Ddx10 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx10
Entrez Gene ID	77591
Full Name	DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
Synonyms	4632415A01Rik,AI646054
General protein information	Preferred Names DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 Names probable ATP-dependent RNA helicase DDX10 DEAD box protein 10 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 10 (RNA helicase)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A5.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006510684.4	XP_006510747.1	probable ATP-dependent RNA helicase DDX10 isoform X1
XM_006510684.3	XP_006510747.1	probable ATP-dependent RNA helicase DDX10 isoform X1
NM_029936.2	NP_084212.2	probable ATP-dependent RNA helicase DDX10

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DDX10	XP_001141618.1
Canis lupus familiaris (dog)	DDX10	XP_536583.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001057	XP_001230842.3
Mus musculus (house mouse)	Ddx10	NP_084212.2
Drosophila melanogaster (fruit fly)	CG5800	NP_573230.1
Caenorhabditis elegans (roundworm)	Y23H5B.6	NP_490989.1
Danio rerio (zebrafish)	ddx10	XP_001922220.1
Homo sapiens (human)	DDX10	NP_004389.2
Rattus norvegicus (Norway rat)	Ddx10	NP_001100290.1
Xenopus tropicalis (tropical clawed frog)	ddx10	NP_001096151.1
Bos taurus (cattle)	DDX10	NP_001070349.1
Gallus gallus (chicken)	DDX10	NP_001073193.1

Gene Ontology
Bibliography

Related articles in PubMed

Discovery and characterization of spontaneous mouse models of craniofacial dysmorphology.
Palmer K, Fairfield H, Borgeia S, Curtain M, Hassan MG, Dionne L, Yong Karst S, Coombs H, Bronson RT, Reinholdt LG, Bergstrom DE, Donahue LR, Cox TC, Murray SA
Developmental biology415(2)216-227(2016 07)

Exome sequencing reveals pathogenic mutations in 91 strains of mice with Mendelian disorders.
Fairfield H, Srivastava A, Ananda G, Liu R, Kircher M, Lakshminarayana A, Harris BS, Karst SY, Dionne LA, Kane CC, Curtain M, Berry ML, Ward-Bailey PF, Greenstein I, Byers C, Czechanski A, Sharp J, Palmer K, Gudis P, Martin W, Tadenev A, Bogdanik L, Pratt CH, Chang B, Schroeder DG, Cox GA, Cliften P, Milbrandt J, Murray S, Burgess R, Bergstrom DE, Donahue LR, Hamamy H, Masri A, Santoni FA, Makrythanasis P, Antonarakis SE, Shendure J, Reinholdt LG
Genome research25(7)948-57(2015 Jul)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ddx10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu42451	XM_006510684.4 Latest version!	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 (Ddx10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu15395	NM_029936.2 Latest version!	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 (Ddx10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
View Old Accession Versions >>
OMu42451	XM_006510684.3	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 (Ddx10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx10 ( XM_006510684.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510747.1 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 (Ddx10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu42451
Clone ID Related Accession (Same CDS sequence)	XM_006510684.4 , XM_006510684.3
Accession Version	XM_006510684.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent RNA helicase DDX10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510684.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAAAA CGGTTGCTTC TCTGGGTCAG GGAACCCGAC CCGACCCGGT GCGGAGCTTT 
AATCGCTGGA AGAAAAAACA CAGCCACAGG CAGCACCAAA AGAAGGAGCG GAGGAAGCAG 
CTAAAGAAGC CGGAATGGCA GGTCGAGCGC GAGGGGATCA GCCGCCTCAT GCAAAACTAC 
GAGAAGATAA ATGTAAATGA AATTACAAGA TTTTCAGATT TCCCTTTGTC CAAAAAAACA 
TTGAAAGGTT TGCAAGAAGC ACAGTACCGC TTGGTAACAG AGATACAGAA GCAGACCATA 
GGGTTGGCTT TGCAAGGTAA AGATGTGCTT GGTGCTGCCA AAACTGGATC TGGCAAGACT 
TTGGCTTTTC TTGTTCCGGT GTTGGAAGCC TTATACCGCC TGCAGTGGAC TTCAACAGAT 
GGCCTGGGAG TTCTGATAAT ATCACCTACT CGAGAACTGG CCTATCAGAC ATTTGAGGTT 
CTACGCAAAG TAGGGAAGAA TCATGATTTT TCAGCTGGTC TCATCATTGG TGGGAAGGAT 
CTGAAACATG AAGCTGAGAG GATCAACAAC ATAAACATAC TGGTTTGCAC ACCAGGTCGA 
CTTCTTCAAC ACATGGATGA AACAATATGT TTTCATGCCA CCAATCTTCA GATGTTAGTT 
CTTGATGAGG CAGATAGAAT CTTGGATATG GGCTTTGCTG ATACCATGAA TGCTATTATT 
GAAAATCTTC CCAAGAAACG CCAGACTTTA CTGTTCTCAG CCACACAGAC CAAATCTGTA 
AAGGACCTTG CGCGCTTGAG TTTGAAAGAC CCTGAGTATG TCTGGGTTCA TGAAAAAGCA 
AAATACAGCA CCCCTGCTAC GTTGGAACAG AACTATATAA TTTGTGAGCT GCACCAAAAA 
ATAAGTGTGC TGTTTTCCTT TTTGAGAAGC CACCTAAAGA AGAAGAGTAT TGTGTTTTTC 
TCCAGTTGCA AAGAGGTTCA GTATCTGTAT AGGGTGTTCT GCAGGCTGCG CCCAGGGATC 
TCGATCCTTG CTCTCCATGG CCGGCAGCAG CAGATGAGAA GAATGGAGGT CTACAATGAA 
TTTGTCCGAA AGAGGGCCGC AGTACTTTTT GCCACTGATA TTGCAGCTAG GGGACTGGAT 
TTTCCAGCTG TGAATTGGGT CCTTCAGTTT GATTGTCCAG AGGATGCCAA CACGTATATT 
CACAGAGCTG GTAGAACTGC CAGGTACAAA GAAGATGGAG AAGCGTTATT AATTTTACTA 
CCTTCAGAAG AGCAAGGAAT GGTGCAGCAG CTTCTCCAGA AGAAAGTACC TGTGAAGGAG 
ATCAACTATT TGGCCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510747.1
CDS	168..1508
Translation	MGKTVASLGQGTRPDPVRSFNRWKKKHSHRQHQKKERRKQLKKPEWQVEREGISRLMQNYEKINVNEITRFSDFPLSKKTLKGLQEAQYRLVTEIQKQTIGLALQGKDVLGAAKTGSGKTLAFLVPVLEALYRLQWTSTDGLGVLIISPTRELAYQTFEVLRKVGKNHDFSAGLIIGGKDLKHEAERINNINILVCTPGRLLQHMDETICFHATNLQMLVLDEADRILDMGFADTMNAIIENLPKKRQTLLFSATQTKSVKDLARLSLKDPEYVWVHEKAKYSTPATLEQNYIICELHQKISVLFSFLRSHLKKKSIVFFSSCKEVQYLYRVFCRLRPGISILALHGRQQQMRRMEVYNEFVRKRAAVLFATDIAARGLDFPAVNWVLQFDCPEDANTYIHRAGRTARYKEDGEALLILLPSEEQGMVQQLLQKKVPVKEINYLAI

Target ORF information:

RefSeq Version	XM_006510684.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 (Ddx10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510684.3

ORF Insert Sequence:

ATGGGCAAAA CGGTTGCTTC TCTGGGTCAG GGAACCCGAC CCGACCCGGT GCGGAGCTTT 
AATCGCTGGA AGAAAAAACA CAGCCACAGG CAGCACCAAA AGAAGGAGCG GAGGAAGCAG 
CTAAAGAAGC CGGAATGGCA GGTCGAGCGC GAGGGGATCA GCCGCCTCAT GCAAAACTAC 
GAGAAGATAA ATGTAAATGA AATTACAAGA TTTTCAGATT TCCCTTTGTC CAAAAAAACA 
TTGAAAGGTT TGCAAGAAGC ACAGTACCGC TTGGTAACAG AGATACAGAA GCAGACCATA 
GGGTTGGCTT TGCAAGGTAA AGATGTGCTT GGTGCTGCCA AAACTGGATC TGGCAAGACT 
TTGGCTTTTC TTGTTCCGGT GTTGGAAGCC TTATACCGCC TGCAGTGGAC TTCAACAGAT 
GGCCTGGGAG TTCTGATAAT ATCACCTACT CGAGAACTGG CCTATCAGAC ATTTGAGGTT 
CTACGCAAAG TAGGGAAGAA TCATGATTTT TCAGCTGGTC TCATCATTGG TGGGAAGGAT 
CTGAAACATG AAGCTGAGAG GATCAACAAC ATAAACATAC TGGTTTGCAC ACCAGGTCGA 
CTTCTTCAAC ACATGGATGA AACAATATGT TTTCATGCCA CCAATCTTCA GATGTTAGTT 
CTTGATGAGG CAGATAGAAT CTTGGATATG GGCTTTGCTG ATACCATGAA TGCTATTATT 
GAAAATCTTC CCAAGAAACG CCAGACTTTA CTGTTCTCAG CCACACAGAC CAAATCTGTA 
AAGGACCTTG CGCGCTTGAG TTTGAAAGAC CCTGAGTATG TCTGGGTTCA TGAAAAAGCA 
AAATACAGCA CCCCTGCTAC GTTGGAACAG AACTATATAA TTTGTGAGCT GCACCAAAAA 
ATAAGTGTGC TGTTTTCCTT TTTGAGAAGC CACCTAAAGA AGAAGAGTAT TGTGTTTTTC 
TCCAGTTGCA AAGAGGTTCA GTATCTGTAT AGGGTGTTCT GCAGGCTGCG CCCAGGGATC 
TCGATCCTTG CTCTCCATGG CCGGCAGCAG CAGATGAGAA GAATGGAGGT CTACAATGAA 
TTTGTCCGAA AGAGGGCCGC AGTACTTTTT GCCACTGATA TTGCAGCTAG GGGACTGGAT 
TTTCCAGCTG TGAATTGGGT CCTTCAGTTT GATTGTCCAG AGGATGCCAA CACGTATATT 
CACAGAGCTG GTAGAACTGC CAGGTACAAA GAAGATGGAG AAGCGTTATT AATTTTACTA 
CCTTCAGAAG AGCAAGGAAT GGTGCAGCAG CTTCTCCAGA AGAAAGTACC TGTGAAGGAG 
ATCAACTATT TGGCCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx10 ( XM_006510684.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006510747.1 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 (Ddx10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu42451
Clone ID Related Accession (Same CDS sequence)	XM_006510684.4 , XM_006510684.3
Accession Version	XM_006510684.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent RNA helicase DDX10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006510684.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAAAA CGGTTGCTTC TCTGGGTCAG GGAACCCGAC CCGACCCGGT GCGGAGCTTT 
AATCGCTGGA AGAAAAAACA CAGCCACAGG CAGCACCAAA AGAAGGAGCG GAGGAAGCAG 
CTAAAGAAGC CGGAATGGCA GGTCGAGCGC GAGGGGATCA GCCGCCTCAT GCAAAACTAC 
GAGAAGATAA ATGTAAATGA AATTACAAGA TTTTCAGATT TCCCTTTGTC CAAAAAAACA 
TTGAAAGGTT TGCAAGAAGC ACAGTACCGC TTGGTAACAG AGATACAGAA GCAGACCATA 
GGGTTGGCTT TGCAAGGTAA AGATGTGCTT GGTGCTGCCA AAACTGGATC TGGCAAGACT 
TTGGCTTTTC TTGTTCCGGT GTTGGAAGCC TTATACCGCC TGCAGTGGAC TTCAACAGAT 
GGCCTGGGAG TTCTGATAAT ATCACCTACT CGAGAACTGG CCTATCAGAC ATTTGAGGTT 
CTACGCAAAG TAGGGAAGAA TCATGATTTT TCAGCTGGTC TCATCATTGG TGGGAAGGAT 
CTGAAACATG AAGCTGAGAG GATCAACAAC ATAAACATAC TGGTTTGCAC ACCAGGTCGA 
CTTCTTCAAC ACATGGATGA AACAATATGT TTTCATGCCA CCAATCTTCA GATGTTAGTT 
CTTGATGAGG CAGATAGAAT CTTGGATATG GGCTTTGCTG ATACCATGAA TGCTATTATT 
GAAAATCTTC CCAAGAAACG CCAGACTTTA CTGTTCTCAG CCACACAGAC CAAATCTGTA 
AAGGACCTTG CGCGCTTGAG TTTGAAAGAC CCTGAGTATG TCTGGGTTCA TGAAAAAGCA 
AAATACAGCA CCCCTGCTAC GTTGGAACAG AACTATATAA TTTGTGAGCT GCACCAAAAA 
ATAAGTGTGC TGTTTTCCTT TTTGAGAAGC CACCTAAAGA AGAAGAGTAT TGTGTTTTTC 
TCCAGTTGCA AAGAGGTTCA GTATCTGTAT AGGGTGTTCT GCAGGCTGCG CCCAGGGATC 
TCGATCCTTG CTCTCCATGG CCGGCAGCAG CAGATGAGAA GAATGGAGGT CTACAATGAA 
TTTGTCCGAA AGAGGGCCGC AGTACTTTTT GCCACTGATA TTGCAGCTAG GGGACTGGAT 
TTTCCAGCTG TGAATTGGGT CCTTCAGTTT GATTGTCCAG AGGATGCCAA CACGTATATT 
CACAGAGCTG GTAGAACTGC CAGGTACAAA GAAGATGGAG AAGCGTTATT AATTTTACTA 
CCTTCAGAAG AGCAAGGAAT GGTGCAGCAG CTTCTCCAGA AGAAAGTACC TGTGAAGGAG 
ATCAACTATT TGGCCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006510747.1
CDS	168..1508
Translation	MGKTVASLGQGTRPDPVRSFNRWKKKHSHRQHQKKERRKQLKKPEWQVEREGISRLMQNYEKINVNEITRFSDFPLSKKTLKGLQEAQYRLVTEIQKQTIGLALQGKDVLGAAKTGSGKTLAFLVPVLEALYRLQWTSTDGLGVLIISPTRELAYQTFEVLRKVGKNHDFSAGLIIGGKDLKHEAERINNINILVCTPGRLLQHMDETICFHATNLQMLVLDEADRILDMGFADTMNAIIENLPKKRQTLLFSATQTKSVKDLARLSLKDPEYVWVHEKAKYSTPATLEQNYIICELHQKISVLFSFLRSHLKKKSIVFFSSCKEVQYLYRVFCRLRPGISILALHGRQQQMRRMEVYNEFVRKRAAVLFATDIAARGLDFPAVNWVLQFDCPEDANTYIHRAGRTARYKEDGEALLILLPSEEQGMVQQLLQKKVPVKEINYLAI

Target ORF information:

RefSeq Version	XM_006510684.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 (Ddx10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006510684.4

ORF Insert Sequence:

ATGGGCAAAA CGGTTGCTTC TCTGGGTCAG GGAACCCGAC CCGACCCGGT GCGGAGCTTT 
AATCGCTGGA AGAAAAAACA CAGCCACAGG CAGCACCAAA AGAAGGAGCG GAGGAAGCAG 
CTAAAGAAGC CGGAATGGCA GGTCGAGCGC GAGGGGATCA GCCGCCTCAT GCAAAACTAC 
GAGAAGATAA ATGTAAATGA AATTACAAGA TTTTCAGATT TCCCTTTGTC CAAAAAAACA 
TTGAAAGGTT TGCAAGAAGC ACAGTACCGC TTGGTAACAG AGATACAGAA GCAGACCATA 
GGGTTGGCTT TGCAAGGTAA AGATGTGCTT GGTGCTGCCA AAACTGGATC TGGCAAGACT 
TTGGCTTTTC TTGTTCCGGT GTTGGAAGCC TTATACCGCC TGCAGTGGAC TTCAACAGAT 
GGCCTGGGAG TTCTGATAAT ATCACCTACT CGAGAACTGG CCTATCAGAC ATTTGAGGTT 
CTACGCAAAG TAGGGAAGAA TCATGATTTT TCAGCTGGTC TCATCATTGG TGGGAAGGAT 
CTGAAACATG AAGCTGAGAG GATCAACAAC ATAAACATAC TGGTTTGCAC ACCAGGTCGA 
CTTCTTCAAC ACATGGATGA AACAATATGT TTTCATGCCA CCAATCTTCA GATGTTAGTT 
CTTGATGAGG CAGATAGAAT CTTGGATATG GGCTTTGCTG ATACCATGAA TGCTATTATT 
GAAAATCTTC CCAAGAAACG CCAGACTTTA CTGTTCTCAG CCACACAGAC CAAATCTGTA 
AAGGACCTTG CGCGCTTGAG TTTGAAAGAC CCTGAGTATG TCTGGGTTCA TGAAAAAGCA 
AAATACAGCA CCCCTGCTAC GTTGGAACAG AACTATATAA TTTGTGAGCT GCACCAAAAA 
ATAAGTGTGC TGTTTTCCTT TTTGAGAAGC CACCTAAAGA AGAAGAGTAT TGTGTTTTTC 
TCCAGTTGCA AAGAGGTTCA GTATCTGTAT AGGGTGTTCT GCAGGCTGCG CCCAGGGATC 
TCGATCCTTG CTCTCCATGG CCGGCAGCAG CAGATGAGAA GAATGGAGGT CTACAATGAA 
TTTGTCCGAA AGAGGGCCGC AGTACTTTTT GCCACTGATA TTGCAGCTAG GGGACTGGAT 
TTTCCAGCTG TGAATTGGGT CCTTCAGTTT GATTGTCCAG AGGATGCCAA CACGTATATT 
CACAGAGCTG GTAGAACTGC CAGGTACAAA GAAGATGGAG AAGCGTTATT AATTTTACTA 
CCTTCAGAAG AGCAAGGAAT GGTGCAGCAG CTTCTCCAGA AGAAAGTACC TGTGAAGGAG 
ATCAACTATT TGGCCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx10 ( NM_029936.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084212.2 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 (Ddx10), mRNA.

Price & Availability↑

CloneID	OMu15395
Clone ID Related Accession (Same CDS sequence)	NM_029936.2
Accession Version	NM_029936.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent RNA helicase DDX10
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC160139.2 and AC112956.12. On or before Jun 8, 2008 this sequence version replaced XM_001481328.1, XM_284494.5, NM_029936.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK019495.1, BC080729.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGGCAAAA CGGTTGCTTC TCTGGGTCAG GGAACCCGAC CCGACCCGGT GCGGAGCTTT 
AATCGCTGGA AGAAAAAACA CAGCCACAGG CAGCACCAAA AGAAGGAGCG GAGGAAGCAG 
CTAAAGAAGC CGGAATGGCA GGTCGAGCGC GAGGGGATCA GCCGCCTCAT GCAAAACTAC 
GAGAAGATAA ATGTAAATGA AATTACAAGA TTTTCAGATT TCCCTTTGTC CAAAAAAACA 
TTGAAAGGTT TGCAAGAAGC ACAGTACCGC TTGGTAACAG AGATACAGAA GCAGACCATA 
GGGTTGGCTT TGCAAGGTAA AGATGTGCTT GGTGCTGCCA AAACTGGATC TGGCAAGACT 
TTGGCTTTTC TTGTTCCGGT GTTGGAAGCC TTATACCGCC TGCAGTGGAC TTCAACAGAT 
GGCCTGGGAG TTCTGATAAT ATCACCTACT CGAGAACTGG CCTATCAGAC ATTTGAGGTT 
CTACGCAAAG TAGGGAAGAA TCATGATTTT TCAGCTGGTC TCATCATTGG TGGGAAGGAT 
CTGAAACATG AAGCTGAGAG GATCAACAAC ATAAACATAC TGGTTTGCAC ACCAGGTCGA 
CTTCTTCAAC ACATGGATGA AACAATATGT TTTCATGCCA CCAATCTTCA GATGTTAGTT 
CTTGATGAGG CAGATAGAAT CTTGGATATG GGCTTTGCTG ATACCATGAA TGCTATTATT 
GAAAATCTTC CCAAGAAACG CCAGACTTTA CTGTTCTCAG CCACACAGAC CAAATCTGTA 
AAGGACCTTG CGCGCTTGAG TTTGAAAGAC CCTGAGTATG TCTGGGTTCA TGAAAAAGCA 
AAATACAGCA CCCCTGCTAC GTTGGAACAG AACTATATAA TTTGTGAGCT GCACCAAAAA 
ATAAGTGTGC TGTTTTCCTT TTTGAGAAGC CACCTAAAGA AGAAGAGTAT TGTGTTTTTC 
TCCAGTTGCA AAGAGGTTCA GTATCTGTAT AGGGTGTTCT GCAGGCTGCG CCCAGGGATC 
TCGATCCTTG CTCTCCATGG CCGGCAGCAG CAGATGAGAA GAATGGAGGT CTACAATGAA 
TTTGTCCGAA AGAGGGCCGC AGTACTTTTT GCCACTGATA TTGCAGCTAG GGGACTGGAT 
TTTCCAGCTG TGAATTGGGT CCTTCAGTTT GATTGTCCAG AGGATGCCAA CACGTATATT 
CACAGAGCTG GTAGAACTGC CAGGTACAAA GAAGATGGAG AAGCGTTATT AATTTTACTA 
CCTTCAGAAG AGCAAGGAAT GGTGCAGCAG CTTCTCCAGA AGAAAGTACC TGTGAAGGAG 
ATCAAAATCA ATCCAGAAAA ACTTATTGAT GTTCAGAAAA AATTAGAATC ATTTTTAGCT 
CAAGATCAAG ATTTAAAAGA AAGAGCTCAA AGGTGTTTTG TCTCCTATAT ACGCTCAGTA 
TATCTTATGA AGGATAAAGA AGTATTTAAT GTGAGCAAGT TACCTATTAC TGAATATGCA 
CTGTCTCTTG GTCTTGCTGT GGCACCGCGG ATAAGATTTC TTCAGAAACT GGAGAAGCAG 
CCCAGCACAG AATTGGTAAA GAATCCAGTC ACAGAAGCAG TTCCGCCCAG GGCCCCCTCC 
CTCCCCAACG ATGAAGCAGA CGAATCTCCA GCTTATGTCA GTGAGAAGAT GTCCGTCCTT 
CACAAAAGTG GGGAAAGACT GGAAGAGACA GAACACAGAC TGGCTAGTGG TGACGGTGAC 
GAAGAACAGG ATGAAGAGAC GGAAGACGAA GAGACGGAAG ATCATCTGGG AAAAGCAAGA 
GAGCCTCACA CTGAATCTGT TGTCAGTATT GAGGAGGCAC AGAAGGTCAA GGAAGTTTCT 
GTGCAGTTCT TAAACAGAGA TGATGATGAT GAAGATGGAC CTGATGCTGA CTTCCTCACA 
GTGAAGCGGC GGGATGTGTT TGGGTTGGAT CTTAAAGAGA ATGAAGCACT GTCGAAAAAA 
GAACCTTCTA AATCCAGTGT CAAGAAAAAA TTAACCAAAG TTGCAGAAGC AAAAAAAGTA 
ATGAAAAGAA GTTTTAAAGT GAACAAGAAA ATAACATTTA CTGATGAAGG GGAGTTGGTT 
CAGCAGTGGC CACAGATTCA GAAATGTGCT ATAAAAGATG TGGAGGAAGA AGATGACACT 
GGTGGTATCA ACCTAGACAA AGCAAAGGAA AGGCTTCAGG AAGAGGACAA ATTTGACAAA 
GAAGAATACA GGAAGAAAAT TAAGGCAAAG CACCGGGAAA GACGGCTAAA AGAAAGAGAG 
GCCAGAAGAG AAGCCAACAA AAGACAAGCA AAGGCCAGAG ATGAAGAGGA AGCCTTTCTG 
GACTGGAGTG ATGAAGATGA TGGTGGGTTT GATCCAAGTA CACTTCCAGA TCCTGACAAG 
CACAGAAGTT CTGAAGAGTC GGAGAGTGAA GATACAAACC ATAAGATGAG TGACACCAAG 
AAGAAGCAGG AAACAAGGAA GAGAAACAAC ACTGAAGACG ATGATGTAAG ACCAAGAAGT 
CGTCATGGAA AGAAGGCCAA GTGGGAAACG GTAGAACCTT TGGATACAGG CCTGTCTTTA 
GCAGAGGATG AAGAGCTGGT GTTACATCTC CTCAAAAGTC AAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084212.2
CDS	110..2737
Translation	MGKTVASLGQGTRPDPVRSFNRWKKKHSHRQHQKKERRKQLKKPEWQVEREGISRLMQNYEKINVNEITRFSDFPLSKKTLKGLQEAQYRLVTEIQKQTIGLALQGKDVLGAAKTGSGKTLAFLVPVLEALYRLQWTSTDGLGVLIISPTRELAYQTFEVLRKVGKNHDFSAGLIIGGKDLKHEAERINNINILVCTPGRLLQHMDETICFHATNLQMLVLDEADRILDMGFADTMNAIIENLPKKRQTLLFSATQTKSVKDLARLSLKDPEYVWVHEKAKYSTPATLEQNYIICELHQKISVLFSFLRSHLKKKSIVFFSSCKEVQYLYRVFCRLRPGISILALHGRQQQMRRMEVYNEFVRKRAAVLFATDIAARGLDFPAVNWVLQFDCPEDANTYIHRAGRTARYKEDGEALLILLPSEEQGMVQQLLQKKVPVKEIKINPEKLIDVQKKLESFLAQDQDLKERAQRCFVSYIRSVYLMKDKEVFNVSKLPITEYALSLGLAVAPRIRFLQKLEKQPSTELVKNPVTEAVPPRAPSLPNDEADESPAYVSEKMSVLHKSGERLEETEHRLASGDGDEEQDEETEDEETEDHLGKAREPHTESVVSIEEAQKVKEVSVQFLNRDDDDEDGPDADFLTVKRRDVFGLDLKENEALSKKEPSKSSVKKKLTKVAEAKKVMKRSFKVNKKITFTDEGELVQQWPQIQKCAIKDVEEEDDTGGINLDKAKERLQEEDKFDKEEYRKKIKAKHRERRLKEREARREANKRQAKARDEEEAFLDWSDEDDGGFDPSTLPDPDKHRSSEESESEDTNHKMSDTKKKQETRKRNNTEDDDVRPRSRHGKKAKWETVEPLDTGLSLAEDEELVLHLLKSQN

Target ORF information:

RefSeq Version	NM_029936.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 (Ddx10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029936.2

ORF Insert Sequence:

ATGGGCAAAA CGGTTGCTTC TCTGGGTCAG GGAACCCGAC CCGACCCGGT GCGGAGCTTT 
AATCGCTGGA AGAAAAAACA CAGCCACAGG CAGCACCAAA AGAAGGAGCG GAGGAAGCAG 
CTAAAGAAGC CGGAATGGCA GGTCGAGCGC GAGGGGATCA GCCGCCTCAT GCAAAACTAC 
GAGAAGATAA ATGTAAATGA AATTACAAGA TTTTCAGATT TCCCTTTGTC CAAAAAAACA 
TTGAAAGGTT TGCAAGAAGC ACAGTACCGC TTGGTAACAG AGATACAGAA GCAGACCATA 
GGGTTGGCTT TGCAAGGTAA AGATGTGCTT GGTGCTGCCA AAACTGGATC TGGCAAGACT 
TTGGCTTTTC TTGTTCCGGT GTTGGAAGCC TTATACCGCC TGCAGTGGAC TTCAACAGAT 
GGCCTGGGAG TTCTGATAAT ATCACCTACT CGAGAACTGG CCTATCAGAC ATTTGAGGTT 
CTACGCAAAG TAGGGAAGAA TCATGATTTT TCAGCTGGTC TCATCATTGG TGGGAAGGAT 
CTGAAACATG AAGCTGAGAG GATCAACAAC ATAAACATAC TGGTTTGCAC ACCAGGTCGA 
CTTCTTCAAC ACATGGATGA AACAATATGT TTTCATGCCA CCAATCTTCA GATGTTAGTT 
CTTGATGAGG CAGATAGAAT CTTGGATATG GGCTTTGCTG ATACCATGAA TGCTATTATT 
GAAAATCTTC CCAAGAAACG CCAGACTTTA CTGTTCTCAG CCACACAGAC CAAATCTGTA 
AAGGACCTTG CGCGCTTGAG TTTGAAAGAC CCTGAGTATG TCTGGGTTCA TGAAAAAGCA 
AAATACAGCA CCCCTGCTAC GTTGGAACAG AACTATATAA TTTGTGAGCT GCACCAAAAA 
ATAAGTGTGC TGTTTTCCTT TTTGAGAAGC CACCTAAAGA AGAAGAGTAT TGTGTTTTTC 
TCCAGTTGCA AAGAGGTTCA GTATCTGTAT AGGGTGTTCT GCAGGCTGCG CCCAGGGATC 
TCGATCCTTG CTCTCCATGG CCGGCAGCAG CAGATGAGAA GAATGGAGGT CTACAATGAA 
TTTGTCCGAA AGAGGGCCGC AGTACTTTTT GCCACTGATA TTGCAGCTAG GGGACTGGAT 
TTTCCAGCTG TGAATTGGGT CCTTCAGTTT GATTGTCCAG AGGATGCCAA CACGTATATT 
CACAGAGCTG GTAGAACTGC CAGGTACAAA GAAGATGGAG AAGCGTTATT AATTTTACTA 
CCTTCAGAAG AGCAAGGAAT GGTGCAGCAG CTTCTCCAGA AGAAAGTACC TGTGAAGGAG 
ATCAAAATCA ATCCAGAAAA ACTTATTGAT GTTCAGAAAA AATTAGAATC ATTTTTAGCT 
CAAGATCAAG ATTTAAAAGA AAGAGCTCAA AGGTGTTTTG TCTCCTATAT ACGCTCAGTA 
TATCTTATGA AGGATAAAGA AGTATTTAAT GTGAGCAAGT TACCTATTAC TGAATATGCA 
CTGTCTCTTG GTCTTGCTGT GGCACCGCGG ATAAGATTTC TTCAGAAACT GGAGAAGCAG 
CCCAGCACAG AATTGGTAAA GAATCCAGTC ACAGAAGCAG TTCCGCCCAG GGCCCCCTCC 
CTCCCCAACG ATGAAGCAGA CGAATCTCCA GCTTATGTCA GTGAGAAGAT GTCCGTCCTT 
CACAAAAGTG GGGAAAGACT GGAAGAGACA GAACACAGAC TGGCTAGTGG TGACGGTGAC 
GAAGAACAGG ATGAAGAGAC GGAAGACGAA GAGACGGAAG ATCATCTGGG AAAAGCAAGA 
GAGCCTCACA CTGAATCTGT TGTCAGTATT GAGGAGGCAC AGAAGGTCAA GGAAGTTTCT 
GTGCAGTTCT TAAACAGAGA TGATGATGAT GAAGATGGAC CTGATGCTGA CTTCCTCACA 
GTGAAGCGGC GGGATGTGTT TGGGTTGGAT CTTAAAGAGA ATGAAGCACT GTCGAAAAAA 
GAACCTTCTA AATCCAGTGT CAAGAAAAAA TTAACCAAAG TTGCAGAAGC AAAAAAAGTA 
ATGAAAAGAA GTTTTAAAGT GAACAAGAAA ATAACATTTA CTGATGAAGG GGAGTTGGTT 
CAGCAGTGGC CACAGATTCA GAAATGTGCT ATAAAAGATG TGGAGGAAGA AGATGACACT 
GGTGGTATCA ACCTAGACAA AGCAAAGGAA AGGCTTCAGG AAGAGGACAA ATTTGACAAA 
GAAGAATACA GGAAGAAAAT TAAGGCAAAG CACCGGGAAA GACGGCTAAA AGAAAGAGAG 
GCCAGAAGAG AAGCCAACAA AAGACAAGCA AAGGCCAGAG ATGAAGAGGA AGCCTTTCTG 
GACTGGAGTG ATGAAGATGA TGGTGGGTTT GATCCAAGTA CACTTCCAGA TCCTGACAAG 
CACAGAAGTT CTGAAGAGTC GGAGAGTGAA GATACAAACC ATAAGATGAG TGACACCAAG 
AAGAAGCAGG AAACAAGGAA GAGAAACAAC ACTGAAGACG ATGATGTAAG ACCAAGAAGT 
CGTCATGGAA AGAAGGCCAA GTGGGAAACG GTAGAACCTT TGGATACAGG CCTGTCTTTA 
GCAGAGGATG AAGAGCTGGT GTTACATCTC CTCAAAAGTC AAAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Discovery and characterization of spontaneous mouse models of craniofacial dysmorphology.
Developmental biology415(2)216-227(2016 07)
Palmer K,Fairfield H,Borgeia S,Curtain M,Hassan MG,Dionne L,Yong Karst S,Coombs H,Bronson RT,Reinholdt LG,Bergstrom DE,Donahue LR,Cox TC,Murray SA

Exome sequencing reveals pathogenic mutations in 91 strains of mice with Mendelian disorders.
Genome research25(7)948-57(2015 Jul)
Fairfield H,Srivastava A,Ananda G,Liu R,Kircher M,Lakshminarayana A,Harris BS,Karst SY,Dionne LA,Kane CC,Curtain M,Berry ML,Ward-Bailey PF,Greenstein I,Byers C,Czechanski A,Sharp J,Palmer K,Gudis P,Martin W,Tadenev A,Bogdanik L,Pratt CH,Chang B,Schroeder DG,Cox GA,Cliften P,Milbrandt J,Murray S,Burgess R,Bergstrom DE,Donahue LR,Hamamy H,Masri A,Santoni FA,Makrythanasis P,Antonarakis SE,Shendure J,Reinholdt LG

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Ddx10 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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