Ddx10 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx10
Entrez Gene ID	300710
Full Name	DEAD-box helicase 10
General protein information	Preferred Names DEAD-box helicase 10 Names probable ATP-dependent RNA helicase DDX10 DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106820.1	NP_001100290.1	probable ATP-dependent RNA helicase DDX10

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DDX10	XP_001141618.1
Canis lupus familiaris (dog)	DDX10	XP_536583.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001057	XP_001230842.3
Mus musculus (house mouse)	Ddx10	NP_084212.2
Drosophila melanogaster (fruit fly)	CG5800	NP_573230.1
Caenorhabditis elegans (roundworm)	Y23H5B.6	NP_490989.1
Danio rerio (zebrafish)	ddx10	XP_001922220.1
Homo sapiens (human)	DDX10	NP_004389.2
Rattus norvegicus (Norway rat)	Ddx10	NP_001100290.1
Xenopus tropicalis (tropical clawed frog)	ddx10	NP_001096151.1
Bos taurus (cattle)	DDX10	NP_001070349.1
Gallus gallus (chicken)	DDX10	NP_001073193.1

Gene Ontology
Bibliography

Related articles in PubMed

Discovery and characterization of spontaneous mouse models of craniofacial dysmorphology.
Palmer K, Fairfield H, Borgeia S, Curtain M, Hassan MG, Dionne L, Yong Karst S, Coombs H, Bronson RT, Reinholdt LG, Bergstrom DE, Donahue LR, Cox TC, Murray SA
Developmental biology415(2)216-227(2016 07)

DExD/H-box RNA helicases in ribosome biogenesis.
Martin R, Straub AU, Doebele C, Bohnsack MT
RNA biology10(1)4-18(2013 Jan)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ddx10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05000	NM_001106820.1 Latest version!	Rattus norvegicus DEAD-box helicase 10 (Ddx10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx10 ( NM_001106820.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100290.1 Rattus norvegicus DEAD-box helicase 10 (Ddx10), mRNA.

Price & Availability↑

CloneID	ORa05000
Clone ID Related Accession (Same CDS sequence)	NM_001106820.1
Accession Version	NM_001106820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-13
Organism	Rattus norvegicus(Norway rat)
Product	probable ATP-dependent RNA helicase DDX10
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473975.1. On or before Oct 4, 2007 this sequence version replaced XM_236263.4, XM_001071986.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGCAAAA CGGTTGGTTC TCTGGGTCAG GGAACCCGAC CGGACCCGGT GAGGAGCTTC 
AACCGCTGGA AGAAAAAACA CAGCCACAGG CAGCACCAAA AGAAGGAACG GAGGAAGCAG 
CTTAAGAAGC CGGAATGGCA GGTCGAACGT GAGGGTATCA GCCGCCTCAT GCAAAATTAC 
GAGAAGATAA ATGTAAATGA AATTACAAGA TTTTCAGATT TCCCTTTATC CAAAAAAACG 
TTGAAAGGTT TGCAAGAAGC GCAATACCGC TTGGTAACAG AGATACAGAA GCAGACCATA 
GGGTTGGCTT TGCAAGGTAA AGATGTGCTT GGAGCTGCCA AAACTGGGTC TGGCAAGACT 
TTGGCTTTTC TCGTTCCAGT ATTGGAAGCC TTATACCGCC TGCAGTGGAC TTCAACAGAC 
GGCCTGGGAG TTCTGATAAT ATCACCTACT CGAGAACTGG CCTATCAGAC ATTTGAGGTT 
CTACGCAAAG TAGGAAAGAA CCATGATTTT TCAGCTGGTC TCATCATTGG TGGGAAGGAT 
CTAAAACATG AAGCTGAGAG GATCAACAAC ATAAATATAC TCGTTTGCAC ACCAGGTCGA 
CTTCTTCAAC ACATGGATGA AACAATCTGT TTTCATGCCA CCAATCTTCA GATGTTAGTT 
CTTGATGAGG CAGACAGAAT CTTAGATATG GGCTTTGCTG ATACCATGAA TGCTATCATT 
GAAAATCTTC CCAAGAAACG CCAGACTTTG CTGTTCTCAG CTACACAGAC CAAATCTGTG 
AAGGACCTTG CGCGCTTGAG TTTGAAAGAC CCTGAGTATG TCTGGGTTCA TGAGAAAGCA 
AAATATAGCA CCCCTGCTAC ATTGGAACAG AACTATATAA TTTGTGAGCT GCACCAAAAA 
ATAAGTGTGC TGTTTTCATT TTTGAGAAGC CACCTAACGA AGAAGAGTAT TGTGTTTTTC 
TCCAGTTGCA AAGAGGTGCA GTATCTGTAC AGGGTGTTCT GCCGGCTGCG CCCAGGGATC 
TCTATCCTTG CTCTCCATGG CCGGCAGCAG CAGATGCGAA GAATGGAAGT CTACAATGAA 
TTTGTCCGAA AGAGGGCTGC AGTCCTTTTT GCTACTGATA TTGCAGCCAG GGGATTGGAT 
TTTCCAGCTG TGAATTGGGT CCTTCAGTTT GATTGTCCAG AGGATGCCAA CACGTATATT 
CACAGAGCTG GTAGAACTGC CAGGTACAAG GAGGACGGAG AAGCGTTATT AATTTTACTG 
CCCTCCGAAG AACAAGGCAT GGTGCAGCAG CTTCTCCAGA AGAAAGTTCC TGTGAAGGAG 
ATCAAAATCA ATCCAGAAAA ACTTATCGAT GTTCAGAAAA GATTAGAATC ATTTTTAGCT 
CAAGATCAAG ATTTAAAAGA AAGAGCTCAA AGGTGTTTTG TCTCCTACAT ACGTTCAGTA 
TATCTTATGA AGGATAAAGA AGTATTTGAT GTGAACAAGT TACCTATTAC TGAATATGCA 
CTGTCTCTTG GTCTTGCTGT GGCACCACGG ATAAGATTTC TTCAGAAAAT GGAGAAACAG 
CCCAACAAAG AATTGGGAAA GAATCAAGTC ACTGAAGCAA TTCAGCCCAG GGCTCCCTCC 
CTCACCAGTG ATGAAGTAGA AGAATTTCGA GCTTATTTCA GTGAGAAAAT GTCCATCCTT 
CACAAAAGTG GGAAAAGACT AGAAGAGACA GAACACAGCT TGGCTAGTGG TGAGAGTGAA 
GAACAGGATG AAGAGACGGA AGATGAAGAA ATGGAAGATC ATGTGGGACA AACAAGAGAG 
CCTCCCACTG ACTCTGTTCC CAGGAGTGAG GAGGCACAGA AGGTCAAGGA AGTTCCTGTG 
CAGTTCTTAG ACAGGGATGA TGATGAGGAA GATGGGCCTG ATGCTGACTT CCTCACAGTG 
AAGCGGCGTG ATGTGTTTGG GTTGGATCTT AAAGAGAATG AAGCACTGTC GAAAAAGGAA 
CCTTCTAAAT CCAGTGTCAA GAAAAAATTA ACCAAAGTTG CAGAAGCAAA AAAAGTAATG 
AAGAGAAATT TTAAAGTGAA CAAGAAAATA ACATTTACGG ATGAAGGAGA GTTGGTTCAG 
CAGTGGCCAC AGATTCAGAA ATGTGCTACA AATGATGTGG AGGAAGAAGA TGACACTGGT 
GGCATCAACC TAGACAAAGC CAAAGAAAGG CTTCAGGAAG AGGACAAATT TGACAAAGAA 
GAATATAGGA AGAAAATTAA GGCAAAGCAT CGGGAAAGAC GCTTAAAAGA GAGAGAGGCC 
AGAAGAGAAG CCAACAAAAG ACAAGCAAAG GCCAGAGATG AAGAGGAAGC CTTTCTGGAC 
TGGAGTGATG AAGATGATGG TGGATTTGAT CCAAGCACAC TTCCAGATCC AGATAAGCAC 
AGAAGTTCTG AAGAATCAGA GAGTGAAGAT ACAAAACACA AGATGAGTGA CACCAAGAAG 
AAGCAGGAAA TGAGGAAGAG AAACAACACT GAAGTCGATG ATGTAAGACC AAGAAGTCGT 
CATGGAAAGA AGGCCAAGTG GGAAACGTTA GAACCTTTGG ATACAGGCCT GTCTTTAGCA 
GAGGACGAAG AGCTGGTGTT ACATCTCCTC AAAAGTCAAA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100290.1
CDS	137..2761
Translation	MGKTVGSLGQGTRPDPVRSFNRWKKKHSHRQHQKKERRKQLKKPEWQVEREGISRLMQNYEKINVNEITRFSDFPLSKKTLKGLQEAQYRLVTEIQKQTIGLALQGKDVLGAAKTGSGKTLAFLVPVLEALYRLQWTSTDGLGVLIISPTRELAYQTFEVLRKVGKNHDFSAGLIIGGKDLKHEAERINNINILVCTPGRLLQHMDETICFHATNLQMLVLDEADRILDMGFADTMNAIIENLPKKRQTLLFSATQTKSVKDLARLSLKDPEYVWVHEKAKYSTPATLEQNYIICELHQKISVLFSFLRSHLTKKSIVFFSSCKEVQYLYRVFCRLRPGISILALHGRQQQMRRMEVYNEFVRKRAAVLFATDIAARGLDFPAVNWVLQFDCPEDANTYIHRAGRTARYKEDGEALLILLPSEEQGMVQQLLQKKVPVKEIKINPEKLIDVQKRLESFLAQDQDLKERAQRCFVSYIRSVYLMKDKEVFDVNKLPITEYALSLGLAVAPRIRFLQKMEKQPNKELGKNQVTEAIQPRAPSLTSDEVEEFRAYFSEKMSILHKSGKRLEETEHSLASGESEEQDEETEDEEMEDHVGQTREPPTDSVPRSEEAQKVKEVPVQFLDRDDDEEDGPDADFLTVKRRDVFGLDLKENEALSKKEPSKSSVKKKLTKVAEAKKVMKRNFKVNKKITFTDEGELVQQWPQIQKCATNDVEEEDDTGGINLDKAKERLQEEDKFDKEEYRKKIKAKHRERRLKEREARREANKRQAKARDEEEAFLDWSDEDDGGFDPSTLPDPDKHRSSEESESEDTKHKMSDTKKKQEMRKRNNTEVDDVRPRSRHGKKAKWETLEPLDTGLSLAEDEELVLHLLKSQN

Target ORF information:

RefSeq Version	NM_001106820.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAD-box helicase 10 (Ddx10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106820.1

ORF Insert Sequence:

ATGGGCAAAA CGGTTGGTTC TCTGGGTCAG GGAACCCGAC CGGACCCGGT GAGGAGCTTC 
AACCGCTGGA AGAAAAAACA CAGCCACAGG CAGCACCAAA AGAAGGAACG GAGGAAGCAG 
CTTAAGAAGC CGGAATGGCA GGTCGAACGT GAGGGTATCA GCCGCCTCAT GCAAAATTAC 
GAGAAGATAA ATGTAAATGA AATTACAAGA TTTTCAGATT TCCCTTTATC CAAAAAAACG 
TTGAAAGGTT TGCAAGAAGC GCAATACCGC TTGGTAACAG AGATACAGAA GCAGACCATA 
GGGTTGGCTT TGCAAGGTAA AGATGTGCTT GGAGCTGCCA AAACTGGGTC TGGCAAGACT 
TTGGCTTTTC TCGTTCCAGT ATTGGAAGCC TTATACCGCC TGCAGTGGAC TTCAACAGAC 
GGCCTGGGAG TTCTGATAAT ATCACCTACT CGAGAACTGG CCTATCAGAC ATTTGAGGTT 
CTACGCAAAG TAGGAAAGAA CCATGATTTT TCAGCTGGTC TCATCATTGG TGGGAAGGAT 
CTAAAACATG AAGCTGAGAG GATCAACAAC ATAAATATAC TCGTTTGCAC ACCAGGTCGA 
CTTCTTCAAC ACATGGATGA AACAATCTGT TTTCATGCCA CCAATCTTCA GATGTTAGTT 
CTTGATGAGG CAGACAGAAT CTTAGATATG GGCTTTGCTG ATACCATGAA TGCTATCATT 
GAAAATCTTC CCAAGAAACG CCAGACTTTG CTGTTCTCAG CTACACAGAC CAAATCTGTG 
AAGGACCTTG CGCGCTTGAG TTTGAAAGAC CCTGAGTATG TCTGGGTTCA TGAGAAAGCA 
AAATATAGCA CCCCTGCTAC ATTGGAACAG AACTATATAA TTTGTGAGCT GCACCAAAAA 
ATAAGTGTGC TGTTTTCATT TTTGAGAAGC CACCTAACGA AGAAGAGTAT TGTGTTTTTC 
TCCAGTTGCA AAGAGGTGCA GTATCTGTAC AGGGTGTTCT GCCGGCTGCG CCCAGGGATC 
TCTATCCTTG CTCTCCATGG CCGGCAGCAG CAGATGCGAA GAATGGAAGT CTACAATGAA 
TTTGTCCGAA AGAGGGCTGC AGTCCTTTTT GCTACTGATA TTGCAGCCAG GGGATTGGAT 
TTTCCAGCTG TGAATTGGGT CCTTCAGTTT GATTGTCCAG AGGATGCCAA CACGTATATT 
CACAGAGCTG GTAGAACTGC CAGGTACAAG GAGGACGGAG AAGCGTTATT AATTTTACTG 
CCCTCCGAAG AACAAGGCAT GGTGCAGCAG CTTCTCCAGA AGAAAGTTCC TGTGAAGGAG 
ATCAAAATCA ATCCAGAAAA ACTTATCGAT GTTCAGAAAA GATTAGAATC ATTTTTAGCT 
CAAGATCAAG ATTTAAAAGA AAGAGCTCAA AGGTGTTTTG TCTCCTACAT ACGTTCAGTA 
TATCTTATGA AGGATAAAGA AGTATTTGAT GTGAACAAGT TACCTATTAC TGAATATGCA 
CTGTCTCTTG GTCTTGCTGT GGCACCACGG ATAAGATTTC TTCAGAAAAT GGAGAAACAG 
CCCAACAAAG AATTGGGAAA GAATCAAGTC ACTGAAGCAA TTCAGCCCAG GGCTCCCTCC 
CTCACCAGTG ATGAAGTAGA AGAATTTCGA GCTTATTTCA GTGAGAAAAT GTCCATCCTT 
CACAAAAGTG GGAAAAGACT AGAAGAGACA GAACACAGCT TGGCTAGTGG TGAGAGTGAA 
GAACAGGATG AAGAGACGGA AGATGAAGAA ATGGAAGATC ATGTGGGACA AACAAGAGAG 
CCTCCCACTG ACTCTGTTCC CAGGAGTGAG GAGGCACAGA AGGTCAAGGA AGTTCCTGTG 
CAGTTCTTAG ACAGGGATGA TGATGAGGAA GATGGGCCTG ATGCTGACTT CCTCACAGTG 
AAGCGGCGTG ATGTGTTTGG GTTGGATCTT AAAGAGAATG AAGCACTGTC GAAAAAGGAA 
CCTTCTAAAT CCAGTGTCAA GAAAAAATTA ACCAAAGTTG CAGAAGCAAA AAAAGTAATG 
AAGAGAAATT TTAAAGTGAA CAAGAAAATA ACATTTACGG ATGAAGGAGA GTTGGTTCAG 
CAGTGGCCAC AGATTCAGAA ATGTGCTACA AATGATGTGG AGGAAGAAGA TGACACTGGT 
GGCATCAACC TAGACAAAGC CAAAGAAAGG CTTCAGGAAG AGGACAAATT TGACAAAGAA 
GAATATAGGA AGAAAATTAA GGCAAAGCAT CGGGAAAGAC GCTTAAAAGA GAGAGAGGCC 
AGAAGAGAAG CCAACAAAAG ACAAGCAAAG GCCAGAGATG AAGAGGAAGC CTTTCTGGAC 
TGGAGTGATG AAGATGATGG TGGATTTGAT CCAAGCACAC TTCCAGATCC AGATAAGCAC 
AGAAGTTCTG AAGAATCAGA GAGTGAAGAT ACAAAACACA AGATGAGTGA CACCAAGAAG 
AAGCAGGAAA TGAGGAAGAG AAACAACACT GAAGTCGATG ATGTAAGACC AAGAAGTCGT 
CATGGAAAGA AGGCCAAGTG GGAAACGTTA GAACCTTTGG ATACAGGCCT GTCTTTAGCA 
GAGGACGAAG AGCTGGTGTT ACATCTCCTC AAAAGTCAAA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Discovery and characterization of spontaneous mouse models of craniofacial dysmorphology.
Developmental biology415(2)216-227(2016 07)
Palmer K,Fairfield H,Borgeia S,Curtain M,Hassan MG,Dionne L,Yong Karst S,Coombs H,Bronson RT,Reinholdt LG,Bergstrom DE,Donahue LR,Cox TC,Murray SA

DExD/H-box RNA helicases in ribosome biogenesis.
RNA biology10(1)4-18(2013 Jan)
Martin R,Straub AU,Doebele C,Bohnsack MT

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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Ddx10 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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