Ras85D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ras85D
Entrez Gene ID	41140
Full Name	Ras oncogene at 85D
Synonyms	C-ras1,CG9375,D-Ras,D-Ras1,D-ras-1,D-ras1,DRAS1,DRas,DRas85D/Ras,Dm Ras1,Dmel\CG9375,Dmras85D,Dras,Dras1,Dras85D,E(faf),E(sev)3C,EC3-3,EK3-4,P08646,RAS,RAS1,RAS85D,RTK,Ras,Ras 85D,Ras-1,Ras1,Ras1/RAs85D,Ras185D,Ras85,Ras85DNt,RasI,RasV12,Ras[V12],S35097,Su(tor)3-2,dRAS1,dRas,dRas1,dRas85D,dras1,fs(3)05703,l(3)06677,l(3)s1747,p21[Ras1],ras,ras 1,ras1,ras85B,ras85D
General protein information	Preferred Names Ras oncogene at 85D Names CG9375 gene product from transcript CG9375-RA CG9375-PA Ras GTPase Ras oncogene at 85B Ras1 Ras85D-PA enhancer of faf
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85D19-85D19\|3-50 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057351.5	NP_476699.1	Ras oncogene at 85D

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	KRAS	XP_003433608.2
Homo sapiens (human)	KRAS	NP_203524.1
Drosophila melanogaster (fruit fly)	Ras85D	NP_476699.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002219	XP_307965.4
Xenopus tropicalis (tropical clawed frog)	LOC100485959	XP_004920180.1
Pan troglodytes (chimpanzee)	KRAS	XP_003313842.1
Mus musculus (house mouse)	Kras	NP_067259.4
Caenorhabditis elegans (roundworm)	let-60	NP_502213.3
Bos taurus (cattle)	KRAS	NP_001103471.1
Rattus norvegicus (Norway rat)	Kras	NP_113703.1
Gallus gallus (chicken)	KRAS	NP_001243091.1
Danio rerio (zebrafish)	kras	NP_001003744.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thus, our current work provides a novel mechanism of pupal size determination by microRNAs during Drosophila melanogaster metamorphosis.
Title: miR-11 regulates pupal size of Drosophila melanogaster via directly targeting Ras85D.

Oncogenic Ras stimulates Eiger/TNF exocytosis to promote growth.
Title: Oncogenic Ras stimulates Eiger/TNF exocytosis to promote growth.

Data indicate that dysfunction of Rab5 leads to cell-autonomous accumulation of a TNF homolog Eiger, and causes activation of Ras signaling.
Title: Loss of Rab5 drives non-autonomous cell proliferation through TNF and Ras signaling in Drosophila.

According to Bridges' map, all three bands (Dras1, Dras2, Dras3) were mapped to regions 85D, 64B, and 62B of chromosome 3. Among them, only Drasl has a common origin with ras genes of mammals.
Title: [Universal intracellular transducer RAS and its role in the development of Drosophila].

RNAi-mediated inhibition of MAPK-signaling by knockdown of Ras85D, phl/D-Raf or Dsor1/MEK affects larval development, adult life span and hemolymph phosphate, suggesting that some in vivo effects involve activation of this signaling pathway by phosphate.
Title: Genetic determinants of phosphate response in Drosophila.

The following Ras85D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ras85D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08217	NM_057351.5 Latest version!	Drosophila melanogaster Ras oncogene at 85D (Ras85D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ras85D ( NM_057351.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476699.1 Drosophila melanogaster Ras oncogene at 85D (Ras85D), mRNA.

Price & Availability↑

CloneID	OFa08217
Clone ID Related Accession (Same CDS sequence)	NM_057351.5
Accession Version	NM_057351.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ras oncogene at 85D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057351.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGAAT ACAAACTGGT CGTCGTTGGA GCCGGAGGCG TGGGCAAGTC CGCGCTCACC 
ATCCAGCTAA TCCAGAACCA TTTCGTGGAC GAGTACGACC CCACAATCGA GGACTCTTAC 
CGAAAGCAAG TGGTTATCGA TGGAGAGACC TGCCTGCTGG ACATCCTGGA CACCGCCGGC 
CAAGAGGAGT ACTCGGCCAT GCGGGATCAG TATATGCGGA CTGGCGAGGG ATTCCTGCTG 
GTCTTTGCCG TCAACAGTGC GAAGTCCTTC GAGGATATCG GCACCTACCG TGAGCAGATC 
AAGCGCGTAA AGGATGCCGA AGAGGTGCCC ATGGTGCTGG TGGGCAACAA ATGTGATCTG 
GCCTCGTGGA ACGTTAACAA CGAGCAGGCA AGAGAGGTGG CCAAACAGTA CGGCATTCCA 
TACATTGAGA CATCCGCCAA GACGCGCATG GGCGTGGACG ATGCATTTTA CACACTGGTG 
CGCGAAATCC GCAAGGACAA GGACAACAAG GGGCGGAGGG GCCGCAAAAT GAACAAGCCG 
AATCGTAGAT TTAAATGTAA AATGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476699.1
CDS	442..1011
Translation	MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLLVFAVNSAKSFEDIGTYREQIKRVKDAEEVPMVLVGNKCDLASWNVNNEQAREVAKQYGIPYIETSAKTRMGVDDAFYTLVREIRKDKDNKGRRGRKMNKPNRRFKCKML

Target ORF information:

RefSeq Version	NM_057351.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ras oncogene at 85D (Ras85D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057351.5

ORF Insert Sequence:

ATGACGGAAT ACAAACTGGT CGTCGTTGGA GCCGGAGGCG TGGGCAAGTC CGCGCTCACC 
ATCCAGCTAA TCCAGAACCA TTTCGTGGAC GAGTACGACC CCACAATCGA GGACTCTTAC 
CGAAAGCAAG TGGTTATCGA TGGAGAGACC TGCCTGCTGG ACATCCTGGA CACCGCCGGC 
CAAGAGGAGT ACTCGGCCAT GCGGGATCAG TATATGCGGA CTGGCGAGGG ATTCCTGCTG 
GTCTTTGCCG TCAACAGTGC GAAGTCCTTC GAGGATATCG GCACCTACCG TGAGCAGATC 
AAGCGCGTAA AGGATGCCGA AGAGGTGCCC ATGGTGCTGG TGGGCAACAA ATGTGATCTG 
GCCTCGTGGA ACGTTAACAA CGAGCAGGCA AGAGAGGTGG CCAAACAGTA CGGCATTCCA 
TACATTGAGA CATCCGCCAA GACGCGCATG GGCGTGGACG ATGCATTTTA CACACTGGTG 
CGCGAAATCC GCAAGGACAA GGACAACAAG GGGCGGAGGG GCCGCAAAAT GAACAAGCCG 
AATCGTAGAT TTAAATGTAA AATGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Ras85D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報