MAP4K4 cDNA ORF clone, Gallus gallus(chicken)

The following MAP4K4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP4K4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa143050 XM_015277799.2
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa143051 XM_015277800.2
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa143052 XM_015277801.2
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa143053 XM_015277802.2
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa143054 XM_015277803.2
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa143055 XM_015277805.2
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa143056 XM_015277806.2
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa143057 XM_015277807.2
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa143058 XM_015277808.2
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa143059 XM_015277809.2
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa30633 XM_015277799.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OGa30634 XM_015277800.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OGa30635 XM_015277801.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OGa30636 XM_015277802.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OGa30637 XM_015277803.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa30638 XM_015277805.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa30639 XM_015277806.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa30640 XM_015277807.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa30641 XM_015277808.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OGa30642 XM_015277809.1
Latest version!
Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa143050
    Clone ID Related Accession (Same CDS sequence) XM_015277799.2
    Accession Version XM_015277799.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4161bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277799.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GGAGAAGTGG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGAGTA CAGTTGACAA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCCTCTTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTAACTA CTAATGAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGTGGGACA
    ACAGTGACTT CTGTGGTAGG ATTTTCTAGT GAAGCAATGA GAACAGAGGC AATAAGGCAA
    GACCCTACAC GGAAAGGATC AGTTGTCAAC GTGAATCCTA CAAACACAAG ACCACAGAGC
    GATACCCCAG AGATCCGCAA ATACAAGAAA AGATTCAATT CTGAGATACT GTGTGCTGCC
    TTATGGGGAG TGAACTTGTT AGTGGGCACA GAAAGTGGTC TGATGCTGCT AGACAGAAGT
    GGTCAGGGGA AGGTTTATCC GCTCATCAAT CGAAGAAGAT TCCAGCAAAT GGATGTACTT
    GAAGGGCTAA ATGTCTTGGT GACAATTTCT GGTAAGAAGA ACAAGTTGCG AGTTTACTAC
    TTGTCCTGGT TGAGAAATAA AATCCTTCAC AATGATCCCG AAGTAGAAAA GAAACAGGGT
    TGGACAACGG TGGGTGACTT GGAGGGCTGT GTGCACTATA AAGTTGTAAA ATATGAGAGA
    ATCAAATTCT TGGTAATTGC GTTGAAGAGT TCTGTGGAAG TCTATGCATG GGCGCCAAAG
    CCATACCACA AATTTATGGC CTTTAAGTCA TTTGGTGAAT TGGTACATAA GCCATTGCTG
    GTGGATCTAA CTGTAGAAGA GGGTCAGAGA CTAAAAGTCA TTTATGGCTC CTGTGCTGGC
    TTCCATGCTG TTGACGTGGA TTCGGGATCA GTCTACGATA TTTATCTACC AACGCATATC
    CAGAGCAGTA TCCAACCTCA CGCAATCATA ATTCTCCCAA ATACGGATGG AATGGAGCTT
    CTGGTCTGTT ATGAGGATGA AGGAGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT
    GTGGTTCTGC AGTGGGGAGA AATGCCAACT TCAGTTGCAT ATATTCGCTC CAATCAGATT
    ATGGGCTGGG GAGAAAAAGC GATCGAAATA CGCTCGGTAG AAACTGGACA CTTGGACGGT
    GTGTTCATGC ACAAAAGGGC ACAAAGACTC AAGTTCTTAT GTGAACGCAA TGACAAGGTT
    TTCTTTGCCT CCGTACGGTC GGGTGGAAGC AGTCAAGTTT ATTTCATGAC CCTTGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133285.1
    CDS684..4844
    Translation

    Target ORF information:

    RefSeq Version XM_015277799.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277799.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GGAGAAGTGG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGAGTA CAGTTGACAA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCCTCTTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTAACTA CTAATGAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGTGGGACA
    ACAGTGACTT CTGTGGTAGG ATTTTCTAGT GAAGCAATGA GAACAGAGGC AATAAGGCAA
    GACCCTACAC GGAAAGGATC AGTTGTCAAC GTGAATCCTA CAAACACAAG ACCACAGAGC
    GATACCCCAG AGATCCGCAA ATACAAGAAA AGATTCAATT CTGAGATACT GTGTGCTGCC
    TTATGGGGAG TGAACTTGTT AGTGGGCACA GAAAGTGGTC TGATGCTGCT AGACAGAAGT
    GGTCAGGGGA AGGTTTATCC GCTCATCAAT CGAAGAAGAT TCCAGCAAAT GGATGTACTT
    GAAGGGCTAA ATGTCTTGGT GACAATTTCT GGTAAGAAGA ACAAGTTGCG AGTTTACTAC
    TTGTCCTGGT TGAGAAATAA AATCCTTCAC AATGATCCCG AAGTAGAAAA GAAACAGGGT
    TGGACAACGG TGGGTGACTT GGAGGGCTGT GTGCACTATA AAGTTGTAAA ATATGAGAGA
    ATCAAATTCT TGGTAATTGC GTTGAAGAGT TCTGTGGAAG TCTATGCATG GGCGCCAAAG
    CCATACCACA AATTTATGGC CTTTAAGTCA TTTGGTGAAT TGGTACATAA GCCATTGCTG
    GTGGATCTAA CTGTAGAAGA GGGTCAGAGA CTAAAAGTCA TTTATGGCTC CTGTGCTGGC
    TTCCATGCTG TTGACGTGGA TTCGGGATCA GTCTACGATA TTTATCTACC AACGCATATC
    CAGAGCAGTA TCCAACCTCA CGCAATCATA ATTCTCCCAA ATACGGATGG AATGGAGCTT
    CTGGTCTGTT ATGAGGATGA AGGAGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT
    GTGGTTCTGC AGTGGGGAGA AATGCCAACT TCAGTTGCAT ATATTCGCTC CAATCAGATT
    ATGGGCTGGG GAGAAAAAGC GATCGAAATA CGCTCGGTAG AAACTGGACA CTTGGACGGT
    GTGTTCATGC ACAAAAGGGC ACAAAGACTC AAGTTCTTAT GTGAACGCAA TGACAAGGTT
    TTCTTTGCCT CCGTACGGTC GGGTGGAAGC AGTCAAGTTT ATTTCATGAC CCTTGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143051
    Clone ID Related Accession (Same CDS sequence) XM_015277800.2
    Accession Version XM_015277800.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277800.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GGAGAAGTGG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGAGTA CAGTTGACAA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCCTCTTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTAACTA CTAATGAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGTGGGACA
    ACAGTGACTT CTGTGGGATT TTCTAGTGAA GCAATGAGAA CAGAGGCAAT AAGGCAAGAC
    CCTACACGGA AAGGATCAGT TGTCAACGTG AATCCTACAA ACACAAGACC ACAGAGCGAT
    ACCCCAGAGA TCCGCAAATA CAAGAAAAGA TTCAATTCTG AGATACTGTG TGCTGCCTTA
    TGGGGAGTGA ACTTGTTAGT GGGCACAGAA AGTGGTCTGA TGCTGCTAGA CAGAAGTGGT
    CAGGGGAAGG TTTATCCGCT CATCAATCGA AGAAGATTCC AGCAAATGGA TGTACTTGAA
    GGGCTAAATG TCTTGGTGAC AATTTCTGGT AAGAAGAACA AGTTGCGAGT TTACTACTTG
    TCCTGGTTGA GAAATAAAAT CCTTCACAAT GATCCCGAAG TAGAAAAGAA ACAGGGTTGG
    ACAACGGTGG GTGACTTGGA GGGCTGTGTG CACTATAAAG TTGTAAAATA TGAGAGAATC
    AAATTCTTGG TAATTGCGTT GAAGAGTTCT GTGGAAGTCT ATGCATGGGC GCCAAAGCCA
    TACCACAAAT TTATGGCCTT TAAGTCATTT GGTGAATTGG TACATAAGCC ATTGCTGGTG
    GATCTAACTG TAGAAGAGGG TCAGAGACTA AAAGTCATTT ATGGCTCCTG TGCTGGCTTC
    CATGCTGTTG ACGTGGATTC GGGATCAGTC TACGATATTT ATCTACCAAC GCATATCCAG
    AGCAGTATCC AACCTCACGC AATCATAATT CTCCCAAATA CGGATGGAAT GGAGCTTCTG
    GTCTGTTATG AGGATGAAGG AGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTG
    GTTCTGCAGT GGGGAGAAAT GCCAACTTCA GTTGCATATA TTCGCTCCAA TCAGATTATG
    GGCTGGGGAG AAAAAGCGAT CGAAATACGC TCGGTAGAAA CTGGACACTT GGACGGTGTG
    TTCATGCACA AAAGGGCACA AAGACTCAAG TTCTTATGTG AACGCAATGA CAAGGTTTTC
    TTTGCCTCCG TACGGTCGGG TGGAAGCAGT CAAGTTTATT TCATGACCCT TGGCAGGACT
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133286.1
    CDS686..4843
    Translation

    Target ORF information:

    RefSeq Version XM_015277800.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277800.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GGAGAAGTGG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGAGTA CAGTTGACAA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCCTCTTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTAACTA CTAATGAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGTGGGACA
    ACAGTGACTT CTGTGGGATT TTCTAGTGAA GCAATGAGAA CAGAGGCAAT AAGGCAAGAC
    CCTACACGGA AAGGATCAGT TGTCAACGTG AATCCTACAA ACACAAGACC ACAGAGCGAT
    ACCCCAGAGA TCCGCAAATA CAAGAAAAGA TTCAATTCTG AGATACTGTG TGCTGCCTTA
    TGGGGAGTGA ACTTGTTAGT GGGCACAGAA AGTGGTCTGA TGCTGCTAGA CAGAAGTGGT
    CAGGGGAAGG TTTATCCGCT CATCAATCGA AGAAGATTCC AGCAAATGGA TGTACTTGAA
    GGGCTAAATG TCTTGGTGAC AATTTCTGGT AAGAAGAACA AGTTGCGAGT TTACTACTTG
    TCCTGGTTGA GAAATAAAAT CCTTCACAAT GATCCCGAAG TAGAAAAGAA ACAGGGTTGG
    ACAACGGTGG GTGACTTGGA GGGCTGTGTG CACTATAAAG TTGTAAAATA TGAGAGAATC
    AAATTCTTGG TAATTGCGTT GAAGAGTTCT GTGGAAGTCT ATGCATGGGC GCCAAAGCCA
    TACCACAAAT TTATGGCCTT TAAGTCATTT GGTGAATTGG TACATAAGCC ATTGCTGGTG
    GATCTAACTG TAGAAGAGGG TCAGAGACTA AAAGTCATTT ATGGCTCCTG TGCTGGCTTC
    CATGCTGTTG ACGTGGATTC GGGATCAGTC TACGATATTT ATCTACCAAC GCATATCCAG
    AGCAGTATCC AACCTCACGC AATCATAATT CTCCCAAATA CGGATGGAAT GGAGCTTCTG
    GTCTGTTATG AGGATGAAGG AGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTG
    GTTCTGCAGT GGGGAGAAAT GCCAACTTCA GTTGCATATA TTCGCTCCAA TCAGATTATG
    GGCTGGGGAG AAAAAGCGAT CGAAATACGC TCGGTAGAAA CTGGACACTT GGACGGTGTG
    TTCATGCACA AAAGGGCACA AAGACTCAAG TTCTTATGTG AACGCAATGA CAAGGTTTTC
    TTTGCCTCCG TACGGTCGGG TGGAAGCAGT CAAGTTTATT TCATGACCCT TGGCAGGACT
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143052
    Clone ID Related Accession (Same CDS sequence) XM_015277801.2
    Accession Version XM_015277801.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277801.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GATTTAACTG CATTGGCAAA GGAGCTGCGA GCAGTAGAGG ATGTGCGACC TCCACATAAA
    GTGACGGATT ATTCGTCCTC AAGTGAGGAG TCAGGGACGA CAGATGAAGA AGATGATGAT
    ATGGAACAAG AAGGAGCAGA TGAATCTACT TCTGGACCAG ATGATGTCCG AGCAGTGTCA
    ACACTGAACT TGAGCAATGG TGAAACAGAG TCTGTAAAAA CTATGATTGT CCATGATGAT
    GTGGAGAGTG AACCTGCCTT GACTCCTTCT AAGGAGGGCA CCTTAATTGT CCGACAGAGT
    ACAGTTGACA AAAAGCGTGC CAGCCATCAT GAGAGCAATG GCTTTGCCGG TCGCATTCAC
    CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCCTCTTCC
    CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTAACT
    ACTAATGAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGGAC AACAGTGACT
    TCTGTGGTAG GATTTTCTAG TGAAGCAATG AGAACAGAGG CAATAAGGCA AGACCCTACA
    CGGAAAGGAT CAGTTGTCAA CGTGAATCCT ACAAACACAA GACCACAGAG CGATACCCCA
    GAGATCCGCA AATACAAGAA AAGATTCAAT TCTGAGATAC TGTGTGCTGC CTTATGGGGA
    GTGAACTTGT TAGTGGGCAC AGAAAGTGGT CTGATGCTGC TAGACAGAAG TGGTCAGGGG
    AAGGTTTATC CGCTCATCAA TCGAAGAAGA TTCCAGCAAA TGGATGTACT TGAAGGGCTA
    AATGTCTTGG TGACAATTTC TGGTAAGAAG AACAAGTTGC GAGTTTACTA CTTGTCCTGG
    TTGAGAAATA AAATCCTTCA CAATGATCCC GAAGTAGAAA AGAAACAGGG TTGGACAACG
    GTGGGTGACT TGGAGGGCTG TGTGCACTAT AAAGTTGTAA AATATGAGAG AATCAAATTC
    TTGGTAATTG CGTTGAAGAG TTCTGTGGAA GTCTATGCAT GGGCGCCAAA GCCATACCAC
    AAATTTATGG CCTTTAAGTC ATTTGGTGAA TTGGTACATA AGCCATTGCT GGTGGATCTA
    ACTGTAGAAG AGGGTCAGAG ACTAAAAGTC ATTTATGGCT CCTGTGCTGG CTTCCATGCT
    GTTGACGTGG ATTCGGGATC AGTCTACGAT ATTTATCTAC CAACGCATAT CCAGAGCAGT
    ATCCAACCTC ACGCAATCAT AATTCTCCCA AATACGGATG GAATGGAGCT TCTGGTCTGT
    TATGAGGATG AAGGAGTTTA TGTAAACACA TATGGAAGGA TCACCAAGGA TGTGGTTCTG
    CAGTGGGGAG AAATGCCAAC TTCAGTTGCA TATATTCGCT CCAATCAGAT TATGGGCTGG
    GGAGAAAAAG CGATCGAAAT ACGCTCGGTA GAAACTGGAC ACTTGGACGG TGTGTTCATG
    CACAAAAGGG CACAAAGACT CAAGTTCTTA TGTGAACGCA ATGACAAGGT TTTCTTTGCC
    TCCGTACGGT CGGGTGGAAG CAGTCAAGTT TATTTCATGA CCCTTGGCAG GACTTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133287.1
    CDS683..4834
    Translation

    Target ORF information:

    RefSeq Version XM_015277801.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277801.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GATTTAACTG CATTGGCAAA GGAGCTGCGA GCAGTAGAGG ATGTGCGACC TCCACATAAA
    GTGACGGATT ATTCGTCCTC AAGTGAGGAG TCAGGGACGA CAGATGAAGA AGATGATGAT
    ATGGAACAAG AAGGAGCAGA TGAATCTACT TCTGGACCAG ATGATGTCCG AGCAGTGTCA
    ACACTGAACT TGAGCAATGG TGAAACAGAG TCTGTAAAAA CTATGATTGT CCATGATGAT
    GTGGAGAGTG AACCTGCCTT GACTCCTTCT AAGGAGGGCA CCTTAATTGT CCGACAGAGT
    ACAGTTGACA AAAAGCGTGC CAGCCATCAT GAGAGCAATG GCTTTGCCGG TCGCATTCAC
    CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCCTCTTCC
    CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTAACT
    ACTAATGAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGGAC AACAGTGACT
    TCTGTGGTAG GATTTTCTAG TGAAGCAATG AGAACAGAGG CAATAAGGCA AGACCCTACA
    CGGAAAGGAT CAGTTGTCAA CGTGAATCCT ACAAACACAA GACCACAGAG CGATACCCCA
    GAGATCCGCA AATACAAGAA AAGATTCAAT TCTGAGATAC TGTGTGCTGC CTTATGGGGA
    GTGAACTTGT TAGTGGGCAC AGAAAGTGGT CTGATGCTGC TAGACAGAAG TGGTCAGGGG
    AAGGTTTATC CGCTCATCAA TCGAAGAAGA TTCCAGCAAA TGGATGTACT TGAAGGGCTA
    AATGTCTTGG TGACAATTTC TGGTAAGAAG AACAAGTTGC GAGTTTACTA CTTGTCCTGG
    TTGAGAAATA AAATCCTTCA CAATGATCCC GAAGTAGAAA AGAAACAGGG TTGGACAACG
    GTGGGTGACT TGGAGGGCTG TGTGCACTAT AAAGTTGTAA AATATGAGAG AATCAAATTC
    TTGGTAATTG CGTTGAAGAG TTCTGTGGAA GTCTATGCAT GGGCGCCAAA GCCATACCAC
    AAATTTATGG CCTTTAAGTC ATTTGGTGAA TTGGTACATA AGCCATTGCT GGTGGATCTA
    ACTGTAGAAG AGGGTCAGAG ACTAAAAGTC ATTTATGGCT CCTGTGCTGG CTTCCATGCT
    GTTGACGTGG ATTCGGGATC AGTCTACGAT ATTTATCTAC CAACGCATAT CCAGAGCAGT
    ATCCAACCTC ACGCAATCAT AATTCTCCCA AATACGGATG GAATGGAGCT TCTGGTCTGT
    TATGAGGATG AAGGAGTTTA TGTAAACACA TATGGAAGGA TCACCAAGGA TGTGGTTCTG
    CAGTGGGGAG AAATGCCAAC TTCAGTTGCA TATATTCGCT CCAATCAGAT TATGGGCTGG
    GGAGAAAAAG CGATCGAAAT ACGCTCGGTA GAAACTGGAC ACTTGGACGG TGTGTTCATG
    CACAAAAGGG CACAAAGACT CAAGTTCTTA TGTGAACGCA ATGACAAGGT TTTCTTTGCC
    TCCGTACGGT CGGGTGGAAG CAGTCAAGTT TATTTCATGA CCCTTGGCAG GACTTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143053
    Clone ID Related Accession (Same CDS sequence) XM_015277802.2
    Accession Version XM_015277802.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAATA CAGATGGCGA GAGATGGAGG AGCATCGACA GGCAGAGAGG
    CTCCAGCGCC AGTTGCAGCA GGAGCAAGCC TACCTCCTGT CACTGCAGCA TGATCACAGG
    CGGCAGCAGC AGCAGCAACA GCAGCAACAG CAGCAACAGC AGCAGCAGCA AGAAAGAAAT
    AAACAAAGCT ATCATACCCC TGAGCCTAAA TCCCACTATG AGCCTGCTGA AAGAGCGCGT
    GAGGTGGAGG AGAGATTTAG AAAAACGAAT CAGGGCTCCC CTGAAGCACA GTCTAAGCAG
    ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT TCAAGATCAG AGTCCTTTTC CAATGGAAAC
    TCAGAACCTG CTCAGCCTGC CCTGCAGAGG CCAATGGAGC CCCAAGTACA GTGGTCCCAT
    CTGGCATCTC TCAAGAACAA TGTTTCCCCT GTCTCGCGAT CCCATTCCTT CAGTGACCCT
    TCTCCCAAAT TTGCTCATCA CCATCTTCGT TCCCAGGACC CATATCCACC TTCACGCAGT
    GAAGTGCTAA ATCAGAGTTC TGACTCTAAG TCGGAGGTAC CCGACCCCAC CCAAAAGGCT
    TGGGCTAGAT CAGACAGTGA CGAGGTGCCT CCAAGGGTAC CTGTGAGAAC AACATCCCGA
    TCACCAGTCC TGTCCCGTCG TGATTCTCCA CTGCAGGGCA GTGGGCAGCA AAATAACCAA
    GCAGGTCAAA GAAACTCCAC GAGCAATATA GAGCCTCGTC TGCTGTGGGA AAGGGTGGAG
    AAGCTTGTAC CAAGACCAGG CAGTGGCAGT TCTTCAGGTT CAAGCAACTC CGGCTCACAG
    CCTGGTTCCC ACCCTGGCTC TCAGAGTGGG TCTGGAGAGC GGTTCCGGAT GAGATCATCA
    TCCAAATCAG AGGGTTCACC ATCGCAGCGT CATGAAAGTG CACCTAAAAA GCCTGAAGAG
    AAAAAGGAGG TCTTCAGACC TATCAAGCCT GCTGGAGAAG TGGATTTAAC TGCATTGGCA
    AAGGAGCTGC GAGCAGTAGA GGATGTGCGA CCTCCACATA AAGTGACGGA TTATTCGTCC
    TCAAGTGAGG AGTCAGGGAC GACAGATGAA GAAGATGATG ATATGGAACA AGAAGGAGCA
    GATGAATCTA CTTCTGGACC AGATGATGTC CGAGCAGTGT CAACACTGAA CTTGAGCAAT
    GGTGAAACAG AGTCTGTAAA AACTATGATT GTCCATGATG ATGTGGAGAG TGAACCTGCC
    TTGACTCCTT CTAAGGAGGG CACCTTAATT GTCCGACAGA GTACAGTTGA CAAAAAGCGT
    GCCAGCCATC ATGAGAGCAA TGGCTTTGCC GGTCGCATTC ACCTCTTGCC AGATCTCTTA
    CAGCAAAGCC ATTCCTCCTC CACTTCCTCC ACCTCCTCTT CCCCATCCTC CAGCCAGCCG
    ACACCCACCA TGTCCCCACA GACACCCCAG GACAAGCTAA CTACTAATGA GACTCAGTCC
    GCTAGTAACA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC
    AGATTACTAC AGATTTCTCC ATCTAGTGGG ACAACAGTGA CTTCTGTGGT AGGATTTTCT
    AGTGAAGCAA TGAGAACAGA GGCAATAAGG CAAGACCCTA CACGGAAAGG ATCAGTTGTC
    AACGTGAATC CTACAAACAC AAGACCACAG AGCGATACCC CAGAGATCCG CAAATACAAG
    AAAAGATTCA ATTCTGAGAT ACTGTGTGCT GCCTTATGGG GAGTGAACTT GTTAGTGGGC
    ACAGAAAGTG GTCTGATGCT GCTAGACAGA AGTGGTCAGG GGAAGGTTTA TCCGCTCATC
    AATCGAAGAA GATTCCAGCA AATGGATGTA CTTGAAGGGC TAAATGTCTT GGTGACAATT
    TCTGGTAAGA AGAACAAGTT GCGAGTTTAC TACTTGTCCT GGTTGAGAAA TAAAATCCTT
    CACAATGATC CCGAAGTAGA AAAGAAACAG GGTTGGACAA CGGTGGGTGA CTTGGAGGGC
    TGTGTGCACT ATAAAGTTGT AAAATATGAG AGAATCAAAT TCTTGGTAAT TGCGTTGAAG
    AGTTCTGTGG AAGTCTATGC ATGGGCGCCA AAGCCATACC ACAAATTTAT GGCCTTTAAG
    TCATTTGGTG AATTGGTACA TAAGCCATTG CTGGTGGATC TAACTGTAGA AGAGGGTCAG
    AGACTAAAAG TCATTTATGG CTCCTGTGCT GGCTTCCATG CTGTTGACGT GGATTCGGGA
    TCAGTCTACG ATATTTATCT ACCAACGCAT ATCCAGAGCA GTATCCAACC TCACGCAATC
    ATAATTCTCC CAAATACGGA TGGAATGGAG CTTCTGGTCT GTTATGAGGA TGAAGGAGTT
    TATGTAAACA CATATGGAAG GATCACCAAG GATGTGGTTC TGCAGTGGGG AGAAATGCCA
    ACTTCAGTTG CATATATTCG CTCCAATCAG ATTATGGGCT GGGGAGAAAA AGCGATCGAA
    ATACGCTCGG TAGAAACTGG ACACTTGGAC GGTGTGTTCA TGCACAAAAG GGCACAAAGA
    CTCAAGTTCT TATGTGAACG CAATGACAAG GTTTTCTTTG CCTCCGTACG GTCGGGTGGA
    AGCAGTCAAG TTTATTTCAT GACCCTTGGC AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133288.1
    CDS684..4757
    Translation

    Target ORF information:

    RefSeq Version XM_015277802.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277802.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAATA CAGATGGCGA GAGATGGAGG AGCATCGACA GGCAGAGAGG
    CTCCAGCGCC AGTTGCAGCA GGAGCAAGCC TACCTCCTGT CACTGCAGCA TGATCACAGG
    CGGCAGCAGC AGCAGCAACA GCAGCAACAG CAGCAACAGC AGCAGCAGCA AGAAAGAAAT
    AAACAAAGCT ATCATACCCC TGAGCCTAAA TCCCACTATG AGCCTGCTGA AAGAGCGCGT
    GAGGTGGAGG AGAGATTTAG AAAAACGAAT CAGGGCTCCC CTGAAGCACA GTCTAAGCAG
    ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT TCAAGATCAG AGTCCTTTTC CAATGGAAAC
    TCAGAACCTG CTCAGCCTGC CCTGCAGAGG CCAATGGAGC CCCAAGTACA GTGGTCCCAT
    CTGGCATCTC TCAAGAACAA TGTTTCCCCT GTCTCGCGAT CCCATTCCTT CAGTGACCCT
    TCTCCCAAAT TTGCTCATCA CCATCTTCGT TCCCAGGACC CATATCCACC TTCACGCAGT
    GAAGTGCTAA ATCAGAGTTC TGACTCTAAG TCGGAGGTAC CCGACCCCAC CCAAAAGGCT
    TGGGCTAGAT CAGACAGTGA CGAGGTGCCT CCAAGGGTAC CTGTGAGAAC AACATCCCGA
    TCACCAGTCC TGTCCCGTCG TGATTCTCCA CTGCAGGGCA GTGGGCAGCA AAATAACCAA
    GCAGGTCAAA GAAACTCCAC GAGCAATATA GAGCCTCGTC TGCTGTGGGA AAGGGTGGAG
    AAGCTTGTAC CAAGACCAGG CAGTGGCAGT TCTTCAGGTT CAAGCAACTC CGGCTCACAG
    CCTGGTTCCC ACCCTGGCTC TCAGAGTGGG TCTGGAGAGC GGTTCCGGAT GAGATCATCA
    TCCAAATCAG AGGGTTCACC ATCGCAGCGT CATGAAAGTG CACCTAAAAA GCCTGAAGAG
    AAAAAGGAGG TCTTCAGACC TATCAAGCCT GCTGGAGAAG TGGATTTAAC TGCATTGGCA
    AAGGAGCTGC GAGCAGTAGA GGATGTGCGA CCTCCACATA AAGTGACGGA TTATTCGTCC
    TCAAGTGAGG AGTCAGGGAC GACAGATGAA GAAGATGATG ATATGGAACA AGAAGGAGCA
    GATGAATCTA CTTCTGGACC AGATGATGTC CGAGCAGTGT CAACACTGAA CTTGAGCAAT
    GGTGAAACAG AGTCTGTAAA AACTATGATT GTCCATGATG ATGTGGAGAG TGAACCTGCC
    TTGACTCCTT CTAAGGAGGG CACCTTAATT GTCCGACAGA GTACAGTTGA CAAAAAGCGT
    GCCAGCCATC ATGAGAGCAA TGGCTTTGCC GGTCGCATTC ACCTCTTGCC AGATCTCTTA
    CAGCAAAGCC ATTCCTCCTC CACTTCCTCC ACCTCCTCTT CCCCATCCTC CAGCCAGCCG
    ACACCCACCA TGTCCCCACA GACACCCCAG GACAAGCTAA CTACTAATGA GACTCAGTCC
    GCTAGTAACA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC
    AGATTACTAC AGATTTCTCC ATCTAGTGGG ACAACAGTGA CTTCTGTGGT AGGATTTTCT
    AGTGAAGCAA TGAGAACAGA GGCAATAAGG CAAGACCCTA CACGGAAAGG ATCAGTTGTC
    AACGTGAATC CTACAAACAC AAGACCACAG AGCGATACCC CAGAGATCCG CAAATACAAG
    AAAAGATTCA ATTCTGAGAT ACTGTGTGCT GCCTTATGGG GAGTGAACTT GTTAGTGGGC
    ACAGAAAGTG GTCTGATGCT GCTAGACAGA AGTGGTCAGG GGAAGGTTTA TCCGCTCATC
    AATCGAAGAA GATTCCAGCA AATGGATGTA CTTGAAGGGC TAAATGTCTT GGTGACAATT
    TCTGGTAAGA AGAACAAGTT GCGAGTTTAC TACTTGTCCT GGTTGAGAAA TAAAATCCTT
    CACAATGATC CCGAAGTAGA AAAGAAACAG GGTTGGACAA CGGTGGGTGA CTTGGAGGGC
    TGTGTGCACT ATAAAGTTGT AAAATATGAG AGAATCAAAT TCTTGGTAAT TGCGTTGAAG
    AGTTCTGTGG AAGTCTATGC ATGGGCGCCA AAGCCATACC ACAAATTTAT GGCCTTTAAG
    TCATTTGGTG AATTGGTACA TAAGCCATTG CTGGTGGATC TAACTGTAGA AGAGGGTCAG
    AGACTAAAAG TCATTTATGG CTCCTGTGCT GGCTTCCATG CTGTTGACGT GGATTCGGGA
    TCAGTCTACG ATATTTATCT ACCAACGCAT ATCCAGAGCA GTATCCAACC TCACGCAATC
    ATAATTCTCC CAAATACGGA TGGAATGGAG CTTCTGGTCT GTTATGAGGA TGAAGGAGTT
    TATGTAAACA CATATGGAAG GATCACCAAG GATGTGGTTC TGCAGTGGGG AGAAATGCCA
    ACTTCAGTTG CATATATTCG CTCCAATCAG ATTATGGGCT GGGGAGAAAA AGCGATCGAA
    ATACGCTCGG TAGAAACTGG ACACTTGGAC GGTGTGTTCA TGCACAAAAG GGCACAAAGA
    CTCAAGTTCT TATGTGAACG CAATGACAAG GTTTTCTTTG CCTCCGTACG GTCGGGTGGA
    AGCAGTCAAG TTTATTTCAT GACCCTTGGC AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143054
    Clone ID Related Accession (Same CDS sequence) XM_015277803.2
    Accession Version XM_015277803.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277803.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GGAGAAGTGG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGGACAAC AGTGACTTCT
    GTGGTAGGAT TTTCTAGTGA AGCAATGAGA ACAGAGGCAA TAAGGCAAGA CCCTACACGG
    AAAGGATCAG TTGTCAACGT GAATCCTACA AACACAAGAC CACAGAGCGA TACCCCAGAG
    ATCCGCAAAT ACAAGAAAAG ATTCAATTCT GAGATACTGT GTGCTGCCTT ATGGGGAGTG
    AACTTGTTAG TGGGCACAGA AAGTGGTCTG ATGCTGCTAG ACAGAAGTGG TCAGGGGAAG
    GTTTATCCGC TCATCAATCG AAGAAGATTC CAGCAAATGG ATGTACTTGA AGGGCTAAAT
    GTCTTGGTGA CAATTTCTGG TAAGAAGAAC AAGTTGCGAG TTTACTACTT GTCCTGGTTG
    AGAAATAAAA TCCTTCACAA TGATCCCGAA GTAGAAAAGA AACAGGGTTG GACAACGGTG
    GGTGACTTGG AGGGCTGTGT GCACTATAAA GTTGTAAAAT ATGAGAGAAT CAAATTCTTG
    GTAATTGCGT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CGCCAAAGCC ATACCACAAA
    TTTATGGCCT TTAAGTCATT TGGTGAATTG GTACATAAGC CATTGCTGGT GGATCTAACT
    GTAGAAGAGG GTCAGAGACT AAAAGTCATT TATGGCTCCT GTGCTGGCTT CCATGCTGTT
    GACGTGGATT CGGGATCAGT CTACGATATT TATCTACCAA CGCATATCCA GAGCAGTATC
    CAACCTCACG CAATCATAAT TCTCCCAAAT ACGGATGGAA TGGAGCTTCT GGTCTGTTAT
    GAGGATGAAG GAGTTTATGT AAACACATAT GGAAGGATCA CCAAGGATGT GGTTCTGCAG
    TGGGGAGAAA TGCCAACTTC AGTTGCATAT ATTCGCTCCA ATCAGATTAT GGGCTGGGGA
    GAAAAAGCGA TCGAAATACG CTCGGTAGAA ACTGGACACT TGGACGGTGT GTTCATGCAC
    AAAAGGGCAC AAAGACTCAA GTTCTTATGT GAACGCAATG ACAAGGTTTT CTTTGCCTCC
    GTACGGTCGG GTGGAAGCAG TCAAGTTTAT TTCATGACCC TTGGCAGGAC TTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133289.1
    CDS685..4653
    Translation

    Target ORF information:

    RefSeq Version XM_015277803.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277803.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GGAGAAGTGG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGGACAAC AGTGACTTCT
    GTGGTAGGAT TTTCTAGTGA AGCAATGAGA ACAGAGGCAA TAAGGCAAGA CCCTACACGG
    AAAGGATCAG TTGTCAACGT GAATCCTACA AACACAAGAC CACAGAGCGA TACCCCAGAG
    ATCCGCAAAT ACAAGAAAAG ATTCAATTCT GAGATACTGT GTGCTGCCTT ATGGGGAGTG
    AACTTGTTAG TGGGCACAGA AAGTGGTCTG ATGCTGCTAG ACAGAAGTGG TCAGGGGAAG
    GTTTATCCGC TCATCAATCG AAGAAGATTC CAGCAAATGG ATGTACTTGA AGGGCTAAAT
    GTCTTGGTGA CAATTTCTGG TAAGAAGAAC AAGTTGCGAG TTTACTACTT GTCCTGGTTG
    AGAAATAAAA TCCTTCACAA TGATCCCGAA GTAGAAAAGA AACAGGGTTG GACAACGGTG
    GGTGACTTGG AGGGCTGTGT GCACTATAAA GTTGTAAAAT ATGAGAGAAT CAAATTCTTG
    GTAATTGCGT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CGCCAAAGCC ATACCACAAA
    TTTATGGCCT TTAAGTCATT TGGTGAATTG GTACATAAGC CATTGCTGGT GGATCTAACT
    GTAGAAGAGG GTCAGAGACT AAAAGTCATT TATGGCTCCT GTGCTGGCTT CCATGCTGTT
    GACGTGGATT CGGGATCAGT CTACGATATT TATCTACCAA CGCATATCCA GAGCAGTATC
    CAACCTCACG CAATCATAAT TCTCCCAAAT ACGGATGGAA TGGAGCTTCT GGTCTGTTAT
    GAGGATGAAG GAGTTTATGT AAACACATAT GGAAGGATCA CCAAGGATGT GGTTCTGCAG
    TGGGGAGAAA TGCCAACTTC AGTTGCATAT ATTCGCTCCA ATCAGATTAT GGGCTGGGGA
    GAAAAAGCGA TCGAAATACG CTCGGTAGAA ACTGGACACT TGGACGGTGT GTTCATGCAC
    AAAAGGGCAC AAAGACTCAA GTTCTTATGT GAACGCAATG ACAAGGTTTT CTTTGCCTCC
    GTACGGTCGG GTGGAAGCAG TCAAGTTTAT TTCATGACCC TTGGCAGGAC TTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143055
    Clone ID Related Accession (Same CDS sequence) XM_015277805.2
    Accession Version XM_015277805.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277805.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GGAGAAGTGG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGGACAAC AGTGACTTCT
    GTGGGATTTT CTAGTGAAGC AATGAGAACA GAGGCAATAA GGCAAGACCC TACACGGAAA
    GGATCAGTTG TCAACGTGAA TCCTACAAAC ACAAGACCAC AGAGCGATAC CCCAGAGATC
    CGCAAATACA AGAAAAGATT CAATTCTGAG ATACTGTGTG CTGCCTTATG GGGAGTGAAC
    TTGTTAGTGG GCACAGAAAG TGGTCTGATG CTGCTAGACA GAAGTGGTCA GGGGAAGGTT
    TATCCGCTCA TCAATCGAAG AAGATTCCAG CAAATGGATG TACTTGAAGG GCTAAATGTC
    TTGGTGACAA TTTCTGGTAA GAAGAACAAG TTGCGAGTTT ACTACTTGTC CTGGTTGAGA
    AATAAAATCC TTCACAATGA TCCCGAAGTA GAAAAGAAAC AGGGTTGGAC AACGGTGGGT
    GACTTGGAGG GCTGTGTGCA CTATAAAGTT GTAAAATATG AGAGAATCAA ATTCTTGGTA
    ATTGCGTTGA AGAGTTCTGT GGAAGTCTAT GCATGGGCGC CAAAGCCATA CCACAAATTT
    ATGGCCTTTA AGTCATTTGG TGAATTGGTA CATAAGCCAT TGCTGGTGGA TCTAACTGTA
    GAAGAGGGTC AGAGACTAAA AGTCATTTAT GGCTCCTGTG CTGGCTTCCA TGCTGTTGAC
    GTGGATTCGG GATCAGTCTA CGATATTTAT CTACCAACGC ATATCCAGAG CAGTATCCAA
    CCTCACGCAA TCATAATTCT CCCAAATACG GATGGAATGG AGCTTCTGGT CTGTTATGAG
    GATGAAGGAG TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTGGT TCTGCAGTGG
    GGAGAAATGC CAACTTCAGT TGCATATATT CGCTCCAATC AGATTATGGG CTGGGGAGAA
    AAAGCGATCG AAATACGCTC GGTAGAAACT GGACACTTGG ACGGTGTGTT CATGCACAAA
    AGGGCACAAA GACTCAAGTT CTTATGTGAA CGCAATGACA AGGTTTTCTT TGCCTCCGTA
    CGGTCGGGTG GAAGCAGTCA AGTTTATTTC ATGACCCTTG GCAGGACTTC TCTTCTGAGC
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133291.1
    CDS687..4652
    Translation

    Target ORF information:

    RefSeq Version XM_015277805.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277805.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GGAGAAGTGG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGGACAAC AGTGACTTCT
    GTGGGATTTT CTAGTGAAGC AATGAGAACA GAGGCAATAA GGCAAGACCC TACACGGAAA
    GGATCAGTTG TCAACGTGAA TCCTACAAAC ACAAGACCAC AGAGCGATAC CCCAGAGATC
    CGCAAATACA AGAAAAGATT CAATTCTGAG ATACTGTGTG CTGCCTTATG GGGAGTGAAC
    TTGTTAGTGG GCACAGAAAG TGGTCTGATG CTGCTAGACA GAAGTGGTCA GGGGAAGGTT
    TATCCGCTCA TCAATCGAAG AAGATTCCAG CAAATGGATG TACTTGAAGG GCTAAATGTC
    TTGGTGACAA TTTCTGGTAA GAAGAACAAG TTGCGAGTTT ACTACTTGTC CTGGTTGAGA
    AATAAAATCC TTCACAATGA TCCCGAAGTA GAAAAGAAAC AGGGTTGGAC AACGGTGGGT
    GACTTGGAGG GCTGTGTGCA CTATAAAGTT GTAAAATATG AGAGAATCAA ATTCTTGGTA
    ATTGCGTTGA AGAGTTCTGT GGAAGTCTAT GCATGGGCGC CAAAGCCATA CCACAAATTT
    ATGGCCTTTA AGTCATTTGG TGAATTGGTA CATAAGCCAT TGCTGGTGGA TCTAACTGTA
    GAAGAGGGTC AGAGACTAAA AGTCATTTAT GGCTCCTGTG CTGGCTTCCA TGCTGTTGAC
    GTGGATTCGG GATCAGTCTA CGATATTTAT CTACCAACGC ATATCCAGAG CAGTATCCAA
    CCTCACGCAA TCATAATTCT CCCAAATACG GATGGAATGG AGCTTCTGGT CTGTTATGAG
    GATGAAGGAG TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTGGT TCTGCAGTGG
    GGAGAAATGC CAACTTCAGT TGCATATATT CGCTCCAATC AGATTATGGG CTGGGGAGAA
    AAAGCGATCG AAATACGCTC GGTAGAAACT GGACACTTGG ACGGTGTGTT CATGCACAAA
    AGGGCACAAA GACTCAAGTT CTTATGTGAA CGCAATGACA AGGTTTTCTT TGCCTCCGTA
    CGGTCGGGTG GAAGCAGTCA AGTTTATTTC ATGACCCTTG GCAGGACTTC TCTTCTGAGC
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143056
    Clone ID Related Accession (Same CDS sequence) XM_015277806.2
    Accession Version XM_015277806.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277806.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACCTGT GAGAACAACA TCCCGATCAC CAGTCCTGTC CCGTCGTGAT
    TCTCCACTGC AGGGCAGTGG GCAGCAAAAT AACCAAGCAG GTCAAAGAAA CTCCACGAGC
    AATATAGAGC CTCGTCTGCT GTGGGAAAGG GTGGAGAAGC TTGTACCAAG ACCAGGCAGT
    GGCAGTTCTT CAGGTTCAAG CAACTCCGGC TCACAGCCTG GTTCCCACCC TGGCTCTCAG
    AGTGGGTCTG GAGAGCGGTT CCGGATGAGA TCATCATCCA AATCAGAGGG TTCACCATCG
    CAGCGTCATG AAAGTGCACC TAAAAAGCCT GAAGAGAAAA AGGAGGTCTT CAGACCTATC
    AAGCCTGCTG GAGAAGTGGA TTTAACTGCA TTGGCAAAGG AGCTGCGAGC AGTAGAGGAT
    GTGCGACCTC CACATAAAGT GACGGATTAT TCGTCCTCAA GTGAGGAGTC AGGGACGACA
    GATGAAGAAG ATGATGATAT GGAACAAGAA GGAGCAGATG AATCTACTTC TGGACCAGAT
    GATGTCCGAG CAGTGTCAAC ACTGAACTTG AGCAATGGTG AAACAGAGTC TGTAAAAACT
    ATGATTGTCC ATGATGATGT GGAGAGTGAA CCTGCCTTGA CTCCTTCTAA GGAGGGCACC
    TTAATTGTCC GACAGAGTAC AGTTGACAAA AAGCGTGCCA GCCATCATGA GAGCAATGGC
    TTTGCCGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CCTCTTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTAACTAC TAATGAGACT CAGTCCGCTA GTAACACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT
    AGTGGGACAA CAGTGACTTC TGTGGTAGGA TTTTCTAGTG AAGCAATGAG AACAGAGGCA
    ATAAGGCAAG ACCCTACACG GAAAGGATCA GTTGTCAACG TGAATCCTAC AAACACAAGA
    CCACAGAGCG ATACCCCAGA GATCCGCAAA TACAAGAAAA GATTCAATTC TGAGATACTG
    TGTGCTGCCT TATGGGGAGT GAACTTGTTA GTGGGCACAG AAAGTGGTCT GATGCTGCTA
    GACAGAAGTG GTCAGGGGAA GGTTTATCCG CTCATCAATC GAAGAAGATT CCAGCAAATG
    GATGTACTTG AAGGGCTAAA TGTCTTGGTG ACAATTTCTG GTAAGAAGAA CAAGTTGCGA
    GTTTACTACT TGTCCTGGTT GAGAAATAAA ATCCTTCACA ATGATCCCGA AGTAGAAAAG
    AAACAGGGTT GGACAACGGT GGGTGACTTG GAGGGCTGTG TGCACTATAA AGTTGTAAAA
    TATGAGAGAA TCAAATTCTT GGTAATTGCG TTGAAGAGTT CTGTGGAAGT CTATGCATGG
    GCGCCAAAGC CATACCACAA ATTTATGGCC TTTAAGTCAT TTGGTGAATT GGTACATAAG
    CCATTGCTGG TGGATCTAAC TGTAGAAGAG GGTCAGAGAC TAAAAGTCAT TTATGGCTCC
    TGTGCTGGCT TCCATGCTGT TGACGTGGAT TCGGGATCAG TCTACGATAT TTATCTACCA
    ACGCATATCC AGAGCAGTAT CCAACCTCAC GCAATCATAA TTCTCCCAAA TACGGATGGA
    ATGGAGCTTC TGGTCTGTTA TGAGGATGAA GGAGTTTATG TAAACACATA TGGAAGGATC
    ACCAAGGATG TGGTTCTGCA GTGGGGAGAA ATGCCAACTT CAGTTGCATA TATTCGCTCC
    AATCAGATTA TGGGCTGGGG AGAAAAAGCG ATCGAAATAC GCTCGGTAGA AACTGGACAC
    TTGGACGGTG TGTTCATGCA CAAAAGGGCA CAAAGACTCA AGTTCTTATG TGAACGCAAT
    GACAAGGTTT TCTTTGCCTC CGTACGGTCG GGTGGAAGCA GTCAAGTTTA TTTCATGACC
    CTTGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133292.1
    CDS681..4610
    Translation

    Target ORF information:

    RefSeq Version XM_015277806.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277806.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACCTGT GAGAACAACA TCCCGATCAC CAGTCCTGTC CCGTCGTGAT
    TCTCCACTGC AGGGCAGTGG GCAGCAAAAT AACCAAGCAG GTCAAAGAAA CTCCACGAGC
    AATATAGAGC CTCGTCTGCT GTGGGAAAGG GTGGAGAAGC TTGTACCAAG ACCAGGCAGT
    GGCAGTTCTT CAGGTTCAAG CAACTCCGGC TCACAGCCTG GTTCCCACCC TGGCTCTCAG
    AGTGGGTCTG GAGAGCGGTT CCGGATGAGA TCATCATCCA AATCAGAGGG TTCACCATCG
    CAGCGTCATG AAAGTGCACC TAAAAAGCCT GAAGAGAAAA AGGAGGTCTT CAGACCTATC
    AAGCCTGCTG GAGAAGTGGA TTTAACTGCA TTGGCAAAGG AGCTGCGAGC AGTAGAGGAT
    GTGCGACCTC CACATAAAGT GACGGATTAT TCGTCCTCAA GTGAGGAGTC AGGGACGACA
    GATGAAGAAG ATGATGATAT GGAACAAGAA GGAGCAGATG AATCTACTTC TGGACCAGAT
    GATGTCCGAG CAGTGTCAAC ACTGAACTTG AGCAATGGTG AAACAGAGTC TGTAAAAACT
    ATGATTGTCC ATGATGATGT GGAGAGTGAA CCTGCCTTGA CTCCTTCTAA GGAGGGCACC
    TTAATTGTCC GACAGAGTAC AGTTGACAAA AAGCGTGCCA GCCATCATGA GAGCAATGGC
    TTTGCCGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CCTCTTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTAACTAC TAATGAGACT CAGTCCGCTA GTAACACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT
    AGTGGGACAA CAGTGACTTC TGTGGTAGGA TTTTCTAGTG AAGCAATGAG AACAGAGGCA
    ATAAGGCAAG ACCCTACACG GAAAGGATCA GTTGTCAACG TGAATCCTAC AAACACAAGA
    CCACAGAGCG ATACCCCAGA GATCCGCAAA TACAAGAAAA GATTCAATTC TGAGATACTG
    TGTGCTGCCT TATGGGGAGT GAACTTGTTA GTGGGCACAG AAAGTGGTCT GATGCTGCTA
    GACAGAAGTG GTCAGGGGAA GGTTTATCCG CTCATCAATC GAAGAAGATT CCAGCAAATG
    GATGTACTTG AAGGGCTAAA TGTCTTGGTG ACAATTTCTG GTAAGAAGAA CAAGTTGCGA
    GTTTACTACT TGTCCTGGTT GAGAAATAAA ATCCTTCACA ATGATCCCGA AGTAGAAAAG
    AAACAGGGTT GGACAACGGT GGGTGACTTG GAGGGCTGTG TGCACTATAA AGTTGTAAAA
    TATGAGAGAA TCAAATTCTT GGTAATTGCG TTGAAGAGTT CTGTGGAAGT CTATGCATGG
    GCGCCAAAGC CATACCACAA ATTTATGGCC TTTAAGTCAT TTGGTGAATT GGTACATAAG
    CCATTGCTGG TGGATCTAAC TGTAGAAGAG GGTCAGAGAC TAAAAGTCAT TTATGGCTCC
    TGTGCTGGCT TCCATGCTGT TGACGTGGAT TCGGGATCAG TCTACGATAT TTATCTACCA
    ACGCATATCC AGAGCAGTAT CCAACCTCAC GCAATCATAA TTCTCCCAAA TACGGATGGA
    ATGGAGCTTC TGGTCTGTTA TGAGGATGAA GGAGTTTATG TAAACACATA TGGAAGGATC
    ACCAAGGATG TGGTTCTGCA GTGGGGAGAA ATGCCAACTT CAGTTGCATA TATTCGCTCC
    AATCAGATTA TGGGCTGGGG AGAAAAAGCG ATCGAAATAC GCTCGGTAGA AACTGGACAC
    TTGGACGGTG TGTTCATGCA CAAAAGGGCA CAAAGACTCA AGTTCTTATG TGAACGCAAT
    GACAAGGTTT TCTTTGCCTC CGTACGGTCG GGTGGAAGCA GTCAAGTTTA TTTCATGACC
    CTTGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143057
    Clone ID Related Accession (Same CDS sequence) XM_015277807.2
    Accession Version XM_015277807.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277807.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACCTGT GAGAACAACA TCCCGATCAC CAGTCCTGTC CCGTCGTGAT
    TCTCCACTGC AGGGCAGTGG GCAGCAAAAT AACCAAGCAG GTCAAAGAAA CTCCACGAGC
    AATATAGAGC CTCGTCTGCT GTGGGAAAGG GTGGAGAAGC TTGTACCAAG ACCAGGCAGT
    GGCAGTTCTT CAGGTTCAAG CAACTCCGGC TCACAGCCTG GTTCCCACCC TGGCTCTCAG
    AGTGGGTCTG GAGAGCGGTT CCGGATGAGA TCATCATCCA AATCAGAGGG TTCACCATCG
    CAGCGTCATG AAAGTGCACC TAAAAAGCCT GAAGAGAAAA AGGAGGTCTT CAGACCTATC
    AAGCCTGCTG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGAGTA CAGTTGACAA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCCTCTTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTAACTA CTAATGAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGTGGGACA
    ACAGTGACTT CTGTGGTAGG ATTTTCTAGT GAAGCAATGA GAACAGAGGC AATAAGGCAA
    GACCCTACAC GGAAAGGATC AGTTGTCAAC GTGAATCCTA CAAACACAAG ACCACAGAGC
    GATACCCCAG AGATCCGCAA ATACAAGAAA AGATTCAATT CTGAGATACT GTGTGCTGCC
    TTATGGGGAG TGAACTTGTT AGTGGGCACA GAAAGTGGTC TGATGCTGCT AGACAGAAGT
    GGTCAGGGGA AGGTTTATCC GCTCATCAAT CGAAGAAGAT TCCAGCAAAT GGATGTACTT
    GAAGGGCTAA ATGTCTTGGT GACAATTTCT GGTAAGAAGA ACAAGTTGCG AGTTTACTAC
    TTGTCCTGGT TGAGAAATAA AATCCTTCAC AATGATCCCG AAGTAGAAAA GAAACAGGGT
    TGGACAACGG TGGGTGACTT GGAGGGCTGT GTGCACTATA AAGTTGTAAA ATATGAGAGA
    ATCAAATTCT TGGTAATTGC GTTGAAGAGT TCTGTGGAAG TCTATGCATG GGCGCCAAAG
    CCATACCACA AATTTATGGC CTTTAAGTCA TTTGGTGAAT TGGTACATAA GCCATTGCTG
    GTGGATCTAA CTGTAGAAGA GGGTCAGAGA CTAAAAGTCA TTTATGGCTC CTGTGCTGGC
    TTCCATGCTG TTGACGTGGA TTCGGGATCA GTCTACGATA TTTATCTACC AACGCATATC
    CAGAGCAGTA TCCAACCTCA CGCAATCATA ATTCTCCCAA ATACGGATGG AATGGAGCTT
    CTGGTCTGTT ATGAGGATGA AGGAGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT
    GTGGTTCTGC AGTGGGGAGA AATGCCAACT TCAGTTGCAT ATATTCGCTC CAATCAGATT
    ATGGGCTGGG GAGAAAAAGC GATCGAAATA CGCTCGGTAG AAACTGGACA CTTGGACGGT
    GTGTTCATGC ACAAAAGGGC ACAAAGACTC AAGTTCTTAT GTGAACGCAA TGACAAGGTT
    TTCTTTGCCT CCGTACGGTC GGGTGGAAGC AGTCAAGTTT ATTTCATGAC CCTTGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133293.1
    CDS680..4600
    Translation

    Target ORF information:

    RefSeq Version XM_015277807.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277807.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACCTGT GAGAACAACA TCCCGATCAC CAGTCCTGTC CCGTCGTGAT
    TCTCCACTGC AGGGCAGTGG GCAGCAAAAT AACCAAGCAG GTCAAAGAAA CTCCACGAGC
    AATATAGAGC CTCGTCTGCT GTGGGAAAGG GTGGAGAAGC TTGTACCAAG ACCAGGCAGT
    GGCAGTTCTT CAGGTTCAAG CAACTCCGGC TCACAGCCTG GTTCCCACCC TGGCTCTCAG
    AGTGGGTCTG GAGAGCGGTT CCGGATGAGA TCATCATCCA AATCAGAGGG TTCACCATCG
    CAGCGTCATG AAAGTGCACC TAAAAAGCCT GAAGAGAAAA AGGAGGTCTT CAGACCTATC
    AAGCCTGCTG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGAGTA CAGTTGACAA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCCTCTTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTAACTA CTAATGAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGTGGGACA
    ACAGTGACTT CTGTGGTAGG ATTTTCTAGT GAAGCAATGA GAACAGAGGC AATAAGGCAA
    GACCCTACAC GGAAAGGATC AGTTGTCAAC GTGAATCCTA CAAACACAAG ACCACAGAGC
    GATACCCCAG AGATCCGCAA ATACAAGAAA AGATTCAATT CTGAGATACT GTGTGCTGCC
    TTATGGGGAG TGAACTTGTT AGTGGGCACA GAAAGTGGTC TGATGCTGCT AGACAGAAGT
    GGTCAGGGGA AGGTTTATCC GCTCATCAAT CGAAGAAGAT TCCAGCAAAT GGATGTACTT
    GAAGGGCTAA ATGTCTTGGT GACAATTTCT GGTAAGAAGA ACAAGTTGCG AGTTTACTAC
    TTGTCCTGGT TGAGAAATAA AATCCTTCAC AATGATCCCG AAGTAGAAAA GAAACAGGGT
    TGGACAACGG TGGGTGACTT GGAGGGCTGT GTGCACTATA AAGTTGTAAA ATATGAGAGA
    ATCAAATTCT TGGTAATTGC GTTGAAGAGT TCTGTGGAAG TCTATGCATG GGCGCCAAAG
    CCATACCACA AATTTATGGC CTTTAAGTCA TTTGGTGAAT TGGTACATAA GCCATTGCTG
    GTGGATCTAA CTGTAGAAGA GGGTCAGAGA CTAAAAGTCA TTTATGGCTC CTGTGCTGGC
    TTCCATGCTG TTGACGTGGA TTCGGGATCA GTCTACGATA TTTATCTACC AACGCATATC
    CAGAGCAGTA TCCAACCTCA CGCAATCATA ATTCTCCCAA ATACGGATGG AATGGAGCTT
    CTGGTCTGTT ATGAGGATGA AGGAGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT
    GTGGTTCTGC AGTGGGGAGA AATGCCAACT TCAGTTGCAT ATATTCGCTC CAATCAGATT
    ATGGGCTGGG GAGAAAAAGC GATCGAAATA CGCTCGGTAG AAACTGGACA CTTGGACGGT
    GTGTTCATGC ACAAAAGGGC ACAAAGACTC AAGTTCTTAT GTGAACGCAA TGACAAGGTT
    TTCTTTGCCT CCGTACGGTC GGGTGGAAGC AGTCAAGTTT ATTTCATGAC CCTTGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143058
    Clone ID Related Accession (Same CDS sequence) XM_015277808.2
    Accession Version XM_015277808.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277808.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACCTGT GAGAACAACA TCCCGATCAC CAGTCCTGTC CCGTCGTGAT
    TCTCCACTGC AGGGCAGTGG GCAGCAAAAT AACCAAGCAG GTCAAAGAAA CTCCACGAGC
    AATATAGAGC CTCGTCTGCT GTGGGAAAGG GTGGAGAAGC TTGTACCAAG ACCAGGCAGT
    GGCAGTTCTT CAGGTTCAAG CAACTCCGGC TCACAGCCTG GTTCCCACCC TGGCTCTCAG
    AGTGGGTCTG GAGAGCGGTT CCGGATGAGA TCATCATCCA AATCAGAGGG TTCACCATCG
    CAGCGTCATG AAAGTGCACC TAAAAAGCCT GAAGAGAAAA AGGAGGTCTT CAGACCTATC
    AAGCCTGCTG GAGAAGTGGA TTTAACTGCA TTGGCAAAGG AGCTGCGAGC AGTAGAGGAT
    GTGCGACCTC CACATAAAGT GACGGATTAT TCGTCCTCAA GTGAGGAGTC AGGGACGACA
    GATGAAGAAG ATGATGATAT GGAACAAGAA GGAGCAGATG AATCTACTTC TGGACCAGAT
    GATGTCCGAG CAGTGTCAAC ACTGAACTTG AGCAATGGTG AAACAGAGTC TGTAAAAACT
    ATGATTGTCC ATGATGATGT GGAGAGTGAA CCTGCCTTGA CTCCTTCTAA GGAGGGCACC
    TTAATTGTCC GACAGACTCA GTCCGCTAGT AACACACTCC AGAAACACAA ATCTTCCTCC
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG TGGGACAACA
    GTGACTTCTG TGGTAGGATT TTCTAGTGAA GCAATGAGAA CAGAGGCAAT AAGGCAAGAC
    CCTACACGGA AAGGATCAGT TGTCAACGTG AATCCTACAA ACACAAGACC ACAGAGCGAT
    ACCCCAGAGA TCCGCAAATA CAAGAAAAGA TTCAATTCTG AGATACTGTG TGCTGCCTTA
    TGGGGAGTGA ACTTGTTAGT GGGCACAGAA AGTGGTCTGA TGCTGCTAGA CAGAAGTGGT
    CAGGGGAAGG TTTATCCGCT CATCAATCGA AGAAGATTCC AGCAAATGGA TGTACTTGAA
    GGGCTAAATG TCTTGGTGAC AATTTCTGGT AAGAAGAACA AGTTGCGAGT TTACTACTTG
    TCCTGGTTGA GAAATAAAAT CCTTCACAAT GATCCCGAAG TAGAAAAGAA ACAGGGTTGG
    ACAACGGTGG GTGACTTGGA GGGCTGTGTG CACTATAAAG TTGTAAAATA TGAGAGAATC
    AAATTCTTGG TAATTGCGTT GAAGAGTTCT GTGGAAGTCT ATGCATGGGC GCCAAAGCCA
    TACCACAAAT TTATGGCCTT TAAGTCATTT GGTGAATTGG TACATAAGCC ATTGCTGGTG
    GATCTAACTG TAGAAGAGGG TCAGAGACTA AAAGTCATTT ATGGCTCCTG TGCTGGCTTC
    CATGCTGTTG ACGTGGATTC GGGATCAGTC TACGATATTT ATCTACCAAC GCATATCCAG
    AGCAGTATCC AACCTCACGC AATCATAATT CTCCCAAATA CGGATGGAAT GGAGCTTCTG
    GTCTGTTATG AGGATGAAGG AGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTG
    GTTCTGCAGT GGGGAGAAAT GCCAACTTCA GTTGCATATA TTCGCTCCAA TCAGATTATG
    GGCTGGGGAG AAAAAGCGAT CGAAATACGC TCGGTAGAAA CTGGACACTT GGACGGTGTG
    TTCATGCACA AAAGGGCACA AAGACTCAAG TTCTTATGTG AACGCAATGA CAAGGTTTTC
    TTTGCCTCCG TACGGTCGGG TGGAAGCAGT CAAGTTTATT TCATGACCCT TGGCAGGACT
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133294.1
    CDS682..4419
    Translation

    Target ORF information:

    RefSeq Version XM_015277808.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277808.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACCTGT GAGAACAACA TCCCGATCAC CAGTCCTGTC CCGTCGTGAT
    TCTCCACTGC AGGGCAGTGG GCAGCAAAAT AACCAAGCAG GTCAAAGAAA CTCCACGAGC
    AATATAGAGC CTCGTCTGCT GTGGGAAAGG GTGGAGAAGC TTGTACCAAG ACCAGGCAGT
    GGCAGTTCTT CAGGTTCAAG CAACTCCGGC TCACAGCCTG GTTCCCACCC TGGCTCTCAG
    AGTGGGTCTG GAGAGCGGTT CCGGATGAGA TCATCATCCA AATCAGAGGG TTCACCATCG
    CAGCGTCATG AAAGTGCACC TAAAAAGCCT GAAGAGAAAA AGGAGGTCTT CAGACCTATC
    AAGCCTGCTG GAGAAGTGGA TTTAACTGCA TTGGCAAAGG AGCTGCGAGC AGTAGAGGAT
    GTGCGACCTC CACATAAAGT GACGGATTAT TCGTCCTCAA GTGAGGAGTC AGGGACGACA
    GATGAAGAAG ATGATGATAT GGAACAAGAA GGAGCAGATG AATCTACTTC TGGACCAGAT
    GATGTCCGAG CAGTGTCAAC ACTGAACTTG AGCAATGGTG AAACAGAGTC TGTAAAAACT
    ATGATTGTCC ATGATGATGT GGAGAGTGAA CCTGCCTTGA CTCCTTCTAA GGAGGGCACC
    TTAATTGTCC GACAGACTCA GTCCGCTAGT AACACACTCC AGAAACACAA ATCTTCCTCC
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG TGGGACAACA
    GTGACTTCTG TGGTAGGATT TTCTAGTGAA GCAATGAGAA CAGAGGCAAT AAGGCAAGAC
    CCTACACGGA AAGGATCAGT TGTCAACGTG AATCCTACAA ACACAAGACC ACAGAGCGAT
    ACCCCAGAGA TCCGCAAATA CAAGAAAAGA TTCAATTCTG AGATACTGTG TGCTGCCTTA
    TGGGGAGTGA ACTTGTTAGT GGGCACAGAA AGTGGTCTGA TGCTGCTAGA CAGAAGTGGT
    CAGGGGAAGG TTTATCCGCT CATCAATCGA AGAAGATTCC AGCAAATGGA TGTACTTGAA
    GGGCTAAATG TCTTGGTGAC AATTTCTGGT AAGAAGAACA AGTTGCGAGT TTACTACTTG
    TCCTGGTTGA GAAATAAAAT CCTTCACAAT GATCCCGAAG TAGAAAAGAA ACAGGGTTGG
    ACAACGGTGG GTGACTTGGA GGGCTGTGTG CACTATAAAG TTGTAAAATA TGAGAGAATC
    AAATTCTTGG TAATTGCGTT GAAGAGTTCT GTGGAAGTCT ATGCATGGGC GCCAAAGCCA
    TACCACAAAT TTATGGCCTT TAAGTCATTT GGTGAATTGG TACATAAGCC ATTGCTGGTG
    GATCTAACTG TAGAAGAGGG TCAGAGACTA AAAGTCATTT ATGGCTCCTG TGCTGGCTTC
    CATGCTGTTG ACGTGGATTC GGGATCAGTC TACGATATTT ATCTACCAAC GCATATCCAG
    AGCAGTATCC AACCTCACGC AATCATAATT CTCCCAAATA CGGATGGAAT GGAGCTTCTG
    GTCTGTTATG AGGATGAAGG AGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTG
    GTTCTGCAGT GGGGAGAAAT GCCAACTTCA GTTGCATATA TTCGCTCCAA TCAGATTATG
    GGCTGGGGAG AAAAAGCGAT CGAAATACGC TCGGTAGAAA CTGGACACTT GGACGGTGTG
    TTCATGCACA AAAGGGCACA AAGACTCAAG TTCTTATGTG AACGCAATGA CAAGGTTTTC
    TTTGCCTCCG TACGGTCGGG TGGAAGCAGT CAAGTTTATT TCATGACCCT TGGCAGGACT
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa143059
    Clone ID Related Accession (Same CDS sequence) XM_015277809.2
    Accession Version XM_015277809.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACCTGT GAGAACAACA TCCCGATCAC CAGTCCTGTC CCGTCGTGAT
    TCTCCACTGC AGGGCAGTGG GCAGCAAAAT AACCAAGCAG GTCAAAGAAA CTCCACGAGC
    AATATAGAGC CTCGTCTGCT GTGGGAAAGG GTGGAGAAGC TTGTACCAAG ACCAGGCAGT
    GGCAGTTCTT CAGGTTCAAG CAACTCCGGC TCACAGCCTG GTTCCCACCC TGGCTCTCAG
    AGTGGGTCTG GAGAGCGGTT CCGGATGAGA TCATCATCCA AATCAGAGGG TTCACCATCG
    CAGCGTCATG AAAGTGCACC TAAAAAGCCT GAAGAGAAAA AGGAGGTCTT CAGACCTATC
    AAGCCTGCTG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGGACAAC AGTGACTTCT
    GTGGTAGGAT TTTCTAGTGA AGCAATGAGA ACAGAGGCAA TAAGGCAAGA CCCTACACGG
    AAAGGATCAG TTGTCAACGT GAATCCTACA AACACAAGAC CACAGAGCGA TACCCCAGAG
    ATCCGCAAAT ACAAGAAAAG ATTCAATTCT GAGATACTGT GTGCTGCCTT ATGGGGAGTG
    AACTTGTTAG TGGGCACAGA AAGTGGTCTG ATGCTGCTAG ACAGAAGTGG TCAGGGGAAG
    GTTTATCCGC TCATCAATCG AAGAAGATTC CAGCAAATGG ATGTACTTGA AGGGCTAAAT
    GTCTTGGTGA CAATTTCTGG TAAGAAGAAC AAGTTGCGAG TTTACTACTT GTCCTGGTTG
    AGAAATAAAA TCCTTCACAA TGATCCCGAA GTAGAAAAGA AACAGGGTTG GACAACGGTG
    GGTGACTTGG AGGGCTGTGT GCACTATAAA GTTGTAAAAT ATGAGAGAAT CAAATTCTTG
    GTAATTGCGT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CGCCAAAGCC ATACCACAAA
    TTTATGGCCT TTAAGTCATT TGGTGAATTG GTACATAAGC CATTGCTGGT GGATCTAACT
    GTAGAAGAGG GTCAGAGACT AAAAGTCATT TATGGCTCCT GTGCTGGCTT CCATGCTGTT
    GACGTGGATT CGGGATCAGT CTACGATATT TATCTACCAA CGCATATCCA GAGCAGTATC
    CAACCTCACG CAATCATAAT TCTCCCAAAT ACGGATGGAA TGGAGCTTCT GGTCTGTTAT
    GAGGATGAAG GAGTTTATGT AAACACATAT GGAAGGATCA CCAAGGATGT GGTTCTGCAG
    TGGGGAGAAA TGCCAACTTC AGTTGCATAT ATTCGCTCCA ATCAGATTAT GGGCTGGGGA
    GAAAAAGCGA TCGAAATACG CTCGGTAGAA ACTGGACACT TGGACGGTGT GTTCATGCAC
    AAAAGGGCAC AAAGACTCAA GTTCTTATGT GAACGCAATG ACAAGGTTTT CTTTGCCTCC
    GTACGGTCGG GTGGAAGCAG TCAAGTTTAT TTCATGACCC TTGGCAGGAC TTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133295.1
    CDS681..4409
    Translation

    Target ORF information:

    RefSeq Version XM_015277809.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277809.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAACAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACCTGT GAGAACAACA TCCCGATCAC CAGTCCTGTC CCGTCGTGAT
    TCTCCACTGC AGGGCAGTGG GCAGCAAAAT AACCAAGCAG GTCAAAGAAA CTCCACGAGC
    AATATAGAGC CTCGTCTGCT GTGGGAAAGG GTGGAGAAGC TTGTACCAAG ACCAGGCAGT
    GGCAGTTCTT CAGGTTCAAG CAACTCCGGC TCACAGCCTG GTTCCCACCC TGGCTCTCAG
    AGTGGGTCTG GAGAGCGGTT CCGGATGAGA TCATCATCCA AATCAGAGGG TTCACCATCG
    CAGCGTCATG AAAGTGCACC TAAAAAGCCT GAAGAGAAAA AGGAGGTCTT CAGACCTATC
    AAGCCTGCTG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGGACAAC AGTGACTTCT
    GTGGTAGGAT TTTCTAGTGA AGCAATGAGA ACAGAGGCAA TAAGGCAAGA CCCTACACGG
    AAAGGATCAG TTGTCAACGT GAATCCTACA AACACAAGAC CACAGAGCGA TACCCCAGAG
    ATCCGCAAAT ACAAGAAAAG ATTCAATTCT GAGATACTGT GTGCTGCCTT ATGGGGAGTG
    AACTTGTTAG TGGGCACAGA AAGTGGTCTG ATGCTGCTAG ACAGAAGTGG TCAGGGGAAG
    GTTTATCCGC TCATCAATCG AAGAAGATTC CAGCAAATGG ATGTACTTGA AGGGCTAAAT
    GTCTTGGTGA CAATTTCTGG TAAGAAGAAC AAGTTGCGAG TTTACTACTT GTCCTGGTTG
    AGAAATAAAA TCCTTCACAA TGATCCCGAA GTAGAAAAGA AACAGGGTTG GACAACGGTG
    GGTGACTTGG AGGGCTGTGT GCACTATAAA GTTGTAAAAT ATGAGAGAAT CAAATTCTTG
    GTAATTGCGT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CGCCAAAGCC ATACCACAAA
    TTTATGGCCT TTAAGTCATT TGGTGAATTG GTACATAAGC CATTGCTGGT GGATCTAACT
    GTAGAAGAGG GTCAGAGACT AAAAGTCATT TATGGCTCCT GTGCTGGCTT CCATGCTGTT
    GACGTGGATT CGGGATCAGT CTACGATATT TATCTACCAA CGCATATCCA GAGCAGTATC
    CAACCTCACG CAATCATAAT TCTCCCAAAT ACGGATGGAA TGGAGCTTCT GGTCTGTTAT
    GAGGATGAAG GAGTTTATGT AAACACATAT GGAAGGATCA CCAAGGATGT GGTTCTGCAG
    TGGGGAGAAA TGCCAACTTC AGTTGCATAT ATTCGCTCCA ATCAGATTAT GGGCTGGGGA
    GAAAAAGCGA TCGAAATACG CTCGGTAGAA ACTGGACACT TGGACGGTGT GTTCATGCAC
    AAAAGGGCAC AAAGACTCAA GTTCTTATGT GAACGCAATG ACAAGGTTTT CTTTGCCTCC
    GTACGGTCGG GTGGAAGCAG TCAAGTTTAT TTCATGACCC TTGGCAGGAC TTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30633
    Clone ID Related Accession (Same CDS sequence) XM_015277799.1
    Accession Version XM_015277799.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4161bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763584.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GGAGAAGTGG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGAGTA CAGTTGACAA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCCTCTTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTAACTA CTAATGAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGTGGGACA
    ACAGTGACTT CTGTGGTAGG ATTTTCTAGT GAAGCAATGA GAACAGAGGC AATAAGGCAA
    GACCCTACAC GGAAAGGATC AGTTGTCAAC GTGAATCCTA CAAACACAAG ACCACAGAGC
    GATACCCCAG AGATCCGCAA ATACAAGAAA AGATTCAATT CTGAGATACT GTGTGCTGCC
    TTATGGGGAG TGAACTTGTT AGTGGGCACA GAAAGTGGTC TGATGCTGCT AGACAGAAGT
    GGTCAGGGGA AGGTTTATCC GCTCATCAAT CGAAGAAGAT TCCAGCAAAT GGATGTACTT
    GAAGGGCTAA ATGTCTTGGT GACAATTTCT GGTAAGAAGA ACAAGTTGCG AGTTTACTAC
    TTGTCCTGGT TGAGAAATAA AATCCTTCAC AATGATCCCG AAGTAGAAAA GAAACAGGGT
    TGGACAACGG TGGGTGACTT GGAGGGCTGT GTGCACTATA AAGTTGTAAA ATATGAGAGA
    ATCAAATTCT TGGTAATTGC GTTGAAGAGT TCTGTGGAAG TCTATGCATG GGCGCCAAAG
    CCATACCACA AATTTATGGC CTTTAAGTCA TTTGGTGAAT TGGTACATAA GCCATTGCTG
    GTGGATCTAA CTGTAGAAGA GGGTCAGAGA CTAAAAGTCA TTTATGGCTC CTGTGCTGGC
    TTCCATGCTG TGGACGTGGA TTCGGGATCA GTCTACGATA TTTATCTACC AACACATATC
    CAGAGCAGTA TCCAACCTCA CGCAATCATA ATTCTCCCAA ATACGGATGG AATGGAGCTT
    CTGGTCTGTT ATGAGGATGA AGGAGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT
    GTGGTTCTGC AGTGGGGAGA AATGCCAACT TCAGTTGCAT ATATTCGCTC CAATCAGATT
    ATGGGCTGGG GAGAAAAAGC GATCGAAATA CGCTCGGTAG AAACTGGACA CTTGGACGGT
    GTGTTCATGC ACAAAAGGGC ACAAAGACTC AAGTTCTTAT GTGAACGCAA TGACAAGGTT
    TTCTTTGCCT CCGTACGGTC GGGTGGAAGC AGTCAAGTTT ATTTCATGAC CCTTGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133285.1
    CDS33..4193
    Translation

    Target ORF information:

    RefSeq Version XM_015277799.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277799.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GGAGAAGTGG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGAGTA CAGTTGACAA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCCTCTTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTAACTA CTAATGAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGTGGGACA
    ACAGTGACTT CTGTGGTAGG ATTTTCTAGT GAAGCAATGA GAACAGAGGC AATAAGGCAA
    GACCCTACAC GGAAAGGATC AGTTGTCAAC GTGAATCCTA CAAACACAAG ACCACAGAGC
    GATACCCCAG AGATCCGCAA ATACAAGAAA AGATTCAATT CTGAGATACT GTGTGCTGCC
    TTATGGGGAG TGAACTTGTT AGTGGGCACA GAAAGTGGTC TGATGCTGCT AGACAGAAGT
    GGTCAGGGGA AGGTTTATCC GCTCATCAAT CGAAGAAGAT TCCAGCAAAT GGATGTACTT
    GAAGGGCTAA ATGTCTTGGT GACAATTTCT GGTAAGAAGA ACAAGTTGCG AGTTTACTAC
    TTGTCCTGGT TGAGAAATAA AATCCTTCAC AATGATCCCG AAGTAGAAAA GAAACAGGGT
    TGGACAACGG TGGGTGACTT GGAGGGCTGT GTGCACTATA AAGTTGTAAA ATATGAGAGA
    ATCAAATTCT TGGTAATTGC GTTGAAGAGT TCTGTGGAAG TCTATGCATG GGCGCCAAAG
    CCATACCACA AATTTATGGC CTTTAAGTCA TTTGGTGAAT TGGTACATAA GCCATTGCTG
    GTGGATCTAA CTGTAGAAGA GGGTCAGAGA CTAAAAGTCA TTTATGGCTC CTGTGCTGGC
    TTCCATGCTG TGGACGTGGA TTCGGGATCA GTCTACGATA TTTATCTACC AACACATATC
    CAGAGCAGTA TCCAACCTCA CGCAATCATA ATTCTCCCAA ATACGGATGG AATGGAGCTT
    CTGGTCTGTT ATGAGGATGA AGGAGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT
    GTGGTTCTGC AGTGGGGAGA AATGCCAACT TCAGTTGCAT ATATTCGCTC CAATCAGATT
    ATGGGCTGGG GAGAAAAAGC GATCGAAATA CGCTCGGTAG AAACTGGACA CTTGGACGGT
    GTGTTCATGC ACAAAAGGGC ACAAAGACTC AAGTTCTTAT GTGAACGCAA TGACAAGGTT
    TTCTTTGCCT CCGTACGGTC GGGTGGAAGC AGTCAAGTTT ATTTCATGAC CCTTGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30634
    Clone ID Related Accession (Same CDS sequence) XM_015277800.1
    Accession Version XM_015277800.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763584.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GGAGAAGTGG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGAGTA CAGTTGACAA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCCTCTTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTAACTA CTAATGAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGTGGGACA
    ACAGTGACTT CTGTGGGATT TTCTAGTGAA GCAATGAGAA CAGAGGCAAT AAGGCAAGAC
    CCTACACGGA AAGGATCAGT TGTCAACGTG AATCCTACAA ACACAAGACC ACAGAGCGAT
    ACCCCAGAGA TCCGCAAATA CAAGAAAAGA TTCAATTCTG AGATACTGTG TGCTGCCTTA
    TGGGGAGTGA ACTTGTTAGT GGGCACAGAA AGTGGTCTGA TGCTGCTAGA CAGAAGTGGT
    CAGGGGAAGG TTTATCCGCT CATCAATCGA AGAAGATTCC AGCAAATGGA TGTACTTGAA
    GGGCTAAATG TCTTGGTGAC AATTTCTGGT AAGAAGAACA AGTTGCGAGT TTACTACTTG
    TCCTGGTTGA GAAATAAAAT CCTTCACAAT GATCCCGAAG TAGAAAAGAA ACAGGGTTGG
    ACAACGGTGG GTGACTTGGA GGGCTGTGTG CACTATAAAG TTGTAAAATA TGAGAGAATC
    AAATTCTTGG TAATTGCGTT GAAGAGTTCT GTGGAAGTCT ATGCATGGGC GCCAAAGCCA
    TACCACAAAT TTATGGCCTT TAAGTCATTT GGTGAATTGG TACATAAGCC ATTGCTGGTG
    GATCTAACTG TAGAAGAGGG TCAGAGACTA AAAGTCATTT ATGGCTCCTG TGCTGGCTTC
    CATGCTGTGG ACGTGGATTC GGGATCAGTC TACGATATTT ATCTACCAAC ACATATCCAG
    AGCAGTATCC AACCTCACGC AATCATAATT CTCCCAAATA CGGATGGAAT GGAGCTTCTG
    GTCTGTTATG AGGATGAAGG AGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTG
    GTTCTGCAGT GGGGAGAAAT GCCAACTTCA GTTGCATATA TTCGCTCCAA TCAGATTATG
    GGCTGGGGAG AAAAAGCGAT CGAAATACGC TCGGTAGAAA CTGGACACTT GGACGGTGTG
    TTCATGCACA AAAGGGCACA AAGACTCAAG TTCTTATGTG AACGCAATGA CAAGGTTTTC
    TTTGCCTCCG TACGGTCGGG TGGAAGCAGT CAAGTTTATT TCATGACCCT TGGCAGGACT
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133286.1
    CDS33..4190
    Translation

    Target ORF information:

    RefSeq Version XM_015277800.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277800.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GGAGAAGTGG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGAGTA CAGTTGACAA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCCTCTTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTAACTA CTAATGAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGTGGGACA
    ACAGTGACTT CTGTGGGATT TTCTAGTGAA GCAATGAGAA CAGAGGCAAT AAGGCAAGAC
    CCTACACGGA AAGGATCAGT TGTCAACGTG AATCCTACAA ACACAAGACC ACAGAGCGAT
    ACCCCAGAGA TCCGCAAATA CAAGAAAAGA TTCAATTCTG AGATACTGTG TGCTGCCTTA
    TGGGGAGTGA ACTTGTTAGT GGGCACAGAA AGTGGTCTGA TGCTGCTAGA CAGAAGTGGT
    CAGGGGAAGG TTTATCCGCT CATCAATCGA AGAAGATTCC AGCAAATGGA TGTACTTGAA
    GGGCTAAATG TCTTGGTGAC AATTTCTGGT AAGAAGAACA AGTTGCGAGT TTACTACTTG
    TCCTGGTTGA GAAATAAAAT CCTTCACAAT GATCCCGAAG TAGAAAAGAA ACAGGGTTGG
    ACAACGGTGG GTGACTTGGA GGGCTGTGTG CACTATAAAG TTGTAAAATA TGAGAGAATC
    AAATTCTTGG TAATTGCGTT GAAGAGTTCT GTGGAAGTCT ATGCATGGGC GCCAAAGCCA
    TACCACAAAT TTATGGCCTT TAAGTCATTT GGTGAATTGG TACATAAGCC ATTGCTGGTG
    GATCTAACTG TAGAAGAGGG TCAGAGACTA AAAGTCATTT ATGGCTCCTG TGCTGGCTTC
    CATGCTGTGG ACGTGGATTC GGGATCAGTC TACGATATTT ATCTACCAAC ACATATCCAG
    AGCAGTATCC AACCTCACGC AATCATAATT CTCCCAAATA CGGATGGAAT GGAGCTTCTG
    GTCTGTTATG AGGATGAAGG AGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTG
    GTTCTGCAGT GGGGAGAAAT GCCAACTTCA GTTGCATATA TTCGCTCCAA TCAGATTATG
    GGCTGGGGAG AAAAAGCGAT CGAAATACGC TCGGTAGAAA CTGGACACTT GGACGGTGTG
    TTCATGCACA AAAGGGCACA AAGACTCAAG TTCTTATGTG AACGCAATGA CAAGGTTTTC
    TTTGCCTCCG TACGGTCGGG TGGAAGCAGT CAAGTTTATT TCATGACCCT TGGCAGGACT
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30635
    Clone ID Related Accession (Same CDS sequence) XM_015277801.1
    Accession Version XM_015277801.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763584.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GATTTAACTG CATTGGCAAA GGAGCTGCGA GCAGTAGAGG ATGTGCGACC TCCACATAAA
    GTGACGGATT ATTCGTCCTC AAGTGAGGAG TCAGGGACGA CAGATGAAGA AGATGATGAT
    ATGGAACAAG AAGGAGCAGA TGAATCTACT TCTGGACCAG ATGATGTCCG AGCAGTGTCA
    ACACTGAACT TGAGCAATGG TGAAACAGAG TCTGTAAAAA CTATGATTGT CCATGATGAT
    GTGGAGAGTG AACCTGCCTT GACTCCTTCT AAGGAGGGCA CCTTAATTGT CCGACAGAGT
    ACAGTTGACA AAAAGCGTGC CAGCCATCAT GAGAGCAATG GCTTTGCCGG TCGCATTCAC
    CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCCTCTTCC
    CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTAACT
    ACTAATGAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGGAC AACAGTGACT
    TCTGTGGTAG GATTTTCTAG TGAAGCAATG AGAACAGAGG CAATAAGGCA AGACCCTACA
    CGGAAAGGAT CAGTTGTCAA CGTGAATCCT ACAAACACAA GACCACAGAG CGATACCCCA
    GAGATCCGCA AATACAAGAA AAGATTCAAT TCTGAGATAC TGTGTGCTGC CTTATGGGGA
    GTGAACTTGT TAGTGGGCAC AGAAAGTGGT CTGATGCTGC TAGACAGAAG TGGTCAGGGG
    AAGGTTTATC CGCTCATCAA TCGAAGAAGA TTCCAGCAAA TGGATGTACT TGAAGGGCTA
    AATGTCTTGG TGACAATTTC TGGTAAGAAG AACAAGTTGC GAGTTTACTA CTTGTCCTGG
    TTGAGAAATA AAATCCTTCA CAATGATCCC GAAGTAGAAA AGAAACAGGG TTGGACAACG
    GTGGGTGACT TGGAGGGCTG TGTGCACTAT AAAGTTGTAA AATATGAGAG AATCAAATTC
    TTGGTAATTG CGTTGAAGAG TTCTGTGGAA GTCTATGCAT GGGCGCCAAA GCCATACCAC
    AAATTTATGG CCTTTAAGTC ATTTGGTGAA TTGGTACATA AGCCATTGCT GGTGGATCTA
    ACTGTAGAAG AGGGTCAGAG ACTAAAAGTC ATTTATGGCT CCTGTGCTGG CTTCCATGCT
    GTGGACGTGG ATTCGGGATC AGTCTACGAT ATTTATCTAC CAACACATAT CCAGAGCAGT
    ATCCAACCTC ACGCAATCAT AATTCTCCCA AATACGGATG GAATGGAGCT TCTGGTCTGT
    TATGAGGATG AAGGAGTTTA TGTAAACACA TATGGAAGGA TCACCAAGGA TGTGGTTCTG
    CAGTGGGGAG AAATGCCAAC TTCAGTTGCA TATATTCGCT CCAATCAGAT TATGGGCTGG
    GGAGAAAAAG CGATCGAAAT ACGCTCGGTA GAAACTGGAC ACTTGGACGG TGTGTTCATG
    CACAAAAGGG CACAAAGACT CAAGTTCTTA TGTGAACGCA ATGACAAGGT TTTCTTTGCC
    TCCGTACGGT CGGGTGGAAG CAGTCAAGTT TATTTCATGA CCCTTGGCAG GACTTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133287.1
    CDS33..4184
    Translation

    Target ORF information:

    RefSeq Version XM_015277801.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277801.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GATTTAACTG CATTGGCAAA GGAGCTGCGA GCAGTAGAGG ATGTGCGACC TCCACATAAA
    GTGACGGATT ATTCGTCCTC AAGTGAGGAG TCAGGGACGA CAGATGAAGA AGATGATGAT
    ATGGAACAAG AAGGAGCAGA TGAATCTACT TCTGGACCAG ATGATGTCCG AGCAGTGTCA
    ACACTGAACT TGAGCAATGG TGAAACAGAG TCTGTAAAAA CTATGATTGT CCATGATGAT
    GTGGAGAGTG AACCTGCCTT GACTCCTTCT AAGGAGGGCA CCTTAATTGT CCGACAGAGT
    ACAGTTGACA AAAAGCGTGC CAGCCATCAT GAGAGCAATG GCTTTGCCGG TCGCATTCAC
    CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCCTCTTCC
    CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTAACT
    ACTAATGAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGTGGGAC AACAGTGACT
    TCTGTGGTAG GATTTTCTAG TGAAGCAATG AGAACAGAGG CAATAAGGCA AGACCCTACA
    CGGAAAGGAT CAGTTGTCAA CGTGAATCCT ACAAACACAA GACCACAGAG CGATACCCCA
    GAGATCCGCA AATACAAGAA AAGATTCAAT TCTGAGATAC TGTGTGCTGC CTTATGGGGA
    GTGAACTTGT TAGTGGGCAC AGAAAGTGGT CTGATGCTGC TAGACAGAAG TGGTCAGGGG
    AAGGTTTATC CGCTCATCAA TCGAAGAAGA TTCCAGCAAA TGGATGTACT TGAAGGGCTA
    AATGTCTTGG TGACAATTTC TGGTAAGAAG AACAAGTTGC GAGTTTACTA CTTGTCCTGG
    TTGAGAAATA AAATCCTTCA CAATGATCCC GAAGTAGAAA AGAAACAGGG TTGGACAACG
    GTGGGTGACT TGGAGGGCTG TGTGCACTAT AAAGTTGTAA AATATGAGAG AATCAAATTC
    TTGGTAATTG CGTTGAAGAG TTCTGTGGAA GTCTATGCAT GGGCGCCAAA GCCATACCAC
    AAATTTATGG CCTTTAAGTC ATTTGGTGAA TTGGTACATA AGCCATTGCT GGTGGATCTA
    ACTGTAGAAG AGGGTCAGAG ACTAAAAGTC ATTTATGGCT CCTGTGCTGG CTTCCATGCT
    GTGGACGTGG ATTCGGGATC AGTCTACGAT ATTTATCTAC CAACACATAT CCAGAGCAGT
    ATCCAACCTC ACGCAATCAT AATTCTCCCA AATACGGATG GAATGGAGCT TCTGGTCTGT
    TATGAGGATG AAGGAGTTTA TGTAAACACA TATGGAAGGA TCACCAAGGA TGTGGTTCTG
    CAGTGGGGAG AAATGCCAAC TTCAGTTGCA TATATTCGCT CCAATCAGAT TATGGGCTGG
    GGAGAAAAAG CGATCGAAAT ACGCTCGGTA GAAACTGGAC ACTTGGACGG TGTGTTCATG
    CACAAAAGGG CACAAAGACT CAAGTTCTTA TGTGAACGCA ATGACAAGGT TTTCTTTGCC
    TCCGTACGGT CGGGTGGAAG CAGTCAAGTT TATTTCATGA CCCTTGGCAG GACTTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30636
    Clone ID Related Accession (Same CDS sequence) XM_015277802.1
    Accession Version XM_015277802.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763584.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAATA CAGATGGCGA GAGATGGAGG AGCATCGACA GGCAGAGAGG
    CTCCAGCGCC AGTTGCAGCA GGAGCAAGCC TACCTCCTGT CACTGCAGCA TGATCACAGG
    CGGCAGCAGC AGCAGCAACA GCAGCAGCAG CAGCAACAGC AGCAGCAGCA AGAAAGAAAT
    AAACAAAGCT ATCATACCCC TGAGCCTAAA TCCCACTATG AGCCTGCTGA AAGAGCGCGT
    GAGGTGGAGG AGAGATTTAG AAAAACGAAT CAGGGCTCCC CTGAAGCACA GTCTAAGCAG
    ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT TCAAGATCAG AGTCCTTTTC CAATGGAAAC
    TCAGAACCTG CTCAGCCTGC CCTGCAGAGG CCAATGGAGC CCCAAGTACA GTGGTCCCAT
    CTGGCATCTC TCAAGAACAA TGTTTCCCCT GTCTCGCGAT CCCATTCCTT CAGTGACCCT
    TCTCCCAAAT TTGCTCATCA CCATCTTCGT TCCCAGGACC CATATCCACC TTCACGCAGT
    GAAGTGCTAA ATCAGAGTTC TGACTCTAAG TCGGAGGTAC CCGACCCCAC CCAAAAGGCT
    TGGGCTAGAT CAGACAGTGA CGAGGTGCCT CCAAGGGTAC CTGTGAGAAC AACATCCCGA
    TCACCAGTCC TGTCCCGTCG TGATTCTCCA CTGCAGGGCA GTGGGCAGCA AAATAACCAA
    GCAGGTCAAA GAAACTCCAC GAGCAATATA GAGCCTCGTC TGCTGTGGGA AAGGGTGGAG
    AAGCTTGTAC CAAGACCAGG CAGTGGCAGT TCTTCAGGTT CAAGCAACTC CGGCTCACAG
    CCTGGTTCCC ACCCTGGCTC TCAGAGTGGG TCTGGAGAGC GGTTCCGGAT GAGATCATCA
    TCCAAATCAG AGGGTTCACC ATCGCAGCGT CATGAAAGTG CACCTAAAAA GCCTGAAGAG
    AAAAAGGAGG TCTTCAGACC TATCAAGCCT GCTGGAGAAG TGGATTTAAC TGCATTGGCA
    AAGGAGCTGC GAGCAGTAGA GGATGTGCGA CCTCCACATA AAGTGACGGA TTATTCGTCC
    TCAAGTGAGG AGTCAGGGAC GACAGATGAA GAAGATGATG ATATGGAACA AGAAGGAGCA
    GATGAATCTA CTTCTGGACC AGATGATGTC CGAGCAGTGT CAACACTGAA CTTGAGCAAT
    GGTGAAACAG AGTCTGTAAA AACTATGATT GTCCATGATG ATGTGGAGAG TGAACCTGCC
    TTGACTCCTT CTAAGGAGGG CACCTTAATT GTCCGACAGA GTACAGTTGA CAAAAAGCGT
    GCCAGCCATC ATGAGAGCAA TGGCTTTGCC GGTCGCATTC ACCTCTTGCC AGATCTCTTA
    CAGCAAAGCC ATTCCTCCTC CACTTCCTCC ACCTCCTCTT CCCCATCCTC CAGCCAGCCG
    ACACCCACCA TGTCCCCACA GACACCCCAG GACAAGCTAA CTACTAATGA GACTCAGTCC
    GCTAGTAACA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC
    AGATTACTAC AGATTTCTCC ATCTAGTGGG ACAACAGTGA CTTCTGTGGT AGGATTTTCT
    AGTGAAGCAA TGAGAACAGA GGCAATAAGG CAAGACCCTA CACGGAAAGG ATCAGTTGTC
    AACGTGAATC CTACAAACAC AAGACCACAG AGCGATACCC CAGAGATCCG CAAATACAAG
    AAAAGATTCA ATTCTGAGAT ACTGTGTGCT GCCTTATGGG GAGTGAACTT GTTAGTGGGC
    ACAGAAAGTG GTCTGATGCT GCTAGACAGA AGTGGTCAGG GGAAGGTTTA TCCGCTCATC
    AATCGAAGAA GATTCCAGCA AATGGATGTA CTTGAAGGGC TAAATGTCTT GGTGACAATT
    TCTGGTAAGA AGAACAAGTT GCGAGTTTAC TACTTGTCCT GGTTGAGAAA TAAAATCCTT
    CACAATGATC CCGAAGTAGA AAAGAAACAG GGTTGGACAA CGGTGGGTGA CTTGGAGGGC
    TGTGTGCACT ATAAAGTTGT AAAATATGAG AGAATCAAAT TCTTGGTAAT TGCGTTGAAG
    AGTTCTGTGG AAGTCTATGC ATGGGCGCCA AAGCCATACC ACAAATTTAT GGCCTTTAAG
    TCATTTGGTG AATTGGTACA TAAGCCATTG CTGGTGGATC TAACTGTAGA AGAGGGTCAG
    AGACTAAAAG TCATTTATGG CTCCTGTGCT GGCTTCCATG CTGTGGACGT GGATTCGGGA
    TCAGTCTACG ATATTTATCT ACCAACACAT ATCCAGAGCA GTATCCAACC TCACGCAATC
    ATAATTCTCC CAAATACGGA TGGAATGGAG CTTCTGGTCT GTTATGAGGA TGAAGGAGTT
    TATGTAAACA CATATGGAAG GATCACCAAG GATGTGGTTC TGCAGTGGGG AGAAATGCCA
    ACTTCAGTTG CATATATTCG CTCCAATCAG ATTATGGGCT GGGGAGAAAA AGCGATCGAA
    ATACGCTCGG TAGAAACTGG ACACTTGGAC GGTGTGTTCA TGCACAAAAG GGCACAAAGA
    CTCAAGTTCT TATGTGAACG CAATGACAAG GTTTTCTTTG CCTCCGTACG GTCGGGTGGA
    AGCAGTCAAG TTTATTTCAT GACCCTTGGC AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133288.1
    CDS33..4106
    Translation

    Target ORF information:

    RefSeq Version XM_015277802.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277802.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAATA CAGATGGCGA GAGATGGAGG AGCATCGACA GGCAGAGAGG
    CTCCAGCGCC AGTTGCAGCA GGAGCAAGCC TACCTCCTGT CACTGCAGCA TGATCACAGG
    CGGCAGCAGC AGCAGCAACA GCAGCAGCAG CAGCAACAGC AGCAGCAGCA AGAAAGAAAT
    AAACAAAGCT ATCATACCCC TGAGCCTAAA TCCCACTATG AGCCTGCTGA AAGAGCGCGT
    GAGGTGGAGG AGAGATTTAG AAAAACGAAT CAGGGCTCCC CTGAAGCACA GTCTAAGCAG
    ATGGGCAAAG TGTTGGAGCC ACCAGTGCCT TCAAGATCAG AGTCCTTTTC CAATGGAAAC
    TCAGAACCTG CTCAGCCTGC CCTGCAGAGG CCAATGGAGC CCCAAGTACA GTGGTCCCAT
    CTGGCATCTC TCAAGAACAA TGTTTCCCCT GTCTCGCGAT CCCATTCCTT CAGTGACCCT
    TCTCCCAAAT TTGCTCATCA CCATCTTCGT TCCCAGGACC CATATCCACC TTCACGCAGT
    GAAGTGCTAA ATCAGAGTTC TGACTCTAAG TCGGAGGTAC CCGACCCCAC CCAAAAGGCT
    TGGGCTAGAT CAGACAGTGA CGAGGTGCCT CCAAGGGTAC CTGTGAGAAC AACATCCCGA
    TCACCAGTCC TGTCCCGTCG TGATTCTCCA CTGCAGGGCA GTGGGCAGCA AAATAACCAA
    GCAGGTCAAA GAAACTCCAC GAGCAATATA GAGCCTCGTC TGCTGTGGGA AAGGGTGGAG
    AAGCTTGTAC CAAGACCAGG CAGTGGCAGT TCTTCAGGTT CAAGCAACTC CGGCTCACAG
    CCTGGTTCCC ACCCTGGCTC TCAGAGTGGG TCTGGAGAGC GGTTCCGGAT GAGATCATCA
    TCCAAATCAG AGGGTTCACC ATCGCAGCGT CATGAAAGTG CACCTAAAAA GCCTGAAGAG
    AAAAAGGAGG TCTTCAGACC TATCAAGCCT GCTGGAGAAG TGGATTTAAC TGCATTGGCA
    AAGGAGCTGC GAGCAGTAGA GGATGTGCGA CCTCCACATA AAGTGACGGA TTATTCGTCC
    TCAAGTGAGG AGTCAGGGAC GACAGATGAA GAAGATGATG ATATGGAACA AGAAGGAGCA
    GATGAATCTA CTTCTGGACC AGATGATGTC CGAGCAGTGT CAACACTGAA CTTGAGCAAT
    GGTGAAACAG AGTCTGTAAA AACTATGATT GTCCATGATG ATGTGGAGAG TGAACCTGCC
    TTGACTCCTT CTAAGGAGGG CACCTTAATT GTCCGACAGA GTACAGTTGA CAAAAAGCGT
    GCCAGCCATC ATGAGAGCAA TGGCTTTGCC GGTCGCATTC ACCTCTTGCC AGATCTCTTA
    CAGCAAAGCC ATTCCTCCTC CACTTCCTCC ACCTCCTCTT CCCCATCCTC CAGCCAGCCG
    ACACCCACCA TGTCCCCACA GACACCCCAG GACAAGCTAA CTACTAATGA GACTCAGTCC
    GCTAGTAACA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC
    AGATTACTAC AGATTTCTCC ATCTAGTGGG ACAACAGTGA CTTCTGTGGT AGGATTTTCT
    AGTGAAGCAA TGAGAACAGA GGCAATAAGG CAAGACCCTA CACGGAAAGG ATCAGTTGTC
    AACGTGAATC CTACAAACAC AAGACCACAG AGCGATACCC CAGAGATCCG CAAATACAAG
    AAAAGATTCA ATTCTGAGAT ACTGTGTGCT GCCTTATGGG GAGTGAACTT GTTAGTGGGC
    ACAGAAAGTG GTCTGATGCT GCTAGACAGA AGTGGTCAGG GGAAGGTTTA TCCGCTCATC
    AATCGAAGAA GATTCCAGCA AATGGATGTA CTTGAAGGGC TAAATGTCTT GGTGACAATT
    TCTGGTAAGA AGAACAAGTT GCGAGTTTAC TACTTGTCCT GGTTGAGAAA TAAAATCCTT
    CACAATGATC CCGAAGTAGA AAAGAAACAG GGTTGGACAA CGGTGGGTGA CTTGGAGGGC
    TGTGTGCACT ATAAAGTTGT AAAATATGAG AGAATCAAAT TCTTGGTAAT TGCGTTGAAG
    AGTTCTGTGG AAGTCTATGC ATGGGCGCCA AAGCCATACC ACAAATTTAT GGCCTTTAAG
    TCATTTGGTG AATTGGTACA TAAGCCATTG CTGGTGGATC TAACTGTAGA AGAGGGTCAG
    AGACTAAAAG TCATTTATGG CTCCTGTGCT GGCTTCCATG CTGTGGACGT GGATTCGGGA
    TCAGTCTACG ATATTTATCT ACCAACACAT ATCCAGAGCA GTATCCAACC TCACGCAATC
    ATAATTCTCC CAAATACGGA TGGAATGGAG CTTCTGGTCT GTTATGAGGA TGAAGGAGTT
    TATGTAAACA CATATGGAAG GATCACCAAG GATGTGGTTC TGCAGTGGGG AGAAATGCCA
    ACTTCAGTTG CATATATTCG CTCCAATCAG ATTATGGGCT GGGGAGAAAA AGCGATCGAA
    ATACGCTCGG TAGAAACTGG ACACTTGGAC GGTGTGTTCA TGCACAAAAG GGCACAAAGA
    CTCAAGTTCT TATGTGAACG CAATGACAAG GTTTTCTTTG CCTCCGTACG GTCGGGTGGA
    AGCAGTCAAG TTTATTTCAT GACCCTTGGC AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30637
    Clone ID Related Accession (Same CDS sequence) XM_015277803.1
    Accession Version XM_015277803.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3969bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763584.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GGAGAAGTGG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGGACAAC AGTGACTTCT
    GTGGTAGGAT TTTCTAGTGA AGCAATGAGA ACAGAGGCAA TAAGGCAAGA CCCTACACGG
    AAAGGATCAG TTGTCAACGT GAATCCTACA AACACAAGAC CACAGAGCGA TACCCCAGAG
    ATCCGCAAAT ACAAGAAAAG ATTCAATTCT GAGATACTGT GTGCTGCCTT ATGGGGAGTG
    AACTTGTTAG TGGGCACAGA AAGTGGTCTG ATGCTGCTAG ACAGAAGTGG TCAGGGGAAG
    GTTTATCCGC TCATCAATCG AAGAAGATTC CAGCAAATGG ATGTACTTGA AGGGCTAAAT
    GTCTTGGTGA CAATTTCTGG TAAGAAGAAC AAGTTGCGAG TTTACTACTT GTCCTGGTTG
    AGAAATAAAA TCCTTCACAA TGATCCCGAA GTAGAAAAGA AACAGGGTTG GACAACGGTG
    GGTGACTTGG AGGGCTGTGT GCACTATAAA GTTGTAAAAT ATGAGAGAAT CAAATTCTTG
    GTAATTGCGT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CGCCAAAGCC ATACCACAAA
    TTTATGGCCT TTAAGTCATT TGGTGAATTG GTACATAAGC CATTGCTGGT GGATCTAACT
    GTAGAAGAGG GTCAGAGACT AAAAGTCATT TATGGCTCCT GTGCTGGCTT CCATGCTGTG
    GACGTGGATT CGGGATCAGT CTACGATATT TATCTACCAA CACATATCCA GAGCAGTATC
    CAACCTCACG CAATCATAAT TCTCCCAAAT ACGGATGGAA TGGAGCTTCT GGTCTGTTAT
    GAGGATGAAG GAGTTTATGT AAACACATAT GGAAGGATCA CCAAGGATGT GGTTCTGCAG
    TGGGGAGAAA TGCCAACTTC AGTTGCATAT ATTCGCTCCA ATCAGATTAT GGGCTGGGGA
    GAAAAAGCGA TCGAAATACG CTCGGTAGAA ACTGGACACT TGGACGGTGT GTTCATGCAC
    AAAAGGGCAC AAAGACTCAA GTTCTTATGT GAACGCAATG ACAAGGTTTT CTTTGCCTCC
    GTACGGTCGG GTGGAAGCAG TCAAGTTTAT TTCATGACCC TTGGCAGGAC TTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133289.1
    CDS33..4001
    Translation

    Target ORF information:

    RefSeq Version XM_015277803.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277803.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GGAGAAGTGG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGGACAAC AGTGACTTCT
    GTGGTAGGAT TTTCTAGTGA AGCAATGAGA ACAGAGGCAA TAAGGCAAGA CCCTACACGG
    AAAGGATCAG TTGTCAACGT GAATCCTACA AACACAAGAC CACAGAGCGA TACCCCAGAG
    ATCCGCAAAT ACAAGAAAAG ATTCAATTCT GAGATACTGT GTGCTGCCTT ATGGGGAGTG
    AACTTGTTAG TGGGCACAGA AAGTGGTCTG ATGCTGCTAG ACAGAAGTGG TCAGGGGAAG
    GTTTATCCGC TCATCAATCG AAGAAGATTC CAGCAAATGG ATGTACTTGA AGGGCTAAAT
    GTCTTGGTGA CAATTTCTGG TAAGAAGAAC AAGTTGCGAG TTTACTACTT GTCCTGGTTG
    AGAAATAAAA TCCTTCACAA TGATCCCGAA GTAGAAAAGA AACAGGGTTG GACAACGGTG
    GGTGACTTGG AGGGCTGTGT GCACTATAAA GTTGTAAAAT ATGAGAGAAT CAAATTCTTG
    GTAATTGCGT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CGCCAAAGCC ATACCACAAA
    TTTATGGCCT TTAAGTCATT TGGTGAATTG GTACATAAGC CATTGCTGGT GGATCTAACT
    GTAGAAGAGG GTCAGAGACT AAAAGTCATT TATGGCTCCT GTGCTGGCTT CCATGCTGTG
    GACGTGGATT CGGGATCAGT CTACGATATT TATCTACCAA CACATATCCA GAGCAGTATC
    CAACCTCACG CAATCATAAT TCTCCCAAAT ACGGATGGAA TGGAGCTTCT GGTCTGTTAT
    GAGGATGAAG GAGTTTATGT AAACACATAT GGAAGGATCA CCAAGGATGT GGTTCTGCAG
    TGGGGAGAAA TGCCAACTTC AGTTGCATAT ATTCGCTCCA ATCAGATTAT GGGCTGGGGA
    GAAAAAGCGA TCGAAATACG CTCGGTAGAA ACTGGACACT TGGACGGTGT GTTCATGCAC
    AAAAGGGCAC AAAGACTCAA GTTCTTATGT GAACGCAATG ACAAGGTTTT CTTTGCCTCC
    GTACGGTCGG GTGGAAGCAG TCAAGTTTAT TTCATGACCC TTGGCAGGAC TTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30638
    Clone ID Related Accession (Same CDS sequence) XM_015277805.1
    Accession Version XM_015277805.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763584.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GGAGAAGTGG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGGACAAC AGTGACTTCT
    GTGGGATTTT CTAGTGAAGC AATGAGAACA GAGGCAATAA GGCAAGACCC TACACGGAAA
    GGATCAGTTG TCAACGTGAA TCCTACAAAC ACAAGACCAC AGAGCGATAC CCCAGAGATC
    CGCAAATACA AGAAAAGATT CAATTCTGAG ATACTGTGTG CTGCCTTATG GGGAGTGAAC
    TTGTTAGTGG GCACAGAAAG TGGTCTGATG CTGCTAGACA GAAGTGGTCA GGGGAAGGTT
    TATCCGCTCA TCAATCGAAG AAGATTCCAG CAAATGGATG TACTTGAAGG GCTAAATGTC
    TTGGTGACAA TTTCTGGTAA GAAGAACAAG TTGCGAGTTT ACTACTTGTC CTGGTTGAGA
    AATAAAATCC TTCACAATGA TCCCGAAGTA GAAAAGAAAC AGGGTTGGAC AACGGTGGGT
    GACTTGGAGG GCTGTGTGCA CTATAAAGTT GTAAAATATG AGAGAATCAA ATTCTTGGTA
    ATTGCGTTGA AGAGTTCTGT GGAAGTCTAT GCATGGGCGC CAAAGCCATA CCACAAATTT
    ATGGCCTTTA AGTCATTTGG TGAATTGGTA CATAAGCCAT TGCTGGTGGA TCTAACTGTA
    GAAGAGGGTC AGAGACTAAA AGTCATTTAT GGCTCCTGTG CTGGCTTCCA TGCTGTGGAC
    GTGGATTCGG GATCAGTCTA CGATATTTAT CTACCAACAC ATATCCAGAG CAGTATCCAA
    CCTCACGCAA TCATAATTCT CCCAAATACG GATGGAATGG AGCTTCTGGT CTGTTATGAG
    GATGAAGGAG TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTGGT TCTGCAGTGG
    GGAGAAATGC CAACTTCAGT TGCATATATT CGCTCCAATC AGATTATGGG CTGGGGAGAA
    AAAGCGATCG AAATACGCTC GGTAGAAACT GGACACTTGG ACGGTGTGTT CATGCACAAA
    AGGGCACAAA GACTCAAGTT CTTATGTGAA CGCAATGACA AGGTTTTCTT TGCCTCCGTA
    CGGTCGGGTG GAAGCAGTCA AGTTTATTTC ATGACCCTTG GCAGGACTTC TCTTCTGAGC
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133291.1
    CDS33..3998
    Translation

    Target ORF information:

    RefSeq Version XM_015277805.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277805.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACAGTG GTCCCATCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC
    TCGCGATCCC ATTCCTTCAG TGACCCTTCT CCCAAATTTG CTCATCACCA TCTTCGTTCC
    CAGGACCCAT ATCCACCTTC ACGCAGTGAA GTGCTAAATC AGAGTTCTGA CTCTAAGTCG
    GAGGTACCCG ACCCCACCCA AAAGGCTTGG GCTAGATCAG ACAGTGACGA GGTGCCTCCA
    AGGGTACCTG TGAGAACAAC ATCCCGATCA CCAGTCCTGT CCCGTCGTGA TTCTCCACTG
    CAGGGCAGTG GGCAGCAAAA TAACCAAGCA GGTCAAAGAA ACTCCACGAG CAATATAGAG
    CCTCGTCTGC TGTGGGAAAG GGTGGAGAAG CTTGTACCAA GACCAGGCAG TGGCAGTTCT
    TCAGGTTCAA GCAACTCCGG CTCACAGCCT GGTTCCCACC CTGGCTCTCA GAGTGGGTCT
    GGAGAGCGGT TCCGGATGAG ATCATCATCC AAATCAGAGG GTTCACCATC GCAGCGTCAT
    GAAAGTGCAC CTAAAAAGCC TGAAGAGAAA AAGGAGGTCT TCAGACCTAT CAAGCCTGCT
    GGAGAAGTGG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGGACAAC AGTGACTTCT
    GTGGGATTTT CTAGTGAAGC AATGAGAACA GAGGCAATAA GGCAAGACCC TACACGGAAA
    GGATCAGTTG TCAACGTGAA TCCTACAAAC ACAAGACCAC AGAGCGATAC CCCAGAGATC
    CGCAAATACA AGAAAAGATT CAATTCTGAG ATACTGTGTG CTGCCTTATG GGGAGTGAAC
    TTGTTAGTGG GCACAGAAAG TGGTCTGATG CTGCTAGACA GAAGTGGTCA GGGGAAGGTT
    TATCCGCTCA TCAATCGAAG AAGATTCCAG CAAATGGATG TACTTGAAGG GCTAAATGTC
    TTGGTGACAA TTTCTGGTAA GAAGAACAAG TTGCGAGTTT ACTACTTGTC CTGGTTGAGA
    AATAAAATCC TTCACAATGA TCCCGAAGTA GAAAAGAAAC AGGGTTGGAC AACGGTGGGT
    GACTTGGAGG GCTGTGTGCA CTATAAAGTT GTAAAATATG AGAGAATCAA ATTCTTGGTA
    ATTGCGTTGA AGAGTTCTGT GGAAGTCTAT GCATGGGCGC CAAAGCCATA CCACAAATTT
    ATGGCCTTTA AGTCATTTGG TGAATTGGTA CATAAGCCAT TGCTGGTGGA TCTAACTGTA
    GAAGAGGGTC AGAGACTAAA AGTCATTTAT GGCTCCTGTG CTGGCTTCCA TGCTGTGGAC
    GTGGATTCGG GATCAGTCTA CGATATTTAT CTACCAACAC ATATCCAGAG CAGTATCCAA
    CCTCACGCAA TCATAATTCT CCCAAATACG GATGGAATGG AGCTTCTGGT CTGTTATGAG
    GATGAAGGAG TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTGGT TCTGCAGTGG
    GGAGAAATGC CAACTTCAGT TGCATATATT CGCTCCAATC AGATTATGGG CTGGGGAGAA
    AAAGCGATCG AAATACGCTC GGTAGAAACT GGACACTTGG ACGGTGTGTT CATGCACAAA
    AGGGCACAAA GACTCAAGTT CTTATGTGAA CGCAATGACA AGGTTTTCTT TGCCTCCGTA
    CGGTCGGGTG GAAGCAGTCA AGTTTATTTC ATGACCCTTG GCAGGACTTC TCTTCTGAGC
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30639
    Clone ID Related Accession (Same CDS sequence) XM_015277806.1
    Accession Version XM_015277806.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763584.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACCTGT GAGAACAACA TCCCGATCAC CAGTCCTGTC CCGTCGTGAT
    TCTCCACTGC AGGGCAGTGG GCAGCAAAAT AACCAAGCAG GTCAAAGAAA CTCCACGAGC
    AATATAGAGC CTCGTCTGCT GTGGGAAAGG GTGGAGAAGC TTGTACCAAG ACCAGGCAGT
    GGCAGTTCTT CAGGTTCAAG CAACTCCGGC TCACAGCCTG GTTCCCACCC TGGCTCTCAG
    AGTGGGTCTG GAGAGCGGTT CCGGATGAGA TCATCATCCA AATCAGAGGG TTCACCATCG
    CAGCGTCATG AAAGTGCACC TAAAAAGCCT GAAGAGAAAA AGGAGGTCTT CAGACCTATC
    AAGCCTGCTG GAGAAGTGGA TTTAACTGCA TTGGCAAAGG AGCTGCGAGC AGTAGAGGAT
    GTGCGACCTC CACATAAAGT GACGGATTAT TCGTCCTCAA GTGAGGAGTC AGGGACGACA
    GATGAAGAAG ATGATGATAT GGAACAAGAA GGAGCAGATG AATCTACTTC TGGACCAGAT
    GATGTCCGAG CAGTGTCAAC ACTGAACTTG AGCAATGGTG AAACAGAGTC TGTAAAAACT
    ATGATTGTCC ATGATGATGT GGAGAGTGAA CCTGCCTTGA CTCCTTCTAA GGAGGGCACC
    TTAATTGTCC GACAGAGTAC AGTTGACAAA AAGCGTGCCA GCCATCATGA GAGCAATGGC
    TTTGCCGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CCTCTTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTAACTAC TAATGAGACT CAGTCCGCTA GTAACACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT
    AGTGGGACAA CAGTGACTTC TGTGGTAGGA TTTTCTAGTG AAGCAATGAG AACAGAGGCA
    ATAAGGCAAG ACCCTACACG GAAAGGATCA GTTGTCAACG TGAATCCTAC AAACACAAGA
    CCACAGAGCG ATACCCCAGA GATCCGCAAA TACAAGAAAA GATTCAATTC TGAGATACTG
    TGTGCTGCCT TATGGGGAGT GAACTTGTTA GTGGGCACAG AAAGTGGTCT GATGCTGCTA
    GACAGAAGTG GTCAGGGGAA GGTTTATCCG CTCATCAATC GAAGAAGATT CCAGCAAATG
    GATGTACTTG AAGGGCTAAA TGTCTTGGTG ACAATTTCTG GTAAGAAGAA CAAGTTGCGA
    GTTTACTACT TGTCCTGGTT GAGAAATAAA ATCCTTCACA ATGATCCCGA AGTAGAAAAG
    AAACAGGGTT GGACAACGGT GGGTGACTTG GAGGGCTGTG TGCACTATAA AGTTGTAAAA
    TATGAGAGAA TCAAATTCTT GGTAATTGCG TTGAAGAGTT CTGTGGAAGT CTATGCATGG
    GCGCCAAAGC CATACCACAA ATTTATGGCC TTTAAGTCAT TTGGTGAATT GGTACATAAG
    CCATTGCTGG TGGATCTAAC TGTAGAAGAG GGTCAGAGAC TAAAAGTCAT TTATGGCTCC
    TGTGCTGGCT TCCATGCTGT GGACGTGGAT TCGGGATCAG TCTACGATAT TTATCTACCA
    ACACATATCC AGAGCAGTAT CCAACCTCAC GCAATCATAA TTCTCCCAAA TACGGATGGA
    ATGGAGCTTC TGGTCTGTTA TGAGGATGAA GGAGTTTATG TAAACACATA TGGAAGGATC
    ACCAAGGATG TGGTTCTGCA GTGGGGAGAA ATGCCAACTT CAGTTGCATA TATTCGCTCC
    AATCAGATTA TGGGCTGGGG AGAAAAAGCG ATCGAAATAC GCTCGGTAGA AACTGGACAC
    TTGGACGGTG TGTTCATGCA CAAAAGGGCA CAAAGACTCA AGTTCTTATG TGAACGCAAT
    GACAAGGTTT TCTTTGCCTC CGTACGGTCG GGTGGAAGCA GTCAAGTTTA TTTCATGACC
    CTTGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133292.1
    CDS33..3962
    Translation

    Target ORF information:

    RefSeq Version XM_015277806.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277806.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACCTGT GAGAACAACA TCCCGATCAC CAGTCCTGTC CCGTCGTGAT
    TCTCCACTGC AGGGCAGTGG GCAGCAAAAT AACCAAGCAG GTCAAAGAAA CTCCACGAGC
    AATATAGAGC CTCGTCTGCT GTGGGAAAGG GTGGAGAAGC TTGTACCAAG ACCAGGCAGT
    GGCAGTTCTT CAGGTTCAAG CAACTCCGGC TCACAGCCTG GTTCCCACCC TGGCTCTCAG
    AGTGGGTCTG GAGAGCGGTT CCGGATGAGA TCATCATCCA AATCAGAGGG TTCACCATCG
    CAGCGTCATG AAAGTGCACC TAAAAAGCCT GAAGAGAAAA AGGAGGTCTT CAGACCTATC
    AAGCCTGCTG GAGAAGTGGA TTTAACTGCA TTGGCAAAGG AGCTGCGAGC AGTAGAGGAT
    GTGCGACCTC CACATAAAGT GACGGATTAT TCGTCCTCAA GTGAGGAGTC AGGGACGACA
    GATGAAGAAG ATGATGATAT GGAACAAGAA GGAGCAGATG AATCTACTTC TGGACCAGAT
    GATGTCCGAG CAGTGTCAAC ACTGAACTTG AGCAATGGTG AAACAGAGTC TGTAAAAACT
    ATGATTGTCC ATGATGATGT GGAGAGTGAA CCTGCCTTGA CTCCTTCTAA GGAGGGCACC
    TTAATTGTCC GACAGAGTAC AGTTGACAAA AAGCGTGCCA GCCATCATGA GAGCAATGGC
    TTTGCCGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CCTCTTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTAACTAC TAATGAGACT CAGTCCGCTA GTAACACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT
    AGTGGGACAA CAGTGACTTC TGTGGTAGGA TTTTCTAGTG AAGCAATGAG AACAGAGGCA
    ATAAGGCAAG ACCCTACACG GAAAGGATCA GTTGTCAACG TGAATCCTAC AAACACAAGA
    CCACAGAGCG ATACCCCAGA GATCCGCAAA TACAAGAAAA GATTCAATTC TGAGATACTG
    TGTGCTGCCT TATGGGGAGT GAACTTGTTA GTGGGCACAG AAAGTGGTCT GATGCTGCTA
    GACAGAAGTG GTCAGGGGAA GGTTTATCCG CTCATCAATC GAAGAAGATT CCAGCAAATG
    GATGTACTTG AAGGGCTAAA TGTCTTGGTG ACAATTTCTG GTAAGAAGAA CAAGTTGCGA
    GTTTACTACT TGTCCTGGTT GAGAAATAAA ATCCTTCACA ATGATCCCGA AGTAGAAAAG
    AAACAGGGTT GGACAACGGT GGGTGACTTG GAGGGCTGTG TGCACTATAA AGTTGTAAAA
    TATGAGAGAA TCAAATTCTT GGTAATTGCG TTGAAGAGTT CTGTGGAAGT CTATGCATGG
    GCGCCAAAGC CATACCACAA ATTTATGGCC TTTAAGTCAT TTGGTGAATT GGTACATAAG
    CCATTGCTGG TGGATCTAAC TGTAGAAGAG GGTCAGAGAC TAAAAGTCAT TTATGGCTCC
    TGTGCTGGCT TCCATGCTGT GGACGTGGAT TCGGGATCAG TCTACGATAT TTATCTACCA
    ACACATATCC AGAGCAGTAT CCAACCTCAC GCAATCATAA TTCTCCCAAA TACGGATGGA
    ATGGAGCTTC TGGTCTGTTA TGAGGATGAA GGAGTTTATG TAAACACATA TGGAAGGATC
    ACCAAGGATG TGGTTCTGCA GTGGGGAGAA ATGCCAACTT CAGTTGCATA TATTCGCTCC
    AATCAGATTA TGGGCTGGGG AGAAAAAGCG ATCGAAATAC GCTCGGTAGA AACTGGACAC
    TTGGACGGTG TGTTCATGCA CAAAAGGGCA CAAAGACTCA AGTTCTTATG TGAACGCAAT
    GACAAGGTTT TCTTTGCCTC CGTACGGTCG GGTGGAAGCA GTCAAGTTTA TTTCATGACC
    CTTGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30640
    Clone ID Related Accession (Same CDS sequence) XM_015277807.1
    Accession Version XM_015277807.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763584.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACCTGT GAGAACAACA TCCCGATCAC CAGTCCTGTC CCGTCGTGAT
    TCTCCACTGC AGGGCAGTGG GCAGCAAAAT AACCAAGCAG GTCAAAGAAA CTCCACGAGC
    AATATAGAGC CTCGTCTGCT GTGGGAAAGG GTGGAGAAGC TTGTACCAAG ACCAGGCAGT
    GGCAGTTCTT CAGGTTCAAG CAACTCCGGC TCACAGCCTG GTTCCCACCC TGGCTCTCAG
    AGTGGGTCTG GAGAGCGGTT CCGGATGAGA TCATCATCCA AATCAGAGGG TTCACCATCG
    CAGCGTCATG AAAGTGCACC TAAAAAGCCT GAAGAGAAAA AGGAGGTCTT CAGACCTATC
    AAGCCTGCTG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGAGTA CAGTTGACAA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCCTCTTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTAACTA CTAATGAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGTGGGACA
    ACAGTGACTT CTGTGGTAGG ATTTTCTAGT GAAGCAATGA GAACAGAGGC AATAAGGCAA
    GACCCTACAC GGAAAGGATC AGTTGTCAAC GTGAATCCTA CAAACACAAG ACCACAGAGC
    GATACCCCAG AGATCCGCAA ATACAAGAAA AGATTCAATT CTGAGATACT GTGTGCTGCC
    TTATGGGGAG TGAACTTGTT AGTGGGCACA GAAAGTGGTC TGATGCTGCT AGACAGAAGT
    GGTCAGGGGA AGGTTTATCC GCTCATCAAT CGAAGAAGAT TCCAGCAAAT GGATGTACTT
    GAAGGGCTAA ATGTCTTGGT GACAATTTCT GGTAAGAAGA ACAAGTTGCG AGTTTACTAC
    TTGTCCTGGT TGAGAAATAA AATCCTTCAC AATGATCCCG AAGTAGAAAA GAAACAGGGT
    TGGACAACGG TGGGTGACTT GGAGGGCTGT GTGCACTATA AAGTTGTAAA ATATGAGAGA
    ATCAAATTCT TGGTAATTGC GTTGAAGAGT TCTGTGGAAG TCTATGCATG GGCGCCAAAG
    CCATACCACA AATTTATGGC CTTTAAGTCA TTTGGTGAAT TGGTACATAA GCCATTGCTG
    GTGGATCTAA CTGTAGAAGA GGGTCAGAGA CTAAAAGTCA TTTATGGCTC CTGTGCTGGC
    TTCCATGCTG TGGACGTGGA TTCGGGATCA GTCTACGATA TTTATCTACC AACACATATC
    CAGAGCAGTA TCCAACCTCA CGCAATCATA ATTCTCCCAA ATACGGATGG AATGGAGCTT
    CTGGTCTGTT ATGAGGATGA AGGAGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT
    GTGGTTCTGC AGTGGGGAGA AATGCCAACT TCAGTTGCAT ATATTCGCTC CAATCAGATT
    ATGGGCTGGG GAGAAAAAGC GATCGAAATA CGCTCGGTAG AAACTGGACA CTTGGACGGT
    GTGTTCATGC ACAAAAGGGC ACAAAGACTC AAGTTCTTAT GTGAACGCAA TGACAAGGTT
    TTCTTTGCCT CCGTACGGTC GGGTGGAAGC AGTCAAGTTT ATTTCATGAC CCTTGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133293.1
    CDS33..3953
    Translation

    Target ORF information:

    RefSeq Version XM_015277807.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277807.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACCTGT GAGAACAACA TCCCGATCAC CAGTCCTGTC CCGTCGTGAT
    TCTCCACTGC AGGGCAGTGG GCAGCAAAAT AACCAAGCAG GTCAAAGAAA CTCCACGAGC
    AATATAGAGC CTCGTCTGCT GTGGGAAAGG GTGGAGAAGC TTGTACCAAG ACCAGGCAGT
    GGCAGTTCTT CAGGTTCAAG CAACTCCGGC TCACAGCCTG GTTCCCACCC TGGCTCTCAG
    AGTGGGTCTG GAGAGCGGTT CCGGATGAGA TCATCATCCA AATCAGAGGG TTCACCATCG
    CAGCGTCATG AAAGTGCACC TAAAAAGCCT GAAGAGAAAA AGGAGGTCTT CAGACCTATC
    AAGCCTGCTG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGAGTA CAGTTGACAA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCCTCTTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTAACTA CTAATGAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGTGGGACA
    ACAGTGACTT CTGTGGTAGG ATTTTCTAGT GAAGCAATGA GAACAGAGGC AATAAGGCAA
    GACCCTACAC GGAAAGGATC AGTTGTCAAC GTGAATCCTA CAAACACAAG ACCACAGAGC
    GATACCCCAG AGATCCGCAA ATACAAGAAA AGATTCAATT CTGAGATACT GTGTGCTGCC
    TTATGGGGAG TGAACTTGTT AGTGGGCACA GAAAGTGGTC TGATGCTGCT AGACAGAAGT
    GGTCAGGGGA AGGTTTATCC GCTCATCAAT CGAAGAAGAT TCCAGCAAAT GGATGTACTT
    GAAGGGCTAA ATGTCTTGGT GACAATTTCT GGTAAGAAGA ACAAGTTGCG AGTTTACTAC
    TTGTCCTGGT TGAGAAATAA AATCCTTCAC AATGATCCCG AAGTAGAAAA GAAACAGGGT
    TGGACAACGG TGGGTGACTT GGAGGGCTGT GTGCACTATA AAGTTGTAAA ATATGAGAGA
    ATCAAATTCT TGGTAATTGC GTTGAAGAGT TCTGTGGAAG TCTATGCATG GGCGCCAAAG
    CCATACCACA AATTTATGGC CTTTAAGTCA TTTGGTGAAT TGGTACATAA GCCATTGCTG
    GTGGATCTAA CTGTAGAAGA GGGTCAGAGA CTAAAAGTCA TTTATGGCTC CTGTGCTGGC
    TTCCATGCTG TGGACGTGGA TTCGGGATCA GTCTACGATA TTTATCTACC AACACATATC
    CAGAGCAGTA TCCAACCTCA CGCAATCATA ATTCTCCCAA ATACGGATGG AATGGAGCTT
    CTGGTCTGTT ATGAGGATGA AGGAGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT
    GTGGTTCTGC AGTGGGGAGA AATGCCAACT TCAGTTGCAT ATATTCGCTC CAATCAGATT
    ATGGGCTGGG GAGAAAAAGC GATCGAAATA CGCTCGGTAG AAACTGGACA CTTGGACGGT
    GTGTTCATGC ACAAAAGGGC ACAAAGACTC AAGTTCTTAT GTGAACGCAA TGACAAGGTT
    TTCTTTGCCT CCGTACGGTC GGGTGGAAGC AGTCAAGTTT ATTTCATGAC CCTTGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30641
    Clone ID Related Accession (Same CDS sequence) XM_015277808.1
    Accession Version XM_015277808.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763584.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACCTGT GAGAACAACA TCCCGATCAC CAGTCCTGTC CCGTCGTGAT
    TCTCCACTGC AGGGCAGTGG GCAGCAAAAT AACCAAGCAG GTCAAAGAAA CTCCACGAGC
    AATATAGAGC CTCGTCTGCT GTGGGAAAGG GTGGAGAAGC TTGTACCAAG ACCAGGCAGT
    GGCAGTTCTT CAGGTTCAAG CAACTCCGGC TCACAGCCTG GTTCCCACCC TGGCTCTCAG
    AGTGGGTCTG GAGAGCGGTT CCGGATGAGA TCATCATCCA AATCAGAGGG TTCACCATCG
    CAGCGTCATG AAAGTGCACC TAAAAAGCCT GAAGAGAAAA AGGAGGTCTT CAGACCTATC
    AAGCCTGCTG GAGAAGTGGA TTTAACTGCA TTGGCAAAGG AGCTGCGAGC AGTAGAGGAT
    GTGCGACCTC CACATAAAGT GACGGATTAT TCGTCCTCAA GTGAGGAGTC AGGGACGACA
    GATGAAGAAG ATGATGATAT GGAACAAGAA GGAGCAGATG AATCTACTTC TGGACCAGAT
    GATGTCCGAG CAGTGTCAAC ACTGAACTTG AGCAATGGTG AAACAGAGTC TGTAAAAACT
    ATGATTGTCC ATGATGATGT GGAGAGTGAA CCTGCCTTGA CTCCTTCTAA GGAGGGCACC
    TTAATTGTCC GACAGACTCA GTCCGCTAGT AACACACTCC AGAAACACAA ATCTTCCTCC
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG TGGGACAACA
    GTGACTTCTG TGGTAGGATT TTCTAGTGAA GCAATGAGAA CAGAGGCAAT AAGGCAAGAC
    CCTACACGGA AAGGATCAGT TGTCAACGTG AATCCTACAA ACACAAGACC ACAGAGCGAT
    ACCCCAGAGA TCCGCAAATA CAAGAAAAGA TTCAATTCTG AGATACTGTG TGCTGCCTTA
    TGGGGAGTGA ACTTGTTAGT GGGCACAGAA AGTGGTCTGA TGCTGCTAGA CAGAAGTGGT
    CAGGGGAAGG TTTATCCGCT CATCAATCGA AGAAGATTCC AGCAAATGGA TGTACTTGAA
    GGGCTAAATG TCTTGGTGAC AATTTCTGGT AAGAAGAACA AGTTGCGAGT TTACTACTTG
    TCCTGGTTGA GAAATAAAAT CCTTCACAAT GATCCCGAAG TAGAAAAGAA ACAGGGTTGG
    ACAACGGTGG GTGACTTGGA GGGCTGTGTG CACTATAAAG TTGTAAAATA TGAGAGAATC
    AAATTCTTGG TAATTGCGTT GAAGAGTTCT GTGGAAGTCT ATGCATGGGC GCCAAAGCCA
    TACCACAAAT TTATGGCCTT TAAGTCATTT GGTGAATTGG TACATAAGCC ATTGCTGGTG
    GATCTAACTG TAGAAGAGGG TCAGAGACTA AAAGTCATTT ATGGCTCCTG TGCTGGCTTC
    CATGCTGTGG ACGTGGATTC GGGATCAGTC TACGATATTT ATCTACCAAC ACATATCCAG
    AGCAGTATCC AACCTCACGC AATCATAATT CTCCCAAATA CGGATGGAAT GGAGCTTCTG
    GTCTGTTATG AGGATGAAGG AGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTG
    GTTCTGCAGT GGGGAGAAAT GCCAACTTCA GTTGCATATA TTCGCTCCAA TCAGATTATG
    GGCTGGGGAG AAAAAGCGAT CGAAATACGC TCGGTAGAAA CTGGACACTT GGACGGTGTG
    TTCATGCACA AAAGGGCACA AAGACTCAAG TTCTTATGTG AACGCAATGA CAAGGTTTTC
    TTTGCCTCCG TACGGTCGGG TGGAAGCAGT CAAGTTTATT TCATGACCCT TGGCAGGACT
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133294.1
    CDS33..3770
    Translation

    Target ORF information:

    RefSeq Version XM_015277808.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277808.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACCTGT GAGAACAACA TCCCGATCAC CAGTCCTGTC CCGTCGTGAT
    TCTCCACTGC AGGGCAGTGG GCAGCAAAAT AACCAAGCAG GTCAAAGAAA CTCCACGAGC
    AATATAGAGC CTCGTCTGCT GTGGGAAAGG GTGGAGAAGC TTGTACCAAG ACCAGGCAGT
    GGCAGTTCTT CAGGTTCAAG CAACTCCGGC TCACAGCCTG GTTCCCACCC TGGCTCTCAG
    AGTGGGTCTG GAGAGCGGTT CCGGATGAGA TCATCATCCA AATCAGAGGG TTCACCATCG
    CAGCGTCATG AAAGTGCACC TAAAAAGCCT GAAGAGAAAA AGGAGGTCTT CAGACCTATC
    AAGCCTGCTG GAGAAGTGGA TTTAACTGCA TTGGCAAAGG AGCTGCGAGC AGTAGAGGAT
    GTGCGACCTC CACATAAAGT GACGGATTAT TCGTCCTCAA GTGAGGAGTC AGGGACGACA
    GATGAAGAAG ATGATGATAT GGAACAAGAA GGAGCAGATG AATCTACTTC TGGACCAGAT
    GATGTCCGAG CAGTGTCAAC ACTGAACTTG AGCAATGGTG AAACAGAGTC TGTAAAAACT
    ATGATTGTCC ATGATGATGT GGAGAGTGAA CCTGCCTTGA CTCCTTCTAA GGAGGGCACC
    TTAATTGTCC GACAGACTCA GTCCGCTAGT AACACACTCC AGAAACACAA ATCTTCCTCC
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG TGGGACAACA
    GTGACTTCTG TGGTAGGATT TTCTAGTGAA GCAATGAGAA CAGAGGCAAT AAGGCAAGAC
    CCTACACGGA AAGGATCAGT TGTCAACGTG AATCCTACAA ACACAAGACC ACAGAGCGAT
    ACCCCAGAGA TCCGCAAATA CAAGAAAAGA TTCAATTCTG AGATACTGTG TGCTGCCTTA
    TGGGGAGTGA ACTTGTTAGT GGGCACAGAA AGTGGTCTGA TGCTGCTAGA CAGAAGTGGT
    CAGGGGAAGG TTTATCCGCT CATCAATCGA AGAAGATTCC AGCAAATGGA TGTACTTGAA
    GGGCTAAATG TCTTGGTGAC AATTTCTGGT AAGAAGAACA AGTTGCGAGT TTACTACTTG
    TCCTGGTTGA GAAATAAAAT CCTTCACAAT GATCCCGAAG TAGAAAAGAA ACAGGGTTGG
    ACAACGGTGG GTGACTTGGA GGGCTGTGTG CACTATAAAG TTGTAAAATA TGAGAGAATC
    AAATTCTTGG TAATTGCGTT GAAGAGTTCT GTGGAAGTCT ATGCATGGGC GCCAAAGCCA
    TACCACAAAT TTATGGCCTT TAAGTCATTT GGTGAATTGG TACATAAGCC ATTGCTGGTG
    GATCTAACTG TAGAAGAGGG TCAGAGACTA AAAGTCATTT ATGGCTCCTG TGCTGGCTTC
    CATGCTGTGG ACGTGGATTC GGGATCAGTC TACGATATTT ATCTACCAAC ACATATCCAG
    AGCAGTATCC AACCTCACGC AATCATAATT CTCCCAAATA CGGATGGAAT GGAGCTTCTG
    GTCTGTTATG AGGATGAAGG AGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTG
    GTTCTGCAGT GGGGAGAAAT GCCAACTTCA GTTGCATATA TTCGCTCCAA TCAGATTATG
    GGCTGGGGAG AAAAAGCGAT CGAAATACGC TCGGTAGAAA CTGGACACTT GGACGGTGTG
    TTCATGCACA AAAGGGCACA AAGACTCAAG TTCTTATGTG AACGCAATGA CAAGGTTTTC
    TTTGCCTCCG TACGGTCGGG TGGAAGCAGT CAAGTTTATT TCATGACCCT TGGCAGGACT
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30642
    Clone ID Related Accession (Same CDS sequence) XM_015277809.1
    Accession Version XM_015277809.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763584.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACCTGT GAGAACAACA TCCCGATCAC CAGTCCTGTC CCGTCGTGAT
    TCTCCACTGC AGGGCAGTGG GCAGCAAAAT AACCAAGCAG GTCAAAGAAA CTCCACGAGC
    AATATAGAGC CTCGTCTGCT GTGGGAAAGG GTGGAGAAGC TTGTACCAAG ACCAGGCAGT
    GGCAGTTCTT CAGGTTCAAG CAACTCCGGC TCACAGCCTG GTTCCCACCC TGGCTCTCAG
    AGTGGGTCTG GAGAGCGGTT CCGGATGAGA TCATCATCCA AATCAGAGGG TTCACCATCG
    CAGCGTCATG AAAGTGCACC TAAAAAGCCT GAAGAGAAAA AGGAGGTCTT CAGACCTATC
    AAGCCTGCTG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGGACAAC AGTGACTTCT
    GTGGTAGGAT TTTCTAGTGA AGCAATGAGA ACAGAGGCAA TAAGGCAAGA CCCTACACGG
    AAAGGATCAG TTGTCAACGT GAATCCTACA AACACAAGAC CACAGAGCGA TACCCCAGAG
    ATCCGCAAAT ACAAGAAAAG ATTCAATTCT GAGATACTGT GTGCTGCCTT ATGGGGAGTG
    AACTTGTTAG TGGGCACAGA AAGTGGTCTG ATGCTGCTAG ACAGAAGTGG TCAGGGGAAG
    GTTTATCCGC TCATCAATCG AAGAAGATTC CAGCAAATGG ATGTACTTGA AGGGCTAAAT
    GTCTTGGTGA CAATTTCTGG TAAGAAGAAC AAGTTGCGAG TTTACTACTT GTCCTGGTTG
    AGAAATAAAA TCCTTCACAA TGATCCCGAA GTAGAAAAGA AACAGGGTTG GACAACGGTG
    GGTGACTTGG AGGGCTGTGT GCACTATAAA GTTGTAAAAT ATGAGAGAAT CAAATTCTTG
    GTAATTGCGT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CGCCAAAGCC ATACCACAAA
    TTTATGGCCT TTAAGTCATT TGGTGAATTG GTACATAAGC CATTGCTGGT GGATCTAACT
    GTAGAAGAGG GTCAGAGACT AAAAGTCATT TATGGCTCCT GTGCTGGCTT CCATGCTGTG
    GACGTGGATT CGGGATCAGT CTACGATATT TATCTACCAA CACATATCCA GAGCAGTATC
    CAACCTCACG CAATCATAAT TCTCCCAAAT ACGGATGGAA TGGAGCTTCT GGTCTGTTAT
    GAGGATGAAG GAGTTTATGT AAACACATAT GGAAGGATCA CCAAGGATGT GGTTCTGCAG
    TGGGGAGAAA TGCCAACTTC AGTTGCATAT ATTCGCTCCA ATCAGATTAT GGGCTGGGGA
    GAAAAAGCGA TCGAAATACG CTCGGTAGAA ACTGGACACT TGGACGGTGT GTTCATGCAC
    AAAAGGGCAC AAAGACTCAA GTTCTTATGT GAACGCAATG ACAAGGTTTT CTTTGCCTCC
    GTACGGTCGG GTGGAAGCAG TCAAGTTTAT TTCATGACCC TTGGCAGGAC TTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133295.1
    CDS33..3761
    Translation

    Target ORF information:

    RefSeq Version XM_015277809.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277809.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ATTCTCCTGC AAAAAGTCTG GTGGACATCG ACCTTTCCTC CCTGCGGGAC 
    CCCGCCGGGA TTTTTGAGCT GGTGGAAGTG GTTGGCAATG GCACGTACGG GCAAGTCTAC
    AAGGGTCGAC ATGTCAAAAC GGGCCAGTTG GCAGCCATCA AAGTCATGGA CGTTACTGAG
    GATGAAGAAG AAGAAATCAA ACTGGAGATT AATATGCTAA AGAAATATTC TCATCATAGA
    AATATTGCAA CTTATTATGG TGCTTTCATT AAGAAAAGTC CTCCAGGACA TGATGACCAA
    CTGTGGCTTG TTATGGAGTT TTGTGGAGCA GGCTCTATCA CGGACCTCGT GAAGAACACG
    AAAGGGAATA CTCTGAAAGA AGACTGGATA GCATACATCT CCAGAGAGAT TCTCAGGGGG
    CTGGCACATC TTCATGCCCA CCATGTGATT CACAGGGATA TCAAAGGGCA AAATGTGTTG
    TTAACAGAGA ATGCAGAAGT GAAACTCGTG GATTTTGGTG TGAGCGCTCA GCTGGACAGG
    ACAGTTGGCA GGAGAAACAC TTTCATTGGA ACTCCATACT GGATGGCTCC AGAAGTTATT
    GCCTGTGATG AAAATCCAGA TGCTACGTAT GATTACAGAA GTGACCTCTG GTCCTGCGGC
    ATTACAGCCA TAGAAATGGC AGAAGGAGCT CCCCCACTTT GTGACATGCA TCCCATGAGG
    GCACTCTTTC TGATACCCAG AAACCCTCCC CCACGGTTAA AATCCAAGAA ATGGTCGAAA
    AAGTTTTTCA GCTTTATAGA AGGTTGTCTA GTGAAGAATT ACATGCAGAG ACCTTCTACA
    GAGCAACTAC TGAAACATCC ATTTATACGT GACCAACCAA ATGAAAGGCA AGTCCGAATC
    CAGCTTAAGG ACCATATAGA CAGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAGACTGAG
    TATGAATATA GTGGAAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAAGA AGGAGAGCCA
    AGTTCCATTG TCAATGTGCC TGGAGAATCA ACCCTCCGAC GTGATTTCCT GAGGCTGCAG
    CAGGAAAACA AGGAACGATC CGAGGCCCTT CGGAGACAGC AGCTGCTGCA GGAGCAGCAG
    CTTCGTGAGC AGGAGGAGTA CAAGAGGCAG CTACTGGCAG AGAGACAGAA GCGCATTGAG
    CAGCAGAAGG AGCAAAGGAG ACGGCTGGAA GAGCAACAAA GAAGAGAACG GGAAGCTCGC
    AGGCAACAAG AGCGTGAACA AAGGCGGAGG GAACAGGAAG AAAAAAGACG TCTGGAAGAA
    ATGGAGAGGA GACGTAAGGA AGAGGAAGAG AGGAGAAGAG CAGAGGAGGA AAAGAGGAGG
    GTAGAAAGGG AGCAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    ATTCTACAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAATACAG ATGGCGAGAG
    ATGGAGGAGC ATCGACAGGC AGAGAGGCTC CAGCGCCAGT TGCAGCAGGA GCAAGCCTAC
    CTCCTGTCAC TGCAGCATGA TCACAGGCGG CAGCAGCAGC AGCAACAGCA GCAGCAGCAG
    CAACAGCAGC AGCAGCAAGA AAGAAATAAA CAAAGCTATC ATACCCCTGA GCCTAAATCC
    CACTATGAGC CTGCTGAAAG AGCGCGTGAG GTGGAGGAGA GATTTAGAAA AACGAATCAG
    GGCTCCCCTG AAGCACAGTC TAAGCAGATG GGCAAAGTGT TGGAGCCACC AGTGCCTTCA
    AGATCAGAGT CCTTTTCCAA TGGAAACTCA GAACCTGCTC AGCCTGCCCT GCAGAGGCCA
    ATGGAGCCCC AAGTACCTGT GAGAACAACA TCCCGATCAC CAGTCCTGTC CCGTCGTGAT
    TCTCCACTGC AGGGCAGTGG GCAGCAAAAT AACCAAGCAG GTCAAAGAAA CTCCACGAGC
    AATATAGAGC CTCGTCTGCT GTGGGAAAGG GTGGAGAAGC TTGTACCAAG ACCAGGCAGT
    GGCAGTTCTT CAGGTTCAAG CAACTCCGGC TCACAGCCTG GTTCCCACCC TGGCTCTCAG
    AGTGGGTCTG GAGAGCGGTT CCGGATGAGA TCATCATCCA AATCAGAGGG TTCACCATCG
    CAGCGTCATG AAAGTGCACC TAAAAAGCCT GAAGAGAAAA AGGAGGTCTT CAGACCTATC
    AAGCCTGCTG ATTTAACTGC ATTGGCAAAG GAGCTGCGAG CAGTAGAGGA TGTGCGACCT
    CCACATAAAG TGACGGATTA TTCGTCCTCA AGTGAGGAGT CAGGGACGAC AGATGAAGAA
    GATGATGATA TGGAACAAGA AGGAGCAGAT GAATCTACTT CTGGACCAGA TGATGTCCGA
    GCAGTGTCAA CACTGAACTT GAGCAATGGT GAAACAGAGT CTGTAAAAAC TATGATTGTC
    CATGATGATG TGGAGAGTGA ACCTGCCTTG ACTCCTTCTA AGGAGGGCAC CTTAATTGTC
    CGACAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GTGGGACAAC AGTGACTTCT
    GTGGTAGGAT TTTCTAGTGA AGCAATGAGA ACAGAGGCAA TAAGGCAAGA CCCTACACGG
    AAAGGATCAG TTGTCAACGT GAATCCTACA AACACAAGAC CACAGAGCGA TACCCCAGAG
    ATCCGCAAAT ACAAGAAAAG ATTCAATTCT GAGATACTGT GTGCTGCCTT ATGGGGAGTG
    AACTTGTTAG TGGGCACAGA AAGTGGTCTG ATGCTGCTAG ACAGAAGTGG TCAGGGGAAG
    GTTTATCCGC TCATCAATCG AAGAAGATTC CAGCAAATGG ATGTACTTGA AGGGCTAAAT
    GTCTTGGTGA CAATTTCTGG TAAGAAGAAC AAGTTGCGAG TTTACTACTT GTCCTGGTTG
    AGAAATAAAA TCCTTCACAA TGATCCCGAA GTAGAAAAGA AACAGGGTTG GACAACGGTG
    GGTGACTTGG AGGGCTGTGT GCACTATAAA GTTGTAAAAT ATGAGAGAAT CAAATTCTTG
    GTAATTGCGT TGAAGAGTTC TGTGGAAGTC TATGCATGGG CGCCAAAGCC ATACCACAAA
    TTTATGGCCT TTAAGTCATT TGGTGAATTG GTACATAAGC CATTGCTGGT GGATCTAACT
    GTAGAAGAGG GTCAGAGACT AAAAGTCATT TATGGCTCCT GTGCTGGCTT CCATGCTGTG
    GACGTGGATT CGGGATCAGT CTACGATATT TATCTACCAA CACATATCCA GAGCAGTATC
    CAACCTCACG CAATCATAAT TCTCCCAAAT ACGGATGGAA TGGAGCTTCT GGTCTGTTAT
    GAGGATGAAG GAGTTTATGT AAACACATAT GGAAGGATCA CCAAGGATGT GGTTCTGCAG
    TGGGGAGAAA TGCCAACTTC AGTTGCATAT ATTCGCTCCA ATCAGATTAT GGGCTGGGGA
    GAAAAAGCGA TCGAAATACG CTCGGTAGAA ACTGGACACT TGGACGGTGT GTTCATGCAC
    AAAAGGGCAC AAAGACTCAA GTTCTTATGT GAACGCAATG ACAAGGTTTT CTTTGCCTCC
    GTACGGTCGG GTGGAAGCAG TCAAGTTTAT TTCATGACCC TTGGCAGGAC TTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.