Kras cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kras
Entrez Gene ID	24525
Full Name	KRAS proto-oncogene, GTPase
Synonyms	Kras2,c-Ki-ras,p21
General protein information	Preferred Names KRAS proto-oncogene, GTPase Names GTPase KRas K-Ras 2 Kirsten rat sarcoma viral oncogene homolog Kirsten rat sarcoma viral oncogene homologue 2 (active) c-K-ras c-K-ras2 protein c-Kirsten-ras proto-oncogene ki-Ras v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q44
Summary	oncogene and member of the small GTPase superfamily [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017592443.1	XP_017447932.1	GTPase KRas isoform X1
XM_017592442.1	XP_017447931.1	GTPase KRas isoform X1
XM_008763354.2	XP_008761576.1	GTPase KRas isoform X2
XM_008763355.2	XP_008761577.1	GTPase KRas isoform X3
NM_031515.3	NP_113703.1	GTPase KRas

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	KRAS	XP_003433608.2
Homo sapiens (human)	KRAS	NP_203524.1
Drosophila melanogaster (fruit fly)	Ras85D	NP_476699.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002219	XP_307965.4
Xenopus tropicalis (tropical clawed frog)	LOC100485959	XP_004920180.1
Pan troglodytes (chimpanzee)	KRAS	XP_003313842.1
Mus musculus (house mouse)	Kras	NP_067259.4
Caenorhabditis elegans (roundworm)	let-60	NP_502213.3
Bos taurus (cattle)	KRAS	NP_001103471.1
Rattus norvegicus (Norway rat)	Kras	NP_113703.1
Gallus gallus (chicken)	KRAS	NP_001243091.1
Danio rerio (zebrafish)	kras	NP_001003744.1

Gene Ontology
Bibliography

Related articles in PubMed

Wild type Kirsten rat sarcoma is a novel microRNA-622-regulated therapeutic target for hepatocellular carcinoma and contributes to sorafenib resistance.
Dietrich P, Koch A, Fritz V, Hartmann A, Bosserhoff AK, Hellerbrand C
Gut(2017 Dec)

The ALPPS Approach for Colorectal Liver Metastases: Impact of KRAS Mutation Status in Survival.
Serenari M, Alvarez FA, Ardiles V, de Santiba?es M, Pekolj J, de Santiba?es E
Digestive surgery(2017 Oct)

Mucinous cystic neoplasms of the liver and pancreas: relationship between KRAS driver mutations and disease progression.
Fujikura K, Akita M, Abe-Suzuki S, Itoh T, Zen Y
Histopathology71(4)591-600(2017 Oct)

Activating KRAS mutation is prognostic only among patients who receive preoperative chemotherapy before resection of colorectal liver metastases.
Margonis GA, Kim Y, Sasaki K, Samaha M, Buettner S, Amini N, Pawlik TM
Journal of surgical oncology114(3)361-7(2016 Sep)

Nucleotide sequence of exon I of the rat c-K-ras gene.
Iritani A, Katayama N, Tahira T, Hayashi K, Tsuchida N
The Bulletin of Tokyo Medical and Dental University33(1)35-40(1986 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Kras protein mutation is responsible for the development of mucinous adenocarcinoma of the lung in patient born with congenital pulmonary malformation.
Title: Pulmonary Kirsten Rat Sarcoma Virus Mutation Positive Mucinous Adenocarcinoma Arising in a?Congenital Pulmonary Airway?Malformation, Mixed Type 1 and 2.

Taken together, these results reveal the key role of histone H3Kme in TGF-beta1 mediated p21 gene expression in RMC, partly through HMT SET7/9 occupancy, suggesting H3Kme and SET7/9 may be potential renoprotective agents in managing chronic renal diseases.
Title: Histone Lysine Methylation in TGF-?1 Mediated p21 Gene Expression in Rat Mesangial Cells.

Study provided evidence of antiapoptotic effect of miR-30b in early phase of rat myocardial I/R injury model via the Ras/Akt pathway and showed KRAS as a target involved in miR-30b mediated anti-apoptotic effect.
Title: Anti-apoptotic effect of microRNA-30b in early phase of rat myocardial ischemia-reperfusion injury model.

VEGF/VEGFR/K-ras signaling pathway might promote the occurrence and development of hepatocellular carcinoma cells through regulating expression of miRNA21, which has potential clinical value for the development of therapies for hepatocellular carcinoma
Title: Effects of VEGF/VEGFR/K-ras signaling pathways on miRNA21 levels in hepatocellular carcinoma tissues in rats.

Findings demonstrated that oncogenic K-Ras activates the signaling cascade p38/PDPK1/PKCdelta/p47(phox)/NOX1 for ROS generation and consequent malignant cellular transformation.
Title: Novel signaling axis for ROS generation during K-Ras-induced cellular transformation.

The following Kras gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kras cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12710	NM_031515.3 Latest version!	Rattus norvegicus KRAS proto-oncogene, GTPase (Kras), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa12710	XM_008763354.2 Latest version!	Rattus norvegicus Kirsten rat sarcoma viral oncogene (Kras), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa27908	XM_017592442.1 Latest version!	Rattus norvegicus Kirsten rat sarcoma viral oncogene (Kras), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa27908	XM_017592443.1 Latest version!	Rattus norvegicus Kirsten rat sarcoma viral oncogene (Kras), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa42918	XM_008763355.2 Latest version!	Rattus norvegicus Kirsten rat sarcoma viral oncogene (Kras), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kras ( NM_031515.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113703.1 Rattus norvegicus KRAS proto-oncogene, GTPase (Kras), mRNA.

Price & Availability↑

CloneID	ORa12710
Clone ID Related Accession (Same CDS sequence)	XM_008763354.2 , NM_031515.3
Accession Version	NM_031515.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	GTPase KRas
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U09793.1. On Jun 10, 2007 this sequence version replaced NM_031515.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U09793.1, BC126086.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906349, SAMN01906351 [ECO:0000348] ##Evidence-Data-END##

ATGACTGAGT ATAAACTTGT GGTAGTTGGA GCTGGTGGCG TAGGCAAGAG TGCCTTGACG 
ATACAGCTAA TTCAGAATCA CTTTGTGGAT GAATATGATC CTACGATAGA GGACTCCTAC 
AGGAAACAAG TAGTAATTGA TGGAGAAACC TGTCTCTTGG ATATTCTCGA CACAGCAGGT 
CAAGAGGAGT ACAGTGCAAT GAGGGACCAG TACATGAGAA CTGGGGAGGG CTTTCTTTGT 
GTATTTGCCA TAAATAATAC TAAATCATTT GAAGATATTC ACCATTATAG AGAACAAATT 
AAAAGAGTAA AGGACTCTGA AGATGTGCCT ATGGTCCTAG TAGGGAATAA GTGTGACTTG 
CCTTCTAGAA CAGTAGACAC GAAACAGGCT CAGGAGTTAG CAAGGAGTTA TGGGATTCCA 
TTCATTGAGA CCTCAGCGAA GACAAGACAG GGTGTTGACG ATGCCTTCTA TACATTAGTC 
CGAGAAATTC GAAAACATAA AGAAAAGATG AGCAAAGATG GGAAAAAGAA GAAGAAGAAG 
TCAAGGACAA GGTGTATAGT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113703.1
CDS	1..567
Translation	MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQELARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSRTRCIVM

Target ORF information:

RefSeq Version	NM_031515.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus KRAS proto-oncogene, GTPase (Kras), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031515.3

ORF Insert Sequence:

ATGACTGAGT ATAAACTTGT GGTAGTTGGA GCTGGTGGCG TAGGCAAGAG TGCCTTGACG 
ATACAGCTAA TTCAGAATCA CTTTGTGGAT GAATATGATC CTACGATAGA GGACTCCTAC 
AGGAAACAAG TAGTAATTGA TGGAGAAACC TGTCTCTTGG ATATTCTCGA CACAGCAGGT 
CAAGAGGAGT ACAGTGCAAT GAGGGACCAG TACATGAGAA CTGGGGAGGG CTTTCTTTGT 
GTATTTGCCA TAAATAATAC TAAATCATTT GAAGATATTC ACCATTATAG AGAACAAATT 
AAAAGAGTAA AGGACTCTGA AGATGTGCCT ATGGTCCTAG TAGGGAATAA GTGTGACTTG 
CCTTCTAGAA CAGTAGACAC GAAACAGGCT CAGGAGTTAG CAAGGAGTTA TGGGATTCCA 
TTCATTGAGA CCTCAGCGAA GACAAGACAG GGTGTTGACG ATGCCTTCTA TACATTAGTC 
CGAGAAATTC GAAAACATAA AGAAAAGATG AGCAAAGATG GGAAAAAGAA GAAGAAGAAG 
TCAAGGACAA GGTGTATAGT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kras ( XM_008763354.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761576.1 Rattus norvegicus Kirsten rat sarcoma viral oncogene (Kras), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa12710
Clone ID Related Accession (Same CDS sequence)	XM_008763354.2 , NM_031515.3
Accession Version	XM_008763354.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	GTPase KRas isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAGT ATAAACTTGT GGTAGTTGGA GCTGGTGGCG TAGGCAAGAG TGCCTTGACG 
ATACAGCTAA TTCAGAATCA CTTTGTGGAT GAATATGATC CTACGATAGA GGACTCCTAC 
AGGAAACAAG TAGTAATTGA TGGAGAAACC TGTCTCTTGG ATATTCTCGA CACAGCAGGT 
CAAGAGGAGT ACAGTGCAAT GAGGGACCAG TACATGAGAA CTGGGGAGGG CTTTCTTTGT 
GTATTTGCCA TAAATAATAC TAAATCATTT GAAGATATTC ACCATTATAG AGAACAAATT 
AAAAGAGTAA AGGACTCTGA AGATGTGCCT ATGGTCCTAG TAGGGAATAA GTGTGACTTG 
CCTTCTAGAA CAGTAGACAC GAAACAGGCT CAGGAGTTAG CAAGGAGTTA TGGGATTCCA 
TTCATTGAGA CCTCAGCGAA GACAAGACAG GGTGTTGACG ATGCCTTCTA TACATTAGTC 
CGAGAAATTC GAAAACATAA AGAAAAGATG AGCAAAGATG GGAAAAAGAA GAAGAAGAAG 
TCAAGGACAA GGTGTATAGT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761576.1
CDS	57..623
Translation	MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQELARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSRTRCIVM

Target ORF information:

RefSeq Version	XM_008763354.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Kirsten rat sarcoma viral oncogene (Kras), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763354.2

ORF Insert Sequence:

ATGACTGAGT ATAAACTTGT GGTAGTTGGA GCTGGTGGCG TAGGCAAGAG TGCCTTGACG 
ATACAGCTAA TTCAGAATCA CTTTGTGGAT GAATATGATC CTACGATAGA GGACTCCTAC 
AGGAAACAAG TAGTAATTGA TGGAGAAACC TGTCTCTTGG ATATTCTCGA CACAGCAGGT 
CAAGAGGAGT ACAGTGCAAT GAGGGACCAG TACATGAGAA CTGGGGAGGG CTTTCTTTGT 
GTATTTGCCA TAAATAATAC TAAATCATTT GAAGATATTC ACCATTATAG AGAACAAATT 
AAAAGAGTAA AGGACTCTGA AGATGTGCCT ATGGTCCTAG TAGGGAATAA GTGTGACTTG 
CCTTCTAGAA CAGTAGACAC GAAACAGGCT CAGGAGTTAG CAAGGAGTTA TGGGATTCCA 
TTCATTGAGA CCTCAGCGAA GACAAGACAG GGTGTTGACG ATGCCTTCTA TACATTAGTC 
CGAGAAATTC GAAAACATAA AGAAAAGATG AGCAAAGATG GGAAAAAGAA GAAGAAGAAG 
TCAAGGACAA GGTGTATAGT CATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kras ( XM_017592443.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447932.1 Rattus norvegicus Kirsten rat sarcoma viral oncogene (Kras), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa27908
Clone ID Related Accession (Same CDS sequence)	XM_017592443.1 , XM_017592442.1
Accession Version	XM_017592443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	GTPase KRas isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAGT ATAAACTTGT GGTAGTTGGA GCTGGTGGCG TAGGCAAGAG TGCCTTGACG 
ATACAGCTAA TTCAGAATCA CTTTGTGGAT GAATATGATC CTACGATAGA GGACTCCTAC 
AGGAAACAAG TAGTAATTGA TGGAGAAACC TGTCTCTTGG ATATTCTCGA CACAGCAGGT 
CAAGAGGAGT ACAGTGCAAT GAGGGACCAG TACATGAGAA CTGGGGAGGG CTTTCTTTGT 
GTATTTGCCA TAAATAATAC TAAATCATTT GAAGATATTC ACCATTATAG AGAACAAATT 
AAAAGAGTAA AGGACTCTGA AGATGTGCCT ATGGTCCTAG TAGGGAATAA GTGTGACTTG 
CCTTCTAGAA CAGTAGACAC GAAACAGGCT CAGGAGTTAG CAAGGAGTTA TGGGATTCCA 
TTCATTGAGA CCTCAGCGAA GACAAGACAG AGAGTGGAGG ATGCTTTTTA TACATTGGTG 
AGAGAGATCC GACAGTACAG ATTGAAAAAA ATCAGCAAAG AAGAAAAGAC TCCTGGCTGT 
GTGAAAATTA AAAAATGCGT TATAATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447932.1
CDS	194..763
Translation	MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQELARSYGIPFIETSAKTRQRVEDAFYTLVREIRQYRLKKISKEEKTPGCVKIKKCVIM

Target ORF information:

RefSeq Version	XM_017592443.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Kirsten rat sarcoma viral oncogene (Kras), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592443.1

ORF Insert Sequence:

ATGACTGAGT ATAAACTTGT GGTAGTTGGA GCTGGTGGCG TAGGCAAGAG TGCCTTGACG 
ATACAGCTAA TTCAGAATCA CTTTGTGGAT GAATATGATC CTACGATAGA GGACTCCTAC 
AGGAAACAAG TAGTAATTGA TGGAGAAACC TGTCTCTTGG ATATTCTCGA CACAGCAGGT 
CAAGAGGAGT ACAGTGCAAT GAGGGACCAG TACATGAGAA CTGGGGAGGG CTTTCTTTGT 
GTATTTGCCA TAAATAATAC TAAATCATTT GAAGATATTC ACCATTATAG AGAACAAATT 
AAAAGAGTAA AGGACTCTGA AGATGTGCCT ATGGTCCTAG TAGGGAATAA GTGTGACTTG 
CCTTCTAGAA CAGTAGACAC GAAACAGGCT CAGGAGTTAG CAAGGAGTTA TGGGATTCCA 
TTCATTGAGA CCTCAGCGAA GACAAGACAG AGAGTGGAGG ATGCTTTTTA TACATTGGTG 
AGAGAGATCC GACAGTACAG ATTGAAAAAA ATCAGCAAAG AAGAAAAGAC TCCTGGCTGT 
GTGAAAATTA AAAAATGCGT TATAATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kras ( XM_017592442.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447931.1 Rattus norvegicus Kirsten rat sarcoma viral oncogene (Kras), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa27908
Clone ID Related Accession (Same CDS sequence)	XM_017592443.1 , XM_017592442.1
Accession Version	XM_017592442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	GTPase KRas isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAGT ATAAACTTGT GGTAGTTGGA GCTGGTGGCG TAGGCAAGAG TGCCTTGACG 
ATACAGCTAA TTCAGAATCA CTTTGTGGAT GAATATGATC CTACGATAGA GGACTCCTAC 
AGGAAACAAG TAGTAATTGA TGGAGAAACC TGTCTCTTGG ATATTCTCGA CACAGCAGGT 
CAAGAGGAGT ACAGTGCAAT GAGGGACCAG TACATGAGAA CTGGGGAGGG CTTTCTTTGT 
GTATTTGCCA TAAATAATAC TAAATCATTT GAAGATATTC ACCATTATAG AGAACAAATT 
AAAAGAGTAA AGGACTCTGA AGATGTGCCT ATGGTCCTAG TAGGGAATAA GTGTGACTTG 
CCTTCTAGAA CAGTAGACAC GAAACAGGCT CAGGAGTTAG CAAGGAGTTA TGGGATTCCA 
TTCATTGAGA CCTCAGCGAA GACAAGACAG AGAGTGGAGG ATGCTTTTTA TACATTGGTG 
AGAGAGATCC GACAGTACAG ATTGAAAAAA ATCAGCAAAG AAGAAAAGAC TCCTGGCTGT 
GTGAAAATTA AAAAATGCGT TATAATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447931.1
CDS	57..626
Translation	MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQELARSYGIPFIETSAKTRQRVEDAFYTLVREIRQYRLKKISKEEKTPGCVKIKKCVIM

Target ORF information:

RefSeq Version	XM_017592442.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Kirsten rat sarcoma viral oncogene (Kras), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592442.1

ORF Insert Sequence:

ATGACTGAGT ATAAACTTGT GGTAGTTGGA GCTGGTGGCG TAGGCAAGAG TGCCTTGACG 
ATACAGCTAA TTCAGAATCA CTTTGTGGAT GAATATGATC CTACGATAGA GGACTCCTAC 
AGGAAACAAG TAGTAATTGA TGGAGAAACC TGTCTCTTGG ATATTCTCGA CACAGCAGGT 
CAAGAGGAGT ACAGTGCAAT GAGGGACCAG TACATGAGAA CTGGGGAGGG CTTTCTTTGT 
GTATTTGCCA TAAATAATAC TAAATCATTT GAAGATATTC ACCATTATAG AGAACAAATT 
AAAAGAGTAA AGGACTCTGA AGATGTGCCT ATGGTCCTAG TAGGGAATAA GTGTGACTTG 
CCTTCTAGAA CAGTAGACAC GAAACAGGCT CAGGAGTTAG CAAGGAGTTA TGGGATTCCA 
TTCATTGAGA CCTCAGCGAA GACAAGACAG AGAGTGGAGG ATGCTTTTTA TACATTGGTG 
AGAGAGATCC GACAGTACAG ATTGAAAAAA ATCAGCAAAG AAGAAAAGAC TCCTGGCTGT 
GTGAAAATTA AAAAATGCGT TATAATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kras ( XM_008763355.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761577.1 Rattus norvegicus Kirsten rat sarcoma viral oncogene (Kras), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa42918
Clone ID Related Accession (Same CDS sequence)	XM_008763355.2
Accession Version	XM_008763355.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	GTPase KRas isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGAGT ATAAACTTGT GGTAGTTGGA GCTGGTGGCG TAGGCAAGAG TGCCTTGACG 
ATACAGCTAA TTCAGAATCA CTTTGTGGAT GAATATGATC CTACGATAGA GGACTCCTAC 
AGGAAACAAG TAGTAATTGA TGGAGAAACC TGTCTCTTGG ATATTCTCGA CACAGCAGGT 
CAAGAGGAGT ACAGTGCAAT GAGGGACCAG TACATGAGAA CTGGGGAGGG CTTTCTTTGT 
GTATTTGCCA TAAATAATAC TAAATCATTT GAAGATATTC ACCATTATAG AGAACAAATT 
AAAAGAGTAA AGGACTCTGA AGATGTGCCT ATGGTCCTAG TAGGGAATAA GTGTGACTTG 
CCTTCTAGAA CAGTAGACAC GAAACAGGCT CAGGAGTTAG CAAGGAGTTA TGGGATTCCA 
TTCATTGAGA CCTCAGCGAA GACAAGACAG AGAGTGGAGG ATGCTTTTTA TACATTGGTG 
AGAGAGATCC GACAGTACAG ATTGAAAAAA ATCAGCAAAG AAGAAAAGAC TCCTGGCTGT 
GTGAAAATTA AAAAATGCGT TATAATGGGT GTTGACGATG CCTTCTATAC ATTAGTCCGA 
GAAATTCGAA AACATAAAGA AAAGATGAGC AAAGATGGGA AAAAGAAGAA GAAGAAGTCA 
AGGACAAGGT GTATAGTCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761577.1
CDS	2594..3277
Translation	MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQELARSYGIPFIETSAKTRQRVEDAFYTLVREIRQYRLKKISKEEKTPGCVKIKKCVIMGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSRTRCIVM

Target ORF information:

RefSeq Version	XM_008763355.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Kirsten rat sarcoma viral oncogene (Kras), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763355.2

ORF Insert Sequence:

ATGACTGAGT ATAAACTTGT GGTAGTTGGA GCTGGTGGCG TAGGCAAGAG TGCCTTGACG 
ATACAGCTAA TTCAGAATCA CTTTGTGGAT GAATATGATC CTACGATAGA GGACTCCTAC 
AGGAAACAAG TAGTAATTGA TGGAGAAACC TGTCTCTTGG ATATTCTCGA CACAGCAGGT 
CAAGAGGAGT ACAGTGCAAT GAGGGACCAG TACATGAGAA CTGGGGAGGG CTTTCTTTGT 
GTATTTGCCA TAAATAATAC TAAATCATTT GAAGATATTC ACCATTATAG AGAACAAATT 
AAAAGAGTAA AGGACTCTGA AGATGTGCCT ATGGTCCTAG TAGGGAATAA GTGTGACTTG 
CCTTCTAGAA CAGTAGACAC GAAACAGGCT CAGGAGTTAG CAAGGAGTTA TGGGATTCCA 
TTCATTGAGA CCTCAGCGAA GACAAGACAG AGAGTGGAGG ATGCTTTTTA TACATTGGTG 
AGAGAGATCC GACAGTACAG ATTGAAAAAA ATCAGCAAAG AAGAAAAGAC TCCTGGCTGT 
GTGAAAATTA AAAAATGCGT TATAATGGGT GTTGACGATG CCTTCTATAC ATTAGTCCGA 
GAAATTCGAA AACATAAAGA AAAGATGAGC AAAGATGGGA AAAAGAAGAA GAAGAAGTCA 
AGGACAAGGT GTATAGTCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Wild type Kirsten rat sarcoma is a novel microRNA-622-regulated therapeutic target for hepatocellular carcinoma and contributes to sorafenib resistance.
Gut(2017 Dec)
Dietrich P,Koch A,Fritz V,Hartmann A,Bosserhoff AK,Hellerbrand C

The ALPPS Approach for Colorectal Liver Metastases: Impact of KRAS Mutation Status in Survival.
Digestive surgery(2017 Oct)
Serenari M,Alvarez FA,Ardiles V,de Santiba?es M,Pekolj J,de Santiba?es E

Mucinous cystic neoplasms of the liver and pancreas: relationship between KRAS driver mutations and disease progression.
Histopathology71(4)591-600(2017 Oct)
Fujikura K,Akita M,Abe-Suzuki S,Itoh T,Zen Y

Activating KRAS mutation is prognostic only among patients who receive preoperative chemotherapy before resection of colorectal liver metastases.
Journal of surgical oncology114(3)361-7(2016 Sep)
Margonis GA,Kim Y,Sasaki K,Samaha M,Buettner S,Amini N,Pawlik TM

Nucleotide sequence of exon I of the rat c-K-ras gene.
The Bulletin of Tokyo Medical and Dental University33(1)35-40(1986 Mar)
Iritani A,Katayama N,Tahira T,Hayashi K,Tsuchida N

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Kras cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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