hecw1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	hecw1
Entrez Gene ID	100170498
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
General protein information	Preferred Names HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase HECW1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018094564.2	XP_017950053.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_018094565.2	XP_017950054.1	E3 ubiquitin-protein ligase HECW1 isoform X2
NM_001130278.1	NP_001123750.1	E3 ubiquitin-protein ligase HECW1
XM_018094564.1	XP_017950053.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_018094565.1	XP_017950054.1	E3 ubiquitin-protein ligase HECW1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	HECW1	NP_055867.3
Pan troglodytes (chimpanzee)	HECW1	XP_003318432.1
Canis lupus familiaris (dog)	HECW1	XP_005630981.1
Danio rerio (zebrafish)	hecw1b	NP_001139236.1
Mus musculus (house mouse)	Hecw1	NP_001074817.3
Xenopus tropicalis (tropical clawed frog)	hecw1	NP_001123750.1
	HECW1	XP_001096775.1
Rattus norvegicus (Norway rat)	Hecw1	NP_001099587.1
Gallus gallus (chicken)	HECW1	XP_004939648.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hecw1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hecw1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa44866	XM_018094564.1 Latest version!	Xenopus tropicalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (hecw1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OXa44867	XM_018094565.1 Latest version!	Xenopus tropicalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (hecw1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OXa59546	XM_018094564.2 Latest version!	Xenopus tropicalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (hecw1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa59547	XM_018094565.2 Latest version!	Xenopus tropicalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (hecw1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa22181	NM_001130278.1 Latest version!	Xenopus tropicalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (hecw1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hecw1 ( XM_018094564.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950053.1 Xenopus tropicalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (hecw1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa44866
Clone ID Related Accession (Same CDS sequence)	XM_018094564.1
Accession Version	XM_018094564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTATT CTGCTCGGAC TCTCATTATA CAGAATTTGT ACCAAAACAG GTTCCTTGGT 
TTGGCTGCCA TGGCTTCTCC TTCAAGGAAT TCTCAGAATA GGCGTCGATG CAAAGAACCT 
ATGAGGTATA GCTACAATCC TGACCAGTTT CATAATATGG ATATTAGGAA CAACCCCCAT 
GAAGGAGTAA CCATGCCACG TTCCACAAGT GACACTGACC TGGTAACTTC TGATAGTCGG 
TCAACTCTGA TGGTTAGTAG TTCTTACTAC TCAATAGGAC ACTCACAGGA TCTTGTAATC 
TACTGGGACA TAAAAGAAGA AGTGGATGCT GGAGACTGGA TTGGCATGTA TCTAATTGAT 
GAAGTCTTAT CAGAAAACTT CTTGGATTAC AAGAATCGTG GTGTGAATGG ATCTCACAGA 
GGTCAGATAG TCTGGAAGAT TGAGGCCAGC TCTTACTTTG TTGAGTCTGA AACAAAGATT 
TGTTTCAAAT ACTACCATGG CGTAAGTGGA GCTCTACGTG CTACAACTCC CAGCATCACT 
GTGAAAAATC CAACTGCCCC TATCTTTAAA AACATCAACA ATGAAGAAAC AACACAGGGG 
CAAGGAAGTC GCAGACTCAT AAGTTTTACC TTGTCAGATT TCCAAGCTCT GGGTTTAAAG 
AAAGGAATGT TCTTCAATCC GGATCCTTAC CTGAAGATTT CAATCCAGCC TGGAAAGCAT 
AGTATCTTCC CAGCCCTGCC TCACCATGGA CAGGAGAAGA GATCAAAGAT TGTATGCAAC 
ACTGTTAATC CTGTCTGGAA AAGAGAGCTT TTCAGTTTTG TCTCCCTTCC AACTGACGTA 
TTGGAAATTG AAGTAAAGGA TAAATTTGCC AAAAGCCGTC CAATCATCAA ACGATTTTTG 
GGAAAGCTCT CTATGCCAGT ACAGCGCCTG CTGGAAAGGC ATGCAATTGG AGATCGTGTT 
GTGAGTTACA CCTTAGGTCG CAGACTTCCT ACAGATCACG TCAGTGGACA ACTGCAGTTC 
CGATTTGAAA TAACATCATC CATTCACCCT GATGATGAAG AGATCTCAGT GTCTTCAGAC 
ACAGTGCCAG GTACAGCTTC TGCCAGTCCA CTTCACAACA CAAATGAGGA GACCCCTATT 
GAATCACAGA GAAGAGCTTC GGTTGCAACT GAATGTTCCT CTCAAGAATT GCCAAATGAA 
TGTATTTCTG CAAATGGTCA TGACAAGAAC TCAGCAAGTG GCAGCAGGTC CACCAGTCAA 
AACAGCTTGC ACAAAAACAT CACAGGAAGT GCCATGGCAA ATGCGTCTGG AGCAGAACTT 
ACAGAAAACT TGGATTCTGG CCAAGCTGAG ATGACTTTAC CTGTTGACTT CATAGATTCT 
GTTCAGGAGC AAGCAGCGTG TGTTGATTCT GAAGCCAAAT TAGAGAACAT TGTAGTTTCT 
GTTGCTGCTG CTGCTGCTGC TGGGCAAAAT GAAAATCCAG AGATCCTGGA TGTAGATGAA 
GAAAATATGT TACTTGAAGC CAGTAAAGAT CAAGAGAAAG CACCAGAGGA ACCAGAGGGA 
GGGTGTCCCT TACCAGATGG TGAACCAAAA CTTAGAAATA CGGTAAAAAG GAAAAACAGG 
CCTTGTTCAT TGCCAGTGTC AGAGCTGGAG ACAGTCATCG CATCAGCATG TGGAGAGCCT 
GAAACCCCTC GCACACATTA CATTAGGATT CATAATTTAC TCCATAGTCT GCCTTCAGCA 
CAGATGTCAG GTGATGGAGA TGAGGAAGAG CAAAACAGTG TTGATCTTGA TGAAGAATCC 
ACAGTTAAGG AAACTTCAGA AAAAGATATT GTTAGTGAAA CTGAAACTGT TGCTCCTGAT 
CCTTCATCTG AAGTTGTCAT TGATGAAAAT GATGATGTTG AAAACAGCAG GAGCCCTTTA 
CCTCTTGATA GCACGAGTCC TCATCTCCCT TCCTGTAACC ATAATTGCCT GGATGTGGAG 
TTGCTTAGAA CAGAAAGTGA AAGTGACTCA AGTGCTAGGG TCCATGGAGA CCACGAGTGT 
GACACTTGTG ACACATCTTG TTACAGCACA TCCTGTTACA GCACATCCTG CTACAGCACC 
TCATGTTATA GCCCCTCATG CTATGATAGT CACAACCGCT TTTCCAGTCA CACCCGCTTC 
TCTTCAGTGG ACAGTGCTAG AATGTCTGAA AGTACTGTCT TTTCTTCCCA GGATGATGAG 
GAGGATGAGA ACAGTGCTTT TGAATCTATG CCAGATTCAA ATCAAAGCCC AGAGCCTGAA 
AGAGACCAGG CTGATGGTTC TAATCAATGG TCTGATGATA CCTCTGGTCA AAGTGTTAAT 
TCAGAAAGGA CTCTTGGTGT CCTGGAATCT CCTGTAGCTG GGCCAAGCAA TAGAACAGAA 
GGGGATTGTA CCTTACCTAA CACCACTCCG TCTGTAACTC AGCTACCATC TCTGAGGGCA 
GATCATCACC ACTATCCAAC TATTGATGAA CCTCTTCCAC CAAACTGGGA AGCTCGTATT 
GACAGTCATG GAAGAGTTTT TTATGTAGAC CATGTCAATC GAACCACAAC GTGGCAGCGT 
CCAACAACAG CAGCAACACC AGATGGTATA AGCAGATCTG GATCTATCCA ACAGATGGAG 
CAACTGAACC GCAGGTATCA GAATATCCAG AGGACTATTG CAACAGAAAG AACAGAAGAA 
GATTCTTTAG GTCGTAATGA CAGGGCACCC GGAGTTGGTG ATTCGGAGAC AGATCTGTCT 
CAGCCAAACA CAGAGTTACG AAGAGAGGGA TCCCTAACAC CCATAAATTC CCAGAAAATT 
ACGTTACTGC TACAATCCCC AGCTGTCAAG TTTATAACCA ACCCAGAGTT TTTTACTATA 
CTGCATGCAA ACTATAGTGC CTACCGAGTT TTTACAAACA GCACCTGCTT GAAGCACATG 
GTTCTGAAAA TCCGAAGGGA TGCAAGGAAC TTTGAACGCT ACCAGCATAA CAGGGACTTG 
GTGAACTTTA TTAACATGTT TGCCGACACC CGGCTAGAGT TGCCACGAGG ATGGGAGATC 
AAAACTGACC AACAAGGAAA GTCTTTCTTT GTAGACCACA ACAGTCGAGC AACAACATTT 
ATTGACCCAA GAATTCCACT TCAAAATGGG CGCCTTCCAA ATCACCTGAC ACACAGACAG 
CACCTTCAAA GGCTTAGAAG TTATAGCGCT GGAGAGGCTT CAGAAGTCTC CAGAAATAGG 
GGAGCTTCTT TATTAGCCAG ACCTGGCCAT AGTTTAGTCA CAGCAGTTCG AAACCAGCAT 
CAACATGAAG CTGTACCTCT AGCCTACAAT GATAAGATTG TTGCTTTTCT GCGTCAGCCG 
AATATTTTTG AGATGCTACA AGAACGTCAA CCAAGTTTAG CCAGAAATCA TGCACTCAGG 
GAAAAGATTC ATTACATACG AACAGAAGGG ACTCATGGAC TGGAGAAATT ATCATGTGAT 
GCAGATTTGG TAATACTGTT AAGCCTTTTT GAAGAAGATA TAATGTCATA TATCCCTCTA 
CAAGCTGCCT TCCATCCAGG ATACAGCTTT TCCCCTCGCT GTTCACCTGG CTCCTCACCT 
CAGAACTCTC CAGGGTTACA GCGTGCCAGT GCCAGGGCGC CCTCCCCATA CAGAAGAGAT 
TTTGAAGCCA AGTTAAGAAA TTTCTACAGA AAACTTGAAG CCAAAGGGTA TGGTCAAGGC 
CCAGGAAAAA TTAAACTCAT TATTCGCCGG GATCATCTTT TGGAAGGAAC CTTCAACCAA 
GTAATGGCGT ATTCCCGCAA AGAGCTCCAG AGGAATAAGC TGTATATCAC ATTTGTTGGA 
GAGGAAGGCC TGGATTACAG CGGCCCATCT AGAGAGTTCT TCTTTCTTCT CTCTCAGGAG 
CTCTTTAATC CCTACTATGG GCTGTTTGAA TATTCAGCAA ATGATACGTA TACTGTACAA 
ATCAGCCCTA TGTCAGCTTT TGTTGAGAAC CACTTGGAAT GGTTCCGGTT TAGTGGGAGA 
ATCCTTGGAC TTGCCCTTAT TCATCAGTAT CTGCTGGATG CCTTCTTTAC AAGACCCTTT 
TATAAGGCCC TTCTACGGCT TCCCTGTGAC CTCAGTGACC TTGAATACCT AGATGAAGAA 
TTTCATCAGA GTTTACAGTG GATGAAGGAC AATGATATAA CTGATATCCT GGATCTTACA 
TTCACTGTCA ATGAGGAGGT GTTTGGGCAG GTCACTGAAA GAGAGCTCAA GTCTGGGGGT 
GCAAATATTC AGGTTTCAGA AAAAAATAAG AAAGAATATA TTGAGAAAAT GGTGAAATGG 
CGAGTAGAGC GAGGAGTCGT ACAGCAGACA GAAGCCCTTG TACGGGGATT TTATGAGGTG 
GTAGATTCAA GGCTTGTATC TGTATTTGAT GCAAGAGAAC TTGAGCTGGT AATTGCAGGA 
ACTGCTGAAA TTGACCTCAA TGACTGGCGC AATAACACAG AGTACCGAGG AGGATATCAT 
GATGGCCATA TTGTCATCCG GTGGTTCTGG GCAGCAGTGG AAAGGTTCAA CAATGAACAA 
AGGTTACGAC TCTTGCAGTT TGTCACTGGG ACATCTAGTG TGCCATATGA AGGGTTTGCA 
GCTCTGCGAG GAAGCAACGG GCTGAGGCGA TTCTGCATAG AAAAATGGGG GAAAATAACA 
TCTCTTCCAA GGGCACACAC GTGCTTCAAT CGCTTGGATC TTCCTCCATA TCCTTCTTAT 
TCAATGTTAC ATGAAAAATT GTTAATAGCA GTCGAGGAGA CAAGCACCTT TGGACTTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950053.1
CDS	230..5032
Translation	MIYSARTLIIQNLYQNRFLGLAAMASPSRNSQNRRRCKEPMRYSYNPDQFHNMDIRNNPHEGVTMPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIVWKIEASSYFVESETKICFKYYHGVSGALRATTPSITVKNPTAPIFKNINNEETTQGQGSRRLISFTLSDFQALGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKIVCNTVNPVWKRELFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISVSSDTVPGTASASPLHNTNEETPIESQRRASVATECSSQELPNECISANGHDKNSASGSRSTSQNSLHKNITGSAMANASGAELTENLDSGQAEMTLPVDFIDSVQEQAACVDSEAKLENIVVSVAAAAAAGQNENPEILDVDEENMLLEASKDQEKAPEEPEGGCPLPDGEPKLRNTVKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSGDGDEEEQNSVDLDEESTVKETSEKDIVSETETVAPDPSSEVVIDENDDVENSRSPLPLDSTSPHLPSCNHNCLDVELLRTESESDSSARVHGDHECDTCDTSCYSTSCYSTSCYSTSCYSPSCYDSHNRFSSHTRFSSVDSARMSESTVFSSQDDEEDENSAFESMPDSNQSPEPERDQADGSNQWSDDTSGQSVNSERTLGVLESPVAGPSNRTEGDCTLPNTTPSVTQLPSLRADHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTTAATPDGISRSGSIQQMEQLNRRYQNIQRTIATERTEEDSLGRNDRAPGVGDSETDLSQPNTELRREGSLTPINSQKITLLLQSPAVKFITNPEFFTILHANYSAYRVFTNSTCLKHMVLKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVTAVRNQHQHEAVPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPGSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNDITDILDLTFTVNEEVFGQVTERELKSGGANIQVSEKNKKEYIEKMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLHEKLLIAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_018094564.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (hecw1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094564.1

ORF Insert Sequence:

ATGATTTATT CTGCTCGGAC TCTCATTATA CAGAATTTGT ACCAAAACAG GTTCCTTGGT 
TTGGCTGCCA TGGCTTCTCC TTCAAGGAAT TCTCAGAATA GGCGTCGATG CAAAGAACCT 
ATGAGGTATA GCTACAATCC TGACCAGTTT CATAATATGG ATATTAGGAA CAACCCCCAT 
GAAGGAGTAA CCATGCCACG TTCCACAAGT GACACTGACC TGGTAACTTC TGATAGTCGG 
TCAACTCTGA TGGTTAGTAG TTCTTACTAC TCAATAGGAC ACTCACAGGA TCTTGTAATC 
TACTGGGACA TAAAAGAAGA AGTGGATGCT GGAGACTGGA TTGGCATGTA TCTAATTGAT 
GAAGTCTTAT CAGAAAACTT CTTGGATTAC AAGAATCGTG GTGTGAATGG ATCTCACAGA 
GGTCAGATAG TCTGGAAGAT TGAGGCCAGC TCTTACTTTG TTGAGTCTGA AACAAAGATT 
TGTTTCAAAT ACTACCATGG CGTAAGTGGA GCTCTACGTG CTACAACTCC CAGCATCACT 
GTGAAAAATC CAACTGCCCC TATCTTTAAA AACATCAACA ATGAAGAAAC AACACAGGGG 
CAAGGAAGTC GCAGACTCAT AAGTTTTACC TTGTCAGATT TCCAAGCTCT GGGTTTAAAG 
AAAGGAATGT TCTTCAATCC GGATCCTTAC CTGAAGATTT CAATCCAGCC TGGAAAGCAT 
AGTATCTTCC CAGCCCTGCC TCACCATGGA CAGGAGAAGA GATCAAAGAT TGTATGCAAC 
ACTGTTAATC CTGTCTGGAA AAGAGAGCTT TTCAGTTTTG TCTCCCTTCC AACTGACGTA 
TTGGAAATTG AAGTAAAGGA TAAATTTGCC AAAAGCCGTC CAATCATCAA ACGATTTTTG 
GGAAAGCTCT CTATGCCAGT ACAGCGCCTG CTGGAAAGGC ATGCAATTGG AGATCGTGTT 
GTGAGTTACA CCTTAGGTCG CAGACTTCCT ACAGATCACG TCAGTGGACA ACTGCAGTTC 
CGATTTGAAA TAACATCATC CATTCACCCT GATGATGAAG AGATCTCAGT GTCTTCAGAC 
ACAGTGCCAG GTACAGCTTC TGCCAGTCCA CTTCACAACA CAAATGAGGA GACCCCTATT 
GAATCACAGA GAAGAGCTTC GGTTGCAACT GAATGTTCCT CTCAAGAATT GCCAAATGAA 
TGTATTTCTG CAAATGGTCA TGACAAGAAC TCAGCAAGTG GCAGCAGGTC CACCAGTCAA 
AACAGCTTGC ACAAAAACAT CACAGGAAGT GCCATGGCAA ATGCGTCTGG AGCAGAACTT 
ACAGAAAACT TGGATTCTGG CCAAGCTGAG ATGACTTTAC CTGTTGACTT CATAGATTCT 
GTTCAGGAGC AAGCAGCGTG TGTTGATTCT GAAGCCAAAT TAGAGAACAT TGTAGTTTCT 
GTTGCTGCTG CTGCTGCTGC TGGGCAAAAT GAAAATCCAG AGATCCTGGA TGTAGATGAA 
GAAAATATGT TACTTGAAGC CAGTAAAGAT CAAGAGAAAG CACCAGAGGA ACCAGAGGGA 
GGGTGTCCCT TACCAGATGG TGAACCAAAA CTTAGAAATA CGGTAAAAAG GAAAAACAGG 
CCTTGTTCAT TGCCAGTGTC AGAGCTGGAG ACAGTCATCG CATCAGCATG TGGAGAGCCT 
GAAACCCCTC GCACACATTA CATTAGGATT CATAATTTAC TCCATAGTCT GCCTTCAGCA 
CAGATGTCAG GTGATGGAGA TGAGGAAGAG CAAAACAGTG TTGATCTTGA TGAAGAATCC 
ACAGTTAAGG AAACTTCAGA AAAAGATATT GTTAGTGAAA CTGAAACTGT TGCTCCTGAT 
CCTTCATCTG AAGTTGTCAT TGATGAAAAT GATGATGTTG AAAACAGCAG GAGCCCTTTA 
CCTCTTGATA GCACGAGTCC TCATCTCCCT TCCTGTAACC ATAATTGCCT GGATGTGGAG 
TTGCTTAGAA CAGAAAGTGA AAGTGACTCA AGTGCTAGGG TCCATGGAGA CCACGAGTGT 
GACACTTGTG ACACATCTTG TTACAGCACA TCCTGTTACA GCACATCCTG CTACAGCACC 
TCATGTTATA GCCCCTCATG CTATGATAGT CACAACCGCT TTTCCAGTCA CACCCGCTTC 
TCTTCAGTGG ACAGTGCTAG AATGTCTGAA AGTACTGTCT TTTCTTCCCA GGATGATGAG 
GAGGATGAGA ACAGTGCTTT TGAATCTATG CCAGATTCAA ATCAAAGCCC AGAGCCTGAA 
AGAGACCAGG CTGATGGTTC TAATCAATGG TCTGATGATA CCTCTGGTCA AAGTGTTAAT 
TCAGAAAGGA CTCTTGGTGT CCTGGAATCT CCTGTAGCTG GGCCAAGCAA TAGAACAGAA 
GGGGATTGTA CCTTACCTAA CACCACTCCG TCTGTAACTC AGCTACCATC TCTGAGGGCA 
GATCATCACC ACTATCCAAC TATTGATGAA CCTCTTCCAC CAAACTGGGA AGCTCGTATT 
GACAGTCATG GAAGAGTTTT TTATGTAGAC CATGTCAATC GAACCACAAC GTGGCAGCGT 
CCAACAACAG CAGCAACACC AGATGGTATA AGCAGATCTG GATCTATCCA ACAGATGGAG 
CAACTGAACC GCAGGTATCA GAATATCCAG AGGACTATTG CAACAGAAAG AACAGAAGAA 
GATTCTTTAG GTCGTAATGA CAGGGCACCC GGAGTTGGTG ATTCGGAGAC AGATCTGTCT 
CAGCCAAACA CAGAGTTACG AAGAGAGGGA TCCCTAACAC CCATAAATTC CCAGAAAATT 
ACGTTACTGC TACAATCCCC AGCTGTCAAG TTTATAACCA ACCCAGAGTT TTTTACTATA 
CTGCATGCAA ACTATAGTGC CTACCGAGTT TTTACAAACA GCACCTGCTT GAAGCACATG 
GTTCTGAAAA TCCGAAGGGA TGCAAGGAAC TTTGAACGCT ACCAGCATAA CAGGGACTTG 
GTGAACTTTA TTAACATGTT TGCCGACACC CGGCTAGAGT TGCCACGAGG ATGGGAGATC 
AAAACTGACC AACAAGGAAA GTCTTTCTTT GTAGACCACA ACAGTCGAGC AACAACATTT 
ATTGACCCAA GAATTCCACT TCAAAATGGG CGCCTTCCAA ATCACCTGAC ACACAGACAG 
CACCTTCAAA GGCTTAGAAG TTATAGCGCT GGAGAGGCTT CAGAAGTCTC CAGAAATAGG 
GGAGCTTCTT TATTAGCCAG ACCTGGCCAT AGTTTAGTCA CAGCAGTTCG AAACCAGCAT 
CAACATGAAG CTGTACCTCT AGCCTACAAT GATAAGATTG TTGCTTTTCT GCGTCAGCCG 
AATATTTTTG AGATGCTACA AGAACGTCAA CCAAGTTTAG CCAGAAATCA TGCACTCAGG 
GAAAAGATTC ATTACATACG AACAGAAGGG ACTCATGGAC TGGAGAAATT ATCATGTGAT 
GCAGATTTGG TAATACTGTT AAGCCTTTTT GAAGAAGATA TAATGTCATA TATCCCTCTA 
CAAGCTGCCT TCCATCCAGG ATACAGCTTT TCCCCTCGCT GTTCACCTGG CTCCTCACCT 
CAGAACTCTC CAGGGTTACA GCGTGCCAGT GCCAGGGCGC CCTCCCCATA CAGAAGAGAT 
TTTGAAGCCA AGTTAAGAAA TTTCTACAGA AAACTTGAAG CCAAAGGGTA TGGTCAAGGC 
CCAGGAAAAA TTAAACTCAT TATTCGCCGG GATCATCTTT TGGAAGGAAC CTTCAACCAA 
GTAATGGCGT ATTCCCGCAA AGAGCTCCAG AGGAATAAGC TGTATATCAC ATTTGTTGGA 
GAGGAAGGCC TGGATTACAG CGGCCCATCT AGAGAGTTCT TCTTTCTTCT CTCTCAGGAG 
CTCTTTAATC CCTACTATGG GCTGTTTGAA TATTCAGCAA ATGATACGTA TACTGTACAA 
ATCAGCCCTA TGTCAGCTTT TGTTGAGAAC CACTTGGAAT GGTTCCGGTT TAGTGGGAGA 
ATCCTTGGAC TTGCCCTTAT TCATCAGTAT CTGCTGGATG CCTTCTTTAC AAGACCCTTT 
TATAAGGCCC TTCTACGGCT TCCCTGTGAC CTCAGTGACC TTGAATACCT AGATGAAGAA 
TTTCATCAGA GTTTACAGTG GATGAAGGAC AATGATATAA CTGATATCCT GGATCTTACA 
TTCACTGTCA ATGAGGAGGT GTTTGGGCAG GTCACTGAAA GAGAGCTCAA GTCTGGGGGT 
GCAAATATTC AGGTTTCAGA AAAAAATAAG AAAGAATATA TTGAGAAAAT GGTGAAATGG 
CGAGTAGAGC GAGGAGTCGT ACAGCAGACA GAAGCCCTTG TACGGGGATT TTATGAGGTG 
GTAGATTCAA GGCTTGTATC TGTATTTGAT GCAAGAGAAC TTGAGCTGGT AATTGCAGGA 
ACTGCTGAAA TTGACCTCAA TGACTGGCGC AATAACACAG AGTACCGAGG AGGATATCAT 
GATGGCCATA TTGTCATCCG GTGGTTCTGG GCAGCAGTGG AAAGGTTCAA CAATGAACAA 
AGGTTACGAC TCTTGCAGTT TGTCACTGGG ACATCTAGTG TGCCATATGA AGGGTTTGCA 
GCTCTGCGAG GAAGCAACGG GCTGAGGCGA TTCTGCATAG AAAAATGGGG GAAAATAACA 
TCTCTTCCAA GGGCACACAC GTGCTTCAAT CGCTTGGATC TTCCTCCATA TCCTTCTTAT 
TCAATGTTAC ATGAAAAATT GTTAATAGCA GTCGAGGAGA CAAGCACCTT TGGACTTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hecw1 ( XM_018094565.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950054.1 Xenopus tropicalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (hecw1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa44867
Clone ID Related Accession (Same CDS sequence)	XM_018094565.1
Accession Version	XM_018094565.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTTAAGAAT TTGTACCAAA ACAGGTTCCT TGGTTTGGCT 
GCCATGGCTT CTCCTTCAAG GAATTCTCAG AATAGGCGTC GATGCAAAGA ACCTATGAGG 
TATAGCTACA ATCCTGACCA GTTTCATAAT ATGGATATTA GGAACAACCC CCATGAAGGA 
GTAACCATGC CACGTTCCAC AAGTGACACT GACCTGGTAA CTTCTGATAG TCGGTCAACT 
CTGATGGTTA GTAGTTCTTA CTACTCAATA GGACACTCAC AGGATCTTGT AATCTACTGG 
GACATAAAAG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTATCTAAT TGATGAAGTC 
TTATCAGAAA ACTTCTTGGA TTACAAGAAT CGTGGTGTGA ATGGATCTCA CAGAGGTCAG 
ATAGTCTGGA AGATTGAGGC CAGCTCTTAC TTTGTTGAGT CTGAAACAAA GATTTGTTTC 
AAATACTACC ATGGCGTAAG TGGAGCTCTA CGTGCTACAA CTCCCAGCAT CACTGTGAAA 
AATCCAACTG CCCCTATCTT TAAAAACATC AACAATGAAG AAACAACACA GGGGCAAGGA 
AGTCGCAGAC TCATAAGTTT TACCTTGTCA GATTTCCAAG CTCTGGGTTT AAAGAAAGGA 
ATGTTCTTCA ATCCGGATCC TTACCTGAAG ATTTCAATCC AGCCTGGAAA GCATAGTATC 
TTCCCAGCCC TGCCTCACCA TGGACAGGAG AAGAGATCAA AGATTGTATG CAACACTGTT 
AATCCTGTCT GGAAAAGAGA GCTTTTCAGT TTTGTCTCCC TTCCAACTGA CGTATTGGAA 
ATTGAAGTAA AGGATAAATT TGCCAAAAGC CGTCCAATCA TCAAACGATT TTTGGGAAAG 
CTCTCTATGC CAGTACAGCG CCTGCTGGAA AGGCATGCAA TTGGAGATCG TGTTGTGAGT 
TACACCTTAG GTCGCAGACT TCCTACAGAT CACGTCAGTG GACAACTGCA GTTCCGATTT 
GAAATAACAT CATCCATTCA CCCTGATGAT GAAGAGATCT CAGTGTCTTC AGACACAGTG 
CCAGGTACAG CTTCTGCCAG TCCACTTCAC AACACAAATG AGGAGACCCC TATTGAATCA 
CAGAGAAGAG CTTCGGTTGC AACTGAATGT TCCTCTCAAG AATTGCCAAA TGAATGTATT 
TCTGCAAATG GTCATGACAA GAACTCAGCA AGTGGCAGCA GGTCCACCAG TCAAAACAGC 
TTGCACAAAA ACATCACAGG AAGTGCCATG GCAAATGCGT CTGGAGCAGA ACTTACAGAA 
AACTTGGATT CTGGCCAAGC TGAGATGACT TTACCTGTTG ACTTCATAGA TTCTGTTCAG 
GAGCAAGCAG CGTGTGTTGA TTCTGAAGCC AAATTAGAGA ACATTGTAGT TTCTGTTGCT 
GCTGCTGCTG CTGCTGGGCA AAATGAAAAT CCAGAGATCC TGGATGTAGA TGAAGAAAAT 
ATGTTACTTG AAGCCAGTAA AGATCAAGAG AAAGCACCAG AGGAACCAGA GGGAGGGTGT 
CCCTTACCAG ATGGTGAACC AAAACTTAGA AATACGGTAA AAAGGAAAAA CAGGCCTTGT 
TCATTGCCAG TGTCAGAGCT GGAGACAGTC ATCGCATCAG CATGTGGAGA GCCTGAAACC 
CCTCGCACAC ATTACATTAG GATTCATAAT TTACTCCATA GTCTGCCTTC AGCACAGATG 
TCAGGTGATG GAGATGAGGA AGAGCAAAAC AGTGTTGATC TTGATGAAGA ATCCACAGTT 
AAGGAAACTT CAGAAAAAGA TATTGTTAGT GAAACTGAAA CTGTTGCTCC TGATCCTTCA 
TCTGAAGTTG TCATTGATGA AAATGATGAT GTTGAAAACA GCAGGAGCCC TTTACCTCTT 
GATAGCACGA GTCCTCATCT CCCTTCCTGT AACCATAATT GCCTGGATGT GGAGTTGCTT 
AGAACAGAAA GTGAAAGTGA CTCAAGTGCT AGGGTCCATG GAGACCACGA GTGTGACACT 
TGTGACACAT CTTGTTACAG CACATCCTGT TACAGCACAT CCTGCTACAG CACCTCATGT 
TATAGCCCCT CATGCTATGA TAGTCACAAC CGCTTTTCCA GTCACACCCG CTTCTCTTCA 
GTGGACAGTG CTAGAATGTC TGAAAGTACT GTCTTTTCTT CCCAGGATGA TGAGGAGGAT 
GAGAACAGTG CTTTTGAATC TATGCCAGAT TCAAATCAAA GCCCAGAGCC TGAAAGAGAC 
CAGGCTGATG GTTCTAATCA ATGGTCTGAT GATACCTCTG GTCAAAGTGT TAATTCAGAA 
AGGACTCTTG GTGTCCTGGA ATCTCCTGTA GCTGGGCCAA GCAATAGAAC AGAAGGGGAT 
TGTACCTTAC CTAACACCAC TCCGTCTGTA ACTCAGCTAC CATCTCTGAG GGCAGATCAT 
CACCACTATC CAACTATTGA TGAACCTCTT CCACCAAACT GGGAAGCTCG TATTGACAGT 
CATGGAAGAG TTTTTTATGT AGACCATGTC AATCGAACCA CAACGTGGCA GCGTCCAACA 
ACAGCAGCAA CACCAGATGG TATAAGCAGA TCTGGATCTA TCCAACAGAT GGAGCAACTG 
AACCGCAGGT ATCAGAATAT CCAGAGGACT ATTGCAACAG AAAGAACAGA AGAAGATTCT 
TTAGGTCGTA ATGACAGGGC ACCCGGAGTT GGTGATTCGG AGACAGATCT GTCTCAGCCA 
AACACAGAGT TACGAAGAGA GGGATCCCTA ACACCCATAA ATTCCCAGAA AATTACGTTA 
CTGCTACAAT CCCCAGCTGT CAAGTTTATA ACCAACCCAG AGTTTTTTAC TATACTGCAT 
GCAAACTATA GTGCCTACCG AGTTTTTACA AACAGCACCT GCTTGAAGCA CATGGTTCTG 
AAAATCCGAA GGGATGCAAG GAACTTTGAA CGCTACCAGC ATAACAGGGA CTTGGTGAAC 
TTTATTAACA TGTTTGCCGA CACCCGGCTA GAGTTGCCAC GAGGATGGGA GATCAAAACT 
GACCAACAAG GAAAGTCTTT CTTTGTAGAC CACAACAGTC GAGCAACAAC ATTTATTGAC 
CCAAGAATTC CACTTCAAAA TGGGCGCCTT CCAAATCACC TGACACACAG ACAGCACCTT 
CAAAGGCTTA GAAGTTATAG CGCTGGAGAG GCTTCAGAAG TCTCCAGAAA TAGGGGAGCT 
TCTTTATTAG CCAGACCTGG CCATAGTTTA GTCACAGCAG TTCGAAACCA GCATCAACAT 
GAAGCTGTAC CTCTAGCCTA CAATGATAAG ATTGTTGCTT TTCTGCGTCA GCCGAATATT 
TTTGAGATGC TACAAGAACG TCAACCAAGT TTAGCCAGAA ATCATGCACT CAGGGAAAAG 
ATTCATTACA TACGAACAGA AGGGACTCAT GGACTGGAGA AATTATCATG TGATGCAGAT 
TTGGTAATAC TGTTAAGCCT TTTTGAAGAA GATATAATGT CATATATCCC TCTACAAGCT 
GCCTTCCATC CAGGATACAG CTTTTCCCCT CGCTGTTCAC CTGGCTCCTC ACCTCAGAAC 
TCTCCAGGGT TACAGCGTGC CAGTGCCAGG GCGCCCTCCC CATACAGAAG AGATTTTGAA 
GCCAAGTTAA GAAATTTCTA CAGAAAACTT GAAGCCAAAG GGTATGGTCA AGGCCCAGGA 
AAAATTAAAC TCATTATTCG CCGGGATCAT CTTTTGGAAG GAACCTTCAA CCAAGTAATG 
GCGTATTCCC GCAAAGAGCT CCAGAGGAAT AAGCTGTATA TCACATTTGT TGGAGAGGAA 
GGCCTGGATT ACAGCGGCCC ATCTAGAGAG TTCTTCTTTC TTCTCTCTCA GGAGCTCTTT 
AATCCCTACT ATGGGCTGTT TGAATATTCA GCAAATGATA CGTATACTGT ACAAATCAGC 
CCTATGTCAG CTTTTGTTGA GAACCACTTG GAATGGTTCC GGTTTAGTGG GAGAATCCTT 
GGACTTGCCC TTATTCATCA GTATCTGCTG GATGCCTTCT TTACAAGACC CTTTTATAAG 
GCCCTTCTAC GGCTTCCCTG TGACCTCAGT GACCTTGAAT ACCTAGATGA AGAATTTCAT 
CAGAGTTTAC AGTGGATGAA GGACAATGAT ATAACTGATA TCCTGGATCT TACATTCACT 
GTCAATGAGG AGGTGTTTGG GCAGGTCACT GAAAGAGAGC TCAAGTCTGG GGGTGCAAAT 
ATTCAGGTTT CAGAAAAAAA TAAGAAAGAA TATATTGAGA AAATGGTGAA ATGGCGAGTA 
GAGCGAGGAG TCGTACAGCA GACAGAAGCC CTTGTACGGG GATTTTATGA GGTGGTAGAT 
TCAAGGCTTG TATCTGTATT TGATGCAAGA GAACTTGAGC TGGTAATTGC AGGAACTGCT 
GAAATTGACC TCAATGACTG GCGCAATAAC ACAGAGTACC GAGGAGGATA TCATGATGGC 
CATATTGTCA TCCGGTGGTT CTGGGCAGCA GTGGAAAGGT TCAACAATGA ACAAAGGTTA 
CGACTCTTGC AGTTTGTCAC TGGGACATCT AGTGTGCCAT ATGAAGGGTT TGCAGCTCTG 
CGAGGAAGCA ACGGGCTGAG GCGATTCTGC ATAGAAAAAT GGGGGAAAAT AACATCTCTT 
CCAAGGGCAC ACACGTGCTT CAATCGCTTG GATCTTCCTC CATATCCTTC TTATTCAATG 
TTACATGAAA AATTGTTAAT AGCAGTCGAG GAGACAAGCA CCTTTGGACT TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950054.1
CDS	294..5090
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQNRRRCKEPMRYSYNPDQFHNMDIRNNPHEGVTMPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIVWKIEASSYFVESETKICFKYYHGVSGALRATTPSITVKNPTAPIFKNINNEETTQGQGSRRLISFTLSDFQALGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKIVCNTVNPVWKRELFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISVSSDTVPGTASASPLHNTNEETPIESQRRASVATECSSQELPNECISANGHDKNSASGSRSTSQNSLHKNITGSAMANASGAELTENLDSGQAEMTLPVDFIDSVQEQAACVDSEAKLENIVVSVAAAAAAGQNENPEILDVDEENMLLEASKDQEKAPEEPEGGCPLPDGEPKLRNTVKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSGDGDEEEQNSVDLDEESTVKETSEKDIVSETETVAPDPSSEVVIDENDDVENSRSPLPLDSTSPHLPSCNHNCLDVELLRTESESDSSARVHGDHECDTCDTSCYSTSCYSTSCYSTSCYSPSCYDSHNRFSSHTRFSSVDSARMSESTVFSSQDDEEDENSAFESMPDSNQSPEPERDQADGSNQWSDDTSGQSVNSERTLGVLESPVAGPSNRTEGDCTLPNTTPSVTQLPSLRADHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTTAATPDGISRSGSIQQMEQLNRRYQNIQRTIATERTEEDSLGRNDRAPGVGDSETDLSQPNTELRREGSLTPINSQKITLLLQSPAVKFITNPEFFTILHANYSAYRVFTNSTCLKHMVLKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVTAVRNQHQHEAVPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPGSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNDITDILDLTFTVNEEVFGQVTERELKSGGANIQVSEKNKKEYIEKMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLHEKLLIAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_018094565.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (hecw1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094565.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTTAAGAAT TTGTACCAAA ACAGGTTCCT TGGTTTGGCT 
GCCATGGCTT CTCCTTCAAG GAATTCTCAG AATAGGCGTC GATGCAAAGA ACCTATGAGG 
TATAGCTACA ATCCTGACCA GTTTCATAAT ATGGATATTA GGAACAACCC CCATGAAGGA 
GTAACCATGC CACGTTCCAC AAGTGACACT GACCTGGTAA CTTCTGATAG TCGGTCAACT 
CTGATGGTTA GTAGTTCTTA CTACTCAATA GGACACTCAC AGGATCTTGT AATCTACTGG 
GACATAAAAG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTATCTAAT TGATGAAGTC 
TTATCAGAAA ACTTCTTGGA TTACAAGAAT CGTGGTGTGA ATGGATCTCA CAGAGGTCAG 
ATAGTCTGGA AGATTGAGGC CAGCTCTTAC TTTGTTGAGT CTGAAACAAA GATTTGTTTC 
AAATACTACC ATGGCGTAAG TGGAGCTCTA CGTGCTACAA CTCCCAGCAT CACTGTGAAA 
AATCCAACTG CCCCTATCTT TAAAAACATC AACAATGAAG AAACAACACA GGGGCAAGGA 
AGTCGCAGAC TCATAAGTTT TACCTTGTCA GATTTCCAAG CTCTGGGTTT AAAGAAAGGA 
ATGTTCTTCA ATCCGGATCC TTACCTGAAG ATTTCAATCC AGCCTGGAAA GCATAGTATC 
TTCCCAGCCC TGCCTCACCA TGGACAGGAG AAGAGATCAA AGATTGTATG CAACACTGTT 
AATCCTGTCT GGAAAAGAGA GCTTTTCAGT TTTGTCTCCC TTCCAACTGA CGTATTGGAA 
ATTGAAGTAA AGGATAAATT TGCCAAAAGC CGTCCAATCA TCAAACGATT TTTGGGAAAG 
CTCTCTATGC CAGTACAGCG CCTGCTGGAA AGGCATGCAA TTGGAGATCG TGTTGTGAGT 
TACACCTTAG GTCGCAGACT TCCTACAGAT CACGTCAGTG GACAACTGCA GTTCCGATTT 
GAAATAACAT CATCCATTCA CCCTGATGAT GAAGAGATCT CAGTGTCTTC AGACACAGTG 
CCAGGTACAG CTTCTGCCAG TCCACTTCAC AACACAAATG AGGAGACCCC TATTGAATCA 
CAGAGAAGAG CTTCGGTTGC AACTGAATGT TCCTCTCAAG AATTGCCAAA TGAATGTATT 
TCTGCAAATG GTCATGACAA GAACTCAGCA AGTGGCAGCA GGTCCACCAG TCAAAACAGC 
TTGCACAAAA ACATCACAGG AAGTGCCATG GCAAATGCGT CTGGAGCAGA ACTTACAGAA 
AACTTGGATT CTGGCCAAGC TGAGATGACT TTACCTGTTG ACTTCATAGA TTCTGTTCAG 
GAGCAAGCAG CGTGTGTTGA TTCTGAAGCC AAATTAGAGA ACATTGTAGT TTCTGTTGCT 
GCTGCTGCTG CTGCTGGGCA AAATGAAAAT CCAGAGATCC TGGATGTAGA TGAAGAAAAT 
ATGTTACTTG AAGCCAGTAA AGATCAAGAG AAAGCACCAG AGGAACCAGA GGGAGGGTGT 
CCCTTACCAG ATGGTGAACC AAAACTTAGA AATACGGTAA AAAGGAAAAA CAGGCCTTGT 
TCATTGCCAG TGTCAGAGCT GGAGACAGTC ATCGCATCAG CATGTGGAGA GCCTGAAACC 
CCTCGCACAC ATTACATTAG GATTCATAAT TTACTCCATA GTCTGCCTTC AGCACAGATG 
TCAGGTGATG GAGATGAGGA AGAGCAAAAC AGTGTTGATC TTGATGAAGA ATCCACAGTT 
AAGGAAACTT CAGAAAAAGA TATTGTTAGT GAAACTGAAA CTGTTGCTCC TGATCCTTCA 
TCTGAAGTTG TCATTGATGA AAATGATGAT GTTGAAAACA GCAGGAGCCC TTTACCTCTT 
GATAGCACGA GTCCTCATCT CCCTTCCTGT AACCATAATT GCCTGGATGT GGAGTTGCTT 
AGAACAGAAA GTGAAAGTGA CTCAAGTGCT AGGGTCCATG GAGACCACGA GTGTGACACT 
TGTGACACAT CTTGTTACAG CACATCCTGT TACAGCACAT CCTGCTACAG CACCTCATGT 
TATAGCCCCT CATGCTATGA TAGTCACAAC CGCTTTTCCA GTCACACCCG CTTCTCTTCA 
GTGGACAGTG CTAGAATGTC TGAAAGTACT GTCTTTTCTT CCCAGGATGA TGAGGAGGAT 
GAGAACAGTG CTTTTGAATC TATGCCAGAT TCAAATCAAA GCCCAGAGCC TGAAAGAGAC 
CAGGCTGATG GTTCTAATCA ATGGTCTGAT GATACCTCTG GTCAAAGTGT TAATTCAGAA 
AGGACTCTTG GTGTCCTGGA ATCTCCTGTA GCTGGGCCAA GCAATAGAAC AGAAGGGGAT 
TGTACCTTAC CTAACACCAC TCCGTCTGTA ACTCAGCTAC CATCTCTGAG GGCAGATCAT 
CACCACTATC CAACTATTGA TGAACCTCTT CCACCAAACT GGGAAGCTCG TATTGACAGT 
CATGGAAGAG TTTTTTATGT AGACCATGTC AATCGAACCA CAACGTGGCA GCGTCCAACA 
ACAGCAGCAA CACCAGATGG TATAAGCAGA TCTGGATCTA TCCAACAGAT GGAGCAACTG 
AACCGCAGGT ATCAGAATAT CCAGAGGACT ATTGCAACAG AAAGAACAGA AGAAGATTCT 
TTAGGTCGTA ATGACAGGGC ACCCGGAGTT GGTGATTCGG AGACAGATCT GTCTCAGCCA 
AACACAGAGT TACGAAGAGA GGGATCCCTA ACACCCATAA ATTCCCAGAA AATTACGTTA 
CTGCTACAAT CCCCAGCTGT CAAGTTTATA ACCAACCCAG AGTTTTTTAC TATACTGCAT 
GCAAACTATA GTGCCTACCG AGTTTTTACA AACAGCACCT GCTTGAAGCA CATGGTTCTG 
AAAATCCGAA GGGATGCAAG GAACTTTGAA CGCTACCAGC ATAACAGGGA CTTGGTGAAC 
TTTATTAACA TGTTTGCCGA CACCCGGCTA GAGTTGCCAC GAGGATGGGA GATCAAAACT 
GACCAACAAG GAAAGTCTTT CTTTGTAGAC CACAACAGTC GAGCAACAAC ATTTATTGAC 
CCAAGAATTC CACTTCAAAA TGGGCGCCTT CCAAATCACC TGACACACAG ACAGCACCTT 
CAAAGGCTTA GAAGTTATAG CGCTGGAGAG GCTTCAGAAG TCTCCAGAAA TAGGGGAGCT 
TCTTTATTAG CCAGACCTGG CCATAGTTTA GTCACAGCAG TTCGAAACCA GCATCAACAT 
GAAGCTGTAC CTCTAGCCTA CAATGATAAG ATTGTTGCTT TTCTGCGTCA GCCGAATATT 
TTTGAGATGC TACAAGAACG TCAACCAAGT TTAGCCAGAA ATCATGCACT CAGGGAAAAG 
ATTCATTACA TACGAACAGA AGGGACTCAT GGACTGGAGA AATTATCATG TGATGCAGAT 
TTGGTAATAC TGTTAAGCCT TTTTGAAGAA GATATAATGT CATATATCCC TCTACAAGCT 
GCCTTCCATC CAGGATACAG CTTTTCCCCT CGCTGTTCAC CTGGCTCCTC ACCTCAGAAC 
TCTCCAGGGT TACAGCGTGC CAGTGCCAGG GCGCCCTCCC CATACAGAAG AGATTTTGAA 
GCCAAGTTAA GAAATTTCTA CAGAAAACTT GAAGCCAAAG GGTATGGTCA AGGCCCAGGA 
AAAATTAAAC TCATTATTCG CCGGGATCAT CTTTTGGAAG GAACCTTCAA CCAAGTAATG 
GCGTATTCCC GCAAAGAGCT CCAGAGGAAT AAGCTGTATA TCACATTTGT TGGAGAGGAA 
GGCCTGGATT ACAGCGGCCC ATCTAGAGAG TTCTTCTTTC TTCTCTCTCA GGAGCTCTTT 
AATCCCTACT ATGGGCTGTT TGAATATTCA GCAAATGATA CGTATACTGT ACAAATCAGC 
CCTATGTCAG CTTTTGTTGA GAACCACTTG GAATGGTTCC GGTTTAGTGG GAGAATCCTT 
GGACTTGCCC TTATTCATCA GTATCTGCTG GATGCCTTCT TTACAAGACC CTTTTATAAG 
GCCCTTCTAC GGCTTCCCTG TGACCTCAGT GACCTTGAAT ACCTAGATGA AGAATTTCAT 
CAGAGTTTAC AGTGGATGAA GGACAATGAT ATAACTGATA TCCTGGATCT TACATTCACT 
GTCAATGAGG AGGTGTTTGG GCAGGTCACT GAAAGAGAGC TCAAGTCTGG GGGTGCAAAT 
ATTCAGGTTT CAGAAAAAAA TAAGAAAGAA TATATTGAGA AAATGGTGAA ATGGCGAGTA 
GAGCGAGGAG TCGTACAGCA GACAGAAGCC CTTGTACGGG GATTTTATGA GGTGGTAGAT 
TCAAGGCTTG TATCTGTATT TGATGCAAGA GAACTTGAGC TGGTAATTGC AGGAACTGCT 
GAAATTGACC TCAATGACTG GCGCAATAAC ACAGAGTACC GAGGAGGATA TCATGATGGC 
CATATTGTCA TCCGGTGGTT CTGGGCAGCA GTGGAAAGGT TCAACAATGA ACAAAGGTTA 
CGACTCTTGC AGTTTGTCAC TGGGACATCT AGTGTGCCAT ATGAAGGGTT TGCAGCTCTG 
CGAGGAAGCA ACGGGCTGAG GCGATTCTGC ATAGAAAAAT GGGGGAAAAT AACATCTCTT 
CCAAGGGCAC ACACGTGCTT CAATCGCTTG GATCTTCCTC CATATCCTTC TTATTCAATG 
TTACATGAAA AATTGTTAAT AGCAGTCGAG GAGACAAGCA CCTTTGGACT TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hecw1 ( XM_018094564.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950053.1 Xenopus tropicalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (hecw1), transcript variant X1, mRNA.

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CloneID	OXa59546
Clone ID Related Accession (Same CDS sequence)	XM_018094564.2
Accession Version	XM_018094564.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094564.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTATT CTGCTCGGAC TCTCATTATA CAGAATTTGT ACCAAAACAG GTTCCTTGGT 
TTGGCTGCCA TGGCTTCTCC TTCAAGGAAT TCTCAGAATA GGCGTCGATG CAAAGAACCT 
ATGAGGTATA GCTACAATCC TGACCAGTTT CATAATATGG ATATTAGGAA CAACCCCCAT 
GAAGGAGTAA CCATGCCACG TTCCACAAGT GACACTGACC TGGTAACTTC TGATAGTCGG 
TCAACTCTGA TGGTTAGTAG TTCTTACTAC TCAATAGGAC ACTCACAGGA TCTTGTAATC 
TACTGGGACA TAAAAGAAGA AGTGGATGCT GGAGACTGGA TTGGCATGTA TCTAATTGAT 
GAAGTCTTAT CAGAAAACTT CTTGGATTAC AAGAATCGTG GTGTGAATGG ATCTCACAGA 
GGTCAGATAG TCTGGAAGAT TGAGGCCAGC TCTTACTTTG TTGAGTCTGA AACAAAGATT 
TGTTTCAAAT ACTACCATGG CGTAAGTGGA GCTCTACGTG CTACAACTCC CAGCATCACT 
GTGAAAAATC CAACTGCCCC TATCTTTAAA AACATCAACA ATGAAGAAAC AACACAGGGG 
CAAGGAAGTC GCAGACTCAT AAGTTTTACC TTGTCAGATT TCCAAGCTCT GGGTTTAAAG 
AAAGGAATGT TCTTCAATCC GGATCCTTAC CTGAAGATTT CAATCCAGCC TGGAAAGCAT 
AGTATCTTCC CAGCCCTGCC TCACCATGGA CAGGAGAAGA GATCAAAGAT TGTATGCAAC 
ACTGTTAATC CTGTCTGGAA AAGAGAGCTT TTCAGTTTTG TCTCCCTTCC AACTGACGTA 
TTGGAAATTG AAGTAAAGGA TAAATTTGCC AAAAGCCGTC CAATCATCAA ACGATTTTTG 
GGAAAGCTCT CTATGCCAGT ACAGCGCCTG CTGGAAAGGC ATGCAATTGG AGATCGTGTT 
GTGAGTTACA CCTTAGGTCG CAGACTTCCT ACAGATCACG TCAGTGGACA ACTGCAGTTC 
CGATTTGAAA TAACATCATC CATTCACCCT GATGATGAAG AGATCTCAGT GTCTTCAGAC 
ACAGTGCCAG GTACAGCTTC TGCCAGTCCA CTTCACAACA CAAATGAGGA GACCCCTATT 
GAATCACAGA GAAGAGCTTC GGTTGCAACT GAATGTTCCT CTCAAGAATT GCCAAATGAA 
TGTATTTCTG CAAATGGTCA TGACAAGAAC TCAGCAAGTG GCAGCAGGTC CACCAGTCAA 
AACAGCTTGC ACAAAAACAT CACAGGAAGT GCCATGGCAA ATGCGTCTGG AGCAGAACTT 
ACAGAAAACT TGGATTCTGG CCAAGCTGAG ATGACTTTAC CTGTTGACTT CATAGATTCT 
GTTCAGGAGC AAGCAGCGTG TGTTGATTCT GAAGCCAAAT TAGAGAACAT TGTAGTTTCT 
GTTGCTGCTG CTGCTGCTGC TGGGCAAAAT GAAAATCCAG AGATCCTGGA TGTAGATGAA 
GAAAATATGT TACTTGAAGC CAGTAAAGAT CAAGAGAAAG CACCAGAGGA ACCAGAGGGA 
GGGTGTCCCT TACCAGATGG TGAACCAAAA CTTAGAAATA CGGTAAAAAG GAAAAACAGG 
CCTTGTTCAT TGCCAGTGTC AGAGCTGGAG ACAGTCATCG CATCAGCATG TGGAGAGCCT 
GAAACCCCTC GCACACATTA CATTAGGATT CATAATTTAC TCCATAGTCT GCCTTCAGCA 
CAGATGTCAG GTGATGGAGA TGAGGAAGAG CAAAACAGTG TTGATCTTGA TGAAGAATCC 
ACAGTTAAGG AAACTTCAGA AAAAGATATT GTTAGTGAAA CTGAAACTGT TGCTCCTGAT 
CCTTCATCTG AAGTTGTCAT TGATGAAAAT GATGATGTTG AAAACAGCAG GAGCCCTTTA 
CCTCTTGATA GCACGAGTCC TCATCTCCCT TCCTGTAACC ATAATTGCCT GGATGTGGAG 
TTGCTTAGAA CAGAAAGTGA AAGTGACTCA AGTGCTAGGG TCCATGGAGA CCACGAGTGT 
GACACTTGTG ACACATCTTG TTACAGCACA TCCTGTTACA GCACATCCTG CTACAGCACC 
TCATGTTATA GCCCCTCATG CTATGATAGT CACAACCGCT TTTCCAGTCA CACCCGCTTC 
TCTTCAGTGG ACAGTGCTAG AATGTCTGAA AGTACTGTCT TTTCTTCCCA GGATGATGAG 
GAGGATGAGA ACAGTGCTTT TGAATCTATG CCAGATTCAA ATCAAAGCCC AGAGCCTGAA 
AGAGACCAGG CTGATGGTTC TAATCAATGG TCTGATGATA CCTCTGGTCA AAGTGTTAAT 
TCAGAAAGGA CTCTTGGTGT CCTGGAATCT CCTGTAGCTG GGCCAAGCAA TAGAACAGAA 
GGGGATTGTA CCTTACCTAA CACCACTCCG TCTGTAACTC AGCTACCATC TCTGAGGGCA 
GATCATCACC ACTATCCAAC TATTGATGAA CCTCTTCCAC CAAACTGGGA AGCTCGTATT 
GACAGTCATG GAAGAGTTTT TTATGTAGAC CATGTCAATC GAACCACAAC GTGGCAGCGT 
CCAACAACAG CAGCAACACC AGATGGTATA AGCAGATCTG GATCTATCCA ACAGATGGAG 
CAACTGAACC GCAGGTATCA GAATATCCAG AGGACTATTG CAACAGAAAG AACAGAAGAA 
GATTCTTTAG GTCGTAATGA CAGGGCACCC GGAGTTGGTG ATTCGGAGAC AGATCTGTCT 
CAGCCAAACA CAGAGTTACG AAGAGAGGGA TCCCTAACAC CCATAAATTC CCAGAAAATT 
ACGTTACTGC TACAATCCCC AGCTGTCAAG TTTATAACCA ACCCAGAGTT TTTTACTATA 
CTGCATGCAA ACTATAGTGC CTACCGAGTT TTTACAAACA GCACCTGCTT GAAGCACATG 
GTTCTGAAAA TCCGAAGGGA TGCAAGGAAC TTTGAACGCT ACCAGCATAA CAGGGACTTG 
GTGAACTTTA TTAACATGTT TGCCGACACC CGGCTAGAGT TGCCACGAGG ATGGGAGATC 
AAAACTGACC AACAAGGAAA GTCTTTCTTT GTAGACCACA ACAGTCGAGC AACAACATTT 
ATTGACCCAA GAATTCCACT TCAAAATGGG CGCCTTCCAA ATCACCTGAC ACACAGACAG 
CACCTTCAAA GGCTTAGAAG TTATAGCGCT GGAGAGGCTT CAGAAGTCTC CAGAAATAGG 
GGAGCTTCTT TATTAGCCAG ACCTGGCCAT AGTTTAGTCA CAGCAGTTCG AAACCAGCAT 
CAACATGAAG CTGTACCTCT AGCCTACAAT GATAAGATTG TTGCTTTTCT GCGTCAGCCG 
AATATTTTTG AGATGCTACA AGAACGTCAA CCAAGTTTAG CCAGAAATCA TGCACTCAGG 
GAAAAGATTC ATTACATACG AACAGAAGGG ACTCATGGAT TGGAGAAATT ATCATGTGAT 
GCAGATTTGG TAATACTGTT AAGCCTTTTT GAAGAAGATA TAATGTCATA TATCCCTCTA 
CAAGCTGCCT TCCATCCAGG ATACAGCTTT TCCCCTCGCT GTTCACCTGG CTCCTCACCT 
CAGAACTCTC CAGGGTTACA GCGTGCCAGT GCCAGGGCGC CCTCCCCATA CAGAAGAGAT 
TTTGAAGCCA AGTTAAGAAA TTTCTACAGA AAACTTGAAG CCAAAGGGTA TGGTCAAGGC 
CCAGGAAAAA TTAAACTCAT TATTCGCCGG GATCATCTTT TGGAAGGAAC CTTCAACCAA 
GTAATGGCGT ATTCCCGCAA AGAGCTCCAG AGGAATAAGC TGTATATCAC ATTTGTTGGA 
GAGGAAGGCC TGGATTACAG CGGCCCATCT AGAGAGTTCT TCTTTCTTCT CTCTCAGGAG 
CTCTTTAATC CCTACTATGG GCTGTTTGAA TATTCAGCAA ATGATACGTA TACTGTACAA 
ATCAGCCCTA TGTCAGCTTT TGTTGAGAAC CACTTGGAAT GGTTCCGGTT TAGTGGGAGA 
ATCCTTGGAC TTGCCCTTAT TCATCAGTAT CTGCTGGATG CCTTCTTTAC AAGACCCTTT 
TATAAGGCCC TTCTACGGCT TCCCTGTGAC CTCAGTGACC TTGAATACCT AGATGAAGAA 
TTTCATCAGA GTTTACAGTG GATGAAGGAC AATGATATAA CTGATATCCT GGATCTTACA 
TTCACTGTCA ATGAGGAGGT GTTTGGGCAG GTCACTGAAA GAGAGCTCAA GTCTGGGGGT 
GCAAATATTC AGGTTTCAGA AAAAAATAAG AAAGAATATA TTGAGAAAAT GGTGAAATGG 
CGAGTAGAGC GAGGAGTCGT ACAGCAGACA GAAGCCCTTG TACGGGGATT TTATGAGGTG 
GTAGATTCAA GGCTTGTATC TGTATTTGAT GCAAGAGAAC TTGAGCTGGT AATTGCAGGA 
ACTGCTGAAA TTGACCTCAA TGACTGGCGC AATAACACAG AGTACCGAGG AGGATATCAT 
GATGGCCATA TTGTCATCCG GTGGTTCTGG GCAGCAGTGG AAAGGTTCAA CAATGAACAA 
AGGTTACGAC TCTTGCAGTT TGTCACTGGG ACATCTAGTG TGCCATATGA AGGGTTTGCA 
GCTCTGCGAG GAAGCAACGG GCTGAGGCGA TTCTGCATAG AAAAATGGGG GAAAATAACA 
TCTCTTCCAA GGGCACACAC GTGCTTCAAT CGCTTGGATC TTCCTCCATA TCCTTCTTAT 
TCAATGTTAC ATGAAAAATT GTTAATAGCA GTCGAGGAGA CAAGCACCTT TGGACTTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950053.1
CDS	226..5028
Translation	MIYSARTLIIQNLYQNRFLGLAAMASPSRNSQNRRRCKEPMRYSYNPDQFHNMDIRNNPHEGVTMPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIVWKIEASSYFVESETKICFKYYHGVSGALRATTPSITVKNPTAPIFKNINNEETTQGQGSRRLISFTLSDFQALGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKIVCNTVNPVWKRELFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISVSSDTVPGTASASPLHNTNEETPIESQRRASVATECSSQELPNECISANGHDKNSASGSRSTSQNSLHKNITGSAMANASGAELTENLDSGQAEMTLPVDFIDSVQEQAACVDSEAKLENIVVSVAAAAAAGQNENPEILDVDEENMLLEASKDQEKAPEEPEGGCPLPDGEPKLRNTVKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSGDGDEEEQNSVDLDEESTVKETSEKDIVSETETVAPDPSSEVVIDENDDVENSRSPLPLDSTSPHLPSCNHNCLDVELLRTESESDSSARVHGDHECDTCDTSCYSTSCYSTSCYSTSCYSPSCYDSHNRFSSHTRFSSVDSARMSESTVFSSQDDEEDENSAFESMPDSNQSPEPERDQADGSNQWSDDTSGQSVNSERTLGVLESPVAGPSNRTEGDCTLPNTTPSVTQLPSLRADHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTTAATPDGISRSGSIQQMEQLNRRYQNIQRTIATERTEEDSLGRNDRAPGVGDSETDLSQPNTELRREGSLTPINSQKITLLLQSPAVKFITNPEFFTILHANYSAYRVFTNSTCLKHMVLKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVTAVRNQHQHEAVPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPGSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNDITDILDLTFTVNEEVFGQVTERELKSGGANIQVSEKNKKEYIEKMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLHEKLLIAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_018094564.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (hecw1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094564.2

ORF Insert Sequence:

ATGATTTATT CTGCTCGGAC TCTCATTATA CAGAATTTGT ACCAAAACAG GTTCCTTGGT 
TTGGCTGCCA TGGCTTCTCC TTCAAGGAAT TCTCAGAATA GGCGTCGATG CAAAGAACCT 
ATGAGGTATA GCTACAATCC TGACCAGTTT CATAATATGG ATATTAGGAA CAACCCCCAT 
GAAGGAGTAA CCATGCCACG TTCCACAAGT GACACTGACC TGGTAACTTC TGATAGTCGG 
TCAACTCTGA TGGTTAGTAG TTCTTACTAC TCAATAGGAC ACTCACAGGA TCTTGTAATC 
TACTGGGACA TAAAAGAAGA AGTGGATGCT GGAGACTGGA TTGGCATGTA TCTAATTGAT 
GAAGTCTTAT CAGAAAACTT CTTGGATTAC AAGAATCGTG GTGTGAATGG ATCTCACAGA 
GGTCAGATAG TCTGGAAGAT TGAGGCCAGC TCTTACTTTG TTGAGTCTGA AACAAAGATT 
TGTTTCAAAT ACTACCATGG CGTAAGTGGA GCTCTACGTG CTACAACTCC CAGCATCACT 
GTGAAAAATC CAACTGCCCC TATCTTTAAA AACATCAACA ATGAAGAAAC AACACAGGGG 
CAAGGAAGTC GCAGACTCAT AAGTTTTACC TTGTCAGATT TCCAAGCTCT GGGTTTAAAG 
AAAGGAATGT TCTTCAATCC GGATCCTTAC CTGAAGATTT CAATCCAGCC TGGAAAGCAT 
AGTATCTTCC CAGCCCTGCC TCACCATGGA CAGGAGAAGA GATCAAAGAT TGTATGCAAC 
ACTGTTAATC CTGTCTGGAA AAGAGAGCTT TTCAGTTTTG TCTCCCTTCC AACTGACGTA 
TTGGAAATTG AAGTAAAGGA TAAATTTGCC AAAAGCCGTC CAATCATCAA ACGATTTTTG 
GGAAAGCTCT CTATGCCAGT ACAGCGCCTG CTGGAAAGGC ATGCAATTGG AGATCGTGTT 
GTGAGTTACA CCTTAGGTCG CAGACTTCCT ACAGATCACG TCAGTGGACA ACTGCAGTTC 
CGATTTGAAA TAACATCATC CATTCACCCT GATGATGAAG AGATCTCAGT GTCTTCAGAC 
ACAGTGCCAG GTACAGCTTC TGCCAGTCCA CTTCACAACA CAAATGAGGA GACCCCTATT 
GAATCACAGA GAAGAGCTTC GGTTGCAACT GAATGTTCCT CTCAAGAATT GCCAAATGAA 
TGTATTTCTG CAAATGGTCA TGACAAGAAC TCAGCAAGTG GCAGCAGGTC CACCAGTCAA 
AACAGCTTGC ACAAAAACAT CACAGGAAGT GCCATGGCAA ATGCGTCTGG AGCAGAACTT 
ACAGAAAACT TGGATTCTGG CCAAGCTGAG ATGACTTTAC CTGTTGACTT CATAGATTCT 
GTTCAGGAGC AAGCAGCGTG TGTTGATTCT GAAGCCAAAT TAGAGAACAT TGTAGTTTCT 
GTTGCTGCTG CTGCTGCTGC TGGGCAAAAT GAAAATCCAG AGATCCTGGA TGTAGATGAA 
GAAAATATGT TACTTGAAGC CAGTAAAGAT CAAGAGAAAG CACCAGAGGA ACCAGAGGGA 
GGGTGTCCCT TACCAGATGG TGAACCAAAA CTTAGAAATA CGGTAAAAAG GAAAAACAGG 
CCTTGTTCAT TGCCAGTGTC AGAGCTGGAG ACAGTCATCG CATCAGCATG TGGAGAGCCT 
GAAACCCCTC GCACACATTA CATTAGGATT CATAATTTAC TCCATAGTCT GCCTTCAGCA 
CAGATGTCAG GTGATGGAGA TGAGGAAGAG CAAAACAGTG TTGATCTTGA TGAAGAATCC 
ACAGTTAAGG AAACTTCAGA AAAAGATATT GTTAGTGAAA CTGAAACTGT TGCTCCTGAT 
CCTTCATCTG AAGTTGTCAT TGATGAAAAT GATGATGTTG AAAACAGCAG GAGCCCTTTA 
CCTCTTGATA GCACGAGTCC TCATCTCCCT TCCTGTAACC ATAATTGCCT GGATGTGGAG 
TTGCTTAGAA CAGAAAGTGA AAGTGACTCA AGTGCTAGGG TCCATGGAGA CCACGAGTGT 
GACACTTGTG ACACATCTTG TTACAGCACA TCCTGTTACA GCACATCCTG CTACAGCACC 
TCATGTTATA GCCCCTCATG CTATGATAGT CACAACCGCT TTTCCAGTCA CACCCGCTTC 
TCTTCAGTGG ACAGTGCTAG AATGTCTGAA AGTACTGTCT TTTCTTCCCA GGATGATGAG 
GAGGATGAGA ACAGTGCTTT TGAATCTATG CCAGATTCAA ATCAAAGCCC AGAGCCTGAA 
AGAGACCAGG CTGATGGTTC TAATCAATGG TCTGATGATA CCTCTGGTCA AAGTGTTAAT 
TCAGAAAGGA CTCTTGGTGT CCTGGAATCT CCTGTAGCTG GGCCAAGCAA TAGAACAGAA 
GGGGATTGTA CCTTACCTAA CACCACTCCG TCTGTAACTC AGCTACCATC TCTGAGGGCA 
GATCATCACC ACTATCCAAC TATTGATGAA CCTCTTCCAC CAAACTGGGA AGCTCGTATT 
GACAGTCATG GAAGAGTTTT TTATGTAGAC CATGTCAATC GAACCACAAC GTGGCAGCGT 
CCAACAACAG CAGCAACACC AGATGGTATA AGCAGATCTG GATCTATCCA ACAGATGGAG 
CAACTGAACC GCAGGTATCA GAATATCCAG AGGACTATTG CAACAGAAAG AACAGAAGAA 
GATTCTTTAG GTCGTAATGA CAGGGCACCC GGAGTTGGTG ATTCGGAGAC AGATCTGTCT 
CAGCCAAACA CAGAGTTACG AAGAGAGGGA TCCCTAACAC CCATAAATTC CCAGAAAATT 
ACGTTACTGC TACAATCCCC AGCTGTCAAG TTTATAACCA ACCCAGAGTT TTTTACTATA 
CTGCATGCAA ACTATAGTGC CTACCGAGTT TTTACAAACA GCACCTGCTT GAAGCACATG 
GTTCTGAAAA TCCGAAGGGA TGCAAGGAAC TTTGAACGCT ACCAGCATAA CAGGGACTTG 
GTGAACTTTA TTAACATGTT TGCCGACACC CGGCTAGAGT TGCCACGAGG ATGGGAGATC 
AAAACTGACC AACAAGGAAA GTCTTTCTTT GTAGACCACA ACAGTCGAGC AACAACATTT 
ATTGACCCAA GAATTCCACT TCAAAATGGG CGCCTTCCAA ATCACCTGAC ACACAGACAG 
CACCTTCAAA GGCTTAGAAG TTATAGCGCT GGAGAGGCTT CAGAAGTCTC CAGAAATAGG 
GGAGCTTCTT TATTAGCCAG ACCTGGCCAT AGTTTAGTCA CAGCAGTTCG AAACCAGCAT 
CAACATGAAG CTGTACCTCT AGCCTACAAT GATAAGATTG TTGCTTTTCT GCGTCAGCCG 
AATATTTTTG AGATGCTACA AGAACGTCAA CCAAGTTTAG CCAGAAATCA TGCACTCAGG 
GAAAAGATTC ATTACATACG AACAGAAGGG ACTCATGGAT TGGAGAAATT ATCATGTGAT 
GCAGATTTGG TAATACTGTT AAGCCTTTTT GAAGAAGATA TAATGTCATA TATCCCTCTA 
CAAGCTGCCT TCCATCCAGG ATACAGCTTT TCCCCTCGCT GTTCACCTGG CTCCTCACCT 
CAGAACTCTC CAGGGTTACA GCGTGCCAGT GCCAGGGCGC CCTCCCCATA CAGAAGAGAT 
TTTGAAGCCA AGTTAAGAAA TTTCTACAGA AAACTTGAAG CCAAAGGGTA TGGTCAAGGC 
CCAGGAAAAA TTAAACTCAT TATTCGCCGG GATCATCTTT TGGAAGGAAC CTTCAACCAA 
GTAATGGCGT ATTCCCGCAA AGAGCTCCAG AGGAATAAGC TGTATATCAC ATTTGTTGGA 
GAGGAAGGCC TGGATTACAG CGGCCCATCT AGAGAGTTCT TCTTTCTTCT CTCTCAGGAG 
CTCTTTAATC CCTACTATGG GCTGTTTGAA TATTCAGCAA ATGATACGTA TACTGTACAA 
ATCAGCCCTA TGTCAGCTTT TGTTGAGAAC CACTTGGAAT GGTTCCGGTT TAGTGGGAGA 
ATCCTTGGAC TTGCCCTTAT TCATCAGTAT CTGCTGGATG CCTTCTTTAC AAGACCCTTT 
TATAAGGCCC TTCTACGGCT TCCCTGTGAC CTCAGTGACC TTGAATACCT AGATGAAGAA 
TTTCATCAGA GTTTACAGTG GATGAAGGAC AATGATATAA CTGATATCCT GGATCTTACA 
TTCACTGTCA ATGAGGAGGT GTTTGGGCAG GTCACTGAAA GAGAGCTCAA GTCTGGGGGT 
GCAAATATTC AGGTTTCAGA AAAAAATAAG AAAGAATATA TTGAGAAAAT GGTGAAATGG 
CGAGTAGAGC GAGGAGTCGT ACAGCAGACA GAAGCCCTTG TACGGGGATT TTATGAGGTG 
GTAGATTCAA GGCTTGTATC TGTATTTGAT GCAAGAGAAC TTGAGCTGGT AATTGCAGGA 
ACTGCTGAAA TTGACCTCAA TGACTGGCGC AATAACACAG AGTACCGAGG AGGATATCAT 
GATGGCCATA TTGTCATCCG GTGGTTCTGG GCAGCAGTGG AAAGGTTCAA CAATGAACAA 
AGGTTACGAC TCTTGCAGTT TGTCACTGGG ACATCTAGTG TGCCATATGA AGGGTTTGCA 
GCTCTGCGAG GAAGCAACGG GCTGAGGCGA TTCTGCATAG AAAAATGGGG GAAAATAACA 
TCTCTTCCAA GGGCACACAC GTGCTTCAAT CGCTTGGATC TTCCTCCATA TCCTTCTTAT 
TCAATGTTAC ATGAAAAATT GTTAATAGCA GTCGAGGAGA CAAGCACCTT TGGACTTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hecw1 ( XM_018094565.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950054.1 Xenopus tropicalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (hecw1), transcript variant X2, mRNA.

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CloneID	OXa59547
Clone ID Related Accession (Same CDS sequence)	XM_018094565.2
Accession Version	XM_018094565.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTTAAGAAT TTGTACCAAA ACAGGTTCCT TGGTTTGGCT 
GCCATGGCTT CTCCTTCAAG GAATTCTCAG AATAGGCGTC GATGCAAAGA ACCTATGAGG 
TATAGCTACA ATCCTGACCA GTTTCATAAT ATGGATATTA GGAACAACCC CCATGAAGGA 
GTAACCATGC CACGTTCCAC AAGTGACACT GACCTGGTAA CTTCTGATAG TCGGTCAACT 
CTGATGGTTA GTAGTTCTTA CTACTCAATA GGACACTCAC AGGATCTTGT AATCTACTGG 
GACATAAAAG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTATCTAAT TGATGAAGTC 
TTATCAGAAA ACTTCTTGGA TTACAAGAAT CGTGGTGTGA ATGGATCTCA CAGAGGTCAG 
ATAGTCTGGA AGATTGAGGC CAGCTCTTAC TTTGTTGAGT CTGAAACAAA GATTTGTTTC 
AAATACTACC ATGGCGTAAG TGGAGCTCTA CGTGCTACAA CTCCCAGCAT CACTGTGAAA 
AATCCAACTG CCCCTATCTT TAAAAACATC AACAATGAAG AAACAACACA GGGGCAAGGA 
AGTCGCAGAC TCATAAGTTT TACCTTGTCA GATTTCCAAG CTCTGGGTTT AAAGAAAGGA 
ATGTTCTTCA ATCCGGATCC TTACCTGAAG ATTTCAATCC AGCCTGGAAA GCATAGTATC 
TTCCCAGCCC TGCCTCACCA TGGACAGGAG AAGAGATCAA AGATTGTATG CAACACTGTT 
AATCCTGTCT GGAAAAGAGA GCTTTTCAGT TTTGTCTCCC TTCCAACTGA CGTATTGGAA 
ATTGAAGTAA AGGATAAATT TGCCAAAAGC CGTCCAATCA TCAAACGATT TTTGGGAAAG 
CTCTCTATGC CAGTACAGCG CCTGCTGGAA AGGCATGCAA TTGGAGATCG TGTTGTGAGT 
TACACCTTAG GTCGCAGACT TCCTACAGAT CACGTCAGTG GACAACTGCA GTTCCGATTT 
GAAATAACAT CATCCATTCA CCCTGATGAT GAAGAGATCT CAGTGTCTTC AGACACAGTG 
CCAGGTACAG CTTCTGCCAG TCCACTTCAC AACACAAATG AGGAGACCCC TATTGAATCA 
CAGAGAAGAG CTTCGGTTGC AACTGAATGT TCCTCTCAAG AATTGCCAAA TGAATGTATT 
TCTGCAAATG GTCATGACAA GAACTCAGCA AGTGGCAGCA GGTCCACCAG TCAAAACAGC 
TTGCACAAAA ACATCACAGG AAGTGCCATG GCAAATGCGT CTGGAGCAGA ACTTACAGAA 
AACTTGGATT CTGGCCAAGC TGAGATGACT TTACCTGTTG ACTTCATAGA TTCTGTTCAG 
GAGCAAGCAG CGTGTGTTGA TTCTGAAGCC AAATTAGAGA ACATTGTAGT TTCTGTTGCT 
GCTGCTGCTG CTGCTGGGCA AAATGAAAAT CCAGAGATCC TGGATGTAGA TGAAGAAAAT 
ATGTTACTTG AAGCCAGTAA AGATCAAGAG AAAGCACCAG AGGAACCAGA GGGAGGGTGT 
CCCTTACCAG ATGGTGAACC AAAACTTAGA AATACGGTAA AAAGGAAAAA CAGGCCTTGT 
TCATTGCCAG TGTCAGAGCT GGAGACAGTC ATCGCATCAG CATGTGGAGA GCCTGAAACC 
CCTCGCACAC ATTACATTAG GATTCATAAT TTACTCCATA GTCTGCCTTC AGCACAGATG 
TCAGGTGATG GAGATGAGGA AGAGCAAAAC AGTGTTGATC TTGATGAAGA ATCCACAGTT 
AAGGAAACTT CAGAAAAAGA TATTGTTAGT GAAACTGAAA CTGTTGCTCC TGATCCTTCA 
TCTGAAGTTG TCATTGATGA AAATGATGAT GTTGAAAACA GCAGGAGCCC TTTACCTCTT 
GATAGCACGA GTCCTCATCT CCCTTCCTGT AACCATAATT GCCTGGATGT GGAGTTGCTT 
AGAACAGAAA GTGAAAGTGA CTCAAGTGCT AGGGTCCATG GAGACCACGA GTGTGACACT 
TGTGACACAT CTTGTTACAG CACATCCTGT TACAGCACAT CCTGCTACAG CACCTCATGT 
TATAGCCCCT CATGCTATGA TAGTCACAAC CGCTTTTCCA GTCACACCCG CTTCTCTTCA 
GTGGACAGTG CTAGAATGTC TGAAAGTACT GTCTTTTCTT CCCAGGATGA TGAGGAGGAT 
GAGAACAGTG CTTTTGAATC TATGCCAGAT TCAAATCAAA GCCCAGAGCC TGAAAGAGAC 
CAGGCTGATG GTTCTAATCA ATGGTCTGAT GATACCTCTG GTCAAAGTGT TAATTCAGAA 
AGGACTCTTG GTGTCCTGGA ATCTCCTGTA GCTGGGCCAA GCAATAGAAC AGAAGGGGAT 
TGTACCTTAC CTAACACCAC TCCGTCTGTA ACTCAGCTAC CATCTCTGAG GGCAGATCAT 
CACCACTATC CAACTATTGA TGAACCTCTT CCACCAAACT GGGAAGCTCG TATTGACAGT 
CATGGAAGAG TTTTTTATGT AGACCATGTC AATCGAACCA CAACGTGGCA GCGTCCAACA 
ACAGCAGCAA CACCAGATGG TATAAGCAGA TCTGGATCTA TCCAACAGAT GGAGCAACTG 
AACCGCAGGT ATCAGAATAT CCAGAGGACT ATTGCAACAG AAAGAACAGA AGAAGATTCT 
TTAGGTCGTA ATGACAGGGC ACCCGGAGTT GGTGATTCGG AGACAGATCT GTCTCAGCCA 
AACACAGAGT TACGAAGAGA GGGATCCCTA ACACCCATAA ATTCCCAGAA AATTACGTTA 
CTGCTACAAT CCCCAGCTGT CAAGTTTATA ACCAACCCAG AGTTTTTTAC TATACTGCAT 
GCAAACTATA GTGCCTACCG AGTTTTTACA AACAGCACCT GCTTGAAGCA CATGGTTCTG 
AAAATCCGAA GGGATGCAAG GAACTTTGAA CGCTACCAGC ATAACAGGGA CTTGGTGAAC 
TTTATTAACA TGTTTGCCGA CACCCGGCTA GAGTTGCCAC GAGGATGGGA GATCAAAACT 
GACCAACAAG GAAAGTCTTT CTTTGTAGAC CACAACAGTC GAGCAACAAC ATTTATTGAC 
CCAAGAATTC CACTTCAAAA TGGGCGCCTT CCAAATCACC TGACACACAG ACAGCACCTT 
CAAAGGCTTA GAAGTTATAG CGCTGGAGAG GCTTCAGAAG TCTCCAGAAA TAGGGGAGCT 
TCTTTATTAG CCAGACCTGG CCATAGTTTA GTCACAGCAG TTCGAAACCA GCATCAACAT 
GAAGCTGTAC CTCTAGCCTA CAATGATAAG ATTGTTGCTT TTCTGCGTCA GCCGAATATT 
TTTGAGATGC TACAAGAACG TCAACCAAGT TTAGCCAGAA ATCATGCACT CAGGGAAAAG 
ATTCATTACA TACGAACAGA AGGGACTCAT GGATTGGAGA AATTATCATG TGATGCAGAT 
TTGGTAATAC TGTTAAGCCT TTTTGAAGAA GATATAATGT CATATATCCC TCTACAAGCT 
GCCTTCCATC CAGGATACAG CTTTTCCCCT CGCTGTTCAC CTGGCTCCTC ACCTCAGAAC 
TCTCCAGGGT TACAGCGTGC CAGTGCCAGG GCGCCCTCCC CATACAGAAG AGATTTTGAA 
GCCAAGTTAA GAAATTTCTA CAGAAAACTT GAAGCCAAAG GGTATGGTCA AGGCCCAGGA 
AAAATTAAAC TCATTATTCG CCGGGATCAT CTTTTGGAAG GAACCTTCAA CCAAGTAATG 
GCGTATTCCC GCAAAGAGCT CCAGAGGAAT AAGCTGTATA TCACATTTGT TGGAGAGGAA 
GGCCTGGATT ACAGCGGCCC ATCTAGAGAG TTCTTCTTTC TTCTCTCTCA GGAGCTCTTT 
AATCCCTACT ATGGGCTGTT TGAATATTCA GCAAATGATA CGTATACTGT ACAAATCAGC 
CCTATGTCAG CTTTTGTTGA GAACCACTTG GAATGGTTCC GGTTTAGTGG GAGAATCCTT 
GGACTTGCCC TTATTCATCA GTATCTGCTG GATGCCTTCT TTACAAGACC CTTTTATAAG 
GCCCTTCTAC GGCTTCCCTG TGACCTCAGT GACCTTGAAT ACCTAGATGA AGAATTTCAT 
CAGAGTTTAC AGTGGATGAA GGACAATGAT ATAACTGATA TCCTGGATCT TACATTCACT 
GTCAATGAGG AGGTGTTTGG GCAGGTCACT GAAAGAGAGC TCAAGTCTGG GGGTGCAAAT 
ATTCAGGTTT CAGAAAAAAA TAAGAAAGAA TATATTGAGA AAATGGTGAA ATGGCGAGTA 
GAGCGAGGAG TCGTACAGCA GACAGAAGCC CTTGTACGGG GATTTTATGA GGTGGTAGAT 
TCAAGGCTTG TATCTGTATT TGATGCAAGA GAACTTGAGC TGGTAATTGC AGGAACTGCT 
GAAATTGACC TCAATGACTG GCGCAATAAC ACAGAGTACC GAGGAGGATA TCATGATGGC 
CATATTGTCA TCCGGTGGTT CTGGGCAGCA GTGGAAAGGT TCAACAATGA ACAAAGGTTA 
CGACTCTTGC AGTTTGTCAC TGGGACATCT AGTGTGCCAT ATGAAGGGTT TGCAGCTCTG 
CGAGGAAGCA ACGGGCTGAG GCGATTCTGC ATAGAAAAAT GGGGGAAAAT AACATCTCTT 
CCAAGGGCAC ACACGTGCTT CAATCGCTTG GATCTTCCTC CATATCCTTC TTATTCAATG 
TTACATGAAA AATTGTTAAT AGCAGTCGAG GAGACAAGCA CCTTTGGACT TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950054.1
CDS	290..5086
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQNRRRCKEPMRYSYNPDQFHNMDIRNNPHEGVTMPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIVWKIEASSYFVESETKICFKYYHGVSGALRATTPSITVKNPTAPIFKNINNEETTQGQGSRRLISFTLSDFQALGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKIVCNTVNPVWKRELFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISVSSDTVPGTASASPLHNTNEETPIESQRRASVATECSSQELPNECISANGHDKNSASGSRSTSQNSLHKNITGSAMANASGAELTENLDSGQAEMTLPVDFIDSVQEQAACVDSEAKLENIVVSVAAAAAAGQNENPEILDVDEENMLLEASKDQEKAPEEPEGGCPLPDGEPKLRNTVKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSGDGDEEEQNSVDLDEESTVKETSEKDIVSETETVAPDPSSEVVIDENDDVENSRSPLPLDSTSPHLPSCNHNCLDVELLRTESESDSSARVHGDHECDTCDTSCYSTSCYSTSCYSTSCYSPSCYDSHNRFSSHTRFSSVDSARMSESTVFSSQDDEEDENSAFESMPDSNQSPEPERDQADGSNQWSDDTSGQSVNSERTLGVLESPVAGPSNRTEGDCTLPNTTPSVTQLPSLRADHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTTAATPDGISRSGSIQQMEQLNRRYQNIQRTIATERTEEDSLGRNDRAPGVGDSETDLSQPNTELRREGSLTPINSQKITLLLQSPAVKFITNPEFFTILHANYSAYRVFTNSTCLKHMVLKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVTAVRNQHQHEAVPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPGSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNDITDILDLTFTVNEEVFGQVTERELKSGGANIQVSEKNKKEYIEKMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLHEKLLIAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_018094565.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (hecw1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094565.2

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTTAAGAAT TTGTACCAAA ACAGGTTCCT TGGTTTGGCT 
GCCATGGCTT CTCCTTCAAG GAATTCTCAG AATAGGCGTC GATGCAAAGA ACCTATGAGG 
TATAGCTACA ATCCTGACCA GTTTCATAAT ATGGATATTA GGAACAACCC CCATGAAGGA 
GTAACCATGC CACGTTCCAC AAGTGACACT GACCTGGTAA CTTCTGATAG TCGGTCAACT 
CTGATGGTTA GTAGTTCTTA CTACTCAATA GGACACTCAC AGGATCTTGT AATCTACTGG 
GACATAAAAG AAGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTATCTAAT TGATGAAGTC 
TTATCAGAAA ACTTCTTGGA TTACAAGAAT CGTGGTGTGA ATGGATCTCA CAGAGGTCAG 
ATAGTCTGGA AGATTGAGGC CAGCTCTTAC TTTGTTGAGT CTGAAACAAA GATTTGTTTC 
AAATACTACC ATGGCGTAAG TGGAGCTCTA CGTGCTACAA CTCCCAGCAT CACTGTGAAA 
AATCCAACTG CCCCTATCTT TAAAAACATC AACAATGAAG AAACAACACA GGGGCAAGGA 
AGTCGCAGAC TCATAAGTTT TACCTTGTCA GATTTCCAAG CTCTGGGTTT AAAGAAAGGA 
ATGTTCTTCA ATCCGGATCC TTACCTGAAG ATTTCAATCC AGCCTGGAAA GCATAGTATC 
TTCCCAGCCC TGCCTCACCA TGGACAGGAG AAGAGATCAA AGATTGTATG CAACACTGTT 
AATCCTGTCT GGAAAAGAGA GCTTTTCAGT TTTGTCTCCC TTCCAACTGA CGTATTGGAA 
ATTGAAGTAA AGGATAAATT TGCCAAAAGC CGTCCAATCA TCAAACGATT TTTGGGAAAG 
CTCTCTATGC CAGTACAGCG CCTGCTGGAA AGGCATGCAA TTGGAGATCG TGTTGTGAGT 
TACACCTTAG GTCGCAGACT TCCTACAGAT CACGTCAGTG GACAACTGCA GTTCCGATTT 
GAAATAACAT CATCCATTCA CCCTGATGAT GAAGAGATCT CAGTGTCTTC AGACACAGTG 
CCAGGTACAG CTTCTGCCAG TCCACTTCAC AACACAAATG AGGAGACCCC TATTGAATCA 
CAGAGAAGAG CTTCGGTTGC AACTGAATGT TCCTCTCAAG AATTGCCAAA TGAATGTATT 
TCTGCAAATG GTCATGACAA GAACTCAGCA AGTGGCAGCA GGTCCACCAG TCAAAACAGC 
TTGCACAAAA ACATCACAGG AAGTGCCATG GCAAATGCGT CTGGAGCAGA ACTTACAGAA 
AACTTGGATT CTGGCCAAGC TGAGATGACT TTACCTGTTG ACTTCATAGA TTCTGTTCAG 
GAGCAAGCAG CGTGTGTTGA TTCTGAAGCC AAATTAGAGA ACATTGTAGT TTCTGTTGCT 
GCTGCTGCTG CTGCTGGGCA AAATGAAAAT CCAGAGATCC TGGATGTAGA TGAAGAAAAT 
ATGTTACTTG AAGCCAGTAA AGATCAAGAG AAAGCACCAG AGGAACCAGA GGGAGGGTGT 
CCCTTACCAG ATGGTGAACC AAAACTTAGA AATACGGTAA AAAGGAAAAA CAGGCCTTGT 
TCATTGCCAG TGTCAGAGCT GGAGACAGTC ATCGCATCAG CATGTGGAGA GCCTGAAACC 
CCTCGCACAC ATTACATTAG GATTCATAAT TTACTCCATA GTCTGCCTTC AGCACAGATG 
TCAGGTGATG GAGATGAGGA AGAGCAAAAC AGTGTTGATC TTGATGAAGA ATCCACAGTT 
AAGGAAACTT CAGAAAAAGA TATTGTTAGT GAAACTGAAA CTGTTGCTCC TGATCCTTCA 
TCTGAAGTTG TCATTGATGA AAATGATGAT GTTGAAAACA GCAGGAGCCC TTTACCTCTT 
GATAGCACGA GTCCTCATCT CCCTTCCTGT AACCATAATT GCCTGGATGT GGAGTTGCTT 
AGAACAGAAA GTGAAAGTGA CTCAAGTGCT AGGGTCCATG GAGACCACGA GTGTGACACT 
TGTGACACAT CTTGTTACAG CACATCCTGT TACAGCACAT CCTGCTACAG CACCTCATGT 
TATAGCCCCT CATGCTATGA TAGTCACAAC CGCTTTTCCA GTCACACCCG CTTCTCTTCA 
GTGGACAGTG CTAGAATGTC TGAAAGTACT GTCTTTTCTT CCCAGGATGA TGAGGAGGAT 
GAGAACAGTG CTTTTGAATC TATGCCAGAT TCAAATCAAA GCCCAGAGCC TGAAAGAGAC 
CAGGCTGATG GTTCTAATCA ATGGTCTGAT GATACCTCTG GTCAAAGTGT TAATTCAGAA 
AGGACTCTTG GTGTCCTGGA ATCTCCTGTA GCTGGGCCAA GCAATAGAAC AGAAGGGGAT 
TGTACCTTAC CTAACACCAC TCCGTCTGTA ACTCAGCTAC CATCTCTGAG GGCAGATCAT 
CACCACTATC CAACTATTGA TGAACCTCTT CCACCAAACT GGGAAGCTCG TATTGACAGT 
CATGGAAGAG TTTTTTATGT AGACCATGTC AATCGAACCA CAACGTGGCA GCGTCCAACA 
ACAGCAGCAA CACCAGATGG TATAAGCAGA TCTGGATCTA TCCAACAGAT GGAGCAACTG 
AACCGCAGGT ATCAGAATAT CCAGAGGACT ATTGCAACAG AAAGAACAGA AGAAGATTCT 
TTAGGTCGTA ATGACAGGGC ACCCGGAGTT GGTGATTCGG AGACAGATCT GTCTCAGCCA 
AACACAGAGT TACGAAGAGA GGGATCCCTA ACACCCATAA ATTCCCAGAA AATTACGTTA 
CTGCTACAAT CCCCAGCTGT CAAGTTTATA ACCAACCCAG AGTTTTTTAC TATACTGCAT 
GCAAACTATA GTGCCTACCG AGTTTTTACA AACAGCACCT GCTTGAAGCA CATGGTTCTG 
AAAATCCGAA GGGATGCAAG GAACTTTGAA CGCTACCAGC ATAACAGGGA CTTGGTGAAC 
TTTATTAACA TGTTTGCCGA CACCCGGCTA GAGTTGCCAC GAGGATGGGA GATCAAAACT 
GACCAACAAG GAAAGTCTTT CTTTGTAGAC CACAACAGTC GAGCAACAAC ATTTATTGAC 
CCAAGAATTC CACTTCAAAA TGGGCGCCTT CCAAATCACC TGACACACAG ACAGCACCTT 
CAAAGGCTTA GAAGTTATAG CGCTGGAGAG GCTTCAGAAG TCTCCAGAAA TAGGGGAGCT 
TCTTTATTAG CCAGACCTGG CCATAGTTTA GTCACAGCAG TTCGAAACCA GCATCAACAT 
GAAGCTGTAC CTCTAGCCTA CAATGATAAG ATTGTTGCTT TTCTGCGTCA GCCGAATATT 
TTTGAGATGC TACAAGAACG TCAACCAAGT TTAGCCAGAA ATCATGCACT CAGGGAAAAG 
ATTCATTACA TACGAACAGA AGGGACTCAT GGATTGGAGA AATTATCATG TGATGCAGAT 
TTGGTAATAC TGTTAAGCCT TTTTGAAGAA GATATAATGT CATATATCCC TCTACAAGCT 
GCCTTCCATC CAGGATACAG CTTTTCCCCT CGCTGTTCAC CTGGCTCCTC ACCTCAGAAC 
TCTCCAGGGT TACAGCGTGC CAGTGCCAGG GCGCCCTCCC CATACAGAAG AGATTTTGAA 
GCCAAGTTAA GAAATTTCTA CAGAAAACTT GAAGCCAAAG GGTATGGTCA AGGCCCAGGA 
AAAATTAAAC TCATTATTCG CCGGGATCAT CTTTTGGAAG GAACCTTCAA CCAAGTAATG 
GCGTATTCCC GCAAAGAGCT CCAGAGGAAT AAGCTGTATA TCACATTTGT TGGAGAGGAA 
GGCCTGGATT ACAGCGGCCC ATCTAGAGAG TTCTTCTTTC TTCTCTCTCA GGAGCTCTTT 
AATCCCTACT ATGGGCTGTT TGAATATTCA GCAAATGATA CGTATACTGT ACAAATCAGC 
CCTATGTCAG CTTTTGTTGA GAACCACTTG GAATGGTTCC GGTTTAGTGG GAGAATCCTT 
GGACTTGCCC TTATTCATCA GTATCTGCTG GATGCCTTCT TTACAAGACC CTTTTATAAG 
GCCCTTCTAC GGCTTCCCTG TGACCTCAGT GACCTTGAAT ACCTAGATGA AGAATTTCAT 
CAGAGTTTAC AGTGGATGAA GGACAATGAT ATAACTGATA TCCTGGATCT TACATTCACT 
GTCAATGAGG AGGTGTTTGG GCAGGTCACT GAAAGAGAGC TCAAGTCTGG GGGTGCAAAT 
ATTCAGGTTT CAGAAAAAAA TAAGAAAGAA TATATTGAGA AAATGGTGAA ATGGCGAGTA 
GAGCGAGGAG TCGTACAGCA GACAGAAGCC CTTGTACGGG GATTTTATGA GGTGGTAGAT 
TCAAGGCTTG TATCTGTATT TGATGCAAGA GAACTTGAGC TGGTAATTGC AGGAACTGCT 
GAAATTGACC TCAATGACTG GCGCAATAAC ACAGAGTACC GAGGAGGATA TCATGATGGC 
CATATTGTCA TCCGGTGGTT CTGGGCAGCA GTGGAAAGGT TCAACAATGA ACAAAGGTTA 
CGACTCTTGC AGTTTGTCAC TGGGACATCT AGTGTGCCAT ATGAAGGGTT TGCAGCTCTG 
CGAGGAAGCA ACGGGCTGAG GCGATTCTGC ATAGAAAAAT GGGGGAAAAT AACATCTCTT 
CCAAGGGCAC ACACGTGCTT CAATCGCTTG GATCTTCCTC CATATCCTTC TTATTCAATG 
TTACATGAAA AATTGTTAAT AGCAGTCGAG GAGACAAGCA CCTTTGGACT TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hecw1 ( NM_001130278.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001123750.1 Xenopus tropicalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (hecw1), mRNA.

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CloneID	OXa22181
Clone ID Related Accession (Same CDS sequence)	NM_001130278.1
Accession Version	NM_001130278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4734bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase HECW1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC167464.1. ##Evidence-Data-START## Transcript exon combination :: BC167464.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGCTTCTC CTTCAAGGAA TTCTCAGAAT AGGCGTCGAT GCAAAGAACC TATGAGGTAT 
AGCTACAATC CTGACCAGTT TCATAATATG GATATTAGGA ACAACCCCCA TGAAGGAGTA 
ACCATGCCAC GTTCCACAAG TGACACTGAC CTGGTAACTT CTGATAGTCG GTCAACTCTG 
ATGGTTAGTA GTTCTTACTA CTCAATAGGA CACTCACAGG ATCTTGTAAT CTACTGGGAC 
ATAAAAGAAG AAGTGGATGC TGGAGACTGG ATTGGCATGT ATCTAATTGA TGAAGTCTTA 
TCAGAAAACT TCTTGGATTA CAAGAATCGT GGTGTGAATG GATCTCACAG AGGTCAGATA 
GTCTGGAAGA TTGAGGCCAG CTCTTACTTT GTTGAGTCTG AAACAAAGAT TTGTTTCAAA 
TACTACCATG GCGTAAGTGG AGCTCTACGT GCTACAACTC CCAGCATCAC TGTGAAAAAT 
CCAACTGCCC CTATCTTTAA AAACATCAAC AATGAAGAAA CAACACAGGG GCAAGGAAGT 
CGCAGACTCA TAAGTTTTAC CTTGTCAGAT TTCCAAGCTC TGGGTTTAAA GAAAGGAATG 
TTCTTCAATC CGGATCCTTA CCTGAAGATT TCAATCCAGC CTGGAAAGCA TAGTATCTTC 
CCAGCCCTGC CTCACCATGG ACAGGAGAAG AGATCAAAGA TTGTATGCAA CACTGTTAAT 
CCTGTCTGGA AAAGAGAGCT TTTCAGTTTT GTCTCCCTTC CAACTGACGT ATTGGAAATT 
GAAGTAAAGG ATAAATTTGC CAAAAGCCGT CCAATCATCA AACGATTTTT GGGAAAGCTC 
TCTATGCCAG TACAGCGCCT GCTGGAAAGG CATGCAATTG GAGATCGTGT TGTGAGTTAC 
ACCTTAGGTC GCAGACTTCC TACAGATCAC GTCAGTGGAC AACTGCAGTT CCGATTTGAA 
ATAACATCAT CCATTCACCC TGATGATGAA GAGATCTCAG TGTCTTCAGA CACAGTGCCA 
GGTACAGCTT CTGCCAGTCC ACTTCACAAC ACAAATGAGG AGACCCCTAT TGAATCACAG 
AGAAGAGCTT CGGTTGCAAC TGAATGTTCC TCTCAAGAAT TGCCAAATGA ATGTATTTCT 
GCAAATGGTC ATGACAAGAA CTCAGCAAGT GGCAGCAGGT CCACCAGTCA AAACAGCTTG 
CACAAAAACA TCACAGGAAG TGCCATGGCA AATGCGTCTG GAGCAGAACT TACAGAAAAC 
TTGGATTCTG GCCAAGCTGA GATGACTTTA CCTGTTGACT TCATAGATTC TGTTCAGGAG 
CAAGCAGCGT GTGTTGATTC TGAAGCCAAA TTAGAGAACA TTGTAGTTTC TGTTGCTGCT 
GCTGCTGCTG CTGGGCAAAA TGAAAATCCA GAGATCCTGG ATGTAGATGA AGAAAATATG 
TTACTTGAAG CCAGTAAAGA TCAAGAGAAA GCACCAGAGG AACCAGAGGG AGGGTGTCCC 
TTACCAGATG GTGAACCAAA ACTTAGAAAT ACGGTAAAAA GGAAAAACAG GCCTTGTTCA 
TTGCCAGTGT CAGAGCTGGA GACAGTCATC GCATCAGCAT GTGGAGAGCC TGAAACCCCT 
CGCACACATT ACATTAGGAT TCATAATTTA CTCCATAGTC TGCCTTCAGC ACAGATGTCA 
GGTGATGGAG ATGAGGAAGA GCAAAACAGT GTTGATCTTG ATGAAGAATC CACAGTTAAG 
GAAACTTCAG AAAAAGATAT TGTTAGTGAA ACTGAAACTG TTGCTCCTGA TCCTTCATCT 
GAAGTTGTCA TTGATGAAAA TGATGATGTT GAAAACAGCA GGAGCCCTTT ACCTCTTGAT 
AGCACGAGTC CTCATCTCCC TTCCTGTAAC CATAATTGCC TGGATGTGGA GTTGCTTAGA 
ACAGAAAGTG AAAGTGACTC AAGTGCTAGG GTCCATGGAG ACCACGAGTG TGACACTTGT 
GACACATCTT GTTACAGCAC ATCCTGTTAC AGCACATCCT GCTACAGCAC CTCATGTTAT 
AGCCCCTCAT GCTATGATAG TCACAACCGC TTTTCCAGTC ACACCCGCTT CTCTTCAGTG 
GACAGTGCTA GAATGTCTGA AAGTACTGTC TTTTCTTCCC AGGATGATGA GGAGGATGAG 
AACAGTGCTT TTGAATCTAT GCCAGATTCA AATCAAAGCC CAGAGCCTGA AAGAGACCAG 
GCTGATGGTT CTAATCAATG GTCTGATGAT ACCTCTGGTC AAAGTGTTAA TTCAGAAAGG 
ACTCTTGGTG TCCTGGAATC TCCTGTAGCT GGGCCAAGCA ATAGAACAGA AGGGGATTGT 
ACCTTACCTA ACACCACTCC GTCTGTAACT CAGCTACCAT CTCTGAGGGC AGATCATCAC 
CACTATCCAA CTATTGATGA ACCTCTTCCA CCAAACTGGG AAGCTCGTAT TGACAGTCAT 
GGAAGAGTTT TTTATGTAGA CCATGTCAAT CGAACCACAA CGTGGCAGCG TCCAACAACA 
GCAGCAACAC CAGATGGTAT AAGCAGATCT GGATCTATCC AACAGATGGA GCAACTGAAC 
CGCAGGTATC AGAATATCCA GAGGACTATT GCAACAGAAA GAACAGAAGA AGATTCTTTA 
GGTCGTAATG ACAGGGCACC CGGAGTTGGT GATTCGGAGA CAGATCTGTC TCAGCCAAAC 
ACAGAGTTAC GAAGAGAGGG ATCCCTAACA CCCATAAATT CCCAGAAAAT TACGTTACTG 
CTACAATCCC CAGCTGTCAA GTTTATAACC AACCCAGAGT TTTTTACTAT ACTGCATGCA 
AACTATAGTG CCTACCGAGT TTTTACAAAC AGCACCTGCT TGAAGCACAT GGTTCTGAAA 
ATCCGAAGGG ATGCAAGGAA CTTTGAACGC TACCAGCATA ACAGGGACTT GGTGAACTTT 
ATTAACATGT TTGCCGACAC CCGGCTAGAG TTGCCACGAG GATGGGAGAT CAAAACTGAC 
CAACAAGGAA AGTCTTTCTT TGTAGACCAC AACAGTCGAG CAACAACATT TATTGACCCA 
AGAATTCCAC TTCAAAATGG GCGCCTTCCA AATCACCTGA CACACAGACA GCACCTTCAA 
AGGCTTAGAA GTTATAGCGC TGGAGAGGCT TCAGAAGTCT CCAGAAATAG GGGAGCTTCT 
TTATTAGCCA GACCTGGCCA TAGTTTAGTC ACAGCAGTTC GAAACCAGCA TCAACATGAA 
GCTGTACCTC TAGCCTACAA TGATAAGATT GTTGCTTTTC TGCGTCAGCC GAATATTTTT 
GAGATGCTAC AAGAACGTCA ACCAAGTTTA GCCAGAAATC ATGCACTCAG GGAAAAGATT 
CATTACATAC GAACAGAAGG GACTCATGGA TTGGAGAAAT TATCATGTGA TGCAGATTTG 
GTAATACTGT TAAGCCTTTT TGAAGAAGAT ATAATGTCAT ATATCCCTCT ACAAGCTGCC 
TTCCATCCAG GATACAGCTT TTCCCCTCGC TGTTCACCTG GCTCCTCACC TCAGAACTCT 
CCAGGGTTAC AGCGTGCCAG TGCCAGGGCG CCCTCCCCAT ACAGAAGAGA TTTTGAAGCC 
AAGTTAAGAA ATTTCTACAG AAAACTTGAA GCCAAAGGGT ATGGTCAAGG CCCAGGAAAA 
ATTAAACTCA TTATTCGCCG GGATCATCTT TTGGAAGGAA CCTTCAACCA AGTAATGGCG 
TATTCCCGCA AAGAGCTCCA GAGGAATAAG CTGTATATCA CATTTGTTGG AGAGGAAGGC 
CTGGATTACA GCGGCCCATC TAGAGAGTTC TTCTTTCTTC TCTCTCAGGA GCTCTTTAAT 
CCCTACTATG GGCTGTTTGA ATATTCAGCA AATGATACGT ATACTGTACA AATCAGCCCT 
ATGTCAGCTT TTGTTGAGAA CCACTTGGAA TGGTTCCGGT TTAGTGGGAG AATCCTTGGA 
CTTGCCCTTA TTCATCAGTA TCTGCTGGAT GCCTTCTTTA CAAGACCCTT TTATAAGGCC 
CTTCTACGGC TTCCCTGTGA CCTCAGTGAC CTTGAATACC TAGATGAAGA ATTTCATCAG 
AGTTTACAGT GGATGAAGGA CAATGATATA ACTGATATCC TGGATCTTAC ATTCACTGTC 
AATGAGGAGG TGTTTGGGCA GGTCACTGAA AGAGAGCTCA AGTCTGGGGG TGCAAATATT 
CAGGTTTCAG AAAAAAATAA GAAAGAATAT ATTGAGAAAA TGGTGAAATG GCGAGTAGAG 
CGAGGAGTCG TACAGCAGAC AGAAGCCCTT GTACGGGGAT TTTATGAGGT GGTAGATTCA 
AGGCTTGTAT CTGTATTTGA TGCAAGAGAA CTTGAGCTGG TAATTGCAGG AACTGCTGAA 
ATTGACCTCA ATGACTGGCG CAATAACACA GAGTACCGAG GAGGATATCA TGATGGCCAT 
ATTGTCATCC GGTGGTTCTG GGCAGCAGTG GAAAGGTTCA ACAATGAACA AAGGTTACGA 
CTCTTGCAGT TTGTCACTGG GACATCTAGT GTGCCATATG AAGGGTTTGC AGCTCTGCGA 
GGAAGCAACG GGCTGAGGCG ATTCTGCATA GAAAAATGGG GGAAAATAAC ATCTCTTCCA 
AGGGCACACA CGTGCTTCAA TCGCTTGGAT CTTCCTCCAT ATCCTTCTTA TTCAATGTTA 
CATGAAAAAT TGTTAATAGC AGTCGAGGAG ACAAGCACCT TTGGACTTGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123750.1
CDS	206..4939
Translation	MASPSRNSQNRRRCKEPMRYSYNPDQFHNMDIRNNPHEGVTMPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIYWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIVWKIEASSYFVESETKICFKYYHGVSGALRATTPSITVKNPTAPIFKNINNEETTQGQGSRRLISFTLSDFQALGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQEKRSKIVCNTVNPVWKRELFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISVSSDTVPGTASASPLHNTNEETPIESQRRASVATECSSQELPNECISANGHDKNSASGSRSTSQNSLHKNITGSAMANASGAELTENLDSGQAEMTLPVDFIDSVQEQAACVDSEAKLENIVVSVAAAAAAGQNENPEILDVDEENMLLEASKDQEKAPEEPEGGCPLPDGEPKLRNTVKRKNRPCSLPVSELETVIASACGEPETPRTHYIRIHNLLHSLPSAQMSGDGDEEEQNSVDLDEESTVKETSEKDIVSETETVAPDPSSEVVIDENDDVENSRSPLPLDSTSPHLPSCNHNCLDVELLRTESESDSSARVHGDHECDTCDTSCYSTSCYSTSCYSTSCYSPSCYDSHNRFSSHTRFSSVDSARMSESTVFSSQDDEEDENSAFESMPDSNQSPEPERDQADGSNQWSDDTSGQSVNSERTLGVLESPVAGPSNRTEGDCTLPNTTPSVTQLPSLRADHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTTAATPDGISRSGSIQQMEQLNRRYQNIQRTIATERTEEDSLGRNDRAPGVGDSETDLSQPNTELRREGSLTPINSQKITLLLQSPAVKFITNPEFFTILHANYSAYRVFTNSTCLKHMVLKIRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVTAVRNQHQHEAVPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHALREKIHYIRTEGTHGLEKLSCDADLVILLSLFEEDIMSYIPLQAAFHPGYSFSPRCSPGSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNDITDILDLTFTVNEEVFGQVTERELKSGGANIQVSEKNKKEYIEKMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLHEKLLIAVEETSTFGLE

Target ORF information:

RefSeq Version	NM_001130278.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (hecw1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130278.1

ORF Insert Sequence:

ATGGCTTCTC CTTCAAGGAA TTCTCAGAAT AGGCGTCGAT GCAAAGAACC TATGAGGTAT 
AGCTACAATC CTGACCAGTT TCATAATATG GATATTAGGA ACAACCCCCA TGAAGGAGTA 
ACCATGCCAC GTTCCACAAG TGACACTGAC CTGGTAACTT CTGATAGTCG GTCAACTCTG 
ATGGTTAGTA GTTCTTACTA CTCAATAGGA CACTCACAGG ATCTTGTAAT CTACTGGGAC 
ATAAAAGAAG AAGTGGATGC TGGAGACTGG ATTGGCATGT ATCTAATTGA TGAAGTCTTA 
TCAGAAAACT TCTTGGATTA CAAGAATCGT GGTGTGAATG GATCTCACAG AGGTCAGATA 
GTCTGGAAGA TTGAGGCCAG CTCTTACTTT GTTGAGTCTG AAACAAAGAT TTGTTTCAAA 
TACTACCATG GCGTAAGTGG AGCTCTACGT GCTACAACTC CCAGCATCAC TGTGAAAAAT 
CCAACTGCCC CTATCTTTAA AAACATCAAC AATGAAGAAA CAACACAGGG GCAAGGAAGT 
CGCAGACTCA TAAGTTTTAC CTTGTCAGAT TTCCAAGCTC TGGGTTTAAA GAAAGGAATG 
TTCTTCAATC CGGATCCTTA CCTGAAGATT TCAATCCAGC CTGGAAAGCA TAGTATCTTC 
CCAGCCCTGC CTCACCATGG ACAGGAGAAG AGATCAAAGA TTGTATGCAA CACTGTTAAT 
CCTGTCTGGA AAAGAGAGCT TTTCAGTTTT GTCTCCCTTC CAACTGACGT ATTGGAAATT 
GAAGTAAAGG ATAAATTTGC CAAAAGCCGT CCAATCATCA AACGATTTTT GGGAAAGCTC 
TCTATGCCAG TACAGCGCCT GCTGGAAAGG CATGCAATTG GAGATCGTGT TGTGAGTTAC 
ACCTTAGGTC GCAGACTTCC TACAGATCAC GTCAGTGGAC AACTGCAGTT CCGATTTGAA 
ATAACATCAT CCATTCACCC TGATGATGAA GAGATCTCAG TGTCTTCAGA CACAGTGCCA 
GGTACAGCTT CTGCCAGTCC ACTTCACAAC ACAAATGAGG AGACCCCTAT TGAATCACAG 
AGAAGAGCTT CGGTTGCAAC TGAATGTTCC TCTCAAGAAT TGCCAAATGA ATGTATTTCT 
GCAAATGGTC ATGACAAGAA CTCAGCAAGT GGCAGCAGGT CCACCAGTCA AAACAGCTTG 
CACAAAAACA TCACAGGAAG TGCCATGGCA AATGCGTCTG GAGCAGAACT TACAGAAAAC 
TTGGATTCTG GCCAAGCTGA GATGACTTTA CCTGTTGACT TCATAGATTC TGTTCAGGAG 
CAAGCAGCGT GTGTTGATTC TGAAGCCAAA TTAGAGAACA TTGTAGTTTC TGTTGCTGCT 
GCTGCTGCTG CTGGGCAAAA TGAAAATCCA GAGATCCTGG ATGTAGATGA AGAAAATATG 
TTACTTGAAG CCAGTAAAGA TCAAGAGAAA GCACCAGAGG AACCAGAGGG AGGGTGTCCC 
TTACCAGATG GTGAACCAAA ACTTAGAAAT ACGGTAAAAA GGAAAAACAG GCCTTGTTCA 
TTGCCAGTGT CAGAGCTGGA GACAGTCATC GCATCAGCAT GTGGAGAGCC TGAAACCCCT 
CGCACACATT ACATTAGGAT TCATAATTTA CTCCATAGTC TGCCTTCAGC ACAGATGTCA 
GGTGATGGAG ATGAGGAAGA GCAAAACAGT GTTGATCTTG ATGAAGAATC CACAGTTAAG 
GAAACTTCAG AAAAAGATAT TGTTAGTGAA ACTGAAACTG TTGCTCCTGA TCCTTCATCT 
GAAGTTGTCA TTGATGAAAA TGATGATGTT GAAAACAGCA GGAGCCCTTT ACCTCTTGAT 
AGCACGAGTC CTCATCTCCC TTCCTGTAAC CATAATTGCC TGGATGTGGA GTTGCTTAGA 
ACAGAAAGTG AAAGTGACTC AAGTGCTAGG GTCCATGGAG ACCACGAGTG TGACACTTGT 
GACACATCTT GTTACAGCAC ATCCTGTTAC AGCACATCCT GCTACAGCAC CTCATGTTAT 
AGCCCCTCAT GCTATGATAG TCACAACCGC TTTTCCAGTC ACACCCGCTT CTCTTCAGTG 
GACAGTGCTA GAATGTCTGA AAGTACTGTC TTTTCTTCCC AGGATGATGA GGAGGATGAG 
AACAGTGCTT TTGAATCTAT GCCAGATTCA AATCAAAGCC CAGAGCCTGA AAGAGACCAG 
GCTGATGGTT CTAATCAATG GTCTGATGAT ACCTCTGGTC AAAGTGTTAA TTCAGAAAGG 
ACTCTTGGTG TCCTGGAATC TCCTGTAGCT GGGCCAAGCA ATAGAACAGA AGGGGATTGT 
ACCTTACCTA ACACCACTCC GTCTGTAACT CAGCTACCAT CTCTGAGGGC AGATCATCAC 
CACTATCCAA CTATTGATGA ACCTCTTCCA CCAAACTGGG AAGCTCGTAT TGACAGTCAT 
GGAAGAGTTT TTTATGTAGA CCATGTCAAT CGAACCACAA CGTGGCAGCG TCCAACAACA 
GCAGCAACAC CAGATGGTAT AAGCAGATCT GGATCTATCC AACAGATGGA GCAACTGAAC 
CGCAGGTATC AGAATATCCA GAGGACTATT GCAACAGAAA GAACAGAAGA AGATTCTTTA 
GGTCGTAATG ACAGGGCACC CGGAGTTGGT GATTCGGAGA CAGATCTGTC TCAGCCAAAC 
ACAGAGTTAC GAAGAGAGGG ATCCCTAACA CCCATAAATT CCCAGAAAAT TACGTTACTG 
CTACAATCCC CAGCTGTCAA GTTTATAACC AACCCAGAGT TTTTTACTAT ACTGCATGCA 
AACTATAGTG CCTACCGAGT TTTTACAAAC AGCACCTGCT TGAAGCACAT GGTTCTGAAA 
ATCCGAAGGG ATGCAAGGAA CTTTGAACGC TACCAGCATA ACAGGGACTT GGTGAACTTT 
ATTAACATGT TTGCCGACAC CCGGCTAGAG TTGCCACGAG GATGGGAGAT CAAAACTGAC 
CAACAAGGAA AGTCTTTCTT TGTAGACCAC AACAGTCGAG CAACAACATT TATTGACCCA 
AGAATTCCAC TTCAAAATGG GCGCCTTCCA AATCACCTGA CACACAGACA GCACCTTCAA 
AGGCTTAGAA GTTATAGCGC TGGAGAGGCT TCAGAAGTCT CCAGAAATAG GGGAGCTTCT 
TTATTAGCCA GACCTGGCCA TAGTTTAGTC ACAGCAGTTC GAAACCAGCA TCAACATGAA 
GCTGTACCTC TAGCCTACAA TGATAAGATT GTTGCTTTTC TGCGTCAGCC GAATATTTTT 
GAGATGCTAC AAGAACGTCA ACCAAGTTTA GCCAGAAATC ATGCACTCAG GGAAAAGATT 
CATTACATAC GAACAGAAGG GACTCATGGA TTGGAGAAAT TATCATGTGA TGCAGATTTG 
GTAATACTGT TAAGCCTTTT TGAAGAAGAT ATAATGTCAT ATATCCCTCT ACAAGCTGCC 
TTCCATCCAG GATACAGCTT TTCCCCTCGC TGTTCACCTG GCTCCTCACC TCAGAACTCT 
CCAGGGTTAC AGCGTGCCAG TGCCAGGGCG CCCTCCCCAT ACAGAAGAGA TTTTGAAGCC 
AAGTTAAGAA ATTTCTACAG AAAACTTGAA GCCAAAGGGT ATGGTCAAGG CCCAGGAAAA 
ATTAAACTCA TTATTCGCCG GGATCATCTT TTGGAAGGAA CCTTCAACCA AGTAATGGCG 
TATTCCCGCA AAGAGCTCCA GAGGAATAAG CTGTATATCA CATTTGTTGG AGAGGAAGGC 
CTGGATTACA GCGGCCCATC TAGAGAGTTC TTCTTTCTTC TCTCTCAGGA GCTCTTTAAT 
CCCTACTATG GGCTGTTTGA ATATTCAGCA AATGATACGT ATACTGTACA AATCAGCCCT 
ATGTCAGCTT TTGTTGAGAA CCACTTGGAA TGGTTCCGGT TTAGTGGGAG AATCCTTGGA 
CTTGCCCTTA TTCATCAGTA TCTGCTGGAT GCCTTCTTTA CAAGACCCTT TTATAAGGCC 
CTTCTACGGC TTCCCTGTGA CCTCAGTGAC CTTGAATACC TAGATGAAGA ATTTCATCAG 
AGTTTACAGT GGATGAAGGA CAATGATATA ACTGATATCC TGGATCTTAC ATTCACTGTC 
AATGAGGAGG TGTTTGGGCA GGTCACTGAA AGAGAGCTCA AGTCTGGGGG TGCAAATATT 
CAGGTTTCAG AAAAAAATAA GAAAGAATAT ATTGAGAAAA TGGTGAAATG GCGAGTAGAG 
CGAGGAGTCG TACAGCAGAC AGAAGCCCTT GTACGGGGAT TTTATGAGGT GGTAGATTCA 
AGGCTTGTAT CTGTATTTGA TGCAAGAGAA CTTGAGCTGG TAATTGCAGG AACTGCTGAA 
ATTGACCTCA ATGACTGGCG CAATAACACA GAGTACCGAG GAGGATATCA TGATGGCCAT 
ATTGTCATCC GGTGGTTCTG GGCAGCAGTG GAAAGGTTCA ACAATGAACA AAGGTTACGA 
CTCTTGCAGT TTGTCACTGG GACATCTAGT GTGCCATATG AAGGGTTTGC AGCTCTGCGA 
GGAAGCAACG GGCTGAGGCG ATTCTGCATA GAAAAATGGG GGAAAATAAC ATCTCTTCCA 
AGGGCACACA CGTGCTTCAA TCGCTTGGAT CTTCCTCCAT ATCCTTCTTA TTCAATGTTA 
CATGAAAAAT TGTTAATAGC AGTCGAGGAG ACAAGCACCT TTGGACTTGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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hecw1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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