Map4k4 cDNA ORF clone, Mus musculus(house mouse)

The following Map4k4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map4k4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu27453 XM_006496041.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu27454 XM_006496042.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu27455 XM_006496043.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu27456 XM_006496044.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu27457 XM_006496045.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu27458 XM_006496046.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OMu69246 XM_011238530.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu77152 XM_017320994.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu77153 XM_017320998.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu77154 XM_017321001.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu77155 XM_017321002.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu77156 XM_017321004.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu77157 XM_017321010.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu77158 XM_017321011.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu77159 XM_017321013.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu77160 XM_017321016.1
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu77161 XM_017321018.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu77162 XM_017321021.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu85180 XM_030254710.1
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu85181 XM_030254720.1
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu15846 NM_001252200.1
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu15949 NM_008696.2
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu16105 NM_001252202.1
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu16201 NM_001252201.1
Latest version!
Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
View Old Accession Versions >>
OMu77154 XM_017321001.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu27455 XM_006496043.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu77162 XM_017321021.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu27454 XM_006496042.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu69246 XM_011238530.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu77157 XM_017321010.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu77152 XM_017320994.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu77155 XM_017321002.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu77158 XM_017321011.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu77156 XM_017321004.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu77161 XM_017321018.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu27458 XM_006496046.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OMu77153 XM_017320998.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu27453 XM_006496041.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu77159 XM_017321013.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OMu27456 XM_006496044.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OMu27457 XM_006496045.1 Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu27453
    Clone ID Related Accession (Same CDS sequence) XM_006496041.1 , XM_006496041.2
    Accession Version XM_006496041.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496041.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GCAGTATTGA GCCCCGGCTT
    CTTTGGGAGA GAGTGGAAAA GCTGGTCCCC AGGCCAGGCA GTGGCAGCTC TTCAGGGTCC
    AGCAACTCAG GATCCCAGCC TGGCTCCCAT CCTGGGTCTC AGAGCGGCTC CGGTGAACGC
    TTCAGAGTGA GATCATCATC CAAGTCTGAA GGTTCTCCAT CGCCACGCCA GGAAAGTGCA
    GCCAAAAAGC CTGACGATAA GAAAGAAGTA TTCCGGCCTC TCAAGCCTGC GGGAGAAGTG
    GACTTGACTG CGTTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTTCGGCC ACCTCACAAA
    GTAACAGACT ACTCATCCTC CAGTGAGGAG TCTGGGACCA CAGATGAGGA GGAGGAGGAC
    GTGGAACAGG AGGGGGCTGA TGATTCTACC TCGGGACCAG AAGACACCAG AGCAGCGTCA
    TCTCCGAACC TGAGCAACGG TGAAACAGAA TCTGTGAAAA CAATGATCGT TCATGATGAT
    GTAGAAAGTG AGCCAGCCAT GACCCCGTCC AAGGAGGGCA CCCTCATCGT CCGCCAGAGT
    ACAGTTGACC AAAAGCGTGC CAGCCATCAT GAGAGCAATG GCTTTGCGGG TCGCATTCAC
    CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCTTCCTCC
    CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTCACT
    GCTAATGAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT
    ACACCTTTTA TAGACCCCAG GTTACTACAG ATCTCTCCAT CTAGTGGGAC GACAGTGACT
    TCCGTGGTGG GATTTTCCTG TGATGGGCTG AGACCAGAAG CCATAAGGCA AGATCCTACT
    CGGAAAGGCT CAGTGGTCAA TGTGAACCCC ACGAACACTA GGCCACAGAG TGATACCCCC
    GAGATTCGTA AATACAAGAA GAGATTTAAC TCAGAGATCC TGTGTGCTGC CTTATGGGGA
    GTGAACTTGC TGGTTGGTAC AGAGAGCGGT CTGATGCTCT TGGACAGAAG TGGCCAAGGG
    AAAGTATACC CTCTGATCAG CCGAAGACGG TTCCAGCAAA TGGATGTGCT CGAAGGCCTA
    AATGTCCTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTATCCTGG
    CTAAGAAATA AGATTCTTCA TAATGATCCA GAGGTTGAGA AGAAGCAGGG GTGGACCACT
    GTGGGCGACT TGGAAGGGTG CGTGCACTAT AAAGTCGTAA AATATGAAAG AATCAAGTTT
    CTGGTAATTG CTTTGAAGAG TTCTGTGGAA GTCTATGCAT GGGCACCGAA GCCATATCAC
    AAATTTATGG CCTTTAAGTC ATTTGGAGAA CTGCTACATA AGCCATTACT GGTGGATCTC
    ACTGTGGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCGGG ATTCCATGCT
    GTTGATGTGG ATTCAGGCTC AGTCTATGAC ATTTATCTAC CAACACATAT TCAGTGTAGC
    ATCAAACCCC ATGCAATCAT TATCCTCCCC AACACGGATG GGATGGAGCT GCTGGTGTGC
    TACGAAGACG AGGGGGTTTA CGTTAACACG TACGGAAGGA TCACCAAGGA CGTGGTTCTG
    CAGTGGGGAG AGATGCCGAC ATCTGTAGCA TATATCCGGT CGAATCAGAC AATGGGCTGG
    GGAGAGAAGG CAATAGAGAT ACGATCTGTG GAAACTGGTC ACTTGGATGG TGTATTTATG
    CACAAAAGGG CTCAGAGACT AAAGTTCCTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC
    TCCGTCCGCT CTGGTGGCAG CAGCCAGGTC TATTTCATGA CCTTAGGCAG GACTTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496104.1
    CDS329..4480
    Translation

    Target ORF information:

    RefSeq Version XM_006496041.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496041.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GCAGTATTGA GCCCCGGCTT
    CTTTGGGAGA GAGTGGAAAA GCTGGTCCCC AGGCCAGGCA GTGGCAGCTC TTCAGGGTCC
    AGCAACTCAG GATCCCAGCC TGGCTCCCAT CCTGGGTCTC AGAGCGGCTC CGGTGAACGC
    TTCAGAGTGA GATCATCATC CAAGTCTGAA GGTTCTCCAT CGCCACGCCA GGAAAGTGCA
    GCCAAAAAGC CTGACGATAA GAAAGAAGTA TTCCGGCCTC TCAAGCCTGC GGGAGAAGTG
    GACTTGACTG CGTTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTTCGGCC ACCTCACAAA
    GTAACAGACT ACTCATCCTC CAGTGAGGAG TCTGGGACCA CAGATGAGGA GGAGGAGGAC
    GTGGAACAGG AGGGGGCTGA TGATTCTACC TCGGGACCAG AAGACACCAG AGCAGCGTCA
    TCTCCGAACC TGAGCAACGG TGAAACAGAA TCTGTGAAAA CAATGATCGT TCATGATGAT
    GTAGAAAGTG AGCCAGCCAT GACCCCGTCC AAGGAGGGCA CCCTCATCGT CCGCCAGAGT
    ACAGTTGACC AAAAGCGTGC CAGCCATCAT GAGAGCAATG GCTTTGCGGG TCGCATTCAC
    CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCTTCCTCC
    CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTCACT
    GCTAATGAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT
    ACACCTTTTA TAGACCCCAG GTTACTACAG ATCTCTCCAT CTAGTGGGAC GACAGTGACT
    TCCGTGGTGG GATTTTCCTG TGATGGGCTG AGACCAGAAG CCATAAGGCA AGATCCTACT
    CGGAAAGGCT CAGTGGTCAA TGTGAACCCC ACGAACACTA GGCCACAGAG TGATACCCCC
    GAGATTCGTA AATACAAGAA GAGATTTAAC TCAGAGATCC TGTGTGCTGC CTTATGGGGA
    GTGAACTTGC TGGTTGGTAC AGAGAGCGGT CTGATGCTCT TGGACAGAAG TGGCCAAGGG
    AAAGTATACC CTCTGATCAG CCGAAGACGG TTCCAGCAAA TGGATGTGCT CGAAGGCCTA
    AATGTCCTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTATCCTGG
    CTAAGAAATA AGATTCTTCA TAATGATCCA GAGGTTGAGA AGAAGCAGGG GTGGACCACT
    GTGGGCGACT TGGAAGGGTG CGTGCACTAT AAAGTCGTAA AATATGAAAG AATCAAGTTT
    CTGGTAATTG CTTTGAAGAG TTCTGTGGAA GTCTATGCAT GGGCACCGAA GCCATATCAC
    AAATTTATGG CCTTTAAGTC ATTTGGAGAA CTGCTACATA AGCCATTACT GGTGGATCTC
    ACTGTGGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCGGG ATTCCATGCT
    GTTGATGTGG ATTCAGGCTC AGTCTATGAC ATTTATCTAC CAACACATAT TCAGTGTAGC
    ATCAAACCCC ATGCAATCAT TATCCTCCCC AACACGGATG GGATGGAGCT GCTGGTGTGC
    TACGAAGACG AGGGGGTTTA CGTTAACACG TACGGAAGGA TCACCAAGGA CGTGGTTCTG
    CAGTGGGGAG AGATGCCGAC ATCTGTAGCA TATATCCGGT CGAATCAGAC AATGGGCTGG
    GGAGAGAAGG CAATAGAGAT ACGATCTGTG GAAACTGGTC ACTTGGATGG TGTATTTATG
    CACAAAAGGG CTCAGAGACT AAAGTTCCTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC
    TCCGTCCGCT CTGGTGGCAG CAGCCAGGTC TATTTCATGA CCTTAGGCAG GACTTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27454
    Clone ID Related Accession (Same CDS sequence) XM_006496042.1 , XM_006496042.2
    Accession Version XM_006496042.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTACAGTGG TCCCACCTGG CATCTCTCAA GAACAATGTC
    TCCCCTGTCT CGAGATCCCA TTCCTTCAGT GACCCTTCTC CCAAATTCGC ACACCACCAT
    CTCCGCTCTC AGGACCCATG TCCACCTTCC CGCAGTGAGG GGCTCAGTCA GAGCTCTGAC
    TCTAAGTCGG AGGTGCCCGA GCCCACCCAA AAGGCTTGGT CTAGATCAGA CAGTGACGAG
    GTGCCTCCAA GGGTTCCCGT GAGAACGACC TCTCGTTCCC CTGTCCTCTC CCGTCGGGAT
    TCCCCACTGC AGGGCGGTGG ACAGCAGAAT AGCCAAGCAG GACAGAGAAA TTCCACCAGC
    AGTATTGAGC CCCGGCTTCT TTGGGAGAGA GTGGAAAAGC TGGTCCCCAG GCCAGGCAGT
    GGCAGCTCTT CAGGGTCCAG CAACTCAGGA TCCCAGCCTG GCTCCCATCC TGGGTCTCAG
    AGCGGCTCCG GTGAACGCTT CAGAGTGAGA TCATCATCCA AGTCTGAAGG TTCTCCATCG
    CCACGCCAGG AAAGTGCAGC CAAAAAGCCT GACGATAAGA AAGAAGTATT CCGGCCTCTC
    AAGCCTGCGG GAGAAGTGGA CTTGACTGCG TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTTCGGCCAC CTCACAAAGT AACAGACTAC TCATCCTCCA GTGAGGAGTC TGGGACCACA
    GATGAGGAGG AGGAGGACGT GGAACAGGAG GGGGCTGATG ATTCTACCTC GGGACCAGAA
    GACACCAGAG CAGCGTCATC TCCGAACCTG AGCAACGGTG AAACAGAATC TGTGAAAACA
    ATGATCGTTC ATGATGATGT AGAAAGTGAG CCAGCCATGA CCCCGTCCAA GGAGGGCACC
    CTCATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC
    TTTGCGGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CTTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGGT TACTACAGAT CTCTCCATCT
    AGTGGGACGA CAGTGACTTC CGTGGTGGGA TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACTCG GAAAGGCTCA GTGGTCAATG TGAACCCCAC GAACACTAGG
    CCACAGAGTG ATACCCCCGA GATTCGTAAA TACAAGAAGA GATTTAACTC AGAGATCCTG
    TGTGCTGCCT TATGGGGAGT GAACTTGCTG GTTGGTACAG AGAGCGGTCT GATGCTCTTG
    GACAGAAGTG GCCAAGGGAA AGTATACCCT CTGATCAGCC GAAGACGGTT CCAGCAAATG
    GATGTGCTCG AAGGCCTAAA TGTCCTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TATCCTGGCT AAGAAATAAG ATTCTTCATA ATGATCCAGA GGTTGAGAAG
    AAGCAGGGGT GGACCACTGT GGGCGACTTG GAAGGGTGCG TGCACTATAA AGTCGTAAAA
    TATGAAAGAA TCAAGTTTCT GGTAATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCATGG
    GCACCGAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAACT GCTACATAAG
    CCATTACTGG TGGATCTCAC TGTGGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCGGGAT TCCATGCTGT TGATGTGGAT TCAGGCTCAG TCTATGACAT TTATCTACCA
    ACACATATTC AGTGTAGCAT CAAACCCCAT GCAATCATTA TCCTCCCCAA CACGGATGGG
    ATGGAGCTGC TGGTGTGCTA CGAAGACGAG GGGGTTTACG TTAACACGTA CGGAAGGATC
    ACCAAGGACG TGGTTCTGCA GTGGGGAGAG ATGCCGACAT CTGTAGCATA TATCCGGTCG
    AATCAGACAA TGGGCTGGGG AGAGAAGGCA ATAGAGATAC GATCTGTGGA AACTGGTCAC
    TTGGATGGTG TATTTATGCA CAAAAGGGCT CAGAGACTAA AGTTCCTGTG TGAACGCAAT
    GACAAGGTGT TCTTTGCCTC CGTCCGCTCT GGTGGCAGCA GCCAGGTCTA TTTCATGACC
    TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496105.1
    CDS329..4318
    Translation

    Target ORF information:

    RefSeq Version XM_006496042.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496042.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTACAGTGG TCCCACCTGG CATCTCTCAA GAACAATGTC
    TCCCCTGTCT CGAGATCCCA TTCCTTCAGT GACCCTTCTC CCAAATTCGC ACACCACCAT
    CTCCGCTCTC AGGACCCATG TCCACCTTCC CGCAGTGAGG GGCTCAGTCA GAGCTCTGAC
    TCTAAGTCGG AGGTGCCCGA GCCCACCCAA AAGGCTTGGT CTAGATCAGA CAGTGACGAG
    GTGCCTCCAA GGGTTCCCGT GAGAACGACC TCTCGTTCCC CTGTCCTCTC CCGTCGGGAT
    TCCCCACTGC AGGGCGGTGG ACAGCAGAAT AGCCAAGCAG GACAGAGAAA TTCCACCAGC
    AGTATTGAGC CCCGGCTTCT TTGGGAGAGA GTGGAAAAGC TGGTCCCCAG GCCAGGCAGT
    GGCAGCTCTT CAGGGTCCAG CAACTCAGGA TCCCAGCCTG GCTCCCATCC TGGGTCTCAG
    AGCGGCTCCG GTGAACGCTT CAGAGTGAGA TCATCATCCA AGTCTGAAGG TTCTCCATCG
    CCACGCCAGG AAAGTGCAGC CAAAAAGCCT GACGATAAGA AAGAAGTATT CCGGCCTCTC
    AAGCCTGCGG GAGAAGTGGA CTTGACTGCG TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTTCGGCCAC CTCACAAAGT AACAGACTAC TCATCCTCCA GTGAGGAGTC TGGGACCACA
    GATGAGGAGG AGGAGGACGT GGAACAGGAG GGGGCTGATG ATTCTACCTC GGGACCAGAA
    GACACCAGAG CAGCGTCATC TCCGAACCTG AGCAACGGTG AAACAGAATC TGTGAAAACA
    ATGATCGTTC ATGATGATGT AGAAAGTGAG CCAGCCATGA CCCCGTCCAA GGAGGGCACC
    CTCATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC
    TTTGCGGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CTTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGGT TACTACAGAT CTCTCCATCT
    AGTGGGACGA CAGTGACTTC CGTGGTGGGA TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACTCG GAAAGGCTCA GTGGTCAATG TGAACCCCAC GAACACTAGG
    CCACAGAGTG ATACCCCCGA GATTCGTAAA TACAAGAAGA GATTTAACTC AGAGATCCTG
    TGTGCTGCCT TATGGGGAGT GAACTTGCTG GTTGGTACAG AGAGCGGTCT GATGCTCTTG
    GACAGAAGTG GCCAAGGGAA AGTATACCCT CTGATCAGCC GAAGACGGTT CCAGCAAATG
    GATGTGCTCG AAGGCCTAAA TGTCCTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TATCCTGGCT AAGAAATAAG ATTCTTCATA ATGATCCAGA GGTTGAGAAG
    AAGCAGGGGT GGACCACTGT GGGCGACTTG GAAGGGTGCG TGCACTATAA AGTCGTAAAA
    TATGAAAGAA TCAAGTTTCT GGTAATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCATGG
    GCACCGAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAACT GCTACATAAG
    CCATTACTGG TGGATCTCAC TGTGGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCGGGAT TCCATGCTGT TGATGTGGAT TCAGGCTCAG TCTATGACAT TTATCTACCA
    ACACATATTC AGTGTAGCAT CAAACCCCAT GCAATCATTA TCCTCCCCAA CACGGATGGG
    ATGGAGCTGC TGGTGTGCTA CGAAGACGAG GGGGTTTACG TTAACACGTA CGGAAGGATC
    ACCAAGGACG TGGTTCTGCA GTGGGGAGAG ATGCCGACAT CTGTAGCATA TATCCGGTCG
    AATCAGACAA TGGGCTGGGG AGAGAAGGCA ATAGAGATAC GATCTGTGGA AACTGGTCAC
    TTGGATGGTG TATTTATGCA CAAAAGGGCT CAGAGACTAA AGTTCCTGTG TGAACGCAAT
    GACAAGGTGT TCTTTGCCTC CGTCCGCTCT GGTGGCAGCA GCCAGGTCTA TTTCATGACC
    TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27455
    Clone ID Related Accession (Same CDS sequence) XM_006496043.1 , XM_006496043.2
    Accession Version XM_006496043.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496043.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTTCCCGTGA GAACGACCTC TCGTTCCCCT
    GTCCTCTCCC GTCGGGATTC CCCACTGCAG GGCGGTGGAC AGCAGAATAG CCAAGCAGGA
    CAGAGAAATT CCACCAGCAG TATTGAGCCC CGGCTTCTTT GGGAGAGAGT GGAAAAGCTG
    GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA GGGTCCAGCA ACTCAGGATC CCAGCCTGGC
    TCCCATCCTG GGTCTCAGAG CGGCTCCGGT GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGTT CTCCATCGCC ACGCCAGGAA AGTGCAGCCA AAAAGCCTGA CGATAAGAAA
    GAAGTATTCC GGCCTCTCAA GCCTGCGGAC TTGACTGCGT TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TTCGGCCACC TCACAAAGTA ACAGACTACT CATCCTCCAG TGAGGAGTCT
    GGGACCACAG ATGAGGAGGA GGAGGACGTG GAACAGGAGG GGGCTGATGA TTCTACCTCG
    GGACCAGAAG ACACCAGAGC AGCGTCATCT CCGAACCTGA GCAACGGTGA AACAGAATCT
    GTGAAAACAA TGATCGTTCA TGATGATGTA GAAAGTGAGC CAGCCATGAC CCCGTCCAAG
    GAGGGCACCC TCATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCGGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC TTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGGTT ACTACAGATC
    TCTCCATCTA GTGGGACGAC AGTGACTTCC GTGGTGGGAT TTTCCTGTGA TGGGCTGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACTCGG AAAGGCTCAG TGGTCAATGT GAACCCCACG
    AACACTAGGC CACAGAGTGA TACCCCCGAG ATTCGTAAAT ACAAGAAGAG ATTTAACTCA
    GAGATCCTGT GTGCTGCCTT ATGGGGAGTG AACTTGCTGG TTGGTACAGA GAGCGGTCTG
    ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA GTATACCCTC TGATCAGCCG AAGACGGTTC
    CAGCAAATGG ATGTGCTCGA AGGCCTAAAT GTCCTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT ATCCTGGCTA AGAAATAAGA TTCTTCATAA TGATCCAGAG
    GTTGAGAAGA AGCAGGGGTG GACCACTGTG GGCGACTTGG AAGGGTGCGT GCACTATAAA
    GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG GTAATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCATGGG CACCGAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAACTG
    CTACATAAGC CATTACTGGT GGATCTCACT GTGGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCGGGATT CCATGCTGTT GATGTGGATT CAGGCTCAGT CTATGACATT
    TATCTACCAA CACATATTCA GTGTAGCATC AAACCCCATG CAATCATTAT CCTCCCCAAC
    ACGGATGGGA TGGAGCTGCT GGTGTGCTAC GAAGACGAGG GGGTTTACGT TAACACGTAC
    GGAAGGATCA CCAAGGACGT GGTTCTGCAG TGGGGAGAGA TGCCGACATC TGTAGCATAT
    ATCCGGTCGA ATCAGACAAT GGGCTGGGGA GAGAAGGCAA TAGAGATACG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT ATTTATGCAC AAAAGGGCTC AGAGACTAAA GTTCCTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCC GTCCGCTCTG GTGGCAGCAG CCAGGTCTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496106.1
    CDS329..4147
    Translation

    Target ORF information:

    RefSeq Version XM_006496043.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496043.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTTCCCGTGA GAACGACCTC TCGTTCCCCT
    GTCCTCTCCC GTCGGGATTC CCCACTGCAG GGCGGTGGAC AGCAGAATAG CCAAGCAGGA
    CAGAGAAATT CCACCAGCAG TATTGAGCCC CGGCTTCTTT GGGAGAGAGT GGAAAAGCTG
    GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA GGGTCCAGCA ACTCAGGATC CCAGCCTGGC
    TCCCATCCTG GGTCTCAGAG CGGCTCCGGT GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGTT CTCCATCGCC ACGCCAGGAA AGTGCAGCCA AAAAGCCTGA CGATAAGAAA
    GAAGTATTCC GGCCTCTCAA GCCTGCGGAC TTGACTGCGT TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TTCGGCCACC TCACAAAGTA ACAGACTACT CATCCTCCAG TGAGGAGTCT
    GGGACCACAG ATGAGGAGGA GGAGGACGTG GAACAGGAGG GGGCTGATGA TTCTACCTCG
    GGACCAGAAG ACACCAGAGC AGCGTCATCT CCGAACCTGA GCAACGGTGA AACAGAATCT
    GTGAAAACAA TGATCGTTCA TGATGATGTA GAAAGTGAGC CAGCCATGAC CCCGTCCAAG
    GAGGGCACCC TCATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCGGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC TTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGGTT ACTACAGATC
    TCTCCATCTA GTGGGACGAC AGTGACTTCC GTGGTGGGAT TTTCCTGTGA TGGGCTGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACTCGG AAAGGCTCAG TGGTCAATGT GAACCCCACG
    AACACTAGGC CACAGAGTGA TACCCCCGAG ATTCGTAAAT ACAAGAAGAG ATTTAACTCA
    GAGATCCTGT GTGCTGCCTT ATGGGGAGTG AACTTGCTGG TTGGTACAGA GAGCGGTCTG
    ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA GTATACCCTC TGATCAGCCG AAGACGGTTC
    CAGCAAATGG ATGTGCTCGA AGGCCTAAAT GTCCTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT ATCCTGGCTA AGAAATAAGA TTCTTCATAA TGATCCAGAG
    GTTGAGAAGA AGCAGGGGTG GACCACTGTG GGCGACTTGG AAGGGTGCGT GCACTATAAA
    GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG GTAATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCATGGG CACCGAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAACTG
    CTACATAAGC CATTACTGGT GGATCTCACT GTGGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCGGGATT CCATGCTGTT GATGTGGATT CAGGCTCAGT CTATGACATT
    TATCTACCAA CACATATTCA GTGTAGCATC AAACCCCATG CAATCATTAT CCTCCCCAAC
    ACGGATGGGA TGGAGCTGCT GGTGTGCTAC GAAGACGAGG GGGTTTACGT TAACACGTAC
    GGAAGGATCA CCAAGGACGT GGTTCTGCAG TGGGGAGAGA TGCCGACATC TGTAGCATAT
    ATCCGGTCGA ATCAGACAAT GGGCTGGGGA GAGAAGGCAA TAGAGATACG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT ATTTATGCAC AAAAGGGCTC AGAGACTAAA GTTCCTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCC GTCCGCTCTG GTGGCAGCAG CCAGGTCTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27456
    Clone ID Related Accession (Same CDS sequence) XM_006496044.1 , XM_006496044.2
    Accession Version XM_006496044.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496044.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTTCCCG TGAGAACGAC CTCTCGTTCC CCTGTCCTCT CCCGTCGGGA TTCCCCACTG
    CAGGGCGGTG GACAGCAGAA TAGCCAAGCA GGACAGAGAA ATTCCACCAG TATTGAGCCC
    CGGCTTCTTT GGGAGAGAGT GGAAAAGCTG GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA
    GGGTCCAGCA ACTCAGGATC CCAGCCTGGC TCCCATCCTG GGTCTCAGAG CGGCTCCGGT
    GAACGCTTCA GAGTGAGATC ATCATCCAAG TCTGAAGGTT CTCCATCGCC ACGCCAGGAA
    AGTGCAGCCA AAAAGCCTGA CGATAAGAAA GAAGTATTCC GGCCTCTCAA GCCTGCGGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TTCGGCCACC TCACAAAGTA
    ACAGACTACT CATCCTCCAG TGAGGAGTCT GGGACCACAG ATGAGGAGGA GGAGGACGTG
    GAACAGGAGG GGGCTGATGA TTCTACCTCG GGACCAGAAG ACACCAGAGC AGCGTCATCT
    CCGAACCTGA GCAACGGTGA AACAGAATCT GTGAAAACAA TGATCGTTCA TGATGATGTA
    GAAAGTGAGC CAGCCATGAC CCCGTCCAAG GAGGGCACCC TCATCGTCCG CCAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGGTTAC TACAGATCTC TCCATCTAGT GGGACGACAG TGACTTCCGT GGTGGGATTT
    TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG
    GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC
    AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA CTTGCTGGTT
    GGTACAGAGA GCGGTCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT ATACCCTCTG
    ATCAGCCGAA GACGGTTCCA GCAAATGGAT GTGCTCGAAG GCCTAAATGT CCTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTAT CCTGGCTAAG AAATAAGATT
    CTTCATAATG ATCCAGAGGT TGAGAAGAAG CAGGGGTGGA CCACTGTGGG CGACTTGGAA
    GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATTGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAACTGCT ACATAAGCCA TTACTGGTGG ATCTCACTGT GGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCGGGATTCC ATGCTGTTGA TGTGGATTCA
    GGCTCAGTCT ATGACATTTA TCTACCAACA CATATTCAGT GTAGCATCAA ACCCCATGCA
    ATCATTATCC TCCCCAACAC GGATGGGATG GAGCTGCTGG TGTGCTACGA AGACGAGGGG
    GTTTACGTTA ACACGTACGG AAGGATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG
    CCGACATCTG TAGCATATAT CCGGTCGAAT CAGACAATGG GCTGGGGAGA GAAGGCAATA
    GAGATACGAT CTGTGGAAAC TGGTCACTTG GATGGTGTAT TTATGCACAA AAGGGCTCAG
    AGACTAAAGT TCCTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCCGT CCGCTCTGGT
    GGCAGCAGCC AGGTCTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496107.1
    CDS329..4045
    Translation

    Target ORF information:

    RefSeq Version XM_006496044.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496044.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTTCCCG TGAGAACGAC CTCTCGTTCC CCTGTCCTCT CCCGTCGGGA TTCCCCACTG
    CAGGGCGGTG GACAGCAGAA TAGCCAAGCA GGACAGAGAA ATTCCACCAG TATTGAGCCC
    CGGCTTCTTT GGGAGAGAGT GGAAAAGCTG GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA
    GGGTCCAGCA ACTCAGGATC CCAGCCTGGC TCCCATCCTG GGTCTCAGAG CGGCTCCGGT
    GAACGCTTCA GAGTGAGATC ATCATCCAAG TCTGAAGGTT CTCCATCGCC ACGCCAGGAA
    AGTGCAGCCA AAAAGCCTGA CGATAAGAAA GAAGTATTCC GGCCTCTCAA GCCTGCGGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TTCGGCCACC TCACAAAGTA
    ACAGACTACT CATCCTCCAG TGAGGAGTCT GGGACCACAG ATGAGGAGGA GGAGGACGTG
    GAACAGGAGG GGGCTGATGA TTCTACCTCG GGACCAGAAG ACACCAGAGC AGCGTCATCT
    CCGAACCTGA GCAACGGTGA AACAGAATCT GTGAAAACAA TGATCGTTCA TGATGATGTA
    GAAAGTGAGC CAGCCATGAC CCCGTCCAAG GAGGGCACCC TCATCGTCCG CCAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGGTTAC TACAGATCTC TCCATCTAGT GGGACGACAG TGACTTCCGT GGTGGGATTT
    TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG
    GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC
    AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA CTTGCTGGTT
    GGTACAGAGA GCGGTCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT ATACCCTCTG
    ATCAGCCGAA GACGGTTCCA GCAAATGGAT GTGCTCGAAG GCCTAAATGT CCTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTAT CCTGGCTAAG AAATAAGATT
    CTTCATAATG ATCCAGAGGT TGAGAAGAAG CAGGGGTGGA CCACTGTGGG CGACTTGGAA
    GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATTGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAACTGCT ACATAAGCCA TTACTGGTGG ATCTCACTGT GGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCGGGATTCC ATGCTGTTGA TGTGGATTCA
    GGCTCAGTCT ATGACATTTA TCTACCAACA CATATTCAGT GTAGCATCAA ACCCCATGCA
    ATCATTATCC TCCCCAACAC GGATGGGATG GAGCTGCTGG TGTGCTACGA AGACGAGGGG
    GTTTACGTTA ACACGTACGG AAGGATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG
    CCGACATCTG TAGCATATAT CCGGTCGAAT CAGACAATGG GCTGGGGAGA GAAGGCAATA
    GAGATACGAT CTGTGGAAAC TGGTCACTTG GATGGTGTAT TTATGCACAA AAGGGCTCAG
    AGACTAAAGT TCCTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCCGT CCGCTCTGGT
    GGCAGCAGCC AGGTCTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27457
    Clone ID Related Accession (Same CDS sequence) XM_006496045.1 , XM_006496045.2
    Accession Version XM_006496045.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTTCCCGTGA GAACGACCTC TCGTTCCCCT
    GTCCTCTCCC GTCGGGATTC CCCACTGCAG GGCGGTGGAC AGCAGAATAG CCAAGCAGGA
    CAGAGAAATT CCACCAGCAG TATTGAGCCC CGGCTTCTTT GGGAGAGAGT GGAAAAGCTG
    GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA GGGTCCAGCA ACTCAGGATC CCAGCCTGGC
    TCCCATCCTG GGTCTCAGAG CGGCTCCGGT GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGTT CTCCATCGCC ACGCCAGGAA AGTGCAGCCA AAAAGCCTGA CGATAAGAAA
    GAAGTATTCC GGCCTCTCAA GCCTGCGGGA GAAGTGGACT TGACTGCGTT GGCCAAAGAG
    CTTCGAGCAG TGGAAGATGT TCGGCCACCT CACAAAGTAA CAGACTACTC ATCCTCCAGT
    GAGGAGTCTG GGACCACAGA TGAGGAGGAG GAGGACGTGG AACAGGAGGG GGCTGATGAT
    TCTACCTCGG GACCAGAAGA CACCAGAGCA GCGTCATCTC CGAACCTGAG CAACGGTGAA
    ACAGAATCTG TGAAAACAAT GATCGTTCAT GATGATGTAG AAAGTGAGCC AGCCATGACC
    CCGTCCAAGG AGGGCACCCT CATCGTCCGC CAGACTCAGT CCGCTAGTAG CACACTCCAG
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGGTTACT ACAGATCTCT
    CCATCTAGTG GGACGACAGT GACTTCCGTG GTGGGATTTT CCTGTGATGG GCTGAGACCA
    GAAGCCATAA GGCAAGATCC TACTCGGAAA GGCTCAGTGG TCAATGTGAA CCCCACGAAC
    ACTAGGCCAC AGAGTGATAC CCCCGAGATT CGTAAATACA AGAAGAGATT TAACTCAGAG
    ATCCTGTGTG CTGCCTTATG GGGAGTGAAC TTGCTGGTTG GTACAGAGAG CGGTCTGATG
    CTCTTGGACA GAAGTGGCCA AGGGAAAGTA TACCCTCTGA TCAGCCGAAG ACGGTTCCAG
    CAAATGGATG TGCTCGAAGG CCTAAATGTC CTGGTGACAA TATCTGGCAA AAAGGATAAG
    TTACGTGTCT ACTATTTATC CTGGCTAAGA AATAAGATTC TTCATAATGA TCCAGAGGTT
    GAGAAGAAGC AGGGGTGGAC CACTGTGGGC GACTTGGAAG GGTGCGTGCA CTATAAAGTC
    GTAAAATATG AAAGAATCAA GTTTCTGGTA ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT
    GCATGGGCAC CGAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAACTGCTA
    CATAAGCCAT TACTGGTGGA TCTCACTGTG GAGGAAGGCC AGAGGTTGAA AGTGATCTAT
    GGATCCTGTG CGGGATTCCA TGCTGTTGAT GTGGATTCAG GCTCAGTCTA TGACATTTAT
    CTACCAACAC ATATTCAGTG TAGCATCAAA CCCCATGCAA TCATTATCCT CCCCAACACG
    GATGGGATGG AGCTGCTGGT GTGCTACGAA GACGAGGGGG TTTACGTTAA CACGTACGGA
    AGGATCACCA AGGACGTGGT TCTGCAGTGG GGAGAGATGC CGACATCTGT AGCATATATC
    CGGTCGAATC AGACAATGGG CTGGGGAGAG AAGGCAATAG AGATACGATC TGTGGAAACT
    GGTCACTTGG ATGGTGTATT TATGCACAAA AGGGCTCAGA GACTAAAGTT CCTGTGTGAA
    CGCAATGACA AGGTGTTCTT TGCCTCCGTC CGCTCTGGTG GCAGCAGCCA GGTCTATTTC
    ATGACCTTAG GCAGGACTTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496108.1
    CDS329..3964
    Translation

    Target ORF information:

    RefSeq Version XM_006496045.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496045.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTTCCCGTGA GAACGACCTC TCGTTCCCCT
    GTCCTCTCCC GTCGGGATTC CCCACTGCAG GGCGGTGGAC AGCAGAATAG CCAAGCAGGA
    CAGAGAAATT CCACCAGCAG TATTGAGCCC CGGCTTCTTT GGGAGAGAGT GGAAAAGCTG
    GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA GGGTCCAGCA ACTCAGGATC CCAGCCTGGC
    TCCCATCCTG GGTCTCAGAG CGGCTCCGGT GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGTT CTCCATCGCC ACGCCAGGAA AGTGCAGCCA AAAAGCCTGA CGATAAGAAA
    GAAGTATTCC GGCCTCTCAA GCCTGCGGGA GAAGTGGACT TGACTGCGTT GGCCAAAGAG
    CTTCGAGCAG TGGAAGATGT TCGGCCACCT CACAAAGTAA CAGACTACTC ATCCTCCAGT
    GAGGAGTCTG GGACCACAGA TGAGGAGGAG GAGGACGTGG AACAGGAGGG GGCTGATGAT
    TCTACCTCGG GACCAGAAGA CACCAGAGCA GCGTCATCTC CGAACCTGAG CAACGGTGAA
    ACAGAATCTG TGAAAACAAT GATCGTTCAT GATGATGTAG AAAGTGAGCC AGCCATGACC
    CCGTCCAAGG AGGGCACCCT CATCGTCCGC CAGACTCAGT CCGCTAGTAG CACACTCCAG
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGGTTACT ACAGATCTCT
    CCATCTAGTG GGACGACAGT GACTTCCGTG GTGGGATTTT CCTGTGATGG GCTGAGACCA
    GAAGCCATAA GGCAAGATCC TACTCGGAAA GGCTCAGTGG TCAATGTGAA CCCCACGAAC
    ACTAGGCCAC AGAGTGATAC CCCCGAGATT CGTAAATACA AGAAGAGATT TAACTCAGAG
    ATCCTGTGTG CTGCCTTATG GGGAGTGAAC TTGCTGGTTG GTACAGAGAG CGGTCTGATG
    CTCTTGGACA GAAGTGGCCA AGGGAAAGTA TACCCTCTGA TCAGCCGAAG ACGGTTCCAG
    CAAATGGATG TGCTCGAAGG CCTAAATGTC CTGGTGACAA TATCTGGCAA AAAGGATAAG
    TTACGTGTCT ACTATTTATC CTGGCTAAGA AATAAGATTC TTCATAATGA TCCAGAGGTT
    GAGAAGAAGC AGGGGTGGAC CACTGTGGGC GACTTGGAAG GGTGCGTGCA CTATAAAGTC
    GTAAAATATG AAAGAATCAA GTTTCTGGTA ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT
    GCATGGGCAC CGAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAACTGCTA
    CATAAGCCAT TACTGGTGGA TCTCACTGTG GAGGAAGGCC AGAGGTTGAA AGTGATCTAT
    GGATCCTGTG CGGGATTCCA TGCTGTTGAT GTGGATTCAG GCTCAGTCTA TGACATTTAT
    CTACCAACAC ATATTCAGTG TAGCATCAAA CCCCATGCAA TCATTATCCT CCCCAACACG
    GATGGGATGG AGCTGCTGGT GTGCTACGAA GACGAGGGGG TTTACGTTAA CACGTACGGA
    AGGATCACCA AGGACGTGGT TCTGCAGTGG GGAGAGATGC CGACATCTGT AGCATATATC
    CGGTCGAATC AGACAATGGG CTGGGGAGAG AAGGCAATAG AGATACGATC TGTGGAAACT
    GGTCACTTGG ATGGTGTATT TATGCACAAA AGGGCTCAGA GACTAAAGTT CCTGTGTGAA
    CGCAATGACA AGGTGTTCTT TGCCTCCGTC CGCTCTGGTG GCAGCAGCCA GGTCTATTTC
    ATGACCTTAG GCAGGACTTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27458
    Clone ID Related Accession (Same CDS sequence) XM_006496046.1 , XM_006496046.2
    Accession Version XM_006496046.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006496046.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT TCCCGTGAGA
    ACGACCTCTC GTTCCCCTGT CCTCTCCCGT CGGGATTCCC CACTGCAGGG CGGTGGACAG
    CAGAATAGCC AAGCAGGACA GAGAAATTCC ACCAGTATTG AGCCCCGGCT TCTTTGGGAG
    AGAGTGGAAA AGCTGGTCCC CAGGCCAGGC AGTGGCAGCT CTTCAGGGTC CAGCAACTCA
    GGATCCCAGC CTGGCTCCCA TCCTGGGTCT CAGAGCGGCT CCGGTGAACG CTTCAGAGTG
    AGATCATCAT CCAAGTCTGA AGGTTCTCCA TCGCCACGCC AGGAAAGTGC AGCCAAAAAG
    CCTGACGATA AGAAAGAAGT ATTCCGGCCT CTCAAGCCTG CGGGAGAAGT GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTTCGGC CACCTCACAA AGTAACAGAC
    TACTCATCCT CCAGTGAGGA GTCTGGGACC ACAGATGAGG AGGAGGAGGA CGTGGAACAG
    GAGGGGGCTG ATGATTCTAC CTCGGGACCA GAAGACACCA GAGCAGCGTC ATCTCCGAAC
    CTGAGCAACG GTGAAACAGA ATCTGTGAAA ACAATGATCG TTCATGATGA TGTAGAAAGT
    GAGCCAGCCA TGACCCCGTC CAAGGAGGGC ACCCTCATCG TCCGCCAGAC TCAGTCCGCT
    AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGG
    TTACTACAGA TCTCTCCATC TAGTGGGACG ACAGTGACTT CCGTGGTGGG ATTTTCCTGT
    GATGGGCTGA GACCAGAAGC CATAAGGCAA GATCCTACTC GGAAAGGCTC AGTGGTCAAT
    GTGAACCCCA CGAACACTAG GCCACAGAGT GATACCCCCG AGATTCGTAA ATACAAGAAG
    AGATTTAACT CAGAGATCCT GTGTGCTGCC TTATGGGGAG TGAACTTGCT GGTTGGTACA
    GAGAGCGGTC TGATGCTCTT GGACAGAAGT GGCCAAGGGA AAGTATACCC TCTGATCAGC
    CGAAGACGGT TCCAGCAAAT GGATGTGCTC GAAGGCCTAA ATGTCCTGGT GACAATATCT
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTATCCTGGC TAAGAAATAA GATTCTTCAT
    AATGATCCAG AGGTTGAGAA GAAGCAGGGG TGGACCACTG TGGGCGACTT GGAAGGGTGC
    GTGCACTATA AAGTCGTAAA ATATGAAAGA ATCAAGTTTC TGGTAATTGC TTTGAAGAGT
    TCTGTGGAAG TCTATGCATG GGCACCGAAG CCATATCACA AATTTATGGC CTTTAAGTCA
    TTTGGAGAAC TGCTACATAA GCCATTACTG GTGGATCTCA CTGTGGAGGA AGGCCAGAGG
    TTGAAAGTGA TCTATGGATC CTGTGCGGGA TTCCATGCTG TTGATGTGGA TTCAGGCTCA
    GTCTATGACA TTTATCTACC AACACATATT CAGTGTAGCA TCAAACCCCA TGCAATCATT
    ATCCTCCCCA ACACGGATGG GATGGAGCTG CTGGTGTGCT ACGAAGACGA GGGGGTTTAC
    GTTAACACGT ACGGAAGGAT CACCAAGGAC GTGGTTCTGC AGTGGGGAGA GATGCCGACA
    TCTGTAGCAT ATATCCGGTC GAATCAGACA ATGGGCTGGG GAGAGAAGGC AATAGAGATA
    CGATCTGTGG AAACTGGTCA CTTGGATGGT GTATTTATGC ACAAAAGGGC TCAGAGACTA
    AAGTTCCTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CCGTCCGCTC TGGTGGCAGC
    AGCCAGGTCT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496109.1
    CDS329..3799
    Translation

    Target ORF information:

    RefSeq Version XM_006496046.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496046.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT TCCCGTGAGA
    ACGACCTCTC GTTCCCCTGT CCTCTCCCGT CGGGATTCCC CACTGCAGGG CGGTGGACAG
    CAGAATAGCC AAGCAGGACA GAGAAATTCC ACCAGTATTG AGCCCCGGCT TCTTTGGGAG
    AGAGTGGAAA AGCTGGTCCC CAGGCCAGGC AGTGGCAGCT CTTCAGGGTC CAGCAACTCA
    GGATCCCAGC CTGGCTCCCA TCCTGGGTCT CAGAGCGGCT CCGGTGAACG CTTCAGAGTG
    AGATCATCAT CCAAGTCTGA AGGTTCTCCA TCGCCACGCC AGGAAAGTGC AGCCAAAAAG
    CCTGACGATA AGAAAGAAGT ATTCCGGCCT CTCAAGCCTG CGGGAGAAGT GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTTCGGC CACCTCACAA AGTAACAGAC
    TACTCATCCT CCAGTGAGGA GTCTGGGACC ACAGATGAGG AGGAGGAGGA CGTGGAACAG
    GAGGGGGCTG ATGATTCTAC CTCGGGACCA GAAGACACCA GAGCAGCGTC ATCTCCGAAC
    CTGAGCAACG GTGAAACAGA ATCTGTGAAA ACAATGATCG TTCATGATGA TGTAGAAAGT
    GAGCCAGCCA TGACCCCGTC CAAGGAGGGC ACCCTCATCG TCCGCCAGAC TCAGTCCGCT
    AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGG
    TTACTACAGA TCTCTCCATC TAGTGGGACG ACAGTGACTT CCGTGGTGGG ATTTTCCTGT
    GATGGGCTGA GACCAGAAGC CATAAGGCAA GATCCTACTC GGAAAGGCTC AGTGGTCAAT
    GTGAACCCCA CGAACACTAG GCCACAGAGT GATACCCCCG AGATTCGTAA ATACAAGAAG
    AGATTTAACT CAGAGATCCT GTGTGCTGCC TTATGGGGAG TGAACTTGCT GGTTGGTACA
    GAGAGCGGTC TGATGCTCTT GGACAGAAGT GGCCAAGGGA AAGTATACCC TCTGATCAGC
    CGAAGACGGT TCCAGCAAAT GGATGTGCTC GAAGGCCTAA ATGTCCTGGT GACAATATCT
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTATCCTGGC TAAGAAATAA GATTCTTCAT
    AATGATCCAG AGGTTGAGAA GAAGCAGGGG TGGACCACTG TGGGCGACTT GGAAGGGTGC
    GTGCACTATA AAGTCGTAAA ATATGAAAGA ATCAAGTTTC TGGTAATTGC TTTGAAGAGT
    TCTGTGGAAG TCTATGCATG GGCACCGAAG CCATATCACA AATTTATGGC CTTTAAGTCA
    TTTGGAGAAC TGCTACATAA GCCATTACTG GTGGATCTCA CTGTGGAGGA AGGCCAGAGG
    TTGAAAGTGA TCTATGGATC CTGTGCGGGA TTCCATGCTG TTGATGTGGA TTCAGGCTCA
    GTCTATGACA TTTATCTACC AACACATATT CAGTGTAGCA TCAAACCCCA TGCAATCATT
    ATCCTCCCCA ACACGGATGG GATGGAGCTG CTGGTGTGCT ACGAAGACGA GGGGGTTTAC
    GTTAACACGT ACGGAAGGAT CACCAAGGAC GTGGTTCTGC AGTGGGGAGA GATGCCGACA
    TCTGTAGCAT ATATCCGGTC GAATCAGACA ATGGGCTGGG GAGAGAAGGC AATAGAGATA
    CGATCTGTGG AAACTGGTCA CTTGGATGGT GTATTTATGC ACAAAAGGGC TCAGAGACTA
    AAGTTCCTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CCGTCCGCTC TGGTGGCAGC
    AGCCAGGTCT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu69246
    Clone ID Related Accession (Same CDS sequence) XM_011238530.1 , XM_011238530.2
    Accession Version XM_011238530.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3960bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011238530.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GCAGTATTGA GCCCCGGCTT
    CTTTGGGAGA GAGTGGAAAA GCTGGTCCCC AGGCCAGGCA GTGGCAGCTC TTCAGGGTCC
    AGCAACTCAG GATCCCAGCC TGGCTCCCAT CCTGGGTCTC AGAGCGGCTC CGGTGAACGC
    TTCAGAGTGA GATCATCATC CAAGTCTGAA GGTTCTCCAT CGCCACGCCA GGAAAGTGCA
    GCCAAAAAGC CTGACGATAA GAAAGAAGTA TTCCGGCCTC TCAAGCCTGC GGGAGAAGTG
    GACTTGACTG CGTTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTTCGGCC ACCTCACAAA
    GTAACAGACT ACTCATCCTC CAGTGAGGAG TCTGGGACCA CAGATGAGGA GGAGGAGGAC
    GTGGAACAGG AGGGGGCTGA TGATTCTACC TCGGGACCAG AAGACACCAG AGCAGCGTCA
    TCTCCGAACC TGAGCAACGG TGAAACAGAA TCTGTGAAAA CAATGATCGT TCATGATGAT
    GTAGAAAGTG AGCCAGCCAT GACCCCGTCC AAGGAGGGCA CCCTCATCGT CCGCCAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGGT TACTACAGAT CTCTCCATCT AGTGGGACGA CAGTGACTTC CGTGGTGGGA
    TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC ATAAGGCAAG ATCCTACTCG GAAAGGCTCA
    GTGGTCAATG TGAACCCCAC GAACACTAGG CCACAGAGTG ATACCCCCGA GATTCGTAAA
    TACAAGAAGA GATTTAACTC AGAGATCCTG TGTGCTGCCT TATGGGGAGT GAACTTGCTG
    GTTGGTACAG AGAGCGGTCT GATGCTCTTG GACAGAAGTG GCCAAGGGAA AGTATACCCT
    CTGATCAGCC GAAGACGGTT CCAGCAAATG GATGTGCTCG AAGGCCTAAA TGTCCTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TATCCTGGCT AAGAAATAAG
    ATTCTTCATA ATGATCCAGA GGTTGAGAAG AAGCAGGGGT GGACCACTGT GGGCGACTTG
    GAAGGGTGCG TGCACTATAA AGTCGTAAAA TATGAAAGAA TCAAGTTTCT GGTAATTGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCATGG GCACCGAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAACT GCTACATAAG CCATTACTGG TGGATCTCAC TGTGGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCGGGAT TCCATGCTGT TGATGTGGAT
    TCAGGCTCAG TCTATGACAT TTATCTACCA ACACATATTC AGTGTAGCAT CAAACCCCAT
    GCAATCATTA TCCTCCCCAA CACGGATGGG ATGGAGCTGC TGGTGTGCTA CGAAGACGAG
    GGGGTTTACG TTAACACGTA CGGAAGGATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG
    ATGCCGACAT CTGTAGCATA TATCCGGTCG AATCAGACAA TGGGCTGGGG AGAGAAGGCA
    ATAGAGATAC GATCTGTGGA AACTGGTCAC TTGGATGGTG TATTTATGCA CAAAAGGGCT
    CAGAGACTAA AGTTCCTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC CGTCCGCTCT
    GGTGGCAGCA GCCAGGTCTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011236832.1
    CDS329..4288
    Translation

    Target ORF information:

    RefSeq Version XM_011238530.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011238530.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GCAGTATTGA GCCCCGGCTT
    CTTTGGGAGA GAGTGGAAAA GCTGGTCCCC AGGCCAGGCA GTGGCAGCTC TTCAGGGTCC
    AGCAACTCAG GATCCCAGCC TGGCTCCCAT CCTGGGTCTC AGAGCGGCTC CGGTGAACGC
    TTCAGAGTGA GATCATCATC CAAGTCTGAA GGTTCTCCAT CGCCACGCCA GGAAAGTGCA
    GCCAAAAAGC CTGACGATAA GAAAGAAGTA TTCCGGCCTC TCAAGCCTGC GGGAGAAGTG
    GACTTGACTG CGTTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTTCGGCC ACCTCACAAA
    GTAACAGACT ACTCATCCTC CAGTGAGGAG TCTGGGACCA CAGATGAGGA GGAGGAGGAC
    GTGGAACAGG AGGGGGCTGA TGATTCTACC TCGGGACCAG AAGACACCAG AGCAGCGTCA
    TCTCCGAACC TGAGCAACGG TGAAACAGAA TCTGTGAAAA CAATGATCGT TCATGATGAT
    GTAGAAAGTG AGCCAGCCAT GACCCCGTCC AAGGAGGGCA CCCTCATCGT CCGCCAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGGT TACTACAGAT CTCTCCATCT AGTGGGACGA CAGTGACTTC CGTGGTGGGA
    TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC ATAAGGCAAG ATCCTACTCG GAAAGGCTCA
    GTGGTCAATG TGAACCCCAC GAACACTAGG CCACAGAGTG ATACCCCCGA GATTCGTAAA
    TACAAGAAGA GATTTAACTC AGAGATCCTG TGTGCTGCCT TATGGGGAGT GAACTTGCTG
    GTTGGTACAG AGAGCGGTCT GATGCTCTTG GACAGAAGTG GCCAAGGGAA AGTATACCCT
    CTGATCAGCC GAAGACGGTT CCAGCAAATG GATGTGCTCG AAGGCCTAAA TGTCCTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TATCCTGGCT AAGAAATAAG
    ATTCTTCATA ATGATCCAGA GGTTGAGAAG AAGCAGGGGT GGACCACTGT GGGCGACTTG
    GAAGGGTGCG TGCACTATAA AGTCGTAAAA TATGAAAGAA TCAAGTTTCT GGTAATTGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCATGG GCACCGAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAACT GCTACATAAG CCATTACTGG TGGATCTCAC TGTGGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCGGGAT TCCATGCTGT TGATGTGGAT
    TCAGGCTCAG TCTATGACAT TTATCTACCA ACACATATTC AGTGTAGCAT CAAACCCCAT
    GCAATCATTA TCCTCCCCAA CACGGATGGG ATGGAGCTGC TGGTGTGCTA CGAAGACGAG
    GGGGTTTACG TTAACACGTA CGGAAGGATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG
    ATGCCGACAT CTGTAGCATA TATCCGGTCG AATCAGACAA TGGGCTGGGG AGAGAAGGCA
    ATAGAGATAC GATCTGTGGA AACTGGTCAC TTGGATGGTG TATTTATGCA CAAAAGGGCT
    CAGAGACTAA AGTTCCTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC CGTCCGCTCT
    GGTGGCAGCA GCCAGGTCTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77152
    Clone ID Related Accession (Same CDS sequence) XM_017320994.2 , XM_017320994.1
    Accession Version XM_017320994.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320994.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GTATTGAGCC CCGGCTTCTT
    TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GCGGCTCCGG TGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCGC CACGCCAGGA AAGTGCAGCC
    AAAAAGCCTG ACGATAAGAA AGAAGTATTC CGGCCTCTCA AGCCTGCGGG AGAAGTGGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TTCGGCCACC TCACAAAGTA
    ACAGACTACT CATCCTCCAG TGAGGAGTCT GGGACCACAG ATGAGGAGGA GGAGGACGTG
    GAACAGGAGG GGGCTGATGA TTCTACCTCG GGACCAGAAG ACACCAGAGC AGCGTCATCT
    CCGAACCTGA GCAACGGTGA AACAGAATCT GTGAAAACAA TGATCGTTCA TGATGATGTA
    GAAAGTGAGC CAGCCATGAC CCCGTCCAAG GAGGGCACCC TCATCGTCCG CCAGAGTACA
    GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC TTCCTCCCCA
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT
    AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGGTT ACTACAGATC TCTCCATCTA GTGGGACGAC AGTGACTTCC
    GTGGTGGGAT TTTCCTGTGA TGGGCTGAGA CCAGAAGCCA TAAGGCAAGA TCCTACTCGG
    AAAGGCTCAG TGGTCAATGT GAACCCCACG AACACTAGGC CACAGAGTGA TACCCCCGAG
    ATTCGTAAAT ACAAGAAGAG ATTTAACTCA GAGATCCTGT GTGCTGCCTT ATGGGGAGTG
    AACTTGCTGG TTGGTACAGA GAGCGGTCTG ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA
    GTATACCCTC TGATCAGCCG AAGACGGTTC CAGCAAATGG ATGTGCTCGA AGGCCTAAAT
    GTCCTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT ATCCTGGCTA
    AGAAATAAGA TTCTTCATAA TGATCCAGAG GTTGAGAAGA AGCAGGGGTG GACCACTGTG
    GGCGACTTGG AAGGGTGCGT GCACTATAAA GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG
    GTAATTGCTT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CACCGAAGCC ATATCACAAA
    TTTATGGCCT TTAAGTCATT TGGAGAACTG CTACATAAGC CATTACTGGT GGATCTCACT
    GTGGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCGGGATT CCATGCTGTT
    GATGTGGATT CAGGCTCAGT CTATGACATT TATCTACCAA CACATATTCA GTGTAGCATC
    AAACCCCATG CAATCATTAT CCTCCCCAAC ACGGATGGGA TGGAGCTGCT GGTGTGCTAC
    GAAGACGAGG GGGTTTACGT TAACACGTAC GGAAGGATCA CCAAGGACGT GGTTCTGCAG
    TGGGGAGAGA TGCCGACATC TGTAGCATAT ATCCGGTCGA ATCAGACAAT GGGCTGGGGA
    GAGAAGGCAA TAGAGATACG ATCTGTGGAA ACTGGTCACT TGGATGGTGT ATTTATGCAC
    AAAAGGGCTC AGAGACTAAA GTTCCTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCC
    GTCCGCTCTG GTGGCAGCAG CCAGGTCTAT TTCATGACCT TAGGCAGGAC TTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176483.1
    CDS329..4477
    Translation

    Target ORF information:

    RefSeq Version XM_017320994.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017320994.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GTATTGAGCC CCGGCTTCTT
    TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GCGGCTCCGG TGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCGC CACGCCAGGA AAGTGCAGCC
    AAAAAGCCTG ACGATAAGAA AGAAGTATTC CGGCCTCTCA AGCCTGCGGG AGAAGTGGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TTCGGCCACC TCACAAAGTA
    ACAGACTACT CATCCTCCAG TGAGGAGTCT GGGACCACAG ATGAGGAGGA GGAGGACGTG
    GAACAGGAGG GGGCTGATGA TTCTACCTCG GGACCAGAAG ACACCAGAGC AGCGTCATCT
    CCGAACCTGA GCAACGGTGA AACAGAATCT GTGAAAACAA TGATCGTTCA TGATGATGTA
    GAAAGTGAGC CAGCCATGAC CCCGTCCAAG GAGGGCACCC TCATCGTCCG CCAGAGTACA
    GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC TTCCTCCCCA
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT
    AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGGTT ACTACAGATC TCTCCATCTA GTGGGACGAC AGTGACTTCC
    GTGGTGGGAT TTTCCTGTGA TGGGCTGAGA CCAGAAGCCA TAAGGCAAGA TCCTACTCGG
    AAAGGCTCAG TGGTCAATGT GAACCCCACG AACACTAGGC CACAGAGTGA TACCCCCGAG
    ATTCGTAAAT ACAAGAAGAG ATTTAACTCA GAGATCCTGT GTGCTGCCTT ATGGGGAGTG
    AACTTGCTGG TTGGTACAGA GAGCGGTCTG ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA
    GTATACCCTC TGATCAGCCG AAGACGGTTC CAGCAAATGG ATGTGCTCGA AGGCCTAAAT
    GTCCTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT ATCCTGGCTA
    AGAAATAAGA TTCTTCATAA TGATCCAGAG GTTGAGAAGA AGCAGGGGTG GACCACTGTG
    GGCGACTTGG AAGGGTGCGT GCACTATAAA GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG
    GTAATTGCTT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CACCGAAGCC ATATCACAAA
    TTTATGGCCT TTAAGTCATT TGGAGAACTG CTACATAAGC CATTACTGGT GGATCTCACT
    GTGGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCGGGATT CCATGCTGTT
    GATGTGGATT CAGGCTCAGT CTATGACATT TATCTACCAA CACATATTCA GTGTAGCATC
    AAACCCCATG CAATCATTAT CCTCCCCAAC ACGGATGGGA TGGAGCTGCT GGTGTGCTAC
    GAAGACGAGG GGGTTTACGT TAACACGTAC GGAAGGATCA CCAAGGACGT GGTTCTGCAG
    TGGGGAGAGA TGCCGACATC TGTAGCATAT ATCCGGTCGA ATCAGACAAT GGGCTGGGGA
    GAGAAGGCAA TAGAGATACG ATCTGTGGAA ACTGGTCACT TGGATGGTGT ATTTATGCAC
    AAAAGGGCTC AGAGACTAAA GTTCCTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCC
    GTCCGCTCTG GTGGCAGCAG CCAGGTCTAT TTCATGACCT TAGGCAGGAC TTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77152
    Clone ID Related Accession (Same CDS sequence) XM_017320994.2 , XM_017320994.1
    Accession Version XM_017320994.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GTATTGAGCC CCGGCTTCTT
    TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GCGGCTCCGG TGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCGC CACGCCAGGA AAGTGCAGCC
    AAAAAGCCTG ACGATAAGAA AGAAGTATTC CGGCCTCTCA AGCCTGCGGG AGAAGTGGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TTCGGCCACC TCACAAAGTA
    ACAGACTACT CATCCTCCAG TGAGGAGTCT GGGACCACAG ATGAGGAGGA GGAGGACGTG
    GAACAGGAGG GGGCTGATGA TTCTACCTCG GGACCAGAAG ACACCAGAGC AGCGTCATCT
    CCGAACCTGA GCAACGGTGA AACAGAATCT GTGAAAACAA TGATCGTTCA TGATGATGTA
    GAAAGTGAGC CAGCCATGAC CCCGTCCAAG GAGGGCACCC TCATCGTCCG CCAGAGTACA
    GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC TTCCTCCCCA
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT
    AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGGTT ACTACAGATC TCTCCATCTA GTGGGACGAC AGTGACTTCC
    GTGGTGGGAT TTTCCTGTGA TGGGCTGAGA CCAGAAGCCA TAAGGCAAGA TCCTACTCGG
    AAAGGCTCAG TGGTCAATGT GAACCCCACG AACACTAGGC CACAGAGTGA TACCCCCGAG
    ATTCGTAAAT ACAAGAAGAG ATTTAACTCA GAGATCCTGT GTGCTGCCTT ATGGGGAGTG
    AACTTGCTGG TTGGTACAGA GAGCGGTCTG ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA
    GTATACCCTC TGATCAGCCG AAGACGGTTC CAGCAAATGG ATGTGCTCGA AGGCCTAAAT
    GTCCTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT ATCCTGGCTA
    AGAAATAAGA TTCTTCATAA TGATCCAGAG GTTGAGAAGA AGCAGGGGTG GACCACTGTG
    GGCGACTTGG AAGGGTGCGT GCACTATAAA GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG
    GTAATTGCTT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CACCGAAGCC ATATCACAAA
    TTTATGGCCT TTAAGTCATT TGGAGAACTG CTACATAAGC CATTACTGGT GGATCTCACT
    GTGGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCGGGATT CCATGCTGTT
    GATGTGGATT CAGGCTCAGT CTATGACATT TATCTACCAA CACATATTCA GTGTAGCATC
    AAACCCCATG CAATCATTAT CCTCCCCAAC ACGGATGGGA TGGAGCTGCT GGTGTGCTAC
    GAAGACGAGG GGGTTTACGT TAACACGTAC GGAAGGATCA CCAAGGACGT GGTTCTGCAG
    TGGGGAGAGA TGCCGACATC TGTAGCATAT ATCCGGTCGA ATCAGACAAT GGGCTGGGGA
    GAGAAGGCAA TAGAGATACG ATCTGTGGAA ACTGGTCACT TGGATGGTGT ATTTATGCAC
    AAAAGGGCTC AGAGACTAAA GTTCCTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCC
    GTCCGCTCTG GTGGCAGCAG CCAGGTCTAT TTCATGACCT TAGGCAGGAC TTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176483.1
    CDS319..4467
    Translation

    Target ORF information:

    RefSeq Version XM_017320994.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017320994.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GTATTGAGCC CCGGCTTCTT
    TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCTGG CTCCCATCCT GGGTCTCAGA GCGGCTCCGG TGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGT TCTCCATCGC CACGCCAGGA AAGTGCAGCC
    AAAAAGCCTG ACGATAAGAA AGAAGTATTC CGGCCTCTCA AGCCTGCGGG AGAAGTGGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TTCGGCCACC TCACAAAGTA
    ACAGACTACT CATCCTCCAG TGAGGAGTCT GGGACCACAG ATGAGGAGGA GGAGGACGTG
    GAACAGGAGG GGGCTGATGA TTCTACCTCG GGACCAGAAG ACACCAGAGC AGCGTCATCT
    CCGAACCTGA GCAACGGTGA AACAGAATCT GTGAAAACAA TGATCGTTCA TGATGATGTA
    GAAAGTGAGC CAGCCATGAC CCCGTCCAAG GAGGGCACCC TCATCGTCCG CCAGAGTACA
    GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC TTCCTCCCCA
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT
    AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGGTT ACTACAGATC TCTCCATCTA GTGGGACGAC AGTGACTTCC
    GTGGTGGGAT TTTCCTGTGA TGGGCTGAGA CCAGAAGCCA TAAGGCAAGA TCCTACTCGG
    AAAGGCTCAG TGGTCAATGT GAACCCCACG AACACTAGGC CACAGAGTGA TACCCCCGAG
    ATTCGTAAAT ACAAGAAGAG ATTTAACTCA GAGATCCTGT GTGCTGCCTT ATGGGGAGTG
    AACTTGCTGG TTGGTACAGA GAGCGGTCTG ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA
    GTATACCCTC TGATCAGCCG AAGACGGTTC CAGCAAATGG ATGTGCTCGA AGGCCTAAAT
    GTCCTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT ATCCTGGCTA
    AGAAATAAGA TTCTTCATAA TGATCCAGAG GTTGAGAAGA AGCAGGGGTG GACCACTGTG
    GGCGACTTGG AAGGGTGCGT GCACTATAAA GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG
    GTAATTGCTT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CACCGAAGCC ATATCACAAA
    TTTATGGCCT TTAAGTCATT TGGAGAACTG CTACATAAGC CATTACTGGT GGATCTCACT
    GTGGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCGGGATT CCATGCTGTT
    GATGTGGATT CAGGCTCAGT CTATGACATT TATCTACCAA CACATATTCA GTGTAGCATC
    AAACCCCATG CAATCATTAT CCTCCCCAAC ACGGATGGGA TGGAGCTGCT GGTGTGCTAC
    GAAGACGAGG GGGTTTACGT TAACACGTAC GGAAGGATCA CCAAGGACGT GGTTCTGCAG
    TGGGGAGAGA TGCCGACATC TGTAGCATAT ATCCGGTCGA ATCAGACAAT GGGCTGGGGA
    GAGAAGGCAA TAGAGATACG ATCTGTGGAA ACTGGTCACT TGGATGGTGT ATTTATGCAC
    AAAAGGGCTC AGAGACTAAA GTTCCTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCC
    GTCCGCTCTG GTGGCAGCAG CCAGGTCTAT TTCATGACCT TAGGCAGGAC TTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77153
    Clone ID Related Accession (Same CDS sequence) XM_017320998.2 , XM_017320998.1
    Accession Version XM_017320998.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017320998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGTGGTCCC ACCTGGCATC TCTCAAGAAC AATGTCTCCC CTGTCTCGAG ATCCCATTCC
    TTCAGTGACC CTTCTCCCAA ATTCGCACAC CACCATCTCC GCTCTCAGGA CCCATGTCCA
    CCTTCCCGCA GTGAGGGGCT CAGTCAGAGC TCTGACTCTA AGTCGGAGGT GCCCGAGCCC
    ACCCAAAAGG CTTGGTCTAG ATCAGACAGT GACGAGGTGC CTCCAAGGGT TCCCGTGAGA
    ACGACCTCTC GTTCCCCTGT CCTCTCCCGT CGGGATTCCC CACTGCAGGG CGGTGGACAG
    CAGAATAGCC AAGCAGGACA GAGAAATTCC ACCAGCAGTA TTGAGCCCCG GCTTCTTTGG
    GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC
    TCAGGATCCC AGCCTGGCTC CCATCCTGGG TCTCAGAGCG GCTCCGGTGA ACGCTTCAGA
    GTGAGATCAT CATCCAAGTC TGAAGGTTCT CCATCGCCAC GCCAGGAAAG TGCAGCCAAA
    AAGCCTGACG ATAAGAAAGA AGTATTCCGG CCTCTCAAGC CTGCGGGAGA AGTGGACTTG
    ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTTC GGCCACCTCA CAAAGTAACA
    GACTACTCAT CCTCCAGTGA GGAGTCTGGG ACCACAGATG AGGAGGAGGA GGACGTGGAA
    CAGGAGGGGG CTGATGATTC TACCTCGGGA CCAGAAGACA CCAGAGCAGC GTCATCTCCG
    AACCTGAGCA ACGGTGAAAC AGAATCTGTG AAAACAATGA TCGTTCATGA TGATGTAGAA
    AGTGAGCCAG CCATGACCCC GTCCAAGGAG GGCACCCTCA TCGTCCGCCA GAGTACAGTT
    GACCAAAAGC GTGCCAGCCA TCATGAGAGC AATGGCTTTG CGGGTCGCAT TCACCTCTTG
    CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCTTC CTCCCCATCC
    TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTGCTAAT
    GAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT
    TTTATAGACC CCAGGTTACT ACAGATCTCT CCATCTAGTG GGACGACAGT GACTTCCGTG
    GTGGGATTTT CCTGTGATGG GCTGAGACCA GAAGCCATAA GGCAAGATCC TACTCGGAAA
    GGCTCAGTGG TCAATGTGAA CCCCACGAAC ACTAGGCCAC AGAGTGATAC CCCCGAGATT
    CGTAAATACA AGAAGAGATT TAACTCAGAG ATCCTGTGTG CTGCCTTATG GGGAGTGAAC
    TTGCTGGTTG GTACAGAGAG CGGTCTGATG CTCTTGGACA GAAGTGGCCA AGGGAAAGTA
    TACCCTCTGA TCAGCCGAAG ACGGTTCCAG CAAATGGATG TGCTCGAAGG CCTAAATGTC
    CTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTATC CTGGCTAAGA
    AATAAGATTC TTCATAATGA TCCAGAGGTT GAGAAGAAGC AGGGGTGGAC CACTGTGGGC
    GACTTGGAAG GGTGCGTGCA CTATAAAGTC GTAAAATATG AAAGAATCAA GTTTCTGGTA
    ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT GCATGGGCAC CGAAGCCATA TCACAAATTT
    ATGGCCTTTA AGTCATTTGG AGAACTGCTA CATAAGCCAT TACTGGTGGA TCTCACTGTG
    GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CGGGATTCCA TGCTGTTGAT
    GTGGATTCAG GCTCAGTCTA TGACATTTAT CTACCAACAC ATATTCAGTG TAGCATCAAA
    CCCCATGCAA TCATTATCCT CCCCAACACG GATGGGATGG AGCTGCTGGT GTGCTACGAA
    GACGAGGGGG TTTACGTTAA CACGTACGGA AGGATCACCA AGGACGTGGT TCTGCAGTGG
    GGAGAGATGC CGACATCTGT AGCATATATC CGGTCGAATC AGACAATGGG CTGGGGAGAG
    AAGGCAATAG AGATACGATC TGTGGAAACT GGTCACTTGG ATGGTGTATT TATGCACAAA
    AGGGCTCAGA GACTAAAGTT CCTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCCGTC
    CGCTCTGGTG GCAGCAGCCA GGTCTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176487.1
    CDS329..4474
    Translation

    Target ORF information:

    RefSeq Version XM_017320998.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017320998.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGTGGTCCC ACCTGGCATC TCTCAAGAAC AATGTCTCCC CTGTCTCGAG ATCCCATTCC
    TTCAGTGACC CTTCTCCCAA ATTCGCACAC CACCATCTCC GCTCTCAGGA CCCATGTCCA
    CCTTCCCGCA GTGAGGGGCT CAGTCAGAGC TCTGACTCTA AGTCGGAGGT GCCCGAGCCC
    ACCCAAAAGG CTTGGTCTAG ATCAGACAGT GACGAGGTGC CTCCAAGGGT TCCCGTGAGA
    ACGACCTCTC GTTCCCCTGT CCTCTCCCGT CGGGATTCCC CACTGCAGGG CGGTGGACAG
    CAGAATAGCC AAGCAGGACA GAGAAATTCC ACCAGCAGTA TTGAGCCCCG GCTTCTTTGG
    GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC
    TCAGGATCCC AGCCTGGCTC CCATCCTGGG TCTCAGAGCG GCTCCGGTGA ACGCTTCAGA
    GTGAGATCAT CATCCAAGTC TGAAGGTTCT CCATCGCCAC GCCAGGAAAG TGCAGCCAAA
    AAGCCTGACG ATAAGAAAGA AGTATTCCGG CCTCTCAAGC CTGCGGGAGA AGTGGACTTG
    ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTTC GGCCACCTCA CAAAGTAACA
    GACTACTCAT CCTCCAGTGA GGAGTCTGGG ACCACAGATG AGGAGGAGGA GGACGTGGAA
    CAGGAGGGGG CTGATGATTC TACCTCGGGA CCAGAAGACA CCAGAGCAGC GTCATCTCCG
    AACCTGAGCA ACGGTGAAAC AGAATCTGTG AAAACAATGA TCGTTCATGA TGATGTAGAA
    AGTGAGCCAG CCATGACCCC GTCCAAGGAG GGCACCCTCA TCGTCCGCCA GAGTACAGTT
    GACCAAAAGC GTGCCAGCCA TCATGAGAGC AATGGCTTTG CGGGTCGCAT TCACCTCTTG
    CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCTTC CTCCCCATCC
    TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTGCTAAT
    GAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT
    TTTATAGACC CCAGGTTACT ACAGATCTCT CCATCTAGTG GGACGACAGT GACTTCCGTG
    GTGGGATTTT CCTGTGATGG GCTGAGACCA GAAGCCATAA GGCAAGATCC TACTCGGAAA
    GGCTCAGTGG TCAATGTGAA CCCCACGAAC ACTAGGCCAC AGAGTGATAC CCCCGAGATT
    CGTAAATACA AGAAGAGATT TAACTCAGAG ATCCTGTGTG CTGCCTTATG GGGAGTGAAC
    TTGCTGGTTG GTACAGAGAG CGGTCTGATG CTCTTGGACA GAAGTGGCCA AGGGAAAGTA
    TACCCTCTGA TCAGCCGAAG ACGGTTCCAG CAAATGGATG TGCTCGAAGG CCTAAATGTC
    CTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTATC CTGGCTAAGA
    AATAAGATTC TTCATAATGA TCCAGAGGTT GAGAAGAAGC AGGGGTGGAC CACTGTGGGC
    GACTTGGAAG GGTGCGTGCA CTATAAAGTC GTAAAATATG AAAGAATCAA GTTTCTGGTA
    ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT GCATGGGCAC CGAAGCCATA TCACAAATTT
    ATGGCCTTTA AGTCATTTGG AGAACTGCTA CATAAGCCAT TACTGGTGGA TCTCACTGTG
    GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CGGGATTCCA TGCTGTTGAT
    GTGGATTCAG GCTCAGTCTA TGACATTTAT CTACCAACAC ATATTCAGTG TAGCATCAAA
    CCCCATGCAA TCATTATCCT CCCCAACACG GATGGGATGG AGCTGCTGGT GTGCTACGAA
    GACGAGGGGG TTTACGTTAA CACGTACGGA AGGATCACCA AGGACGTGGT TCTGCAGTGG
    GGAGAGATGC CGACATCTGT AGCATATATC CGGTCGAATC AGACAATGGG CTGGGGAGAG
    AAGGCAATAG AGATACGATC TGTGGAAACT GGTCACTTGG ATGGTGTATT TATGCACAAA
    AGGGCTCAGA GACTAAAGTT CCTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCCGTC
    CGCTCTGGTG GCAGCAGCCA GGTCTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77153
    Clone ID Related Accession (Same CDS sequence) XM_017320998.2 , XM_017320998.1
    Accession Version XM_017320998.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGTGGTCCC ACCTGGCATC TCTCAAGAAC AATGTCTCCC CTGTCTCGAG ATCCCATTCC
    TTCAGTGACC CTTCTCCCAA ATTCGCACAC CACCATCTCC GCTCTCAGGA CCCATGTCCA
    CCTTCCCGCA GTGAGGGGCT CAGTCAGAGC TCTGACTCTA AGTCGGAGGT GCCCGAGCCC
    ACCCAAAAGG CTTGGTCTAG ATCAGACAGT GACGAGGTGC CTCCAAGGGT TCCCGTGAGA
    ACGACCTCTC GTTCCCCTGT CCTCTCCCGT CGGGATTCCC CACTGCAGGG CGGTGGACAG
    CAGAATAGCC AAGCAGGACA GAGAAATTCC ACCAGCAGTA TTGAGCCCCG GCTTCTTTGG
    GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC
    TCAGGATCCC AGCCTGGCTC CCATCCTGGG TCTCAGAGCG GCTCCGGTGA ACGCTTCAGA
    GTGAGATCAT CATCCAAGTC TGAAGGTTCT CCATCGCCAC GCCAGGAAAG TGCAGCCAAA
    AAGCCTGACG ATAAGAAAGA AGTATTCCGG CCTCTCAAGC CTGCGGGAGA AGTGGACTTG
    ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTTC GGCCACCTCA CAAAGTAACA
    GACTACTCAT CCTCCAGTGA GGAGTCTGGG ACCACAGATG AGGAGGAGGA GGACGTGGAA
    CAGGAGGGGG CTGATGATTC TACCTCGGGA CCAGAAGACA CCAGAGCAGC GTCATCTCCG
    AACCTGAGCA ACGGTGAAAC AGAATCTGTG AAAACAATGA TCGTTCATGA TGATGTAGAA
    AGTGAGCCAG CCATGACCCC GTCCAAGGAG GGCACCCTCA TCGTCCGCCA GAGTACAGTT
    GACCAAAAGC GTGCCAGCCA TCATGAGAGC AATGGCTTTG CGGGTCGCAT TCACCTCTTG
    CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCTTC CTCCCCATCC
    TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTGCTAAT
    GAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT
    TTTATAGACC CCAGGTTACT ACAGATCTCT CCATCTAGTG GGACGACAGT GACTTCCGTG
    GTGGGATTTT CCTGTGATGG GCTGAGACCA GAAGCCATAA GGCAAGATCC TACTCGGAAA
    GGCTCAGTGG TCAATGTGAA CCCCACGAAC ACTAGGCCAC AGAGTGATAC CCCCGAGATT
    CGTAAATACA AGAAGAGATT TAACTCAGAG ATCCTGTGTG CTGCCTTATG GGGAGTGAAC
    TTGCTGGTTG GTACAGAGAG CGGTCTGATG CTCTTGGACA GAAGTGGCCA AGGGAAAGTA
    TACCCTCTGA TCAGCCGAAG ACGGTTCCAG CAAATGGATG TGCTCGAAGG CCTAAATGTC
    CTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTATC CTGGCTAAGA
    AATAAGATTC TTCATAATGA TCCAGAGGTT GAGAAGAAGC AGGGGTGGAC CACTGTGGGC
    GACTTGGAAG GGTGCGTGCA CTATAAAGTC GTAAAATATG AAAGAATCAA GTTTCTGGTA
    ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT GCATGGGCAC CGAAGCCATA TCACAAATTT
    ATGGCCTTTA AGTCATTTGG AGAACTGCTA CATAAGCCAT TACTGGTGGA TCTCACTGTG
    GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CGGGATTCCA TGCTGTTGAT
    GTGGATTCAG GCTCAGTCTA TGACATTTAT CTACCAACAC ATATTCAGTG TAGCATCAAA
    CCCCATGCAA TCATTATCCT CCCCAACACG GATGGGATGG AGCTGCTGGT GTGCTACGAA
    GACGAGGGGG TTTACGTTAA CACGTACGGA AGGATCACCA AGGACGTGGT TCTGCAGTGG
    GGAGAGATGC CGACATCTGT AGCATATATC CGGTCGAATC AGACAATGGG CTGGGGAGAG
    AAGGCAATAG AGATACGATC TGTGGAAACT GGTCACTTGG ATGGTGTATT TATGCACAAA
    AGGGCTCAGA GACTAAAGTT CCTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCCGTC
    CGCTCTGGTG GCAGCAGCCA GGTCTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176487.1
    CDS319..4464
    Translation

    Target ORF information:

    RefSeq Version XM_017320998.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017320998.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGTGGTCCC ACCTGGCATC TCTCAAGAAC AATGTCTCCC CTGTCTCGAG ATCCCATTCC
    TTCAGTGACC CTTCTCCCAA ATTCGCACAC CACCATCTCC GCTCTCAGGA CCCATGTCCA
    CCTTCCCGCA GTGAGGGGCT CAGTCAGAGC TCTGACTCTA AGTCGGAGGT GCCCGAGCCC
    ACCCAAAAGG CTTGGTCTAG ATCAGACAGT GACGAGGTGC CTCCAAGGGT TCCCGTGAGA
    ACGACCTCTC GTTCCCCTGT CCTCTCCCGT CGGGATTCCC CACTGCAGGG CGGTGGACAG
    CAGAATAGCC AAGCAGGACA GAGAAATTCC ACCAGCAGTA TTGAGCCCCG GCTTCTTTGG
    GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC
    TCAGGATCCC AGCCTGGCTC CCATCCTGGG TCTCAGAGCG GCTCCGGTGA ACGCTTCAGA
    GTGAGATCAT CATCCAAGTC TGAAGGTTCT CCATCGCCAC GCCAGGAAAG TGCAGCCAAA
    AAGCCTGACG ATAAGAAAGA AGTATTCCGG CCTCTCAAGC CTGCGGGAGA AGTGGACTTG
    ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTTC GGCCACCTCA CAAAGTAACA
    GACTACTCAT CCTCCAGTGA GGAGTCTGGG ACCACAGATG AGGAGGAGGA GGACGTGGAA
    CAGGAGGGGG CTGATGATTC TACCTCGGGA CCAGAAGACA CCAGAGCAGC GTCATCTCCG
    AACCTGAGCA ACGGTGAAAC AGAATCTGTG AAAACAATGA TCGTTCATGA TGATGTAGAA
    AGTGAGCCAG CCATGACCCC GTCCAAGGAG GGCACCCTCA TCGTCCGCCA GAGTACAGTT
    GACCAAAAGC GTGCCAGCCA TCATGAGAGC AATGGCTTTG CGGGTCGCAT TCACCTCTTG
    CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCTTC CTCCCCATCC
    TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTGCTAAT
    GAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT
    TTTATAGACC CCAGGTTACT ACAGATCTCT CCATCTAGTG GGACGACAGT GACTTCCGTG
    GTGGGATTTT CCTGTGATGG GCTGAGACCA GAAGCCATAA GGCAAGATCC TACTCGGAAA
    GGCTCAGTGG TCAATGTGAA CCCCACGAAC ACTAGGCCAC AGAGTGATAC CCCCGAGATT
    CGTAAATACA AGAAGAGATT TAACTCAGAG ATCCTGTGTG CTGCCTTATG GGGAGTGAAC
    TTGCTGGTTG GTACAGAGAG CGGTCTGATG CTCTTGGACA GAAGTGGCCA AGGGAAAGTA
    TACCCTCTGA TCAGCCGAAG ACGGTTCCAG CAAATGGATG TGCTCGAAGG CCTAAATGTC
    CTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTATC CTGGCTAAGA
    AATAAGATTC TTCATAATGA TCCAGAGGTT GAGAAGAAGC AGGGGTGGAC CACTGTGGGC
    GACTTGGAAG GGTGCGTGCA CTATAAAGTC GTAAAATATG AAAGAATCAA GTTTCTGGTA
    ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT GCATGGGCAC CGAAGCCATA TCACAAATTT
    ATGGCCTTTA AGTCATTTGG AGAACTGCTA CATAAGCCAT TACTGGTGGA TCTCACTGTG
    GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CGGGATTCCA TGCTGTTGAT
    GTGGATTCAG GCTCAGTCTA TGACATTTAT CTACCAACAC ATATTCAGTG TAGCATCAAA
    CCCCATGCAA TCATTATCCT CCCCAACACG GATGGGATGG AGCTGCTGGT GTGCTACGAA
    GACGAGGGGG TTTACGTTAA CACGTACGGA AGGATCACCA AGGACGTGGT TCTGCAGTGG
    GGAGAGATGC CGACATCTGT AGCATATATC CGGTCGAATC AGACAATGGG CTGGGGAGAG
    AAGGCAATAG AGATACGATC TGTGGAAACT GGTCACTTGG ATGGTGTATT TATGCACAAA
    AGGGCTCAGA GACTAAAGTT CCTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCCGTC
    CGCTCTGGTG GCAGCAGCCA GGTCTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77154
    Clone ID Related Accession (Same CDS sequence) XM_017321001.1 , XM_017321001.2
    Accession Version XM_017321001.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GCAGTATTGA GCCCCGGCTT
    CTTTGGGAGA GAGTGGAAAA GCTGGTCCCC AGGCCAGGCA GTGGCAGCTC TTCAGGGTCC
    AGCAACTCAG GATCCCAGCC TGGCTCCCAT CCTGGGTCTC AGAGCGGCTC CGGTGAACGC
    TTCAGAGTGA GATCATCATC CAAGTCTGAA GGTTCTCCAT CGCCACGCCA GGAAAGTGCA
    GCCAAAAAGC CTGACGATAA GAAAGAAGTA TTCCGGCCTC TCAAGCCTGC GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTTCGGC CACCTCACAA AGTAACAGAC
    TACTCATCCT CCAGTGAGGA GTCTGGGACC ACAGATGAGG AGGAGGAGGA CGTGGAACAG
    GAGGGGGCTG ATGATTCTAC CTCGGGACCA GAAGACACCA GAGCAGCGTC ATCTCCGAAC
    CTGAGCAACG GTGAAACAGA ATCTGTGAAA ACAATGATCG TTCATGATGA TGTAGAAAGT
    GAGCCAGCCA TGACCCCGTC CAAGGAGGGC ACCCTCATCG TCCGCCAGAG TACAGTTGAC
    CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA
    GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCTTCCTC CCCATCCTCC
    AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG
    ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT
    ATAGACCCCA GGTTACTACA GATCTCTCCA TCTAGTGGGA CGACAGTGAC TTCCGTGGTG
    GGATTTTCCT GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC
    TCAGTGGTCA ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT
    AAATACAAGA AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAACTTG
    CTGGTTGGTA CAGAGAGCGG TCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTATAC
    CCTCTGATCA GCCGAAGACG GTTCCAGCAA ATGGATGTGC TCGAAGGCCT AAATGTCCTG
    GTGACAATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTATCCTG GCTAAGAAAT
    AAGATTCTTC ATAATGATCC AGAGGTTGAG AAGAAGCAGG GGTGGACCAC TGTGGGCGAC
    TTGGAAGGGT GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATT
    GCTTTGAAGA GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG
    GCCTTTAAGT CATTTGGAGA ACTGCTACAT AAGCCATTAC TGGTGGATCT CACTGTGGAG
    GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCGG GATTCCATGC TGTTGATGTG
    GATTCAGGCT CAGTCTATGA CATTTATCTA CCAACACATA TTCAGTGTAG CATCAAACCC
    CATGCAATCA TTATCCTCCC CAACACGGAT GGGATGGAGC TGCTGGTGTG CTACGAAGAC
    GAGGGGGTTT ACGTTAACAC GTACGGAAGG ATCACCAAGG ACGTGGTTCT GCAGTGGGGA
    GAGATGCCGA CATCTGTAGC ATATATCCGG TCGAATCAGA CAATGGGCTG GGGAGAGAAG
    GCAATAGAGA TACGATCTGT GGAAACTGGT CACTTGGATG GTGTATTTAT GCACAAAAGG
    GCTCAGAGAC TAAAGTTCCT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCCGTCCGC
    TCTGGTGGCA GCAGCCAGGT CTATTTCATG ACCTTAGGCA GGACTTCTCT TCTGAGCTGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176490.1
    CDS319..4461
    Translation

    Target ORF information:

    RefSeq Version XM_017321001.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321001.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GCAGTATTGA GCCCCGGCTT
    CTTTGGGAGA GAGTGGAAAA GCTGGTCCCC AGGCCAGGCA GTGGCAGCTC TTCAGGGTCC
    AGCAACTCAG GATCCCAGCC TGGCTCCCAT CCTGGGTCTC AGAGCGGCTC CGGTGAACGC
    TTCAGAGTGA GATCATCATC CAAGTCTGAA GGTTCTCCAT CGCCACGCCA GGAAAGTGCA
    GCCAAAAAGC CTGACGATAA GAAAGAAGTA TTCCGGCCTC TCAAGCCTGC GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTTCGGC CACCTCACAA AGTAACAGAC
    TACTCATCCT CCAGTGAGGA GTCTGGGACC ACAGATGAGG AGGAGGAGGA CGTGGAACAG
    GAGGGGGCTG ATGATTCTAC CTCGGGACCA GAAGACACCA GAGCAGCGTC ATCTCCGAAC
    CTGAGCAACG GTGAAACAGA ATCTGTGAAA ACAATGATCG TTCATGATGA TGTAGAAAGT
    GAGCCAGCCA TGACCCCGTC CAAGGAGGGC ACCCTCATCG TCCGCCAGAG TACAGTTGAC
    CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA
    GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCTTCCTC CCCATCCTCC
    AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG
    ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT
    ATAGACCCCA GGTTACTACA GATCTCTCCA TCTAGTGGGA CGACAGTGAC TTCCGTGGTG
    GGATTTTCCT GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC
    TCAGTGGTCA ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT
    AAATACAAGA AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAACTTG
    CTGGTTGGTA CAGAGAGCGG TCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTATAC
    CCTCTGATCA GCCGAAGACG GTTCCAGCAA ATGGATGTGC TCGAAGGCCT AAATGTCCTG
    GTGACAATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTATCCTG GCTAAGAAAT
    AAGATTCTTC ATAATGATCC AGAGGTTGAG AAGAAGCAGG GGTGGACCAC TGTGGGCGAC
    TTGGAAGGGT GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATT
    GCTTTGAAGA GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG
    GCCTTTAAGT CATTTGGAGA ACTGCTACAT AAGCCATTAC TGGTGGATCT CACTGTGGAG
    GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCGG GATTCCATGC TGTTGATGTG
    GATTCAGGCT CAGTCTATGA CATTTATCTA CCAACACATA TTCAGTGTAG CATCAAACCC
    CATGCAATCA TTATCCTCCC CAACACGGAT GGGATGGAGC TGCTGGTGTG CTACGAAGAC
    GAGGGGGTTT ACGTTAACAC GTACGGAAGG ATCACCAAGG ACGTGGTTCT GCAGTGGGGA
    GAGATGCCGA CATCTGTAGC ATATATCCGG TCGAATCAGA CAATGGGCTG GGGAGAGAAG
    GCAATAGAGA TACGATCTGT GGAAACTGGT CACTTGGATG GTGTATTTAT GCACAAAAGG
    GCTCAGAGAC TAAAGTTCCT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCCGTCCGC
    TCTGGTGGCA GCAGCCAGGT CTATTTCATG ACCTTAGGCA GGACTTCTCT TCTGAGCTGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77154
    Clone ID Related Accession (Same CDS sequence) XM_017321001.1 , XM_017321001.2
    Accession Version XM_017321001.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GCAGTATTGA GCCCCGGCTT
    CTTTGGGAGA GAGTGGAAAA GCTGGTCCCC AGGCCAGGCA GTGGCAGCTC TTCAGGGTCC
    AGCAACTCAG GATCCCAGCC TGGCTCCCAT CCTGGGTCTC AGAGCGGCTC CGGTGAACGC
    TTCAGAGTGA GATCATCATC CAAGTCTGAA GGTTCTCCAT CGCCACGCCA GGAAAGTGCA
    GCCAAAAAGC CTGACGATAA GAAAGAAGTA TTCCGGCCTC TCAAGCCTGC GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTTCGGC CACCTCACAA AGTAACAGAC
    TACTCATCCT CCAGTGAGGA GTCTGGGACC ACAGATGAGG AGGAGGAGGA CGTGGAACAG
    GAGGGGGCTG ATGATTCTAC CTCGGGACCA GAAGACACCA GAGCAGCGTC ATCTCCGAAC
    CTGAGCAACG GTGAAACAGA ATCTGTGAAA ACAATGATCG TTCATGATGA TGTAGAAAGT
    GAGCCAGCCA TGACCCCGTC CAAGGAGGGC ACCCTCATCG TCCGCCAGAG TACAGTTGAC
    CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA
    GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCTTCCTC CCCATCCTCC
    AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG
    ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT
    ATAGACCCCA GGTTACTACA GATCTCTCCA TCTAGTGGGA CGACAGTGAC TTCCGTGGTG
    GGATTTTCCT GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC
    TCAGTGGTCA ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT
    AAATACAAGA AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAACTTG
    CTGGTTGGTA CAGAGAGCGG TCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTATAC
    CCTCTGATCA GCCGAAGACG GTTCCAGCAA ATGGATGTGC TCGAAGGCCT AAATGTCCTG
    GTGACAATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTATCCTG GCTAAGAAAT
    AAGATTCTTC ATAATGATCC AGAGGTTGAG AAGAAGCAGG GGTGGACCAC TGTGGGCGAC
    TTGGAAGGGT GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATT
    GCTTTGAAGA GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG
    GCCTTTAAGT CATTTGGAGA ACTGCTACAT AAGCCATTAC TGGTGGATCT CACTGTGGAG
    GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCGG GATTCCATGC TGTTGATGTG
    GATTCAGGCT CAGTCTATGA CATTTATCTA CCAACACATA TTCAGTGTAG CATCAAACCC
    CATGCAATCA TTATCCTCCC CAACACGGAT GGGATGGAGC TGCTGGTGTG CTACGAAGAC
    GAGGGGGTTT ACGTTAACAC GTACGGAAGG ATCACCAAGG ACGTGGTTCT GCAGTGGGGA
    GAGATGCCGA CATCTGTAGC ATATATCCGG TCGAATCAGA CAATGGGCTG GGGAGAGAAG
    GCAATAGAGA TACGATCTGT GGAAACTGGT CACTTGGATG GTGTATTTAT GCACAAAAGG
    GCTCAGAGAC TAAAGTTCCT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCCGTCCGC
    TCTGGTGGCA GCAGCCAGGT CTATTTCATG ACCTTAGGCA GGACTTCTCT TCTGAGCTGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176490.1
    CDS329..4471
    Translation

    Target ORF information:

    RefSeq Version XM_017321001.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321001.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GCAGTATTGA GCCCCGGCTT
    CTTTGGGAGA GAGTGGAAAA GCTGGTCCCC AGGCCAGGCA GTGGCAGCTC TTCAGGGTCC
    AGCAACTCAG GATCCCAGCC TGGCTCCCAT CCTGGGTCTC AGAGCGGCTC CGGTGAACGC
    TTCAGAGTGA GATCATCATC CAAGTCTGAA GGTTCTCCAT CGCCACGCCA GGAAAGTGCA
    GCCAAAAAGC CTGACGATAA GAAAGAAGTA TTCCGGCCTC TCAAGCCTGC GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTTCGGC CACCTCACAA AGTAACAGAC
    TACTCATCCT CCAGTGAGGA GTCTGGGACC ACAGATGAGG AGGAGGAGGA CGTGGAACAG
    GAGGGGGCTG ATGATTCTAC CTCGGGACCA GAAGACACCA GAGCAGCGTC ATCTCCGAAC
    CTGAGCAACG GTGAAACAGA ATCTGTGAAA ACAATGATCG TTCATGATGA TGTAGAAAGT
    GAGCCAGCCA TGACCCCGTC CAAGGAGGGC ACCCTCATCG TCCGCCAGAG TACAGTTGAC
    CAAAAGCGTG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA
    GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCTTCCTC CCCATCCTCC
    AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TGCTAATGAG
    ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT
    ATAGACCCCA GGTTACTACA GATCTCTCCA TCTAGTGGGA CGACAGTGAC TTCCGTGGTG
    GGATTTTCCT GTGATGGGCT GAGACCAGAA GCCATAAGGC AAGATCCTAC TCGGAAAGGC
    TCAGTGGTCA ATGTGAACCC CACGAACACT AGGCCACAGA GTGATACCCC CGAGATTCGT
    AAATACAAGA AGAGATTTAA CTCAGAGATC CTGTGTGCTG CCTTATGGGG AGTGAACTTG
    CTGGTTGGTA CAGAGAGCGG TCTGATGCTC TTGGACAGAA GTGGCCAAGG GAAAGTATAC
    CCTCTGATCA GCCGAAGACG GTTCCAGCAA ATGGATGTGC TCGAAGGCCT AAATGTCCTG
    GTGACAATAT CTGGCAAAAA GGATAAGTTA CGTGTCTACT ATTTATCCTG GCTAAGAAAT
    AAGATTCTTC ATAATGATCC AGAGGTTGAG AAGAAGCAGG GGTGGACCAC TGTGGGCGAC
    TTGGAAGGGT GCGTGCACTA TAAAGTCGTA AAATATGAAA GAATCAAGTT TCTGGTAATT
    GCTTTGAAGA GTTCTGTGGA AGTCTATGCA TGGGCACCGA AGCCATATCA CAAATTTATG
    GCCTTTAAGT CATTTGGAGA ACTGCTACAT AAGCCATTAC TGGTGGATCT CACTGTGGAG
    GAAGGCCAGA GGTTGAAAGT GATCTATGGA TCCTGTGCGG GATTCCATGC TGTTGATGTG
    GATTCAGGCT CAGTCTATGA CATTTATCTA CCAACACATA TTCAGTGTAG CATCAAACCC
    CATGCAATCA TTATCCTCCC CAACACGGAT GGGATGGAGC TGCTGGTGTG CTACGAAGAC
    GAGGGGGTTT ACGTTAACAC GTACGGAAGG ATCACCAAGG ACGTGGTTCT GCAGTGGGGA
    GAGATGCCGA CATCTGTAGC ATATATCCGG TCGAATCAGA CAATGGGCTG GGGAGAGAAG
    GCAATAGAGA TACGATCTGT GGAAACTGGT CACTTGGATG GTGTATTTAT GCACAAAAGG
    GCTCAGAGAC TAAAGTTCCT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCCGTCCGC
    TCTGGTGGCA GCAGCCAGGT CTATTTCATG ACCTTAGGCA GGACTTCTCT TCTGAGCTGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77155
    Clone ID Related Accession (Same CDS sequence) XM_017321002.2 , XM_017321002.1
    Accession Version XM_017321002.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321002.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTACAGTGGT CCCACCTGGC ATCTCTCAAG
    AACAATGTCT CCCCTGTCTC GAGATCCCAT TCCTTCAGTG ACCCTTCTCC CAAATTCGCA
    CACCACCATC TCCGCTCTCA GGACCCATGT CCACCTTCCC GCAGTGAGGG GCTCAGTCAG
    AGCTCTGACT CTAAGTCGGA GGTGCCCGAG CCCACCCAAA AGGCTTGGTC TAGATCAGAC
    AGTGACGAGG TGCCTCCAAG GGTTCCCGTG AGAACGACCT CTCGTTCCCC TGTCCTCTCC
    CGTCGGGATT CCCCACTGCA GGGCGGTGGA CAGCAGAATA GCCAAGCAGG ACAGAGAAAT
    TCCACCAGCA GTATTGAGCC CCGGCTTCTT TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG
    CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC AACTCAGGAT CCCAGCCTGG CTCCCATCCT
    GGGTCTCAGA GCGGCTCCGG TGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGT
    TCTCCATCGC CACGCCAGGA AAGTGCAGCC AAAAAGCCTG ACGATAAGAA AGAAGTATTC
    CGGCCTCTCA AGCCTGCGGG AGAAGTGGAC TTGACTGCGT TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TTCGGCCACC TCACAAAGTA ACAGACTACT CATCCTCCAG TGAGGAGTCT
    GGGACCACAG ATGAGGAGGA GGAGGACGTG GAACAGGAGG GGGCTGATGA TTCTACCTCG
    GGACCAGAAG ACACCAGAGC AGCGTCATCT CCGAACCTGA GCAACGGTGA AACAGAATCT
    GTGAAAACAA TGATCGTTCA TGATGATGTA GAAAGTGAGC CAGCCATGAC CCCGTCCAAG
    GAGGGCACCC TCATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCGGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC TTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGGTT ACTACAGATC
    TCTCCATCTA GTGGGACGAC AGTGACTTCC GTGGTGGGAT TTTCCTGTGA TGGGCTGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACTCGG AAAGGCTCAG TGGTCAATGT GAACCCCACG
    AACACTAGGC CACAGAGTGA TACCCCCGAG ATTCGTAAAT ACAAGAAGAG ATTTAACTCA
    GAGATCCTGT GTGCTGCCTT ATGGGGAGTG AACTTGCTGG TTGGTACAGA GAGCGGTCTG
    ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA GTATACCCTC TGATCAGCCG AAGACGGTTC
    CAGCAAATGG ATGTGCTCGA AGGCCTAAAT GTCCTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT ATCCTGGCTA AGAAATAAGA TTCTTCATAA TGATCCAGAG
    GTTGAGAAGA AGCAGGGGTG GACCACTGTG GGCGACTTGG AAGGGTGCGT GCACTATAAA
    GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG GTAATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCATGGG CACCGAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAACTG
    CTACATAAGC CATTACTGGT GGATCTCACT GTGGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCGGGATT CCATGCTGTT GATGTGGATT CAGGCTCAGT CTATGACATT
    TATCTACCAA CACATATTCA GTGTAGCATC AAACCCCATG CAATCATTAT CCTCCCCAAC
    ACGGATGGGA TGGAGCTGCT GGTGTGCTAC GAAGACGAGG GGGTTTACGT TAACACGTAC
    GGAAGGATCA CCAAGGACGT GGTTCTGCAG TGGGGAGAGA TGCCGACATC TGTAGCATAT
    ATCCGGTCGA ATCAGACAAT GGGCTGGGGA GAGAAGGCAA TAGAGATACG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT ATTTATGCAC AAAAGGGCTC AGAGACTAAA GTTCCTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCC GTCCGCTCTG GTGGCAGCAG CCAGGTCTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176491.1
    CDS329..4387
    Translation

    Target ORF information:

    RefSeq Version XM_017321002.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321002.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTACAGTGGT CCCACCTGGC ATCTCTCAAG
    AACAATGTCT CCCCTGTCTC GAGATCCCAT TCCTTCAGTG ACCCTTCTCC CAAATTCGCA
    CACCACCATC TCCGCTCTCA GGACCCATGT CCACCTTCCC GCAGTGAGGG GCTCAGTCAG
    AGCTCTGACT CTAAGTCGGA GGTGCCCGAG CCCACCCAAA AGGCTTGGTC TAGATCAGAC
    AGTGACGAGG TGCCTCCAAG GGTTCCCGTG AGAACGACCT CTCGTTCCCC TGTCCTCTCC
    CGTCGGGATT CCCCACTGCA GGGCGGTGGA CAGCAGAATA GCCAAGCAGG ACAGAGAAAT
    TCCACCAGCA GTATTGAGCC CCGGCTTCTT TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG
    CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC AACTCAGGAT CCCAGCCTGG CTCCCATCCT
    GGGTCTCAGA GCGGCTCCGG TGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGT
    TCTCCATCGC CACGCCAGGA AAGTGCAGCC AAAAAGCCTG ACGATAAGAA AGAAGTATTC
    CGGCCTCTCA AGCCTGCGGG AGAAGTGGAC TTGACTGCGT TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TTCGGCCACC TCACAAAGTA ACAGACTACT CATCCTCCAG TGAGGAGTCT
    GGGACCACAG ATGAGGAGGA GGAGGACGTG GAACAGGAGG GGGCTGATGA TTCTACCTCG
    GGACCAGAAG ACACCAGAGC AGCGTCATCT CCGAACCTGA GCAACGGTGA AACAGAATCT
    GTGAAAACAA TGATCGTTCA TGATGATGTA GAAAGTGAGC CAGCCATGAC CCCGTCCAAG
    GAGGGCACCC TCATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCGGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC TTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGGTT ACTACAGATC
    TCTCCATCTA GTGGGACGAC AGTGACTTCC GTGGTGGGAT TTTCCTGTGA TGGGCTGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACTCGG AAAGGCTCAG TGGTCAATGT GAACCCCACG
    AACACTAGGC CACAGAGTGA TACCCCCGAG ATTCGTAAAT ACAAGAAGAG ATTTAACTCA
    GAGATCCTGT GTGCTGCCTT ATGGGGAGTG AACTTGCTGG TTGGTACAGA GAGCGGTCTG
    ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA GTATACCCTC TGATCAGCCG AAGACGGTTC
    CAGCAAATGG ATGTGCTCGA AGGCCTAAAT GTCCTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT ATCCTGGCTA AGAAATAAGA TTCTTCATAA TGATCCAGAG
    GTTGAGAAGA AGCAGGGGTG GACCACTGTG GGCGACTTGG AAGGGTGCGT GCACTATAAA
    GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG GTAATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCATGGG CACCGAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAACTG
    CTACATAAGC CATTACTGGT GGATCTCACT GTGGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCGGGATT CCATGCTGTT GATGTGGATT CAGGCTCAGT CTATGACATT
    TATCTACCAA CACATATTCA GTGTAGCATC AAACCCCATG CAATCATTAT CCTCCCCAAC
    ACGGATGGGA TGGAGCTGCT GGTGTGCTAC GAAGACGAGG GGGTTTACGT TAACACGTAC
    GGAAGGATCA CCAAGGACGT GGTTCTGCAG TGGGGAGAGA TGCCGACATC TGTAGCATAT
    ATCCGGTCGA ATCAGACAAT GGGCTGGGGA GAGAAGGCAA TAGAGATACG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT ATTTATGCAC AAAAGGGCTC AGAGACTAAA GTTCCTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCC GTCCGCTCTG GTGGCAGCAG CCAGGTCTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77155
    Clone ID Related Accession (Same CDS sequence) XM_017321002.2 , XM_017321002.1
    Accession Version XM_017321002.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTACAGTGGT CCCACCTGGC ATCTCTCAAG
    AACAATGTCT CCCCTGTCTC GAGATCCCAT TCCTTCAGTG ACCCTTCTCC CAAATTCGCA
    CACCACCATC TCCGCTCTCA GGACCCATGT CCACCTTCCC GCAGTGAGGG GCTCAGTCAG
    AGCTCTGACT CTAAGTCGGA GGTGCCCGAG CCCACCCAAA AGGCTTGGTC TAGATCAGAC
    AGTGACGAGG TGCCTCCAAG GGTTCCCGTG AGAACGACCT CTCGTTCCCC TGTCCTCTCC
    CGTCGGGATT CCCCACTGCA GGGCGGTGGA CAGCAGAATA GCCAAGCAGG ACAGAGAAAT
    TCCACCAGCA GTATTGAGCC CCGGCTTCTT TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG
    CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC AACTCAGGAT CCCAGCCTGG CTCCCATCCT
    GGGTCTCAGA GCGGCTCCGG TGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGT
    TCTCCATCGC CACGCCAGGA AAGTGCAGCC AAAAAGCCTG ACGATAAGAA AGAAGTATTC
    CGGCCTCTCA AGCCTGCGGG AGAAGTGGAC TTGACTGCGT TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TTCGGCCACC TCACAAAGTA ACAGACTACT CATCCTCCAG TGAGGAGTCT
    GGGACCACAG ATGAGGAGGA GGAGGACGTG GAACAGGAGG GGGCTGATGA TTCTACCTCG
    GGACCAGAAG ACACCAGAGC AGCGTCATCT CCGAACCTGA GCAACGGTGA AACAGAATCT
    GTGAAAACAA TGATCGTTCA TGATGATGTA GAAAGTGAGC CAGCCATGAC CCCGTCCAAG
    GAGGGCACCC TCATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCGGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC TTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGGTT ACTACAGATC
    TCTCCATCTA GTGGGACGAC AGTGACTTCC GTGGTGGGAT TTTCCTGTGA TGGGCTGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACTCGG AAAGGCTCAG TGGTCAATGT GAACCCCACG
    AACACTAGGC CACAGAGTGA TACCCCCGAG ATTCGTAAAT ACAAGAAGAG ATTTAACTCA
    GAGATCCTGT GTGCTGCCTT ATGGGGAGTG AACTTGCTGG TTGGTACAGA GAGCGGTCTG
    ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA GTATACCCTC TGATCAGCCG AAGACGGTTC
    CAGCAAATGG ATGTGCTCGA AGGCCTAAAT GTCCTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT ATCCTGGCTA AGAAATAAGA TTCTTCATAA TGATCCAGAG
    GTTGAGAAGA AGCAGGGGTG GACCACTGTG GGCGACTTGG AAGGGTGCGT GCACTATAAA
    GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG GTAATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCATGGG CACCGAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAACTG
    CTACATAAGC CATTACTGGT GGATCTCACT GTGGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCGGGATT CCATGCTGTT GATGTGGATT CAGGCTCAGT CTATGACATT
    TATCTACCAA CACATATTCA GTGTAGCATC AAACCCCATG CAATCATTAT CCTCCCCAAC
    ACGGATGGGA TGGAGCTGCT GGTGTGCTAC GAAGACGAGG GGGTTTACGT TAACACGTAC
    GGAAGGATCA CCAAGGACGT GGTTCTGCAG TGGGGAGAGA TGCCGACATC TGTAGCATAT
    ATCCGGTCGA ATCAGACAAT GGGCTGGGGA GAGAAGGCAA TAGAGATACG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT ATTTATGCAC AAAAGGGCTC AGAGACTAAA GTTCCTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCC GTCCGCTCTG GTGGCAGCAG CCAGGTCTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176491.1
    CDS319..4377
    Translation

    Target ORF information:

    RefSeq Version XM_017321002.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321002.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTACAGTGGT CCCACCTGGC ATCTCTCAAG
    AACAATGTCT CCCCTGTCTC GAGATCCCAT TCCTTCAGTG ACCCTTCTCC CAAATTCGCA
    CACCACCATC TCCGCTCTCA GGACCCATGT CCACCTTCCC GCAGTGAGGG GCTCAGTCAG
    AGCTCTGACT CTAAGTCGGA GGTGCCCGAG CCCACCCAAA AGGCTTGGTC TAGATCAGAC
    AGTGACGAGG TGCCTCCAAG GGTTCCCGTG AGAACGACCT CTCGTTCCCC TGTCCTCTCC
    CGTCGGGATT CCCCACTGCA GGGCGGTGGA CAGCAGAATA GCCAAGCAGG ACAGAGAAAT
    TCCACCAGCA GTATTGAGCC CCGGCTTCTT TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG
    CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC AACTCAGGAT CCCAGCCTGG CTCCCATCCT
    GGGTCTCAGA GCGGCTCCGG TGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGT
    TCTCCATCGC CACGCCAGGA AAGTGCAGCC AAAAAGCCTG ACGATAAGAA AGAAGTATTC
    CGGCCTCTCA AGCCTGCGGG AGAAGTGGAC TTGACTGCGT TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TTCGGCCACC TCACAAAGTA ACAGACTACT CATCCTCCAG TGAGGAGTCT
    GGGACCACAG ATGAGGAGGA GGAGGACGTG GAACAGGAGG GGGCTGATGA TTCTACCTCG
    GGACCAGAAG ACACCAGAGC AGCGTCATCT CCGAACCTGA GCAACGGTGA AACAGAATCT
    GTGAAAACAA TGATCGTTCA TGATGATGTA GAAAGTGAGC CAGCCATGAC CCCGTCCAAG
    GAGGGCACCC TCATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCGGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC TTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGGTT ACTACAGATC
    TCTCCATCTA GTGGGACGAC AGTGACTTCC GTGGTGGGAT TTTCCTGTGA TGGGCTGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACTCGG AAAGGCTCAG TGGTCAATGT GAACCCCACG
    AACACTAGGC CACAGAGTGA TACCCCCGAG ATTCGTAAAT ACAAGAAGAG ATTTAACTCA
    GAGATCCTGT GTGCTGCCTT ATGGGGAGTG AACTTGCTGG TTGGTACAGA GAGCGGTCTG
    ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA GTATACCCTC TGATCAGCCG AAGACGGTTC
    CAGCAAATGG ATGTGCTCGA AGGCCTAAAT GTCCTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT ATCCTGGCTA AGAAATAAGA TTCTTCATAA TGATCCAGAG
    GTTGAGAAGA AGCAGGGGTG GACCACTGTG GGCGACTTGG AAGGGTGCGT GCACTATAAA
    GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG GTAATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCATGGG CACCGAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAACTG
    CTACATAAGC CATTACTGGT GGATCTCACT GTGGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCGGGATT CCATGCTGTT GATGTGGATT CAGGCTCAGT CTATGACATT
    TATCTACCAA CACATATTCA GTGTAGCATC AAACCCCATG CAATCATTAT CCTCCCCAAC
    ACGGATGGGA TGGAGCTGCT GGTGTGCTAC GAAGACGAGG GGGTTTACGT TAACACGTAC
    GGAAGGATCA CCAAGGACGT GGTTCTGCAG TGGGGAGAGA TGCCGACATC TGTAGCATAT
    ATCCGGTCGA ATCAGACAAT GGGCTGGGGA GAGAAGGCAA TAGAGATACG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT ATTTATGCAC AAAAGGGCTC AGAGACTAAA GTTCCTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCC GTCCGCTCTG GTGGCAGCAG CCAGGTCTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77156
    Clone ID Related Accession (Same CDS sequence) XM_017321004.1 , XM_017321004.2
    Accession Version XM_017321004.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GTGGTCCCAC CTGGCATCTC TCAAGAACAA TGTCTCCCCT
    GTCTCGAGAT CCCATTCCTT CAGTGACCCT TCTCCCAAAT TCGCACACCA CCATCTCCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGGGCTCA GTCAGAGCTC TGACTCTAAG
    TCGGAGGTGC CCGAGCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CCGTGAGAAC GACCTCTCGT TCCCCTGTCC TCTCCCGTCG GGATTCCCCA
    CTGCAGGGCG GTGGACAGCA GAATAGCCAA GCAGGACAGA GAAATTCCAC CAGCAGTATT
    GAGCCCCGGC TTCTTTGGGA GAGAGTGGAA AAGCTGGTCC CCAGGCCAGG CAGTGGCAGC
    TCTTCAGGGT CCAGCAACTC AGGATCCCAG CCTGGCTCCC ATCCTGGGTC TCAGAGCGGC
    TCCGGTGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGTTCTCC ATCGCCACGC
    CAGGAAAGTG CAGCCAAAAA GCCTGACGAT AAGAAAGAAG TATTCCGGCC TCTCAAGCCT
    GCGGGAGAAG TGGACTTGAC TGCGTTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTTCGG
    CCACCTCACA AAGTAACAGA CTACTCATCC TCCAGTGAGG AGTCTGGGAC CACAGATGAG
    GAGGAGGAGG ACGTGGAACA GGAGGGGGCT GATGATTCTA CCTCGGGACC AGAAGACACC
    AGAGCAGCGT CATCTCCGAA CCTGAGCAAC GGTGAAACAG AATCTGTGAA AACAATGATC
    GTTCATGATG ATGTAGAAAG TGAGCCAGCC ATGACCCCGT CCAAGGAGGG CACCCTCATC
    GTCCGCCAGA GTACAGTTGA CCAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCG
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC
    ACCTCTTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG
    GACAAGCTCA CTGCTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGGTTACTAC AGATCTCTCC ATCTAGTGGG
    ACGACAGTGA CTTCCGTGGT GGGATTTTCC TGTGATGGGC TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CTCGGAAAGG CTCAGTGGTC AATGTGAACC CCACGAACAC TAGGCCACAG
    AGTGATACCC CCGAGATTCG TAAATACAAG AAGAGATTTA ACTCAGAGAT CCTGTGTGCT
    GCCTTATGGG GAGTGAACTT GCTGGTTGGT ACAGAGAGCG GTCTGATGCT CTTGGACAGA
    AGTGGCCAAG GGAAAGTATA CCCTCTGATC AGCCGAAGAC GGTTCCAGCA AATGGATGTG
    CTCGAAGGCC TAAATGTCCT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC
    TATTTATCCT GGCTAAGAAA TAAGATTCTT CATAATGATC CAGAGGTTGA GAAGAAGCAG
    GGGTGGACCA CTGTGGGCGA CTTGGAAGGG TGCGTGCACT ATAAAGTCGT AAAATATGAA
    AGAATCAAGT TTCTGGTAAT TGCTTTGAAG AGTTCTGTGG AAGTCTATGC ATGGGCACCG
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AACTGCTACA TAAGCCATTA
    CTGGTGGATC TCACTGTGGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCG
    GGATTCCATG CTGTTGATGT GGATTCAGGC TCAGTCTATG ACATTTATCT ACCAACACAT
    ATTCAGTGTA GCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGGATGGAG
    CTGCTGGTGT GCTACGAAGA CGAGGGGGTT TACGTTAACA CGTACGGAAG GATCACCAAG
    GACGTGGTTC TGCAGTGGGG AGAGATGCCG ACATCTGTAG CATATATCCG GTCGAATCAG
    ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGATCTG TGGAAACTGG TCACTTGGAT
    GGTGTATTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG CAATGACAAG
    GTGTTCTTTG CCTCCGTCCG CTCTGGTGGC AGCAGCCAGG TCTATTTCAT GACCTTAGGC
    AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176493.1
    CDS319..4302
    Translation

    Target ORF information:

    RefSeq Version XM_017321004.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GTGGTCCCAC CTGGCATCTC TCAAGAACAA TGTCTCCCCT
    GTCTCGAGAT CCCATTCCTT CAGTGACCCT TCTCCCAAAT TCGCACACCA CCATCTCCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGGGCTCA GTCAGAGCTC TGACTCTAAG
    TCGGAGGTGC CCGAGCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CCGTGAGAAC GACCTCTCGT TCCCCTGTCC TCTCCCGTCG GGATTCCCCA
    CTGCAGGGCG GTGGACAGCA GAATAGCCAA GCAGGACAGA GAAATTCCAC CAGCAGTATT
    GAGCCCCGGC TTCTTTGGGA GAGAGTGGAA AAGCTGGTCC CCAGGCCAGG CAGTGGCAGC
    TCTTCAGGGT CCAGCAACTC AGGATCCCAG CCTGGCTCCC ATCCTGGGTC TCAGAGCGGC
    TCCGGTGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGTTCTCC ATCGCCACGC
    CAGGAAAGTG CAGCCAAAAA GCCTGACGAT AAGAAAGAAG TATTCCGGCC TCTCAAGCCT
    GCGGGAGAAG TGGACTTGAC TGCGTTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTTCGG
    CCACCTCACA AAGTAACAGA CTACTCATCC TCCAGTGAGG AGTCTGGGAC CACAGATGAG
    GAGGAGGAGG ACGTGGAACA GGAGGGGGCT GATGATTCTA CCTCGGGACC AGAAGACACC
    AGAGCAGCGT CATCTCCGAA CCTGAGCAAC GGTGAAACAG AATCTGTGAA AACAATGATC
    GTTCATGATG ATGTAGAAAG TGAGCCAGCC ATGACCCCGT CCAAGGAGGG CACCCTCATC
    GTCCGCCAGA GTACAGTTGA CCAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCG
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC
    ACCTCTTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG
    GACAAGCTCA CTGCTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGGTTACTAC AGATCTCTCC ATCTAGTGGG
    ACGACAGTGA CTTCCGTGGT GGGATTTTCC TGTGATGGGC TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CTCGGAAAGG CTCAGTGGTC AATGTGAACC CCACGAACAC TAGGCCACAG
    AGTGATACCC CCGAGATTCG TAAATACAAG AAGAGATTTA ACTCAGAGAT CCTGTGTGCT
    GCCTTATGGG GAGTGAACTT GCTGGTTGGT ACAGAGAGCG GTCTGATGCT CTTGGACAGA
    AGTGGCCAAG GGAAAGTATA CCCTCTGATC AGCCGAAGAC GGTTCCAGCA AATGGATGTG
    CTCGAAGGCC TAAATGTCCT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC
    TATTTATCCT GGCTAAGAAA TAAGATTCTT CATAATGATC CAGAGGTTGA GAAGAAGCAG
    GGGTGGACCA CTGTGGGCGA CTTGGAAGGG TGCGTGCACT ATAAAGTCGT AAAATATGAA
    AGAATCAAGT TTCTGGTAAT TGCTTTGAAG AGTTCTGTGG AAGTCTATGC ATGGGCACCG
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AACTGCTACA TAAGCCATTA
    CTGGTGGATC TCACTGTGGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCG
    GGATTCCATG CTGTTGATGT GGATTCAGGC TCAGTCTATG ACATTTATCT ACCAACACAT
    ATTCAGTGTA GCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGGATGGAG
    CTGCTGGTGT GCTACGAAGA CGAGGGGGTT TACGTTAACA CGTACGGAAG GATCACCAAG
    GACGTGGTTC TGCAGTGGGG AGAGATGCCG ACATCTGTAG CATATATCCG GTCGAATCAG
    ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGATCTG TGGAAACTGG TCACTTGGAT
    GGTGTATTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG CAATGACAAG
    GTGTTCTTTG CCTCCGTCCG CTCTGGTGGC AGCAGCCAGG TCTATTTCAT GACCTTAGGC
    AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77156
    Clone ID Related Accession (Same CDS sequence) XM_017321004.1 , XM_017321004.2
    Accession Version XM_017321004.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GTGGTCCCAC CTGGCATCTC TCAAGAACAA TGTCTCCCCT
    GTCTCGAGAT CCCATTCCTT CAGTGACCCT TCTCCCAAAT TCGCACACCA CCATCTCCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGGGCTCA GTCAGAGCTC TGACTCTAAG
    TCGGAGGTGC CCGAGCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CCGTGAGAAC GACCTCTCGT TCCCCTGTCC TCTCCCGTCG GGATTCCCCA
    CTGCAGGGCG GTGGACAGCA GAATAGCCAA GCAGGACAGA GAAATTCCAC CAGCAGTATT
    GAGCCCCGGC TTCTTTGGGA GAGAGTGGAA AAGCTGGTCC CCAGGCCAGG CAGTGGCAGC
    TCTTCAGGGT CCAGCAACTC AGGATCCCAG CCTGGCTCCC ATCCTGGGTC TCAGAGCGGC
    TCCGGTGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGTTCTCC ATCGCCACGC
    CAGGAAAGTG CAGCCAAAAA GCCTGACGAT AAGAAAGAAG TATTCCGGCC TCTCAAGCCT
    GCGGGAGAAG TGGACTTGAC TGCGTTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTTCGG
    CCACCTCACA AAGTAACAGA CTACTCATCC TCCAGTGAGG AGTCTGGGAC CACAGATGAG
    GAGGAGGAGG ACGTGGAACA GGAGGGGGCT GATGATTCTA CCTCGGGACC AGAAGACACC
    AGAGCAGCGT CATCTCCGAA CCTGAGCAAC GGTGAAACAG AATCTGTGAA AACAATGATC
    GTTCATGATG ATGTAGAAAG TGAGCCAGCC ATGACCCCGT CCAAGGAGGG CACCCTCATC
    GTCCGCCAGA GTACAGTTGA CCAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCG
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC
    ACCTCTTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG
    GACAAGCTCA CTGCTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGGTTACTAC AGATCTCTCC ATCTAGTGGG
    ACGACAGTGA CTTCCGTGGT GGGATTTTCC TGTGATGGGC TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CTCGGAAAGG CTCAGTGGTC AATGTGAACC CCACGAACAC TAGGCCACAG
    AGTGATACCC CCGAGATTCG TAAATACAAG AAGAGATTTA ACTCAGAGAT CCTGTGTGCT
    GCCTTATGGG GAGTGAACTT GCTGGTTGGT ACAGAGAGCG GTCTGATGCT CTTGGACAGA
    AGTGGCCAAG GGAAAGTATA CCCTCTGATC AGCCGAAGAC GGTTCCAGCA AATGGATGTG
    CTCGAAGGCC TAAATGTCCT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC
    TATTTATCCT GGCTAAGAAA TAAGATTCTT CATAATGATC CAGAGGTTGA GAAGAAGCAG
    GGGTGGACCA CTGTGGGCGA CTTGGAAGGG TGCGTGCACT ATAAAGTCGT AAAATATGAA
    AGAATCAAGT TTCTGGTAAT TGCTTTGAAG AGTTCTGTGG AAGTCTATGC ATGGGCACCG
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AACTGCTACA TAAGCCATTA
    CTGGTGGATC TCACTGTGGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCG
    GGATTCCATG CTGTTGATGT GGATTCAGGC TCAGTCTATG ACATTTATCT ACCAACACAT
    ATTCAGTGTA GCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGGATGGAG
    CTGCTGGTGT GCTACGAAGA CGAGGGGGTT TACGTTAACA CGTACGGAAG GATCACCAAG
    GACGTGGTTC TGCAGTGGGG AGAGATGCCG ACATCTGTAG CATATATCCG GTCGAATCAG
    ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGATCTG TGGAAACTGG TCACTTGGAT
    GGTGTATTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG CAATGACAAG
    GTGTTCTTTG CCTCCGTCCG CTCTGGTGGC AGCAGCCAGG TCTATTTCAT GACCTTAGGC
    AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176493.1
    CDS329..4312
    Translation

    Target ORF information:

    RefSeq Version XM_017321004.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321004.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GTGGTCCCAC CTGGCATCTC TCAAGAACAA TGTCTCCCCT
    GTCTCGAGAT CCCATTCCTT CAGTGACCCT TCTCCCAAAT TCGCACACCA CCATCTCCGC
    TCTCAGGACC CATGTCCACC TTCCCGCAGT GAGGGGCTCA GTCAGAGCTC TGACTCTAAG
    TCGGAGGTGC CCGAGCCCAC CCAAAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT
    CCAAGGGTTC CCGTGAGAAC GACCTCTCGT TCCCCTGTCC TCTCCCGTCG GGATTCCCCA
    CTGCAGGGCG GTGGACAGCA GAATAGCCAA GCAGGACAGA GAAATTCCAC CAGCAGTATT
    GAGCCCCGGC TTCTTTGGGA GAGAGTGGAA AAGCTGGTCC CCAGGCCAGG CAGTGGCAGC
    TCTTCAGGGT CCAGCAACTC AGGATCCCAG CCTGGCTCCC ATCCTGGGTC TCAGAGCGGC
    TCCGGTGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGTTCTCC ATCGCCACGC
    CAGGAAAGTG CAGCCAAAAA GCCTGACGAT AAGAAAGAAG TATTCCGGCC TCTCAAGCCT
    GCGGGAGAAG TGGACTTGAC TGCGTTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTTCGG
    CCACCTCACA AAGTAACAGA CTACTCATCC TCCAGTGAGG AGTCTGGGAC CACAGATGAG
    GAGGAGGAGG ACGTGGAACA GGAGGGGGCT GATGATTCTA CCTCGGGACC AGAAGACACC
    AGAGCAGCGT CATCTCCGAA CCTGAGCAAC GGTGAAACAG AATCTGTGAA AACAATGATC
    GTTCATGATG ATGTAGAAAG TGAGCCAGCC ATGACCCCGT CCAAGGAGGG CACCCTCATC
    GTCCGCCAGA GTACAGTTGA CCAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCG
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC
    ACCTCTTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG
    GACAAGCTCA CTGCTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGGTTACTAC AGATCTCTCC ATCTAGTGGG
    ACGACAGTGA CTTCCGTGGT GGGATTTTCC TGTGATGGGC TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CTCGGAAAGG CTCAGTGGTC AATGTGAACC CCACGAACAC TAGGCCACAG
    AGTGATACCC CCGAGATTCG TAAATACAAG AAGAGATTTA ACTCAGAGAT CCTGTGTGCT
    GCCTTATGGG GAGTGAACTT GCTGGTTGGT ACAGAGAGCG GTCTGATGCT CTTGGACAGA
    AGTGGCCAAG GGAAAGTATA CCCTCTGATC AGCCGAAGAC GGTTCCAGCA AATGGATGTG
    CTCGAAGGCC TAAATGTCCT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC
    TATTTATCCT GGCTAAGAAA TAAGATTCTT CATAATGATC CAGAGGTTGA GAAGAAGCAG
    GGGTGGACCA CTGTGGGCGA CTTGGAAGGG TGCGTGCACT ATAAAGTCGT AAAATATGAA
    AGAATCAAGT TTCTGGTAAT TGCTTTGAAG AGTTCTGTGG AAGTCTATGC ATGGGCACCG
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AACTGCTACA TAAGCCATTA
    CTGGTGGATC TCACTGTGGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCG
    GGATTCCATG CTGTTGATGT GGATTCAGGC TCAGTCTATG ACATTTATCT ACCAACACAT
    ATTCAGTGTA GCATCAAACC CCATGCAATC ATTATCCTCC CCAACACGGA TGGGATGGAG
    CTGCTGGTGT GCTACGAAGA CGAGGGGGTT TACGTTAACA CGTACGGAAG GATCACCAAG
    GACGTGGTTC TGCAGTGGGG AGAGATGCCG ACATCTGTAG CATATATCCG GTCGAATCAG
    ACAATGGGCT GGGGAGAGAA GGCAATAGAG ATACGATCTG TGGAAACTGG TCACTTGGAT
    GGTGTATTTA TGCACAAAAG GGCTCAGAGA CTAAAGTTCC TGTGTGAACG CAATGACAAG
    GTGTTCTTTG CCTCCGTCCG CTCTGGTGGC AGCAGCCAGG TCTATTTCAT GACCTTAGGC
    AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77157
    Clone ID Related Accession (Same CDS sequence) XM_017321010.1 , XM_017321010.2
    Accession Version XM_017321010.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3951bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GCAGTATTGA GCCCCGGCTT
    CTTTGGGAGA GAGTGGAAAA GCTGGTCCCC AGGCCAGGCA GTGGCAGCTC TTCAGGGTCC
    AGCAACTCAG GATCCCAGCC TGGCTCCCAT CCTGGGTCTC AGAGCGGCTC CGGTGAACGC
    TTCAGAGTGA GATCATCATC CAAGTCTGAA GGTTCTCCAT CGCCACGCCA GGAAAGTGCA
    GCCAAAAAGC CTGACGATAA GAAAGAAGTA TTCCGGCCTC TCAAGCCTGC GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTTCGGC CACCTCACAA AGTAACAGAC
    TACTCATCCT CCAGTGAGGA GTCTGGGACC ACAGATGAGG AGGAGGAGGA CGTGGAACAG
    GAGGGGGCTG ATGATTCTAC CTCGGGACCA GAAGACACCA GAGCAGCGTC ATCTCCGAAC
    CTGAGCAACG GTGAAACAGA ATCTGTGAAA ACAATGATCG TTCATGATGA TGTAGAAAGT
    GAGCCAGCCA TGACCCCGTC CAAGGAGGGC ACCCTCATCG TCCGCCAGAC TCAGTCCGCT
    AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGG
    TTACTACAGA TCTCTCCATC TAGTGGGACG ACAGTGACTT CCGTGGTGGG ATTTTCCTGT
    GATGGGCTGA GACCAGAAGC CATAAGGCAA GATCCTACTC GGAAAGGCTC AGTGGTCAAT
    GTGAACCCCA CGAACACTAG GCCACAGAGT GATACCCCCG AGATTCGTAA ATACAAGAAG
    AGATTTAACT CAGAGATCCT GTGTGCTGCC TTATGGGGAG TGAACTTGCT GGTTGGTACA
    GAGAGCGGTC TGATGCTCTT GGACAGAAGT GGCCAAGGGA AAGTATACCC TCTGATCAGC
    CGAAGACGGT TCCAGCAAAT GGATGTGCTC GAAGGCCTAA ATGTCCTGGT GACAATATCT
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTATCCTGGC TAAGAAATAA GATTCTTCAT
    AATGATCCAG AGGTTGAGAA GAAGCAGGGG TGGACCACTG TGGGCGACTT GGAAGGGTGC
    GTGCACTATA AAGTCGTAAA ATATGAAAGA ATCAAGTTTC TGGTAATTGC TTTGAAGAGT
    TCTGTGGAAG TCTATGCATG GGCACCGAAG CCATATCACA AATTTATGGC CTTTAAGTCA
    TTTGGAGAAC TGCTACATAA GCCATTACTG GTGGATCTCA CTGTGGAGGA AGGCCAGAGG
    TTGAAAGTGA TCTATGGATC CTGTGCGGGA TTCCATGCTG TTGATGTGGA TTCAGGCTCA
    GTCTATGACA TTTATCTACC AACACATATT CAGTGTAGCA TCAAACCCCA TGCAATCATT
    ATCCTCCCCA ACACGGATGG GATGGAGCTG CTGGTGTGCT ACGAAGACGA GGGGGTTTAC
    GTTAACACGT ACGGAAGGAT CACCAAGGAC GTGGTTCTGC AGTGGGGAGA GATGCCGACA
    TCTGTAGCAT ATATCCGGTC GAATCAGACA ATGGGCTGGG GAGAGAAGGC AATAGAGATA
    CGATCTGTGG AAACTGGTCA CTTGGATGGT GTATTTATGC ACAAAAGGGC TCAGAGACTA
    AAGTTCCTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CCGTCCGCTC TGGTGGCAGC
    AGCCAGGTCT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176499.1
    CDS319..4269
    Translation

    Target ORF information:

    RefSeq Version XM_017321010.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321010.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GCAGTATTGA GCCCCGGCTT
    CTTTGGGAGA GAGTGGAAAA GCTGGTCCCC AGGCCAGGCA GTGGCAGCTC TTCAGGGTCC
    AGCAACTCAG GATCCCAGCC TGGCTCCCAT CCTGGGTCTC AGAGCGGCTC CGGTGAACGC
    TTCAGAGTGA GATCATCATC CAAGTCTGAA GGTTCTCCAT CGCCACGCCA GGAAAGTGCA
    GCCAAAAAGC CTGACGATAA GAAAGAAGTA TTCCGGCCTC TCAAGCCTGC GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTTCGGC CACCTCACAA AGTAACAGAC
    TACTCATCCT CCAGTGAGGA GTCTGGGACC ACAGATGAGG AGGAGGAGGA CGTGGAACAG
    GAGGGGGCTG ATGATTCTAC CTCGGGACCA GAAGACACCA GAGCAGCGTC ATCTCCGAAC
    CTGAGCAACG GTGAAACAGA ATCTGTGAAA ACAATGATCG TTCATGATGA TGTAGAAAGT
    GAGCCAGCCA TGACCCCGTC CAAGGAGGGC ACCCTCATCG TCCGCCAGAC TCAGTCCGCT
    AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGG
    TTACTACAGA TCTCTCCATC TAGTGGGACG ACAGTGACTT CCGTGGTGGG ATTTTCCTGT
    GATGGGCTGA GACCAGAAGC CATAAGGCAA GATCCTACTC GGAAAGGCTC AGTGGTCAAT
    GTGAACCCCA CGAACACTAG GCCACAGAGT GATACCCCCG AGATTCGTAA ATACAAGAAG
    AGATTTAACT CAGAGATCCT GTGTGCTGCC TTATGGGGAG TGAACTTGCT GGTTGGTACA
    GAGAGCGGTC TGATGCTCTT GGACAGAAGT GGCCAAGGGA AAGTATACCC TCTGATCAGC
    CGAAGACGGT TCCAGCAAAT GGATGTGCTC GAAGGCCTAA ATGTCCTGGT GACAATATCT
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTATCCTGGC TAAGAAATAA GATTCTTCAT
    AATGATCCAG AGGTTGAGAA GAAGCAGGGG TGGACCACTG TGGGCGACTT GGAAGGGTGC
    GTGCACTATA AAGTCGTAAA ATATGAAAGA ATCAAGTTTC TGGTAATTGC TTTGAAGAGT
    TCTGTGGAAG TCTATGCATG GGCACCGAAG CCATATCACA AATTTATGGC CTTTAAGTCA
    TTTGGAGAAC TGCTACATAA GCCATTACTG GTGGATCTCA CTGTGGAGGA AGGCCAGAGG
    TTGAAAGTGA TCTATGGATC CTGTGCGGGA TTCCATGCTG TTGATGTGGA TTCAGGCTCA
    GTCTATGACA TTTATCTACC AACACATATT CAGTGTAGCA TCAAACCCCA TGCAATCATT
    ATCCTCCCCA ACACGGATGG GATGGAGCTG CTGGTGTGCT ACGAAGACGA GGGGGTTTAC
    GTTAACACGT ACGGAAGGAT CACCAAGGAC GTGGTTCTGC AGTGGGGAGA GATGCCGACA
    TCTGTAGCAT ATATCCGGTC GAATCAGACA ATGGGCTGGG GAGAGAAGGC AATAGAGATA
    CGATCTGTGG AAACTGGTCA CTTGGATGGT GTATTTATGC ACAAAAGGGC TCAGAGACTA
    AAGTTCCTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CCGTCCGCTC TGGTGGCAGC
    AGCCAGGTCT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77157
    Clone ID Related Accession (Same CDS sequence) XM_017321010.1 , XM_017321010.2
    Accession Version XM_017321010.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3951bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GCAGTATTGA GCCCCGGCTT
    CTTTGGGAGA GAGTGGAAAA GCTGGTCCCC AGGCCAGGCA GTGGCAGCTC TTCAGGGTCC
    AGCAACTCAG GATCCCAGCC TGGCTCCCAT CCTGGGTCTC AGAGCGGCTC CGGTGAACGC
    TTCAGAGTGA GATCATCATC CAAGTCTGAA GGTTCTCCAT CGCCACGCCA GGAAAGTGCA
    GCCAAAAAGC CTGACGATAA GAAAGAAGTA TTCCGGCCTC TCAAGCCTGC GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTTCGGC CACCTCACAA AGTAACAGAC
    TACTCATCCT CCAGTGAGGA GTCTGGGACC ACAGATGAGG AGGAGGAGGA CGTGGAACAG
    GAGGGGGCTG ATGATTCTAC CTCGGGACCA GAAGACACCA GAGCAGCGTC ATCTCCGAAC
    CTGAGCAACG GTGAAACAGA ATCTGTGAAA ACAATGATCG TTCATGATGA TGTAGAAAGT
    GAGCCAGCCA TGACCCCGTC CAAGGAGGGC ACCCTCATCG TCCGCCAGAC TCAGTCCGCT
    AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGG
    TTACTACAGA TCTCTCCATC TAGTGGGACG ACAGTGACTT CCGTGGTGGG ATTTTCCTGT
    GATGGGCTGA GACCAGAAGC CATAAGGCAA GATCCTACTC GGAAAGGCTC AGTGGTCAAT
    GTGAACCCCA CGAACACTAG GCCACAGAGT GATACCCCCG AGATTCGTAA ATACAAGAAG
    AGATTTAACT CAGAGATCCT GTGTGCTGCC TTATGGGGAG TGAACTTGCT GGTTGGTACA
    GAGAGCGGTC TGATGCTCTT GGACAGAAGT GGCCAAGGGA AAGTATACCC TCTGATCAGC
    CGAAGACGGT TCCAGCAAAT GGATGTGCTC GAAGGCCTAA ATGTCCTGGT GACAATATCT
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTATCCTGGC TAAGAAATAA GATTCTTCAT
    AATGATCCAG AGGTTGAGAA GAAGCAGGGG TGGACCACTG TGGGCGACTT GGAAGGGTGC
    GTGCACTATA AAGTCGTAAA ATATGAAAGA ATCAAGTTTC TGGTAATTGC TTTGAAGAGT
    TCTGTGGAAG TCTATGCATG GGCACCGAAG CCATATCACA AATTTATGGC CTTTAAGTCA
    TTTGGAGAAC TGCTACATAA GCCATTACTG GTGGATCTCA CTGTGGAGGA AGGCCAGAGG
    TTGAAAGTGA TCTATGGATC CTGTGCGGGA TTCCATGCTG TTGATGTGGA TTCAGGCTCA
    GTCTATGACA TTTATCTACC AACACATATT CAGTGTAGCA TCAAACCCCA TGCAATCATT
    ATCCTCCCCA ACACGGATGG GATGGAGCTG CTGGTGTGCT ACGAAGACGA GGGGGTTTAC
    GTTAACACGT ACGGAAGGAT CACCAAGGAC GTGGTTCTGC AGTGGGGAGA GATGCCGACA
    TCTGTAGCAT ATATCCGGTC GAATCAGACA ATGGGCTGGG GAGAGAAGGC AATAGAGATA
    CGATCTGTGG AAACTGGTCA CTTGGATGGT GTATTTATGC ACAAAAGGGC TCAGAGACTA
    AAGTTCCTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CCGTCCGCTC TGGTGGCAGC
    AGCCAGGTCT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176499.1
    CDS329..4279
    Translation

    Target ORF information:

    RefSeq Version XM_017321010.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321010.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GCAGTATTGA GCCCCGGCTT
    CTTTGGGAGA GAGTGGAAAA GCTGGTCCCC AGGCCAGGCA GTGGCAGCTC TTCAGGGTCC
    AGCAACTCAG GATCCCAGCC TGGCTCCCAT CCTGGGTCTC AGAGCGGCTC CGGTGAACGC
    TTCAGAGTGA GATCATCATC CAAGTCTGAA GGTTCTCCAT CGCCACGCCA GGAAAGTGCA
    GCCAAAAAGC CTGACGATAA GAAAGAAGTA TTCCGGCCTC TCAAGCCTGC GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTTCGGC CACCTCACAA AGTAACAGAC
    TACTCATCCT CCAGTGAGGA GTCTGGGACC ACAGATGAGG AGGAGGAGGA CGTGGAACAG
    GAGGGGGCTG ATGATTCTAC CTCGGGACCA GAAGACACCA GAGCAGCGTC ATCTCCGAAC
    CTGAGCAACG GTGAAACAGA ATCTGTGAAA ACAATGATCG TTCATGATGA TGTAGAAAGT
    GAGCCAGCCA TGACCCCGTC CAAGGAGGGC ACCCTCATCG TCCGCCAGAC TCAGTCCGCT
    AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGG
    TTACTACAGA TCTCTCCATC TAGTGGGACG ACAGTGACTT CCGTGGTGGG ATTTTCCTGT
    GATGGGCTGA GACCAGAAGC CATAAGGCAA GATCCTACTC GGAAAGGCTC AGTGGTCAAT
    GTGAACCCCA CGAACACTAG GCCACAGAGT GATACCCCCG AGATTCGTAA ATACAAGAAG
    AGATTTAACT CAGAGATCCT GTGTGCTGCC TTATGGGGAG TGAACTTGCT GGTTGGTACA
    GAGAGCGGTC TGATGCTCTT GGACAGAAGT GGCCAAGGGA AAGTATACCC TCTGATCAGC
    CGAAGACGGT TCCAGCAAAT GGATGTGCTC GAAGGCCTAA ATGTCCTGGT GACAATATCT
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTATCCTGGC TAAGAAATAA GATTCTTCAT
    AATGATCCAG AGGTTGAGAA GAAGCAGGGG TGGACCACTG TGGGCGACTT GGAAGGGTGC
    GTGCACTATA AAGTCGTAAA ATATGAAAGA ATCAAGTTTC TGGTAATTGC TTTGAAGAGT
    TCTGTGGAAG TCTATGCATG GGCACCGAAG CCATATCACA AATTTATGGC CTTTAAGTCA
    TTTGGAGAAC TGCTACATAA GCCATTACTG GTGGATCTCA CTGTGGAGGA AGGCCAGAGG
    TTGAAAGTGA TCTATGGATC CTGTGCGGGA TTCCATGCTG TTGATGTGGA TTCAGGCTCA
    GTCTATGACA TTTATCTACC AACACATATT CAGTGTAGCA TCAAACCCCA TGCAATCATT
    ATCCTCCCCA ACACGGATGG GATGGAGCTG CTGGTGTGCT ACGAAGACGA GGGGGTTTAC
    GTTAACACGT ACGGAAGGAT CACCAAGGAC GTGGTTCTGC AGTGGGGAGA GATGCCGACA
    TCTGTAGCAT ATATCCGGTC GAATCAGACA ATGGGCTGGG GAGAGAAGGC AATAGAGATA
    CGATCTGTGG AAACTGGTCA CTTGGATGGT GTATTTATGC ACAAAAGGGC TCAGAGACTA
    AAGTTCCTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CCGTCCGCTC TGGTGGCAGC
    AGCCAGGTCT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77158
    Clone ID Related Accession (Same CDS sequence) XM_017321011.2 , XM_017321011.1
    Accession Version XM_017321011.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTTCCCG TGAGAACGAC CTCTCGTTCC CCTGTCCTCT CCCGTCGGGA TTCCCCACTG
    CAGGGCGGTG GACAGCAGAA TAGCCAAGCA GGACAGAGAA ATTCCACCAG CAGTATTGAG
    CCCCGGCTTC TTTGGGAGAG AGTGGAAAAG CTGGTCCCCA GGCCAGGCAG TGGCAGCTCT
    TCAGGGTCCA GCAACTCAGG ATCCCAGCCT GGCTCCCATC CTGGGTCTCA GAGCGGCTCC
    GGTGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GTTCTCCATC GCCACGCCAG
    GAAAGTGCAG CCAAAAAGCC TGACGATAAG AAAGAAGTAT TCCGGCCTCT CAAGCCTGCG
    GGAGAAGTGG ACTTGACTGC GTTGGCCAAA GAGCTTCGAG CAGTGGAAGA TGTTCGGCCA
    CCTCACAAAG TAACAGACTA CTCATCCTCC AGTGAGGAGT CTGGGACCAC AGATGAGGAG
    GAGGAGGACG TGGAACAGGA GGGGGCTGAT GATTCTACCT CGGGACCAGA AGACACCAGA
    GCAGCGTCAT CTCCGAACCT GAGCAACGGT GAAACAGAAT CTGTGAAAAC AATGATCGTT
    CATGATGATG TAGAAAGTGA GCCAGCCATG ACCCCGTCCA AGGAGGGCAC CCTCATCGTC
    CGCCAGAGTA CAGTTGACCA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCGGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCTTCCTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTCACTG CTAATGAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGG TTACTACAGA TCTCTCCATC TAGTGGGACG
    ACAGTGACTT CCGTGGTGGG ATTTTCCTGT GATGGGCTGA GACCAGAAGC CATAAGGCAA
    GATCCTACTC GGAAAGGCTC AGTGGTCAAT GTGAACCCCA CGAACACTAG GCCACAGAGT
    GATACCCCCG AGATTCGTAA ATACAAGAAG AGATTTAACT CAGAGATCCT GTGTGCTGCC
    TTATGGGGAG TGAACTTGCT GGTTGGTACA GAGAGCGGTC TGATGCTCTT GGACAGAAGT
    GGCCAAGGGA AAGTATACCC TCTGATCAGC CGAAGACGGT TCCAGCAAAT GGATGTGCTC
    GAAGGCCTAA ATGTCCTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT
    TTATCCTGGC TAAGAAATAA GATTCTTCAT AATGATCCAG AGGTTGAGAA GAAGCAGGGG
    TGGACCACTG TGGGCGACTT GGAAGGGTGC GTGCACTATA AAGTCGTAAA ATATGAAAGA
    ATCAAGTTTC TGGTAATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCATG GGCACCGAAG
    CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAC TGCTACATAA GCCATTACTG
    GTGGATCTCA CTGTGGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCGGGA
    TTCCATGCTG TTGATGTGGA TTCAGGCTCA GTCTATGACA TTTATCTACC AACACATATT
    CAGTGTAGCA TCAAACCCCA TGCAATCATT ATCCTCCCCA ACACGGATGG GATGGAGCTG
    CTGGTGTGCT ACGAAGACGA GGGGGTTTAC GTTAACACGT ACGGAAGGAT CACCAAGGAC
    GTGGTTCTGC AGTGGGGAGA GATGCCGACA TCTGTAGCAT ATATCCGGTC GAATCAGACA
    ATGGGCTGGG GAGAGAAGGC AATAGAGATA CGATCTGTGG AAACTGGTCA CTTGGATGGT
    GTATTTATGC ACAAAAGGGC TCAGAGACTA AAGTTCCTGT GTGAACGCAA TGACAAGGTG
    TTCTTTGCCT CCGTCCGCTC TGGTGGCAGC AGCCAGGTCT ATTTCATGAC CTTAGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176500.1
    CDS329..4249
    Translation

    Target ORF information:

    RefSeq Version XM_017321011.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321011.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTTCCCG TGAGAACGAC CTCTCGTTCC CCTGTCCTCT CCCGTCGGGA TTCCCCACTG
    CAGGGCGGTG GACAGCAGAA TAGCCAAGCA GGACAGAGAA ATTCCACCAG CAGTATTGAG
    CCCCGGCTTC TTTGGGAGAG AGTGGAAAAG CTGGTCCCCA GGCCAGGCAG TGGCAGCTCT
    TCAGGGTCCA GCAACTCAGG ATCCCAGCCT GGCTCCCATC CTGGGTCTCA GAGCGGCTCC
    GGTGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GTTCTCCATC GCCACGCCAG
    GAAAGTGCAG CCAAAAAGCC TGACGATAAG AAAGAAGTAT TCCGGCCTCT CAAGCCTGCG
    GGAGAAGTGG ACTTGACTGC GTTGGCCAAA GAGCTTCGAG CAGTGGAAGA TGTTCGGCCA
    CCTCACAAAG TAACAGACTA CTCATCCTCC AGTGAGGAGT CTGGGACCAC AGATGAGGAG
    GAGGAGGACG TGGAACAGGA GGGGGCTGAT GATTCTACCT CGGGACCAGA AGACACCAGA
    GCAGCGTCAT CTCCGAACCT GAGCAACGGT GAAACAGAAT CTGTGAAAAC AATGATCGTT
    CATGATGATG TAGAAAGTGA GCCAGCCATG ACCCCGTCCA AGGAGGGCAC CCTCATCGTC
    CGCCAGAGTA CAGTTGACCA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCGGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCTTCCTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTCACTG CTAATGAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGG TTACTACAGA TCTCTCCATC TAGTGGGACG
    ACAGTGACTT CCGTGGTGGG ATTTTCCTGT GATGGGCTGA GACCAGAAGC CATAAGGCAA
    GATCCTACTC GGAAAGGCTC AGTGGTCAAT GTGAACCCCA CGAACACTAG GCCACAGAGT
    GATACCCCCG AGATTCGTAA ATACAAGAAG AGATTTAACT CAGAGATCCT GTGTGCTGCC
    TTATGGGGAG TGAACTTGCT GGTTGGTACA GAGAGCGGTC TGATGCTCTT GGACAGAAGT
    GGCCAAGGGA AAGTATACCC TCTGATCAGC CGAAGACGGT TCCAGCAAAT GGATGTGCTC
    GAAGGCCTAA ATGTCCTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT
    TTATCCTGGC TAAGAAATAA GATTCTTCAT AATGATCCAG AGGTTGAGAA GAAGCAGGGG
    TGGACCACTG TGGGCGACTT GGAAGGGTGC GTGCACTATA AAGTCGTAAA ATATGAAAGA
    ATCAAGTTTC TGGTAATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCATG GGCACCGAAG
    CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAC TGCTACATAA GCCATTACTG
    GTGGATCTCA CTGTGGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCGGGA
    TTCCATGCTG TTGATGTGGA TTCAGGCTCA GTCTATGACA TTTATCTACC AACACATATT
    CAGTGTAGCA TCAAACCCCA TGCAATCATT ATCCTCCCCA ACACGGATGG GATGGAGCTG
    CTGGTGTGCT ACGAAGACGA GGGGGTTTAC GTTAACACGT ACGGAAGGAT CACCAAGGAC
    GTGGTTCTGC AGTGGGGAGA GATGCCGACA TCTGTAGCAT ATATCCGGTC GAATCAGACA
    ATGGGCTGGG GAGAGAAGGC AATAGAGATA CGATCTGTGG AAACTGGTCA CTTGGATGGT
    GTATTTATGC ACAAAAGGGC TCAGAGACTA AAGTTCCTGT GTGAACGCAA TGACAAGGTG
    TTCTTTGCCT CCGTCCGCTC TGGTGGCAGC AGCCAGGTCT ATTTCATGAC CTTAGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77158
    Clone ID Related Accession (Same CDS sequence) XM_017321011.2 , XM_017321011.1
    Accession Version XM_017321011.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTTCCCG TGAGAACGAC CTCTCGTTCC CCTGTCCTCT CCCGTCGGGA TTCCCCACTG
    CAGGGCGGTG GACAGCAGAA TAGCCAAGCA GGACAGAGAA ATTCCACCAG CAGTATTGAG
    CCCCGGCTTC TTTGGGAGAG AGTGGAAAAG CTGGTCCCCA GGCCAGGCAG TGGCAGCTCT
    TCAGGGTCCA GCAACTCAGG ATCCCAGCCT GGCTCCCATC CTGGGTCTCA GAGCGGCTCC
    GGTGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GTTCTCCATC GCCACGCCAG
    GAAAGTGCAG CCAAAAAGCC TGACGATAAG AAAGAAGTAT TCCGGCCTCT CAAGCCTGCG
    GGAGAAGTGG ACTTGACTGC GTTGGCCAAA GAGCTTCGAG CAGTGGAAGA TGTTCGGCCA
    CCTCACAAAG TAACAGACTA CTCATCCTCC AGTGAGGAGT CTGGGACCAC AGATGAGGAG
    GAGGAGGACG TGGAACAGGA GGGGGCTGAT GATTCTACCT CGGGACCAGA AGACACCAGA
    GCAGCGTCAT CTCCGAACCT GAGCAACGGT GAAACAGAAT CTGTGAAAAC AATGATCGTT
    CATGATGATG TAGAAAGTGA GCCAGCCATG ACCCCGTCCA AGGAGGGCAC CCTCATCGTC
    CGCCAGAGTA CAGTTGACCA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCGGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCTTCCTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTCACTG CTAATGAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGG TTACTACAGA TCTCTCCATC TAGTGGGACG
    ACAGTGACTT CCGTGGTGGG ATTTTCCTGT GATGGGCTGA GACCAGAAGC CATAAGGCAA
    GATCCTACTC GGAAAGGCTC AGTGGTCAAT GTGAACCCCA CGAACACTAG GCCACAGAGT
    GATACCCCCG AGATTCGTAA ATACAAGAAG AGATTTAACT CAGAGATCCT GTGTGCTGCC
    TTATGGGGAG TGAACTTGCT GGTTGGTACA GAGAGCGGTC TGATGCTCTT GGACAGAAGT
    GGCCAAGGGA AAGTATACCC TCTGATCAGC CGAAGACGGT TCCAGCAAAT GGATGTGCTC
    GAAGGCCTAA ATGTCCTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT
    TTATCCTGGC TAAGAAATAA GATTCTTCAT AATGATCCAG AGGTTGAGAA GAAGCAGGGG
    TGGACCACTG TGGGCGACTT GGAAGGGTGC GTGCACTATA AAGTCGTAAA ATATGAAAGA
    ATCAAGTTTC TGGTAATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCATG GGCACCGAAG
    CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAC TGCTACATAA GCCATTACTG
    GTGGATCTCA CTGTGGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCGGGA
    TTCCATGCTG TTGATGTGGA TTCAGGCTCA GTCTATGACA TTTATCTACC AACACATATT
    CAGTGTAGCA TCAAACCCCA TGCAATCATT ATCCTCCCCA ACACGGATGG GATGGAGCTG
    CTGGTGTGCT ACGAAGACGA GGGGGTTTAC GTTAACACGT ACGGAAGGAT CACCAAGGAC
    GTGGTTCTGC AGTGGGGAGA GATGCCGACA TCTGTAGCAT ATATCCGGTC GAATCAGACA
    ATGGGCTGGG GAGAGAAGGC AATAGAGATA CGATCTGTGG AAACTGGTCA CTTGGATGGT
    GTATTTATGC ACAAAAGGGC TCAGAGACTA AAGTTCCTGT GTGAACGCAA TGACAAGGTG
    TTCTTTGCCT CCGTCCGCTC TGGTGGCAGC AGCCAGGTCT ATTTCATGAC CTTAGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176500.1
    CDS319..4239
    Translation

    Target ORF information:

    RefSeq Version XM_017321011.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321011.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTTCCCG TGAGAACGAC CTCTCGTTCC CCTGTCCTCT CCCGTCGGGA TTCCCCACTG
    CAGGGCGGTG GACAGCAGAA TAGCCAAGCA GGACAGAGAA ATTCCACCAG CAGTATTGAG
    CCCCGGCTTC TTTGGGAGAG AGTGGAAAAG CTGGTCCCCA GGCCAGGCAG TGGCAGCTCT
    TCAGGGTCCA GCAACTCAGG ATCCCAGCCT GGCTCCCATC CTGGGTCTCA GAGCGGCTCC
    GGTGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GTTCTCCATC GCCACGCCAG
    GAAAGTGCAG CCAAAAAGCC TGACGATAAG AAAGAAGTAT TCCGGCCTCT CAAGCCTGCG
    GGAGAAGTGG ACTTGACTGC GTTGGCCAAA GAGCTTCGAG CAGTGGAAGA TGTTCGGCCA
    CCTCACAAAG TAACAGACTA CTCATCCTCC AGTGAGGAGT CTGGGACCAC AGATGAGGAG
    GAGGAGGACG TGGAACAGGA GGGGGCTGAT GATTCTACCT CGGGACCAGA AGACACCAGA
    GCAGCGTCAT CTCCGAACCT GAGCAACGGT GAAACAGAAT CTGTGAAAAC AATGATCGTT
    CATGATGATG TAGAAAGTGA GCCAGCCATG ACCCCGTCCA AGGAGGGCAC CCTCATCGTC
    CGCCAGAGTA CAGTTGACCA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCGGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCTTCCTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTCACTG CTAATGAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGG TTACTACAGA TCTCTCCATC TAGTGGGACG
    ACAGTGACTT CCGTGGTGGG ATTTTCCTGT GATGGGCTGA GACCAGAAGC CATAAGGCAA
    GATCCTACTC GGAAAGGCTC AGTGGTCAAT GTGAACCCCA CGAACACTAG GCCACAGAGT
    GATACCCCCG AGATTCGTAA ATACAAGAAG AGATTTAACT CAGAGATCCT GTGTGCTGCC
    TTATGGGGAG TGAACTTGCT GGTTGGTACA GAGAGCGGTC TGATGCTCTT GGACAGAAGT
    GGCCAAGGGA AAGTATACCC TCTGATCAGC CGAAGACGGT TCCAGCAAAT GGATGTGCTC
    GAAGGCCTAA ATGTCCTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT
    TTATCCTGGC TAAGAAATAA GATTCTTCAT AATGATCCAG AGGTTGAGAA GAAGCAGGGG
    TGGACCACTG TGGGCGACTT GGAAGGGTGC GTGCACTATA AAGTCGTAAA ATATGAAAGA
    ATCAAGTTTC TGGTAATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCATG GGCACCGAAG
    CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAC TGCTACATAA GCCATTACTG
    GTGGATCTCA CTGTGGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCGGGA
    TTCCATGCTG TTGATGTGGA TTCAGGCTCA GTCTATGACA TTTATCTACC AACACATATT
    CAGTGTAGCA TCAAACCCCA TGCAATCATT ATCCTCCCCA ACACGGATGG GATGGAGCTG
    CTGGTGTGCT ACGAAGACGA GGGGGTTTAC GTTAACACGT ACGGAAGGAT CACCAAGGAC
    GTGGTTCTGC AGTGGGGAGA GATGCCGACA TCTGTAGCAT ATATCCGGTC GAATCAGACA
    ATGGGCTGGG GAGAGAAGGC AATAGAGATA CGATCTGTGG AAACTGGTCA CTTGGATGGT
    GTATTTATGC ACAAAAGGGC TCAGAGACTA AAGTTCCTGT GTGAACGCAA TGACAAGGTG
    TTCTTTGCCT CCGTCCGCTC TGGTGGCAGC AGCCAGGTCT ATTTCATGAC CTTAGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77159
    Clone ID Related Accession (Same CDS sequence) XM_017321013.1 , XM_017321013.2
    Accession Version XM_017321013.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT ACAGTGGTCC
    CACCTGGCAT CTCTCAAGAA CAATGTCTCC CCTGTCTCGA GATCCCATTC CTTCAGTGAC
    CCTTCTCCCA AATTCGCACA CCACCATCTC CGCTCTCAGG ACCCATGTCC ACCTTCCCGC
    AGTGAGGGGC TCAGTCAGAG CTCTGACTCT AAGTCGGAGG TGCCCGAGCC CACCCAAAAG
    GCTTGGTCTA GATCAGACAG TGACGAGGTG CCTCCAAGGG TTCCCGTGAG AACGACCTCT
    CGTTCCCCTG TCCTCTCCCG TCGGGATTCC CCACTGCAGG GCGGTGGACA GCAGAATAGC
    CAAGCAGGAC AGAGAAATTC CACCAGCAGT ATTGAGCCCC GGCTTCTTTG GGAGAGAGTG
    GAAAAGCTGG TCCCCAGGCC AGGCAGTGGC AGCTCTTCAG GGTCCAGCAA CTCAGGATCC
    CAGCCTGGCT CCCATCCTGG GTCTCAGAGC GGCTCCGGTG AACGCTTCAG AGTGAGATCA
    TCATCCAAGT CTGAAGGTTC TCCATCGCCA CGCCAGGAAA GTGCAGCCAA AAAGCCTGAC
    GATAAGAAAG AAGTATTCCG GCCTCTCAAG CCTGCGGGAG AAGTGGACTT GACTGCGTTG
    GCCAAAGAGC TTCGAGCAGT GGAAGATGTT CGGCCACCTC ACAAAGTAAC AGACTACTCA
    TCCTCCAGTG AGGAGTCTGG GACCACAGAT GAGGAGGAGG AGGACGTGGA ACAGGAGGGG
    GCTGATGATT CTACCTCGGG ACCAGAAGAC ACCAGAGCAG CGTCATCTCC GAACCTGAGC
    AACGGTGAAA CAGAATCTGT GAAAACAATG ATCGTTCATG ATGATGTAGA AAGTGAGCCA
    GCCATGACCC CGTCCAAGGA GGGCACCCTC ATCGTCCGCC AGAGTACAGT TGACCAAAAG
    CGTGCCAGCC ATCATGAGAG CAATGGCTTT GCGGGTCGCA TTCACCTCTT GCCAGATCTC
    TTACAGCAAA GCCATTCCTC CTCCACTTCC TCCACCTCTT CCTCCCCATC CTCCAGCCAG
    CCGACACCCA CCATGTCCCC ACAGACACCC CAGGACAAGC TCACTGCTAA TGAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGGTTAC TACAGATCTC TCCATCTAGT GGGACGACAG TGACTTCCGT GGTGGGATTT
    TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG
    GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC
    AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA CTTGCTGGTT
    GGTACAGAGA GCGGTCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT ATACCCTCTG
    ATCAGCCGAA GACGGTTCCA GCAAATGGAT GTGCTCGAAG GCCTAAATGT CCTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTAT CCTGGCTAAG AAATAAGATT
    CTTCATAATG ATCCAGAGGT TGAGAAGAAG CAGGGGTGGA CCACTGTGGG CGACTTGGAA
    GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATTGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAACTGCT ACATAAGCCA TTACTGGTGG ATCTCACTGT GGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCGGGATTCC ATGCTGTTGA TGTGGATTCA
    GGCTCAGTCT ATGACATTTA TCTACCAACA CATATTCAGT GTAGCATCAA ACCCCATGCA
    ATCATTATCC TCCCCAACAC GGATGGGATG GAGCTGCTGG TGTGCTACGA AGACGAGGGG
    GTTTACGTTA ACACGTACGG AAGGATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG
    CCGACATCTG TAGCATATAT CCGGTCGAAT CAGACAATGG GCTGGGGAGA GAAGGCAATA
    GAGATACGAT CTGTGGAAAC TGGTCACTTG GATGGTGTAT TTATGCACAA AAGGGCTCAG
    AGACTAAAGT TCCTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCCGT CCGCTCTGGT
    GGCAGCAGCC AGGTCTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176502.1
    CDS319..4215
    Translation

    Target ORF information:

    RefSeq Version XM_017321013.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321013.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT ACAGTGGTCC
    CACCTGGCAT CTCTCAAGAA CAATGTCTCC CCTGTCTCGA GATCCCATTC CTTCAGTGAC
    CCTTCTCCCA AATTCGCACA CCACCATCTC CGCTCTCAGG ACCCATGTCC ACCTTCCCGC
    AGTGAGGGGC TCAGTCAGAG CTCTGACTCT AAGTCGGAGG TGCCCGAGCC CACCCAAAAG
    GCTTGGTCTA GATCAGACAG TGACGAGGTG CCTCCAAGGG TTCCCGTGAG AACGACCTCT
    CGTTCCCCTG TCCTCTCCCG TCGGGATTCC CCACTGCAGG GCGGTGGACA GCAGAATAGC
    CAAGCAGGAC AGAGAAATTC CACCAGCAGT ATTGAGCCCC GGCTTCTTTG GGAGAGAGTG
    GAAAAGCTGG TCCCCAGGCC AGGCAGTGGC AGCTCTTCAG GGTCCAGCAA CTCAGGATCC
    CAGCCTGGCT CCCATCCTGG GTCTCAGAGC GGCTCCGGTG AACGCTTCAG AGTGAGATCA
    TCATCCAAGT CTGAAGGTTC TCCATCGCCA CGCCAGGAAA GTGCAGCCAA AAAGCCTGAC
    GATAAGAAAG AAGTATTCCG GCCTCTCAAG CCTGCGGGAG AAGTGGACTT GACTGCGTTG
    GCCAAAGAGC TTCGAGCAGT GGAAGATGTT CGGCCACCTC ACAAAGTAAC AGACTACTCA
    TCCTCCAGTG AGGAGTCTGG GACCACAGAT GAGGAGGAGG AGGACGTGGA ACAGGAGGGG
    GCTGATGATT CTACCTCGGG ACCAGAAGAC ACCAGAGCAG CGTCATCTCC GAACCTGAGC
    AACGGTGAAA CAGAATCTGT GAAAACAATG ATCGTTCATG ATGATGTAGA AAGTGAGCCA
    GCCATGACCC CGTCCAAGGA GGGCACCCTC ATCGTCCGCC AGAGTACAGT TGACCAAAAG
    CGTGCCAGCC ATCATGAGAG CAATGGCTTT GCGGGTCGCA TTCACCTCTT GCCAGATCTC
    TTACAGCAAA GCCATTCCTC CTCCACTTCC TCCACCTCTT CCTCCCCATC CTCCAGCCAG
    CCGACACCCA CCATGTCCCC ACAGACACCC CAGGACAAGC TCACTGCTAA TGAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGGTTAC TACAGATCTC TCCATCTAGT GGGACGACAG TGACTTCCGT GGTGGGATTT
    TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG
    GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC
    AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA CTTGCTGGTT
    GGTACAGAGA GCGGTCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT ATACCCTCTG
    ATCAGCCGAA GACGGTTCCA GCAAATGGAT GTGCTCGAAG GCCTAAATGT CCTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTAT CCTGGCTAAG AAATAAGATT
    CTTCATAATG ATCCAGAGGT TGAGAAGAAG CAGGGGTGGA CCACTGTGGG CGACTTGGAA
    GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATTGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAACTGCT ACATAAGCCA TTACTGGTGG ATCTCACTGT GGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCGGGATTCC ATGCTGTTGA TGTGGATTCA
    GGCTCAGTCT ATGACATTTA TCTACCAACA CATATTCAGT GTAGCATCAA ACCCCATGCA
    ATCATTATCC TCCCCAACAC GGATGGGATG GAGCTGCTGG TGTGCTACGA AGACGAGGGG
    GTTTACGTTA ACACGTACGG AAGGATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG
    CCGACATCTG TAGCATATAT CCGGTCGAAT CAGACAATGG GCTGGGGAGA GAAGGCAATA
    GAGATACGAT CTGTGGAAAC TGGTCACTTG GATGGTGTAT TTATGCACAA AAGGGCTCAG
    AGACTAAAGT TCCTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCCGT CCGCTCTGGT
    GGCAGCAGCC AGGTCTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77159
    Clone ID Related Accession (Same CDS sequence) XM_017321013.1 , XM_017321013.2
    Accession Version XM_017321013.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321013.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT ACAGTGGTCC
    CACCTGGCAT CTCTCAAGAA CAATGTCTCC CCTGTCTCGA GATCCCATTC CTTCAGTGAC
    CCTTCTCCCA AATTCGCACA CCACCATCTC CGCTCTCAGG ACCCATGTCC ACCTTCCCGC
    AGTGAGGGGC TCAGTCAGAG CTCTGACTCT AAGTCGGAGG TGCCCGAGCC CACCCAAAAG
    GCTTGGTCTA GATCAGACAG TGACGAGGTG CCTCCAAGGG TTCCCGTGAG AACGACCTCT
    CGTTCCCCTG TCCTCTCCCG TCGGGATTCC CCACTGCAGG GCGGTGGACA GCAGAATAGC
    CAAGCAGGAC AGAGAAATTC CACCAGCAGT ATTGAGCCCC GGCTTCTTTG GGAGAGAGTG
    GAAAAGCTGG TCCCCAGGCC AGGCAGTGGC AGCTCTTCAG GGTCCAGCAA CTCAGGATCC
    CAGCCTGGCT CCCATCCTGG GTCTCAGAGC GGCTCCGGTG AACGCTTCAG AGTGAGATCA
    TCATCCAAGT CTGAAGGTTC TCCATCGCCA CGCCAGGAAA GTGCAGCCAA AAAGCCTGAC
    GATAAGAAAG AAGTATTCCG GCCTCTCAAG CCTGCGGGAG AAGTGGACTT GACTGCGTTG
    GCCAAAGAGC TTCGAGCAGT GGAAGATGTT CGGCCACCTC ACAAAGTAAC AGACTACTCA
    TCCTCCAGTG AGGAGTCTGG GACCACAGAT GAGGAGGAGG AGGACGTGGA ACAGGAGGGG
    GCTGATGATT CTACCTCGGG ACCAGAAGAC ACCAGAGCAG CGTCATCTCC GAACCTGAGC
    AACGGTGAAA CAGAATCTGT GAAAACAATG ATCGTTCATG ATGATGTAGA AAGTGAGCCA
    GCCATGACCC CGTCCAAGGA GGGCACCCTC ATCGTCCGCC AGAGTACAGT TGACCAAAAG
    CGTGCCAGCC ATCATGAGAG CAATGGCTTT GCGGGTCGCA TTCACCTCTT GCCAGATCTC
    TTACAGCAAA GCCATTCCTC CTCCACTTCC TCCACCTCTT CCTCCCCATC CTCCAGCCAG
    CCGACACCCA CCATGTCCCC ACAGACACCC CAGGACAAGC TCACTGCTAA TGAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGGTTAC TACAGATCTC TCCATCTAGT GGGACGACAG TGACTTCCGT GGTGGGATTT
    TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG
    GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC
    AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA CTTGCTGGTT
    GGTACAGAGA GCGGTCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT ATACCCTCTG
    ATCAGCCGAA GACGGTTCCA GCAAATGGAT GTGCTCGAAG GCCTAAATGT CCTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTAT CCTGGCTAAG AAATAAGATT
    CTTCATAATG ATCCAGAGGT TGAGAAGAAG CAGGGGTGGA CCACTGTGGG CGACTTGGAA
    GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATTGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAACTGCT ACATAAGCCA TTACTGGTGG ATCTCACTGT GGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCGGGATTCC ATGCTGTTGA TGTGGATTCA
    GGCTCAGTCT ATGACATTTA TCTACCAACA CATATTCAGT GTAGCATCAA ACCCCATGCA
    ATCATTATCC TCCCCAACAC GGATGGGATG GAGCTGCTGG TGTGCTACGA AGACGAGGGG
    GTTTACGTTA ACACGTACGG AAGGATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG
    CCGACATCTG TAGCATATAT CCGGTCGAAT CAGACAATGG GCTGGGGAGA GAAGGCAATA
    GAGATACGAT CTGTGGAAAC TGGTCACTTG GATGGTGTAT TTATGCACAA AAGGGCTCAG
    AGACTAAAGT TCCTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCCGT CCGCTCTGGT
    GGCAGCAGCC AGGTCTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176502.1
    CDS329..4225
    Translation

    Target ORF information:

    RefSeq Version XM_017321013.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321013.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT ACAGTGGTCC
    CACCTGGCAT CTCTCAAGAA CAATGTCTCC CCTGTCTCGA GATCCCATTC CTTCAGTGAC
    CCTTCTCCCA AATTCGCACA CCACCATCTC CGCTCTCAGG ACCCATGTCC ACCTTCCCGC
    AGTGAGGGGC TCAGTCAGAG CTCTGACTCT AAGTCGGAGG TGCCCGAGCC CACCCAAAAG
    GCTTGGTCTA GATCAGACAG TGACGAGGTG CCTCCAAGGG TTCCCGTGAG AACGACCTCT
    CGTTCCCCTG TCCTCTCCCG TCGGGATTCC CCACTGCAGG GCGGTGGACA GCAGAATAGC
    CAAGCAGGAC AGAGAAATTC CACCAGCAGT ATTGAGCCCC GGCTTCTTTG GGAGAGAGTG
    GAAAAGCTGG TCCCCAGGCC AGGCAGTGGC AGCTCTTCAG GGTCCAGCAA CTCAGGATCC
    CAGCCTGGCT CCCATCCTGG GTCTCAGAGC GGCTCCGGTG AACGCTTCAG AGTGAGATCA
    TCATCCAAGT CTGAAGGTTC TCCATCGCCA CGCCAGGAAA GTGCAGCCAA AAAGCCTGAC
    GATAAGAAAG AAGTATTCCG GCCTCTCAAG CCTGCGGGAG AAGTGGACTT GACTGCGTTG
    GCCAAAGAGC TTCGAGCAGT GGAAGATGTT CGGCCACCTC ACAAAGTAAC AGACTACTCA
    TCCTCCAGTG AGGAGTCTGG GACCACAGAT GAGGAGGAGG AGGACGTGGA ACAGGAGGGG
    GCTGATGATT CTACCTCGGG ACCAGAAGAC ACCAGAGCAG CGTCATCTCC GAACCTGAGC
    AACGGTGAAA CAGAATCTGT GAAAACAATG ATCGTTCATG ATGATGTAGA AAGTGAGCCA
    GCCATGACCC CGTCCAAGGA GGGCACCCTC ATCGTCCGCC AGAGTACAGT TGACCAAAAG
    CGTGCCAGCC ATCATGAGAG CAATGGCTTT GCGGGTCGCA TTCACCTCTT GCCAGATCTC
    TTACAGCAAA GCCATTCCTC CTCCACTTCC TCCACCTCTT CCTCCCCATC CTCCAGCCAG
    CCGACACCCA CCATGTCCCC ACAGACACCC CAGGACAAGC TCACTGCTAA TGAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGGTTAC TACAGATCTC TCCATCTAGT GGGACGACAG TGACTTCCGT GGTGGGATTT
    TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG
    GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC
    AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA CTTGCTGGTT
    GGTACAGAGA GCGGTCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT ATACCCTCTG
    ATCAGCCGAA GACGGTTCCA GCAAATGGAT GTGCTCGAAG GCCTAAATGT CCTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTAT CCTGGCTAAG AAATAAGATT
    CTTCATAATG ATCCAGAGGT TGAGAAGAAG CAGGGGTGGA CCACTGTGGG CGACTTGGAA
    GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATTGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAACTGCT ACATAAGCCA TTACTGGTGG ATCTCACTGT GGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCGGGATTCC ATGCTGTTGA TGTGGATTCA
    GGCTCAGTCT ATGACATTTA TCTACCAACA CATATTCAGT GTAGCATCAA ACCCCATGCA
    ATCATTATCC TCCCCAACAC GGATGGGATG GAGCTGCTGG TGTGCTACGA AGACGAGGGG
    GTTTACGTTA ACACGTACGG AAGGATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG
    CCGACATCTG TAGCATATAT CCGGTCGAAT CAGACAATGG GCTGGGGAGA GAAGGCAATA
    GAGATACGAT CTGTGGAAAC TGGTCACTTG GATGGTGTAT TTATGCACAA AAGGGCTCAG
    AGACTAAAGT TCCTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCCGT CCGCTCTGGT
    GGCAGCAGCC AGGTCTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77160
    Clone ID Related Accession (Same CDS sequence) XM_017321016.1
    Accession Version XM_017321016.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTTCCCGTGA GAACGACCTC TCGTTCCCCT
    GTCCTCTCCC GTCGGGATTC CCCACTGCAG GGCGGTGGAC AGCAGAATAG CCAAGCAGGA
    CAGAGAAATT CCACCAGCAG TATTGAGCCC CGGCTTCTTT GGGAGAGAGT GGAAAAGCTG
    GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA GGGTCCAGCA ACTCAGGATC CCAGCCTGGC
    TCCCATCCTG GGTCTCAGAG CGGCTCCGGT GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGTT CTCCATCGCC ACGCCAGGAA AGTGCAGCCA AAAAGCCTGA CGATAAGAAA
    GAAGTATTCC GGCCTCTCAA GCCTGCGGGA GAAGTGGACT TGACTGCGTT GGCCAAAGAG
    CTTCGAGCAG TGGAAGATGT TCGGCCACCT CACAAAGTAA CAGACTACTC ATCCTCCAGT
    GAGGAGTCTG GGACCACAGA TGAGGAGGAG GAGGACGTGG AACAGGAGGG GGCTGATGAT
    TCTACCTCGG GACCAGAAGA CACCAGAGCA GCGTCATCTC CGAACCTGAG CAACGGTGAA
    ACAGAATCTG TGAAAACAAT GATCGTTCAT GATGATGTAG AAAGTGAGCC AGCCATGACC
    CCGTCCAAGG AGGGCACCCT CATCGTCCGC CAGAGTACAG TTGACCAAAA GCGTGCCAGC
    CATCATGAGA GCAATGGCTT TGCGGGTCGC ATTCACCTCT TGCCAGATCT CTTACAGCAA
    AGCCATTCCT CCTCCACTTC CTCCACCTCT TCCTCCCCAT CCTCCAGCCA GCCGACACCC
    ACCATGTCCC CACAGACACC CCAGGACAAG CTCACTGCTA ATGAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGGTTA
    CTACAGATCT CTCCATCTAG TGGGACGACA GTGACTTCCG TGGTGGGATT TTCCTGTGAT
    GGGCTGAGAC CAGAAGCCAT AAGGCAAGAT CCTACTCGGA AAGGCTCAGT GGTCAATGTG
    AACCCCACGA ACACTAGGCC ACAGAGTGAT ACCCCCGAGA TTCGTAAATA CAAGAAGAGA
    TTTAACTCAG AGATCCTGTG TGCTGCCTTA TGGGGAGTGA ACTTGCTGGT TGGTACAGAG
    AGCGGTCTGA TGCTCTTGGA CAGAAGTGGC CAAGGGAAAG TATACCCTCT GATCAGCCGA
    AGACGGTTCC AGCAAATGGA TGTGCTCGAA GGCCTAAATG TCCTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTA TCCTGGCTAA GAAATAAGAT TCTTCATAAT
    GATCCAGAGG TTGAGAAGAA GCAGGGGTGG ACCACTGTGG GCGACTTGGA AGGGTGCGTG
    CACTATAAAG TCGTAAAATA TGAAAGAATC AAGTTTCTGG TAATTGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCATGGGC ACCGAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAACTGC TACATAAGCC ATTACTGGTG GATCTCACTG TGGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCGGGATTC CATGCTGTTG ATGTGGATTC AGGCTCAGTC
    TATGACATTT ATCTACCAAC ACATATTCAG TGTAGCATCA AACCCCATGC AATCATTATC
    CTCCCCAACA CGGATGGGAT GGAGCTGCTG GTGTGCTACG AAGACGAGGG GGTTTACGTT
    AACACGTACG GAAGGATCAC CAAGGACGTG GTTCTGCAGT GGGGAGAGAT GCCGACATCT
    GTAGCATATA TCCGGTCGAA TCAGACAATG GGCTGGGGAG AGAAGGCAAT AGAGATACGA
    TCTGTGGAAA CTGGTCACTT GGATGGTGTA TTTATGCACA AAAGGGCTCA GAGACTAAAG
    TTCCTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCCG TCCGCTCTGG TGGCAGCAGC
    CAGGTCTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176505.1
    CDS319..4146
    Translation

    Target ORF information:

    RefSeq Version XM_017321016.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321016.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTTCCCGTGA GAACGACCTC TCGTTCCCCT
    GTCCTCTCCC GTCGGGATTC CCCACTGCAG GGCGGTGGAC AGCAGAATAG CCAAGCAGGA
    CAGAGAAATT CCACCAGCAG TATTGAGCCC CGGCTTCTTT GGGAGAGAGT GGAAAAGCTG
    GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA GGGTCCAGCA ACTCAGGATC CCAGCCTGGC
    TCCCATCCTG GGTCTCAGAG CGGCTCCGGT GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGTT CTCCATCGCC ACGCCAGGAA AGTGCAGCCA AAAAGCCTGA CGATAAGAAA
    GAAGTATTCC GGCCTCTCAA GCCTGCGGGA GAAGTGGACT TGACTGCGTT GGCCAAAGAG
    CTTCGAGCAG TGGAAGATGT TCGGCCACCT CACAAAGTAA CAGACTACTC ATCCTCCAGT
    GAGGAGTCTG GGACCACAGA TGAGGAGGAG GAGGACGTGG AACAGGAGGG GGCTGATGAT
    TCTACCTCGG GACCAGAAGA CACCAGAGCA GCGTCATCTC CGAACCTGAG CAACGGTGAA
    ACAGAATCTG TGAAAACAAT GATCGTTCAT GATGATGTAG AAAGTGAGCC AGCCATGACC
    CCGTCCAAGG AGGGCACCCT CATCGTCCGC CAGAGTACAG TTGACCAAAA GCGTGCCAGC
    CATCATGAGA GCAATGGCTT TGCGGGTCGC ATTCACCTCT TGCCAGATCT CTTACAGCAA
    AGCCATTCCT CCTCCACTTC CTCCACCTCT TCCTCCCCAT CCTCCAGCCA GCCGACACCC
    ACCATGTCCC CACAGACACC CCAGGACAAG CTCACTGCTA ATGAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGGTTA
    CTACAGATCT CTCCATCTAG TGGGACGACA GTGACTTCCG TGGTGGGATT TTCCTGTGAT
    GGGCTGAGAC CAGAAGCCAT AAGGCAAGAT CCTACTCGGA AAGGCTCAGT GGTCAATGTG
    AACCCCACGA ACACTAGGCC ACAGAGTGAT ACCCCCGAGA TTCGTAAATA CAAGAAGAGA
    TTTAACTCAG AGATCCTGTG TGCTGCCTTA TGGGGAGTGA ACTTGCTGGT TGGTACAGAG
    AGCGGTCTGA TGCTCTTGGA CAGAAGTGGC CAAGGGAAAG TATACCCTCT GATCAGCCGA
    AGACGGTTCC AGCAAATGGA TGTGCTCGAA GGCCTAAATG TCCTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTA TCCTGGCTAA GAAATAAGAT TCTTCATAAT
    GATCCAGAGG TTGAGAAGAA GCAGGGGTGG ACCACTGTGG GCGACTTGGA AGGGTGCGTG
    CACTATAAAG TCGTAAAATA TGAAAGAATC AAGTTTCTGG TAATTGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCATGGGC ACCGAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAACTGC TACATAAGCC ATTACTGGTG GATCTCACTG TGGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCGGGATTC CATGCTGTTG ATGTGGATTC AGGCTCAGTC
    TATGACATTT ATCTACCAAC ACATATTCAG TGTAGCATCA AACCCCATGC AATCATTATC
    CTCCCCAACA CGGATGGGAT GGAGCTGCTG GTGTGCTACG AAGACGAGGG GGTTTACGTT
    AACACGTACG GAAGGATCAC CAAGGACGTG GTTCTGCAGT GGGGAGAGAT GCCGACATCT
    GTAGCATATA TCCGGTCGAA TCAGACAATG GGCTGGGGAG AGAAGGCAAT AGAGATACGA
    TCTGTGGAAA CTGGTCACTT GGATGGTGTA TTTATGCACA AAAGGGCTCA GAGACTAAAG
    TTCCTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCCG TCCGCTCTGG TGGCAGCAGC
    CAGGTCTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77161
    Clone ID Related Accession (Same CDS sequence) XM_017321018.1 , XM_017321018.2
    Accession Version XM_017321018.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3759bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTTCCCGTG AGAACGACCT CTCGTTCCCC TGTCCTCTCC
    CGTCGGGATT CCCCACTGCA GGGCGGTGGA CAGCAGAATA GCCAAGCAGG ACAGAGAAAT
    TCCACCAGCA GTATTGAGCC CCGGCTTCTT TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG
    CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC AACTCAGGAT CCCAGCCTGG CTCCCATCCT
    GGGTCTCAGA GCGGCTCCGG TGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGT
    TCTCCATCGC CACGCCAGGA AAGTGCAGCC AAAAAGCCTG ACGATAAGAA AGAAGTATTC
    CGGCCTCTCA AGCCTGCGGG AGAAGTGGAC TTGACTGCGT TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TTCGGCCACC TCACAAAGTA ACAGACTACT CATCCTCCAG TGAGGAGTCT
    GGGACCACAG ATGAGGAGGA GGAGGACGTG GAACAGGAGG GGGCTGATGA TTCTACCTCG
    GGACCAGAAG ACACCAGAGC AGCGTCATCT CCGAACCTGA GCAACGGTGA AACAGAATCT
    GTGAAAACAA TGATCGTTCA TGATGATGTA GAAAGTGAGC CAGCCATGAC CCCGTCCAAG
    GAGGGCACCC TCATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCGGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC TTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGGTT ACTACAGATC
    TCTCCATCTA GTGGGACGAC AGTGACTTCC GTGGTGGGAT TTTCCTGTGA TGGGCTGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACTCGG AAAGGCTCAG TGGTCAATGT GAACCCCACG
    AACACTAGGC CACAGAGTGA TACCCCCGAG ATTCGTAAAT ACAAGAAGAG ATTTAACTCA
    GAGATCCTGT GTGCTGCCTT ATGGGGAGTG AACTTGCTGG TTGGTACAGA GAGCGGTCTG
    ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA GTATACCCTC TGATCAGCCG AAGACGGTTC
    CAGCAAATGG ATGTGCTCGA AGGCCTAAAT GTCCTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT ATCCTGGCTA AGAAATAAGA TTCTTCATAA TGATCCAGAG
    GTTGAGAAGA AGCAGGGGTG GACCACTGTG GGCGACTTGG AAGGGTGCGT GCACTATAAA
    GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG GTAATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCATGGG CACCGAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAACTG
    CTACATAAGC CATTACTGGT GGATCTCACT GTGGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCGGGATT CCATGCTGTT GATGTGGATT CAGGCTCAGT CTATGACATT
    TATCTACCAA CACATATTCA GTGTAGCATC AAACCCCATG CAATCATTAT CCTCCCCAAC
    ACGGATGGGA TGGAGCTGCT GGTGTGCTAC GAAGACGAGG GGGTTTACGT TAACACGTAC
    GGAAGGATCA CCAAGGACGT GGTTCTGCAG TGGGGAGAGA TGCCGACATC TGTAGCATAT
    ATCCGGTCGA ATCAGACAAT GGGCTGGGGA GAGAAGGCAA TAGAGATACG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT ATTTATGCAC AAAAGGGCTC AGAGACTAAA GTTCCTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCC GTCCGCTCTG GTGGCAGCAG CCAGGTCTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176507.1
    CDS319..4077
    Translation

    Target ORF information:

    RefSeq Version XM_017321018.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321018.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTTCCCGTG AGAACGACCT CTCGTTCCCC TGTCCTCTCC
    CGTCGGGATT CCCCACTGCA GGGCGGTGGA CAGCAGAATA GCCAAGCAGG ACAGAGAAAT
    TCCACCAGCA GTATTGAGCC CCGGCTTCTT TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG
    CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC AACTCAGGAT CCCAGCCTGG CTCCCATCCT
    GGGTCTCAGA GCGGCTCCGG TGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGT
    TCTCCATCGC CACGCCAGGA AAGTGCAGCC AAAAAGCCTG ACGATAAGAA AGAAGTATTC
    CGGCCTCTCA AGCCTGCGGG AGAAGTGGAC TTGACTGCGT TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TTCGGCCACC TCACAAAGTA ACAGACTACT CATCCTCCAG TGAGGAGTCT
    GGGACCACAG ATGAGGAGGA GGAGGACGTG GAACAGGAGG GGGCTGATGA TTCTACCTCG
    GGACCAGAAG ACACCAGAGC AGCGTCATCT CCGAACCTGA GCAACGGTGA AACAGAATCT
    GTGAAAACAA TGATCGTTCA TGATGATGTA GAAAGTGAGC CAGCCATGAC CCCGTCCAAG
    GAGGGCACCC TCATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCGGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC TTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGGTT ACTACAGATC
    TCTCCATCTA GTGGGACGAC AGTGACTTCC GTGGTGGGAT TTTCCTGTGA TGGGCTGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACTCGG AAAGGCTCAG TGGTCAATGT GAACCCCACG
    AACACTAGGC CACAGAGTGA TACCCCCGAG ATTCGTAAAT ACAAGAAGAG ATTTAACTCA
    GAGATCCTGT GTGCTGCCTT ATGGGGAGTG AACTTGCTGG TTGGTACAGA GAGCGGTCTG
    ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA GTATACCCTC TGATCAGCCG AAGACGGTTC
    CAGCAAATGG ATGTGCTCGA AGGCCTAAAT GTCCTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT ATCCTGGCTA AGAAATAAGA TTCTTCATAA TGATCCAGAG
    GTTGAGAAGA AGCAGGGGTG GACCACTGTG GGCGACTTGG AAGGGTGCGT GCACTATAAA
    GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG GTAATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCATGGG CACCGAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAACTG
    CTACATAAGC CATTACTGGT GGATCTCACT GTGGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCGGGATT CCATGCTGTT GATGTGGATT CAGGCTCAGT CTATGACATT
    TATCTACCAA CACATATTCA GTGTAGCATC AAACCCCATG CAATCATTAT CCTCCCCAAC
    ACGGATGGGA TGGAGCTGCT GGTGTGCTAC GAAGACGAGG GGGTTTACGT TAACACGTAC
    GGAAGGATCA CCAAGGACGT GGTTCTGCAG TGGGGAGAGA TGCCGACATC TGTAGCATAT
    ATCCGGTCGA ATCAGACAAT GGGCTGGGGA GAGAAGGCAA TAGAGATACG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT ATTTATGCAC AAAAGGGCTC AGAGACTAAA GTTCCTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCC GTCCGCTCTG GTGGCAGCAG CCAGGTCTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77161
    Clone ID Related Accession (Same CDS sequence) XM_017321018.1 , XM_017321018.2
    Accession Version XM_017321018.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3759bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321018.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTTCCCGTG AGAACGACCT CTCGTTCCCC TGTCCTCTCC
    CGTCGGGATT CCCCACTGCA GGGCGGTGGA CAGCAGAATA GCCAAGCAGG ACAGAGAAAT
    TCCACCAGCA GTATTGAGCC CCGGCTTCTT TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG
    CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC AACTCAGGAT CCCAGCCTGG CTCCCATCCT
    GGGTCTCAGA GCGGCTCCGG TGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGT
    TCTCCATCGC CACGCCAGGA AAGTGCAGCC AAAAAGCCTG ACGATAAGAA AGAAGTATTC
    CGGCCTCTCA AGCCTGCGGG AGAAGTGGAC TTGACTGCGT TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TTCGGCCACC TCACAAAGTA ACAGACTACT CATCCTCCAG TGAGGAGTCT
    GGGACCACAG ATGAGGAGGA GGAGGACGTG GAACAGGAGG GGGCTGATGA TTCTACCTCG
    GGACCAGAAG ACACCAGAGC AGCGTCATCT CCGAACCTGA GCAACGGTGA AACAGAATCT
    GTGAAAACAA TGATCGTTCA TGATGATGTA GAAAGTGAGC CAGCCATGAC CCCGTCCAAG
    GAGGGCACCC TCATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCGGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC TTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGGTT ACTACAGATC
    TCTCCATCTA GTGGGACGAC AGTGACTTCC GTGGTGGGAT TTTCCTGTGA TGGGCTGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACTCGG AAAGGCTCAG TGGTCAATGT GAACCCCACG
    AACACTAGGC CACAGAGTGA TACCCCCGAG ATTCGTAAAT ACAAGAAGAG ATTTAACTCA
    GAGATCCTGT GTGCTGCCTT ATGGGGAGTG AACTTGCTGG TTGGTACAGA GAGCGGTCTG
    ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA GTATACCCTC TGATCAGCCG AAGACGGTTC
    CAGCAAATGG ATGTGCTCGA AGGCCTAAAT GTCCTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT ATCCTGGCTA AGAAATAAGA TTCTTCATAA TGATCCAGAG
    GTTGAGAAGA AGCAGGGGTG GACCACTGTG GGCGACTTGG AAGGGTGCGT GCACTATAAA
    GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG GTAATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCATGGG CACCGAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAACTG
    CTACATAAGC CATTACTGGT GGATCTCACT GTGGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCGGGATT CCATGCTGTT GATGTGGATT CAGGCTCAGT CTATGACATT
    TATCTACCAA CACATATTCA GTGTAGCATC AAACCCCATG CAATCATTAT CCTCCCCAAC
    ACGGATGGGA TGGAGCTGCT GGTGTGCTAC GAAGACGAGG GGGTTTACGT TAACACGTAC
    GGAAGGATCA CCAAGGACGT GGTTCTGCAG TGGGGAGAGA TGCCGACATC TGTAGCATAT
    ATCCGGTCGA ATCAGACAAT GGGCTGGGGA GAGAAGGCAA TAGAGATACG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT ATTTATGCAC AAAAGGGCTC AGAGACTAAA GTTCCTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCC GTCCGCTCTG GTGGCAGCAG CCAGGTCTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176507.1
    CDS329..4087
    Translation

    Target ORF information:

    RefSeq Version XM_017321018.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321018.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTTCCCGTG AGAACGACCT CTCGTTCCCC TGTCCTCTCC
    CGTCGGGATT CCCCACTGCA GGGCGGTGGA CAGCAGAATA GCCAAGCAGG ACAGAGAAAT
    TCCACCAGCA GTATTGAGCC CCGGCTTCTT TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG
    CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC AACTCAGGAT CCCAGCCTGG CTCCCATCCT
    GGGTCTCAGA GCGGCTCCGG TGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGT
    TCTCCATCGC CACGCCAGGA AAGTGCAGCC AAAAAGCCTG ACGATAAGAA AGAAGTATTC
    CGGCCTCTCA AGCCTGCGGG AGAAGTGGAC TTGACTGCGT TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TTCGGCCACC TCACAAAGTA ACAGACTACT CATCCTCCAG TGAGGAGTCT
    GGGACCACAG ATGAGGAGGA GGAGGACGTG GAACAGGAGG GGGCTGATGA TTCTACCTCG
    GGACCAGAAG ACACCAGAGC AGCGTCATCT CCGAACCTGA GCAACGGTGA AACAGAATCT
    GTGAAAACAA TGATCGTTCA TGATGATGTA GAAAGTGAGC CAGCCATGAC CCCGTCCAAG
    GAGGGCACCC TCATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCGGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC TTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGGTT ACTACAGATC
    TCTCCATCTA GTGGGACGAC AGTGACTTCC GTGGTGGGAT TTTCCTGTGA TGGGCTGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACTCGG AAAGGCTCAG TGGTCAATGT GAACCCCACG
    AACACTAGGC CACAGAGTGA TACCCCCGAG ATTCGTAAAT ACAAGAAGAG ATTTAACTCA
    GAGATCCTGT GTGCTGCCTT ATGGGGAGTG AACTTGCTGG TTGGTACAGA GAGCGGTCTG
    ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA GTATACCCTC TGATCAGCCG AAGACGGTTC
    CAGCAAATGG ATGTGCTCGA AGGCCTAAAT GTCCTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT ATCCTGGCTA AGAAATAAGA TTCTTCATAA TGATCCAGAG
    GTTGAGAAGA AGCAGGGGTG GACCACTGTG GGCGACTTGG AAGGGTGCGT GCACTATAAA
    GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG GTAATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCATGGG CACCGAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAACTG
    CTACATAAGC CATTACTGGT GGATCTCACT GTGGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCGGGATT CCATGCTGTT GATGTGGATT CAGGCTCAGT CTATGACATT
    TATCTACCAA CACATATTCA GTGTAGCATC AAACCCCATG CAATCATTAT CCTCCCCAAC
    ACGGATGGGA TGGAGCTGCT GGTGTGCTAC GAAGACGAGG GGGTTTACGT TAACACGTAC
    GGAAGGATCA CCAAGGACGT GGTTCTGCAG TGGGGAGAGA TGCCGACATC TGTAGCATAT
    ATCCGGTCGA ATCAGACAAT GGGCTGGGGA GAGAAGGCAA TAGAGATACG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT ATTTATGCAC AAAAGGGCTC AGAGACTAAA GTTCCTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCC GTCCGCTCTG GTGGCAGCAG CCAGGTCTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77162
    Clone ID Related Accession (Same CDS sequence) XM_017321021.2 , XM_017321021.1
    Accession Version XM_017321021.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017321021.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTTCCCGTG AGAACGACCT CTCGTTCCCC TGTCCTCTCC
    CGTCGGGATT CCCCACTGCA GGGCGGTGGA CAGCAGAATA GCCAAGCAGG ACAGAGAAAT
    TCCACCAGCA GTATTGAGCC CCGGCTTCTT TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG
    CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC AACTCAGGAT CCCAGCCTGG CTCCCATCCT
    GGGTCTCAGA GCGGCTCCGG TGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGT
    TCTCCATCGC CACGCCAGGA AAGTGCAGCC AAAAAGCCTG ACGATAAGAA AGAAGTATTC
    CGGCCTCTCA AGCCTGCGGA CTTGACTGCG TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTTCGGCCAC CTCACAAAGT AACAGACTAC TCATCCTCCA GTGAGGAGTC TGGGACCACA
    GATGAGGAGG AGGAGGACGT GGAACAGGAG GGGGCTGATG ATTCTACCTC GGGACCAGAA
    GACACCAGAG CAGCGTCATC TCCGAACCTG AGCAACGGTG AAACAGAATC TGTGAAAACA
    ATGATCGTTC ATGATGATGT AGAAAGTGAG CCAGCCATGA CCCCGTCCAA GGAGGGCACC
    CTCATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC
    TTTGCGGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CTTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGGT TACTACAGAT CTCTCCATCT
    AGTGGGACGA CAGTGACTTC CGTGGTGGGA TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACTCG GAAAGGCTCA GTGGTCAATG TGAACCCCAC GAACACTAGG
    CCACAGAGTG ATACCCCCGA GATTCGTAAA TACAAGAAGA GATTTAACTC AGAGATCCTG
    TGTGCTGCCT TATGGGGAGT GAACTTGCTG GTTGGTACAG AGAGCGGTCT GATGCTCTTG
    GACAGAAGTG GCCAAGGGAA AGTATACCCT CTGATCAGCC GAAGACGGTT CCAGCAAATG
    GATGTGCTCG AAGGCCTAAA TGTCCTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TATCCTGGCT AAGAAATAAG ATTCTTCATA ATGATCCAGA GGTTGAGAAG
    AAGCAGGGGT GGACCACTGT GGGCGACTTG GAAGGGTGCG TGCACTATAA AGTCGTAAAA
    TATGAAAGAA TCAAGTTTCT GGTAATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCATGG
    GCACCGAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAACT GCTACATAAG
    CCATTACTGG TGGATCTCAC TGTGGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCGGGAT TCCATGCTGT TGATGTGGAT TCAGGCTCAG TCTATGACAT TTATCTACCA
    ACACATATTC AGTGTAGCAT CAAACCCCAT GCAATCATTA TCCTCCCCAA CACGGATGGG
    ATGGAGCTGC TGGTGTGCTA CGAAGACGAG GGGGTTTACG TTAACACGTA CGGAAGGATC
    ACCAAGGACG TGGTTCTGCA GTGGGGAGAG ATGCCGACAT CTGTAGCATA TATCCGGTCG
    AATCAGACAA TGGGCTGGGG AGAGAAGGCA ATAGAGATAC GATCTGTGGA AACTGGTCAC
    TTGGATGGTG TATTTATGCA CAAAAGGGCT CAGAGACTAA AGTTCCTGTG TGAACGCAAT
    GACAAGGTGT TCTTTGCCTC CGTCCGCTCT GGTGGCAGCA GCCAGGTCTA TTTCATGACC
    TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176510.1
    CDS329..4078
    Translation

    Target ORF information:

    RefSeq Version XM_017321021.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321021.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTTCCCGTG AGAACGACCT CTCGTTCCCC TGTCCTCTCC
    CGTCGGGATT CCCCACTGCA GGGCGGTGGA CAGCAGAATA GCCAAGCAGG ACAGAGAAAT
    TCCACCAGCA GTATTGAGCC CCGGCTTCTT TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG
    CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC AACTCAGGAT CCCAGCCTGG CTCCCATCCT
    GGGTCTCAGA GCGGCTCCGG TGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGT
    TCTCCATCGC CACGCCAGGA AAGTGCAGCC AAAAAGCCTG ACGATAAGAA AGAAGTATTC
    CGGCCTCTCA AGCCTGCGGA CTTGACTGCG TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTTCGGCCAC CTCACAAAGT AACAGACTAC TCATCCTCCA GTGAGGAGTC TGGGACCACA
    GATGAGGAGG AGGAGGACGT GGAACAGGAG GGGGCTGATG ATTCTACCTC GGGACCAGAA
    GACACCAGAG CAGCGTCATC TCCGAACCTG AGCAACGGTG AAACAGAATC TGTGAAAACA
    ATGATCGTTC ATGATGATGT AGAAAGTGAG CCAGCCATGA CCCCGTCCAA GGAGGGCACC
    CTCATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC
    TTTGCGGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CTTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGGT TACTACAGAT CTCTCCATCT
    AGTGGGACGA CAGTGACTTC CGTGGTGGGA TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACTCG GAAAGGCTCA GTGGTCAATG TGAACCCCAC GAACACTAGG
    CCACAGAGTG ATACCCCCGA GATTCGTAAA TACAAGAAGA GATTTAACTC AGAGATCCTG
    TGTGCTGCCT TATGGGGAGT GAACTTGCTG GTTGGTACAG AGAGCGGTCT GATGCTCTTG
    GACAGAAGTG GCCAAGGGAA AGTATACCCT CTGATCAGCC GAAGACGGTT CCAGCAAATG
    GATGTGCTCG AAGGCCTAAA TGTCCTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TATCCTGGCT AAGAAATAAG ATTCTTCATA ATGATCCAGA GGTTGAGAAG
    AAGCAGGGGT GGACCACTGT GGGCGACTTG GAAGGGTGCG TGCACTATAA AGTCGTAAAA
    TATGAAAGAA TCAAGTTTCT GGTAATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCATGG
    GCACCGAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAACT GCTACATAAG
    CCATTACTGG TGGATCTCAC TGTGGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCGGGAT TCCATGCTGT TGATGTGGAT TCAGGCTCAG TCTATGACAT TTATCTACCA
    ACACATATTC AGTGTAGCAT CAAACCCCAT GCAATCATTA TCCTCCCCAA CACGGATGGG
    ATGGAGCTGC TGGTGTGCTA CGAAGACGAG GGGGTTTACG TTAACACGTA CGGAAGGATC
    ACCAAGGACG TGGTTCTGCA GTGGGGAGAG ATGCCGACAT CTGTAGCATA TATCCGGTCG
    AATCAGACAA TGGGCTGGGG AGAGAAGGCA ATAGAGATAC GATCTGTGGA AACTGGTCAC
    TTGGATGGTG TATTTATGCA CAAAAGGGCT CAGAGACTAA AGTTCCTGTG TGAACGCAAT
    GACAAGGTGT TCTTTGCCTC CGTCCGCTCT GGTGGCAGCA GCCAGGTCTA TTTCATGACC
    TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu77162
    Clone ID Related Accession (Same CDS sequence) XM_017321021.2 , XM_017321021.1
    Accession Version XM_017321021.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTTCCCGTG AGAACGACCT CTCGTTCCCC TGTCCTCTCC
    CGTCGGGATT CCCCACTGCA GGGCGGTGGA CAGCAGAATA GCCAAGCAGG ACAGAGAAAT
    TCCACCAGCA GTATTGAGCC CCGGCTTCTT TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG
    CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC AACTCAGGAT CCCAGCCTGG CTCCCATCCT
    GGGTCTCAGA GCGGCTCCGG TGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGT
    TCTCCATCGC CACGCCAGGA AAGTGCAGCC AAAAAGCCTG ACGATAAGAA AGAAGTATTC
    CGGCCTCTCA AGCCTGCGGA CTTGACTGCG TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTTCGGCCAC CTCACAAAGT AACAGACTAC TCATCCTCCA GTGAGGAGTC TGGGACCACA
    GATGAGGAGG AGGAGGACGT GGAACAGGAG GGGGCTGATG ATTCTACCTC GGGACCAGAA
    GACACCAGAG CAGCGTCATC TCCGAACCTG AGCAACGGTG AAACAGAATC TGTGAAAACA
    ATGATCGTTC ATGATGATGT AGAAAGTGAG CCAGCCATGA CCCCGTCCAA GGAGGGCACC
    CTCATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC
    TTTGCGGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CTTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGGT TACTACAGAT CTCTCCATCT
    AGTGGGACGA CAGTGACTTC CGTGGTGGGA TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACTCG GAAAGGCTCA GTGGTCAATG TGAACCCCAC GAACACTAGG
    CCACAGAGTG ATACCCCCGA GATTCGTAAA TACAAGAAGA GATTTAACTC AGAGATCCTG
    TGTGCTGCCT TATGGGGAGT GAACTTGCTG GTTGGTACAG AGAGCGGTCT GATGCTCTTG
    GACAGAAGTG GCCAAGGGAA AGTATACCCT CTGATCAGCC GAAGACGGTT CCAGCAAATG
    GATGTGCTCG AAGGCCTAAA TGTCCTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TATCCTGGCT AAGAAATAAG ATTCTTCATA ATGATCCAGA GGTTGAGAAG
    AAGCAGGGGT GGACCACTGT GGGCGACTTG GAAGGGTGCG TGCACTATAA AGTCGTAAAA
    TATGAAAGAA TCAAGTTTCT GGTAATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCATGG
    GCACCGAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAACT GCTACATAAG
    CCATTACTGG TGGATCTCAC TGTGGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCGGGAT TCCATGCTGT TGATGTGGAT TCAGGCTCAG TCTATGACAT TTATCTACCA
    ACACATATTC AGTGTAGCAT CAAACCCCAT GCAATCATTA TCCTCCCCAA CACGGATGGG
    ATGGAGCTGC TGGTGTGCTA CGAAGACGAG GGGGTTTACG TTAACACGTA CGGAAGGATC
    ACCAAGGACG TGGTTCTGCA GTGGGGAGAG ATGCCGACAT CTGTAGCATA TATCCGGTCG
    AATCAGACAA TGGGCTGGGG AGAGAAGGCA ATAGAGATAC GATCTGTGGA AACTGGTCAC
    TTGGATGGTG TATTTATGCA CAAAAGGGCT CAGAGACTAA AGTTCCTGTG TGAACGCAAT
    GACAAGGTGT TCTTTGCCTC CGTCCGCTCT GGTGGCAGCA GCCAGGTCTA TTTCATGACC
    TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017176510.1
    CDS319..4068
    Translation

    Target ORF information:

    RefSeq Version XM_017321021.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017321021.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTTCCCGTG AGAACGACCT CTCGTTCCCC TGTCCTCTCC
    CGTCGGGATT CCCCACTGCA GGGCGGTGGA CAGCAGAATA GCCAAGCAGG ACAGAGAAAT
    TCCACCAGCA GTATTGAGCC CCGGCTTCTT TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG
    CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC AACTCAGGAT CCCAGCCTGG CTCCCATCCT
    GGGTCTCAGA GCGGCTCCGG TGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGT
    TCTCCATCGC CACGCCAGGA AAGTGCAGCC AAAAAGCCTG ACGATAAGAA AGAAGTATTC
    CGGCCTCTCA AGCCTGCGGA CTTGACTGCG TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTTCGGCCAC CTCACAAAGT AACAGACTAC TCATCCTCCA GTGAGGAGTC TGGGACCACA
    GATGAGGAGG AGGAGGACGT GGAACAGGAG GGGGCTGATG ATTCTACCTC GGGACCAGAA
    GACACCAGAG CAGCGTCATC TCCGAACCTG AGCAACGGTG AAACAGAATC TGTGAAAACA
    ATGATCGTTC ATGATGATGT AGAAAGTGAG CCAGCCATGA CCCCGTCCAA GGAGGGCACC
    CTCATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC
    TTTGCGGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CTTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGGT TACTACAGAT CTCTCCATCT
    AGTGGGACGA CAGTGACTTC CGTGGTGGGA TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACTCG GAAAGGCTCA GTGGTCAATG TGAACCCCAC GAACACTAGG
    CCACAGAGTG ATACCCCCGA GATTCGTAAA TACAAGAAGA GATTTAACTC AGAGATCCTG
    TGTGCTGCCT TATGGGGAGT GAACTTGCTG GTTGGTACAG AGAGCGGTCT GATGCTCTTG
    GACAGAAGTG GCCAAGGGAA AGTATACCCT CTGATCAGCC GAAGACGGTT CCAGCAAATG
    GATGTGCTCG AAGGCCTAAA TGTCCTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TATCCTGGCT AAGAAATAAG ATTCTTCATA ATGATCCAGA GGTTGAGAAG
    AAGCAGGGGT GGACCACTGT GGGCGACTTG GAAGGGTGCG TGCACTATAA AGTCGTAAAA
    TATGAAAGAA TCAAGTTTCT GGTAATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCATGG
    GCACCGAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAACT GCTACATAAG
    CCATTACTGG TGGATCTCAC TGTGGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCGGGAT TCCATGCTGT TGATGTGGAT TCAGGCTCAG TCTATGACAT TTATCTACCA
    ACACATATTC AGTGTAGCAT CAAACCCCAT GCAATCATTA TCCTCCCCAA CACGGATGGG
    ATGGAGCTGC TGGTGTGCTA CGAAGACGAG GGGGTTTACG TTAACACGTA CGGAAGGATC
    ACCAAGGACG TGGTTCTGCA GTGGGGAGAG ATGCCGACAT CTGTAGCATA TATCCGGTCG
    AATCAGACAA TGGGCTGGGG AGAGAAGGCA ATAGAGATAC GATCTGTGGA AACTGGTCAC
    TTGGATGGTG TATTTATGCA CAAAAGGGCT CAGAGACTAA AGTTCCTGTG TGAACGCAAT
    GACAAGGTGT TCTTTGCCTC CGTCCGCTCT GGTGGCAGCA GCCAGGTCTA TTTCATGACC
    TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85180
    Clone ID Related Accession (Same CDS sequence) XM_030254710.1
    Accession Version XM_030254710.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTACAGTGG TCCCACCTGG CATCTCTCAA GAACAATGTC
    TCCCCTGTCT CGAGATCCCA TTCCTTCAGT GACCCTTCTC CCAAATTCGC ACACCACCAT
    CTCCGCTCTC AGGACCCATG TCCACCTTCC CGCAGTGAGG GGCTCAGTCA GAGCTCTGAC
    TCTAAGTCGG AGGTGCCCGA GCCCACCCAA AAGGCTTGGT CTAGATCAGA CAGTGACGAG
    GTGCCTCCAA GGGTTCCCGT GAGAACGACC TCTCGTTCCC CTGTCCTCTC CCGTCGGGAT
    TCCCCACTGC AGGGCGGTGG ACAGCAGAAT AGCCAAGCAG GACAGAGAAA TTCCACCAGT
    ATTGAGCCCC GGCTTCTTTG GGAGAGAGTG GAAAAGCTGG TCCCCAGGCC AGGCAGTGGC
    AGCTCTTCAG GGTCCAGCAA CTCAGGATCC CAGCCTGGCT CCCATCCTGG GTCTCAGAGC
    GGCTCCGGTG AACGCTTCAG AGTGAGATCA TCATCCAAGT CTGAAGGTTC TCCATCGCCA
    CGCCAGGAAA GTGCAGCCAA AAAGCCTGAC GATAAGAAAG AAGTATTCCG GCCTCTCAAG
    CCTGCGGGAG AAGTGGACTT GACTGCGTTG GCCAAAGAGC TTCGAGCAGT GGAAGATGTT
    CGGCCACCTC ACAAAGTAAC AGACTACTCA TCCTCCAGTG AGGAGTCTGG GACCACAGAT
    GAGGAGGAGG AGGACGTGGA ACAGGAGGGG GCTGATGATT CTACCTCGGG ACCAGAAGAC
    ACCAGAGCAG CGTCATCTCC GAACCTGAGC AACGGTGAAA CAGAATCTGT GAAAACAATG
    ATCGTTCATG ATGATGTAGA AAGTGAGCCA GCCATGACCC CGTCCAAGGA GGGCACCCTC
    ATCGTCCGCC AGAGTACAGT TGACCAAAAG CGTGCCAGCC ATCATGAGAG CAATGGCTTT
    GCGGGTCGCA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACTTCC
    TCCACCTCTT CCTCCCCATC CTCCAGCCAG CCGACACCCA CCATGTCCCC ACAGACACCC
    CAGGACAAGC TCACTGCTAA TGAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGGTTAC TACAGATCTC TCCATCTAGT
    GGGACGACAG TGACTTCCGT GGTGGGATTT TCCTGTGATG GGCTGAGACC AGAAGCCATA
    AGGCAAGATC CTACTCGGAA AGGCTCAGTG GTCAATGTGA ACCCCACGAA CACTAGGCCA
    CAGAGTGATA CCCCCGAGAT TCGTAAATAC AAGAAGAGAT TTAACTCAGA GATCCTGTGT
    GCTGCCTTAT GGGGAGTGAA CTTGCTGGTT GGTACAGAGA GCGGTCTGAT GCTCTTGGAC
    AGAAGTGGCC AAGGGAAAGT ATACCCTCTG ATCAGCCGAA GACGGTTCCA GCAAATGGAT
    GTGCTCGAAG GCCTAAATGT CCTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC
    TACTATTTAT CCTGGCTAAG AAATAAGATT CTTCATAATG ATCCAGAGGT TGAGAAGAAG
    CAGGGGTGGA CCACTGTGGG CGACTTGGAA GGGTGCGTGC ACTATAAAGT CGTAAAATAT
    GAAAGAATCA AGTTTCTGGT AATTGCTTTG AAGAGTTCTG TGGAAGTCTA TGCATGGGCA
    CCGAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAACTGCT ACATAAGCCA
    TTACTGGTGG ATCTCACTGT GGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT
    GCGGGATTCC ATGCTGTTGA TGTGGATTCA GGCTCAGTCT ATGACATTTA TCTACCAACA
    CATATTCAGT GTAGCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGGATG
    GAGCTGCTGG TGTGCTACGA AGACGAGGGG GTTTACGTTA ACACGTACGG AAGGATCACC
    AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCGACATCTG TAGCATATAT CCGGTCGAAT
    CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGAT CTGTGGAAAC TGGTCACTTG
    GATGGTGTAT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGCAATGAC
    AAGGTGTTCT TTGCCTCCGT CCGCTCTGGT GGCAGCAGCC AGGTCTATTT CATGACCTTA
    GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110570.1
    CDS329..4315
    Translation

    Target ORF information:

    RefSeq Version XM_030254710.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254710.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTACAGTGG TCCCACCTGG CATCTCTCAA GAACAATGTC
    TCCCCTGTCT CGAGATCCCA TTCCTTCAGT GACCCTTCTC CCAAATTCGC ACACCACCAT
    CTCCGCTCTC AGGACCCATG TCCACCTTCC CGCAGTGAGG GGCTCAGTCA GAGCTCTGAC
    TCTAAGTCGG AGGTGCCCGA GCCCACCCAA AAGGCTTGGT CTAGATCAGA CAGTGACGAG
    GTGCCTCCAA GGGTTCCCGT GAGAACGACC TCTCGTTCCC CTGTCCTCTC CCGTCGGGAT
    TCCCCACTGC AGGGCGGTGG ACAGCAGAAT AGCCAAGCAG GACAGAGAAA TTCCACCAGT
    ATTGAGCCCC GGCTTCTTTG GGAGAGAGTG GAAAAGCTGG TCCCCAGGCC AGGCAGTGGC
    AGCTCTTCAG GGTCCAGCAA CTCAGGATCC CAGCCTGGCT CCCATCCTGG GTCTCAGAGC
    GGCTCCGGTG AACGCTTCAG AGTGAGATCA TCATCCAAGT CTGAAGGTTC TCCATCGCCA
    CGCCAGGAAA GTGCAGCCAA AAAGCCTGAC GATAAGAAAG AAGTATTCCG GCCTCTCAAG
    CCTGCGGGAG AAGTGGACTT GACTGCGTTG GCCAAAGAGC TTCGAGCAGT GGAAGATGTT
    CGGCCACCTC ACAAAGTAAC AGACTACTCA TCCTCCAGTG AGGAGTCTGG GACCACAGAT
    GAGGAGGAGG AGGACGTGGA ACAGGAGGGG GCTGATGATT CTACCTCGGG ACCAGAAGAC
    ACCAGAGCAG CGTCATCTCC GAACCTGAGC AACGGTGAAA CAGAATCTGT GAAAACAATG
    ATCGTTCATG ATGATGTAGA AAGTGAGCCA GCCATGACCC CGTCCAAGGA GGGCACCCTC
    ATCGTCCGCC AGAGTACAGT TGACCAAAAG CGTGCCAGCC ATCATGAGAG CAATGGCTTT
    GCGGGTCGCA TTCACCTCTT GCCAGATCTC TTACAGCAAA GCCATTCCTC CTCCACTTCC
    TCCACCTCTT CCTCCCCATC CTCCAGCCAG CCGACACCCA CCATGTCCCC ACAGACACCC
    CAGGACAAGC TCACTGCTAA TGAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGGTTAC TACAGATCTC TCCATCTAGT
    GGGACGACAG TGACTTCCGT GGTGGGATTT TCCTGTGATG GGCTGAGACC AGAAGCCATA
    AGGCAAGATC CTACTCGGAA AGGCTCAGTG GTCAATGTGA ACCCCACGAA CACTAGGCCA
    CAGAGTGATA CCCCCGAGAT TCGTAAATAC AAGAAGAGAT TTAACTCAGA GATCCTGTGT
    GCTGCCTTAT GGGGAGTGAA CTTGCTGGTT GGTACAGAGA GCGGTCTGAT GCTCTTGGAC
    AGAAGTGGCC AAGGGAAAGT ATACCCTCTG ATCAGCCGAA GACGGTTCCA GCAAATGGAT
    GTGCTCGAAG GCCTAAATGT CCTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC
    TACTATTTAT CCTGGCTAAG AAATAAGATT CTTCATAATG ATCCAGAGGT TGAGAAGAAG
    CAGGGGTGGA CCACTGTGGG CGACTTGGAA GGGTGCGTGC ACTATAAAGT CGTAAAATAT
    GAAAGAATCA AGTTTCTGGT AATTGCTTTG AAGAGTTCTG TGGAAGTCTA TGCATGGGCA
    CCGAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAACTGCT ACATAAGCCA
    TTACTGGTGG ATCTCACTGT GGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT
    GCGGGATTCC ATGCTGTTGA TGTGGATTCA GGCTCAGTCT ATGACATTTA TCTACCAACA
    CATATTCAGT GTAGCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGGATG
    GAGCTGCTGG TGTGCTACGA AGACGAGGGG GTTTACGTTA ACACGTACGG AAGGATCACC
    AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCGACATCTG TAGCATATAT CCGGTCGAAT
    CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGAT CTGTGGAAAC TGGTCACTTG
    GATGGTGTAT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGCAATGAC
    AAGGTGTTCT TTGCCTCCGT CCGCTCTGGT GGCAGCAGCC AGGTCTATTT CATGACCTTA
    GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85181
    Clone ID Related Accession (Same CDS sequence) XM_030254720.1
    Accession Version XM_030254720.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT ACAGTGGTCC
    CACCTGGCAT CTCTCAAGAA CAATGTCTCC CCTGTCTCGA GATCCCATTC CTTCAGTGAC
    CCTTCTCCCA AATTCGCACA CCACCATCTC CGCTCTCAGG ACCCATGTCC ACCTTCCCGC
    AGTGAGGGGC TCAGTCAGAG CTCTGACTCT AAGTCGGAGG TGCCCGAGCC CACCCAAAAG
    GCTTGGTCTA GATCAGACAG TGACGAGGTG CCTCCAAGGG TTCCCGTGAG AACGACCTCT
    CGTTCCCCTG TCCTCTCCCG TCGGGATTCC CCACTGCAGG GCGGTGGACA GCAGAATAGC
    CAAGCAGGAC AGAGAAATTC CACCAGCAGT ATTGAGCCCC GGCTTCTTTG GGAGAGAGTG
    GAAAAGCTGG TCCCCAGGCC AGGCAGTGGC AGCTCTTCAG GGTCCAGCAA CTCAGGATCC
    CAGCCTGGCT CCCATCCTGG GTCTCAGAGC GGCTCCGGTG AACGCTTCAG AGTGAGATCA
    TCATCCAAGT CTGAAGGTTC TCCATCGCCA CGCCAGGAAA GTGCAGCCAA AAAGCCTGAC
    GATAAGAAAG AAGTATTCCG GCCTCTCAAG CCTGCGGACT TGACTGCGTT GGCCAAAGAG
    CTTCGAGCAG TGGAAGATGT TCGGCCACCT CACAAAGTAA CAGACTACTC ATCCTCCAGT
    GAGGAGTCTG GGACCACAGA TGAGGAGGAG GAGGACGTGG AACAGGAGGG GGCTGATGAT
    TCTACCTCGG GACCAGAAGA CACCAGAGCA GCGTCATCTC CGAACCTGAG CAACGGTGAA
    ACAGAATCTG TGAAAACAAT GATCGTTCAT GATGATGTAG AAAGTGAGCC AGCCATGACC
    CCGTCCAAGG AGGGCACCCT CATCGTCCGC CAGAGTACAG TTGACCAAAA GCGTGCCAGC
    CATCATGAGA GCAATGGCTT TGCGGGTCGC ATTCACCTCT TGCCAGATCT CTTACAGCAA
    AGCCATTCCT CCTCCACTTC CTCCACCTCT TCCTCCCCAT CCTCCAGCCA GCCGACACCC
    ACCATGTCCC CACAGACACC CCAGGACAAG CTCACTGCTA ATGAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGGTTA
    CTACAGATCT CTCCATCTAG TGGGACGACA GTGACTTCCG TGGTGGGATT TTCCTGTGAT
    GGGCTGAGAC CAGAAGCCAT AAGGCAAGAT CCTACTCGGA AAGGCTCAGT GGTCAATGTG
    AACCCCACGA ACACTAGGCC ACAGAGTGAT ACCCCCGAGA TTCGTAAATA CAAGAAGAGA
    TTTAACTCAG AGATCCTGTG TGCTGCCTTA TGGGGAGTGA ACTTGCTGGT TGGTACAGAG
    AGCGGTCTGA TGCTCTTGGA CAGAAGTGGC CAAGGGAAAG TATACCCTCT GATCAGCCGA
    AGACGGTTCC AGCAAATGGA TGTGCTCGAA GGCCTAAATG TCCTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTA TCCTGGCTAA GAAATAAGAT TCTTCATAAT
    GATCCAGAGG TTGAGAAGAA GCAGGGGTGG ACCACTGTGG GCGACTTGGA AGGGTGCGTG
    CACTATAAAG TCGTAAAATA TGAAAGAATC AAGTTTCTGG TAATTGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCATGGGC ACCGAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAACTGC TACATAAGCC ATTACTGGTG GATCTCACTG TGGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCGGGATTC CATGCTGTTG ATGTGGATTC AGGCTCAGTC
    TATGACATTT ATCTACCAAC ACATATTCAG TGTAGCATCA AACCCCATGC AATCATTATC
    CTCCCCAACA CGGATGGGAT GGAGCTGCTG GTGTGCTACG AAGACGAGGG GGTTTACGTT
    AACACGTACG GAAGGATCAC CAAGGACGTG GTTCTGCAGT GGGGAGAGAT GCCGACATCT
    GTAGCATATA TCCGGTCGAA TCAGACAATG GGCTGGGGAG AGAAGGCAAT AGAGATACGA
    TCTGTGGAAA CTGGTCACTT GGATGGTGTA TTTATGCACA AAAGGGCTCA GAGACTAAAG
    TTCCTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCCG TCCGCTCTGG TGGCAGCAGC
    CAGGTCTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030110580.1
    CDS329..4216
    Translation

    Target ORF information:

    RefSeq Version XM_030254720.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030254720.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT ACAGTGGTCC
    CACCTGGCAT CTCTCAAGAA CAATGTCTCC CCTGTCTCGA GATCCCATTC CTTCAGTGAC
    CCTTCTCCCA AATTCGCACA CCACCATCTC CGCTCTCAGG ACCCATGTCC ACCTTCCCGC
    AGTGAGGGGC TCAGTCAGAG CTCTGACTCT AAGTCGGAGG TGCCCGAGCC CACCCAAAAG
    GCTTGGTCTA GATCAGACAG TGACGAGGTG CCTCCAAGGG TTCCCGTGAG AACGACCTCT
    CGTTCCCCTG TCCTCTCCCG TCGGGATTCC CCACTGCAGG GCGGTGGACA GCAGAATAGC
    CAAGCAGGAC AGAGAAATTC CACCAGCAGT ATTGAGCCCC GGCTTCTTTG GGAGAGAGTG
    GAAAAGCTGG TCCCCAGGCC AGGCAGTGGC AGCTCTTCAG GGTCCAGCAA CTCAGGATCC
    CAGCCTGGCT CCCATCCTGG GTCTCAGAGC GGCTCCGGTG AACGCTTCAG AGTGAGATCA
    TCATCCAAGT CTGAAGGTTC TCCATCGCCA CGCCAGGAAA GTGCAGCCAA AAAGCCTGAC
    GATAAGAAAG AAGTATTCCG GCCTCTCAAG CCTGCGGACT TGACTGCGTT GGCCAAAGAG
    CTTCGAGCAG TGGAAGATGT TCGGCCACCT CACAAAGTAA CAGACTACTC ATCCTCCAGT
    GAGGAGTCTG GGACCACAGA TGAGGAGGAG GAGGACGTGG AACAGGAGGG GGCTGATGAT
    TCTACCTCGG GACCAGAAGA CACCAGAGCA GCGTCATCTC CGAACCTGAG CAACGGTGAA
    ACAGAATCTG TGAAAACAAT GATCGTTCAT GATGATGTAG AAAGTGAGCC AGCCATGACC
    CCGTCCAAGG AGGGCACCCT CATCGTCCGC CAGAGTACAG TTGACCAAAA GCGTGCCAGC
    CATCATGAGA GCAATGGCTT TGCGGGTCGC ATTCACCTCT TGCCAGATCT CTTACAGCAA
    AGCCATTCCT CCTCCACTTC CTCCACCTCT TCCTCCCCAT CCTCCAGCCA GCCGACACCC
    ACCATGTCCC CACAGACACC CCAGGACAAG CTCACTGCTA ATGAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGGTTA
    CTACAGATCT CTCCATCTAG TGGGACGACA GTGACTTCCG TGGTGGGATT TTCCTGTGAT
    GGGCTGAGAC CAGAAGCCAT AAGGCAAGAT CCTACTCGGA AAGGCTCAGT GGTCAATGTG
    AACCCCACGA ACACTAGGCC ACAGAGTGAT ACCCCCGAGA TTCGTAAATA CAAGAAGAGA
    TTTAACTCAG AGATCCTGTG TGCTGCCTTA TGGGGAGTGA ACTTGCTGGT TGGTACAGAG
    AGCGGTCTGA TGCTCTTGGA CAGAAGTGGC CAAGGGAAAG TATACCCTCT GATCAGCCGA
    AGACGGTTCC AGCAAATGGA TGTGCTCGAA GGCCTAAATG TCCTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTA TCCTGGCTAA GAAATAAGAT TCTTCATAAT
    GATCCAGAGG TTGAGAAGAA GCAGGGGTGG ACCACTGTGG GCGACTTGGA AGGGTGCGTG
    CACTATAAAG TCGTAAAATA TGAAAGAATC AAGTTTCTGG TAATTGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCATGGGC ACCGAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAACTGC TACATAAGCC ATTACTGGTG GATCTCACTG TGGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCGGGATTC CATGCTGTTG ATGTGGATTC AGGCTCAGTC
    TATGACATTT ATCTACCAAC ACATATTCAG TGTAGCATCA AACCCCATGC AATCATTATC
    CTCCCCAACA CGGATGGGAT GGAGCTGCTG GTGTGCTACG AAGACGAGGG GGTTTACGTT
    AACACGTACG GAAGGATCAC CAAGGACGTG GTTCTGCAGT GGGGAGAGAT GCCGACATCT
    GTAGCATATA TCCGGTCGAA TCAGACAATG GGCTGGGGAG AGAAGGCAAT AGAGATACGA
    TCTGTGGAAA CTGGTCACTT GGATGGTGTA TTTATGCACA AAAGGGCTCA GAGACTAAAG
    TTCCTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCCG TCCGCTCTGG TGGCAGCAGC
    CAGGTCTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu15846
    Clone ID Related Accession (Same CDS sequence) NM_001252200.1
    Accession Version NM_001252200.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ142598.1, BB651050.1, BC145390.1, U88984.1, AV445090.1, BQ571658.1, AK159799.1 and BF721981.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145390.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTACAGTGGT CCCACCTGGC ATCTCTCAAG
    AACAATGTCT CCCCTGTCTC GAGATCCCAT TCCTTCAGTG ACCCTTCTCC CAAATTCGCA
    CACCACCATC TCCGCTCTCA GGACCCATGT CCACCTTCCC GCAGTGAGGG GCTCAGTCAG
    AGCTCTGACT CTAAGTCGGA GGTGCCCGAG CCCACCCAAA AGGCTTGGTC TAGATCAGAC
    AGTGACGAGG TGCCTCCAAG GGTTCCCGTG AGAACGACCT CTCGTTCCCC TGTCCTCTCC
    CGTCGGGATT CCCCACTGCA GGGCGGTGGA CAGCAGAATA GCCAAGCAGG ACAGAGAAAT
    TCCACCAGCA GTATTGAGCC CCGGCTTCTT TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG
    CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC AACTCAGGAT CCCAGCCTGG CTCCCATCCT
    GGGTCTCAGA GCGGCTCCGG TGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGT
    TCTCCATCGC CACGCCAGGA AAGTGCAGCC AAAAAGCCTG ACGATAAGAA AGAAGTATTC
    CGGCCTCTCA AGCCTGCGGG AGAAGTGGAC TTGACTGCGT TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TTCGGCCACC TCACAAAGTA ACAGACTACT CATCCTCCAG TGAGGAGTCT
    GGGACCACAG ATGAGGAGGA GGAGGACGTG GAACAGGAGG GGGCTGATGA TTCTACCTCG
    GGACCAGAAG ACACCAGAGC AGCGTCATCT CCGAACCTGA GCAACGGTGA AACAGAATCT
    GTGAAAACAA TGATCGTTCA TGATGATGTA GAAAGTGAGC CAGCCATGAC CCCGTCCAAG
    GAGGGCACCC TCATCGTCCG CCAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGGTTAC TACAGATCTC TCCATCTAGT
    GGGACGACAG TGACTTCCGT GGTGGGATTT TCCTGTGATG GGCTGAGACC AGAAGCCATA
    AGGCAAGATC CTACTCGGAA AGGCTCAGTG GTCAATGTGA ACCCCACGAA CACTAGGCCA
    CAGAGTGATA CCCCCGAGAT TCGTAAATAC AAGAAGAGAT TTAACTCAGA GATCCTGTGT
    GCTGCCTTAT GGGGAGTGAA CTTGCTGGTT GGTACAGAGA GCGGTCTGAT GCTCTTGGAC
    AGAAGTGGCC AAGGGAAAGT ATACCCTCTG ATCAGCCGAA GACGGTTCCA GCAAATGGAT
    GTGCTCGAAG GCCTAAATGT CCTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC
    TACTATTTAT CCTGGCTAAG AAATAAGATT CTTCATAATG ATCCAGAGGT TGAGAAGAAG
    CAGGGGTGGA CCACTGTGGG CGACTTGGAA GGGTGCGTGC ACTATAAAGT CGTAAAATAT
    GAAAGAATCA AGTTTCTGGT AATTGCTTTG AAGAGTTCTG TGGAAGTCTA TGCATGGGCA
    CCGAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAACTGCT ACATAAGCCA
    TTACTGGTGG ATCTCACTGT GGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT
    GCGGGATTCC ATGCTGTTGA TGTGGATTCA GGCTCAGTCT ATGACATTTA TCTACCAACA
    CATATTCAGT GTAGCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGGATG
    GAGCTGCTGG TGTGCTACGA AGACGAGGGG GTTTACGTTA ACACGTACGG AAGGATCACC
    AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCGACATCTG TAGCATATAT CCGGTCGAAT
    CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGAT CTGTGGAAAC TGGTCACTTG
    GATGGTGTAT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGCAATGAC
    AAGGTGTTCT TTGCCTCCGT CCGCTCTGGT GGCAGCAGCC AGGTCTATTT CATGACCTTA
    GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001239129.1
    CDS318..4184
    Translation

    Target ORF information:

    RefSeq Version NM_001252200.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001252200.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTACAGTGGT CCCACCTGGC ATCTCTCAAG
    AACAATGTCT CCCCTGTCTC GAGATCCCAT TCCTTCAGTG ACCCTTCTCC CAAATTCGCA
    CACCACCATC TCCGCTCTCA GGACCCATGT CCACCTTCCC GCAGTGAGGG GCTCAGTCAG
    AGCTCTGACT CTAAGTCGGA GGTGCCCGAG CCCACCCAAA AGGCTTGGTC TAGATCAGAC
    AGTGACGAGG TGCCTCCAAG GGTTCCCGTG AGAACGACCT CTCGTTCCCC TGTCCTCTCC
    CGTCGGGATT CCCCACTGCA GGGCGGTGGA CAGCAGAATA GCCAAGCAGG ACAGAGAAAT
    TCCACCAGCA GTATTGAGCC CCGGCTTCTT TGGGAGAGAG TGGAAAAGCT GGTCCCCAGG
    CCAGGCAGTG GCAGCTCTTC AGGGTCCAGC AACTCAGGAT CCCAGCCTGG CTCCCATCCT
    GGGTCTCAGA GCGGCTCCGG TGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGT
    TCTCCATCGC CACGCCAGGA AAGTGCAGCC AAAAAGCCTG ACGATAAGAA AGAAGTATTC
    CGGCCTCTCA AGCCTGCGGG AGAAGTGGAC TTGACTGCGT TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TTCGGCCACC TCACAAAGTA ACAGACTACT CATCCTCCAG TGAGGAGTCT
    GGGACCACAG ATGAGGAGGA GGAGGACGTG GAACAGGAGG GGGCTGATGA TTCTACCTCG
    GGACCAGAAG ACACCAGAGC AGCGTCATCT CCGAACCTGA GCAACGGTGA AACAGAATCT
    GTGAAAACAA TGATCGTTCA TGATGATGTA GAAAGTGAGC CAGCCATGAC CCCGTCCAAG
    GAGGGCACCC TCATCGTCCG CCAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGGTTAC TACAGATCTC TCCATCTAGT
    GGGACGACAG TGACTTCCGT GGTGGGATTT TCCTGTGATG GGCTGAGACC AGAAGCCATA
    AGGCAAGATC CTACTCGGAA AGGCTCAGTG GTCAATGTGA ACCCCACGAA CACTAGGCCA
    CAGAGTGATA CCCCCGAGAT TCGTAAATAC AAGAAGAGAT TTAACTCAGA GATCCTGTGT
    GCTGCCTTAT GGGGAGTGAA CTTGCTGGTT GGTACAGAGA GCGGTCTGAT GCTCTTGGAC
    AGAAGTGGCC AAGGGAAAGT ATACCCTCTG ATCAGCCGAA GACGGTTCCA GCAAATGGAT
    GTGCTCGAAG GCCTAAATGT CCTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC
    TACTATTTAT CCTGGCTAAG AAATAAGATT CTTCATAATG ATCCAGAGGT TGAGAAGAAG
    CAGGGGTGGA CCACTGTGGG CGACTTGGAA GGGTGCGTGC ACTATAAAGT CGTAAAATAT
    GAAAGAATCA AGTTTCTGGT AATTGCTTTG AAGAGTTCTG TGGAAGTCTA TGCATGGGCA
    CCGAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAACTGCT ACATAAGCCA
    TTACTGGTGG ATCTCACTGT GGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT
    GCGGGATTCC ATGCTGTTGA TGTGGATTCA GGCTCAGTCT ATGACATTTA TCTACCAACA
    CATATTCAGT GTAGCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGGATG
    GAGCTGCTGG TGTGCTACGA AGACGAGGGG GTTTACGTTA ACACGTACGG AAGGATCACC
    AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCGACATCTG TAGCATATAT CCGGTCGAAT
    CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGAT CTGTGGAAAC TGGTCACTTG
    GATGGTGTAT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGCAATGAC
    AAGGTGTTCT TTGCCTCCGT CCGCTCTGGT GGCAGCAGCC AGGTCTATTT CATGACCTTA
    GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu15949
    Clone ID Related Accession (Same CDS sequence) NM_008696.2
    Accession Version NM_008696.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-19
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC166238.7, U88984.1, CF531764.1, AC132909.14 and AK138966.1. On Apr 14, 2007 this sequence version replaced NM_008696.1. Transcript Variant: This variant (2) lacks multiple in-frame exons in the central coding region, compared to variant 1, and encodes a shorter isoform (2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U88984.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT ACAGTGGTCC
    CACCTGGCAT CTCTCAAGAA CAATGTCTCC CCTGTCTCGA GATCCCATTC CTTCAGTGAC
    CCTTCTCCCA AATTCGCACA CCACCATCTC CGCTCTCAGG ACCCATGTCC ACCTTCCCGC
    AGTGAGGGGC TCAGTCAGAG CTCTGACTCT AAGTCGGAGG TGCCCGAGCC CACCCAAAAG
    GCTTGGTCTA GATCAGACAG TGACGAGGTG CCTCCAAGGG TTCCCGTGAG AACGACCTCT
    CGTTCCCCTG TCCTCTCCCG TCGGGATTCC CCACTGCAGG GCGGTGGACA GCAGAATAGC
    CAAGCAGGAC AGAGAAATTC CACCAGCAGT ATTGAGCCCC GGCTTCTTTG GGAGAGAGTG
    GAAAAGCTGG TCCCCAGGCC AGGCAGTGGC AGCTCTTCAG GGTCCAGCAA CTCAGGATCC
    CAGCCTGGCT CCCATCCTGG GTCTCAGAGC GGCTCCGGTG AACGCTTCAG AGTGAGATCA
    TCATCCAAGT CTGAAGGTTC TCCATCGCCA CGCCAGGAAA GTGCAGCCAA AAAGCCTGAC
    GATAAGAAAG AAGTATTCCG GCCTCTCAAG CCTGCGGGAG AAGTGGACTT GACTGCGTTG
    GCCAAAGAGC TTCGAGCAGT GGAAGATGTT CGGCCACCTC ACAAAGTAAC AGACTACTCA
    TCCTCCAGTG AGGAGTCTGG GACCACAGAT GAGGAGGAGG AGGACGTGGA ACAGGAGGGG
    GCTGATGATT CTACCTCGGG ACCAGAAGAC ACCAGAGCAG CGTCATCTCC GAACCTGAGC
    AACGGTGAAA CAGAATCTGT GAAAACAATG ATCGTTCATG ATGATGTAGA AAGTGAGCCA
    GCCATGACCC CGTCCAAGGA GGGCACCCTC ATCGTCCGCC AGACTCAGTC CGCTAGTAGC
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGGTTACTA
    CAGATCTCTC CATCTAGTGG GACGACAGTG ACTTCCGTGG TGGGATTTTC CTGTGATGGG
    CTGAGACCAG AAGCCATAAG GCAAGATCCT ACTCGGAAAG GCTCAGTGGT CAATGTGAAC
    CCCACGAACA CTAGGCCACA GAGTGATACC CCCGAGATTC GTAAATACAA GAAGAGATTT
    AACTCAGAGA TCCTGTGTGC TGCCTTATGG GGAGTGAACT TGCTGGTTGG TACAGAGAGC
    GGTCTGATGC TCTTGGACAG AAGTGGCCAA GGGAAAGTAT ACCCTCTGAT CAGCCGAAGA
    CGGTTCCAGC AAATGGATGT GCTCGAAGGC CTAAATGTCC TGGTGACAAT ATCTGGCAAA
    AAGGATAAGT TACGTGTCTA CTATTTATCC TGGCTAAGAA ATAAGATTCT TCATAATGAT
    CCAGAGGTTG AGAAGAAGCA GGGGTGGACC ACTGTGGGCG ACTTGGAAGG GTGCGTGCAC
    TATAAAGTCG TAAAATATGA AAGAATCAAG TTTCTGGTAA TTGCTTTGAA GAGTTCTGTG
    GAAGTCTATG CATGGGCACC GAAGCCATAT CACAAATTTA TGGCCTTTAA GTCATTTGGA
    GAACTGCTAC ATAAGCCATT ACTGGTGGAT CTCACTGTGG AGGAAGGCCA GAGGTTGAAA
    GTGATCTATG GATCCTGTGC GGGATTCCAT GCTGTTGATG TGGATTCAGG CTCAGTCTAT
    GACATTTATC TACCAACACA TATTCAGTGT AGCATCAAAC CCCATGCAAT CATTATCCTC
    CCCAACACGG ATGGGATGGA GCTGCTGGTG TGCTACGAAG ACGAGGGGGT TTACGTTAAC
    ACGTACGGAA GGATCACCAA GGACGTGGTT CTGCAGTGGG GAGAGATGCC GACATCTGTA
    GCATATATCC GGTCGAATCA GACAATGGGC TGGGGAGAGA AGGCAATAGA GATACGATCT
    GTGGAAACTG GTCACTTGGA TGGTGTATTT ATGCACAAAA GGGCTCAGAG ACTAAAGTTC
    CTGTGTGAAC GCAATGACAA GGTGTTCTTT GCCTCCGTCC GCTCTGGTGG CAGCAGCCAG
    GTCTATTTCA TGACCTTAGG CAGGACTTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_032722.2
    CDS318..4022
    Translation

    Target ORF information:

    RefSeq Version NM_008696.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_008696.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT ACAGTGGTCC
    CACCTGGCAT CTCTCAAGAA CAATGTCTCC CCTGTCTCGA GATCCCATTC CTTCAGTGAC
    CCTTCTCCCA AATTCGCACA CCACCATCTC CGCTCTCAGG ACCCATGTCC ACCTTCCCGC
    AGTGAGGGGC TCAGTCAGAG CTCTGACTCT AAGTCGGAGG TGCCCGAGCC CACCCAAAAG
    GCTTGGTCTA GATCAGACAG TGACGAGGTG CCTCCAAGGG TTCCCGTGAG AACGACCTCT
    CGTTCCCCTG TCCTCTCCCG TCGGGATTCC CCACTGCAGG GCGGTGGACA GCAGAATAGC
    CAAGCAGGAC AGAGAAATTC CACCAGCAGT ATTGAGCCCC GGCTTCTTTG GGAGAGAGTG
    GAAAAGCTGG TCCCCAGGCC AGGCAGTGGC AGCTCTTCAG GGTCCAGCAA CTCAGGATCC
    CAGCCTGGCT CCCATCCTGG GTCTCAGAGC GGCTCCGGTG AACGCTTCAG AGTGAGATCA
    TCATCCAAGT CTGAAGGTTC TCCATCGCCA CGCCAGGAAA GTGCAGCCAA AAAGCCTGAC
    GATAAGAAAG AAGTATTCCG GCCTCTCAAG CCTGCGGGAG AAGTGGACTT GACTGCGTTG
    GCCAAAGAGC TTCGAGCAGT GGAAGATGTT CGGCCACCTC ACAAAGTAAC AGACTACTCA
    TCCTCCAGTG AGGAGTCTGG GACCACAGAT GAGGAGGAGG AGGACGTGGA ACAGGAGGGG
    GCTGATGATT CTACCTCGGG ACCAGAAGAC ACCAGAGCAG CGTCATCTCC GAACCTGAGC
    AACGGTGAAA CAGAATCTGT GAAAACAATG ATCGTTCATG ATGATGTAGA AAGTGAGCCA
    GCCATGACCC CGTCCAAGGA GGGCACCCTC ATCGTCCGCC AGACTCAGTC CGCTAGTAGC
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGGTTACTA
    CAGATCTCTC CATCTAGTGG GACGACAGTG ACTTCCGTGG TGGGATTTTC CTGTGATGGG
    CTGAGACCAG AAGCCATAAG GCAAGATCCT ACTCGGAAAG GCTCAGTGGT CAATGTGAAC
    CCCACGAACA CTAGGCCACA GAGTGATACC CCCGAGATTC GTAAATACAA GAAGAGATTT
    AACTCAGAGA TCCTGTGTGC TGCCTTATGG GGAGTGAACT TGCTGGTTGG TACAGAGAGC
    GGTCTGATGC TCTTGGACAG AAGTGGCCAA GGGAAAGTAT ACCCTCTGAT CAGCCGAAGA
    CGGTTCCAGC AAATGGATGT GCTCGAAGGC CTAAATGTCC TGGTGACAAT ATCTGGCAAA
    AAGGATAAGT TACGTGTCTA CTATTTATCC TGGCTAAGAA ATAAGATTCT TCATAATGAT
    CCAGAGGTTG AGAAGAAGCA GGGGTGGACC ACTGTGGGCG ACTTGGAAGG GTGCGTGCAC
    TATAAAGTCG TAAAATATGA AAGAATCAAG TTTCTGGTAA TTGCTTTGAA GAGTTCTGTG
    GAAGTCTATG CATGGGCACC GAAGCCATAT CACAAATTTA TGGCCTTTAA GTCATTTGGA
    GAACTGCTAC ATAAGCCATT ACTGGTGGAT CTCACTGTGG AGGAAGGCCA GAGGTTGAAA
    GTGATCTATG GATCCTGTGC GGGATTCCAT GCTGTTGATG TGGATTCAGG CTCAGTCTAT
    GACATTTATC TACCAACACA TATTCAGTGT AGCATCAAAC CCCATGCAAT CATTATCCTC
    CCCAACACGG ATGGGATGGA GCTGCTGGTG TGCTACGAAG ACGAGGGGGT TTACGTTAAC
    ACGTACGGAA GGATCACCAA GGACGTGGTT CTGCAGTGGG GAGAGATGCC GACATCTGTA
    GCATATATCC GGTCGAATCA GACAATGGGC TGGGGAGAGA AGGCAATAGA GATACGATCT
    GTGGAAACTG GTCACTTGGA TGGTGTATTT ATGCACAAAA GGGCTCAGAG ACTAAAGTTC
    CTGTGTGAAC GCAATGACAA GGTGTTCTTT GCCTCCGTCC GCTCTGGTGG CAGCAGCCAG
    GTCTATTTCA TGACCTTAGG CAGGACTTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16105
    Clone ID Related Accession (Same CDS sequence) NM_001252202.1
    Accession Version NM_001252202.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ142598.1, BB651050.1, BC145390.1, U88984.1, BC145391.1, AV445090.1, BQ571658.1, AK159799.1 and BF721981.1. Transcript Variant: This variant (4) lacks multiple in-frame exons in the central coding region, compared to variant 1, and encodes a shorter isoform (4), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145391.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTTCCCGTGA GAACGACCTC TCGTTCCCCT
    GTCCTCTCCC GTCGGGATTC CCCACTGCAG GGCGGTGGAC AGCAGAATAG CCAAGCAGGA
    CAGAGAAATT CCACCAGCAG TATTGAGCCC CGGCTTCTTT GGGAGAGAGT GGAAAAGCTG
    GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA GGGTCCAGCA ACTCAGGATC CCAGCCTGGC
    TCCCATCCTG GGTCTCAGAG CGGCTCCGGT GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGTT CTCCATCGCC ACGCCAGGAA AGTGCAGCCA AAAAGCCTGA CGATAAGAAA
    GAAGTATTCC GGCCTCTCAA GCCTGCGGAC TTGACTGCGT TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TTCGGCCACC TCACAAAGTA ACAGACTACT CATCCTCCAG TGAGGAGTCT
    GGGACCACAG ATGAGGAGGA GGAGGACGTG GAACAGGAGG GGGCTGATGA TTCTACCTCG
    GGACCAGAAG ACACCAGAGC AGCGTCATCT CCGAACCTGA GCAACGGTGA AACAGAATCT
    GTGAAAACAA TGATCGTTCA TGATGATGTA GAAAGTGAGC CAGCCATGAC CCCGTCCAAG
    GAGGGCACCC TCATCGTCCG CCAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGGTTAC TACAGATCTC TCCATCTAGT
    GGGACGACAG TGACTTCCGT GGTGGGATTT TCCTGTGATG GGCTGAGACC AGAAGCCATA
    AGGCAAGATC CTACTCGGAA AGGCTCAGTG GTCAATGTGA ACCCCACGAA CACTAGGCCA
    CAGAGTGATA CCCCCGAGAT TCGTAAATAC AAGAAGAGAT TTAACTCAGA GATCCTGTGT
    GCTGCCTTAT GGGGAGTGAA CTTGCTGGTT GGTACAGAGA GCGGTCTGAT GCTCTTGGAC
    AGAAGTGGCC AAGGGAAAGT ATACCCTCTG ATCAGCCGAA GACGGTTCCA GCAAATGGAT
    GTGCTCGAAG GCCTAAATGT CCTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC
    TACTATTTAT CCTGGCTAAG AAATAAGATT CTTCATAATG ATCCAGAGGT TGAGAAGAAG
    CAGGGGTGGA CCACTGTGGG CGACTTGGAA GGGTGCGTGC ACTATAAAGT CGTAAAATAT
    GAAAGAATCA AGTTTCTGGT AATTGCTTTG AAGAGTTCTG TGGAAGTCTA TGCATGGGCA
    CCGAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAACTGCT ACATAAGCCA
    TTACTGGTGG ATCTCACTGT GGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT
    GCGGGATTCC ATGCTGTTGA TGTGGATTCA GGCTCAGTCT ATGACATTTA TCTACCAACA
    CATATTCAGT GTAGCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGGATG
    GAGCTGCTGG TGTGCTACGA AGACGAGGGG GTTTACGTTA ACACGTACGG AAGGATCACC
    AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCGACATCTG TAGCATATAT CCGGTCGAAT
    CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGAT CTGTGGAAAC TGGTCACTTG
    GATGGTGTAT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGCAATGAC
    AAGGTGTTCT TTGCCTCCGT CCGCTCTGGT GGCAGCAGCC AGGTCTATTT CATGACCTTA
    GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001239131.1
    CDS318..3944
    Translation

    Target ORF information:

    RefSeq Version NM_001252202.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001252202.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTTCCCGTGA GAACGACCTC TCGTTCCCCT
    GTCCTCTCCC GTCGGGATTC CCCACTGCAG GGCGGTGGAC AGCAGAATAG CCAAGCAGGA
    CAGAGAAATT CCACCAGCAG TATTGAGCCC CGGCTTCTTT GGGAGAGAGT GGAAAAGCTG
    GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA GGGTCCAGCA ACTCAGGATC CCAGCCTGGC
    TCCCATCCTG GGTCTCAGAG CGGCTCCGGT GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGTT CTCCATCGCC ACGCCAGGAA AGTGCAGCCA AAAAGCCTGA CGATAAGAAA
    GAAGTATTCC GGCCTCTCAA GCCTGCGGAC TTGACTGCGT TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TTCGGCCACC TCACAAAGTA ACAGACTACT CATCCTCCAG TGAGGAGTCT
    GGGACCACAG ATGAGGAGGA GGAGGACGTG GAACAGGAGG GGGCTGATGA TTCTACCTCG
    GGACCAGAAG ACACCAGAGC AGCGTCATCT CCGAACCTGA GCAACGGTGA AACAGAATCT
    GTGAAAACAA TGATCGTTCA TGATGATGTA GAAAGTGAGC CAGCCATGAC CCCGTCCAAG
    GAGGGCACCC TCATCGTCCG CCAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGGTTAC TACAGATCTC TCCATCTAGT
    GGGACGACAG TGACTTCCGT GGTGGGATTT TCCTGTGATG GGCTGAGACC AGAAGCCATA
    AGGCAAGATC CTACTCGGAA AGGCTCAGTG GTCAATGTGA ACCCCACGAA CACTAGGCCA
    CAGAGTGATA CCCCCGAGAT TCGTAAATAC AAGAAGAGAT TTAACTCAGA GATCCTGTGT
    GCTGCCTTAT GGGGAGTGAA CTTGCTGGTT GGTACAGAGA GCGGTCTGAT GCTCTTGGAC
    AGAAGTGGCC AAGGGAAAGT ATACCCTCTG ATCAGCCGAA GACGGTTCCA GCAAATGGAT
    GTGCTCGAAG GCCTAAATGT CCTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC
    TACTATTTAT CCTGGCTAAG AAATAAGATT CTTCATAATG ATCCAGAGGT TGAGAAGAAG
    CAGGGGTGGA CCACTGTGGG CGACTTGGAA GGGTGCGTGC ACTATAAAGT CGTAAAATAT
    GAAAGAATCA AGTTTCTGGT AATTGCTTTG AAGAGTTCTG TGGAAGTCTA TGCATGGGCA
    CCGAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAACTGCT ACATAAGCCA
    TTACTGGTGG ATCTCACTGT GGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT
    GCGGGATTCC ATGCTGTTGA TGTGGATTCA GGCTCAGTCT ATGACATTTA TCTACCAACA
    CATATTCAGT GTAGCATCAA ACCCCATGCA ATCATTATCC TCCCCAACAC GGATGGGATG
    GAGCTGCTGG TGTGCTACGA AGACGAGGGG GTTTACGTTA ACACGTACGG AAGGATCACC
    AAGGACGTGG TTCTGCAGTG GGGAGAGATG CCGACATCTG TAGCATATAT CCGGTCGAAT
    CAGACAATGG GCTGGGGAGA GAAGGCAATA GAGATACGAT CTGTGGAAAC TGGTCACTTG
    GATGGTGTAT TTATGCACAA AAGGGCTCAG AGACTAAAGT TCCTGTGTGA ACGCAATGAC
    AAGGTGTTCT TTGCCTCCGT CCGCTCTGGT GGCAGCAGCC AGGTCTATTT CATGACCTTA
    GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu16201
    Clone ID Related Accession (Same CDS sequence) NM_001252201.1
    Accession Version NM_001252201.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ142598.1, BB651050.1, BC145390.1, U88984.1, BC141109.1, AV445090.1, BQ571658.1, AK159799.1 and BF721981.1. Transcript Variant: This variant (3) has multiple differences in the presence and absence of in-frame exons in the central coding region, compared to variant 1, and encodes a shorter isoform (3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC141109.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT TCCCGTGAGA
    ACGACCTCTC GTTCCCCTGT CCTCTCCCGT CGGGATTCCC CACTGCAGGG CGGTGGACAG
    CAGAATAGCC AAGCAGGACA GAGAAATTCC ACCAGCAGTA TTGAGCCCCG GCTTCTTTGG
    GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC
    TCAGGATCCC AGCCTGGCTC CCATCCTGGG TCTCAGAGCG GCTCCGGTGA ACGCTTCAGA
    GTGAGATCAT CATCCAAGTC TGAAGGTTCT CCATCGCCAC GCCAGGAAAG TGCAGCCAAA
    AAGCCTGACG ATAAGAAAGA AGTATTCCGG CCTCTCAAGC CTGCGGGAGA AGTGGACTTG
    ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTTC GGCCACCTCA CAAAGTAACA
    GACTACTCAT CCTCCAGTGA GGAGTCTGGG ACCACAGATG AGGAGGAGGA GGACGTGGAA
    CAGGAGGGGG CTGATGATTC TACCTCGGGA CCAGAAGACA CCAGAGCAGC GTCATCTCCG
    AACCTGAGCA ACGGTGAAAC AGAATCTGTG AAAACAATGA TCGTTCATGA TGATGTAGAA
    AGTGAGCCAG CCATGACCCC GTCCAAGGAG GGCACCCTCA TCGTCCGCCA GAGTACAGTT
    GACCAAAAGC GTGCCAGCCA TCATGAGAGC AATGGCTTTG CGGGTCGCAT TCACCTCTTG
    CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCTTC CTCCCCATCC
    TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTGCTAAT
    GAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT
    TTTATAGACC CCAGGTTACT ACAGATCTCT CCATCTAGTG GGACGACAGT GACTTCCGTG
    GTGGGATTTT CCTGTGATGG GCTGAGACCA GAAGCCATAA GGCAAGATCC TACTCGGAAA
    GGCTCAGTGG TCAATGTGAA CCCCACGAAC ACTAGGCCAC AGAGTGATAC CCCCGAGATT
    CGTAAATACA AGAAGAGATT TAACTCAGAG ATCCTGTGTG CTGCCTTATG GGGAGTGAAC
    TTGCTGGTTG GTACAGAGAG CGGTCTGATG CTCTTGGACA GAAGTGGCCA AGGGAAAGTA
    TACCCTCTGA TCAGCCGAAG ACGGTTCCAG CAAATGGATG TGCTCGAAGG CCTAAATGTC
    CTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTATC CTGGCTAAGA
    AATAAGATTC TTCATAATGA TCCAGAGGTT GAGAAGAAGC AGGGGTGGAC CACTGTGGGC
    GACTTGGAAG GGTGCGTGCA CTATAAAGTC GTAAAATATG AAAGAATCAA GTTTCTGGTA
    ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT GCATGGGCAC CGAAGCCATA TCACAAATTT
    ATGGCCTTTA AGTCATTTGG AGAACTGCTA CATAAGCCAT TACTGGTGGA TCTCACTGTG
    GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CGGGATTCCA TGCTGTTGAT
    GTGGATTCAG GCTCAGTCTA TGACATTTAT CTACCAACAC ATATTCAGTG TAGCATCAAA
    CCCCATGCAA TCATTATCCT CCCCAACACG GATGGGATGG AGCTGCTGGT GTGCTACGAA
    GACGAGGGGG TTTACGTTAA CACGTACGGA AGGATCACCA AGGACGTGGT TCTGCAGTGG
    GGAGAGATGC CGACATCTGT AGCATATATC CGGTCGAATC AGACAATGGG CTGGGGAGAG
    AAGGCAATAG AGATACGATC TGTGGAAACT GGTCACTTGG ATGGTGTATT TATGCACAAA
    AGGGCTCAGA GACTAAAGTT CCTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCCGTC
    CGCTCTGGTG GCAGCAGCCA GGTCTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001239130.1
    CDS318..3983
    Translation

    Target ORF information:

    RefSeq Version NM_001252201.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001252201.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT TCCCGTGAGA
    ACGACCTCTC GTTCCCCTGT CCTCTCCCGT CGGGATTCCC CACTGCAGGG CGGTGGACAG
    CAGAATAGCC AAGCAGGACA GAGAAATTCC ACCAGCAGTA TTGAGCCCCG GCTTCTTTGG
    GAGAGAGTGG AAAAGCTGGT CCCCAGGCCA GGCAGTGGCA GCTCTTCAGG GTCCAGCAAC
    TCAGGATCCC AGCCTGGCTC CCATCCTGGG TCTCAGAGCG GCTCCGGTGA ACGCTTCAGA
    GTGAGATCAT CATCCAAGTC TGAAGGTTCT CCATCGCCAC GCCAGGAAAG TGCAGCCAAA
    AAGCCTGACG ATAAGAAAGA AGTATTCCGG CCTCTCAAGC CTGCGGGAGA AGTGGACTTG
    ACTGCGTTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTTC GGCCACCTCA CAAAGTAACA
    GACTACTCAT CCTCCAGTGA GGAGTCTGGG ACCACAGATG AGGAGGAGGA GGACGTGGAA
    CAGGAGGGGG CTGATGATTC TACCTCGGGA CCAGAAGACA CCAGAGCAGC GTCATCTCCG
    AACCTGAGCA ACGGTGAAAC AGAATCTGTG AAAACAATGA TCGTTCATGA TGATGTAGAA
    AGTGAGCCAG CCATGACCCC GTCCAAGGAG GGCACCCTCA TCGTCCGCCA GAGTACAGTT
    GACCAAAAGC GTGCCAGCCA TCATGAGAGC AATGGCTTTG CGGGTCGCAT TCACCTCTTG
    CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCTTC CTCCCCATCC
    TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTGCTAAT
    GAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT
    TTTATAGACC CCAGGTTACT ACAGATCTCT CCATCTAGTG GGACGACAGT GACTTCCGTG
    GTGGGATTTT CCTGTGATGG GCTGAGACCA GAAGCCATAA GGCAAGATCC TACTCGGAAA
    GGCTCAGTGG TCAATGTGAA CCCCACGAAC ACTAGGCCAC AGAGTGATAC CCCCGAGATT
    CGTAAATACA AGAAGAGATT TAACTCAGAG ATCCTGTGTG CTGCCTTATG GGGAGTGAAC
    TTGCTGGTTG GTACAGAGAG CGGTCTGATG CTCTTGGACA GAAGTGGCCA AGGGAAAGTA
    TACCCTCTGA TCAGCCGAAG ACGGTTCCAG CAAATGGATG TGCTCGAAGG CCTAAATGTC
    CTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTATC CTGGCTAAGA
    AATAAGATTC TTCATAATGA TCCAGAGGTT GAGAAGAAGC AGGGGTGGAC CACTGTGGGC
    GACTTGGAAG GGTGCGTGCA CTATAAAGTC GTAAAATATG AAAGAATCAA GTTTCTGGTA
    ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT GCATGGGCAC CGAAGCCATA TCACAAATTT
    ATGGCCTTTA AGTCATTTGG AGAACTGCTA CATAAGCCAT TACTGGTGGA TCTCACTGTG
    GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CGGGATTCCA TGCTGTTGAT
    GTGGATTCAG GCTCAGTCTA TGACATTTAT CTACCAACAC ATATTCAGTG TAGCATCAAA
    CCCCATGCAA TCATTATCCT CCCCAACACG GATGGGATGG AGCTGCTGGT GTGCTACGAA
    GACGAGGGGG TTTACGTTAA CACGTACGGA AGGATCACCA AGGACGTGGT TCTGCAGTGG
    GGAGAGATGC CGACATCTGT AGCATATATC CGGTCGAATC AGACAATGGG CTGGGGAGAG
    AAGGCAATAG AGATACGATC TGTGGAAACT GGTCACTTGG ATGGTGTATT TATGCACAAA
    AGGGCTCAGA GACTAAAGTT CCTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCCGTC
    CGCTCTGGTG GCAGCAGCCA GGTCTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27454
    Clone ID Related Accession (Same CDS sequence) XM_006496042.1 , XM_006496042.2
    Accession Version XM_006496042.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTACAGTGG TCCCACCTGG CATCTCTCAA GAACAATGTC
    TCCCCTGTCT CGAGATCCCA TTCCTTCAGT GACCCTTCTC CCAAATTCGC ACACCACCAT
    CTCCGCTCTC AGGACCCATG TCCACCTTCC CGCAGTGAGG GGCTCAGTCA GAGCTCTGAC
    TCTAAGTCGG AGGTGCCCGA GCCCACCCAA AAGGCTTGGT CTAGATCAGA CAGTGACGAG
    GTGCCTCCAA GGGTTCCCGT GAGAACGACC TCTCGTTCCC CTGTCCTCTC CCGTCGGGAT
    TCCCCACTGC AGGGCGGTGG ACAGCAGAAT AGCCAAGCAG GACAGAGAAA TTCCACCAGC
    AGTATTGAGC CCCGGCTTCT TTGGGAGAGA GTGGAAAAGC TGGTCCCCAG GCCAGGCAGT
    GGCAGCTCTT CAGGGTCCAG CAACTCAGGA TCCCAGCCTG GCTCCCATCC TGGGTCTCAG
    AGCGGCTCCG GTGAACGCTT CAGAGTGAGA TCATCATCCA AGTCTGAAGG TTCTCCATCG
    CCACGCCAGG AAAGTGCAGC CAAAAAGCCT GACGATAAGA AAGAAGTATT CCGGCCTCTC
    AAGCCTGCGG GAGAAGTGGA CTTGACTGCG TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTTCGGCCAC CTCACAAAGT AACAGACTAC TCATCCTCCA GTGAGGAGTC TGGGACCACA
    GATGAGGAGG AGGAGGACGT GGAACAGGAG GGGGCTGATG ATTCTACCTC GGGACCAGAA
    GACACCAGAG CAGCGTCATC TCCGAACCTG AGCAACGGTG AAACAGAATC TGTGAAAACA
    ATGATCGTTC ATGATGATGT AGAAAGTGAG CCAGCCATGA CCCCGTCCAA GGAGGGCACC
    CTCATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC
    TTTGCGGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CTTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGGT TACTACAGAT CTCTCCATCT
    AGTGGGACGA CAGTGACTTC CGTGGTGGGA TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACTCG GAAAGGCTCA GTGGTCAATG TGAACCCCAC GAACACTAGG
    CCACAGAGTG ATACCCCCGA GATTCGTAAA TACAAGAAGA GATTTAACTC AGAGATCCTG
    TGTGCTGCCT TATGGGGAGT GAACTTGCTG GTTGGTACAG AGAGCGGTCT GATGCTCTTG
    GACAGAAGTG GCCAAGGGAA AGTATACCCT CTGATCAGCC GAAGACGGTT CCAGCAAATG
    GATGTGCTCG AAGGCCTAAA TGTCCTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TATCCTGGCT AAGAAATAAG ATTCTTCATA ATGATCCAGA GGTTGAGAAG
    AAGCAGGGGT GGACCACTGT GGGCGACTTG GAAGGGTGCG TGCACTATAA AGTCGTAAAA
    TATGAAAGAA TCAAGTTTCT GGTAATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCATGG
    GCACCGAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAACT GCTACATAAG
    CCATTACTGG TGGATCTCAC TGTGGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCGGGAT TCCATGCTGT TGATGTGGAT TCAGGCTCAG TCTATGACAT TTATCTACCA
    ACACATATTC AGTGTAGCAT CAAACCCCAT GCAATCATTA TCCTCCCCAA CACGGATGGG
    ATGGAGCTGC TGGTGTGCTA CGAAGACGAG GGGGTTTACG TTAACACGTA CGGAAGGATC
    ACCAAGGACG TGGTTCTGCA GTGGGGAGAG ATGCCGACAT CTGTAGCATA TATCCGGTCG
    AATCAGACAA TGGGCTGGGG AGAGAAGGCA ATAGAGATAC GATCTGTGGA AACTGGTCAC
    TTGGATGGTG TATTTATGCA CAAAAGGGCT CAGAGACTAA AGTTCCTGTG TGAACGCAAT
    GACAAGGTGT TCTTTGCCTC CGTCCGCTCT GGTGGCAGCA GCCAGGTCTA TTTCATGACC
    TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496105.1
    CDS319..4308
    Translation

    Target ORF information:

    RefSeq Version XM_006496042.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496042.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTACAGTGG TCCCACCTGG CATCTCTCAA GAACAATGTC
    TCCCCTGTCT CGAGATCCCA TTCCTTCAGT GACCCTTCTC CCAAATTCGC ACACCACCAT
    CTCCGCTCTC AGGACCCATG TCCACCTTCC CGCAGTGAGG GGCTCAGTCA GAGCTCTGAC
    TCTAAGTCGG AGGTGCCCGA GCCCACCCAA AAGGCTTGGT CTAGATCAGA CAGTGACGAG
    GTGCCTCCAA GGGTTCCCGT GAGAACGACC TCTCGTTCCC CTGTCCTCTC CCGTCGGGAT
    TCCCCACTGC AGGGCGGTGG ACAGCAGAAT AGCCAAGCAG GACAGAGAAA TTCCACCAGC
    AGTATTGAGC CCCGGCTTCT TTGGGAGAGA GTGGAAAAGC TGGTCCCCAG GCCAGGCAGT
    GGCAGCTCTT CAGGGTCCAG CAACTCAGGA TCCCAGCCTG GCTCCCATCC TGGGTCTCAG
    AGCGGCTCCG GTGAACGCTT CAGAGTGAGA TCATCATCCA AGTCTGAAGG TTCTCCATCG
    CCACGCCAGG AAAGTGCAGC CAAAAAGCCT GACGATAAGA AAGAAGTATT CCGGCCTCTC
    AAGCCTGCGG GAGAAGTGGA CTTGACTGCG TTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTTCGGCCAC CTCACAAAGT AACAGACTAC TCATCCTCCA GTGAGGAGTC TGGGACCACA
    GATGAGGAGG AGGAGGACGT GGAACAGGAG GGGGCTGATG ATTCTACCTC GGGACCAGAA
    GACACCAGAG CAGCGTCATC TCCGAACCTG AGCAACGGTG AAACAGAATC TGTGAAAACA
    ATGATCGTTC ATGATGATGT AGAAAGTGAG CCAGCCATGA CCCCGTCCAA GGAGGGCACC
    CTCATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC
    TTTGCGGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CTTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGGT TACTACAGAT CTCTCCATCT
    AGTGGGACGA CAGTGACTTC CGTGGTGGGA TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACTCG GAAAGGCTCA GTGGTCAATG TGAACCCCAC GAACACTAGG
    CCACAGAGTG ATACCCCCGA GATTCGTAAA TACAAGAAGA GATTTAACTC AGAGATCCTG
    TGTGCTGCCT TATGGGGAGT GAACTTGCTG GTTGGTACAG AGAGCGGTCT GATGCTCTTG
    GACAGAAGTG GCCAAGGGAA AGTATACCCT CTGATCAGCC GAAGACGGTT CCAGCAAATG
    GATGTGCTCG AAGGCCTAAA TGTCCTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TATCCTGGCT AAGAAATAAG ATTCTTCATA ATGATCCAGA GGTTGAGAAG
    AAGCAGGGGT GGACCACTGT GGGCGACTTG GAAGGGTGCG TGCACTATAA AGTCGTAAAA
    TATGAAAGAA TCAAGTTTCT GGTAATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCATGG
    GCACCGAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAACT GCTACATAAG
    CCATTACTGG TGGATCTCAC TGTGGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCGGGAT TCCATGCTGT TGATGTGGAT TCAGGCTCAG TCTATGACAT TTATCTACCA
    ACACATATTC AGTGTAGCAT CAAACCCCAT GCAATCATTA TCCTCCCCAA CACGGATGGG
    ATGGAGCTGC TGGTGTGCTA CGAAGACGAG GGGGTTTACG TTAACACGTA CGGAAGGATC
    ACCAAGGACG TGGTTCTGCA GTGGGGAGAG ATGCCGACAT CTGTAGCATA TATCCGGTCG
    AATCAGACAA TGGGCTGGGG AGAGAAGGCA ATAGAGATAC GATCTGTGGA AACTGGTCAC
    TTGGATGGTG TATTTATGCA CAAAAGGGCT CAGAGACTAA AGTTCCTGTG TGAACGCAAT
    GACAAGGTGT TCTTTGCCTC CGTCCGCTCT GGTGGCAGCA GCCAGGTCTA TTTCATGACC
    TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27455
    Clone ID Related Accession (Same CDS sequence) XM_006496043.1 , XM_006496043.2
    Accession Version XM_006496043.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTTCCCGTGA GAACGACCTC TCGTTCCCCT
    GTCCTCTCCC GTCGGGATTC CCCACTGCAG GGCGGTGGAC AGCAGAATAG CCAAGCAGGA
    CAGAGAAATT CCACCAGCAG TATTGAGCCC CGGCTTCTTT GGGAGAGAGT GGAAAAGCTG
    GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA GGGTCCAGCA ACTCAGGATC CCAGCCTGGC
    TCCCATCCTG GGTCTCAGAG CGGCTCCGGT GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGTT CTCCATCGCC ACGCCAGGAA AGTGCAGCCA AAAAGCCTGA CGATAAGAAA
    GAAGTATTCC GGCCTCTCAA GCCTGCGGAC TTGACTGCGT TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TTCGGCCACC TCACAAAGTA ACAGACTACT CATCCTCCAG TGAGGAGTCT
    GGGACCACAG ATGAGGAGGA GGAGGACGTG GAACAGGAGG GGGCTGATGA TTCTACCTCG
    GGACCAGAAG ACACCAGAGC AGCGTCATCT CCGAACCTGA GCAACGGTGA AACAGAATCT
    GTGAAAACAA TGATCGTTCA TGATGATGTA GAAAGTGAGC CAGCCATGAC CCCGTCCAAG
    GAGGGCACCC TCATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCGGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC TTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGGTT ACTACAGATC
    TCTCCATCTA GTGGGACGAC AGTGACTTCC GTGGTGGGAT TTTCCTGTGA TGGGCTGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACTCGG AAAGGCTCAG TGGTCAATGT GAACCCCACG
    AACACTAGGC CACAGAGTGA TACCCCCGAG ATTCGTAAAT ACAAGAAGAG ATTTAACTCA
    GAGATCCTGT GTGCTGCCTT ATGGGGAGTG AACTTGCTGG TTGGTACAGA GAGCGGTCTG
    ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA GTATACCCTC TGATCAGCCG AAGACGGTTC
    CAGCAAATGG ATGTGCTCGA AGGCCTAAAT GTCCTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT ATCCTGGCTA AGAAATAAGA TTCTTCATAA TGATCCAGAG
    GTTGAGAAGA AGCAGGGGTG GACCACTGTG GGCGACTTGG AAGGGTGCGT GCACTATAAA
    GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG GTAATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCATGGG CACCGAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAACTG
    CTACATAAGC CATTACTGGT GGATCTCACT GTGGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCGGGATT CCATGCTGTT GATGTGGATT CAGGCTCAGT CTATGACATT
    TATCTACCAA CACATATTCA GTGTAGCATC AAACCCCATG CAATCATTAT CCTCCCCAAC
    ACGGATGGGA TGGAGCTGCT GGTGTGCTAC GAAGACGAGG GGGTTTACGT TAACACGTAC
    GGAAGGATCA CCAAGGACGT GGTTCTGCAG TGGGGAGAGA TGCCGACATC TGTAGCATAT
    ATCCGGTCGA ATCAGACAAT GGGCTGGGGA GAGAAGGCAA TAGAGATACG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT ATTTATGCAC AAAAGGGCTC AGAGACTAAA GTTCCTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCC GTCCGCTCTG GTGGCAGCAG CCAGGTCTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496106.1
    CDS319..4137
    Translation

    Target ORF information:

    RefSeq Version XM_006496043.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496043.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTTCCCGTGA GAACGACCTC TCGTTCCCCT
    GTCCTCTCCC GTCGGGATTC CCCACTGCAG GGCGGTGGAC AGCAGAATAG CCAAGCAGGA
    CAGAGAAATT CCACCAGCAG TATTGAGCCC CGGCTTCTTT GGGAGAGAGT GGAAAAGCTG
    GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA GGGTCCAGCA ACTCAGGATC CCAGCCTGGC
    TCCCATCCTG GGTCTCAGAG CGGCTCCGGT GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGTT CTCCATCGCC ACGCCAGGAA AGTGCAGCCA AAAAGCCTGA CGATAAGAAA
    GAAGTATTCC GGCCTCTCAA GCCTGCGGAC TTGACTGCGT TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TTCGGCCACC TCACAAAGTA ACAGACTACT CATCCTCCAG TGAGGAGTCT
    GGGACCACAG ATGAGGAGGA GGAGGACGTG GAACAGGAGG GGGCTGATGA TTCTACCTCG
    GGACCAGAAG ACACCAGAGC AGCGTCATCT CCGAACCTGA GCAACGGTGA AACAGAATCT
    GTGAAAACAA TGATCGTTCA TGATGATGTA GAAAGTGAGC CAGCCATGAC CCCGTCCAAG
    GAGGGCACCC TCATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCGGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC TTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGGTT ACTACAGATC
    TCTCCATCTA GTGGGACGAC AGTGACTTCC GTGGTGGGAT TTTCCTGTGA TGGGCTGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACTCGG AAAGGCTCAG TGGTCAATGT GAACCCCACG
    AACACTAGGC CACAGAGTGA TACCCCCGAG ATTCGTAAAT ACAAGAAGAG ATTTAACTCA
    GAGATCCTGT GTGCTGCCTT ATGGGGAGTG AACTTGCTGG TTGGTACAGA GAGCGGTCTG
    ATGCTCTTGG ACAGAAGTGG CCAAGGGAAA GTATACCCTC TGATCAGCCG AAGACGGTTC
    CAGCAAATGG ATGTGCTCGA AGGCCTAAAT GTCCTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT ATCCTGGCTA AGAAATAAGA TTCTTCATAA TGATCCAGAG
    GTTGAGAAGA AGCAGGGGTG GACCACTGTG GGCGACTTGG AAGGGTGCGT GCACTATAAA
    GTCGTAAAAT ATGAAAGAAT CAAGTTTCTG GTAATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCATGGG CACCGAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAACTG
    CTACATAAGC CATTACTGGT GGATCTCACT GTGGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCGGGATT CCATGCTGTT GATGTGGATT CAGGCTCAGT CTATGACATT
    TATCTACCAA CACATATTCA GTGTAGCATC AAACCCCATG CAATCATTAT CCTCCCCAAC
    ACGGATGGGA TGGAGCTGCT GGTGTGCTAC GAAGACGAGG GGGTTTACGT TAACACGTAC
    GGAAGGATCA CCAAGGACGT GGTTCTGCAG TGGGGAGAGA TGCCGACATC TGTAGCATAT
    ATCCGGTCGA ATCAGACAAT GGGCTGGGGA GAGAAGGCAA TAGAGATACG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT ATTTATGCAC AAAAGGGCTC AGAGACTAAA GTTCCTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCC GTCCGCTCTG GTGGCAGCAG CCAGGTCTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27456
    Clone ID Related Accession (Same CDS sequence) XM_006496044.1 , XM_006496044.2
    Accession Version XM_006496044.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTTCCCG TGAGAACGAC CTCTCGTTCC CCTGTCCTCT CCCGTCGGGA TTCCCCACTG
    CAGGGCGGTG GACAGCAGAA TAGCCAAGCA GGACAGAGAA ATTCCACCAG TATTGAGCCC
    CGGCTTCTTT GGGAGAGAGT GGAAAAGCTG GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA
    GGGTCCAGCA ACTCAGGATC CCAGCCTGGC TCCCATCCTG GGTCTCAGAG CGGCTCCGGT
    GAACGCTTCA GAGTGAGATC ATCATCCAAG TCTGAAGGTT CTCCATCGCC ACGCCAGGAA
    AGTGCAGCCA AAAAGCCTGA CGATAAGAAA GAAGTATTCC GGCCTCTCAA GCCTGCGGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TTCGGCCACC TCACAAAGTA
    ACAGACTACT CATCCTCCAG TGAGGAGTCT GGGACCACAG ATGAGGAGGA GGAGGACGTG
    GAACAGGAGG GGGCTGATGA TTCTACCTCG GGACCAGAAG ACACCAGAGC AGCGTCATCT
    CCGAACCTGA GCAACGGTGA AACAGAATCT GTGAAAACAA TGATCGTTCA TGATGATGTA
    GAAAGTGAGC CAGCCATGAC CCCGTCCAAG GAGGGCACCC TCATCGTCCG CCAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGGTTAC TACAGATCTC TCCATCTAGT GGGACGACAG TGACTTCCGT GGTGGGATTT
    TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG
    GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC
    AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA CTTGCTGGTT
    GGTACAGAGA GCGGTCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT ATACCCTCTG
    ATCAGCCGAA GACGGTTCCA GCAAATGGAT GTGCTCGAAG GCCTAAATGT CCTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTAT CCTGGCTAAG AAATAAGATT
    CTTCATAATG ATCCAGAGGT TGAGAAGAAG CAGGGGTGGA CCACTGTGGG CGACTTGGAA
    GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATTGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAACTGCT ACATAAGCCA TTACTGGTGG ATCTCACTGT GGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCGGGATTCC ATGCTGTTGA TGTGGATTCA
    GGCTCAGTCT ATGACATTTA TCTACCAACA CATATTCAGT GTAGCATCAA ACCCCATGCA
    ATCATTATCC TCCCCAACAC GGATGGGATG GAGCTGCTGG TGTGCTACGA AGACGAGGGG
    GTTTACGTTA ACACGTACGG AAGGATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG
    CCGACATCTG TAGCATATAT CCGGTCGAAT CAGACAATGG GCTGGGGAGA GAAGGCAATA
    GAGATACGAT CTGTGGAAAC TGGTCACTTG GATGGTGTAT TTATGCACAA AAGGGCTCAG
    AGACTAAAGT TCCTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCCGT CCGCTCTGGT
    GGCAGCAGCC AGGTCTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496107.1
    CDS319..4035
    Translation

    Target ORF information:

    RefSeq Version XM_006496044.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496044.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTTCCCG TGAGAACGAC CTCTCGTTCC CCTGTCCTCT CCCGTCGGGA TTCCCCACTG
    CAGGGCGGTG GACAGCAGAA TAGCCAAGCA GGACAGAGAA ATTCCACCAG TATTGAGCCC
    CGGCTTCTTT GGGAGAGAGT GGAAAAGCTG GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA
    GGGTCCAGCA ACTCAGGATC CCAGCCTGGC TCCCATCCTG GGTCTCAGAG CGGCTCCGGT
    GAACGCTTCA GAGTGAGATC ATCATCCAAG TCTGAAGGTT CTCCATCGCC ACGCCAGGAA
    AGTGCAGCCA AAAAGCCTGA CGATAAGAAA GAAGTATTCC GGCCTCTCAA GCCTGCGGAC
    TTGACTGCGT TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TTCGGCCACC TCACAAAGTA
    ACAGACTACT CATCCTCCAG TGAGGAGTCT GGGACCACAG ATGAGGAGGA GGAGGACGTG
    GAACAGGAGG GGGCTGATGA TTCTACCTCG GGACCAGAAG ACACCAGAGC AGCGTCATCT
    CCGAACCTGA GCAACGGTGA AACAGAATCT GTGAAAACAA TGATCGTTCA TGATGATGTA
    GAAAGTGAGC CAGCCATGAC CCCGTCCAAG GAGGGCACCC TCATCGTCCG CCAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGGTTAC TACAGATCTC TCCATCTAGT GGGACGACAG TGACTTCCGT GGTGGGATTT
    TCCTGTGATG GGCTGAGACC AGAAGCCATA AGGCAAGATC CTACTCGGAA AGGCTCAGTG
    GTCAATGTGA ACCCCACGAA CACTAGGCCA CAGAGTGATA CCCCCGAGAT TCGTAAATAC
    AAGAAGAGAT TTAACTCAGA GATCCTGTGT GCTGCCTTAT GGGGAGTGAA CTTGCTGGTT
    GGTACAGAGA GCGGTCTGAT GCTCTTGGAC AGAAGTGGCC AAGGGAAAGT ATACCCTCTG
    ATCAGCCGAA GACGGTTCCA GCAAATGGAT GTGCTCGAAG GCCTAAATGT CCTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTAT CCTGGCTAAG AAATAAGATT
    CTTCATAATG ATCCAGAGGT TGAGAAGAAG CAGGGGTGGA CCACTGTGGG CGACTTGGAA
    GGGTGCGTGC ACTATAAAGT CGTAAAATAT GAAAGAATCA AGTTTCTGGT AATTGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCATGGGCA CCGAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAACTGCT ACATAAGCCA TTACTGGTGG ATCTCACTGT GGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCGGGATTCC ATGCTGTTGA TGTGGATTCA
    GGCTCAGTCT ATGACATTTA TCTACCAACA CATATTCAGT GTAGCATCAA ACCCCATGCA
    ATCATTATCC TCCCCAACAC GGATGGGATG GAGCTGCTGG TGTGCTACGA AGACGAGGGG
    GTTTACGTTA ACACGTACGG AAGGATCACC AAGGACGTGG TTCTGCAGTG GGGAGAGATG
    CCGACATCTG TAGCATATAT CCGGTCGAAT CAGACAATGG GCTGGGGAGA GAAGGCAATA
    GAGATACGAT CTGTGGAAAC TGGTCACTTG GATGGTGTAT TTATGCACAA AAGGGCTCAG
    AGACTAAAGT TCCTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCCGT CCGCTCTGGT
    GGCAGCAGCC AGGTCTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27457
    Clone ID Related Accession (Same CDS sequence) XM_006496045.1 , XM_006496045.2
    Accession Version XM_006496045.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTTCCCGTGA GAACGACCTC TCGTTCCCCT
    GTCCTCTCCC GTCGGGATTC CCCACTGCAG GGCGGTGGAC AGCAGAATAG CCAAGCAGGA
    CAGAGAAATT CCACCAGCAG TATTGAGCCC CGGCTTCTTT GGGAGAGAGT GGAAAAGCTG
    GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA GGGTCCAGCA ACTCAGGATC CCAGCCTGGC
    TCCCATCCTG GGTCTCAGAG CGGCTCCGGT GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGTT CTCCATCGCC ACGCCAGGAA AGTGCAGCCA AAAAGCCTGA CGATAAGAAA
    GAAGTATTCC GGCCTCTCAA GCCTGCGGGA GAAGTGGACT TGACTGCGTT GGCCAAAGAG
    CTTCGAGCAG TGGAAGATGT TCGGCCACCT CACAAAGTAA CAGACTACTC ATCCTCCAGT
    GAGGAGTCTG GGACCACAGA TGAGGAGGAG GAGGACGTGG AACAGGAGGG GGCTGATGAT
    TCTACCTCGG GACCAGAAGA CACCAGAGCA GCGTCATCTC CGAACCTGAG CAACGGTGAA
    ACAGAATCTG TGAAAACAAT GATCGTTCAT GATGATGTAG AAAGTGAGCC AGCCATGACC
    CCGTCCAAGG AGGGCACCCT CATCGTCCGC CAGACTCAGT CCGCTAGTAG CACACTCCAG
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGGTTACT ACAGATCTCT
    CCATCTAGTG GGACGACAGT GACTTCCGTG GTGGGATTTT CCTGTGATGG GCTGAGACCA
    GAAGCCATAA GGCAAGATCC TACTCGGAAA GGCTCAGTGG TCAATGTGAA CCCCACGAAC
    ACTAGGCCAC AGAGTGATAC CCCCGAGATT CGTAAATACA AGAAGAGATT TAACTCAGAG
    ATCCTGTGTG CTGCCTTATG GGGAGTGAAC TTGCTGGTTG GTACAGAGAG CGGTCTGATG
    CTCTTGGACA GAAGTGGCCA AGGGAAAGTA TACCCTCTGA TCAGCCGAAG ACGGTTCCAG
    CAAATGGATG TGCTCGAAGG CCTAAATGTC CTGGTGACAA TATCTGGCAA AAAGGATAAG
    TTACGTGTCT ACTATTTATC CTGGCTAAGA AATAAGATTC TTCATAATGA TCCAGAGGTT
    GAGAAGAAGC AGGGGTGGAC CACTGTGGGC GACTTGGAAG GGTGCGTGCA CTATAAAGTC
    GTAAAATATG AAAGAATCAA GTTTCTGGTA ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT
    GCATGGGCAC CGAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAACTGCTA
    CATAAGCCAT TACTGGTGGA TCTCACTGTG GAGGAAGGCC AGAGGTTGAA AGTGATCTAT
    GGATCCTGTG CGGGATTCCA TGCTGTTGAT GTGGATTCAG GCTCAGTCTA TGACATTTAT
    CTACCAACAC ATATTCAGTG TAGCATCAAA CCCCATGCAA TCATTATCCT CCCCAACACG
    GATGGGATGG AGCTGCTGGT GTGCTACGAA GACGAGGGGG TTTACGTTAA CACGTACGGA
    AGGATCACCA AGGACGTGGT TCTGCAGTGG GGAGAGATGC CGACATCTGT AGCATATATC
    CGGTCGAATC AGACAATGGG CTGGGGAGAG AAGGCAATAG AGATACGATC TGTGGAAACT
    GGTCACTTGG ATGGTGTATT TATGCACAAA AGGGCTCAGA GACTAAAGTT CCTGTGTGAA
    CGCAATGACA AGGTGTTCTT TGCCTCCGTC CGCTCTGGTG GCAGCAGCCA GGTCTATTTC
    ATGACCTTAG GCAGGACTTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496108.1
    CDS319..3954
    Translation

    Target ORF information:

    RefSeq Version XM_006496045.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496045.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT GGAAGATAGA
    TTTAGAAAGA CTAACCACAG CTCCCCTGAG GCCCAGGCTA AGCAAACGGG CAGAGGACTG
    GAGCCACCCG TGCCTTCCCG GTCAGAGTCT TTTTCCAATG GCAACTCCGA GTCTGTGCAC
    CCTGCCCTGC AGAGACCAGC GGAGCCACAG GTTCCCGTGA GAACGACCTC TCGTTCCCCT
    GTCCTCTCCC GTCGGGATTC CCCACTGCAG GGCGGTGGAC AGCAGAATAG CCAAGCAGGA
    CAGAGAAATT CCACCAGCAG TATTGAGCCC CGGCTTCTTT GGGAGAGAGT GGAAAAGCTG
    GTCCCCAGGC CAGGCAGTGG CAGCTCTTCA GGGTCCAGCA ACTCAGGATC CCAGCCTGGC
    TCCCATCCTG GGTCTCAGAG CGGCTCCGGT GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGTT CTCCATCGCC ACGCCAGGAA AGTGCAGCCA AAAAGCCTGA CGATAAGAAA
    GAAGTATTCC GGCCTCTCAA GCCTGCGGGA GAAGTGGACT TGACTGCGTT GGCCAAAGAG
    CTTCGAGCAG TGGAAGATGT TCGGCCACCT CACAAAGTAA CAGACTACTC ATCCTCCAGT
    GAGGAGTCTG GGACCACAGA TGAGGAGGAG GAGGACGTGG AACAGGAGGG GGCTGATGAT
    TCTACCTCGG GACCAGAAGA CACCAGAGCA GCGTCATCTC CGAACCTGAG CAACGGTGAA
    ACAGAATCTG TGAAAACAAT GATCGTTCAT GATGATGTAG AAAGTGAGCC AGCCATGACC
    CCGTCCAAGG AGGGCACCCT CATCGTCCGC CAGACTCAGT CCGCTAGTAG CACACTCCAG
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGGTTACT ACAGATCTCT
    CCATCTAGTG GGACGACAGT GACTTCCGTG GTGGGATTTT CCTGTGATGG GCTGAGACCA
    GAAGCCATAA GGCAAGATCC TACTCGGAAA GGCTCAGTGG TCAATGTGAA CCCCACGAAC
    ACTAGGCCAC AGAGTGATAC CCCCGAGATT CGTAAATACA AGAAGAGATT TAACTCAGAG
    ATCCTGTGTG CTGCCTTATG GGGAGTGAAC TTGCTGGTTG GTACAGAGAG CGGTCTGATG
    CTCTTGGACA GAAGTGGCCA AGGGAAAGTA TACCCTCTGA TCAGCCGAAG ACGGTTCCAG
    CAAATGGATG TGCTCGAAGG CCTAAATGTC CTGGTGACAA TATCTGGCAA AAAGGATAAG
    TTACGTGTCT ACTATTTATC CTGGCTAAGA AATAAGATTC TTCATAATGA TCCAGAGGTT
    GAGAAGAAGC AGGGGTGGAC CACTGTGGGC GACTTGGAAG GGTGCGTGCA CTATAAAGTC
    GTAAAATATG AAAGAATCAA GTTTCTGGTA ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT
    GCATGGGCAC CGAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAACTGCTA
    CATAAGCCAT TACTGGTGGA TCTCACTGTG GAGGAAGGCC AGAGGTTGAA AGTGATCTAT
    GGATCCTGTG CGGGATTCCA TGCTGTTGAT GTGGATTCAG GCTCAGTCTA TGACATTTAT
    CTACCAACAC ATATTCAGTG TAGCATCAAA CCCCATGCAA TCATTATCCT CCCCAACACG
    GATGGGATGG AGCTGCTGGT GTGCTACGAA GACGAGGGGG TTTACGTTAA CACGTACGGA
    AGGATCACCA AGGACGTGGT TCTGCAGTGG GGAGAGATGC CGACATCTGT AGCATATATC
    CGGTCGAATC AGACAATGGG CTGGGGAGAG AAGGCAATAG AGATACGATC TGTGGAAACT
    GGTCACTTGG ATGGTGTATT TATGCACAAA AGGGCTCAGA GACTAAAGTT CCTGTGTGAA
    CGCAATGACA AGGTGTTCTT TGCCTCCGTC CGCTCTGGTG GCAGCAGCCA GGTCTATTTC
    ATGACCTTAG GCAGGACTTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27458
    Clone ID Related Accession (Same CDS sequence) XM_006496046.1 , XM_006496046.2
    Accession Version XM_006496046.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT TCCCGTGAGA
    ACGACCTCTC GTTCCCCTGT CCTCTCCCGT CGGGATTCCC CACTGCAGGG CGGTGGACAG
    CAGAATAGCC AAGCAGGACA GAGAAATTCC ACCAGTATTG AGCCCCGGCT TCTTTGGGAG
    AGAGTGGAAA AGCTGGTCCC CAGGCCAGGC AGTGGCAGCT CTTCAGGGTC CAGCAACTCA
    GGATCCCAGC CTGGCTCCCA TCCTGGGTCT CAGAGCGGCT CCGGTGAACG CTTCAGAGTG
    AGATCATCAT CCAAGTCTGA AGGTTCTCCA TCGCCACGCC AGGAAAGTGC AGCCAAAAAG
    CCTGACGATA AGAAAGAAGT ATTCCGGCCT CTCAAGCCTG CGGGAGAAGT GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTTCGGC CACCTCACAA AGTAACAGAC
    TACTCATCCT CCAGTGAGGA GTCTGGGACC ACAGATGAGG AGGAGGAGGA CGTGGAACAG
    GAGGGGGCTG ATGATTCTAC CTCGGGACCA GAAGACACCA GAGCAGCGTC ATCTCCGAAC
    CTGAGCAACG GTGAAACAGA ATCTGTGAAA ACAATGATCG TTCATGATGA TGTAGAAAGT
    GAGCCAGCCA TGACCCCGTC CAAGGAGGGC ACCCTCATCG TCCGCCAGAC TCAGTCCGCT
    AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGG
    TTACTACAGA TCTCTCCATC TAGTGGGACG ACAGTGACTT CCGTGGTGGG ATTTTCCTGT
    GATGGGCTGA GACCAGAAGC CATAAGGCAA GATCCTACTC GGAAAGGCTC AGTGGTCAAT
    GTGAACCCCA CGAACACTAG GCCACAGAGT GATACCCCCG AGATTCGTAA ATACAAGAAG
    AGATTTAACT CAGAGATCCT GTGTGCTGCC TTATGGGGAG TGAACTTGCT GGTTGGTACA
    GAGAGCGGTC TGATGCTCTT GGACAGAAGT GGCCAAGGGA AAGTATACCC TCTGATCAGC
    CGAAGACGGT TCCAGCAAAT GGATGTGCTC GAAGGCCTAA ATGTCCTGGT GACAATATCT
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTATCCTGGC TAAGAAATAA GATTCTTCAT
    AATGATCCAG AGGTTGAGAA GAAGCAGGGG TGGACCACTG TGGGCGACTT GGAAGGGTGC
    GTGCACTATA AAGTCGTAAA ATATGAAAGA ATCAAGTTTC TGGTAATTGC TTTGAAGAGT
    TCTGTGGAAG TCTATGCATG GGCACCGAAG CCATATCACA AATTTATGGC CTTTAAGTCA
    TTTGGAGAAC TGCTACATAA GCCATTACTG GTGGATCTCA CTGTGGAGGA AGGCCAGAGG
    TTGAAAGTGA TCTATGGATC CTGTGCGGGA TTCCATGCTG TTGATGTGGA TTCAGGCTCA
    GTCTATGACA TTTATCTACC AACACATATT CAGTGTAGCA TCAAACCCCA TGCAATCATT
    ATCCTCCCCA ACACGGATGG GATGGAGCTG CTGGTGTGCT ACGAAGACGA GGGGGTTTAC
    GTTAACACGT ACGGAAGGAT CACCAAGGAC GTGGTTCTGC AGTGGGGAGA GATGCCGACA
    TCTGTAGCAT ATATCCGGTC GAATCAGACA ATGGGCTGGG GAGAGAAGGC AATAGAGATA
    CGATCTGTGG AAACTGGTCA CTTGGATGGT GTATTTATGC ACAAAAGGGC TCAGAGACTA
    AAGTTCCTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CCGTCCGCTC TGGTGGCAGC
    AGCCAGGTCT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496109.1
    CDS319..3789
    Translation

    Target ORF information:

    RefSeq Version XM_006496046.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496046.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCACGACCA CAGGAGGCCG
    CACGCACAGC AGCAGCCGCC GCCCCCGCAG CAGCAGGACA GGAGCAAACC GAGCTTTCAT
    GCTCCAGAGC CCAAGCCTCA CTATGACCCT GCTGACAGAG CTCGGGAGGT TCCCGTGAGA
    ACGACCTCTC GTTCCCCTGT CCTCTCCCGT CGGGATTCCC CACTGCAGGG CGGTGGACAG
    CAGAATAGCC AAGCAGGACA GAGAAATTCC ACCAGTATTG AGCCCCGGCT TCTTTGGGAG
    AGAGTGGAAA AGCTGGTCCC CAGGCCAGGC AGTGGCAGCT CTTCAGGGTC CAGCAACTCA
    GGATCCCAGC CTGGCTCCCA TCCTGGGTCT CAGAGCGGCT CCGGTGAACG CTTCAGAGTG
    AGATCATCAT CCAAGTCTGA AGGTTCTCCA TCGCCACGCC AGGAAAGTGC AGCCAAAAAG
    CCTGACGATA AGAAAGAAGT ATTCCGGCCT CTCAAGCCTG CGGGAGAAGT GGACTTGACT
    GCGTTGGCCA AAGAGCTTCG AGCAGTGGAA GATGTTCGGC CACCTCACAA AGTAACAGAC
    TACTCATCCT CCAGTGAGGA GTCTGGGACC ACAGATGAGG AGGAGGAGGA CGTGGAACAG
    GAGGGGGCTG ATGATTCTAC CTCGGGACCA GAAGACACCA GAGCAGCGTC ATCTCCGAAC
    CTGAGCAACG GTGAAACAGA ATCTGTGAAA ACAATGATCG TTCATGATGA TGTAGAAAGT
    GAGCCAGCCA TGACCCCGTC CAAGGAGGGC ACCCTCATCG TCCGCCAGAC TCAGTCCGCT
    AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGG
    TTACTACAGA TCTCTCCATC TAGTGGGACG ACAGTGACTT CCGTGGTGGG ATTTTCCTGT
    GATGGGCTGA GACCAGAAGC CATAAGGCAA GATCCTACTC GGAAAGGCTC AGTGGTCAAT
    GTGAACCCCA CGAACACTAG GCCACAGAGT GATACCCCCG AGATTCGTAA ATACAAGAAG
    AGATTTAACT CAGAGATCCT GTGTGCTGCC TTATGGGGAG TGAACTTGCT GGTTGGTACA
    GAGAGCGGTC TGATGCTCTT GGACAGAAGT GGCCAAGGGA AAGTATACCC TCTGATCAGC
    CGAAGACGGT TCCAGCAAAT GGATGTGCTC GAAGGCCTAA ATGTCCTGGT GACAATATCT
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTATCCTGGC TAAGAAATAA GATTCTTCAT
    AATGATCCAG AGGTTGAGAA GAAGCAGGGG TGGACCACTG TGGGCGACTT GGAAGGGTGC
    GTGCACTATA AAGTCGTAAA ATATGAAAGA ATCAAGTTTC TGGTAATTGC TTTGAAGAGT
    TCTGTGGAAG TCTATGCATG GGCACCGAAG CCATATCACA AATTTATGGC CTTTAAGTCA
    TTTGGAGAAC TGCTACATAA GCCATTACTG GTGGATCTCA CTGTGGAGGA AGGCCAGAGG
    TTGAAAGTGA TCTATGGATC CTGTGCGGGA TTCCATGCTG TTGATGTGGA TTCAGGCTCA
    GTCTATGACA TTTATCTACC AACACATATT CAGTGTAGCA TCAAACCCCA TGCAATCATT
    ATCCTCCCCA ACACGGATGG GATGGAGCTG CTGGTGTGCT ACGAAGACGA GGGGGTTTAC
    GTTAACACGT ACGGAAGGAT CACCAAGGAC GTGGTTCTGC AGTGGGGAGA GATGCCGACA
    TCTGTAGCAT ATATCCGGTC GAATCAGACA ATGGGCTGGG GAGAGAAGGC AATAGAGATA
    CGATCTGTGG AAACTGGTCA CTTGGATGGT GTATTTATGC ACAAAAGGGC TCAGAGACTA
    AAGTTCCTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CCGTCCGCTC TGGTGGCAGC
    AGCCAGGTCT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu69246
    Clone ID Related Accession (Same CDS sequence) XM_011238530.1 , XM_011238530.2
    Accession Version XM_011238530.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3960bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GCAGTATTGA GCCCCGGCTT
    CTTTGGGAGA GAGTGGAAAA GCTGGTCCCC AGGCCAGGCA GTGGCAGCTC TTCAGGGTCC
    AGCAACTCAG GATCCCAGCC TGGCTCCCAT CCTGGGTCTC AGAGCGGCTC CGGTGAACGC
    TTCAGAGTGA GATCATCATC CAAGTCTGAA GGTTCTCCAT CGCCACGCCA GGAAAGTGCA
    GCCAAAAAGC CTGACGATAA GAAAGAAGTA TTCCGGCCTC TCAAGCCTGC GGGAGAAGTG
    GACTTGACTG CGTTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTTCGGCC ACCTCACAAA
    GTAACAGACT ACTCATCCTC CAGTGAGGAG TCTGGGACCA CAGATGAGGA GGAGGAGGAC
    GTGGAACAGG AGGGGGCTGA TGATTCTACC TCGGGACCAG AAGACACCAG AGCAGCGTCA
    TCTCCGAACC TGAGCAACGG TGAAACAGAA TCTGTGAAAA CAATGATCGT TCATGATGAT
    GTAGAAAGTG AGCCAGCCAT GACCCCGTCC AAGGAGGGCA CCCTCATCGT CCGCCAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGGT TACTACAGAT CTCTCCATCT AGTGGGACGA CAGTGACTTC CGTGGTGGGA
    TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC ATAAGGCAAG ATCCTACTCG GAAAGGCTCA
    GTGGTCAATG TGAACCCCAC GAACACTAGG CCACAGAGTG ATACCCCCGA GATTCGTAAA
    TACAAGAAGA GATTTAACTC AGAGATCCTG TGTGCTGCCT TATGGGGAGT GAACTTGCTG
    GTTGGTACAG AGAGCGGTCT GATGCTCTTG GACAGAAGTG GCCAAGGGAA AGTATACCCT
    CTGATCAGCC GAAGACGGTT CCAGCAAATG GATGTGCTCG AAGGCCTAAA TGTCCTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TATCCTGGCT AAGAAATAAG
    ATTCTTCATA ATGATCCAGA GGTTGAGAAG AAGCAGGGGT GGACCACTGT GGGCGACTTG
    GAAGGGTGCG TGCACTATAA AGTCGTAAAA TATGAAAGAA TCAAGTTTCT GGTAATTGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCATGG GCACCGAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAACT GCTACATAAG CCATTACTGG TGGATCTCAC TGTGGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCGGGAT TCCATGCTGT TGATGTGGAT
    TCAGGCTCAG TCTATGACAT TTATCTACCA ACACATATTC AGTGTAGCAT CAAACCCCAT
    GCAATCATTA TCCTCCCCAA CACGGATGGG ATGGAGCTGC TGGTGTGCTA CGAAGACGAG
    GGGGTTTACG TTAACACGTA CGGAAGGATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG
    ATGCCGACAT CTGTAGCATA TATCCGGTCG AATCAGACAA TGGGCTGGGG AGAGAAGGCA
    ATAGAGATAC GATCTGTGGA AACTGGTCAC TTGGATGGTG TATTTATGCA CAAAAGGGCT
    CAGAGACTAA AGTTCCTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC CGTCCGCTCT
    GGTGGCAGCA GCCAGGTCTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011236832.1
    CDS319..4278
    Translation

    Target ORF information:

    RefSeq Version XM_011238530.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011238530.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GCAGTATTGA GCCCCGGCTT
    CTTTGGGAGA GAGTGGAAAA GCTGGTCCCC AGGCCAGGCA GTGGCAGCTC TTCAGGGTCC
    AGCAACTCAG GATCCCAGCC TGGCTCCCAT CCTGGGTCTC AGAGCGGCTC CGGTGAACGC
    TTCAGAGTGA GATCATCATC CAAGTCTGAA GGTTCTCCAT CGCCACGCCA GGAAAGTGCA
    GCCAAAAAGC CTGACGATAA GAAAGAAGTA TTCCGGCCTC TCAAGCCTGC GGGAGAAGTG
    GACTTGACTG CGTTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTTCGGCC ACCTCACAAA
    GTAACAGACT ACTCATCCTC CAGTGAGGAG TCTGGGACCA CAGATGAGGA GGAGGAGGAC
    GTGGAACAGG AGGGGGCTGA TGATTCTACC TCGGGACCAG AAGACACCAG AGCAGCGTCA
    TCTCCGAACC TGAGCAACGG TGAAACAGAA TCTGTGAAAA CAATGATCGT TCATGATGAT
    GTAGAAAGTG AGCCAGCCAT GACCCCGTCC AAGGAGGGCA CCCTCATCGT CCGCCAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGGT TACTACAGAT CTCTCCATCT AGTGGGACGA CAGTGACTTC CGTGGTGGGA
    TTTTCCTGTG ATGGGCTGAG ACCAGAAGCC ATAAGGCAAG ATCCTACTCG GAAAGGCTCA
    GTGGTCAATG TGAACCCCAC GAACACTAGG CCACAGAGTG ATACCCCCGA GATTCGTAAA
    TACAAGAAGA GATTTAACTC AGAGATCCTG TGTGCTGCCT TATGGGGAGT GAACTTGCTG
    GTTGGTACAG AGAGCGGTCT GATGCTCTTG GACAGAAGTG GCCAAGGGAA AGTATACCCT
    CTGATCAGCC GAAGACGGTT CCAGCAAATG GATGTGCTCG AAGGCCTAAA TGTCCTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TATCCTGGCT AAGAAATAAG
    ATTCTTCATA ATGATCCAGA GGTTGAGAAG AAGCAGGGGT GGACCACTGT GGGCGACTTG
    GAAGGGTGCG TGCACTATAA AGTCGTAAAA TATGAAAGAA TCAAGTTTCT GGTAATTGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCATGG GCACCGAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAACT GCTACATAAG CCATTACTGG TGGATCTCAC TGTGGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCGGGAT TCCATGCTGT TGATGTGGAT
    TCAGGCTCAG TCTATGACAT TTATCTACCA ACACATATTC AGTGTAGCAT CAAACCCCAT
    GCAATCATTA TCCTCCCCAA CACGGATGGG ATGGAGCTGC TGGTGTGCTA CGAAGACGAG
    GGGGTTTACG TTAACACGTA CGGAAGGATC ACCAAGGACG TGGTTCTGCA GTGGGGAGAG
    ATGCCGACAT CTGTAGCATA TATCCGGTCG AATCAGACAA TGGGCTGGGG AGAGAAGGCA
    ATAGAGATAC GATCTGTGGA AACTGGTCAC TTGGATGGTG TATTTATGCA CAAAAGGGCT
    CAGAGACTAA AGTTCCTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC CGTCCGCTCT
    GGTGGCAGCA GCCAGGTCTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu27453
    Clone ID Related Accession (Same CDS sequence) XM_006496041.1 , XM_006496041.2
    Accession Version XM_006496041.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GCAGTATTGA GCCCCGGCTT
    CTTTGGGAGA GAGTGGAAAA GCTGGTCCCC AGGCCAGGCA GTGGCAGCTC TTCAGGGTCC
    AGCAACTCAG GATCCCAGCC TGGCTCCCAT CCTGGGTCTC AGAGCGGCTC CGGTGAACGC
    TTCAGAGTGA GATCATCATC CAAGTCTGAA GGTTCTCCAT CGCCACGCCA GGAAAGTGCA
    GCCAAAAAGC CTGACGATAA GAAAGAAGTA TTCCGGCCTC TCAAGCCTGC GGGAGAAGTG
    GACTTGACTG CGTTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTTCGGCC ACCTCACAAA
    GTAACAGACT ACTCATCCTC CAGTGAGGAG TCTGGGACCA CAGATGAGGA GGAGGAGGAC
    GTGGAACAGG AGGGGGCTGA TGATTCTACC TCGGGACCAG AAGACACCAG AGCAGCGTCA
    TCTCCGAACC TGAGCAACGG TGAAACAGAA TCTGTGAAAA CAATGATCGT TCATGATGAT
    GTAGAAAGTG AGCCAGCCAT GACCCCGTCC AAGGAGGGCA CCCTCATCGT CCGCCAGAGT
    ACAGTTGACC AAAAGCGTGC CAGCCATCAT GAGAGCAATG GCTTTGCGGG TCGCATTCAC
    CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCTTCCTCC
    CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTCACT
    GCTAATGAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT
    ACACCTTTTA TAGACCCCAG GTTACTACAG ATCTCTCCAT CTAGTGGGAC GACAGTGACT
    TCCGTGGTGG GATTTTCCTG TGATGGGCTG AGACCAGAAG CCATAAGGCA AGATCCTACT
    CGGAAAGGCT CAGTGGTCAA TGTGAACCCC ACGAACACTA GGCCACAGAG TGATACCCCC
    GAGATTCGTA AATACAAGAA GAGATTTAAC TCAGAGATCC TGTGTGCTGC CTTATGGGGA
    GTGAACTTGC TGGTTGGTAC AGAGAGCGGT CTGATGCTCT TGGACAGAAG TGGCCAAGGG
    AAAGTATACC CTCTGATCAG CCGAAGACGG TTCCAGCAAA TGGATGTGCT CGAAGGCCTA
    AATGTCCTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTATCCTGG
    CTAAGAAATA AGATTCTTCA TAATGATCCA GAGGTTGAGA AGAAGCAGGG GTGGACCACT
    GTGGGCGACT TGGAAGGGTG CGTGCACTAT AAAGTCGTAA AATATGAAAG AATCAAGTTT
    CTGGTAATTG CTTTGAAGAG TTCTGTGGAA GTCTATGCAT GGGCACCGAA GCCATATCAC
    AAATTTATGG CCTTTAAGTC ATTTGGAGAA CTGCTACATA AGCCATTACT GGTGGATCTC
    ACTGTGGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCGGG ATTCCATGCT
    GTTGATGTGG ATTCAGGCTC AGTCTATGAC ATTTATCTAC CAACACATAT TCAGTGTAGC
    ATCAAACCCC ATGCAATCAT TATCCTCCCC AACACGGATG GGATGGAGCT GCTGGTGTGC
    TACGAAGACG AGGGGGTTTA CGTTAACACG TACGGAAGGA TCACCAAGGA CGTGGTTCTG
    CAGTGGGGAG AGATGCCGAC ATCTGTAGCA TATATCCGGT CGAATCAGAC AATGGGCTGG
    GGAGAGAAGG CAATAGAGAT ACGATCTGTG GAAACTGGTC ACTTGGATGG TGTATTTATG
    CACAAAAGGG CTCAGAGACT AAAGTTCCTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC
    TCCGTCCGCT CTGGTGGCAG CAGCCAGGTC TATTTCATGA CCTTAGGCAG GACTTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006496104.1
    CDS319..4470
    Translation

    Target ORF information:

    RefSeq Version XM_006496041.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006496041.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCTCCCGC GAAGAGCCTG GTGGACATTG ACCTGTCGTC CCTGCGGGAC 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGCTG GCCGCCATCA AGGTTATGGA CGTCACCGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTGA AGAAGTATTC TCATCATCGA
    AATATTGCCA CGTACTATGG TGCTTTCATT AAGAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT TTGTGGGGCT GGGTCCATCA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CTCTCAAAGA AGACTGGATT GCTTACATCT CCAGGGAAAT CCTCAGGGGA
    TTGGCACATC TCCATATTCA CCACGTTATT CACCGAGATA TCAAGGGCCA AAATGTGCTG
    CTGACCGAGA ATGCTGAGGT GAAACTTGTT GATTTTGGTG TAAGCGCTCA GCTGGACAGG
    ACGGTTGGAC GGAGAAATAC GTTCATAGGC ACACCCTACT GGATGGCTCC AGAGGTCATC
    GCCTGTGATG AGAACCCAGA CGCCACTTAC GACTACAGAA GTGACCTCTG GTCCTGTGGC
    ATCACAGCCA TCGAGATGGC TGAAGGGGCC CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCGCTGTTTC TCATCCCCAG AAACCCTCCT CCCAGGCTGA AGTCAAAAAA ATGGTCAAAG
    AAATTTTTCA GCTTTATAGA AGGCTGTCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAACTTT TAAAACACCC TTTCATAAGG GATCAGCCCA ATGAAAGGCA GGTTCGAATC
    CAGCTTAAGG ATCACATAGA CCGGACCAGA AAGAAGAGAG GCGAGAAAGA TGAGACGGAG
    TACGAGTACA GCGGGAGCGA GGAGGAGGAG GAGGAAGTGC CTGAGCAGGA GGGAGAGCCA
    AGTTCCATCG TCAATGTGCC TGGAGAGTCA ACTCTGCGAC GTGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAGCGGTC TGAGGCTCTG CGGAGACAGC AGCTTCTGCA GGAGCAGCAG
    CTCCGGGAGC AGGAGGAGTA TAAGAGGCAG CTGCTGGCTG AGAGGCAGAA GCGGATTGAA
    CAGCAGAAAG AACAGAGGAG GCGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCCAGG
    AGGCAGCAGG AGCGTGAGCA GCGGCGGCGT GAACAAGAGG AGAAGAGGCG TCTCGAGGAA
    CTGGAAAGGC GGCGTAAAGA AGAGGAAGAG AGGAGACGGG CAGAAGAGGA GAAGAGGAGA
    GTGGAGAGGG AACAGGAGTA CATCAGGCGG CAGCTAGAGG AGGAGCAGCG GCACCTGGAG
    ATCCTGCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCACGA CCACAGGAGG CCGCACGCAC AGCAGCAGCC GCCGCCCCCG
    CAGCAGCAGG ACAGGAGCAA ACCGAGCTTT CATGCTCCAG AGCCCAAGCC TCACTATGAC
    CCTGCTGACA GAGCTCGGGA GGTGGAAGAT AGATTTAGAA AGACTAACCA CAGCTCCCCT
    GAGGCCCAGG CTAAGCAAAC GGGCAGAGGA CTGGAGCCAC CCGTGCCTTC CCGGTCAGAG
    TCTTTTTCCA ATGGCAACTC CGAGTCTGTG CACCCTGCCC TGCAGAGACC AGCGGAGCCA
    CAGGTACAGT GGTCCCACCT GGCATCTCTC AAGAACAATG TCTCCCCTGT CTCGAGATCC
    CATTCCTTCA GTGACCCTTC TCCCAAATTC GCACACCACC ATCTCCGCTC TCAGGACCCA
    TGTCCACCTT CCCGCAGTGA GGGGCTCAGT CAGAGCTCTG ACTCTAAGTC GGAGGTGCCC
    GAGCCCACCC AAAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTTCCC
    GTGAGAACGA CCTCTCGTTC CCCTGTCCTC TCCCGTCGGG ATTCCCCACT GCAGGGCGGT
    GGACAGCAGA ATAGCCAAGC AGGACAGAGA AATTCCACCA GCAGTATTGA GCCCCGGCTT
    CTTTGGGAGA GAGTGGAAAA GCTGGTCCCC AGGCCAGGCA GTGGCAGCTC TTCAGGGTCC
    AGCAACTCAG GATCCCAGCC TGGCTCCCAT CCTGGGTCTC AGAGCGGCTC CGGTGAACGC
    TTCAGAGTGA GATCATCATC CAAGTCTGAA GGTTCTCCAT CGCCACGCCA GGAAAGTGCA
    GCCAAAAAGC CTGACGATAA GAAAGAAGTA TTCCGGCCTC TCAAGCCTGC GGGAGAAGTG
    GACTTGACTG CGTTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTTCGGCC ACCTCACAAA
    GTAACAGACT ACTCATCCTC CAGTGAGGAG TCTGGGACCA CAGATGAGGA GGAGGAGGAC
    GTGGAACAGG AGGGGGCTGA TGATTCTACC TCGGGACCAG AAGACACCAG AGCAGCGTCA
    TCTCCGAACC TGAGCAACGG TGAAACAGAA TCTGTGAAAA CAATGATCGT TCATGATGAT
    GTAGAAAGTG AGCCAGCCAT GACCCCGTCC AAGGAGGGCA CCCTCATCGT CCGCCAGAGT
    ACAGTTGACC AAAAGCGTGC CAGCCATCAT GAGAGCAATG GCTTTGCGGG TCGCATTCAC
    CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCTTCCTCC
    CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTCACT
    GCTAATGAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT
    ACACCTTTTA TAGACCCCAG GTTACTACAG ATCTCTCCAT CTAGTGGGAC GACAGTGACT
    TCCGTGGTGG GATTTTCCTG TGATGGGCTG AGACCAGAAG CCATAAGGCA AGATCCTACT
    CGGAAAGGCT CAGTGGTCAA TGTGAACCCC ACGAACACTA GGCCACAGAG TGATACCCCC
    GAGATTCGTA AATACAAGAA GAGATTTAAC TCAGAGATCC TGTGTGCTGC CTTATGGGGA
    GTGAACTTGC TGGTTGGTAC AGAGAGCGGT CTGATGCTCT TGGACAGAAG TGGCCAAGGG
    AAAGTATACC CTCTGATCAG CCGAAGACGG TTCCAGCAAA TGGATGTGCT CGAAGGCCTA
    AATGTCCTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTATCCTGG
    CTAAGAAATA AGATTCTTCA TAATGATCCA GAGGTTGAGA AGAAGCAGGG GTGGACCACT
    GTGGGCGACT TGGAAGGGTG CGTGCACTAT AAAGTCGTAA AATATGAAAG AATCAAGTTT
    CTGGTAATTG CTTTGAAGAG TTCTGTGGAA GTCTATGCAT GGGCACCGAA GCCATATCAC
    AAATTTATGG CCTTTAAGTC ATTTGGAGAA CTGCTACATA AGCCATTACT GGTGGATCTC
    ACTGTGGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCGGG ATTCCATGCT
    GTTGATGTGG ATTCAGGCTC AGTCTATGAC ATTTATCTAC CAACACATAT TCAGTGTAGC
    ATCAAACCCC ATGCAATCAT TATCCTCCCC AACACGGATG GGATGGAGCT GCTGGTGTGC
    TACGAAGACG AGGGGGTTTA CGTTAACACG TACGGAAGGA TCACCAAGGA CGTGGTTCTG
    CAGTGGGGAG AGATGCCGAC ATCTGTAGCA TATATCCGGT CGAATCAGAC AATGGGCTGG
    GGAGAGAAGG CAATAGAGAT ACGATCTGTG GAAACTGGTC ACTTGGATGG TGTATTTATG
    CACAAAAGGG CTCAGAGACT AAAGTTCCTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC
    TCCGTCCGCT CTGGTGGCAG CAGCCAGGTC TATTTCATGA CCTTAGGCAG GACTTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.