ddx10 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddx10
Entrez Gene ID	613047
Full Name	DEAD-box helicase 10
General protein information	Preferred Names DEAD-box helicase 10 Names probable ATP-dependent RNA helicase DDX10 DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018091309.1	XP_017946798.1	probable ATP-dependent RNA helicase DDX10 isoform X1
NM_001102681.1	NP_001096151.1	probable ATP-dependent RNA helicase DDX10

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DDX10	XP_001141618.1
Canis lupus familiaris (dog)	DDX10	XP_536583.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001057	XP_001230842.3
Mus musculus (house mouse)	Ddx10	NP_084212.2
Drosophila melanogaster (fruit fly)	CG5800	NP_573230.1
Caenorhabditis elegans (roundworm)	Y23H5B.6	NP_490989.1
Danio rerio (zebrafish)	ddx10	XP_001922220.1
Homo sapiens (human)	DDX10	NP_004389.2
Rattus norvegicus (Norway rat)	Ddx10	NP_001100290.1
Xenopus tropicalis (tropical clawed frog)	ddx10	NP_001096151.1
Bos taurus (cattle)	DDX10	NP_001070349.1
Gallus gallus (chicken)	DDX10	NP_001073193.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ddx10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddx10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa02936	NM_001102681.1 Latest version!	Xenopus tropicalis DEAD-box helicase 10 (ddx10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa42583	XM_018091309.1 Latest version!	Xenopus tropicalis DEAD-box helicase 10 (ddx10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddx10 ( NM_001102681.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001096151.1 Xenopus tropicalis DEAD-box helicase 10 (ddx10), mRNA.

Price & Availability↑

CloneID	OXa02936
Clone ID Related Accession (Same CDS sequence)	NM_001102681.1
Accession Version	NM_001102681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX10
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC146615.1. ##Evidence-Data-START## Transcript exon combination :: BC146615.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGGGGAGC CGAGCCAGAA AGAGACCAAG AAGCCGCTGA TCAACAGCAG GAATGATCCG 
GTGAAAAACT TCGAGAAGTG GAAGAAGAAA TACAGTAAGA AGAAGGAAAA GTGCAAGAGC 
CACAGGAAGC AGAATAAGAA ACCCGAGTGG CAGGTGGAAA AGGAGACCAT TACTCGCCTG 
GAACAGCGCT ACTCGGAGAT CAATACCAGT GAGATTGTAA AATTCTCAGA TTTCCCTCTG 
TCCAAAAAGA CACTGAGAGG TTTGGTGGAA GGCCAATACC GATTACCTAC AGAGATCCAG 
AAACAGACAA TAGGCTTGGC CTTACAGGGA AAGGATGTGC TTGGCGCTGC CAAAACTGGC 
TCCGGGAAAA CACTGGCCTT CATTATTCCT GCTTTGGAAT GTTTATACCG CCAGCAGTGG 
ACATCCGATG ACGGACTTGG GGTCCTCATC ATATCACCCA CTCGAGAGCT TGCATATCAA 
ACATTTGAGG TATTGAGGAA AGTCGGGCGC AACCATGAAT TCTCAGCGGG GCTTGTCATT 
GGTGGAAAGG ATTTGAAGCA AGAGACAGCG TGCATCCACA GGACAAATAT ACTGATTTGT 
ACTCCCGGCC GGCTTTTGCA ACACATGGAT GAAACGTCTA CCTTTCATGC ATCTAATCTT 
CAGATGCTGG TTCTTGATGA AGCAGACAGA ATACTGGACA TGGGGTTTGC AGACACAATG 
AATGCCATTG TAGAGAACCT CCCTAAGAAA CGACAGACAC TCCTCTTCTC AGCGACGCAG 
ACAAAATCTG TCAAGGATCT TGCGCGCCTC AGCTTAAAGG ACCCTGAATA TGTGTGGGTG 
CATGAAAATG CCAAGTTCAG CACCCCCGCC ACTCTGGAAC AGAACTATGT TGTCTGCGAA 
CTACAGCAAA AAATCAACTT GCTCTACTCC TTTATAAGGA ATCACCTGAA GAAGAAGAGC 
ATTGTTTTCT TCTCGAGCTG CAAGGAGGTG CAGTACTTGT TCCGTGCATT CTGCAGGTTG 
CGGCCTGGTA TTCCCGTACT CGTTCTGCAT GGGAAGCAGC AGCAAATGAA GAGAATGGAA 
GTTTACAATG ACTTTATACG AAAGAAATCT GCAGTGCTGT TTGCTACAGA CATCGCAGCC 
AGAGGGTTAG ATTTTCCAGC CGTGAACTGG GTGCTTCAGC TCGACTGCCC TGAAGATGCC 
AATACGTACA TTCACAGAGT TGGAAGAACA GCCCGATACA AGGAAGGAGG AGAAGCCCTG 
CTTGTTCTGC TTCCCTCCGA AGTGAAAGGA ATGTTCAAAC AGCTAGAGGA GAAGAAAGTT 
CCTATTAATG AAATTAAAAT TAACCCTGAA AAGCTCATGG ATGTGCAAGG CCGTTTGGAA 
GCTTTTTTGG CGCAGGAGCA AGACCTAAAA GAGACGGCAC AAAGGTGCTT CGTCTCCTAC 
TTGCGCTCCG TTTATTTGAT GAAGAACAAG GAAGTTTTTG ATGTTTTCAA GTTGCCCCTG 
ACTCAGTATG CCCAGTCCTT AGGCCTTGCA GTTGCTCCAC GGGTTAGGTT CCTTCTCAAA 
GCTCAAAATG CTCAGTCAAC GTCTGTGAGC AAAGATGACC CCGGCACAGC AAGTGATCAA 
GAAATAGAGG CATTCAAGTC AAAGCTTGGT GGTAAACCAG CTGATGACCA AAGCAGAGAT 
GATGCTGACA ATGTCAATTC TTCAGAGGAA GAGAGTAATC TAGAAGAAGA CTCTGAGGAT 
GACGAGCAAG AGCTTTCAGA CAACCTAAAG CAAACAAGTT TGCAGTTTAT GAATGAGGAT 
GAAGACGATG ATGACTGTCG AGATCTTGAT TTCCTCACAG TGAAACGGAG AGATGTTTTT 
GGCATAGAGG ATGGCAGCTT TATTCCCGAA ACTGAAATGA CCAAATCCAA GTTGAAGAAA 
ACAGAGAAAG TATCTAAAGC AAAGGAGGCG AAGAAGGTTC TCAAGAAGAA ATTTCAAGTT 
AACACCAAAA TTGTCTTTGC GGATGACGGA GAGGTGATCC AGCAGTGGCC CCAAATCCAA 
AAAACTACAG TGAAAGAGAC AGAAGAGGAG GATGATTCAA GTGGCATCAA TCTGGCAAAG 
GCAAAGGAGC GGCTGAACGA GGAGGATAGG TTTGACAAAG AAGCTTACAG GATGAAAATC 
AAGGAGAAGC ACAGGGAGAA GAGGCTGAAA GAGAAGGCAG CCAGAAGAGC AGCCAACAAG 
AAAGAATCAG AGGAAGAGGA TGAAGCCGTG GCTTATCTTG ACCATGATGG CAGTGAAGAT 
GAACTTGACA TCAGTGCTCT TCCTGATCCC GACAAATACA GAGACATGGA GGAAAGTGAT 
GGTGGATCAG CACAGGAAAG CGCAAGTGAT CATGAAGAAG AGCGGCCGAA GAAGAGAACA 
TACCAAAGCC TTGCCGGAAA CCAAGCTGAA AAGTCTGTTG GCATCAAAAA GAAGCGGGCG 
AAACAGAACT CCGATGAGGA CTTTGAACCT CTTAACACTG GACTTTCTCT GGCAGAGGAC 
GAGGAGCTTG TGTTGCACCT ACTGGGCCAC AGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096151.1
CDS	29..2587
Translation	MGEPSQKETKKPLINSRNDPVKNFEKWKKKYSKKKEKCKSHRKQNKKPEWQVEKETITRLEQRYSEINTSEIVKFSDFPLSKKTLRGLVEGQYRLPTEIQKQTIGLALQGKDVLGAAKTGSGKTLAFIIPALECLYRQQWTSDDGLGVLIISPTRELAYQTFEVLRKVGRNHEFSAGLVIGGKDLKQETACIHRTNILICTPGRLLQHMDETSTFHASNLQMLVLDEADRILDMGFADTMNAIVENLPKKRQTLLFSATQTKSVKDLARLSLKDPEYVWVHENAKFSTPATLEQNYVVCELQQKINLLYSFIRNHLKKKSIVFFSSCKEVQYLFRAFCRLRPGIPVLVLHGKQQQMKRMEVYNDFIRKKSAVLFATDIAARGLDFPAVNWVLQLDCPEDANTYIHRVGRTARYKEGGEALLVLLPSEVKGMFKQLEEKKVPINEIKINPEKLMDVQGRLEAFLAQEQDLKETAQRCFVSYLRSVYLMKNKEVFDVFKLPLTQYAQSLGLAVAPRVRFLLKAQNAQSTSVSKDDPGTASDQEIEAFKSKLGGKPADDQSRDDADNVNSSEEESNLEEDSEDDEQELSDNLKQTSLQFMNEDEDDDDCRDLDFLTVKRRDVFGIEDGSFIPETEMTKSKLKKTEKVSKAKEAKKVLKKKFQVNTKIVFADDGEVIQQWPQIQKTTVKETEEEDDSSGINLAKAKERLNEEDRFDKEAYRMKIKEKHREKRLKEKAARRAANKKESEEEDEAVAYLDHDGSEDELDISALPDPDKYRDMEESDGGSAQESASDHEEERPKKRTYQSLAGNQAEKSVGIKKKRAKQNSDEDFEPLNTGLSLAEDEELVLHLLGHSS

Target ORF information:

RefSeq Version	NM_001102681.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 10 (ddx10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102681.1

ORF Insert Sequence:

ATGGGGGAGC CGAGCCAGAA AGAGACCAAG AAGCCGCTGA TCAACAGCAG GAATGATCCG 
GTGAAAAACT TCGAGAAGTG GAAGAAGAAA TACAGTAAGA AGAAGGAAAA GTGCAAGAGC 
CACAGGAAGC AGAATAAGAA ACCCGAGTGG CAGGTGGAAA AGGAGACCAT TACTCGCCTG 
GAACAGCGCT ACTCGGAGAT CAATACCAGT GAGATTGTAA AATTCTCAGA TTTCCCTCTG 
TCCAAAAAGA CACTGAGAGG TTTGGTGGAA GGCCAATACC GATTACCTAC AGAGATCCAG 
AAACAGACAA TAGGCTTGGC CTTACAGGGA AAGGATGTGC TTGGCGCTGC CAAAACTGGC 
TCCGGGAAAA CACTGGCCTT CATTATTCCT GCTTTGGAAT GTTTATACCG CCAGCAGTGG 
ACATCCGATG ACGGACTTGG GGTCCTCATC ATATCACCCA CTCGAGAGCT TGCATATCAA 
ACATTTGAGG TATTGAGGAA AGTCGGGCGC AACCATGAAT TCTCAGCGGG GCTTGTCATT 
GGTGGAAAGG ATTTGAAGCA AGAGACAGCG TGCATCCACA GGACAAATAT ACTGATTTGT 
ACTCCCGGCC GGCTTTTGCA ACACATGGAT GAAACGTCTA CCTTTCATGC ATCTAATCTT 
CAGATGCTGG TTCTTGATGA AGCAGACAGA ATACTGGACA TGGGGTTTGC AGACACAATG 
AATGCCATTG TAGAGAACCT CCCTAAGAAA CGACAGACAC TCCTCTTCTC AGCGACGCAG 
ACAAAATCTG TCAAGGATCT TGCGCGCCTC AGCTTAAAGG ACCCTGAATA TGTGTGGGTG 
CATGAAAATG CCAAGTTCAG CACCCCCGCC ACTCTGGAAC AGAACTATGT TGTCTGCGAA 
CTACAGCAAA AAATCAACTT GCTCTACTCC TTTATAAGGA ATCACCTGAA GAAGAAGAGC 
ATTGTTTTCT TCTCGAGCTG CAAGGAGGTG CAGTACTTGT TCCGTGCATT CTGCAGGTTG 
CGGCCTGGTA TTCCCGTACT CGTTCTGCAT GGGAAGCAGC AGCAAATGAA GAGAATGGAA 
GTTTACAATG ACTTTATACG AAAGAAATCT GCAGTGCTGT TTGCTACAGA CATCGCAGCC 
AGAGGGTTAG ATTTTCCAGC CGTGAACTGG GTGCTTCAGC TCGACTGCCC TGAAGATGCC 
AATACGTACA TTCACAGAGT TGGAAGAACA GCCCGATACA AGGAAGGAGG AGAAGCCCTG 
CTTGTTCTGC TTCCCTCCGA AGTGAAAGGA ATGTTCAAAC AGCTAGAGGA GAAGAAAGTT 
CCTATTAATG AAATTAAAAT TAACCCTGAA AAGCTCATGG ATGTGCAAGG CCGTTTGGAA 
GCTTTTTTGG CGCAGGAGCA AGACCTAAAA GAGACGGCAC AAAGGTGCTT CGTCTCCTAC 
TTGCGCTCCG TTTATTTGAT GAAGAACAAG GAAGTTTTTG ATGTTTTCAA GTTGCCCCTG 
ACTCAGTATG CCCAGTCCTT AGGCCTTGCA GTTGCTCCAC GGGTTAGGTT CCTTCTCAAA 
GCTCAAAATG CTCAGTCAAC GTCTGTGAGC AAAGATGACC CCGGCACAGC AAGTGATCAA 
GAAATAGAGG CATTCAAGTC AAAGCTTGGT GGTAAACCAG CTGATGACCA AAGCAGAGAT 
GATGCTGACA ATGTCAATTC TTCAGAGGAA GAGAGTAATC TAGAAGAAGA CTCTGAGGAT 
GACGAGCAAG AGCTTTCAGA CAACCTAAAG CAAACAAGTT TGCAGTTTAT GAATGAGGAT 
GAAGACGATG ATGACTGTCG AGATCTTGAT TTCCTCACAG TGAAACGGAG AGATGTTTTT 
GGCATAGAGG ATGGCAGCTT TATTCCCGAA ACTGAAATGA CCAAATCCAA GTTGAAGAAA 
ACAGAGAAAG TATCTAAAGC AAAGGAGGCG AAGAAGGTTC TCAAGAAGAA ATTTCAAGTT 
AACACCAAAA TTGTCTTTGC GGATGACGGA GAGGTGATCC AGCAGTGGCC CCAAATCCAA 
AAAACTACAG TGAAAGAGAC AGAAGAGGAG GATGATTCAA GTGGCATCAA TCTGGCAAAG 
GCAAAGGAGC GGCTGAACGA GGAGGATAGG TTTGACAAAG AAGCTTACAG GATGAAAATC 
AAGGAGAAGC ACAGGGAGAA GAGGCTGAAA GAGAAGGCAG CCAGAAGAGC AGCCAACAAG 
AAAGAATCAG AGGAAGAGGA TGAAGCCGTG GCTTATCTTG ACCATGATGG CAGTGAAGAT 
GAACTTGACA TCAGTGCTCT TCCTGATCCC GACAAATACA GAGACATGGA GGAAAGTGAT 
GGTGGATCAG CACAGGAAAG CGCAAGTGAT CATGAAGAAG AGCGGCCGAA GAAGAGAACA 
TACCAAAGCC TTGCCGGAAA CCAAGCTGAA AAGTCTGTTG GCATCAAAAA GAAGCGGGCG 
AAACAGAACT CCGATGAGGA CTTTGAACCT CTTAACACTG GACTTTCTCT GGCAGAGGAC 
GAGGAGCTTG TGTTGCACCT ACTGGGCCAC AGCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx10 ( XM_018091309.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946798.1 Xenopus tropicalis DEAD-box helicase 10 (ddx10), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa42583
Clone ID Related Accession (Same CDS sequence)	XM_018091309.1
Accession Version	XM_018091309.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGC CGAGCCAGAA AGAGACCAAG AAGCCGCTGA TCAACAGCAG GAATGATCCG 
GTGAAAAACT TCGAGAAGTG GAAGAAGAAA TACAGTAAGA AGAAGGAAAA GTGCAAGAGC 
CACAGGAAGC AGAATAAGAA ACCCGAGTGG CAGGTGGAAA AGGAGACCAT TACTCGCCTG 
GAACAGCGCT ACTCGGAGAT CAATACCAGT GAGATTGTAA AATTCTCAGA TTTCCCTCTG 
TCCAAAAAGA CACTGAGAGG TTTGGTGGAA GGCCAATACC GATTACCTAC AGAGATCCAG 
AAACAGACAA TAGGCTTGGC CTTACAGGGA AAGGATGTGC TTGGCGCTGC CAAAACTGGC 
TCCGGGAAAA CACTGGCCTT CATTATTCCT GCTTTGGAAT GTTTATACCG CCAGCAGTGG 
ACATCCGATG ACGGACTTGG GGTCCTCATC ATATCACCCA CTCGAGAGCT TGCATATCAA 
ACATTTGAGG TATTGAGGAA AGTCGGGCGC AACCATGAAT TCTCAGCGGG GCTTGTCATT 
GGTGGAAAGG ATTTGAAGCA AGAGACAGCG TGCATCCACA GGACAAATAT ACTGATTTGT 
ACTCCCGGCC GGCTTTTGCA ACACATGGAT GAAACGTCTA CCTTTCATGC ATCTAATCTT 
CAGATGCTGG TTCTTGATGA AGCAGACAGA ATACTGGACA TGGGGTTTGC AGACACAATG 
AATGCCATTG TAGAGAACCT CCCTAAGAAA CGACAGACAC TCCTCTTCTC AGCGACGCAG 
ACAAAATCTG TCAAGGATCT TGCGCGCCTC AGCTTAAAGG ACCCTGAATA TGTGTGGGTG 
CATGAAAATG CCAAGTTCAG CACCCCCGCC ACTCTGGAAC AGAACTATGT TGTCTGCGAA 
CTACAGCAAA AAATCAACTT GCTCTACTCC TTTATAAGGA ATCACCTGAA GAAGAAGAGC 
ATTGTTTTCT TCTCGAGCTG CAAGGAGGTG CAGTACTTGT TCCGTGCATT CTGCAGGTTG 
CGGCCTGGTA TTCCCGTACT CGTTCTGCAT GGGAAGCAGC AGCAAATGAA GAGAATGGAA 
GTTTACAATG ACTTTATACG AAAGAAATCT GCAGTGCTGT TTGCTACAGA CATCGCAGCC 
AGAGGGTTAG ATTTTCCAGC CGTGAACTGG GTGCTTCAGC TCGACTGCCC TGAAGATGCC 
AATACGTACA TTCACAGAGT TGGAAGAACA GCCCGATACA AGGAAGGAGG AGAAGCCCTG 
CTTGTTCTGC TTCCCTCCGA AGTGAAAGGA ATGTTCAAAC AGCTAGAGGA GAAGAAAGTT 
CCTATTAATG AAATTAAAAT TAACCCTGAA AAGCTCATGG ATGTGCAAGG CCGTTTGGAA 
GCTTTTTTGG CGCAGGAGCA AGACCTAAAA GAGACGGCAC AAAGGTGCTT CGTCTCCTAC 
TTGCGCTCCG TTTATTTGAT GAAGAACAAG GAAGTTTTTG ATGTTTTCAA GTTGCCCCTG 
ACTCAGTATG CCCAGTCCTT AGGCCTTGCA GTTGCTCCAC GGGTTAGGTT CCTTCTCAAA 
GCTCAAAATG CTCAGTCAAC GTCTGTGAGC AAAGATGACC CCGGCACAGC AAGTGATCAA 
GAAATAGAGG CATTCAAGTC AAAGCTTGGT GGTAAACCAG CTGATGACCA AAGCAGAGAT 
GATGCTGACA ATGTCAATTC TTCAGAGGAA GAGAGTAATC TAGAAGAAGA CTCTGAGGAT 
GACGAGCAAG AGCTTTCAGA CAACCTAAAG CAAACAAGTT TGCAGTTTAT GAATGAGGAT 
GAAGACGATG ATGACTGTCG AGATCTTGAT TTCCTCACAG TGAAACGGAG AGATGTTTTT 
GGCATAGAGG ATGGCAGCTT TATTCCCGAA ACTGAAATGA CCAAATCCAA GTTGAAGAAA 
ACAGAGAAAG TATCTAAAGC AAAGGAGGCG AAGAAGGTTC TCAAGAAGAA ATTTCAAGTT 
AACACCAAAA TTGTCTTTGC GGATGACGGA GAGGTGATCC AGCAGTGGCC CCAAATCCAA 
AAAACTACAG TGAAAGAGAC AGAAGAGGAG GATGATTCAA GTGGCATCAA TCTGGCAAAG 
GCAAAGGAGC GGCTGAACGA GGAGGATAGG TTTGACAAAG AAGCTTACAG GATGAAAATC 
AAGGAGAAGC ACAGGGAGAA GAGGCTGAAA GAGAAGGCAG CCAGAAGAGC AGCCAACAAG 
AAAGAATCAG AGTGTTTGAC AAGATATTTT ACAGATGTGG TCACTATATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946798.1
CDS	89..2359
Translation	MGEPSQKETKKPLINSRNDPVKNFEKWKKKYSKKKEKCKSHRKQNKKPEWQVEKETITRLEQRYSEINTSEIVKFSDFPLSKKTLRGLVEGQYRLPTEIQKQTIGLALQGKDVLGAAKTGSGKTLAFIIPALECLYRQQWTSDDGLGVLIISPTRELAYQTFEVLRKVGRNHEFSAGLVIGGKDLKQETACIHRTNILICTPGRLLQHMDETSTFHASNLQMLVLDEADRILDMGFADTMNAIVENLPKKRQTLLFSATQTKSVKDLARLSLKDPEYVWVHENAKFSTPATLEQNYVVCELQQKINLLYSFIRNHLKKKSIVFFSSCKEVQYLFRAFCRLRPGIPVLVLHGKQQQMKRMEVYNDFIRKKSAVLFATDIAARGLDFPAVNWVLQLDCPEDANTYIHRVGRTARYKEGGEALLVLLPSEVKGMFKQLEEKKVPINEIKINPEKLMDVQGRLEAFLAQEQDLKETAQRCFVSYLRSVYLMKNKEVFDVFKLPLTQYAQSLGLAVAPRVRFLLKAQNAQSTSVSKDDPGTASDQEIEAFKSKLGGKPADDQSRDDADNVNSSEEESNLEEDSEDDEQELSDNLKQTSLQFMNEDEDDDDCRDLDFLTVKRRDVFGIEDGSFIPETEMTKSKLKKTEKVSKAKEAKKVLKKKFQVNTKIVFADDGEVIQQWPQIQKTTVKETEEEDDSSGINLAKAKERLNEEDRFDKEAYRMKIKEKHREKRLKEKAARRAANKKESECLTRYFTDVVTI

Target ORF information:

RefSeq Version	XM_018091309.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 10 (ddx10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091309.1

ORF Insert Sequence:

ATGGGGGAGC CGAGCCAGAA AGAGACCAAG AAGCCGCTGA TCAACAGCAG GAATGATCCG 
GTGAAAAACT TCGAGAAGTG GAAGAAGAAA TACAGTAAGA AGAAGGAAAA GTGCAAGAGC 
CACAGGAAGC AGAATAAGAA ACCCGAGTGG CAGGTGGAAA AGGAGACCAT TACTCGCCTG 
GAACAGCGCT ACTCGGAGAT CAATACCAGT GAGATTGTAA AATTCTCAGA TTTCCCTCTG 
TCCAAAAAGA CACTGAGAGG TTTGGTGGAA GGCCAATACC GATTACCTAC AGAGATCCAG 
AAACAGACAA TAGGCTTGGC CTTACAGGGA AAGGATGTGC TTGGCGCTGC CAAAACTGGC 
TCCGGGAAAA CACTGGCCTT CATTATTCCT GCTTTGGAAT GTTTATACCG CCAGCAGTGG 
ACATCCGATG ACGGACTTGG GGTCCTCATC ATATCACCCA CTCGAGAGCT TGCATATCAA 
ACATTTGAGG TATTGAGGAA AGTCGGGCGC AACCATGAAT TCTCAGCGGG GCTTGTCATT 
GGTGGAAAGG ATTTGAAGCA AGAGACAGCG TGCATCCACA GGACAAATAT ACTGATTTGT 
ACTCCCGGCC GGCTTTTGCA ACACATGGAT GAAACGTCTA CCTTTCATGC ATCTAATCTT 
CAGATGCTGG TTCTTGATGA AGCAGACAGA ATACTGGACA TGGGGTTTGC AGACACAATG 
AATGCCATTG TAGAGAACCT CCCTAAGAAA CGACAGACAC TCCTCTTCTC AGCGACGCAG 
ACAAAATCTG TCAAGGATCT TGCGCGCCTC AGCTTAAAGG ACCCTGAATA TGTGTGGGTG 
CATGAAAATG CCAAGTTCAG CACCCCCGCC ACTCTGGAAC AGAACTATGT TGTCTGCGAA 
CTACAGCAAA AAATCAACTT GCTCTACTCC TTTATAAGGA ATCACCTGAA GAAGAAGAGC 
ATTGTTTTCT TCTCGAGCTG CAAGGAGGTG CAGTACTTGT TCCGTGCATT CTGCAGGTTG 
CGGCCTGGTA TTCCCGTACT CGTTCTGCAT GGGAAGCAGC AGCAAATGAA GAGAATGGAA 
GTTTACAATG ACTTTATACG AAAGAAATCT GCAGTGCTGT TTGCTACAGA CATCGCAGCC 
AGAGGGTTAG ATTTTCCAGC CGTGAACTGG GTGCTTCAGC TCGACTGCCC TGAAGATGCC 
AATACGTACA TTCACAGAGT TGGAAGAACA GCCCGATACA AGGAAGGAGG AGAAGCCCTG 
CTTGTTCTGC TTCCCTCCGA AGTGAAAGGA ATGTTCAAAC AGCTAGAGGA GAAGAAAGTT 
CCTATTAATG AAATTAAAAT TAACCCTGAA AAGCTCATGG ATGTGCAAGG CCGTTTGGAA 
GCTTTTTTGG CGCAGGAGCA AGACCTAAAA GAGACGGCAC AAAGGTGCTT CGTCTCCTAC 
TTGCGCTCCG TTTATTTGAT GAAGAACAAG GAAGTTTTTG ATGTTTTCAA GTTGCCCCTG 
ACTCAGTATG CCCAGTCCTT AGGCCTTGCA GTTGCTCCAC GGGTTAGGTT CCTTCTCAAA 
GCTCAAAATG CTCAGTCAAC GTCTGTGAGC AAAGATGACC CCGGCACAGC AAGTGATCAA 
GAAATAGAGG CATTCAAGTC AAAGCTTGGT GGTAAACCAG CTGATGACCA AAGCAGAGAT 
GATGCTGACA ATGTCAATTC TTCAGAGGAA GAGAGTAATC TAGAAGAAGA CTCTGAGGAT 
GACGAGCAAG AGCTTTCAGA CAACCTAAAG CAAACAAGTT TGCAGTTTAT GAATGAGGAT 
GAAGACGATG ATGACTGTCG AGATCTTGAT TTCCTCACAG TGAAACGGAG AGATGTTTTT 
GGCATAGAGG ATGGCAGCTT TATTCCCGAA ACTGAAATGA CCAAATCCAA GTTGAAGAAA 
ACAGAGAAAG TATCTAAAGC AAAGGAGGCG AAGAAGGTTC TCAAGAAGAA ATTTCAAGTT 
AACACCAAAA TTGTCTTTGC GGATGACGGA GAGGTGATCC AGCAGTGGCC CCAAATCCAA 
AAAACTACAG TGAAAGAGAC AGAAGAGGAG GATGATTCAA GTGGCATCAA TCTGGCAAAG 
GCAAAGGAGC GGCTGAACGA GGAGGATAGG TTTGACAAAG AAGCTTACAG GATGAAAATC 
AAGGAGAAGC ACAGGGAGAA GAGGCTGAAA GAGAAGGCAG CCAGAAGAGC AGCCAACAAG 
AAAGAATCAG AGTGTTTGAC AAGATATTTT ACAGATGTGG TCACTATATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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ddx10 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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