HECW1 cDNA ORF clone, Gallus gallus(chicken)

The following HECW1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa146111 XM_025147586.1
Latest version!
Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa146112 XM_025147587.1
Latest version!
Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa146113 XM_025147588.1
Latest version!
Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa31690 XM_015281615.2
Latest version!
Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa31691 XM_015281616.2
Latest version!
Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa31692 XM_015281617.2
Latest version!
Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa31693 XM_015281620.2
Latest version!
Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa31693 XM_015281619.2
Latest version!
Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa31694 XM_418870.6
Latest version!
Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa31695 XM_015281621.2
Latest version!
Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
View Old Accession Versions >>
OGa31692 XM_015281617.1 Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa31693 XM_015281619.1 Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa31693 XM_015281620.1 Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa31694 XM_418870.5 Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa31691 XM_015281616.1 Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa31695 XM_015281621.1 Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OGa31690 XM_015281615.1 Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa146111
    Clone ID Related Accession (Same CDS sequence) XM_025147586.1
    Accession Version XM_025147586.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
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    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGGGGATG TACTGGAAGA AATACAGCTA CCGGTTATGA AAGTCCCTCA GAATCTGTAC 
    CAAAACAGGT TCCTGGGCCT CGCAGCCATG GCGTCTCCAT CTAGGAACTC ACAAAATCGG
    CGCCGGTGCA AAGAGCCACT CAGATACAGC TATAACCCTG ACCAGTTTCA TAACATGGAC
    ATCAGGAACA ATTCCCATGA GACAGTCACC ATTCCTAGGT CTACCAGTGA CACGGACCTT
    GTCACTTCAG ACAGCCGATC TACCCTGATG GTCAGCAGCT CCTACTATTC CATTGGGCAC
    TCACAGGACC TAGTGATATA CTGGGATATA AAGGAGGAAG TGGATGCAGG GGACTGGATT
    GGCATGTATC TCATAGATGA AGTCTTGTCT GAAAACTTTT TGGACTACAA GAACCGTGGA
    GTCAATGGCT CTCATCGTGG TCAGATTGTC TGGAAGATTG ATGCCAGTTC TTACTTTGTG
    GAGCCTGAAA CAAAGATATG CTTCAAATAC TATCACGGTG TGAGTGGGGC ACTACGAGCC
    ACAACTCCAA GCGTCACGGT GAAAAACAAT GCAGCTCCTC AGATTTTTAA AAGCATCACC
    AATGAAGATT CAATCCAGGG ACAGGGAAAT CGGAGGCTGA TAAGTTTTTC ACTGTCAGAT
    TTCCAAGCTT TTGGTCTGAA GAAAGGAATG TTTTTCAATC CAGATCCTTA TCTGAAGATT
    TCCATCCAGC CTGGGAAACA CAGCATCTTC CCAGCACTTC CTCATCATGG ACAGGAGAAA
    AGGTCTAAGA TCATGTGTAA TACAGTTAAC CCAATCTGGC AAGGAGAGCA ATTCAGCTTT
    GTCTCACTGC CCACGGATGT CCTGGAAATA GAAGTTAAAG ACAAATTTGC AAAGAGCCGA
    CCAATCATCA AGCGTTTCCT GGGAAAACTC TCTATGCCAG TTCAGCGTCT CTTGGAAAGA
    CATGCCATAG GGGACAGGGT TGTAAGCTAC ACTCTTGGTC GCAGGCTTCC TACAGATCAT
    GTCAGTGGCC AGCTGCAGTT CCGATTTGAG ATAACTTCTT CCATTCATCA AGATGATGAA
    GAGGTTACCA TTAGTGCAGA TCCTGAGCCA GCTGACCTCC AGGAAAGCCC TGTGAACAAC
    CCCACAGAGG AAAGCCTCGG TGAGCCTCCA TGCATCAACA CAGTGACCTC AGAAAATACA
    GTTCCTGAAG AGCTAAATGG ATACAGTGTG AACAATATTG ATAGCCCAGG GGCTGTCAAA
    CCACCCAGGG AAGTCAACCA GAACAACATA AATGAAGAGC TAGCAGGAGA TGAGGAGGTT
    GATGCCATGC CAGTTCCTGA ACCCTTGGAA TCCATCCGAG GAGAAGTGCT GCCTTCTTTG
    AATGCGACAG ACCTCACAGA GCAGCTGGGT CTGCTGGCTT GCTTGTCTCC TTCTGAGACT
    GAAGTTAGGG AAAATAGCAA AGTCAATTCT GGTACTCAGG GAGATGATGG AGTATTGAAC
    AGTACAGAAA CTTTAGATGT TAGTGAGGTG AGTGCAGATG TATATGCTGA CAAAGACCTA
    GAGAAGAGTC ATGAAGAAGA AGGGGCTTCA GCCCGAGGGG AGGAAGACAG CAAGCTACAG
    CTGAGGACCA CAGCAAAGAG GAAAACAAGG CCATGCTCCC TGCCTGTGTC TGAAATTGAG
    ACCGTTATAG CTTCAGCTTG TGGTGAGCCA GAGACCCCTC GCACTCACTA TATACGAATC
    CACAACCTGC TCCACAGCCT GCCCTCAGCA CAGATGAGCA GTGATGGGGA AGAAGAGCAA
    GATGCTGGCA ATGGTGGGGA GAATGATGAA GAGTCTACAG TCAAGGAAGT GTTGCAGAAG
    GAGACAGTCA GTGAAAGTGA GACAATGGCA GCAGAGCCTC CTCCAGAAAA TGAGGCCGAA
    GAGAACTTCA GCCAGTGCAC AGTGCCTCCT GGGACCTTAG ACGAGCAACT CTCTGACTTA
    AATGATGGAC TGGTGGATGG GGAAAACCCT AGGACAGGAA GCGAAAGCGA GTCAAGCTCC
    AGACCCAATG GTGACAACGA ATGTGATTGT CCTTTACTCC ATAATTCTCA GCCAGTCAGC
    CAACTTCCTT CTCTGAGGCC TGACCATCAT CACTACCCAA CTATCGATGA GCCTCTTCCA
    CCAAATTGGG AAGCTCGGAT AGACAGCCAC GGGAGGGTGT TCTACGTTGA CCATGTCAAC
    CGAACTACCA CATGGCAGCG CCCCACAGCT GCAGCAACAC CAGATGGGAT CCACAGGTCT
    GGCTCTATCC AGCAAATGGA GCAGCTGAAC CGGCGATATC AAAATATCCA GCGAACTATT
    GCAACAGAGC GGACTGAAGA TGATGGTGTA GTAAACAACA GAGTGGAGAG AATTCCCACT
    GGAGGAGGAA GTGACTCTGA GGCAGAGCCT TCCCAACCAA GCTCCGAAAT TAGGAGGGAA
    GGTTCCCTTT CTCCTGTGAA TTCTCAAAAA ATCACCTTAT TGCTGCAGTC TCCAGCTGTG
    AAGTTTATCA CCAATCCTGA GTTCTTCACT GTGCTGCATG CAAACTATAG TGCCTATCGA
    GTCTTCACTA ACAGTACCTG CTTGAAGCAT ATGATCCTGA AGATCCGTAG AGATGCTCGT
    AATTTTGAGC GCTATCAGCA CAACAGAGAC CTTGTAAATT TTATCAACAT GTTTGCTGAC
    ACTCGACTAG AGCTTCCTCG TGGCTGGGAA ATAAAAACTG ACCAGCAGGG CAAGTCTTTC
    TTCGTTGACC ACAACAGTCG TGCTACCACA TTCATAGATC CCAGGATCCC ACTGCAGAAT
    GGTCGTCTCC CCAATCACTT GACTCACAGG CAACATCTTC AGCGGCTTCG TAGCTACAGT
    GCTGGAGAGG CTTCTGAAGT CTCTCGGAAC AGAGGAGTTA CTCTGTTGGC CAGACCAGGC
    AATAGTCTTG TAACAGCTAT TCGAAACCAG CATCATTATG AGACATTACC TCTGGCTTAC
    AATGACAAGA TTGTTGCATT TCTACGCCAA CCTAACATTT TTGAGATGCT GCAAGAGCGT
    CAGCCCAGTT TGGCCAGAAA TCATGCACTC AGGGAGAAGA TCCATTATAT TCGGACAGAG
    GGTACACATG GGCTTGAAAA GTTGTCCTGT GATGCAGATT TAGTAATTCT GCTGAGCCTT
    TTTGAAGAAG ATATCATGTC CTACATACCA CTCCAGGCCA CTTTCCATCC TGGTTACAGT
    TTCTCTCCTC GCTGCTCACC CTGCTCATCT CCCCAAAATT CTCCAGGACT GCAGCGAGCA
    AGTGCAAGAG CACCCTCTCC ATACAGGAGA GATTTTGAAG CCAAACTACG CAATTTCTAT
    CGAAAACTTG AAGCCAAAGG ATATGGACAA GGTCCAGGTA AAATTAAGTT AATTATACGA
    CGTGACCACT TACTGGAAGG CACCTTCAAC CAGGTGATGG CATATTCACG CAAGGAGCTC
    CAGCGAAACA AACTCTACAT CACATTTGTT GGCGAGGAAG GGCTGGACTA CAGTGGCCCC
    TCTCGAGAAT TCTTTTTCCT TCTGTCTCAG GAGCTCTTCA ATCCATATTA CGGGCTGTTT
    GAGTATTCAG CCAATGACAC TTACACTGTA CAGATTAGCC CCATGTCTGC CTTTGTTGAA
    AATCACCTGG AATGGTTCAG GTTCAGTGGT CGTATTCTTG GCCTTGCTCT CATTCATCAG
    TACCTTCTTG ATGCTTTCTT CACAAGGCCG TTCTACAAAG CACTATTAAG GCTGCCATGT
    GATCTAAGTG ACTTAGAGTA CCTGGATGAA GAATTCCATC AGAGCTTGCA GTGGATGAAA
    GATAACAACA TCACAGATAT CCTGGATCTC ACCTTCACAG TGAATGAGGA GGTGTTTGGA
    CAGGTGACAG AGAGAGAGTT GAAATCTGGA GGGGCAAATA CAGCAGTGAC AGAAAAGAAC
    AAAAAAGAGT ACATTGAGAG AATGGTTAAA TGGCGAGTGG AACGAGGAGT TGTTCAACAA
    ACAGAGGCCC TGGTCCGTGG CTTCTATGAA GTTGTAGACT CAAGGCTTGT CTCTGTTTTT
    GATGCCAGAG AACTGGAGCT GGTTATAGCT GGCACTGCAG AAATAGATCT CAATGACTGG
    CGTAACAACA CGGAATATCG TGGAGGTTAC CACGATGGAC ACATTGTAAT ACGGTGGTTC
    TGGGCAGCAG TGGAGAGGTT TAACAATGAA CAGAGACTCC GGTTGTTGCA GTTTGTTACT
    GGAACATCAA GTGTGCCATA TGAGGGCTTT GCAGCTCTCC GAGGAAGCAA TGGGCTTCGG
    CGTTTCTGTA TAGAGAAATG GGGGAAAATA ACCTCCCTTC CGAGGGCACA TACATGTTTC
    AACCGCTTGG ATCTTCCACC TTACCCCTCA TATTCTATGC TGTATGAAAA GCTGTTGACA
    GCTGTTGAAG AAACTAGTAC CTTTGGACTT GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025003354.1
    CDS166..4641
    Translation

    Target ORF information:

    RefSeq Version XM_025147586.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025147586.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGGGGATG TACTGGAAGA AATACAGCTA CCGGTTATGA AAGTCCCTCA GAATCTGTAC 
    CAAAACAGGT TCCTGGGCCT CGCAGCCATG GCGTCTCCAT CTAGGAACTC ACAAAATCGG
    CGCCGGTGCA AAGAGCCACT CAGATACAGC TATAACCCTG ACCAGTTTCA TAACATGGAC
    ATCAGGAACA ATTCCCATGA GACAGTCACC ATTCCTAGGT CTACCAGTGA CACGGACCTT
    GTCACTTCAG ACAGCCGATC TACCCTGATG GTCAGCAGCT CCTACTATTC CATTGGGCAC
    TCACAGGACC TAGTGATATA CTGGGATATA AAGGAGGAAG TGGATGCAGG GGACTGGATT
    GGCATGTATC TCATAGATGA AGTCTTGTCT GAAAACTTTT TGGACTACAA GAACCGTGGA
    GTCAATGGCT CTCATCGTGG TCAGATTGTC TGGAAGATTG ATGCCAGTTC TTACTTTGTG
    GAGCCTGAAA CAAAGATATG CTTCAAATAC TATCACGGTG TGAGTGGGGC ACTACGAGCC
    ACAACTCCAA GCGTCACGGT GAAAAACAAT GCAGCTCCTC AGATTTTTAA AAGCATCACC
    AATGAAGATT CAATCCAGGG ACAGGGAAAT CGGAGGCTGA TAAGTTTTTC ACTGTCAGAT
    TTCCAAGCTT TTGGTCTGAA GAAAGGAATG TTTTTCAATC CAGATCCTTA TCTGAAGATT
    TCCATCCAGC CTGGGAAACA CAGCATCTTC CCAGCACTTC CTCATCATGG ACAGGAGAAA
    AGGTCTAAGA TCATGTGTAA TACAGTTAAC CCAATCTGGC AAGGAGAGCA ATTCAGCTTT
    GTCTCACTGC CCACGGATGT CCTGGAAATA GAAGTTAAAG ACAAATTTGC AAAGAGCCGA
    CCAATCATCA AGCGTTTCCT GGGAAAACTC TCTATGCCAG TTCAGCGTCT CTTGGAAAGA
    CATGCCATAG GGGACAGGGT TGTAAGCTAC ACTCTTGGTC GCAGGCTTCC TACAGATCAT
    GTCAGTGGCC AGCTGCAGTT CCGATTTGAG ATAACTTCTT CCATTCATCA AGATGATGAA
    GAGGTTACCA TTAGTGCAGA TCCTGAGCCA GCTGACCTCC AGGAAAGCCC TGTGAACAAC
    CCCACAGAGG AAAGCCTCGG TGAGCCTCCA TGCATCAACA CAGTGACCTC AGAAAATACA
    GTTCCTGAAG AGCTAAATGG ATACAGTGTG AACAATATTG ATAGCCCAGG GGCTGTCAAA
    CCACCCAGGG AAGTCAACCA GAACAACATA AATGAAGAGC TAGCAGGAGA TGAGGAGGTT
    GATGCCATGC CAGTTCCTGA ACCCTTGGAA TCCATCCGAG GAGAAGTGCT GCCTTCTTTG
    AATGCGACAG ACCTCACAGA GCAGCTGGGT CTGCTGGCTT GCTTGTCTCC TTCTGAGACT
    GAAGTTAGGG AAAATAGCAA AGTCAATTCT GGTACTCAGG GAGATGATGG AGTATTGAAC
    AGTACAGAAA CTTTAGATGT TAGTGAGGTG AGTGCAGATG TATATGCTGA CAAAGACCTA
    GAGAAGAGTC ATGAAGAAGA AGGGGCTTCA GCCCGAGGGG AGGAAGACAG CAAGCTACAG
    CTGAGGACCA CAGCAAAGAG GAAAACAAGG CCATGCTCCC TGCCTGTGTC TGAAATTGAG
    ACCGTTATAG CTTCAGCTTG TGGTGAGCCA GAGACCCCTC GCACTCACTA TATACGAATC
    CACAACCTGC TCCACAGCCT GCCCTCAGCA CAGATGAGCA GTGATGGGGA AGAAGAGCAA
    GATGCTGGCA ATGGTGGGGA GAATGATGAA GAGTCTACAG TCAAGGAAGT GTTGCAGAAG
    GAGACAGTCA GTGAAAGTGA GACAATGGCA GCAGAGCCTC CTCCAGAAAA TGAGGCCGAA
    GAGAACTTCA GCCAGTGCAC AGTGCCTCCT GGGACCTTAG ACGAGCAACT CTCTGACTTA
    AATGATGGAC TGGTGGATGG GGAAAACCCT AGGACAGGAA GCGAAAGCGA GTCAAGCTCC
    AGACCCAATG GTGACAACGA ATGTGATTGT CCTTTACTCC ATAATTCTCA GCCAGTCAGC
    CAACTTCCTT CTCTGAGGCC TGACCATCAT CACTACCCAA CTATCGATGA GCCTCTTCCA
    CCAAATTGGG AAGCTCGGAT AGACAGCCAC GGGAGGGTGT TCTACGTTGA CCATGTCAAC
    CGAACTACCA CATGGCAGCG CCCCACAGCT GCAGCAACAC CAGATGGGAT CCACAGGTCT
    GGCTCTATCC AGCAAATGGA GCAGCTGAAC CGGCGATATC AAAATATCCA GCGAACTATT
    GCAACAGAGC GGACTGAAGA TGATGGTGTA GTAAACAACA GAGTGGAGAG AATTCCCACT
    GGAGGAGGAA GTGACTCTGA GGCAGAGCCT TCCCAACCAA GCTCCGAAAT TAGGAGGGAA
    GGTTCCCTTT CTCCTGTGAA TTCTCAAAAA ATCACCTTAT TGCTGCAGTC TCCAGCTGTG
    AAGTTTATCA CCAATCCTGA GTTCTTCACT GTGCTGCATG CAAACTATAG TGCCTATCGA
    GTCTTCACTA ACAGTACCTG CTTGAAGCAT ATGATCCTGA AGATCCGTAG AGATGCTCGT
    AATTTTGAGC GCTATCAGCA CAACAGAGAC CTTGTAAATT TTATCAACAT GTTTGCTGAC
    ACTCGACTAG AGCTTCCTCG TGGCTGGGAA ATAAAAACTG ACCAGCAGGG CAAGTCTTTC
    TTCGTTGACC ACAACAGTCG TGCTACCACA TTCATAGATC CCAGGATCCC ACTGCAGAAT
    GGTCGTCTCC CCAATCACTT GACTCACAGG CAACATCTTC AGCGGCTTCG TAGCTACAGT
    GCTGGAGAGG CTTCTGAAGT CTCTCGGAAC AGAGGAGTTA CTCTGTTGGC CAGACCAGGC
    AATAGTCTTG TAACAGCTAT TCGAAACCAG CATCATTATG AGACATTACC TCTGGCTTAC
    AATGACAAGA TTGTTGCATT TCTACGCCAA CCTAACATTT TTGAGATGCT GCAAGAGCGT
    CAGCCCAGTT TGGCCAGAAA TCATGCACTC AGGGAGAAGA TCCATTATAT TCGGACAGAG
    GGTACACATG GGCTTGAAAA GTTGTCCTGT GATGCAGATT TAGTAATTCT GCTGAGCCTT
    TTTGAAGAAG ATATCATGTC CTACATACCA CTCCAGGCCA CTTTCCATCC TGGTTACAGT
    TTCTCTCCTC GCTGCTCACC CTGCTCATCT CCCCAAAATT CTCCAGGACT GCAGCGAGCA
    AGTGCAAGAG CACCCTCTCC ATACAGGAGA GATTTTGAAG CCAAACTACG CAATTTCTAT
    CGAAAACTTG AAGCCAAAGG ATATGGACAA GGTCCAGGTA AAATTAAGTT AATTATACGA
    CGTGACCACT TACTGGAAGG CACCTTCAAC CAGGTGATGG CATATTCACG CAAGGAGCTC
    CAGCGAAACA AACTCTACAT CACATTTGTT GGCGAGGAAG GGCTGGACTA CAGTGGCCCC
    TCTCGAGAAT TCTTTTTCCT TCTGTCTCAG GAGCTCTTCA ATCCATATTA CGGGCTGTTT
    GAGTATTCAG CCAATGACAC TTACACTGTA CAGATTAGCC CCATGTCTGC CTTTGTTGAA
    AATCACCTGG AATGGTTCAG GTTCAGTGGT CGTATTCTTG GCCTTGCTCT CATTCATCAG
    TACCTTCTTG ATGCTTTCTT CACAAGGCCG TTCTACAAAG CACTATTAAG GCTGCCATGT
    GATCTAAGTG ACTTAGAGTA CCTGGATGAA GAATTCCATC AGAGCTTGCA GTGGATGAAA
    GATAACAACA TCACAGATAT CCTGGATCTC ACCTTCACAG TGAATGAGGA GGTGTTTGGA
    CAGGTGACAG AGAGAGAGTT GAAATCTGGA GGGGCAAATA CAGCAGTGAC AGAAAAGAAC
    AAAAAAGAGT ACATTGAGAG AATGGTTAAA TGGCGAGTGG AACGAGGAGT TGTTCAACAA
    ACAGAGGCCC TGGTCCGTGG CTTCTATGAA GTTGTAGACT CAAGGCTTGT CTCTGTTTTT
    GATGCCAGAG AACTGGAGCT GGTTATAGCT GGCACTGCAG AAATAGATCT CAATGACTGG
    CGTAACAACA CGGAATATCG TGGAGGTTAC CACGATGGAC ACATTGTAAT ACGGTGGTTC
    TGGGCAGCAG TGGAGAGGTT TAACAATGAA CAGAGACTCC GGTTGTTGCA GTTTGTTACT
    GGAACATCAA GTGTGCCATA TGAGGGCTTT GCAGCTCTCC GAGGAAGCAA TGGGCTTCGG
    CGTTTCTGTA TAGAGAAATG GGGGAAAATA ACCTCCCTTC CGAGGGCACA TACATGTTTC
    AACCGCTTGG ATCTTCCACC TTACCCCTCA TATTCTATGC TGTATGAAAA GCTGTTGACA
    GCTGTTGAAG AAACTAGTAC CTTTGGACTT GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa146112
    Clone ID Related Accession (Same CDS sequence) XM_025147587.1
    Accession Version XM_025147587.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAGTTA CAAGAATCCT GCAGTACATG GGCACGAGAT CCTGTAGGCC AATTTGGCAG 
    ATTTTTAAAA GCATCACCAA TGAAGATTCA ATCCAGGGAC AGGGAAATCG GAGGCTGATA
    AGTTTTTCAC TGTCAGATTT CCAAGCTTTT GGTCTGAAGA AAGGAATGTT TTTCAATCCA
    GATCCTTATC TGAAGATTTC CATCCAGCCT GGGAAACACA GCATCTTCCC AGCACTTCCT
    CATCATGGAC AGGAGAAAAG GTCTAAGATC ATGTGTAATA CAGTTAACCC AATCTGGCAA
    GGAGAGCAAT TCAGCTTTGT CTCACTGCCC ACGGATGTCC TGGAAATAGA AGTTAAAGAC
    AAATTTGCAA AGAGCCGACC AATCATCAAG CGTTTCCTGG GAAAACTCTC TATGCCAGTT
    CAGCGTCTCT TGGAAAGACA TGCCATAGGG GACAGGGTTG TAAGCTACAC TCTTGGTCGC
    AGGCTTCCTA CAGATCATGT CAGTGGCCAG CTGCAGTTCC GATTTGAGAT AACTTCTTCC
    ATTCATCAAG ATGATGAAGA GGTTACCATT AGTGCAGATC CTGAGCCAGC TGACCTCCAG
    GAAAGCCCTG TGAACAACCC CACAGAGGAA AGCCTCGGTG AGCCTCCATG CATCAACACA
    GTGACCTCAG AAAATACAGT TCCTGAAGAG CTAAATGGAT ACAGTGTGAA CAATATTGAT
    AGCCCAGGGG CTGTCAAACC ACCCAGGGAA GTCAACCAGA ACAACATAAA TGAAGAGCTA
    GCAGGAGATG AGGAGGTTGA TGCCATGCCA GTTCCTGAAC CCTTGGAATC CATCCGAGGA
    GAAGTGCTGC CTTCTTTGAA TGCGACAGAC CTCACAGAGC AGCTGGGTCT GCTGGCTTGC
    TTGTCTCCTT CTGAGACTGA AGTTAGGGAA AATAGCAAAG TCAATTCTGG TACTCAGGGA
    GATGATGGAG TATTGAACAG TACAGAAACT TTAGATGTTA GTGAGGTGAG TGCAGATGTA
    TATGCTGACA AAGACCTAGA GAAGAGTCAT GAAGAAGAAG GGGCTTCAGC CCGAGGGGAG
    GAAGACAGCA AGCTACAGCT GAGGACCACA GCAAAGAGGA AAACAAGGCC ATGCTCCCTG
    CCTGTGTCTG AAATTGAGAC CGTTATAGCT TCAGCTTGTG GTGAGCCAGA GACCCCTCGC
    ACTCACTATA TACGAATCCA CAACCTGCTC CACAGCCTGC CCTCAGCACA GATGAGCAGT
    GATGGGGAAG AAGAGCAAGA TGCTGGCAAT GGTGGGGAGA ATGATGAAGA GTCTACAGTC
    AAGGAAGTGT TGCAGAAGGA GACAGTCAGT GAAAGTGAGA CAATGGCAGC AGAGCCTCCT
    CCAGAAAATG AGGCCGAAGA GAACTTCAGC CAGTGCACAG TGCCTCCTGG GACCTTAGAC
    GAGCAACTCT CTGACTTAAA TGATGGACTG GTGGATGGGG AAAACCCTAG GACAGGAAGC
    GAAAGCGAGT CAAGCTCCAG ACCCAATGGT GACAACGAAT GTGAGGCGTG CGACACCTCT
    TGCTACAGCC CCTCCTGCTA TAGTACCTCC TGCTACAGTA CTTCCTGTTA CAGCACCTCT
    TGCTACAGCA CCTCCTGCTA TGACAGTAAC AATCGCTTCT CCAGCCATAC CCGCTTCTCC
    TCTGTTGACA GTGCAAAGAT TTCTGAGAGC ACGGTCTTTT CCTCACAGGA TGAGGAGGAG
    GAGAATAGTG CTTTTGAATC GGTGCCAGAC TCAGTACAGA GCCCTGAGCT GGACAGGGAG
    CAAGTGGATG GCTCCTCCCA GTGGCCAGAT GAACTAGTAG CTCATGGTGG GAATCCACAA
    CTAGCCGCAG AAAGTCTAGA CACCCCAATA GCAGGTCCAA GCAGCAGAAG AGAAGGTGAT
    TGTCCTTTAC TCCATAATTC TCAGCCAGTC AGCCAACTTC CTTCTCTGAG GCCTGACCAT
    CATCACTACC CAACTATCGA TGAGCCTCTT CCACCAACTG CAACATCCTT GAGAAACCTT
    ACCCAGTTGG ATAACCAGAG AGCCCTCCCT TTAAAATTAT GTGATGTTCA CAGAGAGACT
    GTATATTGGG AAGCTCGGAT AGACAGCCAC GGGAGGGTGT TCTACGTTGA CCATGTCAAC
    CGAACTACCA CATGGCAGCG CCCCACAGCT GCAGCAACAC CAGATGGGAT CCACAGGTCT
    GGCTCTATCC AGCAAATGGA GCAGCTGAAC CGGCGATATC AAAATATCCA GCGAACTATT
    GCAACAGAGC GGACTGAAGA TGATGGTGTA GTAAACAACA GAGTGGAGAG AATTCCCACT
    GGAGGAGGAA GTGACTCTGA GGCAGAGCCT TCCCAACCAA GCTCCGAAAT TAGGAGGGAA
    GGTTCCCTTT CTCCTGTGAA TTCTCAAAAA ATCACCTTAT TGCTGCAGTC TCCAGCTGTG
    AAGTTTATCA CCAATCCTGA GTTCTTCACT GTGCTGCATG CAAACTATAG TGCCTATCGA
    GTCTTCACTA ACAGTACCTG CTTGAAGCAT ATGATCCTGA AGATCCGTAG AGATGCTCGT
    AATTTTGAGC GCTATCAGCA CAACAGAGAC CTTGTAAATT TTATCAACAT GTTTGCTGAC
    ACTCGACTAG AGCTTCCTCG TGGCTGGGAA ATAAAAACTG ACCAGCAGGG CAAGTCTTTC
    TTCGTTGACC ACAACAGTCG TGCTACCACA TTCATAGATC CCAGGATCCC ACTGCAGAAT
    GGTCGTCTCC CCAATCACTT GACTCACAGG CAACATCTTC AGCGGCTTCG TAGCTACAGT
    GCTGGAGAGG CTTCTGAAGT CTCTCGGAAC AGAGGAGTTA CTCTGTTGGC CAGACCAGGC
    AATAGTCTTG TAACAGCTAT TCGAAACCAG CATCATTATG AGACATTACC TCTGGCTTAC
    AATGACAAGA TTGTTGCATT TCTACGCCAA CCTAACATTT TTGAGATGCT GCAAGAGCGT
    CAGCCCAGTT TGGCCAGAAA TCATGCACTC AGGGAGAAGA TCCATTATAT TCGGACAGAG
    GGTACACATG GGCTTGAAAA GTTGTCCTGT GATGCAGATT TAGTAATTCT GCTGAGCCTT
    TTTGAAGAAG ATATCATGTC CTACATACCA CTCCAGGCCA CTTTCCATCC TGGTTACAGT
    TTCTCTCCTC GCTGCTCACC CTGCTCATCT CCCCAAAATT CTCCAGGACT GCAGCGAGCA
    AGTGCAAGAG CACCCTCTCC ATACAGGAGA GATTTTGAAG CCAAACTACG CAATTTCTAT
    CGAAAACTTG AAGCCAAAGG ATATGGACAA GGTCCAGGTA AAATTAAGTT AATTATACGA
    CGTGACCACT TACTGGAAGG CACCTTCAAC CAGGTGATGG CATATTCACG CAAGGAGCTC
    CAGCGAAACA AACTCTACAT CACATTTGTT GGCGAGGAAG GGCTGGACTA CAGTGGCCCC
    TCTCGAGAAT TCTTTTTCCT TCTGTCTCAG GAGCTCTTCA ATCCATATTA CGGGCTGTTT
    GAGTATTCAG CCAATGACAC TTACACTGTA CAGATTAGCC CCATGTCTGC CTTTGTTGAA
    AATCACCTGG AATGGTTCAG GTTCAGTGGT CGTATTCTTG GCCTTGCTCT CATTCATCAG
    TACCTTCTTG ATGCTTTCTT CACAAGGCCG TTCTACAAAG CACTATTAAG GCTGCCATGT
    GATCTAAGTG ACTTAGAGTA CCTGGATGAA GAATTCCATC AGAGCTTGCA GTGGATGAAA
    GATAACAACA TCACAGATAT CCTGGATCTC ACCTTCACAG TGAATGAGGA GGTGTTTGGA
    CAGGTGACAG AGAGAGAGTT GAAATCTGGA GGGGCAAATA CAGCAGTGAC AGAAAAGAAC
    AAAAAAGAGT ACATTGAGAG AATGGTTAAA TGGCGAGTGG AACGAGGAGT TGTTCAACAA
    ACAGAGGCCC TGGTCCGTGG CTTCTATGAA GTTGTAGACT CAAGGCTTGT CTCTGTTTTT
    GATGCCAGAG AACTGGAGCT GGTTATAGCT GGCACTGCAG AAATAGATCT CAATGACTGG
    CGTAACAACA CGGAATATCG TGGAGGTTAC CACGATGGAC ACATTGTAAT ACGGTGGTTC
    TGGGCAGCAG TGGAGAGGTT TAACAATGAA CAGAGACTCC GGTTGTTGCA GTTTGTTACT
    GGAACATCAA GTGTGCCATA TGAGGGCTTT GCAGCTCTCC GAGGAAGCAA TGGGCTTCGG
    CGTTTCTGTA TAGAGAAATG GGGGAAAATA ACCTCCCTTC CGAGGGCACA TACATGTTTC
    AACCGCTTGG ATCTTCCACC TTACCCCTCA TATTCTATGC TGTATGAAAA GCTGTTGACA
    GCTGTTGAAG AAACTAGTAC CTTTGGACTT GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025003355.1
    CDS32..4447
    Translation

    Target ORF information:

    RefSeq Version XM_025147587.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025147587.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAGTTA CAAGAATCCT GCAGTACATG GGCACGAGAT CCTGTAGGCC AATTTGGCAG 
    ATTTTTAAAA GCATCACCAA TGAAGATTCA ATCCAGGGAC AGGGAAATCG GAGGCTGATA
    AGTTTTTCAC TGTCAGATTT CCAAGCTTTT GGTCTGAAGA AAGGAATGTT TTTCAATCCA
    GATCCTTATC TGAAGATTTC CATCCAGCCT GGGAAACACA GCATCTTCCC AGCACTTCCT
    CATCATGGAC AGGAGAAAAG GTCTAAGATC ATGTGTAATA CAGTTAACCC AATCTGGCAA
    GGAGAGCAAT TCAGCTTTGT CTCACTGCCC ACGGATGTCC TGGAAATAGA AGTTAAAGAC
    AAATTTGCAA AGAGCCGACC AATCATCAAG CGTTTCCTGG GAAAACTCTC TATGCCAGTT
    CAGCGTCTCT TGGAAAGACA TGCCATAGGG GACAGGGTTG TAAGCTACAC TCTTGGTCGC
    AGGCTTCCTA CAGATCATGT CAGTGGCCAG CTGCAGTTCC GATTTGAGAT AACTTCTTCC
    ATTCATCAAG ATGATGAAGA GGTTACCATT AGTGCAGATC CTGAGCCAGC TGACCTCCAG
    GAAAGCCCTG TGAACAACCC CACAGAGGAA AGCCTCGGTG AGCCTCCATG CATCAACACA
    GTGACCTCAG AAAATACAGT TCCTGAAGAG CTAAATGGAT ACAGTGTGAA CAATATTGAT
    AGCCCAGGGG CTGTCAAACC ACCCAGGGAA GTCAACCAGA ACAACATAAA TGAAGAGCTA
    GCAGGAGATG AGGAGGTTGA TGCCATGCCA GTTCCTGAAC CCTTGGAATC CATCCGAGGA
    GAAGTGCTGC CTTCTTTGAA TGCGACAGAC CTCACAGAGC AGCTGGGTCT GCTGGCTTGC
    TTGTCTCCTT CTGAGACTGA AGTTAGGGAA AATAGCAAAG TCAATTCTGG TACTCAGGGA
    GATGATGGAG TATTGAACAG TACAGAAACT TTAGATGTTA GTGAGGTGAG TGCAGATGTA
    TATGCTGACA AAGACCTAGA GAAGAGTCAT GAAGAAGAAG GGGCTTCAGC CCGAGGGGAG
    GAAGACAGCA AGCTACAGCT GAGGACCACA GCAAAGAGGA AAACAAGGCC ATGCTCCCTG
    CCTGTGTCTG AAATTGAGAC CGTTATAGCT TCAGCTTGTG GTGAGCCAGA GACCCCTCGC
    ACTCACTATA TACGAATCCA CAACCTGCTC CACAGCCTGC CCTCAGCACA GATGAGCAGT
    GATGGGGAAG AAGAGCAAGA TGCTGGCAAT GGTGGGGAGA ATGATGAAGA GTCTACAGTC
    AAGGAAGTGT TGCAGAAGGA GACAGTCAGT GAAAGTGAGA CAATGGCAGC AGAGCCTCCT
    CCAGAAAATG AGGCCGAAGA GAACTTCAGC CAGTGCACAG TGCCTCCTGG GACCTTAGAC
    GAGCAACTCT CTGACTTAAA TGATGGACTG GTGGATGGGG AAAACCCTAG GACAGGAAGC
    GAAAGCGAGT CAAGCTCCAG ACCCAATGGT GACAACGAAT GTGAGGCGTG CGACACCTCT
    TGCTACAGCC CCTCCTGCTA TAGTACCTCC TGCTACAGTA CTTCCTGTTA CAGCACCTCT
    TGCTACAGCA CCTCCTGCTA TGACAGTAAC AATCGCTTCT CCAGCCATAC CCGCTTCTCC
    TCTGTTGACA GTGCAAAGAT TTCTGAGAGC ACGGTCTTTT CCTCACAGGA TGAGGAGGAG
    GAGAATAGTG CTTTTGAATC GGTGCCAGAC TCAGTACAGA GCCCTGAGCT GGACAGGGAG
    CAAGTGGATG GCTCCTCCCA GTGGCCAGAT GAACTAGTAG CTCATGGTGG GAATCCACAA
    CTAGCCGCAG AAAGTCTAGA CACCCCAATA GCAGGTCCAA GCAGCAGAAG AGAAGGTGAT
    TGTCCTTTAC TCCATAATTC TCAGCCAGTC AGCCAACTTC CTTCTCTGAG GCCTGACCAT
    CATCACTACC CAACTATCGA TGAGCCTCTT CCACCAACTG CAACATCCTT GAGAAACCTT
    ACCCAGTTGG ATAACCAGAG AGCCCTCCCT TTAAAATTAT GTGATGTTCA CAGAGAGACT
    GTATATTGGG AAGCTCGGAT AGACAGCCAC GGGAGGGTGT TCTACGTTGA CCATGTCAAC
    CGAACTACCA CATGGCAGCG CCCCACAGCT GCAGCAACAC CAGATGGGAT CCACAGGTCT
    GGCTCTATCC AGCAAATGGA GCAGCTGAAC CGGCGATATC AAAATATCCA GCGAACTATT
    GCAACAGAGC GGACTGAAGA TGATGGTGTA GTAAACAACA GAGTGGAGAG AATTCCCACT
    GGAGGAGGAA GTGACTCTGA GGCAGAGCCT TCCCAACCAA GCTCCGAAAT TAGGAGGGAA
    GGTTCCCTTT CTCCTGTGAA TTCTCAAAAA ATCACCTTAT TGCTGCAGTC TCCAGCTGTG
    AAGTTTATCA CCAATCCTGA GTTCTTCACT GTGCTGCATG CAAACTATAG TGCCTATCGA
    GTCTTCACTA ACAGTACCTG CTTGAAGCAT ATGATCCTGA AGATCCGTAG AGATGCTCGT
    AATTTTGAGC GCTATCAGCA CAACAGAGAC CTTGTAAATT TTATCAACAT GTTTGCTGAC
    ACTCGACTAG AGCTTCCTCG TGGCTGGGAA ATAAAAACTG ACCAGCAGGG CAAGTCTTTC
    TTCGTTGACC ACAACAGTCG TGCTACCACA TTCATAGATC CCAGGATCCC ACTGCAGAAT
    GGTCGTCTCC CCAATCACTT GACTCACAGG CAACATCTTC AGCGGCTTCG TAGCTACAGT
    GCTGGAGAGG CTTCTGAAGT CTCTCGGAAC AGAGGAGTTA CTCTGTTGGC CAGACCAGGC
    AATAGTCTTG TAACAGCTAT TCGAAACCAG CATCATTATG AGACATTACC TCTGGCTTAC
    AATGACAAGA TTGTTGCATT TCTACGCCAA CCTAACATTT TTGAGATGCT GCAAGAGCGT
    CAGCCCAGTT TGGCCAGAAA TCATGCACTC AGGGAGAAGA TCCATTATAT TCGGACAGAG
    GGTACACATG GGCTTGAAAA GTTGTCCTGT GATGCAGATT TAGTAATTCT GCTGAGCCTT
    TTTGAAGAAG ATATCATGTC CTACATACCA CTCCAGGCCA CTTTCCATCC TGGTTACAGT
    TTCTCTCCTC GCTGCTCACC CTGCTCATCT CCCCAAAATT CTCCAGGACT GCAGCGAGCA
    AGTGCAAGAG CACCCTCTCC ATACAGGAGA GATTTTGAAG CCAAACTACG CAATTTCTAT
    CGAAAACTTG AAGCCAAAGG ATATGGACAA GGTCCAGGTA AAATTAAGTT AATTATACGA
    CGTGACCACT TACTGGAAGG CACCTTCAAC CAGGTGATGG CATATTCACG CAAGGAGCTC
    CAGCGAAACA AACTCTACAT CACATTTGTT GGCGAGGAAG GGCTGGACTA CAGTGGCCCC
    TCTCGAGAAT TCTTTTTCCT TCTGTCTCAG GAGCTCTTCA ATCCATATTA CGGGCTGTTT
    GAGTATTCAG CCAATGACAC TTACACTGTA CAGATTAGCC CCATGTCTGC CTTTGTTGAA
    AATCACCTGG AATGGTTCAG GTTCAGTGGT CGTATTCTTG GCCTTGCTCT CATTCATCAG
    TACCTTCTTG ATGCTTTCTT CACAAGGCCG TTCTACAAAG CACTATTAAG GCTGCCATGT
    GATCTAAGTG ACTTAGAGTA CCTGGATGAA GAATTCCATC AGAGCTTGCA GTGGATGAAA
    GATAACAACA TCACAGATAT CCTGGATCTC ACCTTCACAG TGAATGAGGA GGTGTTTGGA
    CAGGTGACAG AGAGAGAGTT GAAATCTGGA GGGGCAAATA CAGCAGTGAC AGAAAAGAAC
    AAAAAAGAGT ACATTGAGAG AATGGTTAAA TGGCGAGTGG AACGAGGAGT TGTTCAACAA
    ACAGAGGCCC TGGTCCGTGG CTTCTATGAA GTTGTAGACT CAAGGCTTGT CTCTGTTTTT
    GATGCCAGAG AACTGGAGCT GGTTATAGCT GGCACTGCAG AAATAGATCT CAATGACTGG
    CGTAACAACA CGGAATATCG TGGAGGTTAC CACGATGGAC ACATTGTAAT ACGGTGGTTC
    TGGGCAGCAG TGGAGAGGTT TAACAATGAA CAGAGACTCC GGTTGTTGCA GTTTGTTACT
    GGAACATCAA GTGTGCCATA TGAGGGCTTT GCAGCTCTCC GAGGAAGCAA TGGGCTTCGG
    CGTTTCTGTA TAGAGAAATG GGGGAAAATA ACCTCCCTTC CGAGGGCACA TACATGTTTC
    AACCGCTTGG ATCTTCCACC TTACCCCTCA TATTCTATGC TGTATGAAAA GCTGTTGACA
    GCTGTTGAAG AAACTAGTAC CTTTGGACTT GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa146113
    Clone ID Related Accession (Same CDS sequence) XM_025147588.1
    Accession Version XM_025147588.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGGGATG TACTGGAAGA AATACAGCTA CCGGTTATGA AAGTCCCTCA GAATCTGTAC 
    CAAAACAGGT TCCTGGGCCT CGCAGCCATG GCGTCTCCAT CTAGGAACTC ACAAAATCGG
    CGCCGGTGCA AAGAGCCACT CAGATACAGC TATAACCCTG ACCAGTTTCA TAACATGGAC
    ATCAGGAACA ATTCCCATGA GACAGTCACC ATTCCTAGGT CTACCAGTGA CACGGACCTT
    GTCACTTCAG ACAGCCGATC TACCCTGATG GTCAGCAGCT CCTACTATTC CATTGGGCAC
    TCACAGGACC TAGTGATATA CTGGGATATA AAGGAGGAAG TGGATGCAGG GGACTGGATT
    GGCATGTATC TCATAGATGA AGTCTTGTCT GAAAACTTTT TGGACTACAA GAACCGTGGA
    GTCAATGGCT CTCATCGTGG TCAGATTGTC TGGAAGATTG ATGCCAGTTC TTACTTTGTG
    GAGCCTGAAA CAAAGATATG CTTCAAATAC TATCACGGTG TGAGTGGGGC ACTACGAGCC
    ACAACTCCAA GCGTCACGGT GAAAAACAAT GCAGCTCCTC AGATTTTTAA AAGCATCACC
    AATGAAGATT CAATCCAGGG ACAGGGAAAT CGGAGGCTGA TAAGTTTTTC ACTGTCAGAT
    TTCCAAGCTT TTGGTCTGAA GAAAGGAATG TTTTTCAATC CAGATCCTTA TCTGAAGATT
    TCCATCCAGC CTGGGAAACA CAGCATCTTC CCAGCACTTC CTCATCATGG ACAGGAGAAA
    AGGTCTAAGA TCATGTGTAA TACAGTTAAC CCAATCTGGC AAGGAGAGCA ATTCAGCTTT
    GTCTCACTGC CCACGGATGT CCTGGAAATA GAAGTTAAAG ACAAATTTGC AAAGAGCCGA
    CCAATCATCA AGCGTTTCCT GGGAAAACTC TCTATGCCAG TTCAGCGTCT CTTGGAAAGA
    CATGCCATAG GGGACAGGGT TGTAAGCTAC ACTCTTGGTC GCAGGCTTCC TACAGATCAT
    GTCAGTGGCC AGCTGCAGTT CCGATTTGAG ATAACTTCTT CCATTCATCA AGATGATGAA
    GAGGTTACCA TTAGTGCAGA TCCTGAGCCA GCTGACCTCC AGGAAAGCCC TGTGAACAAC
    CCCACAGAGG AAAGCCTCGG TGATTGTCCT TTACTCCATA ATTCTCAGCC AGTCAGCCAA
    CTTCCTTCTC TGAGGCCTGA CCATCATCAC TACCCAACTA TCGATGAGCC TCTTCCACCA
    AATTGGGAAG CTCGGATAGA CAGCCACGGG AGGGTGTTCT ACGTTGACCA TGTCAACCGA
    ACTACCACAT GGCAGCGCCC CACAGCTGCA GCAACACCAG ATGGGATCCA CAGGTCTGGC
    TCTATCCAGC AAATGGAGCA GCTGAACCGG CGATATCAAA ATATCCAGCG AACTATTGCA
    ACAGAGCGGA CTGAAGATGA TGGTGTAGTA AACAACAGAG TGGAGAGAAT TCCCACTGGA
    GGAGGAAGTG ACTCTGAGGC AGAGCCTTCC CAACCAAGCT CCGAAATTAG GAGGGAAGGT
    TCCCTTTCTC CTGTGAATTC TCAAAAAATC ACCTTATTGC TGCAGTCTCC AGCTGTGAAG
    TTTATCACCA ATCCTGAGTT CTTCACTGTG CTGCATGCAA ACTATAGTGC CTATCGAGTC
    TTCACTAACA GTACCTGCTT GAAGCATATG ATCCTGAAGA TCCGTAGAGA TGCTCGTAAT
    TTTGAGCGCT ATCAGCACAA CAGAGACCTT GTAAATTTTA TCAACATGTT TGCTGACACT
    CGACTAGAGC TTCCTCGTGG CTGGGAAATA AAAACTGACC AGCAGGGCAA GTCTTTCTTC
    GTTGACCACA ACAGTCGTGC TACCACATTC ATAGATCCCA GGATCCCACT GCAGAATGGT
    CGTCTCCCCA ATCACTTGAC TCACAGGCAA CATCTTCAGC GGCTTCGTAG CTACAGTGCT
    GGAGAGGCTT CTGAAGTCTC TCGGAACAGA GGAGTTACTC TGTTGGCCAG ACCAGGCAAT
    AGTCTTGTAA CAGCTATTCG AAACCAGCAT CATTATGAGA CATTACCTCT GGCTTACAAT
    GACAAGATTG TTGCATTTCT ACGCCAACCT AACATTTTTG AGATGCTGCA AGAGCGTCAG
    CCCAGTTTGG CCAGAAATCA TGCACTCAGG GAGAAGATCC ATTATATTCG GACAGAGGGT
    ACACATGGGC TTGAAAAGTT GTCCTGTGAT GCAGATTTAG TAATTCTGCT GAGCCTTTTT
    GAAGAAGATA TCATGTCCTA CATACCACTC CAGGCCACTT TCCATCCTGG TTACAGTTTC
    TCTCCTCGCT GCTCACCCTG CTCATCTCCC CAAAATTCTC CAGGACTGCA GCGAGCAAGT
    GCAAGAGCAC CCTCTCCATA CAGGAGAGAT TTTGAAGCCA AACTACGCAA TTTCTATCGA
    AAACTTGAAG CCAAAGGATA TGGACAAGGT CCAGGTAAAA TTAAGTTAAT TATACGACGT
    GACCACTTAC TGGAAGGCAC CTTCAACCAG GTGATGGCAT ATTCACGCAA GGAGCTCCAG
    CGAAACAAAC TCTACATCAC ATTTGTTGGC GAGGAAGGGC TGGACTACAG TGGCCCCTCT
    CGAGAATTCT TTTTCCTTCT GTCTCAGGAG CTCTTCAATC CATATTACGG GCTGTTTGAG
    TATTCAGCCA ATGACACTTA CACTGTACAG ATTAGCCCCA TGTCTGCCTT TGTTGAAAAT
    CACCTGGAAT GGTTCAGGTT CAGTGGTCGT ATTCTTGGCC TTGCTCTCAT TCATCAGTAC
    CTTCTTGATG CTTTCTTCAC AAGGCCGTTC TACAAAGCAC TATTAAGGCT GCCATGTGAT
    CTAAGTGACT TAGAGTACCT GGATGAAGAA TTCCATCAGA GCTTGCAGTG GATGAAAGAT
    AACAACATCA CAGATATCCT GGATCTCACC TTCACAGTGA ATGAGGAGGT GTTTGGACAG
    GTGACAGAGA GAGAGTTGAA ATCTGGAGGG GCAAATACAG CAGTGACAGA AAAGAACAAA
    AAAGAGTACA TTGAGAGAAT GGTTAAATGG CGAGTGGAAC GAGGAGTTGT TCAACAAACA
    GAGGCCCTGG TCCGTGGCTT CTATGAAGTT GTAGACTCAA GGCTTGTCTC TGTTTTTGAT
    GCCAGAGAAC TGGAGCTGGT TATAGCTGGC ACTGCAGAAA TAGATCTCAA TGACTGGCGT
    AACAACACGG AATATCGTGG AGGTTACCAC GATGGACACA TTGTAATACG GTGGTTCTGG
    GCAGCAGTGG AGAGGTTTAA CAATGAACAG AGACTCCGGT TGTTGCAGTT TGTTACTGGA
    ACATCAAGTG TGCCATATGA GGGCTTTGCA GCTCTCCGAG GAAGCAATGG GCTTCGGCGT
    TTCTGTATAG AGAAATGGGG GAAAATAACC TCCCTTCCGA GGGCACATAC ATGTTTCAAC
    CGCTTGGATC TTCCACCTTA CCCCTCATAT TCTATGCTGT ATGAAAAGCT GTTGACAGCT
    GTTGAAGAAA CTAGTACCTT TGGACTTGAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025003356.1
    CDS166..3738
    Translation

    Target ORF information:

    RefSeq Version XM_025147588.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025147588.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGGGATG TACTGGAAGA AATACAGCTA CCGGTTATGA AAGTCCCTCA GAATCTGTAC 
    CAAAACAGGT TCCTGGGCCT CGCAGCCATG GCGTCTCCAT CTAGGAACTC ACAAAATCGG
    CGCCGGTGCA AAGAGCCACT CAGATACAGC TATAACCCTG ACCAGTTTCA TAACATGGAC
    ATCAGGAACA ATTCCCATGA GACAGTCACC ATTCCTAGGT CTACCAGTGA CACGGACCTT
    GTCACTTCAG ACAGCCGATC TACCCTGATG GTCAGCAGCT CCTACTATTC CATTGGGCAC
    TCACAGGACC TAGTGATATA CTGGGATATA AAGGAGGAAG TGGATGCAGG GGACTGGATT
    GGCATGTATC TCATAGATGA AGTCTTGTCT GAAAACTTTT TGGACTACAA GAACCGTGGA
    GTCAATGGCT CTCATCGTGG TCAGATTGTC TGGAAGATTG ATGCCAGTTC TTACTTTGTG
    GAGCCTGAAA CAAAGATATG CTTCAAATAC TATCACGGTG TGAGTGGGGC ACTACGAGCC
    ACAACTCCAA GCGTCACGGT GAAAAACAAT GCAGCTCCTC AGATTTTTAA AAGCATCACC
    AATGAAGATT CAATCCAGGG ACAGGGAAAT CGGAGGCTGA TAAGTTTTTC ACTGTCAGAT
    TTCCAAGCTT TTGGTCTGAA GAAAGGAATG TTTTTCAATC CAGATCCTTA TCTGAAGATT
    TCCATCCAGC CTGGGAAACA CAGCATCTTC CCAGCACTTC CTCATCATGG ACAGGAGAAA
    AGGTCTAAGA TCATGTGTAA TACAGTTAAC CCAATCTGGC AAGGAGAGCA ATTCAGCTTT
    GTCTCACTGC CCACGGATGT CCTGGAAATA GAAGTTAAAG ACAAATTTGC AAAGAGCCGA
    CCAATCATCA AGCGTTTCCT GGGAAAACTC TCTATGCCAG TTCAGCGTCT CTTGGAAAGA
    CATGCCATAG GGGACAGGGT TGTAAGCTAC ACTCTTGGTC GCAGGCTTCC TACAGATCAT
    GTCAGTGGCC AGCTGCAGTT CCGATTTGAG ATAACTTCTT CCATTCATCA AGATGATGAA
    GAGGTTACCA TTAGTGCAGA TCCTGAGCCA GCTGACCTCC AGGAAAGCCC TGTGAACAAC
    CCCACAGAGG AAAGCCTCGG TGATTGTCCT TTACTCCATA ATTCTCAGCC AGTCAGCCAA
    CTTCCTTCTC TGAGGCCTGA CCATCATCAC TACCCAACTA TCGATGAGCC TCTTCCACCA
    AATTGGGAAG CTCGGATAGA CAGCCACGGG AGGGTGTTCT ACGTTGACCA TGTCAACCGA
    ACTACCACAT GGCAGCGCCC CACAGCTGCA GCAACACCAG ATGGGATCCA CAGGTCTGGC
    TCTATCCAGC AAATGGAGCA GCTGAACCGG CGATATCAAA ATATCCAGCG AACTATTGCA
    ACAGAGCGGA CTGAAGATGA TGGTGTAGTA AACAACAGAG TGGAGAGAAT TCCCACTGGA
    GGAGGAAGTG ACTCTGAGGC AGAGCCTTCC CAACCAAGCT CCGAAATTAG GAGGGAAGGT
    TCCCTTTCTC CTGTGAATTC TCAAAAAATC ACCTTATTGC TGCAGTCTCC AGCTGTGAAG
    TTTATCACCA ATCCTGAGTT CTTCACTGTG CTGCATGCAA ACTATAGTGC CTATCGAGTC
    TTCACTAACA GTACCTGCTT GAAGCATATG ATCCTGAAGA TCCGTAGAGA TGCTCGTAAT
    TTTGAGCGCT ATCAGCACAA CAGAGACCTT GTAAATTTTA TCAACATGTT TGCTGACACT
    CGACTAGAGC TTCCTCGTGG CTGGGAAATA AAAACTGACC AGCAGGGCAA GTCTTTCTTC
    GTTGACCACA ACAGTCGTGC TACCACATTC ATAGATCCCA GGATCCCACT GCAGAATGGT
    CGTCTCCCCA ATCACTTGAC TCACAGGCAA CATCTTCAGC GGCTTCGTAG CTACAGTGCT
    GGAGAGGCTT CTGAAGTCTC TCGGAACAGA GGAGTTACTC TGTTGGCCAG ACCAGGCAAT
    AGTCTTGTAA CAGCTATTCG AAACCAGCAT CATTATGAGA CATTACCTCT GGCTTACAAT
    GACAAGATTG TTGCATTTCT ACGCCAACCT AACATTTTTG AGATGCTGCA AGAGCGTCAG
    CCCAGTTTGG CCAGAAATCA TGCACTCAGG GAGAAGATCC ATTATATTCG GACAGAGGGT
    ACACATGGGC TTGAAAAGTT GTCCTGTGAT GCAGATTTAG TAATTCTGCT GAGCCTTTTT
    GAAGAAGATA TCATGTCCTA CATACCACTC CAGGCCACTT TCCATCCTGG TTACAGTTTC
    TCTCCTCGCT GCTCACCCTG CTCATCTCCC CAAAATTCTC CAGGACTGCA GCGAGCAAGT
    GCAAGAGCAC CCTCTCCATA CAGGAGAGAT TTTGAAGCCA AACTACGCAA TTTCTATCGA
    AAACTTGAAG CCAAAGGATA TGGACAAGGT CCAGGTAAAA TTAAGTTAAT TATACGACGT
    GACCACTTAC TGGAAGGCAC CTTCAACCAG GTGATGGCAT ATTCACGCAA GGAGCTCCAG
    CGAAACAAAC TCTACATCAC ATTTGTTGGC GAGGAAGGGC TGGACTACAG TGGCCCCTCT
    CGAGAATTCT TTTTCCTTCT GTCTCAGGAG CTCTTCAATC CATATTACGG GCTGTTTGAG
    TATTCAGCCA ATGACACTTA CACTGTACAG ATTAGCCCCA TGTCTGCCTT TGTTGAAAAT
    CACCTGGAAT GGTTCAGGTT CAGTGGTCGT ATTCTTGGCC TTGCTCTCAT TCATCAGTAC
    CTTCTTGATG CTTTCTTCAC AAGGCCGTTC TACAAAGCAC TATTAAGGCT GCCATGTGAT
    CTAAGTGACT TAGAGTACCT GGATGAAGAA TTCCATCAGA GCTTGCAGTG GATGAAAGAT
    AACAACATCA CAGATATCCT GGATCTCACC TTCACAGTGA ATGAGGAGGT GTTTGGACAG
    GTGACAGAGA GAGAGTTGAA ATCTGGAGGG GCAAATACAG CAGTGACAGA AAAGAACAAA
    AAAGAGTACA TTGAGAGAAT GGTTAAATGG CGAGTGGAAC GAGGAGTTGT TCAACAAACA
    GAGGCCCTGG TCCGTGGCTT CTATGAAGTT GTAGACTCAA GGCTTGTCTC TGTTTTTGAT
    GCCAGAGAAC TGGAGCTGGT TATAGCTGGC ACTGCAGAAA TAGATCTCAA TGACTGGCGT
    AACAACACGG AATATCGTGG AGGTTACCAC GATGGACACA TTGTAATACG GTGGTTCTGG
    GCAGCAGTGG AGAGGTTTAA CAATGAACAG AGACTCCGGT TGTTGCAGTT TGTTACTGGA
    ACATCAAGTG TGCCATATGA GGGCTTTGCA GCTCTCCGAG GAAGCAATGG GCTTCGGCGT
    TTCTGTATAG AGAAATGGGG GAAAATAACC TCCCTTCCGA GGGCACATAC ATGTTTCAAC
    CGCTTGGATC TTCCACCTTA CCCCTCATAT TCTATGCTGT ATGAAAAGCT GTTGACAGCT
    GTTGAAGAAA CTAGTACCTT TGGACTTGAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa31690
    Clone ID Related Accession (Same CDS sequence) XM_015281615.1 , XM_015281615.2
    Accession Version XM_015281615.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACTGTG CCCAGGCCGG CATTGTCCAG AATCTGTACC AAAACAGGTT CCTGGGCCTC 
    GCAGCCATGG CGTCTCCATC TAGGAACTCA CAAAATCGGC GCCGGTGCAA AGAGCCACTC
    AGATACAGCT ATAACCCTGA CCAGTTTCAT AACATGGACA TCAGGAACAA TTCCCATGAG
    ACAGTCACCA TTCCTAGGTC TACCAGTGAC ACGGACCTTG TCACTTCAGA CAGCCGATCT
    ACCCTGATGG TCAGCAGCTC CTACTATTCC ATTGGGCACT CACAGGACCT AGTGATATAC
    TGGGATATAA AGGAGGAAGT GGATGCAGGG GACTGGATTG GCATGTATCT CATAGATGAA
    GTCTTGTCTG AAAACTTTTT GGACTACAAG AACCGTGGAG TCAATGGCTC TCATCGTGGT
    CAGATTGTCT GGAAGATTGA TGCCAGTTCT TACTTTGTGG AGCCTGAAAC AAAGATATGC
    TTCAAATACT ATCACGGTGT GAGTGGGGCA CTACGAGCCA CAACTCCAAG CGTCACGGTG
    AAAAACAATG CAGCTCCTCA GATTTTTAAA AGCATCACCA ATGAAGATTC AATCCAGGGA
    CAGGGAAATC GGAGGCTGAT AAGTTTTTCA CTGTCAGATT TCCAAGCTTT TGGTCTGAAG
    AAAGGAATGT TTTTCAATCC AGATCCTTAT CTGAAGATTT CCATCCAGCC TGGGAAACAC
    AGCATCTTCC CAGCACTTCC TCATCATGGA CAGGAGAAAA GGTCTAAGAT CATGTGTAAT
    ACAGTTAACC CAATCTGGCA AGGAGAGCAA TTCAGCTTTG TCTCACTGCC CACGGATGTC
    CTGGAAATAG AAGTTAAAGA CAAATTTGCA AAGAGCCGAC CAATCATCAA GCGTTTCCTG
    GGAAAACTCT CTATGCCAGT TCAGCGTCTC TTGGAAAGAC ATGCCATAGG GGACAGGGTT
    GTAAGCTACA CTCTTGGTCG CAGGCTTCCT ACAGATCATG TCAGTGGCCA GCTGCAGTTC
    CGATTTGAGA TAACTTCTTC CATTCATCAA GATGATGAAG AGGTTACCAT TAGTGCAGAT
    CCTGAGCCAG CTGACCTCCA GGAAAGCCCT GTGAACAACC CCACAGAGGA AAGCCTCGGT
    GAGCCTCCAT GCATCAACAC AGTGACCTCA GAAAATACAG TTCCTGAAGA GCTAAATGGA
    TACAGTGTGA ACAATATTGA TAGCCCAGGG GCTGTCAAAC CACCCAGGGA AGTCAACCAG
    AACAACATAA ATGAAGAGCT AGCAGGAGAT GAGGAGGTTG ATGCCATGCC AGTTCCTGAA
    CCCTTGGAAT CCATCCGAGG AGAAGTGCTG CCTTCTTTGA ATGCGACAGA CCTCACAGAG
    CAGCTGGGTC TGCTGGCTTG CTTGTCTCCT TCTGAGACTG AAGTTAGGGA AAATAGCAAA
    GTCAATTCTG GTACTCAGGG AGATGATGGA GTATTGAACA GTACAGAAAC TTTAGATGTT
    AGTGAGGTGA GTGCAGATGT ATATGCTGAC AAAGACCTAG AGAAGAGTCA TGAAGAAGAA
    GGGGCTTCAG CCCGAGGGGA GGAAGACAGC AAGCTACAGC TGAGGACCAC AGCAAAGAGG
    AAAACAAGGC CATGCTCCCT GCCTGTGTCT GAAATTGAGA CCGTTATAGC TTCAGCTTGT
    GGTGAGCCAG AGACCCCTCG CACTCACTAT ATACGAATCC ACAACCTGCT CCACAGCCTG
    CCCTCAGCAC AGATGAGCAG TGATGGGGAA GAAGAGCAAG ATGCTGGCAA TGGTGGGGAG
    AATGATGAAG AGTCTACAGT CAAGGAAGTG TTGCAGAAGG AGACAGTCAG TGAAAGTGAG
    ACAATGGCAG CAGAGCCTCC TCCAGAAAAT GAGGCCGAAG AGAACTTCAG CCAGTGCACA
    GTGCCTCCTG GGACCTTAGA CGAGCAACTC TCTGACTTAA ATGATGGACT GGTGGATGGG
    GAAAACCCTA GGACAGGAAG CGAAAGCGAG TCAAGCTCCA GACCCAATGG TGACAACGAA
    TGTGAGGCGT GCGACACCTC TTGCTACAGC CCCTCCTGCT ATAGTACCTC CTGCTACAGT
    ACTTCCTGTT ACAGCACCTC TTGCTACAGC ACCTCCTGCT ATGACAGTAA CAATCGCTTC
    TCCAGCCATA CCCGCTTCTC CTCTGTTGAC AGTGCAAAGA TTTCTGAGAG CACGGTCTTT
    TCCTCACAGG ATGAGGAGGA GGAGAATAGT GCTTTTGAAT CGGTGCCAGA CTCAGTACAG
    AGCCCTGAGC TGGACAGGGA GCAAGTGGAT GGCTCCTCCC AGTGGCCAGA TGAACTAGTA
    GCTCATGGTG GGAATCCACA ACTAGCCGCA GAAAGTCTAG ACACCCCAAT AGCAGGTCCA
    AGCAGCAGAA GAGAAGGTGA TTGTCCTTTA CTCCATAATT CTCAGCCAGT CAGCCAACTT
    CCTTCTCTGA GGCCTGACCA TCATCACTAC CCAACTATCG ATGAGCCTCT TCCACCAACT
    GCAACATCCT TGAGAAACCT TACCCAGTTG GATAACCAGA GAGCCCTCCC TTTAAAATTA
    TGTGATGTTC ACAGAGAGAC TGTATATTGG GAAGCTCGGA TAGACAGCCA CGGGAGGGTG
    TTCTACGTTG ACCATGTCAA CCGAACTACC ACATGGCAGC GCCCCACAGC TGCAGCAACA
    CCAGATGGGA TCCACAGGTC TGGCTCTATC CAGCAAATGG AGCAGCTGAA CCGGCGATAT
    CAAAATATCC AGCGAACTAT TGCAACAGAG CGGACTGAAG ATGATGGTGT AGTAAACAAC
    AGAGTGGAGA GAATTCCCAC TGGAGGAGGA AGTGACTCTG AGGCAGAGCC TTCCCAACCA
    AGCTCCGAAA TTAGGAGGGA AGGTTCCCTT TCTCCTGTGA ATTCTCAAAA AATCACCTTA
    TTGCTGCAGT CTCCAGCTGT GAAGTTTATC ACCAATCCTG AGTTCTTCAC TGTGCTGCAT
    GCAAACTATA GTGCCTATCG AGTCTTCACT AACAGTACCT GCTTGAAGCA TATGATCCTG
    AAGATCCGTA GAGATGCTCG TAATTTTGAG CGCTATCAGC ACAACAGAGA CCTTGTAAAT
    TTTATCAACA TGTTTGCTGA CACTCGACTA GAGCTTCCTC GTGGCTGGGA AATAAAAACT
    GACCAGCAGG GCAAGTCTTT CTTCGTTGAC CACAACAGTC GTGCTACCAC ATTCATAGAT
    CCCAGGATCC CACTGCAGAA TGGTCGTCTC CCCAATCACT TGACTCACAG GCAACATCTT
    CAGCGGCTTC GTAGCTACAG TGCTGGAGAG GCTTCTGAAG TCTCTCGGAA CAGAGGAGTT
    ACTCTGTTGG CCAGACCAGG CAATAGTCTT GTAACAGCTA TTCGAAACCA GCATCATTAT
    GAGACATTAC CTCTGGCTTA CAATGACAAG ATTGTTGCAT TTCTACGCCA ACCTAACATT
    TTTGAGATGC TGCAAGAGCG TCAGCCCAGT TTGGCCAGAA ATCATGCACT CAGGGAGAAG
    ATCCATTATA TTCGGACAGA GGGTACACAT GGGCTTGAAA AGTTGTCCTG TGATGCAGAT
    TTAGTAATTC TGCTGAGCCT TTTTGAAGAA GATATCATGT CCTACATACC ACTCCAGGCC
    ACTTTCCATC CTGGTTACAG TTTCTCTCCT CGCTGCTCAC CCTGCTCATC TCCCCAAAAT
    TCTCCAGGAC TGCAGCGAGC AAGTGCAAGA GCACCCTCTC CATACAGGAG AGATTTTGAA
    GCCAAACTAC GCAATTTCTA TCGAAAACTT GAAGCCAAAG GATATGGACA AGGTCCAGGT
    AAAATTAAGT TAATTATACG ACGTGACCAC TTACTGGAAG GCACCTTCAA CCAGGTGATG
    GCATATTCAC GCAAGGAGCT CCAGCGAAAC AAACTCTACA TCACATTTGT TGGCGAGGAA
    GGGCTGGACT ACAGTGGCCC CTCTCGAGAA TTCTTTTTCC TTCTGTCTCA GGAGCTCTTC
    AATCCATATT ACGGGCTGTT TGAGTATTCA GCCAATGACA CTTACACTGT ACAGATTAGC
    CCCATGTCTG CCTTTGTTGA AAATCACCTG GAATGGTTCA GGTTCAGTGG TCGTATTCTT
    GGCCTTGCTC TCATTCATCA GTACCTTCTT GATGCTTTCT TCACAAGGCC GTTCTACAAA
    GCACTATTAA GGCTGCCATG TGATCTAAGT GACTTAGAGT ACCTGGATGA AGAATTCCAT
    CAGAGCTTGC AGTGGATGAA AGATAACAAC ATCACAGATA TCCTGGATCT CACCTTCACA
    GTGAATGAGG AGGTGTTTGG ACAGGTGACA GAGAGAGAGT TGAAATCTGG AGGGGCAAAT
    ACAGCAGTGA CAGAAAAGAA CAAAAAAGAG TACATTGAGA GAATGGTTAA ATGGCGAGTG
    GAACGAGGAG TTGTTCAACA AACAGAGGCC CTGGTCCGTG GCTTCTATGA AGTTGTAGAC
    TCAAGGCTTG TCTCTGTTTT TGATGCCAGA GAACTGGAGC TGGTTATAGC TGGCACTGCA
    GAAATAGATC TCAATGACTG GCGTAACAAC ACGGAATATC GTGGAGGTTA CCACGATGGA
    CACATTGTAA TACGGTGGTT CTGGGCAGCA GTGGAGAGGT TTAACAATGA ACAGAGACTC
    CGGTTGTTGC AGTTTGTTAC TGGAACATCA AGTGTGCCAT ATGAGGGCTT TGCAGCTCTC
    CGAGGAAGCA ATGGGCTTCG GCGTTTCTGT ATAGAGAAAT GGGGGAAAAT AACCTCCCTT
    CCGAGGGCAC ATACATGTTT CAACCGCTTG GATCTTCCAC CTTACCCCTC ATATTCTATG
    CTGTATGAAA AGCTGTTGAC AGCTGTTGAA GAAACTAGTA CCTTTGGACT TGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137101.1
    CDS171..5087
    Translation

    Target ORF information:

    RefSeq Version XM_015281615.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281615.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACTGTG CCCAGGCCGG CATTGTCCAG AATCTGTACC AAAACAGGTT CCTGGGCCTC 
    GCAGCCATGG CGTCTCCATC TAGGAACTCA CAAAATCGGC GCCGGTGCAA AGAGCCACTC
    AGATACAGCT ATAACCCTGA CCAGTTTCAT AACATGGACA TCAGGAACAA TTCCCATGAG
    ACAGTCACCA TTCCTAGGTC TACCAGTGAC ACGGACCTTG TCACTTCAGA CAGCCGATCT
    ACCCTGATGG TCAGCAGCTC CTACTATTCC ATTGGGCACT CACAGGACCT AGTGATATAC
    TGGGATATAA AGGAGGAAGT GGATGCAGGG GACTGGATTG GCATGTATCT CATAGATGAA
    GTCTTGTCTG AAAACTTTTT GGACTACAAG AACCGTGGAG TCAATGGCTC TCATCGTGGT
    CAGATTGTCT GGAAGATTGA TGCCAGTTCT TACTTTGTGG AGCCTGAAAC AAAGATATGC
    TTCAAATACT ATCACGGTGT GAGTGGGGCA CTACGAGCCA CAACTCCAAG CGTCACGGTG
    AAAAACAATG CAGCTCCTCA GATTTTTAAA AGCATCACCA ATGAAGATTC AATCCAGGGA
    CAGGGAAATC GGAGGCTGAT AAGTTTTTCA CTGTCAGATT TCCAAGCTTT TGGTCTGAAG
    AAAGGAATGT TTTTCAATCC AGATCCTTAT CTGAAGATTT CCATCCAGCC TGGGAAACAC
    AGCATCTTCC CAGCACTTCC TCATCATGGA CAGGAGAAAA GGTCTAAGAT CATGTGTAAT
    ACAGTTAACC CAATCTGGCA AGGAGAGCAA TTCAGCTTTG TCTCACTGCC CACGGATGTC
    CTGGAAATAG AAGTTAAAGA CAAATTTGCA AAGAGCCGAC CAATCATCAA GCGTTTCCTG
    GGAAAACTCT CTATGCCAGT TCAGCGTCTC TTGGAAAGAC ATGCCATAGG GGACAGGGTT
    GTAAGCTACA CTCTTGGTCG CAGGCTTCCT ACAGATCATG TCAGTGGCCA GCTGCAGTTC
    CGATTTGAGA TAACTTCTTC CATTCATCAA GATGATGAAG AGGTTACCAT TAGTGCAGAT
    CCTGAGCCAG CTGACCTCCA GGAAAGCCCT GTGAACAACC CCACAGAGGA AAGCCTCGGT
    GAGCCTCCAT GCATCAACAC AGTGACCTCA GAAAATACAG TTCCTGAAGA GCTAAATGGA
    TACAGTGTGA ACAATATTGA TAGCCCAGGG GCTGTCAAAC CACCCAGGGA AGTCAACCAG
    AACAACATAA ATGAAGAGCT AGCAGGAGAT GAGGAGGTTG ATGCCATGCC AGTTCCTGAA
    CCCTTGGAAT CCATCCGAGG AGAAGTGCTG CCTTCTTTGA ATGCGACAGA CCTCACAGAG
    CAGCTGGGTC TGCTGGCTTG CTTGTCTCCT TCTGAGACTG AAGTTAGGGA AAATAGCAAA
    GTCAATTCTG GTACTCAGGG AGATGATGGA GTATTGAACA GTACAGAAAC TTTAGATGTT
    AGTGAGGTGA GTGCAGATGT ATATGCTGAC AAAGACCTAG AGAAGAGTCA TGAAGAAGAA
    GGGGCTTCAG CCCGAGGGGA GGAAGACAGC AAGCTACAGC TGAGGACCAC AGCAAAGAGG
    AAAACAAGGC CATGCTCCCT GCCTGTGTCT GAAATTGAGA CCGTTATAGC TTCAGCTTGT
    GGTGAGCCAG AGACCCCTCG CACTCACTAT ATACGAATCC ACAACCTGCT CCACAGCCTG
    CCCTCAGCAC AGATGAGCAG TGATGGGGAA GAAGAGCAAG ATGCTGGCAA TGGTGGGGAG
    AATGATGAAG AGTCTACAGT CAAGGAAGTG TTGCAGAAGG AGACAGTCAG TGAAAGTGAG
    ACAATGGCAG CAGAGCCTCC TCCAGAAAAT GAGGCCGAAG AGAACTTCAG CCAGTGCACA
    GTGCCTCCTG GGACCTTAGA CGAGCAACTC TCTGACTTAA ATGATGGACT GGTGGATGGG
    GAAAACCCTA GGACAGGAAG CGAAAGCGAG TCAAGCTCCA GACCCAATGG TGACAACGAA
    TGTGAGGCGT GCGACACCTC TTGCTACAGC CCCTCCTGCT ATAGTACCTC CTGCTACAGT
    ACTTCCTGTT ACAGCACCTC TTGCTACAGC ACCTCCTGCT ATGACAGTAA CAATCGCTTC
    TCCAGCCATA CCCGCTTCTC CTCTGTTGAC AGTGCAAAGA TTTCTGAGAG CACGGTCTTT
    TCCTCACAGG ATGAGGAGGA GGAGAATAGT GCTTTTGAAT CGGTGCCAGA CTCAGTACAG
    AGCCCTGAGC TGGACAGGGA GCAAGTGGAT GGCTCCTCCC AGTGGCCAGA TGAACTAGTA
    GCTCATGGTG GGAATCCACA ACTAGCCGCA GAAAGTCTAG ACACCCCAAT AGCAGGTCCA
    AGCAGCAGAA GAGAAGGTGA TTGTCCTTTA CTCCATAATT CTCAGCCAGT CAGCCAACTT
    CCTTCTCTGA GGCCTGACCA TCATCACTAC CCAACTATCG ATGAGCCTCT TCCACCAACT
    GCAACATCCT TGAGAAACCT TACCCAGTTG GATAACCAGA GAGCCCTCCC TTTAAAATTA
    TGTGATGTTC ACAGAGAGAC TGTATATTGG GAAGCTCGGA TAGACAGCCA CGGGAGGGTG
    TTCTACGTTG ACCATGTCAA CCGAACTACC ACATGGCAGC GCCCCACAGC TGCAGCAACA
    CCAGATGGGA TCCACAGGTC TGGCTCTATC CAGCAAATGG AGCAGCTGAA CCGGCGATAT
    CAAAATATCC AGCGAACTAT TGCAACAGAG CGGACTGAAG ATGATGGTGT AGTAAACAAC
    AGAGTGGAGA GAATTCCCAC TGGAGGAGGA AGTGACTCTG AGGCAGAGCC TTCCCAACCA
    AGCTCCGAAA TTAGGAGGGA AGGTTCCCTT TCTCCTGTGA ATTCTCAAAA AATCACCTTA
    TTGCTGCAGT CTCCAGCTGT GAAGTTTATC ACCAATCCTG AGTTCTTCAC TGTGCTGCAT
    GCAAACTATA GTGCCTATCG AGTCTTCACT AACAGTACCT GCTTGAAGCA TATGATCCTG
    AAGATCCGTA GAGATGCTCG TAATTTTGAG CGCTATCAGC ACAACAGAGA CCTTGTAAAT
    TTTATCAACA TGTTTGCTGA CACTCGACTA GAGCTTCCTC GTGGCTGGGA AATAAAAACT
    GACCAGCAGG GCAAGTCTTT CTTCGTTGAC CACAACAGTC GTGCTACCAC ATTCATAGAT
    CCCAGGATCC CACTGCAGAA TGGTCGTCTC CCCAATCACT TGACTCACAG GCAACATCTT
    CAGCGGCTTC GTAGCTACAG TGCTGGAGAG GCTTCTGAAG TCTCTCGGAA CAGAGGAGTT
    ACTCTGTTGG CCAGACCAGG CAATAGTCTT GTAACAGCTA TTCGAAACCA GCATCATTAT
    GAGACATTAC CTCTGGCTTA CAATGACAAG ATTGTTGCAT TTCTACGCCA ACCTAACATT
    TTTGAGATGC TGCAAGAGCG TCAGCCCAGT TTGGCCAGAA ATCATGCACT CAGGGAGAAG
    ATCCATTATA TTCGGACAGA GGGTACACAT GGGCTTGAAA AGTTGTCCTG TGATGCAGAT
    TTAGTAATTC TGCTGAGCCT TTTTGAAGAA GATATCATGT CCTACATACC ACTCCAGGCC
    ACTTTCCATC CTGGTTACAG TTTCTCTCCT CGCTGCTCAC CCTGCTCATC TCCCCAAAAT
    TCTCCAGGAC TGCAGCGAGC AAGTGCAAGA GCACCCTCTC CATACAGGAG AGATTTTGAA
    GCCAAACTAC GCAATTTCTA TCGAAAACTT GAAGCCAAAG GATATGGACA AGGTCCAGGT
    AAAATTAAGT TAATTATACG ACGTGACCAC TTACTGGAAG GCACCTTCAA CCAGGTGATG
    GCATATTCAC GCAAGGAGCT CCAGCGAAAC AAACTCTACA TCACATTTGT TGGCGAGGAA
    GGGCTGGACT ACAGTGGCCC CTCTCGAGAA TTCTTTTTCC TTCTGTCTCA GGAGCTCTTC
    AATCCATATT ACGGGCTGTT TGAGTATTCA GCCAATGACA CTTACACTGT ACAGATTAGC
    CCCATGTCTG CCTTTGTTGA AAATCACCTG GAATGGTTCA GGTTCAGTGG TCGTATTCTT
    GGCCTTGCTC TCATTCATCA GTACCTTCTT GATGCTTTCT TCACAAGGCC GTTCTACAAA
    GCACTATTAA GGCTGCCATG TGATCTAAGT GACTTAGAGT ACCTGGATGA AGAATTCCAT
    CAGAGCTTGC AGTGGATGAA AGATAACAAC ATCACAGATA TCCTGGATCT CACCTTCACA
    GTGAATGAGG AGGTGTTTGG ACAGGTGACA GAGAGAGAGT TGAAATCTGG AGGGGCAAAT
    ACAGCAGTGA CAGAAAAGAA CAAAAAAGAG TACATTGAGA GAATGGTTAA ATGGCGAGTG
    GAACGAGGAG TTGTTCAACA AACAGAGGCC CTGGTCCGTG GCTTCTATGA AGTTGTAGAC
    TCAAGGCTTG TCTCTGTTTT TGATGCCAGA GAACTGGAGC TGGTTATAGC TGGCACTGCA
    GAAATAGATC TCAATGACTG GCGTAACAAC ACGGAATATC GTGGAGGTTA CCACGATGGA
    CACATTGTAA TACGGTGGTT CTGGGCAGCA GTGGAGAGGT TTAACAATGA ACAGAGACTC
    CGGTTGTTGC AGTTTGTTAC TGGAACATCA AGTGTGCCAT ATGAGGGCTT TGCAGCTCTC
    CGAGGAAGCA ATGGGCTTCG GCGTTTCTGT ATAGAGAAAT GGGGGAAAAT AACCTCCCTT
    CCGAGGGCAC ATACATGTTT CAACCGCTTG GATCTTCCAC CTTACCCCTC ATATTCTATG
    CTGTATGAAA AGCTGTTGAC AGCTGTTGAA GAAACTAGTA CCTTTGGACT TGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa31690
    Clone ID Related Accession (Same CDS sequence) XM_015281615.1 , XM_015281615.2
    Accession Version XM_015281615.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281615.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACTGTG CCCAGGCCGG CATTGTCCAG AATCTGTACC AAAACAGGTT CCTGGGCCTC 
    GCAGCCATGG CGTCTCCATC TAGGAACTCA CAAAATCGGC GCCGGTGCAA AGAGCCACTC
    AGATACAGCT ATAACCCTGA CCAGTTTCAT AACATGGACA TCAGGAACAA TTCCCATGAG
    ACAGTCACCA TTCCTAGGTC TACCAGTGAC ACGGACCTTG TCACTTCAGA CAGCCGATCT
    ACCCTGATGG TCAGCAGCTC CTACTATTCC ATTGGGCACT CACAGGACCT AGTGATATAC
    TGGGATATAA AGGAGGAAGT GGATGCAGGG GACTGGATTG GCATGTATCT CATAGATGAA
    GTCTTGTCTG AAAACTTTTT GGACTACAAG AACCGTGGAG TCAATGGCTC TCATCGTGGT
    CAGATTGTCT GGAAGATTGA TGCCAGTTCT TACTTTGTGG AGCCTGAAAC AAAGATATGC
    TTCAAATACT ATCACGGTGT GAGTGGGGCA CTACGAGCCA CAACTCCAAG CGTCACGGTG
    AAAAACAATG CAGCTCCTCA GATTTTTAAA AGCATCACCA ATGAAGATTC AATCCAGGGA
    CAGGGAAATC GGAGGCTGAT AAGTTTTTCA CTGTCAGATT TCCAAGCTTT TGGTCTGAAG
    AAAGGAATGT TTTTCAATCC AGATCCTTAT CTGAAGATTT CCATCCAGCC TGGGAAACAC
    AGCATCTTCC CAGCACTTCC TCATCATGGA CAGGAGAAAA GGTCTAAGAT CATGTGTAAT
    ACAGTTAACC CAATCTGGCA AGGAGAGCAA TTCAGCTTTG TCTCACTGCC CACGGATGTC
    CTGGAAATAG AAGTTAAAGA CAAATTTGCA AAGAGCCGAC CAATCATCAA GCGTTTCCTG
    GGAAAACTCT CTATGCCAGT TCAGCGTCTC TTGGAAAGAC ATGCCATAGG GGACAGGGTT
    GTAAGCTACA CTCTTGGTCG CAGGCTTCCT ACAGATCATG TCAGTGGCCA GCTGCAGTTC
    CGATTTGAGA TAACTTCTTC CATTCATCAA GATGATGAAG AGGTTACCAT TAGTGCAGAT
    CCTGAGCCAG CTGACCTCCA GGAAAGCCCT GTGAACAACC CCACAGAGGA AAGCCTCGGT
    GAGCCTCCAT GCATCAACAC AGTGACCTCA GAAAATACAG TTCCTGAAGA GCTAAATGGA
    TACAGTGTGA ACAATATTGA TAGCCCAGGG GCTGTCAAAC CACCCAGGGA AGTCAACCAG
    AACAACATAA ATGAAGAGCT AGCAGGAGAT GAGGAGGTTG ATGCCATGCC AGTTCCTGAA
    CCCTTGGAAT CCATCCGAGG AGAAGTGCTG CCTTCTTTGA ATGCGACAGA CCTCACAGAG
    CAGCTGGGTC TGCTGGCTTG CTTGTCTCCT TCTGAGACTG AAGTTAGGGA AAATAGCAAA
    GTCAATTCTG GTACTCAGGG AGATGATGGA GTATTGAACA GTACAGAAAC TTTAGATGTT
    AGTGAGGTGA GTGCAGATGT ATATGCTGAC AAAGACCTAG AGAAGAGTCA TGAAGAAGAA
    GGGGCTTCAG CCCGAGGGGA GGAAGACAGC AAGCTACAGC TGAGGACCAC AGCAAAGAGG
    AAAACAAGGC CATGCTCCCT GCCTGTGTCT GAAATTGAGA CCGTTATAGC TTCAGCTTGT
    GGTGAGCCAG AGACCCCTCG CACTCACTAT ATACGAATCC ACAACCTGCT CCACAGCCTG
    CCCTCAGCAC AGATGAGCAG TGATGGGGAA GAAGAGCAAG ATGCTGGCAA TGGTGGGGAG
    AATGATGAAG AGTCTACAGT CAAGGAAGTG TTGCAGAAGG AGACAGTCAG TGAAAGTGAG
    ACAATGGCAG CAGAGCCTCC TCCAGAAAAT GAGGCCGAAG AGAACTTCAG CCAGTGCACA
    GTGCCTCCTG GGACCTTAGA CGAGCAACTC TCTGACTTAA ATGATGGACT GGTGGATGGG
    GAAAACCCTA GGACAGGAAG CGAAAGCGAG TCAAGCTCCA GACCCAATGG TGACAACGAA
    TGTGAGGCGT GCGACACCTC TTGCTACAGC CCCTCCTGCT ATAGTACCTC CTGCTACAGT
    ACTTCCTGTT ACAGCACCTC TTGCTACAGC ACCTCCTGCT ATGACAGTAA CAATCGCTTC
    TCCAGCCATA CCCGCTTCTC CTCTGTTGAC AGTGCAAAGA TTTCTGAGAG CACGGTCTTT
    TCCTCACAGG ATGAGGAGGA GGAGAATAGT GCTTTTGAAT CGGTGCCAGA CTCAGTACAG
    AGCCCTGAGC TGGACAGGGA GCAAGTGGAT GGCTCCTCCC AGTGGCCAGA TGAACTAGTA
    GCTCATGGTG GGAATCCACA ACTAGCCGCA GAAAGTCTAG ACACCCCAAT AGCAGGTCCA
    AGCAGCAGAA GAGAAGGTGA TTGTCCTTTA CTCCATAATT CTCAGCCAGT CAGCCAACTT
    CCTTCTCTGA GGCCTGACCA TCATCACTAC CCAACTATCG ATGAGCCTCT TCCACCAACT
    GCAACATCCT TGAGAAACCT TACCCAGTTG GATAACCAGA GAGCCCTCCC TTTAAAATTA
    TGTGATGTTC ACAGAGAGAC TGTATATTGG GAAGCTCGGA TAGACAGCCA CGGGAGGGTG
    TTCTACGTTG ACCATGTCAA CCGAACTACC ACATGGCAGC GCCCCACAGC TGCAGCAACA
    CCAGATGGGA TCCACAGGTC TGGCTCTATC CAGCAAATGG AGCAGCTGAA CCGGCGATAT
    CAAAATATCC AGCGAACTAT TGCAACAGAG CGGACTGAAG ATGATGGTGT AGTAAACAAC
    AGAGTGGAGA GAATTCCCAC TGGAGGAGGA AGTGACTCTG AGGCAGAGCC TTCCCAACCA
    AGCTCCGAAA TTAGGAGGGA AGGTTCCCTT TCTCCTGTGA ATTCTCAAAA AATCACCTTA
    TTGCTGCAGT CTCCAGCTGT GAAGTTTATC ACCAATCCTG AGTTCTTCAC TGTGCTGCAT
    GCAAACTATA GTGCCTATCG AGTCTTCACT AACAGTACCT GCTTGAAGCA TATGATCCTG
    AAGATCCGTA GAGATGCTCG TAATTTTGAG CGCTATCAGC ACAACAGAGA CCTTGTAAAT
    TTTATCAACA TGTTTGCTGA CACTCGACTA GAGCTTCCTC GTGGCTGGGA AATAAAAACT
    GACCAGCAGG GCAAGTCTTT CTTCGTTGAC CACAACAGTC GTGCTACCAC ATTCATAGAT
    CCCAGGATCC CACTGCAGAA TGGTCGTCTC CCCAATCACT TGACTCACAG GCAACATCTT
    CAGCGGCTTC GTAGCTACAG TGCTGGAGAG GCTTCTGAAG TCTCTCGGAA CAGAGGAGTT
    ACTCTGTTGG CCAGACCAGG CAATAGTCTT GTAACAGCTA TTCGAAACCA GCATCATTAT
    GAGACATTAC CTCTGGCTTA CAATGACAAG ATTGTTGCAT TTCTACGCCA ACCTAACATT
    TTTGAGATGC TGCAAGAGCG TCAGCCCAGT TTGGCCAGAA ATCATGCACT CAGGGAGAAG
    ATCCATTATA TTCGGACAGA GGGTACACAT GGGCTTGAAA AGTTGTCCTG TGATGCAGAT
    TTAGTAATTC TGCTGAGCCT TTTTGAAGAA GATATCATGT CCTACATACC ACTCCAGGCC
    ACTTTCCATC CTGGTTACAG TTTCTCTCCT CGCTGCTCAC CCTGCTCATC TCCCCAAAAT
    TCTCCAGGAC TGCAGCGAGC AAGTGCAAGA GCACCCTCTC CATACAGGAG AGATTTTGAA
    GCCAAACTAC GCAATTTCTA TCGAAAACTT GAAGCCAAAG GATATGGACA AGGTCCAGGT
    AAAATTAAGT TAATTATACG ACGTGACCAC TTACTGGAAG GCACCTTCAA CCAGGTGATG
    GCATATTCAC GCAAGGAGCT CCAGCGAAAC AAACTCTACA TCACATTTGT TGGCGAGGAA
    GGGCTGGACT ACAGTGGCCC CTCTCGAGAA TTCTTTTTCC TTCTGTCTCA GGAGCTCTTC
    AATCCATATT ACGGGCTGTT TGAGTATTCA GCCAATGACA CTTACACTGT ACAGATTAGC
    CCCATGTCTG CCTTTGTTGA AAATCACCTG GAATGGTTCA GGTTCAGTGG TCGTATTCTT
    GGCCTTGCTC TCATTCATCA GTACCTTCTT GATGCTTTCT TCACAAGGCC GTTCTACAAA
    GCACTATTAA GGCTGCCATG TGATCTAAGT GACTTAGAGT ACCTGGATGA AGAATTCCAT
    CAGAGCTTGC AGTGGATGAA AGATAACAAC ATCACAGATA TCCTGGATCT CACCTTCACA
    GTGAATGAGG AGGTGTTTGG ACAGGTGACA GAGAGAGAGT TGAAATCTGG AGGGGCAAAT
    ACAGCAGTGA CAGAAAAGAA CAAAAAAGAG TACATTGAGA GAATGGTTAA ATGGCGAGTG
    GAACGAGGAG TTGTTCAACA AACAGAGGCC CTGGTCCGTG GCTTCTATGA AGTTGTAGAC
    TCAAGGCTTG TCTCTGTTTT TGATGCCAGA GAACTGGAGC TGGTTATAGC TGGCACTGCA
    GAAATAGATC TCAATGACTG GCGTAACAAC ACGGAATATC GTGGAGGTTA CCACGATGGA
    CACATTGTAA TACGGTGGTT CTGGGCAGCA GTGGAGAGGT TTAACAATGA ACAGAGACTC
    CGGTTGTTGC AGTTTGTTAC TGGAACATCA AGTGTGCCAT ATGAGGGCTT TGCAGCTCTC
    CGAGGAAGCA ATGGGCTTCG GCGTTTCTGT ATAGAGAAAT GGGGGAAAAT AACCTCCCTT
    CCGAGGGCAC ATACATGTTT CAACCGCTTG GATCTTCCAC CTTACCCCTC ATATTCTATG
    CTGTATGAAA AGCTGTTGAC AGCTGTTGAA GAAACTAGTA CCTTTGGACT TGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137101.1
    CDS165..5081
    Translation

    Target ORF information:

    RefSeq Version XM_015281615.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281615.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAACTGTG CCCAGGCCGG CATTGTCCAG AATCTGTACC AAAACAGGTT CCTGGGCCTC 
    GCAGCCATGG CGTCTCCATC TAGGAACTCA CAAAATCGGC GCCGGTGCAA AGAGCCACTC
    AGATACAGCT ATAACCCTGA CCAGTTTCAT AACATGGACA TCAGGAACAA TTCCCATGAG
    ACAGTCACCA TTCCTAGGTC TACCAGTGAC ACGGACCTTG TCACTTCAGA CAGCCGATCT
    ACCCTGATGG TCAGCAGCTC CTACTATTCC ATTGGGCACT CACAGGACCT AGTGATATAC
    TGGGATATAA AGGAGGAAGT GGATGCAGGG GACTGGATTG GCATGTATCT CATAGATGAA
    GTCTTGTCTG AAAACTTTTT GGACTACAAG AACCGTGGAG TCAATGGCTC TCATCGTGGT
    CAGATTGTCT GGAAGATTGA TGCCAGTTCT TACTTTGTGG AGCCTGAAAC AAAGATATGC
    TTCAAATACT ATCACGGTGT GAGTGGGGCA CTACGAGCCA CAACTCCAAG CGTCACGGTG
    AAAAACAATG CAGCTCCTCA GATTTTTAAA AGCATCACCA ATGAAGATTC AATCCAGGGA
    CAGGGAAATC GGAGGCTGAT AAGTTTTTCA CTGTCAGATT TCCAAGCTTT TGGTCTGAAG
    AAAGGAATGT TTTTCAATCC AGATCCTTAT CTGAAGATTT CCATCCAGCC TGGGAAACAC
    AGCATCTTCC CAGCACTTCC TCATCATGGA CAGGAGAAAA GGTCTAAGAT CATGTGTAAT
    ACAGTTAACC CAATCTGGCA AGGAGAGCAA TTCAGCTTTG TCTCACTGCC CACGGATGTC
    CTGGAAATAG AAGTTAAAGA CAAATTTGCA AAGAGCCGAC CAATCATCAA GCGTTTCCTG
    GGAAAACTCT CTATGCCAGT TCAGCGTCTC TTGGAAAGAC ATGCCATAGG GGACAGGGTT
    GTAAGCTACA CTCTTGGTCG CAGGCTTCCT ACAGATCATG TCAGTGGCCA GCTGCAGTTC
    CGATTTGAGA TAACTTCTTC CATTCATCAA GATGATGAAG AGGTTACCAT TAGTGCAGAT
    CCTGAGCCAG CTGACCTCCA GGAAAGCCCT GTGAACAACC CCACAGAGGA AAGCCTCGGT
    GAGCCTCCAT GCATCAACAC AGTGACCTCA GAAAATACAG TTCCTGAAGA GCTAAATGGA
    TACAGTGTGA ACAATATTGA TAGCCCAGGG GCTGTCAAAC CACCCAGGGA AGTCAACCAG
    AACAACATAA ATGAAGAGCT AGCAGGAGAT GAGGAGGTTG ATGCCATGCC AGTTCCTGAA
    CCCTTGGAAT CCATCCGAGG AGAAGTGCTG CCTTCTTTGA ATGCGACAGA CCTCACAGAG
    CAGCTGGGTC TGCTGGCTTG CTTGTCTCCT TCTGAGACTG AAGTTAGGGA AAATAGCAAA
    GTCAATTCTG GTACTCAGGG AGATGATGGA GTATTGAACA GTACAGAAAC TTTAGATGTT
    AGTGAGGTGA GTGCAGATGT ATATGCTGAC AAAGACCTAG AGAAGAGTCA TGAAGAAGAA
    GGGGCTTCAG CCCGAGGGGA GGAAGACAGC AAGCTACAGC TGAGGACCAC AGCAAAGAGG
    AAAACAAGGC CATGCTCCCT GCCTGTGTCT GAAATTGAGA CCGTTATAGC TTCAGCTTGT
    GGTGAGCCAG AGACCCCTCG CACTCACTAT ATACGAATCC ACAACCTGCT CCACAGCCTG
    CCCTCAGCAC AGATGAGCAG TGATGGGGAA GAAGAGCAAG ATGCTGGCAA TGGTGGGGAG
    AATGATGAAG AGTCTACAGT CAAGGAAGTG TTGCAGAAGG AGACAGTCAG TGAAAGTGAG
    ACAATGGCAG CAGAGCCTCC TCCAGAAAAT GAGGCCGAAG AGAACTTCAG CCAGTGCACA
    GTGCCTCCTG GGACCTTAGA CGAGCAACTC TCTGACTTAA ATGATGGACT GGTGGATGGG
    GAAAACCCTA GGACAGGAAG CGAAAGCGAG TCAAGCTCCA GACCCAATGG TGACAACGAA
    TGTGAGGCGT GCGACACCTC TTGCTACAGC CCCTCCTGCT ATAGTACCTC CTGCTACAGT
    ACTTCCTGTT ACAGCACCTC TTGCTACAGC ACCTCCTGCT ATGACAGTAA CAATCGCTTC
    TCCAGCCATA CCCGCTTCTC CTCTGTTGAC AGTGCAAAGA TTTCTGAGAG CACGGTCTTT
    TCCTCACAGG ATGAGGAGGA GGAGAATAGT GCTTTTGAAT CGGTGCCAGA CTCAGTACAG
    AGCCCTGAGC TGGACAGGGA GCAAGTGGAT GGCTCCTCCC AGTGGCCAGA TGAACTAGTA
    GCTCATGGTG GGAATCCACA ACTAGCCGCA GAAAGTCTAG ACACCCCAAT AGCAGGTCCA
    AGCAGCAGAA GAGAAGGTGA TTGTCCTTTA CTCCATAATT CTCAGCCAGT CAGCCAACTT
    CCTTCTCTGA GGCCTGACCA TCATCACTAC CCAACTATCG ATGAGCCTCT TCCACCAACT
    GCAACATCCT TGAGAAACCT TACCCAGTTG GATAACCAGA GAGCCCTCCC TTTAAAATTA
    TGTGATGTTC ACAGAGAGAC TGTATATTGG GAAGCTCGGA TAGACAGCCA CGGGAGGGTG
    TTCTACGTTG ACCATGTCAA CCGAACTACC ACATGGCAGC GCCCCACAGC TGCAGCAACA
    CCAGATGGGA TCCACAGGTC TGGCTCTATC CAGCAAATGG AGCAGCTGAA CCGGCGATAT
    CAAAATATCC AGCGAACTAT TGCAACAGAG CGGACTGAAG ATGATGGTGT AGTAAACAAC
    AGAGTGGAGA GAATTCCCAC TGGAGGAGGA AGTGACTCTG AGGCAGAGCC TTCCCAACCA
    AGCTCCGAAA TTAGGAGGGA AGGTTCCCTT TCTCCTGTGA ATTCTCAAAA AATCACCTTA
    TTGCTGCAGT CTCCAGCTGT GAAGTTTATC ACCAATCCTG AGTTCTTCAC TGTGCTGCAT
    GCAAACTATA GTGCCTATCG AGTCTTCACT AACAGTACCT GCTTGAAGCA TATGATCCTG
    AAGATCCGTA GAGATGCTCG TAATTTTGAG CGCTATCAGC ACAACAGAGA CCTTGTAAAT
    TTTATCAACA TGTTTGCTGA CACTCGACTA GAGCTTCCTC GTGGCTGGGA AATAAAAACT
    GACCAGCAGG GCAAGTCTTT CTTCGTTGAC CACAACAGTC GTGCTACCAC ATTCATAGAT
    CCCAGGATCC CACTGCAGAA TGGTCGTCTC CCCAATCACT TGACTCACAG GCAACATCTT
    CAGCGGCTTC GTAGCTACAG TGCTGGAGAG GCTTCTGAAG TCTCTCGGAA CAGAGGAGTT
    ACTCTGTTGG CCAGACCAGG CAATAGTCTT GTAACAGCTA TTCGAAACCA GCATCATTAT
    GAGACATTAC CTCTGGCTTA CAATGACAAG ATTGTTGCAT TTCTACGCCA ACCTAACATT
    TTTGAGATGC TGCAAGAGCG TCAGCCCAGT TTGGCCAGAA ATCATGCACT CAGGGAGAAG
    ATCCATTATA TTCGGACAGA GGGTACACAT GGGCTTGAAA AGTTGTCCTG TGATGCAGAT
    TTAGTAATTC TGCTGAGCCT TTTTGAAGAA GATATCATGT CCTACATACC ACTCCAGGCC
    ACTTTCCATC CTGGTTACAG TTTCTCTCCT CGCTGCTCAC CCTGCTCATC TCCCCAAAAT
    TCTCCAGGAC TGCAGCGAGC AAGTGCAAGA GCACCCTCTC CATACAGGAG AGATTTTGAA
    GCCAAACTAC GCAATTTCTA TCGAAAACTT GAAGCCAAAG GATATGGACA AGGTCCAGGT
    AAAATTAAGT TAATTATACG ACGTGACCAC TTACTGGAAG GCACCTTCAA CCAGGTGATG
    GCATATTCAC GCAAGGAGCT CCAGCGAAAC AAACTCTACA TCACATTTGT TGGCGAGGAA
    GGGCTGGACT ACAGTGGCCC CTCTCGAGAA TTCTTTTTCC TTCTGTCTCA GGAGCTCTTC
    AATCCATATT ACGGGCTGTT TGAGTATTCA GCCAATGACA CTTACACTGT ACAGATTAGC
    CCCATGTCTG CCTTTGTTGA AAATCACCTG GAATGGTTCA GGTTCAGTGG TCGTATTCTT
    GGCCTTGCTC TCATTCATCA GTACCTTCTT GATGCTTTCT TCACAAGGCC GTTCTACAAA
    GCACTATTAA GGCTGCCATG TGATCTAAGT GACTTAGAGT ACCTGGATGA AGAATTCCAT
    CAGAGCTTGC AGTGGATGAA AGATAACAAC ATCACAGATA TCCTGGATCT CACCTTCACA
    GTGAATGAGG AGGTGTTTGG ACAGGTGACA GAGAGAGAGT TGAAATCTGG AGGGGCAAAT
    ACAGCAGTGA CAGAAAAGAA CAAAAAAGAG TACATTGAGA GAATGGTTAA ATGGCGAGTG
    GAACGAGGAG TTGTTCAACA AACAGAGGCC CTGGTCCGTG GCTTCTATGA AGTTGTAGAC
    TCAAGGCTTG TCTCTGTTTT TGATGCCAGA GAACTGGAGC TGGTTATAGC TGGCACTGCA
    GAAATAGATC TCAATGACTG GCGTAACAAC ACGGAATATC GTGGAGGTTA CCACGATGGA
    CACATTGTAA TACGGTGGTT CTGGGCAGCA GTGGAGAGGT TTAACAATGA ACAGAGACTC
    CGGTTGTTGC AGTTTGTTAC TGGAACATCA AGTGTGCCAT ATGAGGGCTT TGCAGCTCTC
    CGAGGAAGCA ATGGGCTTCG GCGTTTCTGT ATAGAGAAAT GGGGGAAAAT AACCTCCCTT
    CCGAGGGCAC ATACATGTTT CAACCGCTTG GATCTTCCAC CTTACCCCTC ATATTCTATG
    CTGTATGAAA AGCTGTTGAC AGCTGTTGAA GAAACTAGTA CCTTTGGACT TGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa31691
    Clone ID Related Accession (Same CDS sequence) XM_015281616.2 , XM_015281616.1
    Accession Version XM_015281616.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281616.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCTGCTCC ACCTGTGTAG TGTTAAGAAT CTGTACCAAA ACAGGTTCCT GGGCCTCGCA 
    GCCATGGCGT CTCCATCTAG GAACTCACAA AATCGGCGCC GGTGCAAAGA GCCACTCAGA
    TACAGCTATA ACCCTGACCA GTTTCATAAC ATGGACATCA GGAACAATTC CCATGAGACA
    GTCACCATTC CTAGGTCTAC CAGTGACACG GACCTTGTCA CTTCAGACAG CCGATCTACC
    CTGATGGTCA GCAGCTCCTA CTATTCCATT GGGCACTCAC AGGACCTAGT GATATACTGG
    GATATAAAGG AGGAAGTGGA TGCAGGGGAC TGGATTGGCA TGTATCTCAT AGATGAAGTC
    TTGTCTGAAA ACTTTTTGGA CTACAAGAAC CGTGGAGTCA ATGGCTCTCA TCGTGGTCAG
    ATTGTCTGGA AGATTGATGC CAGTTCTTAC TTTGTGGAGC CTGAAACAAA GATATGCTTC
    AAATACTATC ACGGTGTGAG TGGGGCACTA CGAGCCACAA CTCCAAGCGT CACGGTGAAA
    AACAATGCAG CTCCTCAGAT TTTTAAAAGC ATCACCAATG AAGATTCAAT CCAGGGACAG
    GGAAATCGGA GGCTGATAAG TTTTTCACTG TCAGATTTCC AAGCTTTTGG TCTGAAGAAA
    GGAATGTTTT TCAATCCAGA TCCTTATCTG AAGATTTCCA TCCAGCCTGG GAAACACAGC
    ATCTTCCCAG CACTTCCTCA TCATGGACAG GAGAAAAGGT CTAAGATCAT GTGTAATACA
    GTTAACCCAA TCTGGCAAGG AGAGCAATTC AGCTTTGTCT CACTGCCCAC GGATGTCCTG
    GAAATAGAAG TTAAAGACAA ATTTGCAAAG AGCCGACCAA TCATCAAGCG TTTCCTGGGA
    AAACTCTCTA TGCCAGTTCA GCGTCTCTTG GAAAGACATG CCATAGGGGA CAGGGTTGTA
    AGCTACACTC TTGGTCGCAG GCTTCCTACA GATCATGTCA GTGGCCAGCT GCAGTTCCGA
    TTTGAGATAA CTTCTTCCAT TCATCAAGAT GATGAAGAGG TTACCATTAG TGCAGATCCT
    GAGCCAGCTG ACCTCCAGGA AAGCCCTGTG AACAACCCCA CAGAGGAAAG CCTCGGTGAG
    CCTCCATGCA TCAACACAGT GACCTCAGAA AATACAGTTC CTGAAGAGCT AAATGGATAC
    AGTGTGAACA ATATTGATAG CCCAGGGGCT GTCAAACCAC CCAGGGAAGT CAACCAGAAC
    AACATAAATG AAGAGCTAGC AGGAGATGAG GAGGTTGATG CCATGCCAGT TCCTGAACCC
    TTGGAATCCA TCCGAGGAGA AGTGCTGCCT TCTTTGAATG CGACAGACCT CACAGAGCAG
    CTGGGTCTGC TGGCTTGCTT GTCTCCTTCT GAGACTGAAG TTAGGGAAAA TAGCAAAGTC
    AATTCTGGTA CTCAGGGAGA TGATGGAGTA TTGAACAGTA CAGAAACTTT AGATGTTAGT
    GAGGTGAGTG CAGATGTATA TGCTGACAAA GACCTAGAGA AGAGTCATGA AGAAGAAGGG
    GCTTCAGCCC GAGGGGAGGA AGACAGCAAG CTACAGCTGA GGACCACAGC AAAGAGGAAA
    ACAAGGCCAT GCTCCCTGCC TGTGTCTGAA ATTGAGACCG TTATAGCTTC AGCTTGTGGT
    GAGCCAGAGA CCCCTCGCAC TCACTATATA CGAATCCACA ACCTGCTCCA CAGCCTGCCC
    TCAGCACAGA TGAGCAGTGA TGGGGAAGAA GAGCAAGATG CTGGCAATGG TGGGGAGAAT
    GATGAAGAGT CTACAGTCAA GGAAGTGTTG CAGAAGGAGA CAGTCAGTGA AAGTGAGACA
    ATGGCAGCAG AGCCTCCTCC AGAAAATGAG GCCGAAGAGA ACTTCAGCCA GTGCACAGTG
    CCTCCTGGGA CCTTAGACGA GCAACTCTCT GACTTAAATG ATGGACTGGT GGATGGGGAA
    AACCCTAGGA CAGGAAGCGA AAGCGAGTCA AGCTCCAGAC CCAATGGTGA CAACGAATGT
    GAGGCGTGCG ACACCTCTTG CTACAGCCCC TCCTGCTATA GTACCTCCTG CTACAGTACT
    TCCTGTTACA GCACCTCTTG CTACAGCACC TCCTGCTATG ACAGTAACAA TCGCTTCTCC
    AGCCATACCC GCTTCTCCTC TGTTGACAGT GCAAAGATTT CTGAGAGCAC GGTCTTTTCC
    TCACAGGATG AGGAGGAGGA GAATAGTGCT TTTGAATCGG TGCCAGACTC AGTACAGAGC
    CCTGAGCTGG ACAGGGAGCA AGTGGATGGC TCCTCCCAGT GGCCAGATGA ACTAGTAGCT
    CATGGTGGGA ATCCACAACT AGCCGCAGAA AGTCTAGACA CCCCAATAGC AGGTCCAAGC
    AGCAGAAGAG AAGGTGATTG TCCTTTACTC CATAATTCTC AGCCAGTCAG CCAACTTCCT
    TCTCTGAGGC CTGACCATCA TCACTACCCA ACTATCGATG AGCCTCTTCC ACCAACTGCA
    ACATCCTTGA GAAACCTTAC CCAGTTGGAT AACCAGAGAG CCCTCCCTTT AAAATTATGT
    GATGTTCACA GAGAGACTGT ATATTGGGAA GCTCGGATAG ACAGCCACGG GAGGGTGTTC
    TACGTTGACC ATGTCAACCG AACTACCACA TGGCAGCGCC CCACAGCTGC AGCAACACCA
    GATGGGATCC ACAGGTCTGG CTCTATCCAG CAAATGGAGC AGCTGAACCG GCGATATCAA
    AATATCCAGC GAACTATTGC AACAGAGCGG ACTGAAGATG ATGGTGTAGT AAACAACAGA
    GTGGAGAGAA TTCCCACTGG AGGAGGAAGT GACTCTGAGG CAGAGCCTTC CCAACCAAGC
    TCCGAAATTA GGAGGGAAGG TTCCCTTTCT CCTGTGAATT CTCAAAAAAT CACCTTATTG
    CTGCAGTCTC CAGCTGTGAA GTTTATCACC AATCCTGAGT TCTTCACTGT GCTGCATGCA
    AACTATAGTG CCTATCGAGT CTTCACTAAC AGTACCTGCT TGAAGCATAT GATCCTGAAG
    ATCCGTAGAG ATGCTCGTAA TTTTGAGCGC TATCAGCACA ACAGAGACCT TGTAAATTTT
    ATCAACATGT TTGCTGACAC TCGACTAGAG CTTCCTCGTG GCTGGGAAAT AAAAACTGAC
    CAGCAGGGCA AGTCTTTCTT CGTTGACCAC AACAGTCGTG CTACCACATT CATAGATCCC
    AGGATCCCAC TGCAGAATGG TCGTCTCCCC AATCACTTGA CTCACAGGCA ACATCTTCAG
    CGGCTTCGTA GCTACAGTGC TGGAGAGGCT TCTGAAGTCT CTCGGAACAG AGGAGTTACT
    CTGTTGGCCA GACCAGGCAA TAGTCTTGTA ACAGCTATTC GAAACCAGCA TCATTATGAG
    ACATTACCTC TGGCTTACAA TGACAAGATT GTTGCATTTC TACGCCAACC TAACATTTTT
    GAGATGCTGC AAGAGCGTCA GCCCAGTTTG GCCAGAAATC ATGCACTCAG GGAGAAGATC
    CATTATATTC GGACAGAGGG TACACATGGG CTTGAAAAGT TGTCCTGTGA TGCAGATTTA
    GTAATTCTGC TGAGCCTTTT TGAAGAAGAT ATCATGTCCT ACATACCACT CCAGGCCACT
    TTCCATCCTG GTTACAGTTT CTCTCCTCGC TGCTCACCCT GCTCATCTCC CCAAAATTCT
    CCAGGACTGC AGCGAGCAAG TGCAAGAGCA CCCTCTCCAT ACAGGAGAGA TTTTGAAGCC
    AAACTACGCA ATTTCTATCG AAAACTTGAA GCCAAAGGAT ATGGACAAGG TCCAGGTAAA
    ATTAAGTTAA TTATACGACG TGACCACTTA CTGGAAGGCA CCTTCAACCA GGTGATGGCA
    TATTCACGCA AGGAGCTCCA GCGAAACAAA CTCTACATCA CATTTGTTGG CGAGGAAGGG
    CTGGACTACA GTGGCCCCTC TCGAGAATTC TTTTTCCTTC TGTCTCAGGA GCTCTTCAAT
    CCATATTACG GGCTGTTTGA GTATTCAGCC AATGACACTT ACACTGTACA GATTAGCCCC
    ATGTCTGCCT TTGTTGAAAA TCACCTGGAA TGGTTCAGGT TCAGTGGTCG TATTCTTGGC
    CTTGCTCTCA TTCATCAGTA CCTTCTTGAT GCTTTCTTCA CAAGGCCGTT CTACAAAGCA
    CTATTAAGGC TGCCATGTGA TCTAAGTGAC TTAGAGTACC TGGATGAAGA ATTCCATCAG
    AGCTTGCAGT GGATGAAAGA TAACAACATC ACAGATATCC TGGATCTCAC CTTCACAGTG
    AATGAGGAGG TGTTTGGACA GGTGACAGAG AGAGAGTTGA AATCTGGAGG GGCAAATACA
    GCAGTGACAG AAAAGAACAA AAAAGAGTAC ATTGAGAGAA TGGTTAAATG GCGAGTGGAA
    CGAGGAGTTG TTCAACAAAC AGAGGCCCTG GTCCGTGGCT TCTATGAAGT TGTAGACTCA
    AGGCTTGTCT CTGTTTTTGA TGCCAGAGAA CTGGAGCTGG TTATAGCTGG CACTGCAGAA
    ATAGATCTCA ATGACTGGCG TAACAACACG GAATATCGTG GAGGTTACCA CGATGGACAC
    ATTGTAATAC GGTGGTTCTG GGCAGCAGTG GAGAGGTTTA ACAATGAACA GAGACTCCGG
    TTGTTGCAGT TTGTTACTGG AACATCAAGT GTGCCATATG AGGGCTTTGC AGCTCTCCGA
    GGAAGCAATG GGCTTCGGCG TTTCTGTATA GAGAAATGGG GGAAAATAAC CTCCCTTCCG
    AGGGCACATA CATGTTTCAA CCGCTTGGAT CTTCCACCTT ACCCCTCATA TTCTATGCTG
    TATGAAAAGC TGTTGACAGC TGTTGAAGAA ACTAGTACCT TTGGACTTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137102.1
    CDS224..5137
    Translation

    Target ORF information:

    RefSeq Version XM_015281616.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281616.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCTGCTCC ACCTGTGTAG TGTTAAGAAT CTGTACCAAA ACAGGTTCCT GGGCCTCGCA 
    GCCATGGCGT CTCCATCTAG GAACTCACAA AATCGGCGCC GGTGCAAAGA GCCACTCAGA
    TACAGCTATA ACCCTGACCA GTTTCATAAC ATGGACATCA GGAACAATTC CCATGAGACA
    GTCACCATTC CTAGGTCTAC CAGTGACACG GACCTTGTCA CTTCAGACAG CCGATCTACC
    CTGATGGTCA GCAGCTCCTA CTATTCCATT GGGCACTCAC AGGACCTAGT GATATACTGG
    GATATAAAGG AGGAAGTGGA TGCAGGGGAC TGGATTGGCA TGTATCTCAT AGATGAAGTC
    TTGTCTGAAA ACTTTTTGGA CTACAAGAAC CGTGGAGTCA ATGGCTCTCA TCGTGGTCAG
    ATTGTCTGGA AGATTGATGC CAGTTCTTAC TTTGTGGAGC CTGAAACAAA GATATGCTTC
    AAATACTATC ACGGTGTGAG TGGGGCACTA CGAGCCACAA CTCCAAGCGT CACGGTGAAA
    AACAATGCAG CTCCTCAGAT TTTTAAAAGC ATCACCAATG AAGATTCAAT CCAGGGACAG
    GGAAATCGGA GGCTGATAAG TTTTTCACTG TCAGATTTCC AAGCTTTTGG TCTGAAGAAA
    GGAATGTTTT TCAATCCAGA TCCTTATCTG AAGATTTCCA TCCAGCCTGG GAAACACAGC
    ATCTTCCCAG CACTTCCTCA TCATGGACAG GAGAAAAGGT CTAAGATCAT GTGTAATACA
    GTTAACCCAA TCTGGCAAGG AGAGCAATTC AGCTTTGTCT CACTGCCCAC GGATGTCCTG
    GAAATAGAAG TTAAAGACAA ATTTGCAAAG AGCCGACCAA TCATCAAGCG TTTCCTGGGA
    AAACTCTCTA TGCCAGTTCA GCGTCTCTTG GAAAGACATG CCATAGGGGA CAGGGTTGTA
    AGCTACACTC TTGGTCGCAG GCTTCCTACA GATCATGTCA GTGGCCAGCT GCAGTTCCGA
    TTTGAGATAA CTTCTTCCAT TCATCAAGAT GATGAAGAGG TTACCATTAG TGCAGATCCT
    GAGCCAGCTG ACCTCCAGGA AAGCCCTGTG AACAACCCCA CAGAGGAAAG CCTCGGTGAG
    CCTCCATGCA TCAACACAGT GACCTCAGAA AATACAGTTC CTGAAGAGCT AAATGGATAC
    AGTGTGAACA ATATTGATAG CCCAGGGGCT GTCAAACCAC CCAGGGAAGT CAACCAGAAC
    AACATAAATG AAGAGCTAGC AGGAGATGAG GAGGTTGATG CCATGCCAGT TCCTGAACCC
    TTGGAATCCA TCCGAGGAGA AGTGCTGCCT TCTTTGAATG CGACAGACCT CACAGAGCAG
    CTGGGTCTGC TGGCTTGCTT GTCTCCTTCT GAGACTGAAG TTAGGGAAAA TAGCAAAGTC
    AATTCTGGTA CTCAGGGAGA TGATGGAGTA TTGAACAGTA CAGAAACTTT AGATGTTAGT
    GAGGTGAGTG CAGATGTATA TGCTGACAAA GACCTAGAGA AGAGTCATGA AGAAGAAGGG
    GCTTCAGCCC GAGGGGAGGA AGACAGCAAG CTACAGCTGA GGACCACAGC AAAGAGGAAA
    ACAAGGCCAT GCTCCCTGCC TGTGTCTGAA ATTGAGACCG TTATAGCTTC AGCTTGTGGT
    GAGCCAGAGA CCCCTCGCAC TCACTATATA CGAATCCACA ACCTGCTCCA CAGCCTGCCC
    TCAGCACAGA TGAGCAGTGA TGGGGAAGAA GAGCAAGATG CTGGCAATGG TGGGGAGAAT
    GATGAAGAGT CTACAGTCAA GGAAGTGTTG CAGAAGGAGA CAGTCAGTGA AAGTGAGACA
    ATGGCAGCAG AGCCTCCTCC AGAAAATGAG GCCGAAGAGA ACTTCAGCCA GTGCACAGTG
    CCTCCTGGGA CCTTAGACGA GCAACTCTCT GACTTAAATG ATGGACTGGT GGATGGGGAA
    AACCCTAGGA CAGGAAGCGA AAGCGAGTCA AGCTCCAGAC CCAATGGTGA CAACGAATGT
    GAGGCGTGCG ACACCTCTTG CTACAGCCCC TCCTGCTATA GTACCTCCTG CTACAGTACT
    TCCTGTTACA GCACCTCTTG CTACAGCACC TCCTGCTATG ACAGTAACAA TCGCTTCTCC
    AGCCATACCC GCTTCTCCTC TGTTGACAGT GCAAAGATTT CTGAGAGCAC GGTCTTTTCC
    TCACAGGATG AGGAGGAGGA GAATAGTGCT TTTGAATCGG TGCCAGACTC AGTACAGAGC
    CCTGAGCTGG ACAGGGAGCA AGTGGATGGC TCCTCCCAGT GGCCAGATGA ACTAGTAGCT
    CATGGTGGGA ATCCACAACT AGCCGCAGAA AGTCTAGACA CCCCAATAGC AGGTCCAAGC
    AGCAGAAGAG AAGGTGATTG TCCTTTACTC CATAATTCTC AGCCAGTCAG CCAACTTCCT
    TCTCTGAGGC CTGACCATCA TCACTACCCA ACTATCGATG AGCCTCTTCC ACCAACTGCA
    ACATCCTTGA GAAACCTTAC CCAGTTGGAT AACCAGAGAG CCCTCCCTTT AAAATTATGT
    GATGTTCACA GAGAGACTGT ATATTGGGAA GCTCGGATAG ACAGCCACGG GAGGGTGTTC
    TACGTTGACC ATGTCAACCG AACTACCACA TGGCAGCGCC CCACAGCTGC AGCAACACCA
    GATGGGATCC ACAGGTCTGG CTCTATCCAG CAAATGGAGC AGCTGAACCG GCGATATCAA
    AATATCCAGC GAACTATTGC AACAGAGCGG ACTGAAGATG ATGGTGTAGT AAACAACAGA
    GTGGAGAGAA TTCCCACTGG AGGAGGAAGT GACTCTGAGG CAGAGCCTTC CCAACCAAGC
    TCCGAAATTA GGAGGGAAGG TTCCCTTTCT CCTGTGAATT CTCAAAAAAT CACCTTATTG
    CTGCAGTCTC CAGCTGTGAA GTTTATCACC AATCCTGAGT TCTTCACTGT GCTGCATGCA
    AACTATAGTG CCTATCGAGT CTTCACTAAC AGTACCTGCT TGAAGCATAT GATCCTGAAG
    ATCCGTAGAG ATGCTCGTAA TTTTGAGCGC TATCAGCACA ACAGAGACCT TGTAAATTTT
    ATCAACATGT TTGCTGACAC TCGACTAGAG CTTCCTCGTG GCTGGGAAAT AAAAACTGAC
    CAGCAGGGCA AGTCTTTCTT CGTTGACCAC AACAGTCGTG CTACCACATT CATAGATCCC
    AGGATCCCAC TGCAGAATGG TCGTCTCCCC AATCACTTGA CTCACAGGCA ACATCTTCAG
    CGGCTTCGTA GCTACAGTGC TGGAGAGGCT TCTGAAGTCT CTCGGAACAG AGGAGTTACT
    CTGTTGGCCA GACCAGGCAA TAGTCTTGTA ACAGCTATTC GAAACCAGCA TCATTATGAG
    ACATTACCTC TGGCTTACAA TGACAAGATT GTTGCATTTC TACGCCAACC TAACATTTTT
    GAGATGCTGC AAGAGCGTCA GCCCAGTTTG GCCAGAAATC ATGCACTCAG GGAGAAGATC
    CATTATATTC GGACAGAGGG TACACATGGG CTTGAAAAGT TGTCCTGTGA TGCAGATTTA
    GTAATTCTGC TGAGCCTTTT TGAAGAAGAT ATCATGTCCT ACATACCACT CCAGGCCACT
    TTCCATCCTG GTTACAGTTT CTCTCCTCGC TGCTCACCCT GCTCATCTCC CCAAAATTCT
    CCAGGACTGC AGCGAGCAAG TGCAAGAGCA CCCTCTCCAT ACAGGAGAGA TTTTGAAGCC
    AAACTACGCA ATTTCTATCG AAAACTTGAA GCCAAAGGAT ATGGACAAGG TCCAGGTAAA
    ATTAAGTTAA TTATACGACG TGACCACTTA CTGGAAGGCA CCTTCAACCA GGTGATGGCA
    TATTCACGCA AGGAGCTCCA GCGAAACAAA CTCTACATCA CATTTGTTGG CGAGGAAGGG
    CTGGACTACA GTGGCCCCTC TCGAGAATTC TTTTTCCTTC TGTCTCAGGA GCTCTTCAAT
    CCATATTACG GGCTGTTTGA GTATTCAGCC AATGACACTT ACACTGTACA GATTAGCCCC
    ATGTCTGCCT TTGTTGAAAA TCACCTGGAA TGGTTCAGGT TCAGTGGTCG TATTCTTGGC
    CTTGCTCTCA TTCATCAGTA CCTTCTTGAT GCTTTCTTCA CAAGGCCGTT CTACAAAGCA
    CTATTAAGGC TGCCATGTGA TCTAAGTGAC TTAGAGTACC TGGATGAAGA ATTCCATCAG
    AGCTTGCAGT GGATGAAAGA TAACAACATC ACAGATATCC TGGATCTCAC CTTCACAGTG
    AATGAGGAGG TGTTTGGACA GGTGACAGAG AGAGAGTTGA AATCTGGAGG GGCAAATACA
    GCAGTGACAG AAAAGAACAA AAAAGAGTAC ATTGAGAGAA TGGTTAAATG GCGAGTGGAA
    CGAGGAGTTG TTCAACAAAC AGAGGCCCTG GTCCGTGGCT TCTATGAAGT TGTAGACTCA
    AGGCTTGTCT CTGTTTTTGA TGCCAGAGAA CTGGAGCTGG TTATAGCTGG CACTGCAGAA
    ATAGATCTCA ATGACTGGCG TAACAACACG GAATATCGTG GAGGTTACCA CGATGGACAC
    ATTGTAATAC GGTGGTTCTG GGCAGCAGTG GAGAGGTTTA ACAATGAACA GAGACTCCGG
    TTGTTGCAGT TTGTTACTGG AACATCAAGT GTGCCATATG AGGGCTTTGC AGCTCTCCGA
    GGAAGCAATG GGCTTCGGCG TTTCTGTATA GAGAAATGGG GGAAAATAAC CTCCCTTCCG
    AGGGCACATA CATGTTTCAA CCGCTTGGAT CTTCCACCTT ACCCCTCATA TTCTATGCTG
    TATGAAAAGC TGTTGACAGC TGTTGAAGAA ACTAGTACCT TTGGACTTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa31691
    Clone ID Related Accession (Same CDS sequence) XM_015281616.2 , XM_015281616.1
    Accession Version XM_015281616.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCTGCTCC ACCTGTGTAG TGTTAAGAAT CTGTACCAAA ACAGGTTCCT GGGCCTCGCA 
    GCCATGGCGT CTCCATCTAG GAACTCACAA AATCGGCGCC GGTGCAAAGA GCCACTCAGA
    TACAGCTATA ACCCTGACCA GTTTCATAAC ATGGACATCA GGAACAATTC CCATGAGACA
    GTCACCATTC CTAGGTCTAC CAGTGACACG GACCTTGTCA CTTCAGACAG CCGATCTACC
    CTGATGGTCA GCAGCTCCTA CTATTCCATT GGGCACTCAC AGGACCTAGT GATATACTGG
    GATATAAAGG AGGAAGTGGA TGCAGGGGAC TGGATTGGCA TGTATCTCAT AGATGAAGTC
    TTGTCTGAAA ACTTTTTGGA CTACAAGAAC CGTGGAGTCA ATGGCTCTCA TCGTGGTCAG
    ATTGTCTGGA AGATTGATGC CAGTTCTTAC TTTGTGGAGC CTGAAACAAA GATATGCTTC
    AAATACTATC ACGGTGTGAG TGGGGCACTA CGAGCCACAA CTCCAAGCGT CACGGTGAAA
    AACAATGCAG CTCCTCAGAT TTTTAAAAGC ATCACCAATG AAGATTCAAT CCAGGGACAG
    GGAAATCGGA GGCTGATAAG TTTTTCACTG TCAGATTTCC AAGCTTTTGG TCTGAAGAAA
    GGAATGTTTT TCAATCCAGA TCCTTATCTG AAGATTTCCA TCCAGCCTGG GAAACACAGC
    ATCTTCCCAG CACTTCCTCA TCATGGACAG GAGAAAAGGT CTAAGATCAT GTGTAATACA
    GTTAACCCAA TCTGGCAAGG AGAGCAATTC AGCTTTGTCT CACTGCCCAC GGATGTCCTG
    GAAATAGAAG TTAAAGACAA ATTTGCAAAG AGCCGACCAA TCATCAAGCG TTTCCTGGGA
    AAACTCTCTA TGCCAGTTCA GCGTCTCTTG GAAAGACATG CCATAGGGGA CAGGGTTGTA
    AGCTACACTC TTGGTCGCAG GCTTCCTACA GATCATGTCA GTGGCCAGCT GCAGTTCCGA
    TTTGAGATAA CTTCTTCCAT TCATCAAGAT GATGAAGAGG TTACCATTAG TGCAGATCCT
    GAGCCAGCTG ACCTCCAGGA AAGCCCTGTG AACAACCCCA CAGAGGAAAG CCTCGGTGAG
    CCTCCATGCA TCAACACAGT GACCTCAGAA AATACAGTTC CTGAAGAGCT AAATGGATAC
    AGTGTGAACA ATATTGATAG CCCAGGGGCT GTCAAACCAC CCAGGGAAGT CAACCAGAAC
    AACATAAATG AAGAGCTAGC AGGAGATGAG GAGGTTGATG CCATGCCAGT TCCTGAACCC
    TTGGAATCCA TCCGAGGAGA AGTGCTGCCT TCTTTGAATG CGACAGACCT CACAGAGCAG
    CTGGGTCTGC TGGCTTGCTT GTCTCCTTCT GAGACTGAAG TTAGGGAAAA TAGCAAAGTC
    AATTCTGGTA CTCAGGGAGA TGATGGAGTA TTGAACAGTA CAGAAACTTT AGATGTTAGT
    GAGGTGAGTG CAGATGTATA TGCTGACAAA GACCTAGAGA AGAGTCATGA AGAAGAAGGG
    GCTTCAGCCC GAGGGGAGGA AGACAGCAAG CTACAGCTGA GGACCACAGC AAAGAGGAAA
    ACAAGGCCAT GCTCCCTGCC TGTGTCTGAA ATTGAGACCG TTATAGCTTC AGCTTGTGGT
    GAGCCAGAGA CCCCTCGCAC TCACTATATA CGAATCCACA ACCTGCTCCA CAGCCTGCCC
    TCAGCACAGA TGAGCAGTGA TGGGGAAGAA GAGCAAGATG CTGGCAATGG TGGGGAGAAT
    GATGAAGAGT CTACAGTCAA GGAAGTGTTG CAGAAGGAGA CAGTCAGTGA AAGTGAGACA
    ATGGCAGCAG AGCCTCCTCC AGAAAATGAG GCCGAAGAGA ACTTCAGCCA GTGCACAGTG
    CCTCCTGGGA CCTTAGACGA GCAACTCTCT GACTTAAATG ATGGACTGGT GGATGGGGAA
    AACCCTAGGA CAGGAAGCGA AAGCGAGTCA AGCTCCAGAC CCAATGGTGA CAACGAATGT
    GAGGCGTGCG ACACCTCTTG CTACAGCCCC TCCTGCTATA GTACCTCCTG CTACAGTACT
    TCCTGTTACA GCACCTCTTG CTACAGCACC TCCTGCTATG ACAGTAACAA TCGCTTCTCC
    AGCCATACCC GCTTCTCCTC TGTTGACAGT GCAAAGATTT CTGAGAGCAC GGTCTTTTCC
    TCACAGGATG AGGAGGAGGA GAATAGTGCT TTTGAATCGG TGCCAGACTC AGTACAGAGC
    CCTGAGCTGG ACAGGGAGCA AGTGGATGGC TCCTCCCAGT GGCCAGATGA ACTAGTAGCT
    CATGGTGGGA ATCCACAACT AGCCGCAGAA AGTCTAGACA CCCCAATAGC AGGTCCAAGC
    AGCAGAAGAG AAGGTGATTG TCCTTTACTC CATAATTCTC AGCCAGTCAG CCAACTTCCT
    TCTCTGAGGC CTGACCATCA TCACTACCCA ACTATCGATG AGCCTCTTCC ACCAACTGCA
    ACATCCTTGA GAAACCTTAC CCAGTTGGAT AACCAGAGAG CCCTCCCTTT AAAATTATGT
    GATGTTCACA GAGAGACTGT ATATTGGGAA GCTCGGATAG ACAGCCACGG GAGGGTGTTC
    TACGTTGACC ATGTCAACCG AACTACCACA TGGCAGCGCC CCACAGCTGC AGCAACACCA
    GATGGGATCC ACAGGTCTGG CTCTATCCAG CAAATGGAGC AGCTGAACCG GCGATATCAA
    AATATCCAGC GAACTATTGC AACAGAGCGG ACTGAAGATG ATGGTGTAGT AAACAACAGA
    GTGGAGAGAA TTCCCACTGG AGGAGGAAGT GACTCTGAGG CAGAGCCTTC CCAACCAAGC
    TCCGAAATTA GGAGGGAAGG TTCCCTTTCT CCTGTGAATT CTCAAAAAAT CACCTTATTG
    CTGCAGTCTC CAGCTGTGAA GTTTATCACC AATCCTGAGT TCTTCACTGT GCTGCATGCA
    AACTATAGTG CCTATCGAGT CTTCACTAAC AGTACCTGCT TGAAGCATAT GATCCTGAAG
    ATCCGTAGAG ATGCTCGTAA TTTTGAGCGC TATCAGCACA ACAGAGACCT TGTAAATTTT
    ATCAACATGT TTGCTGACAC TCGACTAGAG CTTCCTCGTG GCTGGGAAAT AAAAACTGAC
    CAGCAGGGCA AGTCTTTCTT CGTTGACCAC AACAGTCGTG CTACCACATT CATAGATCCC
    AGGATCCCAC TGCAGAATGG TCGTCTCCCC AATCACTTGA CTCACAGGCA ACATCTTCAG
    CGGCTTCGTA GCTACAGTGC TGGAGAGGCT TCTGAAGTCT CTCGGAACAG AGGAGTTACT
    CTGTTGGCCA GACCAGGCAA TAGTCTTGTA ACAGCTATTC GAAACCAGCA TCATTATGAG
    ACATTACCTC TGGCTTACAA TGACAAGATT GTTGCATTTC TACGCCAACC TAACATTTTT
    GAGATGCTGC AAGAGCGTCA GCCCAGTTTG GCCAGAAATC ATGCACTCAG GGAGAAGATC
    CATTATATTC GGACAGAGGG TACACATGGG CTTGAAAAGT TGTCCTGTGA TGCAGATTTA
    GTAATTCTGC TGAGCCTTTT TGAAGAAGAT ATCATGTCCT ACATACCACT CCAGGCCACT
    TTCCATCCTG GTTACAGTTT CTCTCCTCGC TGCTCACCCT GCTCATCTCC CCAAAATTCT
    CCAGGACTGC AGCGAGCAAG TGCAAGAGCA CCCTCTCCAT ACAGGAGAGA TTTTGAAGCC
    AAACTACGCA ATTTCTATCG AAAACTTGAA GCCAAAGGAT ATGGACAAGG TCCAGGTAAA
    ATTAAGTTAA TTATACGACG TGACCACTTA CTGGAAGGCA CCTTCAACCA GGTGATGGCA
    TATTCACGCA AGGAGCTCCA GCGAAACAAA CTCTACATCA CATTTGTTGG CGAGGAAGGG
    CTGGACTACA GTGGCCCCTC TCGAGAATTC TTTTTCCTTC TGTCTCAGGA GCTCTTCAAT
    CCATATTACG GGCTGTTTGA GTATTCAGCC AATGACACTT ACACTGTACA GATTAGCCCC
    ATGTCTGCCT TTGTTGAAAA TCACCTGGAA TGGTTCAGGT TCAGTGGTCG TATTCTTGGC
    CTTGCTCTCA TTCATCAGTA CCTTCTTGAT GCTTTCTTCA CAAGGCCGTT CTACAAAGCA
    CTATTAAGGC TGCCATGTGA TCTAAGTGAC TTAGAGTACC TGGATGAAGA ATTCCATCAG
    AGCTTGCAGT GGATGAAAGA TAACAACATC ACAGATATCC TGGATCTCAC CTTCACAGTG
    AATGAGGAGG TGTTTGGACA GGTGACAGAG AGAGAGTTGA AATCTGGAGG GGCAAATACA
    GCAGTGACAG AAAAGAACAA AAAAGAGTAC ATTGAGAGAA TGGTTAAATG GCGAGTGGAA
    CGAGGAGTTG TTCAACAAAC AGAGGCCCTG GTCCGTGGCT TCTATGAAGT TGTAGACTCA
    AGGCTTGTCT CTGTTTTTGA TGCCAGAGAA CTGGAGCTGG TTATAGCTGG CACTGCAGAA
    ATAGATCTCA ATGACTGGCG TAACAACACG GAATATCGTG GAGGTTACCA CGATGGACAC
    ATTGTAATAC GGTGGTTCTG GGCAGCAGTG GAGAGGTTTA ACAATGAACA GAGACTCCGG
    TTGTTGCAGT TTGTTACTGG AACATCAAGT GTGCCATATG AGGGCTTTGC AGCTCTCCGA
    GGAAGCAATG GGCTTCGGCG TTTCTGTATA GAGAAATGGG GGAAAATAAC CTCCCTTCCG
    AGGGCACATA CATGTTTCAA CCGCTTGGAT CTTCCACCTT ACCCCTCATA TTCTATGCTG
    TATGAAAAGC TGTTGACAGC TGTTGAAGAA ACTAGTACCT TTGGACTTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137102.1
    CDS232..5145
    Translation

    Target ORF information:

    RefSeq Version XM_015281616.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281616.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCTGCTCC ACCTGTGTAG TGTTAAGAAT CTGTACCAAA ACAGGTTCCT GGGCCTCGCA 
    GCCATGGCGT CTCCATCTAG GAACTCACAA AATCGGCGCC GGTGCAAAGA GCCACTCAGA
    TACAGCTATA ACCCTGACCA GTTTCATAAC ATGGACATCA GGAACAATTC CCATGAGACA
    GTCACCATTC CTAGGTCTAC CAGTGACACG GACCTTGTCA CTTCAGACAG CCGATCTACC
    CTGATGGTCA GCAGCTCCTA CTATTCCATT GGGCACTCAC AGGACCTAGT GATATACTGG
    GATATAAAGG AGGAAGTGGA TGCAGGGGAC TGGATTGGCA TGTATCTCAT AGATGAAGTC
    TTGTCTGAAA ACTTTTTGGA CTACAAGAAC CGTGGAGTCA ATGGCTCTCA TCGTGGTCAG
    ATTGTCTGGA AGATTGATGC CAGTTCTTAC TTTGTGGAGC CTGAAACAAA GATATGCTTC
    AAATACTATC ACGGTGTGAG TGGGGCACTA CGAGCCACAA CTCCAAGCGT CACGGTGAAA
    AACAATGCAG CTCCTCAGAT TTTTAAAAGC ATCACCAATG AAGATTCAAT CCAGGGACAG
    GGAAATCGGA GGCTGATAAG TTTTTCACTG TCAGATTTCC AAGCTTTTGG TCTGAAGAAA
    GGAATGTTTT TCAATCCAGA TCCTTATCTG AAGATTTCCA TCCAGCCTGG GAAACACAGC
    ATCTTCCCAG CACTTCCTCA TCATGGACAG GAGAAAAGGT CTAAGATCAT GTGTAATACA
    GTTAACCCAA TCTGGCAAGG AGAGCAATTC AGCTTTGTCT CACTGCCCAC GGATGTCCTG
    GAAATAGAAG TTAAAGACAA ATTTGCAAAG AGCCGACCAA TCATCAAGCG TTTCCTGGGA
    AAACTCTCTA TGCCAGTTCA GCGTCTCTTG GAAAGACATG CCATAGGGGA CAGGGTTGTA
    AGCTACACTC TTGGTCGCAG GCTTCCTACA GATCATGTCA GTGGCCAGCT GCAGTTCCGA
    TTTGAGATAA CTTCTTCCAT TCATCAAGAT GATGAAGAGG TTACCATTAG TGCAGATCCT
    GAGCCAGCTG ACCTCCAGGA AAGCCCTGTG AACAACCCCA CAGAGGAAAG CCTCGGTGAG
    CCTCCATGCA TCAACACAGT GACCTCAGAA AATACAGTTC CTGAAGAGCT AAATGGATAC
    AGTGTGAACA ATATTGATAG CCCAGGGGCT GTCAAACCAC CCAGGGAAGT CAACCAGAAC
    AACATAAATG AAGAGCTAGC AGGAGATGAG GAGGTTGATG CCATGCCAGT TCCTGAACCC
    TTGGAATCCA TCCGAGGAGA AGTGCTGCCT TCTTTGAATG CGACAGACCT CACAGAGCAG
    CTGGGTCTGC TGGCTTGCTT GTCTCCTTCT GAGACTGAAG TTAGGGAAAA TAGCAAAGTC
    AATTCTGGTA CTCAGGGAGA TGATGGAGTA TTGAACAGTA CAGAAACTTT AGATGTTAGT
    GAGGTGAGTG CAGATGTATA TGCTGACAAA GACCTAGAGA AGAGTCATGA AGAAGAAGGG
    GCTTCAGCCC GAGGGGAGGA AGACAGCAAG CTACAGCTGA GGACCACAGC AAAGAGGAAA
    ACAAGGCCAT GCTCCCTGCC TGTGTCTGAA ATTGAGACCG TTATAGCTTC AGCTTGTGGT
    GAGCCAGAGA CCCCTCGCAC TCACTATATA CGAATCCACA ACCTGCTCCA CAGCCTGCCC
    TCAGCACAGA TGAGCAGTGA TGGGGAAGAA GAGCAAGATG CTGGCAATGG TGGGGAGAAT
    GATGAAGAGT CTACAGTCAA GGAAGTGTTG CAGAAGGAGA CAGTCAGTGA AAGTGAGACA
    ATGGCAGCAG AGCCTCCTCC AGAAAATGAG GCCGAAGAGA ACTTCAGCCA GTGCACAGTG
    CCTCCTGGGA CCTTAGACGA GCAACTCTCT GACTTAAATG ATGGACTGGT GGATGGGGAA
    AACCCTAGGA CAGGAAGCGA AAGCGAGTCA AGCTCCAGAC CCAATGGTGA CAACGAATGT
    GAGGCGTGCG ACACCTCTTG CTACAGCCCC TCCTGCTATA GTACCTCCTG CTACAGTACT
    TCCTGTTACA GCACCTCTTG CTACAGCACC TCCTGCTATG ACAGTAACAA TCGCTTCTCC
    AGCCATACCC GCTTCTCCTC TGTTGACAGT GCAAAGATTT CTGAGAGCAC GGTCTTTTCC
    TCACAGGATG AGGAGGAGGA GAATAGTGCT TTTGAATCGG TGCCAGACTC AGTACAGAGC
    CCTGAGCTGG ACAGGGAGCA AGTGGATGGC TCCTCCCAGT GGCCAGATGA ACTAGTAGCT
    CATGGTGGGA ATCCACAACT AGCCGCAGAA AGTCTAGACA CCCCAATAGC AGGTCCAAGC
    AGCAGAAGAG AAGGTGATTG TCCTTTACTC CATAATTCTC AGCCAGTCAG CCAACTTCCT
    TCTCTGAGGC CTGACCATCA TCACTACCCA ACTATCGATG AGCCTCTTCC ACCAACTGCA
    ACATCCTTGA GAAACCTTAC CCAGTTGGAT AACCAGAGAG CCCTCCCTTT AAAATTATGT
    GATGTTCACA GAGAGACTGT ATATTGGGAA GCTCGGATAG ACAGCCACGG GAGGGTGTTC
    TACGTTGACC ATGTCAACCG AACTACCACA TGGCAGCGCC CCACAGCTGC AGCAACACCA
    GATGGGATCC ACAGGTCTGG CTCTATCCAG CAAATGGAGC AGCTGAACCG GCGATATCAA
    AATATCCAGC GAACTATTGC AACAGAGCGG ACTGAAGATG ATGGTGTAGT AAACAACAGA
    GTGGAGAGAA TTCCCACTGG AGGAGGAAGT GACTCTGAGG CAGAGCCTTC CCAACCAAGC
    TCCGAAATTA GGAGGGAAGG TTCCCTTTCT CCTGTGAATT CTCAAAAAAT CACCTTATTG
    CTGCAGTCTC CAGCTGTGAA GTTTATCACC AATCCTGAGT TCTTCACTGT GCTGCATGCA
    AACTATAGTG CCTATCGAGT CTTCACTAAC AGTACCTGCT TGAAGCATAT GATCCTGAAG
    ATCCGTAGAG ATGCTCGTAA TTTTGAGCGC TATCAGCACA ACAGAGACCT TGTAAATTTT
    ATCAACATGT TTGCTGACAC TCGACTAGAG CTTCCTCGTG GCTGGGAAAT AAAAACTGAC
    CAGCAGGGCA AGTCTTTCTT CGTTGACCAC AACAGTCGTG CTACCACATT CATAGATCCC
    AGGATCCCAC TGCAGAATGG TCGTCTCCCC AATCACTTGA CTCACAGGCA ACATCTTCAG
    CGGCTTCGTA GCTACAGTGC TGGAGAGGCT TCTGAAGTCT CTCGGAACAG AGGAGTTACT
    CTGTTGGCCA GACCAGGCAA TAGTCTTGTA ACAGCTATTC GAAACCAGCA TCATTATGAG
    ACATTACCTC TGGCTTACAA TGACAAGATT GTTGCATTTC TACGCCAACC TAACATTTTT
    GAGATGCTGC AAGAGCGTCA GCCCAGTTTG GCCAGAAATC ATGCACTCAG GGAGAAGATC
    CATTATATTC GGACAGAGGG TACACATGGG CTTGAAAAGT TGTCCTGTGA TGCAGATTTA
    GTAATTCTGC TGAGCCTTTT TGAAGAAGAT ATCATGTCCT ACATACCACT CCAGGCCACT
    TTCCATCCTG GTTACAGTTT CTCTCCTCGC TGCTCACCCT GCTCATCTCC CCAAAATTCT
    CCAGGACTGC AGCGAGCAAG TGCAAGAGCA CCCTCTCCAT ACAGGAGAGA TTTTGAAGCC
    AAACTACGCA ATTTCTATCG AAAACTTGAA GCCAAAGGAT ATGGACAAGG TCCAGGTAAA
    ATTAAGTTAA TTATACGACG TGACCACTTA CTGGAAGGCA CCTTCAACCA GGTGATGGCA
    TATTCACGCA AGGAGCTCCA GCGAAACAAA CTCTACATCA CATTTGTTGG CGAGGAAGGG
    CTGGACTACA GTGGCCCCTC TCGAGAATTC TTTTTCCTTC TGTCTCAGGA GCTCTTCAAT
    CCATATTACG GGCTGTTTGA GTATTCAGCC AATGACACTT ACACTGTACA GATTAGCCCC
    ATGTCTGCCT TTGTTGAAAA TCACCTGGAA TGGTTCAGGT TCAGTGGTCG TATTCTTGGC
    CTTGCTCTCA TTCATCAGTA CCTTCTTGAT GCTTTCTTCA CAAGGCCGTT CTACAAAGCA
    CTATTAAGGC TGCCATGTGA TCTAAGTGAC TTAGAGTACC TGGATGAAGA ATTCCATCAG
    AGCTTGCAGT GGATGAAAGA TAACAACATC ACAGATATCC TGGATCTCAC CTTCACAGTG
    AATGAGGAGG TGTTTGGACA GGTGACAGAG AGAGAGTTGA AATCTGGAGG GGCAAATACA
    GCAGTGACAG AAAAGAACAA AAAAGAGTAC ATTGAGAGAA TGGTTAAATG GCGAGTGGAA
    CGAGGAGTTG TTCAACAAAC AGAGGCCCTG GTCCGTGGCT TCTATGAAGT TGTAGACTCA
    AGGCTTGTCT CTGTTTTTGA TGCCAGAGAA CTGGAGCTGG TTATAGCTGG CACTGCAGAA
    ATAGATCTCA ATGACTGGCG TAACAACACG GAATATCGTG GAGGTTACCA CGATGGACAC
    ATTGTAATAC GGTGGTTCTG GGCAGCAGTG GAGAGGTTTA ACAATGAACA GAGACTCCGG
    TTGTTGCAGT TTGTTACTGG AACATCAAGT GTGCCATATG AGGGCTTTGC AGCTCTCCGA
    GGAAGCAATG GGCTTCGGCG TTTCTGTATA GAGAAATGGG GGAAAATAAC CTCCCTTCCG
    AGGGCACATA CATGTTTCAA CCGCTTGGAT CTTCCACCTT ACCCCTCATA TTCTATGCTG
    TATGAAAAGC TGTTGACAGC TGTTGAAGAA ACTAGTACCT TTGGACTTGA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa31692
    Clone ID Related Accession (Same CDS sequence) XM_015281617.1 , XM_015281617.2
    Accession Version XM_015281617.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4851bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGATG TACTGGAAGA AATACAGCTA CCGGTTATGA AAGTCCCTCA GAATCTGTAC 
    CAAAACAGGT TCCTGGGCCT CGCAGCCATG GCGTCTCCAT CTAGGAACTC ACAAAATCGG
    CGCCGGTGCA AAGAGCCACT CAGATACAGC TATAACCCTG ACCAGTTTCA TAACATGGAC
    ATCAGGAACA ATTCCCATGA GACAGTCACC ATTCCTAGGT CTACCAGTGA CACGGACCTT
    GTCACTTCAG ACAGCCGATC TACCCTGATG GTCAGCAGCT CCTACTATTC CATTGGGCAC
    TCACAGGACC TAGTGATATA CTGGGATATA AAGGAGGAAG TGGATGCAGG GGACTGGATT
    GGCATGTATC TCATAGATGA AGTCTTGTCT GAAAACTTTT TGGACTACAA GAACCGTGGA
    GTCAATGGCT CTCATCGTGG TCAGATTGTC TGGAAGATTG ATGCCAGTTC TTACTTTGTG
    GAGCCTGAAA CAAAGATATG CTTCAAATAC TATCACGGTG TGAGTGGGGC ACTACGAGCC
    ACAACTCCAA GCGTCACGGT GAAAAACAAT GCAGCTCCTC AGATTTTTAA AAGCATCACC
    AATGAAGATT CAATCCAGGG ACAGGGAAAT CGGAGGCTGA TAAGTTTTTC ACTGTCAGAT
    TTCCAAGCTT TTGGTCTGAA GAAAGGAATG TTTTTCAATC CAGATCCTTA TCTGAAGATT
    TCCATCCAGC CTGGGAAACA CAGCATCTTC CCAGCACTTC CTCATCATGG ACAGGAGAAA
    AGGTCTAAGA TCATGTGTAA TACAGTTAAC CCAATCTGGC AAGGAGAGCA ATTCAGCTTT
    GTCTCACTGC CCACGGATGT CCTGGAAATA GAAGTTAAAG ACAAATTTGC AAAGAGCCGA
    CCAATCATCA AGCGTTTCCT GGGAAAACTC TCTATGCCAG TTCAGCGTCT CTTGGAAAGA
    CATGCCATAG GGGACAGGGT TGTAAGCTAC ACTCTTGGTC GCAGGCTTCC TACAGATCAT
    GTCAGTGGCC AGCTGCAGTT CCGATTTGAG ATAACTTCTT CCATTCATCA AGATGATGAA
    GAGGTTACCA TTAGTGCAGA TCCTGAGCCA GCTGACCTCC AGGAAAGCCC TGTGAACAAC
    CCCACAGAGG AAAGCCTCGG TGAGCCTCCA TGCATCAACA CAGTGACCTC AGAAAATACA
    GTTCCTGAAG AGCTAAATGG ATACAGTGTG AACAATATTG ATAGCCCAGG GGCTGTCAAA
    CCACCCAGGG AAGTCAACCA GAACAACATA AATGAAGAGC TAGCAGGAGA TGAGGAGGTT
    GATGCCATGC CAGTTCCTGA ACCCTTGGAA TCCATCCGAG GAGAAGTGCT GCCTTCTTTG
    AATGCGACAG ACCTCACAGA GCAGCTGGGT CTGCTGGCTT GCTTGTCTCC TTCTGAGACT
    GAAGTTAGGG AAAATAGCAA AGTCAATTCT GGTACTCAGG GAGATGATGG AGTATTGAAC
    AGTACAGAAA CTTTAGATGT TAGTGAGGTG AGTGCAGATG TATATGCTGA CAAAGACCTA
    GAGAAGAGTC ATGAAGAAGA AGGGGCTTCA GCCCGAGGGG AGGAAGACAG CAAGCTACAG
    CTGAGGACCA CAGCAAAGAG GAAAACAAGG CCATGCTCCC TGCCTGTGTC TGAAATTGAG
    ACCGTTATAG CTTCAGCTTG TGGTGAGCCA GAGACCCCTC GCACTCACTA TATACGAATC
    CACAACCTGC TCCACAGCCT GCCCTCAGCA CAGATGAGCA GTGATGGGGA AGAAGAGCAA
    GATGCTGGCA ATGGTGGGGA GAATGATGAA GAGTCTACAG TCAAGGAAGT GTTGCAGAAG
    GAGACAGTCA GTGAAAGTGA GACAATGGCA GCAGAGCCTC CTCCAGAAAA TGAGGCCGAA
    GAGAACTTCA GCCAGTGCAC AGTGCCTCCT GGGACCTTAG ACGAGCAACT CTCTGACTTA
    AATGATGGAC TGGTGGATGG GGAAAACCCT AGGACAGGAA GCGAAAGCGA GTCAAGCTCC
    AGACCCAATG GTGACAACGA ATGTGAGGCG TGCGACACCT CTTGCTACAG CCCCTCCTGC
    TATAGTACCT CCTGCTACAG TACTTCCTGT TACAGCACCT CTTGCTACAG CACCTCCTGC
    TATGACAGTA ACAATCGCTT CTCCAGCCAT ACCCGCTTCT CCTCTGTTGA CAGTGCAAAG
    ATTTCTGAGA GCACGGTCTT TTCCTCACAG GATGAGGAGG AGGAGAATAG TGCTTTTGAA
    TCGGTGCCAG ACTCAGTACA GAGCCCTGAG CTGGACAGGG AGCAAGTGGA TGGCTCCTCC
    CAGTGGCCAG ATGAACTAGT AGCTCATGGT GGGAATCCAC AACTAGCCGC AGAAAGTCTA
    GACACCCCAA TAGCAGGTCC AAGCAGCAGA AGAGAAGGTG ATTGTCCTTT ACTCCATAAT
    TCTCAGCCAG TCAGCCAACT TCCTTCTCTG AGGCCTGACC ATCATCACTA CCCAACTATC
    GATGAGCCTC TTCCACCAAA TTGGGAAGCT CGGATAGACA GCCACGGGAG GGTGTTCTAC
    GTTGACCATG TCAACCGAAC TACCACATGG CAGCGCCCCA CAGCTGCAGC AACACCAGAT
    GGGATCCACA GGTCTGGCTC TATCCAGCAA ATGGAGCAGC TGAACCGGCG ATATCAAAAT
    ATCCAGCGAA CTATTGCAAC AGAGCGGACT GAAGATGATG GTGTAGTAAA CAACAGAGTG
    GAGAGAATTC CCACTGGAGG AGGAAGTGAC TCTGAGGCAG AGCCTTCCCA ACCAAGCTCC
    GAAATTAGGA GGGAAGGTTC CCTTTCTCCT GTGAATTCTC AAAAAATCAC CTTATTGCTG
    CAGTCTCCAG CTGTGAAGTT TATCACCAAT CCTGAGTTCT TCACTGTGCT GCATGCAAAC
    TATAGTGCCT ATCGAGTCTT CACTAACAGT ACCTGCTTGA AGCATATGAT CCTGAAGATC
    CGTAGAGATG CTCGTAATTT TGAGCGCTAT CAGCACAACA GAGACCTTGT AAATTTTATC
    AACATGTTTG CTGACACTCG ACTAGAGCTT CCTCGTGGCT GGGAAATAAA AACTGACCAG
    CAGGGCAAGT CTTTCTTCGT TGACCACAAC AGTCGTGCTA CCACATTCAT AGATCCCAGG
    ATCCCACTGC AGAATGGTCG TCTCCCCAAT CACTTGACTC ACAGGCAACA TCTTCAGCGG
    CTTCGTAGCT ACAGTGCTGG AGAGGCTTCT GAAGTCTCTC GGAACAGAGG AGTTACTCTG
    TTGGCCAGAC CAGGCAATAG TCTTGTAACA GCTATTCGAA ACCAGCATCA TTATGAGACA
    TTACCTCTGG CTTACAATGA CAAGATTGTT GCATTTCTAC GCCAACCTAA CATTTTTGAG
    ATGCTGCAAG AGCGTCAGCC CAGTTTGGCC AGAAATCATG CACTCAGGGA GAAGATCCAT
    TATATTCGGA CAGAGGGTAC ACATGGGCTT GAAAAGTTGT CCTGTGATGC AGATTTAGTA
    ATTCTGCTGA GCCTTTTTGA AGAAGATATC ATGTCCTACA TACCACTCCA GGCCACTTTC
    CATCCTGGTT ACAGTTTCTC TCCTCGCTGC TCACCCTGCT CATCTCCCCA AAATTCTCCA
    GGACTGCAGC GAGCAAGTGC AAGAGCACCC TCTCCATACA GGAGAGATTT TGAAGCCAAA
    CTACGCAATT TCTATCGAAA ACTTGAAGCC AAAGGATATG GACAAGGTCC AGGTAAAATT
    AAGTTAATTA TACGACGTGA CCACTTACTG GAAGGCACCT TCAACCAGGT GATGGCATAT
    TCACGCAAGG AGCTCCAGCG AAACAAACTC TACATCACAT TTGTTGGCGA GGAAGGGCTG
    GACTACAGTG GCCCCTCTCG AGAATTCTTT TTCCTTCTGT CTCAGGAGCT CTTCAATCCA
    TATTACGGGC TGTTTGAGTA TTCAGCCAAT GACACTTACA CTGTACAGAT TAGCCCCATG
    TCTGCCTTTG TTGAAAATCA CCTGGAATGG TTCAGGTTCA GTGGTCGTAT TCTTGGCCTT
    GCTCTCATTC ATCAGTACCT TCTTGATGCT TTCTTCACAA GGCCGTTCTA CAAAGCACTA
    TTAAGGCTGC CATGTGATCT AAGTGACTTA GAGTACCTGG ATGAAGAATT CCATCAGAGC
    TTGCAGTGGA TGAAAGATAA CAACATCACA GATATCCTGG ATCTCACCTT CACAGTGAAT
    GAGGAGGTGT TTGGACAGGT GACAGAGAGA GAGTTGAAAT CTGGAGGGGC AAATACAGCA
    GTGACAGAAA AGAACAAAAA AGAGTACATT GAGAGAATGG TTAAATGGCG AGTGGAACGA
    GGAGTTGTTC AACAAACAGA GGCCCTGGTC CGTGGCTTCT ATGAAGTTGT AGACTCAAGG
    CTTGTCTCTG TTTTTGATGC CAGAGAACTG GAGCTGGTTA TAGCTGGCAC TGCAGAAATA
    GATCTCAATG ACTGGCGTAA CAACACGGAA TATCGTGGAG GTTACCACGA TGGACACATT
    GTAATACGGT GGTTCTGGGC AGCAGTGGAG AGGTTTAACA ATGAACAGAG ACTCCGGTTG
    TTGCAGTTTG TTACTGGAAC ATCAAGTGTG CCATATGAGG GCTTTGCAGC TCTCCGAGGA
    AGCAATGGGC TTCGGCGTTT CTGTATAGAG AAATGGGGGA AAATAACCTC CCTTCCGAGG
    GCACATACAT GTTTCAACCG CTTGGATCTT CCACCTTACC CCTCATATTC TATGCTGTAT
    GAAAAGCTGT TGACAGCTGT TGAAGAAACT AGTACCTTTG GACTTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137103.1
    CDS46..4896
    Translation

    Target ORF information:

    RefSeq Version XM_015281617.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281617.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGATG TACTGGAAGA AATACAGCTA CCGGTTATGA AAGTCCCTCA GAATCTGTAC 
    CAAAACAGGT TCCTGGGCCT CGCAGCCATG GCGTCTCCAT CTAGGAACTC ACAAAATCGG
    CGCCGGTGCA AAGAGCCACT CAGATACAGC TATAACCCTG ACCAGTTTCA TAACATGGAC
    ATCAGGAACA ATTCCCATGA GACAGTCACC ATTCCTAGGT CTACCAGTGA CACGGACCTT
    GTCACTTCAG ACAGCCGATC TACCCTGATG GTCAGCAGCT CCTACTATTC CATTGGGCAC
    TCACAGGACC TAGTGATATA CTGGGATATA AAGGAGGAAG TGGATGCAGG GGACTGGATT
    GGCATGTATC TCATAGATGA AGTCTTGTCT GAAAACTTTT TGGACTACAA GAACCGTGGA
    GTCAATGGCT CTCATCGTGG TCAGATTGTC TGGAAGATTG ATGCCAGTTC TTACTTTGTG
    GAGCCTGAAA CAAAGATATG CTTCAAATAC TATCACGGTG TGAGTGGGGC ACTACGAGCC
    ACAACTCCAA GCGTCACGGT GAAAAACAAT GCAGCTCCTC AGATTTTTAA AAGCATCACC
    AATGAAGATT CAATCCAGGG ACAGGGAAAT CGGAGGCTGA TAAGTTTTTC ACTGTCAGAT
    TTCCAAGCTT TTGGTCTGAA GAAAGGAATG TTTTTCAATC CAGATCCTTA TCTGAAGATT
    TCCATCCAGC CTGGGAAACA CAGCATCTTC CCAGCACTTC CTCATCATGG ACAGGAGAAA
    AGGTCTAAGA TCATGTGTAA TACAGTTAAC CCAATCTGGC AAGGAGAGCA ATTCAGCTTT
    GTCTCACTGC CCACGGATGT CCTGGAAATA GAAGTTAAAG ACAAATTTGC AAAGAGCCGA
    CCAATCATCA AGCGTTTCCT GGGAAAACTC TCTATGCCAG TTCAGCGTCT CTTGGAAAGA
    CATGCCATAG GGGACAGGGT TGTAAGCTAC ACTCTTGGTC GCAGGCTTCC TACAGATCAT
    GTCAGTGGCC AGCTGCAGTT CCGATTTGAG ATAACTTCTT CCATTCATCA AGATGATGAA
    GAGGTTACCA TTAGTGCAGA TCCTGAGCCA GCTGACCTCC AGGAAAGCCC TGTGAACAAC
    CCCACAGAGG AAAGCCTCGG TGAGCCTCCA TGCATCAACA CAGTGACCTC AGAAAATACA
    GTTCCTGAAG AGCTAAATGG ATACAGTGTG AACAATATTG ATAGCCCAGG GGCTGTCAAA
    CCACCCAGGG AAGTCAACCA GAACAACATA AATGAAGAGC TAGCAGGAGA TGAGGAGGTT
    GATGCCATGC CAGTTCCTGA ACCCTTGGAA TCCATCCGAG GAGAAGTGCT GCCTTCTTTG
    AATGCGACAG ACCTCACAGA GCAGCTGGGT CTGCTGGCTT GCTTGTCTCC TTCTGAGACT
    GAAGTTAGGG AAAATAGCAA AGTCAATTCT GGTACTCAGG GAGATGATGG AGTATTGAAC
    AGTACAGAAA CTTTAGATGT TAGTGAGGTG AGTGCAGATG TATATGCTGA CAAAGACCTA
    GAGAAGAGTC ATGAAGAAGA AGGGGCTTCA GCCCGAGGGG AGGAAGACAG CAAGCTACAG
    CTGAGGACCA CAGCAAAGAG GAAAACAAGG CCATGCTCCC TGCCTGTGTC TGAAATTGAG
    ACCGTTATAG CTTCAGCTTG TGGTGAGCCA GAGACCCCTC GCACTCACTA TATACGAATC
    CACAACCTGC TCCACAGCCT GCCCTCAGCA CAGATGAGCA GTGATGGGGA AGAAGAGCAA
    GATGCTGGCA ATGGTGGGGA GAATGATGAA GAGTCTACAG TCAAGGAAGT GTTGCAGAAG
    GAGACAGTCA GTGAAAGTGA GACAATGGCA GCAGAGCCTC CTCCAGAAAA TGAGGCCGAA
    GAGAACTTCA GCCAGTGCAC AGTGCCTCCT GGGACCTTAG ACGAGCAACT CTCTGACTTA
    AATGATGGAC TGGTGGATGG GGAAAACCCT AGGACAGGAA GCGAAAGCGA GTCAAGCTCC
    AGACCCAATG GTGACAACGA ATGTGAGGCG TGCGACACCT CTTGCTACAG CCCCTCCTGC
    TATAGTACCT CCTGCTACAG TACTTCCTGT TACAGCACCT CTTGCTACAG CACCTCCTGC
    TATGACAGTA ACAATCGCTT CTCCAGCCAT ACCCGCTTCT CCTCTGTTGA CAGTGCAAAG
    ATTTCTGAGA GCACGGTCTT TTCCTCACAG GATGAGGAGG AGGAGAATAG TGCTTTTGAA
    TCGGTGCCAG ACTCAGTACA GAGCCCTGAG CTGGACAGGG AGCAAGTGGA TGGCTCCTCC
    CAGTGGCCAG ATGAACTAGT AGCTCATGGT GGGAATCCAC AACTAGCCGC AGAAAGTCTA
    GACACCCCAA TAGCAGGTCC AAGCAGCAGA AGAGAAGGTG ATTGTCCTTT ACTCCATAAT
    TCTCAGCCAG TCAGCCAACT TCCTTCTCTG AGGCCTGACC ATCATCACTA CCCAACTATC
    GATGAGCCTC TTCCACCAAA TTGGGAAGCT CGGATAGACA GCCACGGGAG GGTGTTCTAC
    GTTGACCATG TCAACCGAAC TACCACATGG CAGCGCCCCA CAGCTGCAGC AACACCAGAT
    GGGATCCACA GGTCTGGCTC TATCCAGCAA ATGGAGCAGC TGAACCGGCG ATATCAAAAT
    ATCCAGCGAA CTATTGCAAC AGAGCGGACT GAAGATGATG GTGTAGTAAA CAACAGAGTG
    GAGAGAATTC CCACTGGAGG AGGAAGTGAC TCTGAGGCAG AGCCTTCCCA ACCAAGCTCC
    GAAATTAGGA GGGAAGGTTC CCTTTCTCCT GTGAATTCTC AAAAAATCAC CTTATTGCTG
    CAGTCTCCAG CTGTGAAGTT TATCACCAAT CCTGAGTTCT TCACTGTGCT GCATGCAAAC
    TATAGTGCCT ATCGAGTCTT CACTAACAGT ACCTGCTTGA AGCATATGAT CCTGAAGATC
    CGTAGAGATG CTCGTAATTT TGAGCGCTAT CAGCACAACA GAGACCTTGT AAATTTTATC
    AACATGTTTG CTGACACTCG ACTAGAGCTT CCTCGTGGCT GGGAAATAAA AACTGACCAG
    CAGGGCAAGT CTTTCTTCGT TGACCACAAC AGTCGTGCTA CCACATTCAT AGATCCCAGG
    ATCCCACTGC AGAATGGTCG TCTCCCCAAT CACTTGACTC ACAGGCAACA TCTTCAGCGG
    CTTCGTAGCT ACAGTGCTGG AGAGGCTTCT GAAGTCTCTC GGAACAGAGG AGTTACTCTG
    TTGGCCAGAC CAGGCAATAG TCTTGTAACA GCTATTCGAA ACCAGCATCA TTATGAGACA
    TTACCTCTGG CTTACAATGA CAAGATTGTT GCATTTCTAC GCCAACCTAA CATTTTTGAG
    ATGCTGCAAG AGCGTCAGCC CAGTTTGGCC AGAAATCATG CACTCAGGGA GAAGATCCAT
    TATATTCGGA CAGAGGGTAC ACATGGGCTT GAAAAGTTGT CCTGTGATGC AGATTTAGTA
    ATTCTGCTGA GCCTTTTTGA AGAAGATATC ATGTCCTACA TACCACTCCA GGCCACTTTC
    CATCCTGGTT ACAGTTTCTC TCCTCGCTGC TCACCCTGCT CATCTCCCCA AAATTCTCCA
    GGACTGCAGC GAGCAAGTGC AAGAGCACCC TCTCCATACA GGAGAGATTT TGAAGCCAAA
    CTACGCAATT TCTATCGAAA ACTTGAAGCC AAAGGATATG GACAAGGTCC AGGTAAAATT
    AAGTTAATTA TACGACGTGA CCACTTACTG GAAGGCACCT TCAACCAGGT GATGGCATAT
    TCACGCAAGG AGCTCCAGCG AAACAAACTC TACATCACAT TTGTTGGCGA GGAAGGGCTG
    GACTACAGTG GCCCCTCTCG AGAATTCTTT TTCCTTCTGT CTCAGGAGCT CTTCAATCCA
    TATTACGGGC TGTTTGAGTA TTCAGCCAAT GACACTTACA CTGTACAGAT TAGCCCCATG
    TCTGCCTTTG TTGAAAATCA CCTGGAATGG TTCAGGTTCA GTGGTCGTAT TCTTGGCCTT
    GCTCTCATTC ATCAGTACCT TCTTGATGCT TTCTTCACAA GGCCGTTCTA CAAAGCACTA
    TTAAGGCTGC CATGTGATCT AAGTGACTTA GAGTACCTGG ATGAAGAATT CCATCAGAGC
    TTGCAGTGGA TGAAAGATAA CAACATCACA GATATCCTGG ATCTCACCTT CACAGTGAAT
    GAGGAGGTGT TTGGACAGGT GACAGAGAGA GAGTTGAAAT CTGGAGGGGC AAATACAGCA
    GTGACAGAAA AGAACAAAAA AGAGTACATT GAGAGAATGG TTAAATGGCG AGTGGAACGA
    GGAGTTGTTC AACAAACAGA GGCCCTGGTC CGTGGCTTCT ATGAAGTTGT AGACTCAAGG
    CTTGTCTCTG TTTTTGATGC CAGAGAACTG GAGCTGGTTA TAGCTGGCAC TGCAGAAATA
    GATCTCAATG ACTGGCGTAA CAACACGGAA TATCGTGGAG GTTACCACGA TGGACACATT
    GTAATACGGT GGTTCTGGGC AGCAGTGGAG AGGTTTAACA ATGAACAGAG ACTCCGGTTG
    TTGCAGTTTG TTACTGGAAC ATCAAGTGTG CCATATGAGG GCTTTGCAGC TCTCCGAGGA
    AGCAATGGGC TTCGGCGTTT CTGTATAGAG AAATGGGGGA AAATAACCTC CCTTCCGAGG
    GCACATACAT GTTTCAACCG CTTGGATCTT CCACCTTACC CCTCATATTC TATGCTGTAT
    GAAAAGCTGT TGACAGCTGT TGAAGAAACT AGTACCTTTG GACTTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa31692
    Clone ID Related Accession (Same CDS sequence) XM_015281617.1 , XM_015281617.2
    Accession Version XM_015281617.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4851bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281617.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGATG TACTGGAAGA AATACAGCTA CCGGTTATGA AAGTCCCTCA GAATCTGTAC 
    CAAAACAGGT TCCTGGGCCT CGCAGCCATG GCGTCTCCAT CTAGGAACTC ACAAAATCGG
    CGCCGGTGCA AAGAGCCACT CAGATACAGC TATAACCCTG ACCAGTTTCA TAACATGGAC
    ATCAGGAACA ATTCCCATGA GACAGTCACC ATTCCTAGGT CTACCAGTGA CACGGACCTT
    GTCACTTCAG ACAGCCGATC TACCCTGATG GTCAGCAGCT CCTACTATTC CATTGGGCAC
    TCACAGGACC TAGTGATATA CTGGGATATA AAGGAGGAAG TGGATGCAGG GGACTGGATT
    GGCATGTATC TCATAGATGA AGTCTTGTCT GAAAACTTTT TGGACTACAA GAACCGTGGA
    GTCAATGGCT CTCATCGTGG TCAGATTGTC TGGAAGATTG ATGCCAGTTC TTACTTTGTG
    GAGCCTGAAA CAAAGATATG CTTCAAATAC TATCACGGTG TGAGTGGGGC ACTACGAGCC
    ACAACTCCAA GCGTCACGGT GAAAAACAAT GCAGCTCCTC AGATTTTTAA AAGCATCACC
    AATGAAGATT CAATCCAGGG ACAGGGAAAT CGGAGGCTGA TAAGTTTTTC ACTGTCAGAT
    TTCCAAGCTT TTGGTCTGAA GAAAGGAATG TTTTTCAATC CAGATCCTTA TCTGAAGATT
    TCCATCCAGC CTGGGAAACA CAGCATCTTC CCAGCACTTC CTCATCATGG ACAGGAGAAA
    AGGTCTAAGA TCATGTGTAA TACAGTTAAC CCAATCTGGC AAGGAGAGCA ATTCAGCTTT
    GTCTCACTGC CCACGGATGT CCTGGAAATA GAAGTTAAAG ACAAATTTGC AAAGAGCCGA
    CCAATCATCA AGCGTTTCCT GGGAAAACTC TCTATGCCAG TTCAGCGTCT CTTGGAAAGA
    CATGCCATAG GGGACAGGGT TGTAAGCTAC ACTCTTGGTC GCAGGCTTCC TACAGATCAT
    GTCAGTGGCC AGCTGCAGTT CCGATTTGAG ATAACTTCTT CCATTCATCA AGATGATGAA
    GAGGTTACCA TTAGTGCAGA TCCTGAGCCA GCTGACCTCC AGGAAAGCCC TGTGAACAAC
    CCCACAGAGG AAAGCCTCGG TGAGCCTCCA TGCATCAACA CAGTGACCTC AGAAAATACA
    GTTCCTGAAG AGCTAAATGG ATACAGTGTG AACAATATTG ATAGCCCAGG GGCTGTCAAA
    CCACCCAGGG AAGTCAACCA GAACAACATA AATGAAGAGC TAGCAGGAGA TGAGGAGGTT
    GATGCCATGC CAGTTCCTGA ACCCTTGGAA TCCATCCGAG GAGAAGTGCT GCCTTCTTTG
    AATGCGACAG ACCTCACAGA GCAGCTGGGT CTGCTGGCTT GCTTGTCTCC TTCTGAGACT
    GAAGTTAGGG AAAATAGCAA AGTCAATTCT GGTACTCAGG GAGATGATGG AGTATTGAAC
    AGTACAGAAA CTTTAGATGT TAGTGAGGTG AGTGCAGATG TATATGCTGA CAAAGACCTA
    GAGAAGAGTC ATGAAGAAGA AGGGGCTTCA GCCCGAGGGG AGGAAGACAG CAAGCTACAG
    CTGAGGACCA CAGCAAAGAG GAAAACAAGG CCATGCTCCC TGCCTGTGTC TGAAATTGAG
    ACCGTTATAG CTTCAGCTTG TGGTGAGCCA GAGACCCCTC GCACTCACTA TATACGAATC
    CACAACCTGC TCCACAGCCT GCCCTCAGCA CAGATGAGCA GTGATGGGGA AGAAGAGCAA
    GATGCTGGCA ATGGTGGGGA GAATGATGAA GAGTCTACAG TCAAGGAAGT GTTGCAGAAG
    GAGACAGTCA GTGAAAGTGA GACAATGGCA GCAGAGCCTC CTCCAGAAAA TGAGGCCGAA
    GAGAACTTCA GCCAGTGCAC AGTGCCTCCT GGGACCTTAG ACGAGCAACT CTCTGACTTA
    AATGATGGAC TGGTGGATGG GGAAAACCCT AGGACAGGAA GCGAAAGCGA GTCAAGCTCC
    AGACCCAATG GTGACAACGA ATGTGAGGCG TGCGACACCT CTTGCTACAG CCCCTCCTGC
    TATAGTACCT CCTGCTACAG TACTTCCTGT TACAGCACCT CTTGCTACAG CACCTCCTGC
    TATGACAGTA ACAATCGCTT CTCCAGCCAT ACCCGCTTCT CCTCTGTTGA CAGTGCAAAG
    ATTTCTGAGA GCACGGTCTT TTCCTCACAG GATGAGGAGG AGGAGAATAG TGCTTTTGAA
    TCGGTGCCAG ACTCAGTACA GAGCCCTGAG CTGGACAGGG AGCAAGTGGA TGGCTCCTCC
    CAGTGGCCAG ATGAACTAGT AGCTCATGGT GGGAATCCAC AACTAGCCGC AGAAAGTCTA
    GACACCCCAA TAGCAGGTCC AAGCAGCAGA AGAGAAGGTG ATTGTCCTTT ACTCCATAAT
    TCTCAGCCAG TCAGCCAACT TCCTTCTCTG AGGCCTGACC ATCATCACTA CCCAACTATC
    GATGAGCCTC TTCCACCAAA TTGGGAAGCT CGGATAGACA GCCACGGGAG GGTGTTCTAC
    GTTGACCATG TCAACCGAAC TACCACATGG CAGCGCCCCA CAGCTGCAGC AACACCAGAT
    GGGATCCACA GGTCTGGCTC TATCCAGCAA ATGGAGCAGC TGAACCGGCG ATATCAAAAT
    ATCCAGCGAA CTATTGCAAC AGAGCGGACT GAAGATGATG GTGTAGTAAA CAACAGAGTG
    GAGAGAATTC CCACTGGAGG AGGAAGTGAC TCTGAGGCAG AGCCTTCCCA ACCAAGCTCC
    GAAATTAGGA GGGAAGGTTC CCTTTCTCCT GTGAATTCTC AAAAAATCAC CTTATTGCTG
    CAGTCTCCAG CTGTGAAGTT TATCACCAAT CCTGAGTTCT TCACTGTGCT GCATGCAAAC
    TATAGTGCCT ATCGAGTCTT CACTAACAGT ACCTGCTTGA AGCATATGAT CCTGAAGATC
    CGTAGAGATG CTCGTAATTT TGAGCGCTAT CAGCACAACA GAGACCTTGT AAATTTTATC
    AACATGTTTG CTGACACTCG ACTAGAGCTT CCTCGTGGCT GGGAAATAAA AACTGACCAG
    CAGGGCAAGT CTTTCTTCGT TGACCACAAC AGTCGTGCTA CCACATTCAT AGATCCCAGG
    ATCCCACTGC AGAATGGTCG TCTCCCCAAT CACTTGACTC ACAGGCAACA TCTTCAGCGG
    CTTCGTAGCT ACAGTGCTGG AGAGGCTTCT GAAGTCTCTC GGAACAGAGG AGTTACTCTG
    TTGGCCAGAC CAGGCAATAG TCTTGTAACA GCTATTCGAA ACCAGCATCA TTATGAGACA
    TTACCTCTGG CTTACAATGA CAAGATTGTT GCATTTCTAC GCCAACCTAA CATTTTTGAG
    ATGCTGCAAG AGCGTCAGCC CAGTTTGGCC AGAAATCATG CACTCAGGGA GAAGATCCAT
    TATATTCGGA CAGAGGGTAC ACATGGGCTT GAAAAGTTGT CCTGTGATGC AGATTTAGTA
    ATTCTGCTGA GCCTTTTTGA AGAAGATATC ATGTCCTACA TACCACTCCA GGCCACTTTC
    CATCCTGGTT ACAGTTTCTC TCCTCGCTGC TCACCCTGCT CATCTCCCCA AAATTCTCCA
    GGACTGCAGC GAGCAAGTGC AAGAGCACCC TCTCCATACA GGAGAGATTT TGAAGCCAAA
    CTACGCAATT TCTATCGAAA ACTTGAAGCC AAAGGATATG GACAAGGTCC AGGTAAAATT
    AAGTTAATTA TACGACGTGA CCACTTACTG GAAGGCACCT TCAACCAGGT GATGGCATAT
    TCACGCAAGG AGCTCCAGCG AAACAAACTC TACATCACAT TTGTTGGCGA GGAAGGGCTG
    GACTACAGTG GCCCCTCTCG AGAATTCTTT TTCCTTCTGT CTCAGGAGCT CTTCAATCCA
    TATTACGGGC TGTTTGAGTA TTCAGCCAAT GACACTTACA CTGTACAGAT TAGCCCCATG
    TCTGCCTTTG TTGAAAATCA CCTGGAATGG TTCAGGTTCA GTGGTCGTAT TCTTGGCCTT
    GCTCTCATTC ATCAGTACCT TCTTGATGCT TTCTTCACAA GGCCGTTCTA CAAAGCACTA
    TTAAGGCTGC CATGTGATCT AAGTGACTTA GAGTACCTGG ATGAAGAATT CCATCAGAGC
    TTGCAGTGGA TGAAAGATAA CAACATCACA GATATCCTGG ATCTCACCTT CACAGTGAAT
    GAGGAGGTGT TTGGACAGGT GACAGAGAGA GAGTTGAAAT CTGGAGGGGC AAATACAGCA
    GTGACAGAAA AGAACAAAAA AGAGTACATT GAGAGAATGG TTAAATGGCG AGTGGAACGA
    GGAGTTGTTC AACAAACAGA GGCCCTGGTC CGTGGCTTCT ATGAAGTTGT AGACTCAAGG
    CTTGTCTCTG TTTTTGATGC CAGAGAACTG GAGCTGGTTA TAGCTGGCAC TGCAGAAATA
    GATCTCAATG ACTGGCGTAA CAACACGGAA TATCGTGGAG GTTACCACGA TGGACACATT
    GTAATACGGT GGTTCTGGGC AGCAGTGGAG AGGTTTAACA ATGAACAGAG ACTCCGGTTG
    TTGCAGTTTG TTACTGGAAC ATCAAGTGTG CCATATGAGG GCTTTGCAGC TCTCCGAGGA
    AGCAATGGGC TTCGGCGTTT CTGTATAGAG AAATGGGGGA AAATAACCTC CCTTCCGAGG
    GCACATACAT GTTTCAACCG CTTGGATCTT CCACCTTACC CCTCATATTC TATGCTGTAT
    GAAAAGCTGT TGACAGCTGT TGAAGAAACT AGTACCTTTG GACTTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137103.1
    CDS166..5016
    Translation

    Target ORF information:

    RefSeq Version XM_015281617.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281617.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGATG TACTGGAAGA AATACAGCTA CCGGTTATGA AAGTCCCTCA GAATCTGTAC 
    CAAAACAGGT TCCTGGGCCT CGCAGCCATG GCGTCTCCAT CTAGGAACTC ACAAAATCGG
    CGCCGGTGCA AAGAGCCACT CAGATACAGC TATAACCCTG ACCAGTTTCA TAACATGGAC
    ATCAGGAACA ATTCCCATGA GACAGTCACC ATTCCTAGGT CTACCAGTGA CACGGACCTT
    GTCACTTCAG ACAGCCGATC TACCCTGATG GTCAGCAGCT CCTACTATTC CATTGGGCAC
    TCACAGGACC TAGTGATATA CTGGGATATA AAGGAGGAAG TGGATGCAGG GGACTGGATT
    GGCATGTATC TCATAGATGA AGTCTTGTCT GAAAACTTTT TGGACTACAA GAACCGTGGA
    GTCAATGGCT CTCATCGTGG TCAGATTGTC TGGAAGATTG ATGCCAGTTC TTACTTTGTG
    GAGCCTGAAA CAAAGATATG CTTCAAATAC TATCACGGTG TGAGTGGGGC ACTACGAGCC
    ACAACTCCAA GCGTCACGGT GAAAAACAAT GCAGCTCCTC AGATTTTTAA AAGCATCACC
    AATGAAGATT CAATCCAGGG ACAGGGAAAT CGGAGGCTGA TAAGTTTTTC ACTGTCAGAT
    TTCCAAGCTT TTGGTCTGAA GAAAGGAATG TTTTTCAATC CAGATCCTTA TCTGAAGATT
    TCCATCCAGC CTGGGAAACA CAGCATCTTC CCAGCACTTC CTCATCATGG ACAGGAGAAA
    AGGTCTAAGA TCATGTGTAA TACAGTTAAC CCAATCTGGC AAGGAGAGCA ATTCAGCTTT
    GTCTCACTGC CCACGGATGT CCTGGAAATA GAAGTTAAAG ACAAATTTGC AAAGAGCCGA
    CCAATCATCA AGCGTTTCCT GGGAAAACTC TCTATGCCAG TTCAGCGTCT CTTGGAAAGA
    CATGCCATAG GGGACAGGGT TGTAAGCTAC ACTCTTGGTC GCAGGCTTCC TACAGATCAT
    GTCAGTGGCC AGCTGCAGTT CCGATTTGAG ATAACTTCTT CCATTCATCA AGATGATGAA
    GAGGTTACCA TTAGTGCAGA TCCTGAGCCA GCTGACCTCC AGGAAAGCCC TGTGAACAAC
    CCCACAGAGG AAAGCCTCGG TGAGCCTCCA TGCATCAACA CAGTGACCTC AGAAAATACA
    GTTCCTGAAG AGCTAAATGG ATACAGTGTG AACAATATTG ATAGCCCAGG GGCTGTCAAA
    CCACCCAGGG AAGTCAACCA GAACAACATA AATGAAGAGC TAGCAGGAGA TGAGGAGGTT
    GATGCCATGC CAGTTCCTGA ACCCTTGGAA TCCATCCGAG GAGAAGTGCT GCCTTCTTTG
    AATGCGACAG ACCTCACAGA GCAGCTGGGT CTGCTGGCTT GCTTGTCTCC TTCTGAGACT
    GAAGTTAGGG AAAATAGCAA AGTCAATTCT GGTACTCAGG GAGATGATGG AGTATTGAAC
    AGTACAGAAA CTTTAGATGT TAGTGAGGTG AGTGCAGATG TATATGCTGA CAAAGACCTA
    GAGAAGAGTC ATGAAGAAGA AGGGGCTTCA GCCCGAGGGG AGGAAGACAG CAAGCTACAG
    CTGAGGACCA CAGCAAAGAG GAAAACAAGG CCATGCTCCC TGCCTGTGTC TGAAATTGAG
    ACCGTTATAG CTTCAGCTTG TGGTGAGCCA GAGACCCCTC GCACTCACTA TATACGAATC
    CACAACCTGC TCCACAGCCT GCCCTCAGCA CAGATGAGCA GTGATGGGGA AGAAGAGCAA
    GATGCTGGCA ATGGTGGGGA GAATGATGAA GAGTCTACAG TCAAGGAAGT GTTGCAGAAG
    GAGACAGTCA GTGAAAGTGA GACAATGGCA GCAGAGCCTC CTCCAGAAAA TGAGGCCGAA
    GAGAACTTCA GCCAGTGCAC AGTGCCTCCT GGGACCTTAG ACGAGCAACT CTCTGACTTA
    AATGATGGAC TGGTGGATGG GGAAAACCCT AGGACAGGAA GCGAAAGCGA GTCAAGCTCC
    AGACCCAATG GTGACAACGA ATGTGAGGCG TGCGACACCT CTTGCTACAG CCCCTCCTGC
    TATAGTACCT CCTGCTACAG TACTTCCTGT TACAGCACCT CTTGCTACAG CACCTCCTGC
    TATGACAGTA ACAATCGCTT CTCCAGCCAT ACCCGCTTCT CCTCTGTTGA CAGTGCAAAG
    ATTTCTGAGA GCACGGTCTT TTCCTCACAG GATGAGGAGG AGGAGAATAG TGCTTTTGAA
    TCGGTGCCAG ACTCAGTACA GAGCCCTGAG CTGGACAGGG AGCAAGTGGA TGGCTCCTCC
    CAGTGGCCAG ATGAACTAGT AGCTCATGGT GGGAATCCAC AACTAGCCGC AGAAAGTCTA
    GACACCCCAA TAGCAGGTCC AAGCAGCAGA AGAGAAGGTG ATTGTCCTTT ACTCCATAAT
    TCTCAGCCAG TCAGCCAACT TCCTTCTCTG AGGCCTGACC ATCATCACTA CCCAACTATC
    GATGAGCCTC TTCCACCAAA TTGGGAAGCT CGGATAGACA GCCACGGGAG GGTGTTCTAC
    GTTGACCATG TCAACCGAAC TACCACATGG CAGCGCCCCA CAGCTGCAGC AACACCAGAT
    GGGATCCACA GGTCTGGCTC TATCCAGCAA ATGGAGCAGC TGAACCGGCG ATATCAAAAT
    ATCCAGCGAA CTATTGCAAC AGAGCGGACT GAAGATGATG GTGTAGTAAA CAACAGAGTG
    GAGAGAATTC CCACTGGAGG AGGAAGTGAC TCTGAGGCAG AGCCTTCCCA ACCAAGCTCC
    GAAATTAGGA GGGAAGGTTC CCTTTCTCCT GTGAATTCTC AAAAAATCAC CTTATTGCTG
    CAGTCTCCAG CTGTGAAGTT TATCACCAAT CCTGAGTTCT TCACTGTGCT GCATGCAAAC
    TATAGTGCCT ATCGAGTCTT CACTAACAGT ACCTGCTTGA AGCATATGAT CCTGAAGATC
    CGTAGAGATG CTCGTAATTT TGAGCGCTAT CAGCACAACA GAGACCTTGT AAATTTTATC
    AACATGTTTG CTGACACTCG ACTAGAGCTT CCTCGTGGCT GGGAAATAAA AACTGACCAG
    CAGGGCAAGT CTTTCTTCGT TGACCACAAC AGTCGTGCTA CCACATTCAT AGATCCCAGG
    ATCCCACTGC AGAATGGTCG TCTCCCCAAT CACTTGACTC ACAGGCAACA TCTTCAGCGG
    CTTCGTAGCT ACAGTGCTGG AGAGGCTTCT GAAGTCTCTC GGAACAGAGG AGTTACTCTG
    TTGGCCAGAC CAGGCAATAG TCTTGTAACA GCTATTCGAA ACCAGCATCA TTATGAGACA
    TTACCTCTGG CTTACAATGA CAAGATTGTT GCATTTCTAC GCCAACCTAA CATTTTTGAG
    ATGCTGCAAG AGCGTCAGCC CAGTTTGGCC AGAAATCATG CACTCAGGGA GAAGATCCAT
    TATATTCGGA CAGAGGGTAC ACATGGGCTT GAAAAGTTGT CCTGTGATGC AGATTTAGTA
    ATTCTGCTGA GCCTTTTTGA AGAAGATATC ATGTCCTACA TACCACTCCA GGCCACTTTC
    CATCCTGGTT ACAGTTTCTC TCCTCGCTGC TCACCCTGCT CATCTCCCCA AAATTCTCCA
    GGACTGCAGC GAGCAAGTGC AAGAGCACCC TCTCCATACA GGAGAGATTT TGAAGCCAAA
    CTACGCAATT TCTATCGAAA ACTTGAAGCC AAAGGATATG GACAAGGTCC AGGTAAAATT
    AAGTTAATTA TACGACGTGA CCACTTACTG GAAGGCACCT TCAACCAGGT GATGGCATAT
    TCACGCAAGG AGCTCCAGCG AAACAAACTC TACATCACAT TTGTTGGCGA GGAAGGGCTG
    GACTACAGTG GCCCCTCTCG AGAATTCTTT TTCCTTCTGT CTCAGGAGCT CTTCAATCCA
    TATTACGGGC TGTTTGAGTA TTCAGCCAAT GACACTTACA CTGTACAGAT TAGCCCCATG
    TCTGCCTTTG TTGAAAATCA CCTGGAATGG TTCAGGTTCA GTGGTCGTAT TCTTGGCCTT
    GCTCTCATTC ATCAGTACCT TCTTGATGCT TTCTTCACAA GGCCGTTCTA CAAAGCACTA
    TTAAGGCTGC CATGTGATCT AAGTGACTTA GAGTACCTGG ATGAAGAATT CCATCAGAGC
    TTGCAGTGGA TGAAAGATAA CAACATCACA GATATCCTGG ATCTCACCTT CACAGTGAAT
    GAGGAGGTGT TTGGACAGGT GACAGAGAGA GAGTTGAAAT CTGGAGGGGC AAATACAGCA
    GTGACAGAAA AGAACAAAAA AGAGTACATT GAGAGAATGG TTAAATGGCG AGTGGAACGA
    GGAGTTGTTC AACAAACAGA GGCCCTGGTC CGTGGCTTCT ATGAAGTTGT AGACTCAAGG
    CTTGTCTCTG TTTTTGATGC CAGAGAACTG GAGCTGGTTA TAGCTGGCAC TGCAGAAATA
    GATCTCAATG ACTGGCGTAA CAACACGGAA TATCGTGGAG GTTACCACGA TGGACACATT
    GTAATACGGT GGTTCTGGGC AGCAGTGGAG AGGTTTAACA ATGAACAGAG ACTCCGGTTG
    TTGCAGTTTG TTACTGGAAC ATCAAGTGTG CCATATGAGG GCTTTGCAGC TCTCCGAGGA
    AGCAATGGGC TTCGGCGTTT CTGTATAGAG AAATGGGGGA AAATAACCTC CCTTCCGAGG
    GCACATACAT GTTTCAACCG CTTGGATCTT CCACCTTACC CCTCATATTC TATGCTGTAT
    GAAAAGCTGT TGACAGCTGT TGAAGAAACT AGTACCTTTG GACTTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa31693
    Clone ID Related Accession (Same CDS sequence) XM_015281619.1 , XM_015281619.2 , XM_015281620.1 , XM_015281620.2
    Accession Version XM_015281619.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4851bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCTC CATCTAGGAA CTCACAAAAT CGGCGCCGGT GCAAAGAGCC ACTCAGATAC 
    AGCTATAACC CTGACCAGTT TCATAACATG GACATCAGGA ACAATTCCCA TGAGACAGTC
    ACCATTCCTA GGTCTACCAG TGACACGGAC CTTGTCACTT CAGACAGCCG ATCTACCCTG
    ATGGTCAGCA GCTCCTACTA TTCCATTGGG CACTCACAGG ACCTAGTGAT ATACTGGGAT
    ATAAAGGAGG AAGTGGATGC AGGGGACTGG ATTGGCATGT ATCTCATAGA TGAAGTCTTG
    TCTGAAAACT TTTTGGACTA CAAGAACCGT GGAGTCAATG GCTCTCATCG TGGTCAGATT
    GTCTGGAAGA TTGATGCCAG TTCTTACTTT GTGGAGCCTG AAACAAAGAT ATGCTTCAAA
    TACTATCACG GTGTGAGTGG GGCACTACGA GCCACAACTC CAAGCGTCAC GGTGAAAAAC
    AATGCAGCTC CTCAGATTTT TAAAAGCATC ACCAATGAAG ATTCAATCCA GGGACAGGGA
    AATCGGAGGC TGATAAGTTT TTCACTGTCA GATTTCCAAG CTTTTGGTCT GAAGAAAGGA
    ATGTTTTTCA ATCCAGATCC TTATCTGAAG ATTTCCATCC AGCCTGGGAA ACACAGCATC
    TTCCCAGCAC TTCCTCATCA TGGACAGGAG AAAAGGTCTA AGATCATGTG TAATACAGTT
    AACCCAATCT GGCAAGGAGA GCAATTCAGC TTTGTCTCAC TGCCCACGGA TGTCCTGGAA
    ATAGAAGTTA AAGACAAATT TGCAAAGAGC CGACCAATCA TCAAGCGTTT CCTGGGAAAA
    CTCTCTATGC CAGTTCAGCG TCTCTTGGAA AGACATGCCA TAGGGGACAG GGTTGTAAGC
    TACACTCTTG GTCGCAGGCT TCCTACAGAT CATGTCAGTG GCCAGCTGCA GTTCCGATTT
    GAGATAACTT CTTCCATTCA TCAAGATGAT GAAGAGGTTA CCATTAGTGC AGATCCTGAG
    CCAGCTGACC TCCAGGAAAG CCCTGTGAAC AACCCCACAG AGGAAAGCCT CGGTGAGCCT
    CCATGCATCA ACACAGTGAC CTCAGAAAAT ACAGTTCCTG AAGAGCTAAA TGGATACAGT
    GTGAACAATA TTGATAGCCC AGGGGCTGTC AAACCACCCA GGGAAGTCAA CCAGAACAAC
    ATAAATGAAG AGCTAGCAGG AGATGAGGAG GTTGATGCCA TGCCAGTTCC TGAACCCTTG
    GAATCCATCC GAGGAGAAGT GCTGCCTTCT TTGAATGCGA CAGACCTCAC AGAGCAGCTG
    GGTCTGCTGG CTTGCTTGTC TCCTTCTGAG ACTGAAGTTA GGGAAAATAG CAAAGTCAAT
    TCTGGTACTC AGGGAGATGA TGGAGTATTG AACAGTACAG AAACTTTAGA TGTTAGTGAG
    GTGAGTGCAG ATGTATATGC TGACAAAGAC CTAGAGAAGA GTCATGAAGA AGAAGGGGCT
    TCAGCCCGAG GGGAGGAAGA CAGCAAGCTA CAGCTGAGGA CCACAGCAAA GAGGAAAACA
    AGGCCATGCT CCCTGCCTGT GTCTGAAATT GAGACCGTTA TAGCTTCAGC TTGTGGTGAG
    CCAGAGACCC CTCGCACTCA CTATATACGA ATCCACAACC TGCTCCACAG CCTGCCCTCA
    GCACAGATGA GCAGTGATGG GGAAGAAGAG CAAGATGCTG GCAATGGTGG GGAGAATGAT
    GAAGAGTCTA CAGTCAAGGA AGTGTTGCAG AAGGAGACAG TCAGTGAAAG TGAGACAATG
    GCAGCAGAGC CTCCTCCAGA AAATGAGGCC GAAGAGAACT TCAGCCAGTG CACAGTGCCT
    CCTGGGACCT TAGACGAGCA ACTCTCTGAC TTAAATGATG GACTGGTGGA TGGGGAAAAC
    CCTAGGACAG GAAGCGAAAG CGAGTCAAGC TCCAGACCCA ATGGTGACAA CGAATGTGAG
    GCGTGCGACA CCTCTTGCTA CAGCCCCTCC TGCTATAGTA CCTCCTGCTA CAGTACTTCC
    TGTTACAGCA CCTCTTGCTA CAGCACCTCC TGCTATGACA GTAACAATCG CTTCTCCAGC
    CATACCCGCT TCTCCTCTGT TGACAGTGCA AAGATTTCTG AGAGCACGGT CTTTTCCTCA
    CAGGATGAGG AGGAGGAGAA TAGTGCTTTT GAATCGGTGC CAGACTCAGT ACAGAGCCCT
    GAGCTGGACA GGGAGCAAGT GGATGGCTCC TCCCAGTGGC CAGATGAACT AGTAGCTCAT
    GGTGGGAATC CACAACTAGC CGCAGAAAGT CTAGACACCC CAATAGCAGG TCCAAGCAGC
    AGAAGAGAAG GTGATTGTCC TTTACTCCAT AATTCTCAGC CAGTCAGCCA ACTTCCTTCT
    CTGAGGCCTG ACCATCATCA CTACCCAACT ATCGATGAGC CTCTTCCACC AACTGCAACA
    TCCTTGAGAA ACCTTACCCA GTTGGATAAC CAGAGAGCCC TCCCTTTAAA ATTATGTGAT
    GTTCACAGAG AGACTGTATA TTGGGAAGCT CGGATAGACA GCCACGGGAG GGTGTTCTAC
    GTTGACCATG TCAACCGAAC TACCACATGG CAGCGCCCCA CAGCTGCAGC AACACCAGAT
    GGGATCCACA GGTCTGGCTC TATCCAGCAA ATGGAGCAGC TGAACCGGCG ATATCAAAAT
    ATCCAGCGAA CTATTGCAAC AGAGCGGACT GAAGATGATG GTGTAGTAAA CAACAGAGTG
    GAGAGAATTC CCACTGGAGG AGGAAGTGAC TCTGAGGCAG AGCCTTCCCA ACCAAGCTCC
    GAAATTAGGA GGGAAGGTTC CCTTTCTCCT GTGAATTCTC AAAAAATCAC CTTATTGCTG
    CAGTCTCCAG CTGTGAAGTT TATCACCAAT CCTGAGTTCT TCACTGTGCT GCATGCAAAC
    TATAGTGCCT ATCGAGTCTT CACTAACAGT ACCTGCTTGA AGCATATGAT CCTGAAGATC
    CGTAGAGATG CTCGTAATTT TGAGCGCTAT CAGCACAACA GAGACCTTGT AAATTTTATC
    AACATGTTTG CTGACACTCG ACTAGAGCTT CCTCGTGGCT GGGAAATAAA AACTGACCAG
    CAGGGCAAGT CTTTCTTCGT TGACCACAAC AGTCGTGCTA CCACATTCAT AGATCCCAGG
    ATCCCACTGC AGAATGGTCG TCTCCCCAAT CACTTGACTC ACAGGCAACA TCTTCAGCGG
    CTTCGTAGCT ACAGTGCTGG AGAGGCTTCT GAAGTCTCTC GGAACAGAGG AGTTACTCTG
    TTGGCCAGAC CAGGCAATAG TCTTGTAACA GCTATTCGAA ACCAGCATCA TTATGAGACA
    TTACCTCTGG CTTACAATGA CAAGATTGTT GCATTTCTAC GCCAACCTAA CATTTTTGAG
    ATGCTGCAAG AGCGTCAGCC CAGTTTGGCC AGAAATCATG CACTCAGGGA GAAGATCCAT
    TATATTCGGA CAGAGGGTAC ACATGGGCTT GAAAAGTTGT CCTGTGATGC AGATTTAGTA
    ATTCTGCTGA GCCTTTTTGA AGAAGATATC ATGTCCTACA TACCACTCCA GGCCACTTTC
    CATCCTGGTT ACAGTTTCTC TCCTCGCTGC TCACCCTGCT CATCTCCCCA AAATTCTCCA
    GGACTGCAGC GAGCAAGTGC AAGAGCACCC TCTCCATACA GGAGAGATTT TGAAGCCAAA
    CTACGCAATT TCTATCGAAA ACTTGAAGCC AAAGGATATG GACAAGGTCC AGGTAAAATT
    AAGTTAATTA TACGACGTGA CCACTTACTG GAAGGCACCT TCAACCAGGT GATGGCATAT
    TCACGCAAGG AGCTCCAGCG AAACAAACTC TACATCACAT TTGTTGGCGA GGAAGGGCTG
    GACTACAGTG GCCCCTCTCG AGAATTCTTT TTCCTTCTGT CTCAGGAGCT CTTCAATCCA
    TATTACGGGC TGTTTGAGTA TTCAGCCAAT GACACTTACA CTGTACAGAT TAGCCCCATG
    TCTGCCTTTG TTGAAAATCA CCTGGAATGG TTCAGGTTCA GTGGTCGTAT TCTTGGCCTT
    GCTCTCATTC ATCAGTACCT TCTTGATGCT TTCTTCACAA GGCCGTTCTA CAAAGCACTA
    TTAAGGCTGC CATGTGATCT AAGTGACTTA GAGTACCTGG ATGAAGAATT CCATCAGAGC
    TTGCAGTGGA TGAAAGATAA CAACATCACA GATATCCTGG ATCTCACCTT CACAGTGAAT
    GAGGAGGTGT TTGGACAGGT GACAGAGAGA GAGTTGAAAT CTGGAGGGGC AAATACAGCA
    GTGACAGAAA AGAACAAAAA AGAGTACATT GAGAGAATGG TTAAATGGCG AGTGGAACGA
    GGAGTTGTTC AACAAACAGA GGCCCTGGTC CGTGGCTTCT ATGAAGTTGT AGACTCAAGG
    CTTGTCTCTG TTTTTGATGC CAGAGAACTG GAGCTGGTTA TAGCTGGCAC TGCAGAAATA
    GATCTCAATG ACTGGCGTAA CAACACGGAA TATCGTGGAG GTTACCACGA TGGACACATT
    GTAATACGGT GGTTCTGGGC AGCAGTGGAG AGGTTTAACA ATGAACAGAG ACTCCGGTTG
    TTGCAGTTTG TTACTGGAAC ATCAAGTGTG CCATATGAGG GCTTTGCAGC TCTCCGAGGA
    AGCAATGGGC TTCGGCGTTT CTGTATAGAG AAATGGGGGA AAATAACCTC CCTTCCGAGG
    GCACATACAT GTTTCAACCG CTTGGATCTT CCACCTTACC CCTCATATTC TATGCTGTAT
    GAAAAGCTGT TGACAGCTGT TGAAGAAACT AGTACCTTTG GACTTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137105.1
    CDS271..5121
    Translation

    Target ORF information:

    RefSeq Version XM_015281619.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281619.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCTC CATCTAGGAA CTCACAAAAT CGGCGCCGGT GCAAAGAGCC ACTCAGATAC 
    AGCTATAACC CTGACCAGTT TCATAACATG GACATCAGGA ACAATTCCCA TGAGACAGTC
    ACCATTCCTA GGTCTACCAG TGACACGGAC CTTGTCACTT CAGACAGCCG ATCTACCCTG
    ATGGTCAGCA GCTCCTACTA TTCCATTGGG CACTCACAGG ACCTAGTGAT ATACTGGGAT
    ATAAAGGAGG AAGTGGATGC AGGGGACTGG ATTGGCATGT ATCTCATAGA TGAAGTCTTG
    TCTGAAAACT TTTTGGACTA CAAGAACCGT GGAGTCAATG GCTCTCATCG TGGTCAGATT
    GTCTGGAAGA TTGATGCCAG TTCTTACTTT GTGGAGCCTG AAACAAAGAT ATGCTTCAAA
    TACTATCACG GTGTGAGTGG GGCACTACGA GCCACAACTC CAAGCGTCAC GGTGAAAAAC
    AATGCAGCTC CTCAGATTTT TAAAAGCATC ACCAATGAAG ATTCAATCCA GGGACAGGGA
    AATCGGAGGC TGATAAGTTT TTCACTGTCA GATTTCCAAG CTTTTGGTCT GAAGAAAGGA
    ATGTTTTTCA ATCCAGATCC TTATCTGAAG ATTTCCATCC AGCCTGGGAA ACACAGCATC
    TTCCCAGCAC TTCCTCATCA TGGACAGGAG AAAAGGTCTA AGATCATGTG TAATACAGTT
    AACCCAATCT GGCAAGGAGA GCAATTCAGC TTTGTCTCAC TGCCCACGGA TGTCCTGGAA
    ATAGAAGTTA AAGACAAATT TGCAAAGAGC CGACCAATCA TCAAGCGTTT CCTGGGAAAA
    CTCTCTATGC CAGTTCAGCG TCTCTTGGAA AGACATGCCA TAGGGGACAG GGTTGTAAGC
    TACACTCTTG GTCGCAGGCT TCCTACAGAT CATGTCAGTG GCCAGCTGCA GTTCCGATTT
    GAGATAACTT CTTCCATTCA TCAAGATGAT GAAGAGGTTA CCATTAGTGC AGATCCTGAG
    CCAGCTGACC TCCAGGAAAG CCCTGTGAAC AACCCCACAG AGGAAAGCCT CGGTGAGCCT
    CCATGCATCA ACACAGTGAC CTCAGAAAAT ACAGTTCCTG AAGAGCTAAA TGGATACAGT
    GTGAACAATA TTGATAGCCC AGGGGCTGTC AAACCACCCA GGGAAGTCAA CCAGAACAAC
    ATAAATGAAG AGCTAGCAGG AGATGAGGAG GTTGATGCCA TGCCAGTTCC TGAACCCTTG
    GAATCCATCC GAGGAGAAGT GCTGCCTTCT TTGAATGCGA CAGACCTCAC AGAGCAGCTG
    GGTCTGCTGG CTTGCTTGTC TCCTTCTGAG ACTGAAGTTA GGGAAAATAG CAAAGTCAAT
    TCTGGTACTC AGGGAGATGA TGGAGTATTG AACAGTACAG AAACTTTAGA TGTTAGTGAG
    GTGAGTGCAG ATGTATATGC TGACAAAGAC CTAGAGAAGA GTCATGAAGA AGAAGGGGCT
    TCAGCCCGAG GGGAGGAAGA CAGCAAGCTA CAGCTGAGGA CCACAGCAAA GAGGAAAACA
    AGGCCATGCT CCCTGCCTGT GTCTGAAATT GAGACCGTTA TAGCTTCAGC TTGTGGTGAG
    CCAGAGACCC CTCGCACTCA CTATATACGA ATCCACAACC TGCTCCACAG CCTGCCCTCA
    GCACAGATGA GCAGTGATGG GGAAGAAGAG CAAGATGCTG GCAATGGTGG GGAGAATGAT
    GAAGAGTCTA CAGTCAAGGA AGTGTTGCAG AAGGAGACAG TCAGTGAAAG TGAGACAATG
    GCAGCAGAGC CTCCTCCAGA AAATGAGGCC GAAGAGAACT TCAGCCAGTG CACAGTGCCT
    CCTGGGACCT TAGACGAGCA ACTCTCTGAC TTAAATGATG GACTGGTGGA TGGGGAAAAC
    CCTAGGACAG GAAGCGAAAG CGAGTCAAGC TCCAGACCCA ATGGTGACAA CGAATGTGAG
    GCGTGCGACA CCTCTTGCTA CAGCCCCTCC TGCTATAGTA CCTCCTGCTA CAGTACTTCC
    TGTTACAGCA CCTCTTGCTA CAGCACCTCC TGCTATGACA GTAACAATCG CTTCTCCAGC
    CATACCCGCT TCTCCTCTGT TGACAGTGCA AAGATTTCTG AGAGCACGGT CTTTTCCTCA
    CAGGATGAGG AGGAGGAGAA TAGTGCTTTT GAATCGGTGC CAGACTCAGT ACAGAGCCCT
    GAGCTGGACA GGGAGCAAGT GGATGGCTCC TCCCAGTGGC CAGATGAACT AGTAGCTCAT
    GGTGGGAATC CACAACTAGC CGCAGAAAGT CTAGACACCC CAATAGCAGG TCCAAGCAGC
    AGAAGAGAAG GTGATTGTCC TTTACTCCAT AATTCTCAGC CAGTCAGCCA ACTTCCTTCT
    CTGAGGCCTG ACCATCATCA CTACCCAACT ATCGATGAGC CTCTTCCACC AACTGCAACA
    TCCTTGAGAA ACCTTACCCA GTTGGATAAC CAGAGAGCCC TCCCTTTAAA ATTATGTGAT
    GTTCACAGAG AGACTGTATA TTGGGAAGCT CGGATAGACA GCCACGGGAG GGTGTTCTAC
    GTTGACCATG TCAACCGAAC TACCACATGG CAGCGCCCCA CAGCTGCAGC AACACCAGAT
    GGGATCCACA GGTCTGGCTC TATCCAGCAA ATGGAGCAGC TGAACCGGCG ATATCAAAAT
    ATCCAGCGAA CTATTGCAAC AGAGCGGACT GAAGATGATG GTGTAGTAAA CAACAGAGTG
    GAGAGAATTC CCACTGGAGG AGGAAGTGAC TCTGAGGCAG AGCCTTCCCA ACCAAGCTCC
    GAAATTAGGA GGGAAGGTTC CCTTTCTCCT GTGAATTCTC AAAAAATCAC CTTATTGCTG
    CAGTCTCCAG CTGTGAAGTT TATCACCAAT CCTGAGTTCT TCACTGTGCT GCATGCAAAC
    TATAGTGCCT ATCGAGTCTT CACTAACAGT ACCTGCTTGA AGCATATGAT CCTGAAGATC
    CGTAGAGATG CTCGTAATTT TGAGCGCTAT CAGCACAACA GAGACCTTGT AAATTTTATC
    AACATGTTTG CTGACACTCG ACTAGAGCTT CCTCGTGGCT GGGAAATAAA AACTGACCAG
    CAGGGCAAGT CTTTCTTCGT TGACCACAAC AGTCGTGCTA CCACATTCAT AGATCCCAGG
    ATCCCACTGC AGAATGGTCG TCTCCCCAAT CACTTGACTC ACAGGCAACA TCTTCAGCGG
    CTTCGTAGCT ACAGTGCTGG AGAGGCTTCT GAAGTCTCTC GGAACAGAGG AGTTACTCTG
    TTGGCCAGAC CAGGCAATAG TCTTGTAACA GCTATTCGAA ACCAGCATCA TTATGAGACA
    TTACCTCTGG CTTACAATGA CAAGATTGTT GCATTTCTAC GCCAACCTAA CATTTTTGAG
    ATGCTGCAAG AGCGTCAGCC CAGTTTGGCC AGAAATCATG CACTCAGGGA GAAGATCCAT
    TATATTCGGA CAGAGGGTAC ACATGGGCTT GAAAAGTTGT CCTGTGATGC AGATTTAGTA
    ATTCTGCTGA GCCTTTTTGA AGAAGATATC ATGTCCTACA TACCACTCCA GGCCACTTTC
    CATCCTGGTT ACAGTTTCTC TCCTCGCTGC TCACCCTGCT CATCTCCCCA AAATTCTCCA
    GGACTGCAGC GAGCAAGTGC AAGAGCACCC TCTCCATACA GGAGAGATTT TGAAGCCAAA
    CTACGCAATT TCTATCGAAA ACTTGAAGCC AAAGGATATG GACAAGGTCC AGGTAAAATT
    AAGTTAATTA TACGACGTGA CCACTTACTG GAAGGCACCT TCAACCAGGT GATGGCATAT
    TCACGCAAGG AGCTCCAGCG AAACAAACTC TACATCACAT TTGTTGGCGA GGAAGGGCTG
    GACTACAGTG GCCCCTCTCG AGAATTCTTT TTCCTTCTGT CTCAGGAGCT CTTCAATCCA
    TATTACGGGC TGTTTGAGTA TTCAGCCAAT GACACTTACA CTGTACAGAT TAGCCCCATG
    TCTGCCTTTG TTGAAAATCA CCTGGAATGG TTCAGGTTCA GTGGTCGTAT TCTTGGCCTT
    GCTCTCATTC ATCAGTACCT TCTTGATGCT TTCTTCACAA GGCCGTTCTA CAAAGCACTA
    TTAAGGCTGC CATGTGATCT AAGTGACTTA GAGTACCTGG ATGAAGAATT CCATCAGAGC
    TTGCAGTGGA TGAAAGATAA CAACATCACA GATATCCTGG ATCTCACCTT CACAGTGAAT
    GAGGAGGTGT TTGGACAGGT GACAGAGAGA GAGTTGAAAT CTGGAGGGGC AAATACAGCA
    GTGACAGAAA AGAACAAAAA AGAGTACATT GAGAGAATGG TTAAATGGCG AGTGGAACGA
    GGAGTTGTTC AACAAACAGA GGCCCTGGTC CGTGGCTTCT ATGAAGTTGT AGACTCAAGG
    CTTGTCTCTG TTTTTGATGC CAGAGAACTG GAGCTGGTTA TAGCTGGCAC TGCAGAAATA
    GATCTCAATG ACTGGCGTAA CAACACGGAA TATCGTGGAG GTTACCACGA TGGACACATT
    GTAATACGGT GGTTCTGGGC AGCAGTGGAG AGGTTTAACA ATGAACAGAG ACTCCGGTTG
    TTGCAGTTTG TTACTGGAAC ATCAAGTGTG CCATATGAGG GCTTTGCAGC TCTCCGAGGA
    AGCAATGGGC TTCGGCGTTT CTGTATAGAG AAATGGGGGA AAATAACCTC CCTTCCGAGG
    GCACATACAT GTTTCAACCG CTTGGATCTT CCACCTTACC CCTCATATTC TATGCTGTAT
    GAAAAGCTGT TGACAGCTGT TGAAGAAACT AGTACCTTTG GACTTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa31693
    Clone ID Related Accession (Same CDS sequence) XM_015281619.1 , XM_015281619.2 , XM_015281620.1 , XM_015281620.2
    Accession Version XM_015281619.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4851bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281619.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCTC CATCTAGGAA CTCACAAAAT CGGCGCCGGT GCAAAGAGCC ACTCAGATAC 
    AGCTATAACC CTGACCAGTT TCATAACATG GACATCAGGA ACAATTCCCA TGAGACAGTC
    ACCATTCCTA GGTCTACCAG TGACACGGAC CTTGTCACTT CAGACAGCCG ATCTACCCTG
    ATGGTCAGCA GCTCCTACTA TTCCATTGGG CACTCACAGG ACCTAGTGAT ATACTGGGAT
    ATAAAGGAGG AAGTGGATGC AGGGGACTGG ATTGGCATGT ATCTCATAGA TGAAGTCTTG
    TCTGAAAACT TTTTGGACTA CAAGAACCGT GGAGTCAATG GCTCTCATCG TGGTCAGATT
    GTCTGGAAGA TTGATGCCAG TTCTTACTTT GTGGAGCCTG AAACAAAGAT ATGCTTCAAA
    TACTATCACG GTGTGAGTGG GGCACTACGA GCCACAACTC CAAGCGTCAC GGTGAAAAAC
    AATGCAGCTC CTCAGATTTT TAAAAGCATC ACCAATGAAG ATTCAATCCA GGGACAGGGA
    AATCGGAGGC TGATAAGTTT TTCACTGTCA GATTTCCAAG CTTTTGGTCT GAAGAAAGGA
    ATGTTTTTCA ATCCAGATCC TTATCTGAAG ATTTCCATCC AGCCTGGGAA ACACAGCATC
    TTCCCAGCAC TTCCTCATCA TGGACAGGAG AAAAGGTCTA AGATCATGTG TAATACAGTT
    AACCCAATCT GGCAAGGAGA GCAATTCAGC TTTGTCTCAC TGCCCACGGA TGTCCTGGAA
    ATAGAAGTTA AAGACAAATT TGCAAAGAGC CGACCAATCA TCAAGCGTTT CCTGGGAAAA
    CTCTCTATGC CAGTTCAGCG TCTCTTGGAA AGACATGCCA TAGGGGACAG GGTTGTAAGC
    TACACTCTTG GTCGCAGGCT TCCTACAGAT CATGTCAGTG GCCAGCTGCA GTTCCGATTT
    GAGATAACTT CTTCCATTCA TCAAGATGAT GAAGAGGTTA CCATTAGTGC AGATCCTGAG
    CCAGCTGACC TCCAGGAAAG CCCTGTGAAC AACCCCACAG AGGAAAGCCT CGGTGAGCCT
    CCATGCATCA ACACAGTGAC CTCAGAAAAT ACAGTTCCTG AAGAGCTAAA TGGATACAGT
    GTGAACAATA TTGATAGCCC AGGGGCTGTC AAACCACCCA GGGAAGTCAA CCAGAACAAC
    ATAAATGAAG AGCTAGCAGG AGATGAGGAG GTTGATGCCA TGCCAGTTCC TGAACCCTTG
    GAATCCATCC GAGGAGAAGT GCTGCCTTCT TTGAATGCGA CAGACCTCAC AGAGCAGCTG
    GGTCTGCTGG CTTGCTTGTC TCCTTCTGAG ACTGAAGTTA GGGAAAATAG CAAAGTCAAT
    TCTGGTACTC AGGGAGATGA TGGAGTATTG AACAGTACAG AAACTTTAGA TGTTAGTGAG
    GTGAGTGCAG ATGTATATGC TGACAAAGAC CTAGAGAAGA GTCATGAAGA AGAAGGGGCT
    TCAGCCCGAG GGGAGGAAGA CAGCAAGCTA CAGCTGAGGA CCACAGCAAA GAGGAAAACA
    AGGCCATGCT CCCTGCCTGT GTCTGAAATT GAGACCGTTA TAGCTTCAGC TTGTGGTGAG
    CCAGAGACCC CTCGCACTCA CTATATACGA ATCCACAACC TGCTCCACAG CCTGCCCTCA
    GCACAGATGA GCAGTGATGG GGAAGAAGAG CAAGATGCTG GCAATGGTGG GGAGAATGAT
    GAAGAGTCTA CAGTCAAGGA AGTGTTGCAG AAGGAGACAG TCAGTGAAAG TGAGACAATG
    GCAGCAGAGC CTCCTCCAGA AAATGAGGCC GAAGAGAACT TCAGCCAGTG CACAGTGCCT
    CCTGGGACCT TAGACGAGCA ACTCTCTGAC TTAAATGATG GACTGGTGGA TGGGGAAAAC
    CCTAGGACAG GAAGCGAAAG CGAGTCAAGC TCCAGACCCA ATGGTGACAA CGAATGTGAG
    GCGTGCGACA CCTCTTGCTA CAGCCCCTCC TGCTATAGTA CCTCCTGCTA CAGTACTTCC
    TGTTACAGCA CCTCTTGCTA CAGCACCTCC TGCTATGACA GTAACAATCG CTTCTCCAGC
    CATACCCGCT TCTCCTCTGT TGACAGTGCA AAGATTTCTG AGAGCACGGT CTTTTCCTCA
    CAGGATGAGG AGGAGGAGAA TAGTGCTTTT GAATCGGTGC CAGACTCAGT ACAGAGCCCT
    GAGCTGGACA GGGAGCAAGT GGATGGCTCC TCCCAGTGGC CAGATGAACT AGTAGCTCAT
    GGTGGGAATC CACAACTAGC CGCAGAAAGT CTAGACACCC CAATAGCAGG TCCAAGCAGC
    AGAAGAGAAG GTGATTGTCC TTTACTCCAT AATTCTCAGC CAGTCAGCCA ACTTCCTTCT
    CTGAGGCCTG ACCATCATCA CTACCCAACT ATCGATGAGC CTCTTCCACC AACTGCAACA
    TCCTTGAGAA ACCTTACCCA GTTGGATAAC CAGAGAGCCC TCCCTTTAAA ATTATGTGAT
    GTTCACAGAG AGACTGTATA TTGGGAAGCT CGGATAGACA GCCACGGGAG GGTGTTCTAC
    GTTGACCATG TCAACCGAAC TACCACATGG CAGCGCCCCA CAGCTGCAGC AACACCAGAT
    GGGATCCACA GGTCTGGCTC TATCCAGCAA ATGGAGCAGC TGAACCGGCG ATATCAAAAT
    ATCCAGCGAA CTATTGCAAC AGAGCGGACT GAAGATGATG GTGTAGTAAA CAACAGAGTG
    GAGAGAATTC CCACTGGAGG AGGAAGTGAC TCTGAGGCAG AGCCTTCCCA ACCAAGCTCC
    GAAATTAGGA GGGAAGGTTC CCTTTCTCCT GTGAATTCTC AAAAAATCAC CTTATTGCTG
    CAGTCTCCAG CTGTGAAGTT TATCACCAAT CCTGAGTTCT TCACTGTGCT GCATGCAAAC
    TATAGTGCCT ATCGAGTCTT CACTAACAGT ACCTGCTTGA AGCATATGAT CCTGAAGATC
    CGTAGAGATG CTCGTAATTT TGAGCGCTAT CAGCACAACA GAGACCTTGT AAATTTTATC
    AACATGTTTG CTGACACTCG ACTAGAGCTT CCTCGTGGCT GGGAAATAAA AACTGACCAG
    CAGGGCAAGT CTTTCTTCGT TGACCACAAC AGTCGTGCTA CCACATTCAT AGATCCCAGG
    ATCCCACTGC AGAATGGTCG TCTCCCCAAT CACTTGACTC ACAGGCAACA TCTTCAGCGG
    CTTCGTAGCT ACAGTGCTGG AGAGGCTTCT GAAGTCTCTC GGAACAGAGG AGTTACTCTG
    TTGGCCAGAC CAGGCAATAG TCTTGTAACA GCTATTCGAA ACCAGCATCA TTATGAGACA
    TTACCTCTGG CTTACAATGA CAAGATTGTT GCATTTCTAC GCCAACCTAA CATTTTTGAG
    ATGCTGCAAG AGCGTCAGCC CAGTTTGGCC AGAAATCATG CACTCAGGGA GAAGATCCAT
    TATATTCGGA CAGAGGGTAC ACATGGGCTT GAAAAGTTGT CCTGTGATGC AGATTTAGTA
    ATTCTGCTGA GCCTTTTTGA AGAAGATATC ATGTCCTACA TACCACTCCA GGCCACTTTC
    CATCCTGGTT ACAGTTTCTC TCCTCGCTGC TCACCCTGCT CATCTCCCCA AAATTCTCCA
    GGACTGCAGC GAGCAAGTGC AAGAGCACCC TCTCCATACA GGAGAGATTT TGAAGCCAAA
    CTACGCAATT TCTATCGAAA ACTTGAAGCC AAAGGATATG GACAAGGTCC AGGTAAAATT
    AAGTTAATTA TACGACGTGA CCACTTACTG GAAGGCACCT TCAACCAGGT GATGGCATAT
    TCACGCAAGG AGCTCCAGCG AAACAAACTC TACATCACAT TTGTTGGCGA GGAAGGGCTG
    GACTACAGTG GCCCCTCTCG AGAATTCTTT TTCCTTCTGT CTCAGGAGCT CTTCAATCCA
    TATTACGGGC TGTTTGAGTA TTCAGCCAAT GACACTTACA CTGTACAGAT TAGCCCCATG
    TCTGCCTTTG TTGAAAATCA CCTGGAATGG TTCAGGTTCA GTGGTCGTAT TCTTGGCCTT
    GCTCTCATTC ATCAGTACCT TCTTGATGCT TTCTTCACAA GGCCGTTCTA CAAAGCACTA
    TTAAGGCTGC CATGTGATCT AAGTGACTTA GAGTACCTGG ATGAAGAATT CCATCAGAGC
    TTGCAGTGGA TGAAAGATAA CAACATCACA GATATCCTGG ATCTCACCTT CACAGTGAAT
    GAGGAGGTGT TTGGACAGGT GACAGAGAGA GAGTTGAAAT CTGGAGGGGC AAATACAGCA
    GTGACAGAAA AGAACAAAAA AGAGTACATT GAGAGAATGG TTAAATGGCG AGTGGAACGA
    GGAGTTGTTC AACAAACAGA GGCCCTGGTC CGTGGCTTCT ATGAAGTTGT AGACTCAAGG
    CTTGTCTCTG TTTTTGATGC CAGAGAACTG GAGCTGGTTA TAGCTGGCAC TGCAGAAATA
    GATCTCAATG ACTGGCGTAA CAACACGGAA TATCGTGGAG GTTACCACGA TGGACACATT
    GTAATACGGT GGTTCTGGGC AGCAGTGGAG AGGTTTAACA ATGAACAGAG ACTCCGGTTG
    TTGCAGTTTG TTACTGGAAC ATCAAGTGTG CCATATGAGG GCTTTGCAGC TCTCCGAGGA
    AGCAATGGGC TTCGGCGTTT CTGTATAGAG AAATGGGGGA AAATAACCTC CCTTCCGAGG
    GCACATACAT GTTTCAACCG CTTGGATCTT CCACCTTACC CCTCATATTC TATGCTGTAT
    GAAAAGCTGT TGACAGCTGT TGAAGAAACT AGTACCTTTG GACTTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137105.1
    CDS231..5081
    Translation

    Target ORF information:

    RefSeq Version XM_015281619.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281619.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCTC CATCTAGGAA CTCACAAAAT CGGCGCCGGT GCAAAGAGCC ACTCAGATAC 
    AGCTATAACC CTGACCAGTT TCATAACATG GACATCAGGA ACAATTCCCA TGAGACAGTC
    ACCATTCCTA GGTCTACCAG TGACACGGAC CTTGTCACTT CAGACAGCCG ATCTACCCTG
    ATGGTCAGCA GCTCCTACTA TTCCATTGGG CACTCACAGG ACCTAGTGAT ATACTGGGAT
    ATAAAGGAGG AAGTGGATGC AGGGGACTGG ATTGGCATGT ATCTCATAGA TGAAGTCTTG
    TCTGAAAACT TTTTGGACTA CAAGAACCGT GGAGTCAATG GCTCTCATCG TGGTCAGATT
    GTCTGGAAGA TTGATGCCAG TTCTTACTTT GTGGAGCCTG AAACAAAGAT ATGCTTCAAA
    TACTATCACG GTGTGAGTGG GGCACTACGA GCCACAACTC CAAGCGTCAC GGTGAAAAAC
    AATGCAGCTC CTCAGATTTT TAAAAGCATC ACCAATGAAG ATTCAATCCA GGGACAGGGA
    AATCGGAGGC TGATAAGTTT TTCACTGTCA GATTTCCAAG CTTTTGGTCT GAAGAAAGGA
    ATGTTTTTCA ATCCAGATCC TTATCTGAAG ATTTCCATCC AGCCTGGGAA ACACAGCATC
    TTCCCAGCAC TTCCTCATCA TGGACAGGAG AAAAGGTCTA AGATCATGTG TAATACAGTT
    AACCCAATCT GGCAAGGAGA GCAATTCAGC TTTGTCTCAC TGCCCACGGA TGTCCTGGAA
    ATAGAAGTTA AAGACAAATT TGCAAAGAGC CGACCAATCA TCAAGCGTTT CCTGGGAAAA
    CTCTCTATGC CAGTTCAGCG TCTCTTGGAA AGACATGCCA TAGGGGACAG GGTTGTAAGC
    TACACTCTTG GTCGCAGGCT TCCTACAGAT CATGTCAGTG GCCAGCTGCA GTTCCGATTT
    GAGATAACTT CTTCCATTCA TCAAGATGAT GAAGAGGTTA CCATTAGTGC AGATCCTGAG
    CCAGCTGACC TCCAGGAAAG CCCTGTGAAC AACCCCACAG AGGAAAGCCT CGGTGAGCCT
    CCATGCATCA ACACAGTGAC CTCAGAAAAT ACAGTTCCTG AAGAGCTAAA TGGATACAGT
    GTGAACAATA TTGATAGCCC AGGGGCTGTC AAACCACCCA GGGAAGTCAA CCAGAACAAC
    ATAAATGAAG AGCTAGCAGG AGATGAGGAG GTTGATGCCA TGCCAGTTCC TGAACCCTTG
    GAATCCATCC GAGGAGAAGT GCTGCCTTCT TTGAATGCGA CAGACCTCAC AGAGCAGCTG
    GGTCTGCTGG CTTGCTTGTC TCCTTCTGAG ACTGAAGTTA GGGAAAATAG CAAAGTCAAT
    TCTGGTACTC AGGGAGATGA TGGAGTATTG AACAGTACAG AAACTTTAGA TGTTAGTGAG
    GTGAGTGCAG ATGTATATGC TGACAAAGAC CTAGAGAAGA GTCATGAAGA AGAAGGGGCT
    TCAGCCCGAG GGGAGGAAGA CAGCAAGCTA CAGCTGAGGA CCACAGCAAA GAGGAAAACA
    AGGCCATGCT CCCTGCCTGT GTCTGAAATT GAGACCGTTA TAGCTTCAGC TTGTGGTGAG
    CCAGAGACCC CTCGCACTCA CTATATACGA ATCCACAACC TGCTCCACAG CCTGCCCTCA
    GCACAGATGA GCAGTGATGG GGAAGAAGAG CAAGATGCTG GCAATGGTGG GGAGAATGAT
    GAAGAGTCTA CAGTCAAGGA AGTGTTGCAG AAGGAGACAG TCAGTGAAAG TGAGACAATG
    GCAGCAGAGC CTCCTCCAGA AAATGAGGCC GAAGAGAACT TCAGCCAGTG CACAGTGCCT
    CCTGGGACCT TAGACGAGCA ACTCTCTGAC TTAAATGATG GACTGGTGGA TGGGGAAAAC
    CCTAGGACAG GAAGCGAAAG CGAGTCAAGC TCCAGACCCA ATGGTGACAA CGAATGTGAG
    GCGTGCGACA CCTCTTGCTA CAGCCCCTCC TGCTATAGTA CCTCCTGCTA CAGTACTTCC
    TGTTACAGCA CCTCTTGCTA CAGCACCTCC TGCTATGACA GTAACAATCG CTTCTCCAGC
    CATACCCGCT TCTCCTCTGT TGACAGTGCA AAGATTTCTG AGAGCACGGT CTTTTCCTCA
    CAGGATGAGG AGGAGGAGAA TAGTGCTTTT GAATCGGTGC CAGACTCAGT ACAGAGCCCT
    GAGCTGGACA GGGAGCAAGT GGATGGCTCC TCCCAGTGGC CAGATGAACT AGTAGCTCAT
    GGTGGGAATC CACAACTAGC CGCAGAAAGT CTAGACACCC CAATAGCAGG TCCAAGCAGC
    AGAAGAGAAG GTGATTGTCC TTTACTCCAT AATTCTCAGC CAGTCAGCCA ACTTCCTTCT
    CTGAGGCCTG ACCATCATCA CTACCCAACT ATCGATGAGC CTCTTCCACC AACTGCAACA
    TCCTTGAGAA ACCTTACCCA GTTGGATAAC CAGAGAGCCC TCCCTTTAAA ATTATGTGAT
    GTTCACAGAG AGACTGTATA TTGGGAAGCT CGGATAGACA GCCACGGGAG GGTGTTCTAC
    GTTGACCATG TCAACCGAAC TACCACATGG CAGCGCCCCA CAGCTGCAGC AACACCAGAT
    GGGATCCACA GGTCTGGCTC TATCCAGCAA ATGGAGCAGC TGAACCGGCG ATATCAAAAT
    ATCCAGCGAA CTATTGCAAC AGAGCGGACT GAAGATGATG GTGTAGTAAA CAACAGAGTG
    GAGAGAATTC CCACTGGAGG AGGAAGTGAC TCTGAGGCAG AGCCTTCCCA ACCAAGCTCC
    GAAATTAGGA GGGAAGGTTC CCTTTCTCCT GTGAATTCTC AAAAAATCAC CTTATTGCTG
    CAGTCTCCAG CTGTGAAGTT TATCACCAAT CCTGAGTTCT TCACTGTGCT GCATGCAAAC
    TATAGTGCCT ATCGAGTCTT CACTAACAGT ACCTGCTTGA AGCATATGAT CCTGAAGATC
    CGTAGAGATG CTCGTAATTT TGAGCGCTAT CAGCACAACA GAGACCTTGT AAATTTTATC
    AACATGTTTG CTGACACTCG ACTAGAGCTT CCTCGTGGCT GGGAAATAAA AACTGACCAG
    CAGGGCAAGT CTTTCTTCGT TGACCACAAC AGTCGTGCTA CCACATTCAT AGATCCCAGG
    ATCCCACTGC AGAATGGTCG TCTCCCCAAT CACTTGACTC ACAGGCAACA TCTTCAGCGG
    CTTCGTAGCT ACAGTGCTGG AGAGGCTTCT GAAGTCTCTC GGAACAGAGG AGTTACTCTG
    TTGGCCAGAC CAGGCAATAG TCTTGTAACA GCTATTCGAA ACCAGCATCA TTATGAGACA
    TTACCTCTGG CTTACAATGA CAAGATTGTT GCATTTCTAC GCCAACCTAA CATTTTTGAG
    ATGCTGCAAG AGCGTCAGCC CAGTTTGGCC AGAAATCATG CACTCAGGGA GAAGATCCAT
    TATATTCGGA CAGAGGGTAC ACATGGGCTT GAAAAGTTGT CCTGTGATGC AGATTTAGTA
    ATTCTGCTGA GCCTTTTTGA AGAAGATATC ATGTCCTACA TACCACTCCA GGCCACTTTC
    CATCCTGGTT ACAGTTTCTC TCCTCGCTGC TCACCCTGCT CATCTCCCCA AAATTCTCCA
    GGACTGCAGC GAGCAAGTGC AAGAGCACCC TCTCCATACA GGAGAGATTT TGAAGCCAAA
    CTACGCAATT TCTATCGAAA ACTTGAAGCC AAAGGATATG GACAAGGTCC AGGTAAAATT
    AAGTTAATTA TACGACGTGA CCACTTACTG GAAGGCACCT TCAACCAGGT GATGGCATAT
    TCACGCAAGG AGCTCCAGCG AAACAAACTC TACATCACAT TTGTTGGCGA GGAAGGGCTG
    GACTACAGTG GCCCCTCTCG AGAATTCTTT TTCCTTCTGT CTCAGGAGCT CTTCAATCCA
    TATTACGGGC TGTTTGAGTA TTCAGCCAAT GACACTTACA CTGTACAGAT TAGCCCCATG
    TCTGCCTTTG TTGAAAATCA CCTGGAATGG TTCAGGTTCA GTGGTCGTAT TCTTGGCCTT
    GCTCTCATTC ATCAGTACCT TCTTGATGCT TTCTTCACAA GGCCGTTCTA CAAAGCACTA
    TTAAGGCTGC CATGTGATCT AAGTGACTTA GAGTACCTGG ATGAAGAATT CCATCAGAGC
    TTGCAGTGGA TGAAAGATAA CAACATCACA GATATCCTGG ATCTCACCTT CACAGTGAAT
    GAGGAGGTGT TTGGACAGGT GACAGAGAGA GAGTTGAAAT CTGGAGGGGC AAATACAGCA
    GTGACAGAAA AGAACAAAAA AGAGTACATT GAGAGAATGG TTAAATGGCG AGTGGAACGA
    GGAGTTGTTC AACAAACAGA GGCCCTGGTC CGTGGCTTCT ATGAAGTTGT AGACTCAAGG
    CTTGTCTCTG TTTTTGATGC CAGAGAACTG GAGCTGGTTA TAGCTGGCAC TGCAGAAATA
    GATCTCAATG ACTGGCGTAA CAACACGGAA TATCGTGGAG GTTACCACGA TGGACACATT
    GTAATACGGT GGTTCTGGGC AGCAGTGGAG AGGTTTAACA ATGAACAGAG ACTCCGGTTG
    TTGCAGTTTG TTACTGGAAC ATCAAGTGTG CCATATGAGG GCTTTGCAGC TCTCCGAGGA
    AGCAATGGGC TTCGGCGTTT CTGTATAGAG AAATGGGGGA AAATAACCTC CCTTCCGAGG
    GCACATACAT GTTTCAACCG CTTGGATCTT CCACCTTACC CCTCATATTC TATGCTGTAT
    GAAAAGCTGT TGACAGCTGT TGAAGAAACT AGTACCTTTG GACTTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa31693
    Clone ID Related Accession (Same CDS sequence) XM_015281619.1 , XM_015281619.2 , XM_015281620.1 , XM_015281620.2
    Accession Version XM_015281620.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4851bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCTC CATCTAGGAA CTCACAAAAT CGGCGCCGGT GCAAAGAGCC ACTCAGATAC 
    AGCTATAACC CTGACCAGTT TCATAACATG GACATCAGGA ACAATTCCCA TGAGACAGTC
    ACCATTCCTA GGTCTACCAG TGACACGGAC CTTGTCACTT CAGACAGCCG ATCTACCCTG
    ATGGTCAGCA GCTCCTACTA TTCCATTGGG CACTCACAGG ACCTAGTGAT ATACTGGGAT
    ATAAAGGAGG AAGTGGATGC AGGGGACTGG ATTGGCATGT ATCTCATAGA TGAAGTCTTG
    TCTGAAAACT TTTTGGACTA CAAGAACCGT GGAGTCAATG GCTCTCATCG TGGTCAGATT
    GTCTGGAAGA TTGATGCCAG TTCTTACTTT GTGGAGCCTG AAACAAAGAT ATGCTTCAAA
    TACTATCACG GTGTGAGTGG GGCACTACGA GCCACAACTC CAAGCGTCAC GGTGAAAAAC
    AATGCAGCTC CTCAGATTTT TAAAAGCATC ACCAATGAAG ATTCAATCCA GGGACAGGGA
    AATCGGAGGC TGATAAGTTT TTCACTGTCA GATTTCCAAG CTTTTGGTCT GAAGAAAGGA
    ATGTTTTTCA ATCCAGATCC TTATCTGAAG ATTTCCATCC AGCCTGGGAA ACACAGCATC
    TTCCCAGCAC TTCCTCATCA TGGACAGGAG AAAAGGTCTA AGATCATGTG TAATACAGTT
    AACCCAATCT GGCAAGGAGA GCAATTCAGC TTTGTCTCAC TGCCCACGGA TGTCCTGGAA
    ATAGAAGTTA AAGACAAATT TGCAAAGAGC CGACCAATCA TCAAGCGTTT CCTGGGAAAA
    CTCTCTATGC CAGTTCAGCG TCTCTTGGAA AGACATGCCA TAGGGGACAG GGTTGTAAGC
    TACACTCTTG GTCGCAGGCT TCCTACAGAT CATGTCAGTG GCCAGCTGCA GTTCCGATTT
    GAGATAACTT CTTCCATTCA TCAAGATGAT GAAGAGGTTA CCATTAGTGC AGATCCTGAG
    CCAGCTGACC TCCAGGAAAG CCCTGTGAAC AACCCCACAG AGGAAAGCCT CGGTGAGCCT
    CCATGCATCA ACACAGTGAC CTCAGAAAAT ACAGTTCCTG AAGAGCTAAA TGGATACAGT
    GTGAACAATA TTGATAGCCC AGGGGCTGTC AAACCACCCA GGGAAGTCAA CCAGAACAAC
    ATAAATGAAG AGCTAGCAGG AGATGAGGAG GTTGATGCCA TGCCAGTTCC TGAACCCTTG
    GAATCCATCC GAGGAGAAGT GCTGCCTTCT TTGAATGCGA CAGACCTCAC AGAGCAGCTG
    GGTCTGCTGG CTTGCTTGTC TCCTTCTGAG ACTGAAGTTA GGGAAAATAG CAAAGTCAAT
    TCTGGTACTC AGGGAGATGA TGGAGTATTG AACAGTACAG AAACTTTAGA TGTTAGTGAG
    GTGAGTGCAG ATGTATATGC TGACAAAGAC CTAGAGAAGA GTCATGAAGA AGAAGGGGCT
    TCAGCCCGAG GGGAGGAAGA CAGCAAGCTA CAGCTGAGGA CCACAGCAAA GAGGAAAACA
    AGGCCATGCT CCCTGCCTGT GTCTGAAATT GAGACCGTTA TAGCTTCAGC TTGTGGTGAG
    CCAGAGACCC CTCGCACTCA CTATATACGA ATCCACAACC TGCTCCACAG CCTGCCCTCA
    GCACAGATGA GCAGTGATGG GGAAGAAGAG CAAGATGCTG GCAATGGTGG GGAGAATGAT
    GAAGAGTCTA CAGTCAAGGA AGTGTTGCAG AAGGAGACAG TCAGTGAAAG TGAGACAATG
    GCAGCAGAGC CTCCTCCAGA AAATGAGGCC GAAGAGAACT TCAGCCAGTG CACAGTGCCT
    CCTGGGACCT TAGACGAGCA ACTCTCTGAC TTAAATGATG GACTGGTGGA TGGGGAAAAC
    CCTAGGACAG GAAGCGAAAG CGAGTCAAGC TCCAGACCCA ATGGTGACAA CGAATGTGAG
    GCGTGCGACA CCTCTTGCTA CAGCCCCTCC TGCTATAGTA CCTCCTGCTA CAGTACTTCC
    TGTTACAGCA CCTCTTGCTA CAGCACCTCC TGCTATGACA GTAACAATCG CTTCTCCAGC
    CATACCCGCT TCTCCTCTGT TGACAGTGCA AAGATTTCTG AGAGCACGGT CTTTTCCTCA
    CAGGATGAGG AGGAGGAGAA TAGTGCTTTT GAATCGGTGC CAGACTCAGT ACAGAGCCCT
    GAGCTGGACA GGGAGCAAGT GGATGGCTCC TCCCAGTGGC CAGATGAACT AGTAGCTCAT
    GGTGGGAATC CACAACTAGC CGCAGAAAGT CTAGACACCC CAATAGCAGG TCCAAGCAGC
    AGAAGAGAAG GTGATTGTCC TTTACTCCAT AATTCTCAGC CAGTCAGCCA ACTTCCTTCT
    CTGAGGCCTG ACCATCATCA CTACCCAACT ATCGATGAGC CTCTTCCACC AACTGCAACA
    TCCTTGAGAA ACCTTACCCA GTTGGATAAC CAGAGAGCCC TCCCTTTAAA ATTATGTGAT
    GTTCACAGAG AGACTGTATA TTGGGAAGCT CGGATAGACA GCCACGGGAG GGTGTTCTAC
    GTTGACCATG TCAACCGAAC TACCACATGG CAGCGCCCCA CAGCTGCAGC AACACCAGAT
    GGGATCCACA GGTCTGGCTC TATCCAGCAA ATGGAGCAGC TGAACCGGCG ATATCAAAAT
    ATCCAGCGAA CTATTGCAAC AGAGCGGACT GAAGATGATG GTGTAGTAAA CAACAGAGTG
    GAGAGAATTC CCACTGGAGG AGGAAGTGAC TCTGAGGCAG AGCCTTCCCA ACCAAGCTCC
    GAAATTAGGA GGGAAGGTTC CCTTTCTCCT GTGAATTCTC AAAAAATCAC CTTATTGCTG
    CAGTCTCCAG CTGTGAAGTT TATCACCAAT CCTGAGTTCT TCACTGTGCT GCATGCAAAC
    TATAGTGCCT ATCGAGTCTT CACTAACAGT ACCTGCTTGA AGCATATGAT CCTGAAGATC
    CGTAGAGATG CTCGTAATTT TGAGCGCTAT CAGCACAACA GAGACCTTGT AAATTTTATC
    AACATGTTTG CTGACACTCG ACTAGAGCTT CCTCGTGGCT GGGAAATAAA AACTGACCAG
    CAGGGCAAGT CTTTCTTCGT TGACCACAAC AGTCGTGCTA CCACATTCAT AGATCCCAGG
    ATCCCACTGC AGAATGGTCG TCTCCCCAAT CACTTGACTC ACAGGCAACA TCTTCAGCGG
    CTTCGTAGCT ACAGTGCTGG AGAGGCTTCT GAAGTCTCTC GGAACAGAGG AGTTACTCTG
    TTGGCCAGAC CAGGCAATAG TCTTGTAACA GCTATTCGAA ACCAGCATCA TTATGAGACA
    TTACCTCTGG CTTACAATGA CAAGATTGTT GCATTTCTAC GCCAACCTAA CATTTTTGAG
    ATGCTGCAAG AGCGTCAGCC CAGTTTGGCC AGAAATCATG CACTCAGGGA GAAGATCCAT
    TATATTCGGA CAGAGGGTAC ACATGGGCTT GAAAAGTTGT CCTGTGATGC AGATTTAGTA
    ATTCTGCTGA GCCTTTTTGA AGAAGATATC ATGTCCTACA TACCACTCCA GGCCACTTTC
    CATCCTGGTT ACAGTTTCTC TCCTCGCTGC TCACCCTGCT CATCTCCCCA AAATTCTCCA
    GGACTGCAGC GAGCAAGTGC AAGAGCACCC TCTCCATACA GGAGAGATTT TGAAGCCAAA
    CTACGCAATT TCTATCGAAA ACTTGAAGCC AAAGGATATG GACAAGGTCC AGGTAAAATT
    AAGTTAATTA TACGACGTGA CCACTTACTG GAAGGCACCT TCAACCAGGT GATGGCATAT
    TCACGCAAGG AGCTCCAGCG AAACAAACTC TACATCACAT TTGTTGGCGA GGAAGGGCTG
    GACTACAGTG GCCCCTCTCG AGAATTCTTT TTCCTTCTGT CTCAGGAGCT CTTCAATCCA
    TATTACGGGC TGTTTGAGTA TTCAGCCAAT GACACTTACA CTGTACAGAT TAGCCCCATG
    TCTGCCTTTG TTGAAAATCA CCTGGAATGG TTCAGGTTCA GTGGTCGTAT TCTTGGCCTT
    GCTCTCATTC ATCAGTACCT TCTTGATGCT TTCTTCACAA GGCCGTTCTA CAAAGCACTA
    TTAAGGCTGC CATGTGATCT AAGTGACTTA GAGTACCTGG ATGAAGAATT CCATCAGAGC
    TTGCAGTGGA TGAAAGATAA CAACATCACA GATATCCTGG ATCTCACCTT CACAGTGAAT
    GAGGAGGTGT TTGGACAGGT GACAGAGAGA GAGTTGAAAT CTGGAGGGGC AAATACAGCA
    GTGACAGAAA AGAACAAAAA AGAGTACATT GAGAGAATGG TTAAATGGCG AGTGGAACGA
    GGAGTTGTTC AACAAACAGA GGCCCTGGTC CGTGGCTTCT ATGAAGTTGT AGACTCAAGG
    CTTGTCTCTG TTTTTGATGC CAGAGAACTG GAGCTGGTTA TAGCTGGCAC TGCAGAAATA
    GATCTCAATG ACTGGCGTAA CAACACGGAA TATCGTGGAG GTTACCACGA TGGACACATT
    GTAATACGGT GGTTCTGGGC AGCAGTGGAG AGGTTTAACA ATGAACAGAG ACTCCGGTTG
    TTGCAGTTTG TTACTGGAAC ATCAAGTGTG CCATATGAGG GCTTTGCAGC TCTCCGAGGA
    AGCAATGGGC TTCGGCGTTT CTGTATAGAG AAATGGGGGA AAATAACCTC CCTTCCGAGG
    GCACATACAT GTTTCAACCG CTTGGATCTT CCACCTTACC CCTCATATTC TATGCTGTAT
    GAAAAGCTGT TGACAGCTGT TGAAGAAACT AGTACCTTTG GACTTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137106.1
    CDS376..5226
    Translation

    Target ORF information:

    RefSeq Version XM_015281620.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281620.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCTC CATCTAGGAA CTCACAAAAT CGGCGCCGGT GCAAAGAGCC ACTCAGATAC 
    AGCTATAACC CTGACCAGTT TCATAACATG GACATCAGGA ACAATTCCCA TGAGACAGTC
    ACCATTCCTA GGTCTACCAG TGACACGGAC CTTGTCACTT CAGACAGCCG ATCTACCCTG
    ATGGTCAGCA GCTCCTACTA TTCCATTGGG CACTCACAGG ACCTAGTGAT ATACTGGGAT
    ATAAAGGAGG AAGTGGATGC AGGGGACTGG ATTGGCATGT ATCTCATAGA TGAAGTCTTG
    TCTGAAAACT TTTTGGACTA CAAGAACCGT GGAGTCAATG GCTCTCATCG TGGTCAGATT
    GTCTGGAAGA TTGATGCCAG TTCTTACTTT GTGGAGCCTG AAACAAAGAT ATGCTTCAAA
    TACTATCACG GTGTGAGTGG GGCACTACGA GCCACAACTC CAAGCGTCAC GGTGAAAAAC
    AATGCAGCTC CTCAGATTTT TAAAAGCATC ACCAATGAAG ATTCAATCCA GGGACAGGGA
    AATCGGAGGC TGATAAGTTT TTCACTGTCA GATTTCCAAG CTTTTGGTCT GAAGAAAGGA
    ATGTTTTTCA ATCCAGATCC TTATCTGAAG ATTTCCATCC AGCCTGGGAA ACACAGCATC
    TTCCCAGCAC TTCCTCATCA TGGACAGGAG AAAAGGTCTA AGATCATGTG TAATACAGTT
    AACCCAATCT GGCAAGGAGA GCAATTCAGC TTTGTCTCAC TGCCCACGGA TGTCCTGGAA
    ATAGAAGTTA AAGACAAATT TGCAAAGAGC CGACCAATCA TCAAGCGTTT CCTGGGAAAA
    CTCTCTATGC CAGTTCAGCG TCTCTTGGAA AGACATGCCA TAGGGGACAG GGTTGTAAGC
    TACACTCTTG GTCGCAGGCT TCCTACAGAT CATGTCAGTG GCCAGCTGCA GTTCCGATTT
    GAGATAACTT CTTCCATTCA TCAAGATGAT GAAGAGGTTA CCATTAGTGC AGATCCTGAG
    CCAGCTGACC TCCAGGAAAG CCCTGTGAAC AACCCCACAG AGGAAAGCCT CGGTGAGCCT
    CCATGCATCA ACACAGTGAC CTCAGAAAAT ACAGTTCCTG AAGAGCTAAA TGGATACAGT
    GTGAACAATA TTGATAGCCC AGGGGCTGTC AAACCACCCA GGGAAGTCAA CCAGAACAAC
    ATAAATGAAG AGCTAGCAGG AGATGAGGAG GTTGATGCCA TGCCAGTTCC TGAACCCTTG
    GAATCCATCC GAGGAGAAGT GCTGCCTTCT TTGAATGCGA CAGACCTCAC AGAGCAGCTG
    GGTCTGCTGG CTTGCTTGTC TCCTTCTGAG ACTGAAGTTA GGGAAAATAG CAAAGTCAAT
    TCTGGTACTC AGGGAGATGA TGGAGTATTG AACAGTACAG AAACTTTAGA TGTTAGTGAG
    GTGAGTGCAG ATGTATATGC TGACAAAGAC CTAGAGAAGA GTCATGAAGA AGAAGGGGCT
    TCAGCCCGAG GGGAGGAAGA CAGCAAGCTA CAGCTGAGGA CCACAGCAAA GAGGAAAACA
    AGGCCATGCT CCCTGCCTGT GTCTGAAATT GAGACCGTTA TAGCTTCAGC TTGTGGTGAG
    CCAGAGACCC CTCGCACTCA CTATATACGA ATCCACAACC TGCTCCACAG CCTGCCCTCA
    GCACAGATGA GCAGTGATGG GGAAGAAGAG CAAGATGCTG GCAATGGTGG GGAGAATGAT
    GAAGAGTCTA CAGTCAAGGA AGTGTTGCAG AAGGAGACAG TCAGTGAAAG TGAGACAATG
    GCAGCAGAGC CTCCTCCAGA AAATGAGGCC GAAGAGAACT TCAGCCAGTG CACAGTGCCT
    CCTGGGACCT TAGACGAGCA ACTCTCTGAC TTAAATGATG GACTGGTGGA TGGGGAAAAC
    CCTAGGACAG GAAGCGAAAG CGAGTCAAGC TCCAGACCCA ATGGTGACAA CGAATGTGAG
    GCGTGCGACA CCTCTTGCTA CAGCCCCTCC TGCTATAGTA CCTCCTGCTA CAGTACTTCC
    TGTTACAGCA CCTCTTGCTA CAGCACCTCC TGCTATGACA GTAACAATCG CTTCTCCAGC
    CATACCCGCT TCTCCTCTGT TGACAGTGCA AAGATTTCTG AGAGCACGGT CTTTTCCTCA
    CAGGATGAGG AGGAGGAGAA TAGTGCTTTT GAATCGGTGC CAGACTCAGT ACAGAGCCCT
    GAGCTGGACA GGGAGCAAGT GGATGGCTCC TCCCAGTGGC CAGATGAACT AGTAGCTCAT
    GGTGGGAATC CACAACTAGC CGCAGAAAGT CTAGACACCC CAATAGCAGG TCCAAGCAGC
    AGAAGAGAAG GTGATTGTCC TTTACTCCAT AATTCTCAGC CAGTCAGCCA ACTTCCTTCT
    CTGAGGCCTG ACCATCATCA CTACCCAACT ATCGATGAGC CTCTTCCACC AACTGCAACA
    TCCTTGAGAA ACCTTACCCA GTTGGATAAC CAGAGAGCCC TCCCTTTAAA ATTATGTGAT
    GTTCACAGAG AGACTGTATA TTGGGAAGCT CGGATAGACA GCCACGGGAG GGTGTTCTAC
    GTTGACCATG TCAACCGAAC TACCACATGG CAGCGCCCCA CAGCTGCAGC AACACCAGAT
    GGGATCCACA GGTCTGGCTC TATCCAGCAA ATGGAGCAGC TGAACCGGCG ATATCAAAAT
    ATCCAGCGAA CTATTGCAAC AGAGCGGACT GAAGATGATG GTGTAGTAAA CAACAGAGTG
    GAGAGAATTC CCACTGGAGG AGGAAGTGAC TCTGAGGCAG AGCCTTCCCA ACCAAGCTCC
    GAAATTAGGA GGGAAGGTTC CCTTTCTCCT GTGAATTCTC AAAAAATCAC CTTATTGCTG
    CAGTCTCCAG CTGTGAAGTT TATCACCAAT CCTGAGTTCT TCACTGTGCT GCATGCAAAC
    TATAGTGCCT ATCGAGTCTT CACTAACAGT ACCTGCTTGA AGCATATGAT CCTGAAGATC
    CGTAGAGATG CTCGTAATTT TGAGCGCTAT CAGCACAACA GAGACCTTGT AAATTTTATC
    AACATGTTTG CTGACACTCG ACTAGAGCTT CCTCGTGGCT GGGAAATAAA AACTGACCAG
    CAGGGCAAGT CTTTCTTCGT TGACCACAAC AGTCGTGCTA CCACATTCAT AGATCCCAGG
    ATCCCACTGC AGAATGGTCG TCTCCCCAAT CACTTGACTC ACAGGCAACA TCTTCAGCGG
    CTTCGTAGCT ACAGTGCTGG AGAGGCTTCT GAAGTCTCTC GGAACAGAGG AGTTACTCTG
    TTGGCCAGAC CAGGCAATAG TCTTGTAACA GCTATTCGAA ACCAGCATCA TTATGAGACA
    TTACCTCTGG CTTACAATGA CAAGATTGTT GCATTTCTAC GCCAACCTAA CATTTTTGAG
    ATGCTGCAAG AGCGTCAGCC CAGTTTGGCC AGAAATCATG CACTCAGGGA GAAGATCCAT
    TATATTCGGA CAGAGGGTAC ACATGGGCTT GAAAAGTTGT CCTGTGATGC AGATTTAGTA
    ATTCTGCTGA GCCTTTTTGA AGAAGATATC ATGTCCTACA TACCACTCCA GGCCACTTTC
    CATCCTGGTT ACAGTTTCTC TCCTCGCTGC TCACCCTGCT CATCTCCCCA AAATTCTCCA
    GGACTGCAGC GAGCAAGTGC AAGAGCACCC TCTCCATACA GGAGAGATTT TGAAGCCAAA
    CTACGCAATT TCTATCGAAA ACTTGAAGCC AAAGGATATG GACAAGGTCC AGGTAAAATT
    AAGTTAATTA TACGACGTGA CCACTTACTG GAAGGCACCT TCAACCAGGT GATGGCATAT
    TCACGCAAGG AGCTCCAGCG AAACAAACTC TACATCACAT TTGTTGGCGA GGAAGGGCTG
    GACTACAGTG GCCCCTCTCG AGAATTCTTT TTCCTTCTGT CTCAGGAGCT CTTCAATCCA
    TATTACGGGC TGTTTGAGTA TTCAGCCAAT GACACTTACA CTGTACAGAT TAGCCCCATG
    TCTGCCTTTG TTGAAAATCA CCTGGAATGG TTCAGGTTCA GTGGTCGTAT TCTTGGCCTT
    GCTCTCATTC ATCAGTACCT TCTTGATGCT TTCTTCACAA GGCCGTTCTA CAAAGCACTA
    TTAAGGCTGC CATGTGATCT AAGTGACTTA GAGTACCTGG ATGAAGAATT CCATCAGAGC
    TTGCAGTGGA TGAAAGATAA CAACATCACA GATATCCTGG ATCTCACCTT CACAGTGAAT
    GAGGAGGTGT TTGGACAGGT GACAGAGAGA GAGTTGAAAT CTGGAGGGGC AAATACAGCA
    GTGACAGAAA AGAACAAAAA AGAGTACATT GAGAGAATGG TTAAATGGCG AGTGGAACGA
    GGAGTTGTTC AACAAACAGA GGCCCTGGTC CGTGGCTTCT ATGAAGTTGT AGACTCAAGG
    CTTGTCTCTG TTTTTGATGC CAGAGAACTG GAGCTGGTTA TAGCTGGCAC TGCAGAAATA
    GATCTCAATG ACTGGCGTAA CAACACGGAA TATCGTGGAG GTTACCACGA TGGACACATT
    GTAATACGGT GGTTCTGGGC AGCAGTGGAG AGGTTTAACA ATGAACAGAG ACTCCGGTTG
    TTGCAGTTTG TTACTGGAAC ATCAAGTGTG CCATATGAGG GCTTTGCAGC TCTCCGAGGA
    AGCAATGGGC TTCGGCGTTT CTGTATAGAG AAATGGGGGA AAATAACCTC CCTTCCGAGG
    GCACATACAT GTTTCAACCG CTTGGATCTT CCACCTTACC CCTCATATTC TATGCTGTAT
    GAAAAGCTGT TGACAGCTGT TGAAGAAACT AGTACCTTTG GACTTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa31693
    Clone ID Related Accession (Same CDS sequence) XM_015281619.1 , XM_015281619.2 , XM_015281620.1 , XM_015281620.2
    Accession Version XM_015281620.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4851bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281620.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCTC CATCTAGGAA CTCACAAAAT CGGCGCCGGT GCAAAGAGCC ACTCAGATAC 
    AGCTATAACC CTGACCAGTT TCATAACATG GACATCAGGA ACAATTCCCA TGAGACAGTC
    ACCATTCCTA GGTCTACCAG TGACACGGAC CTTGTCACTT CAGACAGCCG ATCTACCCTG
    ATGGTCAGCA GCTCCTACTA TTCCATTGGG CACTCACAGG ACCTAGTGAT ATACTGGGAT
    ATAAAGGAGG AAGTGGATGC AGGGGACTGG ATTGGCATGT ATCTCATAGA TGAAGTCTTG
    TCTGAAAACT TTTTGGACTA CAAGAACCGT GGAGTCAATG GCTCTCATCG TGGTCAGATT
    GTCTGGAAGA TTGATGCCAG TTCTTACTTT GTGGAGCCTG AAACAAAGAT ATGCTTCAAA
    TACTATCACG GTGTGAGTGG GGCACTACGA GCCACAACTC CAAGCGTCAC GGTGAAAAAC
    AATGCAGCTC CTCAGATTTT TAAAAGCATC ACCAATGAAG ATTCAATCCA GGGACAGGGA
    AATCGGAGGC TGATAAGTTT TTCACTGTCA GATTTCCAAG CTTTTGGTCT GAAGAAAGGA
    ATGTTTTTCA ATCCAGATCC TTATCTGAAG ATTTCCATCC AGCCTGGGAA ACACAGCATC
    TTCCCAGCAC TTCCTCATCA TGGACAGGAG AAAAGGTCTA AGATCATGTG TAATACAGTT
    AACCCAATCT GGCAAGGAGA GCAATTCAGC TTTGTCTCAC TGCCCACGGA TGTCCTGGAA
    ATAGAAGTTA AAGACAAATT TGCAAAGAGC CGACCAATCA TCAAGCGTTT CCTGGGAAAA
    CTCTCTATGC CAGTTCAGCG TCTCTTGGAA AGACATGCCA TAGGGGACAG GGTTGTAAGC
    TACACTCTTG GTCGCAGGCT TCCTACAGAT CATGTCAGTG GCCAGCTGCA GTTCCGATTT
    GAGATAACTT CTTCCATTCA TCAAGATGAT GAAGAGGTTA CCATTAGTGC AGATCCTGAG
    CCAGCTGACC TCCAGGAAAG CCCTGTGAAC AACCCCACAG AGGAAAGCCT CGGTGAGCCT
    CCATGCATCA ACACAGTGAC CTCAGAAAAT ACAGTTCCTG AAGAGCTAAA TGGATACAGT
    GTGAACAATA TTGATAGCCC AGGGGCTGTC AAACCACCCA GGGAAGTCAA CCAGAACAAC
    ATAAATGAAG AGCTAGCAGG AGATGAGGAG GTTGATGCCA TGCCAGTTCC TGAACCCTTG
    GAATCCATCC GAGGAGAAGT GCTGCCTTCT TTGAATGCGA CAGACCTCAC AGAGCAGCTG
    GGTCTGCTGG CTTGCTTGTC TCCTTCTGAG ACTGAAGTTA GGGAAAATAG CAAAGTCAAT
    TCTGGTACTC AGGGAGATGA TGGAGTATTG AACAGTACAG AAACTTTAGA TGTTAGTGAG
    GTGAGTGCAG ATGTATATGC TGACAAAGAC CTAGAGAAGA GTCATGAAGA AGAAGGGGCT
    TCAGCCCGAG GGGAGGAAGA CAGCAAGCTA CAGCTGAGGA CCACAGCAAA GAGGAAAACA
    AGGCCATGCT CCCTGCCTGT GTCTGAAATT GAGACCGTTA TAGCTTCAGC TTGTGGTGAG
    CCAGAGACCC CTCGCACTCA CTATATACGA ATCCACAACC TGCTCCACAG CCTGCCCTCA
    GCACAGATGA GCAGTGATGG GGAAGAAGAG CAAGATGCTG GCAATGGTGG GGAGAATGAT
    GAAGAGTCTA CAGTCAAGGA AGTGTTGCAG AAGGAGACAG TCAGTGAAAG TGAGACAATG
    GCAGCAGAGC CTCCTCCAGA AAATGAGGCC GAAGAGAACT TCAGCCAGTG CACAGTGCCT
    CCTGGGACCT TAGACGAGCA ACTCTCTGAC TTAAATGATG GACTGGTGGA TGGGGAAAAC
    CCTAGGACAG GAAGCGAAAG CGAGTCAAGC TCCAGACCCA ATGGTGACAA CGAATGTGAG
    GCGTGCGACA CCTCTTGCTA CAGCCCCTCC TGCTATAGTA CCTCCTGCTA CAGTACTTCC
    TGTTACAGCA CCTCTTGCTA CAGCACCTCC TGCTATGACA GTAACAATCG CTTCTCCAGC
    CATACCCGCT TCTCCTCTGT TGACAGTGCA AAGATTTCTG AGAGCACGGT CTTTTCCTCA
    CAGGATGAGG AGGAGGAGAA TAGTGCTTTT GAATCGGTGC CAGACTCAGT ACAGAGCCCT
    GAGCTGGACA GGGAGCAAGT GGATGGCTCC TCCCAGTGGC CAGATGAACT AGTAGCTCAT
    GGTGGGAATC CACAACTAGC CGCAGAAAGT CTAGACACCC CAATAGCAGG TCCAAGCAGC
    AGAAGAGAAG GTGATTGTCC TTTACTCCAT AATTCTCAGC CAGTCAGCCA ACTTCCTTCT
    CTGAGGCCTG ACCATCATCA CTACCCAACT ATCGATGAGC CTCTTCCACC AACTGCAACA
    TCCTTGAGAA ACCTTACCCA GTTGGATAAC CAGAGAGCCC TCCCTTTAAA ATTATGTGAT
    GTTCACAGAG AGACTGTATA TTGGGAAGCT CGGATAGACA GCCACGGGAG GGTGTTCTAC
    GTTGACCATG TCAACCGAAC TACCACATGG CAGCGCCCCA CAGCTGCAGC AACACCAGAT
    GGGATCCACA GGTCTGGCTC TATCCAGCAA ATGGAGCAGC TGAACCGGCG ATATCAAAAT
    ATCCAGCGAA CTATTGCAAC AGAGCGGACT GAAGATGATG GTGTAGTAAA CAACAGAGTG
    GAGAGAATTC CCACTGGAGG AGGAAGTGAC TCTGAGGCAG AGCCTTCCCA ACCAAGCTCC
    GAAATTAGGA GGGAAGGTTC CCTTTCTCCT GTGAATTCTC AAAAAATCAC CTTATTGCTG
    CAGTCTCCAG CTGTGAAGTT TATCACCAAT CCTGAGTTCT TCACTGTGCT GCATGCAAAC
    TATAGTGCCT ATCGAGTCTT CACTAACAGT ACCTGCTTGA AGCATATGAT CCTGAAGATC
    CGTAGAGATG CTCGTAATTT TGAGCGCTAT CAGCACAACA GAGACCTTGT AAATTTTATC
    AACATGTTTG CTGACACTCG ACTAGAGCTT CCTCGTGGCT GGGAAATAAA AACTGACCAG
    CAGGGCAAGT CTTTCTTCGT TGACCACAAC AGTCGTGCTA CCACATTCAT AGATCCCAGG
    ATCCCACTGC AGAATGGTCG TCTCCCCAAT CACTTGACTC ACAGGCAACA TCTTCAGCGG
    CTTCGTAGCT ACAGTGCTGG AGAGGCTTCT GAAGTCTCTC GGAACAGAGG AGTTACTCTG
    TTGGCCAGAC CAGGCAATAG TCTTGTAACA GCTATTCGAA ACCAGCATCA TTATGAGACA
    TTACCTCTGG CTTACAATGA CAAGATTGTT GCATTTCTAC GCCAACCTAA CATTTTTGAG
    ATGCTGCAAG AGCGTCAGCC CAGTTTGGCC AGAAATCATG CACTCAGGGA GAAGATCCAT
    TATATTCGGA CAGAGGGTAC ACATGGGCTT GAAAAGTTGT CCTGTGATGC AGATTTAGTA
    ATTCTGCTGA GCCTTTTTGA AGAAGATATC ATGTCCTACA TACCACTCCA GGCCACTTTC
    CATCCTGGTT ACAGTTTCTC TCCTCGCTGC TCACCCTGCT CATCTCCCCA AAATTCTCCA
    GGACTGCAGC GAGCAAGTGC AAGAGCACCC TCTCCATACA GGAGAGATTT TGAAGCCAAA
    CTACGCAATT TCTATCGAAA ACTTGAAGCC AAAGGATATG GACAAGGTCC AGGTAAAATT
    AAGTTAATTA TACGACGTGA CCACTTACTG GAAGGCACCT TCAACCAGGT GATGGCATAT
    TCACGCAAGG AGCTCCAGCG AAACAAACTC TACATCACAT TTGTTGGCGA GGAAGGGCTG
    GACTACAGTG GCCCCTCTCG AGAATTCTTT TTCCTTCTGT CTCAGGAGCT CTTCAATCCA
    TATTACGGGC TGTTTGAGTA TTCAGCCAAT GACACTTACA CTGTACAGAT TAGCCCCATG
    TCTGCCTTTG TTGAAAATCA CCTGGAATGG TTCAGGTTCA GTGGTCGTAT TCTTGGCCTT
    GCTCTCATTC ATCAGTACCT TCTTGATGCT TTCTTCACAA GGCCGTTCTA CAAAGCACTA
    TTAAGGCTGC CATGTGATCT AAGTGACTTA GAGTACCTGG ATGAAGAATT CCATCAGAGC
    TTGCAGTGGA TGAAAGATAA CAACATCACA GATATCCTGG ATCTCACCTT CACAGTGAAT
    GAGGAGGTGT TTGGACAGGT GACAGAGAGA GAGTTGAAAT CTGGAGGGGC AAATACAGCA
    GTGACAGAAA AGAACAAAAA AGAGTACATT GAGAGAATGG TTAAATGGCG AGTGGAACGA
    GGAGTTGTTC AACAAACAGA GGCCCTGGTC CGTGGCTTCT ATGAAGTTGT AGACTCAAGG
    CTTGTCTCTG TTTTTGATGC CAGAGAACTG GAGCTGGTTA TAGCTGGCAC TGCAGAAATA
    GATCTCAATG ACTGGCGTAA CAACACGGAA TATCGTGGAG GTTACCACGA TGGACACATT
    GTAATACGGT GGTTCTGGGC AGCAGTGGAG AGGTTTAACA ATGAACAGAG ACTCCGGTTG
    TTGCAGTTTG TTACTGGAAC ATCAAGTGTG CCATATGAGG GCTTTGCAGC TCTCCGAGGA
    AGCAATGGGC TTCGGCGTTT CTGTATAGAG AAATGGGGGA AAATAACCTC CCTTCCGAGG
    GCACATACAT GTTTCAACCG CTTGGATCTT CCACCTTACC CCTCATATTC TATGCTGTAT
    GAAAAGCTGT TGACAGCTGT TGAAGAAACT AGTACCTTTG GACTTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137106.1
    CDS195..5045
    Translation

    Target ORF information:

    RefSeq Version XM_015281620.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281620.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCGTCTC CATCTAGGAA CTCACAAAAT CGGCGCCGGT GCAAAGAGCC ACTCAGATAC 
    AGCTATAACC CTGACCAGTT TCATAACATG GACATCAGGA ACAATTCCCA TGAGACAGTC
    ACCATTCCTA GGTCTACCAG TGACACGGAC CTTGTCACTT CAGACAGCCG ATCTACCCTG
    ATGGTCAGCA GCTCCTACTA TTCCATTGGG CACTCACAGG ACCTAGTGAT ATACTGGGAT
    ATAAAGGAGG AAGTGGATGC AGGGGACTGG ATTGGCATGT ATCTCATAGA TGAAGTCTTG
    TCTGAAAACT TTTTGGACTA CAAGAACCGT GGAGTCAATG GCTCTCATCG TGGTCAGATT
    GTCTGGAAGA TTGATGCCAG TTCTTACTTT GTGGAGCCTG AAACAAAGAT ATGCTTCAAA
    TACTATCACG GTGTGAGTGG GGCACTACGA GCCACAACTC CAAGCGTCAC GGTGAAAAAC
    AATGCAGCTC CTCAGATTTT TAAAAGCATC ACCAATGAAG ATTCAATCCA GGGACAGGGA
    AATCGGAGGC TGATAAGTTT TTCACTGTCA GATTTCCAAG CTTTTGGTCT GAAGAAAGGA
    ATGTTTTTCA ATCCAGATCC TTATCTGAAG ATTTCCATCC AGCCTGGGAA ACACAGCATC
    TTCCCAGCAC TTCCTCATCA TGGACAGGAG AAAAGGTCTA AGATCATGTG TAATACAGTT
    AACCCAATCT GGCAAGGAGA GCAATTCAGC TTTGTCTCAC TGCCCACGGA TGTCCTGGAA
    ATAGAAGTTA AAGACAAATT TGCAAAGAGC CGACCAATCA TCAAGCGTTT CCTGGGAAAA
    CTCTCTATGC CAGTTCAGCG TCTCTTGGAA AGACATGCCA TAGGGGACAG GGTTGTAAGC
    TACACTCTTG GTCGCAGGCT TCCTACAGAT CATGTCAGTG GCCAGCTGCA GTTCCGATTT
    GAGATAACTT CTTCCATTCA TCAAGATGAT GAAGAGGTTA CCATTAGTGC AGATCCTGAG
    CCAGCTGACC TCCAGGAAAG CCCTGTGAAC AACCCCACAG AGGAAAGCCT CGGTGAGCCT
    CCATGCATCA ACACAGTGAC CTCAGAAAAT ACAGTTCCTG AAGAGCTAAA TGGATACAGT
    GTGAACAATA TTGATAGCCC AGGGGCTGTC AAACCACCCA GGGAAGTCAA CCAGAACAAC
    ATAAATGAAG AGCTAGCAGG AGATGAGGAG GTTGATGCCA TGCCAGTTCC TGAACCCTTG
    GAATCCATCC GAGGAGAAGT GCTGCCTTCT TTGAATGCGA CAGACCTCAC AGAGCAGCTG
    GGTCTGCTGG CTTGCTTGTC TCCTTCTGAG ACTGAAGTTA GGGAAAATAG CAAAGTCAAT
    TCTGGTACTC AGGGAGATGA TGGAGTATTG AACAGTACAG AAACTTTAGA TGTTAGTGAG
    GTGAGTGCAG ATGTATATGC TGACAAAGAC CTAGAGAAGA GTCATGAAGA AGAAGGGGCT
    TCAGCCCGAG GGGAGGAAGA CAGCAAGCTA CAGCTGAGGA CCACAGCAAA GAGGAAAACA
    AGGCCATGCT CCCTGCCTGT GTCTGAAATT GAGACCGTTA TAGCTTCAGC TTGTGGTGAG
    CCAGAGACCC CTCGCACTCA CTATATACGA ATCCACAACC TGCTCCACAG CCTGCCCTCA
    GCACAGATGA GCAGTGATGG GGAAGAAGAG CAAGATGCTG GCAATGGTGG GGAGAATGAT
    GAAGAGTCTA CAGTCAAGGA AGTGTTGCAG AAGGAGACAG TCAGTGAAAG TGAGACAATG
    GCAGCAGAGC CTCCTCCAGA AAATGAGGCC GAAGAGAACT TCAGCCAGTG CACAGTGCCT
    CCTGGGACCT TAGACGAGCA ACTCTCTGAC TTAAATGATG GACTGGTGGA TGGGGAAAAC
    CCTAGGACAG GAAGCGAAAG CGAGTCAAGC TCCAGACCCA ATGGTGACAA CGAATGTGAG
    GCGTGCGACA CCTCTTGCTA CAGCCCCTCC TGCTATAGTA CCTCCTGCTA CAGTACTTCC
    TGTTACAGCA CCTCTTGCTA CAGCACCTCC TGCTATGACA GTAACAATCG CTTCTCCAGC
    CATACCCGCT TCTCCTCTGT TGACAGTGCA AAGATTTCTG AGAGCACGGT CTTTTCCTCA
    CAGGATGAGG AGGAGGAGAA TAGTGCTTTT GAATCGGTGC CAGACTCAGT ACAGAGCCCT
    GAGCTGGACA GGGAGCAAGT GGATGGCTCC TCCCAGTGGC CAGATGAACT AGTAGCTCAT
    GGTGGGAATC CACAACTAGC CGCAGAAAGT CTAGACACCC CAATAGCAGG TCCAAGCAGC
    AGAAGAGAAG GTGATTGTCC TTTACTCCAT AATTCTCAGC CAGTCAGCCA ACTTCCTTCT
    CTGAGGCCTG ACCATCATCA CTACCCAACT ATCGATGAGC CTCTTCCACC AACTGCAACA
    TCCTTGAGAA ACCTTACCCA GTTGGATAAC CAGAGAGCCC TCCCTTTAAA ATTATGTGAT
    GTTCACAGAG AGACTGTATA TTGGGAAGCT CGGATAGACA GCCACGGGAG GGTGTTCTAC
    GTTGACCATG TCAACCGAAC TACCACATGG CAGCGCCCCA CAGCTGCAGC AACACCAGAT
    GGGATCCACA GGTCTGGCTC TATCCAGCAA ATGGAGCAGC TGAACCGGCG ATATCAAAAT
    ATCCAGCGAA CTATTGCAAC AGAGCGGACT GAAGATGATG GTGTAGTAAA CAACAGAGTG
    GAGAGAATTC CCACTGGAGG AGGAAGTGAC TCTGAGGCAG AGCCTTCCCA ACCAAGCTCC
    GAAATTAGGA GGGAAGGTTC CCTTTCTCCT GTGAATTCTC AAAAAATCAC CTTATTGCTG
    CAGTCTCCAG CTGTGAAGTT TATCACCAAT CCTGAGTTCT TCACTGTGCT GCATGCAAAC
    TATAGTGCCT ATCGAGTCTT CACTAACAGT ACCTGCTTGA AGCATATGAT CCTGAAGATC
    CGTAGAGATG CTCGTAATTT TGAGCGCTAT CAGCACAACA GAGACCTTGT AAATTTTATC
    AACATGTTTG CTGACACTCG ACTAGAGCTT CCTCGTGGCT GGGAAATAAA AACTGACCAG
    CAGGGCAAGT CTTTCTTCGT TGACCACAAC AGTCGTGCTA CCACATTCAT AGATCCCAGG
    ATCCCACTGC AGAATGGTCG TCTCCCCAAT CACTTGACTC ACAGGCAACA TCTTCAGCGG
    CTTCGTAGCT ACAGTGCTGG AGAGGCTTCT GAAGTCTCTC GGAACAGAGG AGTTACTCTG
    TTGGCCAGAC CAGGCAATAG TCTTGTAACA GCTATTCGAA ACCAGCATCA TTATGAGACA
    TTACCTCTGG CTTACAATGA CAAGATTGTT GCATTTCTAC GCCAACCTAA CATTTTTGAG
    ATGCTGCAAG AGCGTCAGCC CAGTTTGGCC AGAAATCATG CACTCAGGGA GAAGATCCAT
    TATATTCGGA CAGAGGGTAC ACATGGGCTT GAAAAGTTGT CCTGTGATGC AGATTTAGTA
    ATTCTGCTGA GCCTTTTTGA AGAAGATATC ATGTCCTACA TACCACTCCA GGCCACTTTC
    CATCCTGGTT ACAGTTTCTC TCCTCGCTGC TCACCCTGCT CATCTCCCCA AAATTCTCCA
    GGACTGCAGC GAGCAAGTGC AAGAGCACCC TCTCCATACA GGAGAGATTT TGAAGCCAAA
    CTACGCAATT TCTATCGAAA ACTTGAAGCC AAAGGATATG GACAAGGTCC AGGTAAAATT
    AAGTTAATTA TACGACGTGA CCACTTACTG GAAGGCACCT TCAACCAGGT GATGGCATAT
    TCACGCAAGG AGCTCCAGCG AAACAAACTC TACATCACAT TTGTTGGCGA GGAAGGGCTG
    GACTACAGTG GCCCCTCTCG AGAATTCTTT TTCCTTCTGT CTCAGGAGCT CTTCAATCCA
    TATTACGGGC TGTTTGAGTA TTCAGCCAAT GACACTTACA CTGTACAGAT TAGCCCCATG
    TCTGCCTTTG TTGAAAATCA CCTGGAATGG TTCAGGTTCA GTGGTCGTAT TCTTGGCCTT
    GCTCTCATTC ATCAGTACCT TCTTGATGCT TTCTTCACAA GGCCGTTCTA CAAAGCACTA
    TTAAGGCTGC CATGTGATCT AAGTGACTTA GAGTACCTGG ATGAAGAATT CCATCAGAGC
    TTGCAGTGGA TGAAAGATAA CAACATCACA GATATCCTGG ATCTCACCTT CACAGTGAAT
    GAGGAGGTGT TTGGACAGGT GACAGAGAGA GAGTTGAAAT CTGGAGGGGC AAATACAGCA
    GTGACAGAAA AGAACAAAAA AGAGTACATT GAGAGAATGG TTAAATGGCG AGTGGAACGA
    GGAGTTGTTC AACAAACAGA GGCCCTGGTC CGTGGCTTCT ATGAAGTTGT AGACTCAAGG
    CTTGTCTCTG TTTTTGATGC CAGAGAACTG GAGCTGGTTA TAGCTGGCAC TGCAGAAATA
    GATCTCAATG ACTGGCGTAA CAACACGGAA TATCGTGGAG GTTACCACGA TGGACACATT
    GTAATACGGT GGTTCTGGGC AGCAGTGGAG AGGTTTAACA ATGAACAGAG ACTCCGGTTG
    TTGCAGTTTG TTACTGGAAC ATCAAGTGTG CCATATGAGG GCTTTGCAGC TCTCCGAGGA
    AGCAATGGGC TTCGGCGTTT CTGTATAGAG AAATGGGGGA AAATAACCTC CCTTCCGAGG
    GCACATACAT GTTTCAACCG CTTGGATCTT CCACCTTACC CCTCATATTC TATGCTGTAT
    GAAAAGCTGT TGACAGCTGT TGAAGAAACT AGTACCTTTG GACTTGAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa31694
    Clone ID Related Accession (Same CDS sequence) XM_418870.5 , XM_418870.6
    Accession Version XM_418870.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_418870.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGATG TACTGGAAGA AATACAGCTA CCGGTTATGA AAGTCCCTCA GAATCTGTAC 
    CAAAACAGGT TCCTGGGCCT CGCAGCCATG GCGTCTCCAT CTAGGAACTC ACAAAATCGG
    CGCCGGTGCA AAGAGCCACT CAGATACAGC TATAACCCTG ACCAGTTTCA TAACATGGAC
    ATCAGGAACA ATTCCCATGA GACAGTCACC ATTCCTAGGT CTACCAGTGA CACGGACCTT
    GTCACTTCAG ACAGCCGATC TACCCTGATG GTCAGCAGCT CCTACTATTC CATTGGGCAC
    TCACAGGACC TAGTGATATA CTGGGATATA AAGGAGGAAG TGGATGCAGG GGACTGGATT
    GGCATGTATC TCATAGATGA AGTCTTGTCT GAAAACTTTT TGGACTACAA GAACCGTGGA
    GTCAATGGCT CTCATCGTGG TCAGATTGTC TGGAAGATTG ATGCCAGTTC TTACTTTGTG
    GAGCCTGAAA CAAAGATATG CTTCAAATAC TATCACGGTG TGAGTGGGGC ACTACGAGCC
    ACAACTCCAA GCGTCACGGT GAAAAACAAT GCAGCTCCTA TTTTTAAAAG CATCACCAAT
    GAAGATTCAA TCCAGGGACA GGGAAATCGG AGGCTGATAA GTTTTTCACT GTCAGATTTC
    CAAGCTTTTG GTCTGAAGAA AGGAATGTTT TTCAATCCAG ATCCTTATCT GAAGATTTCC
    ATCCAGCCTG GGAAACACAG CATCTTCCCA GCACTTCCTC ATCATGGACA GGAGAAAAGG
    TCTAAGATCA TGTGTAATAC AGTTAACCCA ATCTGGCAAG GAGAGCAATT CAGCTTTGTC
    TCACTGCCCA CGGATGTCCT GGAAATAGAA GTTAAAGACA AATTTGCAAA GAGCCGACCA
    ATCATCAAGC GTTTCCTGGG AAAACTCTCT ATGCCAGTTC AGCGTCTCTT GGAAAGACAT
    GCCATAGGGG ACAGGGTTGT AAGCTACACT CTTGGTCGCA GGCTTCCTAC AGATCATGTC
    AGTGGCCAGC TGCAGTTCCG ATTTGAGATA ACTTCTTCCA TTCATCAAGA TGATGAAGAG
    GTTACCATTA GTGCAGATCC TGAGCCAGCT GACCTCCAGG AAAGCCCTGT GAACAACCCC
    ACAGAGGAAA GCCTCGGTGA GCCTCCATGC ATCAACACAG TGACCTCAGA AAATACAGTT
    CCTGAAGAGC TAAATGGATA CAGTGTGAAC AATATTGATA GCCCAGGGGC TGTCAAACCA
    CCCAGGGAAG TCAACCAGAA CAACATAAAT GAAGAGCTAG CAGGAGATGA GGAGGTTGAT
    GCCATGCCAG TTCCTGAACC CTTGGAATCC ATCCGAGGAG AAGTGCTGCC TTCTTTGAAT
    GCGACAGACC TCACAGAGCA GCTGGGTCTG CTGGCTTGCT TGTCTCCTTC TGAGACTGAA
    GTTAGGGAAA ATAGCAAAGT CAATTCTGGT ACTCAGGGAG ATGATGGAGT ATTGAACAGT
    ACAGAAACTT TAGATGTTAG TGAGGTGAGT GCAGATGTAT ATGCTGACAA AGACCTAGAG
    AAGAGTCATG AAGAAGAAGG GGCTTCAGCC CGAGGGGAGG AAGACAGCAA GCTACAGCTG
    AGGACCACAG CAAAGAGGAA AACAAGGCCA TGCTCCCTGC CTGTGTCTGA AATTGAGACC
    GTTATAGCTT CAGCTTGTGG TGAGCCAGAG ACCCCTCGCA CTCACTATAT ACGAATCCAC
    AACCTGCTCC ACAGCCTGCC CTCAGCACAG ATGAGCAGTG ATGGGGAAGA AGAGCAAGAT
    GCTGGCAATG GTGGGGAGAA TGATGAAGAG TCTACAGTCA AGGAAGTGTT GCAGAAGGAG
    ACAGTCAGTG AAAGTGAGAC AATGGCAGCA GAGCCTCCTC CAGAAAATGA GGCCGAAGAG
    AACTTCAGCC AGTGCACAGT GCCTCCTGGG ACCTTAGACG AGCAACTCTC TGACTTAAAT
    GATGGACTGG TGGATGGGGA AAACCCTAGG ACAGGAAGCG AAAGCGAGTC AAGCTCCAGA
    CCCAATGGTG ACAACGAATG TGAGGCGTGC GACACCTCTT GCTACAGCCC CTCCTGCTAT
    AGTACCTCCT GCTACAGTAC TTCCTGTTAC AGCACCTCTT GCTACAGCAC CTCCTGCTAT
    GACAGTAACA ATCGCTTCTC CAGCCATACC CGCTTCTCCT CTGTTGACAG TGCAAAGATT
    TCTGAGAGCA CGGTCTTTTC CTCACAGGAT GAGGAGGAGG AGAATAGTGC TTTTGAATCG
    GTGCCAGACT CAGTACAGAG CCCTGAGCTG GACAGGGAGC AAGTGGATGG CTCCTCCCAG
    TGGCCAGATG AACTAGTAGC TCATGGTGGG AATCCACAAC TAGCCGCAGA AAGTCTAGAC
    ACCCCAATAG CAGGTCCAAG CAGCAGAAGA GAAGGTGATT GTCCTTTACT CCATAATTCT
    CAGCCAGTCA GCCAACTTCC TTCTCTGAGG CCTGACCATC ATCACTACCC AACTATCGAT
    GAGCCTCTTC CACCAAATTG GGAAGCTCGG ATAGACAGCC ACGGGAGGGT GTTCTACGTT
    GACCATGTCA ACCGAACTAC CACATGGCAG CGCCCCACAG CTGCAGCAAC ACCAGATGGG
    ATCCACAGGT CTGGCTCTAT CCAGCAAATG GAGCAGCTGA ACCGGCGATA TCAAAATATC
    CAGCGAACTA TTGCAACAGA GCGGACTGAA GATGATGGTG TAGTAAACAA CAGAGTGGAG
    AGAATTCCCA CTGGAGGAGG AAGTGACTCT GAGGCAGAGC CTTCCCAACC AAGCTCCGAA
    ATTAGGAGGG AAGGTTCCCT TTCTCCTGTG AATTCTCAAA AAATCACCTT ATTGCTGCAG
    TCTCCAGCTG TGAAGTTTAT CACCAATCCT GAGTTCTTCA CTGTGCTGCA TGCAAACTAT
    AGTGCCTATC GAGTCTTCAC TAACAGTACC TGCTTGAAGC ATATGATCCT GAAGATCCGT
    AGAGATGCTC GTAATTTTGA GCGCTATCAG CACAACAGAG ACCTTGTAAA TTTTATCAAC
    ATGTTTGCTG ACACTCGACT AGAGCTTCCT CGTGGCTGGG AAATAAAAAC TGACCAGCAG
    GGCAAGTCTT TCTTCGTTGA CCACAACAGT CGTGCTACCA CATTCATAGA TCCCAGGATC
    CCACTGCAGA ATGGTCGTCT CCCCAATCAC TTGACTCACA GGCAACATCT TCAGCGGCTT
    CGTAGCTACA GTGCTGGAGA GGCTTCTGAA GTCTCTCGGA ACAGAGGAGT TACTCTGTTG
    GCCAGACCAG GCAATAGTCT TGTAACAGCT ATTCGAAACC AGCATCATTA TGAGACATTA
    CCTCTGGCTT ACAATGACAA GATTGTTGCA TTTCTACGCC AACCTAACAT TTTTGAGATG
    CTGCAAGAGC GTCAGCCCAG TTTGGCCAGA AATCATGCAC TCAGGGAGAA GATCCATTAT
    ATTCGGACAG AGGGTACACA TGGGCTTGAA AAGTTGTCCT GTGATGCAGA TTTAGTAATT
    CTGCTGAGCC TTTTTGAAGA AGATATCATG TCCTACATAC CACTCCAGGC CACTTTCCAT
    CCTGGTTACA GTTTCTCTCC TCGCTGCTCA CCCTGCTCAT CTCCCCAAAA TTCTCCAGGA
    CTGCAGCGAG CAAGTGCAAG AGCACCCTCT CCATACAGGA GAGATTTTGA AGCCAAACTA
    CGCAATTTCT ATCGAAAACT TGAAGCCAAA GGATATGGAC AAGGTCCAGG TAAAATTAAG
    TTAATTATAC GACGTGACCA CTTACTGGAA GGCACCTTCA ACCAGGTGAT GGCATATTCA
    CGCAAGGAGC TCCAGCGAAA CAAACTCTAC ATCACATTTG TTGGCGAGGA AGGGCTGGAC
    TACAGTGGCC CCTCTCGAGA ATTCTTTTTC CTTCTGTCTC AGGAGCTCTT CAATCCATAT
    TACGGGCTGT TTGAGTATTC AGCCAATGAC ACTTACACTG TACAGATTAG CCCCATGTCT
    GCCTTTGTTG AAAATCACCT GGAATGGTTC AGGTTCAGTG GTCGTATTCT TGGCCTTGCT
    CTCATTCATC AGTACCTTCT TGATGCTTTC TTCACAAGGC CGTTCTACAA AGCACTATTA
    AGGCTGCCAT GTGATCTAAG TGACTTAGAG TACCTGGATG AAGAATTCCA TCAGAGCTTG
    CAGTGGATGA AAGATAACAA CATCACAGAT ATCCTGGATC TCACCTTCAC AGTGAATGAG
    GAGGTGTTTG GACAGGTGAC AGAGAGAGAG TTGAAATCTG GAGGGGCAAA TACAGCAGTG
    ACAGAAAAGA ACAAAAAAGA GTACATTGAG AGAATGGTTA AATGGCGAGT GGAACGAGGA
    GTTGTTCAAC AAACAGAGGC CCTGGTCCGT GGCTTCTATG AAGTTGTAGA CTCAAGGCTT
    GTCTCTGTTT TTGATGCCAG AGAACTGGAG CTGGTTATAG CTGGCACTGC AGAAATAGAT
    CTCAATGACT GGCGTAACAA CACGGAATAT CGTGGAGGTT ACCACGATGG ACACATTGTA
    ATACGGTGGT TCTGGGCAGC AGTGGAGAGG TTTAACAATG AACAGAGACT CCGGTTGTTG
    CAGTTTGTTA CTGGAACATC AAGTGTGCCA TATGAGGGCT TTGCAGCTCT CCGAGGAAGC
    AATGGGCTTC GGCGTTTCTG TATAGAGAAA TGGGGGAAAA TAACCTCCCT TCCGAGGGCA
    CATACATGTT TCAACCGCTT GGATCTTCCA CCTTACCCCT CATATTCTAT GCTGTATGAA
    AAGCTGTTGA CAGCTGTTGA AGAAACTAGT ACCTTTGGAC TTGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_418870.5
    CDS45..4892
    Translation

    Target ORF information:

    RefSeq Version XM_418870.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_418870.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGATG TACTGGAAGA AATACAGCTA CCGGTTATGA AAGTCCCTCA GAATCTGTAC 
    CAAAACAGGT TCCTGGGCCT CGCAGCCATG GCGTCTCCAT CTAGGAACTC ACAAAATCGG
    CGCCGGTGCA AAGAGCCACT CAGATACAGC TATAACCCTG ACCAGTTTCA TAACATGGAC
    ATCAGGAACA ATTCCCATGA GACAGTCACC ATTCCTAGGT CTACCAGTGA CACGGACCTT
    GTCACTTCAG ACAGCCGATC TACCCTGATG GTCAGCAGCT CCTACTATTC CATTGGGCAC
    TCACAGGACC TAGTGATATA CTGGGATATA AAGGAGGAAG TGGATGCAGG GGACTGGATT
    GGCATGTATC TCATAGATGA AGTCTTGTCT GAAAACTTTT TGGACTACAA GAACCGTGGA
    GTCAATGGCT CTCATCGTGG TCAGATTGTC TGGAAGATTG ATGCCAGTTC TTACTTTGTG
    GAGCCTGAAA CAAAGATATG CTTCAAATAC TATCACGGTG TGAGTGGGGC ACTACGAGCC
    ACAACTCCAA GCGTCACGGT GAAAAACAAT GCAGCTCCTA TTTTTAAAAG CATCACCAAT
    GAAGATTCAA TCCAGGGACA GGGAAATCGG AGGCTGATAA GTTTTTCACT GTCAGATTTC
    CAAGCTTTTG GTCTGAAGAA AGGAATGTTT TTCAATCCAG ATCCTTATCT GAAGATTTCC
    ATCCAGCCTG GGAAACACAG CATCTTCCCA GCACTTCCTC ATCATGGACA GGAGAAAAGG
    TCTAAGATCA TGTGTAATAC AGTTAACCCA ATCTGGCAAG GAGAGCAATT CAGCTTTGTC
    TCACTGCCCA CGGATGTCCT GGAAATAGAA GTTAAAGACA AATTTGCAAA GAGCCGACCA
    ATCATCAAGC GTTTCCTGGG AAAACTCTCT ATGCCAGTTC AGCGTCTCTT GGAAAGACAT
    GCCATAGGGG ACAGGGTTGT AAGCTACACT CTTGGTCGCA GGCTTCCTAC AGATCATGTC
    AGTGGCCAGC TGCAGTTCCG ATTTGAGATA ACTTCTTCCA TTCATCAAGA TGATGAAGAG
    GTTACCATTA GTGCAGATCC TGAGCCAGCT GACCTCCAGG AAAGCCCTGT GAACAACCCC
    ACAGAGGAAA GCCTCGGTGA GCCTCCATGC ATCAACACAG TGACCTCAGA AAATACAGTT
    CCTGAAGAGC TAAATGGATA CAGTGTGAAC AATATTGATA GCCCAGGGGC TGTCAAACCA
    CCCAGGGAAG TCAACCAGAA CAACATAAAT GAAGAGCTAG CAGGAGATGA GGAGGTTGAT
    GCCATGCCAG TTCCTGAACC CTTGGAATCC ATCCGAGGAG AAGTGCTGCC TTCTTTGAAT
    GCGACAGACC TCACAGAGCA GCTGGGTCTG CTGGCTTGCT TGTCTCCTTC TGAGACTGAA
    GTTAGGGAAA ATAGCAAAGT CAATTCTGGT ACTCAGGGAG ATGATGGAGT ATTGAACAGT
    ACAGAAACTT TAGATGTTAG TGAGGTGAGT GCAGATGTAT ATGCTGACAA AGACCTAGAG
    AAGAGTCATG AAGAAGAAGG GGCTTCAGCC CGAGGGGAGG AAGACAGCAA GCTACAGCTG
    AGGACCACAG CAAAGAGGAA AACAAGGCCA TGCTCCCTGC CTGTGTCTGA AATTGAGACC
    GTTATAGCTT CAGCTTGTGG TGAGCCAGAG ACCCCTCGCA CTCACTATAT ACGAATCCAC
    AACCTGCTCC ACAGCCTGCC CTCAGCACAG ATGAGCAGTG ATGGGGAAGA AGAGCAAGAT
    GCTGGCAATG GTGGGGAGAA TGATGAAGAG TCTACAGTCA AGGAAGTGTT GCAGAAGGAG
    ACAGTCAGTG AAAGTGAGAC AATGGCAGCA GAGCCTCCTC CAGAAAATGA GGCCGAAGAG
    AACTTCAGCC AGTGCACAGT GCCTCCTGGG ACCTTAGACG AGCAACTCTC TGACTTAAAT
    GATGGACTGG TGGATGGGGA AAACCCTAGG ACAGGAAGCG AAAGCGAGTC AAGCTCCAGA
    CCCAATGGTG ACAACGAATG TGAGGCGTGC GACACCTCTT GCTACAGCCC CTCCTGCTAT
    AGTACCTCCT GCTACAGTAC TTCCTGTTAC AGCACCTCTT GCTACAGCAC CTCCTGCTAT
    GACAGTAACA ATCGCTTCTC CAGCCATACC CGCTTCTCCT CTGTTGACAG TGCAAAGATT
    TCTGAGAGCA CGGTCTTTTC CTCACAGGAT GAGGAGGAGG AGAATAGTGC TTTTGAATCG
    GTGCCAGACT CAGTACAGAG CCCTGAGCTG GACAGGGAGC AAGTGGATGG CTCCTCCCAG
    TGGCCAGATG AACTAGTAGC TCATGGTGGG AATCCACAAC TAGCCGCAGA AAGTCTAGAC
    ACCCCAATAG CAGGTCCAAG CAGCAGAAGA GAAGGTGATT GTCCTTTACT CCATAATTCT
    CAGCCAGTCA GCCAACTTCC TTCTCTGAGG CCTGACCATC ATCACTACCC AACTATCGAT
    GAGCCTCTTC CACCAAATTG GGAAGCTCGG ATAGACAGCC ACGGGAGGGT GTTCTACGTT
    GACCATGTCA ACCGAACTAC CACATGGCAG CGCCCCACAG CTGCAGCAAC ACCAGATGGG
    ATCCACAGGT CTGGCTCTAT CCAGCAAATG GAGCAGCTGA ACCGGCGATA TCAAAATATC
    CAGCGAACTA TTGCAACAGA GCGGACTGAA GATGATGGTG TAGTAAACAA CAGAGTGGAG
    AGAATTCCCA CTGGAGGAGG AAGTGACTCT GAGGCAGAGC CTTCCCAACC AAGCTCCGAA
    ATTAGGAGGG AAGGTTCCCT TTCTCCTGTG AATTCTCAAA AAATCACCTT ATTGCTGCAG
    TCTCCAGCTG TGAAGTTTAT CACCAATCCT GAGTTCTTCA CTGTGCTGCA TGCAAACTAT
    AGTGCCTATC GAGTCTTCAC TAACAGTACC TGCTTGAAGC ATATGATCCT GAAGATCCGT
    AGAGATGCTC GTAATTTTGA GCGCTATCAG CACAACAGAG ACCTTGTAAA TTTTATCAAC
    ATGTTTGCTG ACACTCGACT AGAGCTTCCT CGTGGCTGGG AAATAAAAAC TGACCAGCAG
    GGCAAGTCTT TCTTCGTTGA CCACAACAGT CGTGCTACCA CATTCATAGA TCCCAGGATC
    CCACTGCAGA ATGGTCGTCT CCCCAATCAC TTGACTCACA GGCAACATCT TCAGCGGCTT
    CGTAGCTACA GTGCTGGAGA GGCTTCTGAA GTCTCTCGGA ACAGAGGAGT TACTCTGTTG
    GCCAGACCAG GCAATAGTCT TGTAACAGCT ATTCGAAACC AGCATCATTA TGAGACATTA
    CCTCTGGCTT ACAATGACAA GATTGTTGCA TTTCTACGCC AACCTAACAT TTTTGAGATG
    CTGCAAGAGC GTCAGCCCAG TTTGGCCAGA AATCATGCAC TCAGGGAGAA GATCCATTAT
    ATTCGGACAG AGGGTACACA TGGGCTTGAA AAGTTGTCCT GTGATGCAGA TTTAGTAATT
    CTGCTGAGCC TTTTTGAAGA AGATATCATG TCCTACATAC CACTCCAGGC CACTTTCCAT
    CCTGGTTACA GTTTCTCTCC TCGCTGCTCA CCCTGCTCAT CTCCCCAAAA TTCTCCAGGA
    CTGCAGCGAG CAAGTGCAAG AGCACCCTCT CCATACAGGA GAGATTTTGA AGCCAAACTA
    CGCAATTTCT ATCGAAAACT TGAAGCCAAA GGATATGGAC AAGGTCCAGG TAAAATTAAG
    TTAATTATAC GACGTGACCA CTTACTGGAA GGCACCTTCA ACCAGGTGAT GGCATATTCA
    CGCAAGGAGC TCCAGCGAAA CAAACTCTAC ATCACATTTG TTGGCGAGGA AGGGCTGGAC
    TACAGTGGCC CCTCTCGAGA ATTCTTTTTC CTTCTGTCTC AGGAGCTCTT CAATCCATAT
    TACGGGCTGT TTGAGTATTC AGCCAATGAC ACTTACACTG TACAGATTAG CCCCATGTCT
    GCCTTTGTTG AAAATCACCT GGAATGGTTC AGGTTCAGTG GTCGTATTCT TGGCCTTGCT
    CTCATTCATC AGTACCTTCT TGATGCTTTC TTCACAAGGC CGTTCTACAA AGCACTATTA
    AGGCTGCCAT GTGATCTAAG TGACTTAGAG TACCTGGATG AAGAATTCCA TCAGAGCTTG
    CAGTGGATGA AAGATAACAA CATCACAGAT ATCCTGGATC TCACCTTCAC AGTGAATGAG
    GAGGTGTTTG GACAGGTGAC AGAGAGAGAG TTGAAATCTG GAGGGGCAAA TACAGCAGTG
    ACAGAAAAGA ACAAAAAAGA GTACATTGAG AGAATGGTTA AATGGCGAGT GGAACGAGGA
    GTTGTTCAAC AAACAGAGGC CCTGGTCCGT GGCTTCTATG AAGTTGTAGA CTCAAGGCTT
    GTCTCTGTTT TTGATGCCAG AGAACTGGAG CTGGTTATAG CTGGCACTGC AGAAATAGAT
    CTCAATGACT GGCGTAACAA CACGGAATAT CGTGGAGGTT ACCACGATGG ACACATTGTA
    ATACGGTGGT TCTGGGCAGC AGTGGAGAGG TTTAACAATG AACAGAGACT CCGGTTGTTG
    CAGTTTGTTA CTGGAACATC AAGTGTGCCA TATGAGGGCT TTGCAGCTCT CCGAGGAAGC
    AATGGGCTTC GGCGTTTCTG TATAGAGAAA TGGGGGAAAA TAACCTCCCT TCCGAGGGCA
    CATACATGTT TCAACCGCTT GGATCTTCCA CCTTACCCCT CATATTCTAT GCTGTATGAA
    AAGCTGTTGA CAGCTGTTGA AGAAACTAGT ACCTTTGGAC TTGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa31694
    Clone ID Related Accession (Same CDS sequence) XM_418870.5 , XM_418870.6
    Accession Version XM_418870.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_418870.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGATG TACTGGAAGA AATACAGCTA CCGGTTATGA AAGTCCCTCA GAATCTGTAC 
    CAAAACAGGT TCCTGGGCCT CGCAGCCATG GCGTCTCCAT CTAGGAACTC ACAAAATCGG
    CGCCGGTGCA AAGAGCCACT CAGATACAGC TATAACCCTG ACCAGTTTCA TAACATGGAC
    ATCAGGAACA ATTCCCATGA GACAGTCACC ATTCCTAGGT CTACCAGTGA CACGGACCTT
    GTCACTTCAG ACAGCCGATC TACCCTGATG GTCAGCAGCT CCTACTATTC CATTGGGCAC
    TCACAGGACC TAGTGATATA CTGGGATATA AAGGAGGAAG TGGATGCAGG GGACTGGATT
    GGCATGTATC TCATAGATGA AGTCTTGTCT GAAAACTTTT TGGACTACAA GAACCGTGGA
    GTCAATGGCT CTCATCGTGG TCAGATTGTC TGGAAGATTG ATGCCAGTTC TTACTTTGTG
    GAGCCTGAAA CAAAGATATG CTTCAAATAC TATCACGGTG TGAGTGGGGC ACTACGAGCC
    ACAACTCCAA GCGTCACGGT GAAAAACAAT GCAGCTCCTA TTTTTAAAAG CATCACCAAT
    GAAGATTCAA TCCAGGGACA GGGAAATCGG AGGCTGATAA GTTTTTCACT GTCAGATTTC
    CAAGCTTTTG GTCTGAAGAA AGGAATGTTT TTCAATCCAG ATCCTTATCT GAAGATTTCC
    ATCCAGCCTG GGAAACACAG CATCTTCCCA GCACTTCCTC ATCATGGACA GGAGAAAAGG
    TCTAAGATCA TGTGTAATAC AGTTAACCCA ATCTGGCAAG GAGAGCAATT CAGCTTTGTC
    TCACTGCCCA CGGATGTCCT GGAAATAGAA GTTAAAGACA AATTTGCAAA GAGCCGACCA
    ATCATCAAGC GTTTCCTGGG AAAACTCTCT ATGCCAGTTC AGCGTCTCTT GGAAAGACAT
    GCCATAGGGG ACAGGGTTGT AAGCTACACT CTTGGTCGCA GGCTTCCTAC AGATCATGTC
    AGTGGCCAGC TGCAGTTCCG ATTTGAGATA ACTTCTTCCA TTCATCAAGA TGATGAAGAG
    GTTACCATTA GTGCAGATCC TGAGCCAGCT GACCTCCAGG AAAGCCCTGT GAACAACCCC
    ACAGAGGAAA GCCTCGGTGA GCCTCCATGC ATCAACACAG TGACCTCAGA AAATACAGTT
    CCTGAAGAGC TAAATGGATA CAGTGTGAAC AATATTGATA GCCCAGGGGC TGTCAAACCA
    CCCAGGGAAG TCAACCAGAA CAACATAAAT GAAGAGCTAG CAGGAGATGA GGAGGTTGAT
    GCCATGCCAG TTCCTGAACC CTTGGAATCC ATCCGAGGAG AAGTGCTGCC TTCTTTGAAT
    GCGACAGACC TCACAGAGCA GCTGGGTCTG CTGGCTTGCT TGTCTCCTTC TGAGACTGAA
    GTTAGGGAAA ATAGCAAAGT CAATTCTGGT ACTCAGGGAG ATGATGGAGT ATTGAACAGT
    ACAGAAACTT TAGATGTTAG TGAGGTGAGT GCAGATGTAT ATGCTGACAA AGACCTAGAG
    AAGAGTCATG AAGAAGAAGG GGCTTCAGCC CGAGGGGAGG AAGACAGCAA GCTACAGCTG
    AGGACCACAG CAAAGAGGAA AACAAGGCCA TGCTCCCTGC CTGTGTCTGA AATTGAGACC
    GTTATAGCTT CAGCTTGTGG TGAGCCAGAG ACCCCTCGCA CTCACTATAT ACGAATCCAC
    AACCTGCTCC ACAGCCTGCC CTCAGCACAG ATGAGCAGTG ATGGGGAAGA AGAGCAAGAT
    GCTGGCAATG GTGGGGAGAA TGATGAAGAG TCTACAGTCA AGGAAGTGTT GCAGAAGGAG
    ACAGTCAGTG AAAGTGAGAC AATGGCAGCA GAGCCTCCTC CAGAAAATGA GGCCGAAGAG
    AACTTCAGCC AGTGCACAGT GCCTCCTGGG ACCTTAGACG AGCAACTCTC TGACTTAAAT
    GATGGACTGG TGGATGGGGA AAACCCTAGG ACAGGAAGCG AAAGCGAGTC AAGCTCCAGA
    CCCAATGGTG ACAACGAATG TGAGGCGTGC GACACCTCTT GCTACAGCCC CTCCTGCTAT
    AGTACCTCCT GCTACAGTAC TTCCTGTTAC AGCACCTCTT GCTACAGCAC CTCCTGCTAT
    GACAGTAACA ATCGCTTCTC CAGCCATACC CGCTTCTCCT CTGTTGACAG TGCAAAGATT
    TCTGAGAGCA CGGTCTTTTC CTCACAGGAT GAGGAGGAGG AGAATAGTGC TTTTGAATCG
    GTGCCAGACT CAGTACAGAG CCCTGAGCTG GACAGGGAGC AAGTGGATGG CTCCTCCCAG
    TGGCCAGATG AACTAGTAGC TCATGGTGGG AATCCACAAC TAGCCGCAGA AAGTCTAGAC
    ACCCCAATAG CAGGTCCAAG CAGCAGAAGA GAAGGTGATT GTCCTTTACT CCATAATTCT
    CAGCCAGTCA GCCAACTTCC TTCTCTGAGG CCTGACCATC ATCACTACCC AACTATCGAT
    GAGCCTCTTC CACCAAATTG GGAAGCTCGG ATAGACAGCC ACGGGAGGGT GTTCTACGTT
    GACCATGTCA ACCGAACTAC CACATGGCAG CGCCCCACAG CTGCAGCAAC ACCAGATGGG
    ATCCACAGGT CTGGCTCTAT CCAGCAAATG GAGCAGCTGA ACCGGCGATA TCAAAATATC
    CAGCGAACTA TTGCAACAGA GCGGACTGAA GATGATGGTG TAGTAAACAA CAGAGTGGAG
    AGAATTCCCA CTGGAGGAGG AAGTGACTCT GAGGCAGAGC CTTCCCAACC AAGCTCCGAA
    ATTAGGAGGG AAGGTTCCCT TTCTCCTGTG AATTCTCAAA AAATCACCTT ATTGCTGCAG
    TCTCCAGCTG TGAAGTTTAT CACCAATCCT GAGTTCTTCA CTGTGCTGCA TGCAAACTAT
    AGTGCCTATC GAGTCTTCAC TAACAGTACC TGCTTGAAGC ATATGATCCT GAAGATCCGT
    AGAGATGCTC GTAATTTTGA GCGCTATCAG CACAACAGAG ACCTTGTAAA TTTTATCAAC
    ATGTTTGCTG ACACTCGACT AGAGCTTCCT CGTGGCTGGG AAATAAAAAC TGACCAGCAG
    GGCAAGTCTT TCTTCGTTGA CCACAACAGT CGTGCTACCA CATTCATAGA TCCCAGGATC
    CCACTGCAGA ATGGTCGTCT CCCCAATCAC TTGACTCACA GGCAACATCT TCAGCGGCTT
    CGTAGCTACA GTGCTGGAGA GGCTTCTGAA GTCTCTCGGA ACAGAGGAGT TACTCTGTTG
    GCCAGACCAG GCAATAGTCT TGTAACAGCT ATTCGAAACC AGCATCATTA TGAGACATTA
    CCTCTGGCTT ACAATGACAA GATTGTTGCA TTTCTACGCC AACCTAACAT TTTTGAGATG
    CTGCAAGAGC GTCAGCCCAG TTTGGCCAGA AATCATGCAC TCAGGGAGAA GATCCATTAT
    ATTCGGACAG AGGGTACACA TGGGCTTGAA AAGTTGTCCT GTGATGCAGA TTTAGTAATT
    CTGCTGAGCC TTTTTGAAGA AGATATCATG TCCTACATAC CACTCCAGGC CACTTTCCAT
    CCTGGTTACA GTTTCTCTCC TCGCTGCTCA CCCTGCTCAT CTCCCCAAAA TTCTCCAGGA
    CTGCAGCGAG CAAGTGCAAG AGCACCCTCT CCATACAGGA GAGATTTTGA AGCCAAACTA
    CGCAATTTCT ATCGAAAACT TGAAGCCAAA GGATATGGAC AAGGTCCAGG TAAAATTAAG
    TTAATTATAC GACGTGACCA CTTACTGGAA GGCACCTTCA ACCAGGTGAT GGCATATTCA
    CGCAAGGAGC TCCAGCGAAA CAAACTCTAC ATCACATTTG TTGGCGAGGA AGGGCTGGAC
    TACAGTGGCC CCTCTCGAGA ATTCTTTTTC CTTCTGTCTC AGGAGCTCTT CAATCCATAT
    TACGGGCTGT TTGAGTATTC AGCCAATGAC ACTTACACTG TACAGATTAG CCCCATGTCT
    GCCTTTGTTG AAAATCACCT GGAATGGTTC AGGTTCAGTG GTCGTATTCT TGGCCTTGCT
    CTCATTCATC AGTACCTTCT TGATGCTTTC TTCACAAGGC CGTTCTACAA AGCACTATTA
    AGGCTGCCAT GTGATCTAAG TGACTTAGAG TACCTGGATG AAGAATTCCA TCAGAGCTTG
    CAGTGGATGA AAGATAACAA CATCACAGAT ATCCTGGATC TCACCTTCAC AGTGAATGAG
    GAGGTGTTTG GACAGGTGAC AGAGAGAGAG TTGAAATCTG GAGGGGCAAA TACAGCAGTG
    ACAGAAAAGA ACAAAAAAGA GTACATTGAG AGAATGGTTA AATGGCGAGT GGAACGAGGA
    GTTGTTCAAC AAACAGAGGC CCTGGTCCGT GGCTTCTATG AAGTTGTAGA CTCAAGGCTT
    GTCTCTGTTT TTGATGCCAG AGAACTGGAG CTGGTTATAG CTGGCACTGC AGAAATAGAT
    CTCAATGACT GGCGTAACAA CACGGAATAT CGTGGAGGTT ACCACGATGG ACACATTGTA
    ATACGGTGGT TCTGGGCAGC AGTGGAGAGG TTTAACAATG AACAGAGACT CCGGTTGTTG
    CAGTTTGTTA CTGGAACATC AAGTGTGCCA TATGAGGGCT TTGCAGCTCT CCGAGGAAGC
    AATGGGCTTC GGCGTTTCTG TATAGAGAAA TGGGGGAAAA TAACCTCCCT TCCGAGGGCA
    CATACATGTT TCAACCGCTT GGATCTTCCA CCTTACCCCT CATATTCTAT GCTGTATGAA
    AAGCTGTTGA CAGCTGTTGA AGAAACTAGT ACCTTTGGAC TTGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_418870.5
    CDS166..5013
    Translation

    Target ORF information:

    RefSeq Version XM_418870.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_418870.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGGGGATG TACTGGAAGA AATACAGCTA CCGGTTATGA AAGTCCCTCA GAATCTGTAC 
    CAAAACAGGT TCCTGGGCCT CGCAGCCATG GCGTCTCCAT CTAGGAACTC ACAAAATCGG
    CGCCGGTGCA AAGAGCCACT CAGATACAGC TATAACCCTG ACCAGTTTCA TAACATGGAC
    ATCAGGAACA ATTCCCATGA GACAGTCACC ATTCCTAGGT CTACCAGTGA CACGGACCTT
    GTCACTTCAG ACAGCCGATC TACCCTGATG GTCAGCAGCT CCTACTATTC CATTGGGCAC
    TCACAGGACC TAGTGATATA CTGGGATATA AAGGAGGAAG TGGATGCAGG GGACTGGATT
    GGCATGTATC TCATAGATGA AGTCTTGTCT GAAAACTTTT TGGACTACAA GAACCGTGGA
    GTCAATGGCT CTCATCGTGG TCAGATTGTC TGGAAGATTG ATGCCAGTTC TTACTTTGTG
    GAGCCTGAAA CAAAGATATG CTTCAAATAC TATCACGGTG TGAGTGGGGC ACTACGAGCC
    ACAACTCCAA GCGTCACGGT GAAAAACAAT GCAGCTCCTA TTTTTAAAAG CATCACCAAT
    GAAGATTCAA TCCAGGGACA GGGAAATCGG AGGCTGATAA GTTTTTCACT GTCAGATTTC
    CAAGCTTTTG GTCTGAAGAA AGGAATGTTT TTCAATCCAG ATCCTTATCT GAAGATTTCC
    ATCCAGCCTG GGAAACACAG CATCTTCCCA GCACTTCCTC ATCATGGACA GGAGAAAAGG
    TCTAAGATCA TGTGTAATAC AGTTAACCCA ATCTGGCAAG GAGAGCAATT CAGCTTTGTC
    TCACTGCCCA CGGATGTCCT GGAAATAGAA GTTAAAGACA AATTTGCAAA GAGCCGACCA
    ATCATCAAGC GTTTCCTGGG AAAACTCTCT ATGCCAGTTC AGCGTCTCTT GGAAAGACAT
    GCCATAGGGG ACAGGGTTGT AAGCTACACT CTTGGTCGCA GGCTTCCTAC AGATCATGTC
    AGTGGCCAGC TGCAGTTCCG ATTTGAGATA ACTTCTTCCA TTCATCAAGA TGATGAAGAG
    GTTACCATTA GTGCAGATCC TGAGCCAGCT GACCTCCAGG AAAGCCCTGT GAACAACCCC
    ACAGAGGAAA GCCTCGGTGA GCCTCCATGC ATCAACACAG TGACCTCAGA AAATACAGTT
    CCTGAAGAGC TAAATGGATA CAGTGTGAAC AATATTGATA GCCCAGGGGC TGTCAAACCA
    CCCAGGGAAG TCAACCAGAA CAACATAAAT GAAGAGCTAG CAGGAGATGA GGAGGTTGAT
    GCCATGCCAG TTCCTGAACC CTTGGAATCC ATCCGAGGAG AAGTGCTGCC TTCTTTGAAT
    GCGACAGACC TCACAGAGCA GCTGGGTCTG CTGGCTTGCT TGTCTCCTTC TGAGACTGAA
    GTTAGGGAAA ATAGCAAAGT CAATTCTGGT ACTCAGGGAG ATGATGGAGT ATTGAACAGT
    ACAGAAACTT TAGATGTTAG TGAGGTGAGT GCAGATGTAT ATGCTGACAA AGACCTAGAG
    AAGAGTCATG AAGAAGAAGG GGCTTCAGCC CGAGGGGAGG AAGACAGCAA GCTACAGCTG
    AGGACCACAG CAAAGAGGAA AACAAGGCCA TGCTCCCTGC CTGTGTCTGA AATTGAGACC
    GTTATAGCTT CAGCTTGTGG TGAGCCAGAG ACCCCTCGCA CTCACTATAT ACGAATCCAC
    AACCTGCTCC ACAGCCTGCC CTCAGCACAG ATGAGCAGTG ATGGGGAAGA AGAGCAAGAT
    GCTGGCAATG GTGGGGAGAA TGATGAAGAG TCTACAGTCA AGGAAGTGTT GCAGAAGGAG
    ACAGTCAGTG AAAGTGAGAC AATGGCAGCA GAGCCTCCTC CAGAAAATGA GGCCGAAGAG
    AACTTCAGCC AGTGCACAGT GCCTCCTGGG ACCTTAGACG AGCAACTCTC TGACTTAAAT
    GATGGACTGG TGGATGGGGA AAACCCTAGG ACAGGAAGCG AAAGCGAGTC AAGCTCCAGA
    CCCAATGGTG ACAACGAATG TGAGGCGTGC GACACCTCTT GCTACAGCCC CTCCTGCTAT
    AGTACCTCCT GCTACAGTAC TTCCTGTTAC AGCACCTCTT GCTACAGCAC CTCCTGCTAT
    GACAGTAACA ATCGCTTCTC CAGCCATACC CGCTTCTCCT CTGTTGACAG TGCAAAGATT
    TCTGAGAGCA CGGTCTTTTC CTCACAGGAT GAGGAGGAGG AGAATAGTGC TTTTGAATCG
    GTGCCAGACT CAGTACAGAG CCCTGAGCTG GACAGGGAGC AAGTGGATGG CTCCTCCCAG
    TGGCCAGATG AACTAGTAGC TCATGGTGGG AATCCACAAC TAGCCGCAGA AAGTCTAGAC
    ACCCCAATAG CAGGTCCAAG CAGCAGAAGA GAAGGTGATT GTCCTTTACT CCATAATTCT
    CAGCCAGTCA GCCAACTTCC TTCTCTGAGG CCTGACCATC ATCACTACCC AACTATCGAT
    GAGCCTCTTC CACCAAATTG GGAAGCTCGG ATAGACAGCC ACGGGAGGGT GTTCTACGTT
    GACCATGTCA ACCGAACTAC CACATGGCAG CGCCCCACAG CTGCAGCAAC ACCAGATGGG
    ATCCACAGGT CTGGCTCTAT CCAGCAAATG GAGCAGCTGA ACCGGCGATA TCAAAATATC
    CAGCGAACTA TTGCAACAGA GCGGACTGAA GATGATGGTG TAGTAAACAA CAGAGTGGAG
    AGAATTCCCA CTGGAGGAGG AAGTGACTCT GAGGCAGAGC CTTCCCAACC AAGCTCCGAA
    ATTAGGAGGG AAGGTTCCCT TTCTCCTGTG AATTCTCAAA AAATCACCTT ATTGCTGCAG
    TCTCCAGCTG TGAAGTTTAT CACCAATCCT GAGTTCTTCA CTGTGCTGCA TGCAAACTAT
    AGTGCCTATC GAGTCTTCAC TAACAGTACC TGCTTGAAGC ATATGATCCT GAAGATCCGT
    AGAGATGCTC GTAATTTTGA GCGCTATCAG CACAACAGAG ACCTTGTAAA TTTTATCAAC
    ATGTTTGCTG ACACTCGACT AGAGCTTCCT CGTGGCTGGG AAATAAAAAC TGACCAGCAG
    GGCAAGTCTT TCTTCGTTGA CCACAACAGT CGTGCTACCA CATTCATAGA TCCCAGGATC
    CCACTGCAGA ATGGTCGTCT CCCCAATCAC TTGACTCACA GGCAACATCT TCAGCGGCTT
    CGTAGCTACA GTGCTGGAGA GGCTTCTGAA GTCTCTCGGA ACAGAGGAGT TACTCTGTTG
    GCCAGACCAG GCAATAGTCT TGTAACAGCT ATTCGAAACC AGCATCATTA TGAGACATTA
    CCTCTGGCTT ACAATGACAA GATTGTTGCA TTTCTACGCC AACCTAACAT TTTTGAGATG
    CTGCAAGAGC GTCAGCCCAG TTTGGCCAGA AATCATGCAC TCAGGGAGAA GATCCATTAT
    ATTCGGACAG AGGGTACACA TGGGCTTGAA AAGTTGTCCT GTGATGCAGA TTTAGTAATT
    CTGCTGAGCC TTTTTGAAGA AGATATCATG TCCTACATAC CACTCCAGGC CACTTTCCAT
    CCTGGTTACA GTTTCTCTCC TCGCTGCTCA CCCTGCTCAT CTCCCCAAAA TTCTCCAGGA
    CTGCAGCGAG CAAGTGCAAG AGCACCCTCT CCATACAGGA GAGATTTTGA AGCCAAACTA
    CGCAATTTCT ATCGAAAACT TGAAGCCAAA GGATATGGAC AAGGTCCAGG TAAAATTAAG
    TTAATTATAC GACGTGACCA CTTACTGGAA GGCACCTTCA ACCAGGTGAT GGCATATTCA
    CGCAAGGAGC TCCAGCGAAA CAAACTCTAC ATCACATTTG TTGGCGAGGA AGGGCTGGAC
    TACAGTGGCC CCTCTCGAGA ATTCTTTTTC CTTCTGTCTC AGGAGCTCTT CAATCCATAT
    TACGGGCTGT TTGAGTATTC AGCCAATGAC ACTTACACTG TACAGATTAG CCCCATGTCT
    GCCTTTGTTG AAAATCACCT GGAATGGTTC AGGTTCAGTG GTCGTATTCT TGGCCTTGCT
    CTCATTCATC AGTACCTTCT TGATGCTTTC TTCACAAGGC CGTTCTACAA AGCACTATTA
    AGGCTGCCAT GTGATCTAAG TGACTTAGAG TACCTGGATG AAGAATTCCA TCAGAGCTTG
    CAGTGGATGA AAGATAACAA CATCACAGAT ATCCTGGATC TCACCTTCAC AGTGAATGAG
    GAGGTGTTTG GACAGGTGAC AGAGAGAGAG TTGAAATCTG GAGGGGCAAA TACAGCAGTG
    ACAGAAAAGA ACAAAAAAGA GTACATTGAG AGAATGGTTA AATGGCGAGT GGAACGAGGA
    GTTGTTCAAC AAACAGAGGC CCTGGTCCGT GGCTTCTATG AAGTTGTAGA CTCAAGGCTT
    GTCTCTGTTT TTGATGCCAG AGAACTGGAG CTGGTTATAG CTGGCACTGC AGAAATAGAT
    CTCAATGACT GGCGTAACAA CACGGAATAT CGTGGAGGTT ACCACGATGG ACACATTGTA
    ATACGGTGGT TCTGGGCAGC AGTGGAGAGG TTTAACAATG AACAGAGACT CCGGTTGTTG
    CAGTTTGTTA CTGGAACATC AAGTGTGCCA TATGAGGGCT TTGCAGCTCT CCGAGGAAGC
    AATGGGCTTC GGCGTTTCTG TATAGAGAAA TGGGGGAAAA TAACCTCCCT TCCGAGGGCA
    CATACATGTT TCAACCGCTT GGATCTTCCA CCTTACCCCT CATATTCTAT GCTGTATGAA
    AAGCTGTTGA CAGCTGTTGA AGAAACTAGT ACCTTTGGAC TTGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa31695
    Clone ID Related Accession (Same CDS sequence) XM_015281621.2 , XM_015281621.1
    Accession Version XM_015281621.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4413bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281621.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAGTTA CAAGAATCCT GCAGTACATG GGCACGAGAT CCTGTAGGCC AATTTGGATT 
    TTTAAAAGCA TCACCAATGA AGATTCAATC CAGGGACAGG GAAATCGGAG GCTGATAAGT
    TTTTCACTGT CAGATTTCCA AGCTTTTGGT CTGAAGAAAG GAATGTTTTT CAATCCAGAT
    CCTTATCTGA AGATTTCCAT CCAGCCTGGG AAACACAGCA TCTTCCCAGC ACTTCCTCAT
    CATGGACAGG AGAAAAGGTC TAAGATCATG TGTAATACAG TTAACCCAAT CTGGCAAGGA
    GAGCAATTCA GCTTTGTCTC ACTGCCCACG GATGTCCTGG AAATAGAAGT TAAAGACAAA
    TTTGCAAAGA GCCGACCAAT CATCAAGCGT TTCCTGGGAA AACTCTCTAT GCCAGTTCAG
    CGTCTCTTGG AAAGACATGC CATAGGGGAC AGGGTTGTAA GCTACACTCT TGGTCGCAGG
    CTTCCTACAG ATCATGTCAG TGGCCAGCTG CAGTTCCGAT TTGAGATAAC TTCTTCCATT
    CATCAAGATG ATGAAGAGGT TACCATTAGT GCAGATCCTG AGCCAGCTGA CCTCCAGGAA
    AGCCCTGTGA ACAACCCCAC AGAGGAAAGC CTCGGTGAGC CTCCATGCAT CAACACAGTG
    ACCTCAGAAA ATACAGTTCC TGAAGAGCTA AATGGATACA GTGTGAACAA TATTGATAGC
    CCAGGGGCTG TCAAACCACC CAGGGAAGTC AACCAGAACA ACATAAATGA AGAGCTAGCA
    GGAGATGAGG AGGTTGATGC CATGCCAGTT CCTGAACCCT TGGAATCCAT CCGAGGAGAA
    GTGCTGCCTT CTTTGAATGC GACAGACCTC ACAGAGCAGC TGGGTCTGCT GGCTTGCTTG
    TCTCCTTCTG AGACTGAAGT TAGGGAAAAT AGCAAAGTCA ATTCTGGTAC TCAGGGAGAT
    GATGGAGTAT TGAACAGTAC AGAAACTTTA GATGTTAGTG AGGTGAGTGC AGATGTATAT
    GCTGACAAAG ACCTAGAGAA GAGTCATGAA GAAGAAGGGG CTTCAGCCCG AGGGGAGGAA
    GACAGCAAGC TACAGCTGAG GACCACAGCA AAGAGGAAAA CAAGGCCATG CTCCCTGCCT
    GTGTCTGAAA TTGAGACCGT TATAGCTTCA GCTTGTGGTG AGCCAGAGAC CCCTCGCACT
    CACTATATAC GAATCCACAA CCTGCTCCAC AGCCTGCCCT CAGCACAGAT GAGCAGTGAT
    GGGGAAGAAG AGCAAGATGC TGGCAATGGT GGGGAGAATG ATGAAGAGTC TACAGTCAAG
    GAAGTGTTGC AGAAGGAGAC AGTCAGTGAA AGTGAGACAA TGGCAGCAGA GCCTCCTCCA
    GAAAATGAGG CCGAAGAGAA CTTCAGCCAG TGCACAGTGC CTCCTGGGAC CTTAGACGAG
    CAACTCTCTG ACTTAAATGA TGGACTGGTG GATGGGGAAA ACCCTAGGAC AGGAAGCGAA
    AGCGAGTCAA GCTCCAGACC CAATGGTGAC AACGAATGTG AGGCGTGCGA CACCTCTTGC
    TACAGCCCCT CCTGCTATAG TACCTCCTGC TACAGTACTT CCTGTTACAG CACCTCTTGC
    TACAGCACCT CCTGCTATGA CAGTAACAAT CGCTTCTCCA GCCATACCCG CTTCTCCTCT
    GTTGACAGTG CAAAGATTTC TGAGAGCACG GTCTTTTCCT CACAGGATGA GGAGGAGGAG
    AATAGTGCTT TTGAATCGGT GCCAGACTCA GTACAGAGCC CTGAGCTGGA CAGGGAGCAA
    GTGGATGGCT CCTCCCAGTG GCCAGATGAA CTAGTAGCTC ATGGTGGGAA TCCACAACTA
    GCCGCAGAAA GTCTAGACAC CCCAATAGCA GGTCCAAGCA GCAGAAGAGA AGGTGATTGT
    CCTTTACTCC ATAATTCTCA GCCAGTCAGC CAACTTCCTT CTCTGAGGCC TGACCATCAT
    CACTACCCAA CTATCGATGA GCCTCTTCCA CCAACTGCAA CATCCTTGAG AAACCTTACC
    CAGTTGGATA ACCAGAGAGC CCTCCCTTTA AAATTATGTG ATGTTCACAG AGAGACTGTA
    TATTGGGAAG CTCGGATAGA CAGCCACGGG AGGGTGTTCT ACGTTGACCA TGTCAACCGA
    ACTACCACAT GGCAGCGCCC CACAGCTGCA GCAACACCAG ATGGGATCCA CAGGTCTGGC
    TCTATCCAGC AAATGGAGCA GCTGAACCGG CGATATCAAA ATATCCAGCG AACTATTGCA
    ACAGAGCGGA CTGAAGATGA TGGTGTAGTA AACAACAGAG TGGAGAGAAT TCCCACTGGA
    GGAGGAAGTG ACTCTGAGGC AGAGCCTTCC CAACCAAGCT CCGAAATTAG GAGGGAAGGT
    TCCCTTTCTC CTGTGAATTC TCAAAAAATC ACCTTATTGC TGCAGTCTCC AGCTGTGAAG
    TTTATCACCA ATCCTGAGTT CTTCACTGTG CTGCATGCAA ACTATAGTGC CTATCGAGTC
    TTCACTAACA GTACCTGCTT GAAGCATATG ATCCTGAAGA TCCGTAGAGA TGCTCGTAAT
    TTTGAGCGCT ATCAGCACAA CAGAGACCTT GTAAATTTTA TCAACATGTT TGCTGACACT
    CGACTAGAGC TTCCTCGTGG CTGGGAAATA AAAACTGACC AGCAGGGCAA GTCTTTCTTC
    GTTGACCACA ACAGTCGTGC TACCACATTC ATAGATCCCA GGATCCCACT GCAGAATGGT
    CGTCTCCCCA ATCACTTGAC TCACAGGCAA CATCTTCAGC GGCTTCGTAG CTACAGTGCT
    GGAGAGGCTT CTGAAGTCTC TCGGAACAGA GGAGTTACTC TGTTGGCCAG ACCAGGCAAT
    AGTCTTGTAA CAGCTATTCG AAACCAGCAT CATTATGAGA CATTACCTCT GGCTTACAAT
    GACAAGATTG TTGCATTTCT ACGCCAACCT AACATTTTTG AGATGCTGCA AGAGCGTCAG
    CCCAGTTTGG CCAGAAATCA TGCACTCAGG GAGAAGATCC ATTATATTCG GACAGAGGGT
    ACACATGGGC TTGAAAAGTT GTCCTGTGAT GCAGATTTAG TAATTCTGCT GAGCCTTTTT
    GAAGAAGATA TCATGTCCTA CATACCACTC CAGGCCACTT TCCATCCTGG TTACAGTTTC
    TCTCCTCGCT GCTCACCCTG CTCATCTCCC CAAAATTCTC CAGGACTGCA GCGAGCAAGT
    GCAAGAGCAC CCTCTCCATA CAGGAGAGAT TTTGAAGCCA AACTACGCAA TTTCTATCGA
    AAACTTGAAG CCAAAGGATA TGGACAAGGT CCAGGTAAAA TTAAGTTAAT TATACGACGT
    GACCACTTAC TGGAAGGCAC CTTCAACCAG GTGATGGCAT ATTCACGCAA GGAGCTCCAG
    CGAAACAAAC TCTACATCAC ATTTGTTGGC GAGGAAGGGC TGGACTACAG TGGCCCCTCT
    CGAGAATTCT TTTTCCTTCT GTCTCAGGAG CTCTTCAATC CATATTACGG GCTGTTTGAG
    TATTCAGCCA ATGACACTTA CACTGTACAG ATTAGCCCCA TGTCTGCCTT TGTTGAAAAT
    CACCTGGAAT GGTTCAGGTT CAGTGGTCGT ATTCTTGGCC TTGCTCTCAT TCATCAGTAC
    CTTCTTGATG CTTTCTTCAC AAGGCCGTTC TACAAAGCAC TATTAAGGCT GCCATGTGAT
    CTAAGTGACT TAGAGTACCT GGATGAAGAA TTCCATCAGA GCTTGCAGTG GATGAAAGAT
    AACAACATCA CAGATATCCT GGATCTCACC TTCACAGTGA ATGAGGAGGT GTTTGGACAG
    GTGACAGAGA GAGAGTTGAA ATCTGGAGGG GCAAATACAG CAGTGACAGA AAAGAACAAA
    AAAGAGTACA TTGAGAGAAT GGTTAAATGG CGAGTGGAAC GAGGAGTTGT TCAACAAACA
    GAGGCCCTGG TCCGTGGCTT CTATGAAGTT GTAGACTCAA GGCTTGTCTC TGTTTTTGAT
    GCCAGAGAAC TGGAGCTGGT TATAGCTGGC ACTGCAGAAA TAGATCTCAA TGACTGGCGT
    AACAACACGG AATATCGTGG AGGTTACCAC GATGGACACA TTGTAATACG GTGGTTCTGG
    GCAGCAGTGG AGAGGTTTAA CAATGAACAG AGACTCCGGT TGTTGCAGTT TGTTACTGGA
    ACATCAAGTG TGCCATATGA GGGCTTTGCA GCTCTCCGAG GAAGCAATGG GCTTCGGCGT
    TTCTGTATAG AGAAATGGGG GAAAATAACC TCCCTTCCGA GGGCACATAC ATGTTTCAAC
    CGCTTGGATC TTCCACCTTA CCCCTCATAT TCTATGCTGT ATGAAAAGCT GTTGACAGCT
    GTTGAAGAAA CTAGTACCTT TGGACTTGAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137107.1
    CDS16..4428
    Translation

    Target ORF information:

    RefSeq Version XM_015281621.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281621.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAGTTA CAAGAATCCT GCAGTACATG GGCACGAGAT CCTGTAGGCC AATTTGGATT 
    TTTAAAAGCA TCACCAATGA AGATTCAATC CAGGGACAGG GAAATCGGAG GCTGATAAGT
    TTTTCACTGT CAGATTTCCA AGCTTTTGGT CTGAAGAAAG GAATGTTTTT CAATCCAGAT
    CCTTATCTGA AGATTTCCAT CCAGCCTGGG AAACACAGCA TCTTCCCAGC ACTTCCTCAT
    CATGGACAGG AGAAAAGGTC TAAGATCATG TGTAATACAG TTAACCCAAT CTGGCAAGGA
    GAGCAATTCA GCTTTGTCTC ACTGCCCACG GATGTCCTGG AAATAGAAGT TAAAGACAAA
    TTTGCAAAGA GCCGACCAAT CATCAAGCGT TTCCTGGGAA AACTCTCTAT GCCAGTTCAG
    CGTCTCTTGG AAAGACATGC CATAGGGGAC AGGGTTGTAA GCTACACTCT TGGTCGCAGG
    CTTCCTACAG ATCATGTCAG TGGCCAGCTG CAGTTCCGAT TTGAGATAAC TTCTTCCATT
    CATCAAGATG ATGAAGAGGT TACCATTAGT GCAGATCCTG AGCCAGCTGA CCTCCAGGAA
    AGCCCTGTGA ACAACCCCAC AGAGGAAAGC CTCGGTGAGC CTCCATGCAT CAACACAGTG
    ACCTCAGAAA ATACAGTTCC TGAAGAGCTA AATGGATACA GTGTGAACAA TATTGATAGC
    CCAGGGGCTG TCAAACCACC CAGGGAAGTC AACCAGAACA ACATAAATGA AGAGCTAGCA
    GGAGATGAGG AGGTTGATGC CATGCCAGTT CCTGAACCCT TGGAATCCAT CCGAGGAGAA
    GTGCTGCCTT CTTTGAATGC GACAGACCTC ACAGAGCAGC TGGGTCTGCT GGCTTGCTTG
    TCTCCTTCTG AGACTGAAGT TAGGGAAAAT AGCAAAGTCA ATTCTGGTAC TCAGGGAGAT
    GATGGAGTAT TGAACAGTAC AGAAACTTTA GATGTTAGTG AGGTGAGTGC AGATGTATAT
    GCTGACAAAG ACCTAGAGAA GAGTCATGAA GAAGAAGGGG CTTCAGCCCG AGGGGAGGAA
    GACAGCAAGC TACAGCTGAG GACCACAGCA AAGAGGAAAA CAAGGCCATG CTCCCTGCCT
    GTGTCTGAAA TTGAGACCGT TATAGCTTCA GCTTGTGGTG AGCCAGAGAC CCCTCGCACT
    CACTATATAC GAATCCACAA CCTGCTCCAC AGCCTGCCCT CAGCACAGAT GAGCAGTGAT
    GGGGAAGAAG AGCAAGATGC TGGCAATGGT GGGGAGAATG ATGAAGAGTC TACAGTCAAG
    GAAGTGTTGC AGAAGGAGAC AGTCAGTGAA AGTGAGACAA TGGCAGCAGA GCCTCCTCCA
    GAAAATGAGG CCGAAGAGAA CTTCAGCCAG TGCACAGTGC CTCCTGGGAC CTTAGACGAG
    CAACTCTCTG ACTTAAATGA TGGACTGGTG GATGGGGAAA ACCCTAGGAC AGGAAGCGAA
    AGCGAGTCAA GCTCCAGACC CAATGGTGAC AACGAATGTG AGGCGTGCGA CACCTCTTGC
    TACAGCCCCT CCTGCTATAG TACCTCCTGC TACAGTACTT CCTGTTACAG CACCTCTTGC
    TACAGCACCT CCTGCTATGA CAGTAACAAT CGCTTCTCCA GCCATACCCG CTTCTCCTCT
    GTTGACAGTG CAAAGATTTC TGAGAGCACG GTCTTTTCCT CACAGGATGA GGAGGAGGAG
    AATAGTGCTT TTGAATCGGT GCCAGACTCA GTACAGAGCC CTGAGCTGGA CAGGGAGCAA
    GTGGATGGCT CCTCCCAGTG GCCAGATGAA CTAGTAGCTC ATGGTGGGAA TCCACAACTA
    GCCGCAGAAA GTCTAGACAC CCCAATAGCA GGTCCAAGCA GCAGAAGAGA AGGTGATTGT
    CCTTTACTCC ATAATTCTCA GCCAGTCAGC CAACTTCCTT CTCTGAGGCC TGACCATCAT
    CACTACCCAA CTATCGATGA GCCTCTTCCA CCAACTGCAA CATCCTTGAG AAACCTTACC
    CAGTTGGATA ACCAGAGAGC CCTCCCTTTA AAATTATGTG ATGTTCACAG AGAGACTGTA
    TATTGGGAAG CTCGGATAGA CAGCCACGGG AGGGTGTTCT ACGTTGACCA TGTCAACCGA
    ACTACCACAT GGCAGCGCCC CACAGCTGCA GCAACACCAG ATGGGATCCA CAGGTCTGGC
    TCTATCCAGC AAATGGAGCA GCTGAACCGG CGATATCAAA ATATCCAGCG AACTATTGCA
    ACAGAGCGGA CTGAAGATGA TGGTGTAGTA AACAACAGAG TGGAGAGAAT TCCCACTGGA
    GGAGGAAGTG ACTCTGAGGC AGAGCCTTCC CAACCAAGCT CCGAAATTAG GAGGGAAGGT
    TCCCTTTCTC CTGTGAATTC TCAAAAAATC ACCTTATTGC TGCAGTCTCC AGCTGTGAAG
    TTTATCACCA ATCCTGAGTT CTTCACTGTG CTGCATGCAA ACTATAGTGC CTATCGAGTC
    TTCACTAACA GTACCTGCTT GAAGCATATG ATCCTGAAGA TCCGTAGAGA TGCTCGTAAT
    TTTGAGCGCT ATCAGCACAA CAGAGACCTT GTAAATTTTA TCAACATGTT TGCTGACACT
    CGACTAGAGC TTCCTCGTGG CTGGGAAATA AAAACTGACC AGCAGGGCAA GTCTTTCTTC
    GTTGACCACA ACAGTCGTGC TACCACATTC ATAGATCCCA GGATCCCACT GCAGAATGGT
    CGTCTCCCCA ATCACTTGAC TCACAGGCAA CATCTTCAGC GGCTTCGTAG CTACAGTGCT
    GGAGAGGCTT CTGAAGTCTC TCGGAACAGA GGAGTTACTC TGTTGGCCAG ACCAGGCAAT
    AGTCTTGTAA CAGCTATTCG AAACCAGCAT CATTATGAGA CATTACCTCT GGCTTACAAT
    GACAAGATTG TTGCATTTCT ACGCCAACCT AACATTTTTG AGATGCTGCA AGAGCGTCAG
    CCCAGTTTGG CCAGAAATCA TGCACTCAGG GAGAAGATCC ATTATATTCG GACAGAGGGT
    ACACATGGGC TTGAAAAGTT GTCCTGTGAT GCAGATTTAG TAATTCTGCT GAGCCTTTTT
    GAAGAAGATA TCATGTCCTA CATACCACTC CAGGCCACTT TCCATCCTGG TTACAGTTTC
    TCTCCTCGCT GCTCACCCTG CTCATCTCCC CAAAATTCTC CAGGACTGCA GCGAGCAAGT
    GCAAGAGCAC CCTCTCCATA CAGGAGAGAT TTTGAAGCCA AACTACGCAA TTTCTATCGA
    AAACTTGAAG CCAAAGGATA TGGACAAGGT CCAGGTAAAA TTAAGTTAAT TATACGACGT
    GACCACTTAC TGGAAGGCAC CTTCAACCAG GTGATGGCAT ATTCACGCAA GGAGCTCCAG
    CGAAACAAAC TCTACATCAC ATTTGTTGGC GAGGAAGGGC TGGACTACAG TGGCCCCTCT
    CGAGAATTCT TTTTCCTTCT GTCTCAGGAG CTCTTCAATC CATATTACGG GCTGTTTGAG
    TATTCAGCCA ATGACACTTA CACTGTACAG ATTAGCCCCA TGTCTGCCTT TGTTGAAAAT
    CACCTGGAAT GGTTCAGGTT CAGTGGTCGT ATTCTTGGCC TTGCTCTCAT TCATCAGTAC
    CTTCTTGATG CTTTCTTCAC AAGGCCGTTC TACAAAGCAC TATTAAGGCT GCCATGTGAT
    CTAAGTGACT TAGAGTACCT GGATGAAGAA TTCCATCAGA GCTTGCAGTG GATGAAAGAT
    AACAACATCA CAGATATCCT GGATCTCACC TTCACAGTGA ATGAGGAGGT GTTTGGACAG
    GTGACAGAGA GAGAGTTGAA ATCTGGAGGG GCAAATACAG CAGTGACAGA AAAGAACAAA
    AAAGAGTACA TTGAGAGAAT GGTTAAATGG CGAGTGGAAC GAGGAGTTGT TCAACAAACA
    GAGGCCCTGG TCCGTGGCTT CTATGAAGTT GTAGACTCAA GGCTTGTCTC TGTTTTTGAT
    GCCAGAGAAC TGGAGCTGGT TATAGCTGGC ACTGCAGAAA TAGATCTCAA TGACTGGCGT
    AACAACACGG AATATCGTGG AGGTTACCAC GATGGACACA TTGTAATACG GTGGTTCTGG
    GCAGCAGTGG AGAGGTTTAA CAATGAACAG AGACTCCGGT TGTTGCAGTT TGTTACTGGA
    ACATCAAGTG TGCCATATGA GGGCTTTGCA GCTCTCCGAG GAAGCAATGG GCTTCGGCGT
    TTCTGTATAG AGAAATGGGG GAAAATAACC TCCCTTCCGA GGGCACATAC ATGTTTCAAC
    CGCTTGGATC TTCCACCTTA CCCCTCATAT TCTATGCTGT ATGAAAAGCT GTTGACAGCT
    GTTGAAGAAA CTAGTACCTT TGGACTTGAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa31695
    Clone ID Related Accession (Same CDS sequence) XM_015281621.2 , XM_015281621.1
    Accession Version XM_015281621.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4413bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAGTTA CAAGAATCCT GCAGTACATG GGCACGAGAT CCTGTAGGCC AATTTGGATT 
    TTTAAAAGCA TCACCAATGA AGATTCAATC CAGGGACAGG GAAATCGGAG GCTGATAAGT
    TTTTCACTGT CAGATTTCCA AGCTTTTGGT CTGAAGAAAG GAATGTTTTT CAATCCAGAT
    CCTTATCTGA AGATTTCCAT CCAGCCTGGG AAACACAGCA TCTTCCCAGC ACTTCCTCAT
    CATGGACAGG AGAAAAGGTC TAAGATCATG TGTAATACAG TTAACCCAAT CTGGCAAGGA
    GAGCAATTCA GCTTTGTCTC ACTGCCCACG GATGTCCTGG AAATAGAAGT TAAAGACAAA
    TTTGCAAAGA GCCGACCAAT CATCAAGCGT TTCCTGGGAA AACTCTCTAT GCCAGTTCAG
    CGTCTCTTGG AAAGACATGC CATAGGGGAC AGGGTTGTAA GCTACACTCT TGGTCGCAGG
    CTTCCTACAG ATCATGTCAG TGGCCAGCTG CAGTTCCGAT TTGAGATAAC TTCTTCCATT
    CATCAAGATG ATGAAGAGGT TACCATTAGT GCAGATCCTG AGCCAGCTGA CCTCCAGGAA
    AGCCCTGTGA ACAACCCCAC AGAGGAAAGC CTCGGTGAGC CTCCATGCAT CAACACAGTG
    ACCTCAGAAA ATACAGTTCC TGAAGAGCTA AATGGATACA GTGTGAACAA TATTGATAGC
    CCAGGGGCTG TCAAACCACC CAGGGAAGTC AACCAGAACA ACATAAATGA AGAGCTAGCA
    GGAGATGAGG AGGTTGATGC CATGCCAGTT CCTGAACCCT TGGAATCCAT CCGAGGAGAA
    GTGCTGCCTT CTTTGAATGC GACAGACCTC ACAGAGCAGC TGGGTCTGCT GGCTTGCTTG
    TCTCCTTCTG AGACTGAAGT TAGGGAAAAT AGCAAAGTCA ATTCTGGTAC TCAGGGAGAT
    GATGGAGTAT TGAACAGTAC AGAAACTTTA GATGTTAGTG AGGTGAGTGC AGATGTATAT
    GCTGACAAAG ACCTAGAGAA GAGTCATGAA GAAGAAGGGG CTTCAGCCCG AGGGGAGGAA
    GACAGCAAGC TACAGCTGAG GACCACAGCA AAGAGGAAAA CAAGGCCATG CTCCCTGCCT
    GTGTCTGAAA TTGAGACCGT TATAGCTTCA GCTTGTGGTG AGCCAGAGAC CCCTCGCACT
    CACTATATAC GAATCCACAA CCTGCTCCAC AGCCTGCCCT CAGCACAGAT GAGCAGTGAT
    GGGGAAGAAG AGCAAGATGC TGGCAATGGT GGGGAGAATG ATGAAGAGTC TACAGTCAAG
    GAAGTGTTGC AGAAGGAGAC AGTCAGTGAA AGTGAGACAA TGGCAGCAGA GCCTCCTCCA
    GAAAATGAGG CCGAAGAGAA CTTCAGCCAG TGCACAGTGC CTCCTGGGAC CTTAGACGAG
    CAACTCTCTG ACTTAAATGA TGGACTGGTG GATGGGGAAA ACCCTAGGAC AGGAAGCGAA
    AGCGAGTCAA GCTCCAGACC CAATGGTGAC AACGAATGTG AGGCGTGCGA CACCTCTTGC
    TACAGCCCCT CCTGCTATAG TACCTCCTGC TACAGTACTT CCTGTTACAG CACCTCTTGC
    TACAGCACCT CCTGCTATGA CAGTAACAAT CGCTTCTCCA GCCATACCCG CTTCTCCTCT
    GTTGACAGTG CAAAGATTTC TGAGAGCACG GTCTTTTCCT CACAGGATGA GGAGGAGGAG
    AATAGTGCTT TTGAATCGGT GCCAGACTCA GTACAGAGCC CTGAGCTGGA CAGGGAGCAA
    GTGGATGGCT CCTCCCAGTG GCCAGATGAA CTAGTAGCTC ATGGTGGGAA TCCACAACTA
    GCCGCAGAAA GTCTAGACAC CCCAATAGCA GGTCCAAGCA GCAGAAGAGA AGGTGATTGT
    CCTTTACTCC ATAATTCTCA GCCAGTCAGC CAACTTCCTT CTCTGAGGCC TGACCATCAT
    CACTACCCAA CTATCGATGA GCCTCTTCCA CCAACTGCAA CATCCTTGAG AAACCTTACC
    CAGTTGGATA ACCAGAGAGC CCTCCCTTTA AAATTATGTG ATGTTCACAG AGAGACTGTA
    TATTGGGAAG CTCGGATAGA CAGCCACGGG AGGGTGTTCT ACGTTGACCA TGTCAACCGA
    ACTACCACAT GGCAGCGCCC CACAGCTGCA GCAACACCAG ATGGGATCCA CAGGTCTGGC
    TCTATCCAGC AAATGGAGCA GCTGAACCGG CGATATCAAA ATATCCAGCG AACTATTGCA
    ACAGAGCGGA CTGAAGATGA TGGTGTAGTA AACAACAGAG TGGAGAGAAT TCCCACTGGA
    GGAGGAAGTG ACTCTGAGGC AGAGCCTTCC CAACCAAGCT CCGAAATTAG GAGGGAAGGT
    TCCCTTTCTC CTGTGAATTC TCAAAAAATC ACCTTATTGC TGCAGTCTCC AGCTGTGAAG
    TTTATCACCA ATCCTGAGTT CTTCACTGTG CTGCATGCAA ACTATAGTGC CTATCGAGTC
    TTCACTAACA GTACCTGCTT GAAGCATATG ATCCTGAAGA TCCGTAGAGA TGCTCGTAAT
    TTTGAGCGCT ATCAGCACAA CAGAGACCTT GTAAATTTTA TCAACATGTT TGCTGACACT
    CGACTAGAGC TTCCTCGTGG CTGGGAAATA AAAACTGACC AGCAGGGCAA GTCTTTCTTC
    GTTGACCACA ACAGTCGTGC TACCACATTC ATAGATCCCA GGATCCCACT GCAGAATGGT
    CGTCTCCCCA ATCACTTGAC TCACAGGCAA CATCTTCAGC GGCTTCGTAG CTACAGTGCT
    GGAGAGGCTT CTGAAGTCTC TCGGAACAGA GGAGTTACTC TGTTGGCCAG ACCAGGCAAT
    AGTCTTGTAA CAGCTATTCG AAACCAGCAT CATTATGAGA CATTACCTCT GGCTTACAAT
    GACAAGATTG TTGCATTTCT ACGCCAACCT AACATTTTTG AGATGCTGCA AGAGCGTCAG
    CCCAGTTTGG CCAGAAATCA TGCACTCAGG GAGAAGATCC ATTATATTCG GACAGAGGGT
    ACACATGGGC TTGAAAAGTT GTCCTGTGAT GCAGATTTAG TAATTCTGCT GAGCCTTTTT
    GAAGAAGATA TCATGTCCTA CATACCACTC CAGGCCACTT TCCATCCTGG TTACAGTTTC
    TCTCCTCGCT GCTCACCCTG CTCATCTCCC CAAAATTCTC CAGGACTGCA GCGAGCAAGT
    GCAAGAGCAC CCTCTCCATA CAGGAGAGAT TTTGAAGCCA AACTACGCAA TTTCTATCGA
    AAACTTGAAG CCAAAGGATA TGGACAAGGT CCAGGTAAAA TTAAGTTAAT TATACGACGT
    GACCACTTAC TGGAAGGCAC CTTCAACCAG GTGATGGCAT ATTCACGCAA GGAGCTCCAG
    CGAAACAAAC TCTACATCAC ATTTGTTGGC GAGGAAGGGC TGGACTACAG TGGCCCCTCT
    CGAGAATTCT TTTTCCTTCT GTCTCAGGAG CTCTTCAATC CATATTACGG GCTGTTTGAG
    TATTCAGCCA ATGACACTTA CACTGTACAG ATTAGCCCCA TGTCTGCCTT TGTTGAAAAT
    CACCTGGAAT GGTTCAGGTT CAGTGGTCGT ATTCTTGGCC TTGCTCTCAT TCATCAGTAC
    CTTCTTGATG CTTTCTTCAC AAGGCCGTTC TACAAAGCAC TATTAAGGCT GCCATGTGAT
    CTAAGTGACT TAGAGTACCT GGATGAAGAA TTCCATCAGA GCTTGCAGTG GATGAAAGAT
    AACAACATCA CAGATATCCT GGATCTCACC TTCACAGTGA ATGAGGAGGT GTTTGGACAG
    GTGACAGAGA GAGAGTTGAA ATCTGGAGGG GCAAATACAG CAGTGACAGA AAAGAACAAA
    AAAGAGTACA TTGAGAGAAT GGTTAAATGG CGAGTGGAAC GAGGAGTTGT TCAACAAACA
    GAGGCCCTGG TCCGTGGCTT CTATGAAGTT GTAGACTCAA GGCTTGTCTC TGTTTTTGAT
    GCCAGAGAAC TGGAGCTGGT TATAGCTGGC ACTGCAGAAA TAGATCTCAA TGACTGGCGT
    AACAACACGG AATATCGTGG AGGTTACCAC GATGGACACA TTGTAATACG GTGGTTCTGG
    GCAGCAGTGG AGAGGTTTAA CAATGAACAG AGACTCCGGT TGTTGCAGTT TGTTACTGGA
    ACATCAAGTG TGCCATATGA GGGCTTTGCA GCTCTCCGAG GAAGCAATGG GCTTCGGCGT
    TTCTGTATAG AGAAATGGGG GAAAATAACC TCCCTTCCGA GGGCACATAC ATGTTTCAAC
    CGCTTGGATC TTCCACCTTA CCCCTCATAT TCTATGCTGT ATGAAAAGCT GTTGACAGCT
    GTTGAAGAAA CTAGTACCTT TGGACTTGAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015137107.1
    CDS49..4461
    Translation

    Target ORF information:

    RefSeq Version XM_015281621.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015281621.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAAGTTA CAAGAATCCT GCAGTACATG GGCACGAGAT CCTGTAGGCC AATTTGGATT 
    TTTAAAAGCA TCACCAATGA AGATTCAATC CAGGGACAGG GAAATCGGAG GCTGATAAGT
    TTTTCACTGT CAGATTTCCA AGCTTTTGGT CTGAAGAAAG GAATGTTTTT CAATCCAGAT
    CCTTATCTGA AGATTTCCAT CCAGCCTGGG AAACACAGCA TCTTCCCAGC ACTTCCTCAT
    CATGGACAGG AGAAAAGGTC TAAGATCATG TGTAATACAG TTAACCCAAT CTGGCAAGGA
    GAGCAATTCA GCTTTGTCTC ACTGCCCACG GATGTCCTGG AAATAGAAGT TAAAGACAAA
    TTTGCAAAGA GCCGACCAAT CATCAAGCGT TTCCTGGGAA AACTCTCTAT GCCAGTTCAG
    CGTCTCTTGG AAAGACATGC CATAGGGGAC AGGGTTGTAA GCTACACTCT TGGTCGCAGG
    CTTCCTACAG ATCATGTCAG TGGCCAGCTG CAGTTCCGAT TTGAGATAAC TTCTTCCATT
    CATCAAGATG ATGAAGAGGT TACCATTAGT GCAGATCCTG AGCCAGCTGA CCTCCAGGAA
    AGCCCTGTGA ACAACCCCAC AGAGGAAAGC CTCGGTGAGC CTCCATGCAT CAACACAGTG
    ACCTCAGAAA ATACAGTTCC TGAAGAGCTA AATGGATACA GTGTGAACAA TATTGATAGC
    CCAGGGGCTG TCAAACCACC CAGGGAAGTC AACCAGAACA ACATAAATGA AGAGCTAGCA
    GGAGATGAGG AGGTTGATGC CATGCCAGTT CCTGAACCCT TGGAATCCAT CCGAGGAGAA
    GTGCTGCCTT CTTTGAATGC GACAGACCTC ACAGAGCAGC TGGGTCTGCT GGCTTGCTTG
    TCTCCTTCTG AGACTGAAGT TAGGGAAAAT AGCAAAGTCA ATTCTGGTAC TCAGGGAGAT
    GATGGAGTAT TGAACAGTAC AGAAACTTTA GATGTTAGTG AGGTGAGTGC AGATGTATAT
    GCTGACAAAG ACCTAGAGAA GAGTCATGAA GAAGAAGGGG CTTCAGCCCG AGGGGAGGAA
    GACAGCAAGC TACAGCTGAG GACCACAGCA AAGAGGAAAA CAAGGCCATG CTCCCTGCCT
    GTGTCTGAAA TTGAGACCGT TATAGCTTCA GCTTGTGGTG AGCCAGAGAC CCCTCGCACT
    CACTATATAC GAATCCACAA CCTGCTCCAC AGCCTGCCCT CAGCACAGAT GAGCAGTGAT
    GGGGAAGAAG AGCAAGATGC TGGCAATGGT GGGGAGAATG ATGAAGAGTC TACAGTCAAG
    GAAGTGTTGC AGAAGGAGAC AGTCAGTGAA AGTGAGACAA TGGCAGCAGA GCCTCCTCCA
    GAAAATGAGG CCGAAGAGAA CTTCAGCCAG TGCACAGTGC CTCCTGGGAC CTTAGACGAG
    CAACTCTCTG ACTTAAATGA TGGACTGGTG GATGGGGAAA ACCCTAGGAC AGGAAGCGAA
    AGCGAGTCAA GCTCCAGACC CAATGGTGAC AACGAATGTG AGGCGTGCGA CACCTCTTGC
    TACAGCCCCT CCTGCTATAG TACCTCCTGC TACAGTACTT CCTGTTACAG CACCTCTTGC
    TACAGCACCT CCTGCTATGA CAGTAACAAT CGCTTCTCCA GCCATACCCG CTTCTCCTCT
    GTTGACAGTG CAAAGATTTC TGAGAGCACG GTCTTTTCCT CACAGGATGA GGAGGAGGAG
    AATAGTGCTT TTGAATCGGT GCCAGACTCA GTACAGAGCC CTGAGCTGGA CAGGGAGCAA
    GTGGATGGCT CCTCCCAGTG GCCAGATGAA CTAGTAGCTC ATGGTGGGAA TCCACAACTA
    GCCGCAGAAA GTCTAGACAC CCCAATAGCA GGTCCAAGCA GCAGAAGAGA AGGTGATTGT
    CCTTTACTCC ATAATTCTCA GCCAGTCAGC CAACTTCCTT CTCTGAGGCC TGACCATCAT
    CACTACCCAA CTATCGATGA GCCTCTTCCA CCAACTGCAA CATCCTTGAG AAACCTTACC
    CAGTTGGATA ACCAGAGAGC CCTCCCTTTA AAATTATGTG ATGTTCACAG AGAGACTGTA
    TATTGGGAAG CTCGGATAGA CAGCCACGGG AGGGTGTTCT ACGTTGACCA TGTCAACCGA
    ACTACCACAT GGCAGCGCCC CACAGCTGCA GCAACACCAG ATGGGATCCA CAGGTCTGGC
    TCTATCCAGC AAATGGAGCA GCTGAACCGG CGATATCAAA ATATCCAGCG AACTATTGCA
    ACAGAGCGGA CTGAAGATGA TGGTGTAGTA AACAACAGAG TGGAGAGAAT TCCCACTGGA
    GGAGGAAGTG ACTCTGAGGC AGAGCCTTCC CAACCAAGCT CCGAAATTAG GAGGGAAGGT
    TCCCTTTCTC CTGTGAATTC TCAAAAAATC ACCTTATTGC TGCAGTCTCC AGCTGTGAAG
    TTTATCACCA ATCCTGAGTT CTTCACTGTG CTGCATGCAA ACTATAGTGC CTATCGAGTC
    TTCACTAACA GTACCTGCTT GAAGCATATG ATCCTGAAGA TCCGTAGAGA TGCTCGTAAT
    TTTGAGCGCT ATCAGCACAA CAGAGACCTT GTAAATTTTA TCAACATGTT TGCTGACACT
    CGACTAGAGC TTCCTCGTGG CTGGGAAATA AAAACTGACC AGCAGGGCAA GTCTTTCTTC
    GTTGACCACA ACAGTCGTGC TACCACATTC ATAGATCCCA GGATCCCACT GCAGAATGGT
    CGTCTCCCCA ATCACTTGAC TCACAGGCAA CATCTTCAGC GGCTTCGTAG CTACAGTGCT
    GGAGAGGCTT CTGAAGTCTC TCGGAACAGA GGAGTTACTC TGTTGGCCAG ACCAGGCAAT
    AGTCTTGTAA CAGCTATTCG AAACCAGCAT CATTATGAGA CATTACCTCT GGCTTACAAT
    GACAAGATTG TTGCATTTCT ACGCCAACCT AACATTTTTG AGATGCTGCA AGAGCGTCAG
    CCCAGTTTGG CCAGAAATCA TGCACTCAGG GAGAAGATCC ATTATATTCG GACAGAGGGT
    ACACATGGGC TTGAAAAGTT GTCCTGTGAT GCAGATTTAG TAATTCTGCT GAGCCTTTTT
    GAAGAAGATA TCATGTCCTA CATACCACTC CAGGCCACTT TCCATCCTGG TTACAGTTTC
    TCTCCTCGCT GCTCACCCTG CTCATCTCCC CAAAATTCTC CAGGACTGCA GCGAGCAAGT
    GCAAGAGCAC CCTCTCCATA CAGGAGAGAT TTTGAAGCCA AACTACGCAA TTTCTATCGA
    AAACTTGAAG CCAAAGGATA TGGACAAGGT CCAGGTAAAA TTAAGTTAAT TATACGACGT
    GACCACTTAC TGGAAGGCAC CTTCAACCAG GTGATGGCAT ATTCACGCAA GGAGCTCCAG
    CGAAACAAAC TCTACATCAC ATTTGTTGGC GAGGAAGGGC TGGACTACAG TGGCCCCTCT
    CGAGAATTCT TTTTCCTTCT GTCTCAGGAG CTCTTCAATC CATATTACGG GCTGTTTGAG
    TATTCAGCCA ATGACACTTA CACTGTACAG ATTAGCCCCA TGTCTGCCTT TGTTGAAAAT
    CACCTGGAAT GGTTCAGGTT CAGTGGTCGT ATTCTTGGCC TTGCTCTCAT TCATCAGTAC
    CTTCTTGATG CTTTCTTCAC AAGGCCGTTC TACAAAGCAC TATTAAGGCT GCCATGTGAT
    CTAAGTGACT TAGAGTACCT GGATGAAGAA TTCCATCAGA GCTTGCAGTG GATGAAAGAT
    AACAACATCA CAGATATCCT GGATCTCACC TTCACAGTGA ATGAGGAGGT GTTTGGACAG
    GTGACAGAGA GAGAGTTGAA ATCTGGAGGG GCAAATACAG CAGTGACAGA AAAGAACAAA
    AAAGAGTACA TTGAGAGAAT GGTTAAATGG CGAGTGGAAC GAGGAGTTGT TCAACAAACA
    GAGGCCCTGG TCCGTGGCTT CTATGAAGTT GTAGACTCAA GGCTTGTCTC TGTTTTTGAT
    GCCAGAGAAC TGGAGCTGGT TATAGCTGGC ACTGCAGAAA TAGATCTCAA TGACTGGCGT
    AACAACACGG AATATCGTGG AGGTTACCAC GATGGACACA TTGTAATACG GTGGTTCTGG
    GCAGCAGTGG AGAGGTTTAA CAATGAACAG AGACTCCGGT TGTTGCAGTT TGTTACTGGA
    ACATCAAGTG TGCCATATGA GGGCTTTGCA GCTCTCCGAG GAAGCAATGG GCTTCGGCGT
    TTCTGTATAG AGAAATGGGG GAAAATAACC TCCCTTCCGA GGGCACATAC ATGTTTCAAC
    CGCTTGGATC TTCCACCTTA CCCCTCATAT TCTATGCTGT ATGAAAAGCT GTTGACAGCT
    GTTGAAGAAA CTAGTACCTT TGGACTTGAA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.