MAP4K4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP4K4
Entrez Gene ID	9448
Full Name	mitogen-activated protein kinase kinase kinase kinase 4
Synonyms	FLH21957,HEL-S-31,HGK,MEKKK4,NIK
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase kinase 4 Names mitogen-activated protein kinase kinase kinase kinase 4 HPK/GCK-like kinase HGK MAPK/ERK kinase kinase kinase 4 MEK kinase kinase 4 Ste20 group protein kinase HGK epididymis secretory protein Li 31 hepatocyte progenitor kinase-like/germinal center kinase-like kinase nck-interacting kinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q11.2
Summary	The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].
Disorder MIM:	604666

mRNA and Protein(s)

mRNA	Protein	Name
NM_145687.3	NP_663720.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform 3
XM_005264048.2	XP_005264105.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
XM_017005356.1	XP_016860845.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X15
XM_017005363.1	XP_016860852.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X26
XM_017005367.1	XP_016860856.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X30
XM_005264065.3	XP_005264122.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X31
XM_005264066.3	XP_005264123.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X32
XM_005264069.2	XP_005264126.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X35
XM_017005375.1	XP_016860864.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X44
XM_006712868.2	XP_006712931.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X45
NM_004834.4	NP_004825.3	mitogen-activated protein kinase kinase kinase kinase 4 isoform 1
XM_017005349.1	XP_016860838.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
XM_017005355.1	XP_016860844.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X13
XM_017005370.1	XP_016860859.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X38
XM_005264071.2	XP_005264128.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X40
XM_017005372.1	XP_016860861.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X41
XM_017005352.1	XP_016860841.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
XM_005264060.3	XP_005264117.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X16
XM_017005357.1	XP_016860846.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X17
XM_017005361.1	XP_016860850.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X21
XM_017005362.1	XP_016860851.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X22
XM_005264062.3	XP_005264119.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X23
XM_017005364.1	XP_016860853.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X27
NM_145687.4	NP_663720.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform 3
NM_001242560.2	NP_001229489.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform 5
NM_004834.5	NP_004825.3	mitogen-activated protein kinase kinase kinase kinase 4 isoform 1
NM_001242560.1	NP_001229489.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform 5
NM_145686.3	NP_663719.2	mitogen-activated protein kinase kinase kinase kinase 4 isoform 2
XM_005264052.3	XP_005264109.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
XM_005264058.3	XP_005264115.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
XM_017005351.1	XP_016860840.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
XM_006712865.2	XP_006712928.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
XM_017005353.1	XP_016860842.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
XM_005264059.3	XP_005264116.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X14
XM_005264063.3	XP_005264120.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X24
XM_005264064.3	XP_005264121.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X25
XM_017005365.1	XP_016860854.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X28
XM_006712869.2	XP_006712932.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X37
XM_017005371.1	XP_016860860.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X39
NM_001242559.1	NP_001229488.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform 4
NM_001242559.2	NP_001229488.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform 4
XM_005264050.3	XP_005264107.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
XM_017005350.1	XP_016860839.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
XM_005264057.3	XP_005264114.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
XM_017005354.1	XP_016860843.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X12
XM_017005358.1	XP_016860847.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X18
XM_017005359.1	XP_016860848.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X19
XM_017005360.1	XP_016860849.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X20
XM_017005366.1	XP_016860855.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X29
XM_005264068.3	XP_005264125.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X33
XM_017005368.1	XP_016860857.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X34
XM_017005369.1	XP_016860858.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X36
XM_017005373.1	XP_016860862.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X42
XM_017005374.1	XP_016860863.1	mitogen-activated protein kinase kinase kinase kinase 4 isoform X43

Pathways from BioSystems

Homologies

	MAP4K4	XP_001107420.2
Bos taurus (cattle)	MAP4K4	XP_003586662.2
Canis lupus familiaris (dog)	MAP4K4	XP_005626291.1
Homo sapiens (human)	MAP4K4	NP_663719.2
Rattus norvegicus (Norway rat)	Map4k4	NP_001100374.1
Xenopus tropicalis (tropical clawed frog)	map4k4	XP_002934552.1
Pan troglodytes (chimpanzee)	MAP4K4	XP_515665.3
Mus musculus (house mouse)	Map4k4	NP_001239129.1
Gallus gallus (chicken)	MAP4K4	XP_416913.4
Danio rerio (zebrafish)	wu:fa03e11	XP_001343426.5

Gene Ontology
Bibliography

Related articles in PubMed

RBM4-SRSF3-MAP4K4 splicing cascade modulates the metastatic signature of colorectal cancer cell.
Lin JC, Lee YC, Tan TH, Liang YC, Chuang HC, Fann YC, Johnson KR, Lin YJ
Biochimica et biophysica acta1865(2)259-272(2018 Feb)

The Ser/Thr kinase MAP4K4 drives c-Met-induced motility and invasiveness in a cell-based model of SHH medulloblastoma.
Santhana Kumar K, Tripolitsioti D, Ma M, Gr?hlert J, Egli KB, Fiaschetti G, Shalaby T, Grotzer MA, Baumgartner M
SpringerPlus419(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Interactions between MAP4K4 gene variants and environmental factors may contribute to MAP4K4 attenuation in T cells, leading to non-obese T2D.
Title: MAP4K4 and IL-6

MAP4K4 promotes the epithelial-mesenchymal transition and invasiveness of hepatocellular carcinoma cells
Title: MAP4K4 promotes epithelial-mesenchymal transition and metastasis in hepatocellular carcinoma.

Results reveal that endothelial Map4k4 is critical for lymphatic vascular development by regulating endothelial cells (EC)quiescence and lymphatic EC fate.
Title: Endothelial Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4 Is Critical for Lymphatic Vascular Development and Function.

of the five variants, SNP rs2236935T/C was significantly associated with type 2 diabetes mellitus (T2DM) in this study population; conclude that MAP4K4 gene is associated with T2DM in a Chinese Han population, and MAP4K4 gene variants may contribute to the risk toward the development of T2DM
Title: Association of Common Genetic Variants in Mitogen-activated Protein Kinase Kinase Kinase Kinase 4 with Type 2 Diabetes Mellitus in a Chinese Han Population.

This report identifies HGK methylation/downregulation in T cells as a potential biomarker for non-obese type 2 diabetes
Title: Epigenetic regulation of HGK/MAP4K4 in T cells of type 2 diabetes patients.

The following MAP4K4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP4K4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu92772	XM_017005349.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu92773	XM_017005350.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92774	XM_017005351.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92775	XM_017005352.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92776	XM_017005353.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92777	XM_017005354.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92778	XM_017005355.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92779	XM_017005356.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92780	XM_017005357.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92781	XM_017005358.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92782	XM_017005359.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92783	XM_017005360.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92784	XM_017005361.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92785	XM_017005362.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92786	XM_017005363.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92787	XM_017005364.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92788	XM_017005365.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92789	XM_017005366.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92790	XM_017005367.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92791	XM_017005368.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92792	XM_017005369.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92793	XM_017005370.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92794	XM_017005371.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92795	XM_017005372.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X41, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu92796	XM_017005373.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X42, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu92797	XM_017005374.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X43, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu92798	XM_017005375.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X44, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu20375	NM_145687.4 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$853.30 $1219.00
OHu20734	NM_145686.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$923.30 $1319.00
OHu21057	NM_004834.5 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$825.30 $1179.00
OHu21579	NM_001242560.2 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu22392	NM_001242559.2 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36834	XM_005264057.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36835	XM_005264058.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36836	XM_006712865.2 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36838	XM_005264059.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36839	XM_005264060.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36841	XM_005264062.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36842	XM_005264063.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36843	XM_005264064.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36844	XM_005264065.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36845	XM_005264066.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36846	XM_005264068.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36847	XM_005264069.2 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36848	XM_005264071.2 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X40, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36850	XM_006712868.2 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X45, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36851	XM_006712869.2 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu36825	XM_005264048.2 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu36828	XM_005264050.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu36829	XM_005264052.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu20375	NM_145687.3	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$853.30 $1219.00
OHu21057	NM_004834.4	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$825.30 $1179.00
OHu21579	NM_001242560.1	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu22392	NM_001242559.1	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP4K4 ( XM_017005349.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860838.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.

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CloneID	OHu92772
Clone ID Related Accession (Same CDS sequence)	XM_017005349.1
Accession Version	XM_017005349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
AAGAACAATG TTTCCCCTGT CTCGCGATCC CATTCCTTCA GTGACCCTTC TCCCAAATTT 
GCACACCACC ATCTTCGTTC TCAGGACCCA TGTCCACCTT CCCGCAGTGA GGTGCTCAGT 
CAGAGCTCTG ACTCTAAGTC AGAGGCGCCT GACCCTACCC AAAAGGCTTG GTCTAGATCA 
GACAGTGACG AGGTGCCTCC AAGGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA 
CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT 
GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC 
TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC 
AGACCCCTCA AGCCTGCTGG CGAAGTGGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA 
GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG 
GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA 
GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT 
GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG 
GAGGGCACTC TAATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG 
AGCAATGGCT TTGCCGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC 
TCCTCCACTT CCTCCACCTC CTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC 
CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC 
CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA 
CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC 
AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT 
GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG 
ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT 
CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT 
AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA 
GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA 
GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC 
TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG 
GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC 
TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT 
TATCTACCAA CACATATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT 
ACAGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT 
GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACATC AGTAGCATAT 
ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA 
ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT 
GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT 
TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860838.1
CDS	75..4133
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005349.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005349.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
AAGAACAATG TTTCCCCTGT CTCGCGATCC CATTCCTTCA GTGACCCTTC TCCCAAATTT 
GCACACCACC ATCTTCGTTC TCAGGACCCA TGTCCACCTT CCCGCAGTGA GGTGCTCAGT 
CAGAGCTCTG ACTCTAAGTC AGAGGCGCCT GACCCTACCC AAAAGGCTTG GTCTAGATCA 
GACAGTGACG AGGTGCCTCC AAGGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA 
CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT 
GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC 
TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC 
AGACCCCTCA AGCCTGCTGG CGAAGTGGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA 
GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG 
GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA 
GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT 
GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG 
GAGGGCACTC TAATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG 
AGCAATGGCT TTGCCGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC 
TCCTCCACTT CCTCCACCTC CTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC 
CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC 
CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA 
CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC 
AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT 
GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG 
ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT 
CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT 
AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA 
GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA 
GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC 
TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG 
GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC 
TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT 
TATCTACCAA CACATATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT 
ACAGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT 
GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACATC AGTAGCATAT 
ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA 
ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT 
GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT 
TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005350.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860839.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.

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CloneID	OHu92773
Clone ID Related Accession (Same CDS sequence)	XM_017005350.1
Accession Version	XM_017005350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC 
CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT 
GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC 
GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA 
GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC 
AGCAGTATTG AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC 
AGTGGCAGCT CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT 
CAGAGTGGCT CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA 
TCTCAGCGCC TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC 
CTCAAGCCTG CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA 
GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG 
ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA 
GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA 
ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC 
ACTCTAATCG TCCGCCAGAG TACAGTTGAC CAAAAGCGTG CCAGCCATCA TGAGAGCAAT 
GGCTTTGCCG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC 
ACTTCCTCCA CCTCCTCCTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG 
ACACCCCAGG ACAAGCTCAC TGCTAATGAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA 
CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
TCTAGCGGAA CAACAGTGAC ATCTGTGGTG GGATTTTCCT GTGATGGGAT GAGACCAGAA 
GCCATAAGGC AAGATCCTAC CCGGAAAGGC TCAGTGGTCA ATGTGAATCC TACCAACACT 
AGGCCACAGA GTGACACCCC GGAGATTCGT AAATACAAGA AGAGGTTTAA CTCTGAGATT 
CTGTGTGCTG CCTTATGGGG AGTGAATTTG CTAGTGGGTA CAGAGAGTGG CCTGATGCTG 
CTGGACAGAA GTGGCCAAGG GAAGGTCTAT CCTCTTATCA ACCGAAGACG ATTTCAACAA 
ATGGACGTAC TTGAGGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA 
CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAATGATCC AGAAGTTGAG 
AAGAAGCAGG GATGGACAAC CGTAGGGGAT TTGGAAGGAT GTGTACATTA TAAAGTTGTA 
AAATATGAAA GAATCAAATT TCTGGTGATT GCTTTGAAGA GTTCTGTGGA AGTCTATGCG 
TGGGCACCAA AGCCATATCA CAAATTTATG GCCTTTAAGT CATTTGGAGA ATTGGTACAT 
AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA 
TCCTGTGCTG GATTCCATGC TGTTGATGTG GATTCAGGAT CAGTCTATGA CATTTATCTA 
CCAACACATA TCCAGTGTAG CATCAAACCC CATGCAATCA TCATCCTCCC CAATACAGAT 
GGAATGGAGC TTCTGGTGTG CTATGAAGAT GAGGGGGTTT ATGTAAACAC ATATGGAAGG 
ATCACCAAGG ATGTAGTTCT ACAGTGGGGA GAGATGCCTA CATCAGTAGC ATATATTCGA 
TCCAATCAGA CAATGGGCTG GGGAGAGAAG GCCATAGAGA TCCGATCTGT GGAAACTGGT 
CACTTGGATG GTGTGTTCAT GCACAAAAGG GCTCAAAGAC TAAAATTCTT GTGTGAACGC 
AATGACAAGG TGTTCTTTGC CTCTGTTCGG TCTGGTGGCA GCAGTCAGGT TTATTTCATG 
ACCTTAGGCA GGACTTCTCT TCTGAGCTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860839.1
CDS	75..4067
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005350.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005350.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC 
CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT 
GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC 
GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA 
GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC 
AGCAGTATTG AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC 
AGTGGCAGCT CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT 
CAGAGTGGCT CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA 
TCTCAGCGCC TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC 
CTCAAGCCTG CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA 
GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG 
ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA 
GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA 
ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC 
ACTCTAATCG TCCGCCAGAG TACAGTTGAC CAAAAGCGTG CCAGCCATCA TGAGAGCAAT 
GGCTTTGCCG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC 
ACTTCCTCCA CCTCCTCCTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG 
ACACCCCAGG ACAAGCTCAC TGCTAATGAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA 
CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
TCTAGCGGAA CAACAGTGAC ATCTGTGGTG GGATTTTCCT GTGATGGGAT GAGACCAGAA 
GCCATAAGGC AAGATCCTAC CCGGAAAGGC TCAGTGGTCA ATGTGAATCC TACCAACACT 
AGGCCACAGA GTGACACCCC GGAGATTCGT AAATACAAGA AGAGGTTTAA CTCTGAGATT 
CTGTGTGCTG CCTTATGGGG AGTGAATTTG CTAGTGGGTA CAGAGAGTGG CCTGATGCTG 
CTGGACAGAA GTGGCCAAGG GAAGGTCTAT CCTCTTATCA ACCGAAGACG ATTTCAACAA 
ATGGACGTAC TTGAGGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA 
CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAATGATCC AGAAGTTGAG 
AAGAAGCAGG GATGGACAAC CGTAGGGGAT TTGGAAGGAT GTGTACATTA TAAAGTTGTA 
AAATATGAAA GAATCAAATT TCTGGTGATT GCTTTGAAGA GTTCTGTGGA AGTCTATGCG 
TGGGCACCAA AGCCATATCA CAAATTTATG GCCTTTAAGT CATTTGGAGA ATTGGTACAT 
AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA 
TCCTGTGCTG GATTCCATGC TGTTGATGTG GATTCAGGAT CAGTCTATGA CATTTATCTA 
CCAACACATA TCCAGTGTAG CATCAAACCC CATGCAATCA TCATCCTCCC CAATACAGAT 
GGAATGGAGC TTCTGGTGTG CTATGAAGAT GAGGGGGTTT ATGTAAACAC ATATGGAAGG 
ATCACCAAGG ATGTAGTTCT ACAGTGGGGA GAGATGCCTA CATCAGTAGC ATATATTCGA 
TCCAATCAGA CAATGGGCTG GGGAGAGAAG GCCATAGAGA TCCGATCTGT GGAAACTGGT 
CACTTGGATG GTGTGTTCAT GCACAAAAGG GCTCAAAGAC TAAAATTCTT GTGTGAACGC 
AATGACAAGG TGTTCTTTGC CTCTGTTCGG TCTGGTGGCA GCAGTCAGGT TTATTTCATG 
ACCTTAGGCA GGACTTCTCT TCTGAGCTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005351.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860840.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.

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CloneID	OHu92774
Clone ID Related Accession (Same CDS sequence)	XM_017005351.1
Accession Version	XM_017005351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG 
CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC 
TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC 
GGGGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG 
GAAAATGCAG TGAAAAAACC TGAAGATAAA AAGGAAGTTT TCAGACCCCT CAAGCCTGCT 
GATCTGACCG CACTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTACGGCC ACCTCACAAA 
GTAACGGACT ACTCCTCATC CAGTGAGGAG TCGGGGACGA CGGATGAGGA GGACGACGAT 
GTGGAGCAGG AAGGGGCTGA CGAGTCCACC TCAGGACCAG AGGACACCAG AGCAGCGTCA 
TCTCTGAATT TGAGCAATGG TGAAACGGAA TCTGTGAAAA CCATGATTGT CCATGATGAT 
GTAGAAAGTG AGCCGGCCAT GACCCCATCC AAGGAGGGCA CTCTAATCGT CCGCCAGAGT 
ACAGTTGACC AAAAGCGTGC CAGCCATCAT GAGAGCAATG GCTTTGCCGG TCGCATTCAC 
CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC 
CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTCACT 
GCTAATGAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGCGGAAC AACAGTGACA 
TCTGTGGTGG GATTTTCCTG TGATGGGATG AGACCAGAAG CCATAAGGCA AGATCCTACC 
CGGAAAGGCT CAGTGGTCAA TGTGAATCCT ACCAACACTA GGCCACAGAG TGACACCCCG 
GAGATTCGTA AATACAAGAA GAGGTTTAAC TCTGAGATTC TGTGTGCTGC CTTATGGGGA 
GTGAATTTGC TAGTGGGTAC AGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG 
AAGGTCTATC CTCTTATCAA CCGAAGACGA TTTCAACAAA TGGACGTACT TGAGGGCTTG 
AATGTCTTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG 
TTAAGAAATA AAATACTTCA CAATGATCCA GAAGTTGAGA AGAAGCAGGG ATGGACAACC 
GTAGGGGATT TGGAAGGATG TGTACATTAT AAAGTTGTAA AATATGAAAG AATCAAATTT 
CTGGTGATTG CTTTGAAGAG TTCTGTGGAA GTCTATGCGT GGGCACCAAA GCCATATCAC 
AAATTTATGG CCTTTAAGTC ATTTGGAGAA TTGGTACATA AGCCATTACT GGTGGATCTC 
ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT 
GTTGATGTGG ATTCAGGATC AGTCTATGAC ATTTATCTAC CAACACATGT AAGAAAGAAC 
CCACACTCTA TGATCCAGTG TAGCATCAAA CCCCATGCAA TCATCATCCT CCCCAATACA 
GATGGAATGG AGCTTCTGGT GTGCTATGAA GATGAGGGGG TTTATGTAAA CACATATGGA 
AGGATCACCA AGGATGTAGT TCTACAGTGG GGAGAGATGC CTACATCAGT AGCATATATT 
CGATCCAATC AGACAATGGG CTGGGGAGAG AAGGCCATAG AGATCCGATC TGTGGAAACT 
GGTCACTTGG ATGGTGTGTT CATGCACAAA AGGGCTCAAA GACTAAAATT CTTGTGTGAA 
CGCAATGACA AGGTGTTCTT TGCCTCTGTT CGGTCTGGTG GCAGCAGTCA GGTTTATTTC 
ATGACCTTAG GCAGGACTTC TCTTCTGAGC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860840.1
CDS	75..4010
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005351.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005351.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG 
CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC 
TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC 
GGGGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG 
GAAAATGCAG TGAAAAAACC TGAAGATAAA AAGGAAGTTT TCAGACCCCT CAAGCCTGCT 
GATCTGACCG CACTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTACGGCC ACCTCACAAA 
GTAACGGACT ACTCCTCATC CAGTGAGGAG TCGGGGACGA CGGATGAGGA GGACGACGAT 
GTGGAGCAGG AAGGGGCTGA CGAGTCCACC TCAGGACCAG AGGACACCAG AGCAGCGTCA 
TCTCTGAATT TGAGCAATGG TGAAACGGAA TCTGTGAAAA CCATGATTGT CCATGATGAT 
GTAGAAAGTG AGCCGGCCAT GACCCCATCC AAGGAGGGCA CTCTAATCGT CCGCCAGAGT 
ACAGTTGACC AAAAGCGTGC CAGCCATCAT GAGAGCAATG GCTTTGCCGG TCGCATTCAC 
CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC 
CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTCACT 
GCTAATGAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGCGGAAC AACAGTGACA 
TCTGTGGTGG GATTTTCCTG TGATGGGATG AGACCAGAAG CCATAAGGCA AGATCCTACC 
CGGAAAGGCT CAGTGGTCAA TGTGAATCCT ACCAACACTA GGCCACAGAG TGACACCCCG 
GAGATTCGTA AATACAAGAA GAGGTTTAAC TCTGAGATTC TGTGTGCTGC CTTATGGGGA 
GTGAATTTGC TAGTGGGTAC AGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG 
AAGGTCTATC CTCTTATCAA CCGAAGACGA TTTCAACAAA TGGACGTACT TGAGGGCTTG 
AATGTCTTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG 
TTAAGAAATA AAATACTTCA CAATGATCCA GAAGTTGAGA AGAAGCAGGG ATGGACAACC 
GTAGGGGATT TGGAAGGATG TGTACATTAT AAAGTTGTAA AATATGAAAG AATCAAATTT 
CTGGTGATTG CTTTGAAGAG TTCTGTGGAA GTCTATGCGT GGGCACCAAA GCCATATCAC 
AAATTTATGG CCTTTAAGTC ATTTGGAGAA TTGGTACATA AGCCATTACT GGTGGATCTC 
ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT 
GTTGATGTGG ATTCAGGATC AGTCTATGAC ATTTATCTAC CAACACATGT AAGAAAGAAC 
CCACACTCTA TGATCCAGTG TAGCATCAAA CCCCATGCAA TCATCATCCT CCCCAATACA 
GATGGAATGG AGCTTCTGGT GTGCTATGAA GATGAGGGGG TTTATGTAAA CACATATGGA 
AGGATCACCA AGGATGTAGT TCTACAGTGG GGAGAGATGC CTACATCAGT AGCATATATT 
CGATCCAATC AGACAATGGG CTGGGGAGAG AAGGCCATAG AGATCCGATC TGTGGAAACT 
GGTCACTTGG ATGGTGTGTT CATGCACAAA AGGGCTCAAA GACTAAAATT CTTGTGTGAA 
CGCAATGACA AGGTGTTCTT TGCCTCTGTT CGGTCTGGTG GCAGCAGTCA GGTTTATTTC 
ATGACCTTAG GCAGGACTTC TCTTCTGAGC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005352.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860841.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu92775
Clone ID Related Accession (Same CDS sequence)	XM_017005352.1
Accession Version	XM_017005352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT 
GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC 
TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC 
TCCGGGGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC 
CTGGAAAATG CAGTGAAAAA ACCTGAAGAT AAAAAGGAAG TTTTCAGACC CCTCAAGCCT 
GCTGGCGAAG TGGATCTGAC CGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTACGG 
CCACCTCACA AAGTAACGGA CTACTCCTCA TCCAGTGAGG AGTCGGGGAC GACGGATGAG 
GAGGACGACG ATGTGGAGCA GGAAGGGGCT GACGAGTCCA CCTCAGGACC AGAGGACACC 
AGAGCAGCGT CATCTCTGAA TTTGAGCAAT GGTGAAACGG AATCTGTGAA AACCATGATT 
GTCCATGATG ATGTAGAAAG TGAGCCGGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC 
GTCCGCCAGA GTACAGTTGA CCAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCC 
GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC 
ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG 
GACAAGCTCA CTGCTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT 
TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGCGGA 
ACAACAGTGA CATCTGTGGT GGGATTTTCC TGTGATGGGA TGAGACCAGA AGCCATAAGG 
CAAGATCCTA CCCGGAAAGG CTCAGTGGTC AATGTGAATC CTACCAACAC TAGGCCACAG 
AGTGACACCC CGGAGATTCG TAAATACAAG AAGAGGTTTA ACTCTGAGAT TCTGTGTGCT 
GCCTTATGGG GAGTGAATTT GCTAGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA 
AGTGGCCAAG GGAAGGTCTA TCCTCTTATC AACCGAAGAC GATTTCAACA AATGGACGTA 
CTTGAGGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC 
TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAATGATC CAGAAGTTGA GAAGAAGCAG 
GGATGGACAA CCGTAGGGGA TTTGGAAGGA TGTGTACATT ATAAAGTTGT AAAATATGAA 
AGAATCAAAT TTCTGGTGAT TGCTTTGAAG AGTTCTGTGG AAGTCTATGC GTGGGCACCA 
AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AATTGGTACA TAAGCCATTA 
CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT 
GGATTCCATG CTGTTGATGT GGATTCAGGA TCAGTCTATG ACATTTATCT ACCAACACAT 
ATCCAGTGTA GCATCAAACC CCATGCAATC ATCATCCTCC CCAATACAGA TGGAATGGAG 
CTTCTGGTGT GCTATGAAGA TGAGGGGGTT TATGTAAACA CATATGGAAG GATCACCAAG 
GATGTAGTTC TACAGTGGGG AGAGATGCCT ACATCAGTAG CATATATTCG ATCCAATCAG 
ACAATGGGCT GGGGAGAGAA GGCCATAGAG ATCCGATCTG TGGAAACTGG TCACTTGGAT 
GGTGTGTTCA TGCACAAAAG GGCTCAAAGA CTAAAATTCT TGTGTGAACG CAATGACAAG 
GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC AGCAGTCAGG TTTATTTCAT GACCTTAGGC 
AGGACTTCTC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860841.1
CDS	75..3998
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005352.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005352.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT 
GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC 
TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC 
TCCGGGGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC 
CTGGAAAATG CAGTGAAAAA ACCTGAAGAT AAAAAGGAAG TTTTCAGACC CCTCAAGCCT 
GCTGGCGAAG TGGATCTGAC CGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTACGG 
CCACCTCACA AAGTAACGGA CTACTCCTCA TCCAGTGAGG AGTCGGGGAC GACGGATGAG 
GAGGACGACG ATGTGGAGCA GGAAGGGGCT GACGAGTCCA CCTCAGGACC AGAGGACACC 
AGAGCAGCGT CATCTCTGAA TTTGAGCAAT GGTGAAACGG AATCTGTGAA AACCATGATT 
GTCCATGATG ATGTAGAAAG TGAGCCGGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC 
GTCCGCCAGA GTACAGTTGA CCAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCC 
GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC 
ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG 
GACAAGCTCA CTGCTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT 
TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGCGGA 
ACAACAGTGA CATCTGTGGT GGGATTTTCC TGTGATGGGA TGAGACCAGA AGCCATAAGG 
CAAGATCCTA CCCGGAAAGG CTCAGTGGTC AATGTGAATC CTACCAACAC TAGGCCACAG 
AGTGACACCC CGGAGATTCG TAAATACAAG AAGAGGTTTA ACTCTGAGAT TCTGTGTGCT 
GCCTTATGGG GAGTGAATTT GCTAGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA 
AGTGGCCAAG GGAAGGTCTA TCCTCTTATC AACCGAAGAC GATTTCAACA AATGGACGTA 
CTTGAGGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC 
TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAATGATC CAGAAGTTGA GAAGAAGCAG 
GGATGGACAA CCGTAGGGGA TTTGGAAGGA TGTGTACATT ATAAAGTTGT AAAATATGAA 
AGAATCAAAT TTCTGGTGAT TGCTTTGAAG AGTTCTGTGG AAGTCTATGC GTGGGCACCA 
AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AATTGGTACA TAAGCCATTA 
CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT 
GGATTCCATG CTGTTGATGT GGATTCAGGA TCAGTCTATG ACATTTATCT ACCAACACAT 
ATCCAGTGTA GCATCAAACC CCATGCAATC ATCATCCTCC CCAATACAGA TGGAATGGAG 
CTTCTGGTGT GCTATGAAGA TGAGGGGGTT TATGTAAACA CATATGGAAG GATCACCAAG 
GATGTAGTTC TACAGTGGGG AGAGATGCCT ACATCAGTAG CATATATTCG ATCCAATCAG 
ACAATGGGCT GGGGAGAGAA GGCCATAGAG ATCCGATCTG TGGAAACTGG TCACTTGGAT 
GGTGTGTTCA TGCACAAAAG GGCTCAAAGA CTAAAATTCT TGTGTGAACG CAATGACAAG 
GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC AGCAGTCAGG TTTATTTCAT GACCTTAGGC 
AGGACTTCTC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005353.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860842.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu92776
Clone ID Related Accession (Same CDS sequence)	XM_017005353.1
Accession Version	XM_017005353.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG 
CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC 
TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC 
GGGGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG 
GAAAATGCAG TGAAAAAACC TGAAGATAAA AAGGAAGTTT TCAGACCCCT CAAGCCTGCT 
GGCGAAGTGG ATCTGACCGC ACTGGCCAAA GAGCTTCGAG CAGTGGAAGA TGTACGGCCA 
CCTCACAAAG TAACGGACTA CTCCTCATCC AGTGAGGAGT CGGGGACGAC GGATGAGGAG 
GACGACGATG TGGAGCAGGA AGGGGCTGAC GAGTCCACCT CAGGACCAGA GGACACCAGA 
GCAGCGTCAT CTCTGAATTT GAGCAATGGT GAAACGGAAT CTGTGAAAAC CATGATTGTC 
CATGATGATG TAGAAAGTGA GCCGGCCATG ACCCCATCCA AGGAGGGCAC TCTAATCGTC 
CGCCAGAGTA CAGTTGACCA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT 
CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC 
TCCTCCTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC 
AAGCTCACTG CTAATGAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC 
TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA 
ACAGTGACAT CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA 
GATCCTACCC GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT 
GACACCCCGG AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC 
TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT 
GGCCAAGGGA AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT 
GAGGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT 
TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA 
TGGACAACCG TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA 
ATCAAATTTC TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG 
CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG 
GTGGATCTCA CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA 
TTCCATGCTG TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATATC 
CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT 
CTGGTGTGCT ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT 
GTAGTTCTAC AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA 
ATGGGCTGGG GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT 
GTGTTCATGC ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG 
TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG 
ACTTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860842.1
CDS	75..3995
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005353.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005353.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG 
CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC 
TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC 
GGGGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG 
GAAAATGCAG TGAAAAAACC TGAAGATAAA AAGGAAGTTT TCAGACCCCT CAAGCCTGCT 
GGCGAAGTGG ATCTGACCGC ACTGGCCAAA GAGCTTCGAG CAGTGGAAGA TGTACGGCCA 
CCTCACAAAG TAACGGACTA CTCCTCATCC AGTGAGGAGT CGGGGACGAC GGATGAGGAG 
GACGACGATG TGGAGCAGGA AGGGGCTGAC GAGTCCACCT CAGGACCAGA GGACACCAGA 
GCAGCGTCAT CTCTGAATTT GAGCAATGGT GAAACGGAAT CTGTGAAAAC CATGATTGTC 
CATGATGATG TAGAAAGTGA GCCGGCCATG ACCCCATCCA AGGAGGGCAC TCTAATCGTC 
CGCCAGAGTA CAGTTGACCA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT 
CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC 
TCCTCCTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC 
AAGCTCACTG CTAATGAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC 
TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA 
ACAGTGACAT CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA 
GATCCTACCC GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT 
GACACCCCGG AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC 
TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT 
GGCCAAGGGA AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT 
GAGGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT 
TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA 
TGGACAACCG TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA 
ATCAAATTTC TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG 
CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG 
GTGGATCTCA CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA 
TTCCATGCTG TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATATC 
CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT 
CTGGTGTGCT ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT 
GTAGTTCTAC AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA 
ATGGGCTGGG GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT 
GTGTTCATGC ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG 
TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG 
ACTTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005354.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860843.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA.

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CloneID	OHu92777
Clone ID Related Accession (Same CDS sequence)	XM_017005354.1
Accession Version	XM_017005354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG 
TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC 
CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA 
AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA 
TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT 
AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA 
GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA 
TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA 
TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT 
GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA 
CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC 
TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA 
GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG 
AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG 
CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGAGTAC AGTTGACCAA 
AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCCGGTC GCATTCACCT CTTGCCAGAT 
CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCCTCCCC ATCCTCCAGC 
CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT 
CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA 
TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA 
GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA 
TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA 
GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT 
CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG 
ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG 
GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT 
TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC 
TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA 
GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT 
TCAGGATCAG TCTATGACAT TTATCTACCA ACACATATCC AGTGTAGCAT CAAACCCCAT 
GCAATCATCA TCCTCCCCAA TACAGATGGA ATGGAGCTTC TGGTGTGCTA TGAAGATGAG 
GGGGTTTATG TAAACACATA TGGAAGGATC ACCAAGGATG TAGTTCTACA GTGGGGAGAG 
ATGCCTACAT CAGTAGCATA TATTCGATCC AATCAGACAA TGGGCTGGGG AGAGAAGGCC 
ATAGAGATCC GATCTGTGGA AACTGGTCAC TTGGATGGTG TGTTCATGCA CAAAAGGGCT 
CAAAGACTAA AATTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC TGTTCGGTCT 
GGTGGCAGCA GTCAGGTTTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860843.1
CDS	75..3974
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005354.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005354.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG 
TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC 
CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA 
AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA 
TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT 
AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA 
GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA 
TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA 
TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT 
GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA 
CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC 
TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA 
GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG 
AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG 
CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGAGTAC AGTTGACCAA 
AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCCGGTC GCATTCACCT CTTGCCAGAT 
CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCCTCCCC ATCCTCCAGC 
CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT 
CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA 
TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA 
GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA 
TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA 
GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT 
CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG 
ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG 
GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT 
TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC 
TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA 
GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT 
TCAGGATCAG TCTATGACAT TTATCTACCA ACACATATCC AGTGTAGCAT CAAACCCCAT 
GCAATCATCA TCCTCCCCAA TACAGATGGA ATGGAGCTTC TGGTGTGCTA TGAAGATGAG 
GGGGTTTATG TAAACACATA TGGAAGGATC ACCAAGGATG TAGTTCTACA GTGGGGAGAG 
ATGCCTACAT CAGTAGCATA TATTCGATCC AATCAGACAA TGGGCTGGGG AGAGAAGGCC 
ATAGAGATCC GATCTGTGGA AACTGGTCAC TTGGATGGTG TGTTCATGCA CAAAAGGGCT 
CAAAGACTAA AATTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC TGTTCGGTCT 
GGTGGCAGCA GTCAGGTTTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005355.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860844.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu92778
Clone ID Related Accession (Same CDS sequence)	XM_017005355.1
Accession Version	XM_017005355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG 
TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC 
CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA 
AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA 
TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT 
AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA 
GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA 
TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA 
TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT 
GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG ATCTGACCGC ACTGGCCAAA 
GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC 
AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC 
GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT 
GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG 
ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGAGTA CAGTTGACCA AAAGCGTGCC 
AGCCATCATG AGAGCAATGG CTTTGCCGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG 
CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCCTCCC CATCCTCCAG CCAGCCGACA 
CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTG CTAATGAGAC TCAGTCCGCT 
AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
TTACTACAGA TTTCTCCATC TAGCGGAACA ACAGTGACAT CTGTGGTGGG ATTTTCCTGT 
GATGGGATGA GACCAGAAGC CATAAGGCAA GATCCTACCC GGAAAGGCTC AGTGGTCAAT 
GTGAATCCTA CCAACACTAG GCCACAGAGT GACACCCCGG AGATTCGTAA ATACAAGAAG 
AGGTTTAACT CTGAGATTCT GTGTGCTGCC TTATGGGGAG TGAATTTGCT AGTGGGTACA 
GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTCTATCC TCTTATCAAC 
CGAAGACGAT TTCAACAAAT GGACGTACTT GAGGGCTTGA ATGTCTTGGT GACAATATCT 
GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACAACCG TAGGGGATTT GGAAGGATGT 
GTACATTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC TGGTGATTGC TTTGAAGAGT 
TCTGTGGAAG TCTATGCGTG GGCACCAAAG CCATATCACA AATTTATGGC CTTTAAGTCA 
TTTGGAGAAT TGGTACATAA GCCATTACTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA TTCAGGATCA 
GTCTATGACA TTTATCTACC AACACATATC CAGTGTAGCA TCAAACCCCA TGCAATCATC 
ATCCTCCCCA ATACAGATGG AATGGAGCTT CTGGTGTGCT ATGAAGATGA GGGGGTTTAT 
GTAAACACAT ATGGAAGGAT CACCAAGGAT GTAGTTCTAC AGTGGGGAGA GATGCCTACA 
TCAGTAGCAT ATATTCGATC CAATCAGACA ATGGGCTGGG GAGAGAAGGC CATAGAGATC 
CGATCTGTGG AAACTGGTCA CTTGGATGGT GTGTTCATGC ACAAAAGGGC TCAAAGACTA 
AAATTCTTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC 
AGTCAGGTTT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860844.1
CDS	75..3965
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005355.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005355.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG 
TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC 
CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA 
AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA 
TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT 
AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA 
GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA 
TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA 
TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT 
GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG ATCTGACCGC ACTGGCCAAA 
GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC 
AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC 
GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT 
GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG 
ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGAGTA CAGTTGACCA AAAGCGTGCC 
AGCCATCATG AGAGCAATGG CTTTGCCGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG 
CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCCTCCC CATCCTCCAG CCAGCCGACA 
CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTG CTAATGAGAC TCAGTCCGCT 
AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
TTACTACAGA TTTCTCCATC TAGCGGAACA ACAGTGACAT CTGTGGTGGG ATTTTCCTGT 
GATGGGATGA GACCAGAAGC CATAAGGCAA GATCCTACCC GGAAAGGCTC AGTGGTCAAT 
GTGAATCCTA CCAACACTAG GCCACAGAGT GACACCCCGG AGATTCGTAA ATACAAGAAG 
AGGTTTAACT CTGAGATTCT GTGTGCTGCC TTATGGGGAG TGAATTTGCT AGTGGGTACA 
GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTCTATCC TCTTATCAAC 
CGAAGACGAT TTCAACAAAT GGACGTACTT GAGGGCTTGA ATGTCTTGGT GACAATATCT 
GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACAACCG TAGGGGATTT GGAAGGATGT 
GTACATTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC TGGTGATTGC TTTGAAGAGT 
TCTGTGGAAG TCTATGCGTG GGCACCAAAG CCATATCACA AATTTATGGC CTTTAAGTCA 
TTTGGAGAAT TGGTACATAA GCCATTACTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA TTCAGGATCA 
GTCTATGACA TTTATCTACC AACACATATC CAGTGTAGCA TCAAACCCCA TGCAATCATC 
ATCCTCCCCA ATACAGATGG AATGGAGCTT CTGGTGTGCT ATGAAGATGA GGGGGTTTAT 
GTAAACACAT ATGGAAGGAT CACCAAGGAT GTAGTTCTAC AGTGGGGAGA GATGCCTACA 
TCAGTAGCAT ATATTCGATC CAATCAGACA ATGGGCTGGG GAGAGAAGGC CATAGAGATC 
CGATCTGTGG AAACTGGTCA CTTGGATGGT GTGTTCATGC ACAAAAGGGC TCAAAGACTA 
AAATTCTTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC 
AGTCAGGTTT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005356.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860845.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHu92779
Clone ID Related Accession (Same CDS sequence)	XM_017005356.1
Accession Version	XM_017005356.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA 
AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC 
TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT 
GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA 
CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
ATTTCTCCAT CTAGCGGAAC AACAGTGACA TCTGTGGTGG GATTTTCCTG TGATGGGATG 
AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT 
ACCAACACTA GGCCACAGAG TGACACCCCG GAGATTCGTA AATACAAGAA GAGGTTTAAC 
TCTGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC 
CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTTATCAA CCGAAGACGA 
TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG 
GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA 
GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT 
AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAGAG TTCTGTGGAA 
GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA 
TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG 
ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCAGGATC AGTCTATGAC 
ATTTATCTAC CAACACATGT AAGAAAGAAC CCACACTCTA TGATCCAGTG TAGCATCAAA 
CCCCATGCAA TCATCATCCT CCCCAATACA GATGGAATGG AGCTTCTGGT GTGCTATGAA 
GATGAGGGGG TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTAGT TCTACAGTGG 
GGAGAGATGC CTACATCAGT AGCATATATT CGATCCAATC AGACAATGGG CTGGGGAGAG 
AAGGCCATAG AGATCCGATC TGTGGAAACT GGTCACTTGG ATGGTGTGTT CATGCACAAA 
AGGGCTCAAA GACTAAAATT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCTGTT 
CGGTCTGGTG GCAGCAGTCA GGTTTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860845.1
CDS	75..3920
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005356.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005356.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA 
AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC 
TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT 
GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA 
CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
ATTTCTCCAT CTAGCGGAAC AACAGTGACA TCTGTGGTGG GATTTTCCTG TGATGGGATG 
AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT 
ACCAACACTA GGCCACAGAG TGACACCCCG GAGATTCGTA AATACAAGAA GAGGTTTAAC 
TCTGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC 
CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTTATCAA CCGAAGACGA 
TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG 
GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA 
GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT 
AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAGAG TTCTGTGGAA 
GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA 
TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG 
ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCAGGATC AGTCTATGAC 
ATTTATCTAC CAACACATGT AAGAAAGAAC CCACACTCTA TGATCCAGTG TAGCATCAAA 
CCCCATGCAA TCATCATCCT CCCCAATACA GATGGAATGG AGCTTCTGGT GTGCTATGAA 
GATGAGGGGG TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTAGT TCTACAGTGG 
GGAGAGATGC CTACATCAGT AGCATATATT CGATCCAATC AGACAATGGG CTGGGGAGAG 
AAGGCCATAG AGATCCGATC TGTGGAAACT GGTCACTTGG ATGGTGTGTT CATGCACAAA 
AGGGCTCAAA GACTAAAATT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCTGTT 
CGGTCTGGTG GCAGCAGTCA GGTTTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005357.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860846.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA.

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CloneID	OHu92780
Clone ID Related Accession (Same CDS sequence)	XM_017005357.1
Accession Version	XM_017005357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA 
AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA 
GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC 
AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC 
GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT 
GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG 
ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGAGTA CAGTTGACCA AAAGCGTGCC 
AGCCATCATG AGAGCAATGG CTTTGCCGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG 
CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCCTCCC CATCCTCCAG CCAGCCGACA 
CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTG CTAATGAGAC TCAGTCCGCT 
AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
TTACTACAGA TTTCTCCATC TAGCGGAACA ACAGTGACAT CTGTGGTGGG ATTTTCCTGT 
GATGGGATGA GACCAGAAGC CATAAGGCAA GATCCTACCC GGAAAGGCTC AGTGGTCAAT 
GTGAATCCTA CCAACACTAG GCCACAGAGT GACACCCCGG AGATTCGTAA ATACAAGAAG 
AGGTTTAACT CTGAGATTCT GTGTGCTGCC TTATGGGGAG TGAATTTGCT AGTGGGTACA 
GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTCTATCC TCTTATCAAC 
CGAAGACGAT TTCAACAAAT GGACGTACTT GAGGGCTTGA ATGTCTTGGT GACAATATCT 
GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACAACCG TAGGGGATTT GGAAGGATGT 
GTACATTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC TGGTGATTGC TTTGAAGAGT 
TCTGTGGAAG TCTATGCGTG GGCACCAAAG CCATATCACA AATTTATGGC CTTTAAGTCA 
TTTGGAGAAT TGGTACATAA GCCATTACTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA TTCAGGATCA 
GTCTATGACA TTTATCTACC AACACATATC CAGTGTAGCA TCAAACCCCA TGCAATCATC 
ATCCTCCCCA ATACAGATGG AATGGAGCTT CTGGTGTGCT ATGAAGATGA GGGGGTTTAT 
GTAAACACAT ATGGAAGGAT CACCAAGGAT GTAGTTCTAC AGTGGGGAGA GATGCCTACA 
TCAGTAGCAT ATATTCGATC CAATCAGACA ATGGGCTGGG GAGAGAAGGC CATAGAGATC 
CGATCTGTGG AAACTGGTCA CTTGGATGGT GTGTTCATGC ACAAAAGGGC TCAAAGACTA 
AAATTCTTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC 
AGTCAGGTTT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860846.1
CDS	75..3905
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005357.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005357.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA 
AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA 
GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC 
AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC 
GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT 
GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG 
ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGAGTA CAGTTGACCA AAAGCGTGCC 
AGCCATCATG AGAGCAATGG CTTTGCCGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG 
CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCCTCCC CATCCTCCAG CCAGCCGACA 
CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTG CTAATGAGAC TCAGTCCGCT 
AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
TTACTACAGA TTTCTCCATC TAGCGGAACA ACAGTGACAT CTGTGGTGGG ATTTTCCTGT 
GATGGGATGA GACCAGAAGC CATAAGGCAA GATCCTACCC GGAAAGGCTC AGTGGTCAAT 
GTGAATCCTA CCAACACTAG GCCACAGAGT GACACCCCGG AGATTCGTAA ATACAAGAAG 
AGGTTTAACT CTGAGATTCT GTGTGCTGCC TTATGGGGAG TGAATTTGCT AGTGGGTACA 
GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTCTATCC TCTTATCAAC 
CGAAGACGAT TTCAACAAAT GGACGTACTT GAGGGCTTGA ATGTCTTGGT GACAATATCT 
GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACAACCG TAGGGGATTT GGAAGGATGT 
GTACATTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC TGGTGATTGC TTTGAAGAGT 
TCTGTGGAAG TCTATGCGTG GGCACCAAAG CCATATCACA AATTTATGGC CTTTAAGTCA 
TTTGGAGAAT TGGTACATAA GCCATTACTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA TTCAGGATCA 
GTCTATGACA TTTATCTACC AACACATATC CAGTGTAGCA TCAAACCCCA TGCAATCATC 
ATCCTCCCCA ATACAGATGG AATGGAGCTT CTGGTGTGCT ATGAAGATGA GGGGGTTTAT 
GTAAACACAT ATGGAAGGAT CACCAAGGAT GTAGTTCTAC AGTGGGGAGA GATGCCTACA 
TCAGTAGCAT ATATTCGATC CAATCAGACA ATGGGCTGGG GAGAGAAGGC CATAGAGATC 
CGATCTGTGG AAACTGGTCA CTTGGATGGT GTGTTCATGC ACAAAAGGGC TCAAAGACTA 
AAATTCTTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC 
AGTCAGGTTT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005358.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860847.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OHu92781
Clone ID Related Accession (Same CDS sequence)	XM_017005358.1
Accession Version	XM_017005358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG TATTGAGCCC AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG 
GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA GGGTCCAGCA ACTCAGGATC CCAGCCCGGG 
TCTCACCCTG GGTCTCAGAG TGGCTCCGGG GAACGCTTCA GAGTGAGATC ATCATCCAAG 
TCTGAAGGCT CTCCATCTCA GCGCCTGGAA AATGCAGTGA AAAAACCTGA AGATAAAAAG 
GAAGTTTTCA GACCCCTCAA GCCTGCTGGC GAAGTGGATC TGACCGCACT GGCCAAAGAG 
CTTCGAGCAG TGGAAGATGT ACGGCCACCT CACAAAGTAA CGGACTACTC CTCATCCAGT 
GAGGAGTCGG GGACGACGGA TGAGGAGGAC GACGATGTGG AGCAGGAAGG GGCTGACGAG 
TCCACCTCAG GACCAGAGGA CACCAGAGCA GCGTCATCTC TGAATTTGAG CAATGGTGAA 
ACGGAATCTG TGAAAACCAT GATTGTCCAT GATGATGTAG AAAGTGAGCC GGCCATGACC 
CCATCCAAGG AGGGCACTCT AATCGTCCGC CAGAGTACAG TTGACCAAAA GCGTGCCAGC 
CATCATGAGA GCAATGGCTT TGCCGGTCGC ATTCACCTCT TGCCAGATCT CTTACAGCAA 
AGCCATTCCT CCTCCACTTC CTCCACCTCC TCCTCCCCAT CCTCCAGCCA GCCGACACCC 
ACCATGTCCC CACAGACACC CCAGGACAAG CTCACTGCTA ATGAGACTCA GTCCGCTAGT 
AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA 
CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT 
GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG 
AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG 
TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG 
AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA 
AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC 
AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT 
GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA 
CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT 
GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT 
GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG 
AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC 
TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA AACCCCATGC AATCATCATC 
CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA 
AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA 
GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA 
TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA 
TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT 
CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860847.1
CDS	75..3902
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005358.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005358.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG TATTGAGCCC AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG 
GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA GGGTCCAGCA ACTCAGGATC CCAGCCCGGG 
TCTCACCCTG GGTCTCAGAG TGGCTCCGGG GAACGCTTCA GAGTGAGATC ATCATCCAAG 
TCTGAAGGCT CTCCATCTCA GCGCCTGGAA AATGCAGTGA AAAAACCTGA AGATAAAAAG 
GAAGTTTTCA GACCCCTCAA GCCTGCTGGC GAAGTGGATC TGACCGCACT GGCCAAAGAG 
CTTCGAGCAG TGGAAGATGT ACGGCCACCT CACAAAGTAA CGGACTACTC CTCATCCAGT 
GAGGAGTCGG GGACGACGGA TGAGGAGGAC GACGATGTGG AGCAGGAAGG GGCTGACGAG 
TCCACCTCAG GACCAGAGGA CACCAGAGCA GCGTCATCTC TGAATTTGAG CAATGGTGAA 
ACGGAATCTG TGAAAACCAT GATTGTCCAT GATGATGTAG AAAGTGAGCC GGCCATGACC 
CCATCCAAGG AGGGCACTCT AATCGTCCGC CAGAGTACAG TTGACCAAAA GCGTGCCAGC 
CATCATGAGA GCAATGGCTT TGCCGGTCGC ATTCACCTCT TGCCAGATCT CTTACAGCAA 
AGCCATTCCT CCTCCACTTC CTCCACCTCC TCCTCCCCAT CCTCCAGCCA GCCGACACCC 
ACCATGTCCC CACAGACACC CCAGGACAAG CTCACTGCTA ATGAGACTCA GTCCGCTAGT 
AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA 
CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT 
GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG 
AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG 
TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG 
AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA 
AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC 
AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT 
GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA 
CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT 
GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT 
GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG 
AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC 
TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA AACCCCATGC AATCATCATC 
CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA 
AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA 
GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA 
TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA 
TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT 
CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005359.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860848.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OHu92782
Clone ID Related Accession (Same CDS sequence)	XM_017005359.1
Accession Version	XM_017005359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC 
CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT 
GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC 
GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA 
GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC 
AGCAGTATTG AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC 
AGTGGCAGCT CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT 
CAGAGTGGCT CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA 
TCTCAGCGCC TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC 
CTCAAGCCTG CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA 
GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG 
ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA 
GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA 
ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC 
ACTCTAATCG TCCGCCAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC 
TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA 
ACAGTGACAT CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA 
GATCCTACCC GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT 
GACACCCCGG AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC 
TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT 
GGCCAAGGGA AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT 
GAGGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT 
TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA 
TGGACAACCG TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA 
ATCAAATTTC TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG 
CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG 
GTGGATCTCA CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA 
TTCCATGCTG TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATGTA 
AGAAAGAACC CACACTCTAT GATCCAGTGT AGCATCAAAC CCCATGCAAT CATCATCCTC 
CCCAATACAG ATGGAATGGA GCTTCTGGTG TGCTATGAAG ATGAGGGGGT TTATGTAAAC 
ACATATGGAA GGATCACCAA GGATGTAGTT CTACAGTGGG GAGAGATGCC TACATCAGTA 
GCATATATTC GATCCAATCA GACAATGGGC TGGGGAGAGA AGGCCATAGA GATCCGATCT 
GTGGAAACTG GTCACTTGGA TGGTGTGTTC ATGCACAAAA GGGCTCAAAG ACTAAAATTC 
TTGTGTGAAC GCAATGACAA GGTGTTCTTT GCCTCTGTTC GGTCTGGTGG CAGCAGTCAG 
GTTTATTTCA TGACCTTAGG CAGGACTTCT CTTCTGAGCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860848.1
CDS	75..3899
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005359.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005359.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC 
CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT 
GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC 
GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA 
GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC 
AGCAGTATTG AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC 
AGTGGCAGCT CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT 
CAGAGTGGCT CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA 
TCTCAGCGCC TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC 
CTCAAGCCTG CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA 
GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG 
ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA 
GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA 
ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC 
ACTCTAATCG TCCGCCAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC 
TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA 
ACAGTGACAT CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA 
GATCCTACCC GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT 
GACACCCCGG AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC 
TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT 
GGCCAAGGGA AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT 
GAGGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT 
TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA 
TGGACAACCG TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA 
ATCAAATTTC TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG 
CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG 
GTGGATCTCA CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA 
TTCCATGCTG TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATGTA 
AGAAAGAACC CACACTCTAT GATCCAGTGT AGCATCAAAC CCCATGCAAT CATCATCCTC 
CCCAATACAG ATGGAATGGA GCTTCTGGTG TGCTATGAAG ATGAGGGGGT TTATGTAAAC 
ACATATGGAA GGATCACCAA GGATGTAGTT CTACAGTGGG GAGAGATGCC TACATCAGTA 
GCATATATTC GATCCAATCA GACAATGGGC TGGGGAGAGA AGGCCATAGA GATCCGATCT 
GTGGAAACTG GTCACTTGGA TGGTGTGTTC ATGCACAAAA GGGCTCAAAG ACTAAAATTC 
TTGTGTGAAC GCAATGACAA GGTGTTCTTT GCCTCTGTTC GGTCTGGTGG CAGCAGTCAG 
GTTTATTTCA TGACCTTAGG CAGGACTTCT CTTCTGAGCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005360.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860849.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X20, mRNA.

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CloneID	OHu92783
Clone ID Related Accession (Same CDS sequence)	XM_017005360.1
Accession Version	XM_017005360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC 
CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT 
GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC 
GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA 
GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC 
AGTATTGAGC CCAGGCTTCT GTGGGAGAGA GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT 
GGCAGCTCCT CAGGGTCCAG CAACTCAGGA TCCCAGCCCG GGTCTCACCC TGGGTCTCAG 
AGTGGCTCCG GGGAACGCTT CAGAGTGAGA TCATCATCCA AGTCTGAAGG CTCTCCATCT 
CAGCGCCTGG AAAATGCAGT GAAAAAACCT GAAGATAAAA AGGAAGTTTT CAGACCCCTC 
AAGCCTGCTG GCGAAGTGGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT 
GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG 
GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG 
GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC 
ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT 
CTAATCGTCC GCCAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC 
TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGAACAACA 
GTGACATCTG TGGTGGGATT TTCCTGTGAT GGGATGAGAC CAGAAGCCAT AAGGCAAGAT 
CCTACCCGGA AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAC 
ACCCCGGAGA TTCGTAAATA CAAGAAGAGG TTTAACTCTG AGATTCTGTG TGCTGCCTTA 
TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC 
CAAGGGAAGG TCTATCCTCT TATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG 
GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG 
TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG 
ACAACCGTAG GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC 
AAATTTCTGG TGATTGCTTT GAAGAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA 
TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG 
GATCTCACTG TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC 
CATGCTGTTG ATGTGGATTC AGGATCAGTC TATGACATTT ATCTACCAAC ACATGTAAGA 
AAGAACCCAC ACTCTATGAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC 
AATACAGATG GAATGGAGCT TCTGGTGTGC TATGAAGATG AGGGGGTTTA TGTAAACACA 
TATGGAAGGA TCACCAAGGA TGTAGTTCTA CAGTGGGGAG AGATGCCTAC ATCAGTAGCA 
TATATTCGAT CCAATCAGAC AATGGGCTGG GGAGAGAAGG CCATAGAGAT CCGATCTGTG 
GAAACTGGTC ACTTGGATGG TGTGTTCATG CACAAAAGGG CTCAAAGACT AAAATTCTTG 
TGTGAACGCA ATGACAAGGT GTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT 
TATTTCATGA CCTTAGGCAG GACTTCTCTT CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860849.1
CDS	75..3896
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005360.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005360.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC 
CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT 
GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC 
GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA 
GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC 
AGTATTGAGC CCAGGCTTCT GTGGGAGAGA GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT 
GGCAGCTCCT CAGGGTCCAG CAACTCAGGA TCCCAGCCCG GGTCTCACCC TGGGTCTCAG 
AGTGGCTCCG GGGAACGCTT CAGAGTGAGA TCATCATCCA AGTCTGAAGG CTCTCCATCT 
CAGCGCCTGG AAAATGCAGT GAAAAAACCT GAAGATAAAA AGGAAGTTTT CAGACCCCTC 
AAGCCTGCTG GCGAAGTGGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT 
GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG 
GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG 
GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC 
ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT 
CTAATCGTCC GCCAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC 
TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGAACAACA 
GTGACATCTG TGGTGGGATT TTCCTGTGAT GGGATGAGAC CAGAAGCCAT AAGGCAAGAT 
CCTACCCGGA AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAC 
ACCCCGGAGA TTCGTAAATA CAAGAAGAGG TTTAACTCTG AGATTCTGTG TGCTGCCTTA 
TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC 
CAAGGGAAGG TCTATCCTCT TATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG 
GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG 
TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG 
ACAACCGTAG GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC 
AAATTTCTGG TGATTGCTTT GAAGAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA 
TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG 
GATCTCACTG TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC 
CATGCTGTTG ATGTGGATTC AGGATCAGTC TATGACATTT ATCTACCAAC ACATGTAAGA 
AAGAACCCAC ACTCTATGAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC 
AATACAGATG GAATGGAGCT TCTGGTGTGC TATGAAGATG AGGGGGTTTA TGTAAACACA 
TATGGAAGGA TCACCAAGGA TGTAGTTCTA CAGTGGGGAG AGATGCCTAC ATCAGTAGCA 
TATATTCGAT CCAATCAGAC AATGGGCTGG GGAGAGAAGG CCATAGAGAT CCGATCTGTG 
GAAACTGGTC ACTTGGATGG TGTGTTCATG CACAAAAGGG CTCAAAGACT AAAATTCTTG 
TGTGAACGCA ATGACAAGGT GTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT 
TATTTCATGA CCTTAGGCAG GACTTCTCTT CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005361.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860850.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OHu92784
Clone ID Related Accession (Same CDS sequence)	XM_017005361.1
Accession Version	XM_017005361.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC 
CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT 
GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC 
GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA 
GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC 
AGCAGTATTG AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC 
AGTGGCAGCT CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT 
CAGAGTGGCT CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA 
TCTCAGCGCC TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC 
CTCAAGCCTG CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA 
GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG 
ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA 
GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA 
ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC 
ACTCTAATCG TCCGCCAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC 
TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA 
ACAGTGACAT CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA 
GATCCTACCC GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT 
GACACCCCGG AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC 
TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT 
GGCCAAGGGA AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT 
GAGGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT 
TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA 
TGGACAACCG TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA 
ATCAAATTTC TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG 
CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG 
GTGGATCTCA CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA 
TTCCATGCTG TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATATC 
CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT 
CTGGTGTGCT ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT 
GTAGTTCTAC AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA 
ATGGGCTGGG GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT 
GTGTTCATGC ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG 
TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG 
ACTTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860850.1
CDS	75..3875
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005361.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005361.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC 
CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT 
GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC 
GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA 
GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC 
AGCAGTATTG AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC 
AGTGGCAGCT CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT 
CAGAGTGGCT CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA 
TCTCAGCGCC TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC 
CTCAAGCCTG CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA 
GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG 
ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA 
GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA 
ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC 
ACTCTAATCG TCCGCCAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC 
TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA 
ACAGTGACAT CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA 
GATCCTACCC GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT 
GACACCCCGG AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC 
TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT 
GGCCAAGGGA AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT 
GAGGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT 
TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA 
TGGACAACCG TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA 
ATCAAATTTC TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG 
CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG 
GTGGATCTCA CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA 
TTCCATGCTG TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATATC 
CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT 
CTGGTGTGCT ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT 
GTAGTTCTAC AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA 
ATGGGCTGGG GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT 
GTGTTCATGC ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG 
TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG 
ACTTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005362.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860851.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X22, mRNA.

Price & Availability↑

CloneID	OHu92785
Clone ID Related Accession (Same CDS sequence)	XM_017005362.1
Accession Version	XM_017005362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC 
CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT 
GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC 
GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA 
GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC 
AGTATTGAGC CCAGGCTTCT GTGGGAGAGA GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT 
GGCAGCTCCT CAGGGTCCAG CAACTCAGGA TCCCAGCCCG GGTCTCACCC TGGGTCTCAG 
AGTGGCTCCG GGGAACGCTT CAGAGTGAGA TCATCATCCA AGTCTGAAGG CTCTCCATCT 
CAGCGCCTGG AAAATGCAGT GAAAAAACCT GAAGATAAAA AGGAAGTTTT CAGACCCCTC 
AAGCCTGCTG GCGAAGTGGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT 
GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG 
GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG 
GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC 
ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT 
CTAATCGTCC GCCAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC 
TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGAACAACA 
GTGACATCTG TGGTGGGATT TTCCTGTGAT GGGATGAGAC CAGAAGCCAT AAGGCAAGAT 
CCTACCCGGA AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAC 
ACCCCGGAGA TTCGTAAATA CAAGAAGAGG TTTAACTCTG AGATTCTGTG TGCTGCCTTA 
TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC 
CAAGGGAAGG TCTATCCTCT TATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG 
GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG 
TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG 
ACAACCGTAG GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC 
AAATTTCTGG TGATTGCTTT GAAGAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA 
TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG 
GATCTCACTG TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC 
CATGCTGTTG ATGTGGATTC AGGATCAGTC TATGACATTT ATCTACCAAC ACATATCCAG 
TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG 
GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA 
GTTCTACAGT GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG 
GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG 
TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC 
TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT 
TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860851.1
CDS	75..3872
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005362.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005362.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC 
CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT 
GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC 
GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA 
GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC 
AGTATTGAGC CCAGGCTTCT GTGGGAGAGA GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT 
GGCAGCTCCT CAGGGTCCAG CAACTCAGGA TCCCAGCCCG GGTCTCACCC TGGGTCTCAG 
AGTGGCTCCG GGGAACGCTT CAGAGTGAGA TCATCATCCA AGTCTGAAGG CTCTCCATCT 
CAGCGCCTGG AAAATGCAGT GAAAAAACCT GAAGATAAAA AGGAAGTTTT CAGACCCCTC 
AAGCCTGCTG GCGAAGTGGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT 
GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG 
GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG 
GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC 
ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT 
CTAATCGTCC GCCAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC 
TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGAACAACA 
GTGACATCTG TGGTGGGATT TTCCTGTGAT GGGATGAGAC CAGAAGCCAT AAGGCAAGAT 
CCTACCCGGA AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAC 
ACCCCGGAGA TTCGTAAATA CAAGAAGAGG TTTAACTCTG AGATTCTGTG TGCTGCCTTA 
TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC 
CAAGGGAAGG TCTATCCTCT TATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG 
GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG 
TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG 
ACAACCGTAG GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC 
AAATTTCTGG TGATTGCTTT GAAGAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA 
TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG 
GATCTCACTG TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC 
CATGCTGTTG ATGTGGATTC AGGATCAGTC TATGACATTT ATCTACCAAC ACATATCCAG 
TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG 
GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA 
GTTCTACAGT GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG 
GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG 
TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC 
TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT 
TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005363.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860852.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X26, mRNA.

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CloneID	OHu92786
Clone ID Related Accession (Same CDS sequence)	XM_017005363.1
Accession Version	XM_017005363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC 
AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC 
CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA 
GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT 
TTCAGACCCC TCAAGCCTGC TGGCGAAGTG GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC 
TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT 
GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA 
CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
ATTTCTCCAT CTAGCGGAAC AACAGTGACA TCTGTGGTGG GATTTTCCTG TGATGGGATG 
AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT 
ACCAACACTA GGCCACAGAG TGACACCCCG GAGATTCGTA AATACAAGAA GAGGTTTAAC 
TCTGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC 
CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTTATCAA CCGAAGACGA 
TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG 
GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA 
GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT 
AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAGAG TTCTGTGGAA 
GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA 
TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG 
ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCAGGATC AGTCTATGAC 
ATTTATCTAC CAACACATAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC 
AATACAGATG GAATGGAGCT TCTGGTGTGC TATGAAGATG AGGGGGTTTA TGTAAACACA 
TATGGAAGGA TCACCAAGGA TGTAGTTCTA CAGTGGGGAG AGATGCCTAC ATCAGTAGCA 
TATATTCGAT CCAATCAGAC AATGGGCTGG GGAGAGAAGG CCATAGAGAT CCGATCTGTG 
GAAACTGGTC ACTTGGATGG TGTGTTCATG CACAAAAGGG CTCAAAGACT AAAATTCTTG 
TGTGAACGCA ATGACAAGGT GTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT 
TATTTCATGA CCTTAGGCAG GACTTCTCTT CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860852.1
CDS	75..3836
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005363.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005363.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC 
AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC 
CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA 
GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT 
TTCAGACCCC TCAAGCCTGC TGGCGAAGTG GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC 
TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT 
GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA 
CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
ATTTCTCCAT CTAGCGGAAC AACAGTGACA TCTGTGGTGG GATTTTCCTG TGATGGGATG 
AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT 
ACCAACACTA GGCCACAGAG TGACACCCCG GAGATTCGTA AATACAAGAA GAGGTTTAAC 
TCTGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC 
CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTTATCAA CCGAAGACGA 
TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG 
GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA 
GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT 
AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAGAG TTCTGTGGAA 
GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA 
TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG 
ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCAGGATC AGTCTATGAC 
ATTTATCTAC CAACACATAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC 
AATACAGATG GAATGGAGCT TCTGGTGTGC TATGAAGATG AGGGGGTTTA TGTAAACACA 
TATGGAAGGA TCACCAAGGA TGTAGTTCTA CAGTGGGGAG AGATGCCTAC ATCAGTAGCA 
TATATTCGAT CCAATCAGAC AATGGGCTGG GGAGAGAAGG CCATAGAGAT CCGATCTGTG 
GAAACTGGTC ACTTGGATGG TGTGTTCATG CACAAAAGGG CTCAAAGACT AAAATTCTTG 
TGTGAACGCA ATGACAAGGT GTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT 
TATTTCATGA CCTTAGGCAG GACTTCTCTT CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005364.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860853.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OHu92787
Clone ID Related Accession (Same CDS sequence)	XM_017005364.1
Accession Version	XM_017005364.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA 
CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT 
GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC 
TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC 
AGACCCCTCA AGCCTGCTGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT 
GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG 
GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG 
GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC 
ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT 
CTAATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC 
TTTGCCGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT 
TCCTCCACCT CCTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA 
CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC 
AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
AGCGGAACAA CAGTGACATC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCAGAAGCC 
ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG 
CCACAGAGTG ACACCCCGGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAGATTCTG 
TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG 
GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTTATCAACC GAAGACGATT TCAACAAATG 
GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT 
GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG 
AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA 
TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCGTGG 
GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG 
CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC 
TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCAGGATCAG TCTATGACAT TTATCTACCA 
ACACATATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA 
ATGGAGCTTC TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC 
ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACAT CAGTAGCATA TATTCGATCC 
AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC 
TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT 
GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC 
TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860853.1
CDS	75..3824
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005364.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005364.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA 
CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT 
GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC 
TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC 
AGACCCCTCA AGCCTGCTGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT 
GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG 
GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG 
GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC 
ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT 
CTAATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC 
TTTGCCGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT 
TCCTCCACCT CCTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA 
CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC 
AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
AGCGGAACAA CAGTGACATC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCAGAAGCC 
ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG 
CCACAGAGTG ACACCCCGGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAGATTCTG 
TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG 
GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTTATCAACC GAAGACGATT TCAACAAATG 
GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT 
GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG 
AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA 
TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCGTGG 
GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG 
CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC 
TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCAGGATCAG TCTATGACAT TTATCTACCA 
ACACATATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA 
ATGGAGCTTC TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC 
ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACAT CAGTAGCATA TATTCGATCC 
AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC 
TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT 
GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC 
TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005365.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860854.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OHu92788
Clone ID Related Accession (Same CDS sequence)	XM_017005365.1
Accession Version	XM_017005365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT 
GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC 
TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC 
TCCGGGGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC 
CTGGAAAATG CAGTGAAAAA ACCTGAAGAT AAAAAGGAAG TTTTCAGACC CCTCAAGCCT 
GCTGGCGAAG TGGATCTGAC CGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTACGG 
CCACCTCACA AAGTAACGGA CTACTCCTCA TCCAGTGAGG AGTCGGGGAC GACGGATGAG 
GAGGACGACG ATGTGGAGCA GGAAGGGGCT GACGAGTCCA CCTCAGGACC AGAGGACACC 
AGAGCAGCGT CATCTCTGAA TTTGAGCAAT GGTGAAACGG AATCTGTGAA AACCATGATT 
GTCCATGATG ATGTAGAAAG TGAGCCGGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC 
GTCCGCCAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGCGGAAC AACAGTGACA 
TCTGTGGTGG GATTTTCCTG TGATGGGATG AGACCAGAAG CCATAAGGCA AGATCCTACC 
CGGAAAGGCT CAGTGGTCAA TGTGAATCCT ACCAACACTA GGCCACAGAG TGACACCCCG 
GAGATTCGTA AATACAAGAA GAGGTTTAAC TCTGAGATTC TGTGTGCTGC CTTATGGGGA 
GTGAATTTGC TAGTGGGTAC AGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG 
AAGGTCTATC CTCTTATCAA CCGAAGACGA TTTCAACAAA TGGACGTACT TGAGGGCTTG 
AATGTCTTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG 
TTAAGAAATA AAATACTTCA CAATGATCCA GAAGTTGAGA AGAAGCAGGG ATGGACAACC 
GTAGGGGATT TGGAAGGATG TGTACATTAT AAAGTTGTAA AATATGAAAG AATCAAATTT 
CTGGTGATTG CTTTGAAGAG TTCTGTGGAA GTCTATGCGT GGGCACCAAA GCCATATCAC 
AAATTTATGG CCTTTAAGTC ATTTGGAGAA TTGGTACATA AGCCATTACT GGTGGATCTC 
ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT 
GTTGATGTGG ATTCAGGATC AGTCTATGAC ATTTATCTAC CAACACATAT CCAGTGTAGC 
ATCAAACCCC ATGCAATCAT CATCCTCCCC AATACAGATG GAATGGAGCT TCTGGTGTGC 
TATGAAGATG AGGGGGTTTA TGTAAACACA TATGGAAGGA TCACCAAGGA TGTAGTTCTA 
CAGTGGGGAG AGATGCCTAC ATCAGTAGCA TATATTCGAT CCAATCAGAC AATGGGCTGG 
GGAGAGAAGG CCATAGAGAT CCGATCTGTG GAAACTGGTC ACTTGGATGG TGTGTTCATG 
CACAAAAGGG CTCAAAGACT AAAATTCTTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC 
TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT TATTTCATGA CCTTAGGCAG GACTTCTCTT 
CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860854.1
CDS	75..3806
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005365.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005365.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT 
GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC 
TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC 
TCCGGGGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC 
CTGGAAAATG CAGTGAAAAA ACCTGAAGAT AAAAAGGAAG TTTTCAGACC CCTCAAGCCT 
GCTGGCGAAG TGGATCTGAC CGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTACGG 
CCACCTCACA AAGTAACGGA CTACTCCTCA TCCAGTGAGG AGTCGGGGAC GACGGATGAG 
GAGGACGACG ATGTGGAGCA GGAAGGGGCT GACGAGTCCA CCTCAGGACC AGAGGACACC 
AGAGCAGCGT CATCTCTGAA TTTGAGCAAT GGTGAAACGG AATCTGTGAA AACCATGATT 
GTCCATGATG ATGTAGAAAG TGAGCCGGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC 
GTCCGCCAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGCGGAAC AACAGTGACA 
TCTGTGGTGG GATTTTCCTG TGATGGGATG AGACCAGAAG CCATAAGGCA AGATCCTACC 
CGGAAAGGCT CAGTGGTCAA TGTGAATCCT ACCAACACTA GGCCACAGAG TGACACCCCG 
GAGATTCGTA AATACAAGAA GAGGTTTAAC TCTGAGATTC TGTGTGCTGC CTTATGGGGA 
GTGAATTTGC TAGTGGGTAC AGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG 
AAGGTCTATC CTCTTATCAA CCGAAGACGA TTTCAACAAA TGGACGTACT TGAGGGCTTG 
AATGTCTTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG 
TTAAGAAATA AAATACTTCA CAATGATCCA GAAGTTGAGA AGAAGCAGGG ATGGACAACC 
GTAGGGGATT TGGAAGGATG TGTACATTAT AAAGTTGTAA AATATGAAAG AATCAAATTT 
CTGGTGATTG CTTTGAAGAG TTCTGTGGAA GTCTATGCGT GGGCACCAAA GCCATATCAC 
AAATTTATGG CCTTTAAGTC ATTTGGAGAA TTGGTACATA AGCCATTACT GGTGGATCTC 
ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT 
GTTGATGTGG ATTCAGGATC AGTCTATGAC ATTTATCTAC CAACACATAT CCAGTGTAGC 
ATCAAACCCC ATGCAATCAT CATCCTCCCC AATACAGATG GAATGGAGCT TCTGGTGTGC 
TATGAAGATG AGGGGGTTTA TGTAAACACA TATGGAAGGA TCACCAAGGA TGTAGTTCTA 
CAGTGGGGAG AGATGCCTAC ATCAGTAGCA TATATTCGAT CCAATCAGAC AATGGGCTGG 
GGAGAGAAGG CCATAGAGAT CCGATCTGTG GAAACTGGTC ACTTGGATGG TGTGTTCATG 
CACAAAAGGG CTCAAAGACT AAAATTCTTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC 
TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT TATTTCATGA CCTTAGGCAG GACTTCTCTT 
CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005366.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860855.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X29, mRNA.

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CloneID	OHu92789
Clone ID Related Accession (Same CDS sequence)	XM_017005366.1
Accession Version	XM_017005366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG 
TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC 
CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA 
AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA 
TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT 
AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA 
GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA 
TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA 
TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT 
GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA 
CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC 
TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA 
GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG 
AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG 
CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT 
AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA 
CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT 
GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG 
AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG 
TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG 
AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA 
AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC 
AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT 
GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA 
CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT 
GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT 
GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG 
AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC 
TATGACATTT ATCTACCAAC ACATGTAAGA AAGAACCCAC ACTCTATGAT CCAGTGTAGC 
ATCAAACCCC ATGCAATCAT CATCCTCCCC AATACAGATG GAATGGAGCT TCTGGTGTGC 
TATGAAGATG AGGGGGTTTA TGTAAACACA TATGGAAGGA TCACCAAGGA TGTAGTTCTA 
CAGTGGGGAG AGATGCCTAC ATCAGTAGCA TATATTCGAT CCAATCAGAC AATGGGCTGG 
GGAGAGAAGG CCATAGAGAT CCGATCTGTG GAAACTGGTC ACTTGGATGG TGTGTTCATG 
CACAAAAGGG CTCAAAGACT AAAATTCTTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC 
TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT TATTTCATGA CCTTAGGCAG GACTTCTCTT 
CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860855.1
CDS	75..3806
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005366.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005366.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG 
TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC 
CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA 
AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA 
TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT 
AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA 
GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA 
TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA 
TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT 
GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA 
CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC 
TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA 
GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG 
AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG 
CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT 
AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA 
CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT 
GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG 
AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG 
TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG 
AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA 
AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC 
AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT 
GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA 
CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT 
GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT 
GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG 
AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC 
TATGACATTT ATCTACCAAC ACATGTAAGA AAGAACCCAC ACTCTATGAT CCAGTGTAGC 
ATCAAACCCC ATGCAATCAT CATCCTCCCC AATACAGATG GAATGGAGCT TCTGGTGTGC 
TATGAAGATG AGGGGGTTTA TGTAAACACA TATGGAAGGA TCACCAAGGA TGTAGTTCTA 
CAGTGGGGAG AGATGCCTAC ATCAGTAGCA TATATTCGAT CCAATCAGAC AATGGGCTGG 
GGAGAGAAGG CCATAGAGAT CCGATCTGTG GAAACTGGTC ACTTGGATGG TGTGTTCATG 
CACAAAAGGG CTCAAAGACT AAAATTCTTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC 
TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT TATTTCATGA CCTTAGGCAG GACTTCTCTT 
CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005367.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860856.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X30, mRNA.

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CloneID	OHu92790
Clone ID Related Accession (Same CDS sequence)	XM_017005367.1
Accession Version	XM_017005367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG 
TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC 
CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA 
AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA 
TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT 
AGCCAGGCAG GACAGAGAAA CTCCACCAGT ATTGAGCCCA GGCTTCTGTG GGAGAGAGTG 
GAGAAGCTGG TGCCCAGACC TGGCAGTGGC AGCTCCTCAG GGTCCAGCAA CTCAGGATCC 
CAGCCCGGGT CTCACCCTGG GTCTCAGAGT GGCTCCGGGG AACGCTTCAG AGTGAGATCA 
TCATCCAAGT CTGAAGGCTC TCCATCTCAG CGCCTGGAAA ATGCAGTGAA AAAACCTGAA 
GATAAAAAGG AAGTTTTCAG ACCCCTCAAG CCTGCTGGCG AAGTGGATCT GACCGCACTG 
GCCAAAGAGC TTCGAGCAGT GGAAGATGTA CGGCCACCTC ACAAAGTAAC GGACTACTCC 
TCATCCAGTG AGGAGTCGGG GACGACGGAT GAGGAGGACG ACGATGTGGA GCAGGAAGGG 
GCTGACGAGT CCACCTCAGG ACCAGAGGAC ACCAGAGCAG CGTCATCTCT GAATTTGAGC 
AATGGTGAAA CGGAATCTGT GAAAACCATG ATTGTCCATG ATGATGTAGA AAGTGAGCCG 
GCCATGACCC CATCCAAGGA GGGCACTCTA ATCGTCCGCC AGACTCAGTC CGCTAGTAGC 
ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
CAGATTTCTC CATCTAGCGG AACAACAGTG ACATCTGTGG TGGGATTTTC CTGTGATGGG 
ATGAGACCAG AAGCCATAAG GCAAGATCCT ACCCGGAAAG GCTCAGTGGT CAATGTGAAT 
CCTACCAACA CTAGGCCACA GAGTGACACC CCGGAGATTC GTAAATACAA GAAGAGGTTT 
AACTCTGAGA TTCTGTGTGC TGCCTTATGG GGAGTGAATT TGCTAGTGGG TACAGAGAGT 
GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTCT ATCCTCTTAT CAACCGAAGA 
CGATTTCAAC AAATGGACGT ACTTGAGGGC TTGAATGTCT TGGTGACAAT ATCTGGCAAA 
AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAATGAT 
CCAGAAGTTG AGAAGAAGCA GGGATGGACA ACCGTAGGGG ATTTGGAAGG ATGTGTACAT 
TATAAAGTTG TAAAATATGA AAGAATCAAA TTTCTGGTGA TTGCTTTGAA GAGTTCTGTG 
GAAGTCTATG CGTGGGCACC AAAGCCATAT CACAAATTTA TGGCCTTTAA GTCATTTGGA 
GAATTGGTAC ATAAGCCATT ACTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGTTGAAA 
GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGATTCAGG ATCAGTCTAT 
GACATTTATC TACCAACACA TATCCAGTGT AGCATCAAAC CCCATGCAAT CATCATCCTC 
CCCAATACAG ATGGAATGGA GCTTCTGGTG TGCTATGAAG ATGAGGGGGT TTATGTAAAC 
ACATATGGAA GGATCACCAA GGATGTAGTT CTACAGTGGG GAGAGATGCC TACATCAGTA 
GCATATATTC GATCCAATCA GACAATGGGC TGGGGAGAGA AGGCCATAGA GATCCGATCT 
GTGGAAACTG GTCACTTGGA TGGTGTGTTC ATGCACAAAA GGGCTCAAAG ACTAAAATTC 
TTGTGTGAAC GCAATGACAA GGTGTTCTTT GCCTCTGTTC GGTCTGGTGG CAGCAGTCAG 
GTTTATTTCA TGACCTTAGG CAGGACTTCT CTTCTGAGCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860856.1
CDS	75..3779
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005367.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005367.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG 
TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC 
CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA 
AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA 
TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT 
AGCCAGGCAG GACAGAGAAA CTCCACCAGT ATTGAGCCCA GGCTTCTGTG GGAGAGAGTG 
GAGAAGCTGG TGCCCAGACC TGGCAGTGGC AGCTCCTCAG GGTCCAGCAA CTCAGGATCC 
CAGCCCGGGT CTCACCCTGG GTCTCAGAGT GGCTCCGGGG AACGCTTCAG AGTGAGATCA 
TCATCCAAGT CTGAAGGCTC TCCATCTCAG CGCCTGGAAA ATGCAGTGAA AAAACCTGAA 
GATAAAAAGG AAGTTTTCAG ACCCCTCAAG CCTGCTGGCG AAGTGGATCT GACCGCACTG 
GCCAAAGAGC TTCGAGCAGT GGAAGATGTA CGGCCACCTC ACAAAGTAAC GGACTACTCC 
TCATCCAGTG AGGAGTCGGG GACGACGGAT GAGGAGGACG ACGATGTGGA GCAGGAAGGG 
GCTGACGAGT CCACCTCAGG ACCAGAGGAC ACCAGAGCAG CGTCATCTCT GAATTTGAGC 
AATGGTGAAA CGGAATCTGT GAAAACCATG ATTGTCCATG ATGATGTAGA AAGTGAGCCG 
GCCATGACCC CATCCAAGGA GGGCACTCTA ATCGTCCGCC AGACTCAGTC CGCTAGTAGC 
ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
CAGATTTCTC CATCTAGCGG AACAACAGTG ACATCTGTGG TGGGATTTTC CTGTGATGGG 
ATGAGACCAG AAGCCATAAG GCAAGATCCT ACCCGGAAAG GCTCAGTGGT CAATGTGAAT 
CCTACCAACA CTAGGCCACA GAGTGACACC CCGGAGATTC GTAAATACAA GAAGAGGTTT 
AACTCTGAGA TTCTGTGTGC TGCCTTATGG GGAGTGAATT TGCTAGTGGG TACAGAGAGT 
GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTCT ATCCTCTTAT CAACCGAAGA 
CGATTTCAAC AAATGGACGT ACTTGAGGGC TTGAATGTCT TGGTGACAAT ATCTGGCAAA 
AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAATGAT 
CCAGAAGTTG AGAAGAAGCA GGGATGGACA ACCGTAGGGG ATTTGGAAGG ATGTGTACAT 
TATAAAGTTG TAAAATATGA AAGAATCAAA TTTCTGGTGA TTGCTTTGAA GAGTTCTGTG 
GAAGTCTATG CGTGGGCACC AAAGCCATAT CACAAATTTA TGGCCTTTAA GTCATTTGGA 
GAATTGGTAC ATAAGCCATT ACTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGTTGAAA 
GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGATTCAGG ATCAGTCTAT 
GACATTTATC TACCAACACA TATCCAGTGT AGCATCAAAC CCCATGCAAT CATCATCCTC 
CCCAATACAG ATGGAATGGA GCTTCTGGTG TGCTATGAAG ATGAGGGGGT TTATGTAAAC 
ACATATGGAA GGATCACCAA GGATGTAGTT CTACAGTGGG GAGAGATGCC TACATCAGTA 
GCATATATTC GATCCAATCA GACAATGGGC TGGGGAGAGA AGGCCATAGA GATCCGATCT 
GTGGAAACTG GTCACTTGGA TGGTGTGTTC ATGCACAAAA GGGCTCAAAG ACTAAAATTC 
TTGTGTGAAC GCAATGACAA GGTGTTCTTT GCCTCTGTTC GGTCTGGTGG CAGCAGTCAG 
GTTTATTTCA TGACCTTAGG CAGGACTTCT CTTCTGAGCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005368.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860857.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X34, mRNA.

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CloneID	OHu92791
Clone ID Related Accession (Same CDS sequence)	XM_017005368.1
Accession Version	XM_017005368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG TATTGAGCCC AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG 
GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA GGGTCCAGCA ACTCAGGATC CCAGCCCGGG 
TCTCACCCTG GGTCTCAGAG TGGCTCCGGG GAACGCTTCA GAGTGAGATC ATCATCCAAG 
TCTGAAGGCT CTCCATCTCA GCGCCTGGAA AATGCAGTGA AAAAACCTGA AGATAAAAAG 
GAAGTTTTCA GACCCCTCAA GCCTGCTGGC GAAGTGGATC TGACCGCACT GGCCAAAGAG 
CTTCGAGCAG TGGAAGATGT ACGGCCACCT CACAAAGTAA CGGACTACTC CTCATCCAGT 
GAGGAGTCGG GGACGACGGA TGAGGAGGAC GACGATGTGG AGCAGGAAGG GGCTGACGAG 
TCCACCTCAG GACCAGAGGA CACCAGAGCA GCGTCATCTC TGAATTTGAG CAATGGTGAA 
ACGGAATCTG TGAAAACCAT GATTGTCCAT GATGATGTAG AAAGTGAGCC GGCCATGACC 
CCATCCAAGG AGGGCACTCT AATCGTCCGC CAGACTCAGT CCGCTAGTAG CACACTCCAG 
AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
CCATCTAGCG GAACAACAGT GACATCTGTG GTGGGATTTT CCTGTGATGG GATGAGACCA 
GAAGCCATAA GGCAAGATCC TACCCGGAAA GGCTCAGTGG TCAATGTGAA TCCTACCAAC 
ACTAGGCCAC AGAGTGACAC CCCGGAGATT CGTAAATACA AGAAGAGGTT TAACTCTGAG 
ATTCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTGG GTACAGAGAG TGGCCTGATG 
CTGCTGGACA GAAGTGGCCA AGGGAAGGTC TATCCTCTTA TCAACCGAAG ACGATTTCAA 
CAAATGGACG TACTTGAGGG CTTGAATGTC TTGGTGACAA TATCTGGCAA AAAGGATAAG 
TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAATGA TCCAGAAGTT 
GAGAAGAAGC AGGGATGGAC AACCGTAGGG GATTTGGAAG GATGTGTACA TTATAAAGTT 
GTAAAATATG AAAGAATCAA ATTTCTGGTG ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT 
GCGTGGGCAC CAAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAATTGGTA 
CATAAGCCAT TACTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGTTGAA AGTGATCTAT 
GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCAG GATCAGTCTA TGACATTTAT 
CTACCAACAC ATATCCAGTG TAGCATCAAA CCCCATGCAA TCATCATCCT CCCCAATACA 
GATGGAATGG AGCTTCTGGT GTGCTATGAA GATGAGGGGG TTTATGTAAA CACATATGGA 
AGGATCACCA AGGATGTAGT TCTACAGTGG GGAGAGATGC CTACATCAGT AGCATATATT 
CGATCCAATC AGACAATGGG CTGGGGAGAG AAGGCCATAG AGATCCGATC TGTGGAAACT 
GGTCACTTGG ATGGTGTGTT CATGCACAAA AGGGCTCAAA GACTAAAATT CTTGTGTGAA 
CGCAATGACA AGGTGTTCTT TGCCTCTGTT CGGTCTGGTG GCAGCAGTCA GGTTTATTTC 
ATGACCTTAG GCAGGACTTC TCTTCTGAGC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860857.1
CDS	75..3710
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005368.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005368.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG TATTGAGCCC AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG 
GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA GGGTCCAGCA ACTCAGGATC CCAGCCCGGG 
TCTCACCCTG GGTCTCAGAG TGGCTCCGGG GAACGCTTCA GAGTGAGATC ATCATCCAAG 
TCTGAAGGCT CTCCATCTCA GCGCCTGGAA AATGCAGTGA AAAAACCTGA AGATAAAAAG 
GAAGTTTTCA GACCCCTCAA GCCTGCTGGC GAAGTGGATC TGACCGCACT GGCCAAAGAG 
CTTCGAGCAG TGGAAGATGT ACGGCCACCT CACAAAGTAA CGGACTACTC CTCATCCAGT 
GAGGAGTCGG GGACGACGGA TGAGGAGGAC GACGATGTGG AGCAGGAAGG GGCTGACGAG 
TCCACCTCAG GACCAGAGGA CACCAGAGCA GCGTCATCTC TGAATTTGAG CAATGGTGAA 
ACGGAATCTG TGAAAACCAT GATTGTCCAT GATGATGTAG AAAGTGAGCC GGCCATGACC 
CCATCCAAGG AGGGCACTCT AATCGTCCGC CAGACTCAGT CCGCTAGTAG CACACTCCAG 
AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
CCATCTAGCG GAACAACAGT GACATCTGTG GTGGGATTTT CCTGTGATGG GATGAGACCA 
GAAGCCATAA GGCAAGATCC TACCCGGAAA GGCTCAGTGG TCAATGTGAA TCCTACCAAC 
ACTAGGCCAC AGAGTGACAC CCCGGAGATT CGTAAATACA AGAAGAGGTT TAACTCTGAG 
ATTCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTGG GTACAGAGAG TGGCCTGATG 
CTGCTGGACA GAAGTGGCCA AGGGAAGGTC TATCCTCTTA TCAACCGAAG ACGATTTCAA 
CAAATGGACG TACTTGAGGG CTTGAATGTC TTGGTGACAA TATCTGGCAA AAAGGATAAG 
TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAATGA TCCAGAAGTT 
GAGAAGAAGC AGGGATGGAC AACCGTAGGG GATTTGGAAG GATGTGTACA TTATAAAGTT 
GTAAAATATG AAAGAATCAA ATTTCTGGTG ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT 
GCGTGGGCAC CAAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAATTGGTA 
CATAAGCCAT TACTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGTTGAA AGTGATCTAT 
GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCAG GATCAGTCTA TGACATTTAT 
CTACCAACAC ATATCCAGTG TAGCATCAAA CCCCATGCAA TCATCATCCT CCCCAATACA 
GATGGAATGG AGCTTCTGGT GTGCTATGAA GATGAGGGGG TTTATGTAAA CACATATGGA 
AGGATCACCA AGGATGTAGT TCTACAGTGG GGAGAGATGC CTACATCAGT AGCATATATT 
CGATCCAATC AGACAATGGG CTGGGGAGAG AAGGCCATAG AGATCCGATC TGTGGAAACT 
GGTCACTTGG ATGGTGTGTT CATGCACAAA AGGGCTCAAA GACTAAAATT CTTGTGTGAA 
CGCAATGACA AGGTGTTCTT TGCCTCTGTT CGGTCTGGTG GCAGCAGTCA GGTTTATTTC 
ATGACCTTAG GCAGGACTTC TCTTCTGAGC TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005369.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860858.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X36, mRNA.

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CloneID	OHu92792
Clone ID Related Accession (Same CDS sequence)	XM_017005369.1
Accession Version	XM_017005369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X36
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA 
CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT 
GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC 
TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC 
AGACCCCTCA AGCCTGCTGG CGAAGTGGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA 
GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG 
GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA 
GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT 
GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG 
GAGGGCACTC TAATCGTCCG CCAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA 
TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGATTAC TACAGATTTC TCCATCTAGC 
GGAACAACAG TGACATCTGT GGTGGGATTT TCCTGTGATG GGATGAGACC AGAAGCCATA 
AGGCAAGATC CTACCCGGAA AGGCTCAGTG GTCAATGTGA ATCCTACCAA CACTAGGCCA 
CAGAGTGACA CCCCGGAGAT TCGTAAATAC AAGAAGAGGT TTAACTCTGA GATTCTGTGT 
GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG GGTACAGAGA GTGGCCTGAT GCTGCTGGAC 
AGAAGTGGCC AAGGGAAGGT CTATCCTCTT ATCAACCGAA GACGATTTCA ACAAATGGAC 
GTACTTGAGG GCTTGAATGT CTTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC 
TACTATTTGT CCTGGTTAAG AAATAAAATA CTTCACAATG ATCCAGAAGT TGAGAAGAAG 
CAGGGATGGA CAACCGTAGG GGATTTGGAA GGATGTGTAC ATTATAAAGT TGTAAAATAT 
GAAAGAATCA AATTTCTGGT GATTGCTTTG AAGAGTTCTG TGGAAGTCTA TGCGTGGGCA 
CCAAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAATTGGT ACATAAGCCA 
TTACTGGTGG ATCTCACTGT TGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT 
GCTGGATTCC ATGCTGTTGA TGTGGATTCA GGATCAGTCT ATGACATTTA TCTACCAACA 
CATGTAAGAA AGAACCCACA CTCTATGATC CAGTGTAGCA TCAAACCCCA TGCAATCATC 
ATCCTCCCCA ATACAGATGG AATGGAGCTT CTGGTGTGCT ATGAAGATGA GGGGGTTTAT 
GTAAACACAT ATGGAAGGAT CACCAAGGAT GTAGTTCTAC AGTGGGGAGA GATGCCTACA 
TCAGTAGCAT ATATTCGATC CAATCAGACA ATGGGCTGGG GAGAGAAGGC CATAGAGATC 
CGATCTGTGG AAACTGGTCA CTTGGATGGT GTGTTCATGC ACAAAAGGGC TCAAAGACTA 
AAATTCTTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC 
AGTCAGGTTT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860858.1
CDS	75..3665
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005369.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005369.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA 
CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT 
GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC 
TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC 
AGACCCCTCA AGCCTGCTGG CGAAGTGGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA 
GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG 
GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA 
GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT 
GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG 
GAGGGCACTC TAATCGTCCG CCAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA 
TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGATTAC TACAGATTTC TCCATCTAGC 
GGAACAACAG TGACATCTGT GGTGGGATTT TCCTGTGATG GGATGAGACC AGAAGCCATA 
AGGCAAGATC CTACCCGGAA AGGCTCAGTG GTCAATGTGA ATCCTACCAA CACTAGGCCA 
CAGAGTGACA CCCCGGAGAT TCGTAAATAC AAGAAGAGGT TTAACTCTGA GATTCTGTGT 
GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG GGTACAGAGA GTGGCCTGAT GCTGCTGGAC 
AGAAGTGGCC AAGGGAAGGT CTATCCTCTT ATCAACCGAA GACGATTTCA ACAAATGGAC 
GTACTTGAGG GCTTGAATGT CTTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC 
TACTATTTGT CCTGGTTAAG AAATAAAATA CTTCACAATG ATCCAGAAGT TGAGAAGAAG 
CAGGGATGGA CAACCGTAGG GGATTTGGAA GGATGTGTAC ATTATAAAGT TGTAAAATAT 
GAAAGAATCA AATTTCTGGT GATTGCTTTG AAGAGTTCTG TGGAAGTCTA TGCGTGGGCA 
CCAAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAATTGGT ACATAAGCCA 
TTACTGGTGG ATCTCACTGT TGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT 
GCTGGATTCC ATGCTGTTGA TGTGGATTCA GGATCAGTCT ATGACATTTA TCTACCAACA 
CATGTAAGAA AGAACCCACA CTCTATGATC CAGTGTAGCA TCAAACCCCA TGCAATCATC 
ATCCTCCCCA ATACAGATGG AATGGAGCTT CTGGTGTGCT ATGAAGATGA GGGGGTTTAT 
GTAAACACAT ATGGAAGGAT CACCAAGGAT GTAGTTCTAC AGTGGGGAGA GATGCCTACA 
TCAGTAGCAT ATATTCGATC CAATCAGACA ATGGGCTGGG GAGAGAAGGC CATAGAGATC 
CGATCTGTGG AAACTGGTCA CTTGGATGGT GTGTTCATGC ACAAAAGGGC TCAAAGACTA 
AAATTCTTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC 
AGTCAGGTTT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005370.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860859.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X38, mRNA.

Price & Availability↑

CloneID	OHu92793
Clone ID Related Accession (Same CDS sequence)	XM_017005370.1
Accession Version	XM_017005370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X38
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA 
CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT 
GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC 
TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC 
AGACCCCTCA AGCCTGCTGG CGAAGTGGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA 
GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG 
GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA 
GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT 
GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG 
GAGGGCACTC TAATCGTCCG CCAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA 
TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGATTAC TACAGATTTC TCCATCTAGC 
GGAACAACAG TGACATCTGT GGTGGGATTT TCCTGTGATG GGATGAGACC AGAAGCCATA 
AGGCAAGATC CTACCCGGAA AGGCTCAGTG GTCAATGTGA ATCCTACCAA CACTAGGCCA 
CAGAGTGACA CCCCGGAGAT TCGTAAATAC AAGAAGAGGT TTAACTCTGA GATTCTGTGT 
GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG GGTACAGAGA GTGGCCTGAT GCTGCTGGAC 
AGAAGTGGCC AAGGGAAGGT CTATCCTCTT ATCAACCGAA GACGATTTCA ACAAATGGAC 
GTACTTGAGG GCTTGAATGT CTTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC 
TACTATTTGT CCTGGTTAAG AAATAAAATA CTTCACAATG ATCCAGAAGT TGAGAAGAAG 
CAGGGATGGA CAACCGTAGG GGATTTGGAA GGATGTGTAC ATTATAAAGT TGTAAAATAT 
GAAAGAATCA AATTTCTGGT GATTGCTTTG AAGAGTTCTG TGGAAGTCTA TGCGTGGGCA 
CCAAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAATTGGT ACATAAGCCA 
TTACTGGTGG ATCTCACTGT TGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT 
GCTGGATTCC ATGCTGTTGA TGTGGATTCA GGATCAGTCT ATGACATTTA TCTACCAACA 
CATATCCAGT GTAGCATCAA ACCCCATGCA ATCATCATCC TCCCCAATAC AGATGGAATG 
GAGCTTCTGG TGTGCTATGA AGATGAGGGG GTTTATGTAA ACACATATGG AAGGATCACC 
AAGGATGTAG TTCTACAGTG GGGAGAGATG CCTACATCAG TAGCATATAT TCGATCCAAT 
CAGACAATGG GCTGGGGAGA GAAGGCCATA GAGATCCGAT CTGTGGAAAC TGGTCACTTG 
GATGGTGTGT TCATGCACAA AAGGGCTCAA AGACTAAAAT TCTTGTGTGA ACGCAATGAC 
AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT GGCAGCAGTC AGGTTTATTT CATGACCTTA 
GGCAGGACTT CTCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860859.1
CDS	75..3641
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005370.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005370.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA 
CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT 
GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC 
TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC 
AGACCCCTCA AGCCTGCTGG CGAAGTGGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA 
GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG 
GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA 
GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT 
GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG 
GAGGGCACTC TAATCGTCCG CCAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA 
TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGATTAC TACAGATTTC TCCATCTAGC 
GGAACAACAG TGACATCTGT GGTGGGATTT TCCTGTGATG GGATGAGACC AGAAGCCATA 
AGGCAAGATC CTACCCGGAA AGGCTCAGTG GTCAATGTGA ATCCTACCAA CACTAGGCCA 
CAGAGTGACA CCCCGGAGAT TCGTAAATAC AAGAAGAGGT TTAACTCTGA GATTCTGTGT 
GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG GGTACAGAGA GTGGCCTGAT GCTGCTGGAC 
AGAAGTGGCC AAGGGAAGGT CTATCCTCTT ATCAACCGAA GACGATTTCA ACAAATGGAC 
GTACTTGAGG GCTTGAATGT CTTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC 
TACTATTTGT CCTGGTTAAG AAATAAAATA CTTCACAATG ATCCAGAAGT TGAGAAGAAG 
CAGGGATGGA CAACCGTAGG GGATTTGGAA GGATGTGTAC ATTATAAAGT TGTAAAATAT 
GAAAGAATCA AATTTCTGGT GATTGCTTTG AAGAGTTCTG TGGAAGTCTA TGCGTGGGCA 
CCAAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAATTGGT ACATAAGCCA 
TTACTGGTGG ATCTCACTGT TGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT 
GCTGGATTCC ATGCTGTTGA TGTGGATTCA GGATCAGTCT ATGACATTTA TCTACCAACA 
CATATCCAGT GTAGCATCAA ACCCCATGCA ATCATCATCC TCCCCAATAC AGATGGAATG 
GAGCTTCTGG TGTGCTATGA AGATGAGGGG GTTTATGTAA ACACATATGG AAGGATCACC 
AAGGATGTAG TTCTACAGTG GGGAGAGATG CCTACATCAG TAGCATATAT TCGATCCAAT 
CAGACAATGG GCTGGGGAGA GAAGGCCATA GAGATCCGAT CTGTGGAAAC TGGTCACTTG 
GATGGTGTGT TCATGCACAA AAGGGCTCAA AGACTAAAAT TCTTGTGTGA ACGCAATGAC 
AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT GGCAGCAGTC AGGTTTATTT CATGACCTTA 
GGCAGGACTT CTCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005371.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860860.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X39, mRNA.

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CloneID	OHu92794
Clone ID Related Accession (Same CDS sequence)	XM_017005371.1
Accession Version	XM_017005371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X39
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC 
AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC 
CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA 
GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT 
TTCAGACCCC TCAAGCCTGC TGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA 
GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG 
ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA 
GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA 
ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC 
ACTCTAATCG TCCGCCAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC 
TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA 
ACAGTGACAT CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA 
GATCCTACCC GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT 
GACACCCCGG AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC 
TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT 
GGCCAAGGGA AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT 
GAGGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT 
TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA 
TGGACAACCG TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA 
ATCAAATTTC TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG 
CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG 
GTGGATCTCA CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA 
TTCCATGCTG TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATATC 
CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT 
CTGGTGTGCT ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT 
GTAGTTCTAC AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA 
ATGGGCTGGG GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT 
GTGTTCATGC ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG 
TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG 
ACTTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860860.1
CDS	75..3635
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005371.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005371.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC 
AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC 
CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA 
GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT 
TTCAGACCCC TCAAGCCTGC TGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA 
GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG 
ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA 
GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA 
ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC 
ACTCTAATCG TCCGCCAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC 
TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA 
ACAGTGACAT CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA 
GATCCTACCC GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT 
GACACCCCGG AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC 
TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT 
GGCCAAGGGA AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT 
GAGGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT 
TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA 
TGGACAACCG TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA 
ATCAAATTTC TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG 
CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG 
GTGGATCTCA CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA 
TTCCATGCTG TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATATC 
CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT 
CTGGTGTGCT ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT 
GTAGTTCTAC AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA 
ATGGGCTGGG GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT 
GTGTTCATGC ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG 
TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG 
ACTTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005372.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860861.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X41, mRNA.

Price & Availability↑

CloneID	OHu92795
Clone ID Related Accession (Same CDS sequence)	XM_017005372.1
Accession Version	XM_017005372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X41
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG 
AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT 
CTGACCGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TACGGCCACC TCACAAAGTA 
ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG 
GAGCAGGAAG GGGCTGACGA GTCCACCTCA GGACCAGAGG ACACCAGAGC AGCGTCATCT 
CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA 
GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGACTCAG 
TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
CCCAGATTAC TACAGATTTC TCCATCTAGC GGAACAACAG TGACATCTGT GGTGGGATTT 
TCCTGTGATG GGATGAGACC AGAAGCCATA AGGCAAGATC CTACCCGGAA AGGCTCAGTG 
GTCAATGTGA ATCCTACCAA CACTAGGCCA CAGAGTGACA CCCCGGAGAT TCGTAAATAC 
AAGAAGAGGT TTAACTCTGA GATTCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG 
GGTACAGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT CTATCCTCTT 
ATCAACCGAA GACGATTTCA ACAAATGGAC GTACTTGAGG GCTTGAATGT CTTGGTGACA 
ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA 
CTTCACAATG ATCCAGAAGT TGAGAAGAAG CAGGGATGGA CAACCGTAGG GGATTTGGAA 
GGATGTGTAC ATTATAAAGT TGTAAAATAT GAAAGAATCA AATTTCTGGT GATTGCTTTG 
AAGAGTTCTG TGGAAGTCTA TGCGTGGGCA CCAAAGCCAT ATCACAAATT TATGGCCTTT 
AAGTCATTTG GAGAATTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT TGAGGAAGGC 
CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCA 
GGATCAGTCT ATGACATTTA TCTACCAACA CATGTAAGAA AGAACCCACA CTCTATGATC 
CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT 
CTGGTGTGCT ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT 
GTAGTTCTAC AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA 
ATGGGCTGGG GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT 
GTGTTCATGC ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG 
TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG 
ACTTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860861.1
CDS	75..3575
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005372.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X41, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005372.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG 
AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT 
CTGACCGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TACGGCCACC TCACAAAGTA 
ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG 
GAGCAGGAAG GGGCTGACGA GTCCACCTCA GGACCAGAGG ACACCAGAGC AGCGTCATCT 
CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA 
GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGACTCAG 
TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
CCCAGATTAC TACAGATTTC TCCATCTAGC GGAACAACAG TGACATCTGT GGTGGGATTT 
TCCTGTGATG GGATGAGACC AGAAGCCATA AGGCAAGATC CTACCCGGAA AGGCTCAGTG 
GTCAATGTGA ATCCTACCAA CACTAGGCCA CAGAGTGACA CCCCGGAGAT TCGTAAATAC 
AAGAAGAGGT TTAACTCTGA GATTCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG 
GGTACAGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT CTATCCTCTT 
ATCAACCGAA GACGATTTCA ACAAATGGAC GTACTTGAGG GCTTGAATGT CTTGGTGACA 
ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA 
CTTCACAATG ATCCAGAAGT TGAGAAGAAG CAGGGATGGA CAACCGTAGG GGATTTGGAA 
GGATGTGTAC ATTATAAAGT TGTAAAATAT GAAAGAATCA AATTTCTGGT GATTGCTTTG 
AAGAGTTCTG TGGAAGTCTA TGCGTGGGCA CCAAAGCCAT ATCACAAATT TATGGCCTTT 
AAGTCATTTG GAGAATTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT TGAGGAAGGC 
CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCA 
GGATCAGTCT ATGACATTTA TCTACCAACA CATGTAAGAA AGAACCCACA CTCTATGATC 
CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT 
CTGGTGTGCT ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT 
GTAGTTCTAC AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA 
ATGGGCTGGG GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT 
GTGTTCATGC ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG 
TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG 
ACTTCTCTTC TGAGCTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005373.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860862.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X42, mRNA.

Price & Availability↑

CloneID	OHu92796
Clone ID Related Accession (Same CDS sequence)	XM_017005373.1
Accession Version	XM_017005373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X42
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG 
AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGA TCTGACCGCA 
CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC 
TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA 
GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG 
AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG 
CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT 
AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA 
CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT 
GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG 
AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG 
TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG 
AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA 
AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC 
AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT 
GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA 
CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT 
GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT 
GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG 
AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC 
TATGACATTT ATCTACCAAC ACATGTAAGA AAGAACCCAC ACTCTATGAT CCAGTGTAGC 
ATCAAACCCC ATGCAATCAT CATCCTCCCC AATACAGATG GAATGGAGCT TCTGGTGTGC 
TATGAAGATG AGGGGGTTTA TGTAAACACA TATGGAAGGA TCACCAAGGA TGTAGTTCTA 
CAGTGGGGAG AGATGCCTAC ATCAGTAGCA TATATTCGAT CCAATCAGAC AATGGGCTGG 
GGAGAGAAGG CCATAGAGAT CCGATCTGTG GAAACTGGTC ACTTGGATGG TGTGTTCATG 
CACAAAAGGG CTCAAAGACT AAAATTCTTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC 
TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT TATTTCATGA CCTTAGGCAG GACTTCTCTT 
CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860862.1
CDS	75..3566
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005373.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X42, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005373.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG 
AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGA TCTGACCGCA 
CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC 
TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA 
GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG 
AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG 
CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT 
AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA 
CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT 
GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG 
AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG 
TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG 
AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA 
AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC 
AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT 
GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA 
CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT 
GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT 
GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG 
AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC 
TATGACATTT ATCTACCAAC ACATGTAAGA AAGAACCCAC ACTCTATGAT CCAGTGTAGC 
ATCAAACCCC ATGCAATCAT CATCCTCCCC AATACAGATG GAATGGAGCT TCTGGTGTGC 
TATGAAGATG AGGGGGTTTA TGTAAACACA TATGGAAGGA TCACCAAGGA TGTAGTTCTA 
CAGTGGGGAG AGATGCCTAC ATCAGTAGCA TATATTCGAT CCAATCAGAC AATGGGCTGG 
GGAGAGAAGG CCATAGAGAT CCGATCTGTG GAAACTGGTC ACTTGGATGG TGTGTTCATG 
CACAAAAGGG CTCAAAGACT AAAATTCTTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC 
TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT TATTTCATGA CCTTAGGCAG GACTTCTCTT 
CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005374.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860863.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X43, mRNA.

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CloneID	OHu92797
Clone ID Related Accession (Same CDS sequence)	XM_017005374.1
Accession Version	XM_017005374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X43
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG 
AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT 
CTGACCGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TACGGCCACC TCACAAAGTA 
ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG 
GAGCAGGAAG GGGCTGACGA GTCCACCTCA GGACCAGAGG ACACCAGAGC AGCGTCATCT 
CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA 
GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGACTCAG 
TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
CCCAGATTAC TACAGATTTC TCCATCTAGC GGAACAACAG TGACATCTGT GGTGGGATTT 
TCCTGTGATG GGATGAGACC AGAAGCCATA AGGCAAGATC CTACCCGGAA AGGCTCAGTG 
GTCAATGTGA ATCCTACCAA CACTAGGCCA CAGAGTGACA CCCCGGAGAT TCGTAAATAC 
AAGAAGAGGT TTAACTCTGA GATTCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG 
GGTACAGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT CTATCCTCTT 
ATCAACCGAA GACGATTTCA ACAAATGGAC GTACTTGAGG GCTTGAATGT CTTGGTGACA 
ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA 
CTTCACAATG ATCCAGAAGT TGAGAAGAAG CAGGGATGGA CAACCGTAGG GGATTTGGAA 
GGATGTGTAC ATTATAAAGT TGTAAAATAT GAAAGAATCA AATTTCTGGT GATTGCTTTG 
AAGAGTTCTG TGGAAGTCTA TGCGTGGGCA CCAAAGCCAT ATCACAAATT TATGGCCTTT 
AAGTCATTTG GAGAATTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT TGAGGAAGGC 
CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCA 
GGATCAGTCT ATGACATTTA TCTACCAACA CATATCCAGT GTAGCATCAA ACCCCATGCA 
ATCATCATCC TCCCCAATAC AGATGGAATG GAGCTTCTGG TGTGCTATGA AGATGAGGGG 
GTTTATGTAA ACACATATGG AAGGATCACC AAGGATGTAG TTCTACAGTG GGGAGAGATG 
CCTACATCAG TAGCATATAT TCGATCCAAT CAGACAATGG GCTGGGGAGA GAAGGCCATA 
GAGATCCGAT CTGTGGAAAC TGGTCACTTG GATGGTGTGT TCATGCACAA AAGGGCTCAA 
AGACTAAAAT TCTTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT 
GGCAGCAGTC AGGTTTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860863.1
CDS	75..3551
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005374.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X43, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005374.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG 
AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT 
CTGACCGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TACGGCCACC TCACAAAGTA 
ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG 
GAGCAGGAAG GGGCTGACGA GTCCACCTCA GGACCAGAGG ACACCAGAGC AGCGTCATCT 
CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA 
GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGACTCAG 
TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
CCCAGATTAC TACAGATTTC TCCATCTAGC GGAACAACAG TGACATCTGT GGTGGGATTT 
TCCTGTGATG GGATGAGACC AGAAGCCATA AGGCAAGATC CTACCCGGAA AGGCTCAGTG 
GTCAATGTGA ATCCTACCAA CACTAGGCCA CAGAGTGACA CCCCGGAGAT TCGTAAATAC 
AAGAAGAGGT TTAACTCTGA GATTCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG 
GGTACAGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT CTATCCTCTT 
ATCAACCGAA GACGATTTCA ACAAATGGAC GTACTTGAGG GCTTGAATGT CTTGGTGACA 
ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA 
CTTCACAATG ATCCAGAAGT TGAGAAGAAG CAGGGATGGA CAACCGTAGG GGATTTGGAA 
GGATGTGTAC ATTATAAAGT TGTAAAATAT GAAAGAATCA AATTTCTGGT GATTGCTTTG 
AAGAGTTCTG TGGAAGTCTA TGCGTGGGCA CCAAAGCCAT ATCACAAATT TATGGCCTTT 
AAGTCATTTG GAGAATTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT TGAGGAAGGC 
CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCA 
GGATCAGTCT ATGACATTTA TCTACCAACA CATATCCAGT GTAGCATCAA ACCCCATGCA 
ATCATCATCC TCCCCAATAC AGATGGAATG GAGCTTCTGG TGTGCTATGA AGATGAGGGG 
GTTTATGTAA ACACATATGG AAGGATCACC AAGGATGTAG TTCTACAGTG GGGAGAGATG 
CCTACATCAG TAGCATATAT TCGATCCAAT CAGACAATGG GCTGGGGAGA GAAGGCCATA 
GAGATCCGAT CTGTGGAAAC TGGTCACTTG GATGGTGTGT TCATGCACAA AAGGGCTCAA 
AGACTAAAAT TCTTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT 
GGCAGCAGTC AGGTTTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_017005375.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860864.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X44, mRNA.

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CloneID	OHu92798
Clone ID Related Accession (Same CDS sequence)	XM_017005375.1
Accession Version	XM_017005375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X44
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG 
AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGA TCTGACCGCA 
CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC 
TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA 
GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG 
AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG 
CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT 
AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA 
CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT 
GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG 
AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG 
TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG 
AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA 
AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC 
AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT 
GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA 
CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT 
GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT 
GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG 
AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC 
TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA AACCCCATGC AATCATCATC 
CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA 
AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA 
GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA 
TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA 
TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT 
CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860864.1
CDS	75..3542
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_017005375.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X44, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005375.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG 
AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGA TCTGACCGCA 
CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC 
TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA 
GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG 
AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG 
CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT 
AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA 
CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT 
GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG 
AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG 
TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG 
AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA 
AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC 
AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT 
GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA 
CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT 
GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT 
GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG 
AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC 
TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA AACCCCATGC AATCATCATC 
CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA 
AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA 
GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA 
TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA 
TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT 
CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( NM_145687.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_663720.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 3, mRNA.

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CloneID	OHu20375
Clone ID Related Accession (Same CDS sequence)	NM_145687.3 , NM_145687.4
Accession Version	NM_145687.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-29
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB086475.1, AB014587.1, AC005035.2 and BU625485.1. On Jun 14, 2011 this sequence version replaced NM_145687.2. Transcript Variant: This variant (3) lacks an alternate in-frame exon but includes a different in-frame exon in the central coding region, compared to variant 2. The encoded isoform (3) is shorter than isoform 2. The 5' UTR of this variant is incomplete due to a lack of 5'-complete transcripts representing this variant, and the presence of splicing ambiguity in the 5' UTR. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU794634.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA 
AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA 
GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC 
AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC 
GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT 
GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG 
ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGACTC AGTCCGCTAG TAGCACACTC 
CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA 
CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC 
AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT 
GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG 
ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT 
CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT 
AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA 
GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA 
GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC 
TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG 
GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC 
TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT 
TATCTACCAA CACATATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT 
ACAGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT 
GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACATC AGTAGCATAT 
ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA 
ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT 
GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT 
TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663720.1
CDS	6..3644
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	NM_145687.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145687.3

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA 
AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA 
GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC 
AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC 
GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT 
GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG 
ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGACTC AGTCCGCTAG TAGCACACTC 
CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA 
CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC 
AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT 
GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG 
ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT 
CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT 
AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA 
GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA 
GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC 
TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG 
GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC 
TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT 
TATCTACCAA CACATATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT 
ACAGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT 
GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACATC AGTAGCATAT 
ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA 
ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT 
GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT 
TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( NM_145687.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_663720.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu20375
Clone ID Related Accession (Same CDS sequence)	NM_145687.3 , NM_145687.4
Accession Version	NM_145687.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007005.3, DB086475.1, AB014587.1, AC005035.2 and BU625485.1. On Jun 2, 2019 this sequence version replaced NM_145687.3. Transcript Variant: This variant (3) lacks an alternate in-frame exon but includes a different in-frame exon in the central coding region, compared to variant 2. The encoded isoform (3) is shorter than isoform 2. The 5' UTR of this variant is incomplete due to a lack of 5'-complete transcripts representing this variant, and the presence of splicing ambiguity in the 5' UTR. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU794634.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA 
AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA 
GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC 
AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC 
GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT 
GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG 
ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGACTC AGTCCGCTAG TAGCACACTC 
CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA 
CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC 
AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT 
GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG 
ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT 
CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT 
AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA 
GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA 
GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC 
TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG 
GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC 
TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT 
TATCTACCAA CACATATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT 
ACAGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT 
GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACATC AGTAGCATAT 
ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA 
ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT 
GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT 
TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663720.1
CDS	375..4013
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	NM_145687.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145687.4

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA 
AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA 
GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC 
AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC 
GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT 
GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG 
ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGACTC AGTCCGCTAG TAGCACACTC 
CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA 
CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC 
AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT 
GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG 
ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT 
CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT 
AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA 
GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA 
GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC 
TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG 
GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC 
TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT 
TATCTACCAA CACATATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT 
ACAGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT 
GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACATC AGTAGCATAT 
ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA 
ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT 
GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT 
TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( NM_145686.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_663719.2 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu20734
Clone ID Related Accession (Same CDS sequence)	NM_145686.3
Accession Version	NM_145686.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002278.1, AC007005.3, BC064691.1, AC005035.2 and BU625485.1. On Jun 14, 2011 this sequence version replaced NM_145686.2. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064691.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA 
AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC 
TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT 
GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA 
CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
ATTTCTCCAT CTAGCGGAAC AACAGTGACA TCTGTGGTGG GATTTTCCTG TGATGGGATG 
AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT 
ACCAACACTA GGCCACAGAG TGACACCCCG GAGATTCGTA AATACAAGAA GAGGTTTAAC 
TCTGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC 
CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTTATCAA CCGAAGACGA 
TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG 
GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA 
GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT 
AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAGAG TTCTGTGGAA 
GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA 
TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG 
ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCAGGATC AGTCTATGAC 
ATTTATCTAC CAACACATAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC 
AATACAGATG GAATGGAGCT TCTGGTGTGC TATGAAGATG AGGGGGTTTA TGTAAACACA 
TATGGAAGGA TCACCAAGGA TGTAGTTCTA CAGTGGGGAG AGATGCCTAC ATCAGTAGCA 
TATATTCGAT CCAATCAGAC AATGGGCTGG GGAGAGAAGG CCATAGAGAT CCGATCTGTG 
GAAACTGGTC ACTTGGATGG TGTGTTCATG CACAAAAGGG CTCAAAGACT AAAATTCTTG 
TGTGAACGCA ATGACAAGGT GTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT 
TATTTCATGA CCTTAGGCAG GACTTCTCTT CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663719.2
CDS	379..4200
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	NM_145686.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145686.3

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA 
AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC 
TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT 
GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA 
CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
ATTTCTCCAT CTAGCGGAAC AACAGTGACA TCTGTGGTGG GATTTTCCTG TGATGGGATG 
AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT 
ACCAACACTA GGCCACAGAG TGACACCCCG GAGATTCGTA AATACAAGAA GAGGTTTAAC 
TCTGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC 
CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTTATCAA CCGAAGACGA 
TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG 
GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA 
GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT 
AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAGAG TTCTGTGGAA 
GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA 
TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG 
ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCAGGATC AGTCTATGAC 
ATTTATCTAC CAACACATAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC 
AATACAGATG GAATGGAGCT TCTGGTGTGC TATGAAGATG AGGGGGTTTA TGTAAACACA 
TATGGAAGGA TCACCAAGGA TGTAGTTCTA CAGTGGGGAG AGATGCCTAC ATCAGTAGCA 
TATATTCGAT CCAATCAGAC AATGGGCTGG GGAGAGAAGG CCATAGAGAT CCGATCTGTG 
GAAACTGGTC ACTTGGATGG TGTGTTCATG CACAAAAGGG CTCAAAGACT AAAATTCTTG 
TGTGAACGCA ATGACAAGGT GTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT 
TATTTCATGA CCTTAGGCAG GACTTCTCTT CTGAGCTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( NM_004834.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004825.3 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 1, mRNA.

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CloneID	OHu21057
Clone ID Related Accession (Same CDS sequence)	NM_004834.4 , NM_004834.5
Accession Version	NM_004834.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-29
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002278.1, AC007005.3, AF096300.1, AC005035.2 and BU625485.1. On Jun 14, 2011 this sequence version replaced NM_004834.3. Transcript Variant: This variant (1) lacks two alternate in-frame exons but includes a different in-frame exon in the central coding region, and uses an alternate in-frame splice site in both the central and 3' coding regions, compared to variant 2. The encoded isoform (1) is shorter than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF096300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGTA TTGAGCCCAG GCTTCTGTGG 
GAGAGAGTGG AGAAGCTGGT GCCCAGACCT GGCAGTGGCA GCTCCTCAGG GTCCAGCAAC 
TCAGGATCCC AGCCCGGGTC TCACCCTGGG TCTCAGAGTG GCTCCGGGGA ACGCTTCAGA 
GTGAGATCAT CATCCAAGTC TGAAGGCTCT CCATCTCAGC GCCTGGAAAA TGCAGTGAAA 
AAACCTGAAG ATAAAAAGGA AGTTTTCAGA CCCCTCAAGC CTGCTGGCGA AGTGGATCTG 
ACCGCACTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTAC GGCCACCTCA CAAAGTAACG 
GACTACTCCT CATCCAGTGA GGAGTCGGGG ACGACGGATG AGGAGGACGA CGATGTGGAG 
CAGGAAGGGG CTGACGAGTC CACCTCAGGA CCAGAGGACA CCAGAGCAGC GTCATCTCTG 
AATTTGAGCA ATGGTGAAAC GGAATCTGTG AAAACCATGA TTGTCCATGA TGATGTAGAA 
AGTGAGCCGG CCATGACCCC ATCCAAGGAG GGCACTCTAA TCGTCCGCCA GACTCAGTCC 
GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC 
AGATTACTAC AGATTTCTCC ATCTAGCGGA ACAACAGTGA CATCTGTGGT GGGATTTTCC 
TGTGATGGGA TGAGACCAGA AGCCATAAGG CAAGATCCTA CCCGGAAAGG CTCAGTGGTC 
AATGTGAATC CTACCAACAC TAGGCCACAG AGTGACACCC CGGAGATTCG TAAATACAAG 
AAGAGGTTTA ACTCTGAGAT TCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTGGGT 
ACAGAGAGTG GCCTGATGCT GCTGGACAGA AGTGGCCAAG GGAAGGTCTA TCCTCTTATC 
AACCGAAGAC GATTTCAACA AATGGACGTA CTTGAGGGCT TGAATGTCTT GGTGACAATA 
TCTGGCAAAA AGGATAAGTT ACGTGTCTAC TATTTGTCCT GGTTAAGAAA TAAAATACTT 
CACAATGATC CAGAAGTTGA GAAGAAGCAG GGATGGACAA CCGTAGGGGA TTTGGAAGGA 
TGTGTACATT ATAAAGTTGT AAAATATGAA AGAATCAAAT TTCTGGTGAT TGCTTTGAAG 
AGTTCTGTGG AAGTCTATGC GTGGGCACCA AAGCCATATC ACAAATTTAT GGCCTTTAAG 
TCATTTGGAG AATTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTTGA GGAAGGCCAG 
AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCAGGA 
TCAGTCTATG ACATTTATCT ACCAACACAT GTAAGAAAGA ACCCACACTC TATGATCCAG 
TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG 
GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA 
GTTCTACAGT GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG 
GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG 
TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC 
TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT 
TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004825.3
CDS	379..3876
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	NM_004834.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004834.4

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGTA TTGAGCCCAG GCTTCTGTGG 
GAGAGAGTGG AGAAGCTGGT GCCCAGACCT GGCAGTGGCA GCTCCTCAGG GTCCAGCAAC 
TCAGGATCCC AGCCCGGGTC TCACCCTGGG TCTCAGAGTG GCTCCGGGGA ACGCTTCAGA 
GTGAGATCAT CATCCAAGTC TGAAGGCTCT CCATCTCAGC GCCTGGAAAA TGCAGTGAAA 
AAACCTGAAG ATAAAAAGGA AGTTTTCAGA CCCCTCAAGC CTGCTGGCGA AGTGGATCTG 
ACCGCACTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTAC GGCCACCTCA CAAAGTAACG 
GACTACTCCT CATCCAGTGA GGAGTCGGGG ACGACGGATG AGGAGGACGA CGATGTGGAG 
CAGGAAGGGG CTGACGAGTC CACCTCAGGA CCAGAGGACA CCAGAGCAGC GTCATCTCTG 
AATTTGAGCA ATGGTGAAAC GGAATCTGTG AAAACCATGA TTGTCCATGA TGATGTAGAA 
AGTGAGCCGG CCATGACCCC ATCCAAGGAG GGCACTCTAA TCGTCCGCCA GACTCAGTCC 
GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC 
AGATTACTAC AGATTTCTCC ATCTAGCGGA ACAACAGTGA CATCTGTGGT GGGATTTTCC 
TGTGATGGGA TGAGACCAGA AGCCATAAGG CAAGATCCTA CCCGGAAAGG CTCAGTGGTC 
AATGTGAATC CTACCAACAC TAGGCCACAG AGTGACACCC CGGAGATTCG TAAATACAAG 
AAGAGGTTTA ACTCTGAGAT TCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTGGGT 
ACAGAGAGTG GCCTGATGCT GCTGGACAGA AGTGGCCAAG GGAAGGTCTA TCCTCTTATC 
AACCGAAGAC GATTTCAACA AATGGACGTA CTTGAGGGCT TGAATGTCTT GGTGACAATA 
TCTGGCAAAA AGGATAAGTT ACGTGTCTAC TATTTGTCCT GGTTAAGAAA TAAAATACTT 
CACAATGATC CAGAAGTTGA GAAGAAGCAG GGATGGACAA CCGTAGGGGA TTTGGAAGGA 
TGTGTACATT ATAAAGTTGT AAAATATGAA AGAATCAAAT TTCTGGTGAT TGCTTTGAAG 
AGTTCTGTGG AAGTCTATGC GTGGGCACCA AAGCCATATC ACAAATTTAT GGCCTTTAAG 
TCATTTGGAG AATTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTTGA GGAAGGCCAG 
AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCAGGA 
TCAGTCTATG ACATTTATCT ACCAACACAT GTAAGAAAGA ACCCACACTC TATGATCCAG 
TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG 
GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA 
GTTCTACAGT GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG 
GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG 
TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC 
TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT 
TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( NM_004834.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004825.3 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 1, mRNA.

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CloneID	OHu21057
Clone ID Related Accession (Same CDS sequence)	NM_004834.4 , NM_004834.5
Accession Version	NM_004834.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002278.1, AC007005.3, AF096300.1, AC005035.2 and BU625485.1. On Jun 2, 2019 this sequence version replaced NM_004834.4. Transcript Variant: This variant (1) lacks two alternate in-frame exons but includes a different in-frame exon in the central coding region, and uses an alternate in-frame splice site in both the central and 3' coding regions, compared to variant 2. The encoded isoform (1) is shorter than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF096300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGTA TTGAGCCCAG GCTTCTGTGG 
GAGAGAGTGG AGAAGCTGGT GCCCAGACCT GGCAGTGGCA GCTCCTCAGG GTCCAGCAAC 
TCAGGATCCC AGCCCGGGTC TCACCCTGGG TCTCAGAGTG GCTCCGGGGA ACGCTTCAGA 
GTGAGATCAT CATCCAAGTC TGAAGGCTCT CCATCTCAGC GCCTGGAAAA TGCAGTGAAA 
AAACCTGAAG ATAAAAAGGA AGTTTTCAGA CCCCTCAAGC CTGCTGGCGA AGTGGATCTG 
ACCGCACTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTAC GGCCACCTCA CAAAGTAACG 
GACTACTCCT CATCCAGTGA GGAGTCGGGG ACGACGGATG AGGAGGACGA CGATGTGGAG 
CAGGAAGGGG CTGACGAGTC CACCTCAGGA CCAGAGGACA CCAGAGCAGC GTCATCTCTG 
AATTTGAGCA ATGGTGAAAC GGAATCTGTG AAAACCATGA TTGTCCATGA TGATGTAGAA 
AGTGAGCCGG CCATGACCCC ATCCAAGGAG GGCACTCTAA TCGTCCGCCA GACTCAGTCC 
GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC 
AGATTACTAC AGATTTCTCC ATCTAGCGGA ACAACAGTGA CATCTGTGGT GGGATTTTCC 
TGTGATGGGA TGAGACCAGA AGCCATAAGG CAAGATCCTA CCCGGAAAGG CTCAGTGGTC 
AATGTGAATC CTACCAACAC TAGGCCACAG AGTGACACCC CGGAGATTCG TAAATACAAG 
AAGAGGTTTA ACTCTGAGAT TCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTGGGT 
ACAGAGAGTG GCCTGATGCT GCTGGACAGA AGTGGCCAAG GGAAGGTCTA TCCTCTTATC 
AACCGAAGAC GATTTCAACA AATGGACGTA CTTGAGGGCT TGAATGTCTT GGTGACAATA 
TCTGGCAAAA AGGATAAGTT ACGTGTCTAC TATTTGTCCT GGTTAAGAAA TAAAATACTT 
CACAATGATC CAGAAGTTGA GAAGAAGCAG GGATGGACAA CCGTAGGGGA TTTGGAAGGA 
TGTGTACATT ATAAAGTTGT AAAATATGAA AGAATCAAAT TTCTGGTGAT TGCTTTGAAG 
AGTTCTGTGG AAGTCTATGC GTGGGCACCA AAGCCATATC ACAAATTTAT GGCCTTTAAG 
TCATTTGGAG AATTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTTGA GGAAGGCCAG 
AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCAGGA 
TCAGTCTATG ACATTTATCT ACCAACACAT GTAAGAAAGA ACCCACACTC TATGATCCAG 
TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG 
GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA 
GTTCTACAGT GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG 
GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG 
TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC 
TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT 
TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004825.3
CDS	375..3872
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	NM_004834.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004834.5

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGTA TTGAGCCCAG GCTTCTGTGG 
GAGAGAGTGG AGAAGCTGGT GCCCAGACCT GGCAGTGGCA GCTCCTCAGG GTCCAGCAAC 
TCAGGATCCC AGCCCGGGTC TCACCCTGGG TCTCAGAGTG GCTCCGGGGA ACGCTTCAGA 
GTGAGATCAT CATCCAAGTC TGAAGGCTCT CCATCTCAGC GCCTGGAAAA TGCAGTGAAA 
AAACCTGAAG ATAAAAAGGA AGTTTTCAGA CCCCTCAAGC CTGCTGGCGA AGTGGATCTG 
ACCGCACTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTAC GGCCACCTCA CAAAGTAACG 
GACTACTCCT CATCCAGTGA GGAGTCGGGG ACGACGGATG AGGAGGACGA CGATGTGGAG 
CAGGAAGGGG CTGACGAGTC CACCTCAGGA CCAGAGGACA CCAGAGCAGC GTCATCTCTG 
AATTTGAGCA ATGGTGAAAC GGAATCTGTG AAAACCATGA TTGTCCATGA TGATGTAGAA 
AGTGAGCCGG CCATGACCCC ATCCAAGGAG GGCACTCTAA TCGTCCGCCA GACTCAGTCC 
GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC 
AGATTACTAC AGATTTCTCC ATCTAGCGGA ACAACAGTGA CATCTGTGGT GGGATTTTCC 
TGTGATGGGA TGAGACCAGA AGCCATAAGG CAAGATCCTA CCCGGAAAGG CTCAGTGGTC 
AATGTGAATC CTACCAACAC TAGGCCACAG AGTGACACCC CGGAGATTCG TAAATACAAG 
AAGAGGTTTA ACTCTGAGAT TCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTGGGT 
ACAGAGAGTG GCCTGATGCT GCTGGACAGA AGTGGCCAAG GGAAGGTCTA TCCTCTTATC 
AACCGAAGAC GATTTCAACA AATGGACGTA CTTGAGGGCT TGAATGTCTT GGTGACAATA 
TCTGGCAAAA AGGATAAGTT ACGTGTCTAC TATTTGTCCT GGTTAAGAAA TAAAATACTT 
CACAATGATC CAGAAGTTGA GAAGAAGCAG GGATGGACAA CCGTAGGGGA TTTGGAAGGA 
TGTGTACATT ATAAAGTTGT AAAATATGAA AGAATCAAAT TTCTGGTGAT TGCTTTGAAG 
AGTTCTGTGG AAGTCTATGC GTGGGCACCA AAGCCATATC ACAAATTTAT GGCCTTTAAG 
TCATTTGGAG AATTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTTGA GGAAGGCCAG 
AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCAGGA 
TCAGTCTATG ACATTTATCT ACCAACACAT GTAAGAAAGA ACCCACACTC TATGATCCAG 
TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG 
GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA 
GTTCTACAGT GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG 
GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG 
TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC 
TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT 
TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( NM_001242560.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229489.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 5, mRNA.

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CloneID	OHu21579
Clone ID Related Accession (Same CDS sequence)	NM_001242560.2 , NM_001242560.1
Accession Version	NM_001242560.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002278.1, AC007005.3, AK296396.1, AC005035.2 and BU625485.1. On Jun 2, 2019 this sequence version replaced NM_001242560.1. Transcript Variant: This variant (5) lacks two alternate in-frame exons but includes two different alternate in-frame exons in the central coding region, compared to variant 2. The encoded isoform (5) is shorter than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296396.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG 
TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC 
CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA 
AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA 
TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT 
AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA 
GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA 
TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA 
TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT 
GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA 
CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC 
TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA 
GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG 
AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG 
CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT 
AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA 
CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT 
GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG 
AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG 
TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG 
AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA 
AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC 
AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT 
GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA 
CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT 
GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT 
GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG 
AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC 
TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA AACCCCATGC AATCATCATC 
CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA 
AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA 
GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA 
TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA 
TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT 
CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229489.1
CDS	375..4082
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	NM_001242560.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242560.2

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG 
TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC 
CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA 
AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA 
TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT 
AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA 
GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA 
TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA 
TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT 
GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA 
CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC 
TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA 
GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG 
AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG 
CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT 
AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA 
CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT 
GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG 
AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG 
TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG 
AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA 
AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC 
AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT 
GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA 
CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT 
GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT 
GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG 
AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC 
TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA AACCCCATGC AATCATCATC 
CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA 
AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA 
GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA 
TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA 
TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT 
CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( NM_001242560.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229489.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 5, mRNA.

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CloneID	OHu21579
Clone ID Related Accession (Same CDS sequence)	NM_001242560.2 , NM_001242560.1
Accession Version	NM_001242560.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-29
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002278.1, AC007005.3, AK296396.1, AC005035.2 and BU625485.1. Transcript Variant: This variant (5) lacks two alternate in-frame exons but includes two different alternate in-frame exons in the central coding region, compared to variant 2. The encoded isoform (5) is shorter than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296396.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG 
TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC 
CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA 
AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA 
TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT 
AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA 
GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA 
TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA 
TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT 
GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA 
CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC 
TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA 
GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG 
AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG 
CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT 
AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA 
CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT 
GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG 
AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG 
TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG 
AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA 
AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC 
AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT 
GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA 
CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT 
GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT 
GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG 
AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC 
TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA AACCCCATGC AATCATCATC 
CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA 
AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA 
GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA 
TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA 
TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT 
CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229489.1
CDS	379..4086
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	NM_001242560.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242560.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG 
TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC 
CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA 
AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA 
TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT 
AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA 
GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA 
TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA 
TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT 
GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA 
CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC 
TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA 
GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG 
AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG 
CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT 
AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA 
CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT 
GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG 
AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG 
TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG 
AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA 
AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC 
AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT 
GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA 
CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT 
GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT 
GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG 
AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC 
TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA AACCCCATGC AATCATCATC 
CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA 
AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA 
GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA 
TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA 
TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT 
CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( NM_001242559.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229488.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 4, mRNA.

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CloneID	OHu22392
Clone ID Related Accession (Same CDS sequence)	NM_001242559.1 , NM_001242559.2
Accession Version	NM_001242559.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB086475.1, AY212247.1, AC005035.2 and BU625485.1. Transcript Variant: This variant (4) uses two alternate in-frame splice sites and lacks an alternate in-frame exon in the central coding region, compared to variant 2. The encoded isoform (4) is shorter than isoform 2. The 5' UTR of this variant is incomplete due to a lack of 5'-complete transcripts representing this variant, and the presence of splicing ambiguity in the 5' UTR. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY212247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG 
CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC 
TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC 
GGGGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG 
GAAAATGCAG TGAAAAAACC TGAAGATAAA AAGGAAGTTT TCAGACCCCT CAAGCCTGCT 
GATCTGACCG CACTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTACGGCC ACCTCACAAA 
GTAACGGACT ACTCCTCATC CAGTGAGGAG TCGGGGACGA CGGATGAGGA GGACGACGAT 
GTGGAGCAGG AAGGGGCTGA CGAGTCCACC TCAGGACCAG AGGACACCAG AGCAGCGTCA 
TCTCTGAATT TGAGCAATGG TGAAACGGAA TCTGTGAAAA CCATGATTGT CCATGATGAT 
GTAGAAAGTG AGCCGGCCAT GACCCCATCC AAGGAGGGCA CTCTAATCGT CCGCCAGACT 
CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA 
TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA 
GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA 
TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA 
GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT 
CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG 
ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG 
GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT 
TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC 
TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA 
GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT 
TCAGGATCAG TCTATGACAT TTATCTACCA ACACATATCC AGTGTAGCAT CAAACCCCAT 
GCAATCATCA TCCTCCCCAA TACAGATGGA ATGGAGCTTC TGGTGTGCTA TGAAGATGAG 
GGGGTTTATG TAAACACATA TGGAAGGATC ACCAAGGATG TAGTTCTACA GTGGGGAGAG 
ATGCCTACAT CAGTAGCATA TATTCGATCC AATCAGACAA TGGGCTGGGG AGAGAAGGCC 
ATAGAGATCC GATCTGTGGA AACTGGTCAC TTGGATGGTG TGTTCATGCA CAAAAGGGCT 
CAAAGACTAA AATTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC TGTTCGGTCT 
GGTGGCAGCA GTCAGGTTTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229488.1
CDS	6..3725
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	NM_001242559.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242559.1

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG 
CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC 
TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC 
GGGGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG 
GAAAATGCAG TGAAAAAACC TGAAGATAAA AAGGAAGTTT TCAGACCCCT CAAGCCTGCT 
GATCTGACCG CACTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTACGGCC ACCTCACAAA 
GTAACGGACT ACTCCTCATC CAGTGAGGAG TCGGGGACGA CGGATGAGGA GGACGACGAT 
GTGGAGCAGG AAGGGGCTGA CGAGTCCACC TCAGGACCAG AGGACACCAG AGCAGCGTCA 
TCTCTGAATT TGAGCAATGG TGAAACGGAA TCTGTGAAAA CCATGATTGT CCATGATGAT 
GTAGAAAGTG AGCCGGCCAT GACCCCATCC AAGGAGGGCA CTCTAATCGT CCGCCAGACT 
CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA 
TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA 
GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA 
TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA 
GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT 
CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG 
ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG 
GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT 
TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC 
TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA 
GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT 
TCAGGATCAG TCTATGACAT TTATCTACCA ACACATATCC AGTGTAGCAT CAAACCCCAT 
GCAATCATCA TCCTCCCCAA TACAGATGGA ATGGAGCTTC TGGTGTGCTA TGAAGATGAG 
GGGGTTTATG TAAACACATA TGGAAGGATC ACCAAGGATG TAGTTCTACA GTGGGGAGAG 
ATGCCTACAT CAGTAGCATA TATTCGATCC AATCAGACAA TGGGCTGGGG AGAGAAGGCC 
ATAGAGATCC GATCTGTGGA AACTGGTCAC TTGGATGGTG TGTTCATGCA CAAAAGGGCT 
CAAAGACTAA AATTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC TGTTCGGTCT 
GGTGGCAGCA GTCAGGTTTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( NM_001242559.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001229488.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 4, mRNA.

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CloneID	OHu22392
Clone ID Related Accession (Same CDS sequence)	NM_001242559.1 , NM_001242559.2
Accession Version	NM_001242559.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007005.3, DB086475.1, AY212247.1, AC005035.2 and BU625485.1. On Jun 2, 2019 this sequence version replaced NM_001242559.1. Transcript Variant: This variant (4) uses two alternate in-frame splice sites and lacks an alternate in-frame exon in the central coding region, compared to variant 2. The encoded isoform (4) is shorter than isoform 2. The 5' UTR of this variant is incomplete due to a lack of 5'-complete transcripts representing this variant, and the presence of splicing ambiguity in the 5' UTR. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY212247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG 
CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC 
TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC 
GGGGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG 
GAAAATGCAG TGAAAAAACC TGAAGATAAA AAGGAAGTTT TCAGACCCCT CAAGCCTGCT 
GATCTGACCG CACTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTACGGCC ACCTCACAAA 
GTAACGGACT ACTCCTCATC CAGTGAGGAG TCGGGGACGA CGGATGAGGA GGACGACGAT 
GTGGAGCAGG AAGGGGCTGA CGAGTCCACC TCAGGACCAG AGGACACCAG AGCAGCGTCA 
TCTCTGAATT TGAGCAATGG TGAAACGGAA TCTGTGAAAA CCATGATTGT CCATGATGAT 
GTAGAAAGTG AGCCGGCCAT GACCCCATCC AAGGAGGGCA CTCTAATCGT CCGCCAGACT 
CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA 
TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA 
GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA 
TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA 
GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT 
CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG 
ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG 
GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT 
TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC 
TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA 
GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT 
TCAGGATCAG TCTATGACAT TTATCTACCA ACACATATCC AGTGTAGCAT CAAACCCCAT 
GCAATCATCA TCCTCCCCAA TACAGATGGA ATGGAGCTTC TGGTGTGCTA TGAAGATGAG 
GGGGTTTATG TAAACACATA TGGAAGGATC ACCAAGGATG TAGTTCTACA GTGGGGAGAG 
ATGCCTACAT CAGTAGCATA TATTCGATCC AATCAGACAA TGGGCTGGGG AGAGAAGGCC 
ATAGAGATCC GATCTGTGGA AACTGGTCAC TTGGATGGTG TGTTCATGCA CAAAAGGGCT 
CAAAGACTAA AATTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC TGTTCGGTCT 
GGTGGCAGCA GTCAGGTTTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001229488.1
CDS	375..4094
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	NM_001242559.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001242559.2

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG 
CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC 
TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC 
GGGGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG 
GAAAATGCAG TGAAAAAACC TGAAGATAAA AAGGAAGTTT TCAGACCCCT CAAGCCTGCT 
GATCTGACCG CACTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTACGGCC ACCTCACAAA 
GTAACGGACT ACTCCTCATC CAGTGAGGAG TCGGGGACGA CGGATGAGGA GGACGACGAT 
GTGGAGCAGG AAGGGGCTGA CGAGTCCACC TCAGGACCAG AGGACACCAG AGCAGCGTCA 
TCTCTGAATT TGAGCAATGG TGAAACGGAA TCTGTGAAAA CCATGATTGT CCATGATGAT 
GTAGAAAGTG AGCCGGCCAT GACCCCATCC AAGGAGGGCA CTCTAATCGT CCGCCAGACT 
CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA 
TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA 
GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA 
TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA 
GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT 
CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG 
ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG 
GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT 
TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC 
TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA 
GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT 
TCAGGATCAG TCTATGACAT TTATCTACCA ACACATATCC AGTGTAGCAT CAAACCCCAT 
GCAATCATCA TCCTCCCCAA TACAGATGGA ATGGAGCTTC TGGTGTGCTA TGAAGATGAG 
GGGGTTTATG TAAACACATA TGGAAGGATC ACCAAGGATG TAGTTCTACA GTGGGGAGAG 
ATGCCTACAT CAGTAGCATA TATTCGATCC AATCAGACAA TGGGCTGGGG AGAGAAGGCC 
ATAGAGATCC GATCTGTGGA AACTGGTCAC TTGGATGGTG TGTTCATGCA CAAAAGGGCT 
CAAAGACTAA AATTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC TGTTCGGTCT 
GGTGGCAGCA GTCAGGTTTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_005264057.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264114.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu36834
Clone ID Related Accession (Same CDS sequence)	XM_005264057.3
Accession Version	XM_005264057.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264057.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT 
GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC 
TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC 
TCCGGGGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC 
CTGGAAAATG CAGTGAAAAA ACCTGAAGAT AAAAAGGAAG TTTTCAGACC CCTCAAGCCT 
GCTGGCGAAG TGGATCTGAC CGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTACGG 
CCACCTCACA AAGTAACGGA CTACTCCTCA TCCAGTGAGG AGTCGGGGAC GACGGATGAG 
GAGGACGACG ATGTGGAGCA GGAAGGGGCT GACGAGTCCA CCTCAGGACC AGAGGACACC 
AGAGCAGCGT CATCTCTGAA TTTGAGCAAT GGTGAAACGG AATCTGTGAA AACCATGATT 
GTCCATGATG ATGTAGAAAG TGAGCCGGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC 
GTCCGCCAGA GTACAGTTGA CCAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCC 
GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC 
ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG 
GACAAGCTCA CTGCTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT 
TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGCGGA 
ACAACAGTGA CATCTGTGGT GGGATTTTCC TGTGATGGGA TGAGACCAGA AGCCATAAGG 
CAAGATCCTA CCCGGAAAGG CTCAGTGGTC AATGTGAATC CTACCAACAC TAGGCCACAG 
AGTGACACCC CGGAGATTCG TAAATACAAG AAGAGGTTTA ACTCTGAGAT TCTGTGTGCT 
GCCTTATGGG GAGTGAATTT GCTAGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA 
AGTGGCCAAG GGAAGGTCTA TCCTCTTATC AACCGAAGAC GATTTCAACA AATGGACGTA 
CTTGAGGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC 
TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAATGATC CAGAAGTTGA GAAGAAGCAG 
GGATGGACAA CCGTAGGGGA TTTGGAAGGA TGTGTACATT ATAAAGTTGT AAAATATGAA 
AGAATCAAAT TTCTGGTGAT TGCTTTGAAG AGTTCTGTGG AAGTCTATGC GTGGGCACCA 
AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AATTGGTACA TAAGCCATTA 
CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT 
GGATTCCATG CTGTTGATGT GGATTCAGGA TCAGTCTATG ACATTTATCT ACCAACACAT 
GTAAGAAAGA ACCCACACTC TATGATCCAG TGTAGCATCA AACCCCATGC AATCATCATC 
CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA 
AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA 
GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA 
TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA 
TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT 
CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264114.1
CDS	75..4022
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_005264057.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264057.3

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT 
GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC 
TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC 
TCCGGGGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC 
CTGGAAAATG CAGTGAAAAA ACCTGAAGAT AAAAAGGAAG TTTTCAGACC CCTCAAGCCT 
GCTGGCGAAG TGGATCTGAC CGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTACGG 
CCACCTCACA AAGTAACGGA CTACTCCTCA TCCAGTGAGG AGTCGGGGAC GACGGATGAG 
GAGGACGACG ATGTGGAGCA GGAAGGGGCT GACGAGTCCA CCTCAGGACC AGAGGACACC 
AGAGCAGCGT CATCTCTGAA TTTGAGCAAT GGTGAAACGG AATCTGTGAA AACCATGATT 
GTCCATGATG ATGTAGAAAG TGAGCCGGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC 
GTCCGCCAGA GTACAGTTGA CCAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCC 
GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC 
ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG 
GACAAGCTCA CTGCTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT 
TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGCGGA 
ACAACAGTGA CATCTGTGGT GGGATTTTCC TGTGATGGGA TGAGACCAGA AGCCATAAGG 
CAAGATCCTA CCCGGAAAGG CTCAGTGGTC AATGTGAATC CTACCAACAC TAGGCCACAG 
AGTGACACCC CGGAGATTCG TAAATACAAG AAGAGGTTTA ACTCTGAGAT TCTGTGTGCT 
GCCTTATGGG GAGTGAATTT GCTAGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA 
AGTGGCCAAG GGAAGGTCTA TCCTCTTATC AACCGAAGAC GATTTCAACA AATGGACGTA 
CTTGAGGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC 
TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAATGATC CAGAAGTTGA GAAGAAGCAG 
GGATGGACAA CCGTAGGGGA TTTGGAAGGA TGTGTACATT ATAAAGTTGT AAAATATGAA 
AGAATCAAAT TTCTGGTGAT TGCTTTGAAG AGTTCTGTGG AAGTCTATGC GTGGGCACCA 
AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AATTGGTACA TAAGCCATTA 
CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT 
GGATTCCATG CTGTTGATGT GGATTCAGGA TCAGTCTATG ACATTTATCT ACCAACACAT 
GTAAGAAAGA ACCCACACTC TATGATCCAG TGTAGCATCA AACCCCATGC AATCATCATC 
CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA 
AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA 
GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA 
TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA 
TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT 
CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_005264058.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264115.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.

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CloneID	OHu36835
Clone ID Related Accession (Same CDS sequence)	XM_005264058.3
Accession Version	XM_005264058.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264058.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT 
GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC 
TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC 
TCCGGGGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC 
CTGGAAAATG CAGTGAAAAA ACCTGAAGAT AAAAAGGAAG TTTTCAGACC CCTCAAGCCT 
GCTGATCTGA CCGCACTGGC CAAAGAGCTT CGAGCAGTGG AAGATGTACG GCCACCTCAC 
AAAGTAACGG ACTACTCCTC ATCCAGTGAG GAGTCGGGGA CGACGGATGA GGAGGACGAC 
GATGTGGAGC AGGAAGGGGC TGACGAGTCC ACCTCAGGAC CAGAGGACAC CAGAGCAGCG 
TCATCTCTGA ATTTGAGCAA TGGTGAAACG GAATCTGTGA AAACCATGAT TGTCCATGAT 
GATGTAGAAA GTGAGCCGGC CATGACCCCA TCCAAGGAGG GCACTCTAAT CGTCCGCCAG 
AGTACAGTTG ACCAAAAGCG TGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT 
CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC 
TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC 
ACTGCTAATG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC 
TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGCGG AACAACAGTG 
ACATCTGTGG TGGGATTTTC CTGTGATGGG ATGAGACCAG AAGCCATAAG GCAAGATCCT 
ACCCGGAAAG GCTCAGTGGT CAATGTGAAT CCTACCAACA CTAGGCCACA GAGTGACACC 
CCGGAGATTC GTAAATACAA GAAGAGGTTT AACTCTGAGA TTCTGTGTGC TGCCTTATGG 
GGAGTGAATT TGCTAGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA 
GGGAAGGTCT ATCCTCTTAT CAACCGAAGA CGATTTCAAC AAATGGACGT ACTTGAGGGC 
TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC 
TGGTTAAGAA ATAAAATACT TCACAATGAT CCAGAAGTTG AGAAGAAGCA GGGATGGACA 
ACCGTAGGGG ATTTGGAAGG ATGTGTACAT TATAAAGTTG TAAAATATGA AAGAATCAAA 
TTTCTGGTGA TTGCTTTGAA GAGTTCTGTG GAAGTCTATG CGTGGGCACC AAAGCCATAT 
CACAAATTTA TGGCCTTTAA GTCATTTGGA GAATTGGTAC ATAAGCCATT ACTGGTGGAT 
CTCACTGTTG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT 
GCTGTTGATG TGGATTCAGG ATCAGTCTAT GACATTTATC TACCAACACA TGTAAGAAAG 
AACCCACACT CTATGATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT 
ACAGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT 
GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACATC AGTAGCATAT 
ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA 
ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT 
GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT 
TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264115.1
CDS	75..4013
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_005264058.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264058.3

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA 
CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT 
GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC 
TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC 
TCCGGGGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC 
CTGGAAAATG CAGTGAAAAA ACCTGAAGAT AAAAAGGAAG TTTTCAGACC CCTCAAGCCT 
GCTGATCTGA CCGCACTGGC CAAAGAGCTT CGAGCAGTGG AAGATGTACG GCCACCTCAC 
AAAGTAACGG ACTACTCCTC ATCCAGTGAG GAGTCGGGGA CGACGGATGA GGAGGACGAC 
GATGTGGAGC AGGAAGGGGC TGACGAGTCC ACCTCAGGAC CAGAGGACAC CAGAGCAGCG 
TCATCTCTGA ATTTGAGCAA TGGTGAAACG GAATCTGTGA AAACCATGAT TGTCCATGAT 
GATGTAGAAA GTGAGCCGGC CATGACCCCA TCCAAGGAGG GCACTCTAAT CGTCCGCCAG 
AGTACAGTTG ACCAAAAGCG TGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT 
CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC 
TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC 
ACTGCTAATG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC 
TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGCGG AACAACAGTG 
ACATCTGTGG TGGGATTTTC CTGTGATGGG ATGAGACCAG AAGCCATAAG GCAAGATCCT 
ACCCGGAAAG GCTCAGTGGT CAATGTGAAT CCTACCAACA CTAGGCCACA GAGTGACACC 
CCGGAGATTC GTAAATACAA GAAGAGGTTT AACTCTGAGA TTCTGTGTGC TGCCTTATGG 
GGAGTGAATT TGCTAGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA 
GGGAAGGTCT ATCCTCTTAT CAACCGAAGA CGATTTCAAC AAATGGACGT ACTTGAGGGC 
TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC 
TGGTTAAGAA ATAAAATACT TCACAATGAT CCAGAAGTTG AGAAGAAGCA GGGATGGACA 
ACCGTAGGGG ATTTGGAAGG ATGTGTACAT TATAAAGTTG TAAAATATGA AAGAATCAAA 
TTTCTGGTGA TTGCTTTGAA GAGTTCTGTG GAAGTCTATG CGTGGGCACC AAAGCCATAT 
CACAAATTTA TGGCCTTTAA GTCATTTGGA GAATTGGTAC ATAAGCCATT ACTGGTGGAT 
CTCACTGTTG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT 
GCTGTTGATG TGGATTCAGG ATCAGTCTAT GACATTTATC TACCAACACA TGTAAGAAAG 
AACCCACACT CTATGATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT 
ACAGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT 
GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACATC AGTAGCATAT 
ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA 
ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT 
GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT 
TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_006712865.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712928.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu36836
Clone ID Related Accession (Same CDS sequence)	XM_006712865.2
Accession Version	XM_006712865.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006712865.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG 
TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC 
CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA 
AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA 
TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT 
AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA 
GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA 
TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA 
TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT 
GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA 
CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC 
TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA 
GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG 
AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG 
CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGAGTAC AGTTGACCAA 
AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCCGGTC GCATTCACCT CTTGCCAGAT 
CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCCTCCCC ATCCTCCAGC 
CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT 
CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA 
TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA 
GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA 
TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA 
GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT 
CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG 
ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG 
GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT 
TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC 
TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA 
GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT 
TCAGGATCAG TCTATGACAT TTATCTACCA ACACATGTAA GAAAGAACCC ACACTCTATG 
ATCCAGTGTA GCATCAAACC CCATGCAATC ATCATCCTCC CCAATACAGA TGGAATGGAG 
CTTCTGGTGT GCTATGAAGA TGAGGGGGTT TATGTAAACA CATATGGAAG GATCACCAAG 
GATGTAGTTC TACAGTGGGG AGAGATGCCT ACATCAGTAG CATATATTCG ATCCAATCAG 
ACAATGGGCT GGGGAGAGAA GGCCATAGAG ATCCGATCTG TGGAAACTGG TCACTTGGAT 
GGTGTGTTCA TGCACAAAAG GGCTCAAAGA CTAAAATTCT TGTGTGAACG CAATGACAAG 
GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC AGCAGTCAGG TTTATTTCAT GACCTTAGGC 
AGGACTTCTC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712928.1
CDS	75..3998
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_006712865.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712865.2

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG 
TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT 
GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC 
CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA 
AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA 
TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT 
AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA 
GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA 
TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA 
TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT 
GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA 
CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC 
TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA 
GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG 
AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG 
CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGAGTAC AGTTGACCAA 
AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCCGGTC GCATTCACCT CTTGCCAGAT 
CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCCTCCCC ATCCTCCAGC 
CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT 
CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA 
TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA 
GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA 
TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA 
GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT 
CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG 
ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG 
GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT 
TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC 
TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA 
GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT 
TCAGGATCAG TCTATGACAT TTATCTACCA ACACATGTAA GAAAGAACCC ACACTCTATG 
ATCCAGTGTA GCATCAAACC CCATGCAATC ATCATCCTCC CCAATACAGA TGGAATGGAG 
CTTCTGGTGT GCTATGAAGA TGAGGGGGTT TATGTAAACA CATATGGAAG GATCACCAAG 
GATGTAGTTC TACAGTGGGG AGAGATGCCT ACATCAGTAG CATATATTCG ATCCAATCAG 
ACAATGGGCT GGGGAGAGAA GGCCATAGAG ATCCGATCTG TGGAAACTGG TCACTTGGAT 
GGTGTGTTCA TGCACAAAAG GGCTCAAAGA CTAAAATTCT TGTGTGAACG CAATGACAAG 
GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC AGCAGTCAGG TTTATTTCAT GACCTTAGGC 
AGGACTTCTC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_005264059.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264116.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu36838
Clone ID Related Accession (Same CDS sequence)	XM_005264059.3
Accession Version	XM_005264059.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264059.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA 
AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA 
GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC 
AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC 
GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT 
GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG 
ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGAGTA CAGTTGACCA AAAGCGTGCC 
AGCCATCATG AGAGCAATGG CTTTGCCGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG 
CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCCTCCC CATCCTCCAG CCAGCCGACA 
CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTG CTAATGAGAC TCAGTCCGCT 
AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
TTACTACAGA TTTCTCCATC TAGCGGAACA ACAGTGACAT CTGTGGTGGG ATTTTCCTGT 
GATGGGATGA GACCAGAAGC CATAAGGCAA GATCCTACCC GGAAAGGCTC AGTGGTCAAT 
GTGAATCCTA CCAACACTAG GCCACAGAGT GACACCCCGG AGATTCGTAA ATACAAGAAG 
AGGTTTAACT CTGAGATTCT GTGTGCTGCC TTATGGGGAG TGAATTTGCT AGTGGGTACA 
GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTCTATCC TCTTATCAAC 
CGAAGACGAT TTCAACAAAT GGACGTACTT GAGGGCTTGA ATGTCTTGGT GACAATATCT 
GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACAACCG TAGGGGATTT GGAAGGATGT 
GTACATTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC TGGTGATTGC TTTGAAGAGT 
TCTGTGGAAG TCTATGCGTG GGCACCAAAG CCATATCACA AATTTATGGC CTTTAAGTCA 
TTTGGAGAAT TGGTACATAA GCCATTACTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA TTCAGGATCA 
GTCTATGACA TTTATCTACC AACACATGTA AGAAAGAACC CACACTCTAT GATCCAGTGT 
AGCATCAAAC CCCATGCAAT CATCATCCTC CCCAATACAG ATGGAATGGA GCTTCTGGTG 
TGCTATGAAG ATGAGGGGGT TTATGTAAAC ACATATGGAA GGATCACCAA GGATGTAGTT 
CTACAGTGGG GAGAGATGCC TACATCAGTA GCATATATTC GATCCAATCA GACAATGGGC 
TGGGGAGAGA AGGCCATAGA GATCCGATCT GTGGAAACTG GTCACTTGGA TGGTGTGTTC 
ATGCACAAAA GGGCTCAAAG ACTAAAATTC TTGTGTGAAC GCAATGACAA GGTGTTCTTT 
GCCTCTGTTC GGTCTGGTGG CAGCAGTCAG GTTTATTTCA TGACCTTAGG CAGGACTTCT 
CTTCTGAGCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264116.1
CDS	75..3929
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_005264059.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264059.3

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA 
AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA 
GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC 
AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC 
GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT 
GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG 
ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGAGTA CAGTTGACCA AAAGCGTGCC 
AGCCATCATG AGAGCAATGG CTTTGCCGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG 
CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCCTCCC CATCCTCCAG CCAGCCGACA 
CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTG CTAATGAGAC TCAGTCCGCT 
AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
TTACTACAGA TTTCTCCATC TAGCGGAACA ACAGTGACAT CTGTGGTGGG ATTTTCCTGT 
GATGGGATGA GACCAGAAGC CATAAGGCAA GATCCTACCC GGAAAGGCTC AGTGGTCAAT 
GTGAATCCTA CCAACACTAG GCCACAGAGT GACACCCCGG AGATTCGTAA ATACAAGAAG 
AGGTTTAACT CTGAGATTCT GTGTGCTGCC TTATGGGGAG TGAATTTGCT AGTGGGTACA 
GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTCTATCC TCTTATCAAC 
CGAAGACGAT TTCAACAAAT GGACGTACTT GAGGGCTTGA ATGTCTTGGT GACAATATCT 
GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACAACCG TAGGGGATTT GGAAGGATGT 
GTACATTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC TGGTGATTGC TTTGAAGAGT 
TCTGTGGAAG TCTATGCGTG GGCACCAAAG CCATATCACA AATTTATGGC CTTTAAGTCA 
TTTGGAGAAT TGGTACATAA GCCATTACTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA TTCAGGATCA 
GTCTATGACA TTTATCTACC AACACATGTA AGAAAGAACC CACACTCTAT GATCCAGTGT 
AGCATCAAAC CCCATGCAAT CATCATCCTC CCCAATACAG ATGGAATGGA GCTTCTGGTG 
TGCTATGAAG ATGAGGGGGT TTATGTAAAC ACATATGGAA GGATCACCAA GGATGTAGTT 
CTACAGTGGG GAGAGATGCC TACATCAGTA GCATATATTC GATCCAATCA GACAATGGGC 
TGGGGAGAGA AGGCCATAGA GATCCGATCT GTGGAAACTG GTCACTTGGA TGGTGTGTTC 
ATGCACAAAA GGGCTCAAAG ACTAAAATTC TTGTGTGAAC GCAATGACAA GGTGTTCTTT 
GCCTCTGTTC GGTCTGGTGG CAGCAGTCAG GTTTATTTCA TGACCTTAGG CAGGACTTCT 
CTTCTGAGCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_005264060.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264117.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA.

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CloneID	OHu36839
Clone ID Related Accession (Same CDS sequence)	XM_005264060.3
Accession Version	XM_005264060.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264060.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG TATTGAGCCC AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG 
GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA GGGTCCAGCA ACTCAGGATC CCAGCCCGGG 
TCTCACCCTG GGTCTCAGAG TGGCTCCGGG GAACGCTTCA GAGTGAGATC ATCATCCAAG 
TCTGAAGGCT CTCCATCTCA GCGCCTGGAA AATGCAGTGA AAAAACCTGA AGATAAAAAG 
GAAGTTTTCA GACCCCTCAA GCCTGCTGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA 
GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG 
GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA 
GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT 
GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG 
GAGGGCACTC TAATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG 
AGCAATGGCT TTGCCGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC 
TCCTCCACTT CCTCCACCTC CTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC 
CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC 
CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA 
CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC 
AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT 
GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG 
ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT 
CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT 
AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA 
GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA 
GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC 
TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG 
GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC 
TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT 
TATCTACCAA CACATGTAAG AAAGAACCCA CACTCTATGA TCCAGTGTAG CATCAAACCC 
CATGCAATCA TCATCCTCCC CAATACAGAT GGAATGGAGC TTCTGGTGTG CTATGAAGAT 
GAGGGGGTTT ATGTAAACAC ATATGGAAGG ATCACCAAGG ATGTAGTTCT ACAGTGGGGA 
GAGATGCCTA CATCAGTAGC ATATATTCGA TCCAATCAGA CAATGGGCTG GGGAGAGAAG 
GCCATAGAGA TCCGATCTGT GGAAACTGGT CACTTGGATG GTGTGTTCAT GCACAAAAGG 
GCTCAAAGAC TAAAATTCTT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCTGTTCGG 
TCTGGTGGCA GCAGTCAGGT TTATTTCATG ACCTTAGGCA GGACTTCTCT TCTGAGCTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264117.1
CDS	75..3917
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_005264060.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264060.3

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG TATTGAGCCC AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG 
GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA GGGTCCAGCA ACTCAGGATC CCAGCCCGGG 
TCTCACCCTG GGTCTCAGAG TGGCTCCGGG GAACGCTTCA GAGTGAGATC ATCATCCAAG 
TCTGAAGGCT CTCCATCTCA GCGCCTGGAA AATGCAGTGA AAAAACCTGA AGATAAAAAG 
GAAGTTTTCA GACCCCTCAA GCCTGCTGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA 
GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG 
GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA 
GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT 
GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG 
GAGGGCACTC TAATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG 
AGCAATGGCT TTGCCGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC 
TCCTCCACTT CCTCCACCTC CTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC 
CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC 
CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA 
CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC 
AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT 
GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG 
ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT 
CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT 
AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA 
GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA 
GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC 
TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG 
GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC 
TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT 
TATCTACCAA CACATGTAAG AAAGAACCCA CACTCTATGA TCCAGTGTAG CATCAAACCC 
CATGCAATCA TCATCCTCCC CAATACAGAT GGAATGGAGC TTCTGGTGTG CTATGAAGAT 
GAGGGGGTTT ATGTAAACAC ATATGGAAGG ATCACCAAGG ATGTAGTTCT ACAGTGGGGA 
GAGATGCCTA CATCAGTAGC ATATATTCGA TCCAATCAGA CAATGGGCTG GGGAGAGAAG 
GCCATAGAGA TCCGATCTGT GGAAACTGGT CACTTGGATG GTGTGTTCAT GCACAAAAGG 
GCTCAAAGAC TAAAATTCTT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCTGTTCGG 
TCTGGTGGCA GCAGTCAGGT TTATTTCATG ACCTTAGGCA GGACTTCTCT TCTGAGCTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_005264062.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264119.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OHu36841
Clone ID Related Accession (Same CDS sequence)	XM_005264062.3
Accession Version	XM_005264062.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264062.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC 
AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC 
CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA 
GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT 
TTCAGACCCC TCAAGCCTGC TGGCGAAGTG GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC 
TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT 
GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA 
CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
ATTTCTCCAT CTAGCGGAAC AACAGTGACA TCTGTGGTGG GATTTTCCTG TGATGGGATG 
AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT 
ACCAACACTA GGCCACAGAG TGACACCCCG GAGATTCGTA AATACAAGAA GAGGTTTAAC 
TCTGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC 
CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTTATCAA CCGAAGACGA 
TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG 
GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA 
GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT 
AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAGAG TTCTGTGGAA 
GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA 
TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG 
ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCAGGATC AGTCTATGAC 
ATTTATCTAC CAACACATGT AAGAAAGAAC CCACACTCTA TGATCCAGTG TAGCATCAAA 
CCCCATGCAA TCATCATCCT CCCCAATACA GATGGAATGG AGCTTCTGGT GTGCTATGAA 
GATGAGGGGG TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTAGT TCTACAGTGG 
GGAGAGATGC CTACATCAGT AGCATATATT CGATCCAATC AGACAATGGG CTGGGGAGAG 
AAGGCCATAG AGATCCGATC TGTGGAAACT GGTCACTTGG ATGGTGTGTT CATGCACAAA 
AGGGCTCAAA GACTAAAATT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCTGTT 
CGGTCTGGTG GCAGCAGTCA GGTTTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264119.1
CDS	75..3860
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_005264062.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264062.3

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC 
AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC 
CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA 
GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT 
TTCAGACCCC TCAAGCCTGC TGGCGAAGTG GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC 
TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT 
GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA 
CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
ATTTCTCCAT CTAGCGGAAC AACAGTGACA TCTGTGGTGG GATTTTCCTG TGATGGGATG 
AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT 
ACCAACACTA GGCCACAGAG TGACACCCCG GAGATTCGTA AATACAAGAA GAGGTTTAAC 
TCTGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC 
CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTTATCAA CCGAAGACGA 
TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG 
GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA 
GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT 
AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAGAG TTCTGTGGAA 
GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA 
TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG 
ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCAGGATC AGTCTATGAC 
ATTTATCTAC CAACACATGT AAGAAAGAAC CCACACTCTA TGATCCAGTG TAGCATCAAA 
CCCCATGCAA TCATCATCCT CCCCAATACA GATGGAATGG AGCTTCTGGT GTGCTATGAA 
GATGAGGGGG TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTAGT TCTACAGTGG 
GGAGAGATGC CTACATCAGT AGCATATATT CGATCCAATC AGACAATGGG CTGGGGAGAG 
AAGGCCATAG AGATCCGATC TGTGGAAACT GGTCACTTGG ATGGTGTGTT CATGCACAAA 
AGGGCTCAAA GACTAAAATT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCTGTT 
CGGTCTGGTG GCAGCAGTCA GGTTTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC 
TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_005264063.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264120.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OHu36842
Clone ID Related Accession (Same CDS sequence)	XM_005264063.3
Accession Version	XM_005264063.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264063.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA 
CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT 
GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC 
TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC 
AGACCCCTCA AGCCTGCTGG CGAAGTGGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA 
GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG 
GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA 
GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT 
GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG 
GAGGGCACTC TAATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG 
AGCAATGGCT TTGCCGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC 
TCCTCCACTT CCTCCACCTC CTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC 
CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC 
CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA 
CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC 
AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT 
GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG 
ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT 
CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT 
AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA 
GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA 
GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC 
TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG 
GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC 
TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT 
TATCTACCAA CACATGTAAG AAAGAACCCA CACTCTATGA TCCAGTGTAG CATCAAACCC 
CATGCAATCA TCATCCTCCC CAATACAGAT GGAATGGAGC TTCTGGTGTG CTATGAAGAT 
GAGGGGGTTT ATGTAAACAC ATATGGAAGG ATCACCAAGG ATGTAGTTCT ACAGTGGGGA 
GAGATGCCTA CATCAGTAGC ATATATTCGA TCCAATCAGA CAATGGGCTG GGGAGAGAAG 
GCCATAGAGA TCCGATCTGT GGAAACTGGT CACTTGGATG GTGTGTTCAT GCACAAAAGG 
GCTCAAAGAC TAAAATTCTT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCTGTTCGG 
TCTGGTGGCA GCAGTCAGGT TTATTTCATG ACCTTAGGCA GGACTTCTCT TCTGAGCTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264120.1
CDS	75..3857
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_005264063.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264063.3

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA 
CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT 
GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC 
TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC 
AGACCCCTCA AGCCTGCTGG CGAAGTGGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA 
GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG 
GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA 
GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT 
GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG 
GAGGGCACTC TAATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG 
AGCAATGGCT TTGCCGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC 
TCCTCCACTT CCTCCACCTC CTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC 
CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC 
CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA 
CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC 
AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT 
GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG 
ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT 
CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT 
AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA 
GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA 
GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC 
TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG 
GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC 
TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT 
TATCTACCAA CACATGTAAG AAAGAACCCA CACTCTATGA TCCAGTGTAG CATCAAACCC 
CATGCAATCA TCATCCTCCC CAATACAGAT GGAATGGAGC TTCTGGTGTG CTATGAAGAT 
GAGGGGGTTT ATGTAAACAC ATATGGAAGG ATCACCAAGG ATGTAGTTCT ACAGTGGGGA 
GAGATGCCTA CATCAGTAGC ATATATTCGA TCCAATCAGA CAATGGGCTG GGGAGAGAAG 
GCCATAGAGA TCCGATCTGT GGAAACTGGT CACTTGGATG GTGTGTTCAT GCACAAAAGG 
GCTCAAAGAC TAAAATTCTT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCTGTTCGG 
TCTGGTGGCA GCAGTCAGGT TTATTTCATG ACCTTAGGCA GGACTTCTCT TCTGAGCTGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_005264064.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264121.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X25, mRNA.

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CloneID	OHu36843
Clone ID Related Accession (Same CDS sequence)	XM_005264064.3
Accession Version	XM_005264064.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264064.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC 
AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC 
CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA 
GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT 
TTCAGACCCC TCAAGCCTGC TGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA 
GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG 
ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA 
GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA 
ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC 
ACTCTAATCG TCCGCCAGAG TACAGTTGAC CAAAAGCGTG CCAGCCATCA TGAGAGCAAT 
GGCTTTGCCG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC 
ACTTCCTCCA CCTCCTCCTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG 
ACACCCCAGG ACAAGCTCAC TGCTAATGAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA 
CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
TCTAGCGGAA CAACAGTGAC ATCTGTGGTG GGATTTTCCT GTGATGGGAT GAGACCAGAA 
GCCATAAGGC AAGATCCTAC CCGGAAAGGC TCAGTGGTCA ATGTGAATCC TACCAACACT 
AGGCCACAGA GTGACACCCC GGAGATTCGT AAATACAAGA AGAGGTTTAA CTCTGAGATT 
CTGTGTGCTG CCTTATGGGG AGTGAATTTG CTAGTGGGTA CAGAGAGTGG CCTGATGCTG 
CTGGACAGAA GTGGCCAAGG GAAGGTCTAT CCTCTTATCA ACCGAAGACG ATTTCAACAA 
ATGGACGTAC TTGAGGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA 
CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAATGATCC AGAAGTTGAG 
AAGAAGCAGG GATGGACAAC CGTAGGGGAT TTGGAAGGAT GTGTACATTA TAAAGTTGTA 
AAATATGAAA GAATCAAATT TCTGGTGATT GCTTTGAAGA GTTCTGTGGA AGTCTATGCG 
TGGGCACCAA AGCCATATCA CAAATTTATG GCCTTTAAGT CATTTGGAGA ATTGGTACAT 
AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA 
TCCTGTGCTG GATTCCATGC TGTTGATGTG GATTCAGGAT CAGTCTATGA CATTTATCTA 
CCAACACATG TAAGAAAGAA CCCACACTCT ATGATCCAGT GTAGCATCAA ACCCCATGCA 
ATCATCATCC TCCCCAATAC AGATGGAATG GAGCTTCTGG TGTGCTATGA AGATGAGGGG 
GTTTATGTAA ACACATATGG AAGGATCACC AAGGATGTAG TTCTACAGTG GGGAGAGATG 
CCTACATCAG TAGCATATAT TCGATCCAAT CAGACAATGG GCTGGGGAGA GAAGGCCATA 
GAGATCCGAT CTGTGGAAAC TGGTCACTTG GATGGTGTGT TCATGCACAA AAGGGCTCAA 
AGACTAAAAT TCTTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT 
GGCAGCAGTC AGGTTTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264121.1
CDS	75..3851
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_005264064.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264064.3

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC 
AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC 
CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA 
GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT 
TTCAGACCCC TCAAGCCTGC TGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA 
GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG 
ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA 
GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA 
ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC 
ACTCTAATCG TCCGCCAGAG TACAGTTGAC CAAAAGCGTG CCAGCCATCA TGAGAGCAAT 
GGCTTTGCCG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC 
ACTTCCTCCA CCTCCTCCTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG 
ACACCCCAGG ACAAGCTCAC TGCTAATGAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA 
CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
TCTAGCGGAA CAACAGTGAC ATCTGTGGTG GGATTTTCCT GTGATGGGAT GAGACCAGAA 
GCCATAAGGC AAGATCCTAC CCGGAAAGGC TCAGTGGTCA ATGTGAATCC TACCAACACT 
AGGCCACAGA GTGACACCCC GGAGATTCGT AAATACAAGA AGAGGTTTAA CTCTGAGATT 
CTGTGTGCTG CCTTATGGGG AGTGAATTTG CTAGTGGGTA CAGAGAGTGG CCTGATGCTG 
CTGGACAGAA GTGGCCAAGG GAAGGTCTAT CCTCTTATCA ACCGAAGACG ATTTCAACAA 
ATGGACGTAC TTGAGGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA 
CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAATGATCC AGAAGTTGAG 
AAGAAGCAGG GATGGACAAC CGTAGGGGAT TTGGAAGGAT GTGTACATTA TAAAGTTGTA 
AAATATGAAA GAATCAAATT TCTGGTGATT GCTTTGAAGA GTTCTGTGGA AGTCTATGCG 
TGGGCACCAA AGCCATATCA CAAATTTATG GCCTTTAAGT CATTTGGAGA ATTGGTACAT 
AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA 
TCCTGTGCTG GATTCCATGC TGTTGATGTG GATTCAGGAT CAGTCTATGA CATTTATCTA 
CCAACACATG TAAGAAAGAA CCCACACTCT ATGATCCAGT GTAGCATCAA ACCCCATGCA 
ATCATCATCC TCCCCAATAC AGATGGAATG GAGCTTCTGG TGTGCTATGA AGATGAGGGG 
GTTTATGTAA ACACATATGG AAGGATCACC AAGGATGTAG TTCTACAGTG GGGAGAGATG 
CCTACATCAG TAGCATATAT TCGATCCAAT CAGACAATGG GCTGGGGAGA GAAGGCCATA 
GAGATCCGAT CTGTGGAAAC TGGTCACTTG GATGGTGTGT TCATGCACAA AAGGGCTCAA 
AGACTAAAAT TCTTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT 
GGCAGCAGTC AGGTTTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_005264065.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264122.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OHu36844
Clone ID Related Accession (Same CDS sequence)	XM_005264065.3
Accession Version	XM_005264065.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264065.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG 
AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT 
CTGACCGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TACGGCCACC TCACAAAGTA 
ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG 
GAGCAGGAAG GGGCTGACGA GTCCACCTCA GGACCAGAGG ACACCAGAGC AGCGTCATCT 
CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA 
GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGAGTACA 
GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCCGGTCG CATTCACCTC 
TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCCTCCCCA 
TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT 
AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GCGGAACAAC AGTGACATCT 
GTGGTGGGAT TTTCCTGTGA TGGGATGAGA CCAGAAGCCA TAAGGCAAGA TCCTACCCGG 
AAAGGCTCAG TGGTCAATGT GAATCCTACC AACACTAGGC CACAGAGTGA CACCCCGGAG 
ATTCGTAAAT ACAAGAAGAG GTTTAACTCT GAGATTCTGT GTGCTGCCTT ATGGGGAGTG 
AATTTGCTAG TGGGTACAGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
GTCTATCCTC TTATCAACCG AAGACGATTT CAACAAATGG ACGTACTTGA GGGCTTGAAT 
GTCTTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
AGAAATAAAA TACTTCACAA TGATCCAGAA GTTGAGAAGA AGCAGGGATG GACAACCGTA 
GGGGATTTGG AAGGATGTGT ACATTATAAA GTTGTAAAAT ATGAAAGAAT CAAATTTCTG 
GTGATTGCTT TGAAGAGTTC TGTGGAAGTC TATGCGTGGG CACCAAAGCC ATATCACAAA 
TTTATGGCCT TTAAGTCATT TGGAGAATTG GTACATAAGC CATTACTGGT GGATCTCACT 
GTTGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
GATGTGGATT CAGGATCAGT CTATGACATT TATCTACCAA CACATGTAAG AAAGAACCCA 
CACTCTATGA TCCAGTGTAG CATCAAACCC CATGCAATCA TCATCCTCCC CAATACAGAT 
GGAATGGAGC TTCTGGTGTG CTATGAAGAT GAGGGGGTTT ATGTAAACAC ATATGGAAGG 
ATCACCAAGG ATGTAGTTCT ACAGTGGGGA GAGATGCCTA CATCAGTAGC ATATATTCGA 
TCCAATCAGA CAATGGGCTG GGGAGAGAAG GCCATAGAGA TCCGATCTGT GGAAACTGGT 
CACTTGGATG GTGTGTTCAT GCACAAAAGG GCTCAAAGAC TAAAATTCTT GTGTGAACGC 
AATGACAAGG TGTTCTTTGC CTCTGTTCGG TCTGGTGGCA GCAGTCAGGT TTATTTCATG 
ACCTTAGGCA GGACTTCTCT TCTGAGCTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264122.1
CDS	75..3767
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_005264065.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264065.3

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG 
AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT 
CTGACCGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TACGGCCACC TCACAAAGTA 
ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG 
GAGCAGGAAG GGGCTGACGA GTCCACCTCA GGACCAGAGG ACACCAGAGC AGCGTCATCT 
CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA 
GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGAGTACA 
GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCCGGTCG CATTCACCTC 
TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCCTCCCCA 
TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT 
AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GCGGAACAAC AGTGACATCT 
GTGGTGGGAT TTTCCTGTGA TGGGATGAGA CCAGAAGCCA TAAGGCAAGA TCCTACCCGG 
AAAGGCTCAG TGGTCAATGT GAATCCTACC AACACTAGGC CACAGAGTGA CACCCCGGAG 
ATTCGTAAAT ACAAGAAGAG GTTTAACTCT GAGATTCTGT GTGCTGCCTT ATGGGGAGTG 
AATTTGCTAG TGGGTACAGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
GTCTATCCTC TTATCAACCG AAGACGATTT CAACAAATGG ACGTACTTGA GGGCTTGAAT 
GTCTTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
AGAAATAAAA TACTTCACAA TGATCCAGAA GTTGAGAAGA AGCAGGGATG GACAACCGTA 
GGGGATTTGG AAGGATGTGT ACATTATAAA GTTGTAAAAT ATGAAAGAAT CAAATTTCTG 
GTGATTGCTT TGAAGAGTTC TGTGGAAGTC TATGCGTGGG CACCAAAGCC ATATCACAAA 
TTTATGGCCT TTAAGTCATT TGGAGAATTG GTACATAAGC CATTACTGGT GGATCTCACT 
GTTGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
GATGTGGATT CAGGATCAGT CTATGACATT TATCTACCAA CACATGTAAG AAAGAACCCA 
CACTCTATGA TCCAGTGTAG CATCAAACCC CATGCAATCA TCATCCTCCC CAATACAGAT 
GGAATGGAGC TTCTGGTGTG CTATGAAGAT GAGGGGGTTT ATGTAAACAC ATATGGAAGG 
ATCACCAAGG ATGTAGTTCT ACAGTGGGGA GAGATGCCTA CATCAGTAGC ATATATTCGA 
TCCAATCAGA CAATGGGCTG GGGAGAGAAG GCCATAGAGA TCCGATCTGT GGAAACTGGT 
CACTTGGATG GTGTGTTCAT GCACAAAAGG GCTCAAAGAC TAAAATTCTT GTGTGAACGC 
AATGACAAGG TGTTCTTTGC CTCTGTTCGG TCTGGTGGCA GCAGTCAGGT TTATTTCATG 
ACCTTAGGCA GGACTTCTCT TCTGAGCTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_005264066.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264123.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OHu36845
Clone ID Related Accession (Same CDS sequence)	XM_005264066.3
Accession Version	XM_005264066.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264066.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGTA TTGAGCCCAG GCTTCTGTGG 
GAGAGAGTGG AGAAGCTGGT GCCCAGACCT GGCAGTGGCA GCTCCTCAGG GTCCAGCAAC 
TCAGGATCCC AGCCCGGGTC TCACCCTGGG TCTCAGAGTG GCTCCGGGGA ACGCTTCAGA 
GTGAGATCAT CATCCAAGTC TGAAGGCTCT CCATCTCAGC GCCTGGAAAA TGCAGTGAAA 
AAACCTGAAG ATAAAAAGGA AGTTTTCAGA CCCCTCAAGC CTGCTGGCGA AGTGGATCTG 
ACCGCACTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTAC GGCCACCTCA CAAAGTAACG 
GACTACTCCT CATCCAGTGA GGAGTCGGGG ACGACGGATG AGGAGGACGA CGATGTGGAG 
CAGGAAGGGG CTGACGAGTC CACCTCAGGA CCAGAGGACA CCAGAGCAGC GTCATCTCTG 
AATTTGAGCA ATGGTGAAAC GGAATCTGTG AAAACCATGA TTGTCCATGA TGATGTAGAA 
AGTGAGCCGG CCATGACCCC ATCCAAGGAG GGCACTCTAA TCGTCCGCCA GAGTACAGTT 
GACCAAAAGC GTGCCAGCCA TCATGAGAGC AATGGCTTTG CCGGTCGCAT TCACCTCTTG 
CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCCTC CTCCCCATCC 
TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTGCTAAT 
GAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGCG GAACAACAGT GACATCTGTG 
GTGGGATTTT CCTGTGATGG GATGAGACCA GAAGCCATAA GGCAAGATCC TACCCGGAAA 
GGCTCAGTGG TCAATGTGAA TCCTACCAAC ACTAGGCCAC AGAGTGACAC CCCGGAGATT 
CGTAAATACA AGAAGAGGTT TAACTCTGAG ATTCTGTGTG CTGCCTTATG GGGAGTGAAT 
TTGCTAGTGG GTACAGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTC 
TATCCTCTTA TCAACCGAAG ACGATTTCAA CAAATGGACG TACTTGAGGG CTTGAATGTC 
TTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA 
AATAAAATAC TTCACAATGA TCCAGAAGTT GAGAAGAAGC AGGGATGGAC AACCGTAGGG 
GATTTGGAAG GATGTGTACA TTATAAAGTT GTAAAATATG AAAGAATCAA ATTTCTGGTG 
ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT GCGTGGGCAC CAAAGCCATA TCACAAATTT 
ATGGCCTTTA AGTCATTTGG AGAATTGGTA CATAAGCCAT TACTGGTGGA TCTCACTGTT 
GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT 
GTGGATTCAG GATCAGTCTA TGACATTTAT CTACCAACAC ATGTAAGAAA GAACCCACAC 
TCTATGATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA 
ATGGAGCTTC TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC 
ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACAT CAGTAGCATA TATTCGATCC 
AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC 
TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT 
GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC 
TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264123.1
CDS	75..3764
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_005264066.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264066.3

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGTA TTGAGCCCAG GCTTCTGTGG 
GAGAGAGTGG AGAAGCTGGT GCCCAGACCT GGCAGTGGCA GCTCCTCAGG GTCCAGCAAC 
TCAGGATCCC AGCCCGGGTC TCACCCTGGG TCTCAGAGTG GCTCCGGGGA ACGCTTCAGA 
GTGAGATCAT CATCCAAGTC TGAAGGCTCT CCATCTCAGC GCCTGGAAAA TGCAGTGAAA 
AAACCTGAAG ATAAAAAGGA AGTTTTCAGA CCCCTCAAGC CTGCTGGCGA AGTGGATCTG 
ACCGCACTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTAC GGCCACCTCA CAAAGTAACG 
GACTACTCCT CATCCAGTGA GGAGTCGGGG ACGACGGATG AGGAGGACGA CGATGTGGAG 
CAGGAAGGGG CTGACGAGTC CACCTCAGGA CCAGAGGACA CCAGAGCAGC GTCATCTCTG 
AATTTGAGCA ATGGTGAAAC GGAATCTGTG AAAACCATGA TTGTCCATGA TGATGTAGAA 
AGTGAGCCGG CCATGACCCC ATCCAAGGAG GGCACTCTAA TCGTCCGCCA GAGTACAGTT 
GACCAAAAGC GTGCCAGCCA TCATGAGAGC AATGGCTTTG CCGGTCGCAT TCACCTCTTG 
CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCCTC CTCCCCATCC 
TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTGCTAAT 
GAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGCG GAACAACAGT GACATCTGTG 
GTGGGATTTT CCTGTGATGG GATGAGACCA GAAGCCATAA GGCAAGATCC TACCCGGAAA 
GGCTCAGTGG TCAATGTGAA TCCTACCAAC ACTAGGCCAC AGAGTGACAC CCCGGAGATT 
CGTAAATACA AGAAGAGGTT TAACTCTGAG ATTCTGTGTG CTGCCTTATG GGGAGTGAAT 
TTGCTAGTGG GTACAGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTC 
TATCCTCTTA TCAACCGAAG ACGATTTCAA CAAATGGACG TACTTGAGGG CTTGAATGTC 
TTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA 
AATAAAATAC TTCACAATGA TCCAGAAGTT GAGAAGAAGC AGGGATGGAC AACCGTAGGG 
GATTTGGAAG GATGTGTACA TTATAAAGTT GTAAAATATG AAAGAATCAA ATTTCTGGTG 
ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT GCGTGGGCAC CAAAGCCATA TCACAAATTT 
ATGGCCTTTA AGTCATTTGG AGAATTGGTA CATAAGCCAT TACTGGTGGA TCTCACTGTT 
GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT 
GTGGATTCAG GATCAGTCTA TGACATTTAT CTACCAACAC ATGTAAGAAA GAACCCACAC 
TCTATGATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA 
ATGGAGCTTC TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC 
ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACAT CAGTAGCATA TATTCGATCC 
AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC 
TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT 
GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC 
TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_005264068.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264125.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OHu36846
Clone ID Related Accession (Same CDS sequence)	XM_005264068.3
Accession Version	XM_005264068.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264068.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG 
AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGA TCTGACCGCA 
CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC 
TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA 
GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG 
AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG 
CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGAGTAC AGTTGACCAA 
AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCCGGTC GCATTCACCT CTTGCCAGAT 
CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCCTCCCC ATCCTCCAGC 
CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT 
CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA 
TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA 
GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA 
TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA 
GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT 
CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG 
ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG 
GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT 
TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC 
TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA 
GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT 
TCAGGATCAG TCTATGACAT TTATCTACCA ACACATGTAA GAAAGAACCC ACACTCTATG 
ATCCAGTGTA GCATCAAACC CCATGCAATC ATCATCCTCC CCAATACAGA TGGAATGGAG 
CTTCTGGTGT GCTATGAAGA TGAGGGGGTT TATGTAAACA CATATGGAAG GATCACCAAG 
GATGTAGTTC TACAGTGGGG AGAGATGCCT ACATCAGTAG CATATATTCG ATCCAATCAG 
ACAATGGGCT GGGGAGAGAA GGCCATAGAG ATCCGATCTG TGGAAACTGG TCACTTGGAT 
GGTGTGTTCA TGCACAAAAG GGCTCAAAGA CTAAAATTCT TGTGTGAACG CAATGACAAG 
GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC AGCAGTCAGG TTTATTTCAT GACCTTAGGC 
AGGACTTCTC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264125.1
CDS	75..3758
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_005264068.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264068.3

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG 
AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGA TCTGACCGCA 
CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC 
TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA 
GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG 
AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG 
CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGAGTAC AGTTGACCAA 
AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCCGGTC GCATTCACCT CTTGCCAGAT 
CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCCTCCCC ATCCTCCAGC 
CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT 
CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA 
TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA 
GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA 
TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA 
GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT 
CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG 
ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG 
GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT 
TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC 
TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA 
GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT 
TCAGGATCAG TCTATGACAT TTATCTACCA ACACATGTAA GAAAGAACCC ACACTCTATG 
ATCCAGTGTA GCATCAAACC CCATGCAATC ATCATCCTCC CCAATACAGA TGGAATGGAG 
CTTCTGGTGT GCTATGAAGA TGAGGGGGTT TATGTAAACA CATATGGAAG GATCACCAAG 
GATGTAGTTC TACAGTGGGG AGAGATGCCT ACATCAGTAG CATATATTCG ATCCAATCAG 
ACAATGGGCT GGGGAGAGAA GGCCATAGAG ATCCGATCTG TGGAAACTGG TCACTTGGAT 
GGTGTGTTCA TGCACAAAAG GGCTCAAAGA CTAAAATTCT TGTGTGAACG CAATGACAAG 
GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC AGCAGTCAGG TTTATTTCAT GACCTTAGGC 
AGGACTTCTC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_005264069.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264126.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X35, mRNA.

Price & Availability↑

CloneID	OHu36847
Clone ID Related Accession (Same CDS sequence)	XM_005264069.2
Accession Version	XM_005264069.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264069.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA 
AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC 
TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC 
AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
AGCGGAACAA CAGTGACATC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCAGAAGCC 
ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG 
CCACAGAGTG ACACCCCGGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAGATTCTG 
TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG 
GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTTATCAACC GAAGACGATT TCAACAAATG 
GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT 
GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG 
AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA 
TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCGTGG 
GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG 
CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC 
TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCAGGATCAG TCTATGACAT TTATCTACCA 
ACACATATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA 
ATGGAGCTTC TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC 
ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACAT CAGTAGCATA TATTCGATCC 
AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC 
TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT 
GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC 
TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264126.1
CDS	75..3704
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_005264069.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264069.2

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT 
AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA 
TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG 
CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC 
CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA 
GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG 
CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC 
GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC 
AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA 
AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC 
TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC 
AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
AGCGGAACAA CAGTGACATC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCAGAAGCC 
ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG 
CCACAGAGTG ACACCCCGGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAGATTCTG 
TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG 
GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTTATCAACC GAAGACGATT TCAACAAATG 
GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT 
GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG 
AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA 
TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCGTGG 
GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG 
CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC 
TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCAGGATCAG TCTATGACAT TTATCTACCA 
ACACATATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA 
ATGGAGCTTC TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC 
ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACAT CAGTAGCATA TATTCGATCC 
AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC 
TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT 
GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC 
TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_005264071.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264128.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X40, mRNA.

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CloneID	OHu36848
Clone ID Related Accession (Same CDS sequence)	XM_005264071.2
Accession Version	XM_005264071.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X40
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264071.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA 
CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT 
GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC 
TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC 
AGACCCCTCA AGCCTGCTGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT 
GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG 
GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG 
GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC 
ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT 
CTAATCGTCC GCCAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC 
TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGAACAACA 
GTGACATCTG TGGTGGGATT TTCCTGTGAT GGGATGAGAC CAGAAGCCAT AAGGCAAGAT 
CCTACCCGGA AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAC 
ACCCCGGAGA TTCGTAAATA CAAGAAGAGG TTTAACTCTG AGATTCTGTG TGCTGCCTTA 
TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC 
CAAGGGAAGG TCTATCCTCT TATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG 
GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG 
TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG 
ACAACCGTAG GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC 
AAATTTCTGG TGATTGCTTT GAAGAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA 
TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG 
GATCTCACTG TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC 
CATGCTGTTG ATGTGGATTC AGGATCAGTC TATGACATTT ATCTACCAAC ACATATCCAG 
TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG 
GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA 
GTTCTACAGT GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG 
GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG 
TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC 
TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT 
TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264128.1
CDS	75..3632
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPADLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_005264071.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X40, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264071.2

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA 
CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT 
GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC 
TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC 
AGACCCCTCA AGCCTGCTGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT 
GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG 
GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG 
GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC 
ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT 
CTAATCGTCC GCCAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC 
TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGAACAACA 
GTGACATCTG TGGTGGGATT TTCCTGTGAT GGGATGAGAC CAGAAGCCAT AAGGCAAGAT 
CCTACCCGGA AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAC 
ACCCCGGAGA TTCGTAAATA CAAGAAGAGG TTTAACTCTG AGATTCTGTG TGCTGCCTTA 
TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC 
CAAGGGAAGG TCTATCCTCT TATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG 
GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG 
TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG 
ACAACCGTAG GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC 
AAATTTCTGG TGATTGCTTT GAAGAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA 
TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG 
GATCTCACTG TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC 
CATGCTGTTG ATGTGGATTC AGGATCAGTC TATGACATTT ATCTACCAAC ACATATCCAG 
TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG 
GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA 
GTTCTACAGT GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG 
GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG 
TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC 
TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT 
TCTCTTCTGA GCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_006712868.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712931.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X45, mRNA.

Price & Availability↑

CloneID	OHu36850
Clone ID Related Accession (Same CDS sequence)	XM_006712868.2
Accession Version	XM_006712868.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X45
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006712868.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG 
AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT 
CTGACCGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TACGGCCACC TCACAAAGTA 
ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG 
GAGCAGGAAG GGGCTGACGA GTCCACCTCA GGACCAGAGG ACACCAGAGC AGCGTCATCT 
CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA 
GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGAGTACA 
GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCCGGTCG CATTCACCTC 
TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCCTCCCCA 
TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT 
AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GCGGAACAAC AGTGACATCT 
GTGGTGGGAT TTTCCTGTGA TGGGATGAGA CCAGAAGCCA TAAGGCAAGA TCCTACCCGG 
AAAGGCTCAG TGGTCAATGT GAATCCTACC AACACTAGGC CACAGAGTGA CACCCCGGAG 
ATTCGTAAAT ACAAGAAGAG GTTTAACTCT GAGATTCTGT GTGCTGCCTT ATGGGGAGTG 
AATTTGCTAG TGGGTACAGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
GTCTATCCTC TTATCAACCG AAGACGATTT CAACAAATGG ACGTACTTGA GGGCTTGAAT 
GTCTTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
AGAAATAAAA TACTTCACAA TGATCCAGAA GTTGAGAAGA AGCAGGGATG GACAACCGTA 
GGGGATTTGG AAGGATGTGT ACATTATAAA GTTGTAAAAT ATGAAAGAAT CAAATTTCTG 
GTGATTGCTT TGAAGAGTTC TGTGGAAGTC TATGCGTGGG CACCAAAGCC ATATCACAAA 
TTTATGGCCT TTAAGTCATT TGGAGAATTG GTACATAAGC CATTACTGGT GGATCTCACT 
GTTGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
GATGTGGATT CAGGATCAGT CTATGACATT TATCTACCAA CACATATCCA GTGTAGCATC 
AAACCCCATG CAATCATCAT CCTCCCCAAT ACAGATGGAA TGGAGCTTCT GGTGTGCTAT 
GAAGATGAGG GGGTTTATGT AAACACATAT GGAAGGATCA CCAAGGATGT AGTTCTACAG 
TGGGGAGAGA TGCCTACATC AGTAGCATAT ATTCGATCCA ATCAGACAAT GGGCTGGGGA 
GAGAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGTCACT TGGATGGTGT GTTCATGCAC 
AAAAGGGCTC AAAGACTAAA ATTCTTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCT 
GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT TTCATGACCT TAGGCAGGAC TTCTCTTCTG 
AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712931.1
CDS	75..3743
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_006712868.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X45, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712868.2

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG 
CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC 
CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG 
AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG 
CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG 
TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC 
AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC 
AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG 
AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT 
CTGACCGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TACGGCCACC TCACAAAGTA 
ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG 
GAGCAGGAAG GGGCTGACGA GTCCACCTCA GGACCAGAGG ACACCAGAGC AGCGTCATCT 
CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA 
GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGAGTACA 
GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCCGGTCG CATTCACCTC 
TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCCTCCCCA 
TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT 
AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GCGGAACAAC AGTGACATCT 
GTGGTGGGAT TTTCCTGTGA TGGGATGAGA CCAGAAGCCA TAAGGCAAGA TCCTACCCGG 
AAAGGCTCAG TGGTCAATGT GAATCCTACC AACACTAGGC CACAGAGTGA CACCCCGGAG 
ATTCGTAAAT ACAAGAAGAG GTTTAACTCT GAGATTCTGT GTGCTGCCTT ATGGGGAGTG 
AATTTGCTAG TGGGTACAGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
GTCTATCCTC TTATCAACCG AAGACGATTT CAACAAATGG ACGTACTTGA GGGCTTGAAT 
GTCTTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
AGAAATAAAA TACTTCACAA TGATCCAGAA GTTGAGAAGA AGCAGGGATG GACAACCGTA 
GGGGATTTGG AAGGATGTGT ACATTATAAA GTTGTAAAAT ATGAAAGAAT CAAATTTCTG 
GTGATTGCTT TGAAGAGTTC TGTGGAAGTC TATGCGTGGG CACCAAAGCC ATATCACAAA 
TTTATGGCCT TTAAGTCATT TGGAGAATTG GTACATAAGC CATTACTGGT GGATCTCACT 
GTTGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
GATGTGGATT CAGGATCAGT CTATGACATT TATCTACCAA CACATATCCA GTGTAGCATC 
AAACCCCATG CAATCATCAT CCTCCCCAAT ACAGATGGAA TGGAGCTTCT GGTGTGCTAT 
GAAGATGAGG GGGTTTATGT AAACACATAT GGAAGGATCA CCAAGGATGT AGTTCTACAG 
TGGGGAGAGA TGCCTACATC AGTAGCATAT ATTCGATCCA ATCAGACAAT GGGCTGGGGA 
GAGAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGTCACT TGGATGGTGT GTTCATGCAC 
AAAAGGGCTC AAAGACTAAA ATTCTTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCT 
GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT TTCATGACCT TAGGCAGGAC TTCTCTTCTG 
AGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_006712869.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712932.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X37, mRNA.

Price & Availability↑

CloneID	OHu36851
Clone ID Related Accession (Same CDS sequence)	XM_006712869.2
Accession Version	XM_006712869.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X37
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006712869.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC 
AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC 
CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA 
GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT 
TTCAGACCCC TCAAGCCTGC TGGCGAAGTG GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC 
TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC 
AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
AGCGGAACAA CAGTGACATC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCAGAAGCC 
ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG 
CCACAGAGTG ACACCCCGGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAGATTCTG 
TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG 
GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTTATCAACC GAAGACGATT TCAACAAATG 
GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT 
GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG 
AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA 
TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCGTGG 
GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG 
CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC 
TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCAGGATCAG TCTATGACAT TTATCTACCA 
ACACATATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA 
ATGGAGCTTC TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC 
ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACAT CAGTAGCATA TATTCGATCC 
AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC 
TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT 
GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC 
TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712932.1
CDS	75..3644
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_006712869.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712869.2

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG 
TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA 
AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC 
AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC 
CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA 
GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT 
TTCAGACCCC TCAAGCCTGC TGGCGAAGTG GATCTGACCG CACTGGCCAA AGAGCTTCGA 
GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG 
TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC 
TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA 
TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC 
AAGGAGGGCA CTCTAATCGT CCGCCAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC 
AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
AGCGGAACAA CAGTGACATC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCAGAAGCC 
ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG 
CCACAGAGTG ACACCCCGGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAGATTCTG 
TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG 
GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTTATCAACC GAAGACGATT TCAACAAATG 
GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT 
GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG 
AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA 
TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCGTGG 
GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG 
CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC 
TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCAGGATCAG TCTATGACAT TTATCTACCA 
ACACATATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA 
ATGGAGCTTC TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC 
ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACAT CAGTAGCATA TATTCGATCC 
AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC 
TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT 
GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC 
TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_005264048.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264105.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.

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CloneID	OHu36825
Clone ID Related Accession (Same CDS sequence)	XM_005264048.2
Accession Version	XM_005264048.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264048.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTAC AGTGGTCCCA CCTGGCATCT CTCAAGAACA ATGTTTCCCC TGTCTCGCGA 
TCCCATTCCT TCAGTGACCC TTCTCCCAAA TTTGCACACC ACCATCTTCG TTCTCAGGAC 
CCATGTCCAC CTTCCCGCAG TGAGGTGCTC AGTCAGAGCT CTGACTCTAA GTCAGAGGCG 
CCTGACCCTA CCCAAAAGGC TTGGTCTAGA TCAGACAGTG ACGAGGTGCC TCCAAGGGTT 
CCTGTGAGAA CAACATCTCG CTCCCCTGTT CTGTCCCGTC GAGATTCCCC ACTGCAGGGC 
AGTGGGCAGC AGAATAGCCA GGCAGGACAG AGAAACTCCA CCAGCAGTAT TGAGCCCAGG 
CTTCTGTGGG AGAGAGTGGA GAAGCTGGTG CCCAGACCTG GCAGTGGCAG CTCCTCAGGG 
TCCAGCAACT CAGGATCCCA GCCCGGGTCT CACCCTGGGT CTCAGAGTGG CTCCGGGGAA 
CGCTTCAGAG TGAGATCATC ATCCAAGTCT GAAGGCTCTC CATCTCAGCG CCTGGAAAAT 
GCAGTGAAAA AACCTGAAGA TAAAAAGGAA GTTTTCAGAC CCCTCAAGCC TGCTGGCGAA 
GTGGATCTGA CCGCACTGGC CAAAGAGCTT CGAGCAGTGG AAGATGTACG GCCACCTCAC 
AAAGTAACGG ACTACTCCTC ATCCAGTGAG GAGTCGGGGA CGACGGATGA GGAGGACGAC 
GATGTGGAGC AGGAAGGGGC TGACGAGTCC ACCTCAGGAC CAGAGGACAC CAGAGCAGCG 
TCATCTCTGA ATTTGAGCAA TGGTGAAACG GAATCTGTGA AAACCATGAT TGTCCATGAT 
GATGTAGAAA GTGAGCCGGC CATGACCCCA TCCAAGGAGG GCACTCTAAT CGTCCGCCAG 
AGTACAGTTG ACCAAAAGCG TGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT 
CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC 
TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC 
ACTGCTAATG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC 
TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGCGG AACAACAGTG 
ACATCTGTGG TGGGATTTTC CTGTGATGGG ATGAGACCAG AAGCCATAAG GCAAGATCCT 
ACCCGGAAAG GCTCAGTGGT CAATGTGAAT CCTACCAACA CTAGGCCACA GAGTGACACC 
CCGGAGATTC GTAAATACAA GAAGAGGTTT AACTCTGAGA TTCTGTGTGC TGCCTTATGG 
GGAGTGAATT TGCTAGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA 
GGGAAGGTCT ATCCTCTTAT CAACCGAAGA CGATTTCAAC AAATGGACGT ACTTGAGGGC 
TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC 
TGGTTAAGAA ATAAAATACT TCACAATGAT CCAGAAGTTG AGAAGAAGCA GGGATGGACA 
ACCGTAGGGG ATTTGGAAGG ATGTGTACAT TATAAAGTTG TAAAATATGA AAGAATCAAA 
TTTCTGGTGA TTGCTTTGAA GAGTTCTGTG GAAGTCTATG CGTGGGCACC AAAGCCATAT 
CACAAATTTA TGGCCTTTAA GTCATTTGGA GAATTGGTAC ATAAGCCATT ACTGGTGGAT 
CTCACTGTTG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT 
GCTGTTGATG TGGATTCAGG ATCAGTCTAT GACATTTATC TACCAACACA TATCCAGTGT 
AGCATCAAAC CCCATGCAAT CATCATCCTC CCCAATACAG ATGGAATGGA GCTTCTGGTG 
TGCTATGAAG ATGAGGGGGT TTATGTAAAC ACATATGGAA GGATCACCAA GGATGTAGTT 
CTACAGTGGG GAGAGATGCC TACATCAGTA GCATATATTC GATCCAATCA GACAATGGGC 
TGGGGAGAGA AGGCCATAGA GATCCGATCT GTGGAAACTG GTCACTTGGA TGGTGTGTTC 
ATGCACAAAA GGGCTCAAAG ACTAAAATTC TTGTGTGAAC GCAATGACAA GGTGTTCTTT 
GCCTCTGTTC GGTCTGGTGG CAGCAGTCAG GTTTATTTCA TGACCTTAGG CAGGACTTCT 
CTTCTGAGCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264105.1
CDS	75..4229
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_005264048.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264048.2

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC 
CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA 
GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG 
CCACAGGTAC AGTGGTCCCA CCTGGCATCT CTCAAGAACA ATGTTTCCCC TGTCTCGCGA 
TCCCATTCCT TCAGTGACCC TTCTCCCAAA TTTGCACACC ACCATCTTCG TTCTCAGGAC 
CCATGTCCAC CTTCCCGCAG TGAGGTGCTC AGTCAGAGCT CTGACTCTAA GTCAGAGGCG 
CCTGACCCTA CCCAAAAGGC TTGGTCTAGA TCAGACAGTG ACGAGGTGCC TCCAAGGGTT 
CCTGTGAGAA CAACATCTCG CTCCCCTGTT CTGTCCCGTC GAGATTCCCC ACTGCAGGGC 
AGTGGGCAGC AGAATAGCCA GGCAGGACAG AGAAACTCCA CCAGCAGTAT TGAGCCCAGG 
CTTCTGTGGG AGAGAGTGGA GAAGCTGGTG CCCAGACCTG GCAGTGGCAG CTCCTCAGGG 
TCCAGCAACT CAGGATCCCA GCCCGGGTCT CACCCTGGGT CTCAGAGTGG CTCCGGGGAA 
CGCTTCAGAG TGAGATCATC ATCCAAGTCT GAAGGCTCTC CATCTCAGCG CCTGGAAAAT 
GCAGTGAAAA AACCTGAAGA TAAAAAGGAA GTTTTCAGAC CCCTCAAGCC TGCTGGCGAA 
GTGGATCTGA CCGCACTGGC CAAAGAGCTT CGAGCAGTGG AAGATGTACG GCCACCTCAC 
AAAGTAACGG ACTACTCCTC ATCCAGTGAG GAGTCGGGGA CGACGGATGA GGAGGACGAC 
GATGTGGAGC AGGAAGGGGC TGACGAGTCC ACCTCAGGAC CAGAGGACAC CAGAGCAGCG 
TCATCTCTGA ATTTGAGCAA TGGTGAAACG GAATCTGTGA AAACCATGAT TGTCCATGAT 
GATGTAGAAA GTGAGCCGGC CATGACCCCA TCCAAGGAGG GCACTCTAAT CGTCCGCCAG 
AGTACAGTTG ACCAAAAGCG TGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT 
CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC 
TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC 
ACTGCTAATG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC 
TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGCGG AACAACAGTG 
ACATCTGTGG TGGGATTTTC CTGTGATGGG ATGAGACCAG AAGCCATAAG GCAAGATCCT 
ACCCGGAAAG GCTCAGTGGT CAATGTGAAT CCTACCAACA CTAGGCCACA GAGTGACACC 
CCGGAGATTC GTAAATACAA GAAGAGGTTT AACTCTGAGA TTCTGTGTGC TGCCTTATGG 
GGAGTGAATT TGCTAGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA 
GGGAAGGTCT ATCCTCTTAT CAACCGAAGA CGATTTCAAC AAATGGACGT ACTTGAGGGC 
TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC 
TGGTTAAGAA ATAAAATACT TCACAATGAT CCAGAAGTTG AGAAGAAGCA GGGATGGACA 
ACCGTAGGGG ATTTGGAAGG ATGTGTACAT TATAAAGTTG TAAAATATGA AAGAATCAAA 
TTTCTGGTGA TTGCTTTGAA GAGTTCTGTG GAAGTCTATG CGTGGGCACC AAAGCCATAT 
CACAAATTTA TGGCCTTTAA GTCATTTGGA GAATTGGTAC ATAAGCCATT ACTGGTGGAT 
CTCACTGTTG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT 
GCTGTTGATG TGGATTCAGG ATCAGTCTAT GACATTTATC TACCAACACA TATCCAGTGT 
AGCATCAAAC CCCATGCAAT CATCATCCTC CCCAATACAG ATGGAATGGA GCTTCTGGTG 
TGCTATGAAG ATGAGGGGGT TTATGTAAAC ACATATGGAA GGATCACCAA GGATGTAGTT 
CTACAGTGGG GAGAGATGCC TACATCAGTA GCATATATTC GATCCAATCA GACAATGGGC 
TGGGGAGAGA AGGCCATAGA GATCCGATCT GTGGAAACTG GTCACTTGGA TGGTGTGTTC 
ATGCACAAAA GGGCTCAAAG ACTAAAATTC TTGTGTGAAC GCAATGACAA GGTGTTCTTT 
GCCTCTGTTC GGTCTGGTGG CAGCAGTCAG GTTTATTTCA TGACCTTAGG CAGGACTTCT 
CTTCTGAGCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_005264050.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264107.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu36828
Clone ID Related Accession (Same CDS sequence)	XM_005264050.3
Accession Version	XM_005264050.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264050.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC 
CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT 
GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC 
GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA 
GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC 
AGCAGTATTG AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC 
AGTGGCAGCT CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT 
CAGAGTGGCT CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA 
TCTCAGCGCC TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC 
CTCAAGCCTG CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA 
GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG 
ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA 
GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA 
ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC 
ACTCTAATCG TCCGCCAGAG TACAGTTGAC CAAAAGCGTG CCAGCCATCA TGAGAGCAAT 
GGCTTTGCCG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC 
ACTTCCTCCA CCTCCTCCTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG 
ACACCCCAGG ACAAGCTCAC TGCTAATGAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA 
CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
TCTAGCGGAA CAACAGTGAC ATCTGTGGTG GGATTTTCCT GTGATGGGAT GAGACCAGAA 
GCCATAAGGC AAGATCCTAC CCGGAAAGGC TCAGTGGTCA ATGTGAATCC TACCAACACT 
AGGCCACAGA GTGACACCCC GGAGATTCGT AAATACAAGA AGAGGTTTAA CTCTGAGATT 
CTGTGTGCTG CCTTATGGGG AGTGAATTTG CTAGTGGGTA CAGAGAGTGG CCTGATGCTG 
CTGGACAGAA GTGGCCAAGG GAAGGTCTAT CCTCTTATCA ACCGAAGACG ATTTCAACAA 
ATGGACGTAC TTGAGGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA 
CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAATGATCC AGAAGTTGAG 
AAGAAGCAGG GATGGACAAC CGTAGGGGAT TTGGAAGGAT GTGTACATTA TAAAGTTGTA 
AAATATGAAA GAATCAAATT TCTGGTGATT GCTTTGAAGA GTTCTGTGGA AGTCTATGCG 
TGGGCACCAA AGCCATATCA CAAATTTATG GCCTTTAAGT CATTTGGAGA ATTGGTACAT 
AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA 
TCCTGTGCTG GATTCCATGC TGTTGATGTG GATTCAGGAT CAGTCTATGA CATTTATCTA 
CCAACACATG TAAGAAAGAA CCCACACTCT ATGATCCAGT GTAGCATCAA ACCCCATGCA 
ATCATCATCC TCCCCAATAC AGATGGAATG GAGCTTCTGG TGTGCTATGA AGATGAGGGG 
GTTTATGTAA ACACATATGG AAGGATCACC AAGGATGTAG TTCTACAGTG GGGAGAGATG 
CCTACATCAG TAGCATATAT TCGATCCAAT CAGACAATGG GCTGGGGAGA GAAGGCCATA 
GAGATCCGAT CTGTGGAAAC TGGTCACTTG GATGGTGTGT TCATGCACAA AAGGGCTCAA 
AGACTAAAAT TCTTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT 
GGCAGCAGTC AGGTTTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264107.1
CDS	75..4091
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_005264050.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264050.3

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC 
CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT 
GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC 
GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA 
GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC 
AGCAGTATTG AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC 
AGTGGCAGCT CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT 
CAGAGTGGCT CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA 
TCTCAGCGCC TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC 
CTCAAGCCTG CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA 
GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG 
ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA 
GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA 
ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC 
ACTCTAATCG TCCGCCAGAG TACAGTTGAC CAAAAGCGTG CCAGCCATCA TGAGAGCAAT 
GGCTTTGCCG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC 
ACTTCCTCCA CCTCCTCCTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG 
ACACCCCAGG ACAAGCTCAC TGCTAATGAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA 
CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
TCTAGCGGAA CAACAGTGAC ATCTGTGGTG GGATTTTCCT GTGATGGGAT GAGACCAGAA 
GCCATAAGGC AAGATCCTAC CCGGAAAGGC TCAGTGGTCA ATGTGAATCC TACCAACACT 
AGGCCACAGA GTGACACCCC GGAGATTCGT AAATACAAGA AGAGGTTTAA CTCTGAGATT 
CTGTGTGCTG CCTTATGGGG AGTGAATTTG CTAGTGGGTA CAGAGAGTGG CCTGATGCTG 
CTGGACAGAA GTGGCCAAGG GAAGGTCTAT CCTCTTATCA ACCGAAGACG ATTTCAACAA 
ATGGACGTAC TTGAGGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA 
CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAATGATCC AGAAGTTGAG 
AAGAAGCAGG GATGGACAAC CGTAGGGGAT TTGGAAGGAT GTGTACATTA TAAAGTTGTA 
AAATATGAAA GAATCAAATT TCTGGTGATT GCTTTGAAGA GTTCTGTGGA AGTCTATGCG 
TGGGCACCAA AGCCATATCA CAAATTTATG GCCTTTAAGT CATTTGGAGA ATTGGTACAT 
AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA 
TCCTGTGCTG GATTCCATGC TGTTGATGTG GATTCAGGAT CAGTCTATGA CATTTATCTA 
CCAACACATG TAAGAAAGAA CCCACACTCT ATGATCCAGT GTAGCATCAA ACCCCATGCA 
ATCATCATCC TCCCCAATAC AGATGGAATG GAGCTTCTGG TGTGCTATGA AGATGAGGGG 
GTTTATGTAA ACACATATGG AAGGATCACC AAGGATGTAG TTCTACAGTG GGGAGAGATG 
CCTACATCAG TAGCATATAT TCGATCCAAT CAGACAATGG GCTGGGGAGA GAAGGCCATA 
GAGATCCGAT CTGTGGAAAC TGGTCACTTG GATGGTGTGT TCATGCACAA AAGGGCTCAA 
AGACTAAAAT TCTTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT 
GGCAGCAGTC AGGTTTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP4K4 ( XM_005264052.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264109.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu36829
Clone ID Related Accession (Same CDS sequence)	XM_005264052.3
Accession Version	XM_005264052.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264052.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC 
CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT 
GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC 
GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA 
GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC 
AGTATTGAGC CCAGGCTTCT GTGGGAGAGA GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT 
GGCAGCTCCT CAGGGTCCAG CAACTCAGGA TCCCAGCCCG GGTCTCACCC TGGGTCTCAG 
AGTGGCTCCG GGGAACGCTT CAGAGTGAGA TCATCATCCA AGTCTGAAGG CTCTCCATCT 
CAGCGCCTGG AAAATGCAGT GAAAAAACCT GAAGATAAAA AGGAAGTTTT CAGACCCCTC 
AAGCCTGCTG GCGAAGTGGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT 
GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG 
GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG 
GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC 
ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT 
CTAATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC 
TTTGCCGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT 
TCCTCCACCT CCTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA 
CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC 
AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
AGCGGAACAA CAGTGACATC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCAGAAGCC 
ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG 
CCACAGAGTG ACACCCCGGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAGATTCTG 
TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG 
GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTTATCAACC GAAGACGATT TCAACAAATG 
GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT 
GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG 
AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA 
TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCGTGG 
GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG 
CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC 
TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCAGGATCAG TCTATGACAT TTATCTACCA 
ACACATGTAA GAAAGAACCC ACACTCTATG ATCCAGTGTA GCATCAAACC CCATGCAATC 
ATCATCCTCC CCAATACAGA TGGAATGGAG CTTCTGGTGT GCTATGAAGA TGAGGGGGTT 
TATGTAAACA CATATGGAAG GATCACCAAG GATGTAGTTC TACAGTGGGG AGAGATGCCT 
ACATCAGTAG CATATATTCG ATCCAATCAG ACAATGGGCT GGGGAGAGAA GGCCATAGAG 
ATCCGATCTG TGGAAACTGG TCACTTGGAT GGTGTGTTCA TGCACAAAAG GGCTCAAAGA 
CTAAAATTCT TGTGTGAACG CAATGACAAG GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC 
AGCAGTCAGG TTTATTTCAT GACCTTAGGC AGGACTTCTC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264109.1
CDS	75..4088
Translation	MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEINMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRGLAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPSTEQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGESTLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREARRQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQAMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHYEPADRAREVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQSTVDQKRASHHESNGFAGRIHLLPDLLQQSHSSSTSSTSSSPSSSQPTPTMSPQTPQDKLTANETQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHVRKNPHSMIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW

Target ORF information:

RefSeq Version	XM_005264052.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264052.3

ORF Insert Sequence:

ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT 
AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG 
GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA 
AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA 
CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC 
AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA 
CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG 
CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG 
ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC 
GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC 
ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA 
GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG 
AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA 
GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC 
CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG 
TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA 
AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG 
CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG 
CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG 
CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA 
AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG 
TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA 
GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA 
GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG 
ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT 
CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA 
CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC 
GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT 
GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC 
CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT 
GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC 
GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA 
GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC 
AGTATTGAGC CCAGGCTTCT GTGGGAGAGA GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT 
GGCAGCTCCT CAGGGTCCAG CAACTCAGGA TCCCAGCCCG GGTCTCACCC TGGGTCTCAG 
AGTGGCTCCG GGGAACGCTT CAGAGTGAGA TCATCATCCA AGTCTGAAGG CTCTCCATCT 
CAGCGCCTGG AAAATGCAGT GAAAAAACCT GAAGATAAAA AGGAAGTTTT CAGACCCCTC 
AAGCCTGCTG GCGAAGTGGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT 
GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG 
GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG 
GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC 
ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT 
CTAATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC 
TTTGCCGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT 
TCCTCCACCT CCTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA 
CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC 
AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
AGCGGAACAA CAGTGACATC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCAGAAGCC 
ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG 
CCACAGAGTG ACACCCCGGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAGATTCTG 
TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG 
GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTTATCAACC GAAGACGATT TCAACAAATG 
GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT 
GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG 
AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA 
TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCGTGG 
GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG 
CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC 
TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCAGGATCAG TCTATGACAT TTATCTACCA 
ACACATGTAA GAAAGAACCC ACACTCTATG ATCCAGTGTA GCATCAAACC CCATGCAATC 
ATCATCCTCC CCAATACAGA TGGAATGGAG CTTCTGGTGT GCTATGAAGA TGAGGGGGTT 
TATGTAAACA CATATGGAAG GATCACCAAG GATGTAGTTC TACAGTGGGG AGAGATGCCT 
ACATCAGTAG CATATATTCG ATCCAATCAG ACAATGGGCT GGGGAGAGAA GGCCATAGAG 
ATCCGATCTG TGGAAACTGG TCACTTGGAT GGTGTGTTCA TGCACAAAAG GGCTCAAAGA 
CTAAAATTCT TGTGTGAACG CAATGACAAG GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC 
AGCAGTCAGG TTTATTTCAT GACCTTAGGC AGGACTTCTC TTCTGAGCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RBM4-SRSF3-MAP4K4 splicing cascade modulates the metastatic signature of colorectal cancer cell.
Biochimica et biophysica acta1865(2)259-272(2018 Feb)
Lin JC,Lee YC,Tan TH,Liang YC,Chuang HC,Fann YC,Johnson KR,Lin YJ

The Ser/Thr kinase MAP4K4 drives c-Met-induced motility and invasiveness in a cell-based model of SHH medulloblastoma.
SpringerPlus419(2015)
Santhana Kumar K,Tripolitsioti D,Ma M,Gr?hlert J,Egli KB,Fiaschetti G,Shalaby T,Grotzer MA,Baumgartner M

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MAP4K4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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