGG TCATTGCCTG TGACGAGAAC CCCGACGCCA CCTACGATTA CAGGAGTGAC 
    CTCTGGTCCT GTGGCATCAC TGCCATTGAG ATGGCTGAAG GCGCTCCCCC TCTCTGCGAC 
    ATGCATCCCA TGAGAGCCCT GTTTCTCATC CCCCGGAATC CTCCTCCCCG GCTGAAGTCC 
    AAAAAATGGT CCAAGAAGTT TTTTAGTTTT ATAGAAGGAT GCCTGGTGAA GAATTACATG 
    CAGCGGCCCT CCACCGAGCA GCTTCTGAAG CATCCTTTCA TTAGGGATCA GCCGAACGAA 
    AGGCAGGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAAA CCGAGTATGA GTACAGTGGG AGTGAGGAAG AGGAGGAGGA GGCGCCTGAG 
    CAGGAGGGGG AGCCCAGCTC CATCGTCAAC GTGCCGGGGG AGTCGACGCT GCGCCGGGAC 
    TTCCTGCGGC TCCAGCAGGA GAACAAGGAG CGCTCTGAGG CCCTGAGGAG GCAGCAGCTC 
    CTGCAGGAGC AGCAGCTCCG CGAGCAGGAG GAGTATAAAC GGCAGCTGCT GGCGGAGAGG 
    CAGAAGCGGA TCGAGCAGCA GAAGGAGCAG AGGCGGCGGC TGGAAGAGCA ACAGAGGAGA 
    GAGCGGGAAG CCAGAAGGCA GCAGGAACGT GAGCAGCGGA GGCGGGAACA GGAGGAGAAG 
    AGGCGGCTGG AGGAGCTGGA GAGACGACGG AAGGAGGAGG AGGAGCGGAG GCGGGCGGAG 
    GAGGAGAAGA GGAGAGTGGA GAGGGAGCAG GAGTATATCC GACGGCAGCT GGAAGAGGAA 
    CAGCGTCACC TGGAACTGCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GCTACTGGAG 
    TGTCGCTGGC GGGAGCTGGA GGAGCACCGG CAGGCAGAGC GGCTCCAGAG GCAGCTGCAG 
    CAAGAACAAG CCTATCTCCT GTCTCTACAG CACGACCCCC GGAGGCCGCC CCCGCCGCCA 
    GACAGGAGCA AGGCTGGCCT GCACGAGCCC AAGGCCCCGC ACGAGCCCGC TGACCGGGCC 
    CGTGAGGTGG AAGATAGATT TAGGAAAACT AACCACAGCT CCCCCGAAGC CCAGTCTAAG 
    CAGACGGGCC GAGTGTTGGA ACCTCCAGTG CCTTCCAGAT CAGAGTCTTT TTCGAGTGGC 
    AACTCCGAGT CTGTGCACCC TGCCCTGCAG AGACCCGCCG AGCCACAGGT ACAGTGGTCC 
    CACCTGGCAT CTCTCAAGAA CAATGTTTCC CCTGTCTCGA GATCCCATTC CTTCAGTGAC 
    CCTTCTCCTC CCAAATTTGC ACACCACCAT CTTCGCTCTC AGGACCCCTG CCCACCTTCC 
    CGCAGTGAGG TGCTCAGTCA GAGTTCTGAC TCTAAGGCGG AGGTGCCCGA CCCAGCCCAG 
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAC GGGTCCCTGT GAGAACAACA 
    TCACGGTCCC CTGTCCTGTC CCGCCGGGAT TCCCCACTGC AGGGTAGTGG GCAGCAGAGT 
    AACCAGGCTG GTCAGAGAAA CTCCACCAGT AGCATCGAGC CGAGGCTGCT CTGGGAGAGA 
    GTGGAGAAGC TGGCGCCCAG GCCGGGCAGT GGCAGCTCCT CGGGGTCCAG CAACTCGGGG 
    TCCCAGCCCG GCTCCCACCC AGGGTCTCAG AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA 
    TCATCATCCA AATCTGAAGG CTCGCCATCT CAGCGCCTTG AAAACGCAGC AAAAAAGCCT 
    GAAGATAAAA AGGAAGTGTT TAGACCTCTC AAGCCTGCTG GTGAAGTGGA CCTGACGGCT 
    CTGGCCAAAG AGCTGCGGGC CGTGGAGGAT GTGCGGCCGC CGCACAAGGT GACAGACTAC 
    TCCTCCTCCA GCGAGGAGTC GGGGACAACG GACGAGGAGG ACGACGACGT GGAGCAGGAG 
    GGGGCCGAGG AGCCTACGTC TGGCCCGGAG GACACCAGAG CTGCATCATC TCTGAACTTG 
    AGCAATGGTG AAACAGAGTC CGTGAAAACC ATGGTTGTCC ATGACGACGT GGAGAGTGAA 
    CCAGCCCTGA CCCCGTCCAA AGAGGGCACG CTGATCGTCC GCCAGAGTGC AGTTGACCAA 
    AAGCGCGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT 
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCTTCCCC ATCCTCCAGC 
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTAC TAATGAGACT 
    CAGTCCGCTA GTAACACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGGACAA CAGTGACTTC TGTGGTGGGG 
    TTTTCCTGTG ATGGAATGAG ACCTGAGGCC ATGAGGCAAG ATCCTACGCG GAAGGGCTCA 
    GTGGTCAATG TCAATCCCAC CAATACTCGG CCCCAGAGCG ACACCCCAGA GATTCGCAAA 
    TACAAGAAGA GGTTTAACTC CGAGATCCTG TGCGCAGCCT TGTGGGGAGT GAACTTGTTG 
    GTGGGTACCG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTGTATCCT 
    CTGATCAACA GAAGGCGATT TCAGCAGATG GACGTCCTGG AAGGCTTGAA CGTCTTAGTG 
    ACGATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
    ATACTTCACA ACGACCCGGA GGTTGAGAAG AAGCAGGGCT GGACGACAGT GGGGGACCTG 
    GAAGGCTGTG TGCATTATAA AGTTGTAAAA TATGAAAGGA TCAAATTTCT GGTCATTGCT 
    TTGAAGAACT CTGTGGAAGT CTACGCATGG GCACCCAAGC CATATCACAA ATTCATGGCC 
    TTTAAGTCAT TTGGTGAGCT GGTGCACAAG CCGTTGCTGG TGGATCTCAC TGTTGAGGAA 
    GGCCAGAGGC TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAC 
    TCAGGATCGG TCTATGACAT TTACCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG 
    ATCCAATGTA GCATCAAACC CCACGCAATC ATCATCCTCC CCAACACAGA TGGCATGGAG 
    CTCCTGGTGT GCTATGAAGA TGAAGGGGTT TACGTGAACA CGTACGGGAG GATCACCAAG 
    GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACGTCTGTAG CATATATTCG ATCCAATCAG 
    ACGATGGGCT GGGGAGAAAA GGCCATAGAG ATCCGATCTG TGGAAACTGG CCACTTGGAT 
    GGCGTGTTTA TGCATAAAAG GGCTCAAAGG CTGAAGTTCT TGTGTGAACG TAATGACAAG 
    GTGTTCTTTG CCTCCGTCCG GTCTGGGGGC AGCAGCCAGG TTTACTTCAT GACTCTCGGC 
    AGGACCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999331.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855099.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.
    Price & Availability↑
    CloneID OBa220754
    Clone ID Related Accession (Same CDS sequence) XM_024999330.1 , XM_024999331.1
    Accession Version XM_024999331.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTTA CCGAGGATGA AGAGGAGGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACGTAT TACGGTGCTT TCATTAAAAA GAGCCCCCCA 
    GGACACGATG ACCAGCTTTG GCTCGTTATG GAGTTCTGTG GGGCCGGATC CATCACGGAC 
    CTGGTGAAGA ACACCAAGGG CAACACGCTA AAGGAAGACT GGATAGCCTA CATCTCCAGG 
    GAGATCCTTC GGGGCCTGGC CCATCTCCAC ATCCATCACG TGATCCACCG CGACATCAAG 
    GGCCAGAATG TGCTGCTGAC CGAGAACGCA GAGGTGAAGC TGGTGGACTT CGGGGTGAGT 
    GCCCAGCTGG ACAGGACCGT GGGGAGGAGG AACACGTTCA TAGGCACCCC GTACTGGATG 
    GCCCCTGAGG TCATTGCCTG TGACGAGAAC CCCGACGCCA CCTACGATTA CAGGAGTGAC 
    CTCTGGTCCT GTGGCATCAC TGCCATTGAG ATGGCTGAAG GCGCTCCCCC TCTCTGCGAC 
    ATGCATCCCA TGAGAGCCCT GTTTCTCATC CCCCGGAATC CTCCTCCCCG GCTGAAGTCC 
    AAAAAATGGT CCAAGAAGTT TTTTAGTTTT ATAGAAGGAT GCCTGGTGAA GAATTACATG 
    CAGCGGCCCT CCACCGAGCA GCTTCTGAAG CATCCTTTCA TTAGGGATCA GCCGAACGAA 
    AGGCAGGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAAA CCGAGTATGA GTACAGTGGG AGTGAGGAAG AGGAGGAGGA GGCGCCTGAG 
    CAGGAGGGGG AGCCCAGCTC CATCGTCAAC GTGCCGGGGG AGTCGACGCT GCGCCGGGAC 
    TTCCTGCGGC TCCAGCAGGA GAACAAGGAG CGCTCTGAGG CCCTGAGGAG GCAGCAGCTC 
    CTGCAGGAGC AGCAGCTCCG CGAGCAGGAG GAGTATAAAC GGCAGCTGCT GGCGGAGAGG 
    CAGAAGCGGA TCGAGCAGCA GAAGGAGCAG AGGCGGCGGC TGGAAGAGCA ACAGAGGAGA 
    GAGCGGGAAG CCAGAAGGCA GCAGGAACGT GAGCAGCGGA GGCGGGAACA GGAGGAGAAG 
    AGGCGGCTGG AGGAGCTGGA GAGACGACGG AAGGAGGAGG AGGAGCGGAG GCGGGCGGAG 
    GAGGAGAAGA GGAGAGTGGA GAGGGAGCAG GAGTATATCC GACGGCAGCT GGAAGAGGAA 
    CAGCGTCACC TGGAACTGCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GCTACTGGAG 
    TGTCGCTGGC GGGAGCTGGA GGAGCACCGG CAGGCAGAGC GGCTCCAGAG GCAGCTGCAG 
    CAAGAACAAG CCTATCTCCT GTCTCTACAG CACGACCCCC GGAGGCCGCC CCCGCCGCCA 
    GACAGGAGCA AGGCTGGCCT GCACGAGCCC AAGGCCCCGC ACGAGCCCGC TGACCGGGCC 
    CGTGAGGTGG AAGATAGATT TAGGAAAACT AACCACAGCT CCCCCGAAGC CCAGTCTAAG 
    CAGACGGGCC GAGTGTTGGA ACCTCCAGTG CCTTCCAGAT CAGAGTCTTT TTCGAGTGGC 
    AACTCCGAGT CTGTGCACCC TGCCCTGCAG AGACCCGCCG AGCCACAGGT ACAGTGGTCC 
    CACCTGGCAT CTCTCAAGAA CAATGTTTCC CCTGTCTCGA GATCCCATTC CTTCAGTGAC 
    CCTTCTCCTC CCAAATTTGC ACACCACCAT CTTCGCTCTC AGGACCCCTG CCCACCTTCC 
    CGCAGTGAGG TGCTCAGTCA GAGTTCTGAC TCTAAGGCGG AGGTGCCCGA CCCAGCCCAG 
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAC GGGTCCCTGT GAGAACAACA 
    TCACGGTCCC CTGTCCTGTC CCGCCGGGAT TCCCCACTGC AGGGTAGTGG GCAGCAGAGT 
    AACCAGGCTG GTCAGAGAAA CTCCACCAGT AGCATCGAGC CGAGGCTGCT CTGGGAGAGA 
    GTGGAGAAGC TGGCGCCCAG GCCGGGCAGT GGCAGCTCCT CGGGGTCCAG CAACTCGGGG 
    TCCCAGCCCG GCTCCCACCC AGGGTCTCAG AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA 
    TCATCATCCA AATCTGAAGG CTCGCCATCT CAGCGCCTTG AAAACGCAGC AAAAAAGCCT 
    GAAGATAAAA AGGAAGTGTT TAGACCTCTC AAGCCTGCTG GTGAAGTGGA CCTGACGGCT 
    CTGGCCAAAG AGCTGCGGGC CGTGGAGGAT GTGCGGCCGC CGCACAAGGT GACAGACTAC 
    TCCTCCTCCA GCGAGGAGTC GGGGACAACG GACGAGGAGG ACGACGACGT GGAGCAGGAG 
    GGGGCCGAGG AGCCTACGTC TGGCCCGGAG GACACCAGAG CTGCATCATC TCTGAACTTG 
    AGCAATGGTG AAACAGAGTC CGTGAAAACC ATGGTTGTCC ATGACGACGT GGAGAGTGAA 
    CCAGCCCTGA CCCCGTCCAA AGAGGGCACG CTGATCGTCC GCCAGAGTGC AGTTGACCAA 
    AAGCGCGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT 
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCTTCCCC ATCCTCCAGC 
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTAC TAATGAGACT 
    CAGTCCGCTA GTAACACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGGACAA CAGTGACTTC TGTGGTGGGG 
    TTTTCCTGTG ATGGAATGAG ACCTGAGGCC ATGAGGCAAG ATCCTACGCG GAAGGGCTCA 
    GTGGTCAATG TCAATCCCAC CAATACTCGG CCCCAGAGCG ACACCCCAGA GATTCGCAAA 
    TACAAGAAGA GGTTTAACTC CGAGATCCTG TGCGCAGCCT TGTGGGGAGT GAACTTGTTG 
    GTGGGTACCG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTGTATCCT 
    CTGATCAACA GAAGGCGATT TCAGCAGATG GACGTCCTGG AAGGCTTGAA CGTCTTAGTG 
    ACGATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
    ATACTTCACA ACGACCCGGA GGTTGAGAAG AAGCAGGGCT GGACGACAGT GGGGGACCTG 
    GAAGGCTGTG TGCATTATAA AGTTGTAAAA TATGAAAGGA TCAAATTTCT GGTCATTGCT 
    TTGAAGAACT CTGTGGAAGT CTACGCATGG GCACCCAAGC CATATCACAA ATTCATGGCC 
    TTTAAGTCAT TTGGTGAGCT GGTGCACAAG CCGTTGCTGG TGGATCTCAC TGTTGAGGAA 
    GGCCAGAGGC TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAC 
    TCAGGATCGG TCTATGACAT TTACCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG 
    ATCCAATGTA GCATCAAACC CCACGCAATC ATCATCCTCC CCAACACAGA TGGCATGGAG 
    CTCCTGGTGT GCTATGAAGA TGAAGGGGTT TACGTGAACA CGTACGGGAG GATCACCAAG 
    GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACGTCTGTAG CATATATTCG ATCCAATCAG 
    ACGATGGGCT GGGGAGAAAA GGCCATAGAG ATCCGATCTG TGGAAACTGG CCACTTGGAT 
    GGCGTGTTTA TGCATAAAAG GGCTCAAAGG CTGAAGTTCT TGTGTGAACG TAATGACAAG 
    GTGTTCTTTG CCTCCGTCCG GTCTGGGGGC AGCAGCCAGG TTTACTTCAT GACTCTCGGC 
    AGGACCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855099.1
    CDS62..4045
    Translation

    Target ORF information:

    RefSeq Version XM_024999331.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999331.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTTA CCGAGGATGA AGAGGAGGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACGTAT TACGGTGCTT TCATTAAAAA GAGCCCCCCA 
    GGACACGATG ACCAGCTTTG GCTCGTTATG GAGTTCTGTG GGGCCGGATC CATCACGGAC 
    CTGGTGAAGA ACACCAAGGG CAACACGCTA AAGGAAGACT GGATAGCCTA CATCTCCAGG 
    GAGATCCTTC GGGGCCTGGC CCATCTCCAC ATCCATCACG TGATCCACCG CGACATCAAG 
    GGCCAGAATG TGCTGCTGAC CGAGAACGCA GAGGTGAAGC TGGTGGACTT CGGGGTGAGT 
    GCCCAGCTGG ACAGGACCGT GGGGAGGAGG AACACGTTCA TAGGCACCCC GTACTGGATG 
    GCCCCTGAGG TCATTGCCTG TGACGAGAAC CCCGACGCCA CCTACGATTA CAGGAGTGAC 
    CTCTGGTCCT GTGGCATCAC TGCCATTGAG ATGGCTGAAG GCGCTCCCCC TCTCTGCGAC 
    ATGCATCCCA TGAGAGCCCT GTTTCTCATC CCCCGGAATC CTCCTCCCCG GCTGAAGTCC 
    AAAAAATGGT CCAAGAAGTT TTTTAGTTTT ATAGAAGGAT GCCTGGTGAA GAATTACATG 
    CAGCGGCCCT CCACCGAGCA GCTTCTGAAG CATCCTTTCA TTAGGGATCA GCCGAACGAA 
    AGGCAGGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAAA CCGAGTATGA GTACAGTGGG AGTGAGGAAG AGGAGGAGGA GGCGCCTGAG 
    CAGGAGGGGG AGCCCAGCTC CATCGTCAAC GTGCCGGGGG AGTCGACGCT GCGCCGGGAC 
    TTCCTGCGGC TCCAGCAGGA GAACAAGGAG CGCTCTGAGG CCCTGAGGAG GCAGCAGCTC 
    CTGCAGGAGC AGCAGCTCCG CGAGCAGGAG GAGTATAAAC GGCAGCTGCT GGCGGAGAGG 
    CAGAAGCGGA TCGAGCAGCA GAAGGAGCAG AGGCGGCGGC TGGAAGAGCA ACAGAGGAGA 
    GAGCGGGAAG CCAGAAGGCA GCAGGAACGT GAGCAGCGGA GGCGGGAACA GGAGGAGAAG 
    AGGCGGCTGG AGGAGCTGGA GAGACGACGG AAGGAGGAGG AGGAGCGGAG GCGGGCGGAG 
    GAGGAGAAGA GGAGAGTGGA GAGGGAGCAG GAGTATATCC GACGGCAGCT GGAAGAGGAA 
    CAGCGTCACC TGGAACTGCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GCTACTGGAG 
    TGTCGCTGGC GGGAGCTGGA GGAGCACCGG CAGGCAGAGC GGCTCCAGAG GCAGCTGCAG 
    CAAGAACAAG CCTATCTCCT GTCTCTACAG CACGACCCCC GGAGGCCGCC CCCGCCGCCA 
    GACAGGAGCA AGGCTGGCCT GCACGAGCCC AAGGCCCCGC ACGAGCCCGC TGACCGGGCC 
    CGTGAGGTGG AAGATAGATT TAGGAAAACT AACCACAGCT CCCCCGAAGC CCAGTCTAAG 
    CAGACGGGCC GAGTGTTGGA ACCTCCAGTG CCTTCCAGAT CAGAGTCTTT TTCGAGTGGC 
    AACTCCGAGT CTGTGCACCC TGCCCTGCAG AGACCCGCCG AGCCACAGGT ACAGTGGTCC 
    CACCTGGCAT CTCTCAAGAA CAATGTTTCC CCTGTCTCGA GATCCCATTC CTTCAGTGAC 
    CCTTCTCCTC CCAAATTTGC ACACCACCAT CTTCGCTCTC AGGACCCCTG CCCACCTTCC 
    CGCAGTGAGG TGCTCAGTCA GAGTTCTGAC TCTAAGGCGG AGGTGCCCGA CCCAGCCCAG 
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAC GGGTCCCTGT GAGAACAACA 
    TCACGGTCCC CTGTCCTGTC CCGCCGGGAT TCCCCACTGC AGGGTAGTGG GCAGCAGAGT 
    AACCAGGCTG GTCAGAGAAA CTCCACCAGT AGCATCGAGC CGAGGCTGCT CTGGGAGAGA 
    GTGGAGAAGC TGGCGCCCAG GCCGGGCAGT GGCAGCTCCT CGGGGTCCAG CAACTCGGGG 
    TCCCAGCCCG GCTCCCACCC AGGGTCTCAG AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA 
    TCATCATCCA AATCTGAAGG CTCGCCATCT CAGCGCCTTG AAAACGCAGC AAAAAAGCCT 
    GAAGATAAAA AGGAAGTGTT TAGACCTCTC AAGCCTGCTG GTGAAGTGGA CCTGACGGCT 
    CTGGCCAAAG AGCTGCGGGC CGTGGAGGAT GTGCGGCCGC CGCACAAGGT GACAGACTAC 
    TCCTCCTCCA GCGAGGAGTC GGGGACAACG GACGAGGAGG ACGACGACGT GGAGCAGGAG 
    GGGGCCGAGG AGCCTACGTC TGGCCCGGAG GACACCAGAG CTGCATCATC TCTGAACTTG 
    AGCAATGGTG AAACAGAGTC CGTGAAAACC ATGGTTGTCC ATGACGACGT GGAGAGTGAA 
    CCAGCCCTGA CCCCGTCCAA AGAGGGCACG CTGATCGTCC GCCAGAGTGC AGTTGACCAA 
    AAGCGCGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT 
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCTTCCCC ATCCTCCAGC 
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTAC TAATGAGACT 
    CAGTCCGCTA GTAACACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGGACAA CAGTGACTTC TGTGGTGGGG 
    TTTTCCTGTG ATGGAATGAG ACCTGAGGCC ATGAGGCAAG ATCCTACGCG GAAGGGCTCA 
    GTGGTCAATG TCAATCCCAC CAATACTCGG CCCCAGAGCG ACACCCCAGA GATTCGCAAA 
    TACAAGAAGA GGTTTAACTC CGAGATCCTG TGCGCAGCCT TGTGGGGAGT GAACTTGTTG 
    GTGGGTACCG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTGTATCCT 
    CTGATCAACA GAAGGCGATT TCAGCAGATG GACGTCCTGG AAGGCTTGAA CGTCTTAGTG 
    ACGATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
    ATACTTCACA ACGACCCGGA GGTTGAGAAG AAGCAGGGCT GGACGACAGT GGGGGACCTG 
    GAAGGCTGTG TGCATTATAA AGTTGTAAAA TATGAAAGGA TCAAATTTCT GGTCATTGCT 
    TTGAAGAACT CTGTGGAAGT CTACGCATGG GCACCCAAGC CATATCACAA ATTCATGGCC 
    TTTAAGTCAT TTGGTGAGCT GGTGCACAAG CCGTTGCTGG TGGATCTCAC TGTTGAGGAA 
    GGCCAGAGGC TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAC 
    TCAGGATCGG TCTATGACAT TTACCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG 
    ATCCAATGTA GCATCAAACC CCACGCAATC ATCATCCTCC CCAACACAGA TGGCATGGAG 
    CTCCTGGTGT GCTATGAAGA TGAAGGGGTT TACGTGAACA CGTACGGGAG GATCACCAAG 
    GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACGTCTGTAG CATATATTCG ATCCAATCAG 
    ACGATGGGCT GGGGAGAAAA GGCCATAGAG ATCCGATCTG TGGAAACTGG CCACTTGGAT 
    GGCGTGTTTA TGCATAAAAG GGCTCAAAGG CTGAAGTTCT TGTGTGAACG TAATGACAAG 
    GTGTTCTTTG CCTCCGTCCG GTCTGGGGGC AGCAGCCAGG TTTACTTCAT GACTCTCGGC 
    AGGACCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999332.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855100.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.
    Price & Availability↑
    CloneID OBa220755
    Clone ID Related Accession (Same CDS sequence) XM_024999332.1
    Accession Version XM_024999332.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3981bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GTGGTCCCAC 
    CTGGCATCTC TCAAGAACAA TGTTTCCCCT GTCTCGAGAT CCCATTCCTT CAGTGACCCT 
    TCTCCTCCCA AATTTGCACA CCACCATCTT CGCTCTCAGG ACCCCTGCCC ACCTTCCCGC 
    AGTGAGGTGC TCAGTCAGAG TTCTGACTCT AAGGCGGAGG TGCCCGACCC AGCCCAGAAG 
    GCTTGGTCTA GATCAGACAG TGACGAGGTG CCTCCACGGG TCCCTGTGAG AACAACATCA 
    CGGTCCCCTG TCCTGTCCCG CCGGGATTCC CCACTGCAGG GTAGTGGGCA GCAGAGTAAC 
    CAGGCTGGTC AGAGAAACTC CACCAGTAGC ATCGAGCCGA GGCTGCTCTG GGAGAGAGTG 
    GAGAAGCTGG CGCCCAGGCC GGGCAGTGGC AGCTCCTCGG GGTCCAGCAA CTCGGGGTCC 
    CAGCCCGGCT CCCACCCAGG GTCTCAGAGT GGCTCCGGGG AGCGCTTCAG AGTGAGATCA 
    TCATCCAAAT CTGAAGGCTC GCCATCTCAG CGCCTTGAAA ACGCAGCAAA AAAGCCTGAA 
    GATAAAAAGG AAGTGTTTAG ACCTCTCAAG CCTGCTGGTG AAGTGGACCT GACGGCTCTG 
    GCCAAAGAGC TGCGGGCCGT GGAGGATGTG CGGCCGCCGC ACAAGGTGAC AGACTACTCC 
    TCCTCCAGCG AGGAGTCGGG GACAACGGAC GAGGAGGACG ACGACGTGGA GCAGGAGGGG 
    GCCGAGGAGC CTACGTCTGG CCCGGAGGAC ACCAGAGCTG CATCATCTCT GAACTTGAGC 
    AATGGTGAAA CAGAGTCCGT GAAAACCATG GTTGTCCATG ACGACGTGGA GAGTGAACCA 
    GCCCTGACCC CGTCCAAAGA GGGCACGCTG ATCGTCCGCC AGAGTGCAGT TGACCAAAAG 
    CGCGCCAGCC ATCATGAGAG CAATGGCTTT GCGGGTCGCA TTCACCTCTT GCCAGATCTC 
    TTACAGCAAA GCCATTCCTC CTCCACTTCC TCCACCTCCT CTTCCCCATC CTCCAGCCAG 
    CCGACACCCA CCATGTCCCC ACAGACACCC CAGGACAAGC TCACTACTAA TGAGACTCAG 
    TCCGCTAGTA ACACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
    CCCAGATTAC TACAGATTTC TCCATCTAGC GGGACAACAG TGACTTCTGT GGTGGGGTTT 
    TCCTGTGATG GAATGAGACC TGAGGCCATG AGGCAAGATC CTACGCGGAA GGGCTCAGTG 
    GTCAATGTCA ATCCCACCAA TACTCGGCCC CAGAGCGACA CCCCAGAGAT TCGCAAATAC 
    AAGAAGAGGT TTAACTCCGA GATCCTGTGC GCAGCCTTGT GGGGAGTGAA CTTGTTGGTG 
    GGTACCGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT GTATCCTCTG 
    ATCAACAGAA GGCGATTTCA GCAGATGGAC GTCCTGGAAG GCTTGAACGT CTTAGTGACG 
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA 
    CTTCACAACG ACCCGGAGGT TGAGAAGAAG CAGGGCTGGA CGACAGTGGG GGACCTGGAA 
    GGCTGTGTGC ATTATAAAGT TGTAAAATAT GAAAGGATCA AATTTCTGGT CATTGCTTTG 
    AAGAACTCTG TGGAAGTCTA CGCATGGGCA CCCAAGCCAT ATCACAAATT CATGGCCTTT 
    AAGTCATTTG GTGAGCTGGT GCACAAGCCG TTGCTGGTGG ATCTCACTGT TGAGGAAGGC 
    CAGAGGCTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGACTCA 
    GGATCGGTCT ATGACATTTA CCTACCAACA CACGTAAGAA AGAACCCACA CTCTATGATC 
    CAATGTAGCA TCAAACCCCA CGCAATCATC ATCCTCCCCA ACACAGATGG CATGGAGCTC 
    CTGGTGTGCT ATGAAGATGA AGGGGTTTAC GTGAACACGT ACGGGAGGAT CACCAAGGAC 
    GTGGTTCTGC AGTGGGGAGA GATGCCAACG TCTGTAGCAT ATATTCGATC CAATCAGACG 
    ATGGGCTGGG GAGAAAAGGC CATAGAGATC CGATCTGTGG AAACTGGCCA CTTGGATGGC 
    GTGTTTATGC ATAAAAGGGC TCAAAGGCTG AAGTTCTTGT GTGAACGTAA TGACAAGGTG 
    TTCTTTGCCT CCGTCCGGTC TGGGGGCAGC AGCCAGGTTT ACTTCATGAC TCTCGGCAGG 
    ACCTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855100.1
    CDS121..4101
    Translation

    Target ORF information:

    RefSeq Version XM_024999332.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999332.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GTGGTCCCAC 
    CTGGCATCTC TCAAGAACAA TGTTTCCCCT GTCTCGAGAT CCCATTCCTT CAGTGACCCT 
    TCTCCTCCCA AATTTGCACA CCACCATCTT CGCTCTCAGG ACCCCTGCCC ACCTTCCCGC 
    AGTGAGGTGC TCAGTCAGAG TTCTGACTCT AAGGCGGAGG TGCCCGACCC AGCCCAGAAG 
    GCTTGGTCTA GATCAGACAG TGACGAGGTG CCTCCACGGG TCCCTGTGAG AACAACATCA 
    CGGTCCCCTG TCCTGTCCCG CCGGGATTCC CCACTGCAGG GTAGTGGGCA GCAGAGTAAC 
    CAGGCTGGTC AGAGAAACTC CACCAGTAGC ATCGAGCCGA GGCTGCTCTG GGAGAGAGTG 
    GAGAAGCTGG CGCCCAGGCC GGGCAGTGGC AGCTCCTCGG GGTCCAGCAA CTCGGGGTCC 
    CAGCCCGGCT CCCACCCAGG GTCTCAGAGT GGCTCCGGGG AGCGCTTCAG AGTGAGATCA 
    TCATCCAAAT CTGAAGGCTC GCCATCTCAG CGCCTTGAAA ACGCAGCAAA AAAGCCTGAA 
    GATAAAAAGG AAGTGTTTAG ACCTCTCAAG CCTGCTGGTG AAGTGGACCT GACGGCTCTG 
    GCCAAAGAGC TGCGGGCCGT GGAGGATGTG CGGCCGCCGC ACAAGGTGAC AGACTACTCC 
    TCCTCCAGCG AGGAGTCGGG GACAACGGAC GAGGAGGACG ACGACGTGGA GCAGGAGGGG 
    GCCGAGGAGC CTACGTCTGG CCCGGAGGAC ACCAGAGCTG CATCATCTCT GAACTTGAGC 
    AATGGTGAAA CAGAGTCCGT GAAAACCATG GTTGTCCATG ACGACGTGGA GAGTGAACCA 
    GCCCTGACCC CGTCCAAAGA GGGCACGCTG ATCGTCCGCC AGAGTGCAGT TGACCAAAAG 
    CGCGCCAGCC ATCATGAGAG CAATGGCTTT GCGGGTCGCA TTCACCTCTT GCCAGATCTC 
    TTACAGCAAA GCCATTCCTC CTCCACTTCC TCCACCTCCT CTTCCCCATC CTCCAGCCAG 
    CCGACACCCA CCATGTCCCC ACAGACACCC CAGGACAAGC TCACTACTAA TGAGACTCAG 
    TCCGCTAGTA ACACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
    CCCAGATTAC TACAGATTTC TCCATCTAGC GGGACAACAG TGACTTCTGT GGTGGGGTTT 
    TCCTGTGATG GAATGAGACC TGAGGCCATG AGGCAAGATC CTACGCGGAA GGGCTCAGTG 
    GTCAATGTCA ATCCCACCAA TACTCGGCCC CAGAGCGACA CCCCAGAGAT TCGCAAATAC 
    AAGAAGAGGT TTAACTCCGA GATCCTGTGC GCAGCCTTGT GGGGAGTGAA CTTGTTGGTG 
    GGTACCGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT GTATCCTCTG 
    ATCAACAGAA GGCGATTTCA GCAGATGGAC GTCCTGGAAG GCTTGAACGT CTTAGTGACG 
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA 
    CTTCACAACG ACCCGGAGGT TGAGAAGAAG CAGGGCTGGA CGACAGTGGG GGACCTGGAA 
    GGCTGTGTGC ATTATAAAGT TGTAAAATAT GAAAGGATCA AATTTCTGGT CATTGCTTTG 
    AAGAACTCTG TGGAAGTCTA CGCATGGGCA CCCAAGCCAT ATCACAAATT CATGGCCTTT 
    AAGTCATTTG GTGAGCTGGT GCACAAGCCG TTGCTGGTGG ATCTCACTGT TGAGGAAGGC 
    CAGAGGCTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGACTCA 
    GGATCGGTCT ATGACATTTA CCTACCAACA CACGTAAGAA AGAACCCACA CTCTATGATC 
    CAATGTAGCA TCAAACCCCA CGCAATCATC ATCCTCCCCA ACACAGATGG CATGGAGCTC 
    CTGGTGTGCT ATGAAGATGA AGGGGTTTAC GTGAACACGT ACGGGAGGAT CACCAAGGAC 
    GTGGTTCTGC AGTGGGGAGA GATGCCAACG TCTGTAGCAT ATATTCGATC CAATCAGACG 
    ATGGGCTGGG GAGAAAAGGC CATAGAGATC CGATCTGTGG AAACTGGCCA CTTGGATGGC 
    GTGTTTATGC ATAAAAGGGC TCAAAGGCTG AAGTTCTTGT GTGAACGTAA TGACAAGGTG 
    TTCTTTGCCT CCGTCCGGTC TGGGGGCAGC AGCCAGGTTT ACTTCATGAC TCTCGGCAGG 
    ACCTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999333.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855101.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA.
    Price & Availability↑
    CloneID OBa220756
    Clone ID Related Accession (Same CDS sequence) XM_024999333.1
    Accession Version XM_024999333.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTACAGTGG 
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGAGATCCCA TTCCTTCAGT 
    GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGCT CTCAGGACCC CTGCCCACCT 
    TCCCGCAGTG AGGTGCTCAG TCAGAGTTCT GACTCTAAGG CGGAGGTGCC CGACCCAGCC 
    CAGAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CACGGGTCCC TGTGAGAACA 
    ACATCACGGT CCCCTGTCCT GTCCCGCCGG GATTCCCCAC TGCAGGGTAG TGGGCAGCAG 
    AGTAACCAGG CTGGTCAGAG AAACTCCACC AGCATCGAGC CGAGGCTGCT CTGGGAGAGA 
    GTGGAGAAGC TGGCGCCCAG GCCGGGCAGT GGCAGCTCCT CGGGGTCCAG CAACTCGGGG 
    TCCCAGCCCG GCTCCCACCC AGGGTCTCAG AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA 
    TCATCATCCA AATCTGAAGG CTCGCCATCT CAGCGCCTTG AAAACGCAGC AAAAAAGCCT 
    GAAGATAAAA AGGAAGTGTT TAGACCTCTC AAGCCTGCTG ACCTGACGGC TCTGGCCAAA 
    GAGCTGCGGG CCGTGGAGGA TGTGCGGCCG CCGCACAAGG TGACAGACTA CTCCTCCTCC 
    AGCGAGGAGT CGGGGACAAC GGACGAGGAG GACGACGACG TGGAGCAGGA GGGGGCCGAG 
    GAGCCTACGT CTGGCCCGGA GGACACCAGA GCTGCATCAT CTCTGAACTT GAGCAATGGT 
    GAAACAGAGT CCGTGAAAAC CATGGTTGTC CATGACGACG TGGAGAGTGA ACCAGCCCTG 
    ACCCCGTCCA AAGAGGGCAC GCTGATCGTC CGCCAGAGTG CAGTTGACCA AAAGCGCGCC 
    AGCCATCATG AGAGCAATGG CTTTGCGGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG 
    CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCTTCCC CATCCTCCAG CCAGCCGACA 
    CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTA CTAATGAGAC TCAGTCCGCT 
    AGTAACACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
    TTACTACAGA TTTCTCCATC TAGCGGGACA ACAGTGACTT CTGTGGTGGG GTTTTCCTGT 
    GATGGAATGA GACCTGAGGC CATGAGGCAA GATCCTACGC GGAAGGGCTC AGTGGTCAAT 
    GTCAATCCCA CCAATACTCG GCCCCAGAGC GACACCCCAG AGATTCGCAA ATACAAGAAG 
    AGGTTTAACT CCGAGATCCT GTGCGCAGCC TTGTGGGGAG TGAACTTGTT GGTGGGTACC 
    GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTGTATCC TCTGATCAAC 
    AGAAGGCGAT TTCAGCAGAT GGACGTCCTG GAAGGCTTGA ACGTCTTAGT GACGATATCT 
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
    AACGACCCGG AGGTTGAGAA GAAGCAGGGC TGGACGACAG TGGGGGACCT GGAAGGCTGT 
    GTGCATTATA AAGTTGTAAA ATATGAAAGG ATCAAATTTC TGGTCATTGC TTTGAAGAAC 
    TCTGTGGAAG TCTACGCATG GGCACCCAAG CCATATCACA AATTCATGGC CTTTAAGTCA 
    TTTGGTGAGC TGGTGCACAA GCCGTTGCTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
    CTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA CTCAGGATCG 
    GTCTATGACA TTTACCTACC AACACACGTA AGAAAGAACC CACACTCTAT GATCCAATGT 
    AGCATCAAAC CCCACGCAAT CATCATCCTC CCCAACACAG ATGGCATGGA GCTCCTGGTG 
    TGCTATGAAG ATGAAGGGGT TTACGTGAAC ACGTACGGGA GGATCACCAA GGACGTGGTT 
    CTGCAGTGGG GAGAGATGCC AACGTCTGTA GCATATATTC GATCCAATCA GACGATGGGC 
    TGGGGAGAAA AGGCCATAGA GATCCGATCT GTGGAAACTG GCCACTTGGA TGGCGTGTTT 
    ATGCATAAAA GGGCTCAAAG GCTGAAGTTC TTGTGTGAAC GTAATGACAA GGTGTTCTTT 
    GCCTCCGTCC GGTCTGGGGG CAGCAGCCAG GTTTACTTCA TGACTCTCGG CAGGACCTCT 
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855101.1
    CDS121..4095
    Translation

    Target ORF information:

    RefSeq Version XM_024999333.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999333.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTACAGTGG 
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGAGATCCCA TTCCTTCAGT 
    GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGCT CTCAGGACCC CTGCCCACCT 
    TCCCGCAGTG AGGTGCTCAG TCAGAGTTCT GACTCTAAGG CGGAGGTGCC CGACCCAGCC 
    CAGAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CACGGGTCCC TGTGAGAACA 
    ACATCACGGT CCCCTGTCCT GTCCCGCCGG GATTCCCCAC TGCAGGGTAG TGGGCAGCAG 
    AGTAACCAGG CTGGTCAGAG AAACTCCACC AGCATCGAGC CGAGGCTGCT CTGGGAGAGA 
    GTGGAGAAGC TGGCGCCCAG GCCGGGCAGT GGCAGCTCCT CGGGGTCCAG CAACTCGGGG 
    TCCCAGCCCG GCTCCCACCC AGGGTCTCAG AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA 
    TCATCATCCA AATCTGAAGG CTCGCCATCT CAGCGCCTTG AAAACGCAGC AAAAAAGCCT 
    GAAGATAAAA AGGAAGTGTT TAGACCTCTC AAGCCTGCTG ACCTGACGGC TCTGGCCAAA 
    GAGCTGCGGG CCGTGGAGGA TGTGCGGCCG CCGCACAAGG TGACAGACTA CTCCTCCTCC 
    AGCGAGGAGT CGGGGACAAC GGACGAGGAG GACGACGACG TGGAGCAGGA GGGGGCCGAG 
    GAGCCTACGT CTGGCCCGGA GGACACCAGA GCTGCATCAT CTCTGAACTT GAGCAATGGT 
    GAAACAGAGT CCGTGAAAAC CATGGTTGTC CATGACGACG TGGAGAGTGA ACCAGCCCTG 
    ACCCCGTCCA AAGAGGGCAC GCTGATCGTC CGCCAGAGTG CAGTTGACCA AAAGCGCGCC 
    AGCCATCATG AGAGCAATGG CTTTGCGGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG 
    CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCTTCCC CATCCTCCAG CCAGCCGACA 
    CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTA CTAATGAGAC TCAGTCCGCT 
    AGTAACACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
    TTACTACAGA TTTCTCCATC TAGCGGGACA ACAGTGACTT CTGTGGTGGG GTTTTCCTGT 
    GATGGAATGA GACCTGAGGC CATGAGGCAA GATCCTACGC GGAAGGGCTC AGTGGTCAAT 
    GTCAATCCCA CCAATACTCG GCCCCAGAGC GACACCCCAG AGATTCGCAA ATACAAGAAG 
    AGGTTTAACT CCGAGATCCT GTGCGCAGCC TTGTGGGGAG TGAACTTGTT GGTGGGTACC 
    GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTGTATCC TCTGATCAAC 
    AGAAGGCGAT TTCAGCAGAT GGACGTCCTG GAAGGCTTGA ACGTCTTAGT GACGATATCT 
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
    AACGACCCGG AGGTTGAGAA GAAGCAGGGC TGGACGACAG TGGGGGACCT GGAAGGCTGT 
    GTGCATTATA AAGTTGTAAA ATATGAAAGG ATCAAATTTC TGGTCATTGC TTTGAAGAAC 
    TCTGTGGAAG TCTACGCATG GGCACCCAAG CCATATCACA AATTCATGGC CTTTAAGTCA 
    TTTGGTGAGC TGGTGCACAA GCCGTTGCTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
    CTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA CTCAGGATCG 
    GTCTATGACA TTTACCTACC AACACACGTA AGAAAGAACC CACACTCTAT GATCCAATGT 
    AGCATCAAAC CCCACGCAAT CATCATCCTC CCCAACACAG ATGGCATGGA GCTCCTGGTG 
    TGCTATGAAG ATGAAGGGGT TTACGTGAAC ACGTACGGGA GGATCACCAA GGACGTGGTT 
    CTGCAGTGGG GAGAGATGCC AACGTCTGTA GCATATATTC GATCCAATCA GACGATGGGC 
    TGGGGAGAAA AGGCCATAGA GATCCGATCT GTGGAAACTG GCCACTTGGA TGGCGTGTTT 
    ATGCATAAAA GGGCTCAAAG GCTGAAGTTC TTGTGTGAAC GTAATGACAA GGTGTTCTTT 
    GCCTCCGTCC GGTCTGGGGG CAGCAGCCAG GTTTACTTCA TGACTCTCGG CAGGACCTCT 
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999334.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855102.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA.
    Price & Availability↑
    CloneID OBa220757
    Clone ID Related Accession (Same CDS sequence) XM_024999334.1
    Accession Version XM_024999334.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3957bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCACGGGTC CCTGTGAGAA CAACATCACG GTCCCCTGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGT AGTGGGCAGC AGAGTAACCA GGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCGAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCGG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CGGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCTCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGATGTGCG GCCGCCGCAC AAGGTGACAG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CAACGGACGA GGAGGACGAC GACGTGGAGC AGGAGGGGGC CGAGGAGCCT 
    ACGTCTGGCC CGGAGGACAC CAGAGCTGCA TCATCTCTGA ACTTGAGCAA TGGTGAAACA 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG ACTCAGTCCG CTAGTAACAC ACTCCAGAAA 
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
    TCTAGCGGGA CAACAGTGAC TTCTGTGGTG GGGTTTTCCT GTGATGGAAT GAGACCTGAG 
    GCCATGAGGC AAGATCCTAC GCGGAAGGGC TCAGTGGTCA ATGTCAATCC CACCAATACT 
    CGGCCCCAGA GCGACACCCC AGAGATTCGC AAATACAAGA AGAGGTTTAA CTCCGAGATC 
    CTGTGCGCAG CCTTGTGGGG AGTGAACTTG TTGGTGGGTA CCGAGAGTGG CCTGATGCTG 
    CTGGACAGAA GTGGCCAAGG GAAGGTGTAT CCTCTGATCA ACAGAAGGCG ATTTCAGCAG 
    ATGGACGTCC TGGAAGGCTT GAACGTCTTA GTGACGATAT CTGGCAAAAA GGATAAGTTA 
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAACGACCC GGAGGTTGAG 
    AAGAAGCAGG GCTGGACGAC AGTGGGGGAC CTGGAAGGCT GTGTGCATTA TAAAGTTGTA 
    AAATATGAAA GGATCAAATT TCTGGTCATT GCTTTGAAGA ACTCTGTGGA AGTCTACGCA 
    TGGGCACCCA AGCCATATCA CAAATTCATG GCCTTTAAGT CATTTGGTGA GCTGGTGCAC 
    AAGCCGTTGC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGCTGAAAGT GATCTATGGA 
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GACTCAGGAT CGGTCTATGA CATTTACCTA 
    CCAACACACG TAAGAAAGAA CCCACACTCT ATGATCCAAT GTAGCATCAA ACCCCACGCA 
    ATCATCATCC TCCCCAACAC AGATGGCATG GAGCTCCTGG TGTGCTATGA AGATGAAGGG 
    GTTTACGTGA ACACGTACGG GAGGATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG 
    CCAACGTCTG TAGCATATAT TCGATCCAAT CAGACGATGG GCTGGGGAGA AAAGGCCATA 
    GAGATCCGAT CTGTGGAAAC TGGCCACTTG GATGGCGTGT TTATGCATAA AAGGGCTCAA 
    AGGCTGAAGT TCTTGTGTGA ACGTAATGAC AAGGTGTTCT TTGCCTCCGT CCGGTCTGGG 
    GGCAGCAGCC AGGTTTACTT CATGACTCTC GGCAGGACCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855102.1
    CDS121..4077
    Translation

    Target ORF information:

    RefSeq Version XM_024999334.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999334.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCACGGGTC CCTGTGAGAA CAACATCACG GTCCCCTGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGT AGTGGGCAGC AGAGTAACCA GGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCGAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCGG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CGGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCTCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGATGTGCG GCCGCCGCAC AAGGTGACAG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CAACGGACGA GGAGGACGAC GACGTGGAGC AGGAGGGGGC CGAGGAGCCT 
    ACGTCTGGCC CGGAGGACAC CAGAGCTGCA TCATCTCTGA ACTTGAGCAA TGGTGAAACA 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG ACTCAGTCCG CTAGTAACAC ACTCCAGAAA 
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
    TCTAGCGGGA CAACAGTGAC TTCTGTGGTG GGGTTTTCCT GTGATGGAAT GAGACCTGAG 
    GCCATGAGGC AAGATCCTAC GCGGAAGGGC TCAGTGGTCA ATGTCAATCC CACCAATACT 
    CGGCCCCAGA GCGACACCCC AGAGATTCGC AAATACAAGA AGAGGTTTAA CTCCGAGATC 
    CTGTGCGCAG CCTTGTGGGG AGTGAACTTG TTGGTGGGTA CCGAGAGTGG CCTGATGCTG 
    CTGGACAGAA GTGGCCAAGG GAAGGTGTAT CCTCTGATCA ACAGAAGGCG ATTTCAGCAG 
    ATGGACGTCC TGGAAGGCTT GAACGTCTTA GTGACGATAT CTGGCAAAAA GGATAAGTTA 
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAACGACCC GGAGGTTGAG 
    AAGAAGCAGG GCTGGACGAC AGTGGGGGAC CTGGAAGGCT GTGTGCATTA TAAAGTTGTA 
    AAATATGAAA GGATCAAATT TCTGGTCATT GCTTTGAAGA ACTCTGTGGA AGTCTACGCA 
    TGGGCACCCA AGCCATATCA CAAATTCATG GCCTTTAAGT CATTTGGTGA GCTGGTGCAC 
    AAGCCGTTGC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGCTGAAAGT GATCTATGGA 
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GACTCAGGAT CGGTCTATGA CATTTACCTA 
    CCAACACACG TAAGAAAGAA CCCACACTCT ATGATCCAAT GTAGCATCAA ACCCCACGCA 
    ATCATCATCC TCCCCAACAC AGATGGCATG GAGCTCCTGG TGTGCTATGA AGATGAAGGG 
    GTTTACGTGA ACACGTACGG GAGGATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG 
    CCAACGTCTG TAGCATATAT TCGATCCAAT CAGACGATGG GCTGGGGAGA AAAGGCCATA 
    GAGATCCGAT CTGTGGAAAC TGGCCACTTG GATGGCGTGT TTATGCATAA AAGGGCTCAA 
    AGGCTGAAGT TCTTGTGTGA ACGTAATGAC AAGGTGTTCT TTGCCTCCGT CCGGTCTGGG 
    GGCAGCAGCC AGGTTTACTT CATGACTCTC GGCAGGACCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999335.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855103.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA.
    Price & Availability↑
    CloneID OBa220758
    Clone ID Related Accession (Same CDS sequence) XM_024999335.1
    Accession Version XM_024999335.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3948bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCACGGGTC CCTGTGAGAA CAACATCACG GTCCCCTGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGT AGTGGGCAGC AGAGTAACCA GGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCGAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCGG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CGGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGACCTG ACGGCTCTGG CCAAAGAGCT GCGGGCCGTG 
    GAGGATGTGC GGCCGCCGCA CAAGGTGACA GACTACTCCT CCTCCAGCGA GGAGTCGGGG 
    ACAACGGACG AGGAGGACGA CGACGTGGAG CAGGAGGGGG CCGAGGAGCC TACGTCTGGC 
    CCGGAGGACA CCAGAGCTGC ATCATCTCTG AACTTGAGCA ATGGTGAAAC AGAGTCCGTG 
    AAAACCATGG TTGTCCATGA CGACGTGGAG AGTGAACCAG CCCTGACCCC GTCCAAAGAG 
    GGCACGCTGA TCGTCCGCCA GACTCAGTCC GCTAGTAACA CACTCCAGAA ACACAAATCT 
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGCGGG 
    ACAACAGTGA CTTCTGTGGT GGGGTTTTCC TGTGATGGAA TGAGACCTGA GGCCATGAGG 
    CAAGATCCTA CGCGGAAGGG CTCAGTGGTC AATGTCAATC CCACCAATAC TCGGCCCCAG 
    AGCGACACCC CAGAGATTCG CAAATACAAG AAGAGGTTTA ACTCCGAGAT CCTGTGCGCA 
    GCCTTGTGGG GAGTGAACTT GTTGGTGGGT ACCGAGAGTG GCCTGATGCT GCTGGACAGA 
    AGTGGCCAAG GGAAGGTGTA TCCTCTGATC AACAGAAGGC GATTTCAGCA GATGGACGTC 
    CTGGAAGGCT TGAACGTCTT AGTGACGATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC 
    TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAACGACC CGGAGGTTGA GAAGAAGCAG 
    GGCTGGACGA CAGTGGGGGA CCTGGAAGGC TGTGTGCATT ATAAAGTTGT AAAATATGAA 
    AGGATCAAAT TTCTGGTCAT TGCTTTGAAG AACTCTGTGG AAGTCTACGC ATGGGCACCC 
    AAGCCATATC ACAAATTCAT GGCCTTTAAG TCATTTGGTG AGCTGGTGCA CAAGCCGTTG 
    CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGCTGAAAG TGATCTATGG ATCCTGTGCT 
    GGATTCCATG CTGTTGATGT GGACTCAGGA TCGGTCTATG ACATTTACCT ACCAACACAC 
    GTAAGAAAGA ACCCACACTC TATGATCCAA TGTAGCATCA AACCCCACGC AATCATCATC 
    CTCCCCAACA CAGATGGCAT GGAGCTCCTG GTGTGCTATG AAGATGAAGG GGTTTACGTG 
    AACACGTACG GGAGGATCAC CAAGGACGTG GTTCTGCAGT GGGGAGAGAT GCCAACGTCT 
    GTAGCATATA TTCGATCCAA TCAGACGATG GGCTGGGGAG AAAAGGCCAT AGAGATCCGA 
    TCTGTGGAAA CTGGCCACTT GGATGGCGTG TTTATGCATA AAAGGGCTCA AAGGCTGAAG 
    TTCTTGTGTG AACGTAATGA CAAGGTGTTC TTTGCCTCCG TCCGGTCTGG GGGCAGCAGC 
    CAGGTTTACT TCATGACTCT CGGCAGGACC TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855103.1
    CDS121..4068
    Translation

    Target ORF information:

    RefSeq Version XM_024999335.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999335.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCACGGGTC CCTGTGAGAA CAACATCACG GTCCCCTGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGT AGTGGGCAGC AGAGTAACCA GGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCGAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCGG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CGGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGACCTG ACGGCTCTGG CCAAAGAGCT GCGGGCCGTG 
    GAGGATGTGC GGCCGCCGCA CAAGGTGACA GACTACTCCT CCTCCAGCGA GGAGTCGGGG 
    ACAACGGACG AGGAGGACGA CGACGTGGAG CAGGAGGGGG CCGAGGAGCC TACGTCTGGC 
    CCGGAGGACA CCAGAGCTGC ATCATCTCTG AACTTGAGCA ATGGTGAAAC AGAGTCCGTG 
    AAAACCATGG TTGTCCATGA CGACGTGGAG AGTGAACCAG CCCTGACCCC GTCCAAAGAG 
    GGCACGCTGA TCGTCCGCCA GACTCAGTCC GCTAGTAACA CACTCCAGAA ACACAAATCT 
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGCGGG 
    ACAACAGTGA CTTCTGTGGT GGGGTTTTCC TGTGATGGAA TGAGACCTGA GGCCATGAGG 
    CAAGATCCTA CGCGGAAGGG CTCAGTGGTC AATGTCAATC CCACCAATAC TCGGCCCCAG 
    AGCGACACCC CAGAGATTCG CAAATACAAG AAGAGGTTTA ACTCCGAGAT CCTGTGCGCA 
    GCCTTGTGGG GAGTGAACTT GTTGGTGGGT ACCGAGAGTG GCCTGATGCT GCTGGACAGA 
    AGTGGCCAAG GGAAGGTGTA TCCTCTGATC AACAGAAGGC GATTTCAGCA GATGGACGTC 
    CTGGAAGGCT TGAACGTCTT AGTGACGATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC 
    TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAACGACC CGGAGGTTGA GAAGAAGCAG 
    GGCTGGACGA CAGTGGGGGA CCTGGAAGGC TGTGTGCATT ATAAAGTTGT AAAATATGAA 
    AGGATCAAAT TTCTGGTCAT TGCTTTGAAG AACTCTGTGG AAGTCTACGC ATGGGCACCC 
    AAGCCATATC ACAAATTCAT GGCCTTTAAG TCATTTGGTG AGCTGGTGCA CAAGCCGTTG 
    CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGCTGAAAG TGATCTATGG ATCCTGTGCT 
    GGATTCCATG CTGTTGATGT GGACTCAGGA TCGGTCTATG ACATTTACCT ACCAACACAC 
    GTAAGAAAGA ACCCACACTC TATGATCCAA TGTAGCATCA AACCCCACGC AATCATCATC 
    CTCCCCAACA CAGATGGCAT GGAGCTCCTG GTGTGCTATG AAGATGAAGG GGTTTACGTG 
    AACACGTACG GGAGGATCAC CAAGGACGTG GTTCTGCAGT GGGGAGAGAT GCCAACGTCT 
    GTAGCATATA TTCGATCCAA TCAGACGATG GGCTGGGGAG AAAAGGCCAT AGAGATCCGA 
    TCTGTGGAAA CTGGCCACTT GGATGGCGTG TTTATGCATA AAAGGGCTCA AAGGCTGAAG 
    TTCTTGTGTG AACGTAATGA CAAGGTGTTC TTTGCCTCCG TCCGGTCTGG GGGCAGCAGC 
    CAGGTTTACT TCATGACTCT CGGCAGGACC TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999336.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855104.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA.
    Price & Availability↑
    CloneID OBa220759
    Clone ID Related Accession (Same CDS sequence) XM_024999336.1
    Accession Version XM_024999336.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3933bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCACGGGTC CCTGTGAGAA CAACATCACG GTCCCCTGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGT AGTGGGCAGC AGAGTAACCA GGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCGAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCGG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CGGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCTCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGATGTGCG GCCGCCGCAC AAGGTGACAG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CAACGGACGA GGAGGACGAC GACGTGGAGC AGGAGGGGGC CGAGGAGCCT 
    ACGTCTGGCC CGGAGGACAC CAGAGCTGCA TCATCTCTGA ACTTGAGCAA TGGTGAAACA 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG ACTCAGTCCG CTAGTAACAC ACTCCAGAAA 
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
    TCTAGCGGGA CAACAGTGAC TTCTGTGGTG GGGTTTTCCT GTGATGGAAT GAGACCTGAG 
    GCCATGAGGC AAGATCCTAC GCGGAAGGGC TCAGTGGTCA ATGTCAATCC CACCAATACT 
    CGGCCCCAGA GCGACACCCC AGAGATTCGC AAATACAAGA AGAGGTTTAA CTCCGAGATC 
    CTGTGCGCAG CCTTGTGGGG AGTGAACTTG TTGGTGGGTA CCGAGAGTGG CCTGATGCTG 
    CTGGACAGAA GTGGCCAAGG GAAGGTGTAT CCTCTGATCA ACAGAAGGCG ATTTCAGCAG 
    ATGGACGTCC TGGAAGGCTT GAACGTCTTA GTGACGATAT CTGGCAAAAA GGATAAGTTA 
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAACGACCC GGAGGTTGAG 
    AAGAAGCAGG GCTGGACGAC AGTGGGGGAC CTGGAAGGCT GTGTGCATTA TAAAGTTGTA 
    AAATATGAAA GGATCAAATT TCTGGTCATT GCTTTGAAGA ACTCTGTGGA AGTCTACGCA 
    TGGGCACCCA AGCCATATCA CAAATTCATG GCCTTTAAGT CATTTGGTGA GCTGGTGCAC 
    AAGCCGTTGC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGCTGAAAGT GATCTATGGA 
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GACTCAGGAT CGGTCTATGA CATTTACCTA 
    CCAACACACA TCCAATGTAG CATCAAACCC CACGCAATCA TCATCCTCCC CAACACAGAT 
    GGCATGGAGC TCCTGGTGTG CTATGAAGAT GAAGGGGTTT ACGTGAACAC GTACGGGAGG 
    ATCACCAAGG ACGTGGTTCT GCAGTGGGGA GAGATGCCAA CGTCTGTAGC ATATATTCGA 
    TCCAATCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGATCTGT GGAAACTGGC 
    CACTTGGATG GCGTGTTTAT GCATAAAAGG GCTCAAAGGC TGAAGTTCTT GTGTGAACGT 
    AATGACAAGG TGTTCTTTGC CTCCGTCCGG TCTGGGGGCA GCAGCCAGGT TTACTTCATG 
    ACTCTCGGCA GGACCTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855104.1
    CDS121..4053
    Translation

    Target ORF information:

    RefSeq Version XM_024999336.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999336.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCACGGGTC CCTGTGAGAA CAACATCACG GTCCCCTGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGT AGTGGGCAGC AGAGTAACCA GGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCGAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCGG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CGGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCTCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGATGTGCG GCCGCCGCAC AAGGTGACAG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CAACGGACGA GGAGGACGAC GACGTGGAGC AGGAGGGGGC CGAGGAGCCT 
    ACGTCTGGCC CGGAGGACAC CAGAGCTGCA TCATCTCTGA ACTTGAGCAA TGGTGAAACA 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG ACTCAGTCCG CTAGTAACAC ACTCCAGAAA 
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
    TCTAGCGGGA CAACAGTGAC TTCTGTGGTG GGGTTTTCCT GTGATGGAAT GAGACCTGAG 
    GCCATGAGGC AAGATCCTAC GCGGAAGGGC TCAGTGGTCA ATGTCAATCC CACCAATACT 
    CGGCCCCAGA GCGACACCCC AGAGATTCGC AAATACAAGA AGAGGTTTAA CTCCGAGATC 
    CTGTGCGCAG CCTTGTGGGG AGTGAACTTG TTGGTGGGTA CCGAGAGTGG CCTGATGCTG 
    CTGGACAGAA GTGGCCAAGG GAAGGTGTAT CCTCTGATCA ACAGAAGGCG ATTTCAGCAG 
    ATGGACGTCC TGGAAGGCTT GAACGTCTTA GTGACGATAT CTGGCAAAAA GGATAAGTTA 
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAACGACCC GGAGGTTGAG 
    AAGAAGCAGG GCTGGACGAC AGTGGGGGAC CTGGAAGGCT GTGTGCATTA TAAAGTTGTA 
    AAATATGAAA GGATCAAATT TCTGGTCATT GCTTTGAAGA ACTCTGTGGA AGTCTACGCA 
    TGGGCACCCA AGCCATATCA CAAATTCATG GCCTTTAAGT CATTTGGTGA GCTGGTGCAC 
    AAGCCGTTGC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGCTGAAAGT GATCTATGGA 
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GACTCAGGAT CGGTCTATGA CATTTACCTA 
    CCAACACACA TCCAATGTAG CATCAAACCC CACGCAATCA TCATCCTCCC CAACACAGAT 
    GGCATGGAGC TCCTGGTGTG CTATGAAGAT GAAGGGGTTT ACGTGAACAC GTACGGGAGG 
    ATCACCAAGG ACGTGGTTCT GCAGTGGGGA GAGATGCCAA CGTCTGTAGC ATATATTCGA 
    TCCAATCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGATCTGT GGAAACTGGC 
    CACTTGGATG GCGTGTTTAT GCATAAAAGG GCTCAAAGGC TGAAGTTCTT GTGTGAACGT 
    AATGACAAGG TGTTCTTTGC CTCCGTCCGG TCTGGGGGCA GCAGCCAGGT TTACTTCATG 
    ACTCTCGGCA GGACCTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999337.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855105.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA.
    Price & Availability↑
    CloneID OBa220760
    Clone ID Related Accession (Same CDS sequence) XM_024999337.1
    Accession Version XM_024999337.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3915bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTCCCTG TGAGAACAAC ATCACGGTCC 
    CCTGTCCTGT CCCGCCGGGA TTCCCCACTG CAGGGTAGTG GGCAGCAGAG TAACCAGGCT 
    GGTCAGAGAA ACTCCACCAG TAGCATCGAG CCGAGGCTGC TCTGGGAGAG AGTGGAGAAG 
    CTGGCGCCCA GGCCGGGCAG TGGCAGCTCC TCGGGGTCCA GCAACTCGGG GTCCCAGCCC 
    GGCTCCCACC CAGGGTCTCA GAGTGGCTCC GGGGAGCGCT TCAGAGTGAG ATCATCATCC 
    AAATCTGAAG GCTCGCCATC TCAGCGCCTT GAAAACGCAG CAAAAAAGCC TGAAGATAAA 
    AAGGAAGTGT TTAGACCTCT CAAGCCTGCT GGTGAAGTGG ACCTGACGGC TCTGGCCAAA 
    GAGCTGCGGG CCGTGGAGGA TGTGCGGCCG CCGCACAAGG TGACAGACTA CTCCTCCTCC 
    AGCGAGGAGT CGGGGACAAC GGACGAGGAG GACGACGACG TGGAGCAGGA GGGGGCCGAG 
    GAGCCTACGT CTGGCCCGGA GGACACCAGA GCTGCATCAT CTCTGAACTT GAGCAATGGT 
    GAAACAGAGT CCGTGAAAAC CATGGTTGTC CATGACGACG TGGAGAGTGA ACCAGCCCTG 
    ACCCCGTCCA AAGAGGGCAC GCTGATCGTC CGCCAGAGTG CAGTTGACCA AAAGCGCGCC 
    AGCCATCATG AGAGCAATGG CTTTGCGGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG 
    CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCTTCCC CATCCTCCAG CCAGCCGACA 
    CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTA CTAATGAGAC TCAGTCCGCT 
    AGTAACACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
    TTACTACAGA TTTCTCCATC TAGCGGGACA ACAGTGACTT CTGTGGTGGG GTTTTCCTGT 
    GATGGAATGA GACCTGAGGC CATGAGGCAA GATCCTACGC GGAAGGGCTC AGTGGTCAAT 
    GTCAATCCCA CCAATACTCG GCCCCAGAGC GACACCCCAG AGATTCGCAA ATACAAGAAG 
    AGGTTTAACT CCGAGATCCT GTGCGCAGCC TTGTGGGGAG TGAACTTGTT GGTGGGTACC 
    GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTGTATCC TCTGATCAAC 
    AGAAGGCGAT TTCAGCAGAT GGACGTCCTG GAAGGCTTGA ACGTCTTAGT GACGATATCT 
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
    AACGACCCGG AGGTTGAGAA GAAGCAGGGC TGGACGACAG TGGGGGACCT GGAAGGCTGT 
    GTGCATTATA AAGTTGTAAA ATATGAAAGG ATCAAATTTC TGGTCATTGC TTTGAAGAAC 
    TCTGTGGAAG TCTACGCATG GGCACCCAAG CCATATCACA AATTCATGGC CTTTAAGTCA 
    TTTGGTGAGC TGGTGCACAA GCCGTTGCTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
    CTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA CTCAGGATCG 
    GTCTATGACA TTTACCTACC AACACACGTA AGAAAGAACC CACACTCTAT GATCCAATGT 
    AGCATCAAAC CCCACGCAAT CATCATCCTC CCCAACACAG ATGGCATGGA GCTCCTGGTG 
    TGCTATGAAG ATGAAGGGGT TTACGTGAAC ACGTACGGGA GGATCACCAA GGACGTGGTT 
    CTGCAGTGGG GAGAGATGCC AACGTCTGTA GCATATATTC GATCCAATCA GACGATGGGC 
    TGGGGAGAAA AGGCCATAGA GATCCGATCT GTGGAAACTG GCCACTTGGA TGGCGTGTTT 
    ATGCATAAAA GGGCTCAAAG GCTGAAGTTC TTGTGTGAAC GTAATGACAA GGTGTTCTTT 
    GCCTCCGTCC GGTCTGGGGG CAGCAGCCAG GTTTACTTCA TGACTCTCGG CAGGACCTCT 
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855105.1
    CDS121..4035
    Translation

    Target ORF information:

    RefSeq Version XM_024999337.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999337.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTCCCTG TGAGAACAAC ATCACGGTCC 
    CCTGTCCTGT CCCGCCGGGA TTCCCCACTG CAGGGTAGTG GGCAGCAGAG TAACCAGGCT 
    GGTCAGAGAA ACTCCACCAG TAGCATCGAG CCGAGGCTGC TCTGGGAGAG AGTGGAGAAG 
    CTGGCGCCCA GGCCGGGCAG TGGCAGCTCC TCGGGGTCCA GCAACTCGGG GTCCCAGCCC 
    GGCTCCCACC CAGGGTCTCA GAGTGGCTCC GGGGAGCGCT TCAGAGTGAG ATCATCATCC 
    AAATCTGAAG GCTCGCCATC TCAGCGCCTT GAAAACGCAG CAAAAAAGCC TGAAGATAAA 
    AAGGAAGTGT TTAGACCTCT CAAGCCTGCT GGTGAAGTGG ACCTGACGGC TCTGGCCAAA 
    GAGCTGCGGG CCGTGGAGGA TGTGCGGCCG CCGCACAAGG TGACAGACTA CTCCTCCTCC 
    AGCGAGGAGT CGGGGACAAC GGACGAGGAG GACGACGACG TGGAGCAGGA GGGGGCCGAG 
    GAGCCTACGT CTGGCCCGGA GGACACCAGA GCTGCATCAT CTCTGAACTT GAGCAATGGT 
    GAAACAGAGT CCGTGAAAAC CATGGTTGTC CATGACGACG TGGAGAGTGA ACCAGCCCTG 
    ACCCCGTCCA AAGAGGGCAC GCTGATCGTC CGCCAGAGTG CAGTTGACCA AAAGCGCGCC 
    AGCCATCATG AGAGCAATGG CTTTGCGGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG 
    CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCTTCCC CATCCTCCAG CCAGCCGACA 
    CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTA CTAATGAGAC TCAGTCCGCT 
    AGTAACACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
    TTACTACAGA TTTCTCCATC TAGCGGGACA ACAGTGACTT CTGTGGTGGG GTTTTCCTGT 
    GATGGAATGA GACCTGAGGC CATGAGGCAA GATCCTACGC GGAAGGGCTC AGTGGTCAAT 
    GTCAATCCCA CCAATACTCG GCCCCAGAGC GACACCCCAG AGATTCGCAA ATACAAGAAG 
    AGGTTTAACT CCGAGATCCT GTGCGCAGCC TTGTGGGGAG TGAACTTGTT GGTGGGTACC 
    GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTGTATCC TCTGATCAAC 
    AGAAGGCGAT TTCAGCAGAT GGACGTCCTG GAAGGCTTGA ACGTCTTAGT GACGATATCT 
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
    AACGACCCGG AGGTTGAGAA GAAGCAGGGC TGGACGACAG TGGGGGACCT GGAAGGCTGT 
    GTGCATTATA AAGTTGTAAA ATATGAAAGG ATCAAATTTC TGGTCATTGC TTTGAAGAAC 
    TCTGTGGAAG TCTACGCATG GGCACCCAAG CCATATCACA AATTCATGGC CTTTAAGTCA 
    TTTGGTGAGC TGGTGCACAA GCCGTTGCTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
    CTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA CTCAGGATCG 
    GTCTATGACA TTTACCTACC AACACACGTA AGAAAGAACC CACACTCTAT GATCCAATGT 
    AGCATCAAAC CCCACGCAAT CATCATCCTC CCCAACACAG ATGGCATGGA GCTCCTGGTG 
    TGCTATGAAG ATGAAGGGGT TTACGTGAAC ACGTACGGGA GGATCACCAA GGACGTGGTT 
    CTGCAGTGGG GAGAGATGCC AACGTCTGTA GCATATATTC GATCCAATCA GACGATGGGC 
    TGGGGAGAAA AGGCCATAGA GATCCGATCT GTGGAAACTG GCCACTTGGA TGGCGTGTTT 
    ATGCATAAAA GGGCTCAAAG GCTGAAGTTC TTGTGTGAAC GTAATGACAA GGTGTTCTTT 
    GCCTCCGTCC GGTCTGGGGG CAGCAGCCAG GTTTACTTCA TGACTCTCGG CAGGACCTCT 
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999338.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855106.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA.
    Price & Availability↑
    CloneID OBa220761
    Clone ID Related Accession (Same CDS sequence) XM_024999338.1
    Accession Version XM_024999338.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTCCCTG TGAGAACAAC ATCACGGTCC 
    CCTGTCCTGT CCCGCCGGGA TTCCCCACTG CAGGGTAGTG GGCAGCAGAG TAACCAGGCT 
    GGTCAGAGAA ACTCCACCAG CATCGAGCCG AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG 
    GCGCCCAGGC CGGGCAGTGG CAGCTCCTCG GGGTCCAGCA ACTCGGGGTC CCAGCCCGGC 
    TCCCACCCAG GGTCTCAGAG TGGCTCCGGG GAGCGCTTCA GAGTGAGATC ATCATCCAAA 
    TCTGAAGGCT CGCCATCTCA GCGCCTTGAA AACGCAGCAA AAAAGCCTGA AGATAAAAAG 
    GAAGTGTTTA GACCTCTCAA GCCTGCTGGT GAAGTGGACC TGACGGCTCT GGCCAAAGAG 
    CTGCGGGCCG TGGAGGATGT GCGGCCGCCG CACAAGGTGA CAGACTACTC CTCCTCCAGC 
    GAGGAGTCGG GGACAACGGA CGAGGAGGAC GACGACGTGG AGCAGGAGGG GGCCGAGGAG 
    CCTACGTCTG GCCCGGAGGA CACCAGAGCT GCATCATCTC TGAACTTGAG CAATGGTGAA 
    ACAGAGTCCG TGAAAACCAT GGTTGTCCAT GACGACGTGG AGAGTGAACC AGCCCTGACC 
    CCGTCCAAAG AGGGCACGCT GATCGTCCGC CAGAGTGCAG TTGACCAAAA GCGCGCCAGC 
    CATCATGAGA GCAATGGCTT TGCGGGTCGC ATTCACCTCT TGCCAGATCT CTTACAGCAA 
    AGCCATTCCT CCTCCACTTC CTCCACCTCC TCTTCCCCAT CCTCCAGCCA GCCGACACCC 
    ACCATGTCCC CACAGACACC CCAGGACAAG CTCACTACTA ATGAGACTCA GTCCGCTAGT 
    AACACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA 
    CTACAGATTT CTCCATCTAG CGGGACAACA GTGACTTCTG TGGTGGGGTT TTCCTGTGAT 
    GGAATGAGAC CTGAGGCCAT GAGGCAAGAT CCTACGCGGA AGGGCTCAGT GGTCAATGTC 
    AATCCCACCA ATACTCGGCC CCAGAGCGAC ACCCCAGAGA TTCGCAAATA CAAGAAGAGG 
    TTTAACTCCG AGATCCTGTG CGCAGCCTTG TGGGGAGTGA ACTTGTTGGT GGGTACCGAG 
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TGTATCCTCT GATCAACAGA 
    AGGCGATTTC AGCAGATGGA CGTCCTGGAA GGCTTGAACG TCTTAGTGAC GATATCTGGC 
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAC 
    GACCCGGAGG TTGAGAAGAA GCAGGGCTGG ACGACAGTGG GGGACCTGGA AGGCTGTGTG 
    CATTATAAAG TTGTAAAATA TGAAAGGATC AAATTTCTGG TCATTGCTTT GAAGAACTCT 
    GTGGAAGTCT ACGCATGGGC ACCCAAGCCA TATCACAAAT TCATGGCCTT TAAGTCATTT 
    GGTGAGCTGG TGCACAAGCC GTTGCTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGCTG 
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGACTC AGGATCGGTC 
    TATGACATTT ACCTACCAAC ACACGTAAGA AAGAACCCAC ACTCTATGAT CCAATGTAGC 
    ATCAAACCCC ACGCAATCAT CATCCTCCCC AACACAGATG GCATGGAGCT CCTGGTGTGC 
    TATGAAGATG AAGGGGTTTA CGTGAACACG TACGGGAGGA TCACCAAGGA CGTGGTTCTG 
    CAGTGGGGAG AGATGCCAAC GTCTGTAGCA TATATTCGAT CCAATCAGAC GATGGGCTGG 
    GGAGAAAAGG CCATAGAGAT CCGATCTGTG GAAACTGGCC ACTTGGATGG CGTGTTTATG 
    CATAAAAGGG CTCAAAGGCT GAAGTTCTTG TGTGAACGTA ATGACAAGGT GTTCTTTGCC 
    TCCGTCCGGT CTGGGGGCAG CAGCCAGGTT TACTTCATGA CTCTCGGCAG GACCTCTCTT 
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855106.1
    CDS121..4032
    Translation

    Target ORF information:

    RefSeq Version XM_024999338.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999338.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTCCCTG TGAGAACAAC ATCACGGTCC 
    CCTGTCCTGT CCCGCCGGGA TTCCCCACTG CAGGGTAGTG GGCAGCAGAG TAACCAGGCT 
    GGTCAGAGAA ACTCCACCAG CATCGAGCCG AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG 
    GCGCCCAGGC CGGGCAGTGG CAGCTCCTCG GGGTCCAGCA ACTCGGGGTC CCAGCCCGGC 
    TCCCACCCAG GGTCTCAGAG TGGCTCCGGG GAGCGCTTCA GAGTGAGATC ATCATCCAAA 
    TCTGAAGGCT CGCCATCTCA GCGCCTTGAA AACGCAGCAA AAAAGCCTGA AGATAAAAAG 
    GAAGTGTTTA GACCTCTCAA GCCTGCTGGT GAAGTGGACC TGACGGCTCT GGCCAAAGAG 
    CTGCGGGCCG TGGAGGATGT GCGGCCGCCG CACAAGGTGA CAGACTACTC CTCCTCCAGC 
    GAGGAGTCGG GGACAACGGA CGAGGAGGAC GACGACGTGG AGCAGGAGGG GGCCGAGGAG 
    CCTACGTCTG GCCCGGAGGA CACCAGAGCT GCATCATCTC TGAACTTGAG CAATGGTGAA 
    ACAGAGTCCG TGAAAACCAT GGTTGTCCAT GACGACGTGG AGAGTGAACC AGCCCTGACC 
    CCGTCCAAAG AGGGCACGCT GATCGTCCGC CAGAGTGCAG TTGACCAAAA GCGCGCCAGC 
    CATCATGAGA GCAATGGCTT TGCGGGTCGC ATTCACCTCT TGCCAGATCT CTTACAGCAA 
    AGCCATTCCT CCTCCACTTC CTCCACCTCC TCTTCCCCAT CCTCCAGCCA GCCGACACCC 
    ACCATGTCCC CACAGACACC CCAGGACAAG CTCACTACTA ATGAGACTCA GTCCGCTAGT 
    AACACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA 
    CTACAGATTT CTCCATCTAG CGGGACAACA GTGACTTCTG TGGTGGGGTT TTCCTGTGAT 
    GGAATGAGAC CTGAGGCCAT GAGGCAAGAT CCTACGCGGA AGGGCTCAGT GGTCAATGTC 
    AATCCCACCA ATACTCGGCC CCAGAGCGAC ACCCCAGAGA TTCGCAAATA CAAGAAGAGG 
    TTTAACTCCG AGATCCTGTG CGCAGCCTTG TGGGGAGTGA ACTTGTTGGT GGGTACCGAG 
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TGTATCCTCT GATCAACAGA 
    AGGCGATTTC AGCAGATGGA CGTCCTGGAA GGCTTGAACG TCTTAGTGAC GATATCTGGC 
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAC 
    GACCCGGAGG TTGAGAAGAA GCAGGGCTGG ACGACAGTGG GGGACCTGGA AGGCTGTGTG 
    CATTATAAAG TTGTAAAATA TGAAAGGATC AAATTTCTGG TCATTGCTTT GAAGAACTCT 
    GTGGAAGTCT ACGCATGGGC ACCCAAGCCA TATCACAAAT TCATGGCCTT TAAGTCATTT 
    GGTGAGCTGG TGCACAAGCC GTTGCTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGCTG 
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGACTC AGGATCGGTC 
    TATGACATTT ACCTACCAAC ACACGTAAGA AAGAACCCAC ACTCTATGAT CCAATGTAGC 
    ATCAAACCCC ACGCAATCAT CATCCTCCCC AACACAGATG GCATGGAGCT CCTGGTGTGC 
    TATGAAGATG AAGGGGTTTA CGTGAACACG TACGGGAGGA TCACCAAGGA CGTGGTTCTG 
    CAGTGGGGAG AGATGCCAAC GTCTGTAGCA TATATTCGAT CCAATCAGAC GATGGGCTGG 
    GGAGAAAAGG CCATAGAGAT CCGATCTGTG GAAACTGGCC ACTTGGATGG CGTGTTTATG 
    CATAAAAGGG CTCAAAGGCT GAAGTTCTTG TGTGAACGTA ATGACAAGGT GTTCTTTGCC 
    TCCGTCCGGT CTGGGGGCAG CAGCCAGGTT TACTTCATGA CTCTCGGCAG GACCTCTCTT 
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999339.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855107.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X18, mRNA.
    Price & Availability↑
    CloneID OBa220762
    Clone ID Related Accession (Same CDS sequence) XM_024999339.1
    Accession Version XM_024999339.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTCCCTG TGAGAACAAC ATCACGGTCC 
    CCTGTCCTGT CCCGCCGGGA TTCCCCACTG CAGGGTAGTG GGCAGCAGAG TAACCAGGCT 
    GGTCAGAGAA ACTCCACCAG TAGCATCGAG CCGAGGCTGC TCTGGGAGAG AGTGGAGAAG 
    CTGGCGCCCA GGCCGGGCAG TGGCAGCTCC TCGGGGTCCA GCAACTCGGG GTCCCAGCCC 
    GGCTCCCACC CAGGGTCTCA GAGTGGCTCC GGGGAGCGCT TCAGAGTGAG ATCATCATCC 
    AAATCTGAAG GCTCGCCATC TCAGCGCCTT GAAAACGCAG CAAAAAAGCC TGAAGATAAA 
    AAGGAAGTGT TTAGACCTCT CAAGCCTGCT GACCTGACGG CTCTGGCCAA AGAGCTGCGG 
    GCCGTGGAGG ATGTGCGGCC GCCGCACAAG GTGACAGACT ACTCCTCCTC CAGCGAGGAG 
    TCGGGGACAA CGGACGAGGA GGACGACGAC GTGGAGCAGG AGGGGGCCGA GGAGCCTACG 
    TCTGGCCCGG AGGACACCAG AGCTGCATCA TCTCTGAACT TGAGCAATGG TGAAACAGAG 
    TCCGTGAAAA CCATGGTTGT CCATGACGAC GTGGAGAGTG AACCAGCCCT GACCCCGTCC 
    AAAGAGGGCA CGCTGATCGT CCGCCAGAGT GCAGTTGACC AAAAGCGCGC CAGCCATCAT 
    GAGAGCAATG GCTTTGCGGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
    TCCTCCTCCA CTTCCTCCAC CTCCTCTTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
    TCCCCACAGA CACCCCAGGA CAAGCTCACT ACTAATGAGA CTCAGTCCGC TAGTAACACA 
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
    ATTTCTCCAT CTAGCGGGAC AACAGTGACT TCTGTGGTGG GGTTTTCCTG TGATGGAATG 
    AGACCTGAGG CCATGAGGCA AGATCCTACG CGGAAGGGCT CAGTGGTCAA TGTCAATCCC 
    ACCAATACTC GGCCCCAGAG CGACACCCCA GAGATTCGCA AATACAAGAA GAGGTTTAAC 
    TCCGAGATCC TGTGCGCAGC CTTGTGGGGA GTGAACTTGT TGGTGGGTAC CGAGAGTGGC 
    CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTGTATC CTCTGATCAA CAGAAGGCGA 
    TTTCAGCAGA TGGACGTCCT GGAAGGCTTG AACGTCTTAG TGACGATATC TGGCAAAAAG 
    GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAACGACCCG 
    GAGGTTGAGA AGAAGCAGGG CTGGACGACA GTGGGGGACC TGGAAGGCTG TGTGCATTAT 
    AAAGTTGTAA AATATGAAAG GATCAAATTT CTGGTCATTG CTTTGAAGAA CTCTGTGGAA 
    GTCTACGCAT GGGCACCCAA GCCATATCAC AAATTCATGG CCTTTAAGTC ATTTGGTGAG 
    CTGGTGCACA AGCCGTTGCT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GCTGAAAGTG 
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ACTCAGGATC GGTCTATGAC 
    ATTTACCTAC CAACACACGT AAGAAAGAAC CCACACTCTA TGATCCAATG TAGCATCAAA 
    CCCCACGCAA TCATCATCCT CCCCAACACA GATGGCATGG AGCTCCTGGT GTGCTATGAA 
    GATGAAGGGG TTTACGTGAA CACGTACGGG AGGATCACCA AGGACGTGGT TCTGCAGTGG 
    GGAGAGATGC CAACGTCTGT AGCATATATT CGATCCAATC AGACGATGGG CTGGGGAGAA 
    AAGGCCATAG AGATCCGATC TGTGGAAACT GGCCACTTGG ATGGCGTGTT TATGCATAAA 
    AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA CGTAATGACA AGGTGTTCTT TGCCTCCGTC 
    CGGTCTGGGG GCAGCAGCCA GGTTTACTTC ATGACTCTCG GCAGGACCTC TCTTCTGAGC 
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855107.1
    CDS121..4026
    Translation

    Target ORF information:

    RefSeq Version XM_024999339.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999339.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTCCCTG TGAGAACAAC ATCACGGTCC 
    CCTGTCCTGT CCCGCCGGGA TTCCCCACTG CAGGGTAGTG GGCAGCAGAG TAACCAGGCT 
    GGTCAGAGAA ACTCCACCAG TAGCATCGAG CCGAGGCTGC TCTGGGAGAG AGTGGAGAAG 
    CTGGCGCCCA GGCCGGGCAG TGGCAGCTCC TCGGGGTCCA GCAACTCGGG GTCCCAGCCC 
    GGCTCCCACC CAGGGTCTCA GAGTGGCTCC GGGGAGCGCT TCAGAGTGAG ATCATCATCC 
    AAATCTGAAG GCTCGCCATC TCAGCGCCTT GAAAACGCAG CAAAAAAGCC TGAAGATAAA 
    AAGGAAGTGT TTAGACCTCT CAAGCCTGCT GACCTGACGG CTCTGGCCAA AGAGCTGCGG 
    GCCGTGGAGG ATGTGCGGCC GCCGCACAAG GTGACAGACT ACTCCTCCTC CAGCGAGGAG 
    TCGGGGACAA CGGACGAGGA GGACGACGAC GTGGAGCAGG AGGGGGCCGA GGAGCCTACG 
    TCTGGCCCGG AGGACACCAG AGCTGCATCA TCTCTGAACT TGAGCAATGG TGAAACAGAG 
    TCCGTGAAAA CCATGGTTGT CCATGACGAC GTGGAGAGTG AACCAGCCCT GACCCCGTCC 
    AAAGAGGGCA CGCTGATCGT CCGCCAGAGT GCAGTTGACC AAAAGCGCGC CAGCCATCAT 
    GAGAGCAATG GCTTTGCGGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
    TCCTCCTCCA CTTCCTCCAC CTCCTCTTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
    TCCCCACAGA CACCCCAGGA CAAGCTCACT ACTAATGAGA CTCAGTCCGC TAGTAACACA 
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
    ATTTCTCCAT CTAGCGGGAC AACAGTGACT TCTGTGGTGG GGTTTTCCTG TGATGGAATG 
    AGACCTGAGG CCATGAGGCA AGATCCTACG CGGAAGGGCT CAGTGGTCAA TGTCAATCCC 
    ACCAATACTC GGCCCCAGAG CGACACCCCA GAGATTCGCA AATACAAGAA GAGGTTTAAC 
    TCCGAGATCC TGTGCGCAGC CTTGTGGGGA GTGAACTTGT TGGTGGGTAC CGAGAGTGGC 
    CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTGTATC CTCTGATCAA CAGAAGGCGA 
    TTTCAGCAGA TGGACGTCCT GGAAGGCTTG AACGTCTTAG TGACGATATC TGGCAAAAAG 
    GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAACGACCCG 
    GAGGTTGAGA AGAAGCAGGG CTGGACGACA GTGGGGGACC TGGAAGGCTG TGTGCATTAT 
    AAAGTTGTAA AATATGAAAG GATCAAATTT CTGGTCATTG CTTTGAAGAA CTCTGTGGAA 
    GTCTACGCAT GGGCACCCAA GCCATATCAC AAATTCATGG CCTTTAAGTC ATTTGGTGAG 
    CTGGTGCACA AGCCGTTGCT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GCTGAAAGTG 
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ACTCAGGATC GGTCTATGAC 
    ATTTACCTAC CAACACACGT AAGAAAGAAC CCACACTCTA TGATCCAATG TAGCATCAAA 
    CCCCACGCAA TCATCATCCT CCCCAACACA GATGGCATGG AGCTCCTGGT GTGCTATGAA 
    GATGAAGGGG TTTACGTGAA CACGTACGGG AGGATCACCA AGGACGTGGT TCTGCAGTGG 
    GGAGAGATGC CAACGTCTGT AGCATATATT CGATCCAATC AGACGATGGG CTGGGGAGAA 
    AAGGCCATAG AGATCCGATC TGTGGAAACT GGCCACTTGG ATGGCGTGTT TATGCATAAA 
    AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA CGTAATGACA AGGTGTTCTT TGCCTCCGTC 
    CGGTCTGGGG GCAGCAGCCA GGTTTACTTC ATGACTCTCG GCAGGACCTC TCTTCTGAGC 
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999340.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855108.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X19, mRNA.
    Price & Availability↑
    CloneID OBa220763
    Clone ID Related Accession (Same CDS sequence) XM_024999340.1
    Accession Version XM_024999340.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT ACAGTGGTCC CACCTGGCAT CTCTCAAGAA CAATGTTTCC 
    CCTGTCTCGA GATCCCATTC CTTCAGTGAC CCTTCTCCTC CCAAATTTGC ACACCACCAT 
    CTTCGCTCTC AGGACCCCTG CCCACCTTCC CGCAGTGAGG TGCTCAGTCA GAGTTCTGAC 
    TCTAAGGCGG AGGTGCCCGA CCCAGCCCAG AAGGCTTGGT CTAGATCAGA CAGTGACGAG 
    GTGCCTCCAC GGGTCCCTGT GAGAACAACA TCACGGTCCC CTGTCCTGTC CCGCCGGGAT 
    TCCCCACTGC AGGGTAGTGG GCAGCAGAGT AACCAGGCTG GTCAGAGAAA CTCCACCAGT 
    AGCATCGAGC CGAGGCTGCT CTGGGAGAGA GTGGAGAAGC TGGCGCCCAG GCCGGGCAGT 
    GGCAGCTCCT CGGGGTCCAG CAACTCGGGG TCCCAGCCCG GCTCCCACCC AGGGTCTCAG 
    AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA TCATCATCCA AATCTGAAGG CTCGCCATCT 
    CAGCGCCTTG AAAACGCAGC AAAAAAGCCT GAAGATAAAA AGGAAGTGTT TAGACCTCTC 
    AAGCCTGCTG GTGAAGTGGA CCTGACGGCT CTGGCCAAAG AGCTGCGGGC CGTGGAGGAT 
    GTGCGGCCGC CGCACAAGGT GACAGACTAC TCCTCCTCCA GCGAGGAGTC GGGGACAACG 
    GACGAGGAGG ACGACGACGT GGAGCAGGAG GGGGCCGAGG AGCCTACGTC TGGCCCGGAG 
    GACACCAGAG CTGCATCATC TCTGAACTTG AGCAATGGTG AAACAGAGTC CGTGAAAACC 
    ATGGTTGTCC ATGACGACGT GGAGAGTGAA CCAGCCCTGA CCCCGTCCAA AGAGGGCACG 
    CTGATCGTCC GCCAGAGTGC AGTTGACCAA AAGCGCGCCA GCCATCATGA GAGCAATGGC 
    TTTGCGGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT 
    TCCTCCACCT CCTCTTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA 
    CCCCAGGACA AGCTCACTAC TAATGAGACT CAGTCCGCTA GTAACACACT CCAGAAACAC 
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
    AGCGGGACAA CAGTGACTTC TGTGGTGGGG TTTTCCTGTG ATGGAATGAG ACCTGAGGCC 
    ATGAGGCAAG ATCCTACGCG GAAGGGCTCA GTGGTCAATG TCAATCCCAC CAATACTCGG 
    CCCCAGAGCG ACACCCCAGA GATTCGCAAA TACAAGAAGA GGTTTAACTC CGAGATCCTG 
    TGCGCAGCCT TGTGGGGAGT GAACTTGTTG GTGGGTACCG AGAGTGGCCT GATGCTGCTG 
    GACAGAAGTG GCCAAGGGAA GGTGTATCCT CTGATCAACA GAAGGCGATT TCAGCAGATG 
    GACGTCCTGG AAGGCTTGAA CGTCTTAGTG ACGATATCTG GCAAAAAGGA TAAGTTACGT 
    GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ACGACCCGGA GGTTGAGAAG 
    AAGCAGGGCT GGACGACAGT GGGGGACCTG GAAGGCTGTG TGCATTATAA AGTTGTAAAA 
    TATGAAAGGA TCAAATTTCT GGTCATTGCT TTGAAGAACT CTGTGGAAGT CTACGCATGG 
    GCACCCAAGC CATATCACAA ATTCATGGCC TTTAAGTCAT TTGGTGAGCT GGTGCACAAG 
    CCGTTGCTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGC TGAAAGTGAT CTATGGATCC 
    TGTGCTGGAT TCCATGCTGT TGATGTGGAC TCAGGATCGG TCTATGACAT TTACCTACCA 
    ACACACGTAA GAAAGAACCC ACACTCTATG ATCCAATGTA GCATCAAACC CCACGCAATC 
    ATCATCCTCC CCAACACAGA TGGCATGGAG CTCCTGGTGT GCTATGAAGA TGAAGGGGTT 
    TACGTGAACA CGTACGGGAG GATCACCAAG GACGTGGTTC TGCAGTGGGG AGAGATGCCA 
    ACGTCTGTAG CATATATTCG ATCCAATCAG ACGATGGGCT GGGGAGAAAA GGCCATAGAG 
    ATCCGATCTG TGGAAACTGG CCACTTGGAT GGCGTGTTTA TGCATAAAAG GGCTCAAAGG 
    CTGAAGTTCT TGTGTGAACG TAATGACAAG GTGTTCTTTG CCTCCGTCCG GTCTGGGGGC 
    AGCAGCCAGG TTTACTTCAT GACTCTCGGC AGGACCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855108.1
    CDS122..4015
    Translation

    Target ORF information:

    RefSeq Version XM_024999340.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999340.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT ACAGTGGTCC CACCTGGCAT CTCTCAAGAA CAATGTTTCC 
    CCTGTCTCGA GATCCCATTC CTTCAGTGAC CCTTCTCCTC CCAAATTTGC ACACCACCAT 
    CTTCGCTCTC AGGACCCCTG CCCACCTTCC CGCAGTGAGG TGCTCAGTCA GAGTTCTGAC 
    TCTAAGGCGG AGGTGCCCGA CCCAGCCCAG AAGGCTTGGT CTAGATCAGA CAGTGACGAG 
    GTGCCTCCAC GGGTCCCTGT GAGAACAACA TCACGGTCCC CTGTCCTGTC CCGCCGGGAT 
    TCCCCACTGC AGGGTAGTGG GCAGCAGAGT AACCAGGCTG GTCAGAGAAA CTCCACCAGT 
    AGCATCGAGC CGAGGCTGCT CTGGGAGAGA GTGGAGAAGC TGGCGCCCAG GCCGGGCAGT 
    GGCAGCTCCT CGGGGTCCAG CAACTCGGGG TCCCAGCCCG GCTCCCACCC AGGGTCTCAG 
    AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA TCATCATCCA AATCTGAAGG CTCGCCATCT 
    CAGCGCCTTG AAAACGCAGC AAAAAAGCCT GAAGATAAAA AGGAAGTGTT TAGACCTCTC 
    AAGCCTGCTG GTGAAGTGGA CCTGACGGCT CTGGCCAAAG AGCTGCGGGC CGTGGAGGAT 
    GTGCGGCCGC CGCACAAGGT GACAGACTAC TCCTCCTCCA GCGAGGAGTC GGGGACAACG 
    GACGAGGAGG ACGACGACGT GGAGCAGGAG GGGGCCGAGG AGCCTACGTC TGGCCCGGAG 
    GACACCAGAG CTGCATCATC TCTGAACTTG AGCAATGGTG AAACAGAGTC CGTGAAAACC 
    ATGGTTGTCC ATGACGACGT GGAGAGTGAA CCAGCCCTGA CCCCGTCCAA AGAGGGCACG 
    CTGATCGTCC GCCAGAGTGC AGTTGACCAA AAGCGCGCCA GCCATCATGA GAGCAATGGC 
    TTTGCGGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT 
    TCCTCCACCT CCTCTTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA 
    CCCCAGGACA AGCTCACTAC TAATGAGACT CAGTCCGCTA GTAACACACT CCAGAAACAC 
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
    AGCGGGACAA CAGTGACTTC TGTGGTGGGG TTTTCCTGTG ATGGAATGAG ACCTGAGGCC 
    ATGAGGCAAG ATCCTACGCG GAAGGGCTCA GTGGTCAATG TCAATCCCAC CAATACTCGG 
    CCCCAGAGCG ACACCCCAGA GATTCGCAAA TACAAGAAGA GGTTTAACTC CGAGATCCTG 
    TGCGCAGCCT TGTGGGGAGT GAACTTGTTG GTGGGTACCG AGAGTGGCCT GATGCTGCTG 
    GACAGAAGTG GCCAAGGGAA GGTGTATCCT CTGATCAACA GAAGGCGATT TCAGCAGATG 
    GACGTCCTGG AAGGCTTGAA CGTCTTAGTG ACGATATCTG GCAAAAAGGA TAAGTTACGT 
    GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ACGACCCGGA GGTTGAGAAG 
    AAGCAGGGCT GGACGACAGT GGGGGACCTG GAAGGCTGTG TGCATTATAA AGTTGTAAAA 
    TATGAAAGGA TCAAATTTCT GGTCATTGCT TTGAAGAACT CTGTGGAAGT CTACGCATGG 
    GCACCCAAGC CATATCACAA ATTCATGGCC TTTAAGTCAT TTGGTGAGCT GGTGCACAAG 
    CCGTTGCTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGC TGAAAGTGAT CTATGGATCC 
    TGTGCTGGAT TCCATGCTGT TGATGTGGAC TCAGGATCGG TCTATGACAT TTACCTACCA 
    ACACACGTAA GAAAGAACCC ACACTCTATG ATCCAATGTA GCATCAAACC CCACGCAATC 
    ATCATCCTCC CCAACACAGA TGGCATGGAG CTCCTGGTGT GCTATGAAGA TGAAGGGGTT 
    TACGTGAACA CGTACGGGAG GATCACCAAG GACGTGGTTC TGCAGTGGGG AGAGATGCCA 
    ACGTCTGTAG CATATATTCG ATCCAATCAG ACGATGGGCT GGGGAGAAAA GGCCATAGAG 
    ATCCGATCTG TGGAAACTGG CCACTTGGAT GGCGTGTTTA TGCATAAAAG GGCTCAAAGG 
    CTGAAGTTCT TGTGTGAACG TAATGACAAG GTGTTCTTTG CCTCCGTCCG GTCTGGGGGC 
    AGCAGCCAGG TTTACTTCAT GACTCTCGGC AGGACCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999341.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855109.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X20, mRNA.
    Price & Availability↑
    CloneID OBa220764
    Clone ID Related Accession (Same CDS sequence) XM_024999341.1
    Accession Version XM_024999341.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC 
    TCGAGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC 
    TCTCAGGACC CCTGCCCACC TTCCCGCAGT GAGGTGCTCA GTCAGAGTTC TGACTCTAAG 
    GCGGAGGTGC CCGACCCAGC CCAGAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT 
    CCACGGGTCC CTGTGAGAAC AACATCACGG TCCCCTGTCC TGTCCCGCCG GGATTCCCCA 
    CTGCAGGGTA GTGGGCAGCA GAGTAACCAG GCTGGTCAGA GAAACTCCAC CAGTAGCATC 
    GAGCCGAGGC TGCTCTGGGA GAGAGTGGAG AAGCTGGCGC CCAGGCCGGG CAGTGGCAGC 
    TCCTCGGGGT CCAGCAACTC GGGGTCCCAG CCCGGCTCCC ACCCAGGGTC TCAGAGTGGC 
    TCCGGGGAGC GCTTCAGAGT GAGATCATCA TCCAAATCTG AAGGCTCGCC ATCTCAGCGC 
    CTTGAAAACG CAGCAAAAAA GCCTGAAGAT AAAAAGGAAG TGTTTAGACC TCTCAAGCCT 
    GCTGGTGAAG TGGACCTGAC GGCTCTGGCC AAAGAGCTGC GGGCCGTGGA GGATGTGCGG 
    CCGCCGCACA AGGTGACAGA CTACTCCTCC TCCAGCGAGG AGTCGGGGAC AACGGACGAG 
    GAGGACGACG ACGTGGAGCA GGAGGGGGCC GAGGAGCCTA CGTCTGGCCC GGAGGACACC 
    AGAGCTGCAT CATCTCTGAA CTTGAGCAAT GGTGAAACAG AGTCCGTGAA AACCATGGTT 
    GTCCATGACG ACGTGGAGAG TGAACCAGCC CTGACCCCGT CCAAAGAGGG CACGCTGATC 
    GTCCGCCAGA GTGCAGTTGA CCAAAAGCGC GCCAGCCATC ATGAGAGCAA TGGCTTTGCG 
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC 
    ACCTCCTCTT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG 
    GACAAGCTCA CTACTAATGA GACTCAGTCC GCTAGTAACA CACTCCAGAA ACACAAATCT 
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGCGGG 
    ACAACAGTGA CTTCTGTGGT GGGGTTTTCC TGTGATGGAA TGAGACCTGA GGCCATGAGG 
    CAAGATCCTA CGCGGAAGGG CTCAGTGGTC AATGTCAATC CCACCAATAC TCGGCCCCAG 
    AGCGACACCC CAGAGATTCG CAAATACAAG AAGAGGTTTA ACTCCGAGAT CCTGTGCGCA 
    GCCTTGTGGG GAGTGAACTT GTTGGTGGGT ACCGAGAGTG GCCTGATGCT GCTGGACAGA 
    AGTGGCCAAG GGAAGGTGTA TCCTCTGATC AACAGAAGGC GATTTCAGCA GATGGACGTC 
    CTGGAAGGCT TGAACGTCTT AGTGACGATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC 
    TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAACGACC CGGAGGTTGA GAAGAAGCAG 
    GGCTGGACGA CAGTGGGGGA CCTGGAAGGC TGTGTGCATT ATAAAGTTGT AAAATATGAA 
    AGGATCAAAT TTCTGGTCAT TGCTTTGAAG AACTCTGTGG AAGTCTACGC ATGGGCACCC 
    AAGCCATATC ACAAATTCAT GGCCTTTAAG TCATTTGGTG AGCTGGTGCA CAAGCCGTTG 
    CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGCTGAAAG TGATCTATGG ATCCTGTGCT 
    GGATTCCATG CTGTTGATGT GGACTCAGGA TCGGTCTATG ACATTTACCT ACCAACACAC 
    GTAAGAAAGA ACCCACACTC TATGATCCAA TGTAGCATCA AACCCCACGC AATCATCATC 
    CTCCCCAACA CAGATGGCAT GGAGCTCCTG GTGTGCTATG AAGATGAAGG GGTTTACGTG 
    AACACGTACG GGAGGATCAC CAAGGACGTG GTTCTGCAGT GGGGAGAGAT GCCAACGTCT 
    GTAGCATATA TTCGATCCAA TCAGACGATG GGCTGGGGAG AAAAGGCCAT AGAGATCCGA 
    TCTGTGGAAA CTGGCCACTT GGATGGCGTG TTTATGCATA AAAGGGCTCA AAGGCTGAAG 
    TTCTTGTGTG AACGTAATGA CAAGGTGTTC TTTGCCTCCG TCCGGTCTGG GGGCAGCAGC 
    CAGGTTTACT TCATGACTCT CGGCAGGACC TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855109.1
    CDS122..4009
    Translation

    Target ORF information:

    RefSeq Version XM_024999341.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999341.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC 
    TCGAGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC 
    TCTCAGGACC CCTGCCCACC TTCCCGCAGT GAGGTGCTCA GTCAGAGTTC TGACTCTAAG 
    GCGGAGGTGC CCGACCCAGC CCAGAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT 
    CCACGGGTCC CTGTGAGAAC AACATCACGG TCCCCTGTCC TGTCCCGCCG GGATTCCCCA 
    CTGCAGGGTA GTGGGCAGCA GAGTAACCAG GCTGGTCAGA GAAACTCCAC CAGTAGCATC 
    GAGCCGAGGC TGCTCTGGGA GAGAGTGGAG AAGCTGGCGC CCAGGCCGGG CAGTGGCAGC 
    TCCTCGGGGT CCAGCAACTC GGGGTCCCAG CCCGGCTCCC ACCCAGGGTC TCAGAGTGGC 
    TCCGGGGAGC GCTTCAGAGT GAGATCATCA TCCAAATCTG AAGGCTCGCC ATCTCAGCGC 
    CTTGAAAACG CAGCAAAAAA GCCTGAAGAT AAAAAGGAAG TGTTTAGACC TCTCAAGCCT 
    GCTGGTGAAG TGGACCTGAC GGCTCTGGCC AAAGAGCTGC GGGCCGTGGA GGATGTGCGG 
    CCGCCGCACA AGGTGACAGA CTACTCCTCC TCCAGCGAGG AGTCGGGGAC AACGGACGAG 
    GAGGACGACG ACGTGGAGCA GGAGGGGGCC GAGGAGCCTA CGTCTGGCCC GGAGGACACC 
    AGAGCTGCAT CATCTCTGAA CTTGAGCAAT GGTGAAACAG AGTCCGTGAA AACCATGGTT 
    GTCCATGACG ACGTGGAGAG TGAACCAGCC CTGACCCCGT CCAAAGAGGG CACGCTGATC 
    GTCCGCCAGA GTGCAGTTGA CCAAAAGCGC GCCAGCCATC ATGAGAGCAA TGGCTTTGCG 
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC 
    ACCTCCTCTT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG 
    GACAAGCTCA CTACTAATGA GACTCAGTCC GCTAGTAACA CACTCCAGAA ACACAAATCT 
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGCGGG 
    ACAACAGTGA CTTCTGTGGT GGGGTTTTCC TGTGATGGAA TGAGACCTGA GGCCATGAGG 
    CAAGATCCTA CGCGGAAGGG CTCAGTGGTC AATGTCAATC CCACCAATAC TCGGCCCCAG 
    AGCGACACCC CAGAGATTCG CAAATACAAG AAGAGGTTTA ACTCCGAGAT CCTGTGCGCA 
    GCCTTGTGGG GAGTGAACTT GTTGGTGGGT ACCGAGAGTG GCCTGATGCT GCTGGACAGA 
    AGTGGCCAAG GGAAGGTGTA TCCTCTGATC AACAGAAGGC GATTTCAGCA GATGGACGTC 
    CTGGAAGGCT TGAACGTCTT AGTGACGATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC 
    TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAACGACC CGGAGGTTGA GAAGAAGCAG 
    GGCTGGACGA CAGTGGGGGA CCTGGAAGGC TGTGTGCATT ATAAAGTTGT AAAATATGAA 
    AGGATCAAAT TTCTGGTCAT TGCTTTGAAG AACTCTGTGG AAGTCTACGC ATGGGCACCC 
    AAGCCATATC ACAAATTCAT GGCCTTTAAG TCATTTGGTG AGCTGGTGCA CAAGCCGTTG 
    CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGCTGAAAG TGATCTATGG ATCCTGTGCT 
    GGATTCCATG CTGTTGATGT GGACTCAGGA TCGGTCTATG ACATTTACCT ACCAACACAC 
    GTAAGAAAGA ACCCACACTC TATGATCCAA TGTAGCATCA AACCCCACGC AATCATCATC 
    CTCCCCAACA CAGATGGCAT GGAGCTCCTG GTGTGCTATG AAGATGAAGG GGTTTACGTG 
    AACACGTACG GGAGGATCAC CAAGGACGTG GTTCTGCAGT GGGGAGAGAT GCCAACGTCT 
    GTAGCATATA TTCGATCCAA TCAGACGATG GGCTGGGGAG AAAAGGCCAT AGAGATCCGA 
    TCTGTGGAAA CTGGCCACTT GGATGGCGTG TTTATGCATA AAAGGGCTCA AAGGCTGAAG 
    TTCTTGTGTG AACGTAATGA CAAGGTGTTC TTTGCCTCCG TCCGGTCTGG GGGCAGCAGC 
    CAGGTTTACT TCATGACTCT CGGCAGGACC TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999342.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855110.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X21, mRNA.
    Price & Availability↑
    CloneID OBa220765
    Clone ID Related Accession (Same CDS sequence) XM_024999342.1
    Accession Version XM_024999342.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTGTTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCGAGTG GCAACTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTTT CCCCTGTCTC GAGATCCCAT 
    TCCTTCAGTG ACCCTTCTCC TCCCAAATTT GCACACCACC ATCTTCGCTC TCAGGACCCC 
    TGCCCACCTT CCCGCAGTGA GGTGCTCAGT CAGAGTTCTG ACTCTAAGGC GGAGGTGCCC 
    GACCCAGCCC AGAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC ACGGGTCCCT 
    GTGAGAACAA CATCACGGTC CCCTGTCCTG TCCCGCCGGG ATTCCCCACT GCAGGGTAGT 
    GGGCAGCAGA GTAACCAGGC TGGTCAGAGA AACTCCACCA GTAGCATCGA GCCGAGGCTG 
    CTCTGGGAGA GAGTGGAGAA GCTGGCGCCC AGGCCGGGCA GTGGCAGCTC CTCGGGGTCC 
    AGCAACTCGG GGTCCCAGCC CGGCTCCCAC CCAGGGTCTC AGAGTGGCTC CGGGGAGCGC 
    TTCAGAGTGA GATCATCATC CAAATCTGAA GGCTCGCCAT CTCAGCGCCT TGAAAACGCA 
    GCAAAAAAGC CTGAAGATAA AAAGGAAGTG TTTAGACCTC TCAAGCCTGC TGGTGAAGTG 
    GACCTGACGG CTCTGGCCAA AGAGCTGCGG GCCGTGGAGG ATGTGCGGCC GCCGCACAAG 
    GTGACAGACT ACTCCTCCTC CAGCGAGGAG TCGGGGACAA CGGACGAGGA GGACGACGAC 
    GTGGAGCAGG AGGGGGCCGA GGAGCCTACG TCTGGCCCGG AGGACACCAG AGCTGCATCA 
    TCTCTGAACT TGAGCAATGG TGAAACAGAG TCCGTGAAAA CCATGGTTGT CCATGACGAC 
    GTGGAGAGTG AACCAGCCCT GACCCCGTCC AAAGAGGGCA CGCTGATCGT CCGCCAGACT 
    CAGTCCGCTA GTAACACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGGACAA CAGTGACTTC TGTGGTGGGG 
    TTTTCCTGTG ATGGAATGAG ACCTGAGGCC ATGAGGCAAG ATCCTACGCG GAAGGGCTCA 
    GTGGTCAATG TCAATCCCAC CAATACTCGG CCCCAGAGCG ACACCCCAGA GATTCGCAAA 
    TACAAGAAGA GGTTTAACTC CGAGATCCTG TGCGCAGCCT TGTGGGGAGT GAACTTGTTG 
    GTGGGTACCG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTGTATCCT 
    CTGATCAACA GAAGGCGATT TCAGCAGATG GACGTCCTGG AAGGCTTGAA CGTCTTAGTG 
    ACGATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
    ATACTTCACA ACGACCCGGA GGTTGAGAAG AAGCAGGGCT GGACGACAGT GGGGGACCTG 
    GAAGGCTGTG TGCATTATAA AGTTGTAAAA TATGAAAGGA TCAAATTTCT GGTCATTGCT 
    TTGAAGAACT CTGTGGAAGT CTACGCATGG GCACCCAAGC CATATCACAA ATTCATGGCC 
    TTTAAGTCAT TTGGTGAGCT GGTGCACAAG CCGTTGCTGG TGGATCTCAC TGTTGAGGAA 
    GGCCAGAGGC TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAC 
    TCAGGATCGG TCTATGACAT TTACCTACCA ACACACATCC AATGTAGCAT CAAACCCCAC 
    GCAATCATCA TCCTCCCCAA CACAGATGGC ATGGAGCTCC TGGTGTGCTA TGAAGATGAA 
    GGGGTTTACG TGAACACGTA CGGGAGGATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG 
    ATGCCAACGT CTGTAGCATA TATTCGATCC AATCAGACGA TGGGCTGGGG AGAAAAGGCC 
    ATAGAGATCC GATCTGTGGA AACTGGCCAC TTGGATGGCG TGTTTATGCA TAAAAGGGCT 
    CAAAGGCTGA AGTTCTTGTG TGAACGTAAT GACAAGGTGT TCTTTGCCTC CGTCCGGTCT 
    GGGGGCAGCA GCCAGGTTTA CTTCATGACT CTCGGCAGGA CCTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855110.1
    CDS122..3961
    Translation

    Target ORF information:

    RefSeq Version XM_024999342.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999342.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTGTTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCGAGTG GCAACTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTTT CCCCTGTCTC GAGATCCCAT 
    TCCTTCAGTG ACCCTTCTCC TCCCAAATTT GCACACCACC ATCTTCGCTC TCAGGACCCC 
    TGCCCACCTT CCCGCAGTGA GGTGCTCAGT CAGAGTTCTG ACTCTAAGGC GGAGGTGCCC 
    GACCCAGCCC AGAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC ACGGGTCCCT 
    GTGAGAACAA CATCACGGTC CCCTGTCCTG TCCCGCCGGG ATTCCCCACT GCAGGGTAGT 
    GGGCAGCAGA GTAACCAGGC TGGTCAGAGA AACTCCACCA GTAGCATCGA GCCGAGGCTG 
    CTCTGGGAGA GAGTGGAGAA GCTGGCGCCC AGGCCGGGCA GTGGCAGCTC CTCGGGGTCC 
    AGCAACTCGG GGTCCCAGCC CGGCTCCCAC CCAGGGTCTC AGAGTGGCTC CGGGGAGCGC 
    TTCAGAGTGA GATCATCATC CAAATCTGAA GGCTCGCCAT CTCAGCGCCT TGAAAACGCA 
    GCAAAAAAGC CTGAAGATAA AAAGGAAGTG TTTAGACCTC TCAAGCCTGC TGGTGAAGTG 
    GACCTGACGG CTCTGGCCAA AGAGCTGCGG GCCGTGGAGG ATGTGCGGCC GCCGCACAAG 
    GTGACAGACT ACTCCTCCTC CAGCGAGGAG TCGGGGACAA CGGACGAGGA GGACGACGAC 
    GTGGAGCAGG AGGGGGCCGA GGAGCCTACG TCTGGCCCGG AGGACACCAG AGCTGCATCA 
    TCTCTGAACT TGAGCAATGG TGAAACAGAG TCCGTGAAAA CCATGGTTGT CCATGACGAC 
    GTGGAGAGTG AACCAGCCCT GACCCCGTCC AAAGAGGGCA CGCTGATCGT CCGCCAGACT 
    CAGTCCGCTA GTAACACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGGACAA CAGTGACTTC TGTGGTGGGG 
    TTTTCCTGTG ATGGAATGAG ACCTGAGGCC ATGAGGCAAG ATCCTACGCG GAAGGGCTCA 
    GTGGTCAATG TCAATCCCAC CAATACTCGG CCCCAGAGCG ACACCCCAGA GATTCGCAAA 
    TACAAGAAGA GGTTTAACTC CGAGATCCTG TGCGCAGCCT TGTGGGGAGT GAACTTGTTG 
    GTGGGTACCG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTGTATCCT 
    CTGATCAACA GAAGGCGATT TCAGCAGATG GACGTCCTGG AAGGCTTGAA CGTCTTAGTG 
    ACGATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
    ATACTTCACA ACGACCCGGA GGTTGAGAAG AAGCAGGGCT GGACGACAGT GGGGGACCTG 
    GAAGGCTGTG TGCATTATAA AGTTGTAAAA TATGAAAGGA TCAAATTTCT GGTCATTGCT 
    TTGAAGAACT CTGTGGAAGT CTACGCATGG GCACCCAAGC CATATCACAA ATTCATGGCC 
    TTTAAGTCAT TTGGTGAGCT GGTGCACAAG CCGTTGCTGG TGGATCTCAC TGTTGAGGAA 
    GGCCAGAGGC TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAC 
    TCAGGATCGG TCTATGACAT TTACCTACCA ACACACATCC AATGTAGCAT CAAACCCCAC 
    GCAATCATCA TCCTCCCCAA CACAGATGGC ATGGAGCTCC TGGTGTGCTA TGAAGATGAA 
    GGGGTTTACG TGAACACGTA CGGGAGGATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG 
    ATGCCAACGT CTGTAGCATA TATTCGATCC AATCAGACGA TGGGCTGGGG AGAAAAGGCC 
    ATAGAGATCC GATCTGTGGA AACTGGCCAC TTGGATGGCG TGTTTATGCA TAAAAGGGCT 
    CAAAGGCTGA AGTTCTTGTG TGAACGTAAT GACAAGGTGT TCTTTGCCTC CGTCCGGTCT 
    GGGGGCAGCA GCCAGGTTTA CTTCATGACT CTCGGCAGGA CCTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999343.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855111.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X22, mRNA.
    Price & Availability↑
    CloneID OBa220766
    Clone ID Related Accession (Same CDS sequence) XM_024999343.1
    Accession Version XM_024999343.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTGTTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCGAGTG GCAACTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTCCCTGTGA GAACAACATC ACGGTCCCCT GTCCTGTCCC GCCGGGATTC CCCACTGCAG 
    GGTAGTGGGC AGCAGAGTAA CCAGGCTGGT CAGAGAAACT CCACCAGTAG CATCGAGCCG 
    AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG GCGCCCAGGC CGGGCAGTGG CAGCTCCTCG 
    GGGTCCAGCA ACTCGGGGTC CCAGCCCGGC TCCCACCCAG GGTCTCAGAG TGGCTCCGGG 
    GAGCGCTTCA GAGTGAGATC ATCATCCAAA TCTGAAGGCT CGCCATCTCA GCGCCTTGAA 
    AACGCAGCAA AAAAGCCTGA AGATAAAAAG GAAGTGTTTA GACCTCTCAA GCCTGCTGAC 
    CTGACGGCTC TGGCCAAAGA GCTGCGGGCC GTGGAGGATG TGCGGCCGCC GCACAAGGTG 
    ACAGACTACT CCTCCTCCAG CGAGGAGTCG GGGACAACGG ACGAGGAGGA CGACGACGTG 
    GAGCAGGAGG GGGCCGAGGA GCCTACGTCT GGCCCGGAGG ACACCAGAGC TGCATCATCT 
    CTGAACTTGA GCAATGGTGA AACAGAGTCC GTGAAAACCA TGGTTGTCCA TGACGACGTG 
    GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA GAGGGCACGC TGATCGTCCG CCAGAGTGCA 
    GTTGACCAAA AGCGCGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC 
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCTTCCCCA 
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTACT 
    AATGAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GCGGGACAAC AGTGACTTCT 
    GTGGTGGGGT TTTCCTGTGA TGGAATGAGA CCTGAGGCCA TGAGGCAAGA TCCTACGCGG 
    AAGGGCTCAG TGGTCAATGT CAATCCCACC AATACTCGGC CCCAGAGCGA CACCCCAGAG 
    ATTCGCAAAT ACAAGAAGAG GTTTAACTCC GAGATCCTGT GCGCAGCCTT GTGGGGAGTG 
    AACTTGTTGG TGGGTACCGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
    GTGTATCCTC TGATCAACAG AAGGCGATTT CAGCAGATGG ACGTCCTGGA AGGCTTGAAC 
    GTCTTAGTGA CGATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
    AGAAATAAAA TACTTCACAA CGACCCGGAG GTTGAGAAGA AGCAGGGCTG GACGACAGTG 
    GGGGACCTGG AAGGCTGTGT GCATTATAAA GTTGTAAAAT ATGAAAGGAT CAAATTTCTG 
    GTCATTGCTT TGAAGAACTC TGTGGAAGTC TACGCATGGG CACCCAAGCC ATATCACAAA 
    TTCATGGCCT TTAAGTCATT TGGTGAGCTG GTGCACAAGC CGTTGCTGGT GGATCTCACT 
    GTTGAGGAAG GCCAGAGGCT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
    GATGTGGACT CAGGATCGGT CTATGACATT TACCTACCAA CACACATCCA ATGTAGCATC 
    AAACCCCACG CAATCATCAT CCTCCCCAAC ACAGATGGCA TGGAGCTCCT GGTGTGCTAT 
    GAAGATGAAG GGGTTTACGT GAACACGTAC GGGAGGATCA CCAAGGACGT GGTTCTGCAG 
    TGGGGAGAGA TGCCAACGTC TGTAGCATAT ATTCGATCCA ATCAGACGAT GGGCTGGGGA 
    GAAAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGCCACT TGGATGGCGT GTTTATGCAT 
    AAAAGGGCTC AAAGGCTGAA GTTCTTGTGT GAACGTAATG ACAAGGTGTT CTTTGCCTCC 
    GTCCGGTCTG GGGGCAGCAG CCAGGTTTAC TTCATGACTC TCGGCAGGAC CTCTCTTCTG 
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855111.1
    CDS121..3909
    Translation

    Target ORF information:

    RefSeq Version XM_024999343.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999343.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTGTTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCGAGTG GCAACTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTCCCTGTGA GAACAACATC ACGGTCCCCT GTCCTGTCCC GCCGGGATTC CCCACTGCAG 
    GGTAGTGGGC AGCAGAGTAA CCAGGCTGGT CAGAGAAACT CCACCAGTAG CATCGAGCCG 
    AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG GCGCCCAGGC CGGGCAGTGG CAGCTCCTCG 
    GGGTCCAGCA ACTCGGGGTC CCAGCCCGGC TCCCACCCAG GGTCTCAGAG TGGCTCCGGG 
    GAGCGCTTCA GAGTGAGATC ATCATCCAAA TCTGAAGGCT CGCCATCTCA GCGCCTTGAA 
    AACGCAGCAA AAAAGCCTGA AGATAAAAAG GAAGTGTTTA GACCTCTCAA GCCTGCTGAC 
    CTGACGGCTC TGGCCAAAGA GCTGCGGGCC GTGGAGGATG TGCGGCCGCC GCACAAGGTG 
    ACAGACTACT CCTCCTCCAG CGAGGAGTCG GGGACAACGG ACGAGGAGGA CGACGACGTG 
    GAGCAGGAGG GGGCCGAGGA GCCTACGTCT GGCCCGGAGG ACACCAGAGC TGCATCATCT 
    CTGAACTTGA GCAATGGTGA AACAGAGTCC GTGAAAACCA TGGTTGTCCA TGACGACGTG 
    GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA GAGGGCACGC TGATCGTCCG CCAGAGTGCA 
    GTTGACCAAA AGCGCGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC 
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCTTCCCCA 
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTACT 
    AATGAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GCGGGACAAC AGTGACTTCT 
    GTGGTGGGGT TTTCCTGTGA TGGAATGAGA CCTGAGGCCA TGAGGCAAGA TCCTACGCGG 
    AAGGGCTCAG TGGTCAATGT CAATCCCACC AATACTCGGC CCCAGAGCGA CACCCCAGAG 
    ATTCGCAAAT ACAAGAAGAG GTTTAACTCC GAGATCCTGT GCGCAGCCTT GTGGGGAGTG 
    AACTTGTTGG TGGGTACCGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
    GTGTATCCTC TGATCAACAG AAGGCGATTT CAGCAGATGG ACGTCCTGGA AGGCTTGAAC 
    GTCTTAGTGA CGATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
    AGAAATAAAA TACTTCACAA CGACCCGGAG GTTGAGAAGA AGCAGGGCTG GACGACAGTG 
    GGGGACCTGG AAGGCTGTGT GCATTATAAA GTTGTAAAAT ATGAAAGGAT CAAATTTCTG 
    GTCATTGCTT TGAAGAACTC TGTGGAAGTC TACGCATGGG CACCCAAGCC ATATCACAAA 
    TTCATGGCCT TTAAGTCATT TGGTGAGCTG GTGCACAAGC CGTTGCTGGT GGATCTCACT 
    GTTGAGGAAG GCCAGAGGCT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
    GATGTGGACT CAGGATCGGT CTATGACATT TACCTACCAA CACACATCCA ATGTAGCATC 
    AAACCCCACG CAATCATCAT CCTCCCCAAC ACAGATGGCA TGGAGCTCCT GGTGTGCTAT 
    GAAGATGAAG GGGTTTACGT GAACACGTAC GGGAGGATCA CCAAGGACGT GGTTCTGCAG 
    TGGGGAGAGA TGCCAACGTC TGTAGCATAT ATTCGATCCA ATCAGACGAT GGGCTGGGGA 
    GAAAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGCCACT TGGATGGCGT GTTTATGCAT 
    AAAAGGGCTC AAAGGCTGAA GTTCTTGTGT GAACGTAATG ACAAGGTGTT CTTTGCCTCC 
    GTCCGGTCTG GGGGCAGCAG CCAGGTTTAC TTCATGACTC TCGGCAGGAC CTCTCTTCTG 
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999344.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855112.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X23, mRNA.
    Price & Availability↑
    CloneID OBa220767
    Clone ID Related Accession (Same CDS sequence) XM_024999344.1
    Accession Version XM_024999344.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTCCCTGTG 
    AGAACAACAT CACGGTCCCC TGTCCTGTCC CGCCGGGATT CCCCACTGCA GGGTAGTGGG 
    CAGCAGAGTA ACCAGGCTGG TCAGAGAAAC TCCACCAGTA GCATCGAGCC GAGGCTGCTC 
    TGGGAGAGAG TGGAGAAGCT GGCGCCCAGG CCGGGCAGTG GCAGCTCCTC GGGGTCCAGC 
    AACTCGGGGT CCCAGCCCGG CTCCCACCCA GGGTCTCAGA GTGGCTCCGG GGAGCGCTTC 
    AGAGTGAGAT CATCATCCAA ATCTGAAGGC TCGCCATCTC AGCGCCTTGA AAACGCAGCA 
    AAAAAGCCTG AAGATAAAAA GGAAGTGTTT AGACCTCTCA AGCCTGCTGG TGAAGTGGAC 
    CTGACGGCTC TGGCCAAAGA GCTGCGGGCC GTGGAGGATG TGCGGCCGCC GCACAAGGTG 
    ACAGACTACT CCTCCTCCAG CGAGGAGTCG GGGACAACGG ACGAGGAGGA CGACGACGTG 
    GAGCAGGAGG GGGCCGAGGA GCCTACGTCT GGCCCGGAGG ACACCAGAGC TGCATCATCT 
    CTGAACTTGA GCAATGGTGA AACAGAGTCC GTGAAAACCA TGGTTGTCCA TGACGACGTG 
    GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA GAGGGCACGC TGATCGTCCG CCAGAGTGCA 
    GTTGACCAAA AGCGCGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC 
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCTTCCCCA 
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTACT 
    AATGAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GCGGGACAAC AGTGACTTCT 
    GTGGTGGGGT TTTCCTGTGA TGGAATGAGA CCTGAGGCCA TGAGGCAAGA TCCTACGCGG 
    AAGGGCTCAG TGGTCAATGT CAATCCCACC AATACTCGGC CCCAGAGCGA CACCCCAGAG 
    ATTCGCAAAT ACAAGAAGAG GTTTAACTCC GAGATCCTGT GCGCAGCCTT GTGGGGAGTG 
    AACTTGTTGG TGGGTACCGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
    GTGTATCCTC TGATCAACAG AAGGCGATTT CAGCAGATGG ACGTCCTGGA AGGCTTGAAC 
    GTCTTAGTGA CGATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
    AGAAATAAAA TACTTCACAA CGACCCGGAG GTTGAGAAGA AGCAGGGCTG GACGACAGTG 
    GGGGACCTGG AAGGCTGTGT GCATTATAAA GTTGTAAAAT ATGAAAGGAT CAAATTTCTG 
    GTCATTGCTT TGAAGAACTC TGTGGAAGTC TACGCATGGG CACCCAAGCC ATATCACAAA 
    TTCATGGCCT TTAAGTCATT TGGTGAGCTG GTGCACAAGC CGTTGCTGGT GGATCTCACT 
    GTTGAGGAAG GCCAGAGGCT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
    GATGTGGACT CAGGATCGGT CTATGACATT TACCTACCAA CACACGTAAG AAAGAACCCA 
    CACTCTATGA TCCAATGTAG CATCAAACCC CACGCAATCA TCATCCTCCC CAACACAGAT 
    GGCATGGAGC TCCTGGTGTG CTATGAAGAT GAAGGGGTTT ACGTGAACAC GTACGGGAGG 
    ATCACCAAGG ACGTGGTTCT GCAGTGGGGA GAGATGCCAA CGTCTGTAGC ATATATTCGA 
    TCCAATCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGATCTGT GGAAACTGGC 
    CACTTGGATG GCGTGTTTAT GCATAAAAGG GCTCAAAGGC TGAAGTTCTT GTGTGAACGT 
    AATGACAAGG TGTTCTTTGC CTCCGTCCGG TCTGGGGGCA GCAGCCAGGT TTACTTCATG 
    ACTCTCGGCA GGACCTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855112.1
    CDS121..3873
    Translation

    Target ORF information:

    RefSeq Version XM_024999344.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999344.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTCCCTGTG 
    AGAACAACAT CACGGTCCCC TGTCCTGTCC CGCCGGGATT CCCCACTGCA GGGTAGTGGG 
    CAGCAGAGTA ACCAGGCTGG TCAGAGAAAC TCCACCAGTA GCATCGAGCC GAGGCTGCTC 
    TGGGAGAGAG TGGAGAAGCT GGCGCCCAGG CCGGGCAGTG GCAGCTCCTC GGGGTCCAGC 
    AACTCGGGGT CCCAGCCCGG CTCCCACCCA GGGTCTCAGA GTGGCTCCGG GGAGCGCTTC 
    AGAGTGAGAT CATCATCCAA ATCTGAAGGC TCGCCATCTC AGCGCCTTGA AAACGCAGCA 
    AAAAAGCCTG AAGATAAAAA GGAAGTGTTT AGACCTCTCA AGCCTGCTGG TGAAGTGGAC 
    CTGACGGCTC TGGCCAAAGA GCTGCGGGCC GTGGAGGATG TGCGGCCGCC GCACAAGGTG 
    ACAGACTACT CCTCCTCCAG CGAGGAGTCG GGGACAACGG ACGAGGAGGA CGACGACGTG 
    GAGCAGGAGG GGGCCGAGGA GCCTACGTCT GGCCCGGAGG ACACCAGAGC TGCATCATCT 
    CTGAACTTGA GCAATGGTGA AACAGAGTCC GTGAAAACCA TGGTTGTCCA TGACGACGTG 
    GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA GAGGGCACGC TGATCGTCCG CCAGAGTGCA 
    GTTGACCAAA AGCGCGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC 
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCTTCCCCA 
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTACT 
    AATGAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GCGGGACAAC AGTGACTTCT 
    GTGGTGGGGT TTTCCTGTGA TGGAATGAGA CCTGAGGCCA TGAGGCAAGA TCCTACGCGG 
    AAGGGCTCAG TGGTCAATGT CAATCCCACC AATACTCGGC CCCAGAGCGA CACCCCAGAG 
    ATTCGCAAAT ACAAGAAGAG GTTTAACTCC GAGATCCTGT GCGCAGCCTT GTGGGGAGTG 
    AACTTGTTGG TGGGTACCGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
    GTGTATCCTC TGATCAACAG AAGGCGATTT CAGCAGATGG ACGTCCTGGA AGGCTTGAAC 
    GTCTTAGTGA CGATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
    AGAAATAAAA TACTTCACAA CGACCCGGAG GTTGAGAAGA AGCAGGGCTG GACGACAGTG 
    GGGGACCTGG AAGGCTGTGT GCATTATAAA GTTGTAAAAT ATGAAAGGAT CAAATTTCTG 
    GTCATTGCTT TGAAGAACTC TGTGGAAGTC TACGCATGGG CACCCAAGCC ATATCACAAA 
    TTCATGGCCT TTAAGTCATT TGGTGAGCTG GTGCACAAGC CGTTGCTGGT GGATCTCACT 
    GTTGAGGAAG GCCAGAGGCT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
    GATGTGGACT CAGGATCGGT CTATGACATT TACCTACCAA CACACGTAAG AAAGAACCCA 
    CACTCTATGA TCCAATGTAG CATCAAACCC CACGCAATCA TCATCCTCCC CAACACAGAT 
    GGCATGGAGC TCCTGGTGTG CTATGAAGAT GAAGGGGTTT ACGTGAACAC GTACGGGAGG 
    ATCACCAAGG ACGTGGTTCT GCAGTGGGGA GAGATGCCAA CGTCTGTAGC ATATATTCGA 
    TCCAATCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGATCTGT GGAAACTGGC 
    CACTTGGATG GCGTGTTTAT GCATAAAAGG GCTCAAAGGC TGAAGTTCTT GTGTGAACGT 
    AATGACAAGG TGTTCTTTGC CTCCGTCCGG TCTGGGGGCA GCAGCCAGGT TTACTTCATG 
    ACTCTCGGCA GGACCTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999345.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855113.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X24, mRNA.
    Price & Availability↑
    CloneID OBa220768
    Clone ID Related Accession (Same CDS sequence) XM_024999345.1
    Accession Version XM_024999345.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTCCCTGTG 
    AGAACAACAT CACGGTCCCC TGTCCTGTCC CGCCGGGATT CCCCACTGCA GGGTAGTGGG 
    CAGCAGAGTA ACCAGGCTGG TCAGAGAAAC TCCACCAGCA TCGAGCCGAG GCTGCTCTGG 
    GAGAGAGTGG AGAAGCTGGC GCCCAGGCCG GGCAGTGGCA GCTCCTCGGG GTCCAGCAAC 
    TCGGGGTCCC AGCCCGGCTC CCACCCAGGG TCTCAGAGTG GCTCCGGGGA GCGCTTCAGA 
    GTGAGATCAT CATCCAAATC TGAAGGCTCG CCATCTCAGC GCCTTGAAAA CGCAGCAAAA 
    AAGCCTGAAG ATAAAAAGGA AGTGTTTAGA CCTCTCAAGC CTGCTGGTGA AGTGGACCTG 
    ACGGCTCTGG CCAAAGAGCT GCGGGCCGTG GAGGATGTGC GGCCGCCGCA CAAGGTGACA 
    GACTACTCCT CCTCCAGCGA GGAGTCGGGG ACAACGGACG AGGAGGACGA CGACGTGGAG 
    CAGGAGGGGG CCGAGGAGCC TACGTCTGGC CCGGAGGACA CCAGAGCTGC ATCATCTCTG 
    AACTTGAGCA ATGGTGAAAC AGAGTCCGTG AAAACCATGG TTGTCCATGA CGACGTGGAG 
    AGTGAACCAG CCCTGACCCC GTCCAAAGAG GGCACGCTGA TCGTCCGCCA GAGTGCAGTT 
    GACCAAAAGC GCGCCAGCCA TCATGAGAGC AATGGCTTTG CGGGTCGCAT TCACCTCTTG 
    CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCCTC TTCCCCATCC 
    TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTACTAAT 
    GAGACTCAGT CCGCTAGTAA CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
    TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGCG GGACAACAGT GACTTCTGTG 
    GTGGGGTTTT CCTGTGATGG AATGAGACCT GAGGCCATGA GGCAAGATCC TACGCGGAAG 
    GGCTCAGTGG TCAATGTCAA TCCCACCAAT ACTCGGCCCC AGAGCGACAC CCCAGAGATT 
    CGCAAATACA AGAAGAGGTT TAACTCCGAG ATCCTGTGCG CAGCCTTGTG GGGAGTGAAC 
    TTGTTGGTGG GTACCGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTG 
    TATCCTCTGA TCAACAGAAG GCGATTTCAG CAGATGGACG TCCTGGAAGG CTTGAACGTC 
    TTAGTGACGA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA 
    AATAAAATAC TTCACAACGA CCCGGAGGTT GAGAAGAAGC AGGGCTGGAC GACAGTGGGG 
    GACCTGGAAG GCTGTGTGCA TTATAAAGTT GTAAAATATG AAAGGATCAA ATTTCTGGTC 
    ATTGCTTTGA AGAACTCTGT GGAAGTCTAC GCATGGGCAC CCAAGCCATA TCACAAATTC 
    ATGGCCTTTA AGTCATTTGG TGAGCTGGTG CACAAGCCGT TGCTGGTGGA TCTCACTGTT 
    GAGGAAGGCC AGAGGCTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT 
    GTGGACTCAG GATCGGTCTA TGACATTTAC CTACCAACAC ACGTAAGAAA GAACCCACAC 
    TCTATGATCC AATGTAGCAT CAAACCCCAC GCAATCATCA TCCTCCCCAA CACAGATGGC 
    ATGGAGCTCC TGGTGTGCTA TGAAGATGAA GGGGTTTACG TGAACACGTA CGGGAGGATC 
    ACCAAGGACG TGGTTCTGCA GTGGGGAGAG ATGCCAACGT CTGTAGCATA TATTCGATCC 
    AATCAGACGA TGGGCTGGGG AGAAAAGGCC ATAGAGATCC GATCTGTGGA AACTGGCCAC 
    TTGGATGGCG TGTTTATGCA TAAAAGGGCT CAAAGGCTGA AGTTCTTGTG TGAACGTAAT 
    GACAAGGTGT TCTTTGCCTC CGTCCGGTCT GGGGGCAGCA GCCAGGTTTA CTTCATGACT 
    CTCGGCAGGA CCTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855113.1
    CDS121..3870
    Translation

    Target ORF information:

    RefSeq Version XM_024999345.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999345.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTCCCTGTG 
    AGAACAACAT CACGGTCCCC TGTCCTGTCC CGCCGGGATT CCCCACTGCA GGGTAGTGGG 
    CAGCAGAGTA ACCAGGCTGG TCAGAGAAAC TCCACCAGCA TCGAGCCGAG GCTGCTCTGG 
    GAGAGAGTGG AGAAGCTGGC GCCCAGGCCG GGCAGTGGCA GCTCCTCGGG GTCCAGCAAC 
    TCGGGGTCCC AGCCCGGCTC CCACCCAGGG TCTCAGAGTG GCTCCGGGGA GCGCTTCAGA 
    GTGAGATCAT CATCCAAATC TGAAGGCTCG CCATCTCAGC GCCTTGAAAA CGCAGCAAAA 
    AAGCCTGAAG ATAAAAAGGA AGTGTTTAGA CCTCTCAAGC CTGCTGGTGA AGTGGACCTG 
    ACGGCTCTGG CCAAAGAGCT GCGGGCCGTG GAGGATGTGC GGCCGCCGCA CAAGGTGACA 
    GACTACTCCT CCTCCAGCGA GGAGTCGGGG ACAACGGACG AGGAGGACGA CGACGTGGAG 
    CAGGAGGGGG CCGAGGAGCC TACGTCTGGC CCGGAGGACA CCAGAGCTGC ATCATCTCTG 
    AACTTGAGCA ATGGTGAAAC AGAGTCCGTG AAAACCATGG TTGTCCATGA CGACGTGGAG 
    AGTGAACCAG CCCTGACCCC GTCCAAAGAG GGCACGCTGA TCGTCCGCCA GAGTGCAGTT 
    GACCAAAAGC GCGCCAGCCA TCATGAGAGC AATGGCTTTG CGGGTCGCAT TCACCTCTTG 
    CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCCTC TTCCCCATCC 
    TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTACTAAT 
    GAGACTCAGT CCGCTAGTAA CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
    TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGCG GGACAACAGT GACTTCTGTG 
    GTGGGGTTTT CCTGTGATGG AATGAGACCT GAGGCCATGA GGCAAGATCC TACGCGGAAG 
    GGCTCAGTGG TCAATGTCAA TCCCACCAAT ACTCGGCCCC AGAGCGACAC CCCAGAGATT 
    CGCAAATACA AGAAGAGGTT TAACTCCGAG ATCCTGTGCG CAGCCTTGTG GGGAGTGAAC 
    TTGTTGGTGG GTACCGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTG 
    TATCCTCTGA TCAACAGAAG GCGATTTCAG CAGATGGACG TCCTGGAAGG CTTGAACGTC 
    TTAGTGACGA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA 
    AATAAAATAC TTCACAACGA CCCGGAGGTT GAGAAGAAGC AGGGCTGGAC GACAGTGGGG 
    GACCTGGAAG GCTGTGTGCA TTATAAAGTT GTAAAATATG AAAGGATCAA ATTTCTGGTC 
    ATTGCTTTGA AGAACTCTGT GGAAGTCTAC GCATGGGCAC CCAAGCCATA TCACAAATTC 
    ATGGCCTTTA AGTCATTTGG TGAGCTGGTG CACAAGCCGT TGCTGGTGGA TCTCACTGTT 
    GAGGAAGGCC AGAGGCTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT 
    GTGGACTCAG GATCGGTCTA TGACATTTAC CTACCAACAC ACGTAAGAAA GAACCCACAC 
    TCTATGATCC AATGTAGCAT CAAACCCCAC GCAATCATCA TCCTCCCCAA CACAGATGGC 
    ATGGAGCTCC TGGTGTGCTA TGAAGATGAA GGGGTTTACG TGAACACGTA CGGGAGGATC 
    ACCAAGGACG TGGTTCTGCA GTGGGGAGAG ATGCCAACGT CTGTAGCATA TATTCGATCC 
    AATCAGACGA TGGGCTGGGG AGAAAAGGCC ATAGAGATCC GATCTGTGGA AACTGGCCAC 
    TTGGATGGCG TGTTTATGCA TAAAAGGGCT CAAAGGCTGA AGTTCTTGTG TGAACGTAAT 
    GACAAGGTGT TCTTTGCCTC CGTCCGGTCT GGGGGCAGCA GCCAGGTTTA CTTCATGACT 
    CTCGGCAGGA CCTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999346.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855114.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X25, mRNA.
    Price & Availability↑
    CloneID OBa220769
    Clone ID Related Accession (Same CDS sequence) XM_024999346.1
    Accession Version XM_024999346.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3744bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTCCCTGTG 
    AGAACAACAT CACGGTCCCC TGTCCTGTCC CGCCGGGATT CCCCACTGCA GGGTAGTGGG 
    CAGCAGAGTA ACCAGGCTGG TCAGAGAAAC TCCACCAGTA GCATCGAGCC GAGGCTGCTC 
    TGGGAGAGAG TGGAGAAGCT GGCGCCCAGG CCGGGCAGTG GCAGCTCCTC GGGGTCCAGC 
    AACTCGGGGT CCCAGCCCGG CTCCCACCCA GGGTCTCAGA GTGGCTCCGG GGAGCGCTTC 
    AGAGTGAGAT CATCATCCAA ATCTGAAGGC TCGCCATCTC AGCGCCTTGA AAACGCAGCA 
    AAAAAGCCTG AAGATAAAAA GGAAGTGTTT AGACCTCTCA AGCCTGCTGA CCTGACGGCT 
    CTGGCCAAAG AGCTGCGGGC CGTGGAGGAT GTGCGGCCGC CGCACAAGGT GACAGACTAC 
    TCCTCCTCCA GCGAGGAGTC GGGGACAACG GACGAGGAGG ACGACGACGT GGAGCAGGAG 
    GGGGCCGAGG AGCCTACGTC TGGCCCGGAG GACACCAGAG CTGCATCATC TCTGAACTTG 
    AGCAATGGTG AAACAGAGTC CGTGAAAACC ATGGTTGTCC ATGACGACGT GGAGAGTGAA 
    CCAGCCCTGA CCCCGTCCAA AGAGGGCACG CTGATCGTCC GCCAGAGTGC AGTTGACCAA 
    AAGCGCGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT 
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCTTCCCC ATCCTCCAGC 
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTAC TAATGAGACT 
    CAGTCCGCTA GTAACACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGGACAA CAGTGACTTC TGTGGTGGGG 
    TTTTCCTGTG ATGGAATGAG ACCTGAGGCC ATGAGGCAAG ATCCTACGCG GAAGGGCTCA 
    GTGGTCAATG TCAATCCCAC CAATACTCGG CCCCAGAGCG ACACCCCAGA GATTCGCAAA 
    TACAAGAAGA GGTTTAACTC CGAGATCCTG TGCGCAGCCT TGTGGGGAGT GAACTTGTTG 
    GTGGGTACCG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTGTATCCT 
    CTGATCAACA GAAGGCGATT TCAGCAGATG GACGTCCTGG AAGGCTTGAA CGTCTTAGTG 
    ACGATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
    ATACTTCACA ACGACCCGGA GGTTGAGAAG AAGCAGGGCT GGACGACAGT GGGGGACCTG 
    GAAGGCTGTG TGCATTATAA AGTTGTAAAA TATGAAAGGA TCAAATTTCT GGTCATTGCT 
    TTGAAGAACT CTGTGGAAGT CTACGCATGG GCACCCAAGC CATATCACAA ATTCATGGCC 
    TTTAAGTCAT TTGGTGAGCT GGTGCACAAG CCGTTGCTGG TGGATCTCAC TGTTGAGGAA 
    GGCCAGAGGC TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAC 
    TCAGGATCGG TCTATGACAT TTACCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG 
    ATCCAATGTA GCATCAAACC CCACGCAATC ATCATCCTCC CCAACACAGA TGGCATGGAG 
    CTCCTGGTGT GCTATGAAGA TGAAGGGGTT TACGTGAACA CGTACGGGAG GATCACCAAG 
    GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACGTCTGTAG CATATATTCG ATCCAATCAG 
    ACGATGGGCT GGGGAGAAAA GGCCATAGAG ATCCGATCTG TGGAAACTGG CCACTTGGAT 
    GGCGTGTTTA TGCATAAAAG GGCTCAAAGG CTGAAGTTCT TGTGTGAACG TAATGACAAG 
    GTGTTCTTTG CCTCCGTCCG GTCTGGGGGC AGCAGCCAGG TTTACTTCAT GACTCTCGGC 
    AGGACCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855114.1
    CDS120..3863
    Translation

    Target ORF information:

    RefSeq Version XM_024999346.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999346.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTCCCTGTG 
    AGAACAACAT CACGGTCCCC TGTCCTGTCC CGCCGGGATT CCCCACTGCA GGGTAGTGGG 
    CAGCAGAGTA ACCAGGCTGG TCAGAGAAAC TCCACCAGTA GCATCGAGCC GAGGCTGCTC 
    TGGGAGAGAG TGGAGAAGCT GGCGCCCAGG CCGGGCAGTG GCAGCTCCTC GGGGTCCAGC 
    AACTCGGGGT CCCAGCCCGG CTCCCACCCA GGGTCTCAGA GTGGCTCCGG GGAGCGCTTC 
    AGAGTGAGAT CATCATCCAA ATCTGAAGGC TCGCCATCTC AGCGCCTTGA AAACGCAGCA 
    AAAAAGCCTG AAGATAAAAA GGAAGTGTTT AGACCTCTCA AGCCTGCTGA CCTGACGGCT 
    CTGGCCAAAG AGCTGCGGGC CGTGGAGGAT GTGCGGCCGC CGCACAAGGT GACAGACTAC 
    TCCTCCTCCA GCGAGGAGTC GGGGACAACG GACGAGGAGG ACGACGACGT GGAGCAGGAG 
    GGGGCCGAGG AGCCTACGTC TGGCCCGGAG GACACCAGAG CTGCATCATC TCTGAACTTG 
    AGCAATGGTG AAACAGAGTC CGTGAAAACC ATGGTTGTCC ATGACGACGT GGAGAGTGAA 
    CCAGCCCTGA CCCCGTCCAA AGAGGGCACG CTGATCGTCC GCCAGAGTGC AGTTGACCAA 
    AAGCGCGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT 
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCTTCCCC ATCCTCCAGC 
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTAC TAATGAGACT 
    CAGTCCGCTA GTAACACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGGACAA CAGTGACTTC TGTGGTGGGG 
    TTTTCCTGTG ATGGAATGAG ACCTGAGGCC ATGAGGCAAG ATCCTACGCG GAAGGGCTCA 
    GTGGTCAATG TCAATCCCAC CAATACTCGG CCCCAGAGCG ACACCCCAGA GATTCGCAAA 
    TACAAGAAGA GGTTTAACTC CGAGATCCTG TGCGCAGCCT TGTGGGGAGT GAACTTGTTG 
    GTGGGTACCG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTGTATCCT 
    CTGATCAACA GAAGGCGATT TCAGCAGATG GACGTCCTGG AAGGCTTGAA CGTCTTAGTG 
    ACGATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
    ATACTTCACA ACGACCCGGA GGTTGAGAAG AAGCAGGGCT GGACGACAGT GGGGGACCTG 
    GAAGGCTGTG TGCATTATAA AGTTGTAAAA TATGAAAGGA TCAAATTTCT GGTCATTGCT 
    TTGAAGAACT CTGTGGAAGT CTACGCATGG GCACCCAAGC CATATCACAA ATTCATGGCC 
    TTTAAGTCAT TTGGTGAGCT GGTGCACAAG CCGTTGCTGG TGGATCTCAC TGTTGAGGAA 
    GGCCAGAGGC TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAC 
    TCAGGATCGG TCTATGACAT TTACCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG 
    ATCCAATGTA GCATCAAACC CCACGCAATC ATCATCCTCC CCAACACAGA TGGCATGGAG 
    CTCCTGGTGT GCTATGAAGA TGAAGGGGTT TACGTGAACA CGTACGGGAG GATCACCAAG 
    GACGTGGTTC TGCAGTGGGG AGAGATGCCA ACGTCTGTAG CATATATTCG ATCCAATCAG 
    ACGATGGGCT GGGGAGAAAA GGCCATAGAG ATCCGATCTG TGGAAACTGG CCACTTGGAT 
    GGCGTGTTTA TGCATAAAAG GGCTCAAAGG CTGAAGTTCT TGTGTGAACG TAATGACAAG 
    GTGTTCTTTG CCTCCGTCCG GTCTGGGGGC AGCAGCCAGG TTTACTTCAT GACTCTCGGC 
    AGGACCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999347.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855115.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X26, mRNA.
    Price & Availability↑
    CloneID OBa220770
    Clone ID Related Accession (Same CDS sequence) XM_024999347.1
    Accession Version XM_024999347.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3687bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTCCCTG TGAGAACAAC ATCACGGTCC 
    CCTGTCCTGT CCCGCCGGGA TTCCCCACTG CAGGGTAGTG GGCAGCAGAG TAACCAGGCT 
    GGTCAGAGAA ACTCCACCAG CATCGAGCCG AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG 
    GCGCCCAGGC CGGGCAGTGG CAGCTCCTCG GGGTCCAGCA ACTCGGGGTC CCAGCCCGGC 
    TCCCACCCAG GGTCTCAGAG TGGCTCCGGG GAGCGCTTCA GAGTGAGATC ATCATCCAAA 
    TCTGAAGGCT CGCCATCTCA GCGCCTTGAA AACGCAGCAA AAAAGCCTGA AGATAAAAAG 
    GAAGTGTTTA GACCTCTCAA GCCTGCTGAC CTGACGGCTC TGGCCAAAGA GCTGCGGGCC 
    GTGGAGGATG TGCGGCCGCC GCACAAGGTG ACAGACTACT CCTCCTCCAG CGAGGAGTCG 
    GGGACAACGG ACGAGGAGGA CGACGACGTG GAGCAGGAGG GGGCCGAGGA GCCTACGTCT 
    GGCCCGGAGG ACACCAGAGC TGCATCATCT CTGAACTTGA GCAATGGTGA AACAGAGTCC 
    GTGAAAACCA TGGTTGTCCA TGACGACGTG GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA 
    GAGGGCACGC TGATCGTCCG CCAGACTCAG TCCGCTAGTA ACACACTCCA GAAACACAAA 
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGATTAC TACAGATTTC TCCATCTAGC 
    GGGACAACAG TGACTTCTGT GGTGGGGTTT TCCTGTGATG GAATGAGACC TGAGGCCATG 
    AGGCAAGATC CTACGCGGAA GGGCTCAGTG GTCAATGTCA ATCCCACCAA TACTCGGCCC 
    CAGAGCGACA CCCCAGAGAT TCGCAAATAC AAGAAGAGGT TTAACTCCGA GATCCTGTGC 
    GCAGCCTTGT GGGGAGTGAA CTTGTTGGTG GGTACCGAGA GTGGCCTGAT GCTGCTGGAC 
    AGAAGTGGCC AAGGGAAGGT GTATCCTCTG ATCAACAGAA GGCGATTTCA GCAGATGGAC 
    GTCCTGGAAG GCTTGAACGT CTTAGTGACG ATATCTGGCA AAAAGGATAA GTTACGTGTC 
    TACTATTTGT CCTGGTTAAG AAATAAAATA CTTCACAACG ACCCGGAGGT TGAGAAGAAG 
    CAGGGCTGGA CGACAGTGGG GGACCTGGAA GGCTGTGTGC ATTATAAAGT TGTAAAATAT 
    GAAAGGATCA AATTTCTGGT CATTGCTTTG AAGAACTCTG TGGAAGTCTA CGCATGGGCA 
    CCCAAGCCAT ATCACAAATT CATGGCCTTT AAGTCATTTG GTGAGCTGGT GCACAAGCCG 
    TTGCTGGTGG ATCTCACTGT TGAGGAAGGC CAGAGGCTGA AAGTGATCTA TGGATCCTGT 
    GCTGGATTCC ATGCTGTTGA TGTGGACTCA GGATCGGTCT ATGACATTTA CCTACCAACA 
    CACATCCAAT GTAGCATCAA ACCCCACGCA ATCATCATCC TCCCCAACAC AGATGGCATG 
    GAGCTCCTGG TGTGCTATGA AGATGAAGGG GTTTACGTGA ACACGTACGG GAGGATCACC 
    AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCAACGTCTG TAGCATATAT TCGATCCAAT 
    CAGACGATGG GCTGGGGAGA AAAGGCCATA GAGATCCGAT CTGTGGAAAC TGGCCACTTG 
    GATGGCGTGT TTATGCATAA AAGGGCTCAA AGGCTGAAGT TCTTGTGTGA ACGTAATGAC 
    AAGGTGTTCT TTGCCTCCGT CCGGTCTGGG GGCAGCAGCC AGGTTTACTT CATGACTCTC 
    GGCAGGACCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855115.1
    CDS120..3806
    Translation

    Target ORF information:

    RefSeq Version XM_024999347.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999347.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGTCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGTGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTG 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCGA GTGGCAACTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTCCCTG TGAGAACAAC ATCACGGTCC 
    CCTGTCCTGT CCCGCCGGGA TTCCCCACTG CAGGGTAGTG GGCAGCAGAG TAACCAGGCT 
    GGTCAGAGAA ACTCCACCAG CATCGAGCCG AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG 
    GCGCCCAGGC CGGGCAGTGG CAGCTCCTCG GGGTCCAGCA ACTCGGGGTC CCAGCCCGGC 
    TCCCACCCAG GGTCTCAGAG TGGCTCCGGG GAGCGCTTCA GAGTGAGATC ATCATCCAAA 
    TCTGAAGGCT CGCCATCTCA GCGCCTTGAA AACGCAGCAA AAAAGCCTGA AGATAAAAAG 
    GAAGTGTTTA GACCTCTCAA GCCTGCTGAC CTGACGGCTC TGGCCAAAGA GCTGCGGGCC 
    GTGGAGGATG TGCGGCCGCC GCACAAGGTG ACAGACTACT CCTCCTCCAG CGAGGAGTCG 
    GGGACAACGG ACGAGGAGGA CGACGACGTG GAGCAGGAGG GGGCCGAGGA GCCTACGTCT 
    GGCCCGGAGG ACACCAGAGC TGCATCATCT CTGAACTTGA GCAATGGTGA AACAGAGTCC 
    GTGAAAACCA TGGTTGTCCA TGACGACGTG GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA 
    GAGGGCACGC TGATCGTCCG CCAGACTCAG TCCGCTAGTA ACACACTCCA GAAACACAAA 
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGATTAC TACAGATTTC TCCATCTAGC 
    GGGACAACAG TGACTTCTGT GGTGGGGTTT TCCTGTGATG GAATGAGACC TGAGGCCATG 
    AGGCAAGATC CTACGCGGAA GGGCTCAGTG GTCAATGTCA ATCCCACCAA TACTCGGCCC 
    CAGAGCGACA CCCCAGAGAT TCGCAAATAC AAGAAGAGGT TTAACTCCGA GATCCTGTGC 
    GCAGCCTTGT GGGGAGTGAA CTTGTTGGTG GGTACCGAGA GTGGCCTGAT GCTGCTGGAC 
    AGAAGTGGCC AAGGGAAGGT GTATCCTCTG ATCAACAGAA GGCGATTTCA GCAGATGGAC 
    GTCCTGGAAG GCTTGAACGT CTTAGTGACG ATATCTGGCA AAAAGGATAA GTTACGTGTC 
    TACTATTTGT CCTGGTTAAG AAATAAAATA CTTCACAACG ACCCGGAGGT TGAGAAGAAG 
    CAGGGCTGGA CGACAGTGGG GGACCTGGAA GGCTGTGTGC ATTATAAAGT TGTAAAATAT 
    GAAAGGATCA AATTTCTGGT CATTGCTTTG AAGAACTCTG TGGAAGTCTA CGCATGGGCA 
    CCCAAGCCAT ATCACAAATT CATGGCCTTT AAGTCATTTG GTGAGCTGGT GCACAAGCCG 
    TTGCTGGTGG ATCTCACTGT TGAGGAAGGC CAGAGGCTGA AAGTGATCTA TGGATCCTGT 
    GCTGGATTCC ATGCTGTTGA TGTGGACTCA GGATCGGTCT ATGACATTTA CCTACCAACA 
    CACATCCAAT GTAGCATCAA ACCCCACGCA ATCATCATCC TCCCCAACAC AGATGGCATG 
    GAGCTCCTGG TGTGCTATGA AGATGAAGGG GTTTACGTGA ACACGTACGG GAGGATCACC 
    AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCAACGTCTG TAGCATATAT TCGATCCAAT 
    CAGACGATGG GCTGGGGAGA AAAGGCCATA GAGATCCGAT CTGTGGAAAC TGGCCACTTG 
    GATGGCGTGT TTATGCATAA AAGGGCTCAA AGGCTGAAGT TCTTGTGTGA ACGTAATGAC 
    AAGGTGTTCT TTGCCTCCGT CCGGTCTGGG GGCAGCAGCC AGGTTTACTT CATGACTCTC 
    GGCAGGACCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999348.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855116.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X27, mRNA.
    Price & Availability↑
    CloneID OBa220771
    Clone ID Related Accession (Same CDS sequence) XM_024999348.1
    Accession Version XM_024999348.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT ACAGTGGTCC CACCTGGCAT CTCTCAAGAA CAATGTTTCC 
    CCTGTCTCGA GATCCCATTC CTTCAGTGAC CCTTCTCCTC CCAAATTTGC ACACCACCAT 
    CTTCGCTCTC AGGACCCCTG CCCACCTTCC CGCAGTGAGG TGCTCAGTCA GAGTTCTGAC 
    TCTAAGGCGG AGGTGCCCGA CCCAGCCCAG AAGGCTTGGT CTAGATCAGA CAGTGACGAG 
    GTGCCTCCAC GGGTCCCTGT GAGAACAACA TCACGGTCCC CTGTCCTGTC CCGCCGGGAT 
    TCCCCACTGC AGGGTAGTGG GCAGCAGAGT AACCAGGCTG GTCAGAGAAA CTCCACCAGT 
    AGCATCGAGC CGAGGCTGCT CTGGGAGAGA GTGGAGAAGC TGGCGCCCAG GCCGGGCAGT 
    GGCAGCTCCT CGGGGTCCAG CAACTCGGGG TCCCAGCCCG GCTCCCACCC AGGGTCTCAG 
    AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA TCATCATCCA AATCTGAAGG CTCGCCATCT 
    CAGCGCCTTG AAAACGCAGC AAAAAAGCCT GAAGATAAAA AGGAAGTGTT TAGACCTCTC 
    AAGCCTGCTG GTGAAGTGGA CCTGACGGCT CTGGCCAAAG AGCTGCGGGC CGTGGAGGAT 
    GTGCGGCCGC CGCACAAGGT GACAGACTAC TCCTCCTCCA GCGAGGAGTC GGGGACAACG 
    GACGAGGAGG ACGACGACGT GGAGCAGGAG GGGGCCGAGG AGCCTACGTC TGGCCCGGAG 
    GACACCAGAG CTGCATCATC TCTGAACTTG AGCAATGGTG AAACAGAGTC CGTGAAAACC 
    ATGGTTGTCC ATGACGACGT GGAGAGTGAA CCAGCCCTGA CCCCGTCCAA AGAGGGCACG 
    CTGATCGTCC GCCAGACTCA GTCCGCTAGT AACACACTCC AGAAACACAA ATCTTCCTCC 
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGGACAACA 
    GTGACTTCTG TGGTGGGGTT TTCCTGTGAT GGAATGAGAC CTGAGGCCAT GAGGCAAGAT 
    CCTACGCGGA AGGGCTCAGT GGTCAATGTC AATCCCACCA ATACTCGGCC CCAGAGCGAC 
    ACCCCAGAGA TTCGCAAATA CAAGAAGAGG TTTAACTCCG AGATCCTGTG CGCAGCCTTG 
    TGGGGAGTGA ACTTGTTGGT GGGTACCGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC 
    CAAGGGAAGG TGTATCCTCT GATCAACAGA AGGCGATTTC AGCAGATGGA CGTCCTGGAA 
    GGCTTGAACG TCTTAGTGAC GATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG 
    TCCTGGTTAA GAAATAAAAT ACTTCACAAC GACCCGGAGG TTGAGAAGAA GCAGGGCTGG 
    ACGACAGTGG GGGACCTGGA AGGCTGTGTG CATTATAAAG TTGTAAAATA TGAAAGGATC 
    AAATTTCTGG TCATTGCTTT GAAGAACTCT GTGGAAGTCT ACGCATGGGC ACCCAAGCCA 
    TATCACAAAT TCATGGCCTT TAAGTCATTT GGTGAGCTGG TGCACAAGCC GTTGCTGGTG 
    GATCTCACTG TTGAGGAAGG CCAGAGGCTG AAAGTGATCT ATGGATCCTG TGCTGGATTC 
    CATGCTGTTG ATGTGGACTC AGGATCGGTC TATGACATTT ACCTACCAAC ACACATCCAA 
    TGTAGCATCA AACCCCACGC AATCATCATC CTCCCCAACA CAGATGGCAT GGAGCTCCTG 
    GTGTGCTATG AAGATGAAGG GGTTTACGTG AACACGTACG GGAGGATCAC CAAGGACGTG 
    GTTCTGCAGT GGGGAGAGAT GCCAACGTCT GTAGCATATA TTCGATCCAA TCAGACGATG 
    GGCTGGGGAG AAAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGCCACTT GGATGGCGTG 
    TTTATGCATA AAAGGGCTCA AAGGCTGAAG TTCTTGTGTG AACGTAATGA CAAGGTGTTC 
    TTTGCCTCCG TCCGGTCTGG GGGCAGCAGC CAGGTTTACT TCATGACTCT CGGCAGGACC 
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855116.1
    CDS121..3798
    Translation

    Target ORF information:

    RefSeq Version XM_024999348.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999348.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT ACAGTGGTCC CACCTGGCAT CTCTCAAGAA CAATGTTTCC 
    CCTGTCTCGA GATCCCATTC CTTCAGTGAC CCTTCTCCTC CCAAATTTGC ACACCACCAT 
    CTTCGCTCTC AGGACCCCTG CCCACCTTCC CGCAGTGAGG TGCTCAGTCA GAGTTCTGAC 
    TCTAAGGCGG AGGTGCCCGA CCCAGCCCAG AAGGCTTGGT CTAGATCAGA CAGTGACGAG 
    GTGCCTCCAC GGGTCCCTGT GAGAACAACA TCACGGTCCC CTGTCCTGTC CCGCCGGGAT 
    TCCCCACTGC AGGGTAGTGG GCAGCAGAGT AACCAGGCTG GTCAGAGAAA CTCCACCAGT 
    AGCATCGAGC CGAGGCTGCT CTGGGAGAGA GTGGAGAAGC TGGCGCCCAG GCCGGGCAGT 
    GGCAGCTCCT CGGGGTCCAG CAACTCGGGG TCCCAGCCCG GCTCCCACCC AGGGTCTCAG 
    AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA TCATCATCCA AATCTGAAGG CTCGCCATCT 
    CAGCGCCTTG AAAACGCAGC AAAAAAGCCT GAAGATAAAA AGGAAGTGTT TAGACCTCTC 
    AAGCCTGCTG GTGAAGTGGA CCTGACGGCT CTGGCCAAAG AGCTGCGGGC CGTGGAGGAT 
    GTGCGGCCGC CGCACAAGGT GACAGACTAC TCCTCCTCCA GCGAGGAGTC GGGGACAACG 
    GACGAGGAGG ACGACGACGT GGAGCAGGAG GGGGCCGAGG AGCCTACGTC TGGCCCGGAG 
    GACACCAGAG CTGCATCATC TCTGAACTTG AGCAATGGTG AAACAGAGTC CGTGAAAACC 
    ATGGTTGTCC ATGACGACGT GGAGAGTGAA CCAGCCCTGA CCCCGTCCAA AGAGGGCACG 
    CTGATCGTCC GCCAGACTCA GTCCGCTAGT AACACACTCC AGAAACACAA ATCTTCCTCC 
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGGACAACA 
    GTGACTTCTG TGGTGGGGTT TTCCTGTGAT GGAATGAGAC CTGAGGCCAT GAGGCAAGAT 
    CCTACGCGGA AGGGCTCAGT GGTCAATGTC AATCCCACCA ATACTCGGCC CCAGAGCGAC 
    ACCCCAGAGA TTCGCAAATA CAAGAAGAGG TTTAACTCCG AGATCCTGTG CGCAGCCTTG 
    TGGGGAGTGA ACTTGTTGGT GGGTACCGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC 
    CAAGGGAAGG TGTATCCTCT GATCAACAGA AGGCGATTTC AGCAGATGGA CGTCCTGGAA 
    GGCTTGAACG TCTTAGTGAC GATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG 
    TCCTGGTTAA GAAATAAAAT ACTTCACAAC GACCCGGAGG TTGAGAAGAA GCAGGGCTGG 
    ACGACAGTGG GGGACCTGGA AGGCTGTGTG CATTATAAAG TTGTAAAATA TGAAAGGATC 
    AAATTTCTGG TCATTGCTTT GAAGAACTCT GTGGAAGTCT ACGCATGGGC ACCCAAGCCA 
    TATCACAAAT TCATGGCCTT TAAGTCATTT GGTGAGCTGG TGCACAAGCC GTTGCTGGTG 
    GATCTCACTG TTGAGGAAGG CCAGAGGCTG AAAGTGATCT ATGGATCCTG TGCTGGATTC 
    CATGCTGTTG ATGTGGACTC AGGATCGGTC TATGACATTT ACCTACCAAC ACACATCCAA 
    TGTAGCATCA AACCCCACGC AATCATCATC CTCCCCAACA CAGATGGCAT GGAGCTCCTG 
    GTGTGCTATG AAGATGAAGG GGTTTACGTG AACACGTACG GGAGGATCAC CAAGGACGTG 
    GTTCTGCAGT GGGGAGAGAT GCCAACGTCT GTAGCATATA TTCGATCCAA TCAGACGATG 
    GGCTGGGGAG AAAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGCCACTT GGATGGCGTG 
    TTTATGCATA AAAGGGCTCA AAGGCTGAAG TTCTTGTGTG AACGTAATGA CAAGGTGTTC 
    TTTGCCTCCG TCCGGTCTGG GGGCAGCAGC CAGGTTTACT TCATGACTCT CGGCAGGACC 
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999349.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855117.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X28, mRNA.
    Price & Availability↑
    CloneID OBa220772
    Clone ID Related Accession (Same CDS sequence) XM_024999349.1
    Accession Version XM_024999349.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT CCCTGTGAGA ACAACATCAC GGTCCCCTGT CCTGTCCCGC 
    CGGGATTCCC CACTGCAGGG TAGTGGGCAG CAGAGTAACC AGGCTGGTCA GAGAAACTCC 
    ACCAGTAGCA TCGAGCCGAG GCTGCTCTGG GAGAGAGTGG AGAAGCTGGC GCCCAGGCCG 
    GGCAGTGGCA GCTCCTCGGG GTCCAGCAAC TCGGGGTCCC AGCCCGGCTC CCACCCAGGG 
    TCTCAGAGTG GCTCCGGGGA GCGCTTCAGA GTGAGATCAT CATCCAAATC TGAAGGCTCG 
    CCATCTCAGC GCCTTGAAAA CGCAGCAAAA AAGCCTGAAG ATAAAAAGGA AGTGTTTAGA 
    CCTCTCAAGC CTGCTGGTGA AGTGGACCTG ACGGCTCTGG CCAAAGAGCT GCGGGCCGTG 
    GAGGATGTGC GGCCGCCGCA CAAGGTGACA GACTACTCCT CCTCCAGCGA GGAGTCGGGG 
    ACAACGGACG AGGAGGACGA CGACGTGGAG CAGGAGGGGG CCGAGGAGCC TACGTCTGGC 
    CCGGAGGACA CCAGAGCTGC ATCATCTCTG AACTTGAGCA ATGGTGAAAC AGAGTCCGTG 
    AAAACCATGG TTGTCCATGA CGACGTGGAG AGTGAACCAG CCCTGACCCC GTCCAAAGAG 
    GGCACGCTGA TCGTCCGCCA GAGTGCAGTT GACCAAAAGC GCGCCAGCCA TCATGAGAGC 
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
    TCCACTTCCT CCACCTCCTC TTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
    CAGACACCCC AGGACAAGCT CACTACTAAT GAGACTCAGT CCGCTAGTAA CACACTCCAG 
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
    CCATCTAGCG GGACAACAGT GACTTCTGTG GTGGGGTTTT CCTGTGATGG AATGAGACCT 
    GAGGCCATGA GGCAAGATCC TACGCGGAAG GGCTCAGTGG TCAATGTCAA TCCCACCAAT 
    ACTCGGCCCC AGAGCGACAC CCCAGAGATT CGCAAATACA AGAAGAGGTT TAACTCCGAG 
    ATCCTGTGCG CAGCCTTGTG GGGAGTGAAC TTGTTGGTGG GTACCGAGAG TGGCCTGATG 
    CTGCTGGACA GAAGTGGCCA AGGGAAGGTG TATCCTCTGA TCAACAGAAG GCGATTTCAG 
    CAGATGGACG TCCTGGAAGG CTTGAACGTC TTAGTGACGA TATCTGGCAA AAAGGATAAG 
    TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAACGA CCCGGAGGTT 
    GAGAAGAAGC AGGGCTGGAC GACAGTGGGG GACCTGGAAG GCTGTGTGCA TTATAAAGTT 
    GTAAAATATG AAAGGATCAA ATTTCTGGTC ATTGCTTTGA AGAACTCTGT GGAAGTCTAC 
    GCATGGGCAC CCAAGCCATA TCACAAATTC ATGGCCTTTA AGTCATTTGG TGAGCTGGTG 
    CACAAGCCGT TGCTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGCTGAA AGTGATCTAT 
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGACTCAG GATCGGTCTA TGACATTTAC 
    CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATCC AATGTAGCAT CAAACCCCAC 
    GCAATCATCA TCCTCCCCAA CACAGATGGC ATGGAGCTCC TGGTGTGCTA TGAAGATGAA 
    GGGGTTTACG TGAACACGTA CGGGAGGATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG 
    ATGCCAACGT CTGTAGCATA TATTCGATCC AATCAGACGA TGGGCTGGGG AGAAAAGGCC 
    ATAGAGATCC GATCTGTGGA AACTGGCCAC TTGGATGGCG TGTTTATGCA TAAAAGGGCT 
    CAAAGGCTGA AGTTCTTGTG TGAACGTAAT GACAAGGTGT TCTTTGCCTC CGTCCGGTCT 
    GGGGGCAGCA GCCAGGTTTA CTTCATGACT CTCGGCAGGA CCTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855117.1
    CDS121..3780
    Translation

    Target ORF information:

    RefSeq Version XM_024999349.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999349.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT CCCTGTGAGA ACAACATCAC GGTCCCCTGT CCTGTCCCGC 
    CGGGATTCCC CACTGCAGGG TAGTGGGCAG CAGAGTAACC AGGCTGGTCA GAGAAACTCC 
    ACCAGTAGCA TCGAGCCGAG GCTGCTCTGG GAGAGAGTGG AGAAGCTGGC GCCCAGGCCG 
    GGCAGTGGCA GCTCCTCGGG GTCCAGCAAC TCGGGGTCCC AGCCCGGCTC CCACCCAGGG 
    TCTCAGAGTG GCTCCGGGGA GCGCTTCAGA GTGAGATCAT CATCCAAATC TGAAGGCTCG 
    CCATCTCAGC GCCTTGAAAA CGCAGCAAAA AAGCCTGAAG ATAAAAAGGA AGTGTTTAGA 
    CCTCTCAAGC CTGCTGGTGA AGTGGACCTG ACGGCTCTGG CCAAAGAGCT GCGGGCCGTG 
    GAGGATGTGC GGCCGCCGCA CAAGGTGACA GACTACTCCT CCTCCAGCGA GGAGTCGGGG 
    ACAACGGACG AGGAGGACGA CGACGTGGAG CAGGAGGGGG CCGAGGAGCC TACGTCTGGC 
    CCGGAGGACA CCAGAGCTGC ATCATCTCTG AACTTGAGCA ATGGTGAAAC AGAGTCCGTG 
    AAAACCATGG TTGTCCATGA CGACGTGGAG AGTGAACCAG CCCTGACCCC GTCCAAAGAG 
    GGCACGCTGA TCGTCCGCCA GAGTGCAGTT GACCAAAAGC GCGCCAGCCA TCATGAGAGC 
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
    TCCACTTCCT CCACCTCCTC TTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
    CAGACACCCC AGGACAAGCT CACTACTAAT GAGACTCAGT CCGCTAGTAA CACACTCCAG 
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
    CCATCTAGCG GGACAACAGT GACTTCTGTG GTGGGGTTTT CCTGTGATGG AATGAGACCT 
    GAGGCCATGA GGCAAGATCC TACGCGGAAG GGCTCAGTGG TCAATGTCAA TCCCACCAAT 
    ACTCGGCCCC AGAGCGACAC CCCAGAGATT CGCAAATACA AGAAGAGGTT TAACTCCGAG 
    ATCCTGTGCG CAGCCTTGTG GGGAGTGAAC TTGTTGGTGG GTACCGAGAG TGGCCTGATG 
    CTGCTGGACA GAAGTGGCCA AGGGAAGGTG TATCCTCTGA TCAACAGAAG GCGATTTCAG 
    CAGATGGACG TCCTGGAAGG CTTGAACGTC TTAGTGACGA TATCTGGCAA AAAGGATAAG 
    TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAACGA CCCGGAGGTT 
    GAGAAGAAGC AGGGCTGGAC GACAGTGGGG GACCTGGAAG GCTGTGTGCA TTATAAAGTT 
    GTAAAATATG AAAGGATCAA ATTTCTGGTC ATTGCTTTGA AGAACTCTGT GGAAGTCTAC 
    GCATGGGCAC CCAAGCCATA TCACAAATTC ATGGCCTTTA AGTCATTTGG TGAGCTGGTG 
    CACAAGCCGT TGCTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGCTGAA AGTGATCTAT 
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGACTCAG GATCGGTCTA TGACATTTAC 
    CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATCC AATGTAGCAT CAAACCCCAC 
    GCAATCATCA TCCTCCCCAA CACAGATGGC ATGGAGCTCC TGGTGTGCTA TGAAGATGAA 
    GGGGTTTACG TGAACACGTA CGGGAGGATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG 
    ATGCCAACGT CTGTAGCATA TATTCGATCC AATCAGACGA TGGGCTGGGG AGAAAAGGCC 
    ATAGAGATCC GATCTGTGGA AACTGGCCAC TTGGATGGCG TGTTTATGCA TAAAAGGGCT 
    CAAAGGCTGA AGTTCTTGTG TGAACGTAAT GACAAGGTGT TCTTTGCCTC CGTCCGGTCT 
    GGGGGCAGCA GCCAGGTTTA CTTCATGACT CTCGGCAGGA CCTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999350.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855118.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X29, mRNA.
    Price & Availability↑
    CloneID OBa220773
    Clone ID Related Accession (Same CDS sequence) XM_024999350.1
    Accession Version XM_024999350.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT CCCTGTGAGA ACAACATCAC GGTCCCCTGT CCTGTCCCGC 
    CGGGATTCCC CACTGCAGGG TAGTGGGCAG CAGAGTAACC AGGCTGGTCA GAGAAACTCC 
    ACCAGTAGCA TCGAGCCGAG GCTGCTCTGG GAGAGAGTGG AGAAGCTGGC GCCCAGGCCG 
    GGCAGTGGCA GCTCCTCGGG GTCCAGCAAC TCGGGGTCCC AGCCCGGCTC CCACCCAGGG 
    TCTCAGAGTG GCTCCGGGGA GCGCTTCAGA GTGAGATCAT CATCCAAATC TGAAGGCTCG 
    CCATCTCAGC GCCTTGAAAA CGCAGCAAAA AAGCCTGAAG ATAAAAAGGA AGTGTTTAGA 
    CCTCTCAAGC CTGCTGGTGA AGTGGACCTG ACGGCTCTGG CCAAAGAGCT GCGGGCCGTG 
    GAGGATGTGC GGCCGCCGCA CAAGGTGACA GACTACTCCT CCTCCAGCGA GGAGTCGGGG 
    ACAACGGACG AGGAGGACGA CGACGTGGAG CAGGAGGGGG CCGAGGAGCC TACGTCTGGC 
    CCGGAGGACA CCAGAGCTGC ATCATCTCTG AACTTGAGCA ATGGTGAAAC AGAGTCCGTG 
    AAAACCATGG TTGTCCATGA CGACGTGGAG AGTGAACCAG CCCTGACCCC GTCCAAAGAG 
    GGCACGCTGA TCGTCCGCCA GAGTGCAGTT GACCAAAAGC GCGCCAGCCA TCATGAGAGC 
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
    TCCACTTCCT CCACCTCCTC TTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
    CAGACACCCC AGGACAAGCT CACTACTAAT GAGACTCAGT CCGCTAGTAA CACACTCCAG 
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
    CCATCTAGCG GGACAACAGT GACTTCTGTG GTGGGGTTTT CCTGTGATGG AATGAGACCT 
    GAGGCCATGA GGCAAGATCC TACGCGGAAG GGCTCAGTGG TCAATGTCAA TCCCACCAAT 
    ACTCGGCCCC AGAGCGACAC CCCAGAGATT CGCAAATACA AGAAGAGGTT TAACTCCGAG 
    ATCCTGTGCG CAGCCTTGTG GGGAGTGAAC TTGTTGGTGG GTACCGAGAG TGGCCTGATG 
    CTGCTGGACA GAAGTGGCCA AGGGAAGGTG TATCCTCTGA TCAACAGAAG GCGATTTCAG 
    CAGATGGACG TCCTGGAAGG CTTGAACGTC TTAGTGACGA TATCTGGCAA AAAGGATAAG 
    TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAACGA CCCGGAGGTT 
    GAGAAGAAGC AGGGCTGGAC GACAGTGGGG GACCTGGAAG GCTGTGTGCA TTATAAAGTT 
    GTAAAATATG AAAGGATCAA ATTTCTGGTC ATTGCTTTGA AGAACTCTGT GGAAGTCTAC 
    GCATGGGCAC CCAAGCCATA TCACAAATTC ATGGCCTTTA AGTCATTTGG TGAGCTGGTG 
    CACAAGCCGT TGCTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGCTGAA AGTGATCTAT 
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGACTCAG GATCGGTCTA TGACATTTAC 
    CTACCAACAC ACATCCAATG TAGCATCAAA CCCCACGCAA TCATCATCCT CCCCAACACA 
    GATGGCATGG AGCTCCTGGT GTGCTATGAA GATGAAGGGG TTTACGTGAA CACGTACGGG 
    AGGATCACCA AGGACGTGGT TCTGCAGTGG GGAGAGATGC CAACGTCTGT AGCATATATT 
    CGATCCAATC AGACGATGGG CTGGGGAGAA AAGGCCATAG AGATCCGATC TGTGGAAACT 
    GGCCACTTGG ATGGCGTGTT TATGCATAAA AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA 
    CGTAATGACA AGGTGTTCTT TGCCTCCGTC CGGTCTGGGG GCAGCAGCCA GGTTTACTTC 
    ATGACTCTCG GCAGGACCTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855118.1
    CDS121..3756
    Translation

    Target ORF information:

    RefSeq Version XM_024999350.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999350.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT CCCTGTGAGA ACAACATCAC GGTCCCCTGT CCTGTCCCGC 
    CGGGATTCCC CACTGCAGGG TAGTGGGCAG CAGAGTAACC AGGCTGGTCA GAGAAACTCC 
    ACCAGTAGCA TCGAGCCGAG GCTGCTCTGG GAGAGAGTGG AGAAGCTGGC GCCCAGGCCG 
    GGCAGTGGCA GCTCCTCGGG GTCCAGCAAC TCGGGGTCCC AGCCCGGCTC CCACCCAGGG 
    TCTCAGAGTG GCTCCGGGGA GCGCTTCAGA GTGAGATCAT CATCCAAATC TGAAGGCTCG 
    CCATCTCAGC GCCTTGAAAA CGCAGCAAAA AAGCCTGAAG ATAAAAAGGA AGTGTTTAGA 
    CCTCTCAAGC CTGCTGGTGA AGTGGACCTG ACGGCTCTGG CCAAAGAGCT GCGGGCCGTG 
    GAGGATGTGC GGCCGCCGCA CAAGGTGACA GACTACTCCT CCTCCAGCGA GGAGTCGGGG 
    ACAACGGACG AGGAGGACGA CGACGTGGAG CAGGAGGGGG CCGAGGAGCC TACGTCTGGC 
    CCGGAGGACA CCAGAGCTGC ATCATCTCTG AACTTGAGCA ATGGTGAAAC AGAGTCCGTG 
    AAAACCATGG TTGTCCATGA CGACGTGGAG AGTGAACCAG CCCTGACCCC GTCCAAAGAG 
    GGCACGCTGA TCGTCCGCCA GAGTGCAGTT GACCAAAAGC GCGCCAGCCA TCATGAGAGC 
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
    TCCACTTCCT CCACCTCCTC TTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
    CAGACACCCC AGGACAAGCT CACTACTAAT GAGACTCAGT CCGCTAGTAA CACACTCCAG 
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
    CCATCTAGCG GGACAACAGT GACTTCTGTG GTGGGGTTTT CCTGTGATGG AATGAGACCT 
    GAGGCCATGA GGCAAGATCC TACGCGGAAG GGCTCAGTGG TCAATGTCAA TCCCACCAAT 
    ACTCGGCCCC AGAGCGACAC CCCAGAGATT CGCAAATACA AGAAGAGGTT TAACTCCGAG 
    ATCCTGTGCG CAGCCTTGTG GGGAGTGAAC TTGTTGGTGG GTACCGAGAG TGGCCTGATG 
    CTGCTGGACA GAAGTGGCCA AGGGAAGGTG TATCCTCTGA TCAACAGAAG GCGATTTCAG 
    CAGATGGACG TCCTGGAAGG CTTGAACGTC TTAGTGACGA TATCTGGCAA AAAGGATAAG 
    TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAACGA CCCGGAGGTT 
    GAGAAGAAGC AGGGCTGGAC GACAGTGGGG GACCTGGAAG GCTGTGTGCA TTATAAAGTT 
    GTAAAATATG AAAGGATCAA ATTTCTGGTC ATTGCTTTGA AGAACTCTGT GGAAGTCTAC 
    GCATGGGCAC CCAAGCCATA TCACAAATTC ATGGCCTTTA AGTCATTTGG TGAGCTGGTG 
    CACAAGCCGT TGCTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGCTGAA AGTGATCTAT 
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGACTCAG GATCGGTCTA TGACATTTAC 
    CTACCAACAC ACATCCAATG TAGCATCAAA CCCCACGCAA TCATCATCCT CCCCAACACA 
    GATGGCATGG AGCTCCTGGT GTGCTATGAA GATGAAGGGG TTTACGTGAA CACGTACGGG 
    AGGATCACCA AGGACGTGGT TCTGCAGTGG GGAGAGATGC CAACGTCTGT AGCATATATT 
    CGATCCAATC AGACGATGGG CTGGGGAGAA AAGGCCATAG AGATCCGATC TGTGGAAACT 
    GGCCACTTGG ATGGCGTGTT TATGCATAAA AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA 
    CGTAATGACA AGGTGTTCTT TGCCTCCGTC CGGTCTGGGG GCAGCAGCCA GGTTTACTTC 
    ATGACTCTCG GCAGGACCTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999351.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855119.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X30, mRNA.
    Price & Availability↑
    CloneID OBa220774
    Clone ID Related Accession (Same CDS sequence) XM_024999351.1
    Accession Version XM_024999351.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3606bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTGTTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCGAGTG GCAACTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTCCCTGTGA GAACAACATC ACGGTCCCCT GTCCTGTCCC GCCGGGATTC CCCACTGCAG 
    GGTAGTGGGC AGCAGAGTAA CCAGGCTGGT CAGAGAAACT CCACCAGTAG CATCGAGCCG 
    AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG GCGCCCAGGC CGGGCAGTGG CAGCTCCTCG 
    GGGTCCAGCA ACTCGGGGTC CCAGCCCGGC TCCCACCCAG GGTCTCAGAG TGGCTCCGGG 
    GAGCGCTTCA GAGTGAGATC ATCATCCAAA TCTGAAGGCT CGCCATCTCA GCGCCTTGAA 
    AACGCAGCAA AAAAGCCTGA AGATAAAAAG GAAGTGTTTA GACCTCTCAA GCCTGCTGGT 
    GAAGTGGACC TGACGGCTCT GGCCAAAGAG CTGCGGGCCG TGGAGGATGT GCGGCCGCCG 
    CACAAGGTGA CAGACTACTC CTCCTCCAGC GAGGAGTCGG GGACAACGGA CGAGGAGGAC 
    GACGACGTGG AGCAGGAGGG GGCCGAGGAG CCTACGTCTG GCCCGGAGGA CACCAGAGCT 
    GCATCATCTC TGAACTTGAG CAATGGTGAA ACAGAGTCCG TGAAAACCAT GGTTGTCCAT 
    GACGACGTGG AGAGTGAACC AGCCCTGACC CCGTCCAAAG AGGGCACGCT GATCGTCCGC 
    CAGACTCAGT CCGCTAGTAA CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
    TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGCG GGACAACAGT GACTTCTGTG 
    GTGGGGTTTT CCTGTGATGG AATGAGACCT GAGGCCATGA GGCAAGATCC TACGCGGAAG 
    GGCTCAGTGG TCAATGTCAA TCCCACCAAT ACTCGGCCCC AGAGCGACAC CCCAGAGATT 
    CGCAAATACA AGAAGAGGTT TAACTCCGAG ATCCTGTGCG CAGCCTTGTG GGGAGTGAAC 
    TTGTTGGTGG GTACCGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTG 
    TATCCTCTGA TCAACAGAAG GCGATTTCAG CAGATGGACG TCCTGGAAGG CTTGAACGTC 
    TTAGTGACGA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA 
    AATAAAATAC TTCACAACGA CCCGGAGGTT GAGAAGAAGC AGGGCTGGAC GACAGTGGGG 
    GACCTGGAAG GCTGTGTGCA TTATAAAGTT GTAAAATATG AAAGGATCAA ATTTCTGGTC 
    ATTGCTTTGA AGAACTCTGT GGAAGTCTAC GCATGGGCAC CCAAGCCATA TCACAAATTC 
    ATGGCCTTTA AGTCATTTGG TGAGCTGGTG CACAAGCCGT TGCTGGTGGA TCTCACTGTT 
    GAGGAAGGCC AGAGGCTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT 
    GTGGACTCAG GATCGGTCTA TGACATTTAC CTACCAACAC ACATCCAATG TAGCATCAAA 
    CCCCACGCAA TCATCATCCT CCCCAACACA GATGGCATGG AGCTCCTGGT GTGCTATGAA 
    GATGAAGGGG TTTACGTGAA CACGTACGGG AGGATCACCA AGGACGTGGT TCTGCAGTGG 
    GGAGAGATGC CAACGTCTGT AGCATATATT CGATCCAATC AGACGATGGG CTGGGGAGAA 
    AAGGCCATAG AGATCCGATC TGTGGAAACT GGCCACTTGG ATGGCGTGTT TATGCATAAA 
    AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA CGTAATGACA AGGTGTTCTT TGCCTCCGTC 
    CGGTCTGGGG GCAGCAGCCA GGTTTACTTC ATGACTCTCG GCAGGACCTC TCTTCTGAGC 
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855119.1
    CDS121..3726
    Translation

    Target ORF information:

    RefSeq Version XM_024999351.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999351.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTGTTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCGAGTG GCAACTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTCCCTGTGA GAACAACATC ACGGTCCCCT GTCCTGTCCC GCCGGGATTC CCCACTGCAG 
    GGTAGTGGGC AGCAGAGTAA CCAGGCTGGT CAGAGAAACT CCACCAGTAG CATCGAGCCG 
    AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG GCGCCCAGGC CGGGCAGTGG CAGCTCCTCG 
    GGGTCCAGCA ACTCGGGGTC CCAGCCCGGC TCCCACCCAG GGTCTCAGAG TGGCTCCGGG 
    GAGCGCTTCA GAGTGAGATC ATCATCCAAA TCTGAAGGCT CGCCATCTCA GCGCCTTGAA 
    AACGCAGCAA AAAAGCCTGA AGATAAAAAG GAAGTGTTTA GACCTCTCAA GCCTGCTGGT 
    GAAGTGGACC TGACGGCTCT GGCCAAAGAG CTGCGGGCCG TGGAGGATGT GCGGCCGCCG 
    CACAAGGTGA CAGACTACTC CTCCTCCAGC GAGGAGTCGG GGACAACGGA CGAGGAGGAC 
    GACGACGTGG AGCAGGAGGG GGCCGAGGAG CCTACGTCTG GCCCGGAGGA CACCAGAGCT 
    GCATCATCTC TGAACTTGAG CAATGGTGAA ACAGAGTCCG TGAAAACCAT GGTTGTCCAT 
    GACGACGTGG AGAGTGAACC AGCCCTGACC CCGTCCAAAG AGGGCACGCT GATCGTCCGC 
    CAGACTCAGT CCGCTAGTAA CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
    TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGCG GGACAACAGT GACTTCTGTG 
    GTGGGGTTTT CCTGTGATGG AATGAGACCT GAGGCCATGA GGCAAGATCC TACGCGGAAG 
    GGCTCAGTGG TCAATGTCAA TCCCACCAAT ACTCGGCCCC AGAGCGACAC CCCAGAGATT 
    CGCAAATACA AGAAGAGGTT TAACTCCGAG ATCCTGTGCG CAGCCTTGTG GGGAGTGAAC 
    TTGTTGGTGG GTACCGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTG 
    TATCCTCTGA TCAACAGAAG GCGATTTCAG CAGATGGACG TCCTGGAAGG CTTGAACGTC 
    TTAGTGACGA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA 
    AATAAAATAC TTCACAACGA CCCGGAGGTT GAGAAGAAGC AGGGCTGGAC GACAGTGGGG 
    GACCTGGAAG GCTGTGTGCA TTATAAAGTT GTAAAATATG AAAGGATCAA ATTTCTGGTC 
    ATTGCTTTGA AGAACTCTGT GGAAGTCTAC GCATGGGCAC CCAAGCCATA TCACAAATTC 
    ATGGCCTTTA AGTCATTTGG TGAGCTGGTG CACAAGCCGT TGCTGGTGGA TCTCACTGTT 
    GAGGAAGGCC AGAGGCTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT 
    GTGGACTCAG GATCGGTCTA TGACATTTAC CTACCAACAC ACATCCAATG TAGCATCAAA 
    CCCCACGCAA TCATCATCCT CCCCAACACA GATGGCATGG AGCTCCTGGT GTGCTATGAA 
    GATGAAGGGG TTTACGTGAA CACGTACGGG AGGATCACCA AGGACGTGGT TCTGCAGTGG 
    GGAGAGATGC CAACGTCTGT AGCATATATT CGATCCAATC AGACGATGGG CTGGGGAGAA 
    AAGGCCATAG AGATCCGATC TGTGGAAACT GGCCACTTGG ATGGCGTGTT TATGCATAAA 
    AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA CGTAATGACA AGGTGTTCTT TGCCTCCGTC 
    CGGTCTGGGG GCAGCAGCCA GGTTTACTTC ATGACTCTCG GCAGGACCTC TCTTCTGAGC 
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999352.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855120.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X31, mRNA.
    Price & Availability↑
    CloneID OBa220775
    Clone ID Related Accession (Same CDS sequence) XM_024999352.1
    Accession Version XM_024999352.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTGTTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCGAGTG GCAACTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTCCCTGTGA GAACAACATC ACGGTCCCCT GTCCTGTCCC GCCGGGATTC CCCACTGCAG 
    GGTAGTGGGC AGCAGAGTAA CCAGGCTGGT CAGAGAAACT CCACCAGTAG CATCGAGCCG 
    AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG GCGCCCAGGC CGGGCAGTGG CAGCTCCTCG 
    GGGTCCAGCA ACTCGGGGTC CCAGCCCGGC TCCCACCCAG GGTCTCAGAG TGGCTCCGGG 
    GAGCGCTTCA GAGTGAGATC ATCATCCAAA TCTGAAGGCT CGCCATCTCA GCGCCTTGAA 
    AACGCAGCAA AAAAGCCTGA AGATAAAAAG GAAGTGTTTA GACCTCTCAA GCCTGCTGAC 
    CTGACGGCTC TGGCCAAAGA GCTGCGGGCC GTGGAGGATG TGCGGCCGCC GCACAAGGTG 
    ACAGACTACT CCTCCTCCAG CGAGGAGTCG GGGACAACGG ACGAGGAGGA CGACGACGTG 
    GAGCAGGAGG GGGCCGAGGA GCCTACGTCT GGCCCGGAGG ACACCAGAGC TGCATCATCT 
    CTGAACTTGA GCAATGGTGA AACAGAGTCC GTGAAAACCA TGGTTGTCCA TGACGACGTG 
    GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA GAGGGCACGC TGATCGTCCG CCAGACTCAG 
    TCCGCTAGTA ACACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
    CCCAGATTAC TACAGATTTC TCCATCTAGC GGGACAACAG TGACTTCTGT GGTGGGGTTT 
    TCCTGTGATG GAATGAGACC TGAGGCCATG AGGCAAGATC CTACGCGGAA GGGCTCAGTG 
    GTCAATGTCA ATCCCACCAA TACTCGGCCC CAGAGCGACA CCCCAGAGAT TCGCAAATAC 
    AAGAAGAGGT TTAACTCCGA GATCCTGTGC GCAGCCTTGT GGGGAGTGAA CTTGTTGGTG 
    GGTACCGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT GTATCCTCTG 
    ATCAACAGAA GGCGATTTCA GCAGATGGAC GTCCTGGAAG GCTTGAACGT CTTAGTGACG 
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA 
    CTTCACAACG ACCCGGAGGT TGAGAAGAAG CAGGGCTGGA CGACAGTGGG GGACCTGGAA 
    GGCTGTGTGC ATTATAAAGT TGTAAAATAT GAAAGGATCA AATTTCTGGT CATTGCTTTG 
    AAGAACTCTG TGGAAGTCTA CGCATGGGCA CCCAAGCCAT ATCACAAATT CATGGCCTTT 
    AAGTCATTTG GTGAGCTGGT GCACAAGCCG TTGCTGGTGG ATCTCACTGT TGAGGAAGGC 
    CAGAGGCTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGACTCA 
    GGATCGGTCT ATGACATTTA CCTACCAACA CACATCCAAT GTAGCATCAA ACCCCACGCA 
    ATCATCATCC TCCCCAACAC AGATGGCATG GAGCTCCTGG TGTGCTATGA AGATGAAGGG 
    GTTTACGTGA ACACGTACGG GAGGATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG 
    CCAACGTCTG TAGCATATAT TCGATCCAAT CAGACGATGG GCTGGGGAGA AAAGGCCATA 
    GAGATCCGAT CTGTGGAAAC TGGCCACTTG GATGGCGTGT TTATGCATAA AAGGGCTCAA 
    AGGCTGAAGT TCTTGTGTGA ACGTAATGAC AAGGTGTTCT TTGCCTCCGT CCGGTCTGGG 
    GGCAGCAGCC AGGTTTACTT CATGACTCTC GGCAGGACCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855120.1
    CDS121..3717
    Translation

    Target ORF information:

    RefSeq Version XM_024999352.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999352.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTGTTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCGAGTG GCAACTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTCCCTGTGA GAACAACATC ACGGTCCCCT GTCCTGTCCC GCCGGGATTC CCCACTGCAG 
    GGTAGTGGGC AGCAGAGTAA CCAGGCTGGT CAGAGAAACT CCACCAGTAG CATCGAGCCG 
    AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG GCGCCCAGGC CGGGCAGTGG CAGCTCCTCG 
    GGGTCCAGCA ACTCGGGGTC CCAGCCCGGC TCCCACCCAG GGTCTCAGAG TGGCTCCGGG 
    GAGCGCTTCA GAGTGAGATC ATCATCCAAA TCTGAAGGCT CGCCATCTCA GCGCCTTGAA 
    AACGCAGCAA AAAAGCCTGA AGATAAAAAG GAAGTGTTTA GACCTCTCAA GCCTGCTGAC 
    CTGACGGCTC TGGCCAAAGA GCTGCGGGCC GTGGAGGATG TGCGGCCGCC GCACAAGGTG 
    ACAGACTACT CCTCCTCCAG CGAGGAGTCG GGGACAACGG ACGAGGAGGA CGACGACGTG 
    GAGCAGGAGG GGGCCGAGGA GCCTACGTCT GGCCCGGAGG ACACCAGAGC TGCATCATCT 
    CTGAACTTGA GCAATGGTGA AACAGAGTCC GTGAAAACCA TGGTTGTCCA TGACGACGTG 
    GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA GAGGGCACGC TGATCGTCCG CCAGACTCAG 
    TCCGCTAGTA ACACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
    CCCAGATTAC TACAGATTTC TCCATCTAGC GGGACAACAG TGACTTCTGT GGTGGGGTTT 
    TCCTGTGATG GAATGAGACC TGAGGCCATG AGGCAAGATC CTACGCGGAA GGGCTCAGTG 
    GTCAATGTCA ATCCCACCAA TACTCGGCCC CAGAGCGACA CCCCAGAGAT TCGCAAATAC 
    AAGAAGAGGT TTAACTCCGA GATCCTGTGC GCAGCCTTGT GGGGAGTGAA CTTGTTGGTG 
    GGTACCGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT GTATCCTCTG 
    ATCAACAGAA GGCGATTTCA GCAGATGGAC GTCCTGGAAG GCTTGAACGT CTTAGTGACG 
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA 
    CTTCACAACG ACCCGGAGGT TGAGAAGAAG CAGGGCTGGA CGACAGTGGG GGACCTGGAA 
    GGCTGTGTGC ATTATAAAGT TGTAAAATAT GAAAGGATCA AATTTCTGGT CATTGCTTTG 
    AAGAACTCTG TGGAAGTCTA CGCATGGGCA CCCAAGCCAT ATCACAAATT CATGGCCTTT 
    AAGTCATTTG GTGAGCTGGT GCACAAGCCG TTGCTGGTGG ATCTCACTGT TGAGGAAGGC 
    CAGAGGCTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGACTCA 
    GGATCGGTCT ATGACATTTA CCTACCAACA CACATCCAAT GTAGCATCAA ACCCCACGCA 
    ATCATCATCC TCCCCAACAC AGATGGCATG GAGCTCCTGG TGTGCTATGA AGATGAAGGG 
    GTTTACGTGA ACACGTACGG GAGGATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG 
    CCAACGTCTG TAGCATATAT TCGATCCAAT CAGACGATGG GCTGGGGAGA AAAGGCCATA 
    GAGATCCGAT CTGTGGAAAC TGGCCACTTG GATGGCGTGT TTATGCATAA AAGGGCTCAA 
    AGGCTGAAGT TCTTGTGTGA ACGTAATGAC AAGGTGTTCT TTGCCTCCGT CCGGTCTGGG 
    GGCAGCAGCC AGGTTTACTT CATGACTCTC GGCAGGACCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_024999353.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855121.1 Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X32, mRNA.
    Price & Availability↑
    CloneID OBa220776
    Clone ID Related Accession (Same CDS sequence) XM_024999353.1
    Accession Version XM_024999353.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT CCCTGTGAGA ACAACATCAC GGTCCCCTGT CCTGTCCCGC 
    CGGGATTCCC CACTGCAGGG TAGTGGGCAG CAGAGTAACC AGGCTGGTCA GAGAAACTCC 
    ACCAGCATCG AGCCGAGGCT GCTCTGGGAG AGAGTGGAGA AGCTGGCGCC CAGGCCGGGC 
    AGTGGCAGCT CCTCGGGGTC CAGCAACTCG GGGTCCCAGC CCGGCTCCCA CCCAGGGTCT 
    CAGAGTGGCT CCGGGGAGCG CTTCAGAGTG AGATCATCAT CCAAATCTGA AGGCTCGCCA 
    TCTCAGCGCC TTGAAAACGC AGCAAAAAAG CCTGAAGATA AAAAGGAAGT GTTTAGACCT 
    CTCAAGCCTG CTGGTGAAGT GGACCTGACG GCTCTGGCCA AAGAGCTGCG GGCCGTGGAG 
    GATGTGCGGC CGCCGCACAA GGTGACAGAC TACTCCTCCT CCAGCGAGGA GTCGGGGACA 
    ACGGACGAGG AGGACGACGA CGTGGAGCAG GAGGGGGCCG AGGAGCCTAC GTCTGGCCCG 
    GAGGACACCA GAGCTGCATC ATCTCTGAAC TTGAGCAATG GTGAAACAGA GTCCGTGAAA 
    ACCATGGTTG TCCATGACGA CGTGGAGAGT GAACCAGCCC TGACCCCGTC CAAAGAGGGC 
    ACGCTGATCG TCCGCCAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC 
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGGACA 
    ACAGTGACTT CTGTGGTGGG GTTTTCCTGT GATGGAATGA GACCTGAGGC CATGAGGCAA 
    GATCCTACGC GGAAGGGCTC AGTGGTCAAT GTCAATCCCA CCAATACTCG GCCCCAGAGC 
    GACACCCCAG AGATTCGCAA ATACAAGAAG AGGTTTAACT CCGAGATCCT GTGCGCAGCC 
    TTGTGGGGAG TGAACTTGTT GGTGGGTACC GAGAGTGGCC TGATGCTGCT GGACAGAAGT 
    GGCCAAGGGA AGGTGTATCC TCTGATCAAC AGAAGGCGAT TTCAGCAGAT GGACGTCCTG 
    GAAGGCTTGA ACGTCTTAGT GACGATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT 
    TTGTCCTGGT TAAGAAATAA AATACTTCAC AACGACCCGG AGGTTGAGAA GAAGCAGGGC 
    TGGACGACAG TGGGGGACCT GGAAGGCTGT GTGCATTATA AAGTTGTAAA ATATGAAAGG 
    ATCAAATTTC TGGTCATTGC TTTGAAGAAC TCTGTGGAAG TCTACGCATG GGCACCCAAG 
    CCATATCACA AATTCATGGC CTTTAAGTCA TTTGGTGAGC TGGTGCACAA GCCGTTGCTG 
    GTGGATCTCA CTGTTGAGGA AGGCCAGAGG CTGAAAGTGA TCTATGGATC CTGTGCTGGA 
    TTCCATGCTG TTGATGTGGA CTCAGGATCG GTCTATGACA TTTACCTACC AACACACGTA 
    AGAAAGAACC CACACTCTAT GATCCAATGT AGCATCAAAC CCCACGCAAT CATCATCCTC 
    CCCAACACAG ATGGCATGGA GCTCCTGGTG TGCTATGAAG ATGAAGGGGT TTACGTGAAC 
    ACGTACGGGA GGATCACCAA GGACGTGGTT CTGCAGTGGG GAGAGATGCC AACGTCTGTA 
    GCATATATTC GATCCAATCA GACGATGGGC TGGGGAGAAA AGGCCATAGA GATCCGATCT 
    GTGGAAACTG GCCACTTGGA TGGCGTGTTT ATGCATAAAA GGGCTCAAAG GCTGAAGTTC 
    TTGTGTGAAC GTAATGACAA GGTGTTCTTT GCCTCCGTCC GGTCTGGGGG CAGCAGCCAG 
    GTTTACTTCA TGACTCTCGG CAGGACCTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855121.1
    CDS121..3585
    Translation

    Target ORF information:

    RefSeq Version XM_024999353.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024999353.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATCG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CGGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTTCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCGTACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCCGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGGGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC TGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGCGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAGTA TATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGTGAGGT CCCTGTGAGA ACAACATCAC GGTCCCCTGT CCTGTCCCGC 
    CGGGATTCCC CACTGCAGGG TAGTGGGCAG CAGAGTAACC AGGCTGGTCA GAGAAACTCC 
    ACCAGCATCG AGCCGAGGCT GCTCTGGGAG AGAGTGGAGA AGCTGGCGCC CAGGCCGGGC 
    AGTGGCAGCT CCTCGGGGTC CAGCAACTCG GGGTCCCAGC CCGGCTCCCA CCCAGGGTCT 
    CAGAGTGGCT CCGGGGAGCG CTTCAGAGTG AGATCATCAT CCAAATCTGA AGGCTCGCCA 
    TCTCAGCGCC TTGAAAACGC AGCAAAAAAG CCTGAAGATA AAAAGGAAGT GTTTAGACCT 
    CTCAAGCCTG CTGGTGAAGT GGACCTGACG GCTCTGGCCA AAGAGCTGCG GGCCGTGGAG 
    GATGTGCGGC CGCCGCACAA GGTGACAGAC TACTCCTCCT CCAGCGAGGA GTCGGGGACA 
    ACGGACGAGG AGGACGACGA CGTGGAGCAG GAGGGGGCCG AGGAGCCTAC GTCTGGCCCG 
    GAGGACACCA GAGCTGCATC ATCTCTGAAC TTGAGCAATG GTGAAACAGA GTCCGTGAAA 
    ACCATGGTTG TCCATGACGA CGTGGAGAGT GAACCAGCCC TGACCCCGTC CAAAGAGGGC 
    ACGCTGATCG TCCGCCAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC 
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGGACA 
    ACAGTGACTT CTGTGGTGGG GTTTTCCTGT GATGGAATGA GACCTGAGGC CATGAGGCAA 
    GATCCTACGC GGAAGGGCTC AGTGGTCAAT GTCAATCCCA CCAATACTCG GCCCCAGAGC 
    GACACCCCAG AGATTCGCAA ATACAAGAAG AGGTTTAACT CCGAGATCCT GTGCGCAGCC 
    TTGTGGGGAG TGAACTTGTT GGTGGGTACC GAGAGTGGCC TGATGCTGCT GGACAGAAGT 
    GGCCAAGGGA AGGTGTATCC TCTGATCAAC AGAAGGCGAT TTCAGCAGAT GGACGTCCTG 
    GAAGGCTTGA ACGTCTTAGT GACGATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT 
    TTGTCCTGGT TAAGAAATAA AATACTTCAC AACGACCCGG AGGTTGAGAA GAAGCAGGGC 
    TGGACGACAG TGGGGGACCT GGAAGGCTGT GTGCATTATA AAGTTGTAAA ATATGAAAGG 
    ATCAAATTTC TGGTCATTGC TTTGAAGAAC TCTGTGGAAG TCTACGCATG GGCACCCAAG 
    CCATATCACA AATTCATGGC CTTTAAGTCA TTTGGTGAGC TGGTGCACAA GCCGTTGCTG 
    GTGGATCTCA CTGTTGAGGA AGGCCAGAGG CTGAAAGTGA TCTATGGATC CTGTGCTGGA 
    TTCCATGCTG TTGATGTGGA CTCAGGATCG GTCTATGACA TTTACCTACC AACACACGTA 
    AGAAAGAACC CACACTCTAT GATCCAATGT AGCATCAAAC CCCACGCAAT CATCATCCTC 
    CCCAACACAG ATGGCATGGA GCTCCTGGTG TGCTATGAAG ATGAAGGGGT TTACGTGAAC 
    ACGTACGGGA GGATCACCAA GGACGTGGTT CTGCAGTGGG GAGAGATGCC AACGTCTGTA 
    GCATATATTC GATCCAATCA GACGATGGGC TGGGGAGAAA AGGCCATAGA GATCCGATCT 
    GTGGAAACTG GCCACTTGGA TGGCGTGTTT ATGCATAAAA GGGCTCAAAG GCTGAAGTTC 
    TTGTGTGAAC GTAATGACAA GGTGTTCTTT GCCTCCGTCC GGTCTGGGGG CAGCAGCCAG 
    GTTTACTTCA TGACTCTCGG CAGGACCTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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