Hecw1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hecw1
Entrez Gene ID	291209
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Synonyms	RGD1561038
General protein information	Preferred Names HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase HECW1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017600487.1	XP_017455976.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_017600488.1	XP_017455977.1	E3 ubiquitin-protein ligase HECW1 isoform X4
XM_017600485.1	XP_017455974.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_017600489.1	XP_017455978.1	E3 ubiquitin-protein ligase HECW1 isoform X5
XM_008771697.2	XP_008769919.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_017600486.1	XP_017455975.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_017600490.1	XP_017455979.1	E3 ubiquitin-protein ligase HECW1 isoform X6
NM_001106117.1	NP_001099587.1	E3 ubiquitin-protein ligase HECW1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HECW1	NP_055867.3
Pan troglodytes (chimpanzee)	HECW1	XP_003318432.1
Canis lupus familiaris (dog)	HECW1	XP_005630981.1
Danio rerio (zebrafish)	hecw1b	NP_001139236.1
Mus musculus (house mouse)	Hecw1	NP_001074817.3
Xenopus tropicalis (tropical clawed frog)	hecw1	NP_001123750.1
	HECW1	XP_001096775.1
Rattus norvegicus (Norway rat)	Hecw1	NP_001099587.1
Gallus gallus (chicken)	HECW1	XP_004939648.1

Gene Ontology
Bibliography

Related articles in PubMed

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Gaudet P, Livstone MS, Lewis SE, Thomas PD
Briefings in bioinformatics12(5)449-62(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Hecw1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hecw1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa41415	NM_001106117.1 Latest version!	Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa44901	XM_017600486.1 Latest version!	Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa44901	XM_008771697.2 Latest version!	Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa59015	XM_017600485.1 Latest version!	Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa59016	XM_017600487.1 Latest version!	Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa59017	XM_017600488.1 Latest version!	Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa59018	XM_017600489.1 Latest version!	Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa59019	XM_017600490.1 Latest version!	Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hecw1 ( NM_001106117.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099587.1 Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), mRNA.

Price & Availability↑

CloneID	ORa41415
Clone ID Related Accession (Same CDS sequence)	NM_001106117.1
Accession Version	NM_001106117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase HECW1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474030.1. On or before Oct 4, 2007 this sequence version replaced XM_001054379.1, XM_225450.4.

ATGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCTGCCA TGGCATCTCC ATCTAGAAAC 
TCCCAGAGCC GACGCCGGTG CAAAGAGCCC CTCCGGTACA GCTATAACCC AGACCAATTC 
CACAACATAG ACATCAGAAA TGGTGCCCAT GATGGCATCA CCATCCCTCG CTCTACCAGT 
GACACTGACC TGGTCACCTC AGACAGCCGC TCTACACTCA TGGTCAGCAG TTCCTACTAT 
TCTATAGGGT ATTCCCAGGA TTTGGTGATC CACTGGGACA TCAAGGAAGA AGTGGATGCC 
GGAGACTGGA TTGGGATGTA CCTTATTGGT GAAGTCTCAT CTGAAAACTT TCTGGACTAT 
AAGAACCGTG GAGTCAATGG ATCTCACAGA GGCCAGATCA TTTGGAAGAT TGATGCCAGC 
TCTTACTTTG TGGAATCTGA AACTAAGATC TGCTTCAAAT ACTACCACGG AGTAAGCGGG 
GCCCTGAGAG CTACAACCCC CAGTGTCACT GTCAAGAACT CAGCGGCTCC TATTTTTAAA 
GGTATTGGCT CTGAGGAGAC TGTGCAAAGT CAAGGAAACA GGAGGCTCAT CAGCTTCTCT 
CTCTCAGATT TCCAGGCCAT GGGATTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAT 
CTGAAGATTT CCATTCAACC TGGTAAACAT AGCATCTTCC CGGCCCTCCC TCATCATGGA 
CAGGAACGGA GATCCACGAT CATAGGCAAC ACTGTAAACC CCATCTGGCA GGCAGAGCAT 
TTCAGTTTCG TGTCTCTGCC AACTGATGTG CTGGAAATTG AAGTGAAGGA CAAGTTTGCC 
AAGAGTCGTC CCATCATCAA GCGTTTCTTG GGAAAGCTGT CAATGCCAGT TCAGAGGCTA 
CTGGAGAGAC ATGCCATAGG GGATAGGGTG GTCAGCTACA CACTTGGCCG CAGGCTTCCA 
ACTGACCATG TGAGTGGACA TCTGCAATTC CGATTTGAGA TCACATCCTC CATCCACGCA 
GACGATGAAG AGATCTCTCT GAATGCTGAG CCTGAGTCAG CGGAAGTGCA GGACAGTGTC 
ATGAATAGTG TGGTGGAAAA CAGCAGTGGG GAGCCTCCCG GTGATGCTGC AGAATTCCTC 
AAGGACTTGA AGCCAGAGGC ACCGAGTGAA GAGGATGGAG TGCGCGGCTC AGAAAATGAA 
AACCGAGAGC ATGCCAGGCC AGTCGAGGAA GCTGCGGGTG CTATGGAGAC CAGAGATGAT 
AGCAAGTTCT CTGAAGGACC GGAAGAGGTT GGAGAGCTTC AGGACCCAGA GCAACATGAC 
ACCCAGCCCA CTCTGAGCGC AGAAGAAGTG GCAGAGCGAC TTCCCCTGGG TGAGGGTTGC 
CCACCCTCGC TGTTGCCAGA AGAGAGTACA GCTCTAGGCA GCAAGGCAGA AGAGGAAGCA 
ATTCCAGAAA ATGGAGCGGG AGAGGAAGAG ATGCAGGAGA AGGGGAAAGA TGAGGAGGAA 
GAAGAGGGTG TTTCTGCCCT GGAGCAAGGA GAGCCTGGAC TTGAGCTGAG GGCCTCCGTG 
AGAAAAAAAA GCAGGCCATG TTCCTTACCT GTATCCGAGC TTGAAACAGT GATCGCATCT 
GCCTGTGGAG AGGCTGAGAC CCCAAGGACC CACTACATCC GCATCCACAC CCTACTGCAC 
AGCATGCCCT CGGCCCAGAG GGGAAGCACC ACAGAGGAGG AGGATGCCAT GGAGGAGGAA 
TCCACCCTCA AGGAGTCCTC TGAAAAGGAT GGGCTCAGCG AGGTGGACAC AATAGCTGCT 
GACCCACAGT CCATGGAAGA TGGCGAGAAC AATGGCGCTA CTCTCTGCAT GGCATCCTCT 
GACTGCTCTG GGGGCCATTT CTCCAATTTA TCTAAAAGCA TTGGCGCTGG CCAAGATGGC 
GAAGCTCACC CCAGCACTGG GAGTGAGAGT GACTCCAGCC CCCAGCAGGG AGCAGACCAC 
AGTTGCGAGG GCTGTGATGC CTCATGCTGT AGCCCCTCAT GCTATAGCAC ATCGTGCTAC 
AGCAGCTCCT GTTATAGCAG CTCCTGTTAC AGCAGCTCCT GCTACAATGG CAACAATCGG 
TTTGCCAGCC ACACACGCTT CTCCTCGGTG GACAGCGCCA AGATCTCTGA GAGCACAGTC 
TTTTCTTCAC AAGAGGATGA GGAGGAGGAA AACAGTGCAT TCGAGTCGGT ACCTGACTCG 
GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT ACAAATGGGG CTGGGCCTTG GCAGGATGAA 
CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA ACTACTGAAG GTCTGGACTC CCCCATGGCA 
GGCCCTAGCA ATAGGAGAGA AGGTGACTGT CCTATGCTCC ATAATTCCCA GCCAATAAGC 
CAGCTTCCTT CCTTGAGGCC TGAACATCAT CACTACCCAA CAATCGATGA GCCTCTTCCA 
CCAAACTGGG AAGCTCGAAT CGACAGCCAT GGACGGGTCT TTTATGTGGA CCATGTAAAT 
CGCACAACCA CCTGGCAGCG ACCATCAATG GCGCCTACCC CAGATGGAAT GATCAGATCC 
GGGTCTGTCC ACCAGATGGA GCAGCTTAAC AGAAGGTATC AAAACATCCA GAGAACCATT 
GCAACAGAGA GATCTGAAGA AGATTCTGGC AACCAAAGCT GTGAGCAAAT ACCAGACGGA 
GGAGCTGGAG GTGGAGGGAG TGACTCGGAA GCAGAATCTT CCCAATCAAA CTTAGATCTG 
AGGAGAGAAG GGTCACTTTC TCCTGTCAAC TCACAGAAGG TCACCTTGCT GCTGCAGTCT 
CCAGCAGTCA AGTTCATCAC CAATCCTGAG TTCTTCACTG TGTTGCATGC CAATTATAGT 
GCCTACAGGG TTTTTACAAG TAGCACTTGC CTGAAGCATA TGATTTTGAA AGTCCGAAGA 
GATGCTCGCA ATTTTGAACG CTACCAGCAC AACCGGGACC TGGTGAATTT CATCAACATG 
TTTGCAGATA CTCGGCTGGA ACTGCCCCGG GGTTGGGAGA TCAAAACTGA TCATCAGGGA 
AAGTCTTTCT TTGTGGACCA CAACAGTCGG GCTACTACTT TCATTGATCC CCGAATCCCT 
CTTCAGAATG GCCGCCTTCC CAATCATCTA ACCCACCGAC AGCACCTCCA GAGGCTCAGG 
AGTTACAGTG CTGGTGAGGC CTCAGAAGTC TCTAGAAACA GAGGAGCATC TTTGCTGGCC 
AGACCAGGAC ACAGCCTAAT AGCTGCTATT CGGAGCCAAC ATCAACACGA GTCCTTGCCA 
CTGGCATACA ATGACAAGAT TGTGGCATTT CTTCGACAGC CAAACATTTT TGAAATGCTA 
CAAGAACGTC AGCCAAGCTT GGCAAGAAAC CACACACTCA GGGAGAAAAT CCATTATATT 
CGGACAGAGG GCAATCATGG ACTTGAGAAA TTGTCCTGTG ATGCAGATCT AGTCATTTTG 
CTAAGTCTCT TTGAAGAAGA GATCATGTCC TATGTCCCCC TGCAATCTGC CTTTCACCCT 
GGATATAGCT TCTCTCCCCG CTGTTCACCC TGTTCCTCAC CCCAGAACTC CCCAGGTTTA 
CAGAGAGCCA GTGCCAGGGC CCCTTCACCG TACCGAAGGG ATTTTGAAGC TAAGCTCCGC 
AATTTCTACC GAAAACTGGA AGCCAAAGGA TTTGGGCAGG GTCCGGGTAA AATTAAGCTC 
ATCATCCGTC GAGACCACTT GTTGGAGGGG ACCTTCAATC AGGTGATGGC ATACTCTCGG 
AAGGAGCTCC AGAGAAACAA GCTCTACATC ACCTTTGTTG GAGAGGAGGG GCTGGACTAC 
AGTGGCCCTT CTCGAGAGTT CTTCTTCCTG TTGTCTCAGG AGCTCTTCAA CCCTTACTAT 
GGACTCTTTG AGTATTCTGC AAATGACACT TACACAGTGC AGATCAGCCC CATGTCGGCG 
TTTGTAGAAA ATTATCTCGA ATGGTTTAGA TTCAGCGGCC GTATCCTGGG CCTAGCTCTG 
ATCCACCAGT ACCTCTTGGA TGCTTTCTTC ACCAGACCCT TCTATAAGGG ACTCCTGAAG 
CTGCCATGCG ATCTGAGTGA CCTGGAATAC TTGGATGAGG AATTCCACCA GAGTTTACAG 
TGGATGAAGG ACAACAACAT CACAGATATT CTTGACCTCA CTTTCACTGT TAACGAAGAA 
GTTTTTGGAC AGGTAACAGA AAGAGAGTTG AAGTCTGGAG GTGCCAACAC GCAGGTGACT 
GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAGT GGCGTGTAGA GCGTGGTGTG 
GTACAGCAGA CCGAGGCATT GGTCAGGGGT TTCTATGAGG TTGTAGACTC AAGGCTTGTC 
TCAGTGTTTG ACGCGAGGGA GCTGGAGCTG GTAATCGCGG GAACTGCTGA AATCGACCTG 
AATGACTGGA GGAATAACAC TGAGTACCGG GGAGGTTATC ATGATGGCCA CCTTGTGATC 
CGCTGGTTCT GGGCTGCAGT GGAGCGCTTC AATAATGAGC AGAGATTGAG ATTACTTCAG 
TTTGTGACAG GAACGTCGAG TGTTCCCTAT GAAGGCTTTG CAGCTCTGCG GGGGAGCAAT 
GGGCTTCGAA GGTTCTGCAT AGAGAAGTGG GGGAAGATTA CATCTCTTCC CAGGGCACAT 
ACATGCTTCA ACCGACTGGA TCTCCCACCG TATCCTTCAT ACTCCACATT GTATGAAAAG 
TTGTTAACAG CAGTTGAAGA AACCAGCACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099587.1
CDS	153..4937
Translation	MNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDGITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGYSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETVQSQGNRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGHLQFRFEITSSIHADDEEISLNAEPESAEVQDSVMNSVVENSSGEPPGDAAEFLKDLKPEAPSEEDGVRGSENENREHARPVEEAAGAMETRDDSKFSEGPEEVGELQDPEQHDTQPTLSAEEVAERLPLGEGCPPSLLPEESTALGSKAEEEAIPENGAGEEEMQEKGKDEEEEEGVSALEQGEPGLELRASVRKKSRPCSLPVSELETVIASACGEAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDAMEEESTLKESSEKDGLSEVDTIAADPQSMEDGENNGATLCMASSDCSGGHFSNLSKSIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARTTEGLDSPMAGPSNRREGDCPMLHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTIATERSEEDSGNQSCEQIPDGGAGGGGSDSEAESSQSNLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSTLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	NM_001106117.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106117.1

ORF Insert Sequence:

ATGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCTGCCA TGGCATCTCC ATCTAGAAAC 
TCCCAGAGCC GACGCCGGTG CAAAGAGCCC CTCCGGTACA GCTATAACCC AGACCAATTC 
CACAACATAG ACATCAGAAA TGGTGCCCAT GATGGCATCA CCATCCCTCG CTCTACCAGT 
GACACTGACC TGGTCACCTC AGACAGCCGC TCTACACTCA TGGTCAGCAG TTCCTACTAT 
TCTATAGGGT ATTCCCAGGA TTTGGTGATC CACTGGGACA TCAAGGAAGA AGTGGATGCC 
GGAGACTGGA TTGGGATGTA CCTTATTGGT GAAGTCTCAT CTGAAAACTT TCTGGACTAT 
AAGAACCGTG GAGTCAATGG ATCTCACAGA GGCCAGATCA TTTGGAAGAT TGATGCCAGC 
TCTTACTTTG TGGAATCTGA AACTAAGATC TGCTTCAAAT ACTACCACGG AGTAAGCGGG 
GCCCTGAGAG CTACAACCCC CAGTGTCACT GTCAAGAACT CAGCGGCTCC TATTTTTAAA 
GGTATTGGCT CTGAGGAGAC TGTGCAAAGT CAAGGAAACA GGAGGCTCAT CAGCTTCTCT 
CTCTCAGATT TCCAGGCCAT GGGATTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAT 
CTGAAGATTT CCATTCAACC TGGTAAACAT AGCATCTTCC CGGCCCTCCC TCATCATGGA 
CAGGAACGGA GATCCACGAT CATAGGCAAC ACTGTAAACC CCATCTGGCA GGCAGAGCAT 
TTCAGTTTCG TGTCTCTGCC AACTGATGTG CTGGAAATTG AAGTGAAGGA CAAGTTTGCC 
AAGAGTCGTC CCATCATCAA GCGTTTCTTG GGAAAGCTGT CAATGCCAGT TCAGAGGCTA 
CTGGAGAGAC ATGCCATAGG GGATAGGGTG GTCAGCTACA CACTTGGCCG CAGGCTTCCA 
ACTGACCATG TGAGTGGACA TCTGCAATTC CGATTTGAGA TCACATCCTC CATCCACGCA 
GACGATGAAG AGATCTCTCT GAATGCTGAG CCTGAGTCAG CGGAAGTGCA GGACAGTGTC 
ATGAATAGTG TGGTGGAAAA CAGCAGTGGG GAGCCTCCCG GTGATGCTGC AGAATTCCTC 
AAGGACTTGA AGCCAGAGGC ACCGAGTGAA GAGGATGGAG TGCGCGGCTC AGAAAATGAA 
AACCGAGAGC ATGCCAGGCC AGTCGAGGAA GCTGCGGGTG CTATGGAGAC CAGAGATGAT 
AGCAAGTTCT CTGAAGGACC GGAAGAGGTT GGAGAGCTTC AGGACCCAGA GCAACATGAC 
ACCCAGCCCA CTCTGAGCGC AGAAGAAGTG GCAGAGCGAC TTCCCCTGGG TGAGGGTTGC 
CCACCCTCGC TGTTGCCAGA AGAGAGTACA GCTCTAGGCA GCAAGGCAGA AGAGGAAGCA 
ATTCCAGAAA ATGGAGCGGG AGAGGAAGAG ATGCAGGAGA AGGGGAAAGA TGAGGAGGAA 
GAAGAGGGTG TTTCTGCCCT GGAGCAAGGA GAGCCTGGAC TTGAGCTGAG GGCCTCCGTG 
AGAAAAAAAA GCAGGCCATG TTCCTTACCT GTATCCGAGC TTGAAACAGT GATCGCATCT 
GCCTGTGGAG AGGCTGAGAC CCCAAGGACC CACTACATCC GCATCCACAC CCTACTGCAC 
AGCATGCCCT CGGCCCAGAG GGGAAGCACC ACAGAGGAGG AGGATGCCAT GGAGGAGGAA 
TCCACCCTCA AGGAGTCCTC TGAAAAGGAT GGGCTCAGCG AGGTGGACAC AATAGCTGCT 
GACCCACAGT CCATGGAAGA TGGCGAGAAC AATGGCGCTA CTCTCTGCAT GGCATCCTCT 
GACTGCTCTG GGGGCCATTT CTCCAATTTA TCTAAAAGCA TTGGCGCTGG CCAAGATGGC 
GAAGCTCACC CCAGCACTGG GAGTGAGAGT GACTCCAGCC CCCAGCAGGG AGCAGACCAC 
AGTTGCGAGG GCTGTGATGC CTCATGCTGT AGCCCCTCAT GCTATAGCAC ATCGTGCTAC 
AGCAGCTCCT GTTATAGCAG CTCCTGTTAC AGCAGCTCCT GCTACAATGG CAACAATCGG 
TTTGCCAGCC ACACACGCTT CTCCTCGGTG GACAGCGCCA AGATCTCTGA GAGCACAGTC 
TTTTCTTCAC AAGAGGATGA GGAGGAGGAA AACAGTGCAT TCGAGTCGGT ACCTGACTCG 
GTGCAAAGCC CAGAGCTAGA TCCCGAGTCT ACAAATGGGG CTGGGCCTTG GCAGGATGAA 
CTGGCTGCCC CTGGTGGGAA TGCAGCAAGA ACTACTGAAG GTCTGGACTC CCCCATGGCA 
GGCCCTAGCA ATAGGAGAGA AGGTGACTGT CCTATGCTCC ATAATTCCCA GCCAATAAGC 
CAGCTTCCTT CCTTGAGGCC TGAACATCAT CACTACCCAA CAATCGATGA GCCTCTTCCA 
CCAAACTGGG AAGCTCGAAT CGACAGCCAT GGACGGGTCT TTTATGTGGA CCATGTAAAT 
CGCACAACCA CCTGGCAGCG ACCATCAATG GCGCCTACCC CAGATGGAAT GATCAGATCC 
GGGTCTGTCC ACCAGATGGA GCAGCTTAAC AGAAGGTATC AAAACATCCA GAGAACCATT 
GCAACAGAGA GATCTGAAGA AGATTCTGGC AACCAAAGCT GTGAGCAAAT ACCAGACGGA 
GGAGCTGGAG GTGGAGGGAG TGACTCGGAA GCAGAATCTT CCCAATCAAA CTTAGATCTG 
AGGAGAGAAG GGTCACTTTC TCCTGTCAAC TCACAGAAGG TCACCTTGCT GCTGCAGTCT 
CCAGCAGTCA AGTTCATCAC CAATCCTGAG TTCTTCACTG TGTTGCATGC CAATTATAGT 
GCCTACAGGG TTTTTACAAG TAGCACTTGC CTGAAGCATA TGATTTTGAA AGTCCGAAGA 
GATGCTCGCA ATTTTGAACG CTACCAGCAC AACCGGGACC TGGTGAATTT CATCAACATG 
TTTGCAGATA CTCGGCTGGA ACTGCCCCGG GGTTGGGAGA TCAAAACTGA TCATCAGGGA 
AAGTCTTTCT TTGTGGACCA CAACAGTCGG GCTACTACTT TCATTGATCC CCGAATCCCT 
CTTCAGAATG GCCGCCTTCC CAATCATCTA ACCCACCGAC AGCACCTCCA GAGGCTCAGG 
AGTTACAGTG CTGGTGAGGC CTCAGAAGTC TCTAGAAACA GAGGAGCATC TTTGCTGGCC 
AGACCAGGAC ACAGCCTAAT AGCTGCTATT CGGAGCCAAC ATCAACACGA GTCCTTGCCA 
CTGGCATACA ATGACAAGAT TGTGGCATTT CTTCGACAGC CAAACATTTT TGAAATGCTA 
CAAGAACGTC AGCCAAGCTT GGCAAGAAAC CACACACTCA GGGAGAAAAT CCATTATATT 
CGGACAGAGG GCAATCATGG ACTTGAGAAA TTGTCCTGTG ATGCAGATCT AGTCATTTTG 
CTAAGTCTCT TTGAAGAAGA GATCATGTCC TATGTCCCCC TGCAATCTGC CTTTCACCCT 
GGATATAGCT TCTCTCCCCG CTGTTCACCC TGTTCCTCAC CCCAGAACTC CCCAGGTTTA 
CAGAGAGCCA GTGCCAGGGC CCCTTCACCG TACCGAAGGG ATTTTGAAGC TAAGCTCCGC 
AATTTCTACC GAAAACTGGA AGCCAAAGGA TTTGGGCAGG GTCCGGGTAA AATTAAGCTC 
ATCATCCGTC GAGACCACTT GTTGGAGGGG ACCTTCAATC AGGTGATGGC ATACTCTCGG 
AAGGAGCTCC AGAGAAACAA GCTCTACATC ACCTTTGTTG GAGAGGAGGG GCTGGACTAC 
AGTGGCCCTT CTCGAGAGTT CTTCTTCCTG TTGTCTCAGG AGCTCTTCAA CCCTTACTAT 
GGACTCTTTG AGTATTCTGC AAATGACACT TACACAGTGC AGATCAGCCC CATGTCGGCG 
TTTGTAGAAA ATTATCTCGA ATGGTTTAGA TTCAGCGGCC GTATCCTGGG CCTAGCTCTG 
ATCCACCAGT ACCTCTTGGA TGCTTTCTTC ACCAGACCCT TCTATAAGGG ACTCCTGAAG 
CTGCCATGCG ATCTGAGTGA CCTGGAATAC TTGGATGAGG AATTCCACCA GAGTTTACAG 
TGGATGAAGG ACAACAACAT CACAGATATT CTTGACCTCA CTTTCACTGT TAACGAAGAA 
GTTTTTGGAC AGGTAACAGA AAGAGAGTTG AAGTCTGGAG GTGCCAACAC GCAGGTGACT 
GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAGT GGCGTGTAGA GCGTGGTGTG 
GTACAGCAGA CCGAGGCATT GGTCAGGGGT TTCTATGAGG TTGTAGACTC AAGGCTTGTC 
TCAGTGTTTG ACGCGAGGGA GCTGGAGCTG GTAATCGCGG GAACTGCTGA AATCGACCTG 
AATGACTGGA GGAATAACAC TGAGTACCGG GGAGGTTATC ATGATGGCCA CCTTGTGATC 
CGCTGGTTCT GGGCTGCAGT GGAGCGCTTC AATAATGAGC AGAGATTGAG ATTACTTCAG 
TTTGTGACAG GAACGTCGAG TGTTCCCTAT GAAGGCTTTG CAGCTCTGCG GGGGAGCAAT 
GGGCTTCGAA GGTTCTGCAT AGAGAAGTGG GGGAAGATTA CATCTCTTCC CAGGGCACAT 
ACATGCTTCA ACCGACTGGA TCTCCCACCG TATCCTTCAT ACTCCACATT GTATGAAAAG 
TTGTTAACAG CAGTTGAAGA AACCAGCACC TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_008771697.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008769919.1 Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X2, mRNA.

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CloneID	ORa44901
Clone ID Related Accession (Same CDS sequence)	XM_008771697.2 , XM_017600486.1
Accession Version	XM_008771697.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008771697.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCAT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA GCCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGGC 
ATCACCATCC CTCGCTCTAC CAGTGACACT GACCTGGTCA CCTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCTATA GGGTATTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCCGGAGAC TGGATTGGGA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGATCTCA CAGAGGCCAG 
ATCATTTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG CGGGGCCCTG AGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCGG CTCCTATTTT TAAAGGTATT GGCTCTGAGG AGACTGTGCA AAGTCAAGGA 
AACAGGAGGC TCATCAGCTT CTCTCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AACCTGGTAA ACATAGCATC 
TTCCCGGCCC TCCCTCATCA TGGACAGGAA CGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACTGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CAGTTCAGAG GCTACTGGAG AGACATGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACTGAC CATGTGAGTG GACATCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA CGCAGACGAT GAAGAGATCT CTCTGAATGC TGAGCCTGAG 
TCAGCGGAAG TGCAGGACAG TGTCATGAAT AGTGTGGTGG AAAACAGCAG TGGGGAGCCT 
CCCGGTGATG CTGCAGAATT CCTCAAGGAC TTGAAGCCAG AGGCACCGAG TGAAGAGGAT 
GGAGTGCGCG GCTCAGAAAA TGAAAACCGA GAGCATGCCA GGCCAGTCGA GGAAGCTGCG 
GGTGCTATGG AGACCAGAGA TGATAGCAAG TTCTCTGAAG GACCGGAAGA GGTTGGAGAG 
CTTCAGGACC CAGAGCAACA TGACACCCAG CCCACTCTGA GCGCAGAAGA AGTGGCAGAG 
CGACTTCCCC TGGGTGAGGG TTGCCCACCC TCGCTGTTGC CAGAAGAGAG TACAGCTCTA 
GGCAGCAAGG CAGAAGAGGA AGCAATTCCA GAAAATGGAG CGGGAGAGGA AGAGATGCAG 
GAGAAGGGGA AAGATGAGGA GGAAGAAGAG GGTGTTTCTG CCCTGGAGCA AGGAGAGCCT 
GGACTTGAGC TGAGGGCCTC CGTGAGAAAA AAAAGCAGGC CATGTTCCTT ACCTGTATCC 
GAGCTTGAAA CAGTGATCGC ATCTGCCTGT GGAGAGGCTG AGACCCCAAG GACCCACTAC 
ATCCGCATCC ACACCCTACT GCACAGCATG CCCTCGGCCC AGAGGGGAAG CACCACAGAG 
GAGGAGGATG CCATGGAGGA GGAATCCACC CTCAAGGAGT CCTCTGAAAA GGATGGGCTC 
AGCGAGGTGG ACACAATAGC TGCTGACCCA CAGTCCATGG AAGATGGCGA GAACAATGGC 
GCTACTCTCT GCATGGCATC CTCTGACTGC TCTGGGGGCC ATTTCTCCAA TTTATCTAAA 
AGCATTGGCG CTGGCCAAGA TGGCGAAGCT CACCCCAGCA CTGGGAGTGA GAGTGACTCC 
AGCCCCCAGC AGGGAGCAGA CCACAGTTGC GAGGGCTGTG ATGCCTCATG CTGTAGCCCC 
TCATGCTATA GCACATCGTG CTACAGCAGC TCCTGTTATA GCAGCTCCTG TTACAGCAGC 
TCCTGCTACA ATGGCAACAA TCGGTTTGCC AGCCACACAC GCTTCTCCTC GGTGGACAGC 
GCCAAGATCT CTGAGAGCAC AGTCTTTTCT TCACAAGAGG ATGAGGAGGA GGAAAACAGT 
GCATTCGAGT CGGTACCTGA CTCGGTGCAA AGCCCAGAGC TAGATCCCGA GTCTACAAAT 
GGGGCTGGGC CTTGGCAGGA TGAACTGGCT GCCCCTGGTG GGAATGCAGC AAGAACTACT 
GAAGGTCTGG ACTCCCCCAT GGCAGGCCCT AGCAATAGGA GAGAAGGTGA CTGTCCTATG 
CTCCATAATT CCCAGCCAAT AAGCCAGCTT CCTTCCTTGA GGCCTGAACA TCATCACTAC 
CCAACAATCG ATGAGCCTCT TCCACCAAAC TGGGAAGCTC GAATCGACAG CCATGGACGG 
GTCTTTTATG TGGACCATGT AAATCGCACA ACCACCTGGC AGCGACCATC AATGGCGCCT 
ACCCCAGATG GAATGATCAG ATCCGGGTCT GTCCACCAGA TGGAGCAGCT TAACAGAAGG 
TATCAAAACA TCCAGAGAAC CATTGCAACA GAGAGATCTG AAGAAGATTC TGGCAACCAA 
AGCTGTGAGC AAATACCAGA CGGAGGAGCT GGAGGTGGAG GGAGTGACTC GGAAGCAGAA 
TCTTCCCAAT CAAACTTAGA TCTGAGGAGA GAAGGGTCAC TTTCTCCTGT CAACTCACAG 
AAGGTCACCT TGCTGCTGCA GTCTCCAGCA GTCAAGTTCA TCACCAATCC TGAGTTCTTC 
ACTGTGTTGC ATGCCAATTA TAGTGCCTAC AGGGTTTTTA CAAGTAGCAC TTGCCTGAAG 
CATATGATTT TGAAAGTCCG AAGAGATGCT CGCAATTTTG AACGCTACCA GCACAACCGG 
GACCTGGTGA ATTTCATCAA CATGTTTGCA GATACTCGGC TGGAACTGCC CCGGGGTTGG 
GAGATCAAAA CTGATCATCA GGGAAAGTCT TTCTTTGTGG ACCACAACAG TCGGGCTACT 
ACTTTCATTG ATCCCCGAAT CCCTCTTCAG AATGGCCGCC TTCCCAATCA TCTAACCCAC 
CGACAGCACC TCCAGAGGCT CAGGAGTTAC AGTGCTGGTG AGGCCTCAGA AGTCTCTAGA 
AACAGAGGAG CATCTTTGCT GGCCAGACCA GGACACAGCC TAATAGCTGC TATTCGGAGC 
CAACATCAAC ACGAGTCCTT GCCACTGGCA TACAATGACA AGATTGTGGC ATTTCTTCGA 
CAGCCAAACA TTTTTGAAAT GCTACAAGAA CGTCAGCCAA GCTTGGCAAG AAACCACACA 
CTCAGGGAGA AAATCCATTA TATTCGGACA GAGGGCAATC ATGGACTTGA GAAATTGTCC 
TGTGATGCAG ATCTAGTCAT TTTGCTAAGT CTCTTTGAAG AAGAGATCAT GTCCTATGTC 
CCCCTGCAAT CTGCCTTTCA CCCTGGATAT AGCTTCTCTC CCCGCTGTTC ACCCTGTTCC 
TCACCCCAGA ACTCCCCAGG TTTACAGAGA GCCAGTGCCA GGGCCCCTTC ACCGTACCGA 
AGGGATTTTG AAGCTAAGCT CCGCAATTTC TACCGAAAAC TGGAAGCCAA AGGATTTGGG 
CAGGGTCCGG GTAAAATTAA GCTCATCATC CGTCGAGACC ACTTGTTGGA GGGGACCTTC 
AATCAGGTGA TGGCATACTC TCGGAAGGAG CTCCAGAGAA ACAAGCTCTA CATCACCTTT 
GTTGGAGAGG AGGGGCTGGA CTACAGTGGC CCTTCTCGAG AGTTCTTCTT CCTGTTGTCT 
CAGGAGCTCT TCAACCCTTA CTATGGACTC TTTGAGTATT CTGCAAATGA CACTTACACA 
GTGCAGATCA GCCCCATGTC GGCGTTTGTA GAAAATTATC TCGAATGGTT TAGATTCAGC 
GGCCGTATCC TGGGCCTAGC TCTGATCCAC CAGTACCTCT TGGATGCTTT CTTCACCAGA 
CCCTTCTATA AGGGACTCCT GAAGCTGCCA TGCGATCTGA GTGACCTGGA ATACTTGGAT 
GAGGAATTCC ACCAGAGTTT ACAGTGGATG AAGGACAACA ACATCACAGA TATTCTTGAC 
CTCACTTTCA CTGTTAACGA AGAAGTTTTT GGACAGGTAA CAGAAAGAGA GTTGAAGTCT 
GGAGGTGCCA ACACGCAGGT GACTGAGAAG AACAAGAAGG AGTACATCGA GAGGATGGTG 
AAGTGGCGTG TAGAGCGTGG TGTGGTACAG CAGACCGAGG CATTGGTCAG GGGTTTCTAT 
GAGGTTGTAG ACTCAAGGCT TGTCTCAGTG TTTGACGCGA GGGAGCTGGA GCTGGTAATC 
GCGGGAACTG CTGAAATCGA CCTGAATGAC TGGAGGAATA ACACTGAGTA CCGGGGAGGT 
TATCATGATG GCCACCTTGT GATCCGCTGG TTCTGGGCTG CAGTGGAGCG CTTCAATAAT 
GAGCAGAGAT TGAGATTACT TCAGTTTGTG ACAGGAACGT CGAGTGTTCC CTATGAAGGC 
TTTGCAGCTC TGCGGGGGAG CAATGGGCTT CGAAGGTTCT GCATAGAGAA GTGGGGGAAG 
ATTACATCTC TTCCCAGGGC ACATACATGC TTCAACCGAC TGGATCTCCC ACCGTATCCT 
TCATACTCCA CATTGTATGA AAAGTTGTTA ACAGCAGTTG AAGAAACCAG CACCTTTGGA 
CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008769919.1
CDS	106..4914
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDGITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGYSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETVQSQGNRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGHLQFRFEITSSIHADDEEISLNAEPESAEVQDSVMNSVVENSSGEPPGDAAEFLKDLKPEAPSEEDGVRGSENENREHARPVEEAAGAMETRDDSKFSEGPEEVGELQDPEQHDTQPTLSAEEVAERLPLGEGCPPSLLPEESTALGSKAEEEAIPENGAGEEEMQEKGKDEEEEEGVSALEQGEPGLELRASVRKKSRPCSLPVSELETVIASACGEAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDAMEEESTLKESSEKDGLSEVDTIAADPQSMEDGENNGATLCMASSDCSGGHFSNLSKSIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARTTEGLDSPMAGPSNRREGDCPMLHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTIATERSEEDSGNQSCEQIPDGGAGGGGSDSEAESSQSNLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSTLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_008771697.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008771697.2

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCAT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA GCCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGGC 
ATCACCATCC CTCGCTCTAC CAGTGACACT GACCTGGTCA CCTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCTATA GGGTATTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCCGGAGAC TGGATTGGGA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGATCTCA CAGAGGCCAG 
ATCATTTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG CGGGGCCCTG AGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCGG CTCCTATTTT TAAAGGTATT GGCTCTGAGG AGACTGTGCA AAGTCAAGGA 
AACAGGAGGC TCATCAGCTT CTCTCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AACCTGGTAA ACATAGCATC 
TTCCCGGCCC TCCCTCATCA TGGACAGGAA CGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACTGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CAGTTCAGAG GCTACTGGAG AGACATGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACTGAC CATGTGAGTG GACATCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA CGCAGACGAT GAAGAGATCT CTCTGAATGC TGAGCCTGAG 
TCAGCGGAAG TGCAGGACAG TGTCATGAAT AGTGTGGTGG AAAACAGCAG TGGGGAGCCT 
CCCGGTGATG CTGCAGAATT CCTCAAGGAC TTGAAGCCAG AGGCACCGAG TGAAGAGGAT 
GGAGTGCGCG GCTCAGAAAA TGAAAACCGA GAGCATGCCA GGCCAGTCGA GGAAGCTGCG 
GGTGCTATGG AGACCAGAGA TGATAGCAAG TTCTCTGAAG GACCGGAAGA GGTTGGAGAG 
CTTCAGGACC CAGAGCAACA TGACACCCAG CCCACTCTGA GCGCAGAAGA AGTGGCAGAG 
CGACTTCCCC TGGGTGAGGG TTGCCCACCC TCGCTGTTGC CAGAAGAGAG TACAGCTCTA 
GGCAGCAAGG CAGAAGAGGA AGCAATTCCA GAAAATGGAG CGGGAGAGGA AGAGATGCAG 
GAGAAGGGGA AAGATGAGGA GGAAGAAGAG GGTGTTTCTG CCCTGGAGCA AGGAGAGCCT 
GGACTTGAGC TGAGGGCCTC CGTGAGAAAA AAAAGCAGGC CATGTTCCTT ACCTGTATCC 
GAGCTTGAAA CAGTGATCGC ATCTGCCTGT GGAGAGGCTG AGACCCCAAG GACCCACTAC 
ATCCGCATCC ACACCCTACT GCACAGCATG CCCTCGGCCC AGAGGGGAAG CACCACAGAG 
GAGGAGGATG CCATGGAGGA GGAATCCACC CTCAAGGAGT CCTCTGAAAA GGATGGGCTC 
AGCGAGGTGG ACACAATAGC TGCTGACCCA CAGTCCATGG AAGATGGCGA GAACAATGGC 
GCTACTCTCT GCATGGCATC CTCTGACTGC TCTGGGGGCC ATTTCTCCAA TTTATCTAAA 
AGCATTGGCG CTGGCCAAGA TGGCGAAGCT CACCCCAGCA CTGGGAGTGA GAGTGACTCC 
AGCCCCCAGC AGGGAGCAGA CCACAGTTGC GAGGGCTGTG ATGCCTCATG CTGTAGCCCC 
TCATGCTATA GCACATCGTG CTACAGCAGC TCCTGTTATA GCAGCTCCTG TTACAGCAGC 
TCCTGCTACA ATGGCAACAA TCGGTTTGCC AGCCACACAC GCTTCTCCTC GGTGGACAGC 
GCCAAGATCT CTGAGAGCAC AGTCTTTTCT TCACAAGAGG ATGAGGAGGA GGAAAACAGT 
GCATTCGAGT CGGTACCTGA CTCGGTGCAA AGCCCAGAGC TAGATCCCGA GTCTACAAAT 
GGGGCTGGGC CTTGGCAGGA TGAACTGGCT GCCCCTGGTG GGAATGCAGC AAGAACTACT 
GAAGGTCTGG ACTCCCCCAT GGCAGGCCCT AGCAATAGGA GAGAAGGTGA CTGTCCTATG 
CTCCATAATT CCCAGCCAAT AAGCCAGCTT CCTTCCTTGA GGCCTGAACA TCATCACTAC 
CCAACAATCG ATGAGCCTCT TCCACCAAAC TGGGAAGCTC GAATCGACAG CCATGGACGG 
GTCTTTTATG TGGACCATGT AAATCGCACA ACCACCTGGC AGCGACCATC AATGGCGCCT 
ACCCCAGATG GAATGATCAG ATCCGGGTCT GTCCACCAGA TGGAGCAGCT TAACAGAAGG 
TATCAAAACA TCCAGAGAAC CATTGCAACA GAGAGATCTG AAGAAGATTC TGGCAACCAA 
AGCTGTGAGC AAATACCAGA CGGAGGAGCT GGAGGTGGAG GGAGTGACTC GGAAGCAGAA 
TCTTCCCAAT CAAACTTAGA TCTGAGGAGA GAAGGGTCAC TTTCTCCTGT CAACTCACAG 
AAGGTCACCT TGCTGCTGCA GTCTCCAGCA GTCAAGTTCA TCACCAATCC TGAGTTCTTC 
ACTGTGTTGC ATGCCAATTA TAGTGCCTAC AGGGTTTTTA CAAGTAGCAC TTGCCTGAAG 
CATATGATTT TGAAAGTCCG AAGAGATGCT CGCAATTTTG AACGCTACCA GCACAACCGG 
GACCTGGTGA ATTTCATCAA CATGTTTGCA GATACTCGGC TGGAACTGCC CCGGGGTTGG 
GAGATCAAAA CTGATCATCA GGGAAAGTCT TTCTTTGTGG ACCACAACAG TCGGGCTACT 
ACTTTCATTG ATCCCCGAAT CCCTCTTCAG AATGGCCGCC TTCCCAATCA TCTAACCCAC 
CGACAGCACC TCCAGAGGCT CAGGAGTTAC AGTGCTGGTG AGGCCTCAGA AGTCTCTAGA 
AACAGAGGAG CATCTTTGCT GGCCAGACCA GGACACAGCC TAATAGCTGC TATTCGGAGC 
CAACATCAAC ACGAGTCCTT GCCACTGGCA TACAATGACA AGATTGTGGC ATTTCTTCGA 
CAGCCAAACA TTTTTGAAAT GCTACAAGAA CGTCAGCCAA GCTTGGCAAG AAACCACACA 
CTCAGGGAGA AAATCCATTA TATTCGGACA GAGGGCAATC ATGGACTTGA GAAATTGTCC 
TGTGATGCAG ATCTAGTCAT TTTGCTAAGT CTCTTTGAAG AAGAGATCAT GTCCTATGTC 
CCCCTGCAAT CTGCCTTTCA CCCTGGATAT AGCTTCTCTC CCCGCTGTTC ACCCTGTTCC 
TCACCCCAGA ACTCCCCAGG TTTACAGAGA GCCAGTGCCA GGGCCCCTTC ACCGTACCGA 
AGGGATTTTG AAGCTAAGCT CCGCAATTTC TACCGAAAAC TGGAAGCCAA AGGATTTGGG 
CAGGGTCCGG GTAAAATTAA GCTCATCATC CGTCGAGACC ACTTGTTGGA GGGGACCTTC 
AATCAGGTGA TGGCATACTC TCGGAAGGAG CTCCAGAGAA ACAAGCTCTA CATCACCTTT 
GTTGGAGAGG AGGGGCTGGA CTACAGTGGC CCTTCTCGAG AGTTCTTCTT CCTGTTGTCT 
CAGGAGCTCT TCAACCCTTA CTATGGACTC TTTGAGTATT CTGCAAATGA CACTTACACA 
GTGCAGATCA GCCCCATGTC GGCGTTTGTA GAAAATTATC TCGAATGGTT TAGATTCAGC 
GGCCGTATCC TGGGCCTAGC TCTGATCCAC CAGTACCTCT TGGATGCTTT CTTCACCAGA 
CCCTTCTATA AGGGACTCCT GAAGCTGCCA TGCGATCTGA GTGACCTGGA ATACTTGGAT 
GAGGAATTCC ACCAGAGTTT ACAGTGGATG AAGGACAACA ACATCACAGA TATTCTTGAC 
CTCACTTTCA CTGTTAACGA AGAAGTTTTT GGACAGGTAA CAGAAAGAGA GTTGAAGTCT 
GGAGGTGCCA ACACGCAGGT GACTGAGAAG AACAAGAAGG AGTACATCGA GAGGATGGTG 
AAGTGGCGTG TAGAGCGTGG TGTGGTACAG CAGACCGAGG CATTGGTCAG GGGTTTCTAT 
GAGGTTGTAG ACTCAAGGCT TGTCTCAGTG TTTGACGCGA GGGAGCTGGA GCTGGTAATC 
GCGGGAACTG CTGAAATCGA CCTGAATGAC TGGAGGAATA ACACTGAGTA CCGGGGAGGT 
TATCATGATG GCCACCTTGT GATCCGCTGG TTCTGGGCTG CAGTGGAGCG CTTCAATAAT 
GAGCAGAGAT TGAGATTACT TCAGTTTGTG ACAGGAACGT CGAGTGTTCC CTATGAAGGC 
TTTGCAGCTC TGCGGGGGAG CAATGGGCTT CGAAGGTTCT GCATAGAGAA GTGGGGGAAG 
ATTACATCTC TTCCCAGGGC ACATACATGC TTCAACCGAC TGGATCTCCC ACCGTATCCT 
TCATACTCCA CATTGTATGA AAAGTTGTTA ACAGCAGTTG AAGAAACCAG CACCTTTGGA 
CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_017600486.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455975.1 Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa44901
Clone ID Related Accession (Same CDS sequence)	XM_008771697.2 , XM_017600486.1
Accession Version	XM_017600486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCAT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA GCCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGGC 
ATCACCATCC CTCGCTCTAC CAGTGACACT GACCTGGTCA CCTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCTATA GGGTATTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCCGGAGAC TGGATTGGGA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGATCTCA CAGAGGCCAG 
ATCATTTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG CGGGGCCCTG AGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCGG CTCCTATTTT TAAAGGTATT GGCTCTGAGG AGACTGTGCA AAGTCAAGGA 
AACAGGAGGC TCATCAGCTT CTCTCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AACCTGGTAA ACATAGCATC 
TTCCCGGCCC TCCCTCATCA TGGACAGGAA CGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACTGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CAGTTCAGAG GCTACTGGAG AGACATGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACTGAC CATGTGAGTG GACATCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA CGCAGACGAT GAAGAGATCT CTCTGAATGC TGAGCCTGAG 
TCAGCGGAAG TGCAGGACAG TGTCATGAAT AGTGTGGTGG AAAACAGCAG TGGGGAGCCT 
CCCGGTGATG CTGCAGAATT CCTCAAGGAC TTGAAGCCAG AGGCACCGAG TGAAGAGGAT 
GGAGTGCGCG GCTCAGAAAA TGAAAACCGA GAGCATGCCA GGCCAGTCGA GGAAGCTGCG 
GGTGCTATGG AGACCAGAGA TGATAGCAAG TTCTCTGAAG GACCGGAAGA GGTTGGAGAG 
CTTCAGGACC CAGAGCAACA TGACACCCAG CCCACTCTGA GCGCAGAAGA AGTGGCAGAG 
CGACTTCCCC TGGGTGAGGG TTGCCCACCC TCGCTGTTGC CAGAAGAGAG TACAGCTCTA 
GGCAGCAAGG CAGAAGAGGA AGCAATTCCA GAAAATGGAG CGGGAGAGGA AGAGATGCAG 
GAGAAGGGGA AAGATGAGGA GGAAGAAGAG GGTGTTTCTG CCCTGGAGCA AGGAGAGCCT 
GGACTTGAGC TGAGGGCCTC CGTGAGAAAA AAAAGCAGGC CATGTTCCTT ACCTGTATCC 
GAGCTTGAAA CAGTGATCGC ATCTGCCTGT GGAGAGGCTG AGACCCCAAG GACCCACTAC 
ATCCGCATCC ACACCCTACT GCACAGCATG CCCTCGGCCC AGAGGGGAAG CACCACAGAG 
GAGGAGGATG CCATGGAGGA GGAATCCACC CTCAAGGAGT CCTCTGAAAA GGATGGGCTC 
AGCGAGGTGG ACACAATAGC TGCTGACCCA CAGTCCATGG AAGATGGCGA GAACAATGGC 
GCTACTCTCT GCATGGCATC CTCTGACTGC TCTGGGGGCC ATTTCTCCAA TTTATCTAAA 
AGCATTGGCG CTGGCCAAGA TGGCGAAGCT CACCCCAGCA CTGGGAGTGA GAGTGACTCC 
AGCCCCCAGC AGGGAGCAGA CCACAGTTGC GAGGGCTGTG ATGCCTCATG CTGTAGCCCC 
TCATGCTATA GCACATCGTG CTACAGCAGC TCCTGTTATA GCAGCTCCTG TTACAGCAGC 
TCCTGCTACA ATGGCAACAA TCGGTTTGCC AGCCACACAC GCTTCTCCTC GGTGGACAGC 
GCCAAGATCT CTGAGAGCAC AGTCTTTTCT TCACAAGAGG ATGAGGAGGA GGAAAACAGT 
GCATTCGAGT CGGTACCTGA CTCGGTGCAA AGCCCAGAGC TAGATCCCGA GTCTACAAAT 
GGGGCTGGGC CTTGGCAGGA TGAACTGGCT GCCCCTGGTG GGAATGCAGC AAGAACTACT 
GAAGGTCTGG ACTCCCCCAT GGCAGGCCCT AGCAATAGGA GAGAAGGTGA CTGTCCTATG 
CTCCATAATT CCCAGCCAAT AAGCCAGCTT CCTTCCTTGA GGCCTGAACA TCATCACTAC 
CCAACAATCG ATGAGCCTCT TCCACCAAAC TGGGAAGCTC GAATCGACAG CCATGGACGG 
GTCTTTTATG TGGACCATGT AAATCGCACA ACCACCTGGC AGCGACCATC AATGGCGCCT 
ACCCCAGATG GAATGATCAG ATCCGGGTCT GTCCACCAGA TGGAGCAGCT TAACAGAAGG 
TATCAAAACA TCCAGAGAAC CATTGCAACA GAGAGATCTG AAGAAGATTC TGGCAACCAA 
AGCTGTGAGC AAATACCAGA CGGAGGAGCT GGAGGTGGAG GGAGTGACTC GGAAGCAGAA 
TCTTCCCAAT CAAACTTAGA TCTGAGGAGA GAAGGGTCAC TTTCTCCTGT CAACTCACAG 
AAGGTCACCT TGCTGCTGCA GTCTCCAGCA GTCAAGTTCA TCACCAATCC TGAGTTCTTC 
ACTGTGTTGC ATGCCAATTA TAGTGCCTAC AGGGTTTTTA CAAGTAGCAC TTGCCTGAAG 
CATATGATTT TGAAAGTCCG AAGAGATGCT CGCAATTTTG AACGCTACCA GCACAACCGG 
GACCTGGTGA ATTTCATCAA CATGTTTGCA GATACTCGGC TGGAACTGCC CCGGGGTTGG 
GAGATCAAAA CTGATCATCA GGGAAAGTCT TTCTTTGTGG ACCACAACAG TCGGGCTACT 
ACTTTCATTG ATCCCCGAAT CCCTCTTCAG AATGGCCGCC TTCCCAATCA TCTAACCCAC 
CGACAGCACC TCCAGAGGCT CAGGAGTTAC AGTGCTGGTG AGGCCTCAGA AGTCTCTAGA 
AACAGAGGAG CATCTTTGCT GGCCAGACCA GGACACAGCC TAATAGCTGC TATTCGGAGC 
CAACATCAAC ACGAGTCCTT GCCACTGGCA TACAATGACA AGATTGTGGC ATTTCTTCGA 
CAGCCAAACA TTTTTGAAAT GCTACAAGAA CGTCAGCCAA GCTTGGCAAG AAACCACACA 
CTCAGGGAGA AAATCCATTA TATTCGGACA GAGGGCAATC ATGGACTTGA GAAATTGTCC 
TGTGATGCAG ATCTAGTCAT TTTGCTAAGT CTCTTTGAAG AAGAGATCAT GTCCTATGTC 
CCCCTGCAAT CTGCCTTTCA CCCTGGATAT AGCTTCTCTC CCCGCTGTTC ACCCTGTTCC 
TCACCCCAGA ACTCCCCAGG TTTACAGAGA GCCAGTGCCA GGGCCCCTTC ACCGTACCGA 
AGGGATTTTG AAGCTAAGCT CCGCAATTTC TACCGAAAAC TGGAAGCCAA AGGATTTGGG 
CAGGGTCCGG GTAAAATTAA GCTCATCATC CGTCGAGACC ACTTGTTGGA GGGGACCTTC 
AATCAGGTGA TGGCATACTC TCGGAAGGAG CTCCAGAGAA ACAAGCTCTA CATCACCTTT 
GTTGGAGAGG AGGGGCTGGA CTACAGTGGC CCTTCTCGAG AGTTCTTCTT CCTGTTGTCT 
CAGGAGCTCT TCAACCCTTA CTATGGACTC TTTGAGTATT CTGCAAATGA CACTTACACA 
GTGCAGATCA GCCCCATGTC GGCGTTTGTA GAAAATTATC TCGAATGGTT TAGATTCAGC 
GGCCGTATCC TGGGCCTAGC TCTGATCCAC CAGTACCTCT TGGATGCTTT CTTCACCAGA 
CCCTTCTATA AGGGACTCCT GAAGCTGCCA TGCGATCTGA GTGACCTGGA ATACTTGGAT 
GAGGAATTCC ACCAGAGTTT ACAGTGGATG AAGGACAACA ACATCACAGA TATTCTTGAC 
CTCACTTTCA CTGTTAACGA AGAAGTTTTT GGACAGGTAA CAGAAAGAGA GTTGAAGTCT 
GGAGGTGCCA ACACGCAGGT GACTGAGAAG AACAAGAAGG AGTACATCGA GAGGATGGTG 
AAGTGGCGTG TAGAGCGTGG TGTGGTACAG CAGACCGAGG CATTGGTCAG GGGTTTCTAT 
GAGGTTGTAG ACTCAAGGCT TGTCTCAGTG TTTGACGCGA GGGAGCTGGA GCTGGTAATC 
GCGGGAACTG CTGAAATCGA CCTGAATGAC TGGAGGAATA ACACTGAGTA CCGGGGAGGT 
TATCATGATG GCCACCTTGT GATCCGCTGG TTCTGGGCTG CAGTGGAGCG CTTCAATAAT 
GAGCAGAGAT TGAGATTACT TCAGTTTGTG ACAGGAACGT CGAGTGTTCC CTATGAAGGC 
TTTGCAGCTC TGCGGGGGAG CAATGGGCTT CGAAGGTTCT GCATAGAGAA GTGGGGGAAG 
ATTACATCTC TTCCCAGGGC ACATACATGC TTCAACCGAC TGGATCTCCC ACCGTATCCT 
TCATACTCCA CATTGTATGA AAAGTTGTTA ACAGCAGTTG AAGAAACCAG CACCTTTGGA 
CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455975.1
CDS	110..4918
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDGITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGYSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETVQSQGNRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGHLQFRFEITSSIHADDEEISLNAEPESAEVQDSVMNSVVENSSGEPPGDAAEFLKDLKPEAPSEEDGVRGSENENREHARPVEEAAGAMETRDDSKFSEGPEEVGELQDPEQHDTQPTLSAEEVAERLPLGEGCPPSLLPEESTALGSKAEEEAIPENGAGEEEMQEKGKDEEEEEGVSALEQGEPGLELRASVRKKSRPCSLPVSELETVIASACGEAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDAMEEESTLKESSEKDGLSEVDTIAADPQSMEDGENNGATLCMASSDCSGGHFSNLSKSIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARTTEGLDSPMAGPSNRREGDCPMLHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTIATERSEEDSGNQSCEQIPDGGAGGGGSDSEAESSQSNLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSTLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017600486.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600486.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCT 
GCCATGGCAT CTCCATCTAG AAACTCCCAG AGCCGACGCC GGTGCAAAGA GCCCCTCCGG 
TACAGCTATA ACCCAGACCA ATTCCACAAC ATAGACATCA GAAATGGTGC CCATGATGGC 
ATCACCATCC CTCGCTCTAC CAGTGACACT GACCTGGTCA CCTCAGACAG CCGCTCTACA 
CTCATGGTCA GCAGTTCCTA CTATTCTATA GGGTATTCCC AGGATTTGGT GATCCACTGG 
GACATCAAGG AAGAAGTGGA TGCCGGAGAC TGGATTGGGA TGTACCTTAT TGGTGAAGTC 
TCATCTGAAA ACTTTCTGGA CTATAAGAAC CGTGGAGTCA ATGGATCTCA CAGAGGCCAG 
ATCATTTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAT CTGAAACTAA GATCTGCTTC 
AAATACTACC ACGGAGTAAG CGGGGCCCTG AGAGCTACAA CCCCCAGTGT CACTGTCAAG 
AACTCAGCGG CTCCTATTTT TAAAGGTATT GGCTCTGAGG AGACTGTGCA AAGTCAAGGA 
AACAGGAGGC TCATCAGCTT CTCTCTCTCA GATTTCCAGG CCATGGGATT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AACCTGGTAA ACATAGCATC 
TTCCCGGCCC TCCCTCATCA TGGACAGGAA CGGAGATCCA CGATCATAGG CAACACTGTA 
AACCCCATCT GGCAGGCAGA GCATTTCAGT TTCGTGTCTC TGCCAACTGA TGTGCTGGAA 
ATTGAAGTGA AGGACAAGTT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT CTTGGGAAAG 
CTGTCAATGC CAGTTCAGAG GCTACTGGAG AGACATGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACTGAC CATGTGAGTG GACATCTGCA ATTCCGATTT 
GAGATCACAT CCTCCATCCA CGCAGACGAT GAAGAGATCT CTCTGAATGC TGAGCCTGAG 
TCAGCGGAAG TGCAGGACAG TGTCATGAAT AGTGTGGTGG AAAACAGCAG TGGGGAGCCT 
CCCGGTGATG CTGCAGAATT CCTCAAGGAC TTGAAGCCAG AGGCACCGAG TGAAGAGGAT 
GGAGTGCGCG GCTCAGAAAA TGAAAACCGA GAGCATGCCA GGCCAGTCGA GGAAGCTGCG 
GGTGCTATGG AGACCAGAGA TGATAGCAAG TTCTCTGAAG GACCGGAAGA GGTTGGAGAG 
CTTCAGGACC CAGAGCAACA TGACACCCAG CCCACTCTGA GCGCAGAAGA AGTGGCAGAG 
CGACTTCCCC TGGGTGAGGG TTGCCCACCC TCGCTGTTGC CAGAAGAGAG TACAGCTCTA 
GGCAGCAAGG CAGAAGAGGA AGCAATTCCA GAAAATGGAG CGGGAGAGGA AGAGATGCAG 
GAGAAGGGGA AAGATGAGGA GGAAGAAGAG GGTGTTTCTG CCCTGGAGCA AGGAGAGCCT 
GGACTTGAGC TGAGGGCCTC CGTGAGAAAA AAAAGCAGGC CATGTTCCTT ACCTGTATCC 
GAGCTTGAAA CAGTGATCGC ATCTGCCTGT GGAGAGGCTG AGACCCCAAG GACCCACTAC 
ATCCGCATCC ACACCCTACT GCACAGCATG CCCTCGGCCC AGAGGGGAAG CACCACAGAG 
GAGGAGGATG CCATGGAGGA GGAATCCACC CTCAAGGAGT CCTCTGAAAA GGATGGGCTC 
AGCGAGGTGG ACACAATAGC TGCTGACCCA CAGTCCATGG AAGATGGCGA GAACAATGGC 
GCTACTCTCT GCATGGCATC CTCTGACTGC TCTGGGGGCC ATTTCTCCAA TTTATCTAAA 
AGCATTGGCG CTGGCCAAGA TGGCGAAGCT CACCCCAGCA CTGGGAGTGA GAGTGACTCC 
AGCCCCCAGC AGGGAGCAGA CCACAGTTGC GAGGGCTGTG ATGCCTCATG CTGTAGCCCC 
TCATGCTATA GCACATCGTG CTACAGCAGC TCCTGTTATA GCAGCTCCTG TTACAGCAGC 
TCCTGCTACA ATGGCAACAA TCGGTTTGCC AGCCACACAC GCTTCTCCTC GGTGGACAGC 
GCCAAGATCT CTGAGAGCAC AGTCTTTTCT TCACAAGAGG ATGAGGAGGA GGAAAACAGT 
GCATTCGAGT CGGTACCTGA CTCGGTGCAA AGCCCAGAGC TAGATCCCGA GTCTACAAAT 
GGGGCTGGGC CTTGGCAGGA TGAACTGGCT GCCCCTGGTG GGAATGCAGC AAGAACTACT 
GAAGGTCTGG ACTCCCCCAT GGCAGGCCCT AGCAATAGGA GAGAAGGTGA CTGTCCTATG 
CTCCATAATT CCCAGCCAAT AAGCCAGCTT CCTTCCTTGA GGCCTGAACA TCATCACTAC 
CCAACAATCG ATGAGCCTCT TCCACCAAAC TGGGAAGCTC GAATCGACAG CCATGGACGG 
GTCTTTTATG TGGACCATGT AAATCGCACA ACCACCTGGC AGCGACCATC AATGGCGCCT 
ACCCCAGATG GAATGATCAG ATCCGGGTCT GTCCACCAGA TGGAGCAGCT TAACAGAAGG 
TATCAAAACA TCCAGAGAAC CATTGCAACA GAGAGATCTG AAGAAGATTC TGGCAACCAA 
AGCTGTGAGC AAATACCAGA CGGAGGAGCT GGAGGTGGAG GGAGTGACTC GGAAGCAGAA 
TCTTCCCAAT CAAACTTAGA TCTGAGGAGA GAAGGGTCAC TTTCTCCTGT CAACTCACAG 
AAGGTCACCT TGCTGCTGCA GTCTCCAGCA GTCAAGTTCA TCACCAATCC TGAGTTCTTC 
ACTGTGTTGC ATGCCAATTA TAGTGCCTAC AGGGTTTTTA CAAGTAGCAC TTGCCTGAAG 
CATATGATTT TGAAAGTCCG AAGAGATGCT CGCAATTTTG AACGCTACCA GCACAACCGG 
GACCTGGTGA ATTTCATCAA CATGTTTGCA GATACTCGGC TGGAACTGCC CCGGGGTTGG 
GAGATCAAAA CTGATCATCA GGGAAAGTCT TTCTTTGTGG ACCACAACAG TCGGGCTACT 
ACTTTCATTG ATCCCCGAAT CCCTCTTCAG AATGGCCGCC TTCCCAATCA TCTAACCCAC 
CGACAGCACC TCCAGAGGCT CAGGAGTTAC AGTGCTGGTG AGGCCTCAGA AGTCTCTAGA 
AACAGAGGAG CATCTTTGCT GGCCAGACCA GGACACAGCC TAATAGCTGC TATTCGGAGC 
CAACATCAAC ACGAGTCCTT GCCACTGGCA TACAATGACA AGATTGTGGC ATTTCTTCGA 
CAGCCAAACA TTTTTGAAAT GCTACAAGAA CGTCAGCCAA GCTTGGCAAG AAACCACACA 
CTCAGGGAGA AAATCCATTA TATTCGGACA GAGGGCAATC ATGGACTTGA GAAATTGTCC 
TGTGATGCAG ATCTAGTCAT TTTGCTAAGT CTCTTTGAAG AAGAGATCAT GTCCTATGTC 
CCCCTGCAAT CTGCCTTTCA CCCTGGATAT AGCTTCTCTC CCCGCTGTTC ACCCTGTTCC 
TCACCCCAGA ACTCCCCAGG TTTACAGAGA GCCAGTGCCA GGGCCCCTTC ACCGTACCGA 
AGGGATTTTG AAGCTAAGCT CCGCAATTTC TACCGAAAAC TGGAAGCCAA AGGATTTGGG 
CAGGGTCCGG GTAAAATTAA GCTCATCATC CGTCGAGACC ACTTGTTGGA GGGGACCTTC 
AATCAGGTGA TGGCATACTC TCGGAAGGAG CTCCAGAGAA ACAAGCTCTA CATCACCTTT 
GTTGGAGAGG AGGGGCTGGA CTACAGTGGC CCTTCTCGAG AGTTCTTCTT CCTGTTGTCT 
CAGGAGCTCT TCAACCCTTA CTATGGACTC TTTGAGTATT CTGCAAATGA CACTTACACA 
GTGCAGATCA GCCCCATGTC GGCGTTTGTA GAAAATTATC TCGAATGGTT TAGATTCAGC 
GGCCGTATCC TGGGCCTAGC TCTGATCCAC CAGTACCTCT TGGATGCTTT CTTCACCAGA 
CCCTTCTATA AGGGACTCCT GAAGCTGCCA TGCGATCTGA GTGACCTGGA ATACTTGGAT 
GAGGAATTCC ACCAGAGTTT ACAGTGGATG AAGGACAACA ACATCACAGA TATTCTTGAC 
CTCACTTTCA CTGTTAACGA AGAAGTTTTT GGACAGGTAA CAGAAAGAGA GTTGAAGTCT 
GGAGGTGCCA ACACGCAGGT GACTGAGAAG AACAAGAAGG AGTACATCGA GAGGATGGTG 
AAGTGGCGTG TAGAGCGTGG TGTGGTACAG CAGACCGAGG CATTGGTCAG GGGTTTCTAT 
GAGGTTGTAG ACTCAAGGCT TGTCTCAGTG TTTGACGCGA GGGAGCTGGA GCTGGTAATC 
GCGGGAACTG CTGAAATCGA CCTGAATGAC TGGAGGAATA ACACTGAGTA CCGGGGAGGT 
TATCATGATG GCCACCTTGT GATCCGCTGG TTCTGGGCTG CAGTGGAGCG CTTCAATAAT 
GAGCAGAGAT TGAGATTACT TCAGTTTGTG ACAGGAACGT CGAGTGTTCC CTATGAAGGC 
TTTGCAGCTC TGCGGGGGAG CAATGGGCTT CGAAGGTTCT GCATAGAGAA GTGGGGGAAG 
ATTACATCTC TTCCCAGGGC ACATACATGC TTCAACCGAC TGGATCTCCC ACCGTATCCT 
TCATACTCCA CATTGTATGA AAAGTTGTTA ACAGCAGTTG AAGAAACCAG CACCTTTGGA 
CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_017600485.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455974.1 Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X1, mRNA.

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CloneID	ORa59015
Clone ID Related Accession (Same CDS sequence)	XM_017600485.1
Accession Version	XM_017600485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGCTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CATCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAGCCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GGCATCACCA TCCCTCGCTC TACCAGTGAC ACTGACCTGG TCACCTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCT ATAGGGTATT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCCGGA GACTGGATTG GGATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGATC TCACAGAGGC 
CAGATCATTT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGCGGGGCC CTGAGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CGGCTCCTAT TTTTAAAGGT ATTGGCTCTG AGGAGACTGT GCAAAGTCAA 
GGAAACAGGA GGCTCATCAG CTTCTCTCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAACCTGG TAAACATAGC 
ATCTTCCCGG CCCTCCCTCA TCATGGACAG GAACGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC TGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCAGTTCA GAGGCTACTG GAGAGACATG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACT GACCATGTGA GTGGACATCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCACGCAGAC GATGAAGAGA TCTCTCTGAA TGCTGAGCCT 
GAGTCAGCGG AAGTGCAGGA CAGTGTCATG AATAGTGTGG TGGAAAACAG CAGTGGGGAG 
CCTCCCGGTG ATGCTGCAGA ATTCCTCAAG GACTTGAAGC CAGAGGCACC GAGTGAAGAG 
GATGGAGTGC GCGGCTCAGA AAATGAAAAC CGAGAGCATG CCAGGCCAGT CGAGGAAGCT 
GCGGGTGCTA TGGAGACCAG AGATGATAGC AAGTTCTCTG AAGGACCGGA AGAGGTTGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACTC TGAGCGCAGA AGAAGTGGCA 
GAGCGACTTC CCCTGGGTGA GGGTTGCCCA CCCTCGCTGT TGCCAGAAGA GAGTACAGCT 
CTAGGCAGCA AGGCAGAAGA GGAAGCAATT CCAGAAAATG GAGCGGGAGA GGAAGAGATG 
CAGGAGAAGG GGAAAGATGA GGAGGAAGAA GAGGGTGTTT CTGCCCTGGA GCAAGGAGAG 
CCTGGACTTG AGCTGAGGGC CTCCGTGAGA AAAAAAAGCA GGCCATGTTC CTTACCTGTA 
TCCGAGCTTG AAACAGTGAT CGCATCTGCC TGTGGAGAGG CTGAGACCCC AAGGACCCAC 
TACATCCGCA TCCACACCCT ACTGCACAGC ATGCCCTCGG CCCAGAGGGG AAGCACCACA 
GAGGAGGAGG ATGCCATGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAACAAT 
GGCGCTACTC TCTGCATGGC ATCCTCTGAC TGCTCTGGGG GCCATTTCTC CAATTTATCT 
AAAAGCATTG GCGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGTAGC 
CCCTCATGCT ATAGCACATC GTGCTACAGC AGCTCCTGTT ATAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGCAA CAATCGGTTT GCCAGCCACA CACGCTTCTC CTCGGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA GGAGGAAAAC 
AGTGCATTCG AGTCGGTACC TGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GGCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAACT 
ACTGAAGGTC TGGACTCCCC CATGGCAGGC CCTAGCAATA GGAGAGAAGG TGACTGTCCT 
ATGCTCCATA ATTCCCAGCC AATAAGCCAG CTTCCTTCCT TGAGGCCTGA ACATCATCAC 
TACCCAACAA TCGATGAGCC TCTTCCACCA AACTGGGAAG CTCGAATCGA CAGCCATGGA 
CGGGTCTTTT ATGTGGACCA TGTAAATCGC ACAACCACCT GGCAGCGACC ATCAATGGCG 
CCTACCCCAG ATGGAATGAT CAGATCCGGG TCTGTCCACC AGATGGAGCA GCTTAACAGA 
AGGTATCAAA ACATCCAGAG AACCATTGCA ACAGAGAGAT CTGAAGAAGA TTCTGGCAAC 
CAAAGCTGTG AGCAAATACC AGACGGAGGA GCTGGAGGTG GAGGGAGTGA CTCGGAAGCA 
GAATCTTCCC AATCAAACTT AGATCTGAGG AGAGAAGGGT CACTTTCTCC TGTCAACTCA 
CAGAAGGTCA CCTTGCTGCT GCAGTCTCCA GCAGTCAAGT TCATCACCAA TCCTGAGTTC 
TTCACTGTGT TGCATGCCAA TTATAGTGCC TACAGGGTTT TTACAAGTAG CACTTGCCTG 
AAGCATATGA TTTTGAAAGT CCGAAGAGAT GCTCGCAATT TTGAACGCTA CCAGCACAAC 
CGGGACCTGG TGAATTTCAT CAACATGTTT GCAGATACTC GGCTGGAACT GCCCCGGGGT 
TGGGAGATCA AAACTGATCA TCAGGGAAAG TCTTTCTTTG TGGACCACAA CAGTCGGGCT 
ACTACTTTCA TTGATCCCCG AATCCCTCTT CAGAATGGCC GCCTTCCCAA TCATCTAACC 
CACCGACAGC ACCTCCAGAG GCTCAGGAGT TACAGTGCTG GTGAGGCCTC AGAAGTCTCT 
AGAAACAGAG GAGCATCTTT GCTGGCCAGA CCAGGACACA GCCTAATAGC TGCTATTCGG 
AGCCAACATC AACACGAGTC CTTGCCACTG GCATACAATG ACAAGATTGT GGCATTTCTT 
CGACAGCCAA ACATTTTTGA AATGCTACAA GAACGTCAGC CAAGCTTGGC AAGAAACCAC 
ACACTCAGGG AGAAAATCCA TTATATTCGG ACAGAGGGCA ATCATGGACT TGAGAAATTG 
TCCTGTGATG CAGATCTAGT CATTTTGCTA AGTCTCTTTG AAGAAGAGAT CATGTCCTAT 
GTCCCCCTGC AATCTGCCTT TCACCCTGGA TATAGCTTCT CTCCCCGCTG TTCACCCTGT 
TCCTCACCCC AGAACTCCCC AGGTTTACAG AGAGCCAGTG CCAGGGCCCC TTCACCGTAC 
CGAAGGGATT TTGAAGCTAA GCTCCGCAAT TTCTACCGAA AACTGGAAGC CAAAGGATTT 
GGGCAGGGTC CGGGTAAAAT TAAGCTCATC ATCCGTCGAG ACCACTTGTT GGAGGGGACC 
TTCAATCAGG TGATGGCATA CTCTCGGAAG GAGCTCCAGA GAAACAAGCT CTACATCACC 
TTTGTTGGAG AGGAGGGGCT GGACTACAGT GGCCCTTCTC GAGAGTTCTT CTTCCTGTTG 
TCTCAGGAGC TCTTCAACCC TTACTATGGA CTCTTTGAGT ATTCTGCAAA TGACACTTAC 
ACAGTGCAGA TCAGCCCCAT GTCGGCGTTT GTAGAAAATT ATCTCGAATG GTTTAGATTC 
AGCGGCCGTA TCCTGGGCCT AGCTCTGATC CACCAGTACC TCTTGGATGC TTTCTTCACC 
AGACCCTTCT ATAAGGGACT CCTGAAGCTG CCATGCGATC TGAGTGACCT GGAATACTTG 
GATGAGGAAT TCCACCAGAG TTTACAGTGG ATGAAGGACA ACAACATCAC AGATATTCTT 
GACCTCACTT TCACTGTTAA CGAAGAAGTT TTTGGACAGG TAACAGAAAG AGAGTTGAAG 
TCTGGAGGTG CCAACACGCA GGTGACTGAG AAGAACAAGA AGGAGTACAT CGAGAGGATG 
GTGAAGTGGC GTGTAGAGCG TGGTGTGGTA CAGCAGACCG AGGCATTGGT CAGGGGTTTC 
TATGAGGTTG TAGACTCAAG GCTTGTCTCA GTGTTTGACG CGAGGGAGCT GGAGCTGGTA 
ATCGCGGGAA CTGCTGAAAT CGACCTGAAT GACTGGAGGA ATAACACTGA GTACCGGGGA 
GGTTATCATG ATGGCCACCT TGTGATCCGC TGGTTCTGGG CTGCAGTGGA GCGCTTCAAT 
AATGAGCAGA GATTGAGATT ACTTCAGTTT GTGACAGGAA CGTCGAGTGT TCCCTATGAA 
GGCTTTGCAG CTCTGCGGGG GAGCAATGGG CTTCGAAGGT TCTGCATAGA GAAGTGGGGG 
AAGATTACAT CTCTTCCCAG GGCACATACA TGCTTCAACC GACTGGATCT CCCACCGTAT 
CCTTCATACT CCACATTGTA TGAAAAGTTG TTAACAGCAG TTGAAGAAAC CAGCACCTTT 
GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455974.1
CDS	37..4848
Translation	MICAQLEAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDGITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGYSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETVQSQGNRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGHLQFRFEITSSIHADDEEISLNAEPESAEVQDSVMNSVVENSSGEPPGDAAEFLKDLKPEAPSEEDGVRGSENENREHARPVEEAAGAMETRDDSKFSEGPEEVGELQDPEQHDTQPTLSAEEVAERLPLGEGCPPSLLPEESTALGSKAEEEAIPENGAGEEEMQEKGKDEEEEEGVSALEQGEPGLELRASVRKKSRPCSLPVSELETVIASACGEAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDAMEEESTLKESSEKDGLSEVDTIAADPQSMEDGENNGATLCMASSDCSGGHFSNLSKSIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARTTEGLDSPMAGPSNRREGDCPMLHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTIATERSEEDSGNQSCEQIPDGGAGGGGSDSEAESSQSNLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSTLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017600485.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600485.1

ORF Insert Sequence:

ATGATCTGTG CCCAGCTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CATCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAGCCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GGCATCACCA TCCCTCGCTC TACCAGTGAC ACTGACCTGG TCACCTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCT ATAGGGTATT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCCGGA GACTGGATTG GGATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGATC TCACAGAGGC 
CAGATCATTT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGCGGGGCC CTGAGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CGGCTCCTAT TTTTAAAGGT ATTGGCTCTG AGGAGACTGT GCAAAGTCAA 
GGAAACAGGA GGCTCATCAG CTTCTCTCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAACCTGG TAAACATAGC 
ATCTTCCCGG CCCTCCCTCA TCATGGACAG GAACGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC TGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCAGTTCA GAGGCTACTG GAGAGACATG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACT GACCATGTGA GTGGACATCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCACGCAGAC GATGAAGAGA TCTCTCTGAA TGCTGAGCCT 
GAGTCAGCGG AAGTGCAGGA CAGTGTCATG AATAGTGTGG TGGAAAACAG CAGTGGGGAG 
CCTCCCGGTG ATGCTGCAGA ATTCCTCAAG GACTTGAAGC CAGAGGCACC GAGTGAAGAG 
GATGGAGTGC GCGGCTCAGA AAATGAAAAC CGAGAGCATG CCAGGCCAGT CGAGGAAGCT 
GCGGGTGCTA TGGAGACCAG AGATGATAGC AAGTTCTCTG AAGGACCGGA AGAGGTTGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACTC TGAGCGCAGA AGAAGTGGCA 
GAGCGACTTC CCCTGGGTGA GGGTTGCCCA CCCTCGCTGT TGCCAGAAGA GAGTACAGCT 
CTAGGCAGCA AGGCAGAAGA GGAAGCAATT CCAGAAAATG GAGCGGGAGA GGAAGAGATG 
CAGGAGAAGG GGAAAGATGA GGAGGAAGAA GAGGGTGTTT CTGCCCTGGA GCAAGGAGAG 
CCTGGACTTG AGCTGAGGGC CTCCGTGAGA AAAAAAAGCA GGCCATGTTC CTTACCTGTA 
TCCGAGCTTG AAACAGTGAT CGCATCTGCC TGTGGAGAGG CTGAGACCCC AAGGACCCAC 
TACATCCGCA TCCACACCCT ACTGCACAGC ATGCCCTCGG CCCAGAGGGG AAGCACCACA 
GAGGAGGAGG ATGCCATGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAACAAT 
GGCGCTACTC TCTGCATGGC ATCCTCTGAC TGCTCTGGGG GCCATTTCTC CAATTTATCT 
AAAAGCATTG GCGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGTAGC 
CCCTCATGCT ATAGCACATC GTGCTACAGC AGCTCCTGTT ATAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGCAA CAATCGGTTT GCCAGCCACA CACGCTTCTC CTCGGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA GGAGGAAAAC 
AGTGCATTCG AGTCGGTACC TGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GGCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAACT 
ACTGAAGGTC TGGACTCCCC CATGGCAGGC CCTAGCAATA GGAGAGAAGG TGACTGTCCT 
ATGCTCCATA ATTCCCAGCC AATAAGCCAG CTTCCTTCCT TGAGGCCTGA ACATCATCAC 
TACCCAACAA TCGATGAGCC TCTTCCACCA AACTGGGAAG CTCGAATCGA CAGCCATGGA 
CGGGTCTTTT ATGTGGACCA TGTAAATCGC ACAACCACCT GGCAGCGACC ATCAATGGCG 
CCTACCCCAG ATGGAATGAT CAGATCCGGG TCTGTCCACC AGATGGAGCA GCTTAACAGA 
AGGTATCAAA ACATCCAGAG AACCATTGCA ACAGAGAGAT CTGAAGAAGA TTCTGGCAAC 
CAAAGCTGTG AGCAAATACC AGACGGAGGA GCTGGAGGTG GAGGGAGTGA CTCGGAAGCA 
GAATCTTCCC AATCAAACTT AGATCTGAGG AGAGAAGGGT CACTTTCTCC TGTCAACTCA 
CAGAAGGTCA CCTTGCTGCT GCAGTCTCCA GCAGTCAAGT TCATCACCAA TCCTGAGTTC 
TTCACTGTGT TGCATGCCAA TTATAGTGCC TACAGGGTTT TTACAAGTAG CACTTGCCTG 
AAGCATATGA TTTTGAAAGT CCGAAGAGAT GCTCGCAATT TTGAACGCTA CCAGCACAAC 
CGGGACCTGG TGAATTTCAT CAACATGTTT GCAGATACTC GGCTGGAACT GCCCCGGGGT 
TGGGAGATCA AAACTGATCA TCAGGGAAAG TCTTTCTTTG TGGACCACAA CAGTCGGGCT 
ACTACTTTCA TTGATCCCCG AATCCCTCTT CAGAATGGCC GCCTTCCCAA TCATCTAACC 
CACCGACAGC ACCTCCAGAG GCTCAGGAGT TACAGTGCTG GTGAGGCCTC AGAAGTCTCT 
AGAAACAGAG GAGCATCTTT GCTGGCCAGA CCAGGACACA GCCTAATAGC TGCTATTCGG 
AGCCAACATC AACACGAGTC CTTGCCACTG GCATACAATG ACAAGATTGT GGCATTTCTT 
CGACAGCCAA ACATTTTTGA AATGCTACAA GAACGTCAGC CAAGCTTGGC AAGAAACCAC 
ACACTCAGGG AGAAAATCCA TTATATTCGG ACAGAGGGCA ATCATGGACT TGAGAAATTG 
TCCTGTGATG CAGATCTAGT CATTTTGCTA AGTCTCTTTG AAGAAGAGAT CATGTCCTAT 
GTCCCCCTGC AATCTGCCTT TCACCCTGGA TATAGCTTCT CTCCCCGCTG TTCACCCTGT 
TCCTCACCCC AGAACTCCCC AGGTTTACAG AGAGCCAGTG CCAGGGCCCC TTCACCGTAC 
CGAAGGGATT TTGAAGCTAA GCTCCGCAAT TTCTACCGAA AACTGGAAGC CAAAGGATTT 
GGGCAGGGTC CGGGTAAAAT TAAGCTCATC ATCCGTCGAG ACCACTTGTT GGAGGGGACC 
TTCAATCAGG TGATGGCATA CTCTCGGAAG GAGCTCCAGA GAAACAAGCT CTACATCACC 
TTTGTTGGAG AGGAGGGGCT GGACTACAGT GGCCCTTCTC GAGAGTTCTT CTTCCTGTTG 
TCTCAGGAGC TCTTCAACCC TTACTATGGA CTCTTTGAGT ATTCTGCAAA TGACACTTAC 
ACAGTGCAGA TCAGCCCCAT GTCGGCGTTT GTAGAAAATT ATCTCGAATG GTTTAGATTC 
AGCGGCCGTA TCCTGGGCCT AGCTCTGATC CACCAGTACC TCTTGGATGC TTTCTTCACC 
AGACCCTTCT ATAAGGGACT CCTGAAGCTG CCATGCGATC TGAGTGACCT GGAATACTTG 
GATGAGGAAT TCCACCAGAG TTTACAGTGG ATGAAGGACA ACAACATCAC AGATATTCTT 
GACCTCACTT TCACTGTTAA CGAAGAAGTT TTTGGACAGG TAACAGAAAG AGAGTTGAAG 
TCTGGAGGTG CCAACACGCA GGTGACTGAG AAGAACAAGA AGGAGTACAT CGAGAGGATG 
GTGAAGTGGC GTGTAGAGCG TGGTGTGGTA CAGCAGACCG AGGCATTGGT CAGGGGTTTC 
TATGAGGTTG TAGACTCAAG GCTTGTCTCA GTGTTTGACG CGAGGGAGCT GGAGCTGGTA 
ATCGCGGGAA CTGCTGAAAT CGACCTGAAT GACTGGAGGA ATAACACTGA GTACCGGGGA 
GGTTATCATG ATGGCCACCT TGTGATCCGC TGGTTCTGGG CTGCAGTGGA GCGCTTCAAT 
AATGAGCAGA GATTGAGATT ACTTCAGTTT GTGACAGGAA CGTCGAGTGT TCCCTATGAA 
GGCTTTGCAG CTCTGCGGGG GAGCAATGGG CTTCGAAGGT TCTGCATAGA GAAGTGGGGG 
AAGATTACAT CTCTTCCCAG GGCACATACA TGCTTCAACC GACTGGATCT CCCACCGTAT 
CCTTCATACT CCACATTGTA TGAAAAGTTG TTAACAGCAG TTGAAGAAAC CAGCACCTTT 
GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_017600487.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455976.1 Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X4, mRNA.

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CloneID	ORa59016
Clone ID Related Accession (Same CDS sequence)	XM_017600487.1
Accession Version	XM_017600487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGCTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CATCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAGCCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GGCATCACCA TCCCTCGCTC TACCAGTGAC ACTGACCTGG TCACCTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCT ATAGGGTATT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCCGGA GACTGGATTG GGATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGATC TCACAGAGGC 
CAGATCATTT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGCGGGGCC CTGAGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CGGCTCCTAT TTTTAAAGGT ATTGGCTCTG AGGAGACTGT GCAAAGTCAA 
GGAAACAGGA GGCTCATCAG CTTCTCTCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAACCTGG TAAACATAGC 
ATCTTCCCGG CCCTCCCTCA TCATGGACAG GAACGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC TGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCAGTTCA GAGGCTACTG GAGAGACATG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACT GACCATGTGA GTGGACATCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCACGCAGAC GATGAAGAGA TCTCTCTGAA TGCTGAGCCT 
GAGTCAGCGG AAGTGCAGGA CAGTGTCATG AATAGTGTGG TGGAAAACAG CAGTGGGGAG 
CCTCCCGGTG ATGCTGCAGA ATTCCTCAAG GACTTGAAGC CAGAGGCACC GAGTGAAGAG 
GATGGAGTGC GCGGCTCAGA AAATGAAAAC CGAGAGCATG CCAGGCCAGT CGAGGAAGCT 
GCGGGTGCTA TGGAGACCAG AGATGATAGC AAGTTCTCTG AAGGACCGGA AGAGGTTGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACTC TGAGCGCAGA AGAAGTGGCA 
GAGCGACTTC CCCTGGGTGA GGGTTGCCCA CCCTCGCTGT TGCCAGAAGA GAGTACAGCT 
CTAGGCAGCA AGGCAGAAGA GGAAGCAATT CCAGAAAATG GAGCGGGAGA GGAAGAGATG 
CAGGAGAAGG GGAAAGATGA GGAGGAAGAA GAGGGTGTTT CTGCCCTGGA GCAAGGAGAG 
CCTGGACTTG AGCTGAGGGC CTCCGTGAGA AAAAAAAGCA GGCCATGTTC CTTACCTGTA 
TCCGAGCTTG AAACAGTGAT CGCATCTGCC TGTGGAGAGG CTGAGACCCC AAGGACCCAC 
TACATCCGCA TCCACACCCT ACTGCACAGC ATGCCCTCGG CCCAGAGGGG AAGCACCACA 
GAGGAGGAGG ATGCCATGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAACAAT 
GGCGCTACTC TCTGCATGGC ATCCTCTGAC TGCTCTGGGG GCCATTTCTC CAATTTATCT 
AAAAGCATTG GCGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGTAGC 
CCCTCATGCT ATAGCACATC GTGCTACAGC AGCTCCTGTT ATAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGCAA CAATCGGTTT GCCAGCCACA CACGCTTCTC CTCGGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA GGAGGAAAAC 
AGTGCATTCG AGTCGGTACC TGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GGCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAACT 
ACTGAAGGTG ACTGTCCTAT GCTCCATAAT TCCCAGCCAA TAAGCCAGCT TCCTTCCTTG 
AGGCCTGAAC ATCATCACTA CCCAACAATC GATGAGCCTC TTCCACCAAA CTGGGAAGCT 
CGAATCGACA GCCATGGACG GGTCTTTTAT GTGGACCATG TAAATCGCAC AACCACCTGG 
CAGCGACCAT CAATGGCGCC TACCCCAGAT GGAATGATCA GATCCGGGTC TGTCCACCAG 
ATGGAGCAGC TTAACAGAAG GTATCAAAAC ATCCAGAGAA CCATTGCAAC AGAGAGATCT 
GAAGAAGATT CTGGCAACCA AAGCTGTGAG CAAATACCAG ACGGAGGAGC TGGAGGTGGA 
GGGAGTGACT CGGAAGCAGA ATCTTCCCAA TCAAACTTAG ATCTGAGGAG AGAAGGGTCA 
CTTTCTCCTG TCAACTCACA GAAGGTCACC TTGCTGCTGC AGTCTCCAGC AGTCAAGTTC 
ATCACCAATC CTGAGTTCTT CACTGTGTTG CATGCCAATT ATAGTGCCTA CAGGGTTTTT 
ACAAGTAGCA CTTGCCTGAA GCATATGATT TTGAAAGTCC GAAGAGATGC TCGCAATTTT 
GAACGCTACC AGCACAACCG GGACCTGGTG AATTTCATCA ACATGTTTGC AGATACTCGG 
CTGGAACTGC CCCGGGGTTG GGAGATCAAA ACTGATCATC AGGGAAAGTC TTTCTTTGTG 
GACCACAACA GTCGGGCTAC TACTTTCATT GATCCCCGAA TCCCTCTTCA GAATGGCCGC 
CTTCCCAATC ATCTAACCCA CCGACAGCAC CTCCAGAGGC TCAGGAGTTA CAGTGCTGGT 
GAGGCCTCAG AAGTCTCTAG AAACAGAGGA GCATCTTTGC TGGCCAGACC AGGACACAGC 
CTAATAGCTG CTATTCGGAG CCAACATCAA CACGAGTCCT TGCCACTGGC ATACAATGAC 
AAGATTGTGG CATTTCTTCG ACAGCCAAAC ATTTTTGAAA TGCTACAAGA ACGTCAGCCA 
AGCTTGGCAA GAAACCACAC ACTCAGGGAG AAAATCCATT ATATTCGGAC AGAGGGCAAT 
CATGGACTTG AGAAATTGTC CTGTGATGCA GATCTAGTCA TTTTGCTAAG TCTCTTTGAA 
GAAGAGATCA TGTCCTATGT CCCCCTGCAA TCTGCCTTTC ACCCTGGATA TAGCTTCTCT 
CCCCGCTGTT CACCCTGTTC CTCACCCCAG AACTCCCCAG GTTTACAGAG AGCCAGTGCC 
AGGGCCCCTT CACCGTACCG AAGGGATTTT GAAGCTAAGC TCCGCAATTT CTACCGAAAA 
CTGGAAGCCA AAGGATTTGG GCAGGGTCCG GGTAAAATTA AGCTCATCAT CCGTCGAGAC 
CACTTGTTGG AGGGGACCTT CAATCAGGTG ATGGCATACT CTCGGAAGGA GCTCCAGAGA 
AACAAGCTCT ACATCACCTT TGTTGGAGAG GAGGGGCTGG ACTACAGTGG CCCTTCTCGA 
GAGTTCTTCT TCCTGTTGTC TCAGGAGCTC TTCAACCCTT ACTATGGACT CTTTGAGTAT 
TCTGCAAATG ACACTTACAC AGTGCAGATC AGCCCCATGT CGGCGTTTGT AGAAAATTAT 
CTCGAATGGT TTAGATTCAG CGGCCGTATC CTGGGCCTAG CTCTGATCCA CCAGTACCTC 
TTGGATGCTT TCTTCACCAG ACCCTTCTAT AAGGGACTCC TGAAGCTGCC ATGCGATCTG 
AGTGACCTGG AATACTTGGA TGAGGAATTC CACCAGAGTT TACAGTGGAT GAAGGACAAC 
AACATCACAG ATATTCTTGA CCTCACTTTC ACTGTTAACG AAGAAGTTTT TGGACAGGTA 
ACAGAAAGAG AGTTGAAGTC TGGAGGTGCC AACACGCAGG TGACTGAGAA GAACAAGAAG 
GAGTACATCG AGAGGATGGT GAAGTGGCGT GTAGAGCGTG GTGTGGTACA GCAGACCGAG 
GCATTGGTCA GGGGTTTCTA TGAGGTTGTA GACTCAAGGC TTGTCTCAGT GTTTGACGCG 
AGGGAGCTGG AGCTGGTAAT CGCGGGAACT GCTGAAATCG ACCTGAATGA CTGGAGGAAT 
AACACTGAGT ACCGGGGAGG TTATCATGAT GGCCACCTTG TGATCCGCTG GTTCTGGGCT 
GCAGTGGAGC GCTTCAATAA TGAGCAGAGA TTGAGATTAC TTCAGTTTGT GACAGGAACG 
TCGAGTGTTC CCTATGAAGG CTTTGCAGCT CTGCGGGGGA GCAATGGGCT TCGAAGGTTC 
TGCATAGAGA AGTGGGGGAA GATTACATCT CTTCCCAGGG CACATACATG CTTCAACCGA 
CTGGATCTCC CACCGTATCC TTCATACTCC ACATTGTATG AAAAGTTGTT AACAGCAGTT 
GAAGAAACCA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455976.1
CDS	37..4806
Translation	MICAQLEAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDGITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGYSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETVQSQGNRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGHLQFRFEITSSIHADDEEISLNAEPESAEVQDSVMNSVVENSSGEPPGDAAEFLKDLKPEAPSEEDGVRGSENENREHARPVEEAAGAMETRDDSKFSEGPEEVGELQDPEQHDTQPTLSAEEVAERLPLGEGCPPSLLPEESTALGSKAEEEAIPENGAGEEEMQEKGKDEEEEEGVSALEQGEPGLELRASVRKKSRPCSLPVSELETVIASACGEAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDAMEEESTLKESSEKDGLSEVDTIAADPQSMEDGENNGATLCMASSDCSGGHFSNLSKSIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARTTEGDCPMLHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTIATERSEEDSGNQSCEQIPDGGAGGGGSDSEAESSQSNLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSTLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017600487.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600487.1

ORF Insert Sequence:

ATGATCTGTG CCCAGCTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CATCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAGCCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GGCATCACCA TCCCTCGCTC TACCAGTGAC ACTGACCTGG TCACCTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCT ATAGGGTATT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCCGGA GACTGGATTG GGATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGATC TCACAGAGGC 
CAGATCATTT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGCGGGGCC CTGAGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CGGCTCCTAT TTTTAAAGGT ATTGGCTCTG AGGAGACTGT GCAAAGTCAA 
GGAAACAGGA GGCTCATCAG CTTCTCTCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAACCTGG TAAACATAGC 
ATCTTCCCGG CCCTCCCTCA TCATGGACAG GAACGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC TGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCAGTTCA GAGGCTACTG GAGAGACATG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACT GACCATGTGA GTGGACATCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCACGCAGAC GATGAAGAGA TCTCTCTGAA TGCTGAGCCT 
GAGTCAGCGG AAGTGCAGGA CAGTGTCATG AATAGTGTGG TGGAAAACAG CAGTGGGGAG 
CCTCCCGGTG ATGCTGCAGA ATTCCTCAAG GACTTGAAGC CAGAGGCACC GAGTGAAGAG 
GATGGAGTGC GCGGCTCAGA AAATGAAAAC CGAGAGCATG CCAGGCCAGT CGAGGAAGCT 
GCGGGTGCTA TGGAGACCAG AGATGATAGC AAGTTCTCTG AAGGACCGGA AGAGGTTGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACTC TGAGCGCAGA AGAAGTGGCA 
GAGCGACTTC CCCTGGGTGA GGGTTGCCCA CCCTCGCTGT TGCCAGAAGA GAGTACAGCT 
CTAGGCAGCA AGGCAGAAGA GGAAGCAATT CCAGAAAATG GAGCGGGAGA GGAAGAGATG 
CAGGAGAAGG GGAAAGATGA GGAGGAAGAA GAGGGTGTTT CTGCCCTGGA GCAAGGAGAG 
CCTGGACTTG AGCTGAGGGC CTCCGTGAGA AAAAAAAGCA GGCCATGTTC CTTACCTGTA 
TCCGAGCTTG AAACAGTGAT CGCATCTGCC TGTGGAGAGG CTGAGACCCC AAGGACCCAC 
TACATCCGCA TCCACACCCT ACTGCACAGC ATGCCCTCGG CCCAGAGGGG AAGCACCACA 
GAGGAGGAGG ATGCCATGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAACAAT 
GGCGCTACTC TCTGCATGGC ATCCTCTGAC TGCTCTGGGG GCCATTTCTC CAATTTATCT 
AAAAGCATTG GCGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGTAGC 
CCCTCATGCT ATAGCACATC GTGCTACAGC AGCTCCTGTT ATAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGCAA CAATCGGTTT GCCAGCCACA CACGCTTCTC CTCGGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA GGAGGAAAAC 
AGTGCATTCG AGTCGGTACC TGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GGCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAACT 
ACTGAAGGTG ACTGTCCTAT GCTCCATAAT TCCCAGCCAA TAAGCCAGCT TCCTTCCTTG 
AGGCCTGAAC ATCATCACTA CCCAACAATC GATGAGCCTC TTCCACCAAA CTGGGAAGCT 
CGAATCGACA GCCATGGACG GGTCTTTTAT GTGGACCATG TAAATCGCAC AACCACCTGG 
CAGCGACCAT CAATGGCGCC TACCCCAGAT GGAATGATCA GATCCGGGTC TGTCCACCAG 
ATGGAGCAGC TTAACAGAAG GTATCAAAAC ATCCAGAGAA CCATTGCAAC AGAGAGATCT 
GAAGAAGATT CTGGCAACCA AAGCTGTGAG CAAATACCAG ACGGAGGAGC TGGAGGTGGA 
GGGAGTGACT CGGAAGCAGA ATCTTCCCAA TCAAACTTAG ATCTGAGGAG AGAAGGGTCA 
CTTTCTCCTG TCAACTCACA GAAGGTCACC TTGCTGCTGC AGTCTCCAGC AGTCAAGTTC 
ATCACCAATC CTGAGTTCTT CACTGTGTTG CATGCCAATT ATAGTGCCTA CAGGGTTTTT 
ACAAGTAGCA CTTGCCTGAA GCATATGATT TTGAAAGTCC GAAGAGATGC TCGCAATTTT 
GAACGCTACC AGCACAACCG GGACCTGGTG AATTTCATCA ACATGTTTGC AGATACTCGG 
CTGGAACTGC CCCGGGGTTG GGAGATCAAA ACTGATCATC AGGGAAAGTC TTTCTTTGTG 
GACCACAACA GTCGGGCTAC TACTTTCATT GATCCCCGAA TCCCTCTTCA GAATGGCCGC 
CTTCCCAATC ATCTAACCCA CCGACAGCAC CTCCAGAGGC TCAGGAGTTA CAGTGCTGGT 
GAGGCCTCAG AAGTCTCTAG AAACAGAGGA GCATCTTTGC TGGCCAGACC AGGACACAGC 
CTAATAGCTG CTATTCGGAG CCAACATCAA CACGAGTCCT TGCCACTGGC ATACAATGAC 
AAGATTGTGG CATTTCTTCG ACAGCCAAAC ATTTTTGAAA TGCTACAAGA ACGTCAGCCA 
AGCTTGGCAA GAAACCACAC ACTCAGGGAG AAAATCCATT ATATTCGGAC AGAGGGCAAT 
CATGGACTTG AGAAATTGTC CTGTGATGCA GATCTAGTCA TTTTGCTAAG TCTCTTTGAA 
GAAGAGATCA TGTCCTATGT CCCCCTGCAA TCTGCCTTTC ACCCTGGATA TAGCTTCTCT 
CCCCGCTGTT CACCCTGTTC CTCACCCCAG AACTCCCCAG GTTTACAGAG AGCCAGTGCC 
AGGGCCCCTT CACCGTACCG AAGGGATTTT GAAGCTAAGC TCCGCAATTT CTACCGAAAA 
CTGGAAGCCA AAGGATTTGG GCAGGGTCCG GGTAAAATTA AGCTCATCAT CCGTCGAGAC 
CACTTGTTGG AGGGGACCTT CAATCAGGTG ATGGCATACT CTCGGAAGGA GCTCCAGAGA 
AACAAGCTCT ACATCACCTT TGTTGGAGAG GAGGGGCTGG ACTACAGTGG CCCTTCTCGA 
GAGTTCTTCT TCCTGTTGTC TCAGGAGCTC TTCAACCCTT ACTATGGACT CTTTGAGTAT 
TCTGCAAATG ACACTTACAC AGTGCAGATC AGCCCCATGT CGGCGTTTGT AGAAAATTAT 
CTCGAATGGT TTAGATTCAG CGGCCGTATC CTGGGCCTAG CTCTGATCCA CCAGTACCTC 
TTGGATGCTT TCTTCACCAG ACCCTTCTAT AAGGGACTCC TGAAGCTGCC ATGCGATCTG 
AGTGACCTGG AATACTTGGA TGAGGAATTC CACCAGAGTT TACAGTGGAT GAAGGACAAC 
AACATCACAG ATATTCTTGA CCTCACTTTC ACTGTTAACG AAGAAGTTTT TGGACAGGTA 
ACAGAAAGAG AGTTGAAGTC TGGAGGTGCC AACACGCAGG TGACTGAGAA GAACAAGAAG 
GAGTACATCG AGAGGATGGT GAAGTGGCGT GTAGAGCGTG GTGTGGTACA GCAGACCGAG 
GCATTGGTCA GGGGTTTCTA TGAGGTTGTA GACTCAAGGC TTGTCTCAGT GTTTGACGCG 
AGGGAGCTGG AGCTGGTAAT CGCGGGAACT GCTGAAATCG ACCTGAATGA CTGGAGGAAT 
AACACTGAGT ACCGGGGAGG TTATCATGAT GGCCACCTTG TGATCCGCTG GTTCTGGGCT 
GCAGTGGAGC GCTTCAATAA TGAGCAGAGA TTGAGATTAC TTCAGTTTGT GACAGGAACG 
TCGAGTGTTC CCTATGAAGG CTTTGCAGCT CTGCGGGGGA GCAATGGGCT TCGAAGGTTC 
TGCATAGAGA AGTGGGGGAA GATTACATCT CTTCCCAGGG CACATACATG CTTCAACCGA 
CTGGATCTCC CACCGTATCC TTCATACTCC ACATTGTATG AAAAGTTGTT AACAGCAGTT 
GAAGAAACCA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_017600488.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455977.1 Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X5, mRNA.

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CloneID	ORa59017
Clone ID Related Accession (Same CDS sequence)	XM_017600488.1
Accession Version	XM_017600488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase HECW1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTC CATCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAAGAGCC CCTCCGGTAC 
AGCTATAACC CAGACCAATT CCACAACATA GACATCAGAA ATGGTGCCCA TGATGGCATC 
ACCATCCCTC GCTCTACCAG TGACACTGAC CTGGTCACCT CAGACAGCCG CTCTACACTC 
ATGGTCAGCA GTTCCTACTA TTCTATAGGG TATTCCCAGG ATTTGGTGAT CCACTGGGAC 
ATCAAGGAAG AAGTGGATGC CGGAGACTGG ATTGGGATGT ACCTTATTGG TGAAGTCTCA 
TCTGAAAACT TTCTGGACTA TAAGAACCGT GGAGTCAATG GATCTCACAG AGGCCAGATC 
ATTTGGAAGA TTGATGCCAG CTCTTACTTT GTGGAATCTG AAACTAAGAT CTGCTTCAAA 
TACTACCACG GAGTAAGCGG GGCCCTGAGA GCTACAACCC CCAGTGTCAC TGTCAAGAAC 
TCAGCGGCTC CTATTTTTAA AGGTATTGGC TCTGAGGAGA CTGTGCAAAG TCAAGGAAAC 
AGGAGGCTCA TCAGCTTCTC TCTCTCAGAT TTCCAGGCCA TGGGATTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA TCTGAAGATT TCCATTCAAC CTGGTAAACA TAGCATCTTC 
CCGGCCCTCC CTCATCATGG ACAGGAACGG AGATCCACGA TCATAGGCAA CACTGTAAAC 
CCCATCTGGC AGGCAGAGCA TTTCAGTTTC GTGTCTCTGC CAACTGATGT GCTGGAAATT 
GAAGTGAAGG ACAAGTTTGC CAAGAGTCGT CCCATCATCA AGCGTTTCTT GGGAAAGCTG 
TCAATGCCAG TTCAGAGGCT ACTGGAGAGA CATGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACTGACCAT GTGAGTGGAC ATCTGCAATT CCGATTTGAG 
ATCACATCCT CCATCCACGC AGACGATGAA GAGATCTCTC TGAATGCTGA GCCTGAGTCA 
GCGGAAGTGC AGGACAGTGT CATGAATAGT GTGGTGGAAA ACAGCAGTGG GGAGCCTCCC 
GGTGATGCTG CAGAATTCCT CAAGGACTTG AAGCCAGAGG CACCGAGTGA AGAGGATGGA 
GTGCGCGGCT CAGAAAATGA AAACCGAGAG CATGCCAGGC CAGTCGAGGA AGCTGCGGGT 
GCTATGGAGA CCAGAGATGA TAGCAAGTTC TCTGAAGGAC CGGAAGAGGT TGGAGAGCTT 
CAGGACCCAG AGCAACATGA CACCCAGCCC ACTCTGAGCG CAGAAGAAGT GGCAGAGCGA 
CTTCCCCTGG GTGAGGGTTG CCCACCCTCG CTGTTGCCAG AAGAGAGTAC AGCTCTAGGC 
AGCAAGGCAG AAGAGGAAGC AATTCCAGAA AATGGAGCGG GAGAGGAAGA GATGCAGGAG 
AAGGGGAAAG ATGAGGAGGA AGAAGAGGGT GTTTCTGCCC TGGAGCAAGG AGAGCCTGGA 
CTTGAGCTGA GGGCCTCCGT GAGAAAAAAA AGCAGGCCAT GTTCCTTACC TGTATCCGAG 
CTTGAAACAG TGATCGCATC TGCCTGTGGA GAGGCTGAGA CCCCAAGGAC CCACTACATC 
CGCATCCACA CCCTACTGCA CAGCATGCCC TCGGCCCAGA GGGGAAGCAC CACAGAGGAG 
GAGGATGCCA TGGAGGAGGA ATCCACCCTC AAGGAGTCCT CTGAAAAGGA TGGGCTCAGC 
GAGGTGGACA CAATAGCTGC TGACCCACAG TCCATGGAAG ATGGCGAGAA CAATGGCGCT 
ACTCTCTGCA TGGCATCCTC TGACTGCTCT GGGGGCCATT TCTCCAATTT ATCTAAAAGC 
ATTGGCGCTG GCCAAGATGG CGAAGCTCAC CCCAGCACTG GGAGTGAGAG TGACTCCAGC 
CCCCAGCAGG GAGCAGACCA CAGTTGCGAG GGCTGTGATG CCTCATGCTG TAGCCCCTCA 
TGCTATAGCA CATCGTGCTA CAGCAGCTCC TGTTATAGCA GCTCCTGTTA CAGCAGCTCC 
TGCTACAATG GCAACAATCG GTTTGCCAGC CACACACGCT TCTCCTCGGT GGACAGCGCC 
AAGATCTCTG AGAGCACAGT CTTTTCTTCA CAAGAGGATG AGGAGGAGGA AAACAGTGCA 
TTCGAGTCGG TACCTGACTC GGTGCAAAGC CCAGAGCTAG ATCCCGAGTC TACAAATGGG 
GCTGGGCCTT GGCAGGATGA ACTGGCTGCC CCTGGTGGGA ATGCAGCAAG AACTACTGAA 
GGTCTGGACT CCCCCATGGC AGGCCCTAGC AATAGGAGAG AAGGTGACTG TCCTATGCTC 
CATAATTCCC AGCCAATAAG CCAGCTTCCT TCCTTGAGGC CTGAACATCA TCACTACCCA 
ACAATCGATG AGCCTCTTCC ACCAAACTGG GAAGCTCGAA TCGACAGCCA TGGACGGGTC 
TTTTATGTGG ACCATGTAAA TCGCACAACC ACCTGGCAGC GACCATCAAT GGCGCCTACC 
CCAGATGGAA TGATCAGATC CGGGTCTGTC CACCAGATGG AGCAGCTTAA CAGAAGGTAT 
CAAAACATCC AGAGAACCAT TGCAACAGAG AGATCTGAAG AAGATTCTGG CAACCAAAGC 
TGTGAGCAAA TACCAGACGG AGGAGCTGGA GGTGGAGGGA GTGACTCGGA AGCAGAATCT 
TCCCAATCAA ACTTAGATCT GAGGAGAGAA GGGTCACTTT CTCCTGTCAA CTCACAGAAG 
GTCACCTTGC TGCTGCAGTC TCCAGCAGTC AAGTTCATCA CCAATCCTGA GTTCTTCACT 
GTGTTGCATG CCAATTATAG TGCCTACAGG GTTTTTACAA GTAGCACTTG CCTGAAGCAT 
ATGATTTTGA AAGTCCGAAG AGATGCTCGC AATTTTGAAC GCTACCAGCA CAACCGGGAC 
CTGGTGAATT TCATCAACAT GTTTGCAGAT ACTCGGCTGG AACTGCCCCG GGGTTGGGAG 
ATCAAAACTG ATCATCAGGG AAAGTCTTTC TTTGTGGACC ACAACAGTCG GGCTACTACT 
TTCATTGATC CCCGAATCCC TCTTCAGAAT GGCCGCCTTC CCAATCATCT AACCCACCGA 
CAGCACCTCC AGAGGCTCAG GAGTTACAGT GCTGGTGAGG CCTCAGAAGT CTCTAGAAAC 
AGAGGAGCAT CTTTGCTGGC CAGACCAGGA CACAGCCTAA TAGCTGCTAT TCGGAGCCAA 
CATCAACACG AGTCCTTGCC ACTGGCATAC AATGACAAGA TTGTGGCATT TCTTCGACAG 
CCAAACATTT TTGAAATGCT ACAAGAACGT CAGCCAAGCT TGGCAAGAAA CCACACACTC 
AGGGAGAAAA TCCATTATAT TCGGACAGAG GGCAATCATG GACTTGAGAA ATTGTCCTGT 
GATGCAGATC TAGTCATTTT GCTAAGTCTC TTTGAAGAAG AGATCATGTC CTATGTCCCC 
CTGCAATCTG CCTTTCACCC TGGATATAGC TTCTCTCCCC GCTGTTCACC CTGTTCCTCA 
CCCCAGAACT CCCCAGGTTT ACAGAGAGCC AGTGCCAGGG CCCCTTCACC GTACCGAAGG 
GATTTTGAAG CTAAGCTCCG CAATTTCTAC CGAAAACTGG AAGCCAAAGG ATTTGGGCAG 
GGTCCGGGTA AAATTAAGCT CATCATCCGT CGAGACCACT TGTTGGAGGG GACCTTCAAT 
CAGGTGATGG CATACTCTCG GAAGGAGCTC CAGAGAAACA AGCTCTACAT CACCTTTGTT 
GGAGAGGAGG GGCTGGACTA CAGTGGCCCT TCTCGAGAGT TCTTCTTCCT GTTGTCTCAG 
GAGCTCTTCA ACCCTTACTA TGGACTCTTT GAGTATTCTG CAAATGACAC TTACACAGTG 
CAGATCAGCC CCATGTCGGC GTTTGTAGAA AATTATCTCG AATGGTTTAG ATTCAGCGGC 
CGTATCCTGG GCCTAGCTCT GATCCACCAG TACCTCTTGG ATGCTTTCTT CACCAGACCC 
TTCTATAAGG GACTCCTGAA GCTGCCATGC GATCTGAGTG ACCTGGAATA CTTGGATGAG 
GAATTCCACC AGAGTTTACA GTGGATGAAG GACAACAACA TCACAGATAT TCTTGACCTC 
ACTTTCACTG TTAACGAAGA AGTTTTTGGA CAGGTAACAG AAAGAGAGTT GAAGTCTGGA 
GGTGCCAACA CGCAGGTGAC TGAGAAGAAC AAGAAGGAGT ACATCGAGAG GATGGTGAAG 
TGGCGTGTAG AGCGTGGTGT GGTACAGCAG ACCGAGGCAT TGGTCAGGGG TTTCTATGAG 
GTTGTAGACT CAAGGCTTGT CTCAGTGTTT GACGCGAGGG AGCTGGAGCT GGTAATCGCG 
GGAACTGCTG AAATCGACCT GAATGACTGG AGGAATAACA CTGAGTACCG GGGAGGTTAT 
CATGATGGCC ACCTTGTGAT CCGCTGGTTC TGGGCTGCAG TGGAGCGCTT CAATAATGAG 
CAGAGATTGA GATTACTTCA GTTTGTGACA GGAACGTCGA GTGTTCCCTA TGAAGGCTTT 
GCAGCTCTGC GGGGGAGCAA TGGGCTTCGA AGGTTCTGCA TAGAGAAGTG GGGGAAGATT 
ACATCTCTTC CCAGGGCACA TACATGCTTC AACCGACTGG ATCTCCCACC GTATCCTTCA 
TACTCCACAT TGTATGAAAA GTTGTTAACA GCAGTTGAAG AAACCAGCAC CTTTGGACTT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455977.1
CDS	100..4845
Translation	MASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDGITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGYSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETVQSQGNRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGHLQFRFEITSSIHADDEEISLNAEPESAEVQDSVMNSVVENSSGEPPGDAAEFLKDLKPEAPSEEDGVRGSENENREHARPVEEAAGAMETRDDSKFSEGPEEVGELQDPEQHDTQPTLSAEEVAERLPLGEGCPPSLLPEESTALGSKAEEEAIPENGAGEEEMQEKGKDEEEEEGVSALEQGEPGLELRASVRKKSRPCSLPVSELETVIASACGEAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDAMEEESTLKESSEKDGLSEVDTIAADPQSMEDGENNGATLCMASSDCSGGHFSNLSKSIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARTTEGLDSPMAGPSNRREGDCPMLHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTIATERSEEDSGNQSCEQIPDGGAGGGGSDSEAESSQSNLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSTLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017600488.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600488.1

ORF Insert Sequence:

ATGGCATCTC CATCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAAGAGCC CCTCCGGTAC 
AGCTATAACC CAGACCAATT CCACAACATA GACATCAGAA ATGGTGCCCA TGATGGCATC 
ACCATCCCTC GCTCTACCAG TGACACTGAC CTGGTCACCT CAGACAGCCG CTCTACACTC 
ATGGTCAGCA GTTCCTACTA TTCTATAGGG TATTCCCAGG ATTTGGTGAT CCACTGGGAC 
ATCAAGGAAG AAGTGGATGC CGGAGACTGG ATTGGGATGT ACCTTATTGG TGAAGTCTCA 
TCTGAAAACT TTCTGGACTA TAAGAACCGT GGAGTCAATG GATCTCACAG AGGCCAGATC 
ATTTGGAAGA TTGATGCCAG CTCTTACTTT GTGGAATCTG AAACTAAGAT CTGCTTCAAA 
TACTACCACG GAGTAAGCGG GGCCCTGAGA GCTACAACCC CCAGTGTCAC TGTCAAGAAC 
TCAGCGGCTC CTATTTTTAA AGGTATTGGC TCTGAGGAGA CTGTGCAAAG TCAAGGAAAC 
AGGAGGCTCA TCAGCTTCTC TCTCTCAGAT TTCCAGGCCA TGGGATTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA TCTGAAGATT TCCATTCAAC CTGGTAAACA TAGCATCTTC 
CCGGCCCTCC CTCATCATGG ACAGGAACGG AGATCCACGA TCATAGGCAA CACTGTAAAC 
CCCATCTGGC AGGCAGAGCA TTTCAGTTTC GTGTCTCTGC CAACTGATGT GCTGGAAATT 
GAAGTGAAGG ACAAGTTTGC CAAGAGTCGT CCCATCATCA AGCGTTTCTT GGGAAAGCTG 
TCAATGCCAG TTCAGAGGCT ACTGGAGAGA CATGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACTGACCAT GTGAGTGGAC ATCTGCAATT CCGATTTGAG 
ATCACATCCT CCATCCACGC AGACGATGAA GAGATCTCTC TGAATGCTGA GCCTGAGTCA 
GCGGAAGTGC AGGACAGTGT CATGAATAGT GTGGTGGAAA ACAGCAGTGG GGAGCCTCCC 
GGTGATGCTG CAGAATTCCT CAAGGACTTG AAGCCAGAGG CACCGAGTGA AGAGGATGGA 
GTGCGCGGCT CAGAAAATGA AAACCGAGAG CATGCCAGGC CAGTCGAGGA AGCTGCGGGT 
GCTATGGAGA CCAGAGATGA TAGCAAGTTC TCTGAAGGAC CGGAAGAGGT TGGAGAGCTT 
CAGGACCCAG AGCAACATGA CACCCAGCCC ACTCTGAGCG CAGAAGAAGT GGCAGAGCGA 
CTTCCCCTGG GTGAGGGTTG CCCACCCTCG CTGTTGCCAG AAGAGAGTAC AGCTCTAGGC 
AGCAAGGCAG AAGAGGAAGC AATTCCAGAA AATGGAGCGG GAGAGGAAGA GATGCAGGAG 
AAGGGGAAAG ATGAGGAGGA AGAAGAGGGT GTTTCTGCCC TGGAGCAAGG AGAGCCTGGA 
CTTGAGCTGA GGGCCTCCGT GAGAAAAAAA AGCAGGCCAT GTTCCTTACC TGTATCCGAG 
CTTGAAACAG TGATCGCATC TGCCTGTGGA GAGGCTGAGA CCCCAAGGAC CCACTACATC 
CGCATCCACA CCCTACTGCA CAGCATGCCC TCGGCCCAGA GGGGAAGCAC CACAGAGGAG 
GAGGATGCCA TGGAGGAGGA ATCCACCCTC AAGGAGTCCT CTGAAAAGGA TGGGCTCAGC 
GAGGTGGACA CAATAGCTGC TGACCCACAG TCCATGGAAG ATGGCGAGAA CAATGGCGCT 
ACTCTCTGCA TGGCATCCTC TGACTGCTCT GGGGGCCATT TCTCCAATTT ATCTAAAAGC 
ATTGGCGCTG GCCAAGATGG CGAAGCTCAC CCCAGCACTG GGAGTGAGAG TGACTCCAGC 
CCCCAGCAGG GAGCAGACCA CAGTTGCGAG GGCTGTGATG CCTCATGCTG TAGCCCCTCA 
TGCTATAGCA CATCGTGCTA CAGCAGCTCC TGTTATAGCA GCTCCTGTTA CAGCAGCTCC 
TGCTACAATG GCAACAATCG GTTTGCCAGC CACACACGCT TCTCCTCGGT GGACAGCGCC 
AAGATCTCTG AGAGCACAGT CTTTTCTTCA CAAGAGGATG AGGAGGAGGA AAACAGTGCA 
TTCGAGTCGG TACCTGACTC GGTGCAAAGC CCAGAGCTAG ATCCCGAGTC TACAAATGGG 
GCTGGGCCTT GGCAGGATGA ACTGGCTGCC CCTGGTGGGA ATGCAGCAAG AACTACTGAA 
GGTCTGGACT CCCCCATGGC AGGCCCTAGC AATAGGAGAG AAGGTGACTG TCCTATGCTC 
CATAATTCCC AGCCAATAAG CCAGCTTCCT TCCTTGAGGC CTGAACATCA TCACTACCCA 
ACAATCGATG AGCCTCTTCC ACCAAACTGG GAAGCTCGAA TCGACAGCCA TGGACGGGTC 
TTTTATGTGG ACCATGTAAA TCGCACAACC ACCTGGCAGC GACCATCAAT GGCGCCTACC 
CCAGATGGAA TGATCAGATC CGGGTCTGTC CACCAGATGG AGCAGCTTAA CAGAAGGTAT 
CAAAACATCC AGAGAACCAT TGCAACAGAG AGATCTGAAG AAGATTCTGG CAACCAAAGC 
TGTGAGCAAA TACCAGACGG AGGAGCTGGA GGTGGAGGGA GTGACTCGGA AGCAGAATCT 
TCCCAATCAA ACTTAGATCT GAGGAGAGAA GGGTCACTTT CTCCTGTCAA CTCACAGAAG 
GTCACCTTGC TGCTGCAGTC TCCAGCAGTC AAGTTCATCA CCAATCCTGA GTTCTTCACT 
GTGTTGCATG CCAATTATAG TGCCTACAGG GTTTTTACAA GTAGCACTTG CCTGAAGCAT 
ATGATTTTGA AAGTCCGAAG AGATGCTCGC AATTTTGAAC GCTACCAGCA CAACCGGGAC 
CTGGTGAATT TCATCAACAT GTTTGCAGAT ACTCGGCTGG AACTGCCCCG GGGTTGGGAG 
ATCAAAACTG ATCATCAGGG AAAGTCTTTC TTTGTGGACC ACAACAGTCG GGCTACTACT 
TTCATTGATC CCCGAATCCC TCTTCAGAAT GGCCGCCTTC CCAATCATCT AACCCACCGA 
CAGCACCTCC AGAGGCTCAG GAGTTACAGT GCTGGTGAGG CCTCAGAAGT CTCTAGAAAC 
AGAGGAGCAT CTTTGCTGGC CAGACCAGGA CACAGCCTAA TAGCTGCTAT TCGGAGCCAA 
CATCAACACG AGTCCTTGCC ACTGGCATAC AATGACAAGA TTGTGGCATT TCTTCGACAG 
CCAAACATTT TTGAAATGCT ACAAGAACGT CAGCCAAGCT TGGCAAGAAA CCACACACTC 
AGGGAGAAAA TCCATTATAT TCGGACAGAG GGCAATCATG GACTTGAGAA ATTGTCCTGT 
GATGCAGATC TAGTCATTTT GCTAAGTCTC TTTGAAGAAG AGATCATGTC CTATGTCCCC 
CTGCAATCTG CCTTTCACCC TGGATATAGC TTCTCTCCCC GCTGTTCACC CTGTTCCTCA 
CCCCAGAACT CCCCAGGTTT ACAGAGAGCC AGTGCCAGGG CCCCTTCACC GTACCGAAGG 
GATTTTGAAG CTAAGCTCCG CAATTTCTAC CGAAAACTGG AAGCCAAAGG ATTTGGGCAG 
GGTCCGGGTA AAATTAAGCT CATCATCCGT CGAGACCACT TGTTGGAGGG GACCTTCAAT 
CAGGTGATGG CATACTCTCG GAAGGAGCTC CAGAGAAACA AGCTCTACAT CACCTTTGTT 
GGAGAGGAGG GGCTGGACTA CAGTGGCCCT TCTCGAGAGT TCTTCTTCCT GTTGTCTCAG 
GAGCTCTTCA ACCCTTACTA TGGACTCTTT GAGTATTCTG CAAATGACAC TTACACAGTG 
CAGATCAGCC CCATGTCGGC GTTTGTAGAA AATTATCTCG AATGGTTTAG ATTCAGCGGC 
CGTATCCTGG GCCTAGCTCT GATCCACCAG TACCTCTTGG ATGCTTTCTT CACCAGACCC 
TTCTATAAGG GACTCCTGAA GCTGCCATGC GATCTGAGTG ACCTGGAATA CTTGGATGAG 
GAATTCCACC AGAGTTTACA GTGGATGAAG GACAACAACA TCACAGATAT TCTTGACCTC 
ACTTTCACTG TTAACGAAGA AGTTTTTGGA CAGGTAACAG AAAGAGAGTT GAAGTCTGGA 
GGTGCCAACA CGCAGGTGAC TGAGAAGAAC AAGAAGGAGT ACATCGAGAG GATGGTGAAG 
TGGCGTGTAG AGCGTGGTGT GGTACAGCAG ACCGAGGCAT TGGTCAGGGG TTTCTATGAG 
GTTGTAGACT CAAGGCTTGT CTCAGTGTTT GACGCGAGGG AGCTGGAGCT GGTAATCGCG 
GGAACTGCTG AAATCGACCT GAATGACTGG AGGAATAACA CTGAGTACCG GGGAGGTTAT 
CATGATGGCC ACCTTGTGAT CCGCTGGTTC TGGGCTGCAG TGGAGCGCTT CAATAATGAG 
CAGAGATTGA GATTACTTCA GTTTGTGACA GGAACGTCGA GTGTTCCCTA TGAAGGCTTT 
GCAGCTCTGC GGGGGAGCAA TGGGCTTCGA AGGTTCTGCA TAGAGAAGTG GGGGAAGATT 
ACATCTCTTC CCAGGGCACA TACATGCTTC AACCGACTGG ATCTCCCACC GTATCCTTCA 
TACTCCACAT TGTATGAAAA GTTGTTAACA GCAGTTGAAG AAACCAGCAC CTTTGGACTT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_017600489.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455978.1 Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa59018
Clone ID Related Accession (Same CDS sequence)	XM_017600489.1
Accession Version	XM_017600489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase HECW1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGCTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CATCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAGCCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GGCATCACCA TCCCTCGCTC TACCAGTGAC ACTGACCTGG TCACCTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCT ATAGGGTATT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCCGGA GACTGGATTG GGATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGATC TCACAGAGGC 
CAGATCATTT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGCGGGGCC CTGAGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CGGCTCCTAT TTTTAAAGGT ATTGGCTCTG AGGAGACTGT GCAAAGTCAA 
GGAAACAGGA GGCTCATCAG CTTCTCTCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAACCTGG TAAACATAGC 
ATCTTCCCGG CCCTCCCTCA TCATGGACAG GAACGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC TGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCAGTTCA GAGGCTACTG GAGAGACATG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACT GACCATGTGA GTGGACATCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCACGCAGAC GATGAAGAGA TCTCTCTGAA TGCTGAGCCT 
GAGTCAGCGG AAGTGCAGGA CAGTGTCATG AATAGTGTGG TGGAAAACAG CAGTGGGGAG 
CCTCCCGGTG ATGCTGCAGA ATTCCTCAAG GACTTGAAGC CAGAGGCACC GAGTGAAGAG 
GATGGAGTGC GCGGCTCAGA AAATGAAAAC CGAGAGCATG CCAGGCCAGT CGAGGAAGCT 
GCGGGTGCTA TGGAGACCAG AGATGATAGC AAGTTCTCTG AAGGACCGGA AGAGGTTGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACTC TGAGCGCAGA AGAAGTGGCA 
GAGCGACTTC CCCTGGGTGA GGGTTGCCCA CCCTCGCTGT TGCCAGAAGA GAGTACAGCT 
CTAGGCAGCA AGGCAGAAGA GGAAGCAATT CCAGAAAATG GAGCGGGAGA GGAAGAGATG 
CAGGAGAAGG GGAAAGATGA GGAGGAAGAA GAGGGTGTTT CTGCCCTGGA GCAAGGAGAG 
CCTGGACTTG AGCTGAGGGC CTCCGTGAGA AAAAAAAGCA GGCCATGTTC CTTACCTGTA 
TCCGAGCTTG AAACAGTGAT CGCATCTGCC TGTGGAGAGG CTGAGACCCC AAGGACCCAC 
TACATCCGCA TCCACACCCT ACTGCACAGC ATGCCCTCGG CCCAGAGGGG AAGCACCACA 
GAGGAGGAGG ATGCCATGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAACAAT 
GGCGCTACTC TCTGCATGGC ATCCTCTGAC TGCTCTGGGG GCCATTTCTC CAATTTATCT 
AAAAGCATTG GCGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGTAGC 
CCCTCATGCT ATAGCACATC GTGCTACAGC AGCTCCTGTT ATAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGCAA CAATCGGTTT GCCAGCCACA CACGCTTCTC CTCGGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA GGAGGAAAAC 
AGTGCATTCG AGTCGGTACC TGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GGCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAACT 
ACTGAAGGTC TGGACTCCCC CATGGCAGGC CCTAGCAATA GGAGAGAAGA CTGGGAAGCT 
CGAATCGACA GCCATGGACG GGTCTTTTAT GTGGACCATG TAAATCGCAC AACCACCTGG 
CAGCGACCAT CAATGGCGCC TACCCCAGAT GGAATGATCA GATCCGGGTC TGTCCACCAG 
ATGGAGCAGC TTAACAGAAG GTATCAAAAC ATCCAGAGAA CCATTGCAAC AGAGAGATCT 
GAAGAAGATT CTGGCAACCA AAGCTGTGAG CAAATACCAG ACGGAGGAGC TGGAGGTGGA 
GGGAGTGACT CGGAAGCAGA ATCTTCCCAA TCAAACTTAG ATCTGAGGAG AGAAGGGTCA 
CTTTCTCCTG TCAACTCACA GAAGGTCACC TTGCTGCTGC AGTCTCCAGC AGTCAAGTTC 
ATCACCAATC CTGAGTTCTT CACTGTGTTG CATGCCAATT ATAGTGCCTA CAGGGTTTTT 
ACAAGTAGCA CTTGCCTGAA GCATATGATT TTGAAAGTCC GAAGAGATGC TCGCAATTTT 
GAACGCTACC AGCACAACCG GGACCTGGTG AATTTCATCA ACATGTTTGC AGATACTCGG 
CTGGAACTGC CCCGGGGTTG GGAGATCAAA ACTGATCATC AGGGAAAGTC TTTCTTTGTG 
GACCACAACA GTCGGGCTAC TACTTTCATT GATCCCCGAA TCCCTCTTCA GAATGGCCGC 
CTTCCCAATC ATCTAACCCA CCGACAGCAC CTCCAGAGGC TCAGGAGTTA CAGTGCTGGT 
GAGGCCTCAG AAGTCTCTAG AAACAGAGGA GCATCTTTGC TGGCCAGACC AGGACACAGC 
CTAATAGCTG CTATTCGGAG CCAACATCAA CACGAGTCCT TGCCACTGGC ATACAATGAC 
AAGATTGTGG CATTTCTTCG ACAGCCAAAC ATTTTTGAAA TGCTACAAGA ACGTCAGCCA 
AGCTTGGCAA GAAACCACAC ACTCAGGGAG AAAATCCATT ATATTCGGAC AGAGGGCAAT 
CATGGACTTG AGAAATTGTC CTGTGATGCA GATCTAGTCA TTTTGCTAAG TCTCTTTGAA 
GAAGAGATCA TGTCCTATGT CCCCCTGCAA TCTGCCTTTC ACCCTGGATA TAGCTTCTCT 
CCCCGCTGTT CACCCTGTTC CTCACCCCAG AACTCCCCAG GTTTACAGAG AGCCAGTGCC 
AGGGCCCCTT CACCGTACCG AAGGGATTTT GAAGCTAAGC TCCGCAATTT CTACCGAAAA 
CTGGAAGCCA AAGGATTTGG GCAGGGTCCG GGTAAAATTA AGCTCATCAT CCGTCGAGAC 
CACTTGTTGG AGGGGACCTT CAATCAGGTG ATGGCATACT CTCGGAAGGA GCTCCAGAGA 
AACAAGCTCT ACATCACCTT TGTTGGAGAG GAGGGGCTGG ACTACAGTGG CCCTTCTCGA 
GAGTTCTTCT TCCTGTTGTC TCAGGAGCTC TTCAACCCTT ACTATGGACT CTTTGAGTAT 
TCTGCAAATG ACACTTACAC AGTGCAGATC AGCCCCATGT CGGCGTTTGT AGAAAATTAT 
CTCGAATGGT TTAGATTCAG CGGCCGTATC CTGGGCCTAG CTCTGATCCA CCAGTACCTC 
TTGGATGCTT TCTTCACCAG ACCCTTCTAT AAGGGACTCC TGAAGCTGCC ATGCGATCTG 
AGTGACCTGG AATACTTGGA TGAGGAATTC CACCAGAGTT TACAGTGGAT GAAGGACAAC 
AACATCACAG ATATTCTTGA CCTCACTTTC ACTGTTAACG AAGAAGTTTT TGGACAGGTA 
ACAGAAAGAG AGTTGAAGTC TGGAGGTGCC AACACGCAGG TGACTGAGAA GAACAAGAAG 
GAGTACATCG AGAGGATGGT GAAGTGGCGT GTAGAGCGTG GTGTGGTACA GCAGACCGAG 
GCATTGGTCA GGGGTTTCTA TGAGGTTGTA GACTCAAGGC TTGTCTCAGT GTTTGACGCG 
AGGGAGCTGG AGCTGGTAAT CGCGGGAACT GCTGAAATCG ACCTGAATGA CTGGAGGAAT 
AACACTGAGT ACCGGGGAGG TTATCATGAT GGCCACCTTG TGATCCGCTG GTTCTGGGCT 
GCAGTGGAGC GCTTCAATAA TGAGCAGAGA TTGAGATTAC TTCAGTTTGT GACAGGAACG 
TCGAGTGTTC CCTATGAAGG CTTTGCAGCT CTGCGGGGGA GCAATGGGCT TCGAAGGTTC 
TGCATAGAGA AGTGGGGGAA GATTACATCT CTTCCCAGGG CACATACATG CTTCAACCGA 
CTGGATCTCC CACCGTATCC TTCATACTCC ACATTGTATG AAAAGTTGTT AACAGCAGTT 
GAAGAAACCA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455978.1
CDS	37..4746
Translation	MICAQLEAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDGITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGYSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETVQSQGNRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGHLQFRFEITSSIHADDEEISLNAEPESAEVQDSVMNSVVENSSGEPPGDAAEFLKDLKPEAPSEEDGVRGSENENREHARPVEEAAGAMETRDDSKFSEGPEEVGELQDPEQHDTQPTLSAEEVAERLPLGEGCPPSLLPEESTALGSKAEEEAIPENGAGEEEMQEKGKDEEEEEGVSALEQGEPGLELRASVRKKSRPCSLPVSELETVIASACGEAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDAMEEESTLKESSEKDGLSEVDTIAADPQSMEDGENNGATLCMASSDCSGGHFSNLSKSIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARTTEGLDSPMAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTIATERSEEDSGNQSCEQIPDGGAGGGGSDSEAESSQSNLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSTLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017600489.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600489.1

ORF Insert Sequence:

ATGATCTGTG CCCAGCTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CATCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAGCCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GGCATCACCA TCCCTCGCTC TACCAGTGAC ACTGACCTGG TCACCTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCT ATAGGGTATT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCCGGA GACTGGATTG GGATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGATC TCACAGAGGC 
CAGATCATTT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGCGGGGCC CTGAGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CGGCTCCTAT TTTTAAAGGT ATTGGCTCTG AGGAGACTGT GCAAAGTCAA 
GGAAACAGGA GGCTCATCAG CTTCTCTCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAACCTGG TAAACATAGC 
ATCTTCCCGG CCCTCCCTCA TCATGGACAG GAACGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC TGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCAGTTCA GAGGCTACTG GAGAGACATG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACT GACCATGTGA GTGGACATCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCACGCAGAC GATGAAGAGA TCTCTCTGAA TGCTGAGCCT 
GAGTCAGCGG AAGTGCAGGA CAGTGTCATG AATAGTGTGG TGGAAAACAG CAGTGGGGAG 
CCTCCCGGTG ATGCTGCAGA ATTCCTCAAG GACTTGAAGC CAGAGGCACC GAGTGAAGAG 
GATGGAGTGC GCGGCTCAGA AAATGAAAAC CGAGAGCATG CCAGGCCAGT CGAGGAAGCT 
GCGGGTGCTA TGGAGACCAG AGATGATAGC AAGTTCTCTG AAGGACCGGA AGAGGTTGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACTC TGAGCGCAGA AGAAGTGGCA 
GAGCGACTTC CCCTGGGTGA GGGTTGCCCA CCCTCGCTGT TGCCAGAAGA GAGTACAGCT 
CTAGGCAGCA AGGCAGAAGA GGAAGCAATT CCAGAAAATG GAGCGGGAGA GGAAGAGATG 
CAGGAGAAGG GGAAAGATGA GGAGGAAGAA GAGGGTGTTT CTGCCCTGGA GCAAGGAGAG 
CCTGGACTTG AGCTGAGGGC CTCCGTGAGA AAAAAAAGCA GGCCATGTTC CTTACCTGTA 
TCCGAGCTTG AAACAGTGAT CGCATCTGCC TGTGGAGAGG CTGAGACCCC AAGGACCCAC 
TACATCCGCA TCCACACCCT ACTGCACAGC ATGCCCTCGG CCCAGAGGGG AAGCACCACA 
GAGGAGGAGG ATGCCATGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAACAAT 
GGCGCTACTC TCTGCATGGC ATCCTCTGAC TGCTCTGGGG GCCATTTCTC CAATTTATCT 
AAAAGCATTG GCGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGTAGC 
CCCTCATGCT ATAGCACATC GTGCTACAGC AGCTCCTGTT ATAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGCAA CAATCGGTTT GCCAGCCACA CACGCTTCTC CTCGGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA GGAGGAAAAC 
AGTGCATTCG AGTCGGTACC TGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GGCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAACT 
ACTGAAGGTC TGGACTCCCC CATGGCAGGC CCTAGCAATA GGAGAGAAGA CTGGGAAGCT 
CGAATCGACA GCCATGGACG GGTCTTTTAT GTGGACCATG TAAATCGCAC AACCACCTGG 
CAGCGACCAT CAATGGCGCC TACCCCAGAT GGAATGATCA GATCCGGGTC TGTCCACCAG 
ATGGAGCAGC TTAACAGAAG GTATCAAAAC ATCCAGAGAA CCATTGCAAC AGAGAGATCT 
GAAGAAGATT CTGGCAACCA AAGCTGTGAG CAAATACCAG ACGGAGGAGC TGGAGGTGGA 
GGGAGTGACT CGGAAGCAGA ATCTTCCCAA TCAAACTTAG ATCTGAGGAG AGAAGGGTCA 
CTTTCTCCTG TCAACTCACA GAAGGTCACC TTGCTGCTGC AGTCTCCAGC AGTCAAGTTC 
ATCACCAATC CTGAGTTCTT CACTGTGTTG CATGCCAATT ATAGTGCCTA CAGGGTTTTT 
ACAAGTAGCA CTTGCCTGAA GCATATGATT TTGAAAGTCC GAAGAGATGC TCGCAATTTT 
GAACGCTACC AGCACAACCG GGACCTGGTG AATTTCATCA ACATGTTTGC AGATACTCGG 
CTGGAACTGC CCCGGGGTTG GGAGATCAAA ACTGATCATC AGGGAAAGTC TTTCTTTGTG 
GACCACAACA GTCGGGCTAC TACTTTCATT GATCCCCGAA TCCCTCTTCA GAATGGCCGC 
CTTCCCAATC ATCTAACCCA CCGACAGCAC CTCCAGAGGC TCAGGAGTTA CAGTGCTGGT 
GAGGCCTCAG AAGTCTCTAG AAACAGAGGA GCATCTTTGC TGGCCAGACC AGGACACAGC 
CTAATAGCTG CTATTCGGAG CCAACATCAA CACGAGTCCT TGCCACTGGC ATACAATGAC 
AAGATTGTGG CATTTCTTCG ACAGCCAAAC ATTTTTGAAA TGCTACAAGA ACGTCAGCCA 
AGCTTGGCAA GAAACCACAC ACTCAGGGAG AAAATCCATT ATATTCGGAC AGAGGGCAAT 
CATGGACTTG AGAAATTGTC CTGTGATGCA GATCTAGTCA TTTTGCTAAG TCTCTTTGAA 
GAAGAGATCA TGTCCTATGT CCCCCTGCAA TCTGCCTTTC ACCCTGGATA TAGCTTCTCT 
CCCCGCTGTT CACCCTGTTC CTCACCCCAG AACTCCCCAG GTTTACAGAG AGCCAGTGCC 
AGGGCCCCTT CACCGTACCG AAGGGATTTT GAAGCTAAGC TCCGCAATTT CTACCGAAAA 
CTGGAAGCCA AAGGATTTGG GCAGGGTCCG GGTAAAATTA AGCTCATCAT CCGTCGAGAC 
CACTTGTTGG AGGGGACCTT CAATCAGGTG ATGGCATACT CTCGGAAGGA GCTCCAGAGA 
AACAAGCTCT ACATCACCTT TGTTGGAGAG GAGGGGCTGG ACTACAGTGG CCCTTCTCGA 
GAGTTCTTCT TCCTGTTGTC TCAGGAGCTC TTCAACCCTT ACTATGGACT CTTTGAGTAT 
TCTGCAAATG ACACTTACAC AGTGCAGATC AGCCCCATGT CGGCGTTTGT AGAAAATTAT 
CTCGAATGGT TTAGATTCAG CGGCCGTATC CTGGGCCTAG CTCTGATCCA CCAGTACCTC 
TTGGATGCTT TCTTCACCAG ACCCTTCTAT AAGGGACTCC TGAAGCTGCC ATGCGATCTG 
AGTGACCTGG AATACTTGGA TGAGGAATTC CACCAGAGTT TACAGTGGAT GAAGGACAAC 
AACATCACAG ATATTCTTGA CCTCACTTTC ACTGTTAACG AAGAAGTTTT TGGACAGGTA 
ACAGAAAGAG AGTTGAAGTC TGGAGGTGCC AACACGCAGG TGACTGAGAA GAACAAGAAG 
GAGTACATCG AGAGGATGGT GAAGTGGCGT GTAGAGCGTG GTGTGGTACA GCAGACCGAG 
GCATTGGTCA GGGGTTTCTA TGAGGTTGTA GACTCAAGGC TTGTCTCAGT GTTTGACGCG 
AGGGAGCTGG AGCTGGTAAT CGCGGGAACT GCTGAAATCG ACCTGAATGA CTGGAGGAAT 
AACACTGAGT ACCGGGGAGG TTATCATGAT GGCCACCTTG TGATCCGCTG GTTCTGGGCT 
GCAGTGGAGC GCTTCAATAA TGAGCAGAGA TTGAGATTAC TTCAGTTTGT GACAGGAACG 
TCGAGTGTTC CCTATGAAGG CTTTGCAGCT CTGCGGGGGA GCAATGGGCT TCGAAGGTTC 
TGCATAGAGA AGTGGGGGAA GATTACATCT CTTCCCAGGG CACATACATG CTTCAACCGA 
CTGGATCTCC CACCGTATCC TTCATACTCC ACATTGTATG AAAAGTTGTT AACAGCAGTT 
GAAGAAACCA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hecw1 ( XM_017600490.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455979.1 Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa59019
Clone ID Related Accession (Same CDS sequence)	XM_017600490.1
Accession Version	XM_017600490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase HECW1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGCTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CATCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAGCCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GGCATCACCA TCCCTCGCTC TACCAGTGAC ACTGACCTGG TCACCTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCT ATAGGGTATT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCCGGA GACTGGATTG GGATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGATC TCACAGAGGC 
CAGATCATTT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGCGGGGCC CTGAGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CGGCTCCTAT TTTTAAAGGT ATTGGCTCTG AGGAGACTGT GCAAAGTCAA 
GGAAACAGGA GGCTCATCAG CTTCTCTCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAACCTGG TAAACATAGC 
ATCTTCCCGG CCCTCCCTCA TCATGGACAG GAACGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC TGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCAGTTCA GAGGCTACTG GAGAGACATG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACT GACCATGTGA GTGGACATCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCACGCAGAC GATGAAGAGA TCTCTCTGAA TGCTGAGCCT 
GAGTCAGCGG AAGTGCAGGA CAGTGTCATG AATAGTGTGG TGGAAAACAG CAGTGGGGAG 
CCTCCCGGTG ATGCTGCAGA ATTCCTCAAG GACTTGAAGC CAGAGGCACC GAGTGAAGAG 
GATGGAGTGC GCGGCTCAGA AAATGAAAAC CGAGAGCATG CCAGGCCAGT CGAGGAAGCT 
GCGGGTGCTA TGGAGACCAG AGATGATAGC AAGTTCTCTG AAGGACCGGA AGAGGTTGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACTC TGAGCGCAGA AGAAGTGGCA 
GAGCGACTTC CCCTGGGTGA GGGTTGCCCA CCCTCGCTGT TGCCAGAAGA GAGTACAGCT 
CTAGGCAGCA AGGCAGAAGA GGAAGCAATT CCAGAAAATG GAGCGGGAGA GGAAGAGATG 
CAGGAGAAGG GGAAAGATGA GGAGGAAGAA GAGGGTGTTT CTGCCCTGGA GCAAGGAGAG 
CCTGGACTTG AGCTGAGGGC CTCCGTGAGA AAAAAAAGCA GGCCATGTTC CTTACCTGTA 
TCCGAGCTTG AAACAGTGAT CGCATCTGCC TGTGGAGAGG CTGAGACCCC AAGGACCCAC 
TACATCCGCA TCCACACCCT ACTGCACAGC ATGCCCTCGG CCCAGAGGGG AAGCACCACA 
GAGGAGGAGG ATGCCATGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAACAAT 
GGCGCTACTC TCTGCATGGC ATCCTCTGAC TGCTCTGGGG GCCATTTCTC CAATTTATCT 
AAAAGCATTG GCGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGTAGC 
CCCTCATGCT ATAGCACATC GTGCTACAGC AGCTCCTGTT ATAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGCAA CAATCGGTTT GCCAGCCACA CACGCTTCTC CTCGGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA GGAGGAAAAC 
AGTGCATTCG AGTCGGTACC TGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GGCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAACT 
ACTGAAGACT GGGAAGCTCG AATCGACAGC CATGGACGGG TCTTTTATGT GGACCATGTA 
AATCGCACAA CCACCTGGCA GCGACCATCA ATGGCGCCTA CCCCAGATGG AATGATCAGA 
TCCGGGTCTG TCCACCAGAT GGAGCAGCTT AACAGAAGGT ATCAAAACAT CCAGAGAACC 
ATTGCAACAG AGAGATCTGA AGAAGATTCT GGCAACCAAA GCTGTGAGCA AATACCAGAC 
GGAGGAGCTG GAGGTGGAGG GAGTGACTCG GAAGCAGAAT CTTCCCAATC AAACTTAGAT 
CTGAGGAGAG AAGGGTCACT TTCTCCTGTC AACTCACAGA AGGTCACCTT GCTGCTGCAG 
TCTCCAGCAG TCAAGTTCAT CACCAATCCT GAGTTCTTCA CTGTGTTGCA TGCCAATTAT 
AGTGCCTACA GGGTTTTTAC AAGTAGCACT TGCCTGAAGC ATATGATTTT GAAAGTCCGA 
AGAGATGCTC GCAATTTTGA ACGCTACCAG CACAACCGGG ACCTGGTGAA TTTCATCAAC 
ATGTTTGCAG ATACTCGGCT GGAACTGCCC CGGGGTTGGG AGATCAAAAC TGATCATCAG 
GGAAAGTCTT TCTTTGTGGA CCACAACAGT CGGGCTACTA CTTTCATTGA TCCCCGAATC 
CCTCTTCAGA ATGGCCGCCT TCCCAATCAT CTAACCCACC GACAGCACCT CCAGAGGCTC 
AGGAGTTACA GTGCTGGTGA GGCCTCAGAA GTCTCTAGAA ACAGAGGAGC ATCTTTGCTG 
GCCAGACCAG GACACAGCCT AATAGCTGCT ATTCGGAGCC AACATCAACA CGAGTCCTTG 
CCACTGGCAT ACAATGACAA GATTGTGGCA TTTCTTCGAC AGCCAAACAT TTTTGAAATG 
CTACAAGAAC GTCAGCCAAG CTTGGCAAGA AACCACACAC TCAGGGAGAA AATCCATTAT 
ATTCGGACAG AGGGCAATCA TGGACTTGAG AAATTGTCCT GTGATGCAGA TCTAGTCATT 
TTGCTAAGTC TCTTTGAAGA AGAGATCATG TCCTATGTCC CCCTGCAATC TGCCTTTCAC 
CCTGGATATA GCTTCTCTCC CCGCTGTTCA CCCTGTTCCT CACCCCAGAA CTCCCCAGGT 
TTACAGAGAG CCAGTGCCAG GGCCCCTTCA CCGTACCGAA GGGATTTTGA AGCTAAGCTC 
CGCAATTTCT ACCGAAAACT GGAAGCCAAA GGATTTGGGC AGGGTCCGGG TAAAATTAAG 
CTCATCATCC GTCGAGACCA CTTGTTGGAG GGGACCTTCA ATCAGGTGAT GGCATACTCT 
CGGAAGGAGC TCCAGAGAAA CAAGCTCTAC ATCACCTTTG TTGGAGAGGA GGGGCTGGAC 
TACAGTGGCC CTTCTCGAGA GTTCTTCTTC CTGTTGTCTC AGGAGCTCTT CAACCCTTAC 
TATGGACTCT TTGAGTATTC TGCAAATGAC ACTTACACAG TGCAGATCAG CCCCATGTCG 
GCGTTTGTAG AAAATTATCT CGAATGGTTT AGATTCAGCG GCCGTATCCT GGGCCTAGCT 
CTGATCCACC AGTACCTCTT GGATGCTTTC TTCACCAGAC CCTTCTATAA GGGACTCCTG 
AAGCTGCCAT GCGATCTGAG TGACCTGGAA TACTTGGATG AGGAATTCCA CCAGAGTTTA 
CAGTGGATGA AGGACAACAA CATCACAGAT ATTCTTGACC TCACTTTCAC TGTTAACGAA 
GAAGTTTTTG GACAGGTAAC AGAAAGAGAG TTGAAGTCTG GAGGTGCCAA CACGCAGGTG 
ACTGAGAAGA ACAAGAAGGA GTACATCGAG AGGATGGTGA AGTGGCGTGT AGAGCGTGGT 
GTGGTACAGC AGACCGAGGC ATTGGTCAGG GGTTTCTATG AGGTTGTAGA CTCAAGGCTT 
GTCTCAGTGT TTGACGCGAG GGAGCTGGAG CTGGTAATCG CGGGAACTGC TGAAATCGAC 
CTGAATGACT GGAGGAATAA CACTGAGTAC CGGGGAGGTT ATCATGATGG CCACCTTGTG 
ATCCGCTGGT TCTGGGCTGC AGTGGAGCGC TTCAATAATG AGCAGAGATT GAGATTACTT 
CAGTTTGTGA CAGGAACGTC GAGTGTTCCC TATGAAGGCT TTGCAGCTCT GCGGGGGAGC 
AATGGGCTTC GAAGGTTCTG CATAGAGAAG TGGGGGAAGA TTACATCTCT TCCCAGGGCA 
CATACATGCT TCAACCGACT GGATCTCCCA CCGTATCCTT CATACTCCAC ATTGTATGAA 
AAGTTGTTAA CAGCAGTTGA AGAAACCAGC ACCTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455979.1
CDS	37..4704
Translation	MICAQLEAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNIDIRNGAHDGITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGYSQDLVIHWDIKEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETVQSQGNRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGHLQFRFEITSSIHADDEEISLNAEPESAEVQDSVMNSVVENSSGEPPGDAAEFLKDLKPEAPSEEDGVRGSENENREHARPVEEAAGAMETRDDSKFSEGPEEVGELQDPEQHDTQPTLSAEEVAERLPLGEGCPPSLLPEESTALGSKAEEEAIPENGAGEEEMQEKGKDEEEEEGVSALEQGEPGLELRASVRKKSRPCSLPVSELETVIASACGEAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDAMEEESTLKESSEKDGLSEVDTIAADPQSMEDGENNGATLCMASSDCSGGHFSNLSKSIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARTTEDWEARIDSHGRVFYVDHVNRTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTIATERSEEDSGNQSCEQIPDGGAGGGGSDSEAESSQSNLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSTLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017600490.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (Hecw1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600490.1

ORF Insert Sequence:

ATGATCTGTG CCCAGCTGGA GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCTGCCATGG CATCTCCATC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA AGAGCCCCTC 
CGGTACAGCT ATAACCCAGA CCAATTCCAC AACATAGACA TCAGAAATGG TGCCCATGAT 
GGCATCACCA TCCCTCGCTC TACCAGTGAC ACTGACCTGG TCACCTCAGA CAGCCGCTCT 
ACACTCATGG TCAGCAGTTC CTACTATTCT ATAGGGTATT CCCAGGATTT GGTGATCCAC 
TGGGACATCA AGGAAGAAGT GGATGCCGGA GACTGGATTG GGATGTACCT TATTGGTGAA 
GTCTCATCTG AAAACTTTCT GGACTATAAG AACCGTGGAG TCAATGGATC TCACAGAGGC 
CAGATCATTT GGAAGATTGA TGCCAGCTCT TACTTTGTGG AATCTGAAAC TAAGATCTGC 
TTCAAATACT ACCACGGAGT AAGCGGGGCC CTGAGAGCTA CAACCCCCAG TGTCACTGTC 
AAGAACTCAG CGGCTCCTAT TTTTAAAGGT ATTGGCTCTG AGGAGACTGT GCAAAGTCAA 
GGAAACAGGA GGCTCATCAG CTTCTCTCTC TCAGATTTCC AGGCCATGGG ATTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAACCTGG TAAACATAGC 
ATCTTCCCGG CCCTCCCTCA TCATGGACAG GAACGGAGAT CCACGATCAT AGGCAACACT 
GTAAACCCCA TCTGGCAGGC AGAGCATTTC AGTTTCGTGT CTCTGCCAAC TGATGTGCTG 
GAAATTGAAG TGAAGGACAA GTTTGCCAAG AGTCGTCCCA TCATCAAGCG TTTCTTGGGA 
AAGCTGTCAA TGCCAGTTCA GAGGCTACTG GAGAGACATG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACT GACCATGTGA GTGGACATCT GCAATTCCGA 
TTTGAGATCA CATCCTCCAT CCACGCAGAC GATGAAGAGA TCTCTCTGAA TGCTGAGCCT 
GAGTCAGCGG AAGTGCAGGA CAGTGTCATG AATAGTGTGG TGGAAAACAG CAGTGGGGAG 
CCTCCCGGTG ATGCTGCAGA ATTCCTCAAG GACTTGAAGC CAGAGGCACC GAGTGAAGAG 
GATGGAGTGC GCGGCTCAGA AAATGAAAAC CGAGAGCATG CCAGGCCAGT CGAGGAAGCT 
GCGGGTGCTA TGGAGACCAG AGATGATAGC AAGTTCTCTG AAGGACCGGA AGAGGTTGGA 
GAGCTTCAGG ACCCAGAGCA ACATGACACC CAGCCCACTC TGAGCGCAGA AGAAGTGGCA 
GAGCGACTTC CCCTGGGTGA GGGTTGCCCA CCCTCGCTGT TGCCAGAAGA GAGTACAGCT 
CTAGGCAGCA AGGCAGAAGA GGAAGCAATT CCAGAAAATG GAGCGGGAGA GGAAGAGATG 
CAGGAGAAGG GGAAAGATGA GGAGGAAGAA GAGGGTGTTT CTGCCCTGGA GCAAGGAGAG 
CCTGGACTTG AGCTGAGGGC CTCCGTGAGA AAAAAAAGCA GGCCATGTTC CTTACCTGTA 
TCCGAGCTTG AAACAGTGAT CGCATCTGCC TGTGGAGAGG CTGAGACCCC AAGGACCCAC 
TACATCCGCA TCCACACCCT ACTGCACAGC ATGCCCTCGG CCCAGAGGGG AAGCACCACA 
GAGGAGGAGG ATGCCATGGA GGAGGAATCC ACCCTCAAGG AGTCCTCTGA AAAGGATGGG 
CTCAGCGAGG TGGACACAAT AGCTGCTGAC CCACAGTCCA TGGAAGATGG CGAGAACAAT 
GGCGCTACTC TCTGCATGGC ATCCTCTGAC TGCTCTGGGG GCCATTTCTC CAATTTATCT 
AAAAGCATTG GCGCTGGCCA AGATGGCGAA GCTCACCCCA GCACTGGGAG TGAGAGTGAC 
TCCAGCCCCC AGCAGGGAGC AGACCACAGT TGCGAGGGCT GTGATGCCTC ATGCTGTAGC 
CCCTCATGCT ATAGCACATC GTGCTACAGC AGCTCCTGTT ATAGCAGCTC CTGTTACAGC 
AGCTCCTGCT ACAATGGCAA CAATCGGTTT GCCAGCCACA CACGCTTCTC CTCGGTGGAC 
AGCGCCAAGA TCTCTGAGAG CACAGTCTTT TCTTCACAAG AGGATGAGGA GGAGGAAAAC 
AGTGCATTCG AGTCGGTACC TGACTCGGTG CAAAGCCCAG AGCTAGATCC CGAGTCTACA 
AATGGGGCTG GGCCTTGGCA GGATGAACTG GCTGCCCCTG GTGGGAATGC AGCAAGAACT 
ACTGAAGACT GGGAAGCTCG AATCGACAGC CATGGACGGG TCTTTTATGT GGACCATGTA 
AATCGCACAA CCACCTGGCA GCGACCATCA ATGGCGCCTA CCCCAGATGG AATGATCAGA 
TCCGGGTCTG TCCACCAGAT GGAGCAGCTT AACAGAAGGT ATCAAAACAT CCAGAGAACC 
ATTGCAACAG AGAGATCTGA AGAAGATTCT GGCAACCAAA GCTGTGAGCA AATACCAGAC 
GGAGGAGCTG GAGGTGGAGG GAGTGACTCG GAAGCAGAAT CTTCCCAATC AAACTTAGAT 
CTGAGGAGAG AAGGGTCACT TTCTCCTGTC AACTCACAGA AGGTCACCTT GCTGCTGCAG 
TCTCCAGCAG TCAAGTTCAT CACCAATCCT GAGTTCTTCA CTGTGTTGCA TGCCAATTAT 
AGTGCCTACA GGGTTTTTAC AAGTAGCACT TGCCTGAAGC ATATGATTTT GAAAGTCCGA 
AGAGATGCTC GCAATTTTGA ACGCTACCAG CACAACCGGG ACCTGGTGAA TTTCATCAAC 
ATGTTTGCAG ATACTCGGCT GGAACTGCCC CGGGGTTGGG AGATCAAAAC TGATCATCAG 
GGAAAGTCTT TCTTTGTGGA CCACAACAGT CGGGCTACTA CTTTCATTGA TCCCCGAATC 
CCTCTTCAGA ATGGCCGCCT TCCCAATCAT CTAACCCACC GACAGCACCT CCAGAGGCTC 
AGGAGTTACA GTGCTGGTGA GGCCTCAGAA GTCTCTAGAA ACAGAGGAGC ATCTTTGCTG 
GCCAGACCAG GACACAGCCT AATAGCTGCT ATTCGGAGCC AACATCAACA CGAGTCCTTG 
CCACTGGCAT ACAATGACAA GATTGTGGCA TTTCTTCGAC AGCCAAACAT TTTTGAAATG 
CTACAAGAAC GTCAGCCAAG CTTGGCAAGA AACCACACAC TCAGGGAGAA AATCCATTAT 
ATTCGGACAG AGGGCAATCA TGGACTTGAG AAATTGTCCT GTGATGCAGA TCTAGTCATT 
TTGCTAAGTC TCTTTGAAGA AGAGATCATG TCCTATGTCC CCCTGCAATC TGCCTTTCAC 
CCTGGATATA GCTTCTCTCC CCGCTGTTCA CCCTGTTCCT CACCCCAGAA CTCCCCAGGT 
TTACAGAGAG CCAGTGCCAG GGCCCCTTCA CCGTACCGAA GGGATTTTGA AGCTAAGCTC 
CGCAATTTCT ACCGAAAACT GGAAGCCAAA GGATTTGGGC AGGGTCCGGG TAAAATTAAG 
CTCATCATCC GTCGAGACCA CTTGTTGGAG GGGACCTTCA ATCAGGTGAT GGCATACTCT 
CGGAAGGAGC TCCAGAGAAA CAAGCTCTAC ATCACCTTTG TTGGAGAGGA GGGGCTGGAC 
TACAGTGGCC CTTCTCGAGA GTTCTTCTTC CTGTTGTCTC AGGAGCTCTT CAACCCTTAC 
TATGGACTCT TTGAGTATTC TGCAAATGAC ACTTACACAG TGCAGATCAG CCCCATGTCG 
GCGTTTGTAG AAAATTATCT CGAATGGTTT AGATTCAGCG GCCGTATCCT GGGCCTAGCT 
CTGATCCACC AGTACCTCTT GGATGCTTTC TTCACCAGAC CCTTCTATAA GGGACTCCTG 
AAGCTGCCAT GCGATCTGAG TGACCTGGAA TACTTGGATG AGGAATTCCA CCAGAGTTTA 
CAGTGGATGA AGGACAACAA CATCACAGAT ATTCTTGACC TCACTTTCAC TGTTAACGAA 
GAAGTTTTTG GACAGGTAAC AGAAAGAGAG TTGAAGTCTG GAGGTGCCAA CACGCAGGTG 
ACTGAGAAGA ACAAGAAGGA GTACATCGAG AGGATGGTGA AGTGGCGTGT AGAGCGTGGT 
GTGGTACAGC AGACCGAGGC ATTGGTCAGG GGTTTCTATG AGGTTGTAGA CTCAAGGCTT 
GTCTCAGTGT TTGACGCGAG GGAGCTGGAG CTGGTAATCG CGGGAACTGC TGAAATCGAC 
CTGAATGACT GGAGGAATAA CACTGAGTAC CGGGGAGGTT ATCATGATGG CCACCTTGTG 
ATCCGCTGGT TCTGGGCTGC AGTGGAGCGC TTCAATAATG AGCAGAGATT GAGATTACTT 
CAGTTTGTGA CAGGAACGTC GAGTGTTCCC TATGAAGGCT TTGCAGCTCT GCGGGGGAGC 
AATGGGCTTC GAAGGTTCTG CATAGAGAAG TGGGGGAAGA TTACATCTCT TCCCAGGGCA 
CATACATGCT TCAACCGACT GGATCTCCCA CCGTATCCTT CATACTCCAC ATTGTATGAA 
AAGTTGTTAA CAGCAGTTGA AGAAACCAGC ACCTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Briefings in bioinformatics12(5)449-62(2011 Sep)
Gaudet P,Livstone MS,Lewis SE,Thomas PD

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Hecw1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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