hecw1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hecw1b
Entrez Gene ID	563730
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b
Synonyms	hecw1,si:ch211-106n13.1
General protein information	Preferred Names HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b Names E3 ubiquitin-protein ligase HECW1 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 nedl1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009298865.3	XP_009297140.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_005168424.3	XP_005168481.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_009298865.2	XP_009297140.1	E3 ubiquitin-protein ligase HECW1 isoform X1
NM_001145764.1	NP_001139236.1	E3 ubiquitin-protein ligase HECW1
XM_021480874.1	XP_021336549.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_021480876.1	XP_021336551.1	E3 ubiquitin-protein ligase HECW1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	HECW1	NP_055867.3
Pan troglodytes (chimpanzee)	HECW1	XP_003318432.1
Canis lupus familiaris (dog)	HECW1	XP_005630981.1
Danio rerio (zebrafish)	hecw1b	NP_001139236.1
Mus musculus (house mouse)	Hecw1	NP_001074817.3
Xenopus tropicalis (tropical clawed frog)	hecw1	NP_001123750.1
	HECW1	XP_001096775.1
Rattus norvegicus (Norway rat)	Hecw1	NP_001099587.1
Gallus gallus (chicken)	HECW1	XP_004939648.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Sex differences in DNA methylation and expression in zebrafish brain: a test of an extended 'male sex drive' hypothesis.
Chatterjee A, Lagisz M, Rodger EJ, Zhen L, Stockwell PA, Duncan EJ, Horsfield JA, Jeyakani J, Mathavan S, Ozaki Y, Nakagawa S
Gene590(2)307-16(2016 Sep)

Characterization of ?? T Cells from Zebrafish Provides Insights into Their Important Role in Adaptive Humoral Immunity.
Wan F, Hu CB, Ma JX, Gao K, Xiang LX, Shao JZ
Frontiers in immunology7675(2016)

Itch is required for lateral line development in zebrafish.
Angers A, Drapeau P
PloS one9(11)e111799(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hecw1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hecw1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa150354	XM_021480874.1 Latest version!	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa150355	XM_021480876.1 Latest version!	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa35770	NM_001145764.1 Latest version!	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa07503	XM_005168424.3 Latest version!	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa44163	XM_009298865.3 Latest version!	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa44163	XM_009298865.2	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hecw1b ( XM_021480874.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336549.1 Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa150354
Clone ID Related Accession (Same CDS sequence)	XM_021480874.1
Accession Version	XM_021480874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGAGG AGGTGATGAT TCACCGCCAG GTGATACTGA CCCAGAACTT GTACCAGAGC 
AGGTTTCTCG GGCTTGCAGC TATGGCCTCT CCATCTCGTG CATCTCAGGG CAGGAGGCGA 
TGTAAGGACC CGATCCGTCA CAGTTTTAAC CCTGAGCAGT TCCCTAATGT GGACTTCCAG 
AACGGCGTGG CGGAGGATCT GGCGGCGGCC GTGCTGCGCT CTACCAGCGA CACTGACCTG 
CTGACATCCC ACTGCCGCTC CACCCTCACC GTTAGCACCT CCAGCTACAC CATCGGTCAG 
ACGCAGGACA TCACCCTCAG CTGGGACATC AAGGAGGAGG TGGACGCCGG AGACTGGATT 
GGCATGTACC TCATTGATGA GGTTCTGTCA GAGAATTTCC TGGACTACAA AAACCGGGGT 
GTGAACGGCT CGCACAAAGG GCAGATTATC TGGAAGATTG AGGCCAGCTC CTATTTTGTG 
GAACCGGAGA CTAAGATCTG TTTTAAATAT TATCATGGAG TTAGTGGAGC GCTTCGGGCC 
ACAACACCAT GCGTGACTGT GAAGAACCCC TCTGCACCGG TGTTTAAGCC GGTGGCCACA 
GACGATGTTC CTCAGAGTCA GGGAAGAAGG AGACTCATCA GCTTCTCTCT GTCAGATTTC 
CAGGCCACCG GACTGAAAAA AGGCATGTTT TTTAACCCCG ACCCCTACCT AAAGATCGCT 
ATCCACCCTG GCAAACACAG CATCTTCCCA GCGCTGCCTC ACCATGGCCA GGAGAAGAGA 
TCCGGCATCG TCTGCAATAC CATTAATCCT GTTTGGCAGA GGGAGCGCTT TAATTTTGTG 
TCTCTGCCTA CGGATGTGTT GGAGATTGAG GTGAAGGACA AGTTCGCCAA AAGCCGTCCC 
ATCATAAAGC GTTTCTTGGG CAAGCTGTCC ATGCCAGTGC AAAGACTACT GGAGAAGCAT 
GCTATCGGTG ATCGAGTGGT CAGCTATACT CTGGGTCGAA GACTTCCAAC AGAGCATGTC 
AGCGGGCAGC TGCAGTTCAG GTTCGAGATC ACCTCCTCCA TTCATCCAGA TGATGAAGAC 
ATGTCTCTCA GCACAGAGCA GCCGGTGGCA GCAGCAGAAG CTCAAGCTCA GCCTCAAGCT 
GAAGACACCA TGAGTTTAGA TCTCGGAGCC CCGGAGCTGC CCTTAGATGG AGATACGGCT 
TCAGTCGAGA TGCCCATGGA TGTTGAATCT GGTCAGAGTT GTGCAGCTGA AGCAGATGTC 
ATAGAGGAAG AGTGTTTAGA GGAAGAACAG CAGGAAGGAG AAGCAGCAGC AGCAGCAGCA 
GCAATGGAGC AAAGTGAAAC CAATGCAGAA GAAACAACAC AAGTCTTGGA TCCCATCAAT 
GGCGCTGAAG AAGAAGAAGC AGAGAATAAT TGCGCCTTTG CAGAAGAGTC TTCAGATGCT 
CGCGCAGGAG AGGAAGATGA AGCGATTTCA GATCCGCAGC CAGCTTCAGA AGAGCCGGAT 
GCGGATTTAG AGGAAGACTG TTCACTGAGG ATCCGAACCA CTCCAAGAAG GAAACCCCGT 
CCCTGCTCTT TACCTGTCTC TGAGCTTGAG ACGGTCATTG CTTCAGCCTG TGGGGAACCT 
GAAACGCCAC GCTCTCATTA CATCCGAATA CACCACCTGC TCCACAGCCT GCCGGCCACT 
CAACCGCAGA CAGGAGACTC CGCCGGGCCT GAACCAGACC GACCAATGGG TGTAGAGGAG 
GAAGATGAAG AGGAAGAAGA AGAAGGTGTA ACTCTTGAAG ATCATGCTTT AACACCCAAT 
GGCCCTCGCA GGACTCTTCC TCGCAGCCGC TCTCACGAAC GCCTCTCTGA CCTCATTCAG 
ATGCTGGATG GAGATGGACA TCCGCTTGGG GCTGAGGGGC AAACCGGAAA TCAGAGGTCA 
CCTGATTTGA GCCCAGTGGC GTGCTGCAGT CACATGTCCC CGAGAGCTTC TGGTCCCATT 
CGGGCACAAT CCCTAGACAG TGCGCGGAGG TCAGAGAGTA CGGTGTTCTC CTCGCAGGAT 
GACGAGGACG AGATGGAGCA GCCAAATGGC ATTCTGGGAT CAGAGACGGA AACAGAGCAG 
GGCGCTCAAT GTCGGGAGCC AGGCGACGGG AGCTGTCAGT GGGAGGAGGC ACCGGACAGT 
CACGTTCAGA GCCCTCATGG CATTGTGGGT AATGGAGAAG TGGCCGGACC GAGCGGCAGA 
CGAGAATGTC CTTTGCCTTG TAACCACCCT GCAGTGAGTC AGCTGCCTGC CCTGCGGCCC 
GATCCACACC ACTATCCCAC CATTGATGAG CCGCTGCCGC CCAACTGGGA GGCCCGGATC 
GACAGTCACG GTCGTGTGTT TTATGTGGAT CACATTAACC GGACCACCAC TTGGCAAAGA 
CCCACATCTG CAGCCACGCC GGACGGCCTA CGCAGGTCTG GATCCGTCCA ACAGATGGAG 
CAGCTCAACA GGAGGTATCA GACAATCCAG AGGACGATGG CCACCGAGAG GAGAGAAGAG 
GAATCAGGAA GCCCCCGTAA TGACAGAACA GCCAATACTG AGACAGACTC ACCTCTGCAA 
ACAAACGAGT TGAGGAGAGA CACCTCATGT TCTCCAGGCC ACTGTCAGAA GATTACGTTA 
CTGTTACAGA GTCCAGCGGT CAAATTCATC ACTCACCCAG AGTTCTTCAC TGTTCTCCAC 
GCTAATTATG GTGCATATCG AATGTTCACC AACAGCTCGT GTCTGAAACA CATGGTCCTG 
AAAATTCGAC GAGATGCTCG AAATTTTGAG CGCTACCAGC ACAATCGTGA CCTGGTGACG 
TTTCTGAACA GGTTTGCAGA CGCTCAGCTG GAGCTGCCGC GCGGCTGGGA GATCAAAACT 
GACCCGCAGG GGAAGTCATT TTTTGTAGAC CACAACAGTC GTGCCACAAC CTTCATCGAT 
CCCAGAATCC CCTTGCAAAA CGGCAGATTA CCAAACCACC TGACGCACCG CCAGCACCTG 
CAGAGATTAC GCAGCTACAG CGCAGGAGAG GCATCTGAGG TGTCGCGCAC CCGAGGGGTG 
TCTTTGTTAG CCAGGCCTGG AAACAGCCTG GTAGCAGCCA TACGAAACCA AAGCCAGCAG 
GAGCCTGTGC CATTAGCGTA CAATGATAAA ATTGTAGCAT TTTTACGGCA ACCAAACATC 
TTCGAGGTGC TACAGGAGCG TCAGCCCAGC TTTGCCAGGA ATCACTCACT CAAGGATAAA 
GTGCATCACA TCCGAACCGA AGGCACACAG AGACTGGAGA AGCTGTCATG TGATGCTGAC 
CTGGTCATGC TGTTAAGTTT GTTTGAGGAG GACATCATGT CTTACGTTCC TCTCGCATCA 
TTTCACCCCG GGTTCAACTT TTCACCTCGC TGCTCTCCTG GTTCTTCTCC CCAGAACTCT 
CCAGGCTTTC TGGAGCGAAC TCCTCTCGTT CCAGCCTGCT GTCTGTCTGG TGAGAGCTCT 
CCAGGGACCC AGAGGGCTCG GGCTCCAGCG CCGTACAGAA GAGACTTTGA GGCCAAATTG 
CGCAACTTCT ACAGGAAGCT TGAGGCAAAG GGCTACGGGC AAGGGCCTGG GAAGATAAAA 
TTGATCGTCA GAAGAGATCA TCTTTTAGAA GGAACGTTTA ACCAGGTCAT GGCCTACTCA 
CGTAAAGAGC TGCAGAGAAA CAAGCTGTAC ATTACCTTTG TGGGAGAGGA AGGTCTGGAC 
TACAGTGGCC CATCGCGAGA GTTCTTCTTC CTGCTGTCTC AGGAGCTGTT TAACCCTTAC 
TATGGCCTGT TTGAATACTC CGCCAACGAC ACATACACAG TCCAGATCAG CCCCATGTCT 
GCTTTTGTGG AGAACCACTT GGAATGGTTC CGCTTCAGTG GTCGAATTCT CGGCCTGGCT 
TTGATTCATC AGTATTTGTT GGACGCTTTC TTCACTCGGC CATTTTATAA AGCTCTTCTC 
AGACTACCCA CTGATCTCAG CGATCTGGAG TATCTGGATG AGGAGTTTCA CCAGAGCTTG 
CAGTGGATGA AGGAAAATGA CATCACTGAT GTGCTGGACC TCACCTTCAC TGTCAATGAG 
GAAGTGTTCG GACAGGTGAC AGAGCGGGAG CTGAAGTCTG GCGGGACGAA TGTGCAGGTG 
ACGGAGAAAA ATAAGAAGGA GTACATTGAG CGGATGGTGA AGTGGAGGGT GGAGCGAGGC 
GTTGTCCAGC AGACACAGGC TCTGGTGCGA GGATTTTATG AGGTGGTGGA CTCTCGTCTG 
GTGTCTGTGT TTGATGCCAG AGAGCTGGAG CTGGTCATCG CAGGAACAGC AGAGATCGAT 
TTGAACGACT GGAGAAACAA CACAGAGTAC AGAGGAGGTT ATCATGACGG GCACATTGTG 
ATCCGCTGGT TCTGGGGAGC AGTGGAGCGC TTCAATAATG AGCAGCGGCT GCGTCTGCTG 
CAGTTTGTGA CGGGCACCTC CAGCGTGCCG TATGAGGGCT TTACCGCCCT ACGTGGCAGC 
AATGGACTGA GACGCTTCTG CATTGAGAAA TGGGGAAAGA TCACATCACT CCCAAGGGCG 
CACACATGCT TTAACCGTCT GGATCTTCCA CCATATCCAT CCTACACGAT GTTATACGAG 
AAACTCCTGA TCGCTGTGGA AGAGACCAGC ACTTTTGGCC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336549.1
CDS	152..4819
Translation	MNEEVMIHRQVILTQNLYQSRFLGLAAMASPSRASQGRRRCKDPIRHSFNPEQFPNVDFQNGVAEDLAAAVLRSTSDTDLLTSHCRSTLTVSTSSYTIGQTQDITLSWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHKGQIIWKIEASSYFVEPETKICFKYYHGVSGALRATTPCVTVKNPSAPVFKPVATDDVPQSQGRRRLISFSLSDFQATGLKKGMFFNPDPYLKIAIHPGKHSIFPALPHHGQEKRSGIVCNTINPVWQRERFNFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLEKHAIGDRVVSYTLGRRLPTEHVSGQLQFRFEITSSIHPDDEDMSLSTEQPVAAAEAQAQPQAEDTMSLDLGAPELPLDGDTASVEMPMDVESGQSCAAEADVIEEECLEEEQQEGEAAAAAAAMEQSETNAEETTQVLDPINGAEEEEAENNCAFAEESSDARAGEEDEAISDPQPASEEPDADLEEDCSLRIRTTPRRKPRPCSLPVSELETVIASACGEPETPRSHYIRIHHLLHSLPATQPQTGDSAGPEPDRPMGVEEEDEEEEEEGVTLEDHALTPNGPRRTLPRSRSHERLSDLIQMLDGDGHPLGAEGQTGNQRSPDLSPVACCSHMSPRASGPIRAQSLDSARRSESTVFSSQDDEDEMEQPNGILGSETETEQGAQCREPGDGSCQWEEAPDSHVQSPHGIVGNGEVAGPSGRRECPLPCNHPAVSQLPALRPDPHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPTSAATPDGLRRSGSVQQMEQLNRRYQTIQRTMATERREEESGSPRNDRTANTETDSPLQTNELRRDTSCSPGHCQKITLLLQSPAVKFITHPEFFTVLHANYGAYRMFTNSSCLKHMVLKIRRDARNFERYQHNRDLVTFLNRFADAQLELPRGWEIKTDPQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRTRGVSLLARPGNSLVAAIRNQSQQEPVPLAYNDKIVAFLRQPNIFEVLQERQPSFARNHSLKDKVHHIRTEGTQRLEKLSCDADLVMLLSLFEEDIMSYVPLASFHPGFNFSPRCSPGSSPQNSPGFLERTPLVPACCLSGESSPGTQRARAPAPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIVRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPTDLSDLEYLDEEFHQSLQWMKENDITDVLDLTFTVNEEVFGQVTERELKSGGTNVQVTEKNKKEYIERMVKWRVERGVVQQTQALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWGAVERFNNEQRLRLLQFVTGTSSVPYEGFTALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYTMLYEKLLIAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_021480874.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480874.1

ORF Insert Sequence:

ATGAACGAGG AGGTGATGAT TCACCGCCAG GTGATACTGA CCCAGAACTT GTACCAGAGC 
AGGTTTCTCG GGCTTGCAGC TATGGCCTCT CCATCTCGTG CATCTCAGGG CAGGAGGCGA 
TGTAAGGACC CGATCCGTCA CAGTTTTAAC CCTGAGCAGT TCCCTAATGT GGACTTCCAG 
AACGGCGTGG CGGAGGATCT GGCGGCGGCC GTGCTGCGCT CTACCAGCGA CACTGACCTG 
CTGACATCCC ACTGCCGCTC CACCCTCACC GTTAGCACCT CCAGCTACAC CATCGGTCAG 
ACGCAGGACA TCACCCTCAG CTGGGACATC AAGGAGGAGG TGGACGCCGG AGACTGGATT 
GGCATGTACC TCATTGATGA GGTTCTGTCA GAGAATTTCC TGGACTACAA AAACCGGGGT 
GTGAACGGCT CGCACAAAGG GCAGATTATC TGGAAGATTG AGGCCAGCTC CTATTTTGTG 
GAACCGGAGA CTAAGATCTG TTTTAAATAT TATCATGGAG TTAGTGGAGC GCTTCGGGCC 
ACAACACCAT GCGTGACTGT GAAGAACCCC TCTGCACCGG TGTTTAAGCC GGTGGCCACA 
GACGATGTTC CTCAGAGTCA GGGAAGAAGG AGACTCATCA GCTTCTCTCT GTCAGATTTC 
CAGGCCACCG GACTGAAAAA AGGCATGTTT TTTAACCCCG ACCCCTACCT AAAGATCGCT 
ATCCACCCTG GCAAACACAG CATCTTCCCA GCGCTGCCTC ACCATGGCCA GGAGAAGAGA 
TCCGGCATCG TCTGCAATAC CATTAATCCT GTTTGGCAGA GGGAGCGCTT TAATTTTGTG 
TCTCTGCCTA CGGATGTGTT GGAGATTGAG GTGAAGGACA AGTTCGCCAA AAGCCGTCCC 
ATCATAAAGC GTTTCTTGGG CAAGCTGTCC ATGCCAGTGC AAAGACTACT GGAGAAGCAT 
GCTATCGGTG ATCGAGTGGT CAGCTATACT CTGGGTCGAA GACTTCCAAC AGAGCATGTC 
AGCGGGCAGC TGCAGTTCAG GTTCGAGATC ACCTCCTCCA TTCATCCAGA TGATGAAGAC 
ATGTCTCTCA GCACAGAGCA GCCGGTGGCA GCAGCAGAAG CTCAAGCTCA GCCTCAAGCT 
GAAGACACCA TGAGTTTAGA TCTCGGAGCC CCGGAGCTGC CCTTAGATGG AGATACGGCT 
TCAGTCGAGA TGCCCATGGA TGTTGAATCT GGTCAGAGTT GTGCAGCTGA AGCAGATGTC 
ATAGAGGAAG AGTGTTTAGA GGAAGAACAG CAGGAAGGAG AAGCAGCAGC AGCAGCAGCA 
GCAATGGAGC AAAGTGAAAC CAATGCAGAA GAAACAACAC AAGTCTTGGA TCCCATCAAT 
GGCGCTGAAG AAGAAGAAGC AGAGAATAAT TGCGCCTTTG CAGAAGAGTC TTCAGATGCT 
CGCGCAGGAG AGGAAGATGA AGCGATTTCA GATCCGCAGC CAGCTTCAGA AGAGCCGGAT 
GCGGATTTAG AGGAAGACTG TTCACTGAGG ATCCGAACCA CTCCAAGAAG GAAACCCCGT 
CCCTGCTCTT TACCTGTCTC TGAGCTTGAG ACGGTCATTG CTTCAGCCTG TGGGGAACCT 
GAAACGCCAC GCTCTCATTA CATCCGAATA CACCACCTGC TCCACAGCCT GCCGGCCACT 
CAACCGCAGA CAGGAGACTC CGCCGGGCCT GAACCAGACC GACCAATGGG TGTAGAGGAG 
GAAGATGAAG AGGAAGAAGA AGAAGGTGTA ACTCTTGAAG ATCATGCTTT AACACCCAAT 
GGCCCTCGCA GGACTCTTCC TCGCAGCCGC TCTCACGAAC GCCTCTCTGA CCTCATTCAG 
ATGCTGGATG GAGATGGACA TCCGCTTGGG GCTGAGGGGC AAACCGGAAA TCAGAGGTCA 
CCTGATTTGA GCCCAGTGGC GTGCTGCAGT CACATGTCCC CGAGAGCTTC TGGTCCCATT 
CGGGCACAAT CCCTAGACAG TGCGCGGAGG TCAGAGAGTA CGGTGTTCTC CTCGCAGGAT 
GACGAGGACG AGATGGAGCA GCCAAATGGC ATTCTGGGAT CAGAGACGGA AACAGAGCAG 
GGCGCTCAAT GTCGGGAGCC AGGCGACGGG AGCTGTCAGT GGGAGGAGGC ACCGGACAGT 
CACGTTCAGA GCCCTCATGG CATTGTGGGT AATGGAGAAG TGGCCGGACC GAGCGGCAGA 
CGAGAATGTC CTTTGCCTTG TAACCACCCT GCAGTGAGTC AGCTGCCTGC CCTGCGGCCC 
GATCCACACC ACTATCCCAC CATTGATGAG CCGCTGCCGC CCAACTGGGA GGCCCGGATC 
GACAGTCACG GTCGTGTGTT TTATGTGGAT CACATTAACC GGACCACCAC TTGGCAAAGA 
CCCACATCTG CAGCCACGCC GGACGGCCTA CGCAGGTCTG GATCCGTCCA ACAGATGGAG 
CAGCTCAACA GGAGGTATCA GACAATCCAG AGGACGATGG CCACCGAGAG GAGAGAAGAG 
GAATCAGGAA GCCCCCGTAA TGACAGAACA GCCAATACTG AGACAGACTC ACCTCTGCAA 
ACAAACGAGT TGAGGAGAGA CACCTCATGT TCTCCAGGCC ACTGTCAGAA GATTACGTTA 
CTGTTACAGA GTCCAGCGGT CAAATTCATC ACTCACCCAG AGTTCTTCAC TGTTCTCCAC 
GCTAATTATG GTGCATATCG AATGTTCACC AACAGCTCGT GTCTGAAACA CATGGTCCTG 
AAAATTCGAC GAGATGCTCG AAATTTTGAG CGCTACCAGC ACAATCGTGA CCTGGTGACG 
TTTCTGAACA GGTTTGCAGA CGCTCAGCTG GAGCTGCCGC GCGGCTGGGA GATCAAAACT 
GACCCGCAGG GGAAGTCATT TTTTGTAGAC CACAACAGTC GTGCCACAAC CTTCATCGAT 
CCCAGAATCC CCTTGCAAAA CGGCAGATTA CCAAACCACC TGACGCACCG CCAGCACCTG 
CAGAGATTAC GCAGCTACAG CGCAGGAGAG GCATCTGAGG TGTCGCGCAC CCGAGGGGTG 
TCTTTGTTAG CCAGGCCTGG AAACAGCCTG GTAGCAGCCA TACGAAACCA AAGCCAGCAG 
GAGCCTGTGC CATTAGCGTA CAATGATAAA ATTGTAGCAT TTTTACGGCA ACCAAACATC 
TTCGAGGTGC TACAGGAGCG TCAGCCCAGC TTTGCCAGGA ATCACTCACT CAAGGATAAA 
GTGCATCACA TCCGAACCGA AGGCACACAG AGACTGGAGA AGCTGTCATG TGATGCTGAC 
CTGGTCATGC TGTTAAGTTT GTTTGAGGAG GACATCATGT CTTACGTTCC TCTCGCATCA 
TTTCACCCCG GGTTCAACTT TTCACCTCGC TGCTCTCCTG GTTCTTCTCC CCAGAACTCT 
CCAGGCTTTC TGGAGCGAAC TCCTCTCGTT CCAGCCTGCT GTCTGTCTGG TGAGAGCTCT 
CCAGGGACCC AGAGGGCTCG GGCTCCAGCG CCGTACAGAA GAGACTTTGA GGCCAAATTG 
CGCAACTTCT ACAGGAAGCT TGAGGCAAAG GGCTACGGGC AAGGGCCTGG GAAGATAAAA 
TTGATCGTCA GAAGAGATCA TCTTTTAGAA GGAACGTTTA ACCAGGTCAT GGCCTACTCA 
CGTAAAGAGC TGCAGAGAAA CAAGCTGTAC ATTACCTTTG TGGGAGAGGA AGGTCTGGAC 
TACAGTGGCC CATCGCGAGA GTTCTTCTTC CTGCTGTCTC AGGAGCTGTT TAACCCTTAC 
TATGGCCTGT TTGAATACTC CGCCAACGAC ACATACACAG TCCAGATCAG CCCCATGTCT 
GCTTTTGTGG AGAACCACTT GGAATGGTTC CGCTTCAGTG GTCGAATTCT CGGCCTGGCT 
TTGATTCATC AGTATTTGTT GGACGCTTTC TTCACTCGGC CATTTTATAA AGCTCTTCTC 
AGACTACCCA CTGATCTCAG CGATCTGGAG TATCTGGATG AGGAGTTTCA CCAGAGCTTG 
CAGTGGATGA AGGAAAATGA CATCACTGAT GTGCTGGACC TCACCTTCAC TGTCAATGAG 
GAAGTGTTCG GACAGGTGAC AGAGCGGGAG CTGAAGTCTG GCGGGACGAA TGTGCAGGTG 
ACGGAGAAAA ATAAGAAGGA GTACATTGAG CGGATGGTGA AGTGGAGGGT GGAGCGAGGC 
GTTGTCCAGC AGACACAGGC TCTGGTGCGA GGATTTTATG AGGTGGTGGA CTCTCGTCTG 
GTGTCTGTGT TTGATGCCAG AGAGCTGGAG CTGGTCATCG CAGGAACAGC AGAGATCGAT 
TTGAACGACT GGAGAAACAA CACAGAGTAC AGAGGAGGTT ATCATGACGG GCACATTGTG 
ATCCGCTGGT TCTGGGGAGC AGTGGAGCGC TTCAATAATG AGCAGCGGCT GCGTCTGCTG 
CAGTTTGTGA CGGGCACCTC CAGCGTGCCG TATGAGGGCT TTACCGCCCT ACGTGGCAGC 
AATGGACTGA GACGCTTCTG CATTGAGAAA TGGGGAAAGA TCACATCACT CCCAAGGGCG 
CACACATGCT TTAACCGTCT GGATCTTCCA CCATATCCAT CCTACACGAT GTTATACGAG 
AAACTCCTGA TCGCTGTGGA AGAGACCAGC ACTTTTGGCC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hecw1b ( XM_021480876.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336551.1 Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), transcript variant X2, mRNA.

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CloneID	ODa150355
Clone ID Related Accession (Same CDS sequence)	XM_021480876.1
Accession Version	XM_021480876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGAGG AGGTGATGAT TCACCGCCAG GTGATACTGA CCCAGAACTT GTACCAGAGC 
AGGTTTCTCG GGCTTGCAGC TATGGCCTCT CCATCTCGTG CATCTCAGGG CAGGAGGCGA 
TGTAAGGACC CGATCCGTCA CAGTTTTAAC CCTGAGCAGT TCCCTAATGT GGACTTCCAG 
AACGGCGTGG CGGAGGATCT GGCGGCGGCC GTGCTGCGCT CTACCAGCGA CACTGACCTG 
CTGACATCCC ACTGCCGCTC CACCCTCACC GTTAGCACCT CCAGCTACAC CATCGGTCAG 
ACGCAGGACA TCACCCTCAG CTGGGACATC AAGGAGGAGG TGGACGCCGG AGACTGGATT 
GGCATGTACC TCATTGATGA GGTTCTGTCA GAGAATTTCC TGGACTACAA AAACCGGGGT 
GTGAACGGCT CGCACAAAGG GCAGATTATC TGGAAGATTG AGGCCAGCTC CTATTTTGTG 
GAACCGGAGA CTAAGATCTG TTTTAAATAT TATCATGGAG TTAGTGGAGC GCTTCGGGCC 
ACAACACCAT GCGTGACTGT GAAGAACCCC TCTGCACCGG TGTTTAAGCC GGTGGCCACA 
GACGATGTTC CTCAGAGTCA GGGAAGAAGG AGACTCATCA GCTTCTCTCT GTCAGATTTC 
CAGGCCACCG GACTGAAAAA AGGCATGTTT TTTAACCCCG ACCCCTACCT AAAGATCGCT 
ATCCACCCTG GCAAACACAG CATCTTCCCA GCGCTGCCTC ACCATGGCCA GGAGAAGAGA 
TCCGGCATCG TCTGCAATAC CATTAATCCT GTTTGGCAGA GGGAGCGCTT TAATTTTGTG 
TCTCTGCCTA CGGATGTGTT GGAGATTGAG GTGAAGGACA AGTTCGCCAA AAGCCGTCCC 
ATCATAAAGC GTTTCTTGGG CAAGCTGTCC ATGCCAGTGC AAAGACTACT GGAGAAGCAT 
GCTATCGGTG ATCGAGTGGT CAGCTATACT CTGGGTCGAA GACTTCCAAC AGAGCATGTC 
AGCGGGCAGC TGCAGTTCAG GTTCGAGATC ACCTCCTCCA TTCATCCAGA TGATGAAGAC 
ATGTCTCTCA GCACAGAGCA GCCGGTGGCA GCAGCAGAAG CTCAAGCTCA GCCTCAAGCT 
GAAGACACCA TGAGTTTAGA TCTCGGAGCC CCGGAGCTGC CCTTAGATGG AGATACGGCT 
TCAGTCGAGA TGCCCATGGA TGTTGAATCT GGTCAGAGTT GTGCAGCTGA AGCAGATGTC 
ATAGAGGAAG AGTGTTTAGA GGAAGAACAG CAGGAAGGAG AAGCAGCAGC AGCAGCAGCA 
GCAATGGAGC AAAGTGAAAC CAATGCAGAA GAAACAACAC AAGTCTTGGA TCCCATCAAT 
GGCGCTGAAG AAGAAGAAGC AGAGAATAAT TGCGCCTTTG CAGAAGAGTC TTCAGATGCT 
CGCGCAGGAG AGGAAGATGA AGCGATTTCA GATCCGCAGC CAGCTTCAGA AGAGCCGGAT 
GCGGATTTAG AGGAAGACTG TTCACTGAGG ATCCGAACCA CTCCAAGAAG GAAACCCCGT 
CCCTGCTCTT TACCTGTCTC TGAGCTTGAG ACGGTCATTG CTTCAGCCTG TGGGGAACCT 
GAAACGCCAC GCTCTCATTA CATCCGAATA CACCACCTGC TCCACAGCCT GCCGGCCACT 
CAACCGCAGA CAGGAGACTC CGCCGGGCCT GAACCAGACC GACCAATGGG TGTAGAGGAG 
GAAGATGAAG AGGAAGAAGA AGAAGGTGTA ACTCTTGAAG ATCATGCTTT AACACCCAAT 
GGCCCTCGCA GGACTCTTCC TCGCAGCCGC TCTCACGAAC GCCTCTCTGA CCTCATTCAG 
ATGCTGGATG GAGATGGACA TCCGCTTGGG GCTGAGGGGC AAACCGGAAA TCAGAGGTCA 
CCTGATTTGA GCCCAGTGGC GTGCTGCAGT CACATGTCCC CGAGAGCTTC TGGTCCCATT 
CGGGCACAAT CCCTAGACAG TGCGCGGAGG TCAGAGAGTA CGGTGTTCTC CTCGCAGGAT 
GACGAGGACG AGATGGAGCA GCCAAATGGC ATTCTGGGAT CAGAGACGGA AACAGAGCAG 
GGCGCTCAAT GTCGGGAGCC AGGCGACGGG AGCTGTCAGT GGGAGGAGGC ACCGGACAGT 
CACGTTCAGA GCCCTCATGG CATTGTGGGT AATGGAGAAG TGGCCGGACC GAGCGGCAGA 
CTGAGTCAGC TGCCTGCCCT GCGGCCCGAT CCACACCACT ATCCCACCAT TGATGAGCCG 
CTGCCGCCCA ACTGGGAGGC CCGGATCGAC AGTCACGGTC GTGTGTTTTA TGTGGATCAC 
ATTAACCGGA CCACCACTTG GCAAAGACCC ACATCTGCAG CCACGCCGGA CGGCCTACGC 
AGGTCTGGAT CCGTCCAACA GATGGAGCAG CTCAACAGGA GGTATCAGAC AATCCAGAGG 
ACGATGGCCA CCGAGAGGAG AGAAGAGGAA TCAGGAAGCC CCCGTAATGA CAGAACAGCC 
AATACTGAGA CAGACTCACC TCTGCAAACA AACGAGTTGA GGAGAGACAC CTCATGTTCT 
CCAGGCCACT GTCAGAAGAT TACGTTACTG TTACAGAGTC CAGCGGTCAA ATTCATCACT 
CACCCAGAGT TCTTCACTGT TCTCCACGCT AATTATGGTG CATATCGAAT GTTCACCAAC 
AGCTCGTGTC TGAAACACAT GGTCCTGAAA ATTCGACGAG ATGCTCGAAA TTTTGAGCGC 
TACCAGCACA ATCGTGACCT GGTGACGTTT CTGAACAGGT TTGCAGACGC TCAGCTGGAG 
CTGCCGCGCG GCTGGGAGAT CAAAACTGAC CCGCAGGGGA AGTCATTTTT TGTAGACCAC 
AACAGTCGTG CCACAACCTT CATCGATCCC AGAATCCCCT TGCAAAACGG CAGATTACCA 
AACCACCTGA CGCACCGCCA GCACCTGCAG AGATTACGCA GCTACAGCGC AGGAGAGGCA 
TCTGAGGTGT CGCGCACCCG AGGGGTGTCT TTGTTAGCCA GGCCTGGAAA CAGCCTGGTA 
GCAGCCATAC GAAACCAAAG CCAGCAGGAG CCTGTGCCAT TAGCGTACAA TGATAAAATT 
GTAGCATTTT TACGGCAACC AAACATCTTC GAGGTGCTAC AGGAGCGTCA GCCCAGCTTT 
GCCAGGAATC ACTCACTCAA GGATAAAGTG CATCACATCC GAACCGAAGG CACACAGAGA 
CTGGAGAAGC TGTCATGTGA TGCTGACCTG GTCATGCTGT TAAGTTTGTT TGAGGAGGAC 
ATCATGTCTT ACGTTCCTCT CGCATCATTT CACCCCGGGT TCAACTTTTC ACCTCGCTGC 
TCTCCTGGTT CTTCTCCCCA GAACTCTCCA GGCTTTCTGG AGCGAACTCC TCTCGTTCCA 
GCCTGCTGTC TGTCTGGTGA GAGCTCTCCA GGGACCCAGA GGGCTCGGGC TCCAGCGCCG 
TACAGAAGAG ACTTTGAGGC CAAATTGCGC AACTTCTACA GGAAGCTTGA GGCAAAGGGC 
TACGGGCAAG GGCCTGGGAA GATAAAATTG ATCGTCAGAA GAGATCATCT TTTAGAAGGA 
ACGTTTAACC AGGTCATGGC CTACTCACGT AAAGAGCTGC AGAGAAACAA GCTGTACATT 
ACCTTTGTGG GAGAGGAAGG TCTGGACTAC AGTGGCCCAT CGCGAGAGTT CTTCTTCCTG 
CTGTCTCAGG AGCTGTTTAA CCCTTACTAT GGCCTGTTTG AATACTCCGC CAACGACACA 
TACACAGTCC AGATCAGCCC CATGTCTGCT TTTGTGGAGA ACCACTTGGA ATGGTTCCGC 
TTCAGTGGTC GAATTCTCGG CCTGGCTTTG ATTCATCAGT ATTTGTTGGA CGCTTTCTTC 
ACTCGGCCAT TTTATAAAGC TCTTCTCAGA CTACCCACTG ATCTCAGCGA TCTGGAGTAT 
CTGGATGAGG AGTTTCACCA GAGCTTGCAG TGGATGAAGG AAAATGACAT CACTGATGTG 
CTGGACCTCA CCTTCACTGT CAATGAGGAA GTGTTCGGAC AGGTGACAGA GCGGGAGCTG 
AAGTCTGGCG GGACGAATGT GCAGGTGACG GAGAAAAATA AGAAGGAGTA CATTGAGCGG 
ATGGTGAAGT GGAGGGTGGA GCGAGGCGTT GTCCAGCAGA CACAGGCTCT GGTGCGAGGA 
TTTTATGAGG TGGTGGACTC TCGTCTGGTG TCTGTGTTTG ATGCCAGAGA GCTGGAGCTG 
GTCATCGCAG GAACAGCAGA GATCGATTTG AACGACTGGA GAAACAACAC AGAGTACAGA 
GGAGGTTATC ATGACGGGCA CATTGTGATC CGCTGGTTCT GGGGAGCAGT GGAGCGCTTC 
AATAATGAGC AGCGGCTGCG TCTGCTGCAG TTTGTGACGG GCACCTCCAG CGTGCCGTAT 
GAGGGCTTTA CCGCCCTACG TGGCAGCAAT GGACTGAGAC GCTTCTGCAT TGAGAAATGG 
GGAAAGATCA CATCACTCCC AAGGGCGCAC ACATGCTTTA ACCGTCTGGA TCTTCCACCA 
TATCCATCCT ACACGATGTT ATACGAGAAA CTCCTGATCG CTGTGGAAGA GACCAGCACT 
TTTGGCCTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336551.1
CDS	152..4786
Translation	MNEEVMIHRQVILTQNLYQSRFLGLAAMASPSRASQGRRRCKDPIRHSFNPEQFPNVDFQNGVAEDLAAAVLRSTSDTDLLTSHCRSTLTVSTSSYTIGQTQDITLSWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHKGQIIWKIEASSYFVEPETKICFKYYHGVSGALRATTPCVTVKNPSAPVFKPVATDDVPQSQGRRRLISFSLSDFQATGLKKGMFFNPDPYLKIAIHPGKHSIFPALPHHGQEKRSGIVCNTINPVWQRERFNFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLEKHAIGDRVVSYTLGRRLPTEHVSGQLQFRFEITSSIHPDDEDMSLSTEQPVAAAEAQAQPQAEDTMSLDLGAPELPLDGDTASVEMPMDVESGQSCAAEADVIEEECLEEEQQEGEAAAAAAAMEQSETNAEETTQVLDPINGAEEEEAENNCAFAEESSDARAGEEDEAISDPQPASEEPDADLEEDCSLRIRTTPRRKPRPCSLPVSELETVIASACGEPETPRSHYIRIHHLLHSLPATQPQTGDSAGPEPDRPMGVEEEDEEEEEEGVTLEDHALTPNGPRRTLPRSRSHERLSDLIQMLDGDGHPLGAEGQTGNQRSPDLSPVACCSHMSPRASGPIRAQSLDSARRSESTVFSSQDDEDEMEQPNGILGSETETEQGAQCREPGDGSCQWEEAPDSHVQSPHGIVGNGEVAGPSGRLSQLPALRPDPHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPTSAATPDGLRRSGSVQQMEQLNRRYQTIQRTMATERREEESGSPRNDRTANTETDSPLQTNELRRDTSCSPGHCQKITLLLQSPAVKFITHPEFFTVLHANYGAYRMFTNSSCLKHMVLKIRRDARNFERYQHNRDLVTFLNRFADAQLELPRGWEIKTDPQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRTRGVSLLARPGNSLVAAIRNQSQQEPVPLAYNDKIVAFLRQPNIFEVLQERQPSFARNHSLKDKVHHIRTEGTQRLEKLSCDADLVMLLSLFEEDIMSYVPLASFHPGFNFSPRCSPGSSPQNSPGFLERTPLVPACCLSGESSPGTQRARAPAPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIVRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPTDLSDLEYLDEEFHQSLQWMKENDITDVLDLTFTVNEEVFGQVTERELKSGGTNVQVTEKNKKEYIERMVKWRVERGVVQQTQALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWGAVERFNNEQRLRLLQFVTGTSSVPYEGFTALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYTMLYEKLLIAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_021480876.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480876.1

ORF Insert Sequence:

ATGAACGAGG AGGTGATGAT TCACCGCCAG GTGATACTGA CCCAGAACTT GTACCAGAGC 
AGGTTTCTCG GGCTTGCAGC TATGGCCTCT CCATCTCGTG CATCTCAGGG CAGGAGGCGA 
TGTAAGGACC CGATCCGTCA CAGTTTTAAC CCTGAGCAGT TCCCTAATGT GGACTTCCAG 
AACGGCGTGG CGGAGGATCT GGCGGCGGCC GTGCTGCGCT CTACCAGCGA CACTGACCTG 
CTGACATCCC ACTGCCGCTC CACCCTCACC GTTAGCACCT CCAGCTACAC CATCGGTCAG 
ACGCAGGACA TCACCCTCAG CTGGGACATC AAGGAGGAGG TGGACGCCGG AGACTGGATT 
GGCATGTACC TCATTGATGA GGTTCTGTCA GAGAATTTCC TGGACTACAA AAACCGGGGT 
GTGAACGGCT CGCACAAAGG GCAGATTATC TGGAAGATTG AGGCCAGCTC CTATTTTGTG 
GAACCGGAGA CTAAGATCTG TTTTAAATAT TATCATGGAG TTAGTGGAGC GCTTCGGGCC 
ACAACACCAT GCGTGACTGT GAAGAACCCC TCTGCACCGG TGTTTAAGCC GGTGGCCACA 
GACGATGTTC CTCAGAGTCA GGGAAGAAGG AGACTCATCA GCTTCTCTCT GTCAGATTTC 
CAGGCCACCG GACTGAAAAA AGGCATGTTT TTTAACCCCG ACCCCTACCT AAAGATCGCT 
ATCCACCCTG GCAAACACAG CATCTTCCCA GCGCTGCCTC ACCATGGCCA GGAGAAGAGA 
TCCGGCATCG TCTGCAATAC CATTAATCCT GTTTGGCAGA GGGAGCGCTT TAATTTTGTG 
TCTCTGCCTA CGGATGTGTT GGAGATTGAG GTGAAGGACA AGTTCGCCAA AAGCCGTCCC 
ATCATAAAGC GTTTCTTGGG CAAGCTGTCC ATGCCAGTGC AAAGACTACT GGAGAAGCAT 
GCTATCGGTG ATCGAGTGGT CAGCTATACT CTGGGTCGAA GACTTCCAAC AGAGCATGTC 
AGCGGGCAGC TGCAGTTCAG GTTCGAGATC ACCTCCTCCA TTCATCCAGA TGATGAAGAC 
ATGTCTCTCA GCACAGAGCA GCCGGTGGCA GCAGCAGAAG CTCAAGCTCA GCCTCAAGCT 
GAAGACACCA TGAGTTTAGA TCTCGGAGCC CCGGAGCTGC CCTTAGATGG AGATACGGCT 
TCAGTCGAGA TGCCCATGGA TGTTGAATCT GGTCAGAGTT GTGCAGCTGA AGCAGATGTC 
ATAGAGGAAG AGTGTTTAGA GGAAGAACAG CAGGAAGGAG AAGCAGCAGC AGCAGCAGCA 
GCAATGGAGC AAAGTGAAAC CAATGCAGAA GAAACAACAC AAGTCTTGGA TCCCATCAAT 
GGCGCTGAAG AAGAAGAAGC AGAGAATAAT TGCGCCTTTG CAGAAGAGTC TTCAGATGCT 
CGCGCAGGAG AGGAAGATGA AGCGATTTCA GATCCGCAGC CAGCTTCAGA AGAGCCGGAT 
GCGGATTTAG AGGAAGACTG TTCACTGAGG ATCCGAACCA CTCCAAGAAG GAAACCCCGT 
CCCTGCTCTT TACCTGTCTC TGAGCTTGAG ACGGTCATTG CTTCAGCCTG TGGGGAACCT 
GAAACGCCAC GCTCTCATTA CATCCGAATA CACCACCTGC TCCACAGCCT GCCGGCCACT 
CAACCGCAGA CAGGAGACTC CGCCGGGCCT GAACCAGACC GACCAATGGG TGTAGAGGAG 
GAAGATGAAG AGGAAGAAGA AGAAGGTGTA ACTCTTGAAG ATCATGCTTT AACACCCAAT 
GGCCCTCGCA GGACTCTTCC TCGCAGCCGC TCTCACGAAC GCCTCTCTGA CCTCATTCAG 
ATGCTGGATG GAGATGGACA TCCGCTTGGG GCTGAGGGGC AAACCGGAAA TCAGAGGTCA 
CCTGATTTGA GCCCAGTGGC GTGCTGCAGT CACATGTCCC CGAGAGCTTC TGGTCCCATT 
CGGGCACAAT CCCTAGACAG TGCGCGGAGG TCAGAGAGTA CGGTGTTCTC CTCGCAGGAT 
GACGAGGACG AGATGGAGCA GCCAAATGGC ATTCTGGGAT CAGAGACGGA AACAGAGCAG 
GGCGCTCAAT GTCGGGAGCC AGGCGACGGG AGCTGTCAGT GGGAGGAGGC ACCGGACAGT 
CACGTTCAGA GCCCTCATGG CATTGTGGGT AATGGAGAAG TGGCCGGACC GAGCGGCAGA 
CTGAGTCAGC TGCCTGCCCT GCGGCCCGAT CCACACCACT ATCCCACCAT TGATGAGCCG 
CTGCCGCCCA ACTGGGAGGC CCGGATCGAC AGTCACGGTC GTGTGTTTTA TGTGGATCAC 
ATTAACCGGA CCACCACTTG GCAAAGACCC ACATCTGCAG CCACGCCGGA CGGCCTACGC 
AGGTCTGGAT CCGTCCAACA GATGGAGCAG CTCAACAGGA GGTATCAGAC AATCCAGAGG 
ACGATGGCCA CCGAGAGGAG AGAAGAGGAA TCAGGAAGCC CCCGTAATGA CAGAACAGCC 
AATACTGAGA CAGACTCACC TCTGCAAACA AACGAGTTGA GGAGAGACAC CTCATGTTCT 
CCAGGCCACT GTCAGAAGAT TACGTTACTG TTACAGAGTC CAGCGGTCAA ATTCATCACT 
CACCCAGAGT TCTTCACTGT TCTCCACGCT AATTATGGTG CATATCGAAT GTTCACCAAC 
AGCTCGTGTC TGAAACACAT GGTCCTGAAA ATTCGACGAG ATGCTCGAAA TTTTGAGCGC 
TACCAGCACA ATCGTGACCT GGTGACGTTT CTGAACAGGT TTGCAGACGC TCAGCTGGAG 
CTGCCGCGCG GCTGGGAGAT CAAAACTGAC CCGCAGGGGA AGTCATTTTT TGTAGACCAC 
AACAGTCGTG CCACAACCTT CATCGATCCC AGAATCCCCT TGCAAAACGG CAGATTACCA 
AACCACCTGA CGCACCGCCA GCACCTGCAG AGATTACGCA GCTACAGCGC AGGAGAGGCA 
TCTGAGGTGT CGCGCACCCG AGGGGTGTCT TTGTTAGCCA GGCCTGGAAA CAGCCTGGTA 
GCAGCCATAC GAAACCAAAG CCAGCAGGAG CCTGTGCCAT TAGCGTACAA TGATAAAATT 
GTAGCATTTT TACGGCAACC AAACATCTTC GAGGTGCTAC AGGAGCGTCA GCCCAGCTTT 
GCCAGGAATC ACTCACTCAA GGATAAAGTG CATCACATCC GAACCGAAGG CACACAGAGA 
CTGGAGAAGC TGTCATGTGA TGCTGACCTG GTCATGCTGT TAAGTTTGTT TGAGGAGGAC 
ATCATGTCTT ACGTTCCTCT CGCATCATTT CACCCCGGGT TCAACTTTTC ACCTCGCTGC 
TCTCCTGGTT CTTCTCCCCA GAACTCTCCA GGCTTTCTGG AGCGAACTCC TCTCGTTCCA 
GCCTGCTGTC TGTCTGGTGA GAGCTCTCCA GGGACCCAGA GGGCTCGGGC TCCAGCGCCG 
TACAGAAGAG ACTTTGAGGC CAAATTGCGC AACTTCTACA GGAAGCTTGA GGCAAAGGGC 
TACGGGCAAG GGCCTGGGAA GATAAAATTG ATCGTCAGAA GAGATCATCT TTTAGAAGGA 
ACGTTTAACC AGGTCATGGC CTACTCACGT AAAGAGCTGC AGAGAAACAA GCTGTACATT 
ACCTTTGTGG GAGAGGAAGG TCTGGACTAC AGTGGCCCAT CGCGAGAGTT CTTCTTCCTG 
CTGTCTCAGG AGCTGTTTAA CCCTTACTAT GGCCTGTTTG AATACTCCGC CAACGACACA 
TACACAGTCC AGATCAGCCC CATGTCTGCT TTTGTGGAGA ACCACTTGGA ATGGTTCCGC 
TTCAGTGGTC GAATTCTCGG CCTGGCTTTG ATTCATCAGT ATTTGTTGGA CGCTTTCTTC 
ACTCGGCCAT TTTATAAAGC TCTTCTCAGA CTACCCACTG ATCTCAGCGA TCTGGAGTAT 
CTGGATGAGG AGTTTCACCA GAGCTTGCAG TGGATGAAGG AAAATGACAT CACTGATGTG 
CTGGACCTCA CCTTCACTGT CAATGAGGAA GTGTTCGGAC AGGTGACAGA GCGGGAGCTG 
AAGTCTGGCG GGACGAATGT GCAGGTGACG GAGAAAAATA AGAAGGAGTA CATTGAGCGG 
ATGGTGAAGT GGAGGGTGGA GCGAGGCGTT GTCCAGCAGA CACAGGCTCT GGTGCGAGGA 
TTTTATGAGG TGGTGGACTC TCGTCTGGTG TCTGTGTTTG ATGCCAGAGA GCTGGAGCTG 
GTCATCGCAG GAACAGCAGA GATCGATTTG AACGACTGGA GAAACAACAC AGAGTACAGA 
GGAGGTTATC ATGACGGGCA CATTGTGATC CGCTGGTTCT GGGGAGCAGT GGAGCGCTTC 
AATAATGAGC AGCGGCTGCG TCTGCTGCAG TTTGTGACGG GCACCTCCAG CGTGCCGTAT 
GAGGGCTTTA CCGCCCTACG TGGCAGCAAT GGACTGAGAC GCTTCTGCAT TGAGAAATGG 
GGAAAGATCA CATCACTCCC AAGGGCGCAC ACATGCTTTA ACCGTCTGGA TCTTCCACCA 
TATCCATCCT ACACGATGTT ATACGAGAAA CTCCTGATCG CTGTGGAAGA GACCAGCACT 
TTTGGCCTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hecw1b ( NM_001145764.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001139236.1 Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), mRNA.

Price & Availability↑

CloneID	ODa35770
Clone ID Related Accession (Same CDS sequence)	NM_001145764.1
Accession Version	NM_001145764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase HECW1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX005176.8 and BX324139.11. On Mar 12, 2009 this sequence version replaced XM_687088.3. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAACGAGG AGGTGATGAT TCACCGCCAG GTGATACTGA CCCAGAACTT GTACCAGAGC 
AGGTTTCTCG GGCTTGCAGC TATGGCCTCT CCATCTCGTG CATCTCAGGG CAGGAGGCGA 
TGTAAGGACC CGATCCGTCA CAGTTTTAAC CCTGAGCAGT TCCCTAATGT GGACTTCCAG 
AACGGCGTGG CGGAGGATCT GGCGGCGGCC GTGCTGCGCT CTACCAGCGA CACTGACCTG 
CTGACATCCC ACTGCCGCTC CACCCTCACC GTTAGCACCT CCAGCTACAC CATCGGTCAG 
ACGCAGGACA TCACCCTCAG CTGGGACATC AAGGAGGAGG TGGACGCCGG AGACTGGATT 
GGCATGTACC TCATTGATGA GGTTCTGTCA GAGAATTTCC TGGACTACAA AAACCGGGGT 
GTGAACGGCT CGCACAAAGG GCAGATTATC TGGAAGATTG AGGCCAGCTC CTATTTTGTG 
GAACCGGAGA CTAAGATCTG TTTTAAATAT TATCATGGAG TTAGTGGAGC GCTTCGGGCC 
ACAACACCAT GCGTGACTGT GAAGAACCCC TCTGCACCGG TGTTTAAGCC GGTGGCCACA 
GACGATGTTC CTCAGAGTCA GGGAAGAAGG AGACTCATCA GCTTCTCTCT GTCAGATTTC 
CAGGCCACCG GACTGAAAAA AGGCATGTTT TTTAACCCCG ACCCCTACCT AAAGATCGCT 
ATCCACCCTG GCAAACACAG CATCTTCCCA GCGCTGCCTC ACCATGGCCA GGAGAAGAGA 
TCCGGCATCG TCTGCAATAC CATTAATCCT GTTTGGCAGA GGGAGCGCTT TAATTTTGTG 
TCTCTGCCTA CGGATGTGTT GGAGATTGAG GTGAAGGACA AGTTCGCCAA AAGCCGTCCC 
ATCATAAAGC GTTTCTTGGG CAAGCTGTCC ATGCCAGTGC AAAGACTACT GGAGAAGCAT 
GCTATCGGTG ATCGAGTGGT CAGCTATACT CTGGGTCGAA GACTTCCAAC AGAGCATGTC 
AGCGGGCAGC TGCAGTTCAG GTTCGAGATC ACCTCCTCCA TTCATCCAGA TGATGAAGAC 
ATGTCTCTCA GCACAGAGCA GCCGGTGGCA GCAGCAGAAG CTCAAGCTCA GCCTCAAGCT 
GAAGACACCA TGAGTTTAGA TCTCGGAGCC CCGGAGCTGC CCTTAGATGG AGATACGGCT 
TCAGTCGAGA TGCCCATGGA TGTTGAATCT GGTCAGAGTT GTGCAGCTGA AGCAGATGTC 
ATAGAGGAAG AGTGTTTAGA GGAAGAACAG CAGGAAGGAG AAGCAGCAGC AGCAGCAGCA 
GCAATGGAGC AAAGTGAAAC CAATGCAGAA GAAACAACAC AAGTCTTGGA TCCCATCAAT 
GGCGCTGAAG AAGAAGAAGC AGAGAATAAT TGCGCCTTTG CAGAAGAGTC TTCAGATGCT 
CGCGCAGGAG AGGAAGATGA AGCGATTTCA GATCCGCAGC CAGCTTCAGA AGAGCCGGAT 
GCGGATTTAG AGGAAGACTG TTCACTGAGG ATCCGAACCA CTCCAAGAAG GAAACCCCGT 
CCCTGCTCTT TACCTGTCTC TGAGCTTGAG ACGGTCATTG CTTCAGCCTG TGGGGAACCT 
GAAACGCCAC GCTCTCATTA CATCCGAATA CACCACCTGC TCCACAGCCT GCCGGCCACT 
CAACCGCAGA CAGGAGACTC CGCCGGGCCT GAACCAGACC GACCAATGGG TGTAGAGGAG 
GAAGATGAAG AGGAAGAAGA AGAAGGTGTA ACTCTTGAAG ATCATGCTTT AACACCCAAT 
GGCCCTCGCA GGACTCTTCC TCGCAGCCGC TCTCACGAAC GCCTCTCTGA CCTCATTCAG 
ATGCTGGATG GAGATGGACA TCCGCTTGGG GCTGAGGGGC AAACCGGAAA TCAGAGGTCA 
CCTGATTTGA GCCCAGTGGC GTGCTGCAGT CACATGTCCC CGAGAGCTTC TGGTCCCATT 
CGGGCACAAT CCCTAGACAG TGCGCGGAGG TCAGAGAGTA CGGTGTTCTC CTCGCAGGAT 
GACGAGGACG AGATGGAGCA GCCAAATGGC ATTCTGGGAT CAGAGACGGA AACAGAGCAG 
GGCGCTCAAT GTCGGGAGCC AGGCGACGGG AGCTGTCAGT GGGAGGAGGC ACCGGACAGT 
CACGTTCAGA GCCCTCATGG CATTGTGGGT AATGGAGAAG TGGCCGGACC GAGCGGCAGA 
CGAGAATGTC CTTTGCCTTG TAACCACCCT GCAGTGAGTC AGCTGCCTGC CCTGCGGCCC 
GATCCACACC ACTATCCCAC CATTGATGAG CCGCTGCCGC CCAACTGGGA GGCCCGGATC 
GACAGTCACG GTCGTGTGTT TTATGTGGAT CACATTAACC GGACCACCAC TTGGCAAAGA 
CCCACATCTG CAGCCACGCC GGACGGCCTA CGCAGGTCTG GATCCGTCCA ACAGATGGAG 
CAGCTCAACA GGAGGTATCA GACAATCCAG AGGACGATGG CCACCGAGAG GAGAGAAGAG 
GAATCAGGAA GCCCCCGTAA TGACAGAACA GCCAATACTG AGACAGACTC ACCTCTGCAA 
ACAAACGGTG ACTTCACACA CATCACACAT AATGATCGTT CGTCCTGTTC AGTTAAAAGG 
TTGAGGAGAG ACACCTCATG TTCTCCAGGC CACTGTCAGA AGATTACGTT ACTGTTACAG 
AGTCCAGCGG TCAAATTCAT CACTCACCCA GAGTTCTTCA CTGTTCTCCA CGCTAATTAT 
GGTGCATATC GAATGTTCAC CAACAGCTCG TGTCTGAAAC ACATGGTCCT GAAAATTCGA 
CGAGATGCTC GAAATTTTGA GCGCTACCAG CACAATCGTG ACCTGGTGAC GTTTCTGAAC 
AGGTTTGCAG ACGCTCAGCT GGAGCTGCCG CGCGGCTGGG AGATCAAAAC TGACCCGCAG 
GGGAAGTCAT TTTTTGTAGA CCACAACAGT CGTGCCACAA CCTTCATCGA TCCCAGAATC 
CCCTTGCAAA ACGGCAGATT ACCAAACCAC CTGACGCACC GCCAGCACCT GCAGAGATTA 
CGCAGCTACA GCGCAGGAGA GGCATCTGAG GTGTCGCGCA CCCGAGGGGT GTCTTTGTTA 
GCCAGGCCTG GAAACAGCCT GGTAGCAGCC ATACGAAACC AAAGCCAGCA GGAGCCTGTG 
CCATTAGCGT ACAATGATAA AATTGTAGCA TTTTTACGGC AACCAAACAT CTTCGAGGTG 
CTACAGGAGC GTCAGCCCAG CTTTGCCAGG AATCACTCAC TCAAGGATAA AGTGCATCAC 
ATCCGAACCG AAGGCACACA GAGACTGGAG AAGCTGTCAT GTGATGCTGA CCTGGTCATG 
CTGTTAAGTT TGTTTGAGGA GGACATCATG TCTTACGTTC CTCTCGCATC ATTTCACCCC 
GGGTTCAACT TTTCACCTCG CTGCTCTCCT GGTTCTTCTC CCCAGAACTC TCCAGGGACC 
CAGAGGGCTC GGGCTCCAGC GCCGTACAGA AGAGACTTTG AGGCCAAATT GCGCAACTTC 
TACAGGAAGC TTGAGGCAAA GGGCTACGGG CAAGGGCCTG GGAAGATAAA ATTGATCGTC 
AGAAGAGATC ATCTTTTAGA AGGAACGTTT AACCAGGTCA TGGCCTACTC ACGTAAAGAG 
CTGCAGAGAA ACAAGCTGTA CATTACCTTT GTGGGAGAGG AAGGTCTGGA CTACAGTGGC 
CCATCGCGAG AGTTCTTCTT CCTGCTGTCT CAGGAGCTGT TTAACCCTTA CTATGGCCTG 
TTTGAATACT CCGCCAACGA CACATACACA GTCCAGATCA GCCCCATGTC TGCTTTTGTG 
GAGAACCACT TGGAATGGTT CCGCTTCAGT GGTCGAATTC TCGGCCTGGC TTTGATTCAT 
CAGTATTTGT TGGACGCTTT CTTCACTCGG CCATTTTATA AAGCTCTTCT CAGACTACCC 
ACTGATCTCA GCGATCTGGA GTATCTGGAT GAGGAGTTTC ACCAGAGCTT GCAGTGGATG 
AAGGAAAATG ACATCACTGA TGTGCTGGAC CTCACCTTCA CTGTCAATGA GGAAGTGTTC 
GGACAGGTGA CAGAGCGGGA GCTGAAGTCT GGCGGGACGA ATGTGCAGGT GACGGAGAAA 
AATAAGAAGG AGTACATTGA GCGGATGGTG AAGTGGAGGG TGGAGCGAGG CGTTGTCCAG 
CAGACACAGG CTCTGGTGCG AGGATTTTAT GAGGTGGTGG ACTCTCGTCT GGTGTCTGTG 
TTTGATGCCA GAGAGCTGGA GCTGGTCATC GCAGGAACAG CAGAGATCGA TTTGAACGAC 
TGGAGAAACA ACACAGAGTA CAGAGGAGGT TATCATGACG GGCACATTGT GATCCGCTGG 
TTCTGGGGAG CAGTGGAGCG CTTCAATAAT GAGCAGCGGC TGCGTCTGCT GCAGTTTGTG 
ACGGGCACCT CCAGCGTGCC GTATGAGGGC TTTACCGCCC TACGTGGCAG CAATGGACTG 
AGACGCTTCT GCATTGAGAA ATGGGGAAAG ATCACATCAC TCCCAAGGGC GCACACATGC 
TTTAACCGTC TGGATCTTCC ACCATATCCA TCCTACACGA TGTTATACGA GAAACTCCTG 
ATCGCTGTGG AAGAGACCAG CACTTTTGGC CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139236.1
CDS	1..4659
Translation	MNEEVMIHRQVILTQNLYQSRFLGLAAMASPSRASQGRRRCKDPIRHSFNPEQFPNVDFQNGVAEDLAAAVLRSTSDTDLLTSHCRSTLTVSTSSYTIGQTQDITLSWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHKGQIIWKIEASSYFVEPETKICFKYYHGVSGALRATTPCVTVKNPSAPVFKPVATDDVPQSQGRRRLISFSLSDFQATGLKKGMFFNPDPYLKIAIHPGKHSIFPALPHHGQEKRSGIVCNTINPVWQRERFNFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLEKHAIGDRVVSYTLGRRLPTEHVSGQLQFRFEITSSIHPDDEDMSLSTEQPVAAAEAQAQPQAEDTMSLDLGAPELPLDGDTASVEMPMDVESGQSCAAEADVIEEECLEEEQQEGEAAAAAAAMEQSETNAEETTQVLDPINGAEEEEAENNCAFAEESSDARAGEEDEAISDPQPASEEPDADLEEDCSLRIRTTPRRKPRPCSLPVSELETVIASACGEPETPRSHYIRIHHLLHSLPATQPQTGDSAGPEPDRPMGVEEEDEEEEEEGVTLEDHALTPNGPRRTLPRSRSHERLSDLIQMLDGDGHPLGAEGQTGNQRSPDLSPVACCSHMSPRASGPIRAQSLDSARRSESTVFSSQDDEDEMEQPNGILGSETETEQGAQCREPGDGSCQWEEAPDSHVQSPHGIVGNGEVAGPSGRRECPLPCNHPAVSQLPALRPDPHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPTSAATPDGLRRSGSVQQMEQLNRRYQTIQRTMATERREEESGSPRNDRTANTETDSPLQTNGDFTHITHNDRSSCSVKRLRRDTSCSPGHCQKITLLLQSPAVKFITHPEFFTVLHANYGAYRMFTNSSCLKHMVLKIRRDARNFERYQHNRDLVTFLNRFADAQLELPRGWEIKTDPQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRTRGVSLLARPGNSLVAAIRNQSQQEPVPLAYNDKIVAFLRQPNIFEVLQERQPSFARNHSLKDKVHHIRTEGTQRLEKLSCDADLVMLLSLFEEDIMSYVPLASFHPGFNFSPRCSPGSSPQNSPGTQRARAPAPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIVRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPTDLSDLEYLDEEFHQSLQWMKENDITDVLDLTFTVNEEVFGQVTERELKSGGTNVQVTEKNKKEYIERMVKWRVERGVVQQTQALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWGAVERFNNEQRLRLLQFVTGTSSVPYEGFTALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYTMLYEKLLIAVEETSTFGLE

Target ORF information:

RefSeq Version	NM_001145764.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145764.1

ORF Insert Sequence:

ATGAACGAGG AGGTGATGAT TCACCGCCAG GTGATACTGA CCCAGAACTT GTACCAGAGC 
AGGTTTCTCG GGCTTGCAGC TATGGCCTCT CCATCTCGTG CATCTCAGGG CAGGAGGCGA 
TGTAAGGACC CGATCCGTCA CAGTTTTAAC CCTGAGCAGT TCCCTAATGT GGACTTCCAG 
AACGGCGTGG CGGAGGATCT GGCGGCGGCC GTGCTGCGCT CTACCAGCGA CACTGACCTG 
CTGACATCCC ACTGCCGCTC CACCCTCACC GTTAGCACCT CCAGCTACAC CATCGGTCAG 
ACGCAGGACA TCACCCTCAG CTGGGACATC AAGGAGGAGG TGGACGCCGG AGACTGGATT 
GGCATGTACC TCATTGATGA GGTTCTGTCA GAGAATTTCC TGGACTACAA AAACCGGGGT 
GTGAACGGCT CGCACAAAGG GCAGATTATC TGGAAGATTG AGGCCAGCTC CTATTTTGTG 
GAACCGGAGA CTAAGATCTG TTTTAAATAT TATCATGGAG TTAGTGGAGC GCTTCGGGCC 
ACAACACCAT GCGTGACTGT GAAGAACCCC TCTGCACCGG TGTTTAAGCC GGTGGCCACA 
GACGATGTTC CTCAGAGTCA GGGAAGAAGG AGACTCATCA GCTTCTCTCT GTCAGATTTC 
CAGGCCACCG GACTGAAAAA AGGCATGTTT TTTAACCCCG ACCCCTACCT AAAGATCGCT 
ATCCACCCTG GCAAACACAG CATCTTCCCA GCGCTGCCTC ACCATGGCCA GGAGAAGAGA 
TCCGGCATCG TCTGCAATAC CATTAATCCT GTTTGGCAGA GGGAGCGCTT TAATTTTGTG 
TCTCTGCCTA CGGATGTGTT GGAGATTGAG GTGAAGGACA AGTTCGCCAA AAGCCGTCCC 
ATCATAAAGC GTTTCTTGGG CAAGCTGTCC ATGCCAGTGC AAAGACTACT GGAGAAGCAT 
GCTATCGGTG ATCGAGTGGT CAGCTATACT CTGGGTCGAA GACTTCCAAC AGAGCATGTC 
AGCGGGCAGC TGCAGTTCAG GTTCGAGATC ACCTCCTCCA TTCATCCAGA TGATGAAGAC 
ATGTCTCTCA GCACAGAGCA GCCGGTGGCA GCAGCAGAAG CTCAAGCTCA GCCTCAAGCT 
GAAGACACCA TGAGTTTAGA TCTCGGAGCC CCGGAGCTGC CCTTAGATGG AGATACGGCT 
TCAGTCGAGA TGCCCATGGA TGTTGAATCT GGTCAGAGTT GTGCAGCTGA AGCAGATGTC 
ATAGAGGAAG AGTGTTTAGA GGAAGAACAG CAGGAAGGAG AAGCAGCAGC AGCAGCAGCA 
GCAATGGAGC AAAGTGAAAC CAATGCAGAA GAAACAACAC AAGTCTTGGA TCCCATCAAT 
GGCGCTGAAG AAGAAGAAGC AGAGAATAAT TGCGCCTTTG CAGAAGAGTC TTCAGATGCT 
CGCGCAGGAG AGGAAGATGA AGCGATTTCA GATCCGCAGC CAGCTTCAGA AGAGCCGGAT 
GCGGATTTAG AGGAAGACTG TTCACTGAGG ATCCGAACCA CTCCAAGAAG GAAACCCCGT 
CCCTGCTCTT TACCTGTCTC TGAGCTTGAG ACGGTCATTG CTTCAGCCTG TGGGGAACCT 
GAAACGCCAC GCTCTCATTA CATCCGAATA CACCACCTGC TCCACAGCCT GCCGGCCACT 
CAACCGCAGA CAGGAGACTC CGCCGGGCCT GAACCAGACC GACCAATGGG TGTAGAGGAG 
GAAGATGAAG AGGAAGAAGA AGAAGGTGTA ACTCTTGAAG ATCATGCTTT AACACCCAAT 
GGCCCTCGCA GGACTCTTCC TCGCAGCCGC TCTCACGAAC GCCTCTCTGA CCTCATTCAG 
ATGCTGGATG GAGATGGACA TCCGCTTGGG GCTGAGGGGC AAACCGGAAA TCAGAGGTCA 
CCTGATTTGA GCCCAGTGGC GTGCTGCAGT CACATGTCCC CGAGAGCTTC TGGTCCCATT 
CGGGCACAAT CCCTAGACAG TGCGCGGAGG TCAGAGAGTA CGGTGTTCTC CTCGCAGGAT 
GACGAGGACG AGATGGAGCA GCCAAATGGC ATTCTGGGAT CAGAGACGGA AACAGAGCAG 
GGCGCTCAAT GTCGGGAGCC AGGCGACGGG AGCTGTCAGT GGGAGGAGGC ACCGGACAGT 
CACGTTCAGA GCCCTCATGG CATTGTGGGT AATGGAGAAG TGGCCGGACC GAGCGGCAGA 
CGAGAATGTC CTTTGCCTTG TAACCACCCT GCAGTGAGTC AGCTGCCTGC CCTGCGGCCC 
GATCCACACC ACTATCCCAC CATTGATGAG CCGCTGCCGC CCAACTGGGA GGCCCGGATC 
GACAGTCACG GTCGTGTGTT TTATGTGGAT CACATTAACC GGACCACCAC TTGGCAAAGA 
CCCACATCTG CAGCCACGCC GGACGGCCTA CGCAGGTCTG GATCCGTCCA ACAGATGGAG 
CAGCTCAACA GGAGGTATCA GACAATCCAG AGGACGATGG CCACCGAGAG GAGAGAAGAG 
GAATCAGGAA GCCCCCGTAA TGACAGAACA GCCAATACTG AGACAGACTC ACCTCTGCAA 
ACAAACGGTG ACTTCACACA CATCACACAT AATGATCGTT CGTCCTGTTC AGTTAAAAGG 
TTGAGGAGAG ACACCTCATG TTCTCCAGGC CACTGTCAGA AGATTACGTT ACTGTTACAG 
AGTCCAGCGG TCAAATTCAT CACTCACCCA GAGTTCTTCA CTGTTCTCCA CGCTAATTAT 
GGTGCATATC GAATGTTCAC CAACAGCTCG TGTCTGAAAC ACATGGTCCT GAAAATTCGA 
CGAGATGCTC GAAATTTTGA GCGCTACCAG CACAATCGTG ACCTGGTGAC GTTTCTGAAC 
AGGTTTGCAG ACGCTCAGCT GGAGCTGCCG CGCGGCTGGG AGATCAAAAC TGACCCGCAG 
GGGAAGTCAT TTTTTGTAGA CCACAACAGT CGTGCCACAA CCTTCATCGA TCCCAGAATC 
CCCTTGCAAA ACGGCAGATT ACCAAACCAC CTGACGCACC GCCAGCACCT GCAGAGATTA 
CGCAGCTACA GCGCAGGAGA GGCATCTGAG GTGTCGCGCA CCCGAGGGGT GTCTTTGTTA 
GCCAGGCCTG GAAACAGCCT GGTAGCAGCC ATACGAAACC AAAGCCAGCA GGAGCCTGTG 
CCATTAGCGT ACAATGATAA AATTGTAGCA TTTTTACGGC AACCAAACAT CTTCGAGGTG 
CTACAGGAGC GTCAGCCCAG CTTTGCCAGG AATCACTCAC TCAAGGATAA AGTGCATCAC 
ATCCGAACCG AAGGCACACA GAGACTGGAG AAGCTGTCAT GTGATGCTGA CCTGGTCATG 
CTGTTAAGTT TGTTTGAGGA GGACATCATG TCTTACGTTC CTCTCGCATC ATTTCACCCC 
GGGTTCAACT TTTCACCTCG CTGCTCTCCT GGTTCTTCTC CCCAGAACTC TCCAGGGACC 
CAGAGGGCTC GGGCTCCAGC GCCGTACAGA AGAGACTTTG AGGCCAAATT GCGCAACTTC 
TACAGGAAGC TTGAGGCAAA GGGCTACGGG CAAGGGCCTG GGAAGATAAA ATTGATCGTC 
AGAAGAGATC ATCTTTTAGA AGGAACGTTT AACCAGGTCA TGGCCTACTC ACGTAAAGAG 
CTGCAGAGAA ACAAGCTGTA CATTACCTTT GTGGGAGAGG AAGGTCTGGA CTACAGTGGC 
CCATCGCGAG AGTTCTTCTT CCTGCTGTCT CAGGAGCTGT TTAACCCTTA CTATGGCCTG 
TTTGAATACT CCGCCAACGA CACATACACA GTCCAGATCA GCCCCATGTC TGCTTTTGTG 
GAGAACCACT TGGAATGGTT CCGCTTCAGT GGTCGAATTC TCGGCCTGGC TTTGATTCAT 
CAGTATTTGT TGGACGCTTT CTTCACTCGG CCATTTTATA AAGCTCTTCT CAGACTACCC 
ACTGATCTCA GCGATCTGGA GTATCTGGAT GAGGAGTTTC ACCAGAGCTT GCAGTGGATG 
AAGGAAAATG ACATCACTGA TGTGCTGGAC CTCACCTTCA CTGTCAATGA GGAAGTGTTC 
GGACAGGTGA CAGAGCGGGA GCTGAAGTCT GGCGGGACGA ATGTGCAGGT GACGGAGAAA 
AATAAGAAGG AGTACATTGA GCGGATGGTG AAGTGGAGGG TGGAGCGAGG CGTTGTCCAG 
CAGACACAGG CTCTGGTGCG AGGATTTTAT GAGGTGGTGG ACTCTCGTCT GGTGTCTGTG 
TTTGATGCCA GAGAGCTGGA GCTGGTCATC GCAGGAACAG CAGAGATCGA TTTGAACGAC 
TGGAGAAACA ACACAGAGTA CAGAGGAGGT TATCATGACG GGCACATTGT GATCCGCTGG 
TTCTGGGGAG CAGTGGAGCG CTTCAATAAT GAGCAGCGGC TGCGTCTGCT GCAGTTTGTG 
ACGGGCACCT CCAGCGTGCC GTATGAGGGC TTTACCGCCC TACGTGGCAG CAATGGACTG 
AGACGCTTCT GCATTGAGAA ATGGGGAAAG ATCACATCAC TCCCAAGGGC GCACACATGC 
TTTAACCGTC TGGATCTTCC ACCATATCCA TCCTACACGA TGTTATACGA GAAACTCCTG 
ATCGCTGTGG AAGAGACCAG CACTTTTGGC CTTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hecw1b ( XM_005168424.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168481.1 Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), transcript variant X2, mRNA.

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CloneID	ODa07503
Clone ID Related Accession (Same CDS sequence)	XM_005168424.3
Accession Version	XM_005168424.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168424.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGAGG AGGTGATGAT TCACCGCCAG GTGATACTGA CCCAGAACTT GTACCAGAGC 
AGGTTTCTCG GGCTTGCAGC TATGGCCTCT CCATCTCGTG CATCTCAGGG CAGGAGGCGA 
TGTAAGGACC CGATCCGTCA CAGTTTTAAC CCTGAGCAGT TCCCTAATGT GGACTTCCAG 
AACGGCGTGG CGGAGGATCT GGCGGCGGCC GTGCTGCGCT CTACCAGCGA CACTGACCTG 
CTGACATCCC ACTGCCGCTC CACCCTCACC GTTAGCACCT CCAGCTACAC CATCGGTCAG 
ACGCAGGACA TCACCCTCAG CTGGGACATC AAGGAGGAGG TGGACGCCGG AGACTGGATT 
GGCATGTACC TCATTGATGA GGTTCTGTCA GAGAATTTCC TGGACTACAA AAACCGGGGT 
GTGAACGGCT CGCACAAAGG GCAGATTATC TGGAAGATTG AGGCCAGCTC CTATTTTGTG 
GAACCGGAGA CTAAGATCTG TTTTAAATAT TATCATGGAG TTAGTGGAGC GCTTCGGGCC 
ACAACACCAT GCGTGACTGT GAAGAACCCC TCTGCACCGG TGTTTAAGCC GGTGGCCACA 
GACGATGTTC CTCAGAGTCA GGGAAGAAGG AGACTCATCA GCTTCTCTCT GTCAGATTTC 
CAGGCCACCG GACTGAAAAA AGGCATGTTT TTTAACCCCG ACCCCTACCT AAAGATCGCT 
ATCCACCCTG GCAAACACAG CATCTTCCCA GCGCTGCCTC ACCATGGCCA GGAGAAGAGA 
TCCGGCATCG TCTGCAATAC CATTAATCCT GTTTGGCAGA GGGAGCGCTT TAATTTTGTG 
TCTCTGCCTA CGGATGTGTT GGAGATTGAG GTGAAGGACA AGTTCGCCAA AAGCCGTCCC 
ATCATAAAGC GTTTCTTGGG CAAGCTGTCC ATGCCAGTGC AAAGACTACT GGAGAAGCAT 
GCTATCGGTG ATCGAGTGGT CAGCTATACT CTGGGTCGAA GACTTCCAAC AGAGCATGTC 
AGCGGGCAGC TGCAGTTCAG GTTCGAGATC ACCTCCTCCA TTCATCCAGA TGATGAAGAC 
ATGTCTCTCA GCACAGAGCA GCCGGTGGCA GCAGCAGAAG CTCAAGCTCA GCCTCAAGCT 
GAAGACACCA TGAGTTTAGA TCTCGGAGCC CCGGAGCTGC CCTTAGATGG AGATACGGCT 
TCAGTCGAGA TGCCCATGGA TGTTGAATCT GGTCAGAGTT GTGCAGCTGA AGCAGATGTC 
ATAGAGGAAG AGTGTTTAGA GGAAGAACAG CAGGAAGGAG AAGCAGCAGC AGCAGCAGCA 
GCAATGGAGC AAAGTGAAAC CAATGCAGAA GAAACAACAC AAGTCTTGGA TCCCATCAAT 
GGCGCTGAAG AAGAAGAAGC AGAGAATAAT TGCGCCTTTG CAGAAGAGTC TTCAGATGCT 
CGCGCAGGAG AGGAAGATGA AGCGATTTCA GATCCGCAGC CAGCTTCAGA AGAGCCGGAT 
GCGGATTTAG AGGAAGACTG TTCACTGAGG ATCCGAACCA CTCCAAGAAG GAAACCCCGT 
CCCTGCTCTT TACCTGTCTC TGAGCTTGAG ACGGTCATTG CTTCAGCCTG TGGGGAACCT 
GAAACGCCAC GCTCTCATTA CATCCGAATA CACCACCTGC TCCACAGCCT GCCGGCCACT 
CAACCGCAGA CAGGAGACTC CGCCGGGCCT GAACCAGACC GACCAATGGG TGTAGAGGAG 
GAAGATGAAG AGGAAGAAGA AGAAGGTGTA ACTCTTGAAG ATCATGCTTT AACACCCAAT 
GGCCCTCGCA GGACTCTTCC TCGCAGCCGC TCTCACGAAC GCCTCTCTGA CCTCATTCAG 
ATGCTGGATG GAGATGGACA TCCGCTTGGG GCTGAGGGGC AAACCGGAAA TCAGAGGTCA 
CCTGATTTGA GCCCAGTGGC GTGCTGCAGT CACATGTCCC CGAGAGCTTC TGGTCCCATT 
CGGGCACAAT CCCTAGACAG TGCGCGGAGG TCAGAGAGTA CGGTGTTCTC CTCGCAGGAT 
GACGAGGACG AGATGGAGCA GCCAAATGGC ATTCTGGGAT CAGAGACGGA AACAGAGCAG 
GGCGCTCAAT GTCGGGAGCC AGGCGACGGG AGCTGTCAGT GGGAGGAGGC ACCGGACAGT 
CACGTTCAGA GCCCTCATGG CATTGTGGGT AATGGAGAAG TGGCCGGACC GAGCGGCAGA 
CTGAGTCAGC TGCCTGCCCT GCGGCCCGAT CCACACCACT ATCCCACCAT TGATGAGCCG 
CTGCCGCCCA ACTGGGAGGC CCGGATCGAC AGTCACGGTC GTGTGTTTTA TGTGGATCAC 
ATTAACCGGA CCACCACTTG GCAAAGACCC ACATCTGCAG CCACGCCGGA CGGCCTACGC 
AGGTCTGGAT CCGTCCAACA GATGGAGCAG CTCAACAGGA GGTATCAGAC AATCCAGAGG 
ACGATGGCCA CCGAGAGGAG AGAAGAGGAA TCAGGAAGCC CCCGTAATGA CAGAACAGCC 
AATACTGAGA CAGACTCACC TCTGCAAACA AACGAGTTGA GGAGAGACAC CTCATGTTCT 
CCAGGCCACT GTCAGAAGAT TACGTTACTG TTACAGAGTC CAGCGGTCAA ATTCATCACT 
CACCCAGAGT TCTTCACTGT TCTCCACGCT AATTATGGTG CATATCGAAT GTTCACCAAC 
AGCTCGTGTC TGAAACACAT GGTCCTGAAA ATTCGACGAG ATGCTCGAAA TTTTGAGCGC 
TACCAGCACA ATCGTGACCT GGTGACGTTT CTGAACAGGT TTGCAGACGC TCAGCTGGAG 
CTGCCGCGCG GCTGGGAGAT CAAAACTGAC CCGCAGGGGA AGTCATTTTT TGTAGACCAC 
AACAGTCGTG CCACAACCTT CATCGATCCC AGAATCCCCT TGCAAAACGG CAGATTACCA 
AACCACCTGA CGCACCGCCA GCACCTGCAG AGATTACGCA GCTACAGCGC AGGAGAGGCA 
TCTGAGGTGT CGCGCACCCG AGGGGTGTCT TTGTTAGCCA GGCCTGGAAA CAGCCTGGTA 
GCAGCCATAC GAAACCAAAG CCAGCAGGAG CCTGTGCCAT TAGCGTACAA TGATAAAATT 
GTAGCATTTT TACGGCAACC AAACATCTTC GAGGTGCTAC AGGAGCGTCA GCCCAGCTTT 
GCCAGGAATC ACTCACTCAA GGATAAAGTG CATCACATCC GAACCGAAGG CACACAGAGA 
CTGGAGAAGC TGTCATGTGA TGCTGACCTG GTCATGCTGT TAAGTTTGTT TGAGGAGGAC 
ATCATGTCTT ACGTTCCTCT CGCATCATTT CACCCCGGGT TCAACTTTTC ACCTCGCTGC 
TCTCCTGGTT CTTCTCCCCA GAACTCTCCA GGGACCCAGA GGGCTCGGGC TCCAGCGCCG 
TACAGAAGAG ACTTTGAGGC CAAATTGCGC AACTTCTACA GGAAGCTTGA GGCAAAGGGC 
TACGGGCAAG GGCCTGGGAA GATAAAATTG ATCGTCAGAA GAGATCATCT TTTAGAAGGA 
ACGTTTAACC AGGTCATGGC CTACTCACGT AAAGAGCTGC AGAGAAACAA GCTGTACATT 
ACCTTTGTGG GAGAGGAAGG TCTGGACTAC AGTGGCCCAT CGCGAGAGTT CTTCTTCCTG 
CTGTCTCAGG AGCTGTTTAA CCCTTACTAT GGCCTGTTTG AATACTCCGC CAACGACACA 
TACACAGTCC AGATCAGCCC CATGTCTGCT TTTGTGGAGA ACCACTTGGA ATGGTTCCGC 
TTCAGTGGTC GAATTCTCGG CCTGGCTTTG ATTCATCAGT ATTTGTTGGA CGCTTTCTTC 
ACTCGGCCAT TTTATAAAGC TCTTCTCAGA CTACCCACTG ATCTCAGCGA TCTGGAGTAT 
CTGGATGAGG AGTTTCACCA GAGCTTGCAG TGGATGAAGG AAAATGACAT CACTGATGTG 
CTGGACCTCA CCTTCACTGT CAATGAGGAA GTGTTCGGAC AGGTGACAGA GCGGGAGCTG 
AAGTCTGGCG GGACGAATGT GCAGGTGACG GAGAAAAATA AGAAGGAGTA CATTGAGCGG 
ATGGTGAAGT GGAGGGTGGA GCGAGGCGTT GTCCAGCAGA CACAGGCTCT GGTGCGAGGA 
TTTTATGAGG TGGTGGACTC TCGTCTGGTG TCTGTGTTTG ATGCCAGAGA GCTGGAGCTG 
GTCATCGCAG GAACAGCAGA GATCGATTTG AACGACTGGA GAAACAACAC AGAGTACAGA 
GGAGGTTATC ATGACGGGCA CATTGTGATC CGCTGGTTCT GGGGAGCAGT GGAGCGCTTC 
AATAATGAGC AGCGGCTGCG TCTGCTGCAG TTTGTGACGG GCACCTCCAG CGTGCCGTAT 
GAGGGCTTTA CCGCCCTACG TGGCAGCAAT GGACTGAGAC GCTTCTGCAT TGAGAAATGG 
GGAAAGATCA CATCACTCCC AAGGGCGCAC ACATGCTTTA ACCGTCTGGA TCTTCCACCA 
TATCCATCCT ACACGATGTT ATACGAGAAA CTCCTGATCG CTGTGGAAGA GACCAGCACT 
TTTGGCCTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168481.1
CDS	147..4721
Translation	MNEEVMIHRQVILTQNLYQSRFLGLAAMASPSRASQGRRRCKDPIRHSFNPEQFPNVDFQNGVAEDLAAAVLRSTSDTDLLTSHCRSTLTVSTSSYTIGQTQDITLSWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHKGQIIWKIEASSYFVEPETKICFKYYHGVSGALRATTPCVTVKNPSAPVFKPVATDDVPQSQGRRRLISFSLSDFQATGLKKGMFFNPDPYLKIAIHPGKHSIFPALPHHGQEKRSGIVCNTINPVWQRERFNFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLEKHAIGDRVVSYTLGRRLPTEHVSGQLQFRFEITSSIHPDDEDMSLSTEQPVAAAEAQAQPQAEDTMSLDLGAPELPLDGDTASVEMPMDVESGQSCAAEADVIEEECLEEEQQEGEAAAAAAAMEQSETNAEETTQVLDPINGAEEEEAENNCAFAEESSDARAGEEDEAISDPQPASEEPDADLEEDCSLRIRTTPRRKPRPCSLPVSELETVIASACGEPETPRSHYIRIHHLLHSLPATQPQTGDSAGPEPDRPMGVEEEDEEEEEEGVTLEDHALTPNGPRRTLPRSRSHERLSDLIQMLDGDGHPLGAEGQTGNQRSPDLSPVACCSHMSPRASGPIRAQSLDSARRSESTVFSSQDDEDEMEQPNGILGSETETEQGAQCREPGDGSCQWEEAPDSHVQSPHGIVGNGEVAGPSGRLSQLPALRPDPHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPTSAATPDGLRRSGSVQQMEQLNRRYQTIQRTMATERREEESGSPRNDRTANTETDSPLQTNELRRDTSCSPGHCQKITLLLQSPAVKFITHPEFFTVLHANYGAYRMFTNSSCLKHMVLKIRRDARNFERYQHNRDLVTFLNRFADAQLELPRGWEIKTDPQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRTRGVSLLARPGNSLVAAIRNQSQQEPVPLAYNDKIVAFLRQPNIFEVLQERQPSFARNHSLKDKVHHIRTEGTQRLEKLSCDADLVMLLSLFEEDIMSYVPLASFHPGFNFSPRCSPGSSPQNSPGTQRARAPAPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIVRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPTDLSDLEYLDEEFHQSLQWMKENDITDVLDLTFTVNEEVFGQVTERELKSGGTNVQVTEKNKKEYIERMVKWRVERGVVQQTQALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWGAVERFNNEQRLRLLQFVTGTSSVPYEGFTALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYTMLYEKLLIAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_005168424.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168424.3

ORF Insert Sequence:

ATGAACGAGG AGGTGATGAT TCACCGCCAG GTGATACTGA CCCAGAACTT GTACCAGAGC 
AGGTTTCTCG GGCTTGCAGC TATGGCCTCT CCATCTCGTG CATCTCAGGG CAGGAGGCGA 
TGTAAGGACC CGATCCGTCA CAGTTTTAAC CCTGAGCAGT TCCCTAATGT GGACTTCCAG 
AACGGCGTGG CGGAGGATCT GGCGGCGGCC GTGCTGCGCT CTACCAGCGA CACTGACCTG 
CTGACATCCC ACTGCCGCTC CACCCTCACC GTTAGCACCT CCAGCTACAC CATCGGTCAG 
ACGCAGGACA TCACCCTCAG CTGGGACATC AAGGAGGAGG TGGACGCCGG AGACTGGATT 
GGCATGTACC TCATTGATGA GGTTCTGTCA GAGAATTTCC TGGACTACAA AAACCGGGGT 
GTGAACGGCT CGCACAAAGG GCAGATTATC TGGAAGATTG AGGCCAGCTC CTATTTTGTG 
GAACCGGAGA CTAAGATCTG TTTTAAATAT TATCATGGAG TTAGTGGAGC GCTTCGGGCC 
ACAACACCAT GCGTGACTGT GAAGAACCCC TCTGCACCGG TGTTTAAGCC GGTGGCCACA 
GACGATGTTC CTCAGAGTCA GGGAAGAAGG AGACTCATCA GCTTCTCTCT GTCAGATTTC 
CAGGCCACCG GACTGAAAAA AGGCATGTTT TTTAACCCCG ACCCCTACCT AAAGATCGCT 
ATCCACCCTG GCAAACACAG CATCTTCCCA GCGCTGCCTC ACCATGGCCA GGAGAAGAGA 
TCCGGCATCG TCTGCAATAC CATTAATCCT GTTTGGCAGA GGGAGCGCTT TAATTTTGTG 
TCTCTGCCTA CGGATGTGTT GGAGATTGAG GTGAAGGACA AGTTCGCCAA AAGCCGTCCC 
ATCATAAAGC GTTTCTTGGG CAAGCTGTCC ATGCCAGTGC AAAGACTACT GGAGAAGCAT 
GCTATCGGTG ATCGAGTGGT CAGCTATACT CTGGGTCGAA GACTTCCAAC AGAGCATGTC 
AGCGGGCAGC TGCAGTTCAG GTTCGAGATC ACCTCCTCCA TTCATCCAGA TGATGAAGAC 
ATGTCTCTCA GCACAGAGCA GCCGGTGGCA GCAGCAGAAG CTCAAGCTCA GCCTCAAGCT 
GAAGACACCA TGAGTTTAGA TCTCGGAGCC CCGGAGCTGC CCTTAGATGG AGATACGGCT 
TCAGTCGAGA TGCCCATGGA TGTTGAATCT GGTCAGAGTT GTGCAGCTGA AGCAGATGTC 
ATAGAGGAAG AGTGTTTAGA GGAAGAACAG CAGGAAGGAG AAGCAGCAGC AGCAGCAGCA 
GCAATGGAGC AAAGTGAAAC CAATGCAGAA GAAACAACAC AAGTCTTGGA TCCCATCAAT 
GGCGCTGAAG AAGAAGAAGC AGAGAATAAT TGCGCCTTTG CAGAAGAGTC TTCAGATGCT 
CGCGCAGGAG AGGAAGATGA AGCGATTTCA GATCCGCAGC CAGCTTCAGA AGAGCCGGAT 
GCGGATTTAG AGGAAGACTG TTCACTGAGG ATCCGAACCA CTCCAAGAAG GAAACCCCGT 
CCCTGCTCTT TACCTGTCTC TGAGCTTGAG ACGGTCATTG CTTCAGCCTG TGGGGAACCT 
GAAACGCCAC GCTCTCATTA CATCCGAATA CACCACCTGC TCCACAGCCT GCCGGCCACT 
CAACCGCAGA CAGGAGACTC CGCCGGGCCT GAACCAGACC GACCAATGGG TGTAGAGGAG 
GAAGATGAAG AGGAAGAAGA AGAAGGTGTA ACTCTTGAAG ATCATGCTTT AACACCCAAT 
GGCCCTCGCA GGACTCTTCC TCGCAGCCGC TCTCACGAAC GCCTCTCTGA CCTCATTCAG 
ATGCTGGATG GAGATGGACA TCCGCTTGGG GCTGAGGGGC AAACCGGAAA TCAGAGGTCA 
CCTGATTTGA GCCCAGTGGC GTGCTGCAGT CACATGTCCC CGAGAGCTTC TGGTCCCATT 
CGGGCACAAT CCCTAGACAG TGCGCGGAGG TCAGAGAGTA CGGTGTTCTC CTCGCAGGAT 
GACGAGGACG AGATGGAGCA GCCAAATGGC ATTCTGGGAT CAGAGACGGA AACAGAGCAG 
GGCGCTCAAT GTCGGGAGCC AGGCGACGGG AGCTGTCAGT GGGAGGAGGC ACCGGACAGT 
CACGTTCAGA GCCCTCATGG CATTGTGGGT AATGGAGAAG TGGCCGGACC GAGCGGCAGA 
CTGAGTCAGC TGCCTGCCCT GCGGCCCGAT CCACACCACT ATCCCACCAT TGATGAGCCG 
CTGCCGCCCA ACTGGGAGGC CCGGATCGAC AGTCACGGTC GTGTGTTTTA TGTGGATCAC 
ATTAACCGGA CCACCACTTG GCAAAGACCC ACATCTGCAG CCACGCCGGA CGGCCTACGC 
AGGTCTGGAT CCGTCCAACA GATGGAGCAG CTCAACAGGA GGTATCAGAC AATCCAGAGG 
ACGATGGCCA CCGAGAGGAG AGAAGAGGAA TCAGGAAGCC CCCGTAATGA CAGAACAGCC 
AATACTGAGA CAGACTCACC TCTGCAAACA AACGAGTTGA GGAGAGACAC CTCATGTTCT 
CCAGGCCACT GTCAGAAGAT TACGTTACTG TTACAGAGTC CAGCGGTCAA ATTCATCACT 
CACCCAGAGT TCTTCACTGT TCTCCACGCT AATTATGGTG CATATCGAAT GTTCACCAAC 
AGCTCGTGTC TGAAACACAT GGTCCTGAAA ATTCGACGAG ATGCTCGAAA TTTTGAGCGC 
TACCAGCACA ATCGTGACCT GGTGACGTTT CTGAACAGGT TTGCAGACGC TCAGCTGGAG 
CTGCCGCGCG GCTGGGAGAT CAAAACTGAC CCGCAGGGGA AGTCATTTTT TGTAGACCAC 
AACAGTCGTG CCACAACCTT CATCGATCCC AGAATCCCCT TGCAAAACGG CAGATTACCA 
AACCACCTGA CGCACCGCCA GCACCTGCAG AGATTACGCA GCTACAGCGC AGGAGAGGCA 
TCTGAGGTGT CGCGCACCCG AGGGGTGTCT TTGTTAGCCA GGCCTGGAAA CAGCCTGGTA 
GCAGCCATAC GAAACCAAAG CCAGCAGGAG CCTGTGCCAT TAGCGTACAA TGATAAAATT 
GTAGCATTTT TACGGCAACC AAACATCTTC GAGGTGCTAC AGGAGCGTCA GCCCAGCTTT 
GCCAGGAATC ACTCACTCAA GGATAAAGTG CATCACATCC GAACCGAAGG CACACAGAGA 
CTGGAGAAGC TGTCATGTGA TGCTGACCTG GTCATGCTGT TAAGTTTGTT TGAGGAGGAC 
ATCATGTCTT ACGTTCCTCT CGCATCATTT CACCCCGGGT TCAACTTTTC ACCTCGCTGC 
TCTCCTGGTT CTTCTCCCCA GAACTCTCCA GGGACCCAGA GGGCTCGGGC TCCAGCGCCG 
TACAGAAGAG ACTTTGAGGC CAAATTGCGC AACTTCTACA GGAAGCTTGA GGCAAAGGGC 
TACGGGCAAG GGCCTGGGAA GATAAAATTG ATCGTCAGAA GAGATCATCT TTTAGAAGGA 
ACGTTTAACC AGGTCATGGC CTACTCACGT AAAGAGCTGC AGAGAAACAA GCTGTACATT 
ACCTTTGTGG GAGAGGAAGG TCTGGACTAC AGTGGCCCAT CGCGAGAGTT CTTCTTCCTG 
CTGTCTCAGG AGCTGTTTAA CCCTTACTAT GGCCTGTTTG AATACTCCGC CAACGACACA 
TACACAGTCC AGATCAGCCC CATGTCTGCT TTTGTGGAGA ACCACTTGGA ATGGTTCCGC 
TTCAGTGGTC GAATTCTCGG CCTGGCTTTG ATTCATCAGT ATTTGTTGGA CGCTTTCTTC 
ACTCGGCCAT TTTATAAAGC TCTTCTCAGA CTACCCACTG ATCTCAGCGA TCTGGAGTAT 
CTGGATGAGG AGTTTCACCA GAGCTTGCAG TGGATGAAGG AAAATGACAT CACTGATGTG 
CTGGACCTCA CCTTCACTGT CAATGAGGAA GTGTTCGGAC AGGTGACAGA GCGGGAGCTG 
AAGTCTGGCG GGACGAATGT GCAGGTGACG GAGAAAAATA AGAAGGAGTA CATTGAGCGG 
ATGGTGAAGT GGAGGGTGGA GCGAGGCGTT GTCCAGCAGA CACAGGCTCT GGTGCGAGGA 
TTTTATGAGG TGGTGGACTC TCGTCTGGTG TCTGTGTTTG ATGCCAGAGA GCTGGAGCTG 
GTCATCGCAG GAACAGCAGA GATCGATTTG AACGACTGGA GAAACAACAC AGAGTACAGA 
GGAGGTTATC ATGACGGGCA CATTGTGATC CGCTGGTTCT GGGGAGCAGT GGAGCGCTTC 
AATAATGAGC AGCGGCTGCG TCTGCTGCAG TTTGTGACGG GCACCTCCAG CGTGCCGTAT 
GAGGGCTTTA CCGCCCTACG TGGCAGCAAT GGACTGAGAC GCTTCTGCAT TGAGAAATGG 
GGAAAGATCA CATCACTCCC AAGGGCGCAC ACATGCTTTA ACCGTCTGGA TCTTCCACCA 
TATCCATCCT ACACGATGTT ATACGAGAAA CTCCTGATCG CTGTGGAAGA GACCAGCACT 
TTTGGCCTTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hecw1b ( XM_009298865.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297140.1 Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), transcript variant X3, mRNA.

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CloneID	ODa44163
Clone ID Related Accession (Same CDS sequence)	XM_009298865.3 , XM_009298865.2
Accession Version	XM_009298865.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298865.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGAGG AGGTGATGAT TCACCGCCAG GTGATACTGA CCCAGAACTT GTACCAGAGC 
AGGTTTCTCG GGCTTGCAGC TATGGCCTCT CCATCTCGTG CATCTCAGGG CAGGAGGCGA 
TGTAAGGACC CGATCCGTCA CAGTTTTAAC CCTGAGCAGT TCCCTAATGT GGACTTCCAG 
AACGGCGTGG CGGAGGATCT GGCGGCGGCC GTGCTGCGCT CTACCAGCGA CACTGACCTG 
CTGACATCCC ACTGCCGCTC CACCCTCACC GTTAGCACCT CCAGCTACAC CATCGGTCAG 
ACGCAGGACA TCACCCTCAG CTGGGACATC AAGGAGGAGG TGGACGCCGG AGACTGGATT 
GGCATGTACC TCATTGATGA GGTTCTGTCA GAGAATTTCC TGGACTACAA AAACCGGGGT 
GTGAACGGCT CGCACAAAGG GCAGATTATC TGGAAGATTG AGGCCAGCTC CTATTTTGTG 
GAACCGGAGA CTAAGATCTG TTTTAAATAT TATCATGGAG TTAGTGGAGC GCTTCGGGCC 
ACAACACCAT GCGTGACTGT GAAGAACCCC TCTGCACCGG TGTTTAAGCC GGTGGCCACA 
GACGATGTTC CTCAGAGTCA GGGAAGAAGG AGACTCATCA GCTTCTCTCT GTCAGATTTC 
CAGGCCACCG GACTGAAAAA AGGCATGTTT TTTAACCCCG ACCCCTACCT AAAGATCGCT 
ATCCACCCTG GCAAACACAG CATCTTCCCA GCGCTGCCTC ACCATGGCCA GGAGAAGAGA 
TCCGGCATCG TCTGCAATAC CATTAATCCT GTTTGGCAGA GGGAGCGCTT TAATTTTGTG 
TCTCTGCCTA CGGATGTGTT GGAGATTGAG GTGAAGGACA AGTTCGCCAA AAGCCGTCCC 
ATCATAAAGC GTTTCTTGGG CAAGCTGTCC ATGCCAGTGC AAAGACTACT GGAGAAGCAT 
GCTATCGGTG ATCGAGTGGT CAGCTATACT CTGGGTCGAA GACTTCCAAC AGAGCATGTC 
AGCGGGCAGC TGCAGTTCAG GTTCGAGATC ACCTCCTCCA TTCATCCAGA TGATGAAGAC 
ATGTCTCTCA GCACAGAGCA GCCGGTGGCA GCAGCAGAAG CTCAAGCTCA GCCTCAAGCT 
GAAGACACCA TGAGTTTAGA TCTCGGAGCC CCGGAGCTGC CCTTAGATGG AGATACGGCT 
TCAGTCGAGA TGCCCATGGA TGTTGAATCT GGTCAGAGTT GTGCAGCTGA AGCAGATGTC 
ATAGAGGAAG AGTGTTTAGA GGAAGAACAG CAGGAAGGAG AAGCAGCAGC AGCAGCAGCA 
GCAATGGAGC AAAGTGAAAC CAATGCAGAA GAAACAACAC AAGTCTTGGA TCCCATCAAT 
GGCGCTGAAG AAGAAGAAGC AGAGAATAAT TGCGCCTTTG CAGAAGAGTC TTCAGATGCT 
CGCGCAGGAG AGGAAGATGA AGCGATTTCA GATCCGCAGC CAGCTTCAGA AGAGCCGGAT 
GCGGATTTAG AGGAAGACTG TTCACTGAGG ATCCGAACCA CTCCAAGAAG GAAACCCCGT 
CCCTGCTCTT TACCTGTCTC TGAGCTTGAG ACGGTCATTG CTTCAGCCTG TGGGGAACCT 
GAAACGCCAC GCTCTCATTA CATCCGAATA CACCACCTGC TCCACAGCCT GCCGGCCACT 
CAACCGCAGA CAGGAGACTC CGCCGGGCCT GAACCAGACC GACCAATGGG TGTAGAGGAG 
GAAGATGAAG AGGAAGAAGA AGAAGGTGTA ACTCTTGAAG ATCATGCTTT AACACCCAAT 
GGCCCTCGCA GGACTCTTCC TCGCAGCCGC TCTCACGAAC GCCTCTCTGA CCTCATTCAG 
ATGCTGGATG GAGATGGACA TCCGCTTGGG GCTGAGGGGC AAACCGGAAA TCAGAGGTCA 
CCTGATTTGA GCCCAGTGGC GTGCTGCAGT CACATGTCCC CGAGAGCTTC TGGTCCCATT 
CGGGCACAAT CCCTAGACAG TGCGCGGAGG TCAGAGAGTA CGGTGTTCTC CTCGCAGGAT 
GACGAGGACG AGATGGAGCA GCCAAATGGC ATTCTGGGAT CAGAGACGGA AACAGAGCAG 
GGCGCTCAAT GTCGGGAGCC AGGCGACGGG AGCTGTCAGT GGGAGGAGGC ACCGGACAGT 
CACGTTCAGA GCCCTCATGG CATTGTGGGT AATGGAGAAG TGGCCGGACC GAGCGGCAGA 
CGAGAATGTC CTTTGCCTTG TAACCACCCT GCAGTGAGTC AGCTGCCTGC CCTGCGGCCC 
GATCCACACC ACTATCCCAC CATTGATGAG CCGCTGCCGC CCAACTGGGA GGCCCGGATC 
GACAGTCACG GTCGTGTGTT TTATGTGGAT CACATTAACC GGACCACCAC TTGGCAAAGA 
CCCACATCTG CAGCCACGCC GGACGGCCTA CGCAGGTCTG GATCCGTCCA ACAGATGGAG 
CAGCTCAACA GGAGGTATCA GACAATCCAG AGGACGATGG CCACCGAGAG GAGAGAAGAG 
GAATCAGGAA GCCCCCGTAA TGACAGAACA GCCAATACTG AGACAGACTC ACCTCTGCAA 
ACAAACGAGT TGAGGAGAGA CACCTCATGT TCTCCAGGCC ACTGTCAGAA GATTACGTTA 
CTGTTACAGA GTCCAGCGGT CAAATTCATC ACTCACCCAG AGTTCTTCAC TGTTCTCCAC 
GCTAATTATG GTGCATATCG AATGTTCACC AACAGCTCGT GTCTGAAACA CATGGTCCTG 
AAAATTCGAC GAGATGCTCG AAATTTTGAG CGCTACCAGC ACAATCGTGA CCTGGTGACG 
TTTCTGAACA GGTTTGCAGA CGCTCAGCTG GAGCTGCCGC GCGGCTGGGA GATCAAAACT 
GACCCGCAGG GGAAGTCATT TTTTGTAGAC CACAACAGTC GTGCCACAAC CTTCATCGAT 
CCCAGAATCC CCTTGCAAAA CGGCAGATTA CCAAACCACC TGACGCACCG CCAGCACCTG 
CAGAGATTAC GCAGCTACAG CGCAGGAGAG GCATCTGAGG TGTCGCGCAC CCGAGGGGTG 
TCTTTGTTAG CCAGGCCTGG AAACAGCCTG GTAGCAGCCA TACGAAACCA AAGCCAGCAG 
GAGCCTGTGC CATTAGCGTA CAATGATAAA ATTGTAGCAT TTTTACGGCA ACCAAACATC 
TTCGAGGTGC TACAGGAGCG TCAGCCCAGC TTTGCCAGGA ATCACTCACT CAAGGATAAA 
GTGCATCACA TCCGAACCGA AGGCACACAG AGACTGGAGA AGCTGTCATG TGATGCTGAC 
CTGGTCATGC TGTTAAGTTT GTTTGAGGAG GACATCATGT CTTACGTTCC TCTCGCATCA 
TTTCACCCCG GGTTCAACTT TTCACCTCGC TGCTCTCCTG GTTCTTCTCC CCAGAACTCT 
CCAGGGACCC AGAGGGCTCG GGCTCCAGCG CCGTACAGAA GAGACTTTGA GGCCAAATTG 
CGCAACTTCT ACAGGAAGCT TGAGGCAAAG GGCTACGGGC AAGGGCCTGG GAAGATAAAA 
TTGATCGTCA GAAGAGATCA TCTTTTAGAA GGAACGTTTA ACCAGGTCAT GGCCTACTCA 
CGTAAAGAGC TGCAGAGAAA CAAGCTGTAC ATTACCTTTG TGGGAGAGGA AGGTCTGGAC 
TACAGTGGCC CATCGCGAGA GTTCTTCTTC CTGCTGTCTC AGGAGCTGTT TAACCCTTAC 
TATGGCCTGT TTGAATACTC CGCCAACGAC ACATACACAG TCCAGATCAG CCCCATGTCT 
GCTTTTGTGG AGAACCACTT GGAATGGTTC CGCTTCAGTG GTCGAATTCT CGGCCTGGCT 
TTGATTCATC AGTATTTGTT GGACGCTTTC TTCACTCGGC CATTTTATAA AGCTCTTCTC 
AGACTACCCA CTGATCTCAG CGATCTGGAG TATCTGGATG AGGAGTTTCA CCAGAGCTTG 
CAGTGGATGA AGGAAAATGA CATCACTGAT GTGCTGGACC TCACCTTCAC TGTCAATGAG 
GAAGTGTTCG GACAGGTGAC AGAGCGGGAG CTGAAGTCTG GCGGGACGAA TGTGCAGGTG 
ACGGAGAAAA ATAAGAAGGA GTACATTGAG CGGATGGTGA AGTGGAGGGT GGAGCGAGGC 
GTTGTCCAGC AGACACAGGC TCTGGTGCGA GGATTTTATG AGGTGGTGGA CTCTCGTCTG 
GTGTCTGTGT TTGATGCCAG AGAGCTGGAG CTGGTCATCG CAGGAACAGC AGAGATCGAT 
TTGAACGACT GGAGAAACAA CACAGAGTAC AGAGGAGGTT ATCATGACGG GCACATTGTG 
ATCCGCTGGT TCTGGGGAGC AGTGGAGCGC TTCAATAATG AGCAGCGGCT GCGTCTGCTG 
CAGTTTGTGA CGGGCACCTC CAGCGTGCCG TATGAGGGCT TTACCGCCCT ACGTGGCAGC 
AATGGACTGA GACGCTTCTG CATTGAGAAA TGGGGAAAGA TCACATCACT CCCAAGGGCG 
CACACATGCT TTAACCGTCT GGATCTTCCA CCATATCCAT CCTACACGAT GTTATACGAG 
AAACTCCTGA TCGCTGTGGA AGAGACCAGC ACTTTTGGCC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297140.1
CDS	152..4759
Translation	MNEEVMIHRQVILTQNLYQSRFLGLAAMASPSRASQGRRRCKDPIRHSFNPEQFPNVDFQNGVAEDLAAAVLRSTSDTDLLTSHCRSTLTVSTSSYTIGQTQDITLSWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHKGQIIWKIEASSYFVEPETKICFKYYHGVSGALRATTPCVTVKNPSAPVFKPVATDDVPQSQGRRRLISFSLSDFQATGLKKGMFFNPDPYLKIAIHPGKHSIFPALPHHGQEKRSGIVCNTINPVWQRERFNFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLEKHAIGDRVVSYTLGRRLPTEHVSGQLQFRFEITSSIHPDDEDMSLSTEQPVAAAEAQAQPQAEDTMSLDLGAPELPLDGDTASVEMPMDVESGQSCAAEADVIEEECLEEEQQEGEAAAAAAAMEQSETNAEETTQVLDPINGAEEEEAENNCAFAEESSDARAGEEDEAISDPQPASEEPDADLEEDCSLRIRTTPRRKPRPCSLPVSELETVIASACGEPETPRSHYIRIHHLLHSLPATQPQTGDSAGPEPDRPMGVEEEDEEEEEEGVTLEDHALTPNGPRRTLPRSRSHERLSDLIQMLDGDGHPLGAEGQTGNQRSPDLSPVACCSHMSPRASGPIRAQSLDSARRSESTVFSSQDDEDEMEQPNGILGSETETEQGAQCREPGDGSCQWEEAPDSHVQSPHGIVGNGEVAGPSGRRECPLPCNHPAVSQLPALRPDPHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPTSAATPDGLRRSGSVQQMEQLNRRYQTIQRTMATERREEESGSPRNDRTANTETDSPLQTNELRRDTSCSPGHCQKITLLLQSPAVKFITHPEFFTVLHANYGAYRMFTNSSCLKHMVLKIRRDARNFERYQHNRDLVTFLNRFADAQLELPRGWEIKTDPQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRTRGVSLLARPGNSLVAAIRNQSQQEPVPLAYNDKIVAFLRQPNIFEVLQERQPSFARNHSLKDKVHHIRTEGTQRLEKLSCDADLVMLLSLFEEDIMSYVPLASFHPGFNFSPRCSPGSSPQNSPGTQRARAPAPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIVRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPTDLSDLEYLDEEFHQSLQWMKENDITDVLDLTFTVNEEVFGQVTERELKSGGTNVQVTEKNKKEYIERMVKWRVERGVVQQTQALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWGAVERFNNEQRLRLLQFVTGTSSVPYEGFTALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYTMLYEKLLIAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009298865.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298865.3

ORF Insert Sequence:

ATGAACGAGG AGGTGATGAT TCACCGCCAG GTGATACTGA CCCAGAACTT GTACCAGAGC 
AGGTTTCTCG GGCTTGCAGC TATGGCCTCT CCATCTCGTG CATCTCAGGG CAGGAGGCGA 
TGTAAGGACC CGATCCGTCA CAGTTTTAAC CCTGAGCAGT TCCCTAATGT GGACTTCCAG 
AACGGCGTGG CGGAGGATCT GGCGGCGGCC GTGCTGCGCT CTACCAGCGA CACTGACCTG 
CTGACATCCC ACTGCCGCTC CACCCTCACC GTTAGCACCT CCAGCTACAC CATCGGTCAG 
ACGCAGGACA TCACCCTCAG CTGGGACATC AAGGAGGAGG TGGACGCCGG AGACTGGATT 
GGCATGTACC TCATTGATGA GGTTCTGTCA GAGAATTTCC TGGACTACAA AAACCGGGGT 
GTGAACGGCT CGCACAAAGG GCAGATTATC TGGAAGATTG AGGCCAGCTC CTATTTTGTG 
GAACCGGAGA CTAAGATCTG TTTTAAATAT TATCATGGAG TTAGTGGAGC GCTTCGGGCC 
ACAACACCAT GCGTGACTGT GAAGAACCCC TCTGCACCGG TGTTTAAGCC GGTGGCCACA 
GACGATGTTC CTCAGAGTCA GGGAAGAAGG AGACTCATCA GCTTCTCTCT GTCAGATTTC 
CAGGCCACCG GACTGAAAAA AGGCATGTTT TTTAACCCCG ACCCCTACCT AAAGATCGCT 
ATCCACCCTG GCAAACACAG CATCTTCCCA GCGCTGCCTC ACCATGGCCA GGAGAAGAGA 
TCCGGCATCG TCTGCAATAC CATTAATCCT GTTTGGCAGA GGGAGCGCTT TAATTTTGTG 
TCTCTGCCTA CGGATGTGTT GGAGATTGAG GTGAAGGACA AGTTCGCCAA AAGCCGTCCC 
ATCATAAAGC GTTTCTTGGG CAAGCTGTCC ATGCCAGTGC AAAGACTACT GGAGAAGCAT 
GCTATCGGTG ATCGAGTGGT CAGCTATACT CTGGGTCGAA GACTTCCAAC AGAGCATGTC 
AGCGGGCAGC TGCAGTTCAG GTTCGAGATC ACCTCCTCCA TTCATCCAGA TGATGAAGAC 
ATGTCTCTCA GCACAGAGCA GCCGGTGGCA GCAGCAGAAG CTCAAGCTCA GCCTCAAGCT 
GAAGACACCA TGAGTTTAGA TCTCGGAGCC CCGGAGCTGC CCTTAGATGG AGATACGGCT 
TCAGTCGAGA TGCCCATGGA TGTTGAATCT GGTCAGAGTT GTGCAGCTGA AGCAGATGTC 
ATAGAGGAAG AGTGTTTAGA GGAAGAACAG CAGGAAGGAG AAGCAGCAGC AGCAGCAGCA 
GCAATGGAGC AAAGTGAAAC CAATGCAGAA GAAACAACAC AAGTCTTGGA TCCCATCAAT 
GGCGCTGAAG AAGAAGAAGC AGAGAATAAT TGCGCCTTTG CAGAAGAGTC TTCAGATGCT 
CGCGCAGGAG AGGAAGATGA AGCGATTTCA GATCCGCAGC CAGCTTCAGA AGAGCCGGAT 
GCGGATTTAG AGGAAGACTG TTCACTGAGG ATCCGAACCA CTCCAAGAAG GAAACCCCGT 
CCCTGCTCTT TACCTGTCTC TGAGCTTGAG ACGGTCATTG CTTCAGCCTG TGGGGAACCT 
GAAACGCCAC GCTCTCATTA CATCCGAATA CACCACCTGC TCCACAGCCT GCCGGCCACT 
CAACCGCAGA CAGGAGACTC CGCCGGGCCT GAACCAGACC GACCAATGGG TGTAGAGGAG 
GAAGATGAAG AGGAAGAAGA AGAAGGTGTA ACTCTTGAAG ATCATGCTTT AACACCCAAT 
GGCCCTCGCA GGACTCTTCC TCGCAGCCGC TCTCACGAAC GCCTCTCTGA CCTCATTCAG 
ATGCTGGATG GAGATGGACA TCCGCTTGGG GCTGAGGGGC AAACCGGAAA TCAGAGGTCA 
CCTGATTTGA GCCCAGTGGC GTGCTGCAGT CACATGTCCC CGAGAGCTTC TGGTCCCATT 
CGGGCACAAT CCCTAGACAG TGCGCGGAGG TCAGAGAGTA CGGTGTTCTC CTCGCAGGAT 
GACGAGGACG AGATGGAGCA GCCAAATGGC ATTCTGGGAT CAGAGACGGA AACAGAGCAG 
GGCGCTCAAT GTCGGGAGCC AGGCGACGGG AGCTGTCAGT GGGAGGAGGC ACCGGACAGT 
CACGTTCAGA GCCCTCATGG CATTGTGGGT AATGGAGAAG TGGCCGGACC GAGCGGCAGA 
CGAGAATGTC CTTTGCCTTG TAACCACCCT GCAGTGAGTC AGCTGCCTGC CCTGCGGCCC 
GATCCACACC ACTATCCCAC CATTGATGAG CCGCTGCCGC CCAACTGGGA GGCCCGGATC 
GACAGTCACG GTCGTGTGTT TTATGTGGAT CACATTAACC GGACCACCAC TTGGCAAAGA 
CCCACATCTG CAGCCACGCC GGACGGCCTA CGCAGGTCTG GATCCGTCCA ACAGATGGAG 
CAGCTCAACA GGAGGTATCA GACAATCCAG AGGACGATGG CCACCGAGAG GAGAGAAGAG 
GAATCAGGAA GCCCCCGTAA TGACAGAACA GCCAATACTG AGACAGACTC ACCTCTGCAA 
ACAAACGAGT TGAGGAGAGA CACCTCATGT TCTCCAGGCC ACTGTCAGAA GATTACGTTA 
CTGTTACAGA GTCCAGCGGT CAAATTCATC ACTCACCCAG AGTTCTTCAC TGTTCTCCAC 
GCTAATTATG GTGCATATCG AATGTTCACC AACAGCTCGT GTCTGAAACA CATGGTCCTG 
AAAATTCGAC GAGATGCTCG AAATTTTGAG CGCTACCAGC ACAATCGTGA CCTGGTGACG 
TTTCTGAACA GGTTTGCAGA CGCTCAGCTG GAGCTGCCGC GCGGCTGGGA GATCAAAACT 
GACCCGCAGG GGAAGTCATT TTTTGTAGAC CACAACAGTC GTGCCACAAC CTTCATCGAT 
CCCAGAATCC CCTTGCAAAA CGGCAGATTA CCAAACCACC TGACGCACCG CCAGCACCTG 
CAGAGATTAC GCAGCTACAG CGCAGGAGAG GCATCTGAGG TGTCGCGCAC CCGAGGGGTG 
TCTTTGTTAG CCAGGCCTGG AAACAGCCTG GTAGCAGCCA TACGAAACCA AAGCCAGCAG 
GAGCCTGTGC CATTAGCGTA CAATGATAAA ATTGTAGCAT TTTTACGGCA ACCAAACATC 
TTCGAGGTGC TACAGGAGCG TCAGCCCAGC TTTGCCAGGA ATCACTCACT CAAGGATAAA 
GTGCATCACA TCCGAACCGA AGGCACACAG AGACTGGAGA AGCTGTCATG TGATGCTGAC 
CTGGTCATGC TGTTAAGTTT GTTTGAGGAG GACATCATGT CTTACGTTCC TCTCGCATCA 
TTTCACCCCG GGTTCAACTT TTCACCTCGC TGCTCTCCTG GTTCTTCTCC CCAGAACTCT 
CCAGGGACCC AGAGGGCTCG GGCTCCAGCG CCGTACAGAA GAGACTTTGA GGCCAAATTG 
CGCAACTTCT ACAGGAAGCT TGAGGCAAAG GGCTACGGGC AAGGGCCTGG GAAGATAAAA 
TTGATCGTCA GAAGAGATCA TCTTTTAGAA GGAACGTTTA ACCAGGTCAT GGCCTACTCA 
CGTAAAGAGC TGCAGAGAAA CAAGCTGTAC ATTACCTTTG TGGGAGAGGA AGGTCTGGAC 
TACAGTGGCC CATCGCGAGA GTTCTTCTTC CTGCTGTCTC AGGAGCTGTT TAACCCTTAC 
TATGGCCTGT TTGAATACTC CGCCAACGAC ACATACACAG TCCAGATCAG CCCCATGTCT 
GCTTTTGTGG AGAACCACTT GGAATGGTTC CGCTTCAGTG GTCGAATTCT CGGCCTGGCT 
TTGATTCATC AGTATTTGTT GGACGCTTTC TTCACTCGGC CATTTTATAA AGCTCTTCTC 
AGACTACCCA CTGATCTCAG CGATCTGGAG TATCTGGATG AGGAGTTTCA CCAGAGCTTG 
CAGTGGATGA AGGAAAATGA CATCACTGAT GTGCTGGACC TCACCTTCAC TGTCAATGAG 
GAAGTGTTCG GACAGGTGAC AGAGCGGGAG CTGAAGTCTG GCGGGACGAA TGTGCAGGTG 
ACGGAGAAAA ATAAGAAGGA GTACATTGAG CGGATGGTGA AGTGGAGGGT GGAGCGAGGC 
GTTGTCCAGC AGACACAGGC TCTGGTGCGA GGATTTTATG AGGTGGTGGA CTCTCGTCTG 
GTGTCTGTGT TTGATGCCAG AGAGCTGGAG CTGGTCATCG CAGGAACAGC AGAGATCGAT 
TTGAACGACT GGAGAAACAA CACAGAGTAC AGAGGAGGTT ATCATGACGG GCACATTGTG 
ATCCGCTGGT TCTGGGGAGC AGTGGAGCGC TTCAATAATG AGCAGCGGCT GCGTCTGCTG 
CAGTTTGTGA CGGGCACCTC CAGCGTGCCG TATGAGGGCT TTACCGCCCT ACGTGGCAGC 
AATGGACTGA GACGCTTCTG CATTGAGAAA TGGGGAAAGA TCACATCACT CCCAAGGGCG 
CACACATGCT TTAACCGTCT GGATCTTCCA CCATATCCAT CCTACACGAT GTTATACGAG 
AAACTCCTGA TCGCTGTGGA AGAGACCAGC ACTTTTGGCC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hecw1b ( XM_009298865.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297140.1 Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), transcript variant X1, mRNA.

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CloneID	ODa44163
Clone ID Related Accession (Same CDS sequence)	XM_009298865.3 , XM_009298865.2
Accession Version	XM_009298865.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298865.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGAGG AGGTGATGAT TCACCGCCAG GTGATACTGA CCCAGAACTT GTACCAGAGC 
AGGTTTCTCG GGCTTGCAGC TATGGCCTCT CCATCTCGTG CATCTCAGGG CAGGAGGCGA 
TGTAAGGACC CGATCCGTCA CAGTTTTAAC CCTGAGCAGT TCCCTAATGT GGACTTCCAG 
AACGGCGTGG CGGAGGATCT GGCGGCGGCC GTGCTGCGCT CTACCAGCGA CACTGACCTG 
CTGACATCCC ACTGCCGCTC CACCCTCACC GTTAGCACCT CCAGCTACAC CATCGGTCAG 
ACGCAGGACA TCACCCTCAG CTGGGACATC AAGGAGGAGG TGGACGCCGG AGACTGGATT 
GGCATGTACC TCATTGATGA GGTTCTGTCA GAGAATTTCC TGGACTACAA AAACCGGGGT 
GTGAACGGCT CGCACAAAGG GCAGATTATC TGGAAGATTG AGGCCAGCTC CTATTTTGTG 
GAACCGGAGA CTAAGATCTG TTTTAAATAT TATCATGGAG TTAGTGGAGC GCTTCGGGCC 
ACAACACCAT GCGTGACTGT GAAGAACCCC TCTGCACCGG TGTTTAAGCC GGTGGCCACA 
GACGATGTTC CTCAGAGTCA GGGAAGAAGG AGACTCATCA GCTTCTCTCT GTCAGATTTC 
CAGGCCACCG GACTGAAAAA AGGCATGTTT TTTAACCCCG ACCCCTACCT AAAGATCGCT 
ATCCACCCTG GCAAACACAG CATCTTCCCA GCGCTGCCTC ACCATGGCCA GGAGAAGAGA 
TCCGGCATCG TCTGCAATAC CATTAATCCT GTTTGGCAGA GGGAGCGCTT TAATTTTGTG 
TCTCTGCCTA CGGATGTGTT GGAGATTGAG GTGAAGGACA AGTTCGCCAA AAGCCGTCCC 
ATCATAAAGC GTTTCTTGGG CAAGCTGTCC ATGCCAGTGC AAAGACTACT GGAGAAGCAT 
GCTATCGGTG ATCGAGTGGT CAGCTATACT CTGGGTCGAA GACTTCCAAC AGAGCATGTC 
AGCGGGCAGC TGCAGTTCAG GTTCGAGATC ACCTCCTCCA TTCATCCAGA TGATGAAGAC 
ATGTCTCTCA GCACAGAGCA GCCGGTGGCA GCAGCAGAAG CTCAAGCTCA GCCTCAAGCT 
GAAGACACCA TGAGTTTAGA TCTCGGAGCC CCGGAGCTGC CCTTAGATGG AGATACGGCT 
TCAGTCGAGA TGCCCATGGA TGTTGAATCT GGTCAGAGTT GTGCAGCTGA AGCAGATGTC 
ATAGAGGAAG AGTGTTTAGA GGAAGAACAG CAGGAAGGAG AAGCAGCAGC AGCAGCAGCA 
GCAATGGAGC AAAGTGAAAC CAATGCAGAA GAAACAACAC AAGTCTTGGA TCCCATCAAT 
GGCGCTGAAG AAGAAGAAGC AGAGAATAAT TGCGCCTTTG CAGAAGAGTC TTCAGATGCT 
CGCGCAGGAG AGGAAGATGA AGCGATTTCA GATCCGCAGC CAGCTTCAGA AGAGCCGGAT 
GCGGATTTAG AGGAAGACTG TTCACTGAGG ATCCGAACCA CTCCAAGAAG GAAACCCCGT 
CCCTGCTCTT TACCTGTCTC TGAGCTTGAG ACGGTCATTG CTTCAGCCTG TGGGGAACCT 
GAAACGCCAC GCTCTCATTA CATCCGAATA CACCACCTGC TCCACAGCCT GCCGGCCACT 
CAACCGCAGA CAGGAGACTC CGCCGGGCCT GAACCAGACC GACCAATGGG TGTAGAGGAG 
GAAGATGAAG AGGAAGAAGA AGAAGGTGTA ACTCTTGAAG ATCATGCTTT AACACCCAAT 
GGCCCTCGCA GGACTCTTCC TCGCAGCCGC TCTCACGAAC GCCTCTCTGA CCTCATTCAG 
ATGCTGGATG GAGATGGACA TCCGCTTGGG GCTGAGGGGC AAACCGGAAA TCAGAGGTCA 
CCTGATTTGA GCCCAGTGGC GTGCTGCAGT CACATGTCCC CGAGAGCTTC TGGTCCCATT 
CGGGCACAAT CCCTAGACAG TGCGCGGAGG TCAGAGAGTA CGGTGTTCTC CTCGCAGGAT 
GACGAGGACG AGATGGAGCA GCCAAATGGC ATTCTGGGAT CAGAGACGGA AACAGAGCAG 
GGCGCTCAAT GTCGGGAGCC AGGCGACGGG AGCTGTCAGT GGGAGGAGGC ACCGGACAGT 
CACGTTCAGA GCCCTCATGG CATTGTGGGT AATGGAGAAG TGGCCGGACC GAGCGGCAGA 
CGAGAATGTC CTTTGCCTTG TAACCACCCT GCAGTGAGTC AGCTGCCTGC CCTGCGGCCC 
GATCCACACC ACTATCCCAC CATTGATGAG CCGCTGCCGC CCAACTGGGA GGCCCGGATC 
GACAGTCACG GTCGTGTGTT TTATGTGGAT CACATTAACC GGACCACCAC TTGGCAAAGA 
CCCACATCTG CAGCCACGCC GGACGGCCTA CGCAGGTCTG GATCCGTCCA ACAGATGGAG 
CAGCTCAACA GGAGGTATCA GACAATCCAG AGGACGATGG CCACCGAGAG GAGAGAAGAG 
GAATCAGGAA GCCCCCGTAA TGACAGAACA GCCAATACTG AGACAGACTC ACCTCTGCAA 
ACAAACGAGT TGAGGAGAGA CACCTCATGT TCTCCAGGCC ACTGTCAGAA GATTACGTTA 
CTGTTACAGA GTCCAGCGGT CAAATTCATC ACTCACCCAG AGTTCTTCAC TGTTCTCCAC 
GCTAATTATG GTGCATATCG AATGTTCACC AACAGCTCGT GTCTGAAACA CATGGTCCTG 
AAAATTCGAC GAGATGCTCG AAATTTTGAG CGCTACCAGC ACAATCGTGA CCTGGTGACG 
TTTCTGAACA GGTTTGCAGA CGCTCAGCTG GAGCTGCCGC GCGGCTGGGA GATCAAAACT 
GACCCGCAGG GGAAGTCATT TTTTGTAGAC CACAACAGTC GTGCCACAAC CTTCATCGAT 
CCCAGAATCC CCTTGCAAAA CGGCAGATTA CCAAACCACC TGACGCACCG CCAGCACCTG 
CAGAGATTAC GCAGCTACAG CGCAGGAGAG GCATCTGAGG TGTCGCGCAC CCGAGGGGTG 
TCTTTGTTAG CCAGGCCTGG AAACAGCCTG GTAGCAGCCA TACGAAACCA AAGCCAGCAG 
GAGCCTGTGC CATTAGCGTA CAATGATAAA ATTGTAGCAT TTTTACGGCA ACCAAACATC 
TTCGAGGTGC TACAGGAGCG TCAGCCCAGC TTTGCCAGGA ATCACTCACT CAAGGATAAA 
GTGCATCACA TCCGAACCGA AGGCACACAG AGACTGGAGA AGCTGTCATG TGATGCTGAC 
CTGGTCATGC TGTTAAGTTT GTTTGAGGAG GACATCATGT CTTACGTTCC TCTCGCATCA 
TTTCACCCCG GGTTCAACTT TTCACCTCGC TGCTCTCCTG GTTCTTCTCC CCAGAACTCT 
CCAGGGACCC AGAGGGCTCG GGCTCCAGCG CCGTACAGAA GAGACTTTGA GGCCAAATTG 
CGCAACTTCT ACAGGAAGCT TGAGGCAAAG GGCTACGGGC AAGGGCCTGG GAAGATAAAA 
TTGATCGTCA GAAGAGATCA TCTTTTAGAA GGAACGTTTA ACCAGGTCAT GGCCTACTCA 
CGTAAAGAGC TGCAGAGAAA CAAGCTGTAC ATTACCTTTG TGGGAGAGGA AGGTCTGGAC 
TACAGTGGCC CATCGCGAGA GTTCTTCTTC CTGCTGTCTC AGGAGCTGTT TAACCCTTAC 
TATGGCCTGT TTGAATACTC CGCCAACGAC ACATACACAG TCCAGATCAG CCCCATGTCT 
GCTTTTGTGG AGAACCACTT GGAATGGTTC CGCTTCAGTG GTCGAATTCT CGGCCTGGCT 
TTGATTCATC AGTATTTGTT GGACGCTTTC TTCACTCGGC CATTTTATAA AGCTCTTCTC 
AGACTACCCA CTGATCTCAG CGATCTGGAG TATCTGGATG AGGAGTTTCA CCAGAGCTTG 
CAGTGGATGA AGGAAAATGA CATCACTGAT GTGCTGGACC TCACCTTCAC TGTCAATGAG 
GAAGTGTTCG GACAGGTGAC AGAGCGGGAG CTGAAGTCTG GCGGGACGAA TGTGCAGGTG 
ACGGAGAAAA ATAAGAAGGA GTACATTGAG CGGATGGTGA AGTGGAGGGT GGAGCGAGGC 
GTTGTCCAGC AGACACAGGC TCTGGTGCGA GGATTTTATG AGGTGGTGGA CTCTCGTCTG 
GTGTCTGTGT TTGATGCCAG AGAGCTGGAG CTGGTCATCG CAGGAACAGC AGAGATCGAT 
TTGAACGACT GGAGAAACAA CACAGAGTAC AGAGGAGGTT ATCATGACGG GCACATTGTG 
ATCCGCTGGT TCTGGGGAGC AGTGGAGCGC TTCAATAATG AGCAGCGGCT GCGTCTGCTG 
CAGTTTGTGA CGGGCACCTC CAGCGTGCCG TATGAGGGCT TTACCGCCCT ACGTGGCAGC 
AATGGACTGA GACGCTTCTG CATTGAGAAA TGGGGAAAGA TCACATCACT CCCAAGGGCG 
CACACATGCT TTAACCGTCT GGATCTTCCA CCATATCCAT CCTACACGAT GTTATACGAG 
AAACTCCTGA TCGCTGTGGA AGAGACCAGC ACTTTTGGCC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297140.1
CDS	147..4754
Translation	MNEEVMIHRQVILTQNLYQSRFLGLAAMASPSRASQGRRRCKDPIRHSFNPEQFPNVDFQNGVAEDLAAAVLRSTSDTDLLTSHCRSTLTVSTSSYTIGQTQDITLSWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHKGQIIWKIEASSYFVEPETKICFKYYHGVSGALRATTPCVTVKNPSAPVFKPVATDDVPQSQGRRRLISFSLSDFQATGLKKGMFFNPDPYLKIAIHPGKHSIFPALPHHGQEKRSGIVCNTINPVWQRERFNFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLEKHAIGDRVVSYTLGRRLPTEHVSGQLQFRFEITSSIHPDDEDMSLSTEQPVAAAEAQAQPQAEDTMSLDLGAPELPLDGDTASVEMPMDVESGQSCAAEADVIEEECLEEEQQEGEAAAAAAAMEQSETNAEETTQVLDPINGAEEEEAENNCAFAEESSDARAGEEDEAISDPQPASEEPDADLEEDCSLRIRTTPRRKPRPCSLPVSELETVIASACGEPETPRSHYIRIHHLLHSLPATQPQTGDSAGPEPDRPMGVEEEDEEEEEEGVTLEDHALTPNGPRRTLPRSRSHERLSDLIQMLDGDGHPLGAEGQTGNQRSPDLSPVACCSHMSPRASGPIRAQSLDSARRSESTVFSSQDDEDEMEQPNGILGSETETEQGAQCREPGDGSCQWEEAPDSHVQSPHGIVGNGEVAGPSGRRECPLPCNHPAVSQLPALRPDPHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQRPTSAATPDGLRRSGSVQQMEQLNRRYQTIQRTMATERREEESGSPRNDRTANTETDSPLQTNELRRDTSCSPGHCQKITLLLQSPAVKFITHPEFFTVLHANYGAYRMFTNSSCLKHMVLKIRRDARNFERYQHNRDLVTFLNRFADAQLELPRGWEIKTDPQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRTRGVSLLARPGNSLVAAIRNQSQQEPVPLAYNDKIVAFLRQPNIFEVLQERQPSFARNHSLKDKVHHIRTEGTQRLEKLSCDADLVMLLSLFEEDIMSYVPLASFHPGFNFSPRCSPGSSPQNSPGTQRARAPAPYRRDFEAKLRNFYRKLEAKGYGQGPGKIKLIVRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPTDLSDLEYLDEEFHQSLQWMKENDITDVLDLTFTVNEEVFGQVTERELKSGGTNVQVTEKNKKEYIERMVKWRVERGVVQQTQALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWGAVERFNNEQRLRLLQFVTGTSSVPYEGFTALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYTMLYEKLLIAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_009298865.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1b (hecw1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298865.2

ORF Insert Sequence:

ATGAACGAGG AGGTGATGAT TCACCGCCAG GTGATACTGA CCCAGAACTT GTACCAGAGC 
AGGTTTCTCG GGCTTGCAGC TATGGCCTCT CCATCTCGTG CATCTCAGGG CAGGAGGCGA 
TGTAAGGACC CGATCCGTCA CAGTTTTAAC CCTGAGCAGT TCCCTAATGT GGACTTCCAG 
AACGGCGTGG CGGAGGATCT GGCGGCGGCC GTGCTGCGCT CTACCAGCGA CACTGACCTG 
CTGACATCCC ACTGCCGCTC CACCCTCACC GTTAGCACCT CCAGCTACAC CATCGGTCAG 
ACGCAGGACA TCACCCTCAG CTGGGACATC AAGGAGGAGG TGGACGCCGG AGACTGGATT 
GGCATGTACC TCATTGATGA GGTTCTGTCA GAGAATTTCC TGGACTACAA AAACCGGGGT 
GTGAACGGCT CGCACAAAGG GCAGATTATC TGGAAGATTG AGGCCAGCTC CTATTTTGTG 
GAACCGGAGA CTAAGATCTG TTTTAAATAT TATCATGGAG TTAGTGGAGC GCTTCGGGCC 
ACAACACCAT GCGTGACTGT GAAGAACCCC TCTGCACCGG TGTTTAAGCC GGTGGCCACA 
GACGATGTTC CTCAGAGTCA GGGAAGAAGG AGACTCATCA GCTTCTCTCT GTCAGATTTC 
CAGGCCACCG GACTGAAAAA AGGCATGTTT TTTAACCCCG ACCCCTACCT AAAGATCGCT 
ATCCACCCTG GCAAACACAG CATCTTCCCA GCGCTGCCTC ACCATGGCCA GGAGAAGAGA 
TCCGGCATCG TCTGCAATAC CATTAATCCT GTTTGGCAGA GGGAGCGCTT TAATTTTGTG 
TCTCTGCCTA CGGATGTGTT GGAGATTGAG GTGAAGGACA AGTTCGCCAA AAGCCGTCCC 
ATCATAAAGC GTTTCTTGGG CAAGCTGTCC ATGCCAGTGC AAAGACTACT GGAGAAGCAT 
GCTATCGGTG ATCGAGTGGT CAGCTATACT CTGGGTCGAA GACTTCCAAC AGAGCATGTC 
AGCGGGCAGC TGCAGTTCAG GTTCGAGATC ACCTCCTCCA TTCATCCAGA TGATGAAGAC 
ATGTCTCTCA GCACAGAGCA GCCGGTGGCA GCAGCAGAAG CTCAAGCTCA GCCTCAAGCT 
GAAGACACCA TGAGTTTAGA TCTCGGAGCC CCGGAGCTGC CCTTAGATGG AGATACGGCT 
TCAGTCGAGA TGCCCATGGA TGTTGAATCT GGTCAGAGTT GTGCAGCTGA AGCAGATGTC 
ATAGAGGAAG AGTGTTTAGA GGAAGAACAG CAGGAAGGAG AAGCAGCAGC AGCAGCAGCA 
GCAATGGAGC AAAGTGAAAC CAATGCAGAA GAAACAACAC AAGTCTTGGA TCCCATCAAT 
GGCGCTGAAG AAGAAGAAGC AGAGAATAAT TGCGCCTTTG CAGAAGAGTC TTCAGATGCT 
CGCGCAGGAG AGGAAGATGA AGCGATTTCA GATCCGCAGC CAGCTTCAGA AGAGCCGGAT 
GCGGATTTAG AGGAAGACTG TTCACTGAGG ATCCGAACCA CTCCAAGAAG GAAACCCCGT 
CCCTGCTCTT TACCTGTCTC TGAGCTTGAG ACGGTCATTG CTTCAGCCTG TGGGGAACCT 
GAAACGCCAC GCTCTCATTA CATCCGAATA CACCACCTGC TCCACAGCCT GCCGGCCACT 
CAACCGCAGA CAGGAGACTC CGCCGGGCCT GAACCAGACC GACCAATGGG TGTAGAGGAG 
GAAGATGAAG AGGAAGAAGA AGAAGGTGTA ACTCTTGAAG ATCATGCTTT AACACCCAAT 
GGCCCTCGCA GGACTCTTCC TCGCAGCCGC TCTCACGAAC GCCTCTCTGA CCTCATTCAG 
ATGCTGGATG GAGATGGACA TCCGCTTGGG GCTGAGGGGC AAACCGGAAA TCAGAGGTCA 
CCTGATTTGA GCCCAGTGGC GTGCTGCAGT CACATGTCCC CGAGAGCTTC TGGTCCCATT 
CGGGCACAAT CCCTAGACAG TGCGCGGAGG TCAGAGAGTA CGGTGTTCTC CTCGCAGGAT 
GACGAGGACG AGATGGAGCA GCCAAATGGC ATTCTGGGAT CAGAGACGGA AACAGAGCAG 
GGCGCTCAAT GTCGGGAGCC AGGCGACGGG AGCTGTCAGT GGGAGGAGGC ACCGGACAGT 
CACGTTCAGA GCCCTCATGG CATTGTGGGT AATGGAGAAG TGGCCGGACC GAGCGGCAGA 
CGAGAATGTC CTTTGCCTTG TAACCACCCT GCAGTGAGTC AGCTGCCTGC CCTGCGGCCC 
GATCCACACC ACTATCCCAC CATTGATGAG CCGCTGCCGC CCAACTGGGA GGCCCGGATC 
GACAGTCACG GTCGTGTGTT TTATGTGGAT CACATTAACC GGACCACCAC TTGGCAAAGA 
CCCACATCTG CAGCCACGCC GGACGGCCTA CGCAGGTCTG GATCCGTCCA ACAGATGGAG 
CAGCTCAACA GGAGGTATCA GACAATCCAG AGGACGATGG CCACCGAGAG GAGAGAAGAG 
GAATCAGGAA GCCCCCGTAA TGACAGAACA GCCAATACTG AGACAGACTC ACCTCTGCAA 
ACAAACGAGT TGAGGAGAGA CACCTCATGT TCTCCAGGCC ACTGTCAGAA GATTACGTTA 
CTGTTACAGA GTCCAGCGGT CAAATTCATC ACTCACCCAG AGTTCTTCAC TGTTCTCCAC 
GCTAATTATG GTGCATATCG AATGTTCACC AACAGCTCGT GTCTGAAACA CATGGTCCTG 
AAAATTCGAC GAGATGCTCG AAATTTTGAG CGCTACCAGC ACAATCGTGA CCTGGTGACG 
TTTCTGAACA GGTTTGCAGA CGCTCAGCTG GAGCTGCCGC GCGGCTGGGA GATCAAAACT 
GACCCGCAGG GGAAGTCATT TTTTGTAGAC CACAACAGTC GTGCCACAAC CTTCATCGAT 
CCCAGAATCC CCTTGCAAAA CGGCAGATTA CCAAACCACC TGACGCACCG CCAGCACCTG 
CAGAGATTAC GCAGCTACAG CGCAGGAGAG GCATCTGAGG TGTCGCGCAC CCGAGGGGTG 
TCTTTGTTAG CCAGGCCTGG AAACAGCCTG GTAGCAGCCA TACGAAACCA AAGCCAGCAG 
GAGCCTGTGC CATTAGCGTA CAATGATAAA ATTGTAGCAT TTTTACGGCA ACCAAACATC 
TTCGAGGTGC TACAGGAGCG TCAGCCCAGC TTTGCCAGGA ATCACTCACT CAAGGATAAA 
GTGCATCACA TCCGAACCGA AGGCACACAG AGACTGGAGA AGCTGTCATG TGATGCTGAC 
CTGGTCATGC TGTTAAGTTT GTTTGAGGAG GACATCATGT CTTACGTTCC TCTCGCATCA 
TTTCACCCCG GGTTCAACTT TTCACCTCGC TGCTCTCCTG GTTCTTCTCC CCAGAACTCT 
CCAGGGACCC AGAGGGCTCG GGCTCCAGCG CCGTACAGAA GAGACTTTGA GGCCAAATTG 
CGCAACTTCT ACAGGAAGCT TGAGGCAAAG GGCTACGGGC AAGGGCCTGG GAAGATAAAA 
TTGATCGTCA GAAGAGATCA TCTTTTAGAA GGAACGTTTA ACCAGGTCAT GGCCTACTCA 
CGTAAAGAGC TGCAGAGAAA CAAGCTGTAC ATTACCTTTG TGGGAGAGGA AGGTCTGGAC 
TACAGTGGCC CATCGCGAGA GTTCTTCTTC CTGCTGTCTC AGGAGCTGTT TAACCCTTAC 
TATGGCCTGT TTGAATACTC CGCCAACGAC ACATACACAG TCCAGATCAG CCCCATGTCT 
GCTTTTGTGG AGAACCACTT GGAATGGTTC CGCTTCAGTG GTCGAATTCT CGGCCTGGCT 
TTGATTCATC AGTATTTGTT GGACGCTTTC TTCACTCGGC CATTTTATAA AGCTCTTCTC 
AGACTACCCA CTGATCTCAG CGATCTGGAG TATCTGGATG AGGAGTTTCA CCAGAGCTTG 
CAGTGGATGA AGGAAAATGA CATCACTGAT GTGCTGGACC TCACCTTCAC TGTCAATGAG 
GAAGTGTTCG GACAGGTGAC AGAGCGGGAG CTGAAGTCTG GCGGGACGAA TGTGCAGGTG 
ACGGAGAAAA ATAAGAAGGA GTACATTGAG CGGATGGTGA AGTGGAGGGT GGAGCGAGGC 
GTTGTCCAGC AGACACAGGC TCTGGTGCGA GGATTTTATG AGGTGGTGGA CTCTCGTCTG 
GTGTCTGTGT TTGATGCCAG AGAGCTGGAG CTGGTCATCG CAGGAACAGC AGAGATCGAT 
TTGAACGACT GGAGAAACAA CACAGAGTAC AGAGGAGGTT ATCATGACGG GCACATTGTG 
ATCCGCTGGT TCTGGGGAGC AGTGGAGCGC TTCAATAATG AGCAGCGGCT GCGTCTGCTG 
CAGTTTGTGA CGGGCACCTC CAGCGTGCCG TATGAGGGCT TTACCGCCCT ACGTGGCAGC 
AATGGACTGA GACGCTTCTG CATTGAGAAA TGGGGAAAGA TCACATCACT CCCAAGGGCG 
CACACATGCT TTAACCGTCT GGATCTTCCA CCATATCCAT CCTACACGAT GTTATACGAG 
AAACTCCTGA TCGCTGTGGA AGAGACCAGC ACTTTTGGCC TTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Sex differences in DNA methylation and expression in zebrafish brain: a test of an extended 'male sex drive' hypothesis.
Gene590(2)307-16(2016 Sep)
Chatterjee A,Lagisz M,Rodger EJ,Zhen L,Stockwell PA,Duncan EJ,Horsfield JA,Jeyakani J,Mathavan S,Ozaki Y,Nakagawa S

Characterization of ?? T Cells from Zebrafish Provides Insights into Their Important Role in Adaptive Humoral Immunity.
Frontiers in immunology7675(2016)
Wan F,Hu CB,Ma JX,Gao K,Xiang LX,Shao JZ

Itch is required for lateral line development in zebrafish.
PloS one9(11)e111799(2014)
Angers A,Drapeau P

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hecw1b cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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