HECW1 gene cDNA ORF clone, Macaca mulatta(Rhesus monkey)

mRNA	Protein	Name
XM_015133526.1	XP_014989012.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_015133533.1	XP_014989019.1	E3 ubiquitin-protein ligase HECW1 isoform X6
XM_028844862.1	XP_028700695.1	E3 ubiquitin-protein ligase HECW1 isoform X4
XM_015133529.1	XP_014989015.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_015133531.1	XP_014989017.1	E3 ubiquitin-protein ligase HECW1 isoform X4
NM_001265810.1	NP_001252739.1	E3 ubiquitin-protein ligase HECW1
XM_015133529.2	XP_014989015.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_015133531.2	XP_014989017.1	E3 ubiquitin-protein ligase HECW1 isoform X5
XM_015133532.2	XP_014989018.1	E3 ubiquitin-protein ligase HECW1 isoform X6
XM_015133527.1	XP_014989013.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_015133534.1	XP_014989020.1	E3 ubiquitin-protein ligase HECW1 isoform X7
XM_015133530.2	XP_014989016.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_028844861.1	XP_028700694.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_028844863.1	XP_028700696.1	E3 ubiquitin-protein ligase HECW1 isoform X8
XM_015133530.1	XP_014989016.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_015133532.1	XP_014989018.1	E3 ubiquitin-protein ligase HECW1 isoform X5
XM_015133526.2	XP_014989012.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_015133527.2	XP_014989013.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_015133533.2	XP_014989019.1	E3 ubiquitin-protein ligase HECW1 isoform X7
XM_015133534.2	XP_014989020.1	E3 ubiquitin-protein ligase HECW1 isoform X9

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMb81465	XM_028844863.1 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMb81458	XM_015133526.2 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMb81459	XM_015133527.2 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMb81460	XM_015133530.2 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMb81460	XM_028844861.1 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMb81460	XM_015133529.2 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMb81461	XM_028844862.1 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMb81462	XM_015133531.2 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMb81463	XM_015133532.2 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMb81464	XM_015133533.2 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMb03739	NM_001265810.1 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OMb30252	XM_015133526.1 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OMb30253	XM_015133527.1 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OMb30254	XM_015133530.1 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OMb30254	XM_015133529.1 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OMb30255	XM_015133531.1 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OMb30256	XM_015133532.1 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OMb30257	XM_015133533.1 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OMb30258	XM_015133534.2 Latest version!	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
View Old Accession Versions >>
OMb30258	XM_015133534.1	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
Hide Old Accession Versions >>

HECW1 ( XM_028844863.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028700696.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMb81465
Clone ID Related Accession (Same CDS sequence)	XM_028844863.1
Accession Version	XM_028844863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041756.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC TGGCAGAACC CAAAGATGAC TGCATTTCCA AGGAGGTCAA ATGTTCAGGA 
CAAGAACTGC AAATTCAAAA GCTTCCAGGG AGCCAGATTT TTAAAAGCAT CGGCGCTGAT 
GAGACTGTAC AAGGACAAGG AAGTCGGAGG CTGATCAGCT TCTCTCTCTC AGATTTCCAA 
GCCATGGGGT TGAAGAAAGG GATGTTTTTC AACCCAGACC CTTATCTGAA GATTTCCATT 
CAGCCTGGGA AGCACAGCAT CTTCCCCGCC CTCCCTCACC ATGGACAGGA GAGGAGATCC 
AAGATCATAG GCAACACCGT GAACCCCATC TGGCAGGCCG AGCAATTCAG TTTTGTGTCC 
TTGCCCACTG ACGTGCTGGA AATTGAGGTG AAGGACAAGT TTGCCAAGAG CCGTCCCATC 
ATCAAGCGTT TCTTGGGAAA GCTGTCGATG CCCGTTCAAA GACTCCTGGA GAGACACGCC 
ATAGGGGATA GGGTGGTCAG CTACACACTT GGCCGCAGGC TTCCAACAGA TCATGTGAGT 
GGACAGCTGC AATTCCGATT TGAGATCACT TCCTCCATCC ACCCAGCAGA TGATGAGGAG 
ATTTCCCTGA GTACGGAGCC TGAGTCAGCC CCCATTCAGG ACAGCCCCAT GAACAACCTG 
ATGGAAAGCG GCAGTGGGGA ACCTCGATCT GAGGCACCGG AGTCCTCTGA GGGCTGGAAG 
CCAGAGCAGC TGGGTGAGGG CAGTGTCCTG GATGGTCCAG GGAACCAAAG CATGGAGCTT 
TCCAGACCAG CTGAGGAAGC AGCAGTCATC ACGGAGGCAG GAGACCAGGG CATGGTCTCT 
GTGGGACCTG AAGGGGCTGG GGACCTCCTG GCCCAGGTGC AAAAGGACAT CCAGCCTGCC 
CCGAGTGCAG AAGAGCTGGC CGAGCAGCTG GACCTGGGTG AGGAGGCGTC AGCACTGCTG 
CTGGAAGACG GTGAAGCACC AGCCAGCACC GAGGAGGAGC CCTTAGAGGA GGAAGCAACG 
ACACAGAGCC GGGCTGGAAG GGAAGAAGAG AAGGAGCAGG AGGAGGAGGA GGGAGATGTG 
TCTACCCTGG AGCAGGGAGA GGGCAGGCTG CAACTGCGGG CCTCGGTGAA GAGAAAAAGC 
AGGCCCTGCT CCTTGCCTGT GTCTGAGCTG GAGACAGTGA TCGCGTCAGC CTGCGGGGAC 
CCCGAGACCC CGCGGACACA CTACATCCGC ATCCACACCC TGCTGCACAG CATGCCCTCG 
GCCCAGGGCG GCAGCGCGGC AGAGGAGGAG GACGGCGCGG AGGAGGAGTC CACCCTCAAG 
GACTCCTCGG AGAAGGATGG GCTCAGCGAG GTGGACACAG TGGCGGCTGA CCCGTCTGCC 
CTGGAAGAGG ACAGAGAAGA GCCCGAGGGG GCTACTCCAG GCACGGCGCA TCCTGGCCAC 
TCCGGGGGCC ACTTCTCCAG CCTGGCCAAT GGCGCCGCCC AGGATGGCGA CACGCACCCC 
AGCACCGGCA GCGAGAGCGA CTCCAGCCCC AGGCAAGGCG GGGACCACAG TTGCGAGGGC 
TGTGATGCGT CCTGCTGCAG CCCCTCGTGC TACAGCTCCT CGTGCTACAG CACGTCCTGC 
TACAGCAGCT CGTGCTACAG CGCCTCCTGC TACAGCCCCT CCTGCTACAA CGGCAACAGG 
TTCGCCAGCC ACACGCGCTT CTCCTCCGTA GACAGCGCCA AGATCTCCGA GAGCACGGTC 
TTCTCCTCGC AAGATGATGA GGAGGAGGAG AACAGCGCGT TCGAATCTGT GCCCGACTCC 
ATGCAGAGCC CTGAGCTGGA CCCGGAGTCC ACGAACGGCG CTGGGCCGTG GCAAGACGAG 
CTGGCCGCCC CTAGCGGGCA CGAGGAAAGA ACCCCGGAAG GTCTGGAATC CCCCGTGGCA 
GGTCCAAGCA ATCGGAGAGA AGGTGAATGT CCTATACTCC ATAATTCCCA GCCAGTAAGC 
CAGCTTCCTT CCCTGAGGCC TGAACATCAT CACTACCCAA CAATCGATGA GCCTCTTCCA 
CCAAACTGGG AAGCTCGAAT TGACAGCCAC GGGCGGGTCT TTTATGTGGA CCACGTGAAC 
CGCACAACCA CCTGGCAGCG TCCAACGGCA GCAGCCACCC CGGATGGCAT GCGGAGATCG 
GGGTCCATCC AGCAGATGGA GCAACTCAAC AGGCGGTATC AAAACATTCA GCGAACCATT 
GCAACAGAGA GGTCGGAAGA AGATTCTGGC AGCCAAAGCT GCGAGCAAGC CCCAGCAGGA 
GGAGGCGGAG GTGGAGGGAG TGACTCAGAA GCCGAATCTT CCCAGTCCAG CTTAGATCTA 
AGGAGAGAAG GGTCACTTTC TCCGGTTAAC TCACAAAAAA TCACCTTGCT GCTGCAGTCC 
CCAGCGGTCA AGTTCATCAC CAACCCCGAA TTCTTCACTG TGCTGCATGC CAATTATAGT 
GCCTACCGAG TGTTCACCAG TAGCACCTGC TTAAAGCACA TGATTCTGAA AGTCCGACGG 
GATGCTCGCA ATTTTGAACG CTACCAGCAC AACCGGGACT TGGTGAATTT CATCAACATG 
TTCGCAGACA CTCGGCTGGA ACTGCCCCGG GGCTGGGAGA TCAAAACGGA CCAGCAGGGA 
AAGTCTTTTT TCGTGGACCA CAACAGTCGA GCTACCACTT TCATTGACCC CCGAATCCCT 
CTTCAGAACG GTCGTCTTCC CAATCATCTA ACCCACCGAC AGCACCTCCA GAGACTCCGA 
AGTTACAGCG CTGGAGAGGC CTCAGAAGTT TCTAGAAACA GAGGAGCCTC TTTACTGGCC 
AGGCCAGGAC ACAGCTTAGT AGCTGCTATT CGAAGCCAAC ATCAACATGA GTCATTGCCA 
CTGGCATATA ATGACAAGAT TGTGGCATTT CTTCGCCAGC CAAACATTTT TGAAATGCTG 
CAAGAGCGTC AGCCAAGCTT AGCAAGAAAC CACACACTCA GGGAGAAAAT CCATTACATT 
CGGACTGAGG GTAATCACGG GCTTGAGAAG TTGTCCTGTG ATGCGGATCT GGTCATTTTG 
CTGAGTCTCT TTGAAGAAGA GATTATGTCC TACGTCCCCC TGCAGGCTGC CTTCCACCCT 
GGGTATAGCT TCTCTCCTCG CTGTTCACCC TGTTCTTCAC CTCAGAACTC CCCAGGTTTA 
CAGAGAGCCA GCGCAAGAGC CCCTTCCCCC TACCGGAGAG ACTTTGAGGC CAAGCTCCGC 
AATTTCTACA GAAAACTGGA AGCCAAAGGA TTTGGTCAGG GTCCAGGGAA AATTAAGCTC 
ATTATTCGCC GGGATCATTT GTTGGAGGGA ACCTTCAATC AAGTGATGGC TTATTCGCGG 
AAAGAGCTCC AGCGAAACAA GCTCTATGTC ACCTTTGTTG GAGAGGAGGG CCTGGACTAC 
AGTGGCCCCT CGCGGGAATT CTTCTTCCTT CTGTCTCAGG AGCTCTTCAA CCCTTACTAT 
GGACTCTTTG AGTACTCGGC AAATGATACT TACACGGTGC AGATCAGCCC CATGTCCGCA 
TTTGTAGAAA ACCATCTTGA GTGGTTCAGG TTTAGCGGTC GCATCCTGGG CCTGGCTCTG 
ATCCATCAGT ACCTTCTTGA CGCTTTCTTC ACGAGGCCCT TCTACAAGGC ACTCCTGAGA 
CTGCCCTGTG ATTTGAGTGA CCTGGAATAT TTGGATGAGG AATTCCACCA GAGCTTGCAG 
TGGATGAAGG ACAACAACAT CACAGACATC CTAGACCTCA CTTTCACCGT TAATGAAGAG 
GTTTTTGGAC AGGTCACGGA AAGGGAGTTG AAGTCTGGAG GAGCCAACAC ACAGGTGACG 
GAGAAGAACA AGAAGGAGTA CATCGAGCGC ATGGTGAAGT GGCGGGTGGA ACGCGGCGTG 
GTACAGCAGA CCGAGGCACT GGTGCGCGGC TTCTACGAGG TTGTAGACTC GAGGCTGGTG 
TCCGTGTTTG ATGCCAGGGA GCTGGAGCTG GTGATAGCTG GCACCGCGGA AATCGACCTA 
AATGACTGGC GGAATAACAC CGAGTACCGG GGAGGTTACC ACGATGGGCA TCTTGTGATC 
CGCTGGTTCT GGGCTGCAGT GGAGCGCTTC AATAATGAGC AGAGGCTGAG ATTACTGCAG 
TTTGTCACGG GAACATCCAG CGTGCCCTAC GAAGGCTTCG CAGCCCTCCG TGGGAGCAAT 
GGGCTTCGGC GCTTCTGCAT AGAGAAATGG GGGAAAATTA CCTCTCTCCC CAGGGCACAC 
ACATGCTTCA ACCGACTGGA TCTTCCACCG TATCCCTCGT ACTCCATGTT GTATGAAAAG 
CTGTTAACAG CAGTAGAGGA AACCAGCACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028700696.1
CDS	209..4573
Translation	MAALAEPKDDCISKEVKCSGQELQIQKLPGSQIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_028844863.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028844863.1

ORF Insert Sequence:

ATGGCTGCTC TGGCAGAACC CAAAGATGAC TGCATTTCCA AGGAGGTCAA ATGTTCAGGA 
CAAGAACTGC AAATTCAAAA GCTTCCAGGG AGCCAGATTT TTAAAAGCAT CGGCGCTGAT 
GAGACTGTAC AAGGACAAGG AAGTCGGAGG CTGATCAGCT TCTCTCTCTC AGATTTCCAA 
GCCATGGGGT TGAAGAAAGG GATGTTTTTC AACCCAGACC CTTATCTGAA GATTTCCATT 
CAGCCTGGGA AGCACAGCAT CTTCCCCGCC CTCCCTCACC ATGGACAGGA GAGGAGATCC 
AAGATCATAG GCAACACCGT GAACCCCATC TGGCAGGCCG AGCAATTCAG TTTTGTGTCC 
TTGCCCACTG ACGTGCTGGA AATTGAGGTG AAGGACAAGT TTGCCAAGAG CCGTCCCATC 
ATCAAGCGTT TCTTGGGAAA GCTGTCGATG CCCGTTCAAA GACTCCTGGA GAGACACGCC 
ATAGGGGATA GGGTGGTCAG CTACACACTT GGCCGCAGGC TTCCAACAGA TCATGTGAGT 
GGACAGCTGC AATTCCGATT TGAGATCACT TCCTCCATCC ACCCAGCAGA TGATGAGGAG 
ATTTCCCTGA GTACGGAGCC TGAGTCAGCC CCCATTCAGG ACAGCCCCAT GAACAACCTG 
ATGGAAAGCG GCAGTGGGGA ACCTCGATCT GAGGCACCGG AGTCCTCTGA GGGCTGGAAG 
CCAGAGCAGC TGGGTGAGGG CAGTGTCCTG GATGGTCCAG GGAACCAAAG CATGGAGCTT 
TCCAGACCAG CTGAGGAAGC AGCAGTCATC ACGGAGGCAG GAGACCAGGG CATGGTCTCT 
GTGGGACCTG AAGGGGCTGG GGACCTCCTG GCCCAGGTGC AAAAGGACAT CCAGCCTGCC 
CCGAGTGCAG AAGAGCTGGC CGAGCAGCTG GACCTGGGTG AGGAGGCGTC AGCACTGCTG 
CTGGAAGACG GTGAAGCACC AGCCAGCACC GAGGAGGAGC CCTTAGAGGA GGAAGCAACG 
ACACAGAGCC GGGCTGGAAG GGAAGAAGAG AAGGAGCAGG AGGAGGAGGA GGGAGATGTG 
TCTACCCTGG AGCAGGGAGA GGGCAGGCTG CAACTGCGGG CCTCGGTGAA GAGAAAAAGC 
AGGCCCTGCT CCTTGCCTGT GTCTGAGCTG GAGACAGTGA TCGCGTCAGC CTGCGGGGAC 
CCCGAGACCC CGCGGACACA CTACATCCGC ATCCACACCC TGCTGCACAG CATGCCCTCG 
GCCCAGGGCG GCAGCGCGGC AGAGGAGGAG GACGGCGCGG AGGAGGAGTC CACCCTCAAG 
GACTCCTCGG AGAAGGATGG GCTCAGCGAG GTGGACACAG TGGCGGCTGA CCCGTCTGCC 
CTGGAAGAGG ACAGAGAAGA GCCCGAGGGG GCTACTCCAG GCACGGCGCA TCCTGGCCAC 
TCCGGGGGCC ACTTCTCCAG CCTGGCCAAT GGCGCCGCCC AGGATGGCGA CACGCACCCC 
AGCACCGGCA GCGAGAGCGA CTCCAGCCCC AGGCAAGGCG GGGACCACAG TTGCGAGGGC 
TGTGATGCGT CCTGCTGCAG CCCCTCGTGC TACAGCTCCT CGTGCTACAG CACGTCCTGC 
TACAGCAGCT CGTGCTACAG CGCCTCCTGC TACAGCCCCT CCTGCTACAA CGGCAACAGG 
TTCGCCAGCC ACACGCGCTT CTCCTCCGTA GACAGCGCCA AGATCTCCGA GAGCACGGTC 
TTCTCCTCGC AAGATGATGA GGAGGAGGAG AACAGCGCGT TCGAATCTGT GCCCGACTCC 
ATGCAGAGCC CTGAGCTGGA CCCGGAGTCC ACGAACGGCG CTGGGCCGTG GCAAGACGAG 
CTGGCCGCCC CTAGCGGGCA CGAGGAAAGA ACCCCGGAAG GTCTGGAATC CCCCGTGGCA 
GGTCCAAGCA ATCGGAGAGA AGGTGAATGT CCTATACTCC ATAATTCCCA GCCAGTAAGC 
CAGCTTCCTT CCCTGAGGCC TGAACATCAT CACTACCCAA CAATCGATGA GCCTCTTCCA 
CCAAACTGGG AAGCTCGAAT TGACAGCCAC GGGCGGGTCT TTTATGTGGA CCACGTGAAC 
CGCACAACCA CCTGGCAGCG TCCAACGGCA GCAGCCACCC CGGATGGCAT GCGGAGATCG 
GGGTCCATCC AGCAGATGGA GCAACTCAAC AGGCGGTATC AAAACATTCA GCGAACCATT 
GCAACAGAGA GGTCGGAAGA AGATTCTGGC AGCCAAAGCT GCGAGCAAGC CCCAGCAGGA 
GGAGGCGGAG GTGGAGGGAG TGACTCAGAA GCCGAATCTT CCCAGTCCAG CTTAGATCTA 
AGGAGAGAAG GGTCACTTTC TCCGGTTAAC TCACAAAAAA TCACCTTGCT GCTGCAGTCC 
CCAGCGGTCA AGTTCATCAC CAACCCCGAA TTCTTCACTG TGCTGCATGC CAATTATAGT 
GCCTACCGAG TGTTCACCAG TAGCACCTGC TTAAAGCACA TGATTCTGAA AGTCCGACGG 
GATGCTCGCA ATTTTGAACG CTACCAGCAC AACCGGGACT TGGTGAATTT CATCAACATG 
TTCGCAGACA CTCGGCTGGA ACTGCCCCGG GGCTGGGAGA TCAAAACGGA CCAGCAGGGA 
AAGTCTTTTT TCGTGGACCA CAACAGTCGA GCTACCACTT TCATTGACCC CCGAATCCCT 
CTTCAGAACG GTCGTCTTCC CAATCATCTA ACCCACCGAC AGCACCTCCA GAGACTCCGA 
AGTTACAGCG CTGGAGAGGC CTCAGAAGTT TCTAGAAACA GAGGAGCCTC TTTACTGGCC 
AGGCCAGGAC ACAGCTTAGT AGCTGCTATT CGAAGCCAAC ATCAACATGA GTCATTGCCA 
CTGGCATATA ATGACAAGAT TGTGGCATTT CTTCGCCAGC CAAACATTTT TGAAATGCTG 
CAAGAGCGTC AGCCAAGCTT AGCAAGAAAC CACACACTCA GGGAGAAAAT CCATTACATT 
CGGACTGAGG GTAATCACGG GCTTGAGAAG TTGTCCTGTG ATGCGGATCT GGTCATTTTG 
CTGAGTCTCT TTGAAGAAGA GATTATGTCC TACGTCCCCC TGCAGGCTGC CTTCCACCCT 
GGGTATAGCT TCTCTCCTCG CTGTTCACCC TGTTCTTCAC CTCAGAACTC CCCAGGTTTA 
CAGAGAGCCA GCGCAAGAGC CCCTTCCCCC TACCGGAGAG ACTTTGAGGC CAAGCTCCGC 
AATTTCTACA GAAAACTGGA AGCCAAAGGA TTTGGTCAGG GTCCAGGGAA AATTAAGCTC 
ATTATTCGCC GGGATCATTT GTTGGAGGGA ACCTTCAATC AAGTGATGGC TTATTCGCGG 
AAAGAGCTCC AGCGAAACAA GCTCTATGTC ACCTTTGTTG GAGAGGAGGG CCTGGACTAC 
AGTGGCCCCT CGCGGGAATT CTTCTTCCTT CTGTCTCAGG AGCTCTTCAA CCCTTACTAT 
GGACTCTTTG AGTACTCGGC AAATGATACT TACACGGTGC AGATCAGCCC CATGTCCGCA 
TTTGTAGAAA ACCATCTTGA GTGGTTCAGG TTTAGCGGTC GCATCCTGGG CCTGGCTCTG 
ATCCATCAGT ACCTTCTTGA CGCTTTCTTC ACGAGGCCCT TCTACAAGGC ACTCCTGAGA 
CTGCCCTGTG ATTTGAGTGA CCTGGAATAT TTGGATGAGG AATTCCACCA GAGCTTGCAG 
TGGATGAAGG ACAACAACAT CACAGACATC CTAGACCTCA CTTTCACCGT TAATGAAGAG 
GTTTTTGGAC AGGTCACGGA AAGGGAGTTG AAGTCTGGAG GAGCCAACAC ACAGGTGACG 
GAGAAGAACA AGAAGGAGTA CATCGAGCGC ATGGTGAAGT GGCGGGTGGA ACGCGGCGTG 
GTACAGCAGA CCGAGGCACT GGTGCGCGGC TTCTACGAGG TTGTAGACTC GAGGCTGGTG 
TCCGTGTTTG ATGCCAGGGA GCTGGAGCTG GTGATAGCTG GCACCGCGGA AATCGACCTA 
AATGACTGGC GGAATAACAC CGAGTACCGG GGAGGTTACC ACGATGGGCA TCTTGTGATC 
CGCTGGTTCT GGGCTGCAGT GGAGCGCTTC AATAATGAGC AGAGGCTGAG ATTACTGCAG 
TTTGTCACGG GAACATCCAG CGTGCCCTAC GAAGGCTTCG CAGCCCTCCG TGGGAGCAAT 
GGGCTTCGGC GCTTCTGCAT AGAGAAATGG GGGAAAATTA CCTCTCTCCC CAGGGCACAC 
ACATGCTTCA ACCGACTGGA TCTTCCACCG TATCCCTCGT ACTCCATGTT GTATGAAAAG 
CTGTTAACAG CAGTAGAGGA AACCAGCACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015133526.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014989012.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

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CloneID	OMb81458
Clone ID Related Accession (Same CDS sequence)	XM_015133526.2
Accession Version	XM_015133526.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041756.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015133526.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCTCTC 
CGATACAGCT ACAATCCTGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCATGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTACTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCAG CAGCTCCTAT TTTTAAAAGC ATCGGCGCTG ATGAGACTGT ACAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACGGAG 
CCTGAGTCAG CCCCCATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGAT CTGAGGCACC GGAGTCCTCT GAGGGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC TGGATGGTCC AGGGAACCAA AGCATGGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCACGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGACCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCGAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCG TCAGCACTGC TGCTGGAAGA CGGTGAAGCA 
CCAGCCAGCA CCGAGGAGGA GCCCTTAGAG GAGGAAGCAA CGACACAGAG CCGGGCTGGA 
AGGGAAGAAG AGAAGGAGCA GGAGGAGGAG GAGGGAGATG TGTCTACCCT GGAGCAGGGA 
GAGGGCAGGC TGCAACTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCTGAGC TGGAGACAGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC AGTGGCGGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CATCCTGGCC ACTCCGGGGG CCACTTCTCC 
AGCCTGGCCA ATGGCGCCGC CCAGGATGGC GACACGCACC CCAGCACCGG CAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGATGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCCT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TAGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGATGAT 
GAGGAGGAGG AGAACAGCGC GTTCGAATCT GTGCCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGAGGAAA GAACCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGGTGAAT GTCCTATACT CCATAATTCC CAGCCAGTAA GCCAGCTTCC TTCCCTGAGG 
CCTGAACATC ATCACTACCC AACAATCGAT GAGCCTCTTC CACCAAACTG GGAAGCTCGA 
ATTGACAGCC ACGGGCGGGT CTTTTATGTG GACCACGTGA ACCGCACAAC CACCTGGCAG 
CGTCCAACGG CAGCAGCCAC CCCGGATGGC ATGCGGAGAT CGGGGTCCAT CCAGCAGATG 
GAGCAACTCA ACAGGCGGTA TCAAAACATT CAGCGAACCA TTGCAACAGA GAGGTCGGAA 
GAAGATTCTG GCAGCCAAAG CTGCGAGCAA GCCCCAGCAG GAGGAGGCGG AGGTGGAGGG 
AGTGACTCAG AAGCCGAATC TTCCCAGTCC AGCTTAGATC TAAGGAGAGA AGGGTCACTT 
TCTCCGGTTA ACTCACAAAA AATCACCTTG CTGCTGCAGT CCCCAGCGGT CAAGTTCATC 
ACCAACCCCG AATTCTTCAC TGTGCTGCAT GCCAATTATA GTGCCTACCG AGTGTTCACC 
AGTAGCACCT GCTTAAAGCA CATGATTCTG AAAGTCCGAC GGGATGCTCG CAATTTTGAA 
CGCTACCAGC ACAACCGGGA CTTGGTGAAT TTCATCAACA TGTTCGCAGA CACTCGGCTG 
GAACTGCCCC GGGGCTGGGA GATCAAAACG GACCAGCAGG GAAAGTCTTT TTTCGTGGAC 
CACAACAGTC GAGCTACCAC TTTCATTGAC CCCCGAATCC CTCTTCAGAA CGGTCGTCTT 
CCCAATCATC TAACCCACCG ACAGCACCTC CAGAGACTCC GAAGTTACAG CGCTGGAGAG 
GCCTCAGAAG TTTCTAGAAA CAGAGGAGCC TCTTTACTGG CCAGGCCAGG ACACAGCTTA 
GTAGCTGCTA TTCGAAGCCA ACATCAACAT GAGTCATTGC CACTGGCATA TAATGACAAG 
ATTGTGGCAT TTCTTCGCCA GCCAAACATT TTTGAAATGC TGCAAGAGCG TCAGCCAAGC 
TTAGCAAGAA ACCACACACT CAGGGAGAAA ATCCATTACA TTCGGACTGA GGGTAATCAC 
GGGCTTGAGA AGTTGTCCTG TGATGCGGAT CTGGTCATTT TGCTGAGTCT CTTTGAAGAA 
GAGATTATGT CCTACGTCCC CCTGCAGGCT GCCTTCCACC CTGGGTATAG CTTCTCTCCT 
CGCTGTTCAC CCTGTTCTTC ACCTCAGAAC TCCCCAGGTT TACAGAGAGC CAGCGCAAGA 
GCCCCTTCCC CCTACCGGAG AGACTTTGAG GCCAAGCTCC GCAATTTCTA CAGAAAACTG 
GAAGCCAAAG GATTTGGTCA GGGTCCAGGG AAAATTAAGC TCATTATTCG CCGGGATCAT 
TTGTTGGAGG GAACCTTCAA TCAAGTGATG GCTTATTCGC GGAAAGAGCT CCAGCGAAAC 
AAGCTCTATG TCACCTTTGT TGGAGAGGAG GGCCTGGACT ACAGTGGCCC CTCGCGGGAA 
TTCTTCTTCC TTCTGTCTCA GGAGCTCTTC AACCCTTACT ATGGACTCTT TGAGTACTCG 
GCAAATGATA CTTACACGGT GCAGATCAGC CCCATGTCCG CATTTGTAGA AAACCATCTT 
GAGTGGTTCA GGTTTAGCGG TCGCATCCTG GGCCTGGCTC TGATCCATCA GTACCTTCTT 
GACGCTTTCT TCACGAGGCC CTTCTACAAG GCACTCCTGA GACTGCCCTG TGATTTGAGT 
GACCTGGAAT ATTTGGATGA GGAATTCCAC CAGAGCTTGC AGTGGATGAA GGACAACAAC 
ATCACAGACA TCCTAGACCT CACTTTCACC GTTAATGAAG AGGTTTTTGG ACAGGTCACG 
GAAAGGGAGT TGAAGTCTGG AGGAGCCAAC ACACAGGTGA CGGAGAAGAA CAAGAAGGAG 
TACATCGAGC GCATGGTGAA GTGGCGGGTG GAACGCGGCG TGGTACAGCA GACCGAGGCA 
CTGGTGCGCG GCTTCTACGA GGTTGTAGAC TCGAGGCTGG TGTCCGTGTT TGATGCCAGG 
GAGCTGGAGC TGGTGATAGC TGGCACCGCG GAAATCGACC TAAATGACTG GCGGAATAAC 
ACCGAGTACC GGGGAGGTTA CCACGATGGG CATCTTGTGA TCCGCTGGTT CTGGGCTGCA 
GTGGAGCGCT TCAATAATGA GCAGAGGCTG AGATTACTGC AGTTTGTCAC GGGAACATCC 
AGCGTGCCCT ACGAAGGCTT CGCAGCCCTC CGTGGGAGCA ATGGGCTTCG GCGCTTCTGC 
ATAGAGAAAT GGGGGAAAAT TACCTCTCTC CCCAGGGCAC ACACATGCTT CAACCGACTG 
GATCTTCCAC CGTATCCCTC GTACTCCATG TTGTATGAAA AGCTGTTAAC AGCAGTAGAG 
GAAACCAGCA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014989012.1
CDS	342..5168
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015133526.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015133526.2

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCTCTC 
CGATACAGCT ACAATCCTGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCATGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTACTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCAG CAGCTCCTAT TTTTAAAAGC ATCGGCGCTG ATGAGACTGT ACAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACGGAG 
CCTGAGTCAG CCCCCATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGAT CTGAGGCACC GGAGTCCTCT GAGGGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC TGGATGGTCC AGGGAACCAA AGCATGGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCACGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGACCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCGAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCG TCAGCACTGC TGCTGGAAGA CGGTGAAGCA 
CCAGCCAGCA CCGAGGAGGA GCCCTTAGAG GAGGAAGCAA CGACACAGAG CCGGGCTGGA 
AGGGAAGAAG AGAAGGAGCA GGAGGAGGAG GAGGGAGATG TGTCTACCCT GGAGCAGGGA 
GAGGGCAGGC TGCAACTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCTGAGC TGGAGACAGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC AGTGGCGGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CATCCTGGCC ACTCCGGGGG CCACTTCTCC 
AGCCTGGCCA ATGGCGCCGC CCAGGATGGC GACACGCACC CCAGCACCGG CAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGATGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCCT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TAGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGATGAT 
GAGGAGGAGG AGAACAGCGC GTTCGAATCT GTGCCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGAGGAAA GAACCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGGTGAAT GTCCTATACT CCATAATTCC CAGCCAGTAA GCCAGCTTCC TTCCCTGAGG 
CCTGAACATC ATCACTACCC AACAATCGAT GAGCCTCTTC CACCAAACTG GGAAGCTCGA 
ATTGACAGCC ACGGGCGGGT CTTTTATGTG GACCACGTGA ACCGCACAAC CACCTGGCAG 
CGTCCAACGG CAGCAGCCAC CCCGGATGGC ATGCGGAGAT CGGGGTCCAT CCAGCAGATG 
GAGCAACTCA ACAGGCGGTA TCAAAACATT CAGCGAACCA TTGCAACAGA GAGGTCGGAA 
GAAGATTCTG GCAGCCAAAG CTGCGAGCAA GCCCCAGCAG GAGGAGGCGG AGGTGGAGGG 
AGTGACTCAG AAGCCGAATC TTCCCAGTCC AGCTTAGATC TAAGGAGAGA AGGGTCACTT 
TCTCCGGTTA ACTCACAAAA AATCACCTTG CTGCTGCAGT CCCCAGCGGT CAAGTTCATC 
ACCAACCCCG AATTCTTCAC TGTGCTGCAT GCCAATTATA GTGCCTACCG AGTGTTCACC 
AGTAGCACCT GCTTAAAGCA CATGATTCTG AAAGTCCGAC GGGATGCTCG CAATTTTGAA 
CGCTACCAGC ACAACCGGGA CTTGGTGAAT TTCATCAACA TGTTCGCAGA CACTCGGCTG 
GAACTGCCCC GGGGCTGGGA GATCAAAACG GACCAGCAGG GAAAGTCTTT TTTCGTGGAC 
CACAACAGTC GAGCTACCAC TTTCATTGAC CCCCGAATCC CTCTTCAGAA CGGTCGTCTT 
CCCAATCATC TAACCCACCG ACAGCACCTC CAGAGACTCC GAAGTTACAG CGCTGGAGAG 
GCCTCAGAAG TTTCTAGAAA CAGAGGAGCC TCTTTACTGG CCAGGCCAGG ACACAGCTTA 
GTAGCTGCTA TTCGAAGCCA ACATCAACAT GAGTCATTGC CACTGGCATA TAATGACAAG 
ATTGTGGCAT TTCTTCGCCA GCCAAACATT TTTGAAATGC TGCAAGAGCG TCAGCCAAGC 
TTAGCAAGAA ACCACACACT CAGGGAGAAA ATCCATTACA TTCGGACTGA GGGTAATCAC 
GGGCTTGAGA AGTTGTCCTG TGATGCGGAT CTGGTCATTT TGCTGAGTCT CTTTGAAGAA 
GAGATTATGT CCTACGTCCC CCTGCAGGCT GCCTTCCACC CTGGGTATAG CTTCTCTCCT 
CGCTGTTCAC CCTGTTCTTC ACCTCAGAAC TCCCCAGGTT TACAGAGAGC CAGCGCAAGA 
GCCCCTTCCC CCTACCGGAG AGACTTTGAG GCCAAGCTCC GCAATTTCTA CAGAAAACTG 
GAAGCCAAAG GATTTGGTCA GGGTCCAGGG AAAATTAAGC TCATTATTCG CCGGGATCAT 
TTGTTGGAGG GAACCTTCAA TCAAGTGATG GCTTATTCGC GGAAAGAGCT CCAGCGAAAC 
AAGCTCTATG TCACCTTTGT TGGAGAGGAG GGCCTGGACT ACAGTGGCCC CTCGCGGGAA 
TTCTTCTTCC TTCTGTCTCA GGAGCTCTTC AACCCTTACT ATGGACTCTT TGAGTACTCG 
GCAAATGATA CTTACACGGT GCAGATCAGC CCCATGTCCG CATTTGTAGA AAACCATCTT 
GAGTGGTTCA GGTTTAGCGG TCGCATCCTG GGCCTGGCTC TGATCCATCA GTACCTTCTT 
GACGCTTTCT TCACGAGGCC CTTCTACAAG GCACTCCTGA GACTGCCCTG TGATTTGAGT 
GACCTGGAAT ATTTGGATGA GGAATTCCAC CAGAGCTTGC AGTGGATGAA GGACAACAAC 
ATCACAGACA TCCTAGACCT CACTTTCACC GTTAATGAAG AGGTTTTTGG ACAGGTCACG 
GAAAGGGAGT TGAAGTCTGG AGGAGCCAAC ACACAGGTGA CGGAGAAGAA CAAGAAGGAG 
TACATCGAGC GCATGGTGAA GTGGCGGGTG GAACGCGGCG TGGTACAGCA GACCGAGGCA 
CTGGTGCGCG GCTTCTACGA GGTTGTAGAC TCGAGGCTGG TGTCCGTGTT TGATGCCAGG 
GAGCTGGAGC TGGTGATAGC TGGCACCGCG GAAATCGACC TAAATGACTG GCGGAATAAC 
ACCGAGTACC GGGGAGGTTA CCACGATGGG CATCTTGTGA TCCGCTGGTT CTGGGCTGCA 
GTGGAGCGCT TCAATAATGA GCAGAGGCTG AGATTACTGC AGTTTGTCAC GGGAACATCC 
AGCGTGCCCT ACGAAGGCTT CGCAGCCCTC CGTGGGAGCA ATGGGCTTCG GCGCTTCTGC 
ATAGAGAAAT GGGGGAAAAT TACCTCTCTC CCCAGGGCAC ACACATGCTT CAACCGACTG 
GATCTTCCAC CGTATCCCTC GTACTCCATG TTGTATGAAA AGCTGTTAAC AGCAGTAGAG 
GAAACCAGCA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015133527.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014989013.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMb81459
Clone ID Related Accession (Same CDS sequence)	XM_015133527.2
Accession Version	XM_015133527.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041756.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015133527.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCTCTC 
CGATACAGCT ACAATCCTGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCATGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTACTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCAG CAGCTCCTAT TTTTAAAAGC ATCGGCGCTG ATGAGACTGT ACAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCAACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCGGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCGGTTAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAAT TCTTCACTGT GCTGCATGCC AATTATAGTG CCTACCGAGT GTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CCCACCGACA GCACCTCCAG AGACTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCTCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC 
CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA AGTGATGGCT TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTATGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGCTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCC TAGACCTCAC TTTCACCGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA CGCGGCGTGG TACAGCAGAC CGAGGCACTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCAGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC CTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014989013.1
CDS	342..5165
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015133527.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015133527.2

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCTCTC 
CGATACAGCT ACAATCCTGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCATGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTACTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCAG CAGCTCCTAT TTTTAAAAGC ATCGGCGCTG ATGAGACTGT ACAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCAACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCGGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCGGTTAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAAT TCTTCACTGT GCTGCATGCC AATTATAGTG CCTACCGAGT GTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CCCACCGACA GCACCTCCAG AGACTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCTCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC 
CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA AGTGATGGCT TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTATGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGCTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCC TAGACCTCAC TTTCACCGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA CGCGGCGTGG TACAGCAGAC CGAGGCACTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCAGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC CTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015133529.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014989015.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

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CloneID	OMb81460
Clone ID Related Accession (Same CDS sequence)	XM_015133529.2 , XM_015133530.2 , XM_028844861.1
Accession Version	XM_015133529.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041756.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015133529.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCAACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCGGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCGGTTAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAAT TCTTCACTGT GCTGCATGCC AATTATAGTG CCTACCGAGT GTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CCCACCGACA GCACCTCCAG AGACTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCTCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC 
CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA AGTGATGGCT TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTATGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGCTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCC TAGACCTCAC TTTCACCGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA CGCGGCGTGG TACAGCAGAC CGAGGCACTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCAGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC CTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014989015.1
CDS	679..5502
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015133529.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015133529.2

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCAACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCGGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCGGTTAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAAT TCTTCACTGT GCTGCATGCC AATTATAGTG CCTACCGAGT GTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CCCACCGACA GCACCTCCAG AGACTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCTCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC 
CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA AGTGATGGCT TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTATGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGCTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCC TAGACCTCAC TTTCACCGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA CGCGGCGTGG TACAGCAGAC CGAGGCACTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCAGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC CTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015133530.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014989016.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

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CloneID	OMb81460
Clone ID Related Accession (Same CDS sequence)	XM_015133529.2 , XM_015133530.2 , XM_028844861.1
Accession Version	XM_015133530.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041756.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015133530.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCAACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCGGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCGGTTAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAAT TCTTCACTGT GCTGCATGCC AATTATAGTG CCTACCGAGT GTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CCCACCGACA GCACCTCCAG AGACTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCTCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC 
CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA AGTGATGGCT TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTATGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGCTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCC TAGACCTCAC TTTCACCGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA CGCGGCGTGG TACAGCAGAC CGAGGCACTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCAGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC CTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014989016.1
CDS	403..5226
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015133530.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015133530.2

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCAACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCGGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCGGTTAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAAT TCTTCACTGT GCTGCATGCC AATTATAGTG CCTACCGAGT GTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CCCACCGACA GCACCTCCAG AGACTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCTCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC 
CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA AGTGATGGCT TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTATGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGCTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCC TAGACCTCAC TTTCACCGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA CGCGGCGTGG TACAGCAGAC CGAGGCACTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCAGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC CTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_028844861.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028700694.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

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CloneID	OMb81460
Clone ID Related Accession (Same CDS sequence)	XM_015133529.2 , XM_015133530.2 , XM_028844861.1
Accession Version	XM_028844861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041756.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCAACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCGGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCGGTTAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAAT TCTTCACTGT GCTGCATGCC AATTATAGTG CCTACCGAGT GTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CCCACCGACA GCACCTCCAG AGACTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCTCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC 
CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA AGTGATGGCT TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTATGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGCTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCC TAGACCTCAC TTTCACCGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA CGCGGCGTGG TACAGCAGAC CGAGGCACTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCAGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC CTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028700694.1
CDS	553..5376
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_028844861.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028844861.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCAACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCGGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCGGTTAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAAT TCTTCACTGT GCTGCATGCC AATTATAGTG CCTACCGAGT GTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CCCACCGACA GCACCTCCAG AGACTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCTCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC 
CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA AGTGATGGCT TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTATGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGCTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCC TAGACCTCAC TTTCACCGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA CGCGGCGTGG TACAGCAGAC CGAGGCACTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCAGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC CTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_028844862.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028700695.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMb81461
Clone ID Related Accession (Same CDS sequence)	XM_028844862.1
Accession Version	XM_028844862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041756.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC GGAGCCTGAG 
TCAGCCCCCA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGATCTGAGG CACCGGAGTC CTCTGAGGGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCTGGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAC 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCGA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCACCAGCC 
AGCACCGAGG AGGAGCCCTT AGAGGAGGAA GCAACGACAC AGAGCCGGGC TGGAAGGGAA 
GAAGAGAAGG AGCAGGAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAAC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCT 
GAGCTGGAGA CAGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCGGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACAGTGGC GGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCATCCT GGCCACTCCG GGGGCCACTT CTCCAGCCTG 
GCCAATGGCG CCGCCCAGGA TGGCGACACG CACCCCAGCA CCGGCAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ATGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCCTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTAGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA TGATGAGGAG 
GAGGAGAACA GCGCGTTCGA ATCTGTGCCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGAG 
GAAAGAACCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCA 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC GGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCG 
GTTAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAATTCT TCACTGTGCT GCATGCCAAT TATAGTGCCT ACCGAGTGTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACCC ACCGACAGCA CCTCCAGAGA CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCTCGCTGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGCGC AAGAGCCCCT 
TCCCCCTACC GGAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC AGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAAGT GATGGCTTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TATGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAATTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGCCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGC TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCCTAG ACCTCACTTT CACCGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACGGAGA AGAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAACGC GGCGTGGTAC AGCAGACCGA GGCACTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACCGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCAGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACCTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028700695.1
CDS	601..5421
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_028844862.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028844862.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC GGAGCCTGAG 
TCAGCCCCCA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGATCTGAGG CACCGGAGTC CTCTGAGGGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCTGGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAC 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCGA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCACCAGCC 
AGCACCGAGG AGGAGCCCTT AGAGGAGGAA GCAACGACAC AGAGCCGGGC TGGAAGGGAA 
GAAGAGAAGG AGCAGGAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAAC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCT 
GAGCTGGAGA CAGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCGGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACAGTGGC GGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCATCCT GGCCACTCCG GGGGCCACTT CTCCAGCCTG 
GCCAATGGCG CCGCCCAGGA TGGCGACACG CACCCCAGCA CCGGCAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ATGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCCTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTAGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA TGATGAGGAG 
GAGGAGAACA GCGCGTTCGA ATCTGTGCCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGAG 
GAAAGAACCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCA 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC GGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCG 
GTTAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAATTCT TCACTGTGCT GCATGCCAAT TATAGTGCCT ACCGAGTGTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACCC ACCGACAGCA CCTCCAGAGA CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCTCGCTGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGCGC AAGAGCCCCT 
TCCCCCTACC GGAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC AGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAAGT GATGGCTTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TATGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAATTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGCCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGC TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCCTAG ACCTCACTTT CACCGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACGGAGA AGAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAACGC GGCGTGGTAC AGCAGACCGA GGCACTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACCGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCAGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACCTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015133531.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014989017.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMb81462
Clone ID Related Accession (Same CDS sequence)	XM_015133531.2
Accession Version	XM_015133531.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041756.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015133531.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCTCTC 
CGATACAGCT ACAATCCTGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCATGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTACTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCAG CAGCTCCTAT TTTTAAAAGC ATCGGCGCTG ATGAGACTGT ACAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACGGAG 
CCTGAGTCAG CCCCCATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGAT CTGAGGCACC GGAGTCCTCT GAGGGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC TGGATGGTCC AGGGAACCAA AGCATGGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCACGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGACCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCGAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCG TCAGCACTGC TGCTGGAAGA CGGTGAAGCA 
CCAGCCAGCA CCGAGGAGGA GCCCTTAGAG GAGGAAGCAA CGACACAGAG CCGGGCTGGA 
AGGGAAGAAG AGAAGGAGCA GGAGGAGGAG GAGGGAGATG TGTCTACCCT GGAGCAGGGA 
GAGGGCAGGC TGCAACTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCTGAGC TGGAGACAGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC AGTGGCGGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CATCCTGGCC ACTCCGGGGG CCACTTCTCC 
AGCCTGGCCA ATGGCGCCGC CCAGGATGGC GACACGCACC CCAGCACCGG CAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGATGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCCT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TAGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGATGAT 
GAGGAGGAGG AGAACAGCGC GTTCGAATCT GTGCCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGAGGAAA GAACCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGACTGGG AAGCTCGAAT TGACAGCCAC GGGCGGGTCT TTTATGTGGA CCACGTGAAC 
CGCACAACCA CCTGGCAGCG TCCAACGGCA GCAGCCACCC CGGATGGCAT GCGGAGATCG 
GGGTCCATCC AGCAGATGGA GCAACTCAAC AGGCGGTATC AAAACATTCA GCGAACCATT 
GCAACAGAGA GGTCGGAAGA AGATTCTGGC AGCCAAAGCT GCGAGCAAGC CCCAGCAGGA 
GGAGGCGGAG GTGGAGGGAG TGACTCAGAA GCCGAATCTT CCCAGTCCAG CTTAGATCTA 
AGGAGAGAAG GGTCACTTTC TCCGGTTAAC TCACAAAAAA TCACCTTGCT GCTGCAGTCC 
CCAGCGGTCA AGTTCATCAC CAACCCCGAA TTCTTCACTG TGCTGCATGC CAATTATAGT 
GCCTACCGAG TGTTCACCAG TAGCACCTGC TTAAAGCACA TGATTCTGAA AGTCCGACGG 
GATGCTCGCA ATTTTGAACG CTACCAGCAC AACCGGGACT TGGTGAATTT CATCAACATG 
TTCGCAGACA CTCGGCTGGA ACTGCCCCGG GGCTGGGAGA TCAAAACGGA CCAGCAGGGA 
AAGTCTTTTT TCGTGGACCA CAACAGTCGA GCTACCACTT TCATTGACCC CCGAATCCCT 
CTTCAGAACG GTCGTCTTCC CAATCATCTA ACCCACCGAC AGCACCTCCA GAGACTCCGA 
AGTTACAGCG CTGGAGAGGC CTCAGAAGTT TCTAGAAACA GAGGAGCCTC TTTACTGGCC 
AGGCCAGGAC ACAGCTTAGT AGCTGCTATT CGAAGCCAAC ATCAACATGA GTCATTGCCA 
CTGGCATATA ATGACAAGAT TGTGGCATTT CTTCGCCAGC CAAACATTTT TGAAATGCTG 
CAAGAGCGTC AGCCAAGCTT AGCAAGAAAC CACACACTCA GGGAGAAAAT CCATTACATT 
CGGACTGAGG GTAATCACGG GCTTGAGAAG TTGTCCTGTG ATGCGGATCT GGTCATTTTG 
CTGAGTCTCT TTGAAGAAGA GATTATGTCC TACGTCCCCC TGCAGGCTGC CTTCCACCCT 
GGGTATAGCT TCTCTCCTCG CTGTTCACCC TGTTCTTCAC CTCAGAACTC CCCAGGTTTA 
CAGAGAGCCA GCGCAAGAGC CCCTTCCCCC TACCGGAGAG ACTTTGAGGC CAAGCTCCGC 
AATTTCTACA GAAAACTGGA AGCCAAAGGA TTTGGTCAGG GTCCAGGGAA AATTAAGCTC 
ATTATTCGCC GGGATCATTT GTTGGAGGGA ACCTTCAATC AAGTGATGGC TTATTCGCGG 
AAAGAGCTCC AGCGAAACAA GCTCTATGTC ACCTTTGTTG GAGAGGAGGG CCTGGACTAC 
AGTGGCCCCT CGCGGGAATT CTTCTTCCTT CTGTCTCAGG AGCTCTTCAA CCCTTACTAT 
GGACTCTTTG AGTACTCGGC AAATGATACT TACACGGTGC AGATCAGCCC CATGTCCGCA 
TTTGTAGAAA ACCATCTTGA GTGGTTCAGG TTTAGCGGTC GCATCCTGGG CCTGGCTCTG 
ATCCATCAGT ACCTTCTTGA CGCTTTCTTC ACGAGGCCCT TCTACAAGGC ACTCCTGAGA 
CTGCCCTGTG ATTTGAGTGA CCTGGAATAT TTGGATGAGG AATTCCACCA GAGCTTGCAG 
TGGATGAAGG ACAACAACAT CACAGACATC CTAGACCTCA CTTTCACCGT TAATGAAGAG 
GTTTTTGGAC AGGTCACGGA AAGGGAGTTG AAGTCTGGAG GAGCCAACAC ACAGGTGACG 
GAGAAGAACA AGAAGGAGTA CATCGAGCGC ATGGTGAAGT GGCGGGTGGA ACGCGGCGTG 
GTACAGCAGA CCGAGGCACT GGTGCGCGGC TTCTACGAGG TTGTAGACTC GAGGCTGGTG 
TCCGTGTTTG ATGCCAGGGA GCTGGAGCTG GTGATAGCTG GCACCGCGGA AATCGACCTA 
AATGACTGGC GGAATAACAC CGAGTACCGG GGAGGTTACC ACGATGGGCA TCTTGTGATC 
CGCTGGTTCT GGGCTGCAGT GGAGCGCTTC AATAATGAGC AGAGGCTGAG ATTACTGCAG 
TTTGTCACGG GAACATCCAG CGTGCCCTAC GAAGGCTTCG CAGCCCTCCG TGGGAGCAAT 
GGGCTTCGGC GCTTCTGCAT AGAGAAATGG GGGAAAATTA CCTCTCTCCC CAGGGCACAC 
ACATGCTTCA ACCGACTGGA TCTTCCACCG TATCCCTCGT ACTCCATGTT GTATGAAAAG 
CTGTTAACAG CAGTAGAGGA AACCAGCACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014989017.1
CDS	342..5066
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015133531.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015133531.2

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCTCTC 
CGATACAGCT ACAATCCTGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCATGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTACTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCAG CAGCTCCTAT TTTTAAAAGC ATCGGCGCTG ATGAGACTGT ACAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACGGAG 
CCTGAGTCAG CCCCCATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGAT CTGAGGCACC GGAGTCCTCT GAGGGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC TGGATGGTCC AGGGAACCAA AGCATGGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCACGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGACCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCGAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCG TCAGCACTGC TGCTGGAAGA CGGTGAAGCA 
CCAGCCAGCA CCGAGGAGGA GCCCTTAGAG GAGGAAGCAA CGACACAGAG CCGGGCTGGA 
AGGGAAGAAG AGAAGGAGCA GGAGGAGGAG GAGGGAGATG TGTCTACCCT GGAGCAGGGA 
GAGGGCAGGC TGCAACTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCTGAGC TGGAGACAGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC AGTGGCGGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CATCCTGGCC ACTCCGGGGG CCACTTCTCC 
AGCCTGGCCA ATGGCGCCGC CCAGGATGGC GACACGCACC CCAGCACCGG CAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGATGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCCT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TAGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGATGAT 
GAGGAGGAGG AGAACAGCGC GTTCGAATCT GTGCCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGAGGAAA GAACCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGACTGGG AAGCTCGAAT TGACAGCCAC GGGCGGGTCT TTTATGTGGA CCACGTGAAC 
CGCACAACCA CCTGGCAGCG TCCAACGGCA GCAGCCACCC CGGATGGCAT GCGGAGATCG 
GGGTCCATCC AGCAGATGGA GCAACTCAAC AGGCGGTATC AAAACATTCA GCGAACCATT 
GCAACAGAGA GGTCGGAAGA AGATTCTGGC AGCCAAAGCT GCGAGCAAGC CCCAGCAGGA 
GGAGGCGGAG GTGGAGGGAG TGACTCAGAA GCCGAATCTT CCCAGTCCAG CTTAGATCTA 
AGGAGAGAAG GGTCACTTTC TCCGGTTAAC TCACAAAAAA TCACCTTGCT GCTGCAGTCC 
CCAGCGGTCA AGTTCATCAC CAACCCCGAA TTCTTCACTG TGCTGCATGC CAATTATAGT 
GCCTACCGAG TGTTCACCAG TAGCACCTGC TTAAAGCACA TGATTCTGAA AGTCCGACGG 
GATGCTCGCA ATTTTGAACG CTACCAGCAC AACCGGGACT TGGTGAATTT CATCAACATG 
TTCGCAGACA CTCGGCTGGA ACTGCCCCGG GGCTGGGAGA TCAAAACGGA CCAGCAGGGA 
AAGTCTTTTT TCGTGGACCA CAACAGTCGA GCTACCACTT TCATTGACCC CCGAATCCCT 
CTTCAGAACG GTCGTCTTCC CAATCATCTA ACCCACCGAC AGCACCTCCA GAGACTCCGA 
AGTTACAGCG CTGGAGAGGC CTCAGAAGTT TCTAGAAACA GAGGAGCCTC TTTACTGGCC 
AGGCCAGGAC ACAGCTTAGT AGCTGCTATT CGAAGCCAAC ATCAACATGA GTCATTGCCA 
CTGGCATATA ATGACAAGAT TGTGGCATTT CTTCGCCAGC CAAACATTTT TGAAATGCTG 
CAAGAGCGTC AGCCAAGCTT AGCAAGAAAC CACACACTCA GGGAGAAAAT CCATTACATT 
CGGACTGAGG GTAATCACGG GCTTGAGAAG TTGTCCTGTG ATGCGGATCT GGTCATTTTG 
CTGAGTCTCT TTGAAGAAGA GATTATGTCC TACGTCCCCC TGCAGGCTGC CTTCCACCCT 
GGGTATAGCT TCTCTCCTCG CTGTTCACCC TGTTCTTCAC CTCAGAACTC CCCAGGTTTA 
CAGAGAGCCA GCGCAAGAGC CCCTTCCCCC TACCGGAGAG ACTTTGAGGC CAAGCTCCGC 
AATTTCTACA GAAAACTGGA AGCCAAAGGA TTTGGTCAGG GTCCAGGGAA AATTAAGCTC 
ATTATTCGCC GGGATCATTT GTTGGAGGGA ACCTTCAATC AAGTGATGGC TTATTCGCGG 
AAAGAGCTCC AGCGAAACAA GCTCTATGTC ACCTTTGTTG GAGAGGAGGG CCTGGACTAC 
AGTGGCCCCT CGCGGGAATT CTTCTTCCTT CTGTCTCAGG AGCTCTTCAA CCCTTACTAT 
GGACTCTTTG AGTACTCGGC AAATGATACT TACACGGTGC AGATCAGCCC CATGTCCGCA 
TTTGTAGAAA ACCATCTTGA GTGGTTCAGG TTTAGCGGTC GCATCCTGGG CCTGGCTCTG 
ATCCATCAGT ACCTTCTTGA CGCTTTCTTC ACGAGGCCCT TCTACAAGGC ACTCCTGAGA 
CTGCCCTGTG ATTTGAGTGA CCTGGAATAT TTGGATGAGG AATTCCACCA GAGCTTGCAG 
TGGATGAAGG ACAACAACAT CACAGACATC CTAGACCTCA CTTTCACCGT TAATGAAGAG 
GTTTTTGGAC AGGTCACGGA AAGGGAGTTG AAGTCTGGAG GAGCCAACAC ACAGGTGACG 
GAGAAGAACA AGAAGGAGTA CATCGAGCGC ATGGTGAAGT GGCGGGTGGA ACGCGGCGTG 
GTACAGCAGA CCGAGGCACT GGTGCGCGGC TTCTACGAGG TTGTAGACTC GAGGCTGGTG 
TCCGTGTTTG ATGCCAGGGA GCTGGAGCTG GTGATAGCTG GCACCGCGGA AATCGACCTA 
AATGACTGGC GGAATAACAC CGAGTACCGG GGAGGTTACC ACGATGGGCA TCTTGTGATC 
CGCTGGTTCT GGGCTGCAGT GGAGCGCTTC AATAATGAGC AGAGGCTGAG ATTACTGCAG 
TTTGTCACGG GAACATCCAG CGTGCCCTAC GAAGGCTTCG CAGCCCTCCG TGGGAGCAAT 
GGGCTTCGGC GCTTCTGCAT AGAGAAATGG GGGAAAATTA CCTCTCTCCC CAGGGCACAC 
ACATGCTTCA ACCGACTGGA TCTTCCACCG TATCCCTCGT ACTCCATGTT GTATGAAAAG 
CTGTTAACAG CAGTAGAGGA AACCAGCACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015133532.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014989018.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.

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CloneID	OMb81463
Clone ID Related Accession (Same CDS sequence)	XM_015133532.2
Accession Version	XM_015133532.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041756.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015133532.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCTCTC 
CGATACAGCT ACAATCCTGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCATGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTACTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCAG CAGCTCCTAT TTTTAAAAGC ATCGGCGCTG ATGAGACTGT ACAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GACTGGGAAG CTCGAATTGA CAGCCACGGG CGGGTCTTTT ATGTGGACCA CGTGAACCGC 
ACAACCACCT GGCAGCGTCC AACGGCAGCA GCCACCCCGG ATGGCATGCG GAGATCGGGG 
TCCATCCAGC AGATGGAGCA ACTCAACAGG CGGTATCAAA ACATTCAGCG AACCATTGCA 
ACAGAGAGGT CGGAAGAAGA TTCTGGCAGC CAAAGCTGCG AGCAAGCCCC AGCAGGAGGA 
GGCGGAGGTG GAGGGAGTGA CTCAGAAGCC GAATCTTCCC AGTCCAGCTT AGATCTAAGG 
AGAGAAGGGT CACTTTCTCC GGTTAACTCA CAAAAAATCA CCTTGCTGCT GCAGTCCCCA 
GCGGTCAAGT TCATCACCAA CCCCGAATTC TTCACTGTGC TGCATGCCAA TTATAGTGCC 
TACCGAGTGT TCACCAGTAG CACCTGCTTA AAGCACATGA TTCTGAAAGT CCGACGGGAT 
GCTCGCAATT TTGAACGCTA CCAGCACAAC CGGGACTTGG TGAATTTCAT CAACATGTTC 
GCAGACACTC GGCTGGAACT GCCCCGGGGC TGGGAGATCA AAACGGACCA GCAGGGAAAG 
TCTTTTTTCG TGGACCACAA CAGTCGAGCT ACCACTTTCA TTGACCCCCG AATCCCTCTT 
CAGAACGGTC GTCTTCCCAA TCATCTAACC CACCGACAGC ACCTCCAGAG ACTCCGAAGT 
TACAGCGCTG GAGAGGCCTC AGAAGTTTCT AGAAACAGAG GAGCCTCTTT ACTGGCCAGG 
CCAGGACACA GCTTAGTAGC TGCTATTCGA AGCCAACATC AACATGAGTC ATTGCCACTG 
GCATATAATG ACAAGATTGT GGCATTTCTT CGCCAGCCAA ACATTTTTGA AATGCTGCAA 
GAGCGTCAGC CAAGCTTAGC AAGAAACCAC ACACTCAGGG AGAAAATCCA TTACATTCGG 
ACTGAGGGTA ATCACGGGCT TGAGAAGTTG TCCTGTGATG CGGATCTGGT CATTTTGCTG 
AGTCTCTTTG AAGAAGAGAT TATGTCCTAC GTCCCCCTGC AGGCTGCCTT CCACCCTGGG 
TATAGCTTCT CTCCTCGCTG TTCACCCTGT TCTTCACCTC AGAACTCCCC AGGTTTACAG 
AGAGCCAGCG CAAGAGCCCC TTCCCCCTAC CGGAGAGACT TTGAGGCCAA GCTCCGCAAT 
TTCTACAGAA AACTGGAAGC CAAAGGATTT GGTCAGGGTC CAGGGAAAAT TAAGCTCATT 
ATTCGCCGGG ATCATTTGTT GGAGGGAACC TTCAATCAAG TGATGGCTTA TTCGCGGAAA 
GAGCTCCAGC GAAACAAGCT CTATGTCACC TTTGTTGGAG AGGAGGGCCT GGACTACAGT 
GGCCCCTCGC GGGAATTCTT CTTCCTTCTG TCTCAGGAGC TCTTCAACCC TTACTATGGA 
CTCTTTGAGT ACTCGGCAAA TGATACTTAC ACGGTGCAGA TCAGCCCCAT GTCCGCATTT 
GTAGAAAACC ATCTTGAGTG GTTCAGGTTT AGCGGTCGCA TCCTGGGCCT GGCTCTGATC 
CATCAGTACC TTCTTGACGC TTTCTTCACG AGGCCCTTCT ACAAGGCACT CCTGAGACTG 
CCCTGTGATT TGAGTGACCT GGAATATTTG GATGAGGAAT TCCACCAGAG CTTGCAGTGG 
ATGAAGGACA ACAACATCAC AGACATCCTA GACCTCACTT TCACCGTTAA TGAAGAGGTT 
TTTGGACAGG TCACGGAAAG GGAGTTGAAG TCTGGAGGAG CCAACACACA GGTGACGGAG 
AAGAACAAGA AGGAGTACAT CGAGCGCATG GTGAAGTGGC GGGTGGAACG CGGCGTGGTA 
CAGCAGACCG AGGCACTGGT GCGCGGCTTC TACGAGGTTG TAGACTCGAG GCTGGTGTCC 
GTGTTTGATG CCAGGGAGCT GGAGCTGGTG ATAGCTGGCA CCGCGGAAAT CGACCTAAAT 
GACTGGCGGA ATAACACCGA GTACCGGGGA GGTTACCACG ATGGGCATCT TGTGATCCGC 
TGGTTCTGGG CTGCAGTGGA GCGCTTCAAT AATGAGCAGA GGCTGAGATT ACTGCAGTTT 
GTCACGGGAA CATCCAGCGT GCCCTACGAA GGCTTCGCAG CCCTCCGTGG GAGCAATGGG 
CTTCGGCGCT TCTGCATAGA GAAATGGGGG AAAATTACCT CTCTCCCCAG GGCACACACA 
TGCTTCAACC GACTGGATCT TCCACCGTAT CCCTCGTACT CCATGTTGTA TGAAAAGCTG 
TTAACAGCAG TAGAGGAAAC CAGCACCTTT GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014989018.1
CDS	342..5063
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015133532.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015133532.2

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCTCTC 
CGATACAGCT ACAATCCTGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCATGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTACTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCAG CAGCTCCTAT TTTTAAAAGC ATCGGCGCTG ATGAGACTGT ACAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GACTGGGAAG CTCGAATTGA CAGCCACGGG CGGGTCTTTT ATGTGGACCA CGTGAACCGC 
ACAACCACCT GGCAGCGTCC AACGGCAGCA GCCACCCCGG ATGGCATGCG GAGATCGGGG 
TCCATCCAGC AGATGGAGCA ACTCAACAGG CGGTATCAAA ACATTCAGCG AACCATTGCA 
ACAGAGAGGT CGGAAGAAGA TTCTGGCAGC CAAAGCTGCG AGCAAGCCCC AGCAGGAGGA 
GGCGGAGGTG GAGGGAGTGA CTCAGAAGCC GAATCTTCCC AGTCCAGCTT AGATCTAAGG 
AGAGAAGGGT CACTTTCTCC GGTTAACTCA CAAAAAATCA CCTTGCTGCT GCAGTCCCCA 
GCGGTCAAGT TCATCACCAA CCCCGAATTC TTCACTGTGC TGCATGCCAA TTATAGTGCC 
TACCGAGTGT TCACCAGTAG CACCTGCTTA AAGCACATGA TTCTGAAAGT CCGACGGGAT 
GCTCGCAATT TTGAACGCTA CCAGCACAAC CGGGACTTGG TGAATTTCAT CAACATGTTC 
GCAGACACTC GGCTGGAACT GCCCCGGGGC TGGGAGATCA AAACGGACCA GCAGGGAAAG 
TCTTTTTTCG TGGACCACAA CAGTCGAGCT ACCACTTTCA TTGACCCCCG AATCCCTCTT 
CAGAACGGTC GTCTTCCCAA TCATCTAACC CACCGACAGC ACCTCCAGAG ACTCCGAAGT 
TACAGCGCTG GAGAGGCCTC AGAAGTTTCT AGAAACAGAG GAGCCTCTTT ACTGGCCAGG 
CCAGGACACA GCTTAGTAGC TGCTATTCGA AGCCAACATC AACATGAGTC ATTGCCACTG 
GCATATAATG ACAAGATTGT GGCATTTCTT CGCCAGCCAA ACATTTTTGA AATGCTGCAA 
GAGCGTCAGC CAAGCTTAGC AAGAAACCAC ACACTCAGGG AGAAAATCCA TTACATTCGG 
ACTGAGGGTA ATCACGGGCT TGAGAAGTTG TCCTGTGATG CGGATCTGGT CATTTTGCTG 
AGTCTCTTTG AAGAAGAGAT TATGTCCTAC GTCCCCCTGC AGGCTGCCTT CCACCCTGGG 
TATAGCTTCT CTCCTCGCTG TTCACCCTGT TCTTCACCTC AGAACTCCCC AGGTTTACAG 
AGAGCCAGCG CAAGAGCCCC TTCCCCCTAC CGGAGAGACT TTGAGGCCAA GCTCCGCAAT 
TTCTACAGAA AACTGGAAGC CAAAGGATTT GGTCAGGGTC CAGGGAAAAT TAAGCTCATT 
ATTCGCCGGG ATCATTTGTT GGAGGGAACC TTCAATCAAG TGATGGCTTA TTCGCGGAAA 
GAGCTCCAGC GAAACAAGCT CTATGTCACC TTTGTTGGAG AGGAGGGCCT GGACTACAGT 
GGCCCCTCGC GGGAATTCTT CTTCCTTCTG TCTCAGGAGC TCTTCAACCC TTACTATGGA 
CTCTTTGAGT ACTCGGCAAA TGATACTTAC ACGGTGCAGA TCAGCCCCAT GTCCGCATTT 
GTAGAAAACC ATCTTGAGTG GTTCAGGTTT AGCGGTCGCA TCCTGGGCCT GGCTCTGATC 
CATCAGTACC TTCTTGACGC TTTCTTCACG AGGCCCTTCT ACAAGGCACT CCTGAGACTG 
CCCTGTGATT TGAGTGACCT GGAATATTTG GATGAGGAAT TCCACCAGAG CTTGCAGTGG 
ATGAAGGACA ACAACATCAC AGACATCCTA GACCTCACTT TCACCGTTAA TGAAGAGGTT 
TTTGGACAGG TCACGGAAAG GGAGTTGAAG TCTGGAGGAG CCAACACACA GGTGACGGAG 
AAGAACAAGA AGGAGTACAT CGAGCGCATG GTGAAGTGGC GGGTGGAACG CGGCGTGGTA 
CAGCAGACCG AGGCACTGGT GCGCGGCTTC TACGAGGTTG TAGACTCGAG GCTGGTGTCC 
GTGTTTGATG CCAGGGAGCT GGAGCTGGTG ATAGCTGGCA CCGCGGAAAT CGACCTAAAT 
GACTGGCGGA ATAACACCGA GTACCGGGGA GGTTACCACG ATGGGCATCT TGTGATCCGC 
TGGTTCTGGG CTGCAGTGGA GCGCTTCAAT AATGAGCAGA GGCTGAGATT ACTGCAGTTT 
GTCACGGGAA CATCCAGCGT GCCCTACGAA GGCTTCGCAG CCCTCCGTGG GAGCAATGGG 
CTTCGGCGCT TCTGCATAGA GAAATGGGGG AAAATTACCT CTCTCCCCAG GGCACACACA 
TGCTTCAACC GACTGGATCT TCCACCGTAT CCCTCGTACT CCATGTTGTA TGAAAAGCTG 
TTAACAGCAG TAGAGGAAAC CAGCACCTTT GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015133533.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014989019.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA.

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CloneID	OMb81464
Clone ID Related Accession (Same CDS sequence)	XM_015133533.2
Accession Version	XM_015133533.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041756.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015133533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC GGAGCCTGAG 
TCAGCCCCCA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGATCTGAGG CACCGGAGTC CTCTGAGGGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCTGGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAC 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCGA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCACCAGCC 
AGCACCGAGG AGGAGCCCTT AGAGGAGGAA GCAACGACAC AGAGCCGGGC TGGAAGGGAA 
GAAGAGAAGG AGCAGGAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAAC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCT 
GAGCTGGAGA CAGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCGGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACAGTGGC GGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCATCCT GGCCACTCCG GGGGCCACTT CTCCAGCCTG 
GCCAATGGCG CCGCCCAGGA TGGCGACACG CACCCCAGCA CCGGCAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ATGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCCTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTAGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA TGATGAGGAG 
GAGGAGAACA GCGCGTTCGA ATCTGTGCCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGAG 
GAAAGAACCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGAC 
TGGGAAGCTC GAATTGACAG CCACGGGCGG GTCTTTTATG TGGACCACGT GAACCGCACA 
ACCACCTGGC AGCGTCCAAC GGCAGCAGCC ACCCCGGATG GCATGCGGAG ATCGGGGTCC 
ATCCAGCAGA TGGAGCAACT CAACAGGCGG TATCAAAACA TTCAGCGAAC CATTGCAACA 
GAGAGGTCGG AAGAAGATTC TGGCAGCCAA AGCTGCGAGC AAGCCCCAGC AGGAGGAGGC 
GGAGGTGGAG GGAGTGACTC AGAAGCCGAA TCTTCCCAGT CCAGCTTAGA TCTAAGGAGA 
GAAGGGTCAC TTTCTCCGGT TAACTCACAA AAAATCACCT TGCTGCTGCA GTCCCCAGCG 
GTCAAGTTCA TCACCAACCC CGAATTCTTC ACTGTGCTGC ATGCCAATTA TAGTGCCTAC 
CGAGTGTTCA CCAGTAGCAC CTGCTTAAAG CACATGATTC TGAAAGTCCG ACGGGATGCT 
CGCAATTTTG AACGCTACCA GCACAACCGG GACTTGGTGA ATTTCATCAA CATGTTCGCA 
GACACTCGGC TGGAACTGCC CCGGGGCTGG GAGATCAAAA CGGACCAGCA GGGAAAGTCT 
TTTTTCGTGG ACCACAACAG TCGAGCTACC ACTTTCATTG ACCCCCGAAT CCCTCTTCAG 
AACGGTCGTC TTCCCAATCA TCTAACCCAC CGACAGCACC TCCAGAGACT CCGAAGTTAC 
AGCGCTGGAG AGGCCTCAGA AGTTTCTAGA AACAGAGGAG CCTCTTTACT GGCCAGGCCA 
GGACACAGCT TAGTAGCTGC TATTCGAAGC CAACATCAAC ATGAGTCATT GCCACTGGCA 
TATAATGACA AGATTGTGGC ATTTCTTCGC CAGCCAAACA TTTTTGAAAT GCTGCAAGAG 
CGTCAGCCAA GCTTAGCAAG AAACCACACA CTCAGGGAGA AAATCCATTA CATTCGGACT 
GAGGGTAATC ACGGGCTTGA GAAGTTGTCC TGTGATGCGG ATCTGGTCAT TTTGCTGAGT 
CTCTTTGAAG AAGAGATTAT GTCCTACGTC CCCCTGCAGG CTGCCTTCCA CCCTGGGTAT 
AGCTTCTCTC CTCGCTGTTC ACCCTGTTCT TCACCTCAGA ACTCCCCAGG TTTACAGAGA 
GCCAGCGCAA GAGCCCCTTC CCCCTACCGG AGAGACTTTG AGGCCAAGCT CCGCAATTTC 
TACAGAAAAC TGGAAGCCAA AGGATTTGGT CAGGGTCCAG GGAAAATTAA GCTCATTATT 
CGCCGGGATC ATTTGTTGGA GGGAACCTTC AATCAAGTGA TGGCTTATTC GCGGAAAGAG 
CTCCAGCGAA ACAAGCTCTA TGTCACCTTT GTTGGAGAGG AGGGCCTGGA CTACAGTGGC 
CCCTCGCGGG AATTCTTCTT CCTTCTGTCT CAGGAGCTCT TCAACCCTTA CTATGGACTC 
TTTGAGTACT CGGCAAATGA TACTTACACG GTGCAGATCA GCCCCATGTC CGCATTTGTA 
GAAAACCATC TTGAGTGGTT CAGGTTTAGC GGTCGCATCC TGGGCCTGGC TCTGATCCAT 
CAGTACCTTC TTGACGCTTT CTTCACGAGG CCCTTCTACA AGGCACTCCT GAGACTGCCC 
TGTGATTTGA GTGACCTGGA ATATTTGGAT GAGGAATTCC ACCAGAGCTT GCAGTGGATG 
AAGGACAACA ACATCACAGA CATCCTAGAC CTCACTTTCA CCGTTAATGA AGAGGTTTTT 
GGACAGGTCA CGGAAAGGGA GTTGAAGTCT GGAGGAGCCA ACACACAGGT GACGGAGAAG 
AACAAGAAGG AGTACATCGA GCGCATGGTG AAGTGGCGGG TGGAACGCGG CGTGGTACAG 
CAGACCGAGG CACTGGTGCG CGGCTTCTAC GAGGTTGTAG ACTCGAGGCT GGTGTCCGTG 
TTTGATGCCA GGGAGCTGGA GCTGGTGATA GCTGGCACCG CGGAAATCGA CCTAAATGAC 
TGGCGGAATA ACACCGAGTA CCGGGGAGGT TACCACGATG GGCATCTTGT GATCCGCTGG 
TTCTGGGCTG CAGTGGAGCG CTTCAATAAT GAGCAGAGGC TGAGATTACT GCAGTTTGTC 
ACGGGAACAT CCAGCGTGCC CTACGAAGGC TTCGCAGCCC TCCGTGGGAG CAATGGGCTT 
CGGCGCTTCT GCATAGAGAA ATGGGGGAAA ATTACCTCTC TCCCCAGGGC ACACACATGC 
TTCAACCGAC TGGATCTTCC ACCGTATCCC TCGTACTCCA TGTTGTATGA AAAGCTGTTA 
ACAGCAGTAG AGGAAACCAG CACCTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014989019.1
CDS	403..5121
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015133533.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015133533.2

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC GGAGCCTGAG 
TCAGCCCCCA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGATCTGAGG CACCGGAGTC CTCTGAGGGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCTGGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAC 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCGA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCACCAGCC 
AGCACCGAGG AGGAGCCCTT AGAGGAGGAA GCAACGACAC AGAGCCGGGC TGGAAGGGAA 
GAAGAGAAGG AGCAGGAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAAC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCT 
GAGCTGGAGA CAGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCGGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACAGTGGC GGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCATCCT GGCCACTCCG GGGGCCACTT CTCCAGCCTG 
GCCAATGGCG CCGCCCAGGA TGGCGACACG CACCCCAGCA CCGGCAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ATGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCCTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTAGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA TGATGAGGAG 
GAGGAGAACA GCGCGTTCGA ATCTGTGCCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGAG 
GAAAGAACCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGAC 
TGGGAAGCTC GAATTGACAG CCACGGGCGG GTCTTTTATG TGGACCACGT GAACCGCACA 
ACCACCTGGC AGCGTCCAAC GGCAGCAGCC ACCCCGGATG GCATGCGGAG ATCGGGGTCC 
ATCCAGCAGA TGGAGCAACT CAACAGGCGG TATCAAAACA TTCAGCGAAC CATTGCAACA 
GAGAGGTCGG AAGAAGATTC TGGCAGCCAA AGCTGCGAGC AAGCCCCAGC AGGAGGAGGC 
GGAGGTGGAG GGAGTGACTC AGAAGCCGAA TCTTCCCAGT CCAGCTTAGA TCTAAGGAGA 
GAAGGGTCAC TTTCTCCGGT TAACTCACAA AAAATCACCT TGCTGCTGCA GTCCCCAGCG 
GTCAAGTTCA TCACCAACCC CGAATTCTTC ACTGTGCTGC ATGCCAATTA TAGTGCCTAC 
CGAGTGTTCA CCAGTAGCAC CTGCTTAAAG CACATGATTC TGAAAGTCCG ACGGGATGCT 
CGCAATTTTG AACGCTACCA GCACAACCGG GACTTGGTGA ATTTCATCAA CATGTTCGCA 
GACACTCGGC TGGAACTGCC CCGGGGCTGG GAGATCAAAA CGGACCAGCA GGGAAAGTCT 
TTTTTCGTGG ACCACAACAG TCGAGCTACC ACTTTCATTG ACCCCCGAAT CCCTCTTCAG 
AACGGTCGTC TTCCCAATCA TCTAACCCAC CGACAGCACC TCCAGAGACT CCGAAGTTAC 
AGCGCTGGAG AGGCCTCAGA AGTTTCTAGA AACAGAGGAG CCTCTTTACT GGCCAGGCCA 
GGACACAGCT TAGTAGCTGC TATTCGAAGC CAACATCAAC ATGAGTCATT GCCACTGGCA 
TATAATGACA AGATTGTGGC ATTTCTTCGC CAGCCAAACA TTTTTGAAAT GCTGCAAGAG 
CGTCAGCCAA GCTTAGCAAG AAACCACACA CTCAGGGAGA AAATCCATTA CATTCGGACT 
GAGGGTAATC ACGGGCTTGA GAAGTTGTCC TGTGATGCGG ATCTGGTCAT TTTGCTGAGT 
CTCTTTGAAG AAGAGATTAT GTCCTACGTC CCCCTGCAGG CTGCCTTCCA CCCTGGGTAT 
AGCTTCTCTC CTCGCTGTTC ACCCTGTTCT TCACCTCAGA ACTCCCCAGG TTTACAGAGA 
GCCAGCGCAA GAGCCCCTTC CCCCTACCGG AGAGACTTTG AGGCCAAGCT CCGCAATTTC 
TACAGAAAAC TGGAAGCCAA AGGATTTGGT CAGGGTCCAG GGAAAATTAA GCTCATTATT 
CGCCGGGATC ATTTGTTGGA GGGAACCTTC AATCAAGTGA TGGCTTATTC GCGGAAAGAG 
CTCCAGCGAA ACAAGCTCTA TGTCACCTTT GTTGGAGAGG AGGGCCTGGA CTACAGTGGC 
CCCTCGCGGG AATTCTTCTT CCTTCTGTCT CAGGAGCTCT TCAACCCTTA CTATGGACTC 
TTTGAGTACT CGGCAAATGA TACTTACACG GTGCAGATCA GCCCCATGTC CGCATTTGTA 
GAAAACCATC TTGAGTGGTT CAGGTTTAGC GGTCGCATCC TGGGCCTGGC TCTGATCCAT 
CAGTACCTTC TTGACGCTTT CTTCACGAGG CCCTTCTACA AGGCACTCCT GAGACTGCCC 
TGTGATTTGA GTGACCTGGA ATATTTGGAT GAGGAATTCC ACCAGAGCTT GCAGTGGATG 
AAGGACAACA ACATCACAGA CATCCTAGAC CTCACTTTCA CCGTTAATGA AGAGGTTTTT 
GGACAGGTCA CGGAAAGGGA GTTGAAGTCT GGAGGAGCCA ACACACAGGT GACGGAGAAG 
AACAAGAAGG AGTACATCGA GCGCATGGTG AAGTGGCGGG TGGAACGCGG CGTGGTACAG 
CAGACCGAGG CACTGGTGCG CGGCTTCTAC GAGGTTGTAG ACTCGAGGCT GGTGTCCGTG 
TTTGATGCCA GGGAGCTGGA GCTGGTGATA GCTGGCACCG CGGAAATCGA CCTAAATGAC 
TGGCGGAATA ACACCGAGTA CCGGGGAGGT TACCACGATG GGCATCTTGT GATCCGCTGG 
TTCTGGGCTG CAGTGGAGCG CTTCAATAAT GAGCAGAGGC TGAGATTACT GCAGTTTGTC 
ACGGGAACAT CCAGCGTGCC CTACGAAGGC TTCGCAGCCC TCCGTGGGAG CAATGGGCTT 
CGGCGCTTCT GCATAGAGAA ATGGGGGAAA ATTACCTCTC TCCCCAGGGC ACACACATGC 
TTCAACCGAC TGGATCTTCC ACCGTATCCC TCGTACTCCA TGTTGTATGA AAAGCTGTTA 
ACAGCAGTAG AGGAAACCAG CACCTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( NM_001265810.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> NP_001252739.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), mRNA.

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CloneID	OMb03739
Clone ID Related Accession (Same CDS sequence)	NM_001265810.1
Accession Version	NM_001265810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JV565083.1. On or before May 25, 2012 this sequence version replaced XM_001096775.1, XM_001096889.2, XM_002803301.1. ##Evidence-Data-START## Transcript exon combination :: JV565083.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA4688315, SAMEA4688316 [ECO:0000350] ##Evidence-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC GGAGCCTGAG 
TCAGCCCCCA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGATCTGAGG CACCGGAGTC CTCTGAGGGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCTGGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAC 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCGA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCACCAGCC 
AGCACCGAGG AGGAGCCCTT AGAGGAGGAA GCAACGACAC AGAGCCGGGC TGGAAGGGAA 
GAAGAGAAGG AGCAGGAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAAC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCT 
GAGCTGGAGA CAGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCGGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACAGTGGC GGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCATCCT GGCCACTCCG GGGGCCACTT CTCCAGCCTG 
GCCAATGGCG CCGCCCAGGA TGGCGACACG CACCCCAGCA CCGGCAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ATGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCCTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTAGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA TGATGAGGAG 
GAGGAGAACA GCGCGTTCGA ATCTGTGCCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGAG 
GAAAGAACCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC GGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCG 
GTTAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAATTCT TCACTGTGCT GCATGCCAAT TATAGTGCCT ACCGAGTGTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACCC ACCGACAGCA CCTCCAGAGA CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCTCGCTGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGCGC AAGAGCCCCT 
TCCCCCTACC GGAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC AGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAAGT GATGGCTTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TATGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAATTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGCCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGC TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCCTAG ACCTCACTTT CACCGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACGGAGA AGAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAACGC GGCGTGGTAC AGCAGACCGA GGCACTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACCGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCAGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACCTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001252739.1
CDS	272..5092
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	NM_001265810.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001265810.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCAGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC GGAGCCTGAG 
TCAGCCCCCA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGATCTGAGG CACCGGAGTC CTCTGAGGGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCTGGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAC 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCGA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCACCAGCC 
AGCACCGAGG AGGAGCCCTT AGAGGAGGAA GCAACGACAC AGAGCCGGGC TGGAAGGGAA 
GAAGAGAAGG AGCAGGAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAAC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCT 
GAGCTGGAGA CAGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCGGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACAGTGGC GGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCATCCT GGCCACTCCG GGGGCCACTT CTCCAGCCTG 
GCCAATGGCG CCGCCCAGGA TGGCGACACG CACCCCAGCA CCGGCAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ATGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCCTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTAGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA TGATGAGGAG 
GAGGAGAACA GCGCGTTCGA ATCTGTGCCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGAG 
GAAAGAACCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC GGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCG 
GTTAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAATTCT TCACTGTGCT GCATGCCAAT TATAGTGCCT ACCGAGTGTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACCC ACCGACAGCA CCTCCAGAGA CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCTCGCTGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGCGC AAGAGCCCCT 
TCCCCCTACC GGAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC AGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAAGT GATGGCTTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TATGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAATTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGCCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGC TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCCTAG ACCTCACTTT CACCGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACGGAGA AGAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAACGC GGCGTGGTAC AGCAGACCGA GGCACTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACCGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCAGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACCTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015133526.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014989012.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMb30252
Clone ID Related Accession (Same CDS sequence)	XM_015133526.1
Accession Version	XM_015133526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014804724.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCTCTC 
CGATACAGCT ACAATCCTGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCATGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTACTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATCGGCGCTG ATGAGACTGT ACAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACGGAG 
CCTGAGTCAG CCCCCATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGAT CTGAGGCACC GGAGTCCTCT GAGGGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC TGGATGGTCC AGGGAACCAA AGCATGGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCACGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGACCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCGAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCG TCAGCACTGC TGCTGGAAGA CGGTGAAGCA 
CCAGCCAGCA CCGAGGAGGA GCCCTTAGAG GAGGAAGCAA CGACACAGAG CCGGGCTGGA 
AGGGAAGAAG AGAAGGAGCA GGAGGAGGAG GAGGGAGATG TGTCTACCCT GGAGCAGGGA 
GAGGGCAGGC TGCAACTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCTGAGC TGGAGACAGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC AGTGGCGGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CATCCTGGCC ACTCCGGGGG CCACTTCTCC 
AGCCTGGCCA ATGGCGCCGC CCAGGATGGC GACACGCACC CCAGCACCGG CAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGATGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCCT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TAGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGATGAT 
GAGGAGGAGG AGAACAGCGC GTTCGAATCT GTGCCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGAGGAAA GAACCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGGTGAAT GTCCTATACT CCATAATTCC CAGCCAGTAA GCCAGCTTCC TTCCCTGAGG 
CCTGAACATC ATCACTACCC AACAATCGAT GAGCCTCTTC CACCAAACTG GGAAGCTCGA 
ATTGACAGCC ACGGGCGGGT CTTTTATGTG GACCACGTGA ACCGCACAAC CACCTGGCAG 
CGTCCAACGG CAGCAGCCAC CCCGGATGGC ATGCGGAGAT CGGGGTCCAT CCAGCAGATG 
GAGCAACTCA ACAGGCGGTA TCAAAACATT CAGCGAACCA TTGCAACAGA GAGGTCGGAA 
GAAGATTCTG GCAGCCAAAG CTGCGAGCAA GCCCCAGCAG GAGGAGGCGG AGGTGGAGGG 
AGTGACTCAG AAGCCGAATC TTCCCAGTCC AGCTTAGATC TAAGGAGAGA AGGGTCACTT 
TCTCCGGTTA ACTCACAAAA AATCACCTTG CTGCTGCAGT CCCCAGCGGT CAAGTTCATC 
ACCAACCCCG AATTCTTCAC TGTGCTGCAT GCCAATTATA GTGCCTACCG AGTGTTCACC 
AGTAGCACCT GCTTAAAGCA CATGATTCTG AAAGTCCGAC GGGATGCTCG CAATTTTGAA 
CGCTACCAGC ACAACCGGGA CTTGGTGAAT TTCATCAACA TGTTCGCAGA CACTCGGCTG 
GAACTGCCCC GGGGCTGGGA GATCAAAACG GACCAGCAGG GAAAGTCTTT TTTCGTGGAC 
CACAACAGTC GAGCTACCAC TTTCATTGAC CCCCGAATCC CTCTTCAGAA CGGTCGTCTT 
CCCAATCATC TAACCCACCG ACAGCACCTC CAGAGACTCC GAAGTTACAG CGCTGGAGAG 
GCCTCAGAAG TTTCTAGAAA CAGAGGAGCC TCTTTACTGG CCAGGCCAGG ACACAGCTTA 
GTAGCTGCTA TTCGAAGCCA ACATCAACAT GAGTCATTGC CACTGGCATA TAATGACAAG 
ATTGTGGCAT TTCTTCGCCA GCCAAACATT TTTGAAATGC TGCAAGAGCG TCAGCCAAGC 
TTAGCAAGAA ACCACACACT CAGGGAGAAA ATCCATTACA TTCGGACTGA GGGTAATCAC 
GGGCTTGAGA AGTTGTCCTG TGATGCGGAT CTGGTCATTT TGCTGAGTCT CTTTGAAGAA 
GAGATTATGT CCTACGTCCC CCTGCAGGCT GCCTTCCACC CTGGGTATAG CTTCTCTCCT 
CGCTGTTCAC CCTGTTCTTC ACCTCAGAAC TCCCCAGGTT TACAGAGAGC CAGCGCAAGA 
GCCCCTTCCC CCTACCGGAG AGACTTTGAG GCCAAGCTCC GCAATTTCTA CAGAAAACTG 
GAAGCCAAAG GATTTGGTCA GGGTCCAGGG AAAATTAAGC TCATTATTCG CCGGGATCAT 
TTGTTGGAGG GAACCTTCAA TCAAGTGATG GCTTATTCGC GGAAAGAGCT CCAGCGAAAC 
AAGCTCTATG TCACCTTTGT TGGAGAGGAG GGCCTGGACT ACAGTGGCCC CTCGCGGGAA 
TTCTTCTTCC TTCTGTCTCA GGAGCTCTTC AACCCTTACT ATGGACTCTT TGAGTACTCG 
GCAAATGATA CTTACACGGT GCAGATCAGC CCCATGTCCG CATTTGTAGA AAACCATCTT 
GAGTGGTTCA GGTTTAGCGG TCGCATCCTG GGCCTGGCTC TGATCCATCA GTACCTTCTT 
GACGCTTTCT TCACGAGGCC CTTCTACAAG GCACTCCTGA GACTGCCCTG TGATTTGAGT 
GACCTGGAAT ATTTGGATGA GGAATTCCAC CAGAGCTTGC AGTGGATGAA GGACAACAAC 
ATCACAGACA TCCTAGACCT CACTTTCACC GTTAATGAAG AGGTTTTTGG ACAGGTCACG 
GAAAGGGAGT TGAAGTCTGG AGGAGCCAAC ACACAGGTGA CGGAGAAGAA CAAGAAGGAG 
TACATCGAGC GCATGGTGAA GTGGCGGGTG GAACGCGGCG TGGTACAGCA GACCGAGGCA 
CTGGTGCGCG GCTTCTACGA GGTTGTAGAC TCGAGGCTGG TGTCCGTGTT TGATGCCAGG 
GAGCTGGAGC TGGTGATAGC TGGCACCGCG GAAATCGACC TAAATGACTG GCGGAATAAC 
ACCGAGTACC GGGGAGGTTA CCACGATGGG CATCTTGTGA TCCGCTGGTT CTGGGCTGCA 
GTGGAGCGCT TCAATAATGA GCAGAGGCTG AGATTACTGC AGTTTGTCAC GGGAACATCC 
AGCGTGCCCT ACGAAGGCTT CGCAGCCCTC CGTGGGAGCA ATGGGCTTCG GCGCTTCTGC 
ATAGAGAAAT GGGGGAAAAT TACCTCTCTC CCCAGGGCAC ACACATGCTT CAACCGACTG 
GATCTTCCAC CGTATCCCTC GTACTCCATG TTGTATGAAA AGCTGTTAAC AGCAGTAGAG 
GAAACCAGCA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014989012.1
CDS	340..5166
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015133526.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015133526.1

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCTCTC 
CGATACAGCT ACAATCCTGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCATGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTACTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATCGGCGCTG ATGAGACTGT ACAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACGGAG 
CCTGAGTCAG CCCCCATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGAT CTGAGGCACC GGAGTCCTCT GAGGGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC TGGATGGTCC AGGGAACCAA AGCATGGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCACGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGACCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCGAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCG TCAGCACTGC TGCTGGAAGA CGGTGAAGCA 
CCAGCCAGCA CCGAGGAGGA GCCCTTAGAG GAGGAAGCAA CGACACAGAG CCGGGCTGGA 
AGGGAAGAAG AGAAGGAGCA GGAGGAGGAG GAGGGAGATG TGTCTACCCT GGAGCAGGGA 
GAGGGCAGGC TGCAACTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCTGAGC TGGAGACAGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC AGTGGCGGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CATCCTGGCC ACTCCGGGGG CCACTTCTCC 
AGCCTGGCCA ATGGCGCCGC CCAGGATGGC GACACGCACC CCAGCACCGG CAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGATGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCCT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TAGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGATGAT 
GAGGAGGAGG AGAACAGCGC GTTCGAATCT GTGCCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGAGGAAA GAACCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGGTGAAT GTCCTATACT CCATAATTCC CAGCCAGTAA GCCAGCTTCC TTCCCTGAGG 
CCTGAACATC ATCACTACCC AACAATCGAT GAGCCTCTTC CACCAAACTG GGAAGCTCGA 
ATTGACAGCC ACGGGCGGGT CTTTTATGTG GACCACGTGA ACCGCACAAC CACCTGGCAG 
CGTCCAACGG CAGCAGCCAC CCCGGATGGC ATGCGGAGAT CGGGGTCCAT CCAGCAGATG 
GAGCAACTCA ACAGGCGGTA TCAAAACATT CAGCGAACCA TTGCAACAGA GAGGTCGGAA 
GAAGATTCTG GCAGCCAAAG CTGCGAGCAA GCCCCAGCAG GAGGAGGCGG AGGTGGAGGG 
AGTGACTCAG AAGCCGAATC TTCCCAGTCC AGCTTAGATC TAAGGAGAGA AGGGTCACTT 
TCTCCGGTTA ACTCACAAAA AATCACCTTG CTGCTGCAGT CCCCAGCGGT CAAGTTCATC 
ACCAACCCCG AATTCTTCAC TGTGCTGCAT GCCAATTATA GTGCCTACCG AGTGTTCACC 
AGTAGCACCT GCTTAAAGCA CATGATTCTG AAAGTCCGAC GGGATGCTCG CAATTTTGAA 
CGCTACCAGC ACAACCGGGA CTTGGTGAAT TTCATCAACA TGTTCGCAGA CACTCGGCTG 
GAACTGCCCC GGGGCTGGGA GATCAAAACG GACCAGCAGG GAAAGTCTTT TTTCGTGGAC 
CACAACAGTC GAGCTACCAC TTTCATTGAC CCCCGAATCC CTCTTCAGAA CGGTCGTCTT 
CCCAATCATC TAACCCACCG ACAGCACCTC CAGAGACTCC GAAGTTACAG CGCTGGAGAG 
GCCTCAGAAG TTTCTAGAAA CAGAGGAGCC TCTTTACTGG CCAGGCCAGG ACACAGCTTA 
GTAGCTGCTA TTCGAAGCCA ACATCAACAT GAGTCATTGC CACTGGCATA TAATGACAAG 
ATTGTGGCAT TTCTTCGCCA GCCAAACATT TTTGAAATGC TGCAAGAGCG TCAGCCAAGC 
TTAGCAAGAA ACCACACACT CAGGGAGAAA ATCCATTACA TTCGGACTGA GGGTAATCAC 
GGGCTTGAGA AGTTGTCCTG TGATGCGGAT CTGGTCATTT TGCTGAGTCT CTTTGAAGAA 
GAGATTATGT CCTACGTCCC CCTGCAGGCT GCCTTCCACC CTGGGTATAG CTTCTCTCCT 
CGCTGTTCAC CCTGTTCTTC ACCTCAGAAC TCCCCAGGTT TACAGAGAGC CAGCGCAAGA 
GCCCCTTCCC CCTACCGGAG AGACTTTGAG GCCAAGCTCC GCAATTTCTA CAGAAAACTG 
GAAGCCAAAG GATTTGGTCA GGGTCCAGGG AAAATTAAGC TCATTATTCG CCGGGATCAT 
TTGTTGGAGG GAACCTTCAA TCAAGTGATG GCTTATTCGC GGAAAGAGCT CCAGCGAAAC 
AAGCTCTATG TCACCTTTGT TGGAGAGGAG GGCCTGGACT ACAGTGGCCC CTCGCGGGAA 
TTCTTCTTCC TTCTGTCTCA GGAGCTCTTC AACCCTTACT ATGGACTCTT TGAGTACTCG 
GCAAATGATA CTTACACGGT GCAGATCAGC CCCATGTCCG CATTTGTAGA AAACCATCTT 
GAGTGGTTCA GGTTTAGCGG TCGCATCCTG GGCCTGGCTC TGATCCATCA GTACCTTCTT 
GACGCTTTCT TCACGAGGCC CTTCTACAAG GCACTCCTGA GACTGCCCTG TGATTTGAGT 
GACCTGGAAT ATTTGGATGA GGAATTCCAC CAGAGCTTGC AGTGGATGAA GGACAACAAC 
ATCACAGACA TCCTAGACCT CACTTTCACC GTTAATGAAG AGGTTTTTGG ACAGGTCACG 
GAAAGGGAGT TGAAGTCTGG AGGAGCCAAC ACACAGGTGA CGGAGAAGAA CAAGAAGGAG 
TACATCGAGC GCATGGTGAA GTGGCGGGTG GAACGCGGCG TGGTACAGCA GACCGAGGCA 
CTGGTGCGCG GCTTCTACGA GGTTGTAGAC TCGAGGCTGG TGTCCGTGTT TGATGCCAGG 
GAGCTGGAGC TGGTGATAGC TGGCACCGCG GAAATCGACC TAAATGACTG GCGGAATAAC 
ACCGAGTACC GGGGAGGTTA CCACGATGGG CATCTTGTGA TCCGCTGGTT CTGGGCTGCA 
GTGGAGCGCT TCAATAATGA GCAGAGGCTG AGATTACTGC AGTTTGTCAC GGGAACATCC 
AGCGTGCCCT ACGAAGGCTT CGCAGCCCTC CGTGGGAGCA ATGGGCTTCG GCGCTTCTGC 
ATAGAGAAAT GGGGGAAAAT TACCTCTCTC CCCAGGGCAC ACACATGCTT CAACCGACTG 
GATCTTCCAC CGTATCCCTC GTACTCCATG TTGTATGAAA AGCTGTTAAC AGCAGTAGAG 
GAAACCAGCA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015133527.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014989013.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMb30253
Clone ID Related Accession (Same CDS sequence)	XM_015133527.1
Accession Version	XM_015133527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014804724.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCTCTC 
CGATACAGCT ACAATCCTGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCATGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTACTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATCGGCGCTG ATGAGACTGT ACAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCAACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCGGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCGGTTAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAAT TCTTCACTGT GCTGCATGCC AATTATAGTG CCTACCGAGT GTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CCCACCGACA GCACCTCCAG AGACTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCTCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC 
CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA AGTGATGGCT TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTATGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGCTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCC TAGACCTCAC TTTCACCGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA CGCGGCGTGG TACAGCAGAC CGAGGCACTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCAGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC CTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014989013.1
CDS	340..5163
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015133527.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015133527.1

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCTCTC 
CGATACAGCT ACAATCCTGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCATGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTACTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATCGGCGCTG ATGAGACTGT ACAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCAACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCGGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCGGTTAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAAT TCTTCACTGT GCTGCATGCC AATTATAGTG CCTACCGAGT GTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CCCACCGACA GCACCTCCAG AGACTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCTCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC 
CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA AGTGATGGCT TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTATGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGCTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCC TAGACCTCAC TTTCACCGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA CGCGGCGTGG TACAGCAGAC CGAGGCACTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCAGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC CTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015133529.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014989015.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

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CloneID	OMb30254
Clone ID Related Accession (Same CDS sequence)	XM_015133529.1 , XM_015133530.1
Accession Version	XM_015133529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014804724.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCAACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCGGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCGGTTAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAAT TCTTCACTGT GCTGCATGCC AATTATAGTG CCTACCGAGT GTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CCCACCGACA GCACCTCCAG AGACTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCTCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC 
CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA AGTGATGGCT TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTATGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGCTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCC TAGACCTCAC TTTCACCGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA CGCGGCGTGG TACAGCAGAC CGAGGCACTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCAGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC CTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014989015.1
CDS	679..5502
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015133529.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015133529.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCAACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCGGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCGGTTAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAAT TCTTCACTGT GCTGCATGCC AATTATAGTG CCTACCGAGT GTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CCCACCGACA GCACCTCCAG AGACTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCTCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC 
CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA AGTGATGGCT TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTATGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGCTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCC TAGACCTCAC TTTCACCGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA CGCGGCGTGG TACAGCAGAC CGAGGCACTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCAGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC CTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015133530.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014989016.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

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CloneID	OMb30254
Clone ID Related Accession (Same CDS sequence)	XM_015133529.1 , XM_015133530.1
Accession Version	XM_015133530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014804724.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCAACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCGGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCGGTTAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAAT TCTTCACTGT GCTGCATGCC AATTATAGTG CCTACCGAGT GTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CCCACCGACA GCACCTCCAG AGACTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCTCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC 
CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA AGTGATGGCT TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTATGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGCTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCC TAGACCTCAC TTTCACCGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA CGCGGCGTGG TACAGCAGAC CGAGGCACTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCAGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC CTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014989016.1
CDS	359..5182
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015133530.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015133530.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCAACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCGGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCGGTTAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAAT TCTTCACTGT GCTGCATGCC AATTATAGTG CCTACCGAGT GTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CCCACCGACA GCACCTCCAG AGACTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCTCGC 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC 
CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA AGTGATGGCT TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTATGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAATTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGC CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGCTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCC TAGACCTCAC TTTCACCGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAGAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA CGCGGCGTGG TACAGCAGAC CGAGGCACTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACC 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCAGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC CTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015133531.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014989017.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

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CloneID	OMb30255
Clone ID Related Accession (Same CDS sequence)	XM_015133531.1
Accession Version	XM_015133531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014804724.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCTCTC 
CGATACAGCT ACAATCCTGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCATGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTACTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATCGGCGCTG ATGAGACTGT ACAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACGGAG 
CCTGAGTCAG CCCCCATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGAT CTGAGGCACC GGAGTCCTCT GAGGGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC TGGATGGTCC AGGGAACCAA AGCATGGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCACGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGACCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCGAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCG TCAGCACTGC TGCTGGAAGA CGGTGAAGCA 
CCAGCCAGCA CCGAGGAGGA GCCCTTAGAG GAGGAAGCAA CGACACAGAG CCGGGCTGGA 
AGGGAAGAAG AGAAGGAGCA GGAGGAGGAG GAGGGAGATG TGTCTACCCT GGAGCAGGGA 
GAGGGCAGGC TGCAACTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCTGAGC TGGAGACAGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC AGTGGCGGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CATCCTGGCC ACTCCGGGGG CCACTTCTCC 
AGCCTGGCCA ATGGCGCCGC CCAGGATGGC GACACGCACC CCAGCACCGG CAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGATGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCCT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TAGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGATGAT 
GAGGAGGAGG AGAACAGCGC GTTCGAATCT GTGCCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGAGGAAA GAACCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGACTGGG AAGCTCGAAT TGACAGCCAC GGGCGGGTCT TTTATGTGGA CCACGTGAAC 
CGCACAACCA CCTGGCAGCG TCCAACGGCA GCAGCCACCC CGGATGGCAT GCGGAGATCG 
GGGTCCATCC AGCAGATGGA GCAACTCAAC AGGCGGTATC AAAACATTCA GCGAACCATT 
GCAACAGAGA GGTCGGAAGA AGATTCTGGC AGCCAAAGCT GCGAGCAAGC CCCAGCAGGA 
GGAGGCGGAG GTGGAGGGAG TGACTCAGAA GCCGAATCTT CCCAGTCCAG CTTAGATCTA 
AGGAGAGAAG GGTCACTTTC TCCGGTTAAC TCACAAAAAA TCACCTTGCT GCTGCAGTCC 
CCAGCGGTCA AGTTCATCAC CAACCCCGAA TTCTTCACTG TGCTGCATGC CAATTATAGT 
GCCTACCGAG TGTTCACCAG TAGCACCTGC TTAAAGCACA TGATTCTGAA AGTCCGACGG 
GATGCTCGCA ATTTTGAACG CTACCAGCAC AACCGGGACT TGGTGAATTT CATCAACATG 
TTCGCAGACA CTCGGCTGGA ACTGCCCCGG GGCTGGGAGA TCAAAACGGA CCAGCAGGGA 
AAGTCTTTTT TCGTGGACCA CAACAGTCGA GCTACCACTT TCATTGACCC CCGAATCCCT 
CTTCAGAACG GTCGTCTTCC CAATCATCTA ACCCACCGAC AGCACCTCCA GAGACTCCGA 
AGTTACAGCG CTGGAGAGGC CTCAGAAGTT TCTAGAAACA GAGGAGCCTC TTTACTGGCC 
AGGCCAGGAC ACAGCTTAGT AGCTGCTATT CGAAGCCAAC ATCAACATGA GTCATTGCCA 
CTGGCATATA ATGACAAGAT TGTGGCATTT CTTCGCCAGC CAAACATTTT TGAAATGCTG 
CAAGAGCGTC AGCCAAGCTT AGCAAGAAAC CACACACTCA GGGAGAAAAT CCATTACATT 
CGGACTGAGG GTAATCACGG GCTTGAGAAG TTGTCCTGTG ATGCGGATCT GGTCATTTTG 
CTGAGTCTCT TTGAAGAAGA GATTATGTCC TACGTCCCCC TGCAGGCTGC CTTCCACCCT 
GGGTATAGCT TCTCTCCTCG CTGTTCACCC TGTTCTTCAC CTCAGAACTC CCCAGGTTTA 
CAGAGAGCCA GCGCAAGAGC CCCTTCCCCC TACCGGAGAG ACTTTGAGGC CAAGCTCCGC 
AATTTCTACA GAAAACTGGA AGCCAAAGGA TTTGGTCAGG GTCCAGGGAA AATTAAGCTC 
ATTATTCGCC GGGATCATTT GTTGGAGGGA ACCTTCAATC AAGTGATGGC TTATTCGCGG 
AAAGAGCTCC AGCGAAACAA GCTCTATGTC ACCTTTGTTG GAGAGGAGGG CCTGGACTAC 
AGTGGCCCCT CGCGGGAATT CTTCTTCCTT CTGTCTCAGG AGCTCTTCAA CCCTTACTAT 
GGACTCTTTG AGTACTCGGC AAATGATACT TACACGGTGC AGATCAGCCC CATGTCCGCA 
TTTGTAGAAA ACCATCTTGA GTGGTTCAGG TTTAGCGGTC GCATCCTGGG CCTGGCTCTG 
ATCCATCAGT ACCTTCTTGA CGCTTTCTTC ACGAGGCCCT TCTACAAGGC ACTCCTGAGA 
CTGCCCTGTG ATTTGAGTGA CCTGGAATAT TTGGATGAGG AATTCCACCA GAGCTTGCAG 
TGGATGAAGG ACAACAACAT CACAGACATC CTAGACCTCA CTTTCACCGT TAATGAAGAG 
GTTTTTGGAC AGGTCACGGA AAGGGAGTTG AAGTCTGGAG GAGCCAACAC ACAGGTGACG 
GAGAAGAACA AGAAGGAGTA CATCGAGCGC ATGGTGAAGT GGCGGGTGGA ACGCGGCGTG 
GTACAGCAGA CCGAGGCACT GGTGCGCGGC TTCTACGAGG TTGTAGACTC GAGGCTGGTG 
TCCGTGTTTG ATGCCAGGGA GCTGGAGCTG GTGATAGCTG GCACCGCGGA AATCGACCTA 
AATGACTGGC GGAATAACAC CGAGTACCGG GGAGGTTACC ACGATGGGCA TCTTGTGATC 
CGCTGGTTCT GGGCTGCAGT GGAGCGCTTC AATAATGAGC AGAGGCTGAG ATTACTGCAG 
TTTGTCACGG GAACATCCAG CGTGCCCTAC GAAGGCTTCG CAGCCCTCCG TGGGAGCAAT 
GGGCTTCGGC GCTTCTGCAT AGAGAAATGG GGGAAAATTA CCTCTCTCCC CAGGGCACAC 
ACATGCTTCA ACCGACTGGA TCTTCCACCG TATCCCTCGT ACTCCATGTT GTATGAAAAG 
CTGTTAACAG CAGTAGAGGA AACCAGCACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014989017.1
CDS	340..5064
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015133531.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015133531.1

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCTCTC 
CGATACAGCT ACAATCCTGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCATGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTACTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATCGGCGCTG ATGAGACTGT ACAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACGGAG 
CCTGAGTCAG CCCCCATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGAT CTGAGGCACC GGAGTCCTCT GAGGGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC TGGATGGTCC AGGGAACCAA AGCATGGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCACGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGACCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCGAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCG TCAGCACTGC TGCTGGAAGA CGGTGAAGCA 
CCAGCCAGCA CCGAGGAGGA GCCCTTAGAG GAGGAAGCAA CGACACAGAG CCGGGCTGGA 
AGGGAAGAAG AGAAGGAGCA GGAGGAGGAG GAGGGAGATG TGTCTACCCT GGAGCAGGGA 
GAGGGCAGGC TGCAACTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCTGAGC TGGAGACAGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CGGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC AGTGGCGGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CATCCTGGCC ACTCCGGGGG CCACTTCTCC 
AGCCTGGCCA ATGGCGCCGC CCAGGATGGC GACACGCACC CCAGCACCGG CAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGATGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCCT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TAGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGATGAT 
GAGGAGGAGG AGAACAGCGC GTTCGAATCT GTGCCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGAGGAAA GAACCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGACTGGG AAGCTCGAAT TGACAGCCAC GGGCGGGTCT TTTATGTGGA CCACGTGAAC 
CGCACAACCA CCTGGCAGCG TCCAACGGCA GCAGCCACCC CGGATGGCAT GCGGAGATCG 
GGGTCCATCC AGCAGATGGA GCAACTCAAC AGGCGGTATC AAAACATTCA GCGAACCATT 
GCAACAGAGA GGTCGGAAGA AGATTCTGGC AGCCAAAGCT GCGAGCAAGC CCCAGCAGGA 
GGAGGCGGAG GTGGAGGGAG TGACTCAGAA GCCGAATCTT CCCAGTCCAG CTTAGATCTA 
AGGAGAGAAG GGTCACTTTC TCCGGTTAAC TCACAAAAAA TCACCTTGCT GCTGCAGTCC 
CCAGCGGTCA AGTTCATCAC CAACCCCGAA TTCTTCACTG TGCTGCATGC CAATTATAGT 
GCCTACCGAG TGTTCACCAG TAGCACCTGC TTAAAGCACA TGATTCTGAA AGTCCGACGG 
GATGCTCGCA ATTTTGAACG CTACCAGCAC AACCGGGACT TGGTGAATTT CATCAACATG 
TTCGCAGACA CTCGGCTGGA ACTGCCCCGG GGCTGGGAGA TCAAAACGGA CCAGCAGGGA 
AAGTCTTTTT TCGTGGACCA CAACAGTCGA GCTACCACTT TCATTGACCC CCGAATCCCT 
CTTCAGAACG GTCGTCTTCC CAATCATCTA ACCCACCGAC AGCACCTCCA GAGACTCCGA 
AGTTACAGCG CTGGAGAGGC CTCAGAAGTT TCTAGAAACA GAGGAGCCTC TTTACTGGCC 
AGGCCAGGAC ACAGCTTAGT AGCTGCTATT CGAAGCCAAC ATCAACATGA GTCATTGCCA 
CTGGCATATA ATGACAAGAT TGTGGCATTT CTTCGCCAGC CAAACATTTT TGAAATGCTG 
CAAGAGCGTC AGCCAAGCTT AGCAAGAAAC CACACACTCA GGGAGAAAAT CCATTACATT 
CGGACTGAGG GTAATCACGG GCTTGAGAAG TTGTCCTGTG ATGCGGATCT GGTCATTTTG 
CTGAGTCTCT TTGAAGAAGA GATTATGTCC TACGTCCCCC TGCAGGCTGC CTTCCACCCT 
GGGTATAGCT TCTCTCCTCG CTGTTCACCC TGTTCTTCAC CTCAGAACTC CCCAGGTTTA 
CAGAGAGCCA GCGCAAGAGC CCCTTCCCCC TACCGGAGAG ACTTTGAGGC CAAGCTCCGC 
AATTTCTACA GAAAACTGGA AGCCAAAGGA TTTGGTCAGG GTCCAGGGAA AATTAAGCTC 
ATTATTCGCC GGGATCATTT GTTGGAGGGA ACCTTCAATC AAGTGATGGC TTATTCGCGG 
AAAGAGCTCC AGCGAAACAA GCTCTATGTC ACCTTTGTTG GAGAGGAGGG CCTGGACTAC 
AGTGGCCCCT CGCGGGAATT CTTCTTCCTT CTGTCTCAGG AGCTCTTCAA CCCTTACTAT 
GGACTCTTTG AGTACTCGGC AAATGATACT TACACGGTGC AGATCAGCCC CATGTCCGCA 
TTTGTAGAAA ACCATCTTGA GTGGTTCAGG TTTAGCGGTC GCATCCTGGG CCTGGCTCTG 
ATCCATCAGT ACCTTCTTGA CGCTTTCTTC ACGAGGCCCT TCTACAAGGC ACTCCTGAGA 
CTGCCCTGTG ATTTGAGTGA CCTGGAATAT TTGGATGAGG AATTCCACCA GAGCTTGCAG 
TGGATGAAGG ACAACAACAT CACAGACATC CTAGACCTCA CTTTCACCGT TAATGAAGAG 
GTTTTTGGAC AGGTCACGGA AAGGGAGTTG AAGTCTGGAG GAGCCAACAC ACAGGTGACG 
GAGAAGAACA AGAAGGAGTA CATCGAGCGC ATGGTGAAGT GGCGGGTGGA ACGCGGCGTG 
GTACAGCAGA CCGAGGCACT GGTGCGCGGC TTCTACGAGG TTGTAGACTC GAGGCTGGTG 
TCCGTGTTTG ATGCCAGGGA GCTGGAGCTG GTGATAGCTG GCACCGCGGA AATCGACCTA 
AATGACTGGC GGAATAACAC CGAGTACCGG GGAGGTTACC ACGATGGGCA TCTTGTGATC 
CGCTGGTTCT GGGCTGCAGT GGAGCGCTTC AATAATGAGC AGAGGCTGAG ATTACTGCAG 
TTTGTCACGG GAACATCCAG CGTGCCCTAC GAAGGCTTCG CAGCCCTCCG TGGGAGCAAT 
GGGCTTCGGC GCTTCTGCAT AGAGAAATGG GGGAAAATTA CCTCTCTCCC CAGGGCACAC 
ACATGCTTCA ACCGACTGGA TCTTCCACCG TATCCCTCGT ACTCCATGTT GTATGAAAAG 
CTGTTAACAG CAGTAGAGGA AACCAGCACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015133532.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014989018.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMb30256
Clone ID Related Accession (Same CDS sequence)	XM_015133532.1
Accession Version	XM_015133532.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014804724.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCTCTC 
CGATACAGCT ACAATCCTGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCATGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTACTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATCGGCGCTG ATGAGACTGT ACAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GACTGGGAAG CTCGAATTGA CAGCCACGGG CGGGTCTTTT ATGTGGACCA CGTGAACCGC 
ACAACCACCT GGCAGCGTCC AACGGCAGCA GCCACCCCGG ATGGCATGCG GAGATCGGGG 
TCCATCCAGC AGATGGAGCA ACTCAACAGG CGGTATCAAA ACATTCAGCG AACCATTGCA 
ACAGAGAGGT CGGAAGAAGA TTCTGGCAGC CAAAGCTGCG AGCAAGCCCC AGCAGGAGGA 
GGCGGAGGTG GAGGGAGTGA CTCAGAAGCC GAATCTTCCC AGTCCAGCTT AGATCTAAGG 
AGAGAAGGGT CACTTTCTCC GGTTAACTCA CAAAAAATCA CCTTGCTGCT GCAGTCCCCA 
GCGGTCAAGT TCATCACCAA CCCCGAATTC TTCACTGTGC TGCATGCCAA TTATAGTGCC 
TACCGAGTGT TCACCAGTAG CACCTGCTTA AAGCACATGA TTCTGAAAGT CCGACGGGAT 
GCTCGCAATT TTGAACGCTA CCAGCACAAC CGGGACTTGG TGAATTTCAT CAACATGTTC 
GCAGACACTC GGCTGGAACT GCCCCGGGGC TGGGAGATCA AAACGGACCA GCAGGGAAAG 
TCTTTTTTCG TGGACCACAA CAGTCGAGCT ACCACTTTCA TTGACCCCCG AATCCCTCTT 
CAGAACGGTC GTCTTCCCAA TCATCTAACC CACCGACAGC ACCTCCAGAG ACTCCGAAGT 
TACAGCGCTG GAGAGGCCTC AGAAGTTTCT AGAAACAGAG GAGCCTCTTT ACTGGCCAGG 
CCAGGACACA GCTTAGTAGC TGCTATTCGA AGCCAACATC AACATGAGTC ATTGCCACTG 
GCATATAATG ACAAGATTGT GGCATTTCTT CGCCAGCCAA ACATTTTTGA AATGCTGCAA 
GAGCGTCAGC CAAGCTTAGC AAGAAACCAC ACACTCAGGG AGAAAATCCA TTACATTCGG 
ACTGAGGGTA ATCACGGGCT TGAGAAGTTG TCCTGTGATG CGGATCTGGT CATTTTGCTG 
AGTCTCTTTG AAGAAGAGAT TATGTCCTAC GTCCCCCTGC AGGCTGCCTT CCACCCTGGG 
TATAGCTTCT CTCCTCGCTG TTCACCCTGT TCTTCACCTC AGAACTCCCC AGGTTTACAG 
AGAGCCAGCG CAAGAGCCCC TTCCCCCTAC CGGAGAGACT TTGAGGCCAA GCTCCGCAAT 
TTCTACAGAA AACTGGAAGC CAAAGGATTT GGTCAGGGTC CAGGGAAAAT TAAGCTCATT 
ATTCGCCGGG ATCATTTGTT GGAGGGAACC TTCAATCAAG TGATGGCTTA TTCGCGGAAA 
GAGCTCCAGC GAAACAAGCT CTATGTCACC TTTGTTGGAG AGGAGGGCCT GGACTACAGT 
GGCCCCTCGC GGGAATTCTT CTTCCTTCTG TCTCAGGAGC TCTTCAACCC TTACTATGGA 
CTCTTTGAGT ACTCGGCAAA TGATACTTAC ACGGTGCAGA TCAGCCCCAT GTCCGCATTT 
GTAGAAAACC ATCTTGAGTG GTTCAGGTTT AGCGGTCGCA TCCTGGGCCT GGCTCTGATC 
CATCAGTACC TTCTTGACGC TTTCTTCACG AGGCCCTTCT ACAAGGCACT CCTGAGACTG 
CCCTGTGATT TGAGTGACCT GGAATATTTG GATGAGGAAT TCCACCAGAG CTTGCAGTGG 
ATGAAGGACA ACAACATCAC AGACATCCTA GACCTCACTT TCACCGTTAA TGAAGAGGTT 
TTTGGACAGG TCACGGAAAG GGAGTTGAAG TCTGGAGGAG CCAACACACA GGTGACGGAG 
AAGAACAAGA AGGAGTACAT CGAGCGCATG GTGAAGTGGC GGGTGGAACG CGGCGTGGTA 
CAGCAGACCG AGGCACTGGT GCGCGGCTTC TACGAGGTTG TAGACTCGAG GCTGGTGTCC 
GTGTTTGATG CCAGGGAGCT GGAGCTGGTG ATAGCTGGCA CCGCGGAAAT CGACCTAAAT 
GACTGGCGGA ATAACACCGA GTACCGGGGA GGTTACCACG ATGGGCATCT TGTGATCCGC 
TGGTTCTGGG CTGCAGTGGA GCGCTTCAAT AATGAGCAGA GGCTGAGATT ACTGCAGTTT 
GTCACGGGAA CATCCAGCGT GCCCTACGAA GGCTTCGCAG CCCTCCGTGG GAGCAATGGG 
CTTCGGCGCT TCTGCATAGA GAAATGGGGG AAAATTACCT CTCTCCCCAG GGCACACACA 
TGCTTCAACC GACTGGATCT TCCACCGTAT CCCTCGTACT CCATGTTGTA TGAAAAGCTG 
TTAACAGCAG TAGAGGAAAC CAGCACCTTT GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014989018.1
CDS	340..5061
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015133532.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015133532.1

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCTCTC 
CGATACAGCT ACAATCCTGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCATGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACCGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTACTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCA TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATCGGCGCTG ATGAGACTGT ACAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAGCACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACGGAGCCT 
GAGTCAGCCC CCATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGATCTG AGGCACCGGA GTCCTCTGAG GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCTGG ATGGTCCAGG GAACCAAAGC ATGGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GACCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA GCACTGCTGC TGGAAGACGG TGAAGCACCA 
GCCAGCACCG AGGAGGAGCC CTTAGAGGAG GAAGCAACGA CACAGAGCCG GGCTGGAAGG 
GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AACTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCTGAGCTGG AGACAGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACAGT GGCGGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAT CCTGGCCACT CCGGGGGCCA CTTCTCCAGC 
CTGGCCAATG GCGCCGCCCA GGATGGCGAC ACGCACCCCA GCACCGGCAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGATGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCCTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTAG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGATGATGAG 
GAGGAGGAGA ACAGCGCGTT CGAATCTGTG CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GAGGAAAGAA CCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GACTGGGAAG CTCGAATTGA CAGCCACGGG CGGGTCTTTT ATGTGGACCA CGTGAACCGC 
ACAACCACCT GGCAGCGTCC AACGGCAGCA GCCACCCCGG ATGGCATGCG GAGATCGGGG 
TCCATCCAGC AGATGGAGCA ACTCAACAGG CGGTATCAAA ACATTCAGCG AACCATTGCA 
ACAGAGAGGT CGGAAGAAGA TTCTGGCAGC CAAAGCTGCG AGCAAGCCCC AGCAGGAGGA 
GGCGGAGGTG GAGGGAGTGA CTCAGAAGCC GAATCTTCCC AGTCCAGCTT AGATCTAAGG 
AGAGAAGGGT CACTTTCTCC GGTTAACTCA CAAAAAATCA CCTTGCTGCT GCAGTCCCCA 
GCGGTCAAGT TCATCACCAA CCCCGAATTC TTCACTGTGC TGCATGCCAA TTATAGTGCC 
TACCGAGTGT TCACCAGTAG CACCTGCTTA AAGCACATGA TTCTGAAAGT CCGACGGGAT 
GCTCGCAATT TTGAACGCTA CCAGCACAAC CGGGACTTGG TGAATTTCAT CAACATGTTC 
GCAGACACTC GGCTGGAACT GCCCCGGGGC TGGGAGATCA AAACGGACCA GCAGGGAAAG 
TCTTTTTTCG TGGACCACAA CAGTCGAGCT ACCACTTTCA TTGACCCCCG AATCCCTCTT 
CAGAACGGTC GTCTTCCCAA TCATCTAACC CACCGACAGC ACCTCCAGAG ACTCCGAAGT 
TACAGCGCTG GAGAGGCCTC AGAAGTTTCT AGAAACAGAG GAGCCTCTTT ACTGGCCAGG 
CCAGGACACA GCTTAGTAGC TGCTATTCGA AGCCAACATC AACATGAGTC ATTGCCACTG 
GCATATAATG ACAAGATTGT GGCATTTCTT CGCCAGCCAA ACATTTTTGA AATGCTGCAA 
GAGCGTCAGC CAAGCTTAGC AAGAAACCAC ACACTCAGGG AGAAAATCCA TTACATTCGG 
ACTGAGGGTA ATCACGGGCT TGAGAAGTTG TCCTGTGATG CGGATCTGGT CATTTTGCTG 
AGTCTCTTTG AAGAAGAGAT TATGTCCTAC GTCCCCCTGC AGGCTGCCTT CCACCCTGGG 
TATAGCTTCT CTCCTCGCTG TTCACCCTGT TCTTCACCTC AGAACTCCCC AGGTTTACAG 
AGAGCCAGCG CAAGAGCCCC TTCCCCCTAC CGGAGAGACT TTGAGGCCAA GCTCCGCAAT 
TTCTACAGAA AACTGGAAGC CAAAGGATTT GGTCAGGGTC CAGGGAAAAT TAAGCTCATT 
ATTCGCCGGG ATCATTTGTT GGAGGGAACC TTCAATCAAG TGATGGCTTA TTCGCGGAAA 
GAGCTCCAGC GAAACAAGCT CTATGTCACC TTTGTTGGAG AGGAGGGCCT GGACTACAGT 
GGCCCCTCGC GGGAATTCTT CTTCCTTCTG TCTCAGGAGC TCTTCAACCC TTACTATGGA 
CTCTTTGAGT ACTCGGCAAA TGATACTTAC ACGGTGCAGA TCAGCCCCAT GTCCGCATTT 
GTAGAAAACC ATCTTGAGTG GTTCAGGTTT AGCGGTCGCA TCCTGGGCCT GGCTCTGATC 
CATCAGTACC TTCTTGACGC TTTCTTCACG AGGCCCTTCT ACAAGGCACT CCTGAGACTG 
CCCTGTGATT TGAGTGACCT GGAATATTTG GATGAGGAAT TCCACCAGAG CTTGCAGTGG 
ATGAAGGACA ACAACATCAC AGACATCCTA GACCTCACTT TCACCGTTAA TGAAGAGGTT 
TTTGGACAGG TCACGGAAAG GGAGTTGAAG TCTGGAGGAG CCAACACACA GGTGACGGAG 
AAGAACAAGA AGGAGTACAT CGAGCGCATG GTGAAGTGGC GGGTGGAACG CGGCGTGGTA 
CAGCAGACCG AGGCACTGGT GCGCGGCTTC TACGAGGTTG TAGACTCGAG GCTGGTGTCC 
GTGTTTGATG CCAGGGAGCT GGAGCTGGTG ATAGCTGGCA CCGCGGAAAT CGACCTAAAT 
GACTGGCGGA ATAACACCGA GTACCGGGGA GGTTACCACG ATGGGCATCT TGTGATCCGC 
TGGTTCTGGG CTGCAGTGGA GCGCTTCAAT AATGAGCAGA GGCTGAGATT ACTGCAGTTT 
GTCACGGGAA CATCCAGCGT GCCCTACGAA GGCTTCGCAG CCCTCCGTGG GAGCAATGGG 
CTTCGGCGCT TCTGCATAGA GAAATGGGGG AAAATTACCT CTCTCCCCAG GGCACACACA 
TGCTTCAACC GACTGGATCT TCCACCGTAT CCCTCGTACT CCATGTTGTA TGAAAAGCTG 
TTAACAGCAG TAGAGGAAAC CAGCACCTTT GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015133533.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014989019.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMb30257
Clone ID Related Accession (Same CDS sequence)	XM_015133533.1
Accession Version	XM_015133533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014804724.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC GGAGCCTGAG 
TCAGCCCCCA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGATCTGAGG CACCGGAGTC CTCTGAGGGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCTGGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAC 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCGA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCACCAGCC 
AGCACCGAGG AGGAGCCCTT AGAGGAGGAA GCAACGACAC AGAGCCGGGC TGGAAGGGAA 
GAAGAGAAGG AGCAGGAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAAC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCT 
GAGCTGGAGA CAGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCGGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACAGTGGC GGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCATCCT GGCCACTCCG GGGGCCACTT CTCCAGCCTG 
GCCAATGGCG CCGCCCAGGA TGGCGACACG CACCCCAGCA CCGGCAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ATGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCCTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTAGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA TGATGAGGAG 
GAGGAGAACA GCGCGTTCGA ATCTGTGCCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGAG 
GAAAGAACCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGAC 
TGGGAAGCTC GAATTGACAG CCACGGGCGG GTCTTTTATG TGGACCACGT GAACCGCACA 
ACCACCTGGC AGCGTCCAAC GGCAGCAGCC ACCCCGGATG GCATGCGGAG ATCGGGGTCC 
ATCCAGCAGA TGGAGCAACT CAACAGGCGG TATCAAAACA TTCAGCGAAC CATTGCAACA 
GAGAGGTCGG AAGAAGATTC TGGCAGCCAA AGCTGCGAGC AAGCCCCAGC AGGAGGAGGC 
GGAGGTGGAG GGAGTGACTC AGAAGCCGAA TCTTCCCAGT CCAGCTTAGA TCTAAGGAGA 
GAAGGGTCAC TTTCTCCGGT TAACTCACAA AAAATCACCT TGCTGCTGCA GTCCCCAGCG 
GTCAAGTTCA TCACCAACCC CGAATTCTTC ACTGTGCTGC ATGCCAATTA TAGTGCCTAC 
CGAGTGTTCA CCAGTAGCAC CTGCTTAAAG CACATGATTC TGAAAGTCCG ACGGGATGCT 
CGCAATTTTG AACGCTACCA GCACAACCGG GACTTGGTGA ATTTCATCAA CATGTTCGCA 
GACACTCGGC TGGAACTGCC CCGGGGCTGG GAGATCAAAA CGGACCAGCA GGGAAAGTCT 
TTTTTCGTGG ACCACAACAG TCGAGCTACC ACTTTCATTG ACCCCCGAAT CCCTCTTCAG 
AACGGTCGTC TTCCCAATCA TCTAACCCAC CGACAGCACC TCCAGAGACT CCGAAGTTAC 
AGCGCTGGAG AGGCCTCAGA AGTTTCTAGA AACAGAGGAG CCTCTTTACT GGCCAGGCCA 
GGACACAGCT TAGTAGCTGC TATTCGAAGC CAACATCAAC ATGAGTCATT GCCACTGGCA 
TATAATGACA AGATTGTGGC ATTTCTTCGC CAGCCAAACA TTTTTGAAAT GCTGCAAGAG 
CGTCAGCCAA GCTTAGCAAG AAACCACACA CTCAGGGAGA AAATCCATTA CATTCGGACT 
GAGGGTAATC ACGGGCTTGA GAAGTTGTCC TGTGATGCGG ATCTGGTCAT TTTGCTGAGT 
CTCTTTGAAG AAGAGATTAT GTCCTACGTC CCCCTGCAGG CTGCCTTCCA CCCTGGGTAT 
AGCTTCTCTC CTCGCTGTTC ACCCTGTTCT TCACCTCAGA ACTCCCCAGG TTTACAGAGA 
GCCAGCGCAA GAGCCCCTTC CCCCTACCGG AGAGACTTTG AGGCCAAGCT CCGCAATTTC 
TACAGAAAAC TGGAAGCCAA AGGATTTGGT CAGGGTCCAG GGAAAATTAA GCTCATTATT 
CGCCGGGATC ATTTGTTGGA GGGAACCTTC AATCAAGTGA TGGCTTATTC GCGGAAAGAG 
CTCCAGCGAA ACAAGCTCTA TGTCACCTTT GTTGGAGAGG AGGGCCTGGA CTACAGTGGC 
CCCTCGCGGG AATTCTTCTT CCTTCTGTCT CAGGAGCTCT TCAACCCTTA CTATGGACTC 
TTTGAGTACT CGGCAAATGA TACTTACACG GTGCAGATCA GCCCCATGTC CGCATTTGTA 
GAAAACCATC TTGAGTGGTT CAGGTTTAGC GGTCGCATCC TGGGCCTGGC TCTGATCCAT 
CAGTACCTTC TTGACGCTTT CTTCACGAGG CCCTTCTACA AGGCACTCCT GAGACTGCCC 
TGTGATTTGA GTGACCTGGA ATATTTGGAT GAGGAATTCC ACCAGAGCTT GCAGTGGATG 
AAGGACAACA ACATCACAGA CATCCTAGAC CTCACTTTCA CCGTTAATGA AGAGGTTTTT 
GGACAGGTCA CGGAAAGGGA GTTGAAGTCT GGAGGAGCCA ACACACAGGT GACGGAGAAG 
AACAAGAAGG AGTACATCGA GCGCATGGTG AAGTGGCGGG TGGAACGCGG CGTGGTACAG 
CAGACCGAGG CACTGGTGCG CGGCTTCTAC GAGGTTGTAG ACTCGAGGCT GGTGTCCGTG 
TTTGATGCCA GGGAGCTGGA GCTGGTGATA GCTGGCACCG CGGAAATCGA CCTAAATGAC 
TGGCGGAATA ACACCGAGTA CCGGGGAGGT TACCACGATG GGCATCTTGT GATCCGCTGG 
TTCTGGGCTG CAGTGGAGCG CTTCAATAAT GAGCAGAGGC TGAGATTACT GCAGTTTGTC 
ACGGGAACAT CCAGCGTGCC CTACGAAGGC TTCGCAGCCC TCCGTGGGAG CAATGGGCTT 
CGGCGCTTCT GCATAGAGAA ATGGGGGAAA ATTACCTCTC TCCCCAGGGC ACACACATGC 
TTCAACCGAC TGGATCTTCC ACCGTATCCC TCGTACTCCA TGTTGTATGA AAAGCTGTTA 
ACAGCAGTAG AGGAAACCAG CACCTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014989019.1
CDS	366..5084
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015133533.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015133533.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCTCTCCGA 
TACAGCTACA ATCCTGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCATGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTACTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCATAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATC GGCGCTGATG AGACTGTACA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA GCACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC GGAGCCTGAG 
TCAGCCCCCA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGATCTGAGG CACCGGAGTC CTCTGAGGGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCTGGATG GTCCAGGGAA CCAAAGCATG GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAC 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCGA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCGTCAGCA CTGCTGCTGG AAGACGGTGA AGCACCAGCC 
AGCACCGAGG AGGAGCCCTT AGAGGAGGAA GCAACGACAC AGAGCCGGGC TGGAAGGGAA 
GAAGAGAAGG AGCAGGAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAAC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCT 
GAGCTGGAGA CAGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCGGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACAGTGGC GGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCATCCT GGCCACTCCG GGGGCCACTT CTCCAGCCTG 
GCCAATGGCG CCGCCCAGGA TGGCGACACG CACCCCAGCA CCGGCAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ATGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCCTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTAGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA TGATGAGGAG 
GAGGAGAACA GCGCGTTCGA ATCTGTGCCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGAG 
GAAAGAACCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGAC 
TGGGAAGCTC GAATTGACAG CCACGGGCGG GTCTTTTATG TGGACCACGT GAACCGCACA 
ACCACCTGGC AGCGTCCAAC GGCAGCAGCC ACCCCGGATG GCATGCGGAG ATCGGGGTCC 
ATCCAGCAGA TGGAGCAACT CAACAGGCGG TATCAAAACA TTCAGCGAAC CATTGCAACA 
GAGAGGTCGG AAGAAGATTC TGGCAGCCAA AGCTGCGAGC AAGCCCCAGC AGGAGGAGGC 
GGAGGTGGAG GGAGTGACTC AGAAGCCGAA TCTTCCCAGT CCAGCTTAGA TCTAAGGAGA 
GAAGGGTCAC TTTCTCCGGT TAACTCACAA AAAATCACCT TGCTGCTGCA GTCCCCAGCG 
GTCAAGTTCA TCACCAACCC CGAATTCTTC ACTGTGCTGC ATGCCAATTA TAGTGCCTAC 
CGAGTGTTCA CCAGTAGCAC CTGCTTAAAG CACATGATTC TGAAAGTCCG ACGGGATGCT 
CGCAATTTTG AACGCTACCA GCACAACCGG GACTTGGTGA ATTTCATCAA CATGTTCGCA 
GACACTCGGC TGGAACTGCC CCGGGGCTGG GAGATCAAAA CGGACCAGCA GGGAAAGTCT 
TTTTTCGTGG ACCACAACAG TCGAGCTACC ACTTTCATTG ACCCCCGAAT CCCTCTTCAG 
AACGGTCGTC TTCCCAATCA TCTAACCCAC CGACAGCACC TCCAGAGACT CCGAAGTTAC 
AGCGCTGGAG AGGCCTCAGA AGTTTCTAGA AACAGAGGAG CCTCTTTACT GGCCAGGCCA 
GGACACAGCT TAGTAGCTGC TATTCGAAGC CAACATCAAC ATGAGTCATT GCCACTGGCA 
TATAATGACA AGATTGTGGC ATTTCTTCGC CAGCCAAACA TTTTTGAAAT GCTGCAAGAG 
CGTCAGCCAA GCTTAGCAAG AAACCACACA CTCAGGGAGA AAATCCATTA CATTCGGACT 
GAGGGTAATC ACGGGCTTGA GAAGTTGTCC TGTGATGCGG ATCTGGTCAT TTTGCTGAGT 
CTCTTTGAAG AAGAGATTAT GTCCTACGTC CCCCTGCAGG CTGCCTTCCA CCCTGGGTAT 
AGCTTCTCTC CTCGCTGTTC ACCCTGTTCT TCACCTCAGA ACTCCCCAGG TTTACAGAGA 
GCCAGCGCAA GAGCCCCTTC CCCCTACCGG AGAGACTTTG AGGCCAAGCT CCGCAATTTC 
TACAGAAAAC TGGAAGCCAA AGGATTTGGT CAGGGTCCAG GGAAAATTAA GCTCATTATT 
CGCCGGGATC ATTTGTTGGA GGGAACCTTC AATCAAGTGA TGGCTTATTC GCGGAAAGAG 
CTCCAGCGAA ACAAGCTCTA TGTCACCTTT GTTGGAGAGG AGGGCCTGGA CTACAGTGGC 
CCCTCGCGGG AATTCTTCTT CCTTCTGTCT CAGGAGCTCT TCAACCCTTA CTATGGACTC 
TTTGAGTACT CGGCAAATGA TACTTACACG GTGCAGATCA GCCCCATGTC CGCATTTGTA 
GAAAACCATC TTGAGTGGTT CAGGTTTAGC GGTCGCATCC TGGGCCTGGC TCTGATCCAT 
CAGTACCTTC TTGACGCTTT CTTCACGAGG CCCTTCTACA AGGCACTCCT GAGACTGCCC 
TGTGATTTGA GTGACCTGGA ATATTTGGAT GAGGAATTCC ACCAGAGCTT GCAGTGGATG 
AAGGACAACA ACATCACAGA CATCCTAGAC CTCACTTTCA CCGTTAATGA AGAGGTTTTT 
GGACAGGTCA CGGAAAGGGA GTTGAAGTCT GGAGGAGCCA ACACACAGGT GACGGAGAAG 
AACAAGAAGG AGTACATCGA GCGCATGGTG AAGTGGCGGG TGGAACGCGG CGTGGTACAG 
CAGACCGAGG CACTGGTGCG CGGCTTCTAC GAGGTTGTAG ACTCGAGGCT GGTGTCCGTG 
TTTGATGCCA GGGAGCTGGA GCTGGTGATA GCTGGCACCG CGGAAATCGA CCTAAATGAC 
TGGCGGAATA ACACCGAGTA CCGGGGAGGT TACCACGATG GGCATCTTGT GATCCGCTGG 
TTCTGGGCTG CAGTGGAGCG CTTCAATAAT GAGCAGAGGC TGAGATTACT GCAGTTTGTC 
ACGGGAACAT CCAGCGTGCC CTACGAAGGC TTCGCAGCCC TCCGTGGGAG CAATGGGCTT 
CGGCGCTTCT GCATAGAGAA ATGGGGGAAA ATTACCTCTC TCCCCAGGGC ACACACATGC 
TTCAACCGAC TGGATCTTCC ACCGTATCCC TCGTACTCCA TGTTGTATGA AAAGCTGTTA 
ACAGCAGTAG AGGAAACCAG CACCTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015133534.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014989020.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.

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CloneID	OMb30258
Clone ID Related Accession (Same CDS sequence)	XM_015133534.1 , XM_015133534.2
Accession Version	XM_015133534.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014804724.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC TGGCAGAACC CAAAGATGAC TGCATTTCCA AGGAGATTTT TAAAAGCATC 
GGCGCTGATG AGACTGTACA AGGACAAGGA AGTCGGAGGC TGATCAGCTT CTCTCTCTCA 
GATTTCCAAG CCATGGGGTT GAAGAAAGGG ATGTTTTTCA ACCCAGACCC TTATCTGAAG 
ATTTCCATTC AGCCTGGGAA GCACAGCATC TTCCCCGCCC TCCCTCACCA TGGACAGGAG 
AGGAGATCCA AGATCATAGG CAACACCGTG AACCCCATCT GGCAGGCCGA GCAATTCAGT 
TTTGTGTCCT TGCCCACTGA CGTGCTGGAA ATTGAGGTGA AGGACAAGTT TGCCAAGAGC 
CGTCCCATCA TCAAGCGTTT CTTGGGAAAG CTGTCGATGC CCGTTCAAAG ACTCCTGGAG 
AGACACGCCA TAGGGGATAG GGTGGTCAGC TACACACTTG GCCGCAGGCT TCCAACAGAT 
CATGTGAGTG GACAGCTGCA ATTCCGATTT GAGATCACTT CCTCCATCCA CCCAGCAGAT 
GATGAGGAGA TTTCCCTGAG TACGGAGCCT GAGTCAGCCC CCATTCAGGA CAGCCCCATG 
AACAACCTGA TGGAAAGCGG CAGTGGGGAA CCTCGATCTG AGGCACCGGA GTCCTCTGAG 
GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC AGTGTCCTGG ATGGTCCAGG GAACCAAAGC 
ATGGAGCTTT CCAGACCAGC TGAGGAAGCA GCAGTCATCA CGGAGGCAGG AGACCAGGGC 
ATGGTCTCTG TGGGACCTGA AGGGGCTGGG GACCTCCTGG CCCAGGTGCA AAAGGACATC 
CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA 
GCACTGCTGC TGGAAGACGG TGAAGCACCA GCCAGCACCG AGGAGGAGCC CTTAGAGGAG 
GAAGCAACGA CACAGAGCCG GGCTGGAAGG GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG 
GGAGATGTGT CTACCCTGGA GCAGGGAGAG GGCAGGCTGC AACTGCGGGC CTCGGTGAAG 
AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG TCTGAGCTGG AGACAGTGAT CGCGTCAGCC 
TGCGGGGACC CCGAGACCCC GCGGACACAC TACATCCGCA TCCACACCCT GCTGCACAGC 
ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC 
ACCCTCAAGG ACTCCTCGGA GAAGGATGGG CTCAGCGAGG TGGACACAGT GGCGGCTGAC 
CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG CCCGAGGGGG CTACTCCAGG CACGGCGCAT 
CCTGGCCACT CCGGGGGCCA CTTCTCCAGC CTGGCCAATG GCGCCGCCCA GGATGGCGAC 
ACGCACCCCA GCACCGGCAG CGAGAGCGAC TCCAGCCCCA GGCAAGGCGG GGACCACAGT 
TGCGAGGGCT GTGATGCGTC CTGCTGCAGC CCCTCGTGCT ACAGCTCCTC GTGCTACAGC 
ACGTCCTGCT ACAGCAGCTC GTGCTACAGC GCCTCCTGCT ACAGCCCCTC CTGCTACAAC 
GGCAACAGGT TCGCCAGCCA CACGCGCTTC TCCTCCGTAG ACAGCGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA AGATGATGAG GAGGAGGAGA ACAGCGCGTT CGAATCTGTG 
CCCGACTCCA TGCAGAGCCC TGAGCTGGAC CCGGAGTCCA CGAACGGCGC TGGGCCGTGG 
CAAGACGAGC TGGCCGCCCC TAGCGGGCAC GAGGAAAGAA CCCCGGAAGG TCTGGAATCC 
CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAACTGGGA AGCTCGAATT GACAGCCACG GGCGGGTCTT TTATGTGGAC 
CACGTGAACC GCACAACCAC CTGGCAGCGT CCAACGGCAG CAGCCACCCC GGATGGCATG 
CGGAGATCGG GGTCCATCCA GCAGATGGAG CAACTCAACA GGCGGTATCA AAACATTCAG 
CGAACCATTG CAACAGAGAG GTCGGAAGAA GATTCTGGCA GCCAAAGCTG CGAGCAAGCC 
CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT GACTCAGAAG CCGAATCTTC CCAGTCCAGC 
TTAGATCTAA GGAGAGAAGG GTCACTTTCT CCGGTTAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCCC CAGCGGTCAA GTTCATCACC AACCCCGAAT TCTTCACTGT GCTGCATGCC 
AATTATAGTG CCTACCGAGT GTTCACCAGT AGCACCTGCT TAAAGCACAT GATTCTGAAA 
GTCCGACGGG ATGCTCGCAA TTTTGAACGC TACCAGCACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TCGCAGACAC TCGGCTGGAA CTGCCCCGGG GCTGGGAGAT CAAAACGGAC 
CAGCAGGGAA AGTCTTTTTT CGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAACGG TCGTCTTCCC AATCATCTAA CCCACCGACA GCACCTCCAG 
AGACTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTTT CTAGAAACAG AGGAGCCTCT 
TTACTGGCCA GGCCAGGACA CAGCTTAGTA GCTGCTATTC GAAGCCAACA TCAACATGAG 
TCATTGCCAC TGGCATATAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTGC AAGAGCGTCA GCCAAGCTTA GCAAGAAACC ACACACTCAG GGAGAAAATC 
CATTACATTC GGACTGAGGG TAATCACGGG CTTGAGAAGT TGTCCTGTGA TGCGGATCTG 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATTATGTCCT ACGTCCCCCT GCAGGCTGCC 
TTCCACCCTG GGTATAGCTT CTCTCCTCGC TGTTCACCCT GTTCTTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTACAG AAAACTGGAA GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA 
ATTAAGCTCA TTATTCGCCG GGATCATTTG TTGGAGGGAA CCTTCAATCA AGTGATGGCT 
TATTCGCGGA AAGAGCTCCA GCGAAACAAG CTCTATGTCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGCCCCTC GCGGGAATTC TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC 
CCTTACTATG GACTCTTTGA GTACTCGGCA AATGATACTT ACACGGTGCA GATCAGCCCC 
ATGTCCGCAT TTGTAGAAAA CCATCTTGAG TGGTTCAGGT TTAGCGGTCG CATCCTGGGC 
CTGGCTCTGA TCCATCAGTA CCTTCTTGAC GCTTTCTTCA CGAGGCCCTT CTACAAGGCA 
CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC CTGGAATATT TGGATGAGGA ATTCCACCAG 
AGCTTGCAGT GGATGAAGGA CAACAACATC ACAGACATCC TAGACCTCAC TTTCACCGTT 
AATGAAGAGG TTTTTGGACA GGTCACGGAA AGGGAGTTGA AGTCTGGAGG AGCCAACACA 
CAGGTGACGG AGAAGAACAA GAAGGAGTAC ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA 
CGCGGCGTGG TACAGCAGAC CGAGGCACTG GTGCGCGGCT TCTACGAGGT TGTAGACTCG 
AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG CTGGAGCTGG TGATAGCTGG CACCGCGGAA 
ATCGACCTAA ATGACTGGCG GAATAACACC GAGTACCGGG GAGGTTACCA CGATGGGCAT 
CTTGTGATCC GCTGGTTCTG GGCTGCAGTG GAGCGCTTCA ATAATGAGCA GAGGCTGAGA 
TTACTGCAGT TTGTCACGGG AACATCCAGC GTGCCCTACG AAGGCTTCGC AGCCCTCCGT 
GGGAGCAATG GGCTTCGGCG CTTCTGCATA GAGAAATGGG GGAAAATTAC CTCTCTCCCC 
AGGGCACACA CATGCTTCAA CCGACTGGAT CTTCCACCGT ATCCCTCGTA CTCCATGTTG 
TATGAAAAGC TGTTAACAGC AGTAGAGGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014989020.1
CDS	144..4457
Translation	MAALAEPKDDCISKEIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015133534.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015133534.1

ORF Insert Sequence:

ATGGCTGCTC TGGCAGAACC CAAAGATGAC TGCATTTCCA AGGAGATTTT TAAAAGCATC 
GGCGCTGATG AGACTGTACA AGGACAAGGA AGTCGGAGGC TGATCAGCTT CTCTCTCTCA 
GATTTCCAAG CCATGGGGTT GAAGAAAGGG ATGTTTTTCA ACCCAGACCC TTATCTGAAG 
ATTTCCATTC AGCCTGGGAA GCACAGCATC TTCCCCGCCC TCCCTCACCA TGGACAGGAG 
AGGAGATCCA AGATCATAGG CAACACCGTG AACCCCATCT GGCAGGCCGA GCAATTCAGT 
TTTGTGTCCT TGCCCACTGA CGTGCTGGAA ATTGAGGTGA AGGACAAGTT TGCCAAGAGC 
CGTCCCATCA TCAAGCGTTT CTTGGGAAAG CTGTCGATGC CCGTTCAAAG ACTCCTGGAG 
AGACACGCCA TAGGGGATAG GGTGGTCAGC TACACACTTG GCCGCAGGCT TCCAACAGAT 
CATGTGAGTG GACAGCTGCA ATTCCGATTT GAGATCACTT CCTCCATCCA CCCAGCAGAT 
GATGAGGAGA TTTCCCTGAG TACGGAGCCT GAGTCAGCCC CCATTCAGGA CAGCCCCATG 
AACAACCTGA TGGAAAGCGG CAGTGGGGAA CCTCGATCTG AGGCACCGGA GTCCTCTGAG 
GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC AGTGTCCTGG ATGGTCCAGG GAACCAAAGC 
ATGGAGCTTT CCAGACCAGC TGAGGAAGCA GCAGTCATCA CGGAGGCAGG AGACCAGGGC 
ATGGTCTCTG TGGGACCTGA AGGGGCTGGG GACCTCCTGG CCCAGGTGCA AAAGGACATC 
CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA 
GCACTGCTGC TGGAAGACGG TGAAGCACCA GCCAGCACCG AGGAGGAGCC CTTAGAGGAG 
GAAGCAACGA CACAGAGCCG GGCTGGAAGG GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG 
GGAGATGTGT CTACCCTGGA GCAGGGAGAG GGCAGGCTGC AACTGCGGGC CTCGGTGAAG 
AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG TCTGAGCTGG AGACAGTGAT CGCGTCAGCC 
TGCGGGGACC CCGAGACCCC GCGGACACAC TACATCCGCA TCCACACCCT GCTGCACAGC 
ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC 
ACCCTCAAGG ACTCCTCGGA GAAGGATGGG CTCAGCGAGG TGGACACAGT GGCGGCTGAC 
CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG CCCGAGGGGG CTACTCCAGG CACGGCGCAT 
CCTGGCCACT CCGGGGGCCA CTTCTCCAGC CTGGCCAATG GCGCCGCCCA GGATGGCGAC 
ACGCACCCCA GCACCGGCAG CGAGAGCGAC TCCAGCCCCA GGCAAGGCGG GGACCACAGT 
TGCGAGGGCT GTGATGCGTC CTGCTGCAGC CCCTCGTGCT ACAGCTCCTC GTGCTACAGC 
ACGTCCTGCT ACAGCAGCTC GTGCTACAGC GCCTCCTGCT ACAGCCCCTC CTGCTACAAC 
GGCAACAGGT TCGCCAGCCA CACGCGCTTC TCCTCCGTAG ACAGCGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA AGATGATGAG GAGGAGGAGA ACAGCGCGTT CGAATCTGTG 
CCCGACTCCA TGCAGAGCCC TGAGCTGGAC CCGGAGTCCA CGAACGGCGC TGGGCCGTGG 
CAAGACGAGC TGGCCGCCCC TAGCGGGCAC GAGGAAAGAA CCCCGGAAGG TCTGGAATCC 
CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAACTGGGA AGCTCGAATT GACAGCCACG GGCGGGTCTT TTATGTGGAC 
CACGTGAACC GCACAACCAC CTGGCAGCGT CCAACGGCAG CAGCCACCCC GGATGGCATG 
CGGAGATCGG GGTCCATCCA GCAGATGGAG CAACTCAACA GGCGGTATCA AAACATTCAG 
CGAACCATTG CAACAGAGAG GTCGGAAGAA GATTCTGGCA GCCAAAGCTG CGAGCAAGCC 
CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT GACTCAGAAG CCGAATCTTC CCAGTCCAGC 
TTAGATCTAA GGAGAGAAGG GTCACTTTCT CCGGTTAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCCC CAGCGGTCAA GTTCATCACC AACCCCGAAT TCTTCACTGT GCTGCATGCC 
AATTATAGTG CCTACCGAGT GTTCACCAGT AGCACCTGCT TAAAGCACAT GATTCTGAAA 
GTCCGACGGG ATGCTCGCAA TTTTGAACGC TACCAGCACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TCGCAGACAC TCGGCTGGAA CTGCCCCGGG GCTGGGAGAT CAAAACGGAC 
CAGCAGGGAA AGTCTTTTTT CGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAACGG TCGTCTTCCC AATCATCTAA CCCACCGACA GCACCTCCAG 
AGACTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTTT CTAGAAACAG AGGAGCCTCT 
TTACTGGCCA GGCCAGGACA CAGCTTAGTA GCTGCTATTC GAAGCCAACA TCAACATGAG 
TCATTGCCAC TGGCATATAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTGC AAGAGCGTCA GCCAAGCTTA GCAAGAAACC ACACACTCAG GGAGAAAATC 
CATTACATTC GGACTGAGGG TAATCACGGG CTTGAGAAGT TGTCCTGTGA TGCGGATCTG 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATTATGTCCT ACGTCCCCCT GCAGGCTGCC 
TTCCACCCTG GGTATAGCTT CTCTCCTCGC TGTTCACCCT GTTCTTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTACAG AAAACTGGAA GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA 
ATTAAGCTCA TTATTCGCCG GGATCATTTG TTGGAGGGAA CCTTCAATCA AGTGATGGCT 
TATTCGCGGA AAGAGCTCCA GCGAAACAAG CTCTATGTCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGCCCCTC GCGGGAATTC TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC 
CCTTACTATG GACTCTTTGA GTACTCGGCA AATGATACTT ACACGGTGCA GATCAGCCCC 
ATGTCCGCAT TTGTAGAAAA CCATCTTGAG TGGTTCAGGT TTAGCGGTCG CATCCTGGGC 
CTGGCTCTGA TCCATCAGTA CCTTCTTGAC GCTTTCTTCA CGAGGCCCTT CTACAAGGCA 
CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC CTGGAATATT TGGATGAGGA ATTCCACCAG 
AGCTTGCAGT GGATGAAGGA CAACAACATC ACAGACATCC TAGACCTCAC TTTCACCGTT 
AATGAAGAGG TTTTTGGACA GGTCACGGAA AGGGAGTTGA AGTCTGGAGG AGCCAACACA 
CAGGTGACGG AGAAGAACAA GAAGGAGTAC ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA 
CGCGGCGTGG TACAGCAGAC CGAGGCACTG GTGCGCGGCT TCTACGAGGT TGTAGACTCG 
AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG CTGGAGCTGG TGATAGCTGG CACCGCGGAA 
ATCGACCTAA ATGACTGGCG GAATAACACC GAGTACCGGG GAGGTTACCA CGATGGGCAT 
CTTGTGATCC GCTGGTTCTG GGCTGCAGTG GAGCGCTTCA ATAATGAGCA GAGGCTGAGA 
TTACTGCAGT TTGTCACGGG AACATCCAGC GTGCCCTACG AAGGCTTCGC AGCCCTCCGT 
GGGAGCAATG GGCTTCGGCG CTTCTGCATA GAGAAATGGG GGAAAATTAC CTCTCTCCCC 
AGGGCACACA CATGCTTCAA CCGACTGGAT CTTCCACCGT ATCCCTCGTA CTCCATGTTG 
TATGAAAAGC TGTTAACAGC AGTAGAGGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_015133534.2 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014989020.1 Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X11, mRNA.

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CloneID	OMb30258
Clone ID Related Accession (Same CDS sequence)	XM_015133534.1 , XM_015133534.2
Accession Version	XM_015133534.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	E3 ubiquitin-protein ligase HECW1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041756.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2019 this sequence version replaced XM_015133534.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC TGGCAGAACC CAAAGATGAC TGCATTTCCA AGGAGATTTT TAAAAGCATC 
GGCGCTGATG AGACTGTACA AGGACAAGGA AGTCGGAGGC TGATCAGCTT CTCTCTCTCA 
GATTTCCAAG CCATGGGGTT GAAGAAAGGG ATGTTTTTCA ACCCAGACCC TTATCTGAAG 
ATTTCCATTC AGCCTGGGAA GCACAGCATC TTCCCCGCCC TCCCTCACCA TGGACAGGAG 
AGGAGATCCA AGATCATAGG CAACACCGTG AACCCCATCT GGCAGGCCGA GCAATTCAGT 
TTTGTGTCCT TGCCCACTGA CGTGCTGGAA ATTGAGGTGA AGGACAAGTT TGCCAAGAGC 
CGTCCCATCA TCAAGCGTTT CTTGGGAAAG CTGTCGATGC CCGTTCAAAG ACTCCTGGAG 
AGACACGCCA TAGGGGATAG GGTGGTCAGC TACACACTTG GCCGCAGGCT TCCAACAGAT 
CATGTGAGTG GACAGCTGCA ATTCCGATTT GAGATCACTT CCTCCATCCA CCCAGCAGAT 
GATGAGGAGA TTTCCCTGAG TACGGAGCCT GAGTCAGCCC CCATTCAGGA CAGCCCCATG 
AACAACCTGA TGGAAAGCGG CAGTGGGGAA CCTCGATCTG AGGCACCGGA GTCCTCTGAG 
GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC AGTGTCCTGG ATGGTCCAGG GAACCAAAGC 
ATGGAGCTTT CCAGACCAGC TGAGGAAGCA GCAGTCATCA CGGAGGCAGG AGACCAGGGC 
ATGGTCTCTG TGGGACCTGA AGGGGCTGGG GACCTCCTGG CCCAGGTGCA AAAGGACATC 
CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA 
GCACTGCTGC TGGAAGACGG TGAAGCACCA GCCAGCACCG AGGAGGAGCC CTTAGAGGAG 
GAAGCAACGA CACAGAGCCG GGCTGGAAGG GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG 
GGAGATGTGT CTACCCTGGA GCAGGGAGAG GGCAGGCTGC AACTGCGGGC CTCGGTGAAG 
AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG TCTGAGCTGG AGACAGTGAT CGCGTCAGCC 
TGCGGGGACC CCGAGACCCC GCGGACACAC TACATCCGCA TCCACACCCT GCTGCACAGC 
ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC 
ACCCTCAAGG ACTCCTCGGA GAAGGATGGG CTCAGCGAGG TGGACACAGT GGCGGCTGAC 
CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG CCCGAGGGGG CTACTCCAGG CACGGCGCAT 
CCTGGCCACT CCGGGGGCCA CTTCTCCAGC CTGGCCAATG GCGCCGCCCA GGATGGCGAC 
ACGCACCCCA GCACCGGCAG CGAGAGCGAC TCCAGCCCCA GGCAAGGCGG GGACCACAGT 
TGCGAGGGCT GTGATGCGTC CTGCTGCAGC CCCTCGTGCT ACAGCTCCTC GTGCTACAGC 
ACGTCCTGCT ACAGCAGCTC GTGCTACAGC GCCTCCTGCT ACAGCCCCTC CTGCTACAAC 
GGCAACAGGT TCGCCAGCCA CACGCGCTTC TCCTCCGTAG ACAGCGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA AGATGATGAG GAGGAGGAGA ACAGCGCGTT CGAATCTGTG 
CCCGACTCCA TGCAGAGCCC TGAGCTGGAC CCGGAGTCCA CGAACGGCGC TGGGCCGTGG 
CAAGACGAGC TGGCCGCCCC TAGCGGGCAC GAGGAAAGAA CCCCGGAAGG TCTGGAATCC 
CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAACTGGGA AGCTCGAATT GACAGCCACG GGCGGGTCTT TTATGTGGAC 
CACGTGAACC GCACAACCAC CTGGCAGCGT CCAACGGCAG CAGCCACCCC GGATGGCATG 
CGGAGATCGG GGTCCATCCA GCAGATGGAG CAACTCAACA GGCGGTATCA AAACATTCAG 
CGAACCATTG CAACAGAGAG GTCGGAAGAA GATTCTGGCA GCCAAAGCTG CGAGCAAGCC 
CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT GACTCAGAAG CCGAATCTTC CCAGTCCAGC 
TTAGATCTAA GGAGAGAAGG GTCACTTTCT CCGGTTAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCCC CAGCGGTCAA GTTCATCACC AACCCCGAAT TCTTCACTGT GCTGCATGCC 
AATTATAGTG CCTACCGAGT GTTCACCAGT AGCACCTGCT TAAAGCACAT GATTCTGAAA 
GTCCGACGGG ATGCTCGCAA TTTTGAACGC TACCAGCACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TCGCAGACAC TCGGCTGGAA CTGCCCCGGG GCTGGGAGAT CAAAACGGAC 
CAGCAGGGAA AGTCTTTTTT CGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAACGG TCGTCTTCCC AATCATCTAA CCCACCGACA GCACCTCCAG 
AGACTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTTT CTAGAAACAG AGGAGCCTCT 
TTACTGGCCA GGCCAGGACA CAGCTTAGTA GCTGCTATTC GAAGCCAACA TCAACATGAG 
TCATTGCCAC TGGCATATAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTGC AAGAGCGTCA GCCAAGCTTA GCAAGAAACC ACACACTCAG GGAGAAAATC 
CATTACATTC GGACTGAGGG TAATCACGGG CTTGAGAAGT TGTCCTGTGA TGCGGATCTG 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATTATGTCCT ACGTCCCCCT GCAGGCTGCC 
TTCCACCCTG GGTATAGCTT CTCTCCTCGC TGTTCACCCT GTTCTTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTACAG AAAACTGGAA GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA 
ATTAAGCTCA TTATTCGCCG GGATCATTTG TTGGAGGGAA CCTTCAATCA AGTGATGGCT 
TATTCGCGGA AAGAGCTCCA GCGAAACAAG CTCTATGTCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGCCCCTC GCGGGAATTC TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC 
CCTTACTATG GACTCTTTGA GTACTCGGCA AATGATACTT ACACGGTGCA GATCAGCCCC 
ATGTCCGCAT TTGTAGAAAA CCATCTTGAG TGGTTCAGGT TTAGCGGTCG CATCCTGGGC 
CTGGCTCTGA TCCATCAGTA CCTTCTTGAC GCTTTCTTCA CGAGGCCCTT CTACAAGGCA 
CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC CTGGAATATT TGGATGAGGA ATTCCACCAG 
AGCTTGCAGT GGATGAAGGA CAACAACATC ACAGACATCC TAGACCTCAC TTTCACCGTT 
AATGAAGAGG TTTTTGGACA GGTCACGGAA AGGGAGTTGA AGTCTGGAGG AGCCAACACA 
CAGGTGACGG AGAAGAACAA GAAGGAGTAC ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA 
CGCGGCGTGG TACAGCAGAC CGAGGCACTG GTGCGCGGCT TCTACGAGGT TGTAGACTCG 
AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG CTGGAGCTGG TGATAGCTGG CACCGCGGAA 
ATCGACCTAA ATGACTGGCG GAATAACACC GAGTACCGGG GAGGTTACCA CGATGGGCAT 
CTTGTGATCC GCTGGTTCTG GGCTGCAGTG GAGCGCTTCA ATAATGAGCA GAGGCTGAGA 
TTACTGCAGT TTGTCACGGG AACATCCAGC GTGCCCTACG AAGGCTTCGC AGCCCTCCGT 
GGGAGCAATG GGCTTCGGCG CTTCTGCATA GAGAAATGGG GGAAAATTAC CTCTCTCCCC 
AGGGCACACA CATGCTTCAA CCGACTGGAT CTTCCACCGT ATCCCTCGTA CTCCATGTTG 
TATGAAAAGC TGTTAACAGC AGTAGAGGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014989020.1
CDS	207..4520
Translation	MAALAEPKDDCISKEIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAPIQDSPMNNLMESGSGEPRSEAPESSEGWKPEQLGEGSVLDGPGNQSMELSRPAEEAAVITEAGDQGMVSVGPEGAGDLLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTEEEPLEEEATTQSRAGREEEKEQEEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFSSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHEERTPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015133534.2
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015133534.2

ORF Insert Sequence:

ATGGCTGCTC TGGCAGAACC CAAAGATGAC TGCATTTCCA AGGAGATTTT TAAAAGCATC 
GGCGCTGATG AGACTGTACA AGGACAAGGA AGTCGGAGGC TGATCAGCTT CTCTCTCTCA 
GATTTCCAAG CCATGGGGTT GAAGAAAGGG ATGTTTTTCA ACCCAGACCC TTATCTGAAG 
ATTTCCATTC AGCCTGGGAA GCACAGCATC TTCCCCGCCC TCCCTCACCA TGGACAGGAG 
AGGAGATCCA AGATCATAGG CAACACCGTG AACCCCATCT GGCAGGCCGA GCAATTCAGT 
TTTGTGTCCT TGCCCACTGA CGTGCTGGAA ATTGAGGTGA AGGACAAGTT TGCCAAGAGC 
CGTCCCATCA TCAAGCGTTT CTTGGGAAAG CTGTCGATGC CCGTTCAAAG ACTCCTGGAG 
AGACACGCCA TAGGGGATAG GGTGGTCAGC TACACACTTG GCCGCAGGCT TCCAACAGAT 
CATGTGAGTG GACAGCTGCA ATTCCGATTT GAGATCACTT CCTCCATCCA CCCAGCAGAT 
GATGAGGAGA TTTCCCTGAG TACGGAGCCT GAGTCAGCCC CCATTCAGGA CAGCCCCATG 
AACAACCTGA TGGAAAGCGG CAGTGGGGAA CCTCGATCTG AGGCACCGGA GTCCTCTGAG 
GGCTGGAAGC CAGAGCAGCT GGGTGAGGGC AGTGTCCTGG ATGGTCCAGG GAACCAAAGC 
ATGGAGCTTT CCAGACCAGC TGAGGAAGCA GCAGTCATCA CGGAGGCAGG AGACCAGGGC 
ATGGTCTCTG TGGGACCTGA AGGGGCTGGG GACCTCCTGG CCCAGGTGCA AAAGGACATC 
CAGCCTGCCC CGAGTGCAGA AGAGCTGGCC GAGCAGCTGG ACCTGGGTGA GGAGGCGTCA 
GCACTGCTGC TGGAAGACGG TGAAGCACCA GCCAGCACCG AGGAGGAGCC CTTAGAGGAG 
GAAGCAACGA CACAGAGCCG GGCTGGAAGG GAAGAAGAGA AGGAGCAGGA GGAGGAGGAG 
GGAGATGTGT CTACCCTGGA GCAGGGAGAG GGCAGGCTGC AACTGCGGGC CTCGGTGAAG 
AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG TCTGAGCTGG AGACAGTGAT CGCGTCAGCC 
TGCGGGGACC CCGAGACCCC GCGGACACAC TACATCCGCA TCCACACCCT GCTGCACAGC 
ATGCCCTCGG CCCAGGGCGG CAGCGCGGCA GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC 
ACCCTCAAGG ACTCCTCGGA GAAGGATGGG CTCAGCGAGG TGGACACAGT GGCGGCTGAC 
CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG CCCGAGGGGG CTACTCCAGG CACGGCGCAT 
CCTGGCCACT CCGGGGGCCA CTTCTCCAGC CTGGCCAATG GCGCCGCCCA GGATGGCGAC 
ACGCACCCCA GCACCGGCAG CGAGAGCGAC TCCAGCCCCA GGCAAGGCGG GGACCACAGT 
TGCGAGGGCT GTGATGCGTC CTGCTGCAGC CCCTCGTGCT ACAGCTCCTC GTGCTACAGC 
ACGTCCTGCT ACAGCAGCTC GTGCTACAGC GCCTCCTGCT ACAGCCCCTC CTGCTACAAC 
GGCAACAGGT TCGCCAGCCA CACGCGCTTC TCCTCCGTAG ACAGCGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA AGATGATGAG GAGGAGGAGA ACAGCGCGTT CGAATCTGTG 
CCCGACTCCA TGCAGAGCCC TGAGCTGGAC CCGGAGTCCA CGAACGGCGC TGGGCCGTGG 
CAAGACGAGC TGGCCGCCCC TAGCGGGCAC GAGGAAAGAA CCCCGGAAGG TCTGGAATCC 
CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAACTGGGA AGCTCGAATT GACAGCCACG GGCGGGTCTT TTATGTGGAC 
CACGTGAACC GCACAACCAC CTGGCAGCGT CCAACGGCAG CAGCCACCCC GGATGGCATG 
CGGAGATCGG GGTCCATCCA GCAGATGGAG CAACTCAACA GGCGGTATCA AAACATTCAG 
CGAACCATTG CAACAGAGAG GTCGGAAGAA GATTCTGGCA GCCAAAGCTG CGAGCAAGCC 
CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT GACTCAGAAG CCGAATCTTC CCAGTCCAGC 
TTAGATCTAA GGAGAGAAGG GTCACTTTCT CCGGTTAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCCC CAGCGGTCAA GTTCATCACC AACCCCGAAT TCTTCACTGT GCTGCATGCC 
AATTATAGTG CCTACCGAGT GTTCACCAGT AGCACCTGCT TAAAGCACAT GATTCTGAAA 
GTCCGACGGG ATGCTCGCAA TTTTGAACGC TACCAGCACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TCGCAGACAC TCGGCTGGAA CTGCCCCGGG GCTGGGAGAT CAAAACGGAC 
CAGCAGGGAA AGTCTTTTTT CGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAACGG TCGTCTTCCC AATCATCTAA CCCACCGACA GCACCTCCAG 
AGACTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTTT CTAGAAACAG AGGAGCCTCT 
TTACTGGCCA GGCCAGGACA CAGCTTAGTA GCTGCTATTC GAAGCCAACA TCAACATGAG 
TCATTGCCAC TGGCATATAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTGC AAGAGCGTCA GCCAAGCTTA GCAAGAAACC ACACACTCAG GGAGAAAATC 
CATTACATTC GGACTGAGGG TAATCACGGG CTTGAGAAGT TGTCCTGTGA TGCGGATCTG 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATTATGTCCT ACGTCCCCCT GCAGGCTGCC 
TTCCACCCTG GGTATAGCTT CTCTCCTCGC TGTTCACCCT GTTCTTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG CGCAAGAGCC CCTTCCCCCT ACCGGAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTACAG AAAACTGGAA GCCAAAGGAT TTGGTCAGGG TCCAGGGAAA 
ATTAAGCTCA TTATTCGCCG GGATCATTTG TTGGAGGGAA CCTTCAATCA AGTGATGGCT 
TATTCGCGGA AAGAGCTCCA GCGAAACAAG CTCTATGTCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGCCCCTC GCGGGAATTC TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC 
CCTTACTATG GACTCTTTGA GTACTCGGCA AATGATACTT ACACGGTGCA GATCAGCCCC 
ATGTCCGCAT TTGTAGAAAA CCATCTTGAG TGGTTCAGGT TTAGCGGTCG CATCCTGGGC 
CTGGCTCTGA TCCATCAGTA CCTTCTTGAC GCTTTCTTCA CGAGGCCCTT CTACAAGGCA 
CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC CTGGAATATT TGGATGAGGA ATTCCACCAG 
AGCTTGCAGT GGATGAAGGA CAACAACATC ACAGACATCC TAGACCTCAC TTTCACCGTT 
AATGAAGAGG TTTTTGGACA GGTCACGGAA AGGGAGTTGA AGTCTGGAGG AGCCAACACA 
CAGGTGACGG AGAAGAACAA GAAGGAGTAC ATCGAGCGCA TGGTGAAGTG GCGGGTGGAA 
CGCGGCGTGG TACAGCAGAC CGAGGCACTG GTGCGCGGCT TCTACGAGGT TGTAGACTCG 
AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG CTGGAGCTGG TGATAGCTGG CACCGCGGAA 
ATCGACCTAA ATGACTGGCG GAATAACACC GAGTACCGGG GAGGTTACCA CGATGGGCAT 
CTTGTGATCC GCTGGTTCTG GGCTGCAGTG GAGCGCTTCA ATAATGAGCA GAGGCTGAGA 
TTACTGCAGT TTGTCACGGG AACATCCAGC GTGCCCTACG AAGGCTTCGC AGCCCTCCGT 
GGGAGCAATG GGCTTCGGCG CTTCTGCATA GAGAAATGGG GGAAAATTAC CTCTCTCCCC 
AGGGCACACA CATGCTTCAA CCGACTGGAT CTTCCACCGT ATCCCTCGTA CTCCATGTTG 
TATGAAAAGC TGTTAACAGC AGTAGAGGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	HECW1
Entrez Gene ID	701331
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Gene Type	protein-coding
Organism	Macaca mulatta(Rhesus monkey)

Homo sapiens (human)	HECW1	NP_055867.3
Pan troglodytes (chimpanzee)	HECW1	XP_003318432.1
Canis lupus familiaris (dog)	HECW1	XP_005630981.1
Danio rerio (zebrafish)	hecw1b	NP_001139236.1
Mus musculus (house mouse)	Hecw1	NP_001074817.3
Xenopus tropicalis (tropical clawed frog)	hecw1	NP_001123750.1
	HECW1	XP_001096775.1
Rattus norvegicus (Norway rat)	Hecw1	NP_001099587.1
Gallus gallus (chicken)	HECW1	XP_004939648.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

HECW1 cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene

Clones

gRNAs

Related articles in PubMed

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