HECW1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECW1
Entrez Gene ID	23072
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Synonyms	NEDL1
General protein information	Preferred Names HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase HECW1 HECT type E3 ubiquitin ligase HECT-type E3 ubiquitin transferase HECW1 NEDD4-like ubiquitin-protein ligase 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p14.1-p13
Disorder MIM:	610384

mRNA and Protein(s)

mRNA	Protein	Name
XM_011515226.2	XP_011513528.1	E3 ubiquitin-protein ligase HECW1 isoform X13
NM_001287059.1	NP_001273988.1	E3 ubiquitin-protein ligase HECW1 isoform b
XM_006715670.3	XP_006715733.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_005249665.4	XP_005249722.1	E3 ubiquitin-protein ligase HECW1 isoform X5
XM_017011885.1	XP_016867374.1	E3 ubiquitin-protein ligase HECW1 isoform X5
XM_011515224.2	XP_011513526.1	E3 ubiquitin-protein ligase HECW1 isoform X9
XM_005249665.3	XP_005249722.1	E3 ubiquitin-protein ligase HECW1 isoform X5
XM_017011883.2	XP_016867372.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_017011884.1	XP_016867373.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_011515220.2	XP_011513522.1	E3 ubiquitin-protein ligase HECW1 isoform X5
XM_017011883.1	XP_016867372.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_017011882.1	XP_016867371.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_011515222.2	XP_011513524.1	E3 ubiquitin-protein ligase HECW1 isoform X9
XM_006715673.3	XP_006715736.1	E3 ubiquitin-protein ligase HECW1 isoform X10
XM_017011889.1	XP_016867378.1	E3 ubiquitin-protein ligase HECW1 isoform X11
NM_015052.4	NP_055867.3	E3 ubiquitin-protein ligase HECW1 isoform a
XM_017011886.1	XP_016867375.1	E3 ubiquitin-protein ligase HECW1 isoform X6
XM_017011887.1	XP_016867376.1	E3 ubiquitin-protein ligase HECW1 isoform X7
XM_011515223.2	XP_011513525.1	E3 ubiquitin-protein ligase HECW1 isoform X9
XM_011515225.2	XP_011513527.2	E3 ubiquitin-protein ligase HECW1 isoform X12
NM_001287059.2	NP_001273988.1	E3 ubiquitin-protein ligase HECW1 isoform b
NM_015052.5	NP_055867.3	E3 ubiquitin-protein ligase HECW1 isoform a
XM_006715671.3	XP_006715734.1	E3 ubiquitin-protein ligase HECW1 isoform X4
XM_011515220.3	XP_011513522.1	E3 ubiquitin-protein ligase HECW1 isoform X5
XM_017011888.1	XP_016867377.1	E3 ubiquitin-protein ligase HECW1 isoform X8
XM_011515226.3	XP_011513528.1	E3 ubiquitin-protein ligase HECW1 isoform X13
XM_017011890.1	XP_016867379.1	E3 ubiquitin-protein ligase HECW1 isoform X14

Pathways from BioSystems

Homologies

Homo sapiens (human)	HECW1	NP_055867.3
Pan troglodytes (chimpanzee)	HECW1	XP_003318432.1
Canis lupus familiaris (dog)	HECW1	XP_005630981.1
Danio rerio (zebrafish)	hecw1b	NP_001139236.1
Mus musculus (house mouse)	Hecw1	NP_001074817.3
Xenopus tropicalis (tropical clawed frog)	hecw1	NP_001123750.1
	HECW1	XP_001096775.1
Rattus norvegicus (Norway rat)	Hecw1	NP_001099587.1
Gallus gallus (chicken)	HECW1	XP_004939648.1

Gene Ontology
Bibliography

Related articles in PubMed

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.
Inaba H, Goto H, Kasahara K, Kumamoto K, Yonemura S, Inoko A, Yamano S, Wanibuchi H, He D, Goshima N, Kiyono T, Hirotsune S, Inagaki M
The Journal of cell biology212(4)409-23(2016 Feb)

Meta-analysis of genome-wide association studies in African Americans provides insights into the genetic architecture of type 2 diabetes.
Ng MC, Shriner D, Chen BH, Li J, Chen WM, Guo X, Liu J, Bielinski SJ, Yanek LR, Nalls MA, Comeau ME, Rasmussen-Torvik LJ, Jensen RA, Evans DS, Sun YV, An P, Patel SR, Lu Y, Long J, Armstrong LL, Wagenknecht L, Yang L, Snively BM, Palmer ND, Mudgal P, Langefeld CD, Keene KL, Freedman BI, Mychaleckyj JC, Nayak U, Raffel LJ, Goodarzi MO, Chen YD, Taylor HA Jr, Correa A, Sims M, Couper D, Pankow JS, Boerwinkle E, Adeyemo A, Doumatey A, Chen G, Mathias RA, Vaidya D, Singleton AB, Zonderman AB, Igo RP Jr, Sedor JR, , Kabagambe EK, Siscovick DS, McKnight B, Rice K, Liu Y, Hsueh WC, Zhao W, Bielak LF, Kraja A, Province MA, Bottinger EP, Gottesman O, Cai Q, Zheng W, Blot WJ, Lowe WL, Pacheco JA, Crawford DC, , , Grundberg E, , Rich SS, Hayes MG, Shu XO, Loos RJ, Borecki IB, Peyser PA, Cummings SR, Psaty BM, Fornage M, Iyengar SK, Evans MK, Becker DM, Kao WH, Wilson JG, Rotter JI, Sale MM, Liu S, Rotimi CN, Bowden DW,
PLoS genetics10(8)e1004517(2014 Aug)

Genome-wide association study of the rate of cognitive decline in Alzheimer's disease.
Sherva R, Tripodis Y, Bennett DA, Chibnik LB, Crane PK, de Jager PL, Farrer LA, Saykin AJ, Shulman JM, Naj A, Green RC, , ,
Alzheimer's & dementia : the journal of the Alzheimer's Association10(1)45-52(2014 Jan)

A genome-wide association study identifies new susceptibility loci for esophageal adenocarcinoma and Barrett's esophagus.
Levine DM, Ek WE, Zhang R, Liu X, Onstad L, Sather C, Lao-Sirieix P, Gammon MD, Corley DA, Shaheen NJ, Bird NC, Hardie LJ, Murray LJ, Reid BJ, Chow WH, Risch HA, Nyr?n O, Ye W, Liu G, Romero Y, Bernstein L, Wu AH, Casson AG, Chanock SJ, Harrington P, Caldas I, Debiram-Beecham I, Caldas C, Hayward NK, Pharoah PD, Fitzgerald RC, Macgregor S, Whiteman DC, Vaughan TL
Nature genetics45(12)1487-93(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ndel1 acts as a novel upstream regulator of the trichoplein-Aurora A pathway to inhibit primary cilia assembly.
Title: Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.

These findings suggest that the human NEDL1 transgenic mice might develop Amyotrophic lateral sclerosis-like symptoms, showing signs of motor abnormalities, accompanied with significant reduction in muscle strength.
Title: Muscle atrophy and motor neuron degeneration in human NEDL1 transgenic mice.

These findings suggest that RNF43 is associated with p53-mediated apoptosis in collaboration with NEDL1 in colorectal carcinogenesis.
Title: RNF43 interacts with NEDL1 and regulates p53-mediated transcription.

Ndel1 regulates Dyn2 GTPase activity and impacts GluR1-containing membranes distribution in a manner reminiscent of Dyn2.
Title: The cytoskeletal protein Ndel1 regulates dynamin 2 GTPase activity.

functional interaction of NEDL1 with p53 might contribute to the induction of apoptosis in cancerous cells bearing wild-type p53.
Title: A novel HECT-type E3 ubiquitin protein ligase NEDL1 enhances the p53-mediated apoptotic cell death in its catalytic activity-independent manner.

The following HECW1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11752	NM_015052.5 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu11752	XM_017011886.1 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu12837	NM_001287059.2 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu12837	XM_017011889.1 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu41113	XM_006715670.3 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu41114	XM_006715671.3 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu41115	XM_011515220.3 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu41115	XM_005249665.4 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu41115	XM_017011885.1 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu41116	XM_006715673.3 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu65383	XM_011515222.2 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu65383	XM_011515223.2 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu65383	XM_011515224.2 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu65385	XM_011515226.3 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu97114	XM_017011882.1 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu97114	XM_017011883.2 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu97115	XM_017011884.1 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu97116	XM_017011887.1 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu97117	XM_017011888.1 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu97118	XM_011515225.2 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu97119	XM_017011890.1 Latest version!	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
View Old Accession Versions >>
OHu41115	XM_005249665.3	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu97114	XM_017011883.1	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu12837	NM_001287059.1	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu11752	NM_015052.4	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu41115	XM_011515220.2	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu65385	XM_011515226.2	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECW1 ( NM_015052.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055867.3 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11752
Clone ID Related Accession (Same CDS sequence)	NM_015052.4 , XM_017011886.1 , NM_015052.5
Accession Version	NM_015052.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC151227.1, AK294918.1 and AC011738.4. On Nov 23, 2013 this sequence version replaced NM_015052.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: BC151227.1, AB002320.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT ACATGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGATGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAGTTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGTCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACGGAGA AAAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACTGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055867.3
CDS	606..5426
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	NM_015052.4
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015052.4

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT ACATGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGATGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAGTTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGTCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACGGAGA AAAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACTGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( NM_015052.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055867.3 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant 1, mRNA.

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CloneID	OHu11752
Clone ID Related Accession (Same CDS sequence)	NM_015052.4 , XM_017011886.1 , NM_015052.5
Accession Version	NM_015052.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC151227.1, AK294918.1 and AC011738.4. On Nov 24, 2018 this sequence version replaced NM_015052.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC151227.1, AB002320.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465403 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000395891.7/ ENSP00000379228.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT ACATGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGATGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAGTTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGTCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACGGAGA AAAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACTGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055867.3
CDS	558..5378
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	NM_015052.5
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015052.5

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT ACATGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGATGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAGTTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGTCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACGGAGA AAAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACTGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017011886.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867375.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA.

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CloneID	OHu11752
Clone ID Related Accession (Same CDS sequence)	NM_015052.4 , XM_017011886.1 , NM_015052.5
Accession Version	XM_017011886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT ACATGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGATGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAGTTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGTCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACGGAGA AAAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACTGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867375.1
CDS	390..5210
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017011886.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011886.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT ACATGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGATGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAGTTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGTCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACGGAGA AAAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACTGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( NM_001287059.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273988.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12837
Clone ID Related Accession (Same CDS sequence)	NM_001287059.1 , XM_017011889.1 , NM_001287059.2
Accession Version	NM_001287059.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC151227.1, AK294918.1, AC004692.2 and AC011738.4. On Nov 23, 2013 this sequence version replaced XM_005249667.1. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: AK294918.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGAC 
TGGGAAGCTC GAATTGACAG CCACGGGCGG GTCTTTTATG TGGACCACGT GAACCGCACA 
ACCACCTGGC AGCGTCCGAC GGCAGCAGCC ACCCCGGATG GCATGCGGAG ATCGGGGTCC 
ATCCAGCAGA TGGAGCAACT CAACAGGCGG TATCAAAACA TTCAGCGAAC CATTGCAACA 
GAGAGGTCCG AAGAAGATTC TGGCAGCCAA AGCTGCGAGC AAGCCCCAGC AGGAGGAGGC 
GGAGGTGGAG GGAGTGACTC AGAAGCCGAA TCTTCCCAGT CCAGCTTAGA TCTAAGGAGA 
GAAGGGTCAC TTTCTCCAGT GAACTCACAA AAAATCACCT TGCTGCTGCA GTCCCCAGCG 
GTCAAGTTCA TCACCAACCC CGAGTTCTTC ACTGTGCTAC ATGCCAATTA TAGTGCCTAC 
CGAGTCTTCA CCAGTAGCAC CTGCTTAAAG CACATGATTC TGAAAGTCCG ACGGGATGCT 
CGCAATTTTG AACGCTACCA GCACAACCGG GACTTGGTGA ATTTCATCAA CATGTTCGCA 
GACACTCGGC TGGAACTGCC CCGGGGCTGG GAGATCAAAA CGGACCAGCA GGGAAAGTCT 
TTTTTCGTGG ACCACAACAG TCGAGCTACC ACTTTCATTG ACCCCCGAAT CCCTCTTCAG 
AACGGTCGTC TTCCCAATCA TCTAACTCAC CGACAGCACC TCCAGAGGCT CCGAAGTTAC 
AGCGCTGGAG AGGCCTCAGA AGTTTCTAGA AACAGAGGAG CCTCTTTACT GGCCAGGCCA 
GGACACAGCT TAGTAGCTGC TATTCGAAGC CAACATCAAC ATGAGTCATT GCCACTGGCA 
TATAATGACA AGATTGTGGC ATTTCTTCGC CAGCCAAACA TTTTTGAAAT GCTGCAAGAG 
CGTCAGCCAA GCTTAGCAAG AAACCACACA CTCAGGGAGA AAATCCATTA CATTCGGACT 
GAGGGTAATC ACGGGCTTGA GAAGTTGTCC TGTGATGCGG ATCTGGTCAT TTTGCTGAGT 
CTCTTTGAAG AAGAGATTAT GTCCTACGTC CCCCTGCAGG CTGCCTTCCA CCCTGGGTAT 
AGCTTCTCTC CCCGATGTTC ACCCTGTTCT TCACCTCAGA ACTCCCCAGG TTTACAGAGA 
GCCAGTGCAA GAGCCCCTTC CCCCTACCGA AGAGACTTTG AGGCCAAGCT CCGCAATTTC 
TACAGAAAAC TGGAAGCCAA AGGATTTGGT CAGGGTCCGG GGAAAATTAA GCTCATTATT 
CGCCGGGATC ATTTGTTGGA GGGAACCTTC AATCAGGTGA TGGCCTATTC GCGGAAAGAG 
CTCCAGCGAA ACAAGCTCTA CGTCACCTTT GTTGGAGAGG AGGGCCTGGA CTACAGTGGC 
CCCTCGCGGG AGTTCTTCTT CCTTCTGTCT CAGGAGCTCT TCAACCCTTA CTATGGACTC 
TTTGAGTACT CGGCAAATGA TACTTACACG GTGCAGATCA GCCCCATGTC CGCATTTGTA 
GAAAACCATC TTGAGTGGTT CAGGTTTAGC GGTCGCATCC TGGGTCTGGC TCTGATCCAT 
CAGTACCTTC TTGACGCTTT CTTCACGAGG CCCTTCTACA AGGCACTCCT GAGACTGCCC 
TGTGATTTGA GTGACCTGGA ATATTTGGAT GAGGAATTCC ACCAGAGTTT GCAGTGGATG 
AAGGACAACA ACATCACAGA CATCTTAGAC CTCACTTTCA CTGTTAATGA AGAGGTTTTT 
GGACAGGTCA CGGAAAGGGA GTTGAAGTCT GGAGGAGCCA ACACACAGGT GACGGAGAAA 
AACAAGAAGG AGTACATCGA GCGCATGGTG AAGTGGCGGG TGGAGCGCGG CGTGGTACAG 
CAGACCGAGG CGCTGGTGCG CGGCTTCTAC GAGGTTGTAG ACTCGAGGCT GGTGTCCGTG 
TTTGATGCCA GGGAGCTGGA GCTGGTGATA GCTGGCACCG CGGAAATCGA CCTAAATGAC 
TGGCGGAATA ACACTGAGTA CCGGGGAGGT TACCACGATG GGCATCTTGT GATCCGCTGG 
TTCTGGGCTG CGGTGGAGCG CTTCAATAAT GAGCAGAGGC TGAGATTACT GCAGTTTGTC 
ACGGGAACAT CCAGCGTGCC CTACGAAGGC TTCGCAGCCC TCCGTGGGAG CAATGGGCTT 
CGGCGCTTCT GCATAGAGAA ATGGGGGAAA ATTACTTCTC TCCCCAGGGC ACACACATGC 
TTCAACCGAC TGGATCTTCC ACCGTATCCC TCGTACTCCA TGTTGTATGA AAAGCTGTTA 
ACAGCAGTAG AGGAAACCAG CACCTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273988.1
CDS	371..5089
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	NM_001287059.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287059.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGAC 
TGGGAAGCTC GAATTGACAG CCACGGGCGG GTCTTTTATG TGGACCACGT GAACCGCACA 
ACCACCTGGC AGCGTCCGAC GGCAGCAGCC ACCCCGGATG GCATGCGGAG ATCGGGGTCC 
ATCCAGCAGA TGGAGCAACT CAACAGGCGG TATCAAAACA TTCAGCGAAC CATTGCAACA 
GAGAGGTCCG AAGAAGATTC TGGCAGCCAA AGCTGCGAGC AAGCCCCAGC AGGAGGAGGC 
GGAGGTGGAG GGAGTGACTC AGAAGCCGAA TCTTCCCAGT CCAGCTTAGA TCTAAGGAGA 
GAAGGGTCAC TTTCTCCAGT GAACTCACAA AAAATCACCT TGCTGCTGCA GTCCCCAGCG 
GTCAAGTTCA TCACCAACCC CGAGTTCTTC ACTGTGCTAC ATGCCAATTA TAGTGCCTAC 
CGAGTCTTCA CCAGTAGCAC CTGCTTAAAG CACATGATTC TGAAAGTCCG ACGGGATGCT 
CGCAATTTTG AACGCTACCA GCACAACCGG GACTTGGTGA ATTTCATCAA CATGTTCGCA 
GACACTCGGC TGGAACTGCC CCGGGGCTGG GAGATCAAAA CGGACCAGCA GGGAAAGTCT 
TTTTTCGTGG ACCACAACAG TCGAGCTACC ACTTTCATTG ACCCCCGAAT CCCTCTTCAG 
AACGGTCGTC TTCCCAATCA TCTAACTCAC CGACAGCACC TCCAGAGGCT CCGAAGTTAC 
AGCGCTGGAG AGGCCTCAGA AGTTTCTAGA AACAGAGGAG CCTCTTTACT GGCCAGGCCA 
GGACACAGCT TAGTAGCTGC TATTCGAAGC CAACATCAAC ATGAGTCATT GCCACTGGCA 
TATAATGACA AGATTGTGGC ATTTCTTCGC CAGCCAAACA TTTTTGAAAT GCTGCAAGAG 
CGTCAGCCAA GCTTAGCAAG AAACCACACA CTCAGGGAGA AAATCCATTA CATTCGGACT 
GAGGGTAATC ACGGGCTTGA GAAGTTGTCC TGTGATGCGG ATCTGGTCAT TTTGCTGAGT 
CTCTTTGAAG AAGAGATTAT GTCCTACGTC CCCCTGCAGG CTGCCTTCCA CCCTGGGTAT 
AGCTTCTCTC CCCGATGTTC ACCCTGTTCT TCACCTCAGA ACTCCCCAGG TTTACAGAGA 
GCCAGTGCAA GAGCCCCTTC CCCCTACCGA AGAGACTTTG AGGCCAAGCT CCGCAATTTC 
TACAGAAAAC TGGAAGCCAA AGGATTTGGT CAGGGTCCGG GGAAAATTAA GCTCATTATT 
CGCCGGGATC ATTTGTTGGA GGGAACCTTC AATCAGGTGA TGGCCTATTC GCGGAAAGAG 
CTCCAGCGAA ACAAGCTCTA CGTCACCTTT GTTGGAGAGG AGGGCCTGGA CTACAGTGGC 
CCCTCGCGGG AGTTCTTCTT CCTTCTGTCT CAGGAGCTCT TCAACCCTTA CTATGGACTC 
TTTGAGTACT CGGCAAATGA TACTTACACG GTGCAGATCA GCCCCATGTC CGCATTTGTA 
GAAAACCATC TTGAGTGGTT CAGGTTTAGC GGTCGCATCC TGGGTCTGGC TCTGATCCAT 
CAGTACCTTC TTGACGCTTT CTTCACGAGG CCCTTCTACA AGGCACTCCT GAGACTGCCC 
TGTGATTTGA GTGACCTGGA ATATTTGGAT GAGGAATTCC ACCAGAGTTT GCAGTGGATG 
AAGGACAACA ACATCACAGA CATCTTAGAC CTCACTTTCA CTGTTAATGA AGAGGTTTTT 
GGACAGGTCA CGGAAAGGGA GTTGAAGTCT GGAGGAGCCA ACACACAGGT GACGGAGAAA 
AACAAGAAGG AGTACATCGA GCGCATGGTG AAGTGGCGGG TGGAGCGCGG CGTGGTACAG 
CAGACCGAGG CGCTGGTGCG CGGCTTCTAC GAGGTTGTAG ACTCGAGGCT GGTGTCCGTG 
TTTGATGCCA GGGAGCTGGA GCTGGTGATA GCTGGCACCG CGGAAATCGA CCTAAATGAC 
TGGCGGAATA ACACTGAGTA CCGGGGAGGT TACCACGATG GGCATCTTGT GATCCGCTGG 
TTCTGGGCTG CGGTGGAGCG CTTCAATAAT GAGCAGAGGC TGAGATTACT GCAGTTTGTC 
ACGGGAACAT CCAGCGTGCC CTACGAAGGC TTCGCAGCCC TCCGTGGGAG CAATGGGCTT 
CGGCGCTTCT GCATAGAGAA ATGGGGGAAA ATTACTTCTC TCCCCAGGGC ACACACATGC 
TTCAACCGAC TGGATCTTCC ACCGTATCCC TCGTACTCCA TGTTGTATGA AAAGCTGTTA 
ACAGCAGTAG AGGAAACCAG CACCTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( NM_001287059.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273988.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12837
Clone ID Related Accession (Same CDS sequence)	NM_001287059.1 , XM_017011889.1 , NM_001287059.2
Accession Version	NM_001287059.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK294918.1, AC004692.2, AC011738.4 and BC151227.1. On May 31, 2019 this sequence version replaced NM_001287059.1. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK294918.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGAC 
TGGGAAGCTC GAATTGACAG CCACGGGCGG GTCTTTTATG TGGACCACGT GAACCGCACA 
ACCACCTGGC AGCGTCCGAC GGCAGCAGCC ACCCCGGATG GCATGCGGAG ATCGGGGTCC 
ATCCAGCAGA TGGAGCAACT CAACAGGCGG TATCAAAACA TTCAGCGAAC CATTGCAACA 
GAGAGGTCCG AAGAAGATTC TGGCAGCCAA AGCTGCGAGC AAGCCCCAGC AGGAGGAGGC 
GGAGGTGGAG GGAGTGACTC AGAAGCCGAA TCTTCCCAGT CCAGCTTAGA TCTAAGGAGA 
GAAGGGTCAC TTTCTCCAGT GAACTCACAA AAAATCACCT TGCTGCTGCA GTCCCCAGCG 
GTCAAGTTCA TCACCAACCC CGAGTTCTTC ACTGTGCTAC ATGCCAATTA TAGTGCCTAC 
CGAGTCTTCA CCAGTAGCAC CTGCTTAAAG CACATGATTC TGAAAGTCCG ACGGGATGCT 
CGCAATTTTG AACGCTACCA GCACAACCGG GACTTGGTGA ATTTCATCAA CATGTTCGCA 
GACACTCGGC TGGAACTGCC CCGGGGCTGG GAGATCAAAA CGGACCAGCA GGGAAAGTCT 
TTTTTCGTGG ACCACAACAG TCGAGCTACC ACTTTCATTG ACCCCCGAAT CCCTCTTCAG 
AACGGTCGTC TTCCCAATCA TCTAACTCAC CGACAGCACC TCCAGAGGCT CCGAAGTTAC 
AGCGCTGGAG AGGCCTCAGA AGTTTCTAGA AACAGAGGAG CCTCTTTACT GGCCAGGCCA 
GGACACAGCT TAGTAGCTGC TATTCGAAGC CAACATCAAC ATGAGTCATT GCCACTGGCA 
TATAATGACA AGATTGTGGC ATTTCTTCGC CAGCCAAACA TTTTTGAAAT GCTGCAAGAG 
CGTCAGCCAA GCTTAGCAAG AAACCACACA CTCAGGGAGA AAATCCATTA CATTCGGACT 
GAGGGTAATC ACGGGCTTGA GAAGTTGTCC TGTGATGCGG ATCTGGTCAT TTTGCTGAGT 
CTCTTTGAAG AAGAGATTAT GTCCTACGTC CCCCTGCAGG CTGCCTTCCA CCCTGGGTAT 
AGCTTCTCTC CCCGATGTTC ACCCTGTTCT TCACCTCAGA ACTCCCCAGG TTTACAGAGA 
GCCAGTGCAA GAGCCCCTTC CCCCTACCGA AGAGACTTTG AGGCCAAGCT CCGCAATTTC 
TACAGAAAAC TGGAAGCCAA AGGATTTGGT CAGGGTCCGG GGAAAATTAA GCTCATTATT 
CGCCGGGATC ATTTGTTGGA GGGAACCTTC AATCAGGTGA TGGCCTATTC GCGGAAAGAG 
CTCCAGCGAA ACAAGCTCTA CGTCACCTTT GTTGGAGAGG AGGGCCTGGA CTACAGTGGC 
CCCTCGCGGG AGTTCTTCTT CCTTCTGTCT CAGGAGCTCT TCAACCCTTA CTATGGACTC 
TTTGAGTACT CGGCAAATGA TACTTACACG GTGCAGATCA GCCCCATGTC CGCATTTGTA 
GAAAACCATC TTGAGTGGTT CAGGTTTAGC GGTCGCATCC TGGGTCTGGC TCTGATCCAT 
CAGTACCTTC TTGACGCTTT CTTCACGAGG CCCTTCTACA AGGCACTCCT GAGACTGCCC 
TGTGATTTGA GTGACCTGGA ATATTTGGAT GAGGAATTCC ACCAGAGTTT GCAGTGGATG 
AAGGACAACA ACATCACAGA CATCTTAGAC CTCACTTTCA CTGTTAATGA AGAGGTTTTT 
GGACAGGTCA CGGAAAGGGA GTTGAAGTCT GGAGGAGCCA ACACACAGGT GACGGAGAAA 
AACAAGAAGG AGTACATCGA GCGCATGGTG AAGTGGCGGG TGGAGCGCGG CGTGGTACAG 
CAGACCGAGG CGCTGGTGCG CGGCTTCTAC GAGGTTGTAG ACTCGAGGCT GGTGTCCGTG 
TTTGATGCCA GGGAGCTGGA GCTGGTGATA GCTGGCACCG CGGAAATCGA CCTAAATGAC 
TGGCGGAATA ACACTGAGTA CCGGGGAGGT TACCACGATG GGCATCTTGT GATCCGCTGG 
TTCTGGGCTG CGGTGGAGCG CTTCAATAAT GAGCAGAGGC TGAGATTACT GCAGTTTGTC 
ACGGGAACAT CCAGCGTGCC CTACGAAGGC TTCGCAGCCC TCCGTGGGAG CAATGGGCTT 
CGGCGCTTCT GCATAGAGAA ATGGGGGAAA ATTACTTCTC TCCCCAGGGC ACACACATGC 
TTCAACCGAC TGGATCTTCC ACCGTATCCC TCGTACTCCA TGTTGTATGA AAAGCTGTTA 
ACAGCAGTAG AGGAAACCAG CACCTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273988.1
CDS	323..5041
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	NM_001287059.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287059.2

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGAC 
TGGGAAGCTC GAATTGACAG CCACGGGCGG GTCTTTTATG TGGACCACGT GAACCGCACA 
ACCACCTGGC AGCGTCCGAC GGCAGCAGCC ACCCCGGATG GCATGCGGAG ATCGGGGTCC 
ATCCAGCAGA TGGAGCAACT CAACAGGCGG TATCAAAACA TTCAGCGAAC CATTGCAACA 
GAGAGGTCCG AAGAAGATTC TGGCAGCCAA AGCTGCGAGC AAGCCCCAGC AGGAGGAGGC 
GGAGGTGGAG GGAGTGACTC AGAAGCCGAA TCTTCCCAGT CCAGCTTAGA TCTAAGGAGA 
GAAGGGTCAC TTTCTCCAGT GAACTCACAA AAAATCACCT TGCTGCTGCA GTCCCCAGCG 
GTCAAGTTCA TCACCAACCC CGAGTTCTTC ACTGTGCTAC ATGCCAATTA TAGTGCCTAC 
CGAGTCTTCA CCAGTAGCAC CTGCTTAAAG CACATGATTC TGAAAGTCCG ACGGGATGCT 
CGCAATTTTG AACGCTACCA GCACAACCGG GACTTGGTGA ATTTCATCAA CATGTTCGCA 
GACACTCGGC TGGAACTGCC CCGGGGCTGG GAGATCAAAA CGGACCAGCA GGGAAAGTCT 
TTTTTCGTGG ACCACAACAG TCGAGCTACC ACTTTCATTG ACCCCCGAAT CCCTCTTCAG 
AACGGTCGTC TTCCCAATCA TCTAACTCAC CGACAGCACC TCCAGAGGCT CCGAAGTTAC 
AGCGCTGGAG AGGCCTCAGA AGTTTCTAGA AACAGAGGAG CCTCTTTACT GGCCAGGCCA 
GGACACAGCT TAGTAGCTGC TATTCGAAGC CAACATCAAC ATGAGTCATT GCCACTGGCA 
TATAATGACA AGATTGTGGC ATTTCTTCGC CAGCCAAACA TTTTTGAAAT GCTGCAAGAG 
CGTCAGCCAA GCTTAGCAAG AAACCACACA CTCAGGGAGA AAATCCATTA CATTCGGACT 
GAGGGTAATC ACGGGCTTGA GAAGTTGTCC TGTGATGCGG ATCTGGTCAT TTTGCTGAGT 
CTCTTTGAAG AAGAGATTAT GTCCTACGTC CCCCTGCAGG CTGCCTTCCA CCCTGGGTAT 
AGCTTCTCTC CCCGATGTTC ACCCTGTTCT TCACCTCAGA ACTCCCCAGG TTTACAGAGA 
GCCAGTGCAA GAGCCCCTTC CCCCTACCGA AGAGACTTTG AGGCCAAGCT CCGCAATTTC 
TACAGAAAAC TGGAAGCCAA AGGATTTGGT CAGGGTCCGG GGAAAATTAA GCTCATTATT 
CGCCGGGATC ATTTGTTGGA GGGAACCTTC AATCAGGTGA TGGCCTATTC GCGGAAAGAG 
CTCCAGCGAA ACAAGCTCTA CGTCACCTTT GTTGGAGAGG AGGGCCTGGA CTACAGTGGC 
CCCTCGCGGG AGTTCTTCTT CCTTCTGTCT CAGGAGCTCT TCAACCCTTA CTATGGACTC 
TTTGAGTACT CGGCAAATGA TACTTACACG GTGCAGATCA GCCCCATGTC CGCATTTGTA 
GAAAACCATC TTGAGTGGTT CAGGTTTAGC GGTCGCATCC TGGGTCTGGC TCTGATCCAT 
CAGTACCTTC TTGACGCTTT CTTCACGAGG CCCTTCTACA AGGCACTCCT GAGACTGCCC 
TGTGATTTGA GTGACCTGGA ATATTTGGAT GAGGAATTCC ACCAGAGTTT GCAGTGGATG 
AAGGACAACA ACATCACAGA CATCTTAGAC CTCACTTTCA CTGTTAATGA AGAGGTTTTT 
GGACAGGTCA CGGAAAGGGA GTTGAAGTCT GGAGGAGCCA ACACACAGGT GACGGAGAAA 
AACAAGAAGG AGTACATCGA GCGCATGGTG AAGTGGCGGG TGGAGCGCGG CGTGGTACAG 
CAGACCGAGG CGCTGGTGCG CGGCTTCTAC GAGGTTGTAG ACTCGAGGCT GGTGTCCGTG 
TTTGATGCCA GGGAGCTGGA GCTGGTGATA GCTGGCACCG CGGAAATCGA CCTAAATGAC 
TGGCGGAATA ACACTGAGTA CCGGGGAGGT TACCACGATG GGCATCTTGT GATCCGCTGG 
TTCTGGGCTG CGGTGGAGCG CTTCAATAAT GAGCAGAGGC TGAGATTACT GCAGTTTGTC 
ACGGGAACAT CCAGCGTGCC CTACGAAGGC TTCGCAGCCC TCCGTGGGAG CAATGGGCTT 
CGGCGCTTCT GCATAGAGAA ATGGGGGAAA ATTACTTCTC TCCCCAGGGC ACACACATGC 
TTCAACCGAC TGGATCTTCC ACCGTATCCC TCGTACTCCA TGTTGTATGA AAAGCTGTTA 
ACAGCAGTAG AGGAAACCAG CACCTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017011889.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867378.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X16, mRNA.

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CloneID	OHu12837
Clone ID Related Accession (Same CDS sequence)	NM_001287059.1 , XM_017011889.1 , NM_001287059.2
Accession Version	XM_017011889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGAC 
TGGGAAGCTC GAATTGACAG CCACGGGCGG GTCTTTTATG TGGACCACGT GAACCGCACA 
ACCACCTGGC AGCGTCCGAC GGCAGCAGCC ACCCCGGATG GCATGCGGAG ATCGGGGTCC 
ATCCAGCAGA TGGAGCAACT CAACAGGCGG TATCAAAACA TTCAGCGAAC CATTGCAACA 
GAGAGGTCCG AAGAAGATTC TGGCAGCCAA AGCTGCGAGC AAGCCCCAGC AGGAGGAGGC 
GGAGGTGGAG GGAGTGACTC AGAAGCCGAA TCTTCCCAGT CCAGCTTAGA TCTAAGGAGA 
GAAGGGTCAC TTTCTCCAGT GAACTCACAA AAAATCACCT TGCTGCTGCA GTCCCCAGCG 
GTCAAGTTCA TCACCAACCC CGAGTTCTTC ACTGTGCTAC ATGCCAATTA TAGTGCCTAC 
CGAGTCTTCA CCAGTAGCAC CTGCTTAAAG CACATGATTC TGAAAGTCCG ACGGGATGCT 
CGCAATTTTG AACGCTACCA GCACAACCGG GACTTGGTGA ATTTCATCAA CATGTTCGCA 
GACACTCGGC TGGAACTGCC CCGGGGCTGG GAGATCAAAA CGGACCAGCA GGGAAAGTCT 
TTTTTCGTGG ACCACAACAG TCGAGCTACC ACTTTCATTG ACCCCCGAAT CCCTCTTCAG 
AACGGTCGTC TTCCCAATCA TCTAACTCAC CGACAGCACC TCCAGAGGCT CCGAAGTTAC 
AGCGCTGGAG AGGCCTCAGA AGTTTCTAGA AACAGAGGAG CCTCTTTACT GGCCAGGCCA 
GGACACAGCT TAGTAGCTGC TATTCGAAGC CAACATCAAC ATGAGTCATT GCCACTGGCA 
TATAATGACA AGATTGTGGC ATTTCTTCGC CAGCCAAACA TTTTTGAAAT GCTGCAAGAG 
CGTCAGCCAA GCTTAGCAAG AAACCACACA CTCAGGGAGA AAATCCATTA CATTCGGACT 
GAGGGTAATC ACGGGCTTGA GAAGTTGTCC TGTGATGCGG ATCTGGTCAT TTTGCTGAGT 
CTCTTTGAAG AAGAGATTAT GTCCTACGTC CCCCTGCAGG CTGCCTTCCA CCCTGGGTAT 
AGCTTCTCTC CCCGATGTTC ACCCTGTTCT TCACCTCAGA ACTCCCCAGG TTTACAGAGA 
GCCAGTGCAA GAGCCCCTTC CCCCTACCGA AGAGACTTTG AGGCCAAGCT CCGCAATTTC 
TACAGAAAAC TGGAAGCCAA AGGATTTGGT CAGGGTCCGG GGAAAATTAA GCTCATTATT 
CGCCGGGATC ATTTGTTGGA GGGAACCTTC AATCAGGTGA TGGCCTATTC GCGGAAAGAG 
CTCCAGCGAA ACAAGCTCTA CGTCACCTTT GTTGGAGAGG AGGGCCTGGA CTACAGTGGC 
CCCTCGCGGG AGTTCTTCTT CCTTCTGTCT CAGGAGCTCT TCAACCCTTA CTATGGACTC 
TTTGAGTACT CGGCAAATGA TACTTACACG GTGCAGATCA GCCCCATGTC CGCATTTGTA 
GAAAACCATC TTGAGTGGTT CAGGTTTAGC GGTCGCATCC TGGGTCTGGC TCTGATCCAT 
CAGTACCTTC TTGACGCTTT CTTCACGAGG CCCTTCTACA AGGCACTCCT GAGACTGCCC 
TGTGATTTGA GTGACCTGGA ATATTTGGAT GAGGAATTCC ACCAGAGTTT GCAGTGGATG 
AAGGACAACA ACATCACAGA CATCTTAGAC CTCACTTTCA CTGTTAATGA AGAGGTTTTT 
GGACAGGTCA CGGAAAGGGA GTTGAAGTCT GGAGGAGCCA ACACACAGGT GACGGAGAAA 
AACAAGAAGG AGTACATCGA GCGCATGGTG AAGTGGCGGG TGGAGCGCGG CGTGGTACAG 
CAGACCGAGG CGCTGGTGCG CGGCTTCTAC GAGGTTGTAG ACTCGAGGCT GGTGTCCGTG 
TTTGATGCCA GGGAGCTGGA GCTGGTGATA GCTGGCACCG CGGAAATCGA CCTAAATGAC 
TGGCGGAATA ACACTGAGTA CCGGGGAGGT TACCACGATG GGCATCTTGT GATCCGCTGG 
TTCTGGGCTG CGGTGGAGCG CTTCAATAAT GAGCAGAGGC TGAGATTACT GCAGTTTGTC 
ACGGGAACAT CCAGCGTGCC CTACGAAGGC TTCGCAGCCC TCCGTGGGAG CAATGGGCTT 
CGGCGCTTCT GCATAGAGAA ATGGGGGAAA ATTACTTCTC TCCCCAGGGC ACACACATGC 
TTCAACCGAC TGGATCTTCC ACCGTATCCC TCGTACTCCA TGTTGTATGA AAAGCTGTTA 
ACAGCAGTAG AGGAAACCAG CACCTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867378.1
CDS	682..5400
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017011889.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011889.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGAC 
TGGGAAGCTC GAATTGACAG CCACGGGCGG GTCTTTTATG TGGACCACGT GAACCGCACA 
ACCACCTGGC AGCGTCCGAC GGCAGCAGCC ACCCCGGATG GCATGCGGAG ATCGGGGTCC 
ATCCAGCAGA TGGAGCAACT CAACAGGCGG TATCAAAACA TTCAGCGAAC CATTGCAACA 
GAGAGGTCCG AAGAAGATTC TGGCAGCCAA AGCTGCGAGC AAGCCCCAGC AGGAGGAGGC 
GGAGGTGGAG GGAGTGACTC AGAAGCCGAA TCTTCCCAGT CCAGCTTAGA TCTAAGGAGA 
GAAGGGTCAC TTTCTCCAGT GAACTCACAA AAAATCACCT TGCTGCTGCA GTCCCCAGCG 
GTCAAGTTCA TCACCAACCC CGAGTTCTTC ACTGTGCTAC ATGCCAATTA TAGTGCCTAC 
CGAGTCTTCA CCAGTAGCAC CTGCTTAAAG CACATGATTC TGAAAGTCCG ACGGGATGCT 
CGCAATTTTG AACGCTACCA GCACAACCGG GACTTGGTGA ATTTCATCAA CATGTTCGCA 
GACACTCGGC TGGAACTGCC CCGGGGCTGG GAGATCAAAA CGGACCAGCA GGGAAAGTCT 
TTTTTCGTGG ACCACAACAG TCGAGCTACC ACTTTCATTG ACCCCCGAAT CCCTCTTCAG 
AACGGTCGTC TTCCCAATCA TCTAACTCAC CGACAGCACC TCCAGAGGCT CCGAAGTTAC 
AGCGCTGGAG AGGCCTCAGA AGTTTCTAGA AACAGAGGAG CCTCTTTACT GGCCAGGCCA 
GGACACAGCT TAGTAGCTGC TATTCGAAGC CAACATCAAC ATGAGTCATT GCCACTGGCA 
TATAATGACA AGATTGTGGC ATTTCTTCGC CAGCCAAACA TTTTTGAAAT GCTGCAAGAG 
CGTCAGCCAA GCTTAGCAAG AAACCACACA CTCAGGGAGA AAATCCATTA CATTCGGACT 
GAGGGTAATC ACGGGCTTGA GAAGTTGTCC TGTGATGCGG ATCTGGTCAT TTTGCTGAGT 
CTCTTTGAAG AAGAGATTAT GTCCTACGTC CCCCTGCAGG CTGCCTTCCA CCCTGGGTAT 
AGCTTCTCTC CCCGATGTTC ACCCTGTTCT TCACCTCAGA ACTCCCCAGG TTTACAGAGA 
GCCAGTGCAA GAGCCCCTTC CCCCTACCGA AGAGACTTTG AGGCCAAGCT CCGCAATTTC 
TACAGAAAAC TGGAAGCCAA AGGATTTGGT CAGGGTCCGG GGAAAATTAA GCTCATTATT 
CGCCGGGATC ATTTGTTGGA GGGAACCTTC AATCAGGTGA TGGCCTATTC GCGGAAAGAG 
CTCCAGCGAA ACAAGCTCTA CGTCACCTTT GTTGGAGAGG AGGGCCTGGA CTACAGTGGC 
CCCTCGCGGG AGTTCTTCTT CCTTCTGTCT CAGGAGCTCT TCAACCCTTA CTATGGACTC 
TTTGAGTACT CGGCAAATGA TACTTACACG GTGCAGATCA GCCCCATGTC CGCATTTGTA 
GAAAACCATC TTGAGTGGTT CAGGTTTAGC GGTCGCATCC TGGGTCTGGC TCTGATCCAT 
CAGTACCTTC TTGACGCTTT CTTCACGAGG CCCTTCTACA AGGCACTCCT GAGACTGCCC 
TGTGATTTGA GTGACCTGGA ATATTTGGAT GAGGAATTCC ACCAGAGTTT GCAGTGGATG 
AAGGACAACA ACATCACAGA CATCTTAGAC CTCACTTTCA CTGTTAATGA AGAGGTTTTT 
GGACAGGTCA CGGAAAGGGA GTTGAAGTCT GGAGGAGCCA ACACACAGGT GACGGAGAAA 
AACAAGAAGG AGTACATCGA GCGCATGGTG AAGTGGCGGG TGGAGCGCGG CGTGGTACAG 
CAGACCGAGG CGCTGGTGCG CGGCTTCTAC GAGGTTGTAG ACTCGAGGCT GGTGTCCGTG 
TTTGATGCCA GGGAGCTGGA GCTGGTGATA GCTGGCACCG CGGAAATCGA CCTAAATGAC 
TGGCGGAATA ACACTGAGTA CCGGGGAGGT TACCACGATG GGCATCTTGT GATCCGCTGG 
TTCTGGGCTG CGGTGGAGCG CTTCAATAAT GAGCAGAGGC TGAGATTACT GCAGTTTGTC 
ACGGGAACAT CCAGCGTGCC CTACGAAGGC TTCGCAGCCC TCCGTGGGAG CAATGGGCTT 
CGGCGCTTCT GCATAGAGAA ATGGGGGAAA ATTACTTCTC TCCCCAGGGC ACACACATGC 
TTCAACCGAC TGGATCTTCC ACCGTATCCC TCGTACTCCA TGTTGTATGA AAAGCTGTTA 
ACAGCAGTAG AGGAAACCAG CACCTTTGGA CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_006715670.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006715733.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

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CloneID	OHu41113
Clone ID Related Accession (Same CDS sequence)	XM_006715670.3
Accession Version	XM_006715670.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006715670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCG TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACCGAG 
CCTGAGTCAG CCCAAATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGGT CTGAGGCACC AGAGTCCTCT GAGAGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC CCGATGGTCC AGGGAACCAA AGCATAGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCACGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGAGCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCCAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCA TCAGCACTGC TGCTGGAAGA CGGTGAAGCC 
CCAGCCAGCA CCAAGGAGGA GCCCTTGGAG GAGGAAGCAA CGACCCAGAG CCGGGCTGGA 
AGGGAAGAAG AGGAGAAGGA GCAGGAGGAG GAGGGAGATG TGTCTACCCT GGAGCAGGGA 
GAGGGCAGGC TGCAGCTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCCGAGC TGGAGACGGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CCGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC GGTGGCCGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CACCCTGGCC ACTCCGGGGG CCACTTCCCC 
AGCCTGGCCA ATGGCGCGGC CCAGGATGGC GACACGCACC CCAGCACCGG GAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGACGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCGT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TGGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGACGAC 
GAGGAGGAGG AGAACAGCGC GTTCGAGTCG GTACCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGTGGAAA GAAGCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGGTGAAT GTCCTATACT CCATAATTCC CAGCCAGTAA GCCAGCTTCC TTCCCTGAGG 
CCTGAACATC ATCACTACCC AACAATCGAT GAGCCTCTTC CACCAAACTG GGAAGCTCGA 
ATTGACAGCC ACGGGCGGGT CTTTTATGTG GACCACGTGA ACCGCACAAC CACCTGGCAG 
CGTCCGACGG CAGCAGCCAC CCCGGATGGC ATGCGGAGAT CGGGGTCCAT CCAGCAGATG 
GAGCAACTCA ACAGGCGGTA TCAAAACATT CAGCGAACCA TTGCAACAGA GAGGTCCGAA 
GAAGATTCTG GCAGCCAAAG CTGCGAGCAA GCCCCAGCAG GAGGAGGCGG AGGTGGAGGG 
AGTGACTCAG AAGCCGAATC TTCCCAGTCC AGCTTAGATC TAAGGAGAGA AGGGTCACTT 
TCTCCAGTGA ACTCACAAAA AATCACCTTG CTGCTGCAGT CCCCAGCGGT CAAGTTCATC 
ACCAACCCCG AGTTCTTCAC TGTGCTACAT GCCAATTATA GTGCCTACCG AGTCTTCACC 
AGTAGCACCT GCTTAAAGCA CATGATTCTG AAAGTCCGAC GGGATGCTCG CAATTTTGAA 
CGCTACCAGC ACAACCGGGA CTTGGTGAAT TTCATCAACA TGTTCGCAGA CACTCGGCTG 
GAACTGCCCC GGGGCTGGGA GATCAAAACG GACCAGCAGG GAAAGTCTTT TTTCGTGGAC 
CACAACAGTC GAGCTACCAC TTTCATTGAC CCCCGAATCC CTCTTCAGAA CGGTCGTCTT 
CCCAATCATC TAACTCACCG ACAGCACCTC CAGAGGCTCC GAAGTTACAG CGCTGGAGAG 
GCCTCAGAAG TTTCTAGAAA CAGAGGAGCC TCTTTACTGG CCAGGCCAGG ACACAGCTTA 
GTAGCTGCTA TTCGAAGCCA ACATCAACAT GAGTCATTGC CACTGGCATA TAATGACAAG 
ATTGTGGCAT TTCTTCGCCA GCCAAACATT TTTGAAATGC TGCAAGAGCG TCAGCCAAGC 
TTAGCAAGAA ACCACACACT CAGGGAGAAA ATCCATTACA TTCGGACTGA GGGTAATCAC 
GGGCTTGAGA AGTTGTCCTG TGATGCGGAT CTGGTCATTT TGCTGAGTCT CTTTGAAGAA 
GAGATTATGT CCTACGTCCC CCTGCAGGCT GCCTTCCACC CTGGGTATAG CTTCTCTCCC 
CGATGTTCAC CCTGTTCTTC ACCTCAGAAC TCCCCAGGTT TACAGAGAGC CAGTGCAAGA 
GCCCCTTCCC CCTACCGAAG AGACTTTGAG GCCAAGCTCC GCAATTTCTA CAGAAAACTG 
GAAGCCAAAG GATTTGGTCA GGGTCCGGGG AAAATTAAGC TCATTATTCG CCGGGATCAT 
TTGTTGGAGG GAACCTTCAA TCAGGTGATG GCCTATTCGC GGAAAGAGCT CCAGCGAAAC 
AAGCTCTACG TCACCTTTGT TGGAGAGGAG GGCCTGGACT ACAGTGGCCC CTCGCGGGAG 
TTCTTCTTCC TTCTGTCTCA GGAGCTCTTC AACCCTTACT ATGGACTCTT TGAGTACTCG 
GCAAATGATA CTTACACGGT GCAGATCAGC CCCATGTCCG CATTTGTAGA AAACCATCTT 
GAGTGGTTCA GGTTTAGCGG TCGCATCCTG GGTCTGGCTC TGATCCATCA GTACCTTCTT 
GACGCTTTCT TCACGAGGCC CTTCTACAAG GCACTCCTGA GACTGCCCTG TGATTTGAGT 
GACCTGGAAT ATTTGGATGA GGAATTCCAC CAGAGTTTGC AGTGGATGAA GGACAACAAC 
ATCACAGACA TCTTAGACCT CACTTTCACT GTTAATGAAG AGGTTTTTGG ACAGGTCACG 
GAAAGGGAGT TGAAGTCTGG AGGAGCCAAC ACACAGGTGA CGGAGAAAAA CAAGAAGGAG 
TACATCGAGC GCATGGTGAA GTGGCGGGTG GAGCGCGGCG TGGTACAGCA GACCGAGGCG 
CTGGTGCGCG GCTTCTACGA GGTTGTAGAC TCGAGGCTGG TGTCCGTGTT TGATGCCAGG 
GAGCTGGAGC TGGTGATAGC TGGCACCGCG GAAATCGACC TAAATGACTG GCGGAATAAC 
ACTGAGTACC GGGGAGGTTA CCACGATGGG CATCTTGTGA TCCGCTGGTT CTGGGCTGCG 
GTGGAGCGCT TCAATAATGA GCAGAGGCTG AGATTACTGC AGTTTGTCAC GGGAACATCC 
AGCGTGCCCT ACGAAGGCTT CGCAGCCCTC CGTGGGAGCA ATGGGCTTCG GCGCTTCTGC 
ATAGAGAAAT GGGGGAAAAT TACTTCTCTC CCCAGGGCAC ACACATGCTT CAACCGACTG 
GATCTTCCAC CGTATCCCTC GTACTCCATG TTGTATGAAA AGCTGTTAAC AGCAGTAGAG 
GAAACCAGCA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006715733.1
CDS	280..5106
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006715670.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006715670.3

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCG TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGCA GATGATGAGG AGATTTCCCT GAGTACCGAG 
CCTGAGTCAG CCCAAATTCA GGACAGCCCC ATGAACAACC TGATGGAAAG CGGCAGTGGG 
GAACCTCGGT CTGAGGCACC AGAGTCCTCT GAGAGCTGGA AGCCAGAGCA GCTGGGTGAG 
GGCAGTGTCC CCGATGGTCC AGGGAACCAA AGCATAGAGC TTTCCAGACC AGCTGAGGAA 
GCAGCAGTCA TCACGGAGGC AGGAGACCAG GGCATGGTCT CTGTGGGACC TGAAGGGGCT 
GGGGAGCTCC TGGCCCAGGT GCAAAAGGAC ATCCAGCCTG CCCCCAGTGC AGAAGAGCTG 
GCCGAGCAGC TGGACCTGGG TGAGGAGGCA TCAGCACTGC TGCTGGAAGA CGGTGAAGCC 
CCAGCCAGCA CCAAGGAGGA GCCCTTGGAG GAGGAAGCAA CGACCCAGAG CCGGGCTGGA 
AGGGAAGAAG AGGAGAAGGA GCAGGAGGAG GAGGGAGATG TGTCTACCCT GGAGCAGGGA 
GAGGGCAGGC TGCAGCTGCG GGCCTCGGTG AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT 
GTGTCCGAGC TGGAGACGGT GATCGCGTCA GCCTGCGGGG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CCGCCCAGGG CGGCAGCGCG 
GCAGAGGAGG AGGACGGCGC GGAGGAGGAG TCCACCCTCA AGGACTCCTC GGAGAAGGAT 
GGGCTCAGCG AGGTGGACAC GGTGGCCGCT GACCCGTCTG CCCTGGAAGA GGACAGAGAA 
GAGCCCGAGG GGGCTACTCC AGGCACGGCG CACCCTGGCC ACTCCGGGGG CCACTTCCCC 
AGCCTGGCCA ATGGCGCGGC CCAGGATGGC GACACGCACC CCAGCACCGG GAGCGAGAGC 
GACTCCAGCC CCAGGCAAGG CGGGGACCAC AGTTGCGAGG GCTGTGACGC GTCCTGCTGC 
AGCCCCTCGT GCTACAGCTC CTCGTGCTAC AGCACGTCCT GCTACAGCAG CTCGTGCTAC 
AGCGCCTCGT GCTACAGCCC CTCCTGCTAC AACGGCAACA GGTTCGCCAG CCACACGCGC 
TTCTCCTCCG TGGACAGCGC CAAGATCTCC GAGAGCACGG TCTTCTCCTC GCAAGACGAC 
GAGGAGGAGG AGAACAGCGC GTTCGAGTCG GTACCCGACT CCATGCAGAG CCCTGAGCTG 
GACCCGGAGT CCACGAACGG CGCTGGGCCG TGGCAAGACG AGCTGGCCGC CCCTAGCGGG 
CACGTGGAAA GAAGCCCGGA AGGTCTGGAA TCCCCCGTGG CAGGTCCAAG CAATCGGAGA 
GAAGGTGAAT GTCCTATACT CCATAATTCC CAGCCAGTAA GCCAGCTTCC TTCCCTGAGG 
CCTGAACATC ATCACTACCC AACAATCGAT GAGCCTCTTC CACCAAACTG GGAAGCTCGA 
ATTGACAGCC ACGGGCGGGT CTTTTATGTG GACCACGTGA ACCGCACAAC CACCTGGCAG 
CGTCCGACGG CAGCAGCCAC CCCGGATGGC ATGCGGAGAT CGGGGTCCAT CCAGCAGATG 
GAGCAACTCA ACAGGCGGTA TCAAAACATT CAGCGAACCA TTGCAACAGA GAGGTCCGAA 
GAAGATTCTG GCAGCCAAAG CTGCGAGCAA GCCCCAGCAG GAGGAGGCGG AGGTGGAGGG 
AGTGACTCAG AAGCCGAATC TTCCCAGTCC AGCTTAGATC TAAGGAGAGA AGGGTCACTT 
TCTCCAGTGA ACTCACAAAA AATCACCTTG CTGCTGCAGT CCCCAGCGGT CAAGTTCATC 
ACCAACCCCG AGTTCTTCAC TGTGCTACAT GCCAATTATA GTGCCTACCG AGTCTTCACC 
AGTAGCACCT GCTTAAAGCA CATGATTCTG AAAGTCCGAC GGGATGCTCG CAATTTTGAA 
CGCTACCAGC ACAACCGGGA CTTGGTGAAT TTCATCAACA TGTTCGCAGA CACTCGGCTG 
GAACTGCCCC GGGGCTGGGA GATCAAAACG GACCAGCAGG GAAAGTCTTT TTTCGTGGAC 
CACAACAGTC GAGCTACCAC TTTCATTGAC CCCCGAATCC CTCTTCAGAA CGGTCGTCTT 
CCCAATCATC TAACTCACCG ACAGCACCTC CAGAGGCTCC GAAGTTACAG CGCTGGAGAG 
GCCTCAGAAG TTTCTAGAAA CAGAGGAGCC TCTTTACTGG CCAGGCCAGG ACACAGCTTA 
GTAGCTGCTA TTCGAAGCCA ACATCAACAT GAGTCATTGC CACTGGCATA TAATGACAAG 
ATTGTGGCAT TTCTTCGCCA GCCAAACATT TTTGAAATGC TGCAAGAGCG TCAGCCAAGC 
TTAGCAAGAA ACCACACACT CAGGGAGAAA ATCCATTACA TTCGGACTGA GGGTAATCAC 
GGGCTTGAGA AGTTGTCCTG TGATGCGGAT CTGGTCATTT TGCTGAGTCT CTTTGAAGAA 
GAGATTATGT CCTACGTCCC CCTGCAGGCT GCCTTCCACC CTGGGTATAG CTTCTCTCCC 
CGATGTTCAC CCTGTTCTTC ACCTCAGAAC TCCCCAGGTT TACAGAGAGC CAGTGCAAGA 
GCCCCTTCCC CCTACCGAAG AGACTTTGAG GCCAAGCTCC GCAATTTCTA CAGAAAACTG 
GAAGCCAAAG GATTTGGTCA GGGTCCGGGG AAAATTAAGC TCATTATTCG CCGGGATCAT 
TTGTTGGAGG GAACCTTCAA TCAGGTGATG GCCTATTCGC GGAAAGAGCT CCAGCGAAAC 
AAGCTCTACG TCACCTTTGT TGGAGAGGAG GGCCTGGACT ACAGTGGCCC CTCGCGGGAG 
TTCTTCTTCC TTCTGTCTCA GGAGCTCTTC AACCCTTACT ATGGACTCTT TGAGTACTCG 
GCAAATGATA CTTACACGGT GCAGATCAGC CCCATGTCCG CATTTGTAGA AAACCATCTT 
GAGTGGTTCA GGTTTAGCGG TCGCATCCTG GGTCTGGCTC TGATCCATCA GTACCTTCTT 
GACGCTTTCT TCACGAGGCC CTTCTACAAG GCACTCCTGA GACTGCCCTG TGATTTGAGT 
GACCTGGAAT ATTTGGATGA GGAATTCCAC CAGAGTTTGC AGTGGATGAA GGACAACAAC 
ATCACAGACA TCTTAGACCT CACTTTCACT GTTAATGAAG AGGTTTTTGG ACAGGTCACG 
GAAAGGGAGT TGAAGTCTGG AGGAGCCAAC ACACAGGTGA CGGAGAAAAA CAAGAAGGAG 
TACATCGAGC GCATGGTGAA GTGGCGGGTG GAGCGCGGCG TGGTACAGCA GACCGAGGCG 
CTGGTGCGCG GCTTCTACGA GGTTGTAGAC TCGAGGCTGG TGTCCGTGTT TGATGCCAGG 
GAGCTGGAGC TGGTGATAGC TGGCACCGCG GAAATCGACC TAAATGACTG GCGGAATAAC 
ACTGAGTACC GGGGAGGTTA CCACGATGGG CATCTTGTGA TCCGCTGGTT CTGGGCTGCG 
GTGGAGCGCT TCAATAATGA GCAGAGGCTG AGATTACTGC AGTTTGTCAC GGGAACATCC 
AGCGTGCCCT ACGAAGGCTT CGCAGCCCTC CGTGGGAGCA ATGGGCTTCG GCGCTTCTGC 
ATAGAGAAAT GGGGGAAAAT TACTTCTCTC CCCAGGGCAC ACACATGCTT CAACCGACTG 
GATCTTCCAC CGTATCCCTC GTACTCCATG TTGTATGAAA AGCTGTTAAC AGCAGTAGAG 
GAAACCAGCA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_006715671.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006715734.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

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CloneID	OHu41114
Clone ID Related Accession (Same CDS sequence)	XM_006715671.3
Accession Version	XM_006715671.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006715671.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCG TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATAGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCATCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACGA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGGCCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAG 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTACATGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGA 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAGTTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGT CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAAAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACT 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006715734.1
CDS	280..5103
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006715671.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006715671.3

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCG TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATAGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCATCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACGA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGGCCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAG 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTACATGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGA 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAGTTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGT CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAAAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACT 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_005249665.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005249722.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

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CloneID	OHu41115
Clone ID Related Accession (Same CDS sequence)	XM_005249665.4 , XM_017011885.1 , XM_005249665.3 , XM_011515220.2 , XM_011515220.3
Accession Version	XM_005249665.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005249665.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATAGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCATCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACGA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGGCCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAG 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTACATGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGA 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAGTTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGT CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAAAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACT 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005249722.1
CDS	348..5171
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_005249665.4
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249665.4

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATAGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCATCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACGA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGGCCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAG 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTACATGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGA 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAGTTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGT CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAAAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACT 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017011885.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867374.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu41115
Clone ID Related Accession (Same CDS sequence)	XM_005249665.4 , XM_017011885.1 , XM_005249665.3 , XM_011515220.2 , XM_011515220.3
Accession Version	XM_017011885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATAGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCATCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACGA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGGCCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAG 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTACATGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGA 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAGTTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGT CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAAAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACT 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867374.1
CDS	558..5381
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017011885.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011885.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATAGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCATCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACGA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGGCCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAG 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTACATGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGA 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAGTTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGT CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAAAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACT 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_005249665.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005249722.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

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CloneID	OHu41115
Clone ID Related Accession (Same CDS sequence)	XM_005249665.4 , XM_017011885.1 , XM_005249665.3 , XM_011515220.2 , XM_011515220.3
Accession Version	XM_005249665.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005249665.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATAGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCATCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACGA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGGCCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAG 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTACATGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGA 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAGTTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGT CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAAAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACT 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005249722.1
CDS	385..5208
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_005249665.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249665.3

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATAGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCATCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACGA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGGCCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAG 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTACATGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGA 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAGTTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGT CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAAAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACT 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_011515220.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513522.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.

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CloneID	OHu41115
Clone ID Related Accession (Same CDS sequence)	XM_005249665.4 , XM_017011885.1 , XM_005249665.3 , XM_011515220.2 , XM_011515220.3
Accession Version	XM_011515220.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515220.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATAGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCATCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACGA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGGCCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAG 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTACATGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGA 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAGTTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGT CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAAAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACT 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513522.1
CDS	176..4999
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_011515220.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515220.2

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATAGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCATCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACGA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGGCCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAG 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTACATGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGA 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAGTTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGT CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAAAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACT 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_011515220.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513522.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.

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CloneID	OHu41115
Clone ID Related Accession (Same CDS sequence)	XM_005249665.4 , XM_017011885.1 , XM_005249665.3 , XM_011515220.2 , XM_011515220.3
Accession Version	XM_011515220.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011515220.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATAGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCATCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACGA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGGCCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAG 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTACATGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGA 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAGTTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGT CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAAAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACT 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513522.1
CDS	177..5000
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_011515220.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515220.3

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATAGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCATCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACGA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGGCCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAG 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GGTGAATGTC CTATACTCCA TAATTCCCAG CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT 
GAACATCATC ACTACCCAAC AATCGATGAG CCTCTTCCAC CAAACTGGGA AGCTCGAATT 
GACAGCCACG GGCGGGTCTT TTATGTGGAC CACGTGAACC GCACAACCAC CTGGCAGCGT 
CCGACGGCAG CAGCCACCCC GGATGGCATG CGGAGATCGG GGTCCATCCA GCAGATGGAG 
CAACTCAACA GGCGGTATCA AAACATTCAG CGAACCATTG CAACAGAGAG GTCCGAAGAA 
GATTCTGGCA GCCAAAGCTG CGAGCAAGCC CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT 
GACTCAGAAG CCGAATCTTC CCAGTCCAGC TTAGATCTAA GGAGAGAAGG GTCACTTTCT 
CCAGTGAACT CACAAAAAAT CACCTTGCTG CTGCAGTCCC CAGCGGTCAA GTTCATCACC 
AACCCCGAGT TCTTCACTGT GCTACATGCC AATTATAGTG CCTACCGAGT CTTCACCAGT 
AGCACCTGCT TAAAGCACAT GATTCTGAAA GTCCGACGGG ATGCTCGCAA TTTTGAACGC 
TACCAGCACA ACCGGGACTT GGTGAATTTC ATCAACATGT TCGCAGACAC TCGGCTGGAA 
CTGCCCCGGG GCTGGGAGAT CAAAACGGAC CAGCAGGGAA AGTCTTTTTT CGTGGACCAC 
AACAGTCGAG CTACCACTTT CATTGACCCC CGAATCCCTC TTCAGAACGG TCGTCTTCCC 
AATCATCTAA CTCACCGACA GCACCTCCAG AGGCTCCGAA GTTACAGCGC TGGAGAGGCC 
TCAGAAGTTT CTAGAAACAG AGGAGCCTCT TTACTGGCCA GGCCAGGACA CAGCTTAGTA 
GCTGCTATTC GAAGCCAACA TCAACATGAG TCATTGCCAC TGGCATATAA TGACAAGATT 
GTGGCATTTC TTCGCCAGCC AAACATTTTT GAAATGCTGC AAGAGCGTCA GCCAAGCTTA 
GCAAGAAACC ACACACTCAG GGAGAAAATC CATTACATTC GGACTGAGGG TAATCACGGG 
CTTGAGAAGT TGTCCTGTGA TGCGGATCTG GTCATTTTGC TGAGTCTCTT TGAAGAAGAG 
ATTATGTCCT ACGTCCCCCT GCAGGCTGCC TTCCACCCTG GGTATAGCTT CTCTCCCCGA 
TGTTCACCCT GTTCTTCACC TCAGAACTCC CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC 
CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC AAGCTCCGCA ATTTCTACAG AAAACTGGAA 
GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA ATTAAGCTCA TTATTCGCCG GGATCATTTG 
TTGGAGGGAA CCTTCAATCA GGTGATGGCC TATTCGCGGA AAGAGCTCCA GCGAAACAAG 
CTCTACGTCA CCTTTGTTGG AGAGGAGGGC CTGGACTACA GTGGCCCCTC GCGGGAGTTC 
TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC CCTTACTATG GACTCTTTGA GTACTCGGCA 
AATGATACTT ACACGGTGCA GATCAGCCCC ATGTCCGCAT TTGTAGAAAA CCATCTTGAG 
TGGTTCAGGT TTAGCGGTCG CATCCTGGGT CTGGCTCTGA TCCATCAGTA CCTTCTTGAC 
GCTTTCTTCA CGAGGCCCTT CTACAAGGCA CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC 
CTGGAATATT TGGATGAGGA ATTCCACCAG AGTTTGCAGT GGATGAAGGA CAACAACATC 
ACAGACATCT TAGACCTCAC TTTCACTGTT AATGAAGAGG TTTTTGGACA GGTCACGGAA 
AGGGAGTTGA AGTCTGGAGG AGCCAACACA CAGGTGACGG AGAAAAACAA GAAGGAGTAC 
ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG CGCGGCGTGG TACAGCAGAC CGAGGCGCTG 
GTGCGCGGCT TCTACGAGGT TGTAGACTCG AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG 
CTGGAGCTGG TGATAGCTGG CACCGCGGAA ATCGACCTAA ATGACTGGCG GAATAACACT 
GAGTACCGGG GAGGTTACCA CGATGGGCAT CTTGTGATCC GCTGGTTCTG GGCTGCGGTG 
GAGCGCTTCA ATAATGAGCA GAGGCTGAGA TTACTGCAGT TTGTCACGGG AACATCCAGC 
GTGCCCTACG AAGGCTTCGC AGCCCTCCGT GGGAGCAATG GGCTTCGGCG CTTCTGCATA 
GAGAAATGGG GGAAAATTAC TTCTCTCCCC AGGGCACACA CATGCTTCAA CCGACTGGAT 
CTTCCACCGT ATCCCTCGTA CTCCATGTTG TATGAAAAGC TGTTAACAGC AGTAGAGGAA 
ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_006715673.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006715736.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHu41116
Clone ID Related Accession (Same CDS sequence)	XM_006715673.3
Accession Version	XM_006715673.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4722bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006715673.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCG TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATAGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCATCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACGA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGGCCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAG 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GACTGGGAAG CTCGAATTGA CAGCCACGGG CGGGTCTTTT ATGTGGACCA CGTGAACCGC 
ACAACCACCT GGCAGCGTCC GACGGCAGCA GCCACCCCGG ATGGCATGCG GAGATCGGGG 
TCCATCCAGC AGATGGAGCA ACTCAACAGG CGGTATCAAA ACATTCAGCG AACCATTGCA 
ACAGAGAGGT CCGAAGAAGA TTCTGGCAGC CAAAGCTGCG AGCAAGCCCC AGCAGGAGGA 
GGCGGAGGTG GAGGGAGTGA CTCAGAAGCC GAATCTTCCC AGTCCAGCTT AGATCTAAGG 
AGAGAAGGGT CACTTTCTCC AGTGAACTCA CAAAAAATCA CCTTGCTGCT GCAGTCCCCA 
GCGGTCAAGT TCATCACCAA CCCCGAGTTC TTCACTGTGC TACATGCCAA TTATAGTGCC 
TACCGAGTCT TCACCAGTAG CACCTGCTTA AAGCACATGA TTCTGAAAGT CCGACGGGAT 
GCTCGCAATT TTGAACGCTA CCAGCACAAC CGGGACTTGG TGAATTTCAT CAACATGTTC 
GCAGACACTC GGCTGGAACT GCCCCGGGGC TGGGAGATCA AAACGGACCA GCAGGGAAAG 
TCTTTTTTCG TGGACCACAA CAGTCGAGCT ACCACTTTCA TTGACCCCCG AATCCCTCTT 
CAGAACGGTC GTCTTCCCAA TCATCTAACT CACCGACAGC ACCTCCAGAG GCTCCGAAGT 
TACAGCGCTG GAGAGGCCTC AGAAGTTTCT AGAAACAGAG GAGCCTCTTT ACTGGCCAGG 
CCAGGACACA GCTTAGTAGC TGCTATTCGA AGCCAACATC AACATGAGTC ATTGCCACTG 
GCATATAATG ACAAGATTGT GGCATTTCTT CGCCAGCCAA ACATTTTTGA AATGCTGCAA 
GAGCGTCAGC CAAGCTTAGC AAGAAACCAC ACACTCAGGG AGAAAATCCA TTACATTCGG 
ACTGAGGGTA ATCACGGGCT TGAGAAGTTG TCCTGTGATG CGGATCTGGT CATTTTGCTG 
AGTCTCTTTG AAGAAGAGAT TATGTCCTAC GTCCCCCTGC AGGCTGCCTT CCACCCTGGG 
TATAGCTTCT CTCCCCGATG TTCACCCTGT TCTTCACCTC AGAACTCCCC AGGTTTACAG 
AGAGCCAGTG CAAGAGCCCC TTCCCCCTAC CGAAGAGACT TTGAGGCCAA GCTCCGCAAT 
TTCTACAGAA AACTGGAAGC CAAAGGATTT GGTCAGGGTC CGGGGAAAAT TAAGCTCATT 
ATTCGCCGGG ATCATTTGTT GGAGGGAACC TTCAATCAGG TGATGGCCTA TTCGCGGAAA 
GAGCTCCAGC GAAACAAGCT CTACGTCACC TTTGTTGGAG AGGAGGGCCT GGACTACAGT 
GGCCCCTCGC GGGAGTTCTT CTTCCTTCTG TCTCAGGAGC TCTTCAACCC TTACTATGGA 
CTCTTTGAGT ACTCGGCAAA TGATACTTAC ACGGTGCAGA TCAGCCCCAT GTCCGCATTT 
GTAGAAAACC ATCTTGAGTG GTTCAGGTTT AGCGGTCGCA TCCTGGGTCT GGCTCTGATC 
CATCAGTACC TTCTTGACGC TTTCTTCACG AGGCCCTTCT ACAAGGCACT CCTGAGACTG 
CCCTGTGATT TGAGTGACCT GGAATATTTG GATGAGGAAT TCCACCAGAG TTTGCAGTGG 
ATGAAGGACA ACAACATCAC AGACATCTTA GACCTCACTT TCACTGTTAA TGAAGAGGTT 
TTTGGACAGG TCACGGAAAG GGAGTTGAAG TCTGGAGGAG CCAACACACA GGTGACGGAG 
AAAAACAAGA AGGAGTACAT CGAGCGCATG GTGAAGTGGC GGGTGGAGCG CGGCGTGGTA 
CAGCAGACCG AGGCGCTGGT GCGCGGCTTC TACGAGGTTG TAGACTCGAG GCTGGTGTCC 
GTGTTTGATG CCAGGGAGCT GGAGCTGGTG ATAGCTGGCA CCGCGGAAAT CGACCTAAAT 
GACTGGCGGA ATAACACTGA GTACCGGGGA GGTTACCACG ATGGGCATCT TGTGATCCGC 
TGGTTCTGGG CTGCGGTGGA GCGCTTCAAT AATGAGCAGA GGCTGAGATT ACTGCAGTTT 
GTCACGGGAA CATCCAGCGT GCCCTACGAA GGCTTCGCAG CCCTCCGTGG GAGCAATGGG 
CTTCGGCGCT TCTGCATAGA GAAATGGGGG AAAATTACTT CTCTCCCCAG GGCACACACA 
TGCTTCAACC GACTGGATCT TCCACCGTAT CCCTCGTACT CCATGTTGTA TGAAAAGCTG 
TTAACAGCAG TAGAGGAAAC CAGCACCTTT GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006715736.1
CDS	280..5001
Translation	MICAQARAAKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_006715673.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006715673.3

ORF Insert Sequence:

ATGATCTGTG CCCAGGCCAG GGCTGCCAAG AATCTGTACC AGAACAGGTT TTTAGGCCTG 
GCCGCCATGG CGTCTCCTTC TAGAAACTCC CAGAGCCGAC GCCGGTGCAA GGAGCCGCTC 
CGATACAGCT ACAACCCCGA CCAGTTCCAC AACATGGACC TCAGGGGCGG CCCCCACGAT 
GGCGTCACCA TTCCCCGCTC CACCAGCGAC ACTGACCTGG TCACCTCGGA CAGCCGCTCC 
ACGCTCATGG TCAGCAGCTC CTACTATTCC ATCGGGCACT CTCAGGACCT GGTCATCCAC 
TGGGACATAA AGGAGGAAGT GGACGCTGGG GACTGGATTG GCATGTACCT CATTGATGAG 
GTCTTGTCCG AAAACTTTCT GGACTATAAA AACCGTGGAG TCAATGGTTC TCATCGGGGC 
CAGATCATCT GGAAGATCGA TGCCAGCTCG TACTTTGTGG AACCTGAAAC TAAGATCTGC 
TTCAAATACT ACCATGGAGT GAGTGGGGCC CTGCGAGCAA CCACCCCCAG TGTCACGGTC 
AAAAACTCGG CAGCTCCTAT TTTTAAAAGC ATTGGTGCTG ATGAGACCGT CCAAGGACAA 
GGAAGTCGGA GGCTGATCAG CTTCTCTCTC TCAGATTTCC AAGCCATGGG GTTGAAGAAA 
GGGATGTTTT TCAACCCAGA CCCTTATCTG AAGATTTCCA TTCAGCCTGG GAAACACAGC 
ATCTTCCCCG CCCTCCCTCA CCATGGACAG GAGAGGAGAT CCAAGATCAT AGGCAACACC 
GTGAACCCCA TCTGGCAGGC CGAGCAATTC AGTTTTGTGT CCTTGCCCAC TGACGTGCTG 
GAAATTGAGG TGAAGGACAA GTTTGCCAAG AGCCGCCCCA TCATCAAGCG CTTCTTGGGA 
AAGCTGTCGA TGCCCGTTCA AAGACTCCTG GAGAGACACG CCATAGGGGA TAGGGTGGTC 
AGCTACACAC TTGGCCGCAG GCTTCCAACA GATCATGTGA GTGGACAGCT GCAATTCCGA 
TTTGAGATCA CTTCCTCCAT CCACCCAGAT GATGAGGAGA TTTCCCTGAG TACCGAGCCT 
GAGTCAGCCC AAATTCAGGA CAGCCCCATG AACAACCTGA TGGAAAGCGG CAGTGGGGAA 
CCTCGGTCTG AGGCACCAGA GTCCTCTGAG AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC 
AGTGTCCCCG ATGGTCCAGG GAACCAAAGC ATAGAGCTTT CCAGACCAGC TGAGGAAGCA 
GCAGTCATCA CGGAGGCAGG AGACCAGGGC ATGGTCTCTG TGGGACCTGA AGGGGCTGGG 
GAGCTCCTGG CCCAGGTGCA AAAGGACATC CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC 
GAGCAGCTGG ACCTGGGTGA GGAGGCATCA GCACTGCTGC TGGAAGACGG TGAAGCCCCA 
GCCAGCACCA AGGAGGAGCC CTTGGAGGAG GAAGCAACGA CCCAGAGCCG GGCTGGAAGG 
GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG GGAGATGTGT CTACCCTGGA GCAGGGAGAG 
GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCAGCC TGCGGGGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA 
GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC ACCCTCAAGG ACTCCTCGGA GAAGGATGGG 
CTCAGCGAGG TGGACACGGT GGCCGCTGAC CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG 
CCCGAGGGGG CTACTCCAGG CACGGCGCAC CCTGGCCACT CCGGGGGCCA CTTCCCCAGC 
CTGGCCAATG GCGCGGCCCA GGATGGCGAC ACGCACCCCA GCACCGGGAG CGAGAGCGAC 
TCCAGCCCCA GGCAAGGCGG GGACCACAGT TGCGAGGGCT GTGACGCGTC CTGCTGCAGC 
CCCTCGTGCT ACAGCTCCTC GTGCTACAGC ACGTCCTGCT ACAGCAGCTC GTGCTACAGC 
GCCTCGTGCT ACAGCCCCTC CTGCTACAAC GGCAACAGGT TCGCCAGCCA CACGCGCTTC 
TCCTCCGTGG ACAGCGCCAA GATCTCCGAG AGCACGGTCT TCTCCTCGCA AGACGACGAG 
GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA CCCGACTCCA TGCAGAGCCC TGAGCTGGAC 
CCGGAGTCCA CGAACGGCGC TGGGCCGTGG CAAGACGAGC TGGCCGCCCC TAGCGGGCAC 
GTGGAAAGAA GCCCGGAAGG TCTGGAATCC CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA 
GACTGGGAAG CTCGAATTGA CAGCCACGGG CGGGTCTTTT ATGTGGACCA CGTGAACCGC 
ACAACCACCT GGCAGCGTCC GACGGCAGCA GCCACCCCGG ATGGCATGCG GAGATCGGGG 
TCCATCCAGC AGATGGAGCA ACTCAACAGG CGGTATCAAA ACATTCAGCG AACCATTGCA 
ACAGAGAGGT CCGAAGAAGA TTCTGGCAGC CAAAGCTGCG AGCAAGCCCC AGCAGGAGGA 
GGCGGAGGTG GAGGGAGTGA CTCAGAAGCC GAATCTTCCC AGTCCAGCTT AGATCTAAGG 
AGAGAAGGGT CACTTTCTCC AGTGAACTCA CAAAAAATCA CCTTGCTGCT GCAGTCCCCA 
GCGGTCAAGT TCATCACCAA CCCCGAGTTC TTCACTGTGC TACATGCCAA TTATAGTGCC 
TACCGAGTCT TCACCAGTAG CACCTGCTTA AAGCACATGA TTCTGAAAGT CCGACGGGAT 
GCTCGCAATT TTGAACGCTA CCAGCACAAC CGGGACTTGG TGAATTTCAT CAACATGTTC 
GCAGACACTC GGCTGGAACT GCCCCGGGGC TGGGAGATCA AAACGGACCA GCAGGGAAAG 
TCTTTTTTCG TGGACCACAA CAGTCGAGCT ACCACTTTCA TTGACCCCCG AATCCCTCTT 
CAGAACGGTC GTCTTCCCAA TCATCTAACT CACCGACAGC ACCTCCAGAG GCTCCGAAGT 
TACAGCGCTG GAGAGGCCTC AGAAGTTTCT AGAAACAGAG GAGCCTCTTT ACTGGCCAGG 
CCAGGACACA GCTTAGTAGC TGCTATTCGA AGCCAACATC AACATGAGTC ATTGCCACTG 
GCATATAATG ACAAGATTGT GGCATTTCTT CGCCAGCCAA ACATTTTTGA AATGCTGCAA 
GAGCGTCAGC CAAGCTTAGC AAGAAACCAC ACACTCAGGG AGAAAATCCA TTACATTCGG 
ACTGAGGGTA ATCACGGGCT TGAGAAGTTG TCCTGTGATG CGGATCTGGT CATTTTGCTG 
AGTCTCTTTG AAGAAGAGAT TATGTCCTAC GTCCCCCTGC AGGCTGCCTT CCACCCTGGG 
TATAGCTTCT CTCCCCGATG TTCACCCTGT TCTTCACCTC AGAACTCCCC AGGTTTACAG 
AGAGCCAGTG CAAGAGCCCC TTCCCCCTAC CGAAGAGACT TTGAGGCCAA GCTCCGCAAT 
TTCTACAGAA AACTGGAAGC CAAAGGATTT GGTCAGGGTC CGGGGAAAAT TAAGCTCATT 
ATTCGCCGGG ATCATTTGTT GGAGGGAACC TTCAATCAGG TGATGGCCTA TTCGCGGAAA 
GAGCTCCAGC GAAACAAGCT CTACGTCACC TTTGTTGGAG AGGAGGGCCT GGACTACAGT 
GGCCCCTCGC GGGAGTTCTT CTTCCTTCTG TCTCAGGAGC TCTTCAACCC TTACTATGGA 
CTCTTTGAGT ACTCGGCAAA TGATACTTAC ACGGTGCAGA TCAGCCCCAT GTCCGCATTT 
GTAGAAAACC ATCTTGAGTG GTTCAGGTTT AGCGGTCGCA TCCTGGGTCT GGCTCTGATC 
CATCAGTACC TTCTTGACGC TTTCTTCACG AGGCCCTTCT ACAAGGCACT CCTGAGACTG 
CCCTGTGATT TGAGTGACCT GGAATATTTG GATGAGGAAT TCCACCAGAG TTTGCAGTGG 
ATGAAGGACA ACAACATCAC AGACATCTTA GACCTCACTT TCACTGTTAA TGAAGAGGTT 
TTTGGACAGG TCACGGAAAG GGAGTTGAAG TCTGGAGGAG CCAACACACA GGTGACGGAG 
AAAAACAAGA AGGAGTACAT CGAGCGCATG GTGAAGTGGC GGGTGGAGCG CGGCGTGGTA 
CAGCAGACCG AGGCGCTGGT GCGCGGCTTC TACGAGGTTG TAGACTCGAG GCTGGTGTCC 
GTGTTTGATG CCAGGGAGCT GGAGCTGGTG ATAGCTGGCA CCGCGGAAAT CGACCTAAAT 
GACTGGCGGA ATAACACTGA GTACCGGGGA GGTTACCACG ATGGGCATCT TGTGATCCGC 
TGGTTCTGGG CTGCGGTGGA GCGCTTCAAT AATGAGCAGA GGCTGAGATT ACTGCAGTTT 
GTCACGGGAA CATCCAGCGT GCCCTACGAA GGCTTCGCAG CCCTCCGTGG GAGCAATGGG 
CTTCGGCGCT TCTGCATAGA GAAATGGGGG AAAATTACTT CTCTCCCCAG GGCACACACA 
TGCTTCAACC GACTGGATCT TCCACCGTAT CCCTCGTACT CCATGTTGTA TGAAAAGCTG 
TTAACAGCAG TAGAGGAAAC CAGCACCTTT GGACTTGAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_011515224.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513526.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu65383
Clone ID Related Accession (Same CDS sequence)	XM_011515224.2 , XM_011515222.2 , XM_011515223.2
Accession Version	XM_011515224.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515224.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTC CTTCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAGGAGCC GCTCCGATAC 
AGCTACAACC CCGACCAGTT CCACAACATG GACCTCAGGG GCGGCCCCCA CGATGGCGTC 
ACCATTCCCC GCTCCACCAG CGACACTGAC CTGGTCACCT CGGACAGCCG CTCCACGCTC 
ATGGTCAGCA GCTCCTACTA TTCCATCGGG CACTCTCAGG ACCTGGTCAT CCACTGGGAC 
ATAAAGGAGG AAGTGGACGC TGGGGACTGG ATTGGCATGT ACCTCATTGA TGAGGTCTTG 
TCCGAAAACT TTCTGGACTA TAAAAACCGT GGAGTCAATG GTTCTCATCG GGGCCAGATC 
ATCTGGAAGA TCGATGCCAG CTCGTACTTT GTGGAACCTG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG GGCCCTGCGA GCAACCACCC CCAGTGTCAC GGTCAAAAAC 
TCGGCAGCTC CTATTTTTAA AAGCATTGGT GCTGATGAGA CCGTCCAAGG ACAAGGAAGT 
CGGAGGCTGA TCAGCTTCTC TCTCTCAGAT TTCCAAGCCA TGGGGTTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA TCTGAAGATT TCCATTCAGC CTGGGAAACA CAGCATCTTC 
CCCGCCCTCC CTCACCATGG ACAGGAGAGG AGATCCAAGA TCATAGGCAA CACCGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGTTTT GTGTCCTTGC CCACTGACGT GCTGGAAATT 
GAGGTGAAGG ACAAGTTTGC CAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAAAGACT CCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCCT CCATCCACCC AGCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT ACATGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGATGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAGTTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGTCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACGGAGA AAAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACTGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513526.1
CDS	661..5421
Translation	MASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_011515224.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515224.2

ORF Insert Sequence:

ATGGCGTCTC CTTCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAGGAGCC GCTCCGATAC 
AGCTACAACC CCGACCAGTT CCACAACATG GACCTCAGGG GCGGCCCCCA CGATGGCGTC 
ACCATTCCCC GCTCCACCAG CGACACTGAC CTGGTCACCT CGGACAGCCG CTCCACGCTC 
ATGGTCAGCA GCTCCTACTA TTCCATCGGG CACTCTCAGG ACCTGGTCAT CCACTGGGAC 
ATAAAGGAGG AAGTGGACGC TGGGGACTGG ATTGGCATGT ACCTCATTGA TGAGGTCTTG 
TCCGAAAACT TTCTGGACTA TAAAAACCGT GGAGTCAATG GTTCTCATCG GGGCCAGATC 
ATCTGGAAGA TCGATGCCAG CTCGTACTTT GTGGAACCTG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG GGCCCTGCGA GCAACCACCC CCAGTGTCAC GGTCAAAAAC 
TCGGCAGCTC CTATTTTTAA AAGCATTGGT GCTGATGAGA CCGTCCAAGG ACAAGGAAGT 
CGGAGGCTGA TCAGCTTCTC TCTCTCAGAT TTCCAAGCCA TGGGGTTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA TCTGAAGATT TCCATTCAGC CTGGGAAACA CAGCATCTTC 
CCCGCCCTCC CTCACCATGG ACAGGAGAGG AGATCCAAGA TCATAGGCAA CACCGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGTTTT GTGTCCTTGC CCACTGACGT GCTGGAAATT 
GAGGTGAAGG ACAAGTTTGC CAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAAAGACT CCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCCT CCATCCACCC AGCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT ACATGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGATGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAGTTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGTCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACGGAGA AAAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACTGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_011515222.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513524.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X12, mRNA.

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CloneID	OHu65383
Clone ID Related Accession (Same CDS sequence)	XM_011515224.2 , XM_011515222.2 , XM_011515223.2
Accession Version	XM_011515222.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515222.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTC CTTCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAGGAGCC GCTCCGATAC 
AGCTACAACC CCGACCAGTT CCACAACATG GACCTCAGGG GCGGCCCCCA CGATGGCGTC 
ACCATTCCCC GCTCCACCAG CGACACTGAC CTGGTCACCT CGGACAGCCG CTCCACGCTC 
ATGGTCAGCA GCTCCTACTA TTCCATCGGG CACTCTCAGG ACCTGGTCAT CCACTGGGAC 
ATAAAGGAGG AAGTGGACGC TGGGGACTGG ATTGGCATGT ACCTCATTGA TGAGGTCTTG 
TCCGAAAACT TTCTGGACTA TAAAAACCGT GGAGTCAATG GTTCTCATCG GGGCCAGATC 
ATCTGGAAGA TCGATGCCAG CTCGTACTTT GTGGAACCTG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG GGCCCTGCGA GCAACCACCC CCAGTGTCAC GGTCAAAAAC 
TCGGCAGCTC CTATTTTTAA AAGCATTGGT GCTGATGAGA CCGTCCAAGG ACAAGGAAGT 
CGGAGGCTGA TCAGCTTCTC TCTCTCAGAT TTCCAAGCCA TGGGGTTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA TCTGAAGATT TCCATTCAGC CTGGGAAACA CAGCATCTTC 
CCCGCCCTCC CTCACCATGG ACAGGAGAGG AGATCCAAGA TCATAGGCAA CACCGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGTTTT GTGTCCTTGC CCACTGACGT GCTGGAAATT 
GAGGTGAAGG ACAAGTTTGC CAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAAAGACT CCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCCT CCATCCACCC AGCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT ACATGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGATGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAGTTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGTCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACGGAGA AAAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACTGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513524.1
CDS	380..5140
Translation	MASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_011515222.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515222.2

ORF Insert Sequence:

ATGGCGTCTC CTTCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAGGAGCC GCTCCGATAC 
AGCTACAACC CCGACCAGTT CCACAACATG GACCTCAGGG GCGGCCCCCA CGATGGCGTC 
ACCATTCCCC GCTCCACCAG CGACACTGAC CTGGTCACCT CGGACAGCCG CTCCACGCTC 
ATGGTCAGCA GCTCCTACTA TTCCATCGGG CACTCTCAGG ACCTGGTCAT CCACTGGGAC 
ATAAAGGAGG AAGTGGACGC TGGGGACTGG ATTGGCATGT ACCTCATTGA TGAGGTCTTG 
TCCGAAAACT TTCTGGACTA TAAAAACCGT GGAGTCAATG GTTCTCATCG GGGCCAGATC 
ATCTGGAAGA TCGATGCCAG CTCGTACTTT GTGGAACCTG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG GGCCCTGCGA GCAACCACCC CCAGTGTCAC GGTCAAAAAC 
TCGGCAGCTC CTATTTTTAA AAGCATTGGT GCTGATGAGA CCGTCCAAGG ACAAGGAAGT 
CGGAGGCTGA TCAGCTTCTC TCTCTCAGAT TTCCAAGCCA TGGGGTTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA TCTGAAGATT TCCATTCAGC CTGGGAAACA CAGCATCTTC 
CCCGCCCTCC CTCACCATGG ACAGGAGAGG AGATCCAAGA TCATAGGCAA CACCGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGTTTT GTGTCCTTGC CCACTGACGT GCTGGAAATT 
GAGGTGAAGG ACAAGTTTGC CAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAAAGACT CCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCCT CCATCCACCC AGCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT ACATGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGATGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAGTTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGTCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACGGAGA AAAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACTGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_011515223.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513525.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X13, mRNA.

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CloneID	OHu65383
Clone ID Related Accession (Same CDS sequence)	XM_011515224.2 , XM_011515222.2 , XM_011515223.2
Accession Version	XM_011515223.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515223.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTC CTTCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAGGAGCC GCTCCGATAC 
AGCTACAACC CCGACCAGTT CCACAACATG GACCTCAGGG GCGGCCCCCA CGATGGCGTC 
ACCATTCCCC GCTCCACCAG CGACACTGAC CTGGTCACCT CGGACAGCCG CTCCACGCTC 
ATGGTCAGCA GCTCCTACTA TTCCATCGGG CACTCTCAGG ACCTGGTCAT CCACTGGGAC 
ATAAAGGAGG AAGTGGACGC TGGGGACTGG ATTGGCATGT ACCTCATTGA TGAGGTCTTG 
TCCGAAAACT TTCTGGACTA TAAAAACCGT GGAGTCAATG GTTCTCATCG GGGCCAGATC 
ATCTGGAAGA TCGATGCCAG CTCGTACTTT GTGGAACCTG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG GGCCCTGCGA GCAACCACCC CCAGTGTCAC GGTCAAAAAC 
TCGGCAGCTC CTATTTTTAA AAGCATTGGT GCTGATGAGA CCGTCCAAGG ACAAGGAAGT 
CGGAGGCTGA TCAGCTTCTC TCTCTCAGAT TTCCAAGCCA TGGGGTTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA TCTGAAGATT TCCATTCAGC CTGGGAAACA CAGCATCTTC 
CCCGCCCTCC CTCACCATGG ACAGGAGAGG AGATCCAAGA TCATAGGCAA CACCGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGTTTT GTGTCCTTGC CCACTGACGT GCTGGAAATT 
GAGGTGAAGG ACAAGTTTGC CAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAAAGACT CCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCCT CCATCCACCC AGCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT ACATGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGATGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAGTTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGTCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACGGAGA AAAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACTGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513525.1
CDS	181..4941
Translation	MASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_011515223.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515223.2

ORF Insert Sequence:

ATGGCGTCTC CTTCTAGAAA CTCCCAGAGC CGACGCCGGT GCAAGGAGCC GCTCCGATAC 
AGCTACAACC CCGACCAGTT CCACAACATG GACCTCAGGG GCGGCCCCCA CGATGGCGTC 
ACCATTCCCC GCTCCACCAG CGACACTGAC CTGGTCACCT CGGACAGCCG CTCCACGCTC 
ATGGTCAGCA GCTCCTACTA TTCCATCGGG CACTCTCAGG ACCTGGTCAT CCACTGGGAC 
ATAAAGGAGG AAGTGGACGC TGGGGACTGG ATTGGCATGT ACCTCATTGA TGAGGTCTTG 
TCCGAAAACT TTCTGGACTA TAAAAACCGT GGAGTCAATG GTTCTCATCG GGGCCAGATC 
ATCTGGAAGA TCGATGCCAG CTCGTACTTT GTGGAACCTG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GAGTGAGTGG GGCCCTGCGA GCAACCACCC CCAGTGTCAC GGTCAAAAAC 
TCGGCAGCTC CTATTTTTAA AAGCATTGGT GCTGATGAGA CCGTCCAAGG ACAAGGAAGT 
CGGAGGCTGA TCAGCTTCTC TCTCTCAGAT TTCCAAGCCA TGGGGTTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA TCTGAAGATT TCCATTCAGC CTGGGAAACA CAGCATCTTC 
CCCGCCCTCC CTCACCATGG ACAGGAGAGG AGATCCAAGA TCATAGGCAA CACCGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGTTTT GTGTCCTTGC CCACTGACGT GCTGGAAATT 
GAGGTGAAGG ACAAGTTTGC CAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAAAGACT CCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAC 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCCT CCATCCACCC AGCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT ACATGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGATGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAGTTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGG 
TTCAGGTTTA GCGGTCGCAT CCTGGGTCTG GCTCTGATCC ATCAGTACCT TCTTGACGCT 
TTCTTCACGA GGCCCTTCTA CAAGGCACTC CTGAGACTGC CCTGTGATTT GAGTGACCTG 
GAATATTTGG ATGAGGAATT CCACCAGAGT TTGCAGTGGA TGAAGGACAA CAACATCACA 
GACATCTTAG ACCTCACTTT CACTGTTAAT GAAGAGGTTT TTGGACAGGT CACGGAAAGG 
GAGTTGAAGT CTGGAGGAGC CAACACACAG GTGACGGAGA AAAACAAGAA GGAGTACATC 
GAGCGCATGG TGAAGTGGCG GGTGGAGCGC GGCGTGGTAC AGCAGACCGA GGCGCTGGTG 
CGCGGCTTCT ACGAGGTTGT AGACTCGAGG CTGGTGTCCG TGTTTGATGC CAGGGAGCTG 
GAGCTGGTGA TAGCTGGCAC CGCGGAAATC GACCTAAATG ACTGGCGGAA TAACACTGAG 
TACCGGGGAG GTTACCACGA TGGGCATCTT GTGATCCGCT GGTTCTGGGC TGCGGTGGAG 
CGCTTCAATA ATGAGCAGAG GCTGAGATTA CTGCAGTTTG TCACGGGAAC ATCCAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTCCGTGGG AGCAATGGGC TTCGGCGCTT CTGCATAGAG 
AAATGGGGGA AAATTACTTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCGTATC CCTCGTACTC CATGTTGTAT GAAAAGCTGT TAACAGCAGT AGAGGAAACC 
AGCACCTTTG GACTTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_011515226.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513528.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X18, mRNA.

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CloneID	OHu65385
Clone ID Related Accession (Same CDS sequence)	XM_011515226.2 , XM_011515226.3
Accession Version	XM_011515226.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515226.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC TGGCAGAACA CAAAGATGAC TGCATTTCCA AGGAGATTTT TAAAAGCATT 
GGTGCTGATG AGACCGTCCA AGGACAAGGA AGTCGGAGGC TGATCAGCTT CTCTCTCTCA 
GATTTCCAAG CCATGGGGTT GAAGAAAGGG ATGTTTTTCA ACCCAGACCC TTATCTGAAG 
ATTTCCATTC AGCCTGGGAA ACACAGCATC TTCCCCGCCC TCCCTCACCA TGGACAGGAG 
AGGAGATCCA AGATCATAGG CAACACCGTG AACCCCATCT GGCAGGCCGA GCAATTCAGT 
TTTGTGTCCT TGCCCACTGA CGTGCTGGAA ATTGAGGTGA AGGACAAGTT TGCCAAGAGC 
CGCCCCATCA TCAAGCGCTT CTTGGGAAAG CTGTCGATGC CCGTTCAAAG ACTCCTGGAG 
AGACACGCCA TAGGGGATAG GGTGGTCAGC TACACACTTG GCCGCAGGCT TCCAACAGAT 
CATGTGAGTG GACAGCTGCA ATTCCGATTT GAGATCACTT CCTCCATCCA CCCAGCAGAT 
GATGAGGAGA TTTCCCTGAG TACCGAGCCT GAGTCAGCCC AAATTCAGGA CAGCCCCATG 
AACAACCTGA TGGAAAGCGG CAGTGGGGAA CCTCGGTCTG AGGCACCAGA GTCCTCTGAG 
AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC AGTGTCCCCG ATGGTCCAGG GAACCAAAGC 
ATAGAGCTTT CCAGACCAGC TGAGGAAGCA GCAGTCATCA CGGAGGCAGG AGACCAGGGC 
ATGGTCTCTG TGGGACCTGA AGGGGCTGGG GAGCTCCTGG CCCAGGTGCA AAAGGACATC 
CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC GAGCAGCTGG ACCTGGGTGA GGAGGCATCA 
GCACTGCTGC TGGAAGACGG TGAAGCCCCA GCCAGCACCA AGGAGGAGCC CTTGGAGGAG 
GAAGCAACGA CCCAGAGCCG GGCTGGAAGG GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG 
GGAGATGTGT CTACCCTGGA GCAGGGAGAG GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG 
AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG TCCGAGCTGG AGACGGTGAT CGCGTCAGCC 
TGCGGGGACC CCGAGACCCC GCGGACACAC TACATCCGCA TCCACACCCT GCTGCACAGC 
ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC 
ACCCTCAAGG ACTCCTCGGA GAAGGATGGG CTCAGCGAGG TGGACACGGT GGCCGCTGAC 
CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG CCCGAGGGGG CTACTCCAGG CACGGCGCAC 
CCTGGCCACT CCGGGGGCCA CTTCCCCAGC CTGGCCAATG GCGCGGCCCA GGATGGCGAC 
ACGCACCCCA GCACCGGGAG CGAGAGCGAC TCCAGCCCCA GGCAAGGCGG GGACCACAGT 
TGCGAGGGCT GTGACGCGTC CTGCTGCAGC CCCTCGTGCT ACAGCTCCTC GTGCTACAGC 
ACGTCCTGCT ACAGCAGCTC GTGCTACAGC GCCTCGTGCT ACAGCCCCTC CTGCTACAAC 
GGCAACAGGT TCGCCAGCCA CACGCGCTTC TCCTCCGTGG ACAGCGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA AGACGACGAG GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA 
CCCGACTCCA TGCAGAGCCC TGAGCTGGAC CCGGAGTCCA CGAACGGCGC TGGGCCGTGG 
CAAGACGAGC TGGCCGCCCC TAGCGGGCAC GTGGAAAGAA GCCCGGAAGG TCTGGAATCC 
CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAACTGGGA AGCTCGAATT GACAGCCACG GGCGGGTCTT TTATGTGGAC 
CACGTGAACC GCACAACCAC CTGGCAGCGT CCGACGGCAG CAGCCACCCC GGATGGCATG 
CGGAGATCGG GGTCCATCCA GCAGATGGAG CAACTCAACA GGCGGTATCA AAACATTCAG 
CGAACCATTG CAACAGAGAG GTCCGAAGAA GATTCTGGCA GCCAAAGCTG CGAGCAAGCC 
CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT GACTCAGAAG CCGAATCTTC CCAGTCCAGC 
TTAGATCTAA GGAGAGAAGG GTCACTTTCT CCAGTGAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCCC CAGCGGTCAA GTTCATCACC AACCCCGAGT TCTTCACTGT GCTACATGCC 
AATTATAGTG CCTACCGAGT CTTCACCAGT AGCACCTGCT TAAAGCACAT GATTCTGAAA 
GTCCGACGGG ATGCTCGCAA TTTTGAACGC TACCAGCACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TCGCAGACAC TCGGCTGGAA CTGCCCCGGG GCTGGGAGAT CAAAACGGAC 
CAGCAGGGAA AGTCTTTTTT CGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAACGG TCGTCTTCCC AATCATCTAA CTCACCGACA GCACCTCCAG 
AGGCTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTTT CTAGAAACAG AGGAGCCTCT 
TTACTGGCCA GGCCAGGACA CAGCTTAGTA GCTGCTATTC GAAGCCAACA TCAACATGAG 
TCATTGCCAC TGGCATATAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTGC AAGAGCGTCA GCCAAGCTTA GCAAGAAACC ACACACTCAG GGAGAAAATC 
CATTACATTC GGACTGAGGG TAATCACGGG CTTGAGAAGT TGTCCTGTGA TGCGGATCTG 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATTATGTCCT ACGTCCCCCT GCAGGCTGCC 
TTCCACCCTG GGTATAGCTT CTCTCCCCGA TGTTCACCCT GTTCTTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTACAG AAAACTGGAA GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA 
ATTAAGCTCA TTATTCGCCG GGATCATTTG TTGGAGGGAA CCTTCAATCA GGTGATGGCC 
TATTCGCGGA AAGAGCTCCA GCGAAACAAG CTCTACGTCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGCCCCTC GCGGGAGTTC TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC 
CCTTACTATG GACTCTTTGA GTACTCGGCA AATGATACTT ACACGGTGCA GATCAGCCCC 
ATGTCCGCAT TTGTAGAAAA CCATCTTGAG TGGTTCAGGT TTAGCGGTCG CATCCTGGGT 
CTGGCTCTGA TCCATCAGTA CCTTCTTGAC GCTTTCTTCA CGAGGCCCTT CTACAAGGCA 
CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC CTGGAATATT TGGATGAGGA ATTCCACCAG 
AGTTTGCAGT GGATGAAGGA CAACAACATC ACAGACATCT TAGACCTCAC TTTCACTGTT 
AATGAAGAGG TTTTTGGACA GGTCACGGAA AGGGAGTTGA AGTCTGGAGG AGCCAACACA 
CAGGTGACGG AGAAAAACAA GAAGGAGTAC ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG 
CGCGGCGTGG TACAGCAGAC CGAGGCGCTG GTGCGCGGCT TCTACGAGGT TGTAGACTCG 
AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG CTGGAGCTGG TGATAGCTGG CACCGCGGAA 
ATCGACCTAA ATGACTGGCG GAATAACACT GAGTACCGGG GAGGTTACCA CGATGGGCAT 
CTTGTGATCC GCTGGTTCTG GGCTGCGGTG GAGCGCTTCA ATAATGAGCA GAGGCTGAGA 
TTACTGCAGT TTGTCACGGG AACATCCAGC GTGCCCTACG AAGGCTTCGC AGCCCTCCGT 
GGGAGCAATG GGCTTCGGCG CTTCTGCATA GAGAAATGGG GGAAAATTAC TTCTCTCCCC 
AGGGCACACA CATGCTTCAA CCGACTGGAT CTTCCACCGT ATCCCTCGTA CTCCATGTTG 
TATGAAAAGC TGTTAACAGC AGTAGAGGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513528.1
CDS	369..4682
Translation	MAALAEHKDDCISKEIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_011515226.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515226.2

ORF Insert Sequence:

ATGGCTGCTC TGGCAGAACA CAAAGATGAC TGCATTTCCA AGGAGATTTT TAAAAGCATT 
GGTGCTGATG AGACCGTCCA AGGACAAGGA AGTCGGAGGC TGATCAGCTT CTCTCTCTCA 
GATTTCCAAG CCATGGGGTT GAAGAAAGGG ATGTTTTTCA ACCCAGACCC TTATCTGAAG 
ATTTCCATTC AGCCTGGGAA ACACAGCATC TTCCCCGCCC TCCCTCACCA TGGACAGGAG 
AGGAGATCCA AGATCATAGG CAACACCGTG AACCCCATCT GGCAGGCCGA GCAATTCAGT 
TTTGTGTCCT TGCCCACTGA CGTGCTGGAA ATTGAGGTGA AGGACAAGTT TGCCAAGAGC 
CGCCCCATCA TCAAGCGCTT CTTGGGAAAG CTGTCGATGC CCGTTCAAAG ACTCCTGGAG 
AGACACGCCA TAGGGGATAG GGTGGTCAGC TACACACTTG GCCGCAGGCT TCCAACAGAT 
CATGTGAGTG GACAGCTGCA ATTCCGATTT GAGATCACTT CCTCCATCCA CCCAGCAGAT 
GATGAGGAGA TTTCCCTGAG TACCGAGCCT GAGTCAGCCC AAATTCAGGA CAGCCCCATG 
AACAACCTGA TGGAAAGCGG CAGTGGGGAA CCTCGGTCTG AGGCACCAGA GTCCTCTGAG 
AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC AGTGTCCCCG ATGGTCCAGG GAACCAAAGC 
ATAGAGCTTT CCAGACCAGC TGAGGAAGCA GCAGTCATCA CGGAGGCAGG AGACCAGGGC 
ATGGTCTCTG TGGGACCTGA AGGGGCTGGG GAGCTCCTGG CCCAGGTGCA AAAGGACATC 
CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC GAGCAGCTGG ACCTGGGTGA GGAGGCATCA 
GCACTGCTGC TGGAAGACGG TGAAGCCCCA GCCAGCACCA AGGAGGAGCC CTTGGAGGAG 
GAAGCAACGA CCCAGAGCCG GGCTGGAAGG GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG 
GGAGATGTGT CTACCCTGGA GCAGGGAGAG GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG 
AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG TCCGAGCTGG AGACGGTGAT CGCGTCAGCC 
TGCGGGGACC CCGAGACCCC GCGGACACAC TACATCCGCA TCCACACCCT GCTGCACAGC 
ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC 
ACCCTCAAGG ACTCCTCGGA GAAGGATGGG CTCAGCGAGG TGGACACGGT GGCCGCTGAC 
CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG CCCGAGGGGG CTACTCCAGG CACGGCGCAC 
CCTGGCCACT CCGGGGGCCA CTTCCCCAGC CTGGCCAATG GCGCGGCCCA GGATGGCGAC 
ACGCACCCCA GCACCGGGAG CGAGAGCGAC TCCAGCCCCA GGCAAGGCGG GGACCACAGT 
TGCGAGGGCT GTGACGCGTC CTGCTGCAGC CCCTCGTGCT ACAGCTCCTC GTGCTACAGC 
ACGTCCTGCT ACAGCAGCTC GTGCTACAGC GCCTCGTGCT ACAGCCCCTC CTGCTACAAC 
GGCAACAGGT TCGCCAGCCA CACGCGCTTC TCCTCCGTGG ACAGCGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA AGACGACGAG GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA 
CCCGACTCCA TGCAGAGCCC TGAGCTGGAC CCGGAGTCCA CGAACGGCGC TGGGCCGTGG 
CAAGACGAGC TGGCCGCCCC TAGCGGGCAC GTGGAAAGAA GCCCGGAAGG TCTGGAATCC 
CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAACTGGGA AGCTCGAATT GACAGCCACG GGCGGGTCTT TTATGTGGAC 
CACGTGAACC GCACAACCAC CTGGCAGCGT CCGACGGCAG CAGCCACCCC GGATGGCATG 
CGGAGATCGG GGTCCATCCA GCAGATGGAG CAACTCAACA GGCGGTATCA AAACATTCAG 
CGAACCATTG CAACAGAGAG GTCCGAAGAA GATTCTGGCA GCCAAAGCTG CGAGCAAGCC 
CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT GACTCAGAAG CCGAATCTTC CCAGTCCAGC 
TTAGATCTAA GGAGAGAAGG GTCACTTTCT CCAGTGAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCCC CAGCGGTCAA GTTCATCACC AACCCCGAGT TCTTCACTGT GCTACATGCC 
AATTATAGTG CCTACCGAGT CTTCACCAGT AGCACCTGCT TAAAGCACAT GATTCTGAAA 
GTCCGACGGG ATGCTCGCAA TTTTGAACGC TACCAGCACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TCGCAGACAC TCGGCTGGAA CTGCCCCGGG GCTGGGAGAT CAAAACGGAC 
CAGCAGGGAA AGTCTTTTTT CGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAACGG TCGTCTTCCC AATCATCTAA CTCACCGACA GCACCTCCAG 
AGGCTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTTT CTAGAAACAG AGGAGCCTCT 
TTACTGGCCA GGCCAGGACA CAGCTTAGTA GCTGCTATTC GAAGCCAACA TCAACATGAG 
TCATTGCCAC TGGCATATAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTGC AAGAGCGTCA GCCAAGCTTA GCAAGAAACC ACACACTCAG GGAGAAAATC 
CATTACATTC GGACTGAGGG TAATCACGGG CTTGAGAAGT TGTCCTGTGA TGCGGATCTG 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATTATGTCCT ACGTCCCCCT GCAGGCTGCC 
TTCCACCCTG GGTATAGCTT CTCTCCCCGA TGTTCACCCT GTTCTTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTACAG AAAACTGGAA GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA 
ATTAAGCTCA TTATTCGCCG GGATCATTTG TTGGAGGGAA CCTTCAATCA GGTGATGGCC 
TATTCGCGGA AAGAGCTCCA GCGAAACAAG CTCTACGTCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGCCCCTC GCGGGAGTTC TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC 
CCTTACTATG GACTCTTTGA GTACTCGGCA AATGATACTT ACACGGTGCA GATCAGCCCC 
ATGTCCGCAT TTGTAGAAAA CCATCTTGAG TGGTTCAGGT TTAGCGGTCG CATCCTGGGT 
CTGGCTCTGA TCCATCAGTA CCTTCTTGAC GCTTTCTTCA CGAGGCCCTT CTACAAGGCA 
CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC CTGGAATATT TGGATGAGGA ATTCCACCAG 
AGTTTGCAGT GGATGAAGGA CAACAACATC ACAGACATCT TAGACCTCAC TTTCACTGTT 
AATGAAGAGG TTTTTGGACA GGTCACGGAA AGGGAGTTGA AGTCTGGAGG AGCCAACACA 
CAGGTGACGG AGAAAAACAA GAAGGAGTAC ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG 
CGCGGCGTGG TACAGCAGAC CGAGGCGCTG GTGCGCGGCT TCTACGAGGT TGTAGACTCG 
AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG CTGGAGCTGG TGATAGCTGG CACCGCGGAA 
ATCGACCTAA ATGACTGGCG GAATAACACT GAGTACCGGG GAGGTTACCA CGATGGGCAT 
CTTGTGATCC GCTGGTTCTG GGCTGCGGTG GAGCGCTTCA ATAATGAGCA GAGGCTGAGA 
TTACTGCAGT TTGTCACGGG AACATCCAGC GTGCCCTACG AAGGCTTCGC AGCCCTCCGT 
GGGAGCAATG GGCTTCGGCG CTTCTGCATA GAGAAATGGG GGAAAATTAC TTCTCTCCCC 
AGGGCACACA CATGCTTCAA CCGACTGGAT CTTCCACCGT ATCCCTCGTA CTCCATGTTG 
TATGAAAAGC TGTTAACAGC AGTAGAGGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_011515226.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513528.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X18, mRNA.

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CloneID	OHu65385
Clone ID Related Accession (Same CDS sequence)	XM_011515226.2 , XM_011515226.3
Accession Version	XM_011515226.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011515226.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTC TGGCAGAACA CAAAGATGAC TGCATTTCCA AGGAGATTTT TAAAAGCATT 
GGTGCTGATG AGACCGTCCA AGGACAAGGA AGTCGGAGGC TGATCAGCTT CTCTCTCTCA 
GATTTCCAAG CCATGGGGTT GAAGAAAGGG ATGTTTTTCA ACCCAGACCC TTATCTGAAG 
ATTTCCATTC AGCCTGGGAA ACACAGCATC TTCCCCGCCC TCCCTCACCA TGGACAGGAG 
AGGAGATCCA AGATCATAGG CAACACCGTG AACCCCATCT GGCAGGCCGA GCAATTCAGT 
TTTGTGTCCT TGCCCACTGA CGTGCTGGAA ATTGAGGTGA AGGACAAGTT TGCCAAGAGC 
CGCCCCATCA TCAAGCGCTT CTTGGGAAAG CTGTCGATGC CCGTTCAAAG ACTCCTGGAG 
AGACACGCCA TAGGGGATAG GGTGGTCAGC TACACACTTG GCCGCAGGCT TCCAACAGAT 
CATGTGAGTG GACAGCTGCA ATTCCGATTT GAGATCACTT CCTCCATCCA CCCAGCAGAT 
GATGAGGAGA TTTCCCTGAG TACCGAGCCT GAGTCAGCCC AAATTCAGGA CAGCCCCATG 
AACAACCTGA TGGAAAGCGG CAGTGGGGAA CCTCGGTCTG AGGCACCAGA GTCCTCTGAG 
AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC AGTGTCCCCG ATGGTCCAGG GAACCAAAGC 
ATAGAGCTTT CCAGACCAGC TGAGGAAGCA GCAGTCATCA CGGAGGCAGG AGACCAGGGC 
ATGGTCTCTG TGGGACCTGA AGGGGCTGGG GAGCTCCTGG CCCAGGTGCA AAAGGACATC 
CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC GAGCAGCTGG ACCTGGGTGA GGAGGCATCA 
GCACTGCTGC TGGAAGACGG TGAAGCCCCA GCCAGCACCA AGGAGGAGCC CTTGGAGGAG 
GAAGCAACGA CCCAGAGCCG GGCTGGAAGG GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG 
GGAGATGTGT CTACCCTGGA GCAGGGAGAG GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG 
AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG TCCGAGCTGG AGACGGTGAT CGCGTCAGCC 
TGCGGGGACC CCGAGACCCC GCGGACACAC TACATCCGCA TCCACACCCT GCTGCACAGC 
ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC 
ACCCTCAAGG ACTCCTCGGA GAAGGATGGG CTCAGCGAGG TGGACACGGT GGCCGCTGAC 
CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG CCCGAGGGGG CTACTCCAGG CACGGCGCAC 
CCTGGCCACT CCGGGGGCCA CTTCCCCAGC CTGGCCAATG GCGCGGCCCA GGATGGCGAC 
ACGCACCCCA GCACCGGGAG CGAGAGCGAC TCCAGCCCCA GGCAAGGCGG GGACCACAGT 
TGCGAGGGCT GTGACGCGTC CTGCTGCAGC CCCTCGTGCT ACAGCTCCTC GTGCTACAGC 
ACGTCCTGCT ACAGCAGCTC GTGCTACAGC GCCTCGTGCT ACAGCCCCTC CTGCTACAAC 
GGCAACAGGT TCGCCAGCCA CACGCGCTTC TCCTCCGTGG ACAGCGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA AGACGACGAG GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA 
CCCGACTCCA TGCAGAGCCC TGAGCTGGAC CCGGAGTCCA CGAACGGCGC TGGGCCGTGG 
CAAGACGAGC TGGCCGCCCC TAGCGGGCAC GTGGAAAGAA GCCCGGAAGG TCTGGAATCC 
CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAACTGGGA AGCTCGAATT GACAGCCACG GGCGGGTCTT TTATGTGGAC 
CACGTGAACC GCACAACCAC CTGGCAGCGT CCGACGGCAG CAGCCACCCC GGATGGCATG 
CGGAGATCGG GGTCCATCCA GCAGATGGAG CAACTCAACA GGCGGTATCA AAACATTCAG 
CGAACCATTG CAACAGAGAG GTCCGAAGAA GATTCTGGCA GCCAAAGCTG CGAGCAAGCC 
CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT GACTCAGAAG CCGAATCTTC CCAGTCCAGC 
TTAGATCTAA GGAGAGAAGG GTCACTTTCT CCAGTGAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCCC CAGCGGTCAA GTTCATCACC AACCCCGAGT TCTTCACTGT GCTACATGCC 
AATTATAGTG CCTACCGAGT CTTCACCAGT AGCACCTGCT TAAAGCACAT GATTCTGAAA 
GTCCGACGGG ATGCTCGCAA TTTTGAACGC TACCAGCACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TCGCAGACAC TCGGCTGGAA CTGCCCCGGG GCTGGGAGAT CAAAACGGAC 
CAGCAGGGAA AGTCTTTTTT CGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAACGG TCGTCTTCCC AATCATCTAA CTCACCGACA GCACCTCCAG 
AGGCTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTTT CTAGAAACAG AGGAGCCTCT 
TTACTGGCCA GGCCAGGACA CAGCTTAGTA GCTGCTATTC GAAGCCAACA TCAACATGAG 
TCATTGCCAC TGGCATATAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTGC AAGAGCGTCA GCCAAGCTTA GCAAGAAACC ACACACTCAG GGAGAAAATC 
CATTACATTC GGACTGAGGG TAATCACGGG CTTGAGAAGT TGTCCTGTGA TGCGGATCTG 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATTATGTCCT ACGTCCCCCT GCAGGCTGCC 
TTCCACCCTG GGTATAGCTT CTCTCCCCGA TGTTCACCCT GTTCTTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTACAG AAAACTGGAA GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA 
ATTAAGCTCA TTATTCGCCG GGATCATTTG TTGGAGGGAA CCTTCAATCA GGTGATGGCC 
TATTCGCGGA AAGAGCTCCA GCGAAACAAG CTCTACGTCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGCCCCTC GCGGGAGTTC TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC 
CCTTACTATG GACTCTTTGA GTACTCGGCA AATGATACTT ACACGGTGCA GATCAGCCCC 
ATGTCCGCAT TTGTAGAAAA CCATCTTGAG TGGTTCAGGT TTAGCGGTCG CATCCTGGGT 
CTGGCTCTGA TCCATCAGTA CCTTCTTGAC GCTTTCTTCA CGAGGCCCTT CTACAAGGCA 
CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC CTGGAATATT TGGATGAGGA ATTCCACCAG 
AGTTTGCAGT GGATGAAGGA CAACAACATC ACAGACATCT TAGACCTCAC TTTCACTGTT 
AATGAAGAGG TTTTTGGACA GGTCACGGAA AGGGAGTTGA AGTCTGGAGG AGCCAACACA 
CAGGTGACGG AGAAAAACAA GAAGGAGTAC ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG 
CGCGGCGTGG TACAGCAGAC CGAGGCGCTG GTGCGCGGCT TCTACGAGGT TGTAGACTCG 
AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG CTGGAGCTGG TGATAGCTGG CACCGCGGAA 
ATCGACCTAA ATGACTGGCG GAATAACACT GAGTACCGGG GAGGTTACCA CGATGGGCAT 
CTTGTGATCC GCTGGTTCTG GGCTGCGGTG GAGCGCTTCA ATAATGAGCA GAGGCTGAGA 
TTACTGCAGT TTGTCACGGG AACATCCAGC GTGCCCTACG AAGGCTTCGC AGCCCTCCGT 
GGGAGCAATG GGCTTCGGCG CTTCTGCATA GAGAAATGGG GGAAAATTAC TTCTCTCCCC 
AGGGCACACA CATGCTTCAA CCGACTGGAT CTTCCACCGT ATCCCTCGTA CTCCATGTTG 
TATGAAAAGC TGTTAACAGC AGTAGAGGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513528.1
CDS	370..4683
Translation	MAALAEHKDDCISKEIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_011515226.3
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515226.3

ORF Insert Sequence:

ATGGCTGCTC TGGCAGAACA CAAAGATGAC TGCATTTCCA AGGAGATTTT TAAAAGCATT 
GGTGCTGATG AGACCGTCCA AGGACAAGGA AGTCGGAGGC TGATCAGCTT CTCTCTCTCA 
GATTTCCAAG CCATGGGGTT GAAGAAAGGG ATGTTTTTCA ACCCAGACCC TTATCTGAAG 
ATTTCCATTC AGCCTGGGAA ACACAGCATC TTCCCCGCCC TCCCTCACCA TGGACAGGAG 
AGGAGATCCA AGATCATAGG CAACACCGTG AACCCCATCT GGCAGGCCGA GCAATTCAGT 
TTTGTGTCCT TGCCCACTGA CGTGCTGGAA ATTGAGGTGA AGGACAAGTT TGCCAAGAGC 
CGCCCCATCA TCAAGCGCTT CTTGGGAAAG CTGTCGATGC CCGTTCAAAG ACTCCTGGAG 
AGACACGCCA TAGGGGATAG GGTGGTCAGC TACACACTTG GCCGCAGGCT TCCAACAGAT 
CATGTGAGTG GACAGCTGCA ATTCCGATTT GAGATCACTT CCTCCATCCA CCCAGCAGAT 
GATGAGGAGA TTTCCCTGAG TACCGAGCCT GAGTCAGCCC AAATTCAGGA CAGCCCCATG 
AACAACCTGA TGGAAAGCGG CAGTGGGGAA CCTCGGTCTG AGGCACCAGA GTCCTCTGAG 
AGCTGGAAGC CAGAGCAGCT GGGTGAGGGC AGTGTCCCCG ATGGTCCAGG GAACCAAAGC 
ATAGAGCTTT CCAGACCAGC TGAGGAAGCA GCAGTCATCA CGGAGGCAGG AGACCAGGGC 
ATGGTCTCTG TGGGACCTGA AGGGGCTGGG GAGCTCCTGG CCCAGGTGCA AAAGGACATC 
CAGCCTGCCC CCAGTGCAGA AGAGCTGGCC GAGCAGCTGG ACCTGGGTGA GGAGGCATCA 
GCACTGCTGC TGGAAGACGG TGAAGCCCCA GCCAGCACCA AGGAGGAGCC CTTGGAGGAG 
GAAGCAACGA CCCAGAGCCG GGCTGGAAGG GAAGAAGAGG AGAAGGAGCA GGAGGAGGAG 
GGAGATGTGT CTACCCTGGA GCAGGGAGAG GGCAGGCTGC AGCTGCGGGC CTCGGTGAAG 
AGAAAAAGCA GGCCCTGCTC CTTGCCTGTG TCCGAGCTGG AGACGGTGAT CGCGTCAGCC 
TGCGGGGACC CCGAGACCCC GCGGACACAC TACATCCGCA TCCACACCCT GCTGCACAGC 
ATGCCCTCCG CCCAGGGCGG CAGCGCGGCA GAGGAGGAGG ACGGCGCGGA GGAGGAGTCC 
ACCCTCAAGG ACTCCTCGGA GAAGGATGGG CTCAGCGAGG TGGACACGGT GGCCGCTGAC 
CCGTCTGCCC TGGAAGAGGA CAGAGAAGAG CCCGAGGGGG CTACTCCAGG CACGGCGCAC 
CCTGGCCACT CCGGGGGCCA CTTCCCCAGC CTGGCCAATG GCGCGGCCCA GGATGGCGAC 
ACGCACCCCA GCACCGGGAG CGAGAGCGAC TCCAGCCCCA GGCAAGGCGG GGACCACAGT 
TGCGAGGGCT GTGACGCGTC CTGCTGCAGC CCCTCGTGCT ACAGCTCCTC GTGCTACAGC 
ACGTCCTGCT ACAGCAGCTC GTGCTACAGC GCCTCGTGCT ACAGCCCCTC CTGCTACAAC 
GGCAACAGGT TCGCCAGCCA CACGCGCTTC TCCTCCGTGG ACAGCGCCAA GATCTCCGAG 
AGCACGGTCT TCTCCTCGCA AGACGACGAG GAGGAGGAGA ACAGCGCGTT CGAGTCGGTA 
CCCGACTCCA TGCAGAGCCC TGAGCTGGAC CCGGAGTCCA CGAACGGCGC TGGGCCGTGG 
CAAGACGAGC TGGCCGCCCC TAGCGGGCAC GTGGAAAGAA GCCCGGAAGG TCTGGAATCC 
CCCGTGGCAG GTCCAAGCAA TCGGAGAGAA GGTGAATGTC CTATACTCCA TAATTCCCAG 
CCAGTAAGCC AGCTTCCTTC CCTGAGGCCT GAACATCATC ACTACCCAAC AATCGATGAG 
CCTCTTCCAC CAAACTGGGA AGCTCGAATT GACAGCCACG GGCGGGTCTT TTATGTGGAC 
CACGTGAACC GCACAACCAC CTGGCAGCGT CCGACGGCAG CAGCCACCCC GGATGGCATG 
CGGAGATCGG GGTCCATCCA GCAGATGGAG CAACTCAACA GGCGGTATCA AAACATTCAG 
CGAACCATTG CAACAGAGAG GTCCGAAGAA GATTCTGGCA GCCAAAGCTG CGAGCAAGCC 
CCAGCAGGAG GAGGCGGAGG TGGAGGGAGT GACTCAGAAG CCGAATCTTC CCAGTCCAGC 
TTAGATCTAA GGAGAGAAGG GTCACTTTCT CCAGTGAACT CACAAAAAAT CACCTTGCTG 
CTGCAGTCCC CAGCGGTCAA GTTCATCACC AACCCCGAGT TCTTCACTGT GCTACATGCC 
AATTATAGTG CCTACCGAGT CTTCACCAGT AGCACCTGCT TAAAGCACAT GATTCTGAAA 
GTCCGACGGG ATGCTCGCAA TTTTGAACGC TACCAGCACA ACCGGGACTT GGTGAATTTC 
ATCAACATGT TCGCAGACAC TCGGCTGGAA CTGCCCCGGG GCTGGGAGAT CAAAACGGAC 
CAGCAGGGAA AGTCTTTTTT CGTGGACCAC AACAGTCGAG CTACCACTTT CATTGACCCC 
CGAATCCCTC TTCAGAACGG TCGTCTTCCC AATCATCTAA CTCACCGACA GCACCTCCAG 
AGGCTCCGAA GTTACAGCGC TGGAGAGGCC TCAGAAGTTT CTAGAAACAG AGGAGCCTCT 
TTACTGGCCA GGCCAGGACA CAGCTTAGTA GCTGCTATTC GAAGCCAACA TCAACATGAG 
TCATTGCCAC TGGCATATAA TGACAAGATT GTGGCATTTC TTCGCCAGCC AAACATTTTT 
GAAATGCTGC AAGAGCGTCA GCCAAGCTTA GCAAGAAACC ACACACTCAG GGAGAAAATC 
CATTACATTC GGACTGAGGG TAATCACGGG CTTGAGAAGT TGTCCTGTGA TGCGGATCTG 
GTCATTTTGC TGAGTCTCTT TGAAGAAGAG ATTATGTCCT ACGTCCCCCT GCAGGCTGCC 
TTCCACCCTG GGTATAGCTT CTCTCCCCGA TGTTCACCCT GTTCTTCACC TCAGAACTCC 
CCAGGTTTAC AGAGAGCCAG TGCAAGAGCC CCTTCCCCCT ACCGAAGAGA CTTTGAGGCC 
AAGCTCCGCA ATTTCTACAG AAAACTGGAA GCCAAAGGAT TTGGTCAGGG TCCGGGGAAA 
ATTAAGCTCA TTATTCGCCG GGATCATTTG TTGGAGGGAA CCTTCAATCA GGTGATGGCC 
TATTCGCGGA AAGAGCTCCA GCGAAACAAG CTCTACGTCA CCTTTGTTGG AGAGGAGGGC 
CTGGACTACA GTGGCCCCTC GCGGGAGTTC TTCTTCCTTC TGTCTCAGGA GCTCTTCAAC 
CCTTACTATG GACTCTTTGA GTACTCGGCA AATGATACTT ACACGGTGCA GATCAGCCCC 
ATGTCCGCAT TTGTAGAAAA CCATCTTGAG TGGTTCAGGT TTAGCGGTCG CATCCTGGGT 
CTGGCTCTGA TCCATCAGTA CCTTCTTGAC GCTTTCTTCA CGAGGCCCTT CTACAAGGCA 
CTCCTGAGAC TGCCCTGTGA TTTGAGTGAC CTGGAATATT TGGATGAGGA ATTCCACCAG 
AGTTTGCAGT GGATGAAGGA CAACAACATC ACAGACATCT TAGACCTCAC TTTCACTGTT 
AATGAAGAGG TTTTTGGACA GGTCACGGAA AGGGAGTTGA AGTCTGGAGG AGCCAACACA 
CAGGTGACGG AGAAAAACAA GAAGGAGTAC ATCGAGCGCA TGGTGAAGTG GCGGGTGGAG 
CGCGGCGTGG TACAGCAGAC CGAGGCGCTG GTGCGCGGCT TCTACGAGGT TGTAGACTCG 
AGGCTGGTGT CCGTGTTTGA TGCCAGGGAG CTGGAGCTGG TGATAGCTGG CACCGCGGAA 
ATCGACCTAA ATGACTGGCG GAATAACACT GAGTACCGGG GAGGTTACCA CGATGGGCAT 
CTTGTGATCC GCTGGTTCTG GGCTGCGGTG GAGCGCTTCA ATAATGAGCA GAGGCTGAGA 
TTACTGCAGT TTGTCACGGG AACATCCAGC GTGCCCTACG AAGGCTTCGC AGCCCTCCGT 
GGGAGCAATG GGCTTCGGCG CTTCTGCATA GAGAAATGGG GGAAAATTAC TTCTCTCCCC 
AGGGCACACA CATGCTTCAA CCGACTGGAT CTTCCACCGT ATCCCTCGTA CTCCATGTTG 
TATGAAAAGC TGTTAACAGC AGTAGAGGAA ACCAGCACCT TTGGACTTGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017011883.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867372.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu97114
Clone ID Related Accession (Same CDS sequence)	XM_017011883.2 , XM_017011883.1 , XM_017011882.1
Accession Version	XM_017011883.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011883.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTACC CGAAAAGGCC AACCGTTAAA GACCCCGGCA GTGTTGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC ATCAGCAGAC TCACTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCCGCCA TGGCGTCTCC TTCTAGAAAC 
TCCCAGAGCC GACGCCGGTG CAAGGAGCCG CTCCGATACA GCTACAACCC CGACCAGTTC 
CACAACATGG ACCTCAGGGG CGGCCCCCAC GATGGCGTCA CCATTCCCCG CTCCACCAGC 
GACACTGACC TGGTCACCTC GGACAGCCGC TCCACGCTCA TGGTCAGCAG CTCCTACTAT 
TCCATCGGGC ACTCTCAGGA CCTGGTCATC CACTGGGACA TAAAGGAGGA AGTGGACGCT 
GGGGACTGGA TTGGCATGTA CCTCATTGAT GAGGTCTTGT CCGAAAACTT TCTGGACTAT 
AAAAACCGTG GAGTCAATGG TTCTCATCGG GGCCAGATCA TCTGGAAGAT CGATGCCAGC 
TCGTACTTTG TGGAACCTGA AACTAAGATC TGCTTCAAAT ACTACCATGG AGTGAGTGGG 
GCCCTGCGAG CAACCACCCC CAGTGTCACG GTCAAAAACT CGGCAGCTCC TATTTTTAAA 
AGCATTGGTG CTGATGAGAC CGTCCAAGGA CAAGGAAGTC GGAGGCTGAT CAGCTTCTCT 
CTCTCAGATT TCCAAGCCAT GGGGTTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAT 
CTGAAGATTT CCATTCAGCC TGGGAAACAC AGCATCTTCC CCGCCCTCCC TCACCATGGA 
CAGGAGAGGA GATCCAAGAT CATAGGCAAC ACCGTGAACC CCATCTGGCA GGCCGAGCAA 
TTCAGTTTTG TGTCCTTGCC CACTGACGTG CTGGAAATTG AGGTGAAGGA CAAGTTTGCC 
AAGAGCCGCC CCATCATCAA GCGCTTCTTG GGAAAGCTGT CGATGCCCGT TCAAAGACTC 
CTGGAGAGAC ACGCCATAGG GGATAGGGTG GTCAGCTACA CACTTGGCCG CAGGCTTCCA 
ACAGATCATG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCCTC CATCCACCCA 
GCAGATGATG AGGAGATTTC CCTGAGTACC GAGCCTGAGT CAGCCCAAAT TCAGGACAGC 
CCCATGAACA ACCTGATGGA AAGCGGCAGT GGGGAACCTC GGTCTGAGGC ACCAGAGTCC 
TCTGAGAGCT GGAAGCCAGA GCAGCTGGGT GAGGGCAGTG TCCCCGATGG TCCAGGGAAC 
CAAAGCATAG AGCTTTCCAG ACCAGCTGAG GAAGCAGCAG TCATCACGGA GGCAGGAGAC 
CAGGGCATGG TCTCTGTGGG ACCTGAAGGG GCTGGGGAGC TCCTGGCCCA GGTGCAAAAG 
GACATCCAGC CTGCCCCCAG TGCAGAAGAG CTGGCCGAGC AGCTGGACCT GGGTGAGGAG 
GCATCAGCAC TGCTGCTGGA AGACGGTGAA GCCCCAGCCA GCACCAAGGA GGAGCCCTTG 
GAGGAGGAAG CAACGACCCA GAGCCGGGCT GGAAGGGAAG AAGAGGAGAA GGAGCAGGAG 
GAGGAGGGAG ATGTGTCTAC CCTGGAGCAG GGAGAGGGCA GGCTGCAGCT GCGGGCCTCG 
GTGAAGAGAA AAAGCAGGCC CTGCTCCTTG CCTGTGTCCG AGCTGGAGAC GGTGATCGCG 
TCAGCCTGCG GGGACCCCGA GACCCCGCGG ACACACTACA TCCGCATCCA CACCCTGCTG 
CACAGCATGC CCTCCGCCCA GGGCGGCAGC GCGGCAGAGG AGGAGGACGG CGCGGAGGAG 
GAGTCCACCC TCAAGGACTC CTCGGAGAAG GATGGGCTCA GCGAGGTGGA CACGGTGGCC 
GCTGACCCGT CTGCCCTGGA AGAGGACAGA GAAGAGCCCG AGGGGGCTAC TCCAGGCACG 
GCGCACCCTG GCCACTCCGG GGGCCACTTC CCCAGCCTGG CCAATGGCGC GGCCCAGGAT 
GGCGACACGC ACCCCAGCAC CGGGAGCGAG AGCGACTCCA GCCCCAGGCA AGGCGGGGAC 
CACAGTTGCG AGGGCTGTGA CGCGTCCTGC TGCAGCCCCT CGTGCTACAG CTCCTCGTGC 
TACAGCACGT CCTGCTACAG CAGCTCGTGC TACAGCGCCT CGTGCTACAG CCCCTCCTGC 
TACAACGGCA ACAGGTTCGC CAGCCACACG CGCTTCTCCT CCGTGGACAG CGCCAAGATC 
TCCGAGAGCA CGGTCTTCTC CTCGCAAGAC GACGAGGAGG AGGAGAACAG CGCGTTCGAG 
TCGGTACCCG ACTCCATGCA GAGCCCTGAG CTGGACCCGG AGTCCACGAA CGGCGCTGGG 
CCGTGGCAAG ACGAGCTGGC CGCCCCTAGC GGGCACGTGG AAAGAAGCCC GGAAGGTCTG 
GAATCCCCCG TGGCAGGTCC AAGCAATCGG AGAGAAGGTG AATGTCCTAT ACTCCATAAT 
TCCCAGCCAG TAAGCCAGCT TCCTTCCCTG AGGCCTGAAC ATCATCACTA CCCAACAATC 
GATGAGCCTC TTCCACCAAA CTGGGAAGCT CGAATTGACA GCCACGGGCG GGTCTTTTAT 
GTGGACCACG TGAACCGCAC AACCACCTGG CAGCGTCCGA CGGCAGCAGC CACCCCGGAT 
GGCATGCGGA GATCGGGGTC CATCCAGCAG ATGGAGCAAC TCAACAGGCG GTATCAAAAC 
ATTCAGCGAA CCATTGCAAC AGAGAGGTCC GAAGAAGATT CTGGCAGCCA AAGCTGCGAG 
CAAGCCCCAG CAGGAGGAGG CGGAGGTGGA GGGAGTGACT CAGAAGCCGA ATCTTCCCAG 
TCCAGCTTAG ATCTAAGGAG AGAAGGGTCA CTTTCTCCAG TGAACTCACA AAAAATCACC 
TTGCTGCTGC AGTCCCCAGC GGTCAAGTTC ATCACCAACC CCGAGTTCTT CACTGTGCTA 
CATGCCAATT ATAGTGCCTA CCGAGTCTTC ACCAGTAGCA CCTGCTTAAA GCACATGATT 
CTGAAAGTCC GACGGGATGC TCGCAATTTT GAACGCTACC AGCACAACCG GGACTTGGTG 
AATTTCATCA ACATGTTCGC AGACACTCGG CTGGAACTGC CCCGGGGCTG GGAGATCAAA 
ACGGACCAGC AGGGAAAGTC TTTTTTCGTG GACCACAACA GTCGAGCTAC CACTTTCATT 
GACCCCCGAA TCCCTCTTCA GAACGGTCGT CTTCCCAATC ATCTAACTCA CCGACAGCAC 
CTCCAGAGGC TCCGAAGTTA CAGCGCTGGA GAGGCCTCAG AAGTTTCTAG AAACAGAGGA 
GCCTCTTTAC TGGCCAGGCC AGGACACAGC TTAGTAGCTG CTATTCGAAG CCAACATCAA 
CATGAGTCAT TGCCACTGGC ATATAATGAC AAGATTGTGG CATTTCTTCG CCAGCCAAAC 
ATTTTTGAAA TGCTGCAAGA GCGTCAGCCA AGCTTAGCAA GAAACCACAC ACTCAGGGAG 
AAAATCCATT ACATTCGGAC TGAGGGTAAT CACGGGCTTG AGAAGTTGTC CTGTGATGCG 
GATCTGGTCA TTTTGCTGAG TCTCTTTGAA GAAGAGATTA TGTCCTACGT CCCCCTGCAG 
GCTGCCTTCC ACCCTGGGTA TAGCTTCTCT CCCCGATGTT CACCCTGTTC TTCACCTCAG 
AACTCCCCAG GTTTACAGAG AGCCAGTGCA AGAGCCCCTT CCCCCTACCG AAGAGACTTT 
GAGGCCAAGC TCCGCAATTT CTACAGAAAA CTGGAAGCCA AAGGATTTGG TCAGGGTCCG 
GGGAAAATTA AGCTCATTAT TCGCCGGGAT CATTTGTTGG AGGGAACCTT CAATCAGGTG 
ATGGCCTATT CGCGGAAAGA GCTCCAGCGA AACAAGCTCT ACGTCACCTT TGTTGGAGAG 
GAGGGCCTGG ACTACAGTGG CCCCTCGCGG GAGTTCTTCT TCCTTCTGTC TCAGGAGCTC 
TTCAACCCTT ACTATGGACT CTTTGAGTAC TCGGCAAATG ATACTTACAC GGTGCAGATC 
AGCCCCATGT CCGCATTTGT AGAAAACCAT CTTGAGTGGT TCAGGTTTAG CGGTCGCATC 
CTGGGTCTGG CTCTGATCCA TCAGTACCTT CTTGACGCTT TCTTCACGAG GCCCTTCTAC 
AAGGCACTCC TGAGACTGCC CTGTGATTTG AGTGACCTGG AATATTTGGA TGAGGAATTC 
CACCAGAGTT TGCAGTGGAT GAAGGACAAC AACATCACAG ACATCTTAGA CCTCACTTTC 
ACTGTTAATG AAGAGGTTTT TGGACAGGTC ACGGAAAGGG AGTTGAAGTC TGGAGGAGCC 
AACACACAGG TGACGGAGAA AAACAAGAAG GAGTACATCG AGCGCATGGT GAAGTGGCGG 
GTGGAGCGCG GCGTGGTACA GCAGACCGAG GCGCTGGTGC GCGGCTTCTA CGAGGTTGTA 
GACTCGAGGC TGGTGTCCGT GTTTGATGCC AGGGAGCTGG AGCTGGTGAT AGCTGGCACC 
GCGGAAATCG ACCTAAATGA CTGGCGGAAT AACACTGAGT ACCGGGGAGG TTACCACGAT 
GGGCATCTTG TGATCCGCTG GTTCTGGGCT GCGGTGGAGC GCTTCAATAA TGAGCAGAGG 
CTGAGATTAC TGCAGTTTGT CACGGGAACA TCCAGCGTGC CCTACGAAGG CTTCGCAGCC 
CTCCGTGGGA GCAATGGGCT TCGGCGCTTC TGCATAGAGA AATGGGGGAA AATTACTTCT 
CTCCCCAGGG CACACACATG CTTCAACCGA CTGGATCTTC CACCGTATCC CTCGTACTCC 
ATGTTGTATG AAAAGCTGTT AACAGCAGTA GAGGAAACCA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867372.1
CDS	201..5120
Translation	MPYPKRPTVKDPGSVVVQGVSKQVARSAFLISRLTPAVAITNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017011883.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011883.2

ORF Insert Sequence:

ATGCCCTACC CGAAAAGGCC AACCGTTAAA GACCCCGGCA GTGTTGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC ATCAGCAGAC TCACTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCCGCCA TGGCGTCTCC TTCTAGAAAC 
TCCCAGAGCC GACGCCGGTG CAAGGAGCCG CTCCGATACA GCTACAACCC CGACCAGTTC 
CACAACATGG ACCTCAGGGG CGGCCCCCAC GATGGCGTCA CCATTCCCCG CTCCACCAGC 
GACACTGACC TGGTCACCTC GGACAGCCGC TCCACGCTCA TGGTCAGCAG CTCCTACTAT 
TCCATCGGGC ACTCTCAGGA CCTGGTCATC CACTGGGACA TAAAGGAGGA AGTGGACGCT 
GGGGACTGGA TTGGCATGTA CCTCATTGAT GAGGTCTTGT CCGAAAACTT TCTGGACTAT 
AAAAACCGTG GAGTCAATGG TTCTCATCGG GGCCAGATCA TCTGGAAGAT CGATGCCAGC 
TCGTACTTTG TGGAACCTGA AACTAAGATC TGCTTCAAAT ACTACCATGG AGTGAGTGGG 
GCCCTGCGAG CAACCACCCC CAGTGTCACG GTCAAAAACT CGGCAGCTCC TATTTTTAAA 
AGCATTGGTG CTGATGAGAC CGTCCAAGGA CAAGGAAGTC GGAGGCTGAT CAGCTTCTCT 
CTCTCAGATT TCCAAGCCAT GGGGTTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAT 
CTGAAGATTT CCATTCAGCC TGGGAAACAC AGCATCTTCC CCGCCCTCCC TCACCATGGA 
CAGGAGAGGA GATCCAAGAT CATAGGCAAC ACCGTGAACC CCATCTGGCA GGCCGAGCAA 
TTCAGTTTTG TGTCCTTGCC CACTGACGTG CTGGAAATTG AGGTGAAGGA CAAGTTTGCC 
AAGAGCCGCC CCATCATCAA GCGCTTCTTG GGAAAGCTGT CGATGCCCGT TCAAAGACTC 
CTGGAGAGAC ACGCCATAGG GGATAGGGTG GTCAGCTACA CACTTGGCCG CAGGCTTCCA 
ACAGATCATG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCCTC CATCCACCCA 
GCAGATGATG AGGAGATTTC CCTGAGTACC GAGCCTGAGT CAGCCCAAAT TCAGGACAGC 
CCCATGAACA ACCTGATGGA AAGCGGCAGT GGGGAACCTC GGTCTGAGGC ACCAGAGTCC 
TCTGAGAGCT GGAAGCCAGA GCAGCTGGGT GAGGGCAGTG TCCCCGATGG TCCAGGGAAC 
CAAAGCATAG AGCTTTCCAG ACCAGCTGAG GAAGCAGCAG TCATCACGGA GGCAGGAGAC 
CAGGGCATGG TCTCTGTGGG ACCTGAAGGG GCTGGGGAGC TCCTGGCCCA GGTGCAAAAG 
GACATCCAGC CTGCCCCCAG TGCAGAAGAG CTGGCCGAGC AGCTGGACCT GGGTGAGGAG 
GCATCAGCAC TGCTGCTGGA AGACGGTGAA GCCCCAGCCA GCACCAAGGA GGAGCCCTTG 
GAGGAGGAAG CAACGACCCA GAGCCGGGCT GGAAGGGAAG AAGAGGAGAA GGAGCAGGAG 
GAGGAGGGAG ATGTGTCTAC CCTGGAGCAG GGAGAGGGCA GGCTGCAGCT GCGGGCCTCG 
GTGAAGAGAA AAAGCAGGCC CTGCTCCTTG CCTGTGTCCG AGCTGGAGAC GGTGATCGCG 
TCAGCCTGCG GGGACCCCGA GACCCCGCGG ACACACTACA TCCGCATCCA CACCCTGCTG 
CACAGCATGC CCTCCGCCCA GGGCGGCAGC GCGGCAGAGG AGGAGGACGG CGCGGAGGAG 
GAGTCCACCC TCAAGGACTC CTCGGAGAAG GATGGGCTCA GCGAGGTGGA CACGGTGGCC 
GCTGACCCGT CTGCCCTGGA AGAGGACAGA GAAGAGCCCG AGGGGGCTAC TCCAGGCACG 
GCGCACCCTG GCCACTCCGG GGGCCACTTC CCCAGCCTGG CCAATGGCGC GGCCCAGGAT 
GGCGACACGC ACCCCAGCAC CGGGAGCGAG AGCGACTCCA GCCCCAGGCA AGGCGGGGAC 
CACAGTTGCG AGGGCTGTGA CGCGTCCTGC TGCAGCCCCT CGTGCTACAG CTCCTCGTGC 
TACAGCACGT CCTGCTACAG CAGCTCGTGC TACAGCGCCT CGTGCTACAG CCCCTCCTGC 
TACAACGGCA ACAGGTTCGC CAGCCACACG CGCTTCTCCT CCGTGGACAG CGCCAAGATC 
TCCGAGAGCA CGGTCTTCTC CTCGCAAGAC GACGAGGAGG AGGAGAACAG CGCGTTCGAG 
TCGGTACCCG ACTCCATGCA GAGCCCTGAG CTGGACCCGG AGTCCACGAA CGGCGCTGGG 
CCGTGGCAAG ACGAGCTGGC CGCCCCTAGC GGGCACGTGG AAAGAAGCCC GGAAGGTCTG 
GAATCCCCCG TGGCAGGTCC AAGCAATCGG AGAGAAGGTG AATGTCCTAT ACTCCATAAT 
TCCCAGCCAG TAAGCCAGCT TCCTTCCCTG AGGCCTGAAC ATCATCACTA CCCAACAATC 
GATGAGCCTC TTCCACCAAA CTGGGAAGCT CGAATTGACA GCCACGGGCG GGTCTTTTAT 
GTGGACCACG TGAACCGCAC AACCACCTGG CAGCGTCCGA CGGCAGCAGC CACCCCGGAT 
GGCATGCGGA GATCGGGGTC CATCCAGCAG ATGGAGCAAC TCAACAGGCG GTATCAAAAC 
ATTCAGCGAA CCATTGCAAC AGAGAGGTCC GAAGAAGATT CTGGCAGCCA AAGCTGCGAG 
CAAGCCCCAG CAGGAGGAGG CGGAGGTGGA GGGAGTGACT CAGAAGCCGA ATCTTCCCAG 
TCCAGCTTAG ATCTAAGGAG AGAAGGGTCA CTTTCTCCAG TGAACTCACA AAAAATCACC 
TTGCTGCTGC AGTCCCCAGC GGTCAAGTTC ATCACCAACC CCGAGTTCTT CACTGTGCTA 
CATGCCAATT ATAGTGCCTA CCGAGTCTTC ACCAGTAGCA CCTGCTTAAA GCACATGATT 
CTGAAAGTCC GACGGGATGC TCGCAATTTT GAACGCTACC AGCACAACCG GGACTTGGTG 
AATTTCATCA ACATGTTCGC AGACACTCGG CTGGAACTGC CCCGGGGCTG GGAGATCAAA 
ACGGACCAGC AGGGAAAGTC TTTTTTCGTG GACCACAACA GTCGAGCTAC CACTTTCATT 
GACCCCCGAA TCCCTCTTCA GAACGGTCGT CTTCCCAATC ATCTAACTCA CCGACAGCAC 
CTCCAGAGGC TCCGAAGTTA CAGCGCTGGA GAGGCCTCAG AAGTTTCTAG AAACAGAGGA 
GCCTCTTTAC TGGCCAGGCC AGGACACAGC TTAGTAGCTG CTATTCGAAG CCAACATCAA 
CATGAGTCAT TGCCACTGGC ATATAATGAC AAGATTGTGG CATTTCTTCG CCAGCCAAAC 
ATTTTTGAAA TGCTGCAAGA GCGTCAGCCA AGCTTAGCAA GAAACCACAC ACTCAGGGAG 
AAAATCCATT ACATTCGGAC TGAGGGTAAT CACGGGCTTG AGAAGTTGTC CTGTGATGCG 
GATCTGGTCA TTTTGCTGAG TCTCTTTGAA GAAGAGATTA TGTCCTACGT CCCCCTGCAG 
GCTGCCTTCC ACCCTGGGTA TAGCTTCTCT CCCCGATGTT CACCCTGTTC TTCACCTCAG 
AACTCCCCAG GTTTACAGAG AGCCAGTGCA AGAGCCCCTT CCCCCTACCG AAGAGACTTT 
GAGGCCAAGC TCCGCAATTT CTACAGAAAA CTGGAAGCCA AAGGATTTGG TCAGGGTCCG 
GGGAAAATTA AGCTCATTAT TCGCCGGGAT CATTTGTTGG AGGGAACCTT CAATCAGGTG 
ATGGCCTATT CGCGGAAAGA GCTCCAGCGA AACAAGCTCT ACGTCACCTT TGTTGGAGAG 
GAGGGCCTGG ACTACAGTGG CCCCTCGCGG GAGTTCTTCT TCCTTCTGTC TCAGGAGCTC 
TTCAACCCTT ACTATGGACT CTTTGAGTAC TCGGCAAATG ATACTTACAC GGTGCAGATC 
AGCCCCATGT CCGCATTTGT AGAAAACCAT CTTGAGTGGT TCAGGTTTAG CGGTCGCATC 
CTGGGTCTGG CTCTGATCCA TCAGTACCTT CTTGACGCTT TCTTCACGAG GCCCTTCTAC 
AAGGCACTCC TGAGACTGCC CTGTGATTTG AGTGACCTGG AATATTTGGA TGAGGAATTC 
CACCAGAGTT TGCAGTGGAT GAAGGACAAC AACATCACAG ACATCTTAGA CCTCACTTTC 
ACTGTTAATG AAGAGGTTTT TGGACAGGTC ACGGAAAGGG AGTTGAAGTC TGGAGGAGCC 
AACACACAGG TGACGGAGAA AAACAAGAAG GAGTACATCG AGCGCATGGT GAAGTGGCGG 
GTGGAGCGCG GCGTGGTACA GCAGACCGAG GCGCTGGTGC GCGGCTTCTA CGAGGTTGTA 
GACTCGAGGC TGGTGTCCGT GTTTGATGCC AGGGAGCTGG AGCTGGTGAT AGCTGGCACC 
GCGGAAATCG ACCTAAATGA CTGGCGGAAT AACACTGAGT ACCGGGGAGG TTACCACGAT 
GGGCATCTTG TGATCCGCTG GTTCTGGGCT GCGGTGGAGC GCTTCAATAA TGAGCAGAGG 
CTGAGATTAC TGCAGTTTGT CACGGGAACA TCCAGCGTGC CCTACGAAGG CTTCGCAGCC 
CTCCGTGGGA GCAATGGGCT TCGGCGCTTC TGCATAGAGA AATGGGGGAA AATTACTTCT 
CTCCCCAGGG CACACACATG CTTCAACCGA CTGGATCTTC CACCGTATCC CTCGTACTCC 
ATGTTGTATG AAAAGCTGTT AACAGCAGTA GAGGAAACCA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017011883.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867372.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

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CloneID	OHu97114
Clone ID Related Accession (Same CDS sequence)	XM_017011883.2 , XM_017011883.1 , XM_017011882.1
Accession Version	XM_017011883.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTACC CGAAAAGGCC AACCGTTAAA GACCCCGGCA GTGTTGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC ATCAGCAGAC TCACTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCCGCCA TGGCGTCTCC TTCTAGAAAC 
TCCCAGAGCC GACGCCGGTG CAAGGAGCCG CTCCGATACA GCTACAACCC CGACCAGTTC 
CACAACATGG ACCTCAGGGG CGGCCCCCAC GATGGCGTCA CCATTCCCCG CTCCACCAGC 
GACACTGACC TGGTCACCTC GGACAGCCGC TCCACGCTCA TGGTCAGCAG CTCCTACTAT 
TCCATCGGGC ACTCTCAGGA CCTGGTCATC CACTGGGACA TAAAGGAGGA AGTGGACGCT 
GGGGACTGGA TTGGCATGTA CCTCATTGAT GAGGTCTTGT CCGAAAACTT TCTGGACTAT 
AAAAACCGTG GAGTCAATGG TTCTCATCGG GGCCAGATCA TCTGGAAGAT CGATGCCAGC 
TCGTACTTTG TGGAACCTGA AACTAAGATC TGCTTCAAAT ACTACCATGG AGTGAGTGGG 
GCCCTGCGAG CAACCACCCC CAGTGTCACG GTCAAAAACT CGGCAGCTCC TATTTTTAAA 
AGCATTGGTG CTGATGAGAC CGTCCAAGGA CAAGGAAGTC GGAGGCTGAT CAGCTTCTCT 
CTCTCAGATT TCCAAGCCAT GGGGTTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAT 
CTGAAGATTT CCATTCAGCC TGGGAAACAC AGCATCTTCC CCGCCCTCCC TCACCATGGA 
CAGGAGAGGA GATCCAAGAT CATAGGCAAC ACCGTGAACC CCATCTGGCA GGCCGAGCAA 
TTCAGTTTTG TGTCCTTGCC CACTGACGTG CTGGAAATTG AGGTGAAGGA CAAGTTTGCC 
AAGAGCCGCC CCATCATCAA GCGCTTCTTG GGAAAGCTGT CGATGCCCGT TCAAAGACTC 
CTGGAGAGAC ACGCCATAGG GGATAGGGTG GTCAGCTACA CACTTGGCCG CAGGCTTCCA 
ACAGATCATG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCCTC CATCCACCCA 
GCAGATGATG AGGAGATTTC CCTGAGTACC GAGCCTGAGT CAGCCCAAAT TCAGGACAGC 
CCCATGAACA ACCTGATGGA AAGCGGCAGT GGGGAACCTC GGTCTGAGGC ACCAGAGTCC 
TCTGAGAGCT GGAAGCCAGA GCAGCTGGGT GAGGGCAGTG TCCCCGATGG TCCAGGGAAC 
CAAAGCATAG AGCTTTCCAG ACCAGCTGAG GAAGCAGCAG TCATCACGGA GGCAGGAGAC 
CAGGGCATGG TCTCTGTGGG ACCTGAAGGG GCTGGGGAGC TCCTGGCCCA GGTGCAAAAG 
GACATCCAGC CTGCCCCCAG TGCAGAAGAG CTGGCCGAGC AGCTGGACCT GGGTGAGGAG 
GCATCAGCAC TGCTGCTGGA AGACGGTGAA GCCCCAGCCA GCACCAAGGA GGAGCCCTTG 
GAGGAGGAAG CAACGACCCA GAGCCGGGCT GGAAGGGAAG AAGAGGAGAA GGAGCAGGAG 
GAGGAGGGAG ATGTGTCTAC CCTGGAGCAG GGAGAGGGCA GGCTGCAGCT GCGGGCCTCG 
GTGAAGAGAA AAAGCAGGCC CTGCTCCTTG CCTGTGTCCG AGCTGGAGAC GGTGATCGCG 
TCAGCCTGCG GGGACCCCGA GACCCCGCGG ACACACTACA TCCGCATCCA CACCCTGCTG 
CACAGCATGC CCTCCGCCCA GGGCGGCAGC GCGGCAGAGG AGGAGGACGG CGCGGAGGAG 
GAGTCCACCC TCAAGGACTC CTCGGAGAAG GATGGGCTCA GCGAGGTGGA CACGGTGGCC 
GCTGACCCGT CTGCCCTGGA AGAGGACAGA GAAGAGCCCG AGGGGGCTAC TCCAGGCACG 
GCGCACCCTG GCCACTCCGG GGGCCACTTC CCCAGCCTGG CCAATGGCGC GGCCCAGGAT 
GGCGACACGC ACCCCAGCAC CGGGAGCGAG AGCGACTCCA GCCCCAGGCA AGGCGGGGAC 
CACAGTTGCG AGGGCTGTGA CGCGTCCTGC TGCAGCCCCT CGTGCTACAG CTCCTCGTGC 
TACAGCACGT CCTGCTACAG CAGCTCGTGC TACAGCGCCT CGTGCTACAG CCCCTCCTGC 
TACAACGGCA ACAGGTTCGC CAGCCACACG CGCTTCTCCT CCGTGGACAG CGCCAAGATC 
TCCGAGAGCA CGGTCTTCTC CTCGCAAGAC GACGAGGAGG AGGAGAACAG CGCGTTCGAG 
TCGGTACCCG ACTCCATGCA GAGCCCTGAG CTGGACCCGG AGTCCACGAA CGGCGCTGGG 
CCGTGGCAAG ACGAGCTGGC CGCCCCTAGC GGGCACGTGG AAAGAAGCCC GGAAGGTCTG 
GAATCCCCCG TGGCAGGTCC AAGCAATCGG AGAGAAGGTG AATGTCCTAT ACTCCATAAT 
TCCCAGCCAG TAAGCCAGCT TCCTTCCCTG AGGCCTGAAC ATCATCACTA CCCAACAATC 
GATGAGCCTC TTCCACCAAA CTGGGAAGCT CGAATTGACA GCCACGGGCG GGTCTTTTAT 
GTGGACCACG TGAACCGCAC AACCACCTGG CAGCGTCCGA CGGCAGCAGC CACCCCGGAT 
GGCATGCGGA GATCGGGGTC CATCCAGCAG ATGGAGCAAC TCAACAGGCG GTATCAAAAC 
ATTCAGCGAA CCATTGCAAC AGAGAGGTCC GAAGAAGATT CTGGCAGCCA AAGCTGCGAG 
CAAGCCCCAG CAGGAGGAGG CGGAGGTGGA GGGAGTGACT CAGAAGCCGA ATCTTCCCAG 
TCCAGCTTAG ATCTAAGGAG AGAAGGGTCA CTTTCTCCAG TGAACTCACA AAAAATCACC 
TTGCTGCTGC AGTCCCCAGC GGTCAAGTTC ATCACCAACC CCGAGTTCTT CACTGTGCTA 
CATGCCAATT ATAGTGCCTA CCGAGTCTTC ACCAGTAGCA CCTGCTTAAA GCACATGATT 
CTGAAAGTCC GACGGGATGC TCGCAATTTT GAACGCTACC AGCACAACCG GGACTTGGTG 
AATTTCATCA ACATGTTCGC AGACACTCGG CTGGAACTGC CCCGGGGCTG GGAGATCAAA 
ACGGACCAGC AGGGAAAGTC TTTTTTCGTG GACCACAACA GTCGAGCTAC CACTTTCATT 
GACCCCCGAA TCCCTCTTCA GAACGGTCGT CTTCCCAATC ATCTAACTCA CCGACAGCAC 
CTCCAGAGGC TCCGAAGTTA CAGCGCTGGA GAGGCCTCAG AAGTTTCTAG AAACAGAGGA 
GCCTCTTTAC TGGCCAGGCC AGGACACAGC TTAGTAGCTG CTATTCGAAG CCAACATCAA 
CATGAGTCAT TGCCACTGGC ATATAATGAC AAGATTGTGG CATTTCTTCG CCAGCCAAAC 
ATTTTTGAAA TGCTGCAAGA GCGTCAGCCA AGCTTAGCAA GAAACCACAC ACTCAGGGAG 
AAAATCCATT ACATTCGGAC TGAGGGTAAT CACGGGCTTG AGAAGTTGTC CTGTGATGCG 
GATCTGGTCA TTTTGCTGAG TCTCTTTGAA GAAGAGATTA TGTCCTACGT CCCCCTGCAG 
GCTGCCTTCC ACCCTGGGTA TAGCTTCTCT CCCCGATGTT CACCCTGTTC TTCACCTCAG 
AACTCCCCAG GTTTACAGAG AGCCAGTGCA AGAGCCCCTT CCCCCTACCG AAGAGACTTT 
GAGGCCAAGC TCCGCAATTT CTACAGAAAA CTGGAAGCCA AAGGATTTGG TCAGGGTCCG 
GGGAAAATTA AGCTCATTAT TCGCCGGGAT CATTTGTTGG AGGGAACCTT CAATCAGGTG 
ATGGCCTATT CGCGGAAAGA GCTCCAGCGA AACAAGCTCT ACGTCACCTT TGTTGGAGAG 
GAGGGCCTGG ACTACAGTGG CCCCTCGCGG GAGTTCTTCT TCCTTCTGTC TCAGGAGCTC 
TTCAACCCTT ACTATGGACT CTTTGAGTAC TCGGCAAATG ATACTTACAC GGTGCAGATC 
AGCCCCATGT CCGCATTTGT AGAAAACCAT CTTGAGTGGT TCAGGTTTAG CGGTCGCATC 
CTGGGTCTGG CTCTGATCCA TCAGTACCTT CTTGACGCTT TCTTCACGAG GCCCTTCTAC 
AAGGCACTCC TGAGACTGCC CTGTGATTTG AGTGACCTGG AATATTTGGA TGAGGAATTC 
CACCAGAGTT TGCAGTGGAT GAAGGACAAC AACATCACAG ACATCTTAGA CCTCACTTTC 
ACTGTTAATG AAGAGGTTTT TGGACAGGTC ACGGAAAGGG AGTTGAAGTC TGGAGGAGCC 
AACACACAGG TGACGGAGAA AAACAAGAAG GAGTACATCG AGCGCATGGT GAAGTGGCGG 
GTGGAGCGCG GCGTGGTACA GCAGACCGAG GCGCTGGTGC GCGGCTTCTA CGAGGTTGTA 
GACTCGAGGC TGGTGTCCGT GTTTGATGCC AGGGAGCTGG AGCTGGTGAT AGCTGGCACC 
GCGGAAATCG ACCTAAATGA CTGGCGGAAT AACACTGAGT ACCGGGGAGG TTACCACGAT 
GGGCATCTTG TGATCCGCTG GTTCTGGGCT GCGGTGGAGC GCTTCAATAA TGAGCAGAGG 
CTGAGATTAC TGCAGTTTGT CACGGGAACA TCCAGCGTGC CCTACGAAGG CTTCGCAGCC 
CTCCGTGGGA GCAATGGGCT TCGGCGCTTC TGCATAGAGA AATGGGGGAA AATTACTTCT 
CTCCCCAGGG CACACACATG CTTCAACCGA CTGGATCTTC CACCGTATCC CTCGTACTCC 
ATGTTGTATG AAAAGCTGTT AACAGCAGTA GAGGAAACCA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867372.1
CDS	466..5385
Translation	MPYPKRPTVKDPGSVVVQGVSKQVARSAFLISRLTPAVAITNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017011883.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011883.1

ORF Insert Sequence:

ATGCCCTACC CGAAAAGGCC AACCGTTAAA GACCCCGGCA GTGTTGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC ATCAGCAGAC TCACTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCCGCCA TGGCGTCTCC TTCTAGAAAC 
TCCCAGAGCC GACGCCGGTG CAAGGAGCCG CTCCGATACA GCTACAACCC CGACCAGTTC 
CACAACATGG ACCTCAGGGG CGGCCCCCAC GATGGCGTCA CCATTCCCCG CTCCACCAGC 
GACACTGACC TGGTCACCTC GGACAGCCGC TCCACGCTCA TGGTCAGCAG CTCCTACTAT 
TCCATCGGGC ACTCTCAGGA CCTGGTCATC CACTGGGACA TAAAGGAGGA AGTGGACGCT 
GGGGACTGGA TTGGCATGTA CCTCATTGAT GAGGTCTTGT CCGAAAACTT TCTGGACTAT 
AAAAACCGTG GAGTCAATGG TTCTCATCGG GGCCAGATCA TCTGGAAGAT CGATGCCAGC 
TCGTACTTTG TGGAACCTGA AACTAAGATC TGCTTCAAAT ACTACCATGG AGTGAGTGGG 
GCCCTGCGAG CAACCACCCC CAGTGTCACG GTCAAAAACT CGGCAGCTCC TATTTTTAAA 
AGCATTGGTG CTGATGAGAC CGTCCAAGGA CAAGGAAGTC GGAGGCTGAT CAGCTTCTCT 
CTCTCAGATT TCCAAGCCAT GGGGTTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAT 
CTGAAGATTT CCATTCAGCC TGGGAAACAC AGCATCTTCC CCGCCCTCCC TCACCATGGA 
CAGGAGAGGA GATCCAAGAT CATAGGCAAC ACCGTGAACC CCATCTGGCA GGCCGAGCAA 
TTCAGTTTTG TGTCCTTGCC CACTGACGTG CTGGAAATTG AGGTGAAGGA CAAGTTTGCC 
AAGAGCCGCC CCATCATCAA GCGCTTCTTG GGAAAGCTGT CGATGCCCGT TCAAAGACTC 
CTGGAGAGAC ACGCCATAGG GGATAGGGTG GTCAGCTACA CACTTGGCCG CAGGCTTCCA 
ACAGATCATG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCCTC CATCCACCCA 
GCAGATGATG AGGAGATTTC CCTGAGTACC GAGCCTGAGT CAGCCCAAAT TCAGGACAGC 
CCCATGAACA ACCTGATGGA AAGCGGCAGT GGGGAACCTC GGTCTGAGGC ACCAGAGTCC 
TCTGAGAGCT GGAAGCCAGA GCAGCTGGGT GAGGGCAGTG TCCCCGATGG TCCAGGGAAC 
CAAAGCATAG AGCTTTCCAG ACCAGCTGAG GAAGCAGCAG TCATCACGGA GGCAGGAGAC 
CAGGGCATGG TCTCTGTGGG ACCTGAAGGG GCTGGGGAGC TCCTGGCCCA GGTGCAAAAG 
GACATCCAGC CTGCCCCCAG TGCAGAAGAG CTGGCCGAGC AGCTGGACCT GGGTGAGGAG 
GCATCAGCAC TGCTGCTGGA AGACGGTGAA GCCCCAGCCA GCACCAAGGA GGAGCCCTTG 
GAGGAGGAAG CAACGACCCA GAGCCGGGCT GGAAGGGAAG AAGAGGAGAA GGAGCAGGAG 
GAGGAGGGAG ATGTGTCTAC CCTGGAGCAG GGAGAGGGCA GGCTGCAGCT GCGGGCCTCG 
GTGAAGAGAA AAAGCAGGCC CTGCTCCTTG CCTGTGTCCG AGCTGGAGAC GGTGATCGCG 
TCAGCCTGCG GGGACCCCGA GACCCCGCGG ACACACTACA TCCGCATCCA CACCCTGCTG 
CACAGCATGC CCTCCGCCCA GGGCGGCAGC GCGGCAGAGG AGGAGGACGG CGCGGAGGAG 
GAGTCCACCC TCAAGGACTC CTCGGAGAAG GATGGGCTCA GCGAGGTGGA CACGGTGGCC 
GCTGACCCGT CTGCCCTGGA AGAGGACAGA GAAGAGCCCG AGGGGGCTAC TCCAGGCACG 
GCGCACCCTG GCCACTCCGG GGGCCACTTC CCCAGCCTGG CCAATGGCGC GGCCCAGGAT 
GGCGACACGC ACCCCAGCAC CGGGAGCGAG AGCGACTCCA GCCCCAGGCA AGGCGGGGAC 
CACAGTTGCG AGGGCTGTGA CGCGTCCTGC TGCAGCCCCT CGTGCTACAG CTCCTCGTGC 
TACAGCACGT CCTGCTACAG CAGCTCGTGC TACAGCGCCT CGTGCTACAG CCCCTCCTGC 
TACAACGGCA ACAGGTTCGC CAGCCACACG CGCTTCTCCT CCGTGGACAG CGCCAAGATC 
TCCGAGAGCA CGGTCTTCTC CTCGCAAGAC GACGAGGAGG AGGAGAACAG CGCGTTCGAG 
TCGGTACCCG ACTCCATGCA GAGCCCTGAG CTGGACCCGG AGTCCACGAA CGGCGCTGGG 
CCGTGGCAAG ACGAGCTGGC CGCCCCTAGC GGGCACGTGG AAAGAAGCCC GGAAGGTCTG 
GAATCCCCCG TGGCAGGTCC AAGCAATCGG AGAGAAGGTG AATGTCCTAT ACTCCATAAT 
TCCCAGCCAG TAAGCCAGCT TCCTTCCCTG AGGCCTGAAC ATCATCACTA CCCAACAATC 
GATGAGCCTC TTCCACCAAA CTGGGAAGCT CGAATTGACA GCCACGGGCG GGTCTTTTAT 
GTGGACCACG TGAACCGCAC AACCACCTGG CAGCGTCCGA CGGCAGCAGC CACCCCGGAT 
GGCATGCGGA GATCGGGGTC CATCCAGCAG ATGGAGCAAC TCAACAGGCG GTATCAAAAC 
ATTCAGCGAA CCATTGCAAC AGAGAGGTCC GAAGAAGATT CTGGCAGCCA AAGCTGCGAG 
CAAGCCCCAG CAGGAGGAGG CGGAGGTGGA GGGAGTGACT CAGAAGCCGA ATCTTCCCAG 
TCCAGCTTAG ATCTAAGGAG AGAAGGGTCA CTTTCTCCAG TGAACTCACA AAAAATCACC 
TTGCTGCTGC AGTCCCCAGC GGTCAAGTTC ATCACCAACC CCGAGTTCTT CACTGTGCTA 
CATGCCAATT ATAGTGCCTA CCGAGTCTTC ACCAGTAGCA CCTGCTTAAA GCACATGATT 
CTGAAAGTCC GACGGGATGC TCGCAATTTT GAACGCTACC AGCACAACCG GGACTTGGTG 
AATTTCATCA ACATGTTCGC AGACACTCGG CTGGAACTGC CCCGGGGCTG GGAGATCAAA 
ACGGACCAGC AGGGAAAGTC TTTTTTCGTG GACCACAACA GTCGAGCTAC CACTTTCATT 
GACCCCCGAA TCCCTCTTCA GAACGGTCGT CTTCCCAATC ATCTAACTCA CCGACAGCAC 
CTCCAGAGGC TCCGAAGTTA CAGCGCTGGA GAGGCCTCAG AAGTTTCTAG AAACAGAGGA 
GCCTCTTTAC TGGCCAGGCC AGGACACAGC TTAGTAGCTG CTATTCGAAG CCAACATCAA 
CATGAGTCAT TGCCACTGGC ATATAATGAC AAGATTGTGG CATTTCTTCG CCAGCCAAAC 
ATTTTTGAAA TGCTGCAAGA GCGTCAGCCA AGCTTAGCAA GAAACCACAC ACTCAGGGAG 
AAAATCCATT ACATTCGGAC TGAGGGTAAT CACGGGCTTG AGAAGTTGTC CTGTGATGCG 
GATCTGGTCA TTTTGCTGAG TCTCTTTGAA GAAGAGATTA TGTCCTACGT CCCCCTGCAG 
GCTGCCTTCC ACCCTGGGTA TAGCTTCTCT CCCCGATGTT CACCCTGTTC TTCACCTCAG 
AACTCCCCAG GTTTACAGAG AGCCAGTGCA AGAGCCCCTT CCCCCTACCG AAGAGACTTT 
GAGGCCAAGC TCCGCAATTT CTACAGAAAA CTGGAAGCCA AAGGATTTGG TCAGGGTCCG 
GGGAAAATTA AGCTCATTAT TCGCCGGGAT CATTTGTTGG AGGGAACCTT CAATCAGGTG 
ATGGCCTATT CGCGGAAAGA GCTCCAGCGA AACAAGCTCT ACGTCACCTT TGTTGGAGAG 
GAGGGCCTGG ACTACAGTGG CCCCTCGCGG GAGTTCTTCT TCCTTCTGTC TCAGGAGCTC 
TTCAACCCTT ACTATGGACT CTTTGAGTAC TCGGCAAATG ATACTTACAC GGTGCAGATC 
AGCCCCATGT CCGCATTTGT AGAAAACCAT CTTGAGTGGT TCAGGTTTAG CGGTCGCATC 
CTGGGTCTGG CTCTGATCCA TCAGTACCTT CTTGACGCTT TCTTCACGAG GCCCTTCTAC 
AAGGCACTCC TGAGACTGCC CTGTGATTTG AGTGACCTGG AATATTTGGA TGAGGAATTC 
CACCAGAGTT TGCAGTGGAT GAAGGACAAC AACATCACAG ACATCTTAGA CCTCACTTTC 
ACTGTTAATG AAGAGGTTTT TGGACAGGTC ACGGAAAGGG AGTTGAAGTC TGGAGGAGCC 
AACACACAGG TGACGGAGAA AAACAAGAAG GAGTACATCG AGCGCATGGT GAAGTGGCGG 
GTGGAGCGCG GCGTGGTACA GCAGACCGAG GCGCTGGTGC GCGGCTTCTA CGAGGTTGTA 
GACTCGAGGC TGGTGTCCGT GTTTGATGCC AGGGAGCTGG AGCTGGTGAT AGCTGGCACC 
GCGGAAATCG ACCTAAATGA CTGGCGGAAT AACACTGAGT ACCGGGGAGG TTACCACGAT 
GGGCATCTTG TGATCCGCTG GTTCTGGGCT GCGGTGGAGC GCTTCAATAA TGAGCAGAGG 
CTGAGATTAC TGCAGTTTGT CACGGGAACA TCCAGCGTGC CCTACGAAGG CTTCGCAGCC 
CTCCGTGGGA GCAATGGGCT TCGGCGCTTC TGCATAGAGA AATGGGGGAA AATTACTTCT 
CTCCCCAGGG CACACACATG CTTCAACCGA CTGGATCTTC CACCGTATCC CTCGTACTCC 
ATGTTGTATG AAAAGCTGTT AACAGCAGTA GAGGAAACCA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017011882.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867371.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

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CloneID	OHu97114
Clone ID Related Accession (Same CDS sequence)	XM_017011883.2 , XM_017011883.1 , XM_017011882.1
Accession Version	XM_017011882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTACC CGAAAAGGCC AACCGTTAAA GACCCCGGCA GTGTTGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC ATCAGCAGAC TCACTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCCGCCA TGGCGTCTCC TTCTAGAAAC 
TCCCAGAGCC GACGCCGGTG CAAGGAGCCG CTCCGATACA GCTACAACCC CGACCAGTTC 
CACAACATGG ACCTCAGGGG CGGCCCCCAC GATGGCGTCA CCATTCCCCG CTCCACCAGC 
GACACTGACC TGGTCACCTC GGACAGCCGC TCCACGCTCA TGGTCAGCAG CTCCTACTAT 
TCCATCGGGC ACTCTCAGGA CCTGGTCATC CACTGGGACA TAAAGGAGGA AGTGGACGCT 
GGGGACTGGA TTGGCATGTA CCTCATTGAT GAGGTCTTGT CCGAAAACTT TCTGGACTAT 
AAAAACCGTG GAGTCAATGG TTCTCATCGG GGCCAGATCA TCTGGAAGAT CGATGCCAGC 
TCGTACTTTG TGGAACCTGA AACTAAGATC TGCTTCAAAT ACTACCATGG AGTGAGTGGG 
GCCCTGCGAG CAACCACCCC CAGTGTCACG GTCAAAAACT CGGCAGCTCC TATTTTTAAA 
AGCATTGGTG CTGATGAGAC CGTCCAAGGA CAAGGAAGTC GGAGGCTGAT CAGCTTCTCT 
CTCTCAGATT TCCAAGCCAT GGGGTTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAT 
CTGAAGATTT CCATTCAGCC TGGGAAACAC AGCATCTTCC CCGCCCTCCC TCACCATGGA 
CAGGAGAGGA GATCCAAGAT CATAGGCAAC ACCGTGAACC CCATCTGGCA GGCCGAGCAA 
TTCAGTTTTG TGTCCTTGCC CACTGACGTG CTGGAAATTG AGGTGAAGGA CAAGTTTGCC 
AAGAGCCGCC CCATCATCAA GCGCTTCTTG GGAAAGCTGT CGATGCCCGT TCAAAGACTC 
CTGGAGAGAC ACGCCATAGG GGATAGGGTG GTCAGCTACA CACTTGGCCG CAGGCTTCCA 
ACAGATCATG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCCTC CATCCACCCA 
GCAGATGATG AGGAGATTTC CCTGAGTACC GAGCCTGAGT CAGCCCAAAT TCAGGACAGC 
CCCATGAACA ACCTGATGGA AAGCGGCAGT GGGGAACCTC GGTCTGAGGC ACCAGAGTCC 
TCTGAGAGCT GGAAGCCAGA GCAGCTGGGT GAGGGCAGTG TCCCCGATGG TCCAGGGAAC 
CAAAGCATAG AGCTTTCCAG ACCAGCTGAG GAAGCAGCAG TCATCACGGA GGCAGGAGAC 
CAGGGCATGG TCTCTGTGGG ACCTGAAGGG GCTGGGGAGC TCCTGGCCCA GGTGCAAAAG 
GACATCCAGC CTGCCCCCAG TGCAGAAGAG CTGGCCGAGC AGCTGGACCT GGGTGAGGAG 
GCATCAGCAC TGCTGCTGGA AGACGGTGAA GCCCCAGCCA GCACCAAGGA GGAGCCCTTG 
GAGGAGGAAG CAACGACCCA GAGCCGGGCT GGAAGGGAAG AAGAGGAGAA GGAGCAGGAG 
GAGGAGGGAG ATGTGTCTAC CCTGGAGCAG GGAGAGGGCA GGCTGCAGCT GCGGGCCTCG 
GTGAAGAGAA AAAGCAGGCC CTGCTCCTTG CCTGTGTCCG AGCTGGAGAC GGTGATCGCG 
TCAGCCTGCG GGGACCCCGA GACCCCGCGG ACACACTACA TCCGCATCCA CACCCTGCTG 
CACAGCATGC CCTCCGCCCA GGGCGGCAGC GCGGCAGAGG AGGAGGACGG CGCGGAGGAG 
GAGTCCACCC TCAAGGACTC CTCGGAGAAG GATGGGCTCA GCGAGGTGGA CACGGTGGCC 
GCTGACCCGT CTGCCCTGGA AGAGGACAGA GAAGAGCCCG AGGGGGCTAC TCCAGGCACG 
GCGCACCCTG GCCACTCCGG GGGCCACTTC CCCAGCCTGG CCAATGGCGC GGCCCAGGAT 
GGCGACACGC ACCCCAGCAC CGGGAGCGAG AGCGACTCCA GCCCCAGGCA AGGCGGGGAC 
CACAGTTGCG AGGGCTGTGA CGCGTCCTGC TGCAGCCCCT CGTGCTACAG CTCCTCGTGC 
TACAGCACGT CCTGCTACAG CAGCTCGTGC TACAGCGCCT CGTGCTACAG CCCCTCCTGC 
TACAACGGCA ACAGGTTCGC CAGCCACACG CGCTTCTCCT CCGTGGACAG CGCCAAGATC 
TCCGAGAGCA CGGTCTTCTC CTCGCAAGAC GACGAGGAGG AGGAGAACAG CGCGTTCGAG 
TCGGTACCCG ACTCCATGCA GAGCCCTGAG CTGGACCCGG AGTCCACGAA CGGCGCTGGG 
CCGTGGCAAG ACGAGCTGGC CGCCCCTAGC GGGCACGTGG AAAGAAGCCC GGAAGGTCTG 
GAATCCCCCG TGGCAGGTCC AAGCAATCGG AGAGAAGGTG AATGTCCTAT ACTCCATAAT 
TCCCAGCCAG TAAGCCAGCT TCCTTCCCTG AGGCCTGAAC ATCATCACTA CCCAACAATC 
GATGAGCCTC TTCCACCAAA CTGGGAAGCT CGAATTGACA GCCACGGGCG GGTCTTTTAT 
GTGGACCACG TGAACCGCAC AACCACCTGG CAGCGTCCGA CGGCAGCAGC CACCCCGGAT 
GGCATGCGGA GATCGGGGTC CATCCAGCAG ATGGAGCAAC TCAACAGGCG GTATCAAAAC 
ATTCAGCGAA CCATTGCAAC AGAGAGGTCC GAAGAAGATT CTGGCAGCCA AAGCTGCGAG 
CAAGCCCCAG CAGGAGGAGG CGGAGGTGGA GGGAGTGACT CAGAAGCCGA ATCTTCCCAG 
TCCAGCTTAG ATCTAAGGAG AGAAGGGTCA CTTTCTCCAG TGAACTCACA AAAAATCACC 
TTGCTGCTGC AGTCCCCAGC GGTCAAGTTC ATCACCAACC CCGAGTTCTT CACTGTGCTA 
CATGCCAATT ATAGTGCCTA CCGAGTCTTC ACCAGTAGCA CCTGCTTAAA GCACATGATT 
CTGAAAGTCC GACGGGATGC TCGCAATTTT GAACGCTACC AGCACAACCG GGACTTGGTG 
AATTTCATCA ACATGTTCGC AGACACTCGG CTGGAACTGC CCCGGGGCTG GGAGATCAAA 
ACGGACCAGC AGGGAAAGTC TTTTTTCGTG GACCACAACA GTCGAGCTAC CACTTTCATT 
GACCCCCGAA TCCCTCTTCA GAACGGTCGT CTTCCCAATC ATCTAACTCA CCGACAGCAC 
CTCCAGAGGC TCCGAAGTTA CAGCGCTGGA GAGGCCTCAG AAGTTTCTAG AAACAGAGGA 
GCCTCTTTAC TGGCCAGGCC AGGACACAGC TTAGTAGCTG CTATTCGAAG CCAACATCAA 
CATGAGTCAT TGCCACTGGC ATATAATGAC AAGATTGTGG CATTTCTTCG CCAGCCAAAC 
ATTTTTGAAA TGCTGCAAGA GCGTCAGCCA AGCTTAGCAA GAAACCACAC ACTCAGGGAG 
AAAATCCATT ACATTCGGAC TGAGGGTAAT CACGGGCTTG AGAAGTTGTC CTGTGATGCG 
GATCTGGTCA TTTTGCTGAG TCTCTTTGAA GAAGAGATTA TGTCCTACGT CCCCCTGCAG 
GCTGCCTTCC ACCCTGGGTA TAGCTTCTCT CCCCGATGTT CACCCTGTTC TTCACCTCAG 
AACTCCCCAG GTTTACAGAG AGCCAGTGCA AGAGCCCCTT CCCCCTACCG AAGAGACTTT 
GAGGCCAAGC TCCGCAATTT CTACAGAAAA CTGGAAGCCA AAGGATTTGG TCAGGGTCCG 
GGGAAAATTA AGCTCATTAT TCGCCGGGAT CATTTGTTGG AGGGAACCTT CAATCAGGTG 
ATGGCCTATT CGCGGAAAGA GCTCCAGCGA AACAAGCTCT ACGTCACCTT TGTTGGAGAG 
GAGGGCCTGG ACTACAGTGG CCCCTCGCGG GAGTTCTTCT TCCTTCTGTC TCAGGAGCTC 
TTCAACCCTT ACTATGGACT CTTTGAGTAC TCGGCAAATG ATACTTACAC GGTGCAGATC 
AGCCCCATGT CCGCATTTGT AGAAAACCAT CTTGAGTGGT TCAGGTTTAG CGGTCGCATC 
CTGGGTCTGG CTCTGATCCA TCAGTACCTT CTTGACGCTT TCTTCACGAG GCCCTTCTAC 
AAGGCACTCC TGAGACTGCC CTGTGATTTG AGTGACCTGG AATATTTGGA TGAGGAATTC 
CACCAGAGTT TGCAGTGGAT GAAGGACAAC AACATCACAG ACATCTTAGA CCTCACTTTC 
ACTGTTAATG AAGAGGTTTT TGGACAGGTC ACGGAAAGGG AGTTGAAGTC TGGAGGAGCC 
AACACACAGG TGACGGAGAA AAACAAGAAG GAGTACATCG AGCGCATGGT GAAGTGGCGG 
GTGGAGCGCG GCGTGGTACA GCAGACCGAG GCGCTGGTGC GCGGCTTCTA CGAGGTTGTA 
GACTCGAGGC TGGTGTCCGT GTTTGATGCC AGGGAGCTGG AGCTGGTGAT AGCTGGCACC 
GCGGAAATCG ACCTAAATGA CTGGCGGAAT AACACTGAGT ACCGGGGAGG TTACCACGAT 
GGGCATCTTG TGATCCGCTG GTTCTGGGCT GCGGTGGAGC GCTTCAATAA TGAGCAGAGG 
CTGAGATTAC TGCAGTTTGT CACGGGAACA TCCAGCGTGC CCTACGAAGG CTTCGCAGCC 
CTCCGTGGGA GCAATGGGCT TCGGCGCTTC TGCATAGAGA AATGGGGGAA AATTACTTCT 
CTCCCCAGGG CACACACATG CTTCAACCGA CTGGATCTTC CACCGTATCC CTCGTACTCC 
ATGTTGTATG AAAAGCTGTT AACAGCAGTA GAGGAAACCA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867371.1
CDS	529..5448
Translation	MPYPKRPTVKDPGSVVVQGVSKQVARSAFLISRLTPAVAITNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017011882.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011882.1

ORF Insert Sequence:

ATGCCCTACC CGAAAAGGCC AACCGTTAAA GACCCCGGCA GTGTTGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC ATCAGCAGAC TCACTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCCGCCA TGGCGTCTCC TTCTAGAAAC 
TCCCAGAGCC GACGCCGGTG CAAGGAGCCG CTCCGATACA GCTACAACCC CGACCAGTTC 
CACAACATGG ACCTCAGGGG CGGCCCCCAC GATGGCGTCA CCATTCCCCG CTCCACCAGC 
GACACTGACC TGGTCACCTC GGACAGCCGC TCCACGCTCA TGGTCAGCAG CTCCTACTAT 
TCCATCGGGC ACTCTCAGGA CCTGGTCATC CACTGGGACA TAAAGGAGGA AGTGGACGCT 
GGGGACTGGA TTGGCATGTA CCTCATTGAT GAGGTCTTGT CCGAAAACTT TCTGGACTAT 
AAAAACCGTG GAGTCAATGG TTCTCATCGG GGCCAGATCA TCTGGAAGAT CGATGCCAGC 
TCGTACTTTG TGGAACCTGA AACTAAGATC TGCTTCAAAT ACTACCATGG AGTGAGTGGG 
GCCCTGCGAG CAACCACCCC CAGTGTCACG GTCAAAAACT CGGCAGCTCC TATTTTTAAA 
AGCATTGGTG CTGATGAGAC CGTCCAAGGA CAAGGAAGTC GGAGGCTGAT CAGCTTCTCT 
CTCTCAGATT TCCAAGCCAT GGGGTTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAT 
CTGAAGATTT CCATTCAGCC TGGGAAACAC AGCATCTTCC CCGCCCTCCC TCACCATGGA 
CAGGAGAGGA GATCCAAGAT CATAGGCAAC ACCGTGAACC CCATCTGGCA GGCCGAGCAA 
TTCAGTTTTG TGTCCTTGCC CACTGACGTG CTGGAAATTG AGGTGAAGGA CAAGTTTGCC 
AAGAGCCGCC CCATCATCAA GCGCTTCTTG GGAAAGCTGT CGATGCCCGT TCAAAGACTC 
CTGGAGAGAC ACGCCATAGG GGATAGGGTG GTCAGCTACA CACTTGGCCG CAGGCTTCCA 
ACAGATCATG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCCTC CATCCACCCA 
GCAGATGATG AGGAGATTTC CCTGAGTACC GAGCCTGAGT CAGCCCAAAT TCAGGACAGC 
CCCATGAACA ACCTGATGGA AAGCGGCAGT GGGGAACCTC GGTCTGAGGC ACCAGAGTCC 
TCTGAGAGCT GGAAGCCAGA GCAGCTGGGT GAGGGCAGTG TCCCCGATGG TCCAGGGAAC 
CAAAGCATAG AGCTTTCCAG ACCAGCTGAG GAAGCAGCAG TCATCACGGA GGCAGGAGAC 
CAGGGCATGG TCTCTGTGGG ACCTGAAGGG GCTGGGGAGC TCCTGGCCCA GGTGCAAAAG 
GACATCCAGC CTGCCCCCAG TGCAGAAGAG CTGGCCGAGC AGCTGGACCT GGGTGAGGAG 
GCATCAGCAC TGCTGCTGGA AGACGGTGAA GCCCCAGCCA GCACCAAGGA GGAGCCCTTG 
GAGGAGGAAG CAACGACCCA GAGCCGGGCT GGAAGGGAAG AAGAGGAGAA GGAGCAGGAG 
GAGGAGGGAG ATGTGTCTAC CCTGGAGCAG GGAGAGGGCA GGCTGCAGCT GCGGGCCTCG 
GTGAAGAGAA AAAGCAGGCC CTGCTCCTTG CCTGTGTCCG AGCTGGAGAC GGTGATCGCG 
TCAGCCTGCG GGGACCCCGA GACCCCGCGG ACACACTACA TCCGCATCCA CACCCTGCTG 
CACAGCATGC CCTCCGCCCA GGGCGGCAGC GCGGCAGAGG AGGAGGACGG CGCGGAGGAG 
GAGTCCACCC TCAAGGACTC CTCGGAGAAG GATGGGCTCA GCGAGGTGGA CACGGTGGCC 
GCTGACCCGT CTGCCCTGGA AGAGGACAGA GAAGAGCCCG AGGGGGCTAC TCCAGGCACG 
GCGCACCCTG GCCACTCCGG GGGCCACTTC CCCAGCCTGG CCAATGGCGC GGCCCAGGAT 
GGCGACACGC ACCCCAGCAC CGGGAGCGAG AGCGACTCCA GCCCCAGGCA AGGCGGGGAC 
CACAGTTGCG AGGGCTGTGA CGCGTCCTGC TGCAGCCCCT CGTGCTACAG CTCCTCGTGC 
TACAGCACGT CCTGCTACAG CAGCTCGTGC TACAGCGCCT CGTGCTACAG CCCCTCCTGC 
TACAACGGCA ACAGGTTCGC CAGCCACACG CGCTTCTCCT CCGTGGACAG CGCCAAGATC 
TCCGAGAGCA CGGTCTTCTC CTCGCAAGAC GACGAGGAGG AGGAGAACAG CGCGTTCGAG 
TCGGTACCCG ACTCCATGCA GAGCCCTGAG CTGGACCCGG AGTCCACGAA CGGCGCTGGG 
CCGTGGCAAG ACGAGCTGGC CGCCCCTAGC GGGCACGTGG AAAGAAGCCC GGAAGGTCTG 
GAATCCCCCG TGGCAGGTCC AAGCAATCGG AGAGAAGGTG AATGTCCTAT ACTCCATAAT 
TCCCAGCCAG TAAGCCAGCT TCCTTCCCTG AGGCCTGAAC ATCATCACTA CCCAACAATC 
GATGAGCCTC TTCCACCAAA CTGGGAAGCT CGAATTGACA GCCACGGGCG GGTCTTTTAT 
GTGGACCACG TGAACCGCAC AACCACCTGG CAGCGTCCGA CGGCAGCAGC CACCCCGGAT 
GGCATGCGGA GATCGGGGTC CATCCAGCAG ATGGAGCAAC TCAACAGGCG GTATCAAAAC 
ATTCAGCGAA CCATTGCAAC AGAGAGGTCC GAAGAAGATT CTGGCAGCCA AAGCTGCGAG 
CAAGCCCCAG CAGGAGGAGG CGGAGGTGGA GGGAGTGACT CAGAAGCCGA ATCTTCCCAG 
TCCAGCTTAG ATCTAAGGAG AGAAGGGTCA CTTTCTCCAG TGAACTCACA AAAAATCACC 
TTGCTGCTGC AGTCCCCAGC GGTCAAGTTC ATCACCAACC CCGAGTTCTT CACTGTGCTA 
CATGCCAATT ATAGTGCCTA CCGAGTCTTC ACCAGTAGCA CCTGCTTAAA GCACATGATT 
CTGAAAGTCC GACGGGATGC TCGCAATTTT GAACGCTACC AGCACAACCG GGACTTGGTG 
AATTTCATCA ACATGTTCGC AGACACTCGG CTGGAACTGC CCCGGGGCTG GGAGATCAAA 
ACGGACCAGC AGGGAAAGTC TTTTTTCGTG GACCACAACA GTCGAGCTAC CACTTTCATT 
GACCCCCGAA TCCCTCTTCA GAACGGTCGT CTTCCCAATC ATCTAACTCA CCGACAGCAC 
CTCCAGAGGC TCCGAAGTTA CAGCGCTGGA GAGGCCTCAG AAGTTTCTAG AAACAGAGGA 
GCCTCTTTAC TGGCCAGGCC AGGACACAGC TTAGTAGCTG CTATTCGAAG CCAACATCAA 
CATGAGTCAT TGCCACTGGC ATATAATGAC AAGATTGTGG CATTTCTTCG CCAGCCAAAC 
ATTTTTGAAA TGCTGCAAGA GCGTCAGCCA AGCTTAGCAA GAAACCACAC ACTCAGGGAG 
AAAATCCATT ACATTCGGAC TGAGGGTAAT CACGGGCTTG AGAAGTTGTC CTGTGATGCG 
GATCTGGTCA TTTTGCTGAG TCTCTTTGAA GAAGAGATTA TGTCCTACGT CCCCCTGCAG 
GCTGCCTTCC ACCCTGGGTA TAGCTTCTCT CCCCGATGTT CACCCTGTTC TTCACCTCAG 
AACTCCCCAG GTTTACAGAG AGCCAGTGCA AGAGCCCCTT CCCCCTACCG AAGAGACTTT 
GAGGCCAAGC TCCGCAATTT CTACAGAAAA CTGGAAGCCA AAGGATTTGG TCAGGGTCCG 
GGGAAAATTA AGCTCATTAT TCGCCGGGAT CATTTGTTGG AGGGAACCTT CAATCAGGTG 
ATGGCCTATT CGCGGAAAGA GCTCCAGCGA AACAAGCTCT ACGTCACCTT TGTTGGAGAG 
GAGGGCCTGG ACTACAGTGG CCCCTCGCGG GAGTTCTTCT TCCTTCTGTC TCAGGAGCTC 
TTCAACCCTT ACTATGGACT CTTTGAGTAC TCGGCAAATG ATACTTACAC GGTGCAGATC 
AGCCCCATGT CCGCATTTGT AGAAAACCAT CTTGAGTGGT TCAGGTTTAG CGGTCGCATC 
CTGGGTCTGG CTCTGATCCA TCAGTACCTT CTTGACGCTT TCTTCACGAG GCCCTTCTAC 
AAGGCACTCC TGAGACTGCC CTGTGATTTG AGTGACCTGG AATATTTGGA TGAGGAATTC 
CACCAGAGTT TGCAGTGGAT GAAGGACAAC AACATCACAG ACATCTTAGA CCTCACTTTC 
ACTGTTAATG AAGAGGTTTT TGGACAGGTC ACGGAAAGGG AGTTGAAGTC TGGAGGAGCC 
AACACACAGG TGACGGAGAA AAACAAGAAG GAGTACATCG AGCGCATGGT GAAGTGGCGG 
GTGGAGCGCG GCGTGGTACA GCAGACCGAG GCGCTGGTGC GCGGCTTCTA CGAGGTTGTA 
GACTCGAGGC TGGTGTCCGT GTTTGATGCC AGGGAGCTGG AGCTGGTGAT AGCTGGCACC 
GCGGAAATCG ACCTAAATGA CTGGCGGAAT AACACTGAGT ACCGGGGAGG TTACCACGAT 
GGGCATCTTG TGATCCGCTG GTTCTGGGCT GCGGTGGAGC GCTTCAATAA TGAGCAGAGG 
CTGAGATTAC TGCAGTTTGT CACGGGAACA TCCAGCGTGC CCTACGAAGG CTTCGCAGCC 
CTCCGTGGGA GCAATGGGCT TCGGCGCTTC TGCATAGAGA AATGGGGGAA AATTACTTCT 
CTCCCCAGGG CACACACATG CTTCAACCGA CTGGATCTTC CACCGTATCC CTCGTACTCC 
ATGTTGTATG AAAAGCTGTT AACAGCAGTA GAGGAAACCA GCACCTTTGG ACTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017011884.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867373.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

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CloneID	OHu97115
Clone ID Related Accession (Same CDS sequence)	XM_017011884.1
Accession Version	XM_017011884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTACC CGAAAAGGCC AACCGTTAAA GACCCCGGCA GTGTTGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC ATCAGCAGAC TCACTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCCGCCA TGGCGTCTCC TTCTAGAAAC 
TCCCAGAGCC GACGCCGGTG CAAGGAGCCG CTCCGATACA GCTACAACCC CGACCAGTTC 
CACAACATGG ACCTCAGGGG CGGCCCCCAC GATGGCGTCA CCATTCCCCG CTCCACCAGC 
GACACTGACC TGGTCACCTC GGACAGCCGC TCCACGCTCA TGGTCAGCAG CTCCTACTAT 
TCCATCGGGC ACTCTCAGGA CCTGGTCATC CACTGGGACA TAAAGGAGGA AGTGGACGCT 
GGGGACTGGA TTGGCATGTA CCTCATTGAT GAGGTCTTGT CCGAAAACTT TCTGGACTAT 
AAAAACCGTG GAGTCAATGG TTCTCATCGG GGCCAGATCA TCTGGAAGAT CGATGCCAGC 
TCGTACTTTG TGGAACCTGA AACTAAGATC TGCTTCAAAT ACTACCATGG AGTGAGTGGG 
GCCCTGCGAG CAACCACCCC CAGTGTCACG GTCAAAAACT CGGCAGCTCC TATTTTTAAA 
AGCATTGGTG CTGATGAGAC CGTCCAAGGA CAAGGAAGTC GGAGGCTGAT CAGCTTCTCT 
CTCTCAGATT TCCAAGCCAT GGGGTTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAT 
CTGAAGATTT CCATTCAGCC TGGGAAACAC AGCATCTTCC CCGCCCTCCC TCACCATGGA 
CAGGAGAGGA GATCCAAGAT CATAGGCAAC ACCGTGAACC CCATCTGGCA GGCCGAGCAA 
TTCAGTTTTG TGTCCTTGCC CACTGACGTG CTGGAAATTG AGGTGAAGGA CAAGTTTGCC 
AAGAGCCGCC CCATCATCAA GCGCTTCTTG GGAAAGCTGT CGATGCCCGT TCAAAGACTC 
CTGGAGAGAC ACGCCATAGG GGATAGGGTG GTCAGCTACA CACTTGGCCG CAGGCTTCCA 
ACAGATCATG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCCTC CATCCACCCA 
GATGATGAGG AGATTTCCCT GAGTACCGAG CCTGAGTCAG CCCAAATTCA GGACAGCCCC 
ATGAACAACC TGATGGAAAG CGGCAGTGGG GAACCTCGGT CTGAGGCACC AGAGTCCTCT 
GAGAGCTGGA AGCCAGAGCA GCTGGGTGAG GGCAGTGTCC CCGATGGTCC AGGGAACCAA 
AGCATAGAGC TTTCCAGACC AGCTGAGGAA GCAGCAGTCA TCACGGAGGC AGGAGACCAG 
GGCATGGTCT CTGTGGGACC TGAAGGGGCT GGGGAGCTCC TGGCCCAGGT GCAAAAGGAC 
ATCCAGCCTG CCCCCAGTGC AGAAGAGCTG GCCGAGCAGC TGGACCTGGG TGAGGAGGCA 
TCAGCACTGC TGCTGGAAGA CGGTGAAGCC CCAGCCAGCA CCAAGGAGGA GCCCTTGGAG 
GAGGAAGCAA CGACCCAGAG CCGGGCTGGA AGGGAAGAAG AGGAGAAGGA GCAGGAGGAG 
GAGGGAGATG TGTCTACCCT GGAGCAGGGA GAGGGCAGGC TGCAGCTGCG GGCCTCGGTG 
AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT GTGTCCGAGC TGGAGACGGT GATCGCGTCA 
GCCTGCGGGG ACCCCGAGAC CCCGCGGACA CACTACATCC GCATCCACAC CCTGCTGCAC 
AGCATGCCCT CCGCCCAGGG CGGCAGCGCG GCAGAGGAGG AGGACGGCGC GGAGGAGGAG 
TCCACCCTCA AGGACTCCTC GGAGAAGGAT GGGCTCAGCG AGGTGGACAC GGTGGCCGCT 
GACCCGTCTG CCCTGGAAGA GGACAGAGAA GAGCCCGAGG GGGCTACTCC AGGCACGGCG 
CACCCTGGCC ACTCCGGGGG CCACTTCCCC AGCCTGGCCA ATGGCGCGGC CCAGGATGGC 
GACACGCACC CCAGCACCGG GAGCGAGAGC GACTCCAGCC CCAGGCAAGG CGGGGACCAC 
AGTTGCGAGG GCTGTGACGC GTCCTGCTGC AGCCCCTCGT GCTACAGCTC CTCGTGCTAC 
AGCACGTCCT GCTACAGCAG CTCGTGCTAC AGCGCCTCGT GCTACAGCCC CTCCTGCTAC 
AACGGCAACA GGTTCGCCAG CCACACGCGC TTCTCCTCCG TGGACAGCGC CAAGATCTCC 
GAGAGCACGG TCTTCTCCTC GCAAGACGAC GAGGAGGAGG AGAACAGCGC GTTCGAGTCG 
GTACCCGACT CCATGCAGAG CCCTGAGCTG GACCCGGAGT CCACGAACGG CGCTGGGCCG 
TGGCAAGACG AGCTGGCCGC CCCTAGCGGG CACGTGGAAA GAAGCCCGGA AGGTCTGGAA 
TCCCCCGTGG CAGGTCCAAG CAATCGGAGA GAAGGTGAAT GTCCTATACT CCATAATTCC 
CAGCCAGTAA GCCAGCTTCC TTCCCTGAGG CCTGAACATC ATCACTACCC AACAATCGAT 
GAGCCTCTTC CACCAAACTG GGAAGCTCGA ATTGACAGCC ACGGGCGGGT CTTTTATGTG 
GACCACGTGA ACCGCACAAC CACCTGGCAG CGTCCGACGG CAGCAGCCAC CCCGGATGGC 
ATGCGGAGAT CGGGGTCCAT CCAGCAGATG GAGCAACTCA ACAGGCGGTA TCAAAACATT 
CAGCGAACCA TTGCAACAGA GAGGTCCGAA GAAGATTCTG GCAGCCAAAG CTGCGAGCAA 
GCCCCAGCAG GAGGAGGCGG AGGTGGAGGG AGTGACTCAG AAGCCGAATC TTCCCAGTCC 
AGCTTAGATC TAAGGAGAGA AGGGTCACTT TCTCCAGTGA ACTCACAAAA AATCACCTTG 
CTGCTGCAGT CCCCAGCGGT CAAGTTCATC ACCAACCCCG AGTTCTTCAC TGTGCTACAT 
GCCAATTATA GTGCCTACCG AGTCTTCACC AGTAGCACCT GCTTAAAGCA CATGATTCTG 
AAAGTCCGAC GGGATGCTCG CAATTTTGAA CGCTACCAGC ACAACCGGGA CTTGGTGAAT 
TTCATCAACA TGTTCGCAGA CACTCGGCTG GAACTGCCCC GGGGCTGGGA GATCAAAACG 
GACCAGCAGG GAAAGTCTTT TTTCGTGGAC CACAACAGTC GAGCTACCAC TTTCATTGAC 
CCCCGAATCC CTCTTCAGAA CGGTCGTCTT CCCAATCATC TAACTCACCG ACAGCACCTC 
CAGAGGCTCC GAAGTTACAG CGCTGGAGAG GCCTCAGAAG TTTCTAGAAA CAGAGGAGCC 
TCTTTACTGG CCAGGCCAGG ACACAGCTTA GTAGCTGCTA TTCGAAGCCA ACATCAACAT 
GAGTCATTGC CACTGGCATA TAATGACAAG ATTGTGGCAT TTCTTCGCCA GCCAAACATT 
TTTGAAATGC TGCAAGAGCG TCAGCCAAGC TTAGCAAGAA ACCACACACT CAGGGAGAAA 
ATCCATTACA TTCGGACTGA GGGTAATCAC GGGCTTGAGA AGTTGTCCTG TGATGCGGAT 
CTGGTCATTT TGCTGAGTCT CTTTGAAGAA GAGATTATGT CCTACGTCCC CCTGCAGGCT 
GCCTTCCACC CTGGGTATAG CTTCTCTCCC CGATGTTCAC CCTGTTCTTC ACCTCAGAAC 
TCCCCAGGTT TACAGAGAGC CAGTGCAAGA GCCCCTTCCC CCTACCGAAG AGACTTTGAG 
GCCAAGCTCC GCAATTTCTA CAGAAAACTG GAAGCCAAAG GATTTGGTCA GGGTCCGGGG 
AAAATTAAGC TCATTATTCG CCGGGATCAT TTGTTGGAGG GAACCTTCAA TCAGGTGATG 
GCCTATTCGC GGAAAGAGCT CCAGCGAAAC AAGCTCTACG TCACCTTTGT TGGAGAGGAG 
GGCCTGGACT ACAGTGGCCC CTCGCGGGAG TTCTTCTTCC TTCTGTCTCA GGAGCTCTTC 
AACCCTTACT ATGGACTCTT TGAGTACTCG GCAAATGATA CTTACACGGT GCAGATCAGC 
CCCATGTCCG CATTTGTAGA AAACCATCTT GAGTGGTTCA GGTTTAGCGG TCGCATCCTG 
GGTCTGGCTC TGATCCATCA GTACCTTCTT GACGCTTTCT TCACGAGGCC CTTCTACAAG 
GCACTCCTGA GACTGCCCTG TGATTTGAGT GACCTGGAAT ATTTGGATGA GGAATTCCAC 
CAGAGTTTGC AGTGGATGAA GGACAACAAC ATCACAGACA TCTTAGACCT CACTTTCACT 
GTTAATGAAG AGGTTTTTGG ACAGGTCACG GAAAGGGAGT TGAAGTCTGG AGGAGCCAAC 
ACACAGGTGA CGGAGAAAAA CAAGAAGGAG TACATCGAGC GCATGGTGAA GTGGCGGGTG 
GAGCGCGGCG TGGTACAGCA GACCGAGGCG CTGGTGCGCG GCTTCTACGA GGTTGTAGAC 
TCGAGGCTGG TGTCCGTGTT TGATGCCAGG GAGCTGGAGC TGGTGATAGC TGGCACCGCG 
GAAATCGACC TAAATGACTG GCGGAATAAC ACTGAGTACC GGGGAGGTTA CCACGATGGG 
CATCTTGTGA TCCGCTGGTT CTGGGCTGCG GTGGAGCGCT TCAATAATGA GCAGAGGCTG 
AGATTACTGC AGTTTGTCAC GGGAACATCC AGCGTGCCCT ACGAAGGCTT CGCAGCCCTC 
CGTGGGAGCA ATGGGCTTCG GCGCTTCTGC ATAGAGAAAT GGGGGAAAAT TACTTCTCTC 
CCCAGGGCAC ACACATGCTT CAACCGACTG GATCTTCCAC CGTATCCCTC GTACTCCATG 
TTGTATGAAA AGCTGTTAAC AGCAGTAGAG GAAACCAGCA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867373.1
CDS	529..5445
Translation	MPYPKRPTVKDPGSVVVQGVSKQVARSAFLISRLTPAVAITNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017011884.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011884.1

ORF Insert Sequence:

ATGCCCTACC CGAAAAGGCC AACCGTTAAA GACCCCGGCA GTGTTGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC ATCAGCAGAC TCACTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCCGCCA TGGCGTCTCC TTCTAGAAAC 
TCCCAGAGCC GACGCCGGTG CAAGGAGCCG CTCCGATACA GCTACAACCC CGACCAGTTC 
CACAACATGG ACCTCAGGGG CGGCCCCCAC GATGGCGTCA CCATTCCCCG CTCCACCAGC 
GACACTGACC TGGTCACCTC GGACAGCCGC TCCACGCTCA TGGTCAGCAG CTCCTACTAT 
TCCATCGGGC ACTCTCAGGA CCTGGTCATC CACTGGGACA TAAAGGAGGA AGTGGACGCT 
GGGGACTGGA TTGGCATGTA CCTCATTGAT GAGGTCTTGT CCGAAAACTT TCTGGACTAT 
AAAAACCGTG GAGTCAATGG TTCTCATCGG GGCCAGATCA TCTGGAAGAT CGATGCCAGC 
TCGTACTTTG TGGAACCTGA AACTAAGATC TGCTTCAAAT ACTACCATGG AGTGAGTGGG 
GCCCTGCGAG CAACCACCCC CAGTGTCACG GTCAAAAACT CGGCAGCTCC TATTTTTAAA 
AGCATTGGTG CTGATGAGAC CGTCCAAGGA CAAGGAAGTC GGAGGCTGAT CAGCTTCTCT 
CTCTCAGATT TCCAAGCCAT GGGGTTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAT 
CTGAAGATTT CCATTCAGCC TGGGAAACAC AGCATCTTCC CCGCCCTCCC TCACCATGGA 
CAGGAGAGGA GATCCAAGAT CATAGGCAAC ACCGTGAACC CCATCTGGCA GGCCGAGCAA 
TTCAGTTTTG TGTCCTTGCC CACTGACGTG CTGGAAATTG AGGTGAAGGA CAAGTTTGCC 
AAGAGCCGCC CCATCATCAA GCGCTTCTTG GGAAAGCTGT CGATGCCCGT TCAAAGACTC 
CTGGAGAGAC ACGCCATAGG GGATAGGGTG GTCAGCTACA CACTTGGCCG CAGGCTTCCA 
ACAGATCATG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCCTC CATCCACCCA 
GATGATGAGG AGATTTCCCT GAGTACCGAG CCTGAGTCAG CCCAAATTCA GGACAGCCCC 
ATGAACAACC TGATGGAAAG CGGCAGTGGG GAACCTCGGT CTGAGGCACC AGAGTCCTCT 
GAGAGCTGGA AGCCAGAGCA GCTGGGTGAG GGCAGTGTCC CCGATGGTCC AGGGAACCAA 
AGCATAGAGC TTTCCAGACC AGCTGAGGAA GCAGCAGTCA TCACGGAGGC AGGAGACCAG 
GGCATGGTCT CTGTGGGACC TGAAGGGGCT GGGGAGCTCC TGGCCCAGGT GCAAAAGGAC 
ATCCAGCCTG CCCCCAGTGC AGAAGAGCTG GCCGAGCAGC TGGACCTGGG TGAGGAGGCA 
TCAGCACTGC TGCTGGAAGA CGGTGAAGCC CCAGCCAGCA CCAAGGAGGA GCCCTTGGAG 
GAGGAAGCAA CGACCCAGAG CCGGGCTGGA AGGGAAGAAG AGGAGAAGGA GCAGGAGGAG 
GAGGGAGATG TGTCTACCCT GGAGCAGGGA GAGGGCAGGC TGCAGCTGCG GGCCTCGGTG 
AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT GTGTCCGAGC TGGAGACGGT GATCGCGTCA 
GCCTGCGGGG ACCCCGAGAC CCCGCGGACA CACTACATCC GCATCCACAC CCTGCTGCAC 
AGCATGCCCT CCGCCCAGGG CGGCAGCGCG GCAGAGGAGG AGGACGGCGC GGAGGAGGAG 
TCCACCCTCA AGGACTCCTC GGAGAAGGAT GGGCTCAGCG AGGTGGACAC GGTGGCCGCT 
GACCCGTCTG CCCTGGAAGA GGACAGAGAA GAGCCCGAGG GGGCTACTCC AGGCACGGCG 
CACCCTGGCC ACTCCGGGGG CCACTTCCCC AGCCTGGCCA ATGGCGCGGC CCAGGATGGC 
GACACGCACC CCAGCACCGG GAGCGAGAGC GACTCCAGCC CCAGGCAAGG CGGGGACCAC 
AGTTGCGAGG GCTGTGACGC GTCCTGCTGC AGCCCCTCGT GCTACAGCTC CTCGTGCTAC 
AGCACGTCCT GCTACAGCAG CTCGTGCTAC AGCGCCTCGT GCTACAGCCC CTCCTGCTAC 
AACGGCAACA GGTTCGCCAG CCACACGCGC TTCTCCTCCG TGGACAGCGC CAAGATCTCC 
GAGAGCACGG TCTTCTCCTC GCAAGACGAC GAGGAGGAGG AGAACAGCGC GTTCGAGTCG 
GTACCCGACT CCATGCAGAG CCCTGAGCTG GACCCGGAGT CCACGAACGG CGCTGGGCCG 
TGGCAAGACG AGCTGGCCGC CCCTAGCGGG CACGTGGAAA GAAGCCCGGA AGGTCTGGAA 
TCCCCCGTGG CAGGTCCAAG CAATCGGAGA GAAGGTGAAT GTCCTATACT CCATAATTCC 
CAGCCAGTAA GCCAGCTTCC TTCCCTGAGG CCTGAACATC ATCACTACCC AACAATCGAT 
GAGCCTCTTC CACCAAACTG GGAAGCTCGA ATTGACAGCC ACGGGCGGGT CTTTTATGTG 
GACCACGTGA ACCGCACAAC CACCTGGCAG CGTCCGACGG CAGCAGCCAC CCCGGATGGC 
ATGCGGAGAT CGGGGTCCAT CCAGCAGATG GAGCAACTCA ACAGGCGGTA TCAAAACATT 
CAGCGAACCA TTGCAACAGA GAGGTCCGAA GAAGATTCTG GCAGCCAAAG CTGCGAGCAA 
GCCCCAGCAG GAGGAGGCGG AGGTGGAGGG AGTGACTCAG AAGCCGAATC TTCCCAGTCC 
AGCTTAGATC TAAGGAGAGA AGGGTCACTT TCTCCAGTGA ACTCACAAAA AATCACCTTG 
CTGCTGCAGT CCCCAGCGGT CAAGTTCATC ACCAACCCCG AGTTCTTCAC TGTGCTACAT 
GCCAATTATA GTGCCTACCG AGTCTTCACC AGTAGCACCT GCTTAAAGCA CATGATTCTG 
AAAGTCCGAC GGGATGCTCG CAATTTTGAA CGCTACCAGC ACAACCGGGA CTTGGTGAAT 
TTCATCAACA TGTTCGCAGA CACTCGGCTG GAACTGCCCC GGGGCTGGGA GATCAAAACG 
GACCAGCAGG GAAAGTCTTT TTTCGTGGAC CACAACAGTC GAGCTACCAC TTTCATTGAC 
CCCCGAATCC CTCTTCAGAA CGGTCGTCTT CCCAATCATC TAACTCACCG ACAGCACCTC 
CAGAGGCTCC GAAGTTACAG CGCTGGAGAG GCCTCAGAAG TTTCTAGAAA CAGAGGAGCC 
TCTTTACTGG CCAGGCCAGG ACACAGCTTA GTAGCTGCTA TTCGAAGCCA ACATCAACAT 
GAGTCATTGC CACTGGCATA TAATGACAAG ATTGTGGCAT TTCTTCGCCA GCCAAACATT 
TTTGAAATGC TGCAAGAGCG TCAGCCAAGC TTAGCAAGAA ACCACACACT CAGGGAGAAA 
ATCCATTACA TTCGGACTGA GGGTAATCAC GGGCTTGAGA AGTTGTCCTG TGATGCGGAT 
CTGGTCATTT TGCTGAGTCT CTTTGAAGAA GAGATTATGT CCTACGTCCC CCTGCAGGCT 
GCCTTCCACC CTGGGTATAG CTTCTCTCCC CGATGTTCAC CCTGTTCTTC ACCTCAGAAC 
TCCCCAGGTT TACAGAGAGC CAGTGCAAGA GCCCCTTCCC CCTACCGAAG AGACTTTGAG 
GCCAAGCTCC GCAATTTCTA CAGAAAACTG GAAGCCAAAG GATTTGGTCA GGGTCCGGGG 
AAAATTAAGC TCATTATTCG CCGGGATCAT TTGTTGGAGG GAACCTTCAA TCAGGTGATG 
GCCTATTCGC GGAAAGAGCT CCAGCGAAAC AAGCTCTACG TCACCTTTGT TGGAGAGGAG 
GGCCTGGACT ACAGTGGCCC CTCGCGGGAG TTCTTCTTCC TTCTGTCTCA GGAGCTCTTC 
AACCCTTACT ATGGACTCTT TGAGTACTCG GCAAATGATA CTTACACGGT GCAGATCAGC 
CCCATGTCCG CATTTGTAGA AAACCATCTT GAGTGGTTCA GGTTTAGCGG TCGCATCCTG 
GGTCTGGCTC TGATCCATCA GTACCTTCTT GACGCTTTCT TCACGAGGCC CTTCTACAAG 
GCACTCCTGA GACTGCCCTG TGATTTGAGT GACCTGGAAT ATTTGGATGA GGAATTCCAC 
CAGAGTTTGC AGTGGATGAA GGACAACAAC ATCACAGACA TCTTAGACCT CACTTTCACT 
GTTAATGAAG AGGTTTTTGG ACAGGTCACG GAAAGGGAGT TGAAGTCTGG AGGAGCCAAC 
ACACAGGTGA CGGAGAAAAA CAAGAAGGAG TACATCGAGC GCATGGTGAA GTGGCGGGTG 
GAGCGCGGCG TGGTACAGCA GACCGAGGCG CTGGTGCGCG GCTTCTACGA GGTTGTAGAC 
TCGAGGCTGG TGTCCGTGTT TGATGCCAGG GAGCTGGAGC TGGTGATAGC TGGCACCGCG 
GAAATCGACC TAAATGACTG GCGGAATAAC ACTGAGTACC GGGGAGGTTA CCACGATGGG 
CATCTTGTGA TCCGCTGGTT CTGGGCTGCG GTGGAGCGCT TCAATAATGA GCAGAGGCTG 
AGATTACTGC AGTTTGTCAC GGGAACATCC AGCGTGCCCT ACGAAGGCTT CGCAGCCCTC 
CGTGGGAGCA ATGGGCTTCG GCGCTTCTGC ATAGAGAAAT GGGGGAAAAT TACTTCTCTC 
CCCAGGGCAC ACACATGCTT CAACCGACTG GATCTTCCAC CGTATCCCTC GTACTCCATG 
TTGTATGAAA AGCTGTTAAC AGCAGTAGAG GAAACCAGCA CCTTTGGACT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017011887.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867376.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X10, mRNA.

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CloneID	OHu97116
Clone ID Related Accession (Same CDS sequence)	XM_017011887.1
Accession Version	XM_017011887.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTACC CGAAAAGGCC AACCGTTAAA GACCCCGGCA GTGTTGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC ATCAGCAGAC TCACTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCCGCCA TGGCGTCTCC TTCTAGAAAC 
TCCCAGAGCC GACGCCGGTG CAAGGAGCCG CTCCGATACA GCTACAACCC CGACCAGTTC 
CACAACATGG ACCTCAGGGG CGGCCCCCAC GATGGCGTCA CCATTCCCCG CTCCACCAGC 
GACACTGACC TGGTCACCTC GGACAGCCGC TCCACGCTCA TGGTCAGCAG CTCCTACTAT 
TCCATCGGGC ACTCTCAGGA CCTGGTCATC CACTGGGACA TAAAGGAGGA AGTGGACGCT 
GGGGACTGGA TTGGCATGTA CCTCATTGAT GAGGTCTTGT CCGAAAACTT TCTGGACTAT 
AAAAACCGTG GAGTCAATGG TTCTCATCGG GGCCAGATCA TCTGGAAGAT CGATGCCAGC 
TCGTACTTTG TGGAACCTGA AACTAAGATC TGCTTCAAAT ACTACCATGG AGTGAGTGGG 
GCCCTGCGAG CAACCACCCC CAGTGTCACG GTCAAAAACT CGGCAGCTCC TATTTTTAAA 
AGCATTGGTG CTGATGAGAC CGTCCAAGGA CAAGGAAGTC GGAGGCTGAT CAGCTTCTCT 
CTCTCAGATT TCCAAGCCAT GGGGTTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAT 
CTGAAGATTT CCATTCAGCC TGGGAAACAC AGCATCTTCC CCGCCCTCCC TCACCATGGA 
CAGGAGAGGA GATCCAAGAT CATAGGCAAC ACCGTGAACC CCATCTGGCA GGCCGAGCAA 
TTCAGTTTTG TGTCCTTGCC CACTGACGTG CTGGAAATTG AGGTGAAGGA CAAGTTTGCC 
AAGAGCCGCC CCATCATCAA GCGCTTCTTG GGAAAGCTGT CGATGCCCGT TCAAAGACTC 
CTGGAGAGAC ACGCCATAGG GGATAGGGTG GTCAGCTACA CACTTGGCCG CAGGCTTCCA 
ACAGATCATG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCCTC CATCCACCCA 
GCAGATGATG AGGAGATTTC CCTGAGTACC GAGCCTGAGT CAGCCCAAAT TCAGGACAGC 
CCCATGAACA ACCTGATGGA AAGCGGCAGT GGGGAACCTC GGTCTGAGGC ACCAGAGTCC 
TCTGAGAGCT GGAAGCCAGA GCAGCTGGGT GAGGGCAGTG TCCCCGATGG TCCAGGGAAC 
CAAAGCATAG AGCTTTCCAG ACCAGCTGAG GAAGCAGCAG TCATCACGGA GGCAGGAGAC 
CAGGGCATGG TCTCTGTGGG ACCTGAAGGG GCTGGGGAGC TCCTGGCCCA GGTGCAAAAG 
GACATCCAGC CTGCCCCCAG TGCAGAAGAG CTGGCCGAGC AGCTGGACCT GGGTGAGGAG 
GCATCAGCAC TGCTGCTGGA AGACGGTGAA GCCCCAGCCA GCACCAAGGA GGAGCCCTTG 
GAGGAGGAAG CAACGACCCA GAGCCGGGCT GGAAGGGAAG AAGAGGAGAA GGAGCAGGAG 
GAGGAGGGAG ATGTGTCTAC CCTGGAGCAG GGAGAGGGCA GGCTGCAGCT GCGGGCCTCG 
GTGAAGAGAA AAAGCAGGCC CTGCTCCTTG CCTGTGTCCG AGCTGGAGAC GGTGATCGCG 
TCAGCCTGCG GGGACCCCGA GACCCCGCGG ACACACTACA TCCGCATCCA CACCCTGCTG 
CACAGCATGC CCTCCGCCCA GGGCGGCAGC GCGGCAGAGG AGGAGGACGG CGCGGAGGAG 
GAGTCCACCC TCAAGGACTC CTCGGAGAAG GATGGGCTCA GCGAGGTGGA CACGGTGGCC 
GCTGACCCGT CTGCCCTGGA AGAGGACAGA GAAGAGCCCG AGGGGGCTAC TCCAGGCACG 
GCGCACCCTG GCCACTCCGG GGGCCACTTC CCCAGCCTGG CCAATGGCGC GGCCCAGGAT 
GGCGACACGC ACCCCAGCAC CGGGAGCGAG AGCGACTCCA GCCCCAGGCA AGGCGGGGAC 
CACAGTTGCG AGGGCTGTGA CGCGTCCTGC TGCAGCCCCT CGTGCTACAG CTCCTCGTGC 
TACAGCACGT CCTGCTACAG CAGCTCGTGC TACAGCGCCT CGTGCTACAG CCCCTCCTGC 
TACAACGGCA ACAGGTTCGC CAGCCACACG CGCTTCTCCT CCGTGGACAG CGCCAAGATC 
TCCGAGAGCA CGGTCTTCTC CTCGCAAGAC GACGAGGAGG AGGAGAACAG CGCGTTCGAG 
TCGGTACCCG ACTCCATGCA GAGCCCTGAG CTGGACCCGG AGTCCACGAA CGGCGCTGGG 
CCGTGGCAAG ACGAGCTGGC CGCCCCTAGC GGGCACGTGG AAAGAAGCCC GGAAGGTCTG 
GAATCCCCCG TGGCAGGTCC AAGCAATCGG AGAGAAGACT GGGAAGCTCG AATTGACAGC 
CACGGGCGGG TCTTTTATGT GGACCACGTG AACCGCACAA CCACCTGGCA GCGTCCGACG 
GCAGCAGCCA CCCCGGATGG CATGCGGAGA TCGGGGTCCA TCCAGCAGAT GGAGCAACTC 
AACAGGCGGT ATCAAAACAT TCAGCGAACC ATTGCAACAG AGAGGTCCGA AGAAGATTCT 
GGCAGCCAAA GCTGCGAGCA AGCCCCAGCA GGAGGAGGCG GAGGTGGAGG GAGTGACTCA 
GAAGCCGAAT CTTCCCAGTC CAGCTTAGAT CTAAGGAGAG AAGGGTCACT TTCTCCAGTG 
AACTCACAAA AAATCACCTT GCTGCTGCAG TCCCCAGCGG TCAAGTTCAT CACCAACCCC 
GAGTTCTTCA CTGTGCTACA TGCCAATTAT AGTGCCTACC GAGTCTTCAC CAGTAGCACC 
TGCTTAAAGC ACATGATTCT GAAAGTCCGA CGGGATGCTC GCAATTTTGA ACGCTACCAG 
CACAACCGGG ACTTGGTGAA TTTCATCAAC ATGTTCGCAG ACACTCGGCT GGAACTGCCC 
CGGGGCTGGG AGATCAAAAC GGACCAGCAG GGAAAGTCTT TTTTCGTGGA CCACAACAGT 
CGAGCTACCA CTTTCATTGA CCCCCGAATC CCTCTTCAGA ACGGTCGTCT TCCCAATCAT 
CTAACTCACC GACAGCACCT CCAGAGGCTC CGAAGTTACA GCGCTGGAGA GGCCTCAGAA 
GTTTCTAGAA ACAGAGGAGC CTCTTTACTG GCCAGGCCAG GACACAGCTT AGTAGCTGCT 
ATTCGAAGCC AACATCAACA TGAGTCATTG CCACTGGCAT ATAATGACAA GATTGTGGCA 
TTTCTTCGCC AGCCAAACAT TTTTGAAATG CTGCAAGAGC GTCAGCCAAG CTTAGCAAGA 
AACCACACAC TCAGGGAGAA AATCCATTAC ATTCGGACTG AGGGTAATCA CGGGCTTGAG 
AAGTTGTCCT GTGATGCGGA TCTGGTCATT TTGCTGAGTC TCTTTGAAGA AGAGATTATG 
TCCTACGTCC CCCTGCAGGC TGCCTTCCAC CCTGGGTATA GCTTCTCTCC CCGATGTTCA 
CCCTGTTCTT CACCTCAGAA CTCCCCAGGT TTACAGAGAG CCAGTGCAAG AGCCCCTTCC 
CCCTACCGAA GAGACTTTGA GGCCAAGCTC CGCAATTTCT ACAGAAAACT GGAAGCCAAA 
GGATTTGGTC AGGGTCCGGG GAAAATTAAG CTCATTATTC GCCGGGATCA TTTGTTGGAG 
GGAACCTTCA ATCAGGTGAT GGCCTATTCG CGGAAAGAGC TCCAGCGAAA CAAGCTCTAC 
GTCACCTTTG TTGGAGAGGA GGGCCTGGAC TACAGTGGCC CCTCGCGGGA GTTCTTCTTC 
CTTCTGTCTC AGGAGCTCTT CAACCCTTAC TATGGACTCT TTGAGTACTC GGCAAATGAT 
ACTTACACGG TGCAGATCAG CCCCATGTCC GCATTTGTAG AAAACCATCT TGAGTGGTTC 
AGGTTTAGCG GTCGCATCCT GGGTCTGGCT CTGATCCATC AGTACCTTCT TGACGCTTTC 
TTCACGAGGC CCTTCTACAA GGCACTCCTG AGACTGCCCT GTGATTTGAG TGACCTGGAA 
TATTTGGATG AGGAATTCCA CCAGAGTTTG CAGTGGATGA AGGACAACAA CATCACAGAC 
ATCTTAGACC TCACTTTCAC TGTTAATGAA GAGGTTTTTG GACAGGTCAC GGAAAGGGAG 
TTGAAGTCTG GAGGAGCCAA CACACAGGTG ACGGAGAAAA ACAAGAAGGA GTACATCGAG 
CGCATGGTGA AGTGGCGGGT GGAGCGCGGC GTGGTACAGC AGACCGAGGC GCTGGTGCGC 
GGCTTCTACG AGGTTGTAGA CTCGAGGCTG GTGTCCGTGT TTGATGCCAG GGAGCTGGAG 
CTGGTGATAG CTGGCACCGC GGAAATCGAC CTAAATGACT GGCGGAATAA CACTGAGTAC 
CGGGGAGGTT ACCACGATGG GCATCTTGTG ATCCGCTGGT TCTGGGCTGC GGTGGAGCGC 
TTCAATAATG AGCAGAGGCT GAGATTACTG CAGTTTGTCA CGGGAACATC CAGCGTGCCC 
TACGAAGGCT TCGCAGCCCT CCGTGGGAGC AATGGGCTTC GGCGCTTCTG CATAGAGAAA 
TGGGGGAAAA TTACTTCTCT CCCCAGGGCA CACACATGCT TCAACCGACT GGATCTTCCA 
CCGTATCCCT CGTACTCCAT GTTGTATGAA AAGCTGTTAA CAGCAGTAGA GGAAACCAGC 
ACCTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867376.1
CDS	529..5346
Translation	MPYPKRPTVKDPGSVVVQGVSKQVARSAFLISRLTPAVAITNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017011887.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011887.1

ORF Insert Sequence:

ATGCCCTACC CGAAAAGGCC AACCGTTAAA GACCCCGGCA GTGTTGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC ATCAGCAGAC TCACTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCCGCCA TGGCGTCTCC TTCTAGAAAC 
TCCCAGAGCC GACGCCGGTG CAAGGAGCCG CTCCGATACA GCTACAACCC CGACCAGTTC 
CACAACATGG ACCTCAGGGG CGGCCCCCAC GATGGCGTCA CCATTCCCCG CTCCACCAGC 
GACACTGACC TGGTCACCTC GGACAGCCGC TCCACGCTCA TGGTCAGCAG CTCCTACTAT 
TCCATCGGGC ACTCTCAGGA CCTGGTCATC CACTGGGACA TAAAGGAGGA AGTGGACGCT 
GGGGACTGGA TTGGCATGTA CCTCATTGAT GAGGTCTTGT CCGAAAACTT TCTGGACTAT 
AAAAACCGTG GAGTCAATGG TTCTCATCGG GGCCAGATCA TCTGGAAGAT CGATGCCAGC 
TCGTACTTTG TGGAACCTGA AACTAAGATC TGCTTCAAAT ACTACCATGG AGTGAGTGGG 
GCCCTGCGAG CAACCACCCC CAGTGTCACG GTCAAAAACT CGGCAGCTCC TATTTTTAAA 
AGCATTGGTG CTGATGAGAC CGTCCAAGGA CAAGGAAGTC GGAGGCTGAT CAGCTTCTCT 
CTCTCAGATT TCCAAGCCAT GGGGTTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAT 
CTGAAGATTT CCATTCAGCC TGGGAAACAC AGCATCTTCC CCGCCCTCCC TCACCATGGA 
CAGGAGAGGA GATCCAAGAT CATAGGCAAC ACCGTGAACC CCATCTGGCA GGCCGAGCAA 
TTCAGTTTTG TGTCCTTGCC CACTGACGTG CTGGAAATTG AGGTGAAGGA CAAGTTTGCC 
AAGAGCCGCC CCATCATCAA GCGCTTCTTG GGAAAGCTGT CGATGCCCGT TCAAAGACTC 
CTGGAGAGAC ACGCCATAGG GGATAGGGTG GTCAGCTACA CACTTGGCCG CAGGCTTCCA 
ACAGATCATG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCCTC CATCCACCCA 
GCAGATGATG AGGAGATTTC CCTGAGTACC GAGCCTGAGT CAGCCCAAAT TCAGGACAGC 
CCCATGAACA ACCTGATGGA AAGCGGCAGT GGGGAACCTC GGTCTGAGGC ACCAGAGTCC 
TCTGAGAGCT GGAAGCCAGA GCAGCTGGGT GAGGGCAGTG TCCCCGATGG TCCAGGGAAC 
CAAAGCATAG AGCTTTCCAG ACCAGCTGAG GAAGCAGCAG TCATCACGGA GGCAGGAGAC 
CAGGGCATGG TCTCTGTGGG ACCTGAAGGG GCTGGGGAGC TCCTGGCCCA GGTGCAAAAG 
GACATCCAGC CTGCCCCCAG TGCAGAAGAG CTGGCCGAGC AGCTGGACCT GGGTGAGGAG 
GCATCAGCAC TGCTGCTGGA AGACGGTGAA GCCCCAGCCA GCACCAAGGA GGAGCCCTTG 
GAGGAGGAAG CAACGACCCA GAGCCGGGCT GGAAGGGAAG AAGAGGAGAA GGAGCAGGAG 
GAGGAGGGAG ATGTGTCTAC CCTGGAGCAG GGAGAGGGCA GGCTGCAGCT GCGGGCCTCG 
GTGAAGAGAA AAAGCAGGCC CTGCTCCTTG CCTGTGTCCG AGCTGGAGAC GGTGATCGCG 
TCAGCCTGCG GGGACCCCGA GACCCCGCGG ACACACTACA TCCGCATCCA CACCCTGCTG 
CACAGCATGC CCTCCGCCCA GGGCGGCAGC GCGGCAGAGG AGGAGGACGG CGCGGAGGAG 
GAGTCCACCC TCAAGGACTC CTCGGAGAAG GATGGGCTCA GCGAGGTGGA CACGGTGGCC 
GCTGACCCGT CTGCCCTGGA AGAGGACAGA GAAGAGCCCG AGGGGGCTAC TCCAGGCACG 
GCGCACCCTG GCCACTCCGG GGGCCACTTC CCCAGCCTGG CCAATGGCGC GGCCCAGGAT 
GGCGACACGC ACCCCAGCAC CGGGAGCGAG AGCGACTCCA GCCCCAGGCA AGGCGGGGAC 
CACAGTTGCG AGGGCTGTGA CGCGTCCTGC TGCAGCCCCT CGTGCTACAG CTCCTCGTGC 
TACAGCACGT CCTGCTACAG CAGCTCGTGC TACAGCGCCT CGTGCTACAG CCCCTCCTGC 
TACAACGGCA ACAGGTTCGC CAGCCACACG CGCTTCTCCT CCGTGGACAG CGCCAAGATC 
TCCGAGAGCA CGGTCTTCTC CTCGCAAGAC GACGAGGAGG AGGAGAACAG CGCGTTCGAG 
TCGGTACCCG ACTCCATGCA GAGCCCTGAG CTGGACCCGG AGTCCACGAA CGGCGCTGGG 
CCGTGGCAAG ACGAGCTGGC CGCCCCTAGC GGGCACGTGG AAAGAAGCCC GGAAGGTCTG 
GAATCCCCCG TGGCAGGTCC AAGCAATCGG AGAGAAGACT GGGAAGCTCG AATTGACAGC 
CACGGGCGGG TCTTTTATGT GGACCACGTG AACCGCACAA CCACCTGGCA GCGTCCGACG 
GCAGCAGCCA CCCCGGATGG CATGCGGAGA TCGGGGTCCA TCCAGCAGAT GGAGCAACTC 
AACAGGCGGT ATCAAAACAT TCAGCGAACC ATTGCAACAG AGAGGTCCGA AGAAGATTCT 
GGCAGCCAAA GCTGCGAGCA AGCCCCAGCA GGAGGAGGCG GAGGTGGAGG GAGTGACTCA 
GAAGCCGAAT CTTCCCAGTC CAGCTTAGAT CTAAGGAGAG AAGGGTCACT TTCTCCAGTG 
AACTCACAAA AAATCACCTT GCTGCTGCAG TCCCCAGCGG TCAAGTTCAT CACCAACCCC 
GAGTTCTTCA CTGTGCTACA TGCCAATTAT AGTGCCTACC GAGTCTTCAC CAGTAGCACC 
TGCTTAAAGC ACATGATTCT GAAAGTCCGA CGGGATGCTC GCAATTTTGA ACGCTACCAG 
CACAACCGGG ACTTGGTGAA TTTCATCAAC ATGTTCGCAG ACACTCGGCT GGAACTGCCC 
CGGGGCTGGG AGATCAAAAC GGACCAGCAG GGAAAGTCTT TTTTCGTGGA CCACAACAGT 
CGAGCTACCA CTTTCATTGA CCCCCGAATC CCTCTTCAGA ACGGTCGTCT TCCCAATCAT 
CTAACTCACC GACAGCACCT CCAGAGGCTC CGAAGTTACA GCGCTGGAGA GGCCTCAGAA 
GTTTCTAGAA ACAGAGGAGC CTCTTTACTG GCCAGGCCAG GACACAGCTT AGTAGCTGCT 
ATTCGAAGCC AACATCAACA TGAGTCATTG CCACTGGCAT ATAATGACAA GATTGTGGCA 
TTTCTTCGCC AGCCAAACAT TTTTGAAATG CTGCAAGAGC GTCAGCCAAG CTTAGCAAGA 
AACCACACAC TCAGGGAGAA AATCCATTAC ATTCGGACTG AGGGTAATCA CGGGCTTGAG 
AAGTTGTCCT GTGATGCGGA TCTGGTCATT TTGCTGAGTC TCTTTGAAGA AGAGATTATG 
TCCTACGTCC CCCTGCAGGC TGCCTTCCAC CCTGGGTATA GCTTCTCTCC CCGATGTTCA 
CCCTGTTCTT CACCTCAGAA CTCCCCAGGT TTACAGAGAG CCAGTGCAAG AGCCCCTTCC 
CCCTACCGAA GAGACTTTGA GGCCAAGCTC CGCAATTTCT ACAGAAAACT GGAAGCCAAA 
GGATTTGGTC AGGGTCCGGG GAAAATTAAG CTCATTATTC GCCGGGATCA TTTGTTGGAG 
GGAACCTTCA ATCAGGTGAT GGCCTATTCG CGGAAAGAGC TCCAGCGAAA CAAGCTCTAC 
GTCACCTTTG TTGGAGAGGA GGGCCTGGAC TACAGTGGCC CCTCGCGGGA GTTCTTCTTC 
CTTCTGTCTC AGGAGCTCTT CAACCCTTAC TATGGACTCT TTGAGTACTC GGCAAATGAT 
ACTTACACGG TGCAGATCAG CCCCATGTCC GCATTTGTAG AAAACCATCT TGAGTGGTTC 
AGGTTTAGCG GTCGCATCCT GGGTCTGGCT CTGATCCATC AGTACCTTCT TGACGCTTTC 
TTCACGAGGC CCTTCTACAA GGCACTCCTG AGACTGCCCT GTGATTTGAG TGACCTGGAA 
TATTTGGATG AGGAATTCCA CCAGAGTTTG CAGTGGATGA AGGACAACAA CATCACAGAC 
ATCTTAGACC TCACTTTCAC TGTTAATGAA GAGGTTTTTG GACAGGTCAC GGAAAGGGAG 
TTGAAGTCTG GAGGAGCCAA CACACAGGTG ACGGAGAAAA ACAAGAAGGA GTACATCGAG 
CGCATGGTGA AGTGGCGGGT GGAGCGCGGC GTGGTACAGC AGACCGAGGC GCTGGTGCGC 
GGCTTCTACG AGGTTGTAGA CTCGAGGCTG GTGTCCGTGT TTGATGCCAG GGAGCTGGAG 
CTGGTGATAG CTGGCACCGC GGAAATCGAC CTAAATGACT GGCGGAATAA CACTGAGTAC 
CGGGGAGGTT ACCACGATGG GCATCTTGTG ATCCGCTGGT TCTGGGCTGC GGTGGAGCGC 
TTCAATAATG AGCAGAGGCT GAGATTACTG CAGTTTGTCA CGGGAACATC CAGCGTGCCC 
TACGAAGGCT TCGCAGCCCT CCGTGGGAGC AATGGGCTTC GGCGCTTCTG CATAGAGAAA 
TGGGGGAAAA TTACTTCTCT CCCCAGGGCA CACACATGCT TCAACCGACT GGATCTTCCA 
CCGTATCCCT CGTACTCCAT GTTGTATGAA AAGCTGTTAA CAGCAGTAGA GGAAACCAGC 
ACCTTTGGAC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017011888.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867377.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu97117
Clone ID Related Accession (Same CDS sequence)	XM_017011888.1
Accession Version	XM_017011888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTACC CGAAAAGGCC AACCGTTAAA GACCCCGGCA GTGTTGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC ATCAGCAGAC TCACTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCCGCCA TGGCGTCTCC TTCTAGAAAC 
TCCCAGAGCC GACGCCGGTG CAAGGAGCCG CTCCGATACA GCTACAACCC CGACCAGTTC 
CACAACATGG ACCTCAGGGG CGGCCCCCAC GATGGCGTCA CCATTCCCCG CTCCACCAGC 
GACACTGACC TGGTCACCTC GGACAGCCGC TCCACGCTCA TGGTCAGCAG CTCCTACTAT 
TCCATCGGGC ACTCTCAGGA CCTGGTCATC CACTGGGACA TAAAGGAGGA AGTGGACGCT 
GGGGACTGGA TTGGCATGTA CCTCATTGAT GAGGTCTTGT CCGAAAACTT TCTGGACTAT 
AAAAACCGTG GAGTCAATGG TTCTCATCGG GGCCAGATCA TCTGGAAGAT CGATGCCAGC 
TCGTACTTTG TGGAACCTGA AACTAAGATC TGCTTCAAAT ACTACCATGG AGTGAGTGGG 
GCCCTGCGAG CAACCACCCC CAGTGTCACG GTCAAAAACT CGGCAGCTCC TATTTTTAAA 
AGCATTGGTG CTGATGAGAC CGTCCAAGGA CAAGGAAGTC GGAGGCTGAT CAGCTTCTCT 
CTCTCAGATT TCCAAGCCAT GGGGTTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAT 
CTGAAGATTT CCATTCAGCC TGGGAAACAC AGCATCTTCC CCGCCCTCCC TCACCATGGA 
CAGGAGAGGA GATCCAAGAT CATAGGCAAC ACCGTGAACC CCATCTGGCA GGCCGAGCAA 
TTCAGTTTTG TGTCCTTGCC CACTGACGTG CTGGAAATTG AGGTGAAGGA CAAGTTTGCC 
AAGAGCCGCC CCATCATCAA GCGCTTCTTG GGAAAGCTGT CGATGCCCGT TCAAAGACTC 
CTGGAGAGAC ACGCCATAGG GGATAGGGTG GTCAGCTACA CACTTGGCCG CAGGCTTCCA 
ACAGATCATG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCCTC CATCCACCCA 
GATGATGAGG AGATTTCCCT GAGTACCGAG CCTGAGTCAG CCCAAATTCA GGACAGCCCC 
ATGAACAACC TGATGGAAAG CGGCAGTGGG GAACCTCGGT CTGAGGCACC AGAGTCCTCT 
GAGAGCTGGA AGCCAGAGCA GCTGGGTGAG GGCAGTGTCC CCGATGGTCC AGGGAACCAA 
AGCATAGAGC TTTCCAGACC AGCTGAGGAA GCAGCAGTCA TCACGGAGGC AGGAGACCAG 
GGCATGGTCT CTGTGGGACC TGAAGGGGCT GGGGAGCTCC TGGCCCAGGT GCAAAAGGAC 
ATCCAGCCTG CCCCCAGTGC AGAAGAGCTG GCCGAGCAGC TGGACCTGGG TGAGGAGGCA 
TCAGCACTGC TGCTGGAAGA CGGTGAAGCC CCAGCCAGCA CCAAGGAGGA GCCCTTGGAG 
GAGGAAGCAA CGACCCAGAG CCGGGCTGGA AGGGAAGAAG AGGAGAAGGA GCAGGAGGAG 
GAGGGAGATG TGTCTACCCT GGAGCAGGGA GAGGGCAGGC TGCAGCTGCG GGCCTCGGTG 
AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT GTGTCCGAGC TGGAGACGGT GATCGCGTCA 
GCCTGCGGGG ACCCCGAGAC CCCGCGGACA CACTACATCC GCATCCACAC CCTGCTGCAC 
AGCATGCCCT CCGCCCAGGG CGGCAGCGCG GCAGAGGAGG AGGACGGCGC GGAGGAGGAG 
TCCACCCTCA AGGACTCCTC GGAGAAGGAT GGGCTCAGCG AGGTGGACAC GGTGGCCGCT 
GACCCGTCTG CCCTGGAAGA GGACAGAGAA GAGCCCGAGG GGGCTACTCC AGGCACGGCG 
CACCCTGGCC ACTCCGGGGG CCACTTCCCC AGCCTGGCCA ATGGCGCGGC CCAGGATGGC 
GACACGCACC CCAGCACCGG GAGCGAGAGC GACTCCAGCC CCAGGCAAGG CGGGGACCAC 
AGTTGCGAGG GCTGTGACGC GTCCTGCTGC AGCCCCTCGT GCTACAGCTC CTCGTGCTAC 
AGCACGTCCT GCTACAGCAG CTCGTGCTAC AGCGCCTCGT GCTACAGCCC CTCCTGCTAC 
AACGGCAACA GGTTCGCCAG CCACACGCGC TTCTCCTCCG TGGACAGCGC CAAGATCTCC 
GAGAGCACGG TCTTCTCCTC GCAAGACGAC GAGGAGGAGG AGAACAGCGC GTTCGAGTCG 
GTACCCGACT CCATGCAGAG CCCTGAGCTG GACCCGGAGT CCACGAACGG CGCTGGGCCG 
TGGCAAGACG AGCTGGCCGC CCCTAGCGGG CACGTGGAAA GAAGCCCGGA AGGTCTGGAA 
TCCCCCGTGG CAGGTCCAAG CAATCGGAGA GAAGACTGGG AAGCTCGAAT TGACAGCCAC 
GGGCGGGTCT TTTATGTGGA CCACGTGAAC CGCACAACCA CCTGGCAGCG TCCGACGGCA 
GCAGCCACCC CGGATGGCAT GCGGAGATCG GGGTCCATCC AGCAGATGGA GCAACTCAAC 
AGGCGGTATC AAAACATTCA GCGAACCATT GCAACAGAGA GGTCCGAAGA AGATTCTGGC 
AGCCAAAGCT GCGAGCAAGC CCCAGCAGGA GGAGGCGGAG GTGGAGGGAG TGACTCAGAA 
GCCGAATCTT CCCAGTCCAG CTTAGATCTA AGGAGAGAAG GGTCACTTTC TCCAGTGAAC 
TCACAAAAAA TCACCTTGCT GCTGCAGTCC CCAGCGGTCA AGTTCATCAC CAACCCCGAG 
TTCTTCACTG TGCTACATGC CAATTATAGT GCCTACCGAG TCTTCACCAG TAGCACCTGC 
TTAAAGCACA TGATTCTGAA AGTCCGACGG GATGCTCGCA ATTTTGAACG CTACCAGCAC 
AACCGGGACT TGGTGAATTT CATCAACATG TTCGCAGACA CTCGGCTGGA ACTGCCCCGG 
GGCTGGGAGA TCAAAACGGA CCAGCAGGGA AAGTCTTTTT TCGTGGACCA CAACAGTCGA 
GCTACCACTT TCATTGACCC CCGAATCCCT CTTCAGAACG GTCGTCTTCC CAATCATCTA 
ACTCACCGAC AGCACCTCCA GAGGCTCCGA AGTTACAGCG CTGGAGAGGC CTCAGAAGTT 
TCTAGAAACA GAGGAGCCTC TTTACTGGCC AGGCCAGGAC ACAGCTTAGT AGCTGCTATT 
CGAAGCCAAC ATCAACATGA GTCATTGCCA CTGGCATATA ATGACAAGAT TGTGGCATTT 
CTTCGCCAGC CAAACATTTT TGAAATGCTG CAAGAGCGTC AGCCAAGCTT AGCAAGAAAC 
CACACACTCA GGGAGAAAAT CCATTACATT CGGACTGAGG GTAATCACGG GCTTGAGAAG 
TTGTCCTGTG ATGCGGATCT GGTCATTTTG CTGAGTCTCT TTGAAGAAGA GATTATGTCC 
TACGTCCCCC TGCAGGCTGC CTTCCACCCT GGGTATAGCT TCTCTCCCCG ATGTTCACCC 
TGTTCTTCAC CTCAGAACTC CCCAGGTTTA CAGAGAGCCA GTGCAAGAGC CCCTTCCCCC 
TACCGAAGAG ACTTTGAGGC CAAGCTCCGC AATTTCTACA GAAAACTGGA AGCCAAAGGA 
TTTGGTCAGG GTCCGGGGAA AATTAAGCTC ATTATTCGCC GGGATCATTT GTTGGAGGGA 
ACCTTCAATC AGGTGATGGC CTATTCGCGG AAAGAGCTCC AGCGAAACAA GCTCTACGTC 
ACCTTTGTTG GAGAGGAGGG CCTGGACTAC AGTGGCCCCT CGCGGGAGTT CTTCTTCCTT 
CTGTCTCAGG AGCTCTTCAA CCCTTACTAT GGACTCTTTG AGTACTCGGC AAATGATACT 
TACACGGTGC AGATCAGCCC CATGTCCGCA TTTGTAGAAA ACCATCTTGA GTGGTTCAGG 
TTTAGCGGTC GCATCCTGGG TCTGGCTCTG ATCCATCAGT ACCTTCTTGA CGCTTTCTTC 
ACGAGGCCCT TCTACAAGGC ACTCCTGAGA CTGCCCTGTG ATTTGAGTGA CCTGGAATAT 
TTGGATGAGG AATTCCACCA GAGTTTGCAG TGGATGAAGG ACAACAACAT CACAGACATC 
TTAGACCTCA CTTTCACTGT TAATGAAGAG GTTTTTGGAC AGGTCACGGA AAGGGAGTTG 
AAGTCTGGAG GAGCCAACAC ACAGGTGACG GAGAAAAACA AGAAGGAGTA CATCGAGCGC 
ATGGTGAAGT GGCGGGTGGA GCGCGGCGTG GTACAGCAGA CCGAGGCGCT GGTGCGCGGC 
TTCTACGAGG TTGTAGACTC GAGGCTGGTG TCCGTGTTTG ATGCCAGGGA GCTGGAGCTG 
GTGATAGCTG GCACCGCGGA AATCGACCTA AATGACTGGC GGAATAACAC TGAGTACCGG 
GGAGGTTACC ACGATGGGCA TCTTGTGATC CGCTGGTTCT GGGCTGCGGT GGAGCGCTTC 
AATAATGAGC AGAGGCTGAG ATTACTGCAG TTTGTCACGG GAACATCCAG CGTGCCCTAC 
GAAGGCTTCG CAGCCCTCCG TGGGAGCAAT GGGCTTCGGC GCTTCTGCAT AGAGAAATGG 
GGGAAAATTA CTTCTCTCCC CAGGGCACAC ACATGCTTCA ACCGACTGGA TCTTCCACCG 
TATCCCTCGT ACTCCATGTT GTATGAAAAG CTGTTAACAG CAGTAGAGGA AACCAGCACC 
TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867377.1
CDS	529..5343
Translation	MPYPKRPTVKDPGSVVVQGVSKQVARSAFLISRLTPAVAITNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_017011888.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011888.1

ORF Insert Sequence:

ATGCCCTACC CGAAAAGGCC AACCGTTAAA GACCCCGGCA GTGTTGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC ATCAGCAGAC TCACTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCCGCCA TGGCGTCTCC TTCTAGAAAC 
TCCCAGAGCC GACGCCGGTG CAAGGAGCCG CTCCGATACA GCTACAACCC CGACCAGTTC 
CACAACATGG ACCTCAGGGG CGGCCCCCAC GATGGCGTCA CCATTCCCCG CTCCACCAGC 
GACACTGACC TGGTCACCTC GGACAGCCGC TCCACGCTCA TGGTCAGCAG CTCCTACTAT 
TCCATCGGGC ACTCTCAGGA CCTGGTCATC CACTGGGACA TAAAGGAGGA AGTGGACGCT 
GGGGACTGGA TTGGCATGTA CCTCATTGAT GAGGTCTTGT CCGAAAACTT TCTGGACTAT 
AAAAACCGTG GAGTCAATGG TTCTCATCGG GGCCAGATCA TCTGGAAGAT CGATGCCAGC 
TCGTACTTTG TGGAACCTGA AACTAAGATC TGCTTCAAAT ACTACCATGG AGTGAGTGGG 
GCCCTGCGAG CAACCACCCC CAGTGTCACG GTCAAAAACT CGGCAGCTCC TATTTTTAAA 
AGCATTGGTG CTGATGAGAC CGTCCAAGGA CAAGGAAGTC GGAGGCTGAT CAGCTTCTCT 
CTCTCAGATT TCCAAGCCAT GGGGTTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAT 
CTGAAGATTT CCATTCAGCC TGGGAAACAC AGCATCTTCC CCGCCCTCCC TCACCATGGA 
CAGGAGAGGA GATCCAAGAT CATAGGCAAC ACCGTGAACC CCATCTGGCA GGCCGAGCAA 
TTCAGTTTTG TGTCCTTGCC CACTGACGTG CTGGAAATTG AGGTGAAGGA CAAGTTTGCC 
AAGAGCCGCC CCATCATCAA GCGCTTCTTG GGAAAGCTGT CGATGCCCGT TCAAAGACTC 
CTGGAGAGAC ACGCCATAGG GGATAGGGTG GTCAGCTACA CACTTGGCCG CAGGCTTCCA 
ACAGATCATG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCCTC CATCCACCCA 
GATGATGAGG AGATTTCCCT GAGTACCGAG CCTGAGTCAG CCCAAATTCA GGACAGCCCC 
ATGAACAACC TGATGGAAAG CGGCAGTGGG GAACCTCGGT CTGAGGCACC AGAGTCCTCT 
GAGAGCTGGA AGCCAGAGCA GCTGGGTGAG GGCAGTGTCC CCGATGGTCC AGGGAACCAA 
AGCATAGAGC TTTCCAGACC AGCTGAGGAA GCAGCAGTCA TCACGGAGGC AGGAGACCAG 
GGCATGGTCT CTGTGGGACC TGAAGGGGCT GGGGAGCTCC TGGCCCAGGT GCAAAAGGAC 
ATCCAGCCTG CCCCCAGTGC AGAAGAGCTG GCCGAGCAGC TGGACCTGGG TGAGGAGGCA 
TCAGCACTGC TGCTGGAAGA CGGTGAAGCC CCAGCCAGCA CCAAGGAGGA GCCCTTGGAG 
GAGGAAGCAA CGACCCAGAG CCGGGCTGGA AGGGAAGAAG AGGAGAAGGA GCAGGAGGAG 
GAGGGAGATG TGTCTACCCT GGAGCAGGGA GAGGGCAGGC TGCAGCTGCG GGCCTCGGTG 
AAGAGAAAAA GCAGGCCCTG CTCCTTGCCT GTGTCCGAGC TGGAGACGGT GATCGCGTCA 
GCCTGCGGGG ACCCCGAGAC CCCGCGGACA CACTACATCC GCATCCACAC CCTGCTGCAC 
AGCATGCCCT CCGCCCAGGG CGGCAGCGCG GCAGAGGAGG AGGACGGCGC GGAGGAGGAG 
TCCACCCTCA AGGACTCCTC GGAGAAGGAT GGGCTCAGCG AGGTGGACAC GGTGGCCGCT 
GACCCGTCTG CCCTGGAAGA GGACAGAGAA GAGCCCGAGG GGGCTACTCC AGGCACGGCG 
CACCCTGGCC ACTCCGGGGG CCACTTCCCC AGCCTGGCCA ATGGCGCGGC CCAGGATGGC 
GACACGCACC CCAGCACCGG GAGCGAGAGC GACTCCAGCC CCAGGCAAGG CGGGGACCAC 
AGTTGCGAGG GCTGTGACGC GTCCTGCTGC AGCCCCTCGT GCTACAGCTC CTCGTGCTAC 
AGCACGTCCT GCTACAGCAG CTCGTGCTAC AGCGCCTCGT GCTACAGCCC CTCCTGCTAC 
AACGGCAACA GGTTCGCCAG CCACACGCGC TTCTCCTCCG TGGACAGCGC CAAGATCTCC 
GAGAGCACGG TCTTCTCCTC GCAAGACGAC GAGGAGGAGG AGAACAGCGC GTTCGAGTCG 
GTACCCGACT CCATGCAGAG CCCTGAGCTG GACCCGGAGT CCACGAACGG CGCTGGGCCG 
TGGCAAGACG AGCTGGCCGC CCCTAGCGGG CACGTGGAAA GAAGCCCGGA AGGTCTGGAA 
TCCCCCGTGG CAGGTCCAAG CAATCGGAGA GAAGACTGGG AAGCTCGAAT TGACAGCCAC 
GGGCGGGTCT TTTATGTGGA CCACGTGAAC CGCACAACCA CCTGGCAGCG TCCGACGGCA 
GCAGCCACCC CGGATGGCAT GCGGAGATCG GGGTCCATCC AGCAGATGGA GCAACTCAAC 
AGGCGGTATC AAAACATTCA GCGAACCATT GCAACAGAGA GGTCCGAAGA AGATTCTGGC 
AGCCAAAGCT GCGAGCAAGC CCCAGCAGGA GGAGGCGGAG GTGGAGGGAG TGACTCAGAA 
GCCGAATCTT CCCAGTCCAG CTTAGATCTA AGGAGAGAAG GGTCACTTTC TCCAGTGAAC 
TCACAAAAAA TCACCTTGCT GCTGCAGTCC CCAGCGGTCA AGTTCATCAC CAACCCCGAG 
TTCTTCACTG TGCTACATGC CAATTATAGT GCCTACCGAG TCTTCACCAG TAGCACCTGC 
TTAAAGCACA TGATTCTGAA AGTCCGACGG GATGCTCGCA ATTTTGAACG CTACCAGCAC 
AACCGGGACT TGGTGAATTT CATCAACATG TTCGCAGACA CTCGGCTGGA ACTGCCCCGG 
GGCTGGGAGA TCAAAACGGA CCAGCAGGGA AAGTCTTTTT TCGTGGACCA CAACAGTCGA 
GCTACCACTT TCATTGACCC CCGAATCCCT CTTCAGAACG GTCGTCTTCC CAATCATCTA 
ACTCACCGAC AGCACCTCCA GAGGCTCCGA AGTTACAGCG CTGGAGAGGC CTCAGAAGTT 
TCTAGAAACA GAGGAGCCTC TTTACTGGCC AGGCCAGGAC ACAGCTTAGT AGCTGCTATT 
CGAAGCCAAC ATCAACATGA GTCATTGCCA CTGGCATATA ATGACAAGAT TGTGGCATTT 
CTTCGCCAGC CAAACATTTT TGAAATGCTG CAAGAGCGTC AGCCAAGCTT AGCAAGAAAC 
CACACACTCA GGGAGAAAAT CCATTACATT CGGACTGAGG GTAATCACGG GCTTGAGAAG 
TTGTCCTGTG ATGCGGATCT GGTCATTTTG CTGAGTCTCT TTGAAGAAGA GATTATGTCC 
TACGTCCCCC TGCAGGCTGC CTTCCACCCT GGGTATAGCT TCTCTCCCCG ATGTTCACCC 
TGTTCTTCAC CTCAGAACTC CCCAGGTTTA CAGAGAGCCA GTGCAAGAGC CCCTTCCCCC 
TACCGAAGAG ACTTTGAGGC CAAGCTCCGC AATTTCTACA GAAAACTGGA AGCCAAAGGA 
TTTGGTCAGG GTCCGGGGAA AATTAAGCTC ATTATTCGCC GGGATCATTT GTTGGAGGGA 
ACCTTCAATC AGGTGATGGC CTATTCGCGG AAAGAGCTCC AGCGAAACAA GCTCTACGTC 
ACCTTTGTTG GAGAGGAGGG CCTGGACTAC AGTGGCCCCT CGCGGGAGTT CTTCTTCCTT 
CTGTCTCAGG AGCTCTTCAA CCCTTACTAT GGACTCTTTG AGTACTCGGC AAATGATACT 
TACACGGTGC AGATCAGCCC CATGTCCGCA TTTGTAGAAA ACCATCTTGA GTGGTTCAGG 
TTTAGCGGTC GCATCCTGGG TCTGGCTCTG ATCCATCAGT ACCTTCTTGA CGCTTTCTTC 
ACGAGGCCCT TCTACAAGGC ACTCCTGAGA CTGCCCTGTG ATTTGAGTGA CCTGGAATAT 
TTGGATGAGG AATTCCACCA GAGTTTGCAG TGGATGAAGG ACAACAACAT CACAGACATC 
TTAGACCTCA CTTTCACTGT TAATGAAGAG GTTTTTGGAC AGGTCACGGA AAGGGAGTTG 
AAGTCTGGAG GAGCCAACAC ACAGGTGACG GAGAAAAACA AGAAGGAGTA CATCGAGCGC 
ATGGTGAAGT GGCGGGTGGA GCGCGGCGTG GTACAGCAGA CCGAGGCGCT GGTGCGCGGC 
TTCTACGAGG TTGTAGACTC GAGGCTGGTG TCCGTGTTTG ATGCCAGGGA GCTGGAGCTG 
GTGATAGCTG GCACCGCGGA AATCGACCTA AATGACTGGC GGAATAACAC TGAGTACCGG 
GGAGGTTACC ACGATGGGCA TCTTGTGATC CGCTGGTTCT GGGCTGCGGT GGAGCGCTTC 
AATAATGAGC AGAGGCTGAG ATTACTGCAG TTTGTCACGG GAACATCCAG CGTGCCCTAC 
GAAGGCTTCG CAGCCCTCCG TGGGAGCAAT GGGCTTCGGC GCTTCTGCAT AGAGAAATGG 
GGGAAAATTA CTTCTCTCCC CAGGGCACAC ACATGCTTCA ACCGACTGGA TCTTCCACCG 
TATCCCTCGT ACTCCATGTT GTATGAAAAG CTGTTAACAG CAGTAGAGGA AACCAGCACC 
TTTGGACTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_011515225.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513527.2 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X17, mRNA.

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CloneID	OHu97118
Clone ID Related Accession (Same CDS sequence)	XM_011515225.2
Accession Version	XM_011515225.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515225.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGCTA CCACTGCCAG CTACATCTAT AGAACTTTGA AGGAAACAAA TTGTGATGCA 
AAGAGGTTAT CATTGATGGC TGAAACTAAG ATCTGCTTCA AATACTACCA TGGAGTGAGT 
GGGGCCCTGC GAGCAACCAC CCCCAGTGTC ACGGTCAAAA ACTCGGCAGC TCCTATTTTT 
AAAAGCATTG GTGCTGATGA GACCGTCCAA GGACAAGGAA GTCGGAGGCT GATCAGCTTC 
TCTCTCTCAG ATTTCCAAGC CATGGGGTTG AAGAAAGGGA TGTTTTTCAA CCCAGACCCT 
TATCTGAAGA TTTCCATTCA GCCTGGGAAA CACAGCATCT TCCCCGCCCT CCCTCACCAT 
GGACAGGAGA GGAGATCCAA GATCATAGGC AACACCGTGA ACCCCATCTG GCAGGCCGAG 
CAATTCAGTT TTGTGTCCTT GCCCACTGAC GTGCTGGAAA TTGAGGTGAA GGACAAGTTT 
GCCAAGAGCC GCCCCATCAT CAAGCGCTTC TTGGGAAAGC TGTCGATGCC CGTTCAAAGA 
CTCCTGGAGA GACACGCCAT AGGGGATAGG GTGGTCAGCT ACACACTTGG CCGCAGGCTT 
CCAACAGATC ATGTGAGTGG ACAGCTGCAA TTCCGATTTG AGATCACTTC CTCCATCCAC 
CCAGCAGATG ATGAGGAGAT TTCCCTGAGT ACCGAGCCTG AGTCAGCCCA AATTCAGGAC 
AGCCCCATGA ACAACCTGAT GGAAAGCGGC AGTGGGGAAC CTCGGTCTGA GGCACCAGAG 
TCCTCTGAGA GCTGGAAGCC AGAGCAGCTG GGTGAGGGCA GTGTCCCCGA TGGTCCAGGG 
AACCAAAGCA TAGAGCTTTC CAGACCAGCT GAGGAAGCAG CAGTCATCAC GGAGGCAGGA 
GACCAGGGCA TGGTCTCTGT GGGACCTGAA GGGGCTGGGG AGCTCCTGGC CCAGGTGCAA 
AAGGACATCC AGCCTGCCCC CAGTGCAGAA GAGCTGGCCG AGCAGCTGGA CCTGGGTGAG 
GAGGCATCAG CACTGCTGCT GGAAGACGGT GAAGCCCCAG CCAGCACCAA GGAGGAGCCC 
TTGGAGGAGG AAGCAACGAC CCAGAGCCGG GCTGGAAGGG AAGAAGAGGA GAAGGAGCAG 
GAGGAGGAGG GAGATGTGTC TACCCTGGAG CAGGGAGAGG GCAGGCTGCA GCTGCGGGCC 
TCGGTGAAGA GAAAAAGCAG GCCCTGCTCC TTGCCTGTGT CCGAGCTGGA GACGGTGATC 
GCGTCAGCCT GCGGGGACCC CGAGACCCCG CGGACACACT ACATCCGCAT CCACACCCTG 
CTGCACAGCA TGCCCTCCGC CCAGGGCGGC AGCGCGGCAG AGGAGGAGGA CGGCGCGGAG 
GAGGAGTCCA CCCTCAAGGA CTCCTCGGAG AAGGATGGGC TCAGCGAGGT GGACACGGTG 
GCCGCTGACC CGTCTGCCCT GGAAGAGGAC AGAGAAGAGC CCGAGGGGGC TACTCCAGGC 
ACGGCGCACC CTGGCCACTC CGGGGGCCAC TTCCCCAGCC TGGCCAATGG CGCGGCCCAG 
GATGGCGACA CGCACCCCAG CACCGGGAGC GAGAGCGACT CCAGCCCCAG GCAAGGCGGG 
GACCACAGTT GCGAGGGCTG TGACGCGTCC TGCTGCAGCC CCTCGTGCTA CAGCTCCTCG 
TGCTACAGCA CGTCCTGCTA CAGCAGCTCG TGCTACAGCG CCTCGTGCTA CAGCCCCTCC 
TGCTACAACG GCAACAGGTT CGCCAGCCAC ACGCGCTTCT CCTCCGTGGA CAGCGCCAAG 
ATCTCCGAGA GCACGGTCTT CTCCTCGCAA GACGACGAGG AGGAGGAGAA CAGCGCGTTC 
GAGTCGGTAC CCGACTCCAT GCAGAGCCCT GAGCTGGACC CGGAGTCCAC GAACGGCGCT 
GGGCCGTGGC AAGACGAGCT GGCCGCCCCT AGCGGGCACG TGGAAAGAAG CCCGGAAGGT 
CTGGAATCCC CCGTGGCAGG TCCAAGCAAT CGGAGAGAAG GTGAATGTCC TATACTCCAT 
AATTCCCAGC CAGTAAGCCA GCTTCCTTCC CTGAGGCCTG AACATCATCA CTACCCAACA 
ATCGATGAGC CTCTTCCACC AAACTGGGAA GCTCGAATTG ACAGCCACGG GCGGGTCTTT 
TATGTGGACC ACGTGAACCG CACAACCACC TGGCAGCGTC CGACGGCAGC AGCCACCCCG 
GATGGCATGC GGAGATCGGG GTCCATCCAG CAGATGGAGC AACTCAACAG GCGGTATCAA 
AACATTCAGC GAACCATTGC AACAGAGAGG TCCGAAGAAG ATTCTGGCAG CCAAAGCTGC 
GAGCAAGCCC CAGCAGGAGG AGGCGGAGGT GGAGGGAGTG ACTCAGAAGC CGAATCTTCC 
CAGTCCAGCT TAGATCTAAG GAGAGAAGGG TCACTTTCTC CAGTGAACTC ACAAAAAATC 
ACCTTGCTGC TGCAGTCCCC AGCGGTCAAG TTCATCACCA ACCCCGAGTT CTTCACTGTG 
CTACATGCCA ATTATAGTGC CTACCGAGTC TTCACCAGTA GCACCTGCTT AAAGCACATG 
ATTCTGAAAG TCCGACGGGA TGCTCGCAAT TTTGAACGCT ACCAGCACAA CCGGGACTTG 
GTGAATTTCA TCAACATGTT CGCAGACACT CGGCTGGAAC TGCCCCGGGG CTGGGAGATC 
AAAACGGACC AGCAGGGAAA GTCTTTTTTC GTGGACCACA ACAGTCGAGC TACCACTTTC 
ATTGACCCCC GAATCCCTCT TCAGAACGGT CGTCTTCCCA ATCATCTAAC TCACCGACAG 
CACCTCCAGA GGCTCCGAAG TTACAGCGCT GGAGAGGCCT CAGAAGTTTC TAGAAACAGA 
GGAGCCTCTT TACTGGCCAG GCCAGGACAC AGCTTAGTAG CTGCTATTCG AAGCCAACAT 
CAACATGAGT CATTGCCACT GGCATATAAT GACAAGATTG TGGCATTTCT TCGCCAGCCA 
AACATTTTTG AAATGCTGCA AGAGCGTCAG CCAAGCTTAG CAAGAAACCA CACACTCAGG 
GAGAAAATCC ATTACATTCG GACTGAGGGT AATCACGGGC TTGAGAAGTT GTCCTGTGAT 
GCGGATCTGG TCATTTTGCT GAGTCTCTTT GAAGAAGAGA TTATGTCCTA CGTCCCCCTG 
CAGGCTGCCT TCCACCCTGG GTATAGCTTC TCTCCCCGAT GTTCACCCTG TTCTTCACCT 
CAGAACTCCC CAGGTTTACA GAGAGCCAGT GCAAGAGCCC CTTCCCCCTA CCGAAGAGAC 
TTTGAGGCCA AGCTCCGCAA TTTCTACAGA AAACTGGAAG CCAAAGGATT TGGTCAGGGT 
CCGGGGAAAA TTAAGCTCAT TATTCGCCGG GATCATTTGT TGGAGGGAAC CTTCAATCAG 
GTGATGGCCT ATTCGCGGAA AGAGCTCCAG CGAAACAAGC TCTACGTCAC CTTTGTTGGA 
GAGGAGGGCC TGGACTACAG TGGCCCCTCG CGGGAGTTCT TCTTCCTTCT GTCTCAGGAG 
CTCTTCAACC CTTACTATGG ACTCTTTGAG TACTCGGCAA ATGATACTTA CACGGTGCAG 
ATCAGCCCCA TGTCCGCATT TGTAGAAAAC CATCTTGAGT GGTTCAGGTT TAGCGGTCGC 
ATCCTGGGTC TGGCTCTGAT CCATCAGTAC CTTCTTGACG CTTTCTTCAC GAGGCCCTTC 
TACAAGGCAC TCCTGAGACT GCCCTGTGAT TTGAGTGACC TGGAATATTT GGATGAGGAA 
TTCCACCAGA GTTTGCAGTG GATGAAGGAC AACAACATCA CAGACATCTT AGACCTCACT 
TTCACTGTTA ATGAAGAGGT TTTTGGACAG GTCACGGAAA GGGAGTTGAA GTCTGGAGGA 
GCCAACACAC AGGTGACGGA GAAAAACAAG AAGGAGTACA TCGAGCGCAT GGTGAAGTGG 
CGGGTGGAGC GCGGCGTGGT ACAGCAGACC GAGGCGCTGG TGCGCGGCTT CTACGAGGTT 
GTAGACTCGA GGCTGGTGTC CGTGTTTGAT GCCAGGGAGC TGGAGCTGGT GATAGCTGGC 
ACCGCGGAAA TCGACCTAAA TGACTGGCGG AATAACACTG AGTACCGGGG AGGTTACCAC 
GATGGGCATC TTGTGATCCG CTGGTTCTGG GCTGCGGTGG AGCGCTTCAA TAATGAGCAG 
AGGCTGAGAT TACTGCAGTT TGTCACGGGA ACATCCAGCG TGCCCTACGA AGGCTTCGCA 
GCCCTCCGTG GGAGCAATGG GCTTCGGCGC TTCTGCATAG AGAAATGGGG GAAAATTACT 
TCTCTCCCCA GGGCACACAC ATGCTTCAAC CGACTGGATC TTCCACCGTA TCCCTCGTAC 
TCCATGTTGT ATGAAAAGCT GTTAACAGCA GTAGAGGAAA CCAGCACCTT TGGACTTGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513527.2
CDS	16..4458
Translation	MRATTASYIYRTLKETNCDAKRLSLMAETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPADDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_011515225.2
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515225.2

ORF Insert Sequence:

ATGCGTGCTA CCACTGCCAG CTACATCTAT AGAACTTTGA AGGAAACAAA TTGTGATGCA 
AAGAGGTTAT CATTGATGGC TGAAACTAAG ATCTGCTTCA AATACTACCA TGGAGTGAGT 
GGGGCCCTGC GAGCAACCAC CCCCAGTGTC ACGGTCAAAA ACTCGGCAGC TCCTATTTTT 
AAAAGCATTG GTGCTGATGA GACCGTCCAA GGACAAGGAA GTCGGAGGCT GATCAGCTTC 
TCTCTCTCAG ATTTCCAAGC CATGGGGTTG AAGAAAGGGA TGTTTTTCAA CCCAGACCCT 
TATCTGAAGA TTTCCATTCA GCCTGGGAAA CACAGCATCT TCCCCGCCCT CCCTCACCAT 
GGACAGGAGA GGAGATCCAA GATCATAGGC AACACCGTGA ACCCCATCTG GCAGGCCGAG 
CAATTCAGTT TTGTGTCCTT GCCCACTGAC GTGCTGGAAA TTGAGGTGAA GGACAAGTTT 
GCCAAGAGCC GCCCCATCAT CAAGCGCTTC TTGGGAAAGC TGTCGATGCC CGTTCAAAGA 
CTCCTGGAGA GACACGCCAT AGGGGATAGG GTGGTCAGCT ACACACTTGG CCGCAGGCTT 
CCAACAGATC ATGTGAGTGG ACAGCTGCAA TTCCGATTTG AGATCACTTC CTCCATCCAC 
CCAGCAGATG ATGAGGAGAT TTCCCTGAGT ACCGAGCCTG AGTCAGCCCA AATTCAGGAC 
AGCCCCATGA ACAACCTGAT GGAAAGCGGC AGTGGGGAAC CTCGGTCTGA GGCACCAGAG 
TCCTCTGAGA GCTGGAAGCC AGAGCAGCTG GGTGAGGGCA GTGTCCCCGA TGGTCCAGGG 
AACCAAAGCA TAGAGCTTTC CAGACCAGCT GAGGAAGCAG CAGTCATCAC GGAGGCAGGA 
GACCAGGGCA TGGTCTCTGT GGGACCTGAA GGGGCTGGGG AGCTCCTGGC CCAGGTGCAA 
AAGGACATCC AGCCTGCCCC CAGTGCAGAA GAGCTGGCCG AGCAGCTGGA CCTGGGTGAG 
GAGGCATCAG CACTGCTGCT GGAAGACGGT GAAGCCCCAG CCAGCACCAA GGAGGAGCCC 
TTGGAGGAGG AAGCAACGAC CCAGAGCCGG GCTGGAAGGG AAGAAGAGGA GAAGGAGCAG 
GAGGAGGAGG GAGATGTGTC TACCCTGGAG CAGGGAGAGG GCAGGCTGCA GCTGCGGGCC 
TCGGTGAAGA GAAAAAGCAG GCCCTGCTCC TTGCCTGTGT CCGAGCTGGA GACGGTGATC 
GCGTCAGCCT GCGGGGACCC CGAGACCCCG CGGACACACT ACATCCGCAT CCACACCCTG 
CTGCACAGCA TGCCCTCCGC CCAGGGCGGC AGCGCGGCAG AGGAGGAGGA CGGCGCGGAG 
GAGGAGTCCA CCCTCAAGGA CTCCTCGGAG AAGGATGGGC TCAGCGAGGT GGACACGGTG 
GCCGCTGACC CGTCTGCCCT GGAAGAGGAC AGAGAAGAGC CCGAGGGGGC TACTCCAGGC 
ACGGCGCACC CTGGCCACTC CGGGGGCCAC TTCCCCAGCC TGGCCAATGG CGCGGCCCAG 
GATGGCGACA CGCACCCCAG CACCGGGAGC GAGAGCGACT CCAGCCCCAG GCAAGGCGGG 
GACCACAGTT GCGAGGGCTG TGACGCGTCC TGCTGCAGCC CCTCGTGCTA CAGCTCCTCG 
TGCTACAGCA CGTCCTGCTA CAGCAGCTCG TGCTACAGCG CCTCGTGCTA CAGCCCCTCC 
TGCTACAACG GCAACAGGTT CGCCAGCCAC ACGCGCTTCT CCTCCGTGGA CAGCGCCAAG 
ATCTCCGAGA GCACGGTCTT CTCCTCGCAA GACGACGAGG AGGAGGAGAA CAGCGCGTTC 
GAGTCGGTAC CCGACTCCAT GCAGAGCCCT GAGCTGGACC CGGAGTCCAC GAACGGCGCT 
GGGCCGTGGC AAGACGAGCT GGCCGCCCCT AGCGGGCACG TGGAAAGAAG CCCGGAAGGT 
CTGGAATCCC CCGTGGCAGG TCCAAGCAAT CGGAGAGAAG GTGAATGTCC TATACTCCAT 
AATTCCCAGC CAGTAAGCCA GCTTCCTTCC CTGAGGCCTG AACATCATCA CTACCCAACA 
ATCGATGAGC CTCTTCCACC AAACTGGGAA GCTCGAATTG ACAGCCACGG GCGGGTCTTT 
TATGTGGACC ACGTGAACCG CACAACCACC TGGCAGCGTC CGACGGCAGC AGCCACCCCG 
GATGGCATGC GGAGATCGGG GTCCATCCAG CAGATGGAGC AACTCAACAG GCGGTATCAA 
AACATTCAGC GAACCATTGC AACAGAGAGG TCCGAAGAAG ATTCTGGCAG CCAAAGCTGC 
GAGCAAGCCC CAGCAGGAGG AGGCGGAGGT GGAGGGAGTG ACTCAGAAGC CGAATCTTCC 
CAGTCCAGCT TAGATCTAAG GAGAGAAGGG TCACTTTCTC CAGTGAACTC ACAAAAAATC 
ACCTTGCTGC TGCAGTCCCC AGCGGTCAAG TTCATCACCA ACCCCGAGTT CTTCACTGTG 
CTACATGCCA ATTATAGTGC CTACCGAGTC TTCACCAGTA GCACCTGCTT AAAGCACATG 
ATTCTGAAAG TCCGACGGGA TGCTCGCAAT TTTGAACGCT ACCAGCACAA CCGGGACTTG 
GTGAATTTCA TCAACATGTT CGCAGACACT CGGCTGGAAC TGCCCCGGGG CTGGGAGATC 
AAAACGGACC AGCAGGGAAA GTCTTTTTTC GTGGACCACA ACAGTCGAGC TACCACTTTC 
ATTGACCCCC GAATCCCTCT TCAGAACGGT CGTCTTCCCA ATCATCTAAC TCACCGACAG 
CACCTCCAGA GGCTCCGAAG TTACAGCGCT GGAGAGGCCT CAGAAGTTTC TAGAAACAGA 
GGAGCCTCTT TACTGGCCAG GCCAGGACAC AGCTTAGTAG CTGCTATTCG AAGCCAACAT 
CAACATGAGT CATTGCCACT GGCATATAAT GACAAGATTG TGGCATTTCT TCGCCAGCCA 
AACATTTTTG AAATGCTGCA AGAGCGTCAG CCAAGCTTAG CAAGAAACCA CACACTCAGG 
GAGAAAATCC ATTACATTCG GACTGAGGGT AATCACGGGC TTGAGAAGTT GTCCTGTGAT 
GCGGATCTGG TCATTTTGCT GAGTCTCTTT GAAGAAGAGA TTATGTCCTA CGTCCCCCTG 
CAGGCTGCCT TCCACCCTGG GTATAGCTTC TCTCCCCGAT GTTCACCCTG TTCTTCACCT 
CAGAACTCCC CAGGTTTACA GAGAGCCAGT GCAAGAGCCC CTTCCCCCTA CCGAAGAGAC 
TTTGAGGCCA AGCTCCGCAA TTTCTACAGA AAACTGGAAG CCAAAGGATT TGGTCAGGGT 
CCGGGGAAAA TTAAGCTCAT TATTCGCCGG GATCATTTGT TGGAGGGAAC CTTCAATCAG 
GTGATGGCCT ATTCGCGGAA AGAGCTCCAG CGAAACAAGC TCTACGTCAC CTTTGTTGGA 
GAGGAGGGCC TGGACTACAG TGGCCCCTCG CGGGAGTTCT TCTTCCTTCT GTCTCAGGAG 
CTCTTCAACC CTTACTATGG ACTCTTTGAG TACTCGGCAA ATGATACTTA CACGGTGCAG 
ATCAGCCCCA TGTCCGCATT TGTAGAAAAC CATCTTGAGT GGTTCAGGTT TAGCGGTCGC 
ATCCTGGGTC TGGCTCTGAT CCATCAGTAC CTTCTTGACG CTTTCTTCAC GAGGCCCTTC 
TACAAGGCAC TCCTGAGACT GCCCTGTGAT TTGAGTGACC TGGAATATTT GGATGAGGAA 
TTCCACCAGA GTTTGCAGTG GATGAAGGAC AACAACATCA CAGACATCTT AGACCTCACT 
TTCACTGTTA ATGAAGAGGT TTTTGGACAG GTCACGGAAA GGGAGTTGAA GTCTGGAGGA 
GCCAACACAC AGGTGACGGA GAAAAACAAG AAGGAGTACA TCGAGCGCAT GGTGAAGTGG 
CGGGTGGAGC GCGGCGTGGT ACAGCAGACC GAGGCGCTGG TGCGCGGCTT CTACGAGGTT 
GTAGACTCGA GGCTGGTGTC CGTGTTTGAT GCCAGGGAGC TGGAGCTGGT GATAGCTGGC 
ACCGCGGAAA TCGACCTAAA TGACTGGCGG AATAACACTG AGTACCGGGG AGGTTACCAC 
GATGGGCATC TTGTGATCCG CTGGTTCTGG GCTGCGGTGG AGCGCTTCAA TAATGAGCAG 
AGGCTGAGAT TACTGCAGTT TGTCACGGGA ACATCCAGCG TGCCCTACGA AGGCTTCGCA 
GCCCTCCGTG GGAGCAATGG GCTTCGGCGC TTCTGCATAG AGAAATGGGG GAAAATTACT 
TCTCTCCCCA GGGCACACAC ATGCTTCAAC CGACTGGATC TTCCACCGTA TCCCTCGTAC 
TCCATGTTGT ATGAAAAGCT GTTAACAGCA GTAGAGGAAA CCAGCACCTT TGGACTTGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_017011890.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867379.1 Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X19, mRNA.

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CloneID	OHu97119
Clone ID Related Accession (Same CDS sequence)	XM_017011890.1
Accession Version	XM_017011890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase HECW1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT ACATGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGATGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAGTTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGC 
CCGCAGGTCC ATGACACTCA GGAAACCAGA ATCCTGAGTC CTCAAATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867379.1
CDS	390..4460
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYNPDQFHNMDLRGGPHDGVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGADETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEISLSTEPESAQIQDSPMNNLMESGSGEPRSEAPESSESWKPEQLGEGSVPDGPGNQSIELSRPAEEAAVITEAGDQGMVSVGPEGAGELLAQVQKDIQPAPSAEELAEQLDLGEEASALLLEDGEAPASTKEEPLEEEATTQSRAGREEEEKEQEEEGDVSTLEQGEGRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGSAAEEEDGAEEESTLKDSSEKDGLSEVDTVAADPSALEEDREEPEGATPGTAHPGHSGGHFPSLANGAAQDGDTHPSTGSESDSSPRQGGDHSCEGCDASCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGNRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSMQSPELDPESTNGAGPWQDELAAPSGHVERSPEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERSEEDSGSQSCEQAPAGGGGGGGSDSEAESSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYSAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQAAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLECPQVHDTQETRILSPQI

Target ORF information:

RefSeq Version	XM_017011890.1
Organism	Homo sapiens(human)
Definition	Homo sapiens HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011890.1

ORF Insert Sequence:

ATGCTGCTGC ACCTGTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCGT CTCCTTCTAG AAACTCCCAG AGCCGACGCC GGTGCAAGGA GCCGCTCCGA 
TACAGCTACA ACCCCGACCA GTTCCACAAC ATGGACCTCA GGGGCGGCCC CCACGATGGC 
GTCACCATTC CCCGCTCCAC CAGCGACACT GACCTGGTCA CCTCGGACAG CCGCTCCACG 
CTCATGGTCA GCAGCTCCTA CTATTCCATC GGGCACTCTC AGGACCTGGT CATCCACTGG 
GACATAAAGG AGGAAGTGGA CGCTGGGGAC TGGATTGGCA TGTACCTCAT TGATGAGGTC 
TTGTCCGAAA ACTTTCTGGA CTATAAAAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATCGATGC CAGCTCGTAC TTTGTGGAAC CTGAAACTAA GATCTGCTTC 
AAATACTACC ATGGAGTGAG TGGGGCCCTG CGAGCAACCA CCCCCAGTGT CACGGTCAAA 
AACTCGGCAG CTCCTATTTT TAAAAGCATT GGTGCTGATG AGACCGTCCA AGGACAAGGA 
AGTCGGAGGC TGATCAGCTT CTCTCTCTCA GATTTCCAAG CCATGGGGTT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTATCTGAAG ATTTCCATTC AGCCTGGGAA ACACAGCATC 
TTCCCCGCCC TCCCTCACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACCGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGT TTTGTGTCCT TGCCCACTGA CGTGCTGGAA 
ATTGAGGTGA AGGACAAGTT TGCCAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TACACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CCTCCATCCA CCCAGATGAT GAGGAGATTT CCCTGAGTAC CGAGCCTGAG 
TCAGCCCAAA TTCAGGACAG CCCCATGAAC AACCTGATGG AAAGCGGCAG TGGGGAACCT 
CGGTCTGAGG CACCAGAGTC CTCTGAGAGC TGGAAGCCAG AGCAGCTGGG TGAGGGCAGT 
GTCCCCGATG GTCCAGGGAA CCAAAGCATA GAGCTTTCCA GACCAGCTGA GGAAGCAGCA 
GTCATCACGG AGGCAGGAGA CCAGGGCATG GTCTCTGTGG GACCTGAAGG GGCTGGGGAG 
CTCCTGGCCC AGGTGCAAAA GGACATCCAG CCTGCCCCCA GTGCAGAAGA GCTGGCCGAG 
CAGCTGGACC TGGGTGAGGA GGCATCAGCA CTGCTGCTGG AAGACGGTGA AGCCCCAGCC 
AGCACCAAGG AGGAGCCCTT GGAGGAGGAA GCAACGACCC AGAGCCGGGC TGGAAGGGAA 
GAAGAGGAGA AGGAGCAGGA GGAGGAGGGA GATGTGTCTA CCCTGGAGCA GGGAGAGGGC 
AGGCTGCAGC TGCGGGCCTC GGTGAAGAGA AAAAGCAGGC CCTGCTCCTT GCCTGTGTCC 
GAGCTGGAGA CGGTGATCGC GTCAGCCTGC GGGGACCCCG AGACCCCGCG GACACACTAC 
ATCCGCATCC ACACCCTGCT GCACAGCATG CCCTCCGCCC AGGGCGGCAG CGCGGCAGAG 
GAGGAGGACG GCGCGGAGGA GGAGTCCACC CTCAAGGACT CCTCGGAGAA GGATGGGCTC 
AGCGAGGTGG ACACGGTGGC CGCTGACCCG TCTGCCCTGG AAGAGGACAG AGAAGAGCCC 
GAGGGGGCTA CTCCAGGCAC GGCGCACCCT GGCCACTCCG GGGGCCACTT CCCCAGCCTG 
GCCAATGGCG CGGCCCAGGA TGGCGACACG CACCCCAGCA CCGGGAGCGA GAGCGACTCC 
AGCCCCAGGC AAGGCGGGGA CCACAGTTGC GAGGGCTGTG ACGCGTCCTG CTGCAGCCCC 
TCGTGCTACA GCTCCTCGTG CTACAGCACG TCCTGCTACA GCAGCTCGTG CTACAGCGCC 
TCGTGCTACA GCCCCTCCTG CTACAACGGC AACAGGTTCG CCAGCCACAC GCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCCGAGAGC ACGGTCTTCT CCTCGCAAGA CGACGAGGAG 
GAGGAGAACA GCGCGTTCGA GTCGGTACCC GACTCCATGC AGAGCCCTGA GCTGGACCCG 
GAGTCCACGA ACGGCGCTGG GCCGTGGCAA GACGAGCTGG CCGCCCCTAG CGGGCACGTG 
GAAAGAAGCC CGGAAGGTCT GGAATCCCCC GTGGCAGGTC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCCT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGAATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CAACCACCTG GCAGCGTCCG 
ACGGCAGCAG CCACCCCGGA TGGCATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA CATTCAGCGA ACCATTGCAA CAGAGAGGTC CGAAGAAGAT 
TCTGGCAGCC AAAGCTGCGA GCAAGCCCCA GCAGGAGGAG GCGGAGGTGG AGGGAGTGAC 
TCAGAAGCCG AATCTTCCCA GTCCAGCTTA GATCTAAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGCTGCTG CAGTCCCCAG CGGTCAAGTT CATCACCAAC 
CCCGAGTTCT TCACTGTGCT ACATGCCAAT TATAGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGACGGGATG CTCGCAATTT TGAACGCTAC 
CAGCACAACC GGGACTTGGT GAATTTCATC AACATGTTCG CAGACACTCG GCTGGAACTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAC 
AGTCGAGCTA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CATCTAACTC ACCGACAGCA CCTCCAGAGG CTCCGAAGTT ACAGCGCTGG AGAGGCCTCA 
GAAGTTTCTA GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CTTAGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTGG CATATAATGA CAAGATTGTG 
GCATTTCTTC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCTTAGCA 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCACGGGCTT 
GAGAAGTTGT CCTGTGATGC GGATCTGGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCCCTGCA GGCTGCCTTC CACCCTGGGT ATAGCTTCTC TCCCCGATGT 
TCACCCTGTT CTTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCCCCCTACC GAAGAGACTT TGAGGCCAAG CTCCGCAATT TCTACAGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC GGGGAAAATT AAGCTCATTA TTCGCCGGGA TCATTTGTTG 
GAGGGAACCT TCAATCAGGT GATGGCCTAT TCGCGGAAAG AGCTCCAGCG AAACAAGCTC 
TACGTCACCT TTGTTGGAGA GGAGGGCCTG GACTACAGTG GCCCCTCGCG GGAGTTCTTC 
TTCCTTCTGT CTCAGGAGCT CTTCAACCCT TACTATGGAC TCTTTGAGTA CTCGGCAAAT 
GATACTTACA CGGTGCAGAT CAGCCCCATG TCCGCATTTG TAGAAAACCA TCTTGAGTGC 
CCGCAGGTCC ATGACACTCA GGAAACCAGA ATCCTGAGTC CTCAAATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.
The Journal of cell biology212(4)409-23(2016 Feb)
Inaba H,Goto H,Kasahara K,Kumamoto K,Yonemura S,Inoko A,Yamano S,Wanibuchi H,He D,Goshima N,Kiyono T,Hirotsune S,Inagaki M

Meta-analysis of genome-wide association studies in African Americans provides insights into the genetic architecture of type 2 diabetes.
PLoS genetics10(8)e1004517(2014 Aug)
Ng MC,Shriner D,Chen BH,Li J,Chen WM,Guo X,Liu J,Bielinski SJ,Yanek LR,Nalls MA,Comeau ME,Rasmussen-Torvik LJ,Jensen RA,Evans DS,Sun YV,An P,Patel SR,Lu Y,Long J,Armstrong LL,Wagenknecht L,Yang L,Snively BM,Palmer ND,Mudgal P,Langefeld CD,Keene KL,Freedman BI,Mychaleckyj JC,Nayak U,Raffel LJ,Goodarzi MO,Chen YD,Taylor HA Jr,Correa A,Sims M,Couper D,Pankow JS,Boerwinkle E,Adeyemo A,Doumatey A,Chen G,Mathias RA,Vaidya D,Singleton AB,Zonderman AB,Igo RP Jr,Sedor JR,,Kabagambe EK,Siscovick DS,McKnight B,Rice K,Liu Y,Hsueh WC,Zhao W,Bielak LF,Kraja A,Province MA,Bottinger EP,Gottesman O,Cai Q,Zheng W,Blot WJ,Lowe WL,Pacheco JA,Crawford DC,,,Grundberg E,,Rich SS,Hayes MG,Shu XO,Loos RJ,Borecki IB,Peyser PA,Cummings SR,Psaty BM,Fornage M,Iyengar SK,Evans MK,Becker DM,Kao WH,Wilson JG,Rotter JI,Sale MM,Liu S,Rotimi CN,Bowden DW,

Genome-wide association study of the rate of cognitive decline in Alzheimer's disease.
Alzheimer's & dementia : the journal of the Alzheimer's Association10(1)45-52(2014 Jan)
Sherva R,Tripodis Y,Bennett DA,Chibnik LB,Crane PK,de Jager PL,Farrer LA,Saykin AJ,Shulman JM,Naj A,Green RC,,,

A genome-wide association study identifies new susceptibility loci for esophageal adenocarcinoma and Barrett's esophagus.
Nature genetics45(12)1487-93(2013 Dec)
Levine DM,Ek WE,Zhang R,Liu X,Onstad L,Sather C,Lao-Sirieix P,Gammon MD,Corley DA,Shaheen NJ,Bird NC,Hardie LJ,Murray LJ,Reid BJ,Chow WH,Risch HA,Nyr?n O,Ye W,Liu G,Romero Y,Bernstein L,Wu AH,Casson AG,Chanock SJ,Harrington P,Caldas I,Debiram-Beecham I,Caldas C,Hayward NK,Pharoah PD,Fitzgerald RC,Macgregor S,Whiteman DC,Vaughan TL

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HECW1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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