let-60 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	let-60
Entrez Gene ID	178104
Full Name	Ras protein let-60
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_069812.3	NP_502213.3	Ras protein let-60
NM_069812.4	NP_502213.3	Ras protein let-60

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	KRAS	XP_003433608.2
Homo sapiens (human)	KRAS	NP_203524.1
Drosophila melanogaster (fruit fly)	Ras85D	NP_476699.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002219	XP_307965.4
Xenopus tropicalis (tropical clawed frog)	LOC100485959	XP_004920180.1
Pan troglodytes (chimpanzee)	KRAS	XP_003313842.1
Mus musculus (house mouse)	Kras	NP_067259.4
Caenorhabditis elegans (roundworm)	let-60	NP_502213.3
Bos taurus (cattle)	KRAS	NP_001103471.1
Rattus norvegicus (Norway rat)	Kras	NP_113703.1
Gallus gallus (chicken)	KRAS	NP_001243091.1
Danio rerio (zebrafish)	kras	NP_001003744.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression of activated LET-60/Ras in the neighboring duct cell, but not in the G1 or G2 cells, is sufficient to rescue sos-1 delamination defects, revealing that Ras acts non-cell-autonomously to permit G1 delamination.
Title: A non-cell-autonomous role for Ras signaling in C. elegans neuroblast delamination.

BET-1 as a Sumo-dependent attenuator of LET-60-mediated signalling and our characterisation suggests that BET-1 can be sumoylated.
Title: An RNAi-based dimorphic genetic screen identified the double bromodomain protein BET-1 as a sumo-dependent attenuator of RAS-mediated signalling.

Canal cell asymmetry and let-60/Ras signaling influence which of two equivalent precursors will attach to the canal cell.
Title: Notch and Ras promote sequential steps of excretory tube development in C. elegans.

LET-60 (Ras) is identified as a critical RGEF-1b substrate-effector in sensory neurons.
Title: A RasGRP, C. elegans RGEF-1b, couples external stimuli to behavior by activating LET-60 (Ras) in sensory neurons.

Goa-1 Goalpha and let-60 Ras act in parallel to gpc-1 in olfactory pathway.
Title: GPC-1, a G protein gamma-subunit, regulates olfactory adaptation in Caenorhabditis elegans.

The following let-60 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the let-60 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa344412	NM_069812.4 Latest version!	Caenorhabditis elegans Ras protein let-60 (let-60), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OCa344412	NM_069812.3	Caenorhabditis elegans Ras protein let-60 (let-60), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

let-60 ( NM_069812.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_502213.3 Caenorhabditis elegans Ras protein let-60 (let-60), partial mRNA.

Price & Availability↑

CloneID	OCa344412
Clone ID Related Accession (Same CDS sequence)	NM_069812.3 , NM_069812.4
Accession Version	NM_069812.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Ras protein let-60
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_069812.2. COMPLETENESS: incomplete on both ends.

ATGACGGAGT ACAAGCTTGT GGTAGTTGGA GATGGAGGAG TTGGTAAATC AGCACTCACC 
ATTCAACTCA TCCAGAATCA CTTTGTCGAA GAATACGACC CGACCATAGA GGACAGCTAC 
AGAAAGCAGG TTGTGATAGA CGGTGAGACA TGCCTCCTCG ACATATTGGA TACCGCCGGA 
CAAGAAGAAT ATTCGGCGAT GCGTGATCAG TACATGAGGA CAGGCGAAGG ATTTCTGTTG 
GTTTTCGCCG TCAACGAGGC TAAATCTTTC GAGAATGTCG CTAACTACCG CGAGCAGATT 
CGGAGGGTAA AGGATTCAGA TGATGTTCCT ATGGTCTTGG TAGGGAATAA ATGTGATTTG 
TCATCTCGAT CAGTCGACTT CCGAACAGTC AGTGAGACAG CAAAGGGTTA CGGTATTCCG 
AATGTCGACA CATCTGCCAA AACGCGTATG GGAGTTGATG AAGCATTTTA CACACTTGTT 
AGAGAAATTC GCAAGCATCG TGAGCGTCAC GACAATAATA AGCCACAAAA GAAGAAGAAG 
TGTCAAATAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_502213.3
CDS	1..555
Translation	MTEYKLVVVGDGGVGKSALTIQLIQNHFVEEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLLVFAVNEAKSFENVANYREQIRRVKDSDDVPMVLVGNKCDLSSRSVDFRTVSETAKGYGIPNVDTSAKTRMGVDEAFYTLVREIRKHRERHDNNKPQKKKKCQIM

Target ORF information:

RefSeq Version	NM_069812.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Ras protein let-60 (let-60), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_069812.3

ORF Insert Sequence:

ATGACGGAGT ACAAGCTTGT GGTAGTTGGA GATGGAGGAG TTGGTAAATC AGCACTCACC 
ATTCAACTCA TCCAGAATCA CTTTGTCGAA GAATACGACC CGACCATAGA GGACAGCTAC 
AGAAAGCAGG TTGTGATAGA CGGTGAGACA TGCCTCCTCG ACATATTGGA TACCGCCGGA 
CAAGAAGAAT ATTCGGCGAT GCGTGATCAG TACATGAGGA CAGGCGAAGG ATTTCTGTTG 
GTTTTCGCCG TCAACGAGGC TAAATCTTTC GAGAATGTCG CTAACTACCG CGAGCAGATT 
CGGAGGGTAA AGGATTCAGA TGATGTTCCT ATGGTCTTGG TAGGGAATAA ATGTGATTTG 
TCATCTCGAT CAGTCGACTT CCGAACAGTC AGTGAGACAG CAAAGGGTTA CGGTATTCCG 
AATGTCGACA CATCTGCCAA AACGCGTATG GGAGTTGATG AAGCATTTTA CACACTTGTT 
AGAGAAATTC GCAAGCATCG TGAGCGTCAC GACAATAATA AGCCACAAAA GAAGAAGAAG 
TGTCAAATAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

let-60 ( NM_069812.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_502213.3 Caenorhabditis elegans Ras protein let-60 (let-60), mRNA.

Price & Availability↑

CloneID	OCa344412
Clone ID Related Accession (Same CDS sequence)	NM_069812.3 , NM_069812.4
Accession Version	NM_069812.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Ras protein let-60
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_069812.3.

ATGACGGAGT ACAAGCTTGT GGTAGTTGGA GATGGAGGAG TTGGTAAATC AGCACTCACC 
ATTCAACTCA TCCAGAATCA CTTTGTCGAA GAATACGACC CGACCATAGA GGACAGCTAC 
AGAAAGCAGG TTGTGATAGA CGGTGAGACA TGCCTCCTCG ACATATTGGA TACCGCCGGA 
CAAGAAGAAT ATTCGGCGAT GCGTGATCAG TACATGAGGA CAGGCGAAGG ATTTCTGTTG 
GTTTTCGCCG TCAACGAGGC TAAATCTTTC GAGAATGTCG CTAACTACCG CGAGCAGATT 
CGGAGGGTAA AGGATTCAGA TGATGTTCCT ATGGTCTTGG TAGGGAATAA ATGTGATTTG 
TCATCTCGAT CAGTCGACTT CCGAACAGTC AGTGAGACAG CAAAGGGTTA CGGTATTCCG 
AATGTCGACA CATCTGCCAA AACGCGTATG GGAGTTGATG AAGCATTTTA CACACTTGTT 
AGAGAAATTC GCAAGCATCG TGAGCGTCAC GACAATAATA AGCCACAAAA GAAGAAGAAG 
TGTCAAATAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_502213.3
CDS	14..568
Translation	MTEYKLVVVGDGGVGKSALTIQLIQNHFVEEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLLVFAVNEAKSFENVANYREQIRRVKDSDDVPMVLVGNKCDLSSRSVDFRTVSETAKGYGIPNVDTSAKTRMGVDEAFYTLVREIRKHRERHDNNKPQKKKKCQIM

Target ORF information:

RefSeq Version	NM_069812.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Ras protein let-60 (let-60), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_069812.4

ORF Insert Sequence:

ATGACGGAGT ACAAGCTTGT GGTAGTTGGA GATGGAGGAG TTGGTAAATC AGCACTCACC 
ATTCAACTCA TCCAGAATCA CTTTGTCGAA GAATACGACC CGACCATAGA GGACAGCTAC 
AGAAAGCAGG TTGTGATAGA CGGTGAGACA TGCCTCCTCG ACATATTGGA TACCGCCGGA 
CAAGAAGAAT ATTCGGCGAT GCGTGATCAG TACATGAGGA CAGGCGAAGG ATTTCTGTTG 
GTTTTCGCCG TCAACGAGGC TAAATCTTTC GAGAATGTCG CTAACTACCG CGAGCAGATT 
CGGAGGGTAA AGGATTCAGA TGATGTTCCT ATGGTCTTGG TAGGGAATAA ATGTGATTTG 
TCATCTCGAT CAGTCGACTT CCGAACAGTC AGTGAGACAG CAAAGGGTTA CGGTATTCCG 
AATGTCGACA CATCTGCCAA AACGCGTATG GGAGTTGATG AAGCATTTTA CACACTTGTT 
AGAGAAATTC GCAAGCATCG TGAGCGTCAC GACAATAATA AGCCACAAAA GAAGAAGAAG 
TGTCAAATAA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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let-60 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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