MAP4K4 cDNA ORF clone, Homo sapiens(human)

The following MAP4K4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP4K4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu92772 XM_017005349.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu92773 XM_017005350.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92774 XM_017005351.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92775 XM_017005352.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92776 XM_017005353.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92777 XM_017005354.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92778 XM_017005355.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92779 XM_017005356.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92780 XM_017005357.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92781 XM_017005358.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92782 XM_017005359.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92783 XM_017005360.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92784 XM_017005361.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92785 XM_017005362.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92786 XM_017005363.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92787 XM_017005364.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92788 XM_017005365.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92789 XM_017005366.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92790 XM_017005367.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92791 XM_017005368.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X34, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92792 XM_017005369.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X36, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92793 XM_017005370.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X38, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92794 XM_017005371.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X39, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92795 XM_017005372.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X41, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu92796 XM_017005373.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X42, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu92797 XM_017005374.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X43, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu92798 XM_017005375.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X44, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu20375 NM_145687.4
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $853.30
$1219.00
OHu20734 NM_145686.3
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $923.30
$1319.00
OHu21057 NM_004834.5
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $825.30
$1179.00
OHu21579 NM_001242560.2
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu22392 NM_001242559.2
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36834 XM_005264057.3
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36835 XM_005264058.3
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36836 XM_006712865.2
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36838 XM_005264059.3
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36839 XM_005264060.3
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36841 XM_005264062.3
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36842 XM_005264063.3
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36843 XM_005264064.3
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36844 XM_005264065.3
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36845 XM_005264066.3
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36846 XM_005264068.3
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X33, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36847 XM_005264069.2
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X35, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36848 XM_005264071.2
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X40, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36850 XM_006712868.2
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X45, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36851 XM_006712869.2
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X37, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36825 XM_005264048.2
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu36828 XM_005264050.3
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu36829 XM_005264052.3
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OHu20375 NM_145687.3 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $853.30
$1219.00
OHu21057 NM_004834.4 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $825.30
$1179.00
OHu21579 NM_001242560.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu22392 NM_001242559.1 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu92772
    Clone ID Related Accession (Same CDS sequence) XM_017005349.1
    Accession Version XM_017005349.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC
    AAGAACAATG TTTCCCCTGT CTCGCGATCC CATTCCTTCA GTGACCCTTC TCCCAAATTT
    GCACACCACC ATCTTCGTTC TCAGGACCCA TGTCCACCTT CCCGCAGTGA GGTGCTCAGT
    CAGAGCTCTG ACTCTAAGTC AGAGGCGCCT GACCCTACCC AAAAGGCTTG GTCTAGATCA
    GACAGTGACG AGGTGCCTCC AAGGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA
    CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT
    GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC
    TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC
    AGACCCCTCA AGCCTGCTGG CGAAGTGGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG
    GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA
    GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT
    GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG
    GAGGGCACTC TAATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCCGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC CTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT
    TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC
    AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT
    GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG
    ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT
    CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA
    GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA
    GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG
    GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT
    TATCTACCAA CACATATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT
    ACAGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT
    GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACATC AGTAGCATAT
    ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860838.1
    CDS75..4133
    Translation

    Target ORF information:

    RefSeq Version XM_017005349.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005349.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC
    AAGAACAATG TTTCCCCTGT CTCGCGATCC CATTCCTTCA GTGACCCTTC TCCCAAATTT
    GCACACCACC ATCTTCGTTC TCAGGACCCA TGTCCACCTT CCCGCAGTGA GGTGCTCAGT
    CAGAGCTCTG ACTCTAAGTC AGAGGCGCCT GACCCTACCC AAAAGGCTTG GTCTAGATCA
    GACAGTGACG AGGTGCCTCC AAGGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA
    CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT
    GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC
    TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC
    AGACCCCTCA AGCCTGCTGG CGAAGTGGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG
    GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA
    GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT
    GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG
    GAGGGCACTC TAATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCCGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC CTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT
    TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC
    AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT
    GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG
    ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT
    CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA
    GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA
    GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG
    GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT
    TATCTACCAA CACATATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT
    ACAGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT
    GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACATC AGTAGCATAT
    ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92773
    Clone ID Related Accession (Same CDS sequence) XM_017005350.1
    Accession Version XM_017005350.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT
    GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC
    CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT
    GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC
    GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA
    GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC
    AGCAGTATTG AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC
    AGTGGCAGCT CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT
    CAGAGTGGCT CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA
    TCTCAGCGCC TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC
    CTCAAGCCTG CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA
    GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG
    ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA
    GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA
    ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC
    ACTCTAATCG TCCGCCAGAG TACAGTTGAC CAAAAGCGTG CCAGCCATCA TGAGAGCAAT
    GGCTTTGCCG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC
    ACTTCCTCCA CCTCCTCCTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG
    ACACCCCAGG ACAAGCTCAC TGCTAATGAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA
    TCTAGCGGAA CAACAGTGAC ATCTGTGGTG GGATTTTCCT GTGATGGGAT GAGACCAGAA
    GCCATAAGGC AAGATCCTAC CCGGAAAGGC TCAGTGGTCA ATGTGAATCC TACCAACACT
    AGGCCACAGA GTGACACCCC GGAGATTCGT AAATACAAGA AGAGGTTTAA CTCTGAGATT
    CTGTGTGCTG CCTTATGGGG AGTGAATTTG CTAGTGGGTA CAGAGAGTGG CCTGATGCTG
    CTGGACAGAA GTGGCCAAGG GAAGGTCTAT CCTCTTATCA ACCGAAGACG ATTTCAACAA
    ATGGACGTAC TTGAGGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAATGATCC AGAAGTTGAG
    AAGAAGCAGG GATGGACAAC CGTAGGGGAT TTGGAAGGAT GTGTACATTA TAAAGTTGTA
    AAATATGAAA GAATCAAATT TCTGGTGATT GCTTTGAAGA GTTCTGTGGA AGTCTATGCG
    TGGGCACCAA AGCCATATCA CAAATTTATG GCCTTTAAGT CATTTGGAGA ATTGGTACAT
    AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GATTCAGGAT CAGTCTATGA CATTTATCTA
    CCAACACATA TCCAGTGTAG CATCAAACCC CATGCAATCA TCATCCTCCC CAATACAGAT
    GGAATGGAGC TTCTGGTGTG CTATGAAGAT GAGGGGGTTT ATGTAAACAC ATATGGAAGG
    ATCACCAAGG ATGTAGTTCT ACAGTGGGGA GAGATGCCTA CATCAGTAGC ATATATTCGA
    TCCAATCAGA CAATGGGCTG GGGAGAGAAG GCCATAGAGA TCCGATCTGT GGAAACTGGT
    CACTTGGATG GTGTGTTCAT GCACAAAAGG GCTCAAAGAC TAAAATTCTT GTGTGAACGC
    AATGACAAGG TGTTCTTTGC CTCTGTTCGG TCTGGTGGCA GCAGTCAGGT TTATTTCATG
    ACCTTAGGCA GGACTTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860839.1
    CDS75..4067
    Translation

    Target ORF information:

    RefSeq Version XM_017005350.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005350.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT
    GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC
    CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT
    GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC
    GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA
    GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC
    AGCAGTATTG AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC
    AGTGGCAGCT CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT
    CAGAGTGGCT CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA
    TCTCAGCGCC TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC
    CTCAAGCCTG CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA
    GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG
    ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA
    GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA
    ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC
    ACTCTAATCG TCCGCCAGAG TACAGTTGAC CAAAAGCGTG CCAGCCATCA TGAGAGCAAT
    GGCTTTGCCG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC
    ACTTCCTCCA CCTCCTCCTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG
    ACACCCCAGG ACAAGCTCAC TGCTAATGAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA
    TCTAGCGGAA CAACAGTGAC ATCTGTGGTG GGATTTTCCT GTGATGGGAT GAGACCAGAA
    GCCATAAGGC AAGATCCTAC CCGGAAAGGC TCAGTGGTCA ATGTGAATCC TACCAACACT
    AGGCCACAGA GTGACACCCC GGAGATTCGT AAATACAAGA AGAGGTTTAA CTCTGAGATT
    CTGTGTGCTG CCTTATGGGG AGTGAATTTG CTAGTGGGTA CAGAGAGTGG CCTGATGCTG
    CTGGACAGAA GTGGCCAAGG GAAGGTCTAT CCTCTTATCA ACCGAAGACG ATTTCAACAA
    ATGGACGTAC TTGAGGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAATGATCC AGAAGTTGAG
    AAGAAGCAGG GATGGACAAC CGTAGGGGAT TTGGAAGGAT GTGTACATTA TAAAGTTGTA
    AAATATGAAA GAATCAAATT TCTGGTGATT GCTTTGAAGA GTTCTGTGGA AGTCTATGCG
    TGGGCACCAA AGCCATATCA CAAATTTATG GCCTTTAAGT CATTTGGAGA ATTGGTACAT
    AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GATTCAGGAT CAGTCTATGA CATTTATCTA
    CCAACACATA TCCAGTGTAG CATCAAACCC CATGCAATCA TCATCCTCCC CAATACAGAT
    GGAATGGAGC TTCTGGTGTG CTATGAAGAT GAGGGGGTTT ATGTAAACAC ATATGGAAGG
    ATCACCAAGG ATGTAGTTCT ACAGTGGGGA GAGATGCCTA CATCAGTAGC ATATATTCGA
    TCCAATCAGA CAATGGGCTG GGGAGAGAAG GCCATAGAGA TCCGATCTGT GGAAACTGGT
    CACTTGGATG GTGTGTTCAT GCACAAAAGG GCTCAAAGAC TAAAATTCTT GTGTGAACGC
    AATGACAAGG TGTTCTTTGC CTCTGTTCGG TCTGGTGGCA GCAGTCAGGT TTATTTCATG
    ACCTTAGGCA GGACTTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92774
    Clone ID Related Accession (Same CDS sequence) XM_017005351.1
    Accession Version XM_017005351.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA
    CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG
    CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC
    TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC
    GGGGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG
    GAAAATGCAG TGAAAAAACC TGAAGATAAA AAGGAAGTTT TCAGACCCCT CAAGCCTGCT
    GATCTGACCG CACTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTACGGCC ACCTCACAAA
    GTAACGGACT ACTCCTCATC CAGTGAGGAG TCGGGGACGA CGGATGAGGA GGACGACGAT
    GTGGAGCAGG AAGGGGCTGA CGAGTCCACC TCAGGACCAG AGGACACCAG AGCAGCGTCA
    TCTCTGAATT TGAGCAATGG TGAAACGGAA TCTGTGAAAA CCATGATTGT CCATGATGAT
    GTAGAAAGTG AGCCGGCCAT GACCCCATCC AAGGAGGGCA CTCTAATCGT CCGCCAGAGT
    ACAGTTGACC AAAAGCGTGC CAGCCATCAT GAGAGCAATG GCTTTGCCGG TCGCATTCAC
    CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC
    CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTCACT
    GCTAATGAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGCGGAAC AACAGTGACA
    TCTGTGGTGG GATTTTCCTG TGATGGGATG AGACCAGAAG CCATAAGGCA AGATCCTACC
    CGGAAAGGCT CAGTGGTCAA TGTGAATCCT ACCAACACTA GGCCACAGAG TGACACCCCG
    GAGATTCGTA AATACAAGAA GAGGTTTAAC TCTGAGATTC TGTGTGCTGC CTTATGGGGA
    GTGAATTTGC TAGTGGGTAC AGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG
    AAGGTCTATC CTCTTATCAA CCGAAGACGA TTTCAACAAA TGGACGTACT TGAGGGCTTG
    AATGTCTTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG
    TTAAGAAATA AAATACTTCA CAATGATCCA GAAGTTGAGA AGAAGCAGGG ATGGACAACC
    GTAGGGGATT TGGAAGGATG TGTACATTAT AAAGTTGTAA AATATGAAAG AATCAAATTT
    CTGGTGATTG CTTTGAAGAG TTCTGTGGAA GTCTATGCGT GGGCACCAAA GCCATATCAC
    AAATTTATGG CCTTTAAGTC ATTTGGAGAA TTGGTACATA AGCCATTACT GGTGGATCTC
    ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT
    GTTGATGTGG ATTCAGGATC AGTCTATGAC ATTTATCTAC CAACACATGT AAGAAAGAAC
    CCACACTCTA TGATCCAGTG TAGCATCAAA CCCCATGCAA TCATCATCCT CCCCAATACA
    GATGGAATGG AGCTTCTGGT GTGCTATGAA GATGAGGGGG TTTATGTAAA CACATATGGA
    AGGATCACCA AGGATGTAGT TCTACAGTGG GGAGAGATGC CTACATCAGT AGCATATATT
    CGATCCAATC AGACAATGGG CTGGGGAGAG AAGGCCATAG AGATCCGATC TGTGGAAACT
    GGTCACTTGG ATGGTGTGTT CATGCACAAA AGGGCTCAAA GACTAAAATT CTTGTGTGAA
    CGCAATGACA AGGTGTTCTT TGCCTCTGTT CGGTCTGGTG GCAGCAGTCA GGTTTATTTC
    ATGACCTTAG GCAGGACTTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860840.1
    CDS75..4010
    Translation

    Target ORF information:

    RefSeq Version XM_017005351.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005351.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA
    CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG
    CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC
    TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC
    GGGGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG
    GAAAATGCAG TGAAAAAACC TGAAGATAAA AAGGAAGTTT TCAGACCCCT CAAGCCTGCT
    GATCTGACCG CACTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTACGGCC ACCTCACAAA
    GTAACGGACT ACTCCTCATC CAGTGAGGAG TCGGGGACGA CGGATGAGGA GGACGACGAT
    GTGGAGCAGG AAGGGGCTGA CGAGTCCACC TCAGGACCAG AGGACACCAG AGCAGCGTCA
    TCTCTGAATT TGAGCAATGG TGAAACGGAA TCTGTGAAAA CCATGATTGT CCATGATGAT
    GTAGAAAGTG AGCCGGCCAT GACCCCATCC AAGGAGGGCA CTCTAATCGT CCGCCAGAGT
    ACAGTTGACC AAAAGCGTGC CAGCCATCAT GAGAGCAATG GCTTTGCCGG TCGCATTCAC
    CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC
    CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTCACT
    GCTAATGAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGCGGAAC AACAGTGACA
    TCTGTGGTGG GATTTTCCTG TGATGGGATG AGACCAGAAG CCATAAGGCA AGATCCTACC
    CGGAAAGGCT CAGTGGTCAA TGTGAATCCT ACCAACACTA GGCCACAGAG TGACACCCCG
    GAGATTCGTA AATACAAGAA GAGGTTTAAC TCTGAGATTC TGTGTGCTGC CTTATGGGGA
    GTGAATTTGC TAGTGGGTAC AGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG
    AAGGTCTATC CTCTTATCAA CCGAAGACGA TTTCAACAAA TGGACGTACT TGAGGGCTTG
    AATGTCTTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG
    TTAAGAAATA AAATACTTCA CAATGATCCA GAAGTTGAGA AGAAGCAGGG ATGGACAACC
    GTAGGGGATT TGGAAGGATG TGTACATTAT AAAGTTGTAA AATATGAAAG AATCAAATTT
    CTGGTGATTG CTTTGAAGAG TTCTGTGGAA GTCTATGCGT GGGCACCAAA GCCATATCAC
    AAATTTATGG CCTTTAAGTC ATTTGGAGAA TTGGTACATA AGCCATTACT GGTGGATCTC
    ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT
    GTTGATGTGG ATTCAGGATC AGTCTATGAC ATTTATCTAC CAACACATGT AAGAAAGAAC
    CCACACTCTA TGATCCAGTG TAGCATCAAA CCCCATGCAA TCATCATCCT CCCCAATACA
    GATGGAATGG AGCTTCTGGT GTGCTATGAA GATGAGGGGG TTTATGTAAA CACATATGGA
    AGGATCACCA AGGATGTAGT TCTACAGTGG GGAGAGATGC CTACATCAGT AGCATATATT
    CGATCCAATC AGACAATGGG CTGGGGAGAG AAGGCCATAG AGATCCGATC TGTGGAAACT
    GGTCACTTGG ATGGTGTGTT CATGCACAAA AGGGCTCAAA GACTAAAATT CTTGTGTGAA
    CGCAATGACA AGGTGTTCTT TGCCTCTGTT CGGTCTGGTG GCAGCAGTCA GGTTTATTTC
    ATGACCTTAG GCAGGACTTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92775
    Clone ID Related Accession (Same CDS sequence) XM_017005352.1
    Accession Version XM_017005352.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA
    CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT
    GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC
    TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC
    TCCGGGGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC
    CTGGAAAATG CAGTGAAAAA ACCTGAAGAT AAAAAGGAAG TTTTCAGACC CCTCAAGCCT
    GCTGGCGAAG TGGATCTGAC CGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTACGG
    CCACCTCACA AAGTAACGGA CTACTCCTCA TCCAGTGAGG AGTCGGGGAC GACGGATGAG
    GAGGACGACG ATGTGGAGCA GGAAGGGGCT GACGAGTCCA CCTCAGGACC AGAGGACACC
    AGAGCAGCGT CATCTCTGAA TTTGAGCAAT GGTGAAACGG AATCTGTGAA AACCATGATT
    GTCCATGATG ATGTAGAAAG TGAGCCGGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC
    GTCCGCCAGA GTACAGTTGA CCAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCC
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC
    ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG
    GACAAGCTCA CTGCTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGCGGA
    ACAACAGTGA CATCTGTGGT GGGATTTTCC TGTGATGGGA TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CCCGGAAAGG CTCAGTGGTC AATGTGAATC CTACCAACAC TAGGCCACAG
    AGTGACACCC CGGAGATTCG TAAATACAAG AAGAGGTTTA ACTCTGAGAT TCTGTGTGCT
    GCCTTATGGG GAGTGAATTT GCTAGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA
    AGTGGCCAAG GGAAGGTCTA TCCTCTTATC AACCGAAGAC GATTTCAACA AATGGACGTA
    CTTGAGGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC
    TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAATGATC CAGAAGTTGA GAAGAAGCAG
    GGATGGACAA CCGTAGGGGA TTTGGAAGGA TGTGTACATT ATAAAGTTGT AAAATATGAA
    AGAATCAAAT TTCTGGTGAT TGCTTTGAAG AGTTCTGTGG AAGTCTATGC GTGGGCACCA
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AATTGGTACA TAAGCCATTA
    CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT
    GGATTCCATG CTGTTGATGT GGATTCAGGA TCAGTCTATG ACATTTATCT ACCAACACAT
    ATCCAGTGTA GCATCAAACC CCATGCAATC ATCATCCTCC CCAATACAGA TGGAATGGAG
    CTTCTGGTGT GCTATGAAGA TGAGGGGGTT TATGTAAACA CATATGGAAG GATCACCAAG
    GATGTAGTTC TACAGTGGGG AGAGATGCCT ACATCAGTAG CATATATTCG ATCCAATCAG
    ACAATGGGCT GGGGAGAGAA GGCCATAGAG ATCCGATCTG TGGAAACTGG TCACTTGGAT
    GGTGTGTTCA TGCACAAAAG GGCTCAAAGA CTAAAATTCT TGTGTGAACG CAATGACAAG
    GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC AGCAGTCAGG TTTATTTCAT GACCTTAGGC
    AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860841.1
    CDS75..3998
    Translation

    Target ORF information:

    RefSeq Version XM_017005352.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005352.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA
    CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT
    GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC
    TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC
    TCCGGGGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC
    CTGGAAAATG CAGTGAAAAA ACCTGAAGAT AAAAAGGAAG TTTTCAGACC CCTCAAGCCT
    GCTGGCGAAG TGGATCTGAC CGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTACGG
    CCACCTCACA AAGTAACGGA CTACTCCTCA TCCAGTGAGG AGTCGGGGAC GACGGATGAG
    GAGGACGACG ATGTGGAGCA GGAAGGGGCT GACGAGTCCA CCTCAGGACC AGAGGACACC
    AGAGCAGCGT CATCTCTGAA TTTGAGCAAT GGTGAAACGG AATCTGTGAA AACCATGATT
    GTCCATGATG ATGTAGAAAG TGAGCCGGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC
    GTCCGCCAGA GTACAGTTGA CCAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCC
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC
    ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG
    GACAAGCTCA CTGCTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGCGGA
    ACAACAGTGA CATCTGTGGT GGGATTTTCC TGTGATGGGA TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CCCGGAAAGG CTCAGTGGTC AATGTGAATC CTACCAACAC TAGGCCACAG
    AGTGACACCC CGGAGATTCG TAAATACAAG AAGAGGTTTA ACTCTGAGAT TCTGTGTGCT
    GCCTTATGGG GAGTGAATTT GCTAGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA
    AGTGGCCAAG GGAAGGTCTA TCCTCTTATC AACCGAAGAC GATTTCAACA AATGGACGTA
    CTTGAGGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC
    TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAATGATC CAGAAGTTGA GAAGAAGCAG
    GGATGGACAA CCGTAGGGGA TTTGGAAGGA TGTGTACATT ATAAAGTTGT AAAATATGAA
    AGAATCAAAT TTCTGGTGAT TGCTTTGAAG AGTTCTGTGG AAGTCTATGC GTGGGCACCA
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AATTGGTACA TAAGCCATTA
    CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT
    GGATTCCATG CTGTTGATGT GGATTCAGGA TCAGTCTATG ACATTTATCT ACCAACACAT
    ATCCAGTGTA GCATCAAACC CCATGCAATC ATCATCCTCC CCAATACAGA TGGAATGGAG
    CTTCTGGTGT GCTATGAAGA TGAGGGGGTT TATGTAAACA CATATGGAAG GATCACCAAG
    GATGTAGTTC TACAGTGGGG AGAGATGCCT ACATCAGTAG CATATATTCG ATCCAATCAG
    ACAATGGGCT GGGGAGAGAA GGCCATAGAG ATCCGATCTG TGGAAACTGG TCACTTGGAT
    GGTGTGTTCA TGCACAAAAG GGCTCAAAGA CTAAAATTCT TGTGTGAACG CAATGACAAG
    GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC AGCAGTCAGG TTTATTTCAT GACCTTAGGC
    AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92776
    Clone ID Related Accession (Same CDS sequence) XM_017005353.1
    Accession Version XM_017005353.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3921bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA
    CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG
    CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC
    TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC
    GGGGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG
    GAAAATGCAG TGAAAAAACC TGAAGATAAA AAGGAAGTTT TCAGACCCCT CAAGCCTGCT
    GGCGAAGTGG ATCTGACCGC ACTGGCCAAA GAGCTTCGAG CAGTGGAAGA TGTACGGCCA
    CCTCACAAAG TAACGGACTA CTCCTCATCC AGTGAGGAGT CGGGGACGAC GGATGAGGAG
    GACGACGATG TGGAGCAGGA AGGGGCTGAC GAGTCCACCT CAGGACCAGA GGACACCAGA
    GCAGCGTCAT CTCTGAATTT GAGCAATGGT GAAACGGAAT CTGTGAAAAC CATGATTGTC
    CATGATGATG TAGAAAGTGA GCCGGCCATG ACCCCATCCA AGGAGGGCAC TCTAATCGTC
    CGCCAGAGTA CAGTTGACCA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCCTCCTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTCACTG CTAATGAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA
    ACAGTGACAT CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA
    GATCCTACCC GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT
    GACACCCCGG AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC
    TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT
    GGCCAAGGGA AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT
    GAGGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT
    TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA
    TGGACAACCG TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA
    ATCAAATTTC TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG
    CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG
    GTGGATCTCA CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA
    TTCCATGCTG TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATATC
    CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT
    CTGGTGTGCT ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT
    GTAGTTCTAC AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA
    ATGGGCTGGG GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT
    GTGTTCATGC ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG
    TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860842.1
    CDS75..3995
    Translation

    Target ORF information:

    RefSeq Version XM_017005353.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005353.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA
    CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG
    CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC
    TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC
    GGGGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG
    GAAAATGCAG TGAAAAAACC TGAAGATAAA AAGGAAGTTT TCAGACCCCT CAAGCCTGCT
    GGCGAAGTGG ATCTGACCGC ACTGGCCAAA GAGCTTCGAG CAGTGGAAGA TGTACGGCCA
    CCTCACAAAG TAACGGACTA CTCCTCATCC AGTGAGGAGT CGGGGACGAC GGATGAGGAG
    GACGACGATG TGGAGCAGGA AGGGGCTGAC GAGTCCACCT CAGGACCAGA GGACACCAGA
    GCAGCGTCAT CTCTGAATTT GAGCAATGGT GAAACGGAAT CTGTGAAAAC CATGATTGTC
    CATGATGATG TAGAAAGTGA GCCGGCCATG ACCCCATCCA AGGAGGGCAC TCTAATCGTC
    CGCCAGAGTA CAGTTGACCA AAAGCGTGCC AGCCATCATG AGAGCAATGG CTTTGCCGGT
    CGCATTCACC TCTTGCCAGA TCTCTTACAG CAAAGCCATT CCTCCTCCAC TTCCTCCACC
    TCCTCCTCCC CATCCTCCAG CCAGCCGACA CCCACCATGT CCCCACAGAC ACCCCAGGAC
    AAGCTCACTG CTAATGAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA
    ACAGTGACAT CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA
    GATCCTACCC GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT
    GACACCCCGG AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC
    TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT
    GGCCAAGGGA AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT
    GAGGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT
    TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA
    TGGACAACCG TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA
    ATCAAATTTC TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG
    CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG
    GTGGATCTCA CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA
    TTCCATGCTG TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATATC
    CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT
    CTGGTGTGCT ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT
    GTAGTTCTAC AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA
    ATGGGCTGGG GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT
    GTGTTCATGC ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG
    TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92777
    Clone ID Related Accession (Same CDS sequence) XM_017005354.1
    Accession Version XM_017005354.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC
    CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA
    TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT
    AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA
    GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA
    TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA
    TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT
    GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA
    CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC
    TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA
    GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG
    AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG
    CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGAGTAC AGTTGACCAA
    AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCCGGTC GCATTCACCT CTTGCCAGAT
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCCTCCCC ATCCTCCAGC
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA
    TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA
    GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA
    TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA
    GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT
    CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA
    ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG
    GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT
    TCAGGATCAG TCTATGACAT TTATCTACCA ACACATATCC AGTGTAGCAT CAAACCCCAT
    GCAATCATCA TCCTCCCCAA TACAGATGGA ATGGAGCTTC TGGTGTGCTA TGAAGATGAG
    GGGGTTTATG TAAACACATA TGGAAGGATC ACCAAGGATG TAGTTCTACA GTGGGGAGAG
    ATGCCTACAT CAGTAGCATA TATTCGATCC AATCAGACAA TGGGCTGGGG AGAGAAGGCC
    ATAGAGATCC GATCTGTGGA AACTGGTCAC TTGGATGGTG TGTTCATGCA CAAAAGGGCT
    CAAAGACTAA AATTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC TGTTCGGTCT
    GGTGGCAGCA GTCAGGTTTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860843.1
    CDS75..3974
    Translation

    Target ORF information:

    RefSeq Version XM_017005354.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005354.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC
    CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA
    TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT
    AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA
    GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA
    TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA
    TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT
    GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA
    CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC
    TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA
    GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG
    AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG
    CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGAGTAC AGTTGACCAA
    AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCCGGTC GCATTCACCT CTTGCCAGAT
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCCTCCCC ATCCTCCAGC
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA
    TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA
    GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA
    TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA
    GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT
    CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA
    ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG
    GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT
    TCAGGATCAG TCTATGACAT TTATCTACCA ACACATATCC AGTGTAGCAT CAAACCCCAT
    GCAATCATCA TCCTCCCCAA TACAGATGGA ATGGAGCTTC TGGTGTGCTA TGAAGATGAG
    GGGGTTTATG TAAACACATA TGGAAGGATC ACCAAGGATG TAGTTCTACA GTGGGGAGAG
    ATGCCTACAT CAGTAGCATA TATTCGATCC AATCAGACAA TGGGCTGGGG AGAGAAGGCC
    ATAGAGATCC GATCTGTGGA AACTGGTCAC TTGGATGGTG TGTTCATGCA CAAAAGGGCT
    CAAAGACTAA AATTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC TGTTCGGTCT
    GGTGGCAGCA GTCAGGTTTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92778
    Clone ID Related Accession (Same CDS sequence) XM_017005355.1
    Accession Version XM_017005355.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC
    CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA
    TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT
    AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA
    GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA
    TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA
    TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT
    GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG ATCTGACCGC ACTGGCCAAA
    GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC
    AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC
    GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT
    GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG
    ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGAGTA CAGTTGACCA AAAGCGTGCC
    AGCCATCATG AGAGCAATGG CTTTGCCGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG
    CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCCTCCC CATCCTCCAG CCAGCCGACA
    CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTG CTAATGAGAC TCAGTCCGCT
    AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA
    TTACTACAGA TTTCTCCATC TAGCGGAACA ACAGTGACAT CTGTGGTGGG ATTTTCCTGT
    GATGGGATGA GACCAGAAGC CATAAGGCAA GATCCTACCC GGAAAGGCTC AGTGGTCAAT
    GTGAATCCTA CCAACACTAG GCCACAGAGT GACACCCCGG AGATTCGTAA ATACAAGAAG
    AGGTTTAACT CTGAGATTCT GTGTGCTGCC TTATGGGGAG TGAATTTGCT AGTGGGTACA
    GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTCTATCC TCTTATCAAC
    CGAAGACGAT TTCAACAAAT GGACGTACTT GAGGGCTTGA ATGTCTTGGT GACAATATCT
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC
    AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACAACCG TAGGGGATTT GGAAGGATGT
    GTACATTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC TGGTGATTGC TTTGAAGAGT
    TCTGTGGAAG TCTATGCGTG GGCACCAAAG CCATATCACA AATTTATGGC CTTTAAGTCA
    TTTGGAGAAT TGGTACATAA GCCATTACTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG
    TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA TTCAGGATCA
    GTCTATGACA TTTATCTACC AACACATATC CAGTGTAGCA TCAAACCCCA TGCAATCATC
    ATCCTCCCCA ATACAGATGG AATGGAGCTT CTGGTGTGCT ATGAAGATGA GGGGGTTTAT
    GTAAACACAT ATGGAAGGAT CACCAAGGAT GTAGTTCTAC AGTGGGGAGA GATGCCTACA
    TCAGTAGCAT ATATTCGATC CAATCAGACA ATGGGCTGGG GAGAGAAGGC CATAGAGATC
    CGATCTGTGG AAACTGGTCA CTTGGATGGT GTGTTCATGC ACAAAAGGGC TCAAAGACTA
    AAATTCTTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC
    AGTCAGGTTT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860844.1
    CDS75..3965
    Translation

    Target ORF information:

    RefSeq Version XM_017005355.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005355.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC
    CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA
    TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT
    AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA
    GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA
    TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA
    TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT
    GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG ATCTGACCGC ACTGGCCAAA
    GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC
    AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC
    GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT
    GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG
    ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGAGTA CAGTTGACCA AAAGCGTGCC
    AGCCATCATG AGAGCAATGG CTTTGCCGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG
    CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCCTCCC CATCCTCCAG CCAGCCGACA
    CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTG CTAATGAGAC TCAGTCCGCT
    AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA
    TTACTACAGA TTTCTCCATC TAGCGGAACA ACAGTGACAT CTGTGGTGGG ATTTTCCTGT
    GATGGGATGA GACCAGAAGC CATAAGGCAA GATCCTACCC GGAAAGGCTC AGTGGTCAAT
    GTGAATCCTA CCAACACTAG GCCACAGAGT GACACCCCGG AGATTCGTAA ATACAAGAAG
    AGGTTTAACT CTGAGATTCT GTGTGCTGCC TTATGGGGAG TGAATTTGCT AGTGGGTACA
    GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTCTATCC TCTTATCAAC
    CGAAGACGAT TTCAACAAAT GGACGTACTT GAGGGCTTGA ATGTCTTGGT GACAATATCT
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC
    AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACAACCG TAGGGGATTT GGAAGGATGT
    GTACATTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC TGGTGATTGC TTTGAAGAGT
    TCTGTGGAAG TCTATGCGTG GGCACCAAAG CCATATCACA AATTTATGGC CTTTAAGTCA
    TTTGGAGAAT TGGTACATAA GCCATTACTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG
    TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA TTCAGGATCA
    GTCTATGACA TTTATCTACC AACACATATC CAGTGTAGCA TCAAACCCCA TGCAATCATC
    ATCCTCCCCA ATACAGATGG AATGGAGCTT CTGGTGTGCT ATGAAGATGA GGGGGTTTAT
    GTAAACACAT ATGGAAGGAT CACCAAGGAT GTAGTTCTAC AGTGGGGAGA GATGCCTACA
    TCAGTAGCAT ATATTCGATC CAATCAGACA ATGGGCTGGG GAGAGAAGGC CATAGAGATC
    CGATCTGTGG AAACTGGTCA CTTGGATGGT GTGTTCATGC ACAAAAGGGC TCAAAGACTA
    AAATTCTTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC
    AGTCAGGTTT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92779
    Clone ID Related Accession (Same CDS sequence) XM_017005356.1
    Accession Version XM_017005356.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC
    GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC
    AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA
    AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GATCTGACCG CACTGGCCAA AGAGCTTCGA
    GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG
    TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC
    TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA
    TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC
    AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT
    GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT
    TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG
    TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG
    ATTTCTCCAT CTAGCGGAAC AACAGTGACA TCTGTGGTGG GATTTTCCTG TGATGGGATG
    AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT
    ACCAACACTA GGCCACAGAG TGACACCCCG GAGATTCGTA AATACAAGAA GAGGTTTAAC
    TCTGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC
    CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTTATCAA CCGAAGACGA
    TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG
    GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA
    GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT
    AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAGAG TTCTGTGGAA
    GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA
    TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCAGGATC AGTCTATGAC
    ATTTATCTAC CAACACATGT AAGAAAGAAC CCACACTCTA TGATCCAGTG TAGCATCAAA
    CCCCATGCAA TCATCATCCT CCCCAATACA GATGGAATGG AGCTTCTGGT GTGCTATGAA
    GATGAGGGGG TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTAGT TCTACAGTGG
    GGAGAGATGC CTACATCAGT AGCATATATT CGATCCAATC AGACAATGGG CTGGGGAGAG
    AAGGCCATAG AGATCCGATC TGTGGAAACT GGTCACTTGG ATGGTGTGTT CATGCACAAA
    AGGGCTCAAA GACTAAAATT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCTGTT
    CGGTCTGGTG GCAGCAGTCA GGTTTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860845.1
    CDS75..3920
    Translation

    Target ORF information:

    RefSeq Version XM_017005356.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005356.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC
    GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC
    AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA
    AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GATCTGACCG CACTGGCCAA AGAGCTTCGA
    GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG
    TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC
    TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA
    TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC
    AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT
    GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT
    TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG
    TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG
    ATTTCTCCAT CTAGCGGAAC AACAGTGACA TCTGTGGTGG GATTTTCCTG TGATGGGATG
    AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT
    ACCAACACTA GGCCACAGAG TGACACCCCG GAGATTCGTA AATACAAGAA GAGGTTTAAC
    TCTGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC
    CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTTATCAA CCGAAGACGA
    TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG
    GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA
    GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT
    AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAGAG TTCTGTGGAA
    GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA
    TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCAGGATC AGTCTATGAC
    ATTTATCTAC CAACACATGT AAGAAAGAAC CCACACTCTA TGATCCAGTG TAGCATCAAA
    CCCCATGCAA TCATCATCCT CCCCAATACA GATGGAATGG AGCTTCTGGT GTGCTATGAA
    GATGAGGGGG TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTAGT TCTACAGTGG
    GGAGAGATGC CTACATCAGT AGCATATATT CGATCCAATC AGACAATGGG CTGGGGAGAG
    AAGGCCATAG AGATCCGATC TGTGGAAACT GGTCACTTGG ATGGTGTGTT CATGCACAAA
    AGGGCTCAAA GACTAAAATT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCTGTT
    CGGTCTGGTG GCAGCAGTCA GGTTTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92780
    Clone ID Related Accession (Same CDS sequence) XM_017005357.1
    Accession Version XM_017005357.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC
    GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC
    AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA
    AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA
    GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC
    AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC
    GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT
    GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG
    ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGAGTA CAGTTGACCA AAAGCGTGCC
    AGCCATCATG AGAGCAATGG CTTTGCCGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG
    CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCCTCCC CATCCTCCAG CCAGCCGACA
    CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTG CTAATGAGAC TCAGTCCGCT
    AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA
    TTACTACAGA TTTCTCCATC TAGCGGAACA ACAGTGACAT CTGTGGTGGG ATTTTCCTGT
    GATGGGATGA GACCAGAAGC CATAAGGCAA GATCCTACCC GGAAAGGCTC AGTGGTCAAT
    GTGAATCCTA CCAACACTAG GCCACAGAGT GACACCCCGG AGATTCGTAA ATACAAGAAG
    AGGTTTAACT CTGAGATTCT GTGTGCTGCC TTATGGGGAG TGAATTTGCT AGTGGGTACA
    GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTCTATCC TCTTATCAAC
    CGAAGACGAT TTCAACAAAT GGACGTACTT GAGGGCTTGA ATGTCTTGGT GACAATATCT
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC
    AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACAACCG TAGGGGATTT GGAAGGATGT
    GTACATTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC TGGTGATTGC TTTGAAGAGT
    TCTGTGGAAG TCTATGCGTG GGCACCAAAG CCATATCACA AATTTATGGC CTTTAAGTCA
    TTTGGAGAAT TGGTACATAA GCCATTACTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG
    TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA TTCAGGATCA
    GTCTATGACA TTTATCTACC AACACATATC CAGTGTAGCA TCAAACCCCA TGCAATCATC
    ATCCTCCCCA ATACAGATGG AATGGAGCTT CTGGTGTGCT ATGAAGATGA GGGGGTTTAT
    GTAAACACAT ATGGAAGGAT CACCAAGGAT GTAGTTCTAC AGTGGGGAGA GATGCCTACA
    TCAGTAGCAT ATATTCGATC CAATCAGACA ATGGGCTGGG GAGAGAAGGC CATAGAGATC
    CGATCTGTGG AAACTGGTCA CTTGGATGGT GTGTTCATGC ACAAAAGGGC TCAAAGACTA
    AAATTCTTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC
    AGTCAGGTTT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860846.1
    CDS75..3905
    Translation

    Target ORF information:

    RefSeq Version XM_017005357.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005357.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC
    GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC
    AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA
    AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA
    GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC
    AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC
    GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT
    GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG
    ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGAGTA CAGTTGACCA AAAGCGTGCC
    AGCCATCATG AGAGCAATGG CTTTGCCGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG
    CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCCTCCC CATCCTCCAG CCAGCCGACA
    CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTG CTAATGAGAC TCAGTCCGCT
    AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA
    TTACTACAGA TTTCTCCATC TAGCGGAACA ACAGTGACAT CTGTGGTGGG ATTTTCCTGT
    GATGGGATGA GACCAGAAGC CATAAGGCAA GATCCTACCC GGAAAGGCTC AGTGGTCAAT
    GTGAATCCTA CCAACACTAG GCCACAGAGT GACACCCCGG AGATTCGTAA ATACAAGAAG
    AGGTTTAACT CTGAGATTCT GTGTGCTGCC TTATGGGGAG TGAATTTGCT AGTGGGTACA
    GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTCTATCC TCTTATCAAC
    CGAAGACGAT TTCAACAAAT GGACGTACTT GAGGGCTTGA ATGTCTTGGT GACAATATCT
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC
    AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACAACCG TAGGGGATTT GGAAGGATGT
    GTACATTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC TGGTGATTGC TTTGAAGAGT
    TCTGTGGAAG TCTATGCGTG GGCACCAAAG CCATATCACA AATTTATGGC CTTTAAGTCA
    TTTGGAGAAT TGGTACATAA GCCATTACTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG
    TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA TTCAGGATCA
    GTCTATGACA TTTATCTACC AACACATATC CAGTGTAGCA TCAAACCCCA TGCAATCATC
    ATCCTCCCCA ATACAGATGG AATGGAGCTT CTGGTGTGCT ATGAAGATGA GGGGGTTTAT
    GTAAACACAT ATGGAAGGAT CACCAAGGAT GTAGTTCTAC AGTGGGGAGA GATGCCTACA
    TCAGTAGCAT ATATTCGATC CAATCAGACA ATGGGCTGGG GAGAGAAGGC CATAGAGATC
    CGATCTGTGG AAACTGGTCA CTTGGATGGT GTGTTCATGC ACAAAAGGGC TCAAAGACTA
    AAATTCTTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC
    AGTCAGGTTT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92781
    Clone ID Related Accession (Same CDS sequence) XM_017005358.1
    Accession Version XM_017005358.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG TATTGAGCCC AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG
    GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA GGGTCCAGCA ACTCAGGATC CCAGCCCGGG
    TCTCACCCTG GGTCTCAGAG TGGCTCCGGG GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGCT CTCCATCTCA GCGCCTGGAA AATGCAGTGA AAAAACCTGA AGATAAAAAG
    GAAGTTTTCA GACCCCTCAA GCCTGCTGGC GAAGTGGATC TGACCGCACT GGCCAAAGAG
    CTTCGAGCAG TGGAAGATGT ACGGCCACCT CACAAAGTAA CGGACTACTC CTCATCCAGT
    GAGGAGTCGG GGACGACGGA TGAGGAGGAC GACGATGTGG AGCAGGAAGG GGCTGACGAG
    TCCACCTCAG GACCAGAGGA CACCAGAGCA GCGTCATCTC TGAATTTGAG CAATGGTGAA
    ACGGAATCTG TGAAAACCAT GATTGTCCAT GATGATGTAG AAAGTGAGCC GGCCATGACC
    CCATCCAAGG AGGGCACTCT AATCGTCCGC CAGAGTACAG TTGACCAAAA GCGTGCCAGC
    CATCATGAGA GCAATGGCTT TGCCGGTCGC ATTCACCTCT TGCCAGATCT CTTACAGCAA
    AGCCATTCCT CCTCCACTTC CTCCACCTCC TCCTCCCCAT CCTCCAGCCA GCCGACACCC
    ACCATGTCCC CACAGACACC CCAGGACAAG CTCACTGCTA ATGAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA
    CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT
    GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG
    AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG
    TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA
    AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT
    GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA
    CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC
    TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA AACCCCATGC AATCATCATC
    CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA
    AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA
    GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA
    TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA
    TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT
    CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860847.1
    CDS75..3902
    Translation

    Target ORF information:

    RefSeq Version XM_017005358.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005358.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG TATTGAGCCC AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG
    GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA GGGTCCAGCA ACTCAGGATC CCAGCCCGGG
    TCTCACCCTG GGTCTCAGAG TGGCTCCGGG GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGCT CTCCATCTCA GCGCCTGGAA AATGCAGTGA AAAAACCTGA AGATAAAAAG
    GAAGTTTTCA GACCCCTCAA GCCTGCTGGC GAAGTGGATC TGACCGCACT GGCCAAAGAG
    CTTCGAGCAG TGGAAGATGT ACGGCCACCT CACAAAGTAA CGGACTACTC CTCATCCAGT
    GAGGAGTCGG GGACGACGGA TGAGGAGGAC GACGATGTGG AGCAGGAAGG GGCTGACGAG
    TCCACCTCAG GACCAGAGGA CACCAGAGCA GCGTCATCTC TGAATTTGAG CAATGGTGAA
    ACGGAATCTG TGAAAACCAT GATTGTCCAT GATGATGTAG AAAGTGAGCC GGCCATGACC
    CCATCCAAGG AGGGCACTCT AATCGTCCGC CAGAGTACAG TTGACCAAAA GCGTGCCAGC
    CATCATGAGA GCAATGGCTT TGCCGGTCGC ATTCACCTCT TGCCAGATCT CTTACAGCAA
    AGCCATTCCT CCTCCACTTC CTCCACCTCC TCCTCCCCAT CCTCCAGCCA GCCGACACCC
    ACCATGTCCC CACAGACACC CCAGGACAAG CTCACTGCTA ATGAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA
    CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT
    GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG
    AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG
    TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA
    AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT
    GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA
    CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC
    TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA AACCCCATGC AATCATCATC
    CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA
    AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA
    GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA
    TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA
    TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT
    CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92782
    Clone ID Related Accession (Same CDS sequence) XM_017005359.1
    Accession Version XM_017005359.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT
    GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC
    CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT
    GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC
    GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA
    GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC
    AGCAGTATTG AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC
    AGTGGCAGCT CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT
    CAGAGTGGCT CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA
    TCTCAGCGCC TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC
    CTCAAGCCTG CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA
    GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG
    ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA
    GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA
    ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC
    ACTCTAATCG TCCGCCAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA
    ACAGTGACAT CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA
    GATCCTACCC GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT
    GACACCCCGG AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC
    TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT
    GGCCAAGGGA AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT
    GAGGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT
    TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA
    TGGACAACCG TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA
    ATCAAATTTC TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG
    CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG
    GTGGATCTCA CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA
    TTCCATGCTG TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATGTA
    AGAAAGAACC CACACTCTAT GATCCAGTGT AGCATCAAAC CCCATGCAAT CATCATCCTC
    CCCAATACAG ATGGAATGGA GCTTCTGGTG TGCTATGAAG ATGAGGGGGT TTATGTAAAC
    ACATATGGAA GGATCACCAA GGATGTAGTT CTACAGTGGG GAGAGATGCC TACATCAGTA
    GCATATATTC GATCCAATCA GACAATGGGC TGGGGAGAGA AGGCCATAGA GATCCGATCT
    GTGGAAACTG GTCACTTGGA TGGTGTGTTC ATGCACAAAA GGGCTCAAAG ACTAAAATTC
    TTGTGTGAAC GCAATGACAA GGTGTTCTTT GCCTCTGTTC GGTCTGGTGG CAGCAGTCAG
    GTTTATTTCA TGACCTTAGG CAGGACTTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860848.1
    CDS75..3899
    Translation

    Target ORF information:

    RefSeq Version XM_017005359.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005359.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT
    GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC
    CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT
    GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC
    GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA
    GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC
    AGCAGTATTG AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC
    AGTGGCAGCT CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT
    CAGAGTGGCT CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA
    TCTCAGCGCC TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC
    CTCAAGCCTG CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA
    GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG
    ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA
    GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA
    ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC
    ACTCTAATCG TCCGCCAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA
    ACAGTGACAT CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA
    GATCCTACCC GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT
    GACACCCCGG AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC
    TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT
    GGCCAAGGGA AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT
    GAGGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT
    TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA
    TGGACAACCG TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA
    ATCAAATTTC TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG
    CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG
    GTGGATCTCA CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA
    TTCCATGCTG TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATGTA
    AGAAAGAACC CACACTCTAT GATCCAGTGT AGCATCAAAC CCCATGCAAT CATCATCCTC
    CCCAATACAG ATGGAATGGA GCTTCTGGTG TGCTATGAAG ATGAGGGGGT TTATGTAAAC
    ACATATGGAA GGATCACCAA GGATGTAGTT CTACAGTGGG GAGAGATGCC TACATCAGTA
    GCATATATTC GATCCAATCA GACAATGGGC TGGGGAGAGA AGGCCATAGA GATCCGATCT
    GTGGAAACTG GTCACTTGGA TGGTGTGTTC ATGCACAAAA GGGCTCAAAG ACTAAAATTC
    TTGTGTGAAC GCAATGACAA GGTGTTCTTT GCCTCTGTTC GGTCTGGTGG CAGCAGTCAG
    GTTTATTTCA TGACCTTAGG CAGGACTTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92783
    Clone ID Related Accession (Same CDS sequence) XM_017005360.1
    Accession Version XM_017005360.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT
    GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC
    CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT
    GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC
    GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA
    GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC
    AGTATTGAGC CCAGGCTTCT GTGGGAGAGA GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT
    GGCAGCTCCT CAGGGTCCAG CAACTCAGGA TCCCAGCCCG GGTCTCACCC TGGGTCTCAG
    AGTGGCTCCG GGGAACGCTT CAGAGTGAGA TCATCATCCA AGTCTGAAGG CTCTCCATCT
    CAGCGCCTGG AAAATGCAGT GAAAAAACCT GAAGATAAAA AGGAAGTTTT CAGACCCCTC
    AAGCCTGCTG GCGAAGTGGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG
    GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG
    GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC
    ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT
    CTAATCGTCC GCCAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGAACAACA
    GTGACATCTG TGGTGGGATT TTCCTGTGAT GGGATGAGAC CAGAAGCCAT AAGGCAAGAT
    CCTACCCGGA AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAC
    ACCCCGGAGA TTCGTAAATA CAAGAAGAGG TTTAACTCTG AGATTCTGTG TGCTGCCTTA
    TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC
    CAAGGGAAGG TCTATCCTCT TATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG
    GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG
    TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG
    ACAACCGTAG GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC
    AAATTTCTGG TGATTGCTTT GAAGAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA
    TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG
    GATCTCACTG TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC
    CATGCTGTTG ATGTGGATTC AGGATCAGTC TATGACATTT ATCTACCAAC ACATGTAAGA
    AAGAACCCAC ACTCTATGAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC
    AATACAGATG GAATGGAGCT TCTGGTGTGC TATGAAGATG AGGGGGTTTA TGTAAACACA
    TATGGAAGGA TCACCAAGGA TGTAGTTCTA CAGTGGGGAG AGATGCCTAC ATCAGTAGCA
    TATATTCGAT CCAATCAGAC AATGGGCTGG GGAGAGAAGG CCATAGAGAT CCGATCTGTG
    GAAACTGGTC ACTTGGATGG TGTGTTCATG CACAAAAGGG CTCAAAGACT AAAATTCTTG
    TGTGAACGCA ATGACAAGGT GTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT
    TATTTCATGA CCTTAGGCAG GACTTCTCTT CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860849.1
    CDS75..3896
    Translation

    Target ORF information:

    RefSeq Version XM_017005360.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005360.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT
    GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC
    CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT
    GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC
    GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA
    GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC
    AGTATTGAGC CCAGGCTTCT GTGGGAGAGA GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT
    GGCAGCTCCT CAGGGTCCAG CAACTCAGGA TCCCAGCCCG GGTCTCACCC TGGGTCTCAG
    AGTGGCTCCG GGGAACGCTT CAGAGTGAGA TCATCATCCA AGTCTGAAGG CTCTCCATCT
    CAGCGCCTGG AAAATGCAGT GAAAAAACCT GAAGATAAAA AGGAAGTTTT CAGACCCCTC
    AAGCCTGCTG GCGAAGTGGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG
    GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG
    GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC
    ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT
    CTAATCGTCC GCCAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGAACAACA
    GTGACATCTG TGGTGGGATT TTCCTGTGAT GGGATGAGAC CAGAAGCCAT AAGGCAAGAT
    CCTACCCGGA AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAC
    ACCCCGGAGA TTCGTAAATA CAAGAAGAGG TTTAACTCTG AGATTCTGTG TGCTGCCTTA
    TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC
    CAAGGGAAGG TCTATCCTCT TATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG
    GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG
    TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG
    ACAACCGTAG GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC
    AAATTTCTGG TGATTGCTTT GAAGAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA
    TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG
    GATCTCACTG TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC
    CATGCTGTTG ATGTGGATTC AGGATCAGTC TATGACATTT ATCTACCAAC ACATGTAAGA
    AAGAACCCAC ACTCTATGAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC
    AATACAGATG GAATGGAGCT TCTGGTGTGC TATGAAGATG AGGGGGTTTA TGTAAACACA
    TATGGAAGGA TCACCAAGGA TGTAGTTCTA CAGTGGGGAG AGATGCCTAC ATCAGTAGCA
    TATATTCGAT CCAATCAGAC AATGGGCTGG GGAGAGAAGG CCATAGAGAT CCGATCTGTG
    GAAACTGGTC ACTTGGATGG TGTGTTCATG CACAAAAGGG CTCAAAGACT AAAATTCTTG
    TGTGAACGCA ATGACAAGGT GTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT
    TATTTCATGA CCTTAGGCAG GACTTCTCTT CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92784
    Clone ID Related Accession (Same CDS sequence) XM_017005361.1
    Accession Version XM_017005361.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT
    GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC
    CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT
    GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC
    GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA
    GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC
    AGCAGTATTG AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC
    AGTGGCAGCT CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT
    CAGAGTGGCT CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA
    TCTCAGCGCC TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC
    CTCAAGCCTG CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA
    GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG
    ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA
    GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA
    ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC
    ACTCTAATCG TCCGCCAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA
    ACAGTGACAT CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA
    GATCCTACCC GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT
    GACACCCCGG AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC
    TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT
    GGCCAAGGGA AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT
    GAGGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT
    TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA
    TGGACAACCG TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA
    ATCAAATTTC TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG
    CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG
    GTGGATCTCA CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA
    TTCCATGCTG TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATATC
    CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT
    CTGGTGTGCT ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT
    GTAGTTCTAC AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA
    ATGGGCTGGG GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT
    GTGTTCATGC ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG
    TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860850.1
    CDS75..3875
    Translation

    Target ORF information:

    RefSeq Version XM_017005361.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005361.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT
    GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC
    CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT
    GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC
    GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA
    GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC
    AGCAGTATTG AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC
    AGTGGCAGCT CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT
    CAGAGTGGCT CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA
    TCTCAGCGCC TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC
    CTCAAGCCTG CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA
    GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG
    ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA
    GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA
    ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC
    ACTCTAATCG TCCGCCAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA
    ACAGTGACAT CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA
    GATCCTACCC GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT
    GACACCCCGG AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC
    TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT
    GGCCAAGGGA AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT
    GAGGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT
    TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA
    TGGACAACCG TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA
    ATCAAATTTC TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG
    CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG
    GTGGATCTCA CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA
    TTCCATGCTG TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATATC
    CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT
    CTGGTGTGCT ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT
    GTAGTTCTAC AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA
    ATGGGCTGGG GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT
    GTGTTCATGC ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG
    TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92785
    Clone ID Related Accession (Same CDS sequence) XM_017005362.1
    Accession Version XM_017005362.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT
    GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC
    CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT
    GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC
    GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA
    GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC
    AGTATTGAGC CCAGGCTTCT GTGGGAGAGA GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT
    GGCAGCTCCT CAGGGTCCAG CAACTCAGGA TCCCAGCCCG GGTCTCACCC TGGGTCTCAG
    AGTGGCTCCG GGGAACGCTT CAGAGTGAGA TCATCATCCA AGTCTGAAGG CTCTCCATCT
    CAGCGCCTGG AAAATGCAGT GAAAAAACCT GAAGATAAAA AGGAAGTTTT CAGACCCCTC
    AAGCCTGCTG GCGAAGTGGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG
    GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG
    GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC
    ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT
    CTAATCGTCC GCCAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGAACAACA
    GTGACATCTG TGGTGGGATT TTCCTGTGAT GGGATGAGAC CAGAAGCCAT AAGGCAAGAT
    CCTACCCGGA AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAC
    ACCCCGGAGA TTCGTAAATA CAAGAAGAGG TTTAACTCTG AGATTCTGTG TGCTGCCTTA
    TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC
    CAAGGGAAGG TCTATCCTCT TATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG
    GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG
    TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG
    ACAACCGTAG GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC
    AAATTTCTGG TGATTGCTTT GAAGAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA
    TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG
    GATCTCACTG TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC
    CATGCTGTTG ATGTGGATTC AGGATCAGTC TATGACATTT ATCTACCAAC ACATATCCAG
    TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG
    GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA
    GTTCTACAGT GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG
    GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG
    TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC
    TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860851.1
    CDS75..3872
    Translation

    Target ORF information:

    RefSeq Version XM_017005362.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005362.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT
    GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC
    CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT
    GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC
    GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA
    GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC
    AGTATTGAGC CCAGGCTTCT GTGGGAGAGA GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT
    GGCAGCTCCT CAGGGTCCAG CAACTCAGGA TCCCAGCCCG GGTCTCACCC TGGGTCTCAG
    AGTGGCTCCG GGGAACGCTT CAGAGTGAGA TCATCATCCA AGTCTGAAGG CTCTCCATCT
    CAGCGCCTGG AAAATGCAGT GAAAAAACCT GAAGATAAAA AGGAAGTTTT CAGACCCCTC
    AAGCCTGCTG GCGAAGTGGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG
    GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG
    GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC
    ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT
    CTAATCGTCC GCCAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGAACAACA
    GTGACATCTG TGGTGGGATT TTCCTGTGAT GGGATGAGAC CAGAAGCCAT AAGGCAAGAT
    CCTACCCGGA AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAC
    ACCCCGGAGA TTCGTAAATA CAAGAAGAGG TTTAACTCTG AGATTCTGTG TGCTGCCTTA
    TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC
    CAAGGGAAGG TCTATCCTCT TATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG
    GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG
    TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG
    ACAACCGTAG GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC
    AAATTTCTGG TGATTGCTTT GAAGAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA
    TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG
    GATCTCACTG TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC
    CATGCTGTTG ATGTGGATTC AGGATCAGTC TATGACATTT ATCTACCAAC ACATATCCAG
    TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG
    GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA
    GTTCTACAGT GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG
    GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG
    TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC
    TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92786
    Clone ID Related Accession (Same CDS sequence) XM_017005363.1
    Accession Version XM_017005363.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3762bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC
    AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC
    CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA
    GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT
    TTCAGACCCC TCAAGCCTGC TGGCGAAGTG GATCTGACCG CACTGGCCAA AGAGCTTCGA
    GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG
    TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC
    TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA
    TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC
    AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT
    GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT
    TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG
    TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG
    ATTTCTCCAT CTAGCGGAAC AACAGTGACA TCTGTGGTGG GATTTTCCTG TGATGGGATG
    AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT
    ACCAACACTA GGCCACAGAG TGACACCCCG GAGATTCGTA AATACAAGAA GAGGTTTAAC
    TCTGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC
    CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTTATCAA CCGAAGACGA
    TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG
    GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA
    GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT
    AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAGAG TTCTGTGGAA
    GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA
    TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCAGGATC AGTCTATGAC
    ATTTATCTAC CAACACATAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC
    AATACAGATG GAATGGAGCT TCTGGTGTGC TATGAAGATG AGGGGGTTTA TGTAAACACA
    TATGGAAGGA TCACCAAGGA TGTAGTTCTA CAGTGGGGAG AGATGCCTAC ATCAGTAGCA
    TATATTCGAT CCAATCAGAC AATGGGCTGG GGAGAGAAGG CCATAGAGAT CCGATCTGTG
    GAAACTGGTC ACTTGGATGG TGTGTTCATG CACAAAAGGG CTCAAAGACT AAAATTCTTG
    TGTGAACGCA ATGACAAGGT GTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT
    TATTTCATGA CCTTAGGCAG GACTTCTCTT CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860852.1
    CDS75..3836
    Translation

    Target ORF information:

    RefSeq Version XM_017005363.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005363.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC
    AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC
    CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA
    GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT
    TTCAGACCCC TCAAGCCTGC TGGCGAAGTG GATCTGACCG CACTGGCCAA AGAGCTTCGA
    GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG
    TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC
    TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA
    TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC
    AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT
    GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT
    TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG
    TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG
    ATTTCTCCAT CTAGCGGAAC AACAGTGACA TCTGTGGTGG GATTTTCCTG TGATGGGATG
    AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT
    ACCAACACTA GGCCACAGAG TGACACCCCG GAGATTCGTA AATACAAGAA GAGGTTTAAC
    TCTGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC
    CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTTATCAA CCGAAGACGA
    TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG
    GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA
    GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT
    AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAGAG TTCTGTGGAA
    GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA
    TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCAGGATC AGTCTATGAC
    ATTTATCTAC CAACACATAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC
    AATACAGATG GAATGGAGCT TCTGGTGTGC TATGAAGATG AGGGGGTTTA TGTAAACACA
    TATGGAAGGA TCACCAAGGA TGTAGTTCTA CAGTGGGGAG AGATGCCTAC ATCAGTAGCA
    TATATTCGAT CCAATCAGAC AATGGGCTGG GGAGAGAAGG CCATAGAGAT CCGATCTGTG
    GAAACTGGTC ACTTGGATGG TGTGTTCATG CACAAAAGGG CTCAAAGACT AAAATTCTTG
    TGTGAACGCA ATGACAAGGT GTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT
    TATTTCATGA CCTTAGGCAG GACTTCTCTT CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92787
    Clone ID Related Accession (Same CDS sequence) XM_017005364.1
    Accession Version XM_017005364.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA
    CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT
    GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC
    TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC
    AGACCCCTCA AGCCTGCTGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG
    GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG
    GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC
    ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT
    CTAATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC
    TTTGCCGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CCTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT
    AGCGGAACAA CAGTGACATC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG
    CCACAGAGTG ACACCCCGGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAGATTCTG
    TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG
    GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTTATCAACC GAAGACGATT TCAACAAATG
    GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG
    AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA
    TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCGTGG
    GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG
    CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCAGGATCAG TCTATGACAT TTATCTACCA
    ACACATATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA
    ATGGAGCTTC TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC
    ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACAT CAGTAGCATA TATTCGATCC
    AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC
    TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT
    GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC
    TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860853.1
    CDS75..3824
    Translation

    Target ORF information:

    RefSeq Version XM_017005364.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005364.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA
    CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT
    GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC
    TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC
    AGACCCCTCA AGCCTGCTGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG
    GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG
    GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC
    ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT
    CTAATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC
    TTTGCCGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CCTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT
    AGCGGAACAA CAGTGACATC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG
    CCACAGAGTG ACACCCCGGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAGATTCTG
    TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG
    GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTTATCAACC GAAGACGATT TCAACAAATG
    GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG
    AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA
    TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCGTGG
    GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG
    CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCAGGATCAG TCTATGACAT TTATCTACCA
    ACACATATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA
    ATGGAGCTTC TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC
    ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACAT CAGTAGCATA TATTCGATCC
    AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC
    TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT
    GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC
    TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92788
    Clone ID Related Accession (Same CDS sequence) XM_017005365.1
    Accession Version XM_017005365.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA
    CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT
    GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC
    TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC
    TCCGGGGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC
    CTGGAAAATG CAGTGAAAAA ACCTGAAGAT AAAAAGGAAG TTTTCAGACC CCTCAAGCCT
    GCTGGCGAAG TGGATCTGAC CGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTACGG
    CCACCTCACA AAGTAACGGA CTACTCCTCA TCCAGTGAGG AGTCGGGGAC GACGGATGAG
    GAGGACGACG ATGTGGAGCA GGAAGGGGCT GACGAGTCCA CCTCAGGACC AGAGGACACC
    AGAGCAGCGT CATCTCTGAA TTTGAGCAAT GGTGAAACGG AATCTGTGAA AACCATGATT
    GTCCATGATG ATGTAGAAAG TGAGCCGGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC
    GTCCGCCAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGCGGAAC AACAGTGACA
    TCTGTGGTGG GATTTTCCTG TGATGGGATG AGACCAGAAG CCATAAGGCA AGATCCTACC
    CGGAAAGGCT CAGTGGTCAA TGTGAATCCT ACCAACACTA GGCCACAGAG TGACACCCCG
    GAGATTCGTA AATACAAGAA GAGGTTTAAC TCTGAGATTC TGTGTGCTGC CTTATGGGGA
    GTGAATTTGC TAGTGGGTAC AGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG
    AAGGTCTATC CTCTTATCAA CCGAAGACGA TTTCAACAAA TGGACGTACT TGAGGGCTTG
    AATGTCTTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG
    TTAAGAAATA AAATACTTCA CAATGATCCA GAAGTTGAGA AGAAGCAGGG ATGGACAACC
    GTAGGGGATT TGGAAGGATG TGTACATTAT AAAGTTGTAA AATATGAAAG AATCAAATTT
    CTGGTGATTG CTTTGAAGAG TTCTGTGGAA GTCTATGCGT GGGCACCAAA GCCATATCAC
    AAATTTATGG CCTTTAAGTC ATTTGGAGAA TTGGTACATA AGCCATTACT GGTGGATCTC
    ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT
    GTTGATGTGG ATTCAGGATC AGTCTATGAC ATTTATCTAC CAACACATAT CCAGTGTAGC
    ATCAAACCCC ATGCAATCAT CATCCTCCCC AATACAGATG GAATGGAGCT TCTGGTGTGC
    TATGAAGATG AGGGGGTTTA TGTAAACACA TATGGAAGGA TCACCAAGGA TGTAGTTCTA
    CAGTGGGGAG AGATGCCTAC ATCAGTAGCA TATATTCGAT CCAATCAGAC AATGGGCTGG
    GGAGAGAAGG CCATAGAGAT CCGATCTGTG GAAACTGGTC ACTTGGATGG TGTGTTCATG
    CACAAAAGGG CTCAAAGACT AAAATTCTTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC
    TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT TATTTCATGA CCTTAGGCAG GACTTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860854.1
    CDS75..3806
    Translation

    Target ORF information:

    RefSeq Version XM_017005365.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005365.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA
    CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT
    GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC
    TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC
    TCCGGGGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC
    CTGGAAAATG CAGTGAAAAA ACCTGAAGAT AAAAAGGAAG TTTTCAGACC CCTCAAGCCT
    GCTGGCGAAG TGGATCTGAC CGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTACGG
    CCACCTCACA AAGTAACGGA CTACTCCTCA TCCAGTGAGG AGTCGGGGAC GACGGATGAG
    GAGGACGACG ATGTGGAGCA GGAAGGGGCT GACGAGTCCA CCTCAGGACC AGAGGACACC
    AGAGCAGCGT CATCTCTGAA TTTGAGCAAT GGTGAAACGG AATCTGTGAA AACCATGATT
    GTCCATGATG ATGTAGAAAG TGAGCCGGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC
    GTCCGCCAGA CTCAGTCCGC TAGTAGCACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGCGGAAC AACAGTGACA
    TCTGTGGTGG GATTTTCCTG TGATGGGATG AGACCAGAAG CCATAAGGCA AGATCCTACC
    CGGAAAGGCT CAGTGGTCAA TGTGAATCCT ACCAACACTA GGCCACAGAG TGACACCCCG
    GAGATTCGTA AATACAAGAA GAGGTTTAAC TCTGAGATTC TGTGTGCTGC CTTATGGGGA
    GTGAATTTGC TAGTGGGTAC AGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG
    AAGGTCTATC CTCTTATCAA CCGAAGACGA TTTCAACAAA TGGACGTACT TGAGGGCTTG
    AATGTCTTGG TGACAATATC TGGCAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG
    TTAAGAAATA AAATACTTCA CAATGATCCA GAAGTTGAGA AGAAGCAGGG ATGGACAACC
    GTAGGGGATT TGGAAGGATG TGTACATTAT AAAGTTGTAA AATATGAAAG AATCAAATTT
    CTGGTGATTG CTTTGAAGAG TTCTGTGGAA GTCTATGCGT GGGCACCAAA GCCATATCAC
    AAATTTATGG CCTTTAAGTC ATTTGGAGAA TTGGTACATA AGCCATTACT GGTGGATCTC
    ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT
    GTTGATGTGG ATTCAGGATC AGTCTATGAC ATTTATCTAC CAACACATAT CCAGTGTAGC
    ATCAAACCCC ATGCAATCAT CATCCTCCCC AATACAGATG GAATGGAGCT TCTGGTGTGC
    TATGAAGATG AGGGGGTTTA TGTAAACACA TATGGAAGGA TCACCAAGGA TGTAGTTCTA
    CAGTGGGGAG AGATGCCTAC ATCAGTAGCA TATATTCGAT CCAATCAGAC AATGGGCTGG
    GGAGAGAAGG CCATAGAGAT CCGATCTGTG GAAACTGGTC ACTTGGATGG TGTGTTCATG
    CACAAAAGGG CTCAAAGACT AAAATTCTTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC
    TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT TATTTCATGA CCTTAGGCAG GACTTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92789
    Clone ID Related Accession (Same CDS sequence) XM_017005366.1
    Accession Version XM_017005366.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC
    CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA
    TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT
    AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA
    GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA
    TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA
    TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT
    GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA
    CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC
    TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA
    GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG
    AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG
    CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA
    CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT
    GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG
    AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG
    TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA
    AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT
    GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA
    CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC
    TATGACATTT ATCTACCAAC ACATGTAAGA AAGAACCCAC ACTCTATGAT CCAGTGTAGC
    ATCAAACCCC ATGCAATCAT CATCCTCCCC AATACAGATG GAATGGAGCT TCTGGTGTGC
    TATGAAGATG AGGGGGTTTA TGTAAACACA TATGGAAGGA TCACCAAGGA TGTAGTTCTA
    CAGTGGGGAG AGATGCCTAC ATCAGTAGCA TATATTCGAT CCAATCAGAC AATGGGCTGG
    GGAGAGAAGG CCATAGAGAT CCGATCTGTG GAAACTGGTC ACTTGGATGG TGTGTTCATG
    CACAAAAGGG CTCAAAGACT AAAATTCTTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC
    TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT TATTTCATGA CCTTAGGCAG GACTTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860855.1
    CDS75..3806
    Translation

    Target ORF information:

    RefSeq Version XM_017005366.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005366.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC
    CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA
    TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT
    AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA
    GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA
    TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA
    TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT
    GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA
    CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC
    TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA
    GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG
    AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG
    CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA
    CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT
    GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG
    AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG
    TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA
    AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT
    GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA
    CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC
    TATGACATTT ATCTACCAAC ACATGTAAGA AAGAACCCAC ACTCTATGAT CCAGTGTAGC
    ATCAAACCCC ATGCAATCAT CATCCTCCCC AATACAGATG GAATGGAGCT TCTGGTGTGC
    TATGAAGATG AGGGGGTTTA TGTAAACACA TATGGAAGGA TCACCAAGGA TGTAGTTCTA
    CAGTGGGGAG AGATGCCTAC ATCAGTAGCA TATATTCGAT CCAATCAGAC AATGGGCTGG
    GGAGAGAAGG CCATAGAGAT CCGATCTGTG GAAACTGGTC ACTTGGATGG TGTGTTCATG
    CACAAAAGGG CTCAAAGACT AAAATTCTTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC
    TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT TATTTCATGA CCTTAGGCAG GACTTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92790
    Clone ID Related Accession (Same CDS sequence) XM_017005367.1
    Accession Version XM_017005367.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC
    CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA
    TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT
    AGCCAGGCAG GACAGAGAAA CTCCACCAGT ATTGAGCCCA GGCTTCTGTG GGAGAGAGTG
    GAGAAGCTGG TGCCCAGACC TGGCAGTGGC AGCTCCTCAG GGTCCAGCAA CTCAGGATCC
    CAGCCCGGGT CTCACCCTGG GTCTCAGAGT GGCTCCGGGG AACGCTTCAG AGTGAGATCA
    TCATCCAAGT CTGAAGGCTC TCCATCTCAG CGCCTGGAAA ATGCAGTGAA AAAACCTGAA
    GATAAAAAGG AAGTTTTCAG ACCCCTCAAG CCTGCTGGCG AAGTGGATCT GACCGCACTG
    GCCAAAGAGC TTCGAGCAGT GGAAGATGTA CGGCCACCTC ACAAAGTAAC GGACTACTCC
    TCATCCAGTG AGGAGTCGGG GACGACGGAT GAGGAGGACG ACGATGTGGA GCAGGAAGGG
    GCTGACGAGT CCACCTCAGG ACCAGAGGAC ACCAGAGCAG CGTCATCTCT GAATTTGAGC
    AATGGTGAAA CGGAATCTGT GAAAACCATG ATTGTCCATG ATGATGTAGA AAGTGAGCCG
    GCCATGACCC CATCCAAGGA GGGCACTCTA ATCGTCCGCC AGACTCAGTC CGCTAGTAGC
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA
    CAGATTTCTC CATCTAGCGG AACAACAGTG ACATCTGTGG TGGGATTTTC CTGTGATGGG
    ATGAGACCAG AAGCCATAAG GCAAGATCCT ACCCGGAAAG GCTCAGTGGT CAATGTGAAT
    CCTACCAACA CTAGGCCACA GAGTGACACC CCGGAGATTC GTAAATACAA GAAGAGGTTT
    AACTCTGAGA TTCTGTGTGC TGCCTTATGG GGAGTGAATT TGCTAGTGGG TACAGAGAGT
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTCT ATCCTCTTAT CAACCGAAGA
    CGATTTCAAC AAATGGACGT ACTTGAGGGC TTGAATGTCT TGGTGACAAT ATCTGGCAAA
    AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAATGAT
    CCAGAAGTTG AGAAGAAGCA GGGATGGACA ACCGTAGGGG ATTTGGAAGG ATGTGTACAT
    TATAAAGTTG TAAAATATGA AAGAATCAAA TTTCTGGTGA TTGCTTTGAA GAGTTCTGTG
    GAAGTCTATG CGTGGGCACC AAAGCCATAT CACAAATTTA TGGCCTTTAA GTCATTTGGA
    GAATTGGTAC ATAAGCCATT ACTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGTTGAAA
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGATTCAGG ATCAGTCTAT
    GACATTTATC TACCAACACA TATCCAGTGT AGCATCAAAC CCCATGCAAT CATCATCCTC
    CCCAATACAG ATGGAATGGA GCTTCTGGTG TGCTATGAAG ATGAGGGGGT TTATGTAAAC
    ACATATGGAA GGATCACCAA GGATGTAGTT CTACAGTGGG GAGAGATGCC TACATCAGTA
    GCATATATTC GATCCAATCA GACAATGGGC TGGGGAGAGA AGGCCATAGA GATCCGATCT
    GTGGAAACTG GTCACTTGGA TGGTGTGTTC ATGCACAAAA GGGCTCAAAG ACTAAAATTC
    TTGTGTGAAC GCAATGACAA GGTGTTCTTT GCCTCTGTTC GGTCTGGTGG CAGCAGTCAG
    GTTTATTTCA TGACCTTAGG CAGGACTTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860856.1
    CDS75..3779
    Translation

    Target ORF information:

    RefSeq Version XM_017005367.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005367.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC
    CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA
    TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT
    AGCCAGGCAG GACAGAGAAA CTCCACCAGT ATTGAGCCCA GGCTTCTGTG GGAGAGAGTG
    GAGAAGCTGG TGCCCAGACC TGGCAGTGGC AGCTCCTCAG GGTCCAGCAA CTCAGGATCC
    CAGCCCGGGT CTCACCCTGG GTCTCAGAGT GGCTCCGGGG AACGCTTCAG AGTGAGATCA
    TCATCCAAGT CTGAAGGCTC TCCATCTCAG CGCCTGGAAA ATGCAGTGAA AAAACCTGAA
    GATAAAAAGG AAGTTTTCAG ACCCCTCAAG CCTGCTGGCG AAGTGGATCT GACCGCACTG
    GCCAAAGAGC TTCGAGCAGT GGAAGATGTA CGGCCACCTC ACAAAGTAAC GGACTACTCC
    TCATCCAGTG AGGAGTCGGG GACGACGGAT GAGGAGGACG ACGATGTGGA GCAGGAAGGG
    GCTGACGAGT CCACCTCAGG ACCAGAGGAC ACCAGAGCAG CGTCATCTCT GAATTTGAGC
    AATGGTGAAA CGGAATCTGT GAAAACCATG ATTGTCCATG ATGATGTAGA AAGTGAGCCG
    GCCATGACCC CATCCAAGGA GGGCACTCTA ATCGTCCGCC AGACTCAGTC CGCTAGTAGC
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA
    CAGATTTCTC CATCTAGCGG AACAACAGTG ACATCTGTGG TGGGATTTTC CTGTGATGGG
    ATGAGACCAG AAGCCATAAG GCAAGATCCT ACCCGGAAAG GCTCAGTGGT CAATGTGAAT
    CCTACCAACA CTAGGCCACA GAGTGACACC CCGGAGATTC GTAAATACAA GAAGAGGTTT
    AACTCTGAGA TTCTGTGTGC TGCCTTATGG GGAGTGAATT TGCTAGTGGG TACAGAGAGT
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTCT ATCCTCTTAT CAACCGAAGA
    CGATTTCAAC AAATGGACGT ACTTGAGGGC TTGAATGTCT TGGTGACAAT ATCTGGCAAA
    AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAATGAT
    CCAGAAGTTG AGAAGAAGCA GGGATGGACA ACCGTAGGGG ATTTGGAAGG ATGTGTACAT
    TATAAAGTTG TAAAATATGA AAGAATCAAA TTTCTGGTGA TTGCTTTGAA GAGTTCTGTG
    GAAGTCTATG CGTGGGCACC AAAGCCATAT CACAAATTTA TGGCCTTTAA GTCATTTGGA
    GAATTGGTAC ATAAGCCATT ACTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGTTGAAA
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGATTCAGG ATCAGTCTAT
    GACATTTATC TACCAACACA TATCCAGTGT AGCATCAAAC CCCATGCAAT CATCATCCTC
    CCCAATACAG ATGGAATGGA GCTTCTGGTG TGCTATGAAG ATGAGGGGGT TTATGTAAAC
    ACATATGGAA GGATCACCAA GGATGTAGTT CTACAGTGGG GAGAGATGCC TACATCAGTA
    GCATATATTC GATCCAATCA GACAATGGGC TGGGGAGAGA AGGCCATAGA GATCCGATCT
    GTGGAAACTG GTCACTTGGA TGGTGTGTTC ATGCACAAAA GGGCTCAAAG ACTAAAATTC
    TTGTGTGAAC GCAATGACAA GGTGTTCTTT GCCTCTGTTC GGTCTGGTGG CAGCAGTCAG
    GTTTATTTCA TGACCTTAGG CAGGACTTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92791
    Clone ID Related Accession (Same CDS sequence) XM_017005368.1
    Accession Version XM_017005368.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X34
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG TATTGAGCCC AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG
    GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA GGGTCCAGCA ACTCAGGATC CCAGCCCGGG
    TCTCACCCTG GGTCTCAGAG TGGCTCCGGG GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGCT CTCCATCTCA GCGCCTGGAA AATGCAGTGA AAAAACCTGA AGATAAAAAG
    GAAGTTTTCA GACCCCTCAA GCCTGCTGGC GAAGTGGATC TGACCGCACT GGCCAAAGAG
    CTTCGAGCAG TGGAAGATGT ACGGCCACCT CACAAAGTAA CGGACTACTC CTCATCCAGT
    GAGGAGTCGG GGACGACGGA TGAGGAGGAC GACGATGTGG AGCAGGAAGG GGCTGACGAG
    TCCACCTCAG GACCAGAGGA CACCAGAGCA GCGTCATCTC TGAATTTGAG CAATGGTGAA
    ACGGAATCTG TGAAAACCAT GATTGTCCAT GATGATGTAG AAAGTGAGCC GGCCATGACC
    CCATCCAAGG AGGGCACTCT AATCGTCCGC CAGACTCAGT CCGCTAGTAG CACACTCCAG
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT
    CCATCTAGCG GAACAACAGT GACATCTGTG GTGGGATTTT CCTGTGATGG GATGAGACCA
    GAAGCCATAA GGCAAGATCC TACCCGGAAA GGCTCAGTGG TCAATGTGAA TCCTACCAAC
    ACTAGGCCAC AGAGTGACAC CCCGGAGATT CGTAAATACA AGAAGAGGTT TAACTCTGAG
    ATTCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTGG GTACAGAGAG TGGCCTGATG
    CTGCTGGACA GAAGTGGCCA AGGGAAGGTC TATCCTCTTA TCAACCGAAG ACGATTTCAA
    CAAATGGACG TACTTGAGGG CTTGAATGTC TTGGTGACAA TATCTGGCAA AAAGGATAAG
    TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAATGA TCCAGAAGTT
    GAGAAGAAGC AGGGATGGAC AACCGTAGGG GATTTGGAAG GATGTGTACA TTATAAAGTT
    GTAAAATATG AAAGAATCAA ATTTCTGGTG ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT
    GCGTGGGCAC CAAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAATTGGTA
    CATAAGCCAT TACTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGTTGAA AGTGATCTAT
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCAG GATCAGTCTA TGACATTTAT
    CTACCAACAC ATATCCAGTG TAGCATCAAA CCCCATGCAA TCATCATCCT CCCCAATACA
    GATGGAATGG AGCTTCTGGT GTGCTATGAA GATGAGGGGG TTTATGTAAA CACATATGGA
    AGGATCACCA AGGATGTAGT TCTACAGTGG GGAGAGATGC CTACATCAGT AGCATATATT
    CGATCCAATC AGACAATGGG CTGGGGAGAG AAGGCCATAG AGATCCGATC TGTGGAAACT
    GGTCACTTGG ATGGTGTGTT CATGCACAAA AGGGCTCAAA GACTAAAATT CTTGTGTGAA
    CGCAATGACA AGGTGTTCTT TGCCTCTGTT CGGTCTGGTG GCAGCAGTCA GGTTTATTTC
    ATGACCTTAG GCAGGACTTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860857.1
    CDS75..3710
    Translation

    Target ORF information:

    RefSeq Version XM_017005368.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X34, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005368.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG TATTGAGCCC AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG
    GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA GGGTCCAGCA ACTCAGGATC CCAGCCCGGG
    TCTCACCCTG GGTCTCAGAG TGGCTCCGGG GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGCT CTCCATCTCA GCGCCTGGAA AATGCAGTGA AAAAACCTGA AGATAAAAAG
    GAAGTTTTCA GACCCCTCAA GCCTGCTGGC GAAGTGGATC TGACCGCACT GGCCAAAGAG
    CTTCGAGCAG TGGAAGATGT ACGGCCACCT CACAAAGTAA CGGACTACTC CTCATCCAGT
    GAGGAGTCGG GGACGACGGA TGAGGAGGAC GACGATGTGG AGCAGGAAGG GGCTGACGAG
    TCCACCTCAG GACCAGAGGA CACCAGAGCA GCGTCATCTC TGAATTTGAG CAATGGTGAA
    ACGGAATCTG TGAAAACCAT GATTGTCCAT GATGATGTAG AAAGTGAGCC GGCCATGACC
    CCATCCAAGG AGGGCACTCT AATCGTCCGC CAGACTCAGT CCGCTAGTAG CACACTCCAG
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT
    CCATCTAGCG GAACAACAGT GACATCTGTG GTGGGATTTT CCTGTGATGG GATGAGACCA
    GAAGCCATAA GGCAAGATCC TACCCGGAAA GGCTCAGTGG TCAATGTGAA TCCTACCAAC
    ACTAGGCCAC AGAGTGACAC CCCGGAGATT CGTAAATACA AGAAGAGGTT TAACTCTGAG
    ATTCTGTGTG CTGCCTTATG GGGAGTGAAT TTGCTAGTGG GTACAGAGAG TGGCCTGATG
    CTGCTGGACA GAAGTGGCCA AGGGAAGGTC TATCCTCTTA TCAACCGAAG ACGATTTCAA
    CAAATGGACG TACTTGAGGG CTTGAATGTC TTGGTGACAA TATCTGGCAA AAAGGATAAG
    TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAATGA TCCAGAAGTT
    GAGAAGAAGC AGGGATGGAC AACCGTAGGG GATTTGGAAG GATGTGTACA TTATAAAGTT
    GTAAAATATG AAAGAATCAA ATTTCTGGTG ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT
    GCGTGGGCAC CAAAGCCATA TCACAAATTT ATGGCCTTTA AGTCATTTGG AGAATTGGTA
    CATAAGCCAT TACTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGTTGAA AGTGATCTAT
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGATTCAG GATCAGTCTA TGACATTTAT
    CTACCAACAC ATATCCAGTG TAGCATCAAA CCCCATGCAA TCATCATCCT CCCCAATACA
    GATGGAATGG AGCTTCTGGT GTGCTATGAA GATGAGGGGG TTTATGTAAA CACATATGGA
    AGGATCACCA AGGATGTAGT TCTACAGTGG GGAGAGATGC CTACATCAGT AGCATATATT
    CGATCCAATC AGACAATGGG CTGGGGAGAG AAGGCCATAG AGATCCGATC TGTGGAAACT
    GGTCACTTGG ATGGTGTGTT CATGCACAAA AGGGCTCAAA GACTAAAATT CTTGTGTGAA
    CGCAATGACA AGGTGTTCTT TGCCTCTGTT CGGTCTGGTG GCAGCAGTCA GGTTTATTTC
    ATGACCTTAG GCAGGACTTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92792
    Clone ID Related Accession (Same CDS sequence) XM_017005369.1
    Accession Version XM_017005369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3591bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X36
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA
    CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT
    GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC
    TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC
    AGACCCCTCA AGCCTGCTGG CGAAGTGGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG
    GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA
    GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT
    GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG
    GAGGGCACTC TAATCGTCCG CCAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGATTAC TACAGATTTC TCCATCTAGC
    GGAACAACAG TGACATCTGT GGTGGGATTT TCCTGTGATG GGATGAGACC AGAAGCCATA
    AGGCAAGATC CTACCCGGAA AGGCTCAGTG GTCAATGTGA ATCCTACCAA CACTAGGCCA
    CAGAGTGACA CCCCGGAGAT TCGTAAATAC AAGAAGAGGT TTAACTCTGA GATTCTGTGT
    GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG GGTACAGAGA GTGGCCTGAT GCTGCTGGAC
    AGAAGTGGCC AAGGGAAGGT CTATCCTCTT ATCAACCGAA GACGATTTCA ACAAATGGAC
    GTACTTGAGG GCTTGAATGT CTTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC
    TACTATTTGT CCTGGTTAAG AAATAAAATA CTTCACAATG ATCCAGAAGT TGAGAAGAAG
    CAGGGATGGA CAACCGTAGG GGATTTGGAA GGATGTGTAC ATTATAAAGT TGTAAAATAT
    GAAAGAATCA AATTTCTGGT GATTGCTTTG AAGAGTTCTG TGGAAGTCTA TGCGTGGGCA
    CCAAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAATTGGT ACATAAGCCA
    TTACTGGTGG ATCTCACTGT TGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT
    GCTGGATTCC ATGCTGTTGA TGTGGATTCA GGATCAGTCT ATGACATTTA TCTACCAACA
    CATGTAAGAA AGAACCCACA CTCTATGATC CAGTGTAGCA TCAAACCCCA TGCAATCATC
    ATCCTCCCCA ATACAGATGG AATGGAGCTT CTGGTGTGCT ATGAAGATGA GGGGGTTTAT
    GTAAACACAT ATGGAAGGAT CACCAAGGAT GTAGTTCTAC AGTGGGGAGA GATGCCTACA
    TCAGTAGCAT ATATTCGATC CAATCAGACA ATGGGCTGGG GAGAGAAGGC CATAGAGATC
    CGATCTGTGG AAACTGGTCA CTTGGATGGT GTGTTCATGC ACAAAAGGGC TCAAAGACTA
    AAATTCTTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC
    AGTCAGGTTT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860858.1
    CDS75..3665
    Translation

    Target ORF information:

    RefSeq Version XM_017005369.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X36, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA
    CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT
    GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC
    TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC
    AGACCCCTCA AGCCTGCTGG CGAAGTGGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG
    GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA
    GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT
    GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG
    GAGGGCACTC TAATCGTCCG CCAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGATTAC TACAGATTTC TCCATCTAGC
    GGAACAACAG TGACATCTGT GGTGGGATTT TCCTGTGATG GGATGAGACC AGAAGCCATA
    AGGCAAGATC CTACCCGGAA AGGCTCAGTG GTCAATGTGA ATCCTACCAA CACTAGGCCA
    CAGAGTGACA CCCCGGAGAT TCGTAAATAC AAGAAGAGGT TTAACTCTGA GATTCTGTGT
    GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG GGTACAGAGA GTGGCCTGAT GCTGCTGGAC
    AGAAGTGGCC AAGGGAAGGT CTATCCTCTT ATCAACCGAA GACGATTTCA ACAAATGGAC
    GTACTTGAGG GCTTGAATGT CTTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC
    TACTATTTGT CCTGGTTAAG AAATAAAATA CTTCACAATG ATCCAGAAGT TGAGAAGAAG
    CAGGGATGGA CAACCGTAGG GGATTTGGAA GGATGTGTAC ATTATAAAGT TGTAAAATAT
    GAAAGAATCA AATTTCTGGT GATTGCTTTG AAGAGTTCTG TGGAAGTCTA TGCGTGGGCA
    CCAAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAATTGGT ACATAAGCCA
    TTACTGGTGG ATCTCACTGT TGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT
    GCTGGATTCC ATGCTGTTGA TGTGGATTCA GGATCAGTCT ATGACATTTA TCTACCAACA
    CATGTAAGAA AGAACCCACA CTCTATGATC CAGTGTAGCA TCAAACCCCA TGCAATCATC
    ATCCTCCCCA ATACAGATGG AATGGAGCTT CTGGTGTGCT ATGAAGATGA GGGGGTTTAT
    GTAAACACAT ATGGAAGGAT CACCAAGGAT GTAGTTCTAC AGTGGGGAGA GATGCCTACA
    TCAGTAGCAT ATATTCGATC CAATCAGACA ATGGGCTGGG GAGAGAAGGC CATAGAGATC
    CGATCTGTGG AAACTGGTCA CTTGGATGGT GTGTTCATGC ACAAAAGGGC TCAAAGACTA
    AAATTCTTGT GTGAACGCAA TGACAAGGTG TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC
    AGTCAGGTTT ATTTCATGAC CTTAGGCAGG ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92793
    Clone ID Related Accession (Same CDS sequence) XM_017005370.1
    Accession Version XM_017005370.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X38
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA
    CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT
    GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC
    TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC
    AGACCCCTCA AGCCTGCTGG CGAAGTGGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG
    GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA
    GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT
    GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG
    GAGGGCACTC TAATCGTCCG CCAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGATTAC TACAGATTTC TCCATCTAGC
    GGAACAACAG TGACATCTGT GGTGGGATTT TCCTGTGATG GGATGAGACC AGAAGCCATA
    AGGCAAGATC CTACCCGGAA AGGCTCAGTG GTCAATGTGA ATCCTACCAA CACTAGGCCA
    CAGAGTGACA CCCCGGAGAT TCGTAAATAC AAGAAGAGGT TTAACTCTGA GATTCTGTGT
    GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG GGTACAGAGA GTGGCCTGAT GCTGCTGGAC
    AGAAGTGGCC AAGGGAAGGT CTATCCTCTT ATCAACCGAA GACGATTTCA ACAAATGGAC
    GTACTTGAGG GCTTGAATGT CTTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC
    TACTATTTGT CCTGGTTAAG AAATAAAATA CTTCACAATG ATCCAGAAGT TGAGAAGAAG
    CAGGGATGGA CAACCGTAGG GGATTTGGAA GGATGTGTAC ATTATAAAGT TGTAAAATAT
    GAAAGAATCA AATTTCTGGT GATTGCTTTG AAGAGTTCTG TGGAAGTCTA TGCGTGGGCA
    CCAAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAATTGGT ACATAAGCCA
    TTACTGGTGG ATCTCACTGT TGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT
    GCTGGATTCC ATGCTGTTGA TGTGGATTCA GGATCAGTCT ATGACATTTA TCTACCAACA
    CATATCCAGT GTAGCATCAA ACCCCATGCA ATCATCATCC TCCCCAATAC AGATGGAATG
    GAGCTTCTGG TGTGCTATGA AGATGAGGGG GTTTATGTAA ACACATATGG AAGGATCACC
    AAGGATGTAG TTCTACAGTG GGGAGAGATG CCTACATCAG TAGCATATAT TCGATCCAAT
    CAGACAATGG GCTGGGGAGA GAAGGCCATA GAGATCCGAT CTGTGGAAAC TGGTCACTTG
    GATGGTGTGT TCATGCACAA AAGGGCTCAA AGACTAAAAT TCTTGTGTGA ACGCAATGAC
    AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT GGCAGCAGTC AGGTTTATTT CATGACCTTA
    GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860859.1
    CDS75..3641
    Translation

    Target ORF information:

    RefSeq Version XM_017005370.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X38, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005370.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA
    CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT
    GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC
    TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC
    AGACCCCTCA AGCCTGCTGG CGAAGTGGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG
    GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA
    GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT
    GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG
    GAGGGCACTC TAATCGTCCG CCAGACTCAG TCCGCTAGTA GCACACTCCA GAAACACAAA
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGATTAC TACAGATTTC TCCATCTAGC
    GGAACAACAG TGACATCTGT GGTGGGATTT TCCTGTGATG GGATGAGACC AGAAGCCATA
    AGGCAAGATC CTACCCGGAA AGGCTCAGTG GTCAATGTGA ATCCTACCAA CACTAGGCCA
    CAGAGTGACA CCCCGGAGAT TCGTAAATAC AAGAAGAGGT TTAACTCTGA GATTCTGTGT
    GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG GGTACAGAGA GTGGCCTGAT GCTGCTGGAC
    AGAAGTGGCC AAGGGAAGGT CTATCCTCTT ATCAACCGAA GACGATTTCA ACAAATGGAC
    GTACTTGAGG GCTTGAATGT CTTGGTGACA ATATCTGGCA AAAAGGATAA GTTACGTGTC
    TACTATTTGT CCTGGTTAAG AAATAAAATA CTTCACAATG ATCCAGAAGT TGAGAAGAAG
    CAGGGATGGA CAACCGTAGG GGATTTGGAA GGATGTGTAC ATTATAAAGT TGTAAAATAT
    GAAAGAATCA AATTTCTGGT GATTGCTTTG AAGAGTTCTG TGGAAGTCTA TGCGTGGGCA
    CCAAAGCCAT ATCACAAATT TATGGCCTTT AAGTCATTTG GAGAATTGGT ACATAAGCCA
    TTACTGGTGG ATCTCACTGT TGAGGAAGGC CAGAGGTTGA AAGTGATCTA TGGATCCTGT
    GCTGGATTCC ATGCTGTTGA TGTGGATTCA GGATCAGTCT ATGACATTTA TCTACCAACA
    CATATCCAGT GTAGCATCAA ACCCCATGCA ATCATCATCC TCCCCAATAC AGATGGAATG
    GAGCTTCTGG TGTGCTATGA AGATGAGGGG GTTTATGTAA ACACATATGG AAGGATCACC
    AAGGATGTAG TTCTACAGTG GGGAGAGATG CCTACATCAG TAGCATATAT TCGATCCAAT
    CAGACAATGG GCTGGGGAGA GAAGGCCATA GAGATCCGAT CTGTGGAAAC TGGTCACTTG
    GATGGTGTGT TCATGCACAA AAGGGCTCAA AGACTAAAAT TCTTGTGTGA ACGCAATGAC
    AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT GGCAGCAGTC AGGTTTATTT CATGACCTTA
    GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92794
    Clone ID Related Accession (Same CDS sequence) XM_017005371.1
    Accession Version XM_017005371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3561bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X39
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC
    AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC
    CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA
    GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT
    TTCAGACCCC TCAAGCCTGC TGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA
    GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG
    ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA
    GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA
    ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC
    ACTCTAATCG TCCGCCAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA
    ACAGTGACAT CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA
    GATCCTACCC GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT
    GACACCCCGG AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC
    TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT
    GGCCAAGGGA AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT
    GAGGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT
    TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA
    TGGACAACCG TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA
    ATCAAATTTC TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG
    CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG
    GTGGATCTCA CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA
    TTCCATGCTG TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATATC
    CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT
    CTGGTGTGCT ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT
    GTAGTTCTAC AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA
    ATGGGCTGGG GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT
    GTGTTCATGC ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG
    TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860860.1
    CDS75..3635
    Translation

    Target ORF information:

    RefSeq Version XM_017005371.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X39, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC
    AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC
    CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA
    GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT
    TTCAGACCCC TCAAGCCTGC TGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA
    GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG
    ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA
    GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA
    ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC
    ACTCTAATCG TCCGCCAGAC TCAGTCCGCT AGTAGCACAC TCCAGAAACA CAAATCTTCC
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGAACA
    ACAGTGACAT CTGTGGTGGG ATTTTCCTGT GATGGGATGA GACCAGAAGC CATAAGGCAA
    GATCCTACCC GGAAAGGCTC AGTGGTCAAT GTGAATCCTA CCAACACTAG GCCACAGAGT
    GACACCCCGG AGATTCGTAA ATACAAGAAG AGGTTTAACT CTGAGATTCT GTGTGCTGCC
    TTATGGGGAG TGAATTTGCT AGTGGGTACA GAGAGTGGCC TGATGCTGCT GGACAGAAGT
    GGCCAAGGGA AGGTCTATCC TCTTATCAAC CGAAGACGAT TTCAACAAAT GGACGTACTT
    GAGGGCTTGA ATGTCTTGGT GACAATATCT GGCAAAAAGG ATAAGTTACG TGTCTACTAT
    TTGTCCTGGT TAAGAAATAA AATACTTCAC AATGATCCAG AAGTTGAGAA GAAGCAGGGA
    TGGACAACCG TAGGGGATTT GGAAGGATGT GTACATTATA AAGTTGTAAA ATATGAAAGA
    ATCAAATTTC TGGTGATTGC TTTGAAGAGT TCTGTGGAAG TCTATGCGTG GGCACCAAAG
    CCATATCACA AATTTATGGC CTTTAAGTCA TTTGGAGAAT TGGTACATAA GCCATTACTG
    GTGGATCTCA CTGTTGAGGA AGGCCAGAGG TTGAAAGTGA TCTATGGATC CTGTGCTGGA
    TTCCATGCTG TTGATGTGGA TTCAGGATCA GTCTATGACA TTTATCTACC AACACATATC
    CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT
    CTGGTGTGCT ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT
    GTAGTTCTAC AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA
    ATGGGCTGGG GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT
    GTGTTCATGC ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG
    TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92795
    Clone ID Related Accession (Same CDS sequence) XM_017005372.1
    Accession Version XM_017005372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X41
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG
    AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT
    CTGACCGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TACGGCCACC TCACAAAGTA
    ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG
    GAGCAGGAAG GGGCTGACGA GTCCACCTCA GGACCAGAGG ACACCAGAGC AGCGTCATCT
    CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA
    GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGATTAC TACAGATTTC TCCATCTAGC GGAACAACAG TGACATCTGT GGTGGGATTT
    TCCTGTGATG GGATGAGACC AGAAGCCATA AGGCAAGATC CTACCCGGAA AGGCTCAGTG
    GTCAATGTGA ATCCTACCAA CACTAGGCCA CAGAGTGACA CCCCGGAGAT TCGTAAATAC
    AAGAAGAGGT TTAACTCTGA GATTCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG
    GGTACAGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT CTATCCTCTT
    ATCAACCGAA GACGATTTCA ACAAATGGAC GTACTTGAGG GCTTGAATGT CTTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA
    CTTCACAATG ATCCAGAAGT TGAGAAGAAG CAGGGATGGA CAACCGTAGG GGATTTGGAA
    GGATGTGTAC ATTATAAAGT TGTAAAATAT GAAAGAATCA AATTTCTGGT GATTGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCGTGGGCA CCAAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAATTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT TGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCA
    GGATCAGTCT ATGACATTTA TCTACCAACA CATGTAAGAA AGAACCCACA CTCTATGATC
    CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT
    CTGGTGTGCT ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT
    GTAGTTCTAC AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA
    ATGGGCTGGG GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT
    GTGTTCATGC ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG
    TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860861.1
    CDS75..3575
    Translation

    Target ORF information:

    RefSeq Version XM_017005372.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X41, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG
    AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT
    CTGACCGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TACGGCCACC TCACAAAGTA
    ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG
    GAGCAGGAAG GGGCTGACGA GTCCACCTCA GGACCAGAGG ACACCAGAGC AGCGTCATCT
    CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA
    GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGATTAC TACAGATTTC TCCATCTAGC GGAACAACAG TGACATCTGT GGTGGGATTT
    TCCTGTGATG GGATGAGACC AGAAGCCATA AGGCAAGATC CTACCCGGAA AGGCTCAGTG
    GTCAATGTGA ATCCTACCAA CACTAGGCCA CAGAGTGACA CCCCGGAGAT TCGTAAATAC
    AAGAAGAGGT TTAACTCTGA GATTCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG
    GGTACAGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT CTATCCTCTT
    ATCAACCGAA GACGATTTCA ACAAATGGAC GTACTTGAGG GCTTGAATGT CTTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA
    CTTCACAATG ATCCAGAAGT TGAGAAGAAG CAGGGATGGA CAACCGTAGG GGATTTGGAA
    GGATGTGTAC ATTATAAAGT TGTAAAATAT GAAAGAATCA AATTTCTGGT GATTGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCGTGGGCA CCAAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAATTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT TGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCA
    GGATCAGTCT ATGACATTTA TCTACCAACA CATGTAAGAA AGAACCCACA CTCTATGATC
    CAGTGTAGCA TCAAACCCCA TGCAATCATC ATCCTCCCCA ATACAGATGG AATGGAGCTT
    CTGGTGTGCT ATGAAGATGA GGGGGTTTAT GTAAACACAT ATGGAAGGAT CACCAAGGAT
    GTAGTTCTAC AGTGGGGAGA GATGCCTACA TCAGTAGCAT ATATTCGATC CAATCAGACA
    ATGGGCTGGG GAGAGAAGGC CATAGAGATC CGATCTGTGG AAACTGGTCA CTTGGATGGT
    GTGTTCATGC ACAAAAGGGC TCAAAGACTA AAATTCTTGT GTGAACGCAA TGACAAGGTG
    TTCTTTGCCT CTGTTCGGTC TGGTGGCAGC AGTCAGGTTT ATTTCATGAC CTTAGGCAGG
    ACTTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92796
    Clone ID Related Accession (Same CDS sequence) XM_017005373.1
    Accession Version XM_017005373.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X42
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG
    AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGA TCTGACCGCA
    CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC
    TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA
    GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG
    AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG
    CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA
    CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT
    GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG
    AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG
    TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA
    AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT
    GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA
    CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC
    TATGACATTT ATCTACCAAC ACATGTAAGA AAGAACCCAC ACTCTATGAT CCAGTGTAGC
    ATCAAACCCC ATGCAATCAT CATCCTCCCC AATACAGATG GAATGGAGCT TCTGGTGTGC
    TATGAAGATG AGGGGGTTTA TGTAAACACA TATGGAAGGA TCACCAAGGA TGTAGTTCTA
    CAGTGGGGAG AGATGCCTAC ATCAGTAGCA TATATTCGAT CCAATCAGAC AATGGGCTGG
    GGAGAGAAGG CCATAGAGAT CCGATCTGTG GAAACTGGTC ACTTGGATGG TGTGTTCATG
    CACAAAAGGG CTCAAAGACT AAAATTCTTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC
    TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT TATTTCATGA CCTTAGGCAG GACTTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860862.1
    CDS75..3566
    Translation

    Target ORF information:

    RefSeq Version XM_017005373.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X42, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG
    AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGA TCTGACCGCA
    CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC
    TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA
    GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG
    AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG
    CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA
    CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT
    GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG
    AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG
    TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA
    AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT
    GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA
    CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC
    TATGACATTT ATCTACCAAC ACATGTAAGA AAGAACCCAC ACTCTATGAT CCAGTGTAGC
    ATCAAACCCC ATGCAATCAT CATCCTCCCC AATACAGATG GAATGGAGCT TCTGGTGTGC
    TATGAAGATG AGGGGGTTTA TGTAAACACA TATGGAAGGA TCACCAAGGA TGTAGTTCTA
    CAGTGGGGAG AGATGCCTAC ATCAGTAGCA TATATTCGAT CCAATCAGAC AATGGGCTGG
    GGAGAGAAGG CCATAGAGAT CCGATCTGTG GAAACTGGTC ACTTGGATGG TGTGTTCATG
    CACAAAAGGG CTCAAAGACT AAAATTCTTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC
    TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT TATTTCATGA CCTTAGGCAG GACTTCTCTT
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92797
    Clone ID Related Accession (Same CDS sequence) XM_017005374.1
    Accession Version XM_017005374.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X43
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG
    AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT
    CTGACCGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TACGGCCACC TCACAAAGTA
    ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG
    GAGCAGGAAG GGGCTGACGA GTCCACCTCA GGACCAGAGG ACACCAGAGC AGCGTCATCT
    CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA
    GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGATTAC TACAGATTTC TCCATCTAGC GGAACAACAG TGACATCTGT GGTGGGATTT
    TCCTGTGATG GGATGAGACC AGAAGCCATA AGGCAAGATC CTACCCGGAA AGGCTCAGTG
    GTCAATGTGA ATCCTACCAA CACTAGGCCA CAGAGTGACA CCCCGGAGAT TCGTAAATAC
    AAGAAGAGGT TTAACTCTGA GATTCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG
    GGTACAGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT CTATCCTCTT
    ATCAACCGAA GACGATTTCA ACAAATGGAC GTACTTGAGG GCTTGAATGT CTTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA
    CTTCACAATG ATCCAGAAGT TGAGAAGAAG CAGGGATGGA CAACCGTAGG GGATTTGGAA
    GGATGTGTAC ATTATAAAGT TGTAAAATAT GAAAGAATCA AATTTCTGGT GATTGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCGTGGGCA CCAAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAATTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT TGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCA
    GGATCAGTCT ATGACATTTA TCTACCAACA CATATCCAGT GTAGCATCAA ACCCCATGCA
    ATCATCATCC TCCCCAATAC AGATGGAATG GAGCTTCTGG TGTGCTATGA AGATGAGGGG
    GTTTATGTAA ACACATATGG AAGGATCACC AAGGATGTAG TTCTACAGTG GGGAGAGATG
    CCTACATCAG TAGCATATAT TCGATCCAAT CAGACAATGG GCTGGGGAGA GAAGGCCATA
    GAGATCCGAT CTGTGGAAAC TGGTCACTTG GATGGTGTGT TCATGCACAA AAGGGCTCAA
    AGACTAAAAT TCTTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT
    GGCAGCAGTC AGGTTTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860863.1
    CDS75..3551
    Translation

    Target ORF information:

    RefSeq Version XM_017005374.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X43, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005374.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG
    AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT
    CTGACCGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TACGGCCACC TCACAAAGTA
    ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG
    GAGCAGGAAG GGGCTGACGA GTCCACCTCA GGACCAGAGG ACACCAGAGC AGCGTCATCT
    CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA
    GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGACTCAG
    TCCGCTAGTA GCACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC
    CCCAGATTAC TACAGATTTC TCCATCTAGC GGAACAACAG TGACATCTGT GGTGGGATTT
    TCCTGTGATG GGATGAGACC AGAAGCCATA AGGCAAGATC CTACCCGGAA AGGCTCAGTG
    GTCAATGTGA ATCCTACCAA CACTAGGCCA CAGAGTGACA CCCCGGAGAT TCGTAAATAC
    AAGAAGAGGT TTAACTCTGA GATTCTGTGT GCTGCCTTAT GGGGAGTGAA TTTGCTAGTG
    GGTACAGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT CTATCCTCTT
    ATCAACCGAA GACGATTTCA ACAAATGGAC GTACTTGAGG GCTTGAATGT CTTGGTGACA
    ATATCTGGCA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA
    CTTCACAATG ATCCAGAAGT TGAGAAGAAG CAGGGATGGA CAACCGTAGG GGATTTGGAA
    GGATGTGTAC ATTATAAAGT TGTAAAATAT GAAAGAATCA AATTTCTGGT GATTGCTTTG
    AAGAGTTCTG TGGAAGTCTA TGCGTGGGCA CCAAAGCCAT ATCACAAATT TATGGCCTTT
    AAGTCATTTG GAGAATTGGT ACATAAGCCA TTACTGGTGG ATCTCACTGT TGAGGAAGGC
    CAGAGGTTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGATTCA
    GGATCAGTCT ATGACATTTA TCTACCAACA CATATCCAGT GTAGCATCAA ACCCCATGCA
    ATCATCATCC TCCCCAATAC AGATGGAATG GAGCTTCTGG TGTGCTATGA AGATGAGGGG
    GTTTATGTAA ACACATATGG AAGGATCACC AAGGATGTAG TTCTACAGTG GGGAGAGATG
    CCTACATCAG TAGCATATAT TCGATCCAAT CAGACAATGG GCTGGGGAGA GAAGGCCATA
    GAGATCCGAT CTGTGGAAAC TGGTCACTTG GATGGTGTGT TCATGCACAA AAGGGCTCAA
    AGACTAAAAT TCTTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT
    GGCAGCAGTC AGGTTTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu92798
    Clone ID Related Accession (Same CDS sequence) XM_017005375.1
    Accession Version XM_017005375.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X44
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG
    AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGA TCTGACCGCA
    CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC
    TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA
    GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG
    AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG
    CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA
    CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT
    GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG
    AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG
    TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA
    AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT
    GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA
    CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC
    TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA AACCCCATGC AATCATCATC
    CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA
    AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA
    GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA
    TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA
    TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT
    CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860864.1
    CDS75..3542
    Translation

    Target ORF information:

    RefSeq Version XM_017005375.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X44, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005375.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG
    AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGA TCTGACCGCA
    CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC
    TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA
    GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG
    AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG
    CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA
    CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT
    GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG
    AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG
    TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA
    AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT
    GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA
    CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC
    TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA AACCCCATGC AATCATCATC
    CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA
    AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA
    GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA
    TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA
    TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT
    CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20375
    Clone ID Related Accession (Same CDS sequence) NM_145687.3 , NM_145687.4
    Accession Version NM_145687.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-09-29
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB086475.1, AB014587.1, AC005035.2 and BU625485.1. On Jun 14, 2011 this sequence version replaced NM_145687.2. Transcript Variant: This variant (3) lacks an alternate in-frame exon but includes a different in-frame exon in the central coding region, compared to variant 2. The encoded isoform (3) is shorter than isoform 2. The 5' UTR of this variant is incomplete due to a lack of 5'-complete transcripts representing this variant, and the presence of splicing ambiguity in the 5' UTR. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU794634.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC
    GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC
    AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA
    AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA
    GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC
    AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC
    GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT
    GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG
    ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT
    TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC
    AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT
    GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG
    ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT
    CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA
    GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA
    GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG
    GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT
    TATCTACCAA CACATATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT
    ACAGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT
    GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACATC AGTAGCATAT
    ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_663720.1
    CDS6..3644
    Translation

    Target ORF information:

    RefSeq Version NM_145687.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_145687.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC
    GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC
    AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA
    AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA
    GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC
    AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC
    GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT
    GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG
    ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT
    TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC
    AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT
    GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG
    ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT
    CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA
    GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA
    GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG
    GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT
    TATCTACCAA CACATATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT
    ACAGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT
    GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACATC AGTAGCATAT
    ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20375
    Clone ID Related Accession (Same CDS sequence) NM_145687.3 , NM_145687.4
    Accession Version NM_145687.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3639bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007005.3, DB086475.1, AB014587.1, AC005035.2 and BU625485.1. On Jun 2, 2019 this sequence version replaced NM_145687.3. Transcript Variant: This variant (3) lacks an alternate in-frame exon but includes a different in-frame exon in the central coding region, compared to variant 2. The encoded isoform (3) is shorter than isoform 2. The 5' UTR of this variant is incomplete due to a lack of 5'-complete transcripts representing this variant, and the presence of splicing ambiguity in the 5' UTR. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU794634.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC
    GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC
    AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA
    AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA
    GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC
    AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC
    GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT
    GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG
    ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT
    TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC
    AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT
    GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG
    ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT
    CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA
    GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA
    GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG
    GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT
    TATCTACCAA CACATATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT
    ACAGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT
    GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACATC AGTAGCATAT
    ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_663720.1
    CDS375..4013
    Translation

    Target ORF information:

    RefSeq Version NM_145687.4
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_145687.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC
    GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC
    AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA
    AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA
    GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC
    AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC
    GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT
    GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG
    ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT
    TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC
    AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT
    GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG
    ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT
    CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA
    GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA
    GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG
    GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT
    TATCTACCAA CACATATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT
    ACAGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT
    GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACATC AGTAGCATAT
    ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20734
    Clone ID Related Accession (Same CDS sequence) NM_145686.3
    Accession Version NM_145686.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002278.1, AC007005.3, BC064691.1, AC005035.2 and BU625485.1. On Jun 14, 2011 this sequence version replaced NM_145686.2. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064691.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC
    GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC
    AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA
    AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GATCTGACCG CACTGGCCAA AGAGCTTCGA
    GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG
    TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC
    TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA
    TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC
    AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT
    GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT
    TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG
    TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG
    ATTTCTCCAT CTAGCGGAAC AACAGTGACA TCTGTGGTGG GATTTTCCTG TGATGGGATG
    AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT
    ACCAACACTA GGCCACAGAG TGACACCCCG GAGATTCGTA AATACAAGAA GAGGTTTAAC
    TCTGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC
    CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTTATCAA CCGAAGACGA
    TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG
    GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA
    GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT
    AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAGAG TTCTGTGGAA
    GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA
    TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCAGGATC AGTCTATGAC
    ATTTATCTAC CAACACATAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC
    AATACAGATG GAATGGAGCT TCTGGTGTGC TATGAAGATG AGGGGGTTTA TGTAAACACA
    TATGGAAGGA TCACCAAGGA TGTAGTTCTA CAGTGGGGAG AGATGCCTAC ATCAGTAGCA
    TATATTCGAT CCAATCAGAC AATGGGCTGG GGAGAGAAGG CCATAGAGAT CCGATCTGTG
    GAAACTGGTC ACTTGGATGG TGTGTTCATG CACAAAAGGG CTCAAAGACT AAAATTCTTG
    TGTGAACGCA ATGACAAGGT GTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT
    TATTTCATGA CCTTAGGCAG GACTTCTCTT CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_663719.2
    CDS379..4200
    Translation

    Target ORF information:

    RefSeq Version NM_145686.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_145686.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC
    GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC
    AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA
    AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GATCTGACCG CACTGGCCAA AGAGCTTCGA
    GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG
    TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC
    TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA
    TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC
    AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT
    GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT
    TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG
    TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG
    ATTTCTCCAT CTAGCGGAAC AACAGTGACA TCTGTGGTGG GATTTTCCTG TGATGGGATG
    AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT
    ACCAACACTA GGCCACAGAG TGACACCCCG GAGATTCGTA AATACAAGAA GAGGTTTAAC
    TCTGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC
    CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTTATCAA CCGAAGACGA
    TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG
    GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA
    GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT
    AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAGAG TTCTGTGGAA
    GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA
    TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCAGGATC AGTCTATGAC
    ATTTATCTAC CAACACATAT CCAGTGTAGC ATCAAACCCC ATGCAATCAT CATCCTCCCC
    AATACAGATG GAATGGAGCT TCTGGTGTGC TATGAAGATG AGGGGGTTTA TGTAAACACA
    TATGGAAGGA TCACCAAGGA TGTAGTTCTA CAGTGGGGAG AGATGCCTAC ATCAGTAGCA
    TATATTCGAT CCAATCAGAC AATGGGCTGG GGAGAGAAGG CCATAGAGAT CCGATCTGTG
    GAAACTGGTC ACTTGGATGG TGTGTTCATG CACAAAAGGG CTCAAAGACT AAAATTCTTG
    TGTGAACGCA ATGACAAGGT GTTCTTTGCC TCTGTTCGGT CTGGTGGCAG CAGTCAGGTT
    TATTTCATGA CCTTAGGCAG GACTTCTCTT CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21057
    Clone ID Related Accession (Same CDS sequence) NM_004834.4 , NM_004834.5
    Accession Version NM_004834.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-09-29
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002278.1, AC007005.3, AF096300.1, AC005035.2 and BU625485.1. On Jun 14, 2011 this sequence version replaced NM_004834.3. Transcript Variant: This variant (1) lacks two alternate in-frame exons but includes a different in-frame exon in the central coding region, and uses an alternate in-frame splice site in both the central and 3' coding regions, compared to variant 2. The encoded isoform (1) is shorter than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF096300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGTA TTGAGCCCAG GCTTCTGTGG
    GAGAGAGTGG AGAAGCTGGT GCCCAGACCT GGCAGTGGCA GCTCCTCAGG GTCCAGCAAC
    TCAGGATCCC AGCCCGGGTC TCACCCTGGG TCTCAGAGTG GCTCCGGGGA ACGCTTCAGA
    GTGAGATCAT CATCCAAGTC TGAAGGCTCT CCATCTCAGC GCCTGGAAAA TGCAGTGAAA
    AAACCTGAAG ATAAAAAGGA AGTTTTCAGA CCCCTCAAGC CTGCTGGCGA AGTGGATCTG
    ACCGCACTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTAC GGCCACCTCA CAAAGTAACG
    GACTACTCCT CATCCAGTGA GGAGTCGGGG ACGACGGATG AGGAGGACGA CGATGTGGAG
    CAGGAAGGGG CTGACGAGTC CACCTCAGGA CCAGAGGACA CCAGAGCAGC GTCATCTCTG
    AATTTGAGCA ATGGTGAAAC GGAATCTGTG AAAACCATGA TTGTCCATGA TGATGTAGAA
    AGTGAGCCGG CCATGACCCC ATCCAAGGAG GGCACTCTAA TCGTCCGCCA GACTCAGTCC
    GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC
    AGATTACTAC AGATTTCTCC ATCTAGCGGA ACAACAGTGA CATCTGTGGT GGGATTTTCC
    TGTGATGGGA TGAGACCAGA AGCCATAAGG CAAGATCCTA CCCGGAAAGG CTCAGTGGTC
    AATGTGAATC CTACCAACAC TAGGCCACAG AGTGACACCC CGGAGATTCG TAAATACAAG
    AAGAGGTTTA ACTCTGAGAT TCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTGGGT
    ACAGAGAGTG GCCTGATGCT GCTGGACAGA AGTGGCCAAG GGAAGGTCTA TCCTCTTATC
    AACCGAAGAC GATTTCAACA AATGGACGTA CTTGAGGGCT TGAATGTCTT GGTGACAATA
    TCTGGCAAAA AGGATAAGTT ACGTGTCTAC TATTTGTCCT GGTTAAGAAA TAAAATACTT
    CACAATGATC CAGAAGTTGA GAAGAAGCAG GGATGGACAA CCGTAGGGGA TTTGGAAGGA
    TGTGTACATT ATAAAGTTGT AAAATATGAA AGAATCAAAT TTCTGGTGAT TGCTTTGAAG
    AGTTCTGTGG AAGTCTATGC GTGGGCACCA AAGCCATATC ACAAATTTAT GGCCTTTAAG
    TCATTTGGAG AATTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTTGA GGAAGGCCAG
    AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCAGGA
    TCAGTCTATG ACATTTATCT ACCAACACAT GTAAGAAAGA ACCCACACTC TATGATCCAG
    TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG
    GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA
    GTTCTACAGT GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG
    GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG
    TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC
    TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004825.3
    CDS379..3876
    Translation

    Target ORF information:

    RefSeq Version NM_004834.4
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004834.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGTA TTGAGCCCAG GCTTCTGTGG
    GAGAGAGTGG AGAAGCTGGT GCCCAGACCT GGCAGTGGCA GCTCCTCAGG GTCCAGCAAC
    TCAGGATCCC AGCCCGGGTC TCACCCTGGG TCTCAGAGTG GCTCCGGGGA ACGCTTCAGA
    GTGAGATCAT CATCCAAGTC TGAAGGCTCT CCATCTCAGC GCCTGGAAAA TGCAGTGAAA
    AAACCTGAAG ATAAAAAGGA AGTTTTCAGA CCCCTCAAGC CTGCTGGCGA AGTGGATCTG
    ACCGCACTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTAC GGCCACCTCA CAAAGTAACG
    GACTACTCCT CATCCAGTGA GGAGTCGGGG ACGACGGATG AGGAGGACGA CGATGTGGAG
    CAGGAAGGGG CTGACGAGTC CACCTCAGGA CCAGAGGACA CCAGAGCAGC GTCATCTCTG
    AATTTGAGCA ATGGTGAAAC GGAATCTGTG AAAACCATGA TTGTCCATGA TGATGTAGAA
    AGTGAGCCGG CCATGACCCC ATCCAAGGAG GGCACTCTAA TCGTCCGCCA GACTCAGTCC
    GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC
    AGATTACTAC AGATTTCTCC ATCTAGCGGA ACAACAGTGA CATCTGTGGT GGGATTTTCC
    TGTGATGGGA TGAGACCAGA AGCCATAAGG CAAGATCCTA CCCGGAAAGG CTCAGTGGTC
    AATGTGAATC CTACCAACAC TAGGCCACAG AGTGACACCC CGGAGATTCG TAAATACAAG
    AAGAGGTTTA ACTCTGAGAT TCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTGGGT
    ACAGAGAGTG GCCTGATGCT GCTGGACAGA AGTGGCCAAG GGAAGGTCTA TCCTCTTATC
    AACCGAAGAC GATTTCAACA AATGGACGTA CTTGAGGGCT TGAATGTCTT GGTGACAATA
    TCTGGCAAAA AGGATAAGTT ACGTGTCTAC TATTTGTCCT GGTTAAGAAA TAAAATACTT
    CACAATGATC CAGAAGTTGA GAAGAAGCAG GGATGGACAA CCGTAGGGGA TTTGGAAGGA
    TGTGTACATT ATAAAGTTGT AAAATATGAA AGAATCAAAT TTCTGGTGAT TGCTTTGAAG
    AGTTCTGTGG AAGTCTATGC GTGGGCACCA AAGCCATATC ACAAATTTAT GGCCTTTAAG
    TCATTTGGAG AATTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTTGA GGAAGGCCAG
    AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCAGGA
    TCAGTCTATG ACATTTATCT ACCAACACAT GTAAGAAAGA ACCCACACTC TATGATCCAG
    TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG
    GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA
    GTTCTACAGT GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG
    GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG
    TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC
    TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21057
    Clone ID Related Accession (Same CDS sequence) NM_004834.4 , NM_004834.5
    Accession Version NM_004834.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002278.1, AC007005.3, AF096300.1, AC005035.2 and BU625485.1. On Jun 2, 2019 this sequence version replaced NM_004834.4. Transcript Variant: This variant (1) lacks two alternate in-frame exons but includes a different in-frame exon in the central coding region, and uses an alternate in-frame splice site in both the central and 3' coding regions, compared to variant 2. The encoded isoform (1) is shorter than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF096300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGTA TTGAGCCCAG GCTTCTGTGG
    GAGAGAGTGG AGAAGCTGGT GCCCAGACCT GGCAGTGGCA GCTCCTCAGG GTCCAGCAAC
    TCAGGATCCC AGCCCGGGTC TCACCCTGGG TCTCAGAGTG GCTCCGGGGA ACGCTTCAGA
    GTGAGATCAT CATCCAAGTC TGAAGGCTCT CCATCTCAGC GCCTGGAAAA TGCAGTGAAA
    AAACCTGAAG ATAAAAAGGA AGTTTTCAGA CCCCTCAAGC CTGCTGGCGA AGTGGATCTG
    ACCGCACTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTAC GGCCACCTCA CAAAGTAACG
    GACTACTCCT CATCCAGTGA GGAGTCGGGG ACGACGGATG AGGAGGACGA CGATGTGGAG
    CAGGAAGGGG CTGACGAGTC CACCTCAGGA CCAGAGGACA CCAGAGCAGC GTCATCTCTG
    AATTTGAGCA ATGGTGAAAC GGAATCTGTG AAAACCATGA TTGTCCATGA TGATGTAGAA
    AGTGAGCCGG CCATGACCCC ATCCAAGGAG GGCACTCTAA TCGTCCGCCA GACTCAGTCC
    GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC
    AGATTACTAC AGATTTCTCC ATCTAGCGGA ACAACAGTGA CATCTGTGGT GGGATTTTCC
    TGTGATGGGA TGAGACCAGA AGCCATAAGG CAAGATCCTA CCCGGAAAGG CTCAGTGGTC
    AATGTGAATC CTACCAACAC TAGGCCACAG AGTGACACCC CGGAGATTCG TAAATACAAG
    AAGAGGTTTA ACTCTGAGAT TCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTGGGT
    ACAGAGAGTG GCCTGATGCT GCTGGACAGA AGTGGCCAAG GGAAGGTCTA TCCTCTTATC
    AACCGAAGAC GATTTCAACA AATGGACGTA CTTGAGGGCT TGAATGTCTT GGTGACAATA
    TCTGGCAAAA AGGATAAGTT ACGTGTCTAC TATTTGTCCT GGTTAAGAAA TAAAATACTT
    CACAATGATC CAGAAGTTGA GAAGAAGCAG GGATGGACAA CCGTAGGGGA TTTGGAAGGA
    TGTGTACATT ATAAAGTTGT AAAATATGAA AGAATCAAAT TTCTGGTGAT TGCTTTGAAG
    AGTTCTGTGG AAGTCTATGC GTGGGCACCA AAGCCATATC ACAAATTTAT GGCCTTTAAG
    TCATTTGGAG AATTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTTGA GGAAGGCCAG
    AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCAGGA
    TCAGTCTATG ACATTTATCT ACCAACACAT GTAAGAAAGA ACCCACACTC TATGATCCAG
    TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG
    GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA
    GTTCTACAGT GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG
    GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG
    TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC
    TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004825.3
    CDS375..3872
    Translation

    Target ORF information:

    RefSeq Version NM_004834.5
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004834.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGTA TTGAGCCCAG GCTTCTGTGG
    GAGAGAGTGG AGAAGCTGGT GCCCAGACCT GGCAGTGGCA GCTCCTCAGG GTCCAGCAAC
    TCAGGATCCC AGCCCGGGTC TCACCCTGGG TCTCAGAGTG GCTCCGGGGA ACGCTTCAGA
    GTGAGATCAT CATCCAAGTC TGAAGGCTCT CCATCTCAGC GCCTGGAAAA TGCAGTGAAA
    AAACCTGAAG ATAAAAAGGA AGTTTTCAGA CCCCTCAAGC CTGCTGGCGA AGTGGATCTG
    ACCGCACTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTAC GGCCACCTCA CAAAGTAACG
    GACTACTCCT CATCCAGTGA GGAGTCGGGG ACGACGGATG AGGAGGACGA CGATGTGGAG
    CAGGAAGGGG CTGACGAGTC CACCTCAGGA CCAGAGGACA CCAGAGCAGC GTCATCTCTG
    AATTTGAGCA ATGGTGAAAC GGAATCTGTG AAAACCATGA TTGTCCATGA TGATGTAGAA
    AGTGAGCCGG CCATGACCCC ATCCAAGGAG GGCACTCTAA TCGTCCGCCA GACTCAGTCC
    GCTAGTAGCA CACTCCAGAA ACACAAATCT TCCTCCTCCT TTACACCTTT TATAGACCCC
    AGATTACTAC AGATTTCTCC ATCTAGCGGA ACAACAGTGA CATCTGTGGT GGGATTTTCC
    TGTGATGGGA TGAGACCAGA AGCCATAAGG CAAGATCCTA CCCGGAAAGG CTCAGTGGTC
    AATGTGAATC CTACCAACAC TAGGCCACAG AGTGACACCC CGGAGATTCG TAAATACAAG
    AAGAGGTTTA ACTCTGAGAT TCTGTGTGCT GCCTTATGGG GAGTGAATTT GCTAGTGGGT
    ACAGAGAGTG GCCTGATGCT GCTGGACAGA AGTGGCCAAG GGAAGGTCTA TCCTCTTATC
    AACCGAAGAC GATTTCAACA AATGGACGTA CTTGAGGGCT TGAATGTCTT GGTGACAATA
    TCTGGCAAAA AGGATAAGTT ACGTGTCTAC TATTTGTCCT GGTTAAGAAA TAAAATACTT
    CACAATGATC CAGAAGTTGA GAAGAAGCAG GGATGGACAA CCGTAGGGGA TTTGGAAGGA
    TGTGTACATT ATAAAGTTGT AAAATATGAA AGAATCAAAT TTCTGGTGAT TGCTTTGAAG
    AGTTCTGTGG AAGTCTATGC GTGGGCACCA AAGCCATATC ACAAATTTAT GGCCTTTAAG
    TCATTTGGAG AATTGGTACA TAAGCCATTA CTGGTGGATC TCACTGTTGA GGAAGGCCAG
    AGGTTGAAAG TGATCTATGG ATCCTGTGCT GGATTCCATG CTGTTGATGT GGATTCAGGA
    TCAGTCTATG ACATTTATCT ACCAACACAT GTAAGAAAGA ACCCACACTC TATGATCCAG
    TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG
    GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA
    GTTCTACAGT GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG
    GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG
    TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC
    TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21579
    Clone ID Related Accession (Same CDS sequence) NM_001242560.2 , NM_001242560.1
    Accession Version NM_001242560.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002278.1, AC007005.3, AK296396.1, AC005035.2 and BU625485.1. On Jun 2, 2019 this sequence version replaced NM_001242560.1. Transcript Variant: This variant (5) lacks two alternate in-frame exons but includes two different alternate in-frame exons in the central coding region, compared to variant 2. The encoded isoform (5) is shorter than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296396.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC
    CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA
    TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT
    AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA
    GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA
    TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA
    TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT
    GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA
    CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC
    TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA
    GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG
    AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG
    CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA
    CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT
    GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG
    AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG
    TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA
    AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT
    GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA
    CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC
    TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA AACCCCATGC AATCATCATC
    CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA
    AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA
    GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA
    TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA
    TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT
    CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001229489.1
    CDS375..4082
    Translation

    Target ORF information:

    RefSeq Version NM_001242560.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001242560.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC
    CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA
    TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT
    AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA
    GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA
    TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA
    TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT
    GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA
    CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC
    TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA
    GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG
    AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG
    CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA
    CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT
    GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG
    AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG
    TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA
    AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT
    GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA
    CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC
    TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA AACCCCATGC AATCATCATC
    CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA
    AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA
    GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA
    TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA
    TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT
    CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu21579
    Clone ID Related Accession (Same CDS sequence) NM_001242560.2 , NM_001242560.1
    Accession Version NM_001242560.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-09-29
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002278.1, AC007005.3, AK296396.1, AC005035.2 and BU625485.1. Transcript Variant: This variant (5) lacks two alternate in-frame exons but includes two different alternate in-frame exons in the central coding region, compared to variant 2. The encoded isoform (5) is shorter than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296396.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC
    CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA
    TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT
    AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA
    GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA
    TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA
    TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT
    GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA
    CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC
    TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA
    GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG
    AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG
    CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA
    CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT
    GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG
    AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG
    TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA
    AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT
    GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA
    CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC
    TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA AACCCCATGC AATCATCATC
    CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA
    AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA
    GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA
    TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA
    TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT
    CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001229489.1
    CDS379..4086
    Translation

    Target ORF information:

    RefSeq Version NM_001242560.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001242560.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC
    CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA
    TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT
    AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA
    GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA
    TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA
    TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT
    GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA
    CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC
    TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA
    GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG
    AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG
    CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGACTCA GTCCGCTAGT
    AGCACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA
    CTACAGATTT CTCCATCTAG CGGAACAACA GTGACATCTG TGGTGGGATT TTCCTGTGAT
    GGGATGAGAC CAGAAGCCAT AAGGCAAGAT CCTACCCGGA AAGGCTCAGT GGTCAATGTG
    AATCCTACCA ACACTAGGCC ACAGAGTGAC ACCCCGGAGA TTCGTAAATA CAAGAAGAGG
    TTTAACTCTG AGATTCTGTG TGCTGCCTTA TGGGGAGTGA ATTTGCTAGT GGGTACAGAG
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TCTATCCTCT TATCAACCGA
    AGACGATTTC AACAAATGGA CGTACTTGAG GGCTTGAATG TCTTGGTGAC AATATCTGGC
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAT
    GATCCAGAAG TTGAGAAGAA GCAGGGATGG ACAACCGTAG GGGATTTGGA AGGATGTGTA
    CATTATAAAG TTGTAAAATA TGAAAGAATC AAATTTCTGG TGATTGCTTT GAAGAGTTCT
    GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA TATCACAAAT TTATGGCCTT TAAGTCATTT
    GGAGAATTGG TACATAAGCC ATTACTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGTTG
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGATTC AGGATCAGTC
    TATGACATTT ATCTACCAAC ACATATCCAG TGTAGCATCA AACCCCATGC AATCATCATC
    CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA
    AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA
    GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA
    TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA
    TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT
    CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22392
    Clone ID Related Accession (Same CDS sequence) NM_001242559.1 , NM_001242559.2
    Accession Version NM_001242559.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB086475.1, AY212247.1, AC005035.2 and BU625485.1. Transcript Variant: This variant (4) uses two alternate in-frame splice sites and lacks an alternate in-frame exon in the central coding region, compared to variant 2. The encoded isoform (4) is shorter than isoform 2. The 5' UTR of this variant is incomplete due to a lack of 5'-complete transcripts representing this variant, and the presence of splicing ambiguity in the 5' UTR. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY212247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA
    CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG
    CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC
    TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC
    GGGGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG
    GAAAATGCAG TGAAAAAACC TGAAGATAAA AAGGAAGTTT TCAGACCCCT CAAGCCTGCT
    GATCTGACCG CACTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTACGGCC ACCTCACAAA
    GTAACGGACT ACTCCTCATC CAGTGAGGAG TCGGGGACGA CGGATGAGGA GGACGACGAT
    GTGGAGCAGG AAGGGGCTGA CGAGTCCACC TCAGGACCAG AGGACACCAG AGCAGCGTCA
    TCTCTGAATT TGAGCAATGG TGAAACGGAA TCTGTGAAAA CCATGATTGT CCATGATGAT
    GTAGAAAGTG AGCCGGCCAT GACCCCATCC AAGGAGGGCA CTCTAATCGT CCGCCAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA
    TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA
    GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA
    TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA
    GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT
    CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA
    ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG
    GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT
    TCAGGATCAG TCTATGACAT TTATCTACCA ACACATATCC AGTGTAGCAT CAAACCCCAT
    GCAATCATCA TCCTCCCCAA TACAGATGGA ATGGAGCTTC TGGTGTGCTA TGAAGATGAG
    GGGGTTTATG TAAACACATA TGGAAGGATC ACCAAGGATG TAGTTCTACA GTGGGGAGAG
    ATGCCTACAT CAGTAGCATA TATTCGATCC AATCAGACAA TGGGCTGGGG AGAGAAGGCC
    ATAGAGATCC GATCTGTGGA AACTGGTCAC TTGGATGGTG TGTTCATGCA CAAAAGGGCT
    CAAAGACTAA AATTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC TGTTCGGTCT
    GGTGGCAGCA GTCAGGTTTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001229488.1
    CDS6..3725
    Translation

    Target ORF information:

    RefSeq Version NM_001242559.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001242559.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA
    CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG
    CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC
    TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC
    GGGGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG
    GAAAATGCAG TGAAAAAACC TGAAGATAAA AAGGAAGTTT TCAGACCCCT CAAGCCTGCT
    GATCTGACCG CACTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTACGGCC ACCTCACAAA
    GTAACGGACT ACTCCTCATC CAGTGAGGAG TCGGGGACGA CGGATGAGGA GGACGACGAT
    GTGGAGCAGG AAGGGGCTGA CGAGTCCACC TCAGGACCAG AGGACACCAG AGCAGCGTCA
    TCTCTGAATT TGAGCAATGG TGAAACGGAA TCTGTGAAAA CCATGATTGT CCATGATGAT
    GTAGAAAGTG AGCCGGCCAT GACCCCATCC AAGGAGGGCA CTCTAATCGT CCGCCAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA
    TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA
    GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA
    TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA
    GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT
    CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA
    ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG
    GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT
    TCAGGATCAG TCTATGACAT TTATCTACCA ACACATATCC AGTGTAGCAT CAAACCCCAT
    GCAATCATCA TCCTCCCCAA TACAGATGGA ATGGAGCTTC TGGTGTGCTA TGAAGATGAG
    GGGGTTTATG TAAACACATA TGGAAGGATC ACCAAGGATG TAGTTCTACA GTGGGGAGAG
    ATGCCTACAT CAGTAGCATA TATTCGATCC AATCAGACAA TGGGCTGGGG AGAGAAGGCC
    ATAGAGATCC GATCTGTGGA AACTGGTCAC TTGGATGGTG TGTTCATGCA CAAAAGGGCT
    CAAAGACTAA AATTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC TGTTCGGTCT
    GGTGGCAGCA GTCAGGTTTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22392
    Clone ID Related Accession (Same CDS sequence) NM_001242559.1 , NM_001242559.2
    Accession Version NM_001242559.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007005.3, DB086475.1, AY212247.1, AC005035.2 and BU625485.1. On Jun 2, 2019 this sequence version replaced NM_001242559.1. Transcript Variant: This variant (4) uses two alternate in-frame splice sites and lacks an alternate in-frame exon in the central coding region, compared to variant 2. The encoded isoform (4) is shorter than isoform 2. The 5' UTR of this variant is incomplete due to a lack of 5'-complete transcripts representing this variant, and the presence of splicing ambiguity in the 5' UTR. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY212247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA
    CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG
    CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC
    TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC
    GGGGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG
    GAAAATGCAG TGAAAAAACC TGAAGATAAA AAGGAAGTTT TCAGACCCCT CAAGCCTGCT
    GATCTGACCG CACTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTACGGCC ACCTCACAAA
    GTAACGGACT ACTCCTCATC CAGTGAGGAG TCGGGGACGA CGGATGAGGA GGACGACGAT
    GTGGAGCAGG AAGGGGCTGA CGAGTCCACC TCAGGACCAG AGGACACCAG AGCAGCGTCA
    TCTCTGAATT TGAGCAATGG TGAAACGGAA TCTGTGAAAA CCATGATTGT CCATGATGAT
    GTAGAAAGTG AGCCGGCCAT GACCCCATCC AAGGAGGGCA CTCTAATCGT CCGCCAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA
    TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA
    GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA
    TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA
    GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT
    CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA
    ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG
    GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT
    TCAGGATCAG TCTATGACAT TTATCTACCA ACACATATCC AGTGTAGCAT CAAACCCCAT
    GCAATCATCA TCCTCCCCAA TACAGATGGA ATGGAGCTTC TGGTGTGCTA TGAAGATGAG
    GGGGTTTATG TAAACACATA TGGAAGGATC ACCAAGGATG TAGTTCTACA GTGGGGAGAG
    ATGCCTACAT CAGTAGCATA TATTCGATCC AATCAGACAA TGGGCTGGGG AGAGAAGGCC
    ATAGAGATCC GATCTGTGGA AACTGGTCAC TTGGATGGTG TGTTCATGCA CAAAAGGGCT
    CAAAGACTAA AATTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC TGTTCGGTCT
    GGTGGCAGCA GTCAGGTTTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001229488.1
    CDS375..4094
    Translation

    Target ORF information:

    RefSeq Version NM_001242559.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001242559.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA
    CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGTATTGAG
    CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG CTGGTGCCCA GACCTGGCAG TGGCAGCTCC
    TCAGGGTCCA GCAACTCAGG ATCCCAGCCC GGGTCTCACC CTGGGTCTCA GAGTGGCTCC
    GGGGAACGCT TCAGAGTGAG ATCATCATCC AAGTCTGAAG GCTCTCCATC TCAGCGCCTG
    GAAAATGCAG TGAAAAAACC TGAAGATAAA AAGGAAGTTT TCAGACCCCT CAAGCCTGCT
    GATCTGACCG CACTGGCCAA AGAGCTTCGA GCAGTGGAAG ATGTACGGCC ACCTCACAAA
    GTAACGGACT ACTCCTCATC CAGTGAGGAG TCGGGGACGA CGGATGAGGA GGACGACGAT
    GTGGAGCAGG AAGGGGCTGA CGAGTCCACC TCAGGACCAG AGGACACCAG AGCAGCGTCA
    TCTCTGAATT TGAGCAATGG TGAAACGGAA TCTGTGAAAA CCATGATTGT CCATGATGAT
    GTAGAAAGTG AGCCGGCCAT GACCCCATCC AAGGAGGGCA CTCTAATCGT CCGCCAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA
    TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA
    GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA
    TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA
    GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT
    CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA
    ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG
    GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT
    TCAGGATCAG TCTATGACAT TTATCTACCA ACACATATCC AGTGTAGCAT CAAACCCCAT
    GCAATCATCA TCCTCCCCAA TACAGATGGA ATGGAGCTTC TGGTGTGCTA TGAAGATGAG
    GGGGTTTATG TAAACACATA TGGAAGGATC ACCAAGGATG TAGTTCTACA GTGGGGAGAG
    ATGCCTACAT CAGTAGCATA TATTCGATCC AATCAGACAA TGGGCTGGGG AGAGAAGGCC
    ATAGAGATCC GATCTGTGGA AACTGGTCAC TTGGATGGTG TGTTCATGCA CAAAAGGGCT
    CAAAGACTAA AATTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC TGTTCGGTCT
    GGTGGCAGCA GTCAGGTTTA TTTCATGACC TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36834
    Clone ID Related Accession (Same CDS sequence) XM_005264057.3
    Accession Version XM_005264057.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3948bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264057.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA
    CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT
    GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC
    TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC
    TCCGGGGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC
    CTGGAAAATG CAGTGAAAAA ACCTGAAGAT AAAAAGGAAG TTTTCAGACC CCTCAAGCCT
    GCTGGCGAAG TGGATCTGAC CGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTACGG
    CCACCTCACA AAGTAACGGA CTACTCCTCA TCCAGTGAGG AGTCGGGGAC GACGGATGAG
    GAGGACGACG ATGTGGAGCA GGAAGGGGCT GACGAGTCCA CCTCAGGACC AGAGGACACC
    AGAGCAGCGT CATCTCTGAA TTTGAGCAAT GGTGAAACGG AATCTGTGAA AACCATGATT
    GTCCATGATG ATGTAGAAAG TGAGCCGGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC
    GTCCGCCAGA GTACAGTTGA CCAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCC
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC
    ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG
    GACAAGCTCA CTGCTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGCGGA
    ACAACAGTGA CATCTGTGGT GGGATTTTCC TGTGATGGGA TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CCCGGAAAGG CTCAGTGGTC AATGTGAATC CTACCAACAC TAGGCCACAG
    AGTGACACCC CGGAGATTCG TAAATACAAG AAGAGGTTTA ACTCTGAGAT TCTGTGTGCT
    GCCTTATGGG GAGTGAATTT GCTAGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA
    AGTGGCCAAG GGAAGGTCTA TCCTCTTATC AACCGAAGAC GATTTCAACA AATGGACGTA
    CTTGAGGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC
    TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAATGATC CAGAAGTTGA GAAGAAGCAG
    GGATGGACAA CCGTAGGGGA TTTGGAAGGA TGTGTACATT ATAAAGTTGT AAAATATGAA
    AGAATCAAAT TTCTGGTGAT TGCTTTGAAG AGTTCTGTGG AAGTCTATGC GTGGGCACCA
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AATTGGTACA TAAGCCATTA
    CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT
    GGATTCCATG CTGTTGATGT GGATTCAGGA TCAGTCTATG ACATTTATCT ACCAACACAT
    GTAAGAAAGA ACCCACACTC TATGATCCAG TGTAGCATCA AACCCCATGC AATCATCATC
    CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA
    AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA
    GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA
    TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA
    TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT
    CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264114.1
    CDS75..4022
    Translation

    Target ORF information:

    RefSeq Version XM_005264057.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264057.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA
    CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT
    GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC
    TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC
    TCCGGGGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC
    CTGGAAAATG CAGTGAAAAA ACCTGAAGAT AAAAAGGAAG TTTTCAGACC CCTCAAGCCT
    GCTGGCGAAG TGGATCTGAC CGCACTGGCC AAAGAGCTTC GAGCAGTGGA AGATGTACGG
    CCACCTCACA AAGTAACGGA CTACTCCTCA TCCAGTGAGG AGTCGGGGAC GACGGATGAG
    GAGGACGACG ATGTGGAGCA GGAAGGGGCT GACGAGTCCA CCTCAGGACC AGAGGACACC
    AGAGCAGCGT CATCTCTGAA TTTGAGCAAT GGTGAAACGG AATCTGTGAA AACCATGATT
    GTCCATGATG ATGTAGAAAG TGAGCCGGCC ATGACCCCAT CCAAGGAGGG CACTCTAATC
    GTCCGCCAGA GTACAGTTGA CCAAAAGCGT GCCAGCCATC ATGAGAGCAA TGGCTTTGCC
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC
    ACCTCCTCCT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG
    GACAAGCTCA CTGCTAATGA GACTCAGTCC GCTAGTAGCA CACTCCAGAA ACACAAATCT
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGCGGA
    ACAACAGTGA CATCTGTGGT GGGATTTTCC TGTGATGGGA TGAGACCAGA AGCCATAAGG
    CAAGATCCTA CCCGGAAAGG CTCAGTGGTC AATGTGAATC CTACCAACAC TAGGCCACAG
    AGTGACACCC CGGAGATTCG TAAATACAAG AAGAGGTTTA ACTCTGAGAT TCTGTGTGCT
    GCCTTATGGG GAGTGAATTT GCTAGTGGGT ACAGAGAGTG GCCTGATGCT GCTGGACAGA
    AGTGGCCAAG GGAAGGTCTA TCCTCTTATC AACCGAAGAC GATTTCAACA AATGGACGTA
    CTTGAGGGCT TGAATGTCTT GGTGACAATA TCTGGCAAAA AGGATAAGTT ACGTGTCTAC
    TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAATGATC CAGAAGTTGA GAAGAAGCAG
    GGATGGACAA CCGTAGGGGA TTTGGAAGGA TGTGTACATT ATAAAGTTGT AAAATATGAA
    AGAATCAAAT TTCTGGTGAT TGCTTTGAAG AGTTCTGTGG AAGTCTATGC GTGGGCACCA
    AAGCCATATC ACAAATTTAT GGCCTTTAAG TCATTTGGAG AATTGGTACA TAAGCCATTA
    CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGTTGAAAG TGATCTATGG ATCCTGTGCT
    GGATTCCATG CTGTTGATGT GGATTCAGGA TCAGTCTATG ACATTTATCT ACCAACACAT
    GTAAGAAAGA ACCCACACTC TATGATCCAG TGTAGCATCA AACCCCATGC AATCATCATC
    CTCCCCAATA CAGATGGAAT GGAGCTTCTG GTGTGCTATG AAGATGAGGG GGTTTATGTA
    AACACATATG GAAGGATCAC CAAGGATGTA GTTCTACAGT GGGGAGAGAT GCCTACATCA
    GTAGCATATA TTCGATCCAA TCAGACAATG GGCTGGGGAG AGAAGGCCAT AGAGATCCGA
    TCTGTGGAAA CTGGTCACTT GGATGGTGTG TTCATGCACA AAAGGGCTCA AAGACTAAAA
    TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT
    CAGGTTTATT TCATGACCTT AGGCAGGACT TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36835
    Clone ID Related Accession (Same CDS sequence) XM_005264058.3
    Accession Version XM_005264058.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3939bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264058.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA
    CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT
    GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC
    TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC
    TCCGGGGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC
    CTGGAAAATG CAGTGAAAAA ACCTGAAGAT AAAAAGGAAG TTTTCAGACC CCTCAAGCCT
    GCTGATCTGA CCGCACTGGC CAAAGAGCTT CGAGCAGTGG AAGATGTACG GCCACCTCAC
    AAAGTAACGG ACTACTCCTC ATCCAGTGAG GAGTCGGGGA CGACGGATGA GGAGGACGAC
    GATGTGGAGC AGGAAGGGGC TGACGAGTCC ACCTCAGGAC CAGAGGACAC CAGAGCAGCG
    TCATCTCTGA ATTTGAGCAA TGGTGAAACG GAATCTGTGA AAACCATGAT TGTCCATGAT
    GATGTAGAAA GTGAGCCGGC CATGACCCCA TCCAAGGAGG GCACTCTAAT CGTCCGCCAG
    AGTACAGTTG ACCAAAAGCG TGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT
    CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC
    TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC
    ACTGCTAATG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC
    TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGCGG AACAACAGTG
    ACATCTGTGG TGGGATTTTC CTGTGATGGG ATGAGACCAG AAGCCATAAG GCAAGATCCT
    ACCCGGAAAG GCTCAGTGGT CAATGTGAAT CCTACCAACA CTAGGCCACA GAGTGACACC
    CCGGAGATTC GTAAATACAA GAAGAGGTTT AACTCTGAGA TTCTGTGTGC TGCCTTATGG
    GGAGTGAATT TGCTAGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA
    GGGAAGGTCT ATCCTCTTAT CAACCGAAGA CGATTTCAAC AAATGGACGT ACTTGAGGGC
    TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC
    TGGTTAAGAA ATAAAATACT TCACAATGAT CCAGAAGTTG AGAAGAAGCA GGGATGGACA
    ACCGTAGGGG ATTTGGAAGG ATGTGTACAT TATAAAGTTG TAAAATATGA AAGAATCAAA
    TTTCTGGTGA TTGCTTTGAA GAGTTCTGTG GAAGTCTATG CGTGGGCACC AAAGCCATAT
    CACAAATTTA TGGCCTTTAA GTCATTTGGA GAATTGGTAC ATAAGCCATT ACTGGTGGAT
    CTCACTGTTG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT
    GCTGTTGATG TGGATTCAGG ATCAGTCTAT GACATTTATC TACCAACACA TGTAAGAAAG
    AACCCACACT CTATGATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT
    ACAGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT
    GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACATC AGTAGCATAT
    ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264115.1
    CDS75..4013
    Translation

    Target ORF information:

    RefSeq Version XM_005264058.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264058.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTTC CTGTGAGAAC AACATCTCGC TCCCCTGTTC TGTCCCGTCG AGATTCCCCA
    CTGCAGGGCA GTGGGCAGCA GAATAGCCAG GCAGGACAGA GAAACTCCAC CAGCAGTATT
    GAGCCCAGGC TTCTGTGGGA GAGAGTGGAG AAGCTGGTGC CCAGACCTGG CAGTGGCAGC
    TCCTCAGGGT CCAGCAACTC AGGATCCCAG CCCGGGTCTC ACCCTGGGTC TCAGAGTGGC
    TCCGGGGAAC GCTTCAGAGT GAGATCATCA TCCAAGTCTG AAGGCTCTCC ATCTCAGCGC
    CTGGAAAATG CAGTGAAAAA ACCTGAAGAT AAAAAGGAAG TTTTCAGACC CCTCAAGCCT
    GCTGATCTGA CCGCACTGGC CAAAGAGCTT CGAGCAGTGG AAGATGTACG GCCACCTCAC
    AAAGTAACGG ACTACTCCTC ATCCAGTGAG GAGTCGGGGA CGACGGATGA GGAGGACGAC
    GATGTGGAGC AGGAAGGGGC TGACGAGTCC ACCTCAGGAC CAGAGGACAC CAGAGCAGCG
    TCATCTCTGA ATTTGAGCAA TGGTGAAACG GAATCTGTGA AAACCATGAT TGTCCATGAT
    GATGTAGAAA GTGAGCCGGC CATGACCCCA TCCAAGGAGG GCACTCTAAT CGTCCGCCAG
    AGTACAGTTG ACCAAAAGCG TGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT
    CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC
    TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC
    ACTGCTAATG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC
    TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGCGG AACAACAGTG
    ACATCTGTGG TGGGATTTTC CTGTGATGGG ATGAGACCAG AAGCCATAAG GCAAGATCCT
    ACCCGGAAAG GCTCAGTGGT CAATGTGAAT CCTACCAACA CTAGGCCACA GAGTGACACC
    CCGGAGATTC GTAAATACAA GAAGAGGTTT AACTCTGAGA TTCTGTGTGC TGCCTTATGG
    GGAGTGAATT TGCTAGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA
    GGGAAGGTCT ATCCTCTTAT CAACCGAAGA CGATTTCAAC AAATGGACGT ACTTGAGGGC
    TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC
    TGGTTAAGAA ATAAAATACT TCACAATGAT CCAGAAGTTG AGAAGAAGCA GGGATGGACA
    ACCGTAGGGG ATTTGGAAGG ATGTGTACAT TATAAAGTTG TAAAATATGA AAGAATCAAA
    TTTCTGGTGA TTGCTTTGAA GAGTTCTGTG GAAGTCTATG CGTGGGCACC AAAGCCATAT
    CACAAATTTA TGGCCTTTAA GTCATTTGGA GAATTGGTAC ATAAGCCATT ACTGGTGGAT
    CTCACTGTTG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT
    GCTGTTGATG TGGATTCAGG ATCAGTCTAT GACATTTATC TACCAACACA TGTAAGAAAG
    AACCCACACT CTATGATCCA GTGTAGCATC AAACCCCATG CAATCATCAT CCTCCCCAAT
    ACAGATGGAA TGGAGCTTCT GGTGTGCTAT GAAGATGAGG GGGTTTATGT AAACACATAT
    GGAAGGATCA CCAAGGATGT AGTTCTACAG TGGGGAGAGA TGCCTACATC AGTAGCATAT
    ATTCGATCCA ATCAGACAAT GGGCTGGGGA GAGAAGGCCA TAGAGATCCG ATCTGTGGAA
    ACTGGTCACT TGGATGGTGT GTTCATGCAC AAAAGGGCTC AAAGACTAAA ATTCTTGTGT
    GAACGCAATG ACAAGGTGTT CTTTGCCTCT GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT
    TTCATGACCT TAGGCAGGAC TTCTCTTCTG AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36836
    Clone ID Related Accession (Same CDS sequence) XM_006712865.2
    Accession Version XM_006712865.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006712865.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC
    CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA
    TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT
    AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA
    GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA
    TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA
    TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT
    GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA
    CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC
    TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA
    GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG
    AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG
    CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGAGTAC AGTTGACCAA
    AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCCGGTC GCATTCACCT CTTGCCAGAT
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCCTCCCC ATCCTCCAGC
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA
    TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA
    GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA
    TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA
    GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT
    CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA
    ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG
    GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT
    TCAGGATCAG TCTATGACAT TTATCTACCA ACACATGTAA GAAAGAACCC ACACTCTATG
    ATCCAGTGTA GCATCAAACC CCATGCAATC ATCATCCTCC CCAATACAGA TGGAATGGAG
    CTTCTGGTGT GCTATGAAGA TGAGGGGGTT TATGTAAACA CATATGGAAG GATCACCAAG
    GATGTAGTTC TACAGTGGGG AGAGATGCCT ACATCAGTAG CATATATTCG ATCCAATCAG
    ACAATGGGCT GGGGAGAGAA GGCCATAGAG ATCCGATCTG TGGAAACTGG TCACTTGGAT
    GGTGTGTTCA TGCACAAAAG GGCTCAAAGA CTAAAATTCT TGTGTGAACG CAATGACAAG
    GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC AGCAGTCAGG TTTATTTCAT GACCTTAGGC
    AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712928.1
    CDS75..3998
    Translation

    Target ORF information:

    RefSeq Version XM_006712865.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712865.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTACAGTGG
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGCGATCCCA TTCCTTCAGT
    GACCCTTCTC CCAAATTTGC ACACCACCAT CTTCGTTCTC AGGACCCATG TCCACCTTCC
    CGCAGTGAGG TGCTCAGTCA GAGCTCTGAC TCTAAGTCAG AGGCGCCTGA CCCTACCCAA
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTTCCTGT GAGAACAACA
    TCTCGCTCCC CTGTTCTGTC CCGTCGAGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAAT
    AGCCAGGCAG GACAGAGAAA CTCCACCAGC AGTATTGAGC CCAGGCTTCT GTGGGAGAGA
    GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT GGCAGCTCCT CAGGGTCCAG CAACTCAGGA
    TCCCAGCCCG GGTCTCACCC TGGGTCTCAG AGTGGCTCCG GGGAACGCTT CAGAGTGAGA
    TCATCATCCA AGTCTGAAGG CTCTCCATCT CAGCGCCTGG AAAATGCAGT GAAAAAACCT
    GAAGATAAAA AGGAAGTTTT CAGACCCCTC AAGCCTGCTG GCGAAGTGGA TCTGACCGCA
    CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC
    TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA
    GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG
    AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG
    CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGAGTAC AGTTGACCAA
    AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCCGGTC GCATTCACCT CTTGCCAGAT
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCCTCCCC ATCCTCCAGC
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA
    TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA
    GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA
    TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA
    GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT
    CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA
    ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG
    GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT
    TCAGGATCAG TCTATGACAT TTATCTACCA ACACATGTAA GAAAGAACCC ACACTCTATG
    ATCCAGTGTA GCATCAAACC CCATGCAATC ATCATCCTCC CCAATACAGA TGGAATGGAG
    CTTCTGGTGT GCTATGAAGA TGAGGGGGTT TATGTAAACA CATATGGAAG GATCACCAAG
    GATGTAGTTC TACAGTGGGG AGAGATGCCT ACATCAGTAG CATATATTCG ATCCAATCAG
    ACAATGGGCT GGGGAGAGAA GGCCATAGAG ATCCGATCTG TGGAAACTGG TCACTTGGAT
    GGTGTGTTCA TGCACAAAAG GGCTCAAAGA CTAAAATTCT TGTGTGAACG CAATGACAAG
    GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC AGCAGTCAGG TTTATTTCAT GACCTTAGGC
    AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36838
    Clone ID Related Accession (Same CDS sequence) XM_005264059.3
    Accession Version XM_005264059.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3855bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264059.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC
    GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC
    AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA
    AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA
    GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC
    AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC
    GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT
    GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG
    ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGAGTA CAGTTGACCA AAAGCGTGCC
    AGCCATCATG AGAGCAATGG CTTTGCCGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG
    CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCCTCCC CATCCTCCAG CCAGCCGACA
    CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTG CTAATGAGAC TCAGTCCGCT
    AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA
    TTACTACAGA TTTCTCCATC TAGCGGAACA ACAGTGACAT CTGTGGTGGG ATTTTCCTGT
    GATGGGATGA GACCAGAAGC CATAAGGCAA GATCCTACCC GGAAAGGCTC AGTGGTCAAT
    GTGAATCCTA CCAACACTAG GCCACAGAGT GACACCCCGG AGATTCGTAA ATACAAGAAG
    AGGTTTAACT CTGAGATTCT GTGTGCTGCC TTATGGGGAG TGAATTTGCT AGTGGGTACA
    GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTCTATCC TCTTATCAAC
    CGAAGACGAT TTCAACAAAT GGACGTACTT GAGGGCTTGA ATGTCTTGGT GACAATATCT
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC
    AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACAACCG TAGGGGATTT GGAAGGATGT
    GTACATTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC TGGTGATTGC TTTGAAGAGT
    TCTGTGGAAG TCTATGCGTG GGCACCAAAG CCATATCACA AATTTATGGC CTTTAAGTCA
    TTTGGAGAAT TGGTACATAA GCCATTACTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG
    TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA TTCAGGATCA
    GTCTATGACA TTTATCTACC AACACATGTA AGAAAGAACC CACACTCTAT GATCCAGTGT
    AGCATCAAAC CCCATGCAAT CATCATCCTC CCCAATACAG ATGGAATGGA GCTTCTGGTG
    TGCTATGAAG ATGAGGGGGT TTATGTAAAC ACATATGGAA GGATCACCAA GGATGTAGTT
    CTACAGTGGG GAGAGATGCC TACATCAGTA GCATATATTC GATCCAATCA GACAATGGGC
    TGGGGAGAGA AGGCCATAGA GATCCGATCT GTGGAAACTG GTCACTTGGA TGGTGTGTTC
    ATGCACAAAA GGGCTCAAAG ACTAAAATTC TTGTGTGAAC GCAATGACAA GGTGTTCTTT
    GCCTCTGTTC GGTCTGGTGG CAGCAGTCAG GTTTATTTCA TGACCTTAGG CAGGACTTCT
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264116.1
    CDS75..3929
    Translation

    Target ORF information:

    RefSeq Version XM_005264059.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264059.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC
    GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC
    AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA
    AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GGCGAAGTGG ATCTGACCGC ACTGGCCAAA
    GAGCTTCGAG CAGTGGAAGA TGTACGGCCA CCTCACAAAG TAACGGACTA CTCCTCATCC
    AGTGAGGAGT CGGGGACGAC GGATGAGGAG GACGACGATG TGGAGCAGGA AGGGGCTGAC
    GAGTCCACCT CAGGACCAGA GGACACCAGA GCAGCGTCAT CTCTGAATTT GAGCAATGGT
    GAAACGGAAT CTGTGAAAAC CATGATTGTC CATGATGATG TAGAAAGTGA GCCGGCCATG
    ACCCCATCCA AGGAGGGCAC TCTAATCGTC CGCCAGAGTA CAGTTGACCA AAAGCGTGCC
    AGCCATCATG AGAGCAATGG CTTTGCCGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG
    CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCCTCCC CATCCTCCAG CCAGCCGACA
    CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTG CTAATGAGAC TCAGTCCGCT
    AGTAGCACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA
    TTACTACAGA TTTCTCCATC TAGCGGAACA ACAGTGACAT CTGTGGTGGG ATTTTCCTGT
    GATGGGATGA GACCAGAAGC CATAAGGCAA GATCCTACCC GGAAAGGCTC AGTGGTCAAT
    GTGAATCCTA CCAACACTAG GCCACAGAGT GACACCCCGG AGATTCGTAA ATACAAGAAG
    AGGTTTAACT CTGAGATTCT GTGTGCTGCC TTATGGGGAG TGAATTTGCT AGTGGGTACA
    GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTCTATCC TCTTATCAAC
    CGAAGACGAT TTCAACAAAT GGACGTACTT GAGGGCTTGA ATGTCTTGGT GACAATATCT
    GGCAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC
    AATGATCCAG AAGTTGAGAA GAAGCAGGGA TGGACAACCG TAGGGGATTT GGAAGGATGT
    GTACATTATA AAGTTGTAAA ATATGAAAGA ATCAAATTTC TGGTGATTGC TTTGAAGAGT
    TCTGTGGAAG TCTATGCGTG GGCACCAAAG CCATATCACA AATTTATGGC CTTTAAGTCA
    TTTGGAGAAT TGGTACATAA GCCATTACTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG
    TTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA TTCAGGATCA
    GTCTATGACA TTTATCTACC AACACATGTA AGAAAGAACC CACACTCTAT GATCCAGTGT
    AGCATCAAAC CCCATGCAAT CATCATCCTC CCCAATACAG ATGGAATGGA GCTTCTGGTG
    TGCTATGAAG ATGAGGGGGT TTATGTAAAC ACATATGGAA GGATCACCAA GGATGTAGTT
    CTACAGTGGG GAGAGATGCC TACATCAGTA GCATATATTC GATCCAATCA GACAATGGGC
    TGGGGAGAGA AGGCCATAGA GATCCGATCT GTGGAAACTG GTCACTTGGA TGGTGTGTTC
    ATGCACAAAA GGGCTCAAAG ACTAAAATTC TTGTGTGAAC GCAATGACAA GGTGTTCTTT
    GCCTCTGTTC GGTCTGGTGG CAGCAGTCAG GTTTATTTCA TGACCTTAGG CAGGACTTCT
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36839
    Clone ID Related Accession (Same CDS sequence) XM_005264060.3
    Accession Version XM_005264060.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3843bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264060.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG TATTGAGCCC AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG
    GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA GGGTCCAGCA ACTCAGGATC CCAGCCCGGG
    TCTCACCCTG GGTCTCAGAG TGGCTCCGGG GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGCT CTCCATCTCA GCGCCTGGAA AATGCAGTGA AAAAACCTGA AGATAAAAAG
    GAAGTTTTCA GACCCCTCAA GCCTGCTGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG
    GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA
    GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT
    GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG
    GAGGGCACTC TAATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCCGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC CTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT
    TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC
    AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT
    GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG
    ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT
    CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA
    GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA
    GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG
    GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT
    TATCTACCAA CACATGTAAG AAAGAACCCA CACTCTATGA TCCAGTGTAG CATCAAACCC
    CATGCAATCA TCATCCTCCC CAATACAGAT GGAATGGAGC TTCTGGTGTG CTATGAAGAT
    GAGGGGGTTT ATGTAAACAC ATATGGAAGG ATCACCAAGG ATGTAGTTCT ACAGTGGGGA
    GAGATGCCTA CATCAGTAGC ATATATTCGA TCCAATCAGA CAATGGGCTG GGGAGAGAAG
    GCCATAGAGA TCCGATCTGT GGAAACTGGT CACTTGGATG GTGTGTTCAT GCACAAAAGG
    GCTCAAAGAC TAAAATTCTT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCTGTTCGG
    TCTGGTGGCA GCAGTCAGGT TTATTTCATG ACCTTAGGCA GGACTTCTCT TCTGAGCTGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264117.1
    CDS75..3917
    Translation

    Target ORF information:

    RefSeq Version XM_005264060.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264060.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG TATTGAGCCC AGGCTTCTGT GGGAGAGAGT GGAGAAGCTG
    GTGCCCAGAC CTGGCAGTGG CAGCTCCTCA GGGTCCAGCA ACTCAGGATC CCAGCCCGGG
    TCTCACCCTG GGTCTCAGAG TGGCTCCGGG GAACGCTTCA GAGTGAGATC ATCATCCAAG
    TCTGAAGGCT CTCCATCTCA GCGCCTGGAA AATGCAGTGA AAAAACCTGA AGATAAAAAG
    GAAGTTTTCA GACCCCTCAA GCCTGCTGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG
    GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA
    GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT
    GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG
    GAGGGCACTC TAATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCCGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC CTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT
    TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC
    AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT
    GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG
    ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT
    CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA
    GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA
    GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG
    GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT
    TATCTACCAA CACATGTAAG AAAGAACCCA CACTCTATGA TCCAGTGTAG CATCAAACCC
    CATGCAATCA TCATCCTCCC CAATACAGAT GGAATGGAGC TTCTGGTGTG CTATGAAGAT
    GAGGGGGTTT ATGTAAACAC ATATGGAAGG ATCACCAAGG ATGTAGTTCT ACAGTGGGGA
    GAGATGCCTA CATCAGTAGC ATATATTCGA TCCAATCAGA CAATGGGCTG GGGAGAGAAG
    GCCATAGAGA TCCGATCTGT GGAAACTGGT CACTTGGATG GTGTGTTCAT GCACAAAAGG
    GCTCAAAGAC TAAAATTCTT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCTGTTCGG
    TCTGGTGGCA GCAGTCAGGT TTATTTCATG ACCTTAGGCA GGACTTCTCT TCTGAGCTGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36841
    Clone ID Related Accession (Same CDS sequence) XM_005264062.3
    Accession Version XM_005264062.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264062.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC
    AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC
    CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA
    GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT
    TTCAGACCCC TCAAGCCTGC TGGCGAAGTG GATCTGACCG CACTGGCCAA AGAGCTTCGA
    GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG
    TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC
    TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA
    TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC
    AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT
    GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT
    TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG
    TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG
    ATTTCTCCAT CTAGCGGAAC AACAGTGACA TCTGTGGTGG GATTTTCCTG TGATGGGATG
    AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT
    ACCAACACTA GGCCACAGAG TGACACCCCG GAGATTCGTA AATACAAGAA GAGGTTTAAC
    TCTGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC
    CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTTATCAA CCGAAGACGA
    TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG
    GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA
    GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT
    AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAGAG TTCTGTGGAA
    GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA
    TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCAGGATC AGTCTATGAC
    ATTTATCTAC CAACACATGT AAGAAAGAAC CCACACTCTA TGATCCAGTG TAGCATCAAA
    CCCCATGCAA TCATCATCCT CCCCAATACA GATGGAATGG AGCTTCTGGT GTGCTATGAA
    GATGAGGGGG TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTAGT TCTACAGTGG
    GGAGAGATGC CTACATCAGT AGCATATATT CGATCCAATC AGACAATGGG CTGGGGAGAG
    AAGGCCATAG AGATCCGATC TGTGGAAACT GGTCACTTGG ATGGTGTGTT CATGCACAAA
    AGGGCTCAAA GACTAAAATT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCTGTT
    CGGTCTGGTG GCAGCAGTCA GGTTTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264119.1
    CDS75..3860
    Translation

    Target ORF information:

    RefSeq Version XM_005264062.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264062.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC
    AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC
    CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA
    GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT
    TTCAGACCCC TCAAGCCTGC TGGCGAAGTG GATCTGACCG CACTGGCCAA AGAGCTTCGA
    GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG
    TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC
    TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA
    TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC
    AAGGAGGGCA CTCTAATCGT CCGCCAGAGT ACAGTTGACC AAAAGCGTGC CAGCCATCAT
    GAGAGCAATG GCTTTGCCGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT
    TCCTCCTCCA CTTCCTCCAC CTCCTCCTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG
    TCCCCACAGA CACCCCAGGA CAAGCTCACT GCTAATGAGA CTCAGTCCGC TAGTAGCACA
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG
    ATTTCTCCAT CTAGCGGAAC AACAGTGACA TCTGTGGTGG GATTTTCCTG TGATGGGATG
    AGACCAGAAG CCATAAGGCA AGATCCTACC CGGAAAGGCT CAGTGGTCAA TGTGAATCCT
    ACCAACACTA GGCCACAGAG TGACACCCCG GAGATTCGTA AATACAAGAA GAGGTTTAAC
    TCTGAGATTC TGTGTGCTGC CTTATGGGGA GTGAATTTGC TAGTGGGTAC AGAGAGTGGC
    CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTCTATC CTCTTATCAA CCGAAGACGA
    TTTCAACAAA TGGACGTACT TGAGGGCTTG AATGTCTTGG TGACAATATC TGGCAAAAAG
    GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAATGATCCA
    GAAGTTGAGA AGAAGCAGGG ATGGACAACC GTAGGGGATT TGGAAGGATG TGTACATTAT
    AAAGTTGTAA AATATGAAAG AATCAAATTT CTGGTGATTG CTTTGAAGAG TTCTGTGGAA
    GTCTATGCGT GGGCACCAAA GCCATATCAC AAATTTATGG CCTTTAAGTC ATTTGGAGAA
    TTGGTACATA AGCCATTACT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GTTGAAAGTG
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ATTCAGGATC AGTCTATGAC
    ATTTATCTAC CAACACATGT AAGAAAGAAC CCACACTCTA TGATCCAGTG TAGCATCAAA
    CCCCATGCAA TCATCATCCT CCCCAATACA GATGGAATGG AGCTTCTGGT GTGCTATGAA
    GATGAGGGGG TTTATGTAAA CACATATGGA AGGATCACCA AGGATGTAGT TCTACAGTGG
    GGAGAGATGC CTACATCAGT AGCATATATT CGATCCAATC AGACAATGGG CTGGGGAGAG
    AAGGCCATAG AGATCCGATC TGTGGAAACT GGTCACTTGG ATGGTGTGTT CATGCACAAA
    AGGGCTCAAA GACTAAAATT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCTGTT
    CGGTCTGGTG GCAGCAGTCA GGTTTATTTC ATGACCTTAG GCAGGACTTC TCTTCTGAGC
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36842
    Clone ID Related Accession (Same CDS sequence) XM_005264063.3
    Accession Version XM_005264063.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3783bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264063.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA
    CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT
    GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC
    TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC
    AGACCCCTCA AGCCTGCTGG CGAAGTGGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG
    GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA
    GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT
    GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG
    GAGGGCACTC TAATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCCGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC CTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT
    TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC
    AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT
    GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG
    ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT
    CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA
    GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA
    GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG
    GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT
    TATCTACCAA CACATGTAAG AAAGAACCCA CACTCTATGA TCCAGTGTAG CATCAAACCC
    CATGCAATCA TCATCCTCCC CAATACAGAT GGAATGGAGC TTCTGGTGTG CTATGAAGAT
    GAGGGGGTTT ATGTAAACAC ATATGGAAGG ATCACCAAGG ATGTAGTTCT ACAGTGGGGA
    GAGATGCCTA CATCAGTAGC ATATATTCGA TCCAATCAGA CAATGGGCTG GGGAGAGAAG
    GCCATAGAGA TCCGATCTGT GGAAACTGGT CACTTGGATG GTGTGTTCAT GCACAAAAGG
    GCTCAAAGAC TAAAATTCTT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCTGTTCGG
    TCTGGTGGCA GCAGTCAGGT TTATTTCATG ACCTTAGGCA GGACTTCTCT TCTGAGCTGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264120.1
    CDS75..3857
    Translation

    Target ORF information:

    RefSeq Version XM_005264063.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264063.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA
    CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT
    GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC
    TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC
    AGACCCCTCA AGCCTGCTGG CGAAGTGGAT CTGACCGCAC TGGCCAAAGA GCTTCGAGCA
    GTGGAAGATG TACGGCCACC TCACAAAGTA ACGGACTACT CCTCATCCAG TGAGGAGTCG
    GGGACGACGG ATGAGGAGGA CGACGATGTG GAGCAGGAAG GGGCTGACGA GTCCACCTCA
    GGACCAGAGG ACACCAGAGC AGCGTCATCT CTGAATTTGA GCAATGGTGA AACGGAATCT
    GTGAAAACCA TGATTGTCCA TGATGATGTA GAAAGTGAGC CGGCCATGAC CCCATCCAAG
    GAGGGCACTC TAATCGTCCG CCAGAGTACA GTTGACCAAA AGCGTGCCAG CCATCATGAG
    AGCAATGGCT TTGCCGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC
    TCCTCCACTT CCTCCACCTC CTCCTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC
    CCACAGACAC CCCAGGACAA GCTCACTGCT AATGAGACTC AGTCCGCTAG TAGCACACTC
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT
    TCTCCATCTA GCGGAACAAC AGTGACATCT GTGGTGGGAT TTTCCTGTGA TGGGATGAGA
    CCAGAAGCCA TAAGGCAAGA TCCTACCCGG AAAGGCTCAG TGGTCAATGT GAATCCTACC
    AACACTAGGC CACAGAGTGA CACCCCGGAG ATTCGTAAAT ACAAGAAGAG GTTTAACTCT
    GAGATTCTGT GTGCTGCCTT ATGGGGAGTG AATTTGCTAG TGGGTACAGA GAGTGGCCTG
    ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTCTATCCTC TTATCAACCG AAGACGATTT
    CAACAAATGG ACGTACTTGA GGGCTTGAAT GTCTTGGTGA CAATATCTGG CAAAAAGGAT
    AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA TGATCCAGAA
    GTTGAGAAGA AGCAGGGATG GACAACCGTA GGGGATTTGG AAGGATGTGT ACATTATAAA
    GTTGTAAAAT ATGAAAGAAT CAAATTTCTG GTGATTGCTT TGAAGAGTTC TGTGGAAGTC
    TATGCGTGGG CACCAAAGCC ATATCACAAA TTTATGGCCT TTAAGTCATT TGGAGAATTG
    GTACATAAGC CATTACTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGTT GAAAGTGATC
    TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGATT CAGGATCAGT CTATGACATT
    TATCTACCAA CACATGTAAG AAAGAACCCA CACTCTATGA TCCAGTGTAG CATCAAACCC
    CATGCAATCA TCATCCTCCC CAATACAGAT GGAATGGAGC TTCTGGTGTG CTATGAAGAT
    GAGGGGGTTT ATGTAAACAC ATATGGAAGG ATCACCAAGG ATGTAGTTCT ACAGTGGGGA
    GAGATGCCTA CATCAGTAGC ATATATTCGA TCCAATCAGA CAATGGGCTG GGGAGAGAAG
    GCCATAGAGA TCCGATCTGT GGAAACTGGT CACTTGGATG GTGTGTTCAT GCACAAAAGG
    GCTCAAAGAC TAAAATTCTT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCTGTTCGG
    TCTGGTGGCA GCAGTCAGGT TTATTTCATG ACCTTAGGCA GGACTTCTCT TCTGAGCTGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36843
    Clone ID Related Accession (Same CDS sequence) XM_005264064.3
    Accession Version XM_005264064.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3777bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264064.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC
    AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC
    CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA
    GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT
    TTCAGACCCC TCAAGCCTGC TGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA
    GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG
    ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA
    GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA
    ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC
    ACTCTAATCG TCCGCCAGAG TACAGTTGAC CAAAAGCGTG CCAGCCATCA TGAGAGCAAT
    GGCTTTGCCG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC
    ACTTCCTCCA CCTCCTCCTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG
    ACACCCCAGG ACAAGCTCAC TGCTAATGAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA
    TCTAGCGGAA CAACAGTGAC ATCTGTGGTG GGATTTTCCT GTGATGGGAT GAGACCAGAA
    GCCATAAGGC AAGATCCTAC CCGGAAAGGC TCAGTGGTCA ATGTGAATCC TACCAACACT
    AGGCCACAGA GTGACACCCC GGAGATTCGT AAATACAAGA AGAGGTTTAA CTCTGAGATT
    CTGTGTGCTG CCTTATGGGG AGTGAATTTG CTAGTGGGTA CAGAGAGTGG CCTGATGCTG
    CTGGACAGAA GTGGCCAAGG GAAGGTCTAT CCTCTTATCA ACCGAAGACG ATTTCAACAA
    ATGGACGTAC TTGAGGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAATGATCC AGAAGTTGAG
    AAGAAGCAGG GATGGACAAC CGTAGGGGAT TTGGAAGGAT GTGTACATTA TAAAGTTGTA
    AAATATGAAA GAATCAAATT TCTGGTGATT GCTTTGAAGA GTTCTGTGGA AGTCTATGCG
    TGGGCACCAA AGCCATATCA CAAATTTATG GCCTTTAAGT CATTTGGAGA ATTGGTACAT
    AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GATTCAGGAT CAGTCTATGA CATTTATCTA
    CCAACACATG TAAGAAAGAA CCCACACTCT ATGATCCAGT GTAGCATCAA ACCCCATGCA
    ATCATCATCC TCCCCAATAC AGATGGAATG GAGCTTCTGG TGTGCTATGA AGATGAGGGG
    GTTTATGTAA ACACATATGG AAGGATCACC AAGGATGTAG TTCTACAGTG GGGAGAGATG
    CCTACATCAG TAGCATATAT TCGATCCAAT CAGACAATGG GCTGGGGAGA GAAGGCCATA
    GAGATCCGAT CTGTGGAAAC TGGTCACTTG GATGGTGTGT TCATGCACAA AAGGGCTCAA
    AGACTAAAAT TCTTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT
    GGCAGCAGTC AGGTTTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264121.1
    CDS75..3851
    Translation

    Target ORF information:

    RefSeq Version XM_005264064.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264064.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC
    AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC
    CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA
    GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT
    TTCAGACCCC TCAAGCCTGC TGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA
    GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG
    ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA
    GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA
    ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC
    ACTCTAATCG TCCGCCAGAG TACAGTTGAC CAAAAGCGTG CCAGCCATCA TGAGAGCAAT
    GGCTTTGCCG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC
    ACTTCCTCCA CCTCCTCCTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG
    ACACCCCAGG ACAAGCTCAC TGCTAATGAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA
    TCTAGCGGAA CAACAGTGAC ATCTGTGGTG GGATTTTCCT GTGATGGGAT GAGACCAGAA
    GCCATAAGGC AAGATCCTAC CCGGAAAGGC TCAGTGGTCA ATGTGAATCC TACCAACACT
    AGGCCACAGA GTGACACCCC GGAGATTCGT AAATACAAGA AGAGGTTTAA CTCTGAGATT
    CTGTGTGCTG CCTTATGGGG AGTGAATTTG CTAGTGGGTA CAGAGAGTGG CCTGATGCTG
    CTGGACAGAA GTGGCCAAGG GAAGGTCTAT CCTCTTATCA ACCGAAGACG ATTTCAACAA
    ATGGACGTAC TTGAGGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAATGATCC AGAAGTTGAG
    AAGAAGCAGG GATGGACAAC CGTAGGGGAT TTGGAAGGAT GTGTACATTA TAAAGTTGTA
    AAATATGAAA GAATCAAATT TCTGGTGATT GCTTTGAAGA GTTCTGTGGA AGTCTATGCG
    TGGGCACCAA AGCCATATCA CAAATTTATG GCCTTTAAGT CATTTGGAGA ATTGGTACAT
    AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GATTCAGGAT CAGTCTATGA CATTTATCTA
    CCAACACATG TAAGAAAGAA CCCACACTCT ATGATCCAGT GTAGCATCAA ACCCCATGCA
    ATCATCATCC TCCCCAATAC AGATGGAATG GAGCTTCTGG TGTGCTATGA AGATGAGGGG
    GTTTATGTAA ACACATATGG AAGGATCACC AAGGATGTAG TTCTACAGTG GGGAGAGATG
    CCTACATCAG TAGCATATAT TCGATCCAAT CAGACAATGG GCTGGGGAGA GAAGGCCATA
    GAGATCCGAT CTGTGGAAAC TGGTCACTTG GATGGTGTGT TCATGCACAA AAGGGCTCAA
    AGACTAAAAT TCTTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT
    GGCAGCAGTC AGGTTTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36844
    Clone ID Related Accession (Same CDS sequence) XM_005264065.3
    Accession Version XM_005264065.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264065.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG
    AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT
    CTGACCGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TACGGCCACC TCACAAAGTA
    ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG
    GAGCAGGAAG GGGCTGACGA GTCCACCTCA GGACCAGAGG ACACCAGAGC AGCGTCATCT
    CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA
    GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGAGTACA
    GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCCGGTCG CATTCACCTC
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCCTCCCCA
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT
    AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GCGGAACAAC AGTGACATCT
    GTGGTGGGAT TTTCCTGTGA TGGGATGAGA CCAGAAGCCA TAAGGCAAGA TCCTACCCGG
    AAAGGCTCAG TGGTCAATGT GAATCCTACC AACACTAGGC CACAGAGTGA CACCCCGGAG
    ATTCGTAAAT ACAAGAAGAG GTTTAACTCT GAGATTCTGT GTGCTGCCTT ATGGGGAGTG
    AATTTGCTAG TGGGTACAGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG
    GTCTATCCTC TTATCAACCG AAGACGATTT CAACAAATGG ACGTACTTGA GGGCTTGAAT
    GTCTTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA
    AGAAATAAAA TACTTCACAA TGATCCAGAA GTTGAGAAGA AGCAGGGATG GACAACCGTA
    GGGGATTTGG AAGGATGTGT ACATTATAAA GTTGTAAAAT ATGAAAGAAT CAAATTTCTG
    GTGATTGCTT TGAAGAGTTC TGTGGAAGTC TATGCGTGGG CACCAAAGCC ATATCACAAA
    TTTATGGCCT TTAAGTCATT TGGAGAATTG GTACATAAGC CATTACTGGT GGATCTCACT
    GTTGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT
    GATGTGGATT CAGGATCAGT CTATGACATT TATCTACCAA CACATGTAAG AAAGAACCCA
    CACTCTATGA TCCAGTGTAG CATCAAACCC CATGCAATCA TCATCCTCCC CAATACAGAT
    GGAATGGAGC TTCTGGTGTG CTATGAAGAT GAGGGGGTTT ATGTAAACAC ATATGGAAGG
    ATCACCAAGG ATGTAGTTCT ACAGTGGGGA GAGATGCCTA CATCAGTAGC ATATATTCGA
    TCCAATCAGA CAATGGGCTG GGGAGAGAAG GCCATAGAGA TCCGATCTGT GGAAACTGGT
    CACTTGGATG GTGTGTTCAT GCACAAAAGG GCTCAAAGAC TAAAATTCTT GTGTGAACGC
    AATGACAAGG TGTTCTTTGC CTCTGTTCGG TCTGGTGGCA GCAGTCAGGT TTATTTCATG
    ACCTTAGGCA GGACTTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264122.1
    CDS75..3767
    Translation

    Target ORF information:

    RefSeq Version XM_005264065.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264065.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG
    AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT
    CTGACCGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TACGGCCACC TCACAAAGTA
    ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG
    GAGCAGGAAG GGGCTGACGA GTCCACCTCA GGACCAGAGG ACACCAGAGC AGCGTCATCT
    CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA
    GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGAGTACA
    GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCCGGTCG CATTCACCTC
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCCTCCCCA
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT
    AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GCGGAACAAC AGTGACATCT
    GTGGTGGGAT TTTCCTGTGA TGGGATGAGA CCAGAAGCCA TAAGGCAAGA TCCTACCCGG
    AAAGGCTCAG TGGTCAATGT GAATCCTACC AACACTAGGC CACAGAGTGA CACCCCGGAG
    ATTCGTAAAT ACAAGAAGAG GTTTAACTCT GAGATTCTGT GTGCTGCCTT ATGGGGAGTG
    AATTTGCTAG TGGGTACAGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG
    GTCTATCCTC TTATCAACCG AAGACGATTT CAACAAATGG ACGTACTTGA GGGCTTGAAT
    GTCTTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA
    AGAAATAAAA TACTTCACAA TGATCCAGAA GTTGAGAAGA AGCAGGGATG GACAACCGTA
    GGGGATTTGG AAGGATGTGT ACATTATAAA GTTGTAAAAT ATGAAAGAAT CAAATTTCTG
    GTGATTGCTT TGAAGAGTTC TGTGGAAGTC TATGCGTGGG CACCAAAGCC ATATCACAAA
    TTTATGGCCT TTAAGTCATT TGGAGAATTG GTACATAAGC CATTACTGGT GGATCTCACT
    GTTGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT
    GATGTGGATT CAGGATCAGT CTATGACATT TATCTACCAA CACATGTAAG AAAGAACCCA
    CACTCTATGA TCCAGTGTAG CATCAAACCC CATGCAATCA TCATCCTCCC CAATACAGAT
    GGAATGGAGC TTCTGGTGTG CTATGAAGAT GAGGGGGTTT ATGTAAACAC ATATGGAAGG
    ATCACCAAGG ATGTAGTTCT ACAGTGGGGA GAGATGCCTA CATCAGTAGC ATATATTCGA
    TCCAATCAGA CAATGGGCTG GGGAGAGAAG GCCATAGAGA TCCGATCTGT GGAAACTGGT
    CACTTGGATG GTGTGTTCAT GCACAAAAGG GCTCAAAGAC TAAAATTCTT GTGTGAACGC
    AATGACAAGG TGTTCTTTGC CTCTGTTCGG TCTGGTGGCA GCAGTCAGGT TTATTTCATG
    ACCTTAGGCA GGACTTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36845
    Clone ID Related Accession (Same CDS sequence) XM_005264066.3
    Accession Version XM_005264066.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X32
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264066.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGTA TTGAGCCCAG GCTTCTGTGG
    GAGAGAGTGG AGAAGCTGGT GCCCAGACCT GGCAGTGGCA GCTCCTCAGG GTCCAGCAAC
    TCAGGATCCC AGCCCGGGTC TCACCCTGGG TCTCAGAGTG GCTCCGGGGA ACGCTTCAGA
    GTGAGATCAT CATCCAAGTC TGAAGGCTCT CCATCTCAGC GCCTGGAAAA TGCAGTGAAA
    AAACCTGAAG ATAAAAAGGA AGTTTTCAGA CCCCTCAAGC CTGCTGGCGA AGTGGATCTG
    ACCGCACTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTAC GGCCACCTCA CAAAGTAACG
    GACTACTCCT CATCCAGTGA GGAGTCGGGG ACGACGGATG AGGAGGACGA CGATGTGGAG
    CAGGAAGGGG CTGACGAGTC CACCTCAGGA CCAGAGGACA CCAGAGCAGC GTCATCTCTG
    AATTTGAGCA ATGGTGAAAC GGAATCTGTG AAAACCATGA TTGTCCATGA TGATGTAGAA
    AGTGAGCCGG CCATGACCCC ATCCAAGGAG GGCACTCTAA TCGTCCGCCA GAGTACAGTT
    GACCAAAAGC GTGCCAGCCA TCATGAGAGC AATGGCTTTG CCGGTCGCAT TCACCTCTTG
    CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCCTC CTCCCCATCC
    TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTGCTAAT
    GAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT
    TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGCG GAACAACAGT GACATCTGTG
    GTGGGATTTT CCTGTGATGG GATGAGACCA GAAGCCATAA GGCAAGATCC TACCCGGAAA
    GGCTCAGTGG TCAATGTGAA TCCTACCAAC ACTAGGCCAC AGAGTGACAC CCCGGAGATT
    CGTAAATACA AGAAGAGGTT TAACTCTGAG ATTCTGTGTG CTGCCTTATG GGGAGTGAAT
    TTGCTAGTGG GTACAGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTC
    TATCCTCTTA TCAACCGAAG ACGATTTCAA CAAATGGACG TACTTGAGGG CTTGAATGTC
    TTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA
    AATAAAATAC TTCACAATGA TCCAGAAGTT GAGAAGAAGC AGGGATGGAC AACCGTAGGG
    GATTTGGAAG GATGTGTACA TTATAAAGTT GTAAAATATG AAAGAATCAA ATTTCTGGTG
    ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT GCGTGGGCAC CAAAGCCATA TCACAAATTT
    ATGGCCTTTA AGTCATTTGG AGAATTGGTA CATAAGCCAT TACTGGTGGA TCTCACTGTT
    GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT
    GTGGATTCAG GATCAGTCTA TGACATTTAT CTACCAACAC ATGTAAGAAA GAACCCACAC
    TCTATGATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA
    ATGGAGCTTC TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC
    ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACAT CAGTAGCATA TATTCGATCC
    AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC
    TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT
    GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC
    TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264123.1
    CDS75..3764
    Translation

    Target ORF information:

    RefSeq Version XM_005264066.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264066.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGTA TTGAGCCCAG GCTTCTGTGG
    GAGAGAGTGG AGAAGCTGGT GCCCAGACCT GGCAGTGGCA GCTCCTCAGG GTCCAGCAAC
    TCAGGATCCC AGCCCGGGTC TCACCCTGGG TCTCAGAGTG GCTCCGGGGA ACGCTTCAGA
    GTGAGATCAT CATCCAAGTC TGAAGGCTCT CCATCTCAGC GCCTGGAAAA TGCAGTGAAA
    AAACCTGAAG ATAAAAAGGA AGTTTTCAGA CCCCTCAAGC CTGCTGGCGA AGTGGATCTG
    ACCGCACTGG CCAAAGAGCT TCGAGCAGTG GAAGATGTAC GGCCACCTCA CAAAGTAACG
    GACTACTCCT CATCCAGTGA GGAGTCGGGG ACGACGGATG AGGAGGACGA CGATGTGGAG
    CAGGAAGGGG CTGACGAGTC CACCTCAGGA CCAGAGGACA CCAGAGCAGC GTCATCTCTG
    AATTTGAGCA ATGGTGAAAC GGAATCTGTG AAAACCATGA TTGTCCATGA TGATGTAGAA
    AGTGAGCCGG CCATGACCCC ATCCAAGGAG GGCACTCTAA TCGTCCGCCA GAGTACAGTT
    GACCAAAAGC GTGCCAGCCA TCATGAGAGC AATGGCTTTG CCGGTCGCAT TCACCTCTTG
    CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCCTC CTCCCCATCC
    TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTGCTAAT
    GAGACTCAGT CCGCTAGTAG CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT
    TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGCG GAACAACAGT GACATCTGTG
    GTGGGATTTT CCTGTGATGG GATGAGACCA GAAGCCATAA GGCAAGATCC TACCCGGAAA
    GGCTCAGTGG TCAATGTGAA TCCTACCAAC ACTAGGCCAC AGAGTGACAC CCCGGAGATT
    CGTAAATACA AGAAGAGGTT TAACTCTGAG ATTCTGTGTG CTGCCTTATG GGGAGTGAAT
    TTGCTAGTGG GTACAGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTC
    TATCCTCTTA TCAACCGAAG ACGATTTCAA CAAATGGACG TACTTGAGGG CTTGAATGTC
    TTGGTGACAA TATCTGGCAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA
    AATAAAATAC TTCACAATGA TCCAGAAGTT GAGAAGAAGC AGGGATGGAC AACCGTAGGG
    GATTTGGAAG GATGTGTACA TTATAAAGTT GTAAAATATG AAAGAATCAA ATTTCTGGTG
    ATTGCTTTGA AGAGTTCTGT GGAAGTCTAT GCGTGGGCAC CAAAGCCATA TCACAAATTT
    ATGGCCTTTA AGTCATTTGG AGAATTGGTA CATAAGCCAT TACTGGTGGA TCTCACTGTT
    GAGGAAGGCC AGAGGTTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT
    GTGGATTCAG GATCAGTCTA TGACATTTAT CTACCAACAC ATGTAAGAAA GAACCCACAC
    TCTATGATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA
    ATGGAGCTTC TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC
    ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACAT CAGTAGCATA TATTCGATCC
    AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC
    TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT
    GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC
    TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36846
    Clone ID Related Accession (Same CDS sequence) XM_005264068.3
    Accession Version XM_005264068.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3684bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X33
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264068.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG
    AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGA TCTGACCGCA
    CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC
    TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA
    GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG
    AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG
    CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGAGTAC AGTTGACCAA
    AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCCGGTC GCATTCACCT CTTGCCAGAT
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCCTCCCC ATCCTCCAGC
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA
    TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA
    GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA
    TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA
    GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT
    CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA
    ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG
    GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT
    TCAGGATCAG TCTATGACAT TTATCTACCA ACACATGTAA GAAAGAACCC ACACTCTATG
    ATCCAGTGTA GCATCAAACC CCATGCAATC ATCATCCTCC CCAATACAGA TGGAATGGAG
    CTTCTGGTGT GCTATGAAGA TGAGGGGGTT TATGTAAACA CATATGGAAG GATCACCAAG
    GATGTAGTTC TACAGTGGGG AGAGATGCCT ACATCAGTAG CATATATTCG ATCCAATCAG
    ACAATGGGCT GGGGAGAGAA GGCCATAGAG ATCCGATCTG TGGAAACTGG TCACTTGGAT
    GGTGTGTTCA TGCACAAAAG GGCTCAAAGA CTAAAATTCT TGTGTGAACG CAATGACAAG
    GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC AGCAGTCAGG TTTATTTCAT GACCTTAGGC
    AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264125.1
    CDS75..3758
    Translation

    Target ORF information:

    RefSeq Version XM_005264068.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X33, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264068.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG
    AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGA TCTGACCGCA
    CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT GTACGGCCAC CTCACAAAGT AACGGACTAC
    TCCTCATCCA GTGAGGAGTC GGGGACGACG GATGAGGAGG ACGACGATGT GGAGCAGGAA
    GGGGCTGACG AGTCCACCTC AGGACCAGAG GACACCAGAG CAGCGTCATC TCTGAATTTG
    AGCAATGGTG AAACGGAATC TGTGAAAACC ATGATTGTCC ATGATGATGT AGAAAGTGAG
    CCGGCCATGA CCCCATCCAA GGAGGGCACT CTAATCGTCC GCCAGAGTAC AGTTGACCAA
    AAGCGTGCCA GCCATCATGA GAGCAATGGC TTTGCCGGTC GCATTCACCT CTTGCCAGAT
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCCTCCCC ATCCTCCAGC
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTGC TAATGAGACT
    CAGTCCGCTA GTAGCACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGAACAA CAGTGACATC TGTGGTGGGA
    TTTTCCTGTG ATGGGATGAG ACCAGAAGCC ATAAGGCAAG ATCCTACCCG GAAAGGCTCA
    GTGGTCAATG TGAATCCTAC CAACACTAGG CCACAGAGTG ACACCCCGGA GATTCGTAAA
    TACAAGAAGA GGTTTAACTC TGAGATTCTG TGTGCTGCCT TATGGGGAGT GAATTTGCTA
    GTGGGTACAG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTCTATCCT
    CTTATCAACC GAAGACGATT TCAACAAATG GACGTACTTG AGGGCTTGAA TGTCTTGGTG
    ACAATATCTG GCAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA
    ATACTTCACA ATGATCCAGA AGTTGAGAAG AAGCAGGGAT GGACAACCGT AGGGGATTTG
    GAAGGATGTG TACATTATAA AGTTGTAAAA TATGAAAGAA TCAAATTTCT GGTGATTGCT
    TTGAAGAGTT CTGTGGAAGT CTATGCGTGG GCACCAAAGC CATATCACAA ATTTATGGCC
    TTTAAGTCAT TTGGAGAATT GGTACATAAG CCATTACTGG TGGATCTCAC TGTTGAGGAA
    GGCCAGAGGT TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAT
    TCAGGATCAG TCTATGACAT TTATCTACCA ACACATGTAA GAAAGAACCC ACACTCTATG
    ATCCAGTGTA GCATCAAACC CCATGCAATC ATCATCCTCC CCAATACAGA TGGAATGGAG
    CTTCTGGTGT GCTATGAAGA TGAGGGGGTT TATGTAAACA CATATGGAAG GATCACCAAG
    GATGTAGTTC TACAGTGGGG AGAGATGCCT ACATCAGTAG CATATATTCG ATCCAATCAG
    ACAATGGGCT GGGGAGAGAA GGCCATAGAG ATCCGATCTG TGGAAACTGG TCACTTGGAT
    GGTGTGTTCA TGCACAAAAG GGCTCAAAGA CTAAAATTCT TGTGTGAACG CAATGACAAG
    GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC AGCAGTCAGG TTTATTTCAT GACCTTAGGC
    AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36847
    Clone ID Related Accession (Same CDS sequence) XM_005264069.2
    Accession Version XM_005264069.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X35
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264069.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC
    GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC
    AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA
    AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GATCTGACCG CACTGGCCAA AGAGCTTCGA
    GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG
    TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC
    TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA
    TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC
    AAGGAGGGCA CTCTAATCGT CCGCCAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT
    AGCGGAACAA CAGTGACATC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG
    CCACAGAGTG ACACCCCGGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAGATTCTG
    TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG
    GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTTATCAACC GAAGACGATT TCAACAAATG
    GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG
    AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA
    TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCGTGG
    GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG
    CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCAGGATCAG TCTATGACAT TTATCTACCA
    ACACATATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA
    ATGGAGCTTC TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC
    ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACAT CAGTAGCATA TATTCGATCC
    AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC
    TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT
    GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC
    TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264126.1
    CDS75..3704
    Translation

    Target ORF information:

    RefSeq Version XM_005264069.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X35, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264069.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTGGAAGAT
    AGATTTAGGA AAACTAACCA CAGCTCCCCT GAAGCCCAGT CTAAGCAGAC AGGCAGAGTA
    TTGGAGCCAC CAGTGCCTTC CCGATCAGAG TCTTTTTCCA ATGGCAACTC CGAGTCTGTG
    CATCCCGCCC TGCAGAGACC AGCGGAGCCA CAGGTTCCTG TGAGAACAAC ATCTCGCTCC
    CCTGTTCTGT CCCGTCGAGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAA TAGCCAGGCA
    GGACAGAGAA ACTCCACCAG CAGTATTGAG CCCAGGCTTC TGTGGGAGAG AGTGGAGAAG
    CTGGTGCCCA GACCTGGCAG TGGCAGCTCC TCAGGGTCCA GCAACTCAGG ATCCCAGCCC
    GGGTCTCACC CTGGGTCTCA GAGTGGCTCC GGGGAACGCT TCAGAGTGAG ATCATCATCC
    AAGTCTGAAG GCTCTCCATC TCAGCGCCTG GAAAATGCAG TGAAAAAACC TGAAGATAAA
    AAGGAAGTTT TCAGACCCCT CAAGCCTGCT GATCTGACCG CACTGGCCAA AGAGCTTCGA
    GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG
    TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC
    TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA
    TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC
    AAGGAGGGCA CTCTAATCGT CCGCCAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT
    AGCGGAACAA CAGTGACATC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG
    CCACAGAGTG ACACCCCGGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAGATTCTG
    TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG
    GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTTATCAACC GAAGACGATT TCAACAAATG
    GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG
    AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA
    TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCGTGG
    GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG
    CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCAGGATCAG TCTATGACAT TTATCTACCA
    ACACATATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA
    ATGGAGCTTC TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC
    ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACAT CAGTAGCATA TATTCGATCC
    AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC
    TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT
    GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC
    TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36848
    Clone ID Related Accession (Same CDS sequence) XM_005264071.2
    Accession Version XM_005264071.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X40
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264071.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA
    CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT
    GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC
    TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC
    AGACCCCTCA AGCCTGCTGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG
    GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG
    GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC
    ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT
    CTAATCGTCC GCCAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGAACAACA
    GTGACATCTG TGGTGGGATT TTCCTGTGAT GGGATGAGAC CAGAAGCCAT AAGGCAAGAT
    CCTACCCGGA AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAC
    ACCCCGGAGA TTCGTAAATA CAAGAAGAGG TTTAACTCTG AGATTCTGTG TGCTGCCTTA
    TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC
    CAAGGGAAGG TCTATCCTCT TATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG
    GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG
    TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG
    ACAACCGTAG GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC
    AAATTTCTGG TGATTGCTTT GAAGAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA
    TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG
    GATCTCACTG TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC
    CATGCTGTTG ATGTGGATTC AGGATCAGTC TATGACATTT ATCTACCAAC ACATATCCAG
    TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG
    GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA
    GTTCTACAGT GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG
    GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG
    TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC
    TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264128.1
    CDS75..3632
    Translation

    Target ORF information:

    RefSeq Version XM_005264071.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X40, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264071.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GTATTGAGCC CAGGCTTCTG TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA
    CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC AACTCAGGAT CCCAGCCCGG GTCTCACCCT
    GGGTCTCAGA GTGGCTCCGG GGAACGCTTC AGAGTGAGAT CATCATCCAA GTCTGAAGGC
    TCTCCATCTC AGCGCCTGGA AAATGCAGTG AAAAAACCTG AAGATAAAAA GGAAGTTTTC
    AGACCCCTCA AGCCTGCTGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG
    GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG
    GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC
    ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT
    CTAATCGTCC GCCAGACTCA GTCCGCTAGT AGCACACTCC AGAAACACAA ATCTTCCTCC
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGAACAACA
    GTGACATCTG TGGTGGGATT TTCCTGTGAT GGGATGAGAC CAGAAGCCAT AAGGCAAGAT
    CCTACCCGGA AAGGCTCAGT GGTCAATGTG AATCCTACCA ACACTAGGCC ACAGAGTGAC
    ACCCCGGAGA TTCGTAAATA CAAGAAGAGG TTTAACTCTG AGATTCTGTG TGCTGCCTTA
    TGGGGAGTGA ATTTGCTAGT GGGTACAGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC
    CAAGGGAAGG TCTATCCTCT TATCAACCGA AGACGATTTC AACAAATGGA CGTACTTGAG
    GGCTTGAATG TCTTGGTGAC AATATCTGGC AAAAAGGATA AGTTACGTGT CTACTATTTG
    TCCTGGTTAA GAAATAAAAT ACTTCACAAT GATCCAGAAG TTGAGAAGAA GCAGGGATGG
    ACAACCGTAG GGGATTTGGA AGGATGTGTA CATTATAAAG TTGTAAAATA TGAAAGAATC
    AAATTTCTGG TGATTGCTTT GAAGAGTTCT GTGGAAGTCT ATGCGTGGGC ACCAAAGCCA
    TATCACAAAT TTATGGCCTT TAAGTCATTT GGAGAATTGG TACATAAGCC ATTACTGGTG
    GATCTCACTG TTGAGGAAGG CCAGAGGTTG AAAGTGATCT ATGGATCCTG TGCTGGATTC
    CATGCTGTTG ATGTGGATTC AGGATCAGTC TATGACATTT ATCTACCAAC ACATATCCAG
    TGTAGCATCA AACCCCATGC AATCATCATC CTCCCCAATA CAGATGGAAT GGAGCTTCTG
    GTGTGCTATG AAGATGAGGG GGTTTATGTA AACACATATG GAAGGATCAC CAAGGATGTA
    GTTCTACAGT GGGGAGAGAT GCCTACATCA GTAGCATATA TTCGATCCAA TCAGACAATG
    GGCTGGGGAG AGAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGTCACTT GGATGGTGTG
    TTCATGCACA AAAGGGCTCA AAGACTAAAA TTCTTGTGTG AACGCAATGA CAAGGTGTTC
    TTTGCCTCTG TTCGGTCTGG TGGCAGCAGT CAGGTTTATT TCATGACCTT AGGCAGGACT
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36850
    Clone ID Related Accession (Same CDS sequence) XM_006712868.2
    Accession Version XM_006712868.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X45
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006712868.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG
    AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT
    CTGACCGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TACGGCCACC TCACAAAGTA
    ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG
    GAGCAGGAAG GGGCTGACGA GTCCACCTCA GGACCAGAGG ACACCAGAGC AGCGTCATCT
    CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA
    GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGAGTACA
    GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCCGGTCG CATTCACCTC
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCCTCCCCA
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT
    AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GCGGAACAAC AGTGACATCT
    GTGGTGGGAT TTTCCTGTGA TGGGATGAGA CCAGAAGCCA TAAGGCAAGA TCCTACCCGG
    AAAGGCTCAG TGGTCAATGT GAATCCTACC AACACTAGGC CACAGAGTGA CACCCCGGAG
    ATTCGTAAAT ACAAGAAGAG GTTTAACTCT GAGATTCTGT GTGCTGCCTT ATGGGGAGTG
    AATTTGCTAG TGGGTACAGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG
    GTCTATCCTC TTATCAACCG AAGACGATTT CAACAAATGG ACGTACTTGA GGGCTTGAAT
    GTCTTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA
    AGAAATAAAA TACTTCACAA TGATCCAGAA GTTGAGAAGA AGCAGGGATG GACAACCGTA
    GGGGATTTGG AAGGATGTGT ACATTATAAA GTTGTAAAAT ATGAAAGAAT CAAATTTCTG
    GTGATTGCTT TGAAGAGTTC TGTGGAAGTC TATGCGTGGG CACCAAAGCC ATATCACAAA
    TTTATGGCCT TTAAGTCATT TGGAGAATTG GTACATAAGC CATTACTGGT GGATCTCACT
    GTTGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT
    GATGTGGATT CAGGATCAGT CTATGACATT TATCTACCAA CACATATCCA GTGTAGCATC
    AAACCCCATG CAATCATCAT CCTCCCCAAT ACAGATGGAA TGGAGCTTCT GGTGTGCTAT
    GAAGATGAGG GGGTTTATGT AAACACATAT GGAAGGATCA CCAAGGATGT AGTTCTACAG
    TGGGGAGAGA TGCCTACATC AGTAGCATAT ATTCGATCCA ATCAGACAAT GGGCTGGGGA
    GAGAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGTCACT TGGATGGTGT GTTCATGCAC
    AAAAGGGCTC AAAGACTAAA ATTCTTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCT
    GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT TTCATGACCT TAGGCAGGAC TTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712931.1
    CDS75..3743
    Translation

    Target ORF information:

    RefSeq Version XM_006712868.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X45, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712868.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGCATGACCA TAGGAGGCCG
    CACCCGCAGC ACTCGCAGCA GCCGCCACCA CCGCAGCAGG AAAGGAGCAA GCCAAGCTTC
    CATGCTCCCG AGCCCAAAGC CCACTACGAG CCTGCTGACC GAGCGCGAGA GGTTCCTGTG
    AGAACAACAT CTCGCTCCCC TGTTCTGTCC CGTCGAGATT CCCCACTGCA GGGCAGTGGG
    CAGCAGAATA GCCAGGCAGG ACAGAGAAAC TCCACCAGCA GTATTGAGCC CAGGCTTCTG
    TGGGAGAGAG TGGAGAAGCT GGTGCCCAGA CCTGGCAGTG GCAGCTCCTC AGGGTCCAGC
    AACTCAGGAT CCCAGCCCGG GTCTCACCCT GGGTCTCAGA GTGGCTCCGG GGAACGCTTC
    AGAGTGAGAT CATCATCCAA GTCTGAAGGC TCTCCATCTC AGCGCCTGGA AAATGCAGTG
    AAAAAACCTG AAGATAAAAA GGAAGTTTTC AGACCCCTCA AGCCTGCTGG CGAAGTGGAT
    CTGACCGCAC TGGCCAAAGA GCTTCGAGCA GTGGAAGATG TACGGCCACC TCACAAAGTA
    ACGGACTACT CCTCATCCAG TGAGGAGTCG GGGACGACGG ATGAGGAGGA CGACGATGTG
    GAGCAGGAAG GGGCTGACGA GTCCACCTCA GGACCAGAGG ACACCAGAGC AGCGTCATCT
    CTGAATTTGA GCAATGGTGA AACGGAATCT GTGAAAACCA TGATTGTCCA TGATGATGTA
    GAAAGTGAGC CGGCCATGAC CCCATCCAAG GAGGGCACTC TAATCGTCCG CCAGAGTACA
    GTTGACCAAA AGCGTGCCAG CCATCATGAG AGCAATGGCT TTGCCGGTCG CATTCACCTC
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCCTCCCCA
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTGCT
    AATGAGACTC AGTCCGCTAG TAGCACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GCGGAACAAC AGTGACATCT
    GTGGTGGGAT TTTCCTGTGA TGGGATGAGA CCAGAAGCCA TAAGGCAAGA TCCTACCCGG
    AAAGGCTCAG TGGTCAATGT GAATCCTACC AACACTAGGC CACAGAGTGA CACCCCGGAG
    ATTCGTAAAT ACAAGAAGAG GTTTAACTCT GAGATTCTGT GTGCTGCCTT ATGGGGAGTG
    AATTTGCTAG TGGGTACAGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG
    GTCTATCCTC TTATCAACCG AAGACGATTT CAACAAATGG ACGTACTTGA GGGCTTGAAT
    GTCTTGGTGA CAATATCTGG CAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA
    AGAAATAAAA TACTTCACAA TGATCCAGAA GTTGAGAAGA AGCAGGGATG GACAACCGTA
    GGGGATTTGG AAGGATGTGT ACATTATAAA GTTGTAAAAT ATGAAAGAAT CAAATTTCTG
    GTGATTGCTT TGAAGAGTTC TGTGGAAGTC TATGCGTGGG CACCAAAGCC ATATCACAAA
    TTTATGGCCT TTAAGTCATT TGGAGAATTG GTACATAAGC CATTACTGGT GGATCTCACT
    GTTGAGGAAG GCCAGAGGTT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT
    GATGTGGATT CAGGATCAGT CTATGACATT TATCTACCAA CACATATCCA GTGTAGCATC
    AAACCCCATG CAATCATCAT CCTCCCCAAT ACAGATGGAA TGGAGCTTCT GGTGTGCTAT
    GAAGATGAGG GGGTTTATGT AAACACATAT GGAAGGATCA CCAAGGATGT AGTTCTACAG
    TGGGGAGAGA TGCCTACATC AGTAGCATAT ATTCGATCCA ATCAGACAAT GGGCTGGGGA
    GAGAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGTCACT TGGATGGTGT GTTCATGCAC
    AAAAGGGCTC AAAGACTAAA ATTCTTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCT
    GTTCGGTCTG GTGGCAGCAG TCAGGTTTAT TTCATGACCT TAGGCAGGAC TTCTCTTCTG
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36851
    Clone ID Related Accession (Same CDS sequence) XM_006712869.2
    Accession Version XM_006712869.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X37
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006712869.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC
    AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC
    CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA
    GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT
    TTCAGACCCC TCAAGCCTGC TGGCGAAGTG GATCTGACCG CACTGGCCAA AGAGCTTCGA
    GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG
    TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC
    TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA
    TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC
    AAGGAGGGCA CTCTAATCGT CCGCCAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT
    AGCGGAACAA CAGTGACATC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG
    CCACAGAGTG ACACCCCGGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAGATTCTG
    TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG
    GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTTATCAACC GAAGACGATT TCAACAAATG
    GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG
    AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA
    TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCGTGG
    GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG
    CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCAGGATCAG TCTATGACAT TTATCTACCA
    ACACATATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA
    ATGGAGCTTC TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC
    ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACAT CAGTAGCATA TATTCGATCC
    AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC
    TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT
    GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC
    TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712932.1
    CDS75..3644
    Translation

    Target ORF information:

    RefSeq Version XM_006712869.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X37, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712869.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTTCCT GTGAGAACAA CATCTCGCTC CCCTGTTCTG
    TCCCGTCGAG ATTCCCCACT GCAGGGCAGT GGGCAGCAGA ATAGCCAGGC AGGACAGAGA
    AACTCCACCA GCAGTATTGA GCCCAGGCTT CTGTGGGAGA GAGTGGAGAA GCTGGTGCCC
    AGACCTGGCA GTGGCAGCTC CTCAGGGTCC AGCAACTCAG GATCCCAGCC CGGGTCTCAC
    CCTGGGTCTC AGAGTGGCTC CGGGGAACGC TTCAGAGTGA GATCATCATC CAAGTCTGAA
    GGCTCTCCAT CTCAGCGCCT GGAAAATGCA GTGAAAAAAC CTGAAGATAA AAAGGAAGTT
    TTCAGACCCC TCAAGCCTGC TGGCGAAGTG GATCTGACCG CACTGGCCAA AGAGCTTCGA
    GCAGTGGAAG ATGTACGGCC ACCTCACAAA GTAACGGACT ACTCCTCATC CAGTGAGGAG
    TCGGGGACGA CGGATGAGGA GGACGACGAT GTGGAGCAGG AAGGGGCTGA CGAGTCCACC
    TCAGGACCAG AGGACACCAG AGCAGCGTCA TCTCTGAATT TGAGCAATGG TGAAACGGAA
    TCTGTGAAAA CCATGATTGT CCATGATGAT GTAGAAAGTG AGCCGGCCAT GACCCCATCC
    AAGGAGGGCA CTCTAATCGT CCGCCAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT
    AGCGGAACAA CAGTGACATC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG
    CCACAGAGTG ACACCCCGGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAGATTCTG
    TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG
    GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTTATCAACC GAAGACGATT TCAACAAATG
    GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG
    AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA
    TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCGTGG
    GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG
    CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCAGGATCAG TCTATGACAT TTATCTACCA
    ACACATATCC AGTGTAGCAT CAAACCCCAT GCAATCATCA TCCTCCCCAA TACAGATGGA
    ATGGAGCTTC TGGTGTGCTA TGAAGATGAG GGGGTTTATG TAAACACATA TGGAAGGATC
    ACCAAGGATG TAGTTCTACA GTGGGGAGAG ATGCCTACAT CAGTAGCATA TATTCGATCC
    AATCAGACAA TGGGCTGGGG AGAGAAGGCC ATAGAGATCC GATCTGTGGA AACTGGTCAC
    TTGGATGGTG TGTTCATGCA CAAAAGGGCT CAAAGACTAA AATTCTTGTG TGAACGCAAT
    GACAAGGTGT TCTTTGCCTC TGTTCGGTCT GGTGGCAGCA GTCAGGTTTA TTTCATGACC
    TTAGGCAGGA CTTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36825
    Clone ID Related Accession (Same CDS sequence) XM_005264048.2
    Accession Version XM_005264048.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264048.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTAC AGTGGTCCCA CCTGGCATCT CTCAAGAACA ATGTTTCCCC TGTCTCGCGA
    TCCCATTCCT TCAGTGACCC TTCTCCCAAA TTTGCACACC ACCATCTTCG TTCTCAGGAC
    CCATGTCCAC CTTCCCGCAG TGAGGTGCTC AGTCAGAGCT CTGACTCTAA GTCAGAGGCG
    CCTGACCCTA CCCAAAAGGC TTGGTCTAGA TCAGACAGTG ACGAGGTGCC TCCAAGGGTT
    CCTGTGAGAA CAACATCTCG CTCCCCTGTT CTGTCCCGTC GAGATTCCCC ACTGCAGGGC
    AGTGGGCAGC AGAATAGCCA GGCAGGACAG AGAAACTCCA CCAGCAGTAT TGAGCCCAGG
    CTTCTGTGGG AGAGAGTGGA GAAGCTGGTG CCCAGACCTG GCAGTGGCAG CTCCTCAGGG
    TCCAGCAACT CAGGATCCCA GCCCGGGTCT CACCCTGGGT CTCAGAGTGG CTCCGGGGAA
    CGCTTCAGAG TGAGATCATC ATCCAAGTCT GAAGGCTCTC CATCTCAGCG CCTGGAAAAT
    GCAGTGAAAA AACCTGAAGA TAAAAAGGAA GTTTTCAGAC CCCTCAAGCC TGCTGGCGAA
    GTGGATCTGA CCGCACTGGC CAAAGAGCTT CGAGCAGTGG AAGATGTACG GCCACCTCAC
    AAAGTAACGG ACTACTCCTC ATCCAGTGAG GAGTCGGGGA CGACGGATGA GGAGGACGAC
    GATGTGGAGC AGGAAGGGGC TGACGAGTCC ACCTCAGGAC CAGAGGACAC CAGAGCAGCG
    TCATCTCTGA ATTTGAGCAA TGGTGAAACG GAATCTGTGA AAACCATGAT TGTCCATGAT
    GATGTAGAAA GTGAGCCGGC CATGACCCCA TCCAAGGAGG GCACTCTAAT CGTCCGCCAG
    AGTACAGTTG ACCAAAAGCG TGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT
    CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC
    TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC
    ACTGCTAATG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC
    TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGCGG AACAACAGTG
    ACATCTGTGG TGGGATTTTC CTGTGATGGG ATGAGACCAG AAGCCATAAG GCAAGATCCT
    ACCCGGAAAG GCTCAGTGGT CAATGTGAAT CCTACCAACA CTAGGCCACA GAGTGACACC
    CCGGAGATTC GTAAATACAA GAAGAGGTTT AACTCTGAGA TTCTGTGTGC TGCCTTATGG
    GGAGTGAATT TGCTAGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA
    GGGAAGGTCT ATCCTCTTAT CAACCGAAGA CGATTTCAAC AAATGGACGT ACTTGAGGGC
    TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC
    TGGTTAAGAA ATAAAATACT TCACAATGAT CCAGAAGTTG AGAAGAAGCA GGGATGGACA
    ACCGTAGGGG ATTTGGAAGG ATGTGTACAT TATAAAGTTG TAAAATATGA AAGAATCAAA
    TTTCTGGTGA TTGCTTTGAA GAGTTCTGTG GAAGTCTATG CGTGGGCACC AAAGCCATAT
    CACAAATTTA TGGCCTTTAA GTCATTTGGA GAATTGGTAC ATAAGCCATT ACTGGTGGAT
    CTCACTGTTG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT
    GCTGTTGATG TGGATTCAGG ATCAGTCTAT GACATTTATC TACCAACACA TATCCAGTGT
    AGCATCAAAC CCCATGCAAT CATCATCCTC CCCAATACAG ATGGAATGGA GCTTCTGGTG
    TGCTATGAAG ATGAGGGGGT TTATGTAAAC ACATATGGAA GGATCACCAA GGATGTAGTT
    CTACAGTGGG GAGAGATGCC TACATCAGTA GCATATATTC GATCCAATCA GACAATGGGC
    TGGGGAGAGA AGGCCATAGA GATCCGATCT GTGGAAACTG GTCACTTGGA TGGTGTGTTC
    ATGCACAAAA GGGCTCAAAG ACTAAAATTC TTGTGTGAAC GCAATGACAA GGTGTTCTTT
    GCCTCTGTTC GGTCTGGTGG CAGCAGTCAG GTTTATTTCA TGACCTTAGG CAGGACTTCT
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264105.1
    CDS75..4229
    Translation

    Target ORF information:

    RefSeq Version XM_005264048.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264048.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTGGAA GATAGATTTA GGAAAACTAA CCACAGCTCC
    CCTGAAGCCC AGTCTAAGCA GACAGGCAGA GTATTGGAGC CACCAGTGCC TTCCCGATCA
    GAGTCTTTTT CCAATGGCAA CTCCGAGTCT GTGCATCCCG CCCTGCAGAG ACCAGCGGAG
    CCACAGGTAC AGTGGTCCCA CCTGGCATCT CTCAAGAACA ATGTTTCCCC TGTCTCGCGA
    TCCCATTCCT TCAGTGACCC TTCTCCCAAA TTTGCACACC ACCATCTTCG TTCTCAGGAC
    CCATGTCCAC CTTCCCGCAG TGAGGTGCTC AGTCAGAGCT CTGACTCTAA GTCAGAGGCG
    CCTGACCCTA CCCAAAAGGC TTGGTCTAGA TCAGACAGTG ACGAGGTGCC TCCAAGGGTT
    CCTGTGAGAA CAACATCTCG CTCCCCTGTT CTGTCCCGTC GAGATTCCCC ACTGCAGGGC
    AGTGGGCAGC AGAATAGCCA GGCAGGACAG AGAAACTCCA CCAGCAGTAT TGAGCCCAGG
    CTTCTGTGGG AGAGAGTGGA GAAGCTGGTG CCCAGACCTG GCAGTGGCAG CTCCTCAGGG
    TCCAGCAACT CAGGATCCCA GCCCGGGTCT CACCCTGGGT CTCAGAGTGG CTCCGGGGAA
    CGCTTCAGAG TGAGATCATC ATCCAAGTCT GAAGGCTCTC CATCTCAGCG CCTGGAAAAT
    GCAGTGAAAA AACCTGAAGA TAAAAAGGAA GTTTTCAGAC CCCTCAAGCC TGCTGGCGAA
    GTGGATCTGA CCGCACTGGC CAAAGAGCTT CGAGCAGTGG AAGATGTACG GCCACCTCAC
    AAAGTAACGG ACTACTCCTC ATCCAGTGAG GAGTCGGGGA CGACGGATGA GGAGGACGAC
    GATGTGGAGC AGGAAGGGGC TGACGAGTCC ACCTCAGGAC CAGAGGACAC CAGAGCAGCG
    TCATCTCTGA ATTTGAGCAA TGGTGAAACG GAATCTGTGA AAACCATGAT TGTCCATGAT
    GATGTAGAAA GTGAGCCGGC CATGACCCCA TCCAAGGAGG GCACTCTAAT CGTCCGCCAG
    AGTACAGTTG ACCAAAAGCG TGCCAGCCAT CATGAGAGCA ATGGCTTTGC CGGTCGCATT
    CACCTCTTGC CAGATCTCTT ACAGCAAAGC CATTCCTCCT CCACTTCCTC CACCTCCTCC
    TCCCCATCCT CCAGCCAGCC GACACCCACC ATGTCCCCAC AGACACCCCA GGACAAGCTC
    ACTGCTAATG AGACTCAGTC CGCTAGTAGC ACACTCCAGA AACACAAATC TTCCTCCTCC
    TTTACACCTT TTATAGACCC CAGATTACTA CAGATTTCTC CATCTAGCGG AACAACAGTG
    ACATCTGTGG TGGGATTTTC CTGTGATGGG ATGAGACCAG AAGCCATAAG GCAAGATCCT
    ACCCGGAAAG GCTCAGTGGT CAATGTGAAT CCTACCAACA CTAGGCCACA GAGTGACACC
    CCGGAGATTC GTAAATACAA GAAGAGGTTT AACTCTGAGA TTCTGTGTGC TGCCTTATGG
    GGAGTGAATT TGCTAGTGGG TACAGAGAGT GGCCTGATGC TGCTGGACAG AAGTGGCCAA
    GGGAAGGTCT ATCCTCTTAT CAACCGAAGA CGATTTCAAC AAATGGACGT ACTTGAGGGC
    TTGAATGTCT TGGTGACAAT ATCTGGCAAA AAGGATAAGT TACGTGTCTA CTATTTGTCC
    TGGTTAAGAA ATAAAATACT TCACAATGAT CCAGAAGTTG AGAAGAAGCA GGGATGGACA
    ACCGTAGGGG ATTTGGAAGG ATGTGTACAT TATAAAGTTG TAAAATATGA AAGAATCAAA
    TTTCTGGTGA TTGCTTTGAA GAGTTCTGTG GAAGTCTATG CGTGGGCACC AAAGCCATAT
    CACAAATTTA TGGCCTTTAA GTCATTTGGA GAATTGGTAC ATAAGCCATT ACTGGTGGAT
    CTCACTGTTG AGGAAGGCCA GAGGTTGAAA GTGATCTATG GATCCTGTGC TGGATTCCAT
    GCTGTTGATG TGGATTCAGG ATCAGTCTAT GACATTTATC TACCAACACA TATCCAGTGT
    AGCATCAAAC CCCATGCAAT CATCATCCTC CCCAATACAG ATGGAATGGA GCTTCTGGTG
    TGCTATGAAG ATGAGGGGGT TTATGTAAAC ACATATGGAA GGATCACCAA GGATGTAGTT
    CTACAGTGGG GAGAGATGCC TACATCAGTA GCATATATTC GATCCAATCA GACAATGGGC
    TGGGGAGAGA AGGCCATAGA GATCCGATCT GTGGAAACTG GTCACTTGGA TGGTGTGTTC
    ATGCACAAAA GGGCTCAAAG ACTAAAATTC TTGTGTGAAC GCAATGACAA GGTGTTCTTT
    GCCTCTGTTC GGTCTGGTGG CAGCAGTCAG GTTTATTTCA TGACCTTAGG CAGGACTTCT
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36828
    Clone ID Related Accession (Same CDS sequence) XM_005264050.3
    Accession Version XM_005264050.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264050.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT
    GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC
    CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT
    GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC
    GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA
    GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC
    AGCAGTATTG AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC
    AGTGGCAGCT CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT
    CAGAGTGGCT CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA
    TCTCAGCGCC TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC
    CTCAAGCCTG CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA
    GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG
    ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA
    GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA
    ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC
    ACTCTAATCG TCCGCCAGAG TACAGTTGAC CAAAAGCGTG CCAGCCATCA TGAGAGCAAT
    GGCTTTGCCG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC
    ACTTCCTCCA CCTCCTCCTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG
    ACACCCCAGG ACAAGCTCAC TGCTAATGAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA
    TCTAGCGGAA CAACAGTGAC ATCTGTGGTG GGATTTTCCT GTGATGGGAT GAGACCAGAA
    GCCATAAGGC AAGATCCTAC CCGGAAAGGC TCAGTGGTCA ATGTGAATCC TACCAACACT
    AGGCCACAGA GTGACACCCC GGAGATTCGT AAATACAAGA AGAGGTTTAA CTCTGAGATT
    CTGTGTGCTG CCTTATGGGG AGTGAATTTG CTAGTGGGTA CAGAGAGTGG CCTGATGCTG
    CTGGACAGAA GTGGCCAAGG GAAGGTCTAT CCTCTTATCA ACCGAAGACG ATTTCAACAA
    ATGGACGTAC TTGAGGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAATGATCC AGAAGTTGAG
    AAGAAGCAGG GATGGACAAC CGTAGGGGAT TTGGAAGGAT GTGTACATTA TAAAGTTGTA
    AAATATGAAA GAATCAAATT TCTGGTGATT GCTTTGAAGA GTTCTGTGGA AGTCTATGCG
    TGGGCACCAA AGCCATATCA CAAATTTATG GCCTTTAAGT CATTTGGAGA ATTGGTACAT
    AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GATTCAGGAT CAGTCTATGA CATTTATCTA
    CCAACACATG TAAGAAAGAA CCCACACTCT ATGATCCAGT GTAGCATCAA ACCCCATGCA
    ATCATCATCC TCCCCAATAC AGATGGAATG GAGCTTCTGG TGTGCTATGA AGATGAGGGG
    GTTTATGTAA ACACATATGG AAGGATCACC AAGGATGTAG TTCTACAGTG GGGAGAGATG
    CCTACATCAG TAGCATATAT TCGATCCAAT CAGACAATGG GCTGGGGAGA GAAGGCCATA
    GAGATCCGAT CTGTGGAAAC TGGTCACTTG GATGGTGTGT TCATGCACAA AAGGGCTCAA
    AGACTAAAAT TCTTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT
    GGCAGCAGTC AGGTTTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264107.1
    CDS75..4091
    Translation

    Target ORF information:

    RefSeq Version XM_005264050.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264050.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT
    GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC
    CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT
    GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC
    GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA
    GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC
    AGCAGTATTG AGCCCAGGCT TCTGTGGGAG AGAGTGGAGA AGCTGGTGCC CAGACCTGGC
    AGTGGCAGCT CCTCAGGGTC CAGCAACTCA GGATCCCAGC CCGGGTCTCA CCCTGGGTCT
    CAGAGTGGCT CCGGGGAACG CTTCAGAGTG AGATCATCAT CCAAGTCTGA AGGCTCTCCA
    TCTCAGCGCC TGGAAAATGC AGTGAAAAAA CCTGAAGATA AAAAGGAAGT TTTCAGACCC
    CTCAAGCCTG CTGGCGAAGT GGATCTGACC GCACTGGCCA AAGAGCTTCG AGCAGTGGAA
    GATGTACGGC CACCTCACAA AGTAACGGAC TACTCCTCAT CCAGTGAGGA GTCGGGGACG
    ACGGATGAGG AGGACGACGA TGTGGAGCAG GAAGGGGCTG ACGAGTCCAC CTCAGGACCA
    GAGGACACCA GAGCAGCGTC ATCTCTGAAT TTGAGCAATG GTGAAACGGA ATCTGTGAAA
    ACCATGATTG TCCATGATGA TGTAGAAAGT GAGCCGGCCA TGACCCCATC CAAGGAGGGC
    ACTCTAATCG TCCGCCAGAG TACAGTTGAC CAAAAGCGTG CCAGCCATCA TGAGAGCAAT
    GGCTTTGCCG GTCGCATTCA CCTCTTGCCA GATCTCTTAC AGCAAAGCCA TTCCTCCTCC
    ACTTCCTCCA CCTCCTCCTC CCCATCCTCC AGCCAGCCGA CACCCACCAT GTCCCCACAG
    ACACCCCAGG ACAAGCTCAC TGCTAATGAG ACTCAGTCCG CTAGTAGCAC ACTCCAGAAA
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA
    TCTAGCGGAA CAACAGTGAC ATCTGTGGTG GGATTTTCCT GTGATGGGAT GAGACCAGAA
    GCCATAAGGC AAGATCCTAC CCGGAAAGGC TCAGTGGTCA ATGTGAATCC TACCAACACT
    AGGCCACAGA GTGACACCCC GGAGATTCGT AAATACAAGA AGAGGTTTAA CTCTGAGATT
    CTGTGTGCTG CCTTATGGGG AGTGAATTTG CTAGTGGGTA CAGAGAGTGG CCTGATGCTG
    CTGGACAGAA GTGGCCAAGG GAAGGTCTAT CCTCTTATCA ACCGAAGACG ATTTCAACAA
    ATGGACGTAC TTGAGGGCTT GAATGTCTTG GTGACAATAT CTGGCAAAAA GGATAAGTTA
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAATGATCC AGAAGTTGAG
    AAGAAGCAGG GATGGACAAC CGTAGGGGAT TTGGAAGGAT GTGTACATTA TAAAGTTGTA
    AAATATGAAA GAATCAAATT TCTGGTGATT GCTTTGAAGA GTTCTGTGGA AGTCTATGCG
    TGGGCACCAA AGCCATATCA CAAATTTATG GCCTTTAAGT CATTTGGAGA ATTGGTACAT
    AAGCCATTAC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGTTGAAAGT GATCTATGGA
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GATTCAGGAT CAGTCTATGA CATTTATCTA
    CCAACACATG TAAGAAAGAA CCCACACTCT ATGATCCAGT GTAGCATCAA ACCCCATGCA
    ATCATCATCC TCCCCAATAC AGATGGAATG GAGCTTCTGG TGTGCTATGA AGATGAGGGG
    GTTTATGTAA ACACATATGG AAGGATCACC AAGGATGTAG TTCTACAGTG GGGAGAGATG
    CCTACATCAG TAGCATATAT TCGATCCAAT CAGACAATGG GCTGGGGAGA GAAGGCCATA
    GAGATCCGAT CTGTGGAAAC TGGTCACTTG GATGGTGTGT TCATGCACAA AAGGGCTCAA
    AGACTAAAAT TCTTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCTGT TCGGTCTGGT
    GGCAGCAGTC AGGTTTATTT CATGACCTTA GGCAGGACTT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36829
    Clone ID Related Accession (Same CDS sequence) XM_005264052.3
    Accession Version XM_005264052.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005264052.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT
    GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC
    CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT
    GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC
    GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA
    GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC
    AGTATTGAGC CCAGGCTTCT GTGGGAGAGA GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT
    GGCAGCTCCT CAGGGTCCAG CAACTCAGGA TCCCAGCCCG GGTCTCACCC TGGGTCTCAG
    AGTGGCTCCG GGGAACGCTT CAGAGTGAGA TCATCATCCA AGTCTGAAGG CTCTCCATCT
    CAGCGCCTGG AAAATGCAGT GAAAAAACCT GAAGATAAAA AGGAAGTTTT CAGACCCCTC
    AAGCCTGCTG GCGAAGTGGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG
    GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG
    GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC
    ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT
    CTAATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC
    TTTGCCGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CCTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT
    AGCGGAACAA CAGTGACATC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG
    CCACAGAGTG ACACCCCGGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAGATTCTG
    TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG
    GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTTATCAACC GAAGACGATT TCAACAAATG
    GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG
    AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA
    TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCGTGG
    GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG
    CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCAGGATCAG TCTATGACAT TTATCTACCA
    ACACATGTAA GAAAGAACCC ACACTCTATG ATCCAGTGTA GCATCAAACC CCATGCAATC
    ATCATCCTCC CCAATACAGA TGGAATGGAG CTTCTGGTGT GCTATGAAGA TGAGGGGGTT
    TATGTAAACA CATATGGAAG GATCACCAAG GATGTAGTTC TACAGTGGGG AGAGATGCCT
    ACATCAGTAG CATATATTCG ATCCAATCAG ACAATGGGCT GGGGAGAGAA GGCCATAGAG
    ATCCGATCTG TGGAAACTGG TCACTTGGAT GGTGTGTTCA TGCACAAAAG GGCTCAAAGA
    CTAAAATTCT TGTGTGAACG CAATGACAAG GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC
    AGCAGTCAGG TTTATTTCAT GACCTTAGGC AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264109.1
    CDS75..4088
    Translation

    Target ORF information:

    RefSeq Version XM_005264052.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264052.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC AAAAAGTCTG GTGGACATCG ACCTCTCCTC CCTGCGGGAT 
    CCTGCTGGGA TTTTTGAGCT GGTGGAAGTG GTTGGAAATG GCACCTATGG ACAAGTCTAT
    AAGGGTCGAC ATGTTAAAAC GGGTCAGTTG GCAGCCATCA AAGTTATGGA TGTCACTGAG
    GATGAAGAGG AAGAAATCAA ACTGGAGATA AATATGCTAA AGAAATACTC TCATCACAGA
    AACATTGCAA CATATTATGG TGCTTTCATC AAAAAGAGCC CTCCAGGACA TGATGACCAA
    CTCTGGCTTG TTATGGAGTT CTGTGGGGCT GGGTCCATTA CAGACCTTGT GAAGAACACC
    AAAGGGAACA CACTCAAAGA AGACTGGATC GCTTACATCT CCAGAGAAAT CCTGAGGGGA
    CTGGCACATC TTCACATTCA TCATGTGATT CACCGGGATA TCAAGGGCCA GAATGTGTTG
    CTGACTGAGA ATGCAGAGGT GAAACTTGTT GACTTTGGTG TGAGTGCTCA GCTGGACAGG
    ACTGTGGGGC GGAGAAATAC GTTCATAGGC ACTCCCTACT GGATGGCTCC TGAGGTCATC
    GCCTGTGATG AGAACCCAGA TGCCACCTAT GATTACAGAA GTGATCTTTG GTCTTGTGGC
    ATTACAGCCA TTGAGATGGC AGAAGGTGCT CCCCCTCTCT GTGACATGCA TCCAATGAGA
    GCACTGTTTC TCATTCCCAG AAACCCTCCT CCCCGGCTGA AGTCAAAAAA ATGGTCGAAG
    AAGTTTTTTA GTTTTATAGA AGGGTGCCTG GTGAAGAATT ACATGCAGCG GCCCTCTACA
    GAGCAGCTTT TGAAACATCC TTTTATAAGG GATCAGCCAA ATGAAAGGCA AGTTAGAATC
    CAGCTTAAGG ATCATATAGA TCGTACCAGG AAGAAGAGAG GCGAGAAAGA TGAAACTGAG
    TATGAGTACA GTGGGAGTGA GGAAGAAGAG GAGGAAGTGC CTGAACAGGA AGGAGAGCCA
    AGTTCCATTG TGAACGTGCC TGGTGAGTCT ACTCTTCGCC GAGATTTCCT GAGACTGCAG
    CAGGAGAACA AGGAACGTTC CGAGGCTCTT CGGAGACAAC AGTTACTACA GGAGCAACAG
    CTCCGGGAGC AGGAAGAATA TAAAAGGCAA CTGCTGGCAG AGAGACAGAA GCGGATTGAG
    CAGCAGAAAG AACAGAGGCG ACGGCTAGAA GAGCAACAAA GGAGAGAGCG GGAAGCTAGA
    AGGCAGCAGG AACGTGAACA GCGAAGGAGA GAACAAGAAG AAAAGAGGCG TCTAGAGGAG
    TTGGAGAGAA GGCGCAAAGA AGAAGAGGAG AGGAGACGGG CAGAAGAAGA AAAGAGGAGA
    GTTGAAAGAG AACAGGAGTA TATCAGGCGA CAGCTAGAAG AGGAGCAGCG GCACTTGGAA
    GTCCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGTTAC TGGAGTGCCG ATGGCGGGAG
    ATGGAGGAGC ACCGGCAGGC AGAGAGGCTC CAGAGGCAGT TGCAACAAGA ACAAGCATAT
    CTCCTGTCTC TACAGCATGA CCATAGGAGG CCGCACCCGC AGCACTCGCA GCAGCCGCCA
    CCACCGCAGC AGGAAAGGAG CAAGCCAAGC TTCCATGCTC CCGAGCCCAA AGCCCACTAC
    GAGCCTGCTG ACCGAGCGCG AGAGGTACAG TGGTCCCACC TGGCATCTCT CAAGAACAAT
    GTTTCCCCTG TCTCGCGATC CCATTCCTTC AGTGACCCTT CTCCCAAATT TGCACACCAC
    CATCTTCGTT CTCAGGACCC ATGTCCACCT TCCCGCAGTG AGGTGCTCAG TCAGAGCTCT
    GACTCTAAGT CAGAGGCGCC TGACCCTACC CAAAAGGCTT GGTCTAGATC AGACAGTGAC
    GAGGTGCCTC CAAGGGTTCC TGTGAGAACA ACATCTCGCT CCCCTGTTCT GTCCCGTCGA
    GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AATAGCCAGG CAGGACAGAG AAACTCCACC
    AGTATTGAGC CCAGGCTTCT GTGGGAGAGA GTGGAGAAGC TGGTGCCCAG ACCTGGCAGT
    GGCAGCTCCT CAGGGTCCAG CAACTCAGGA TCCCAGCCCG GGTCTCACCC TGGGTCTCAG
    AGTGGCTCCG GGGAACGCTT CAGAGTGAGA TCATCATCCA AGTCTGAAGG CTCTCCATCT
    CAGCGCCTGG AAAATGCAGT GAAAAAACCT GAAGATAAAA AGGAAGTTTT CAGACCCCTC
    AAGCCTGCTG GCGAAGTGGA TCTGACCGCA CTGGCCAAAG AGCTTCGAGC AGTGGAAGAT
    GTACGGCCAC CTCACAAAGT AACGGACTAC TCCTCATCCA GTGAGGAGTC GGGGACGACG
    GATGAGGAGG ACGACGATGT GGAGCAGGAA GGGGCTGACG AGTCCACCTC AGGACCAGAG
    GACACCAGAG CAGCGTCATC TCTGAATTTG AGCAATGGTG AAACGGAATC TGTGAAAACC
    ATGATTGTCC ATGATGATGT AGAAAGTGAG CCGGCCATGA CCCCATCCAA GGAGGGCACT
    CTAATCGTCC GCCAGAGTAC AGTTGACCAA AAGCGTGCCA GCCATCATGA GAGCAATGGC
    TTTGCCGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT
    TCCTCCACCT CCTCCTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA
    CCCCAGGACA AGCTCACTGC TAATGAGACT CAGTCCGCTA GTAGCACACT CCAGAAACAC
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT
    AGCGGAACAA CAGTGACATC TGTGGTGGGA TTTTCCTGTG ATGGGATGAG ACCAGAAGCC
    ATAAGGCAAG ATCCTACCCG GAAAGGCTCA GTGGTCAATG TGAATCCTAC CAACACTAGG
    CCACAGAGTG ACACCCCGGA GATTCGTAAA TACAAGAAGA GGTTTAACTC TGAGATTCTG
    TGTGCTGCCT TATGGGGAGT GAATTTGCTA GTGGGTACAG AGAGTGGCCT GATGCTGCTG
    GACAGAAGTG GCCAAGGGAA GGTCTATCCT CTTATCAACC GAAGACGATT TCAACAAATG
    GACGTACTTG AGGGCTTGAA TGTCTTGGTG ACAATATCTG GCAAAAAGGA TAAGTTACGT
    GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ATGATCCAGA AGTTGAGAAG
    AAGCAGGGAT GGACAACCGT AGGGGATTTG GAAGGATGTG TACATTATAA AGTTGTAAAA
    TATGAAAGAA TCAAATTTCT GGTGATTGCT TTGAAGAGTT CTGTGGAAGT CTATGCGTGG
    GCACCAAAGC CATATCACAA ATTTATGGCC TTTAAGTCAT TTGGAGAATT GGTACATAAG
    CCATTACTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGT TGAAAGTGAT CTATGGATCC
    TGTGCTGGAT TCCATGCTGT TGATGTGGAT TCAGGATCAG TCTATGACAT TTATCTACCA
    ACACATGTAA GAAAGAACCC ACACTCTATG ATCCAGTGTA GCATCAAACC CCATGCAATC
    ATCATCCTCC CCAATACAGA TGGAATGGAG CTTCTGGTGT GCTATGAAGA TGAGGGGGTT
    TATGTAAACA CATATGGAAG GATCACCAAG GATGTAGTTC TACAGTGGGG AGAGATGCCT
    ACATCAGTAG CATATATTCG ATCCAATCAG ACAATGGGCT GGGGAGAGAA GGCCATAGAG
    ATCCGATCTG TGGAAACTGG TCACTTGGAT GGTGTGTTCA TGCACAAAAG GGCTCAAAGA
    CTAAAATTCT TGTGTGAACG CAATGACAAG GTGTTCTTTG CCTCTGTTCG GTCTGGTGGC
    AGCAGTCAGG TTTATTTCAT GACCTTAGGC AGGACTTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.