NCU04548 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU04548
Entrez Gene ID	3872087
Full Name	DNA polymerase epsilon
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_950846.3	XP_955939.3	DNA polymerase epsilon

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pole	NP_001121995.1
Arabidopsis thaliana (thale cress)	TIL2	NP_180280.2
Arabidopsis thaliana (thale cress)	TIL1	NP_172303.5
Oryza sativa (rice)	Os02g0511900	NP_001046939.2
Caenorhabditis elegans (roundworm)	F33H2.5	NP_493616.1
Canis lupus familiaris (dog)	POLE	XP_543348.3
Mus musculus (house mouse)	Pole	NP_035262.2
Kluyveromyces lactis	KLLA0C02585g	XP_452312.1
Neurospora crassa	NCU04548	XP_955939.2
Xenopus tropicalis (tropical clawed frog)	pole	XP_004910692.1
Drosophila melanogaster (fruit fly)	DNApol-epsilon	NP_524462.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004615	XP_315205.4
Saccharomyces cerevisiae (baker's yeast)	POL2	NP_014137.1
Eremothecium gossypii	AGOS_AFR657C	NP_986205.2
Magnaporthe oryzae (rice blast fungus)	MGG_03850	XP_003720041.1
Homo sapiens (human)	POLE	NP_006222.2
Pan troglodytes (chimpanzee)	POLE	XP_522564.4
Bos taurus (cattle)	POLE	NP_001178358.1
Rattus norvegicus (Norway rat)	Pole	NP_001100622.2
Gallus gallus (chicken)	POLE	XP_004934482.1
Schizosaccharomyces pombe (fission yeast)	cdc20	NP_596354.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU04548 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU04548 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf09869	XM_950846.3 Latest version!	Neurospora crassa OR74A DNA polymerase epsilon (NCU04548), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU04548 ( XM_950846.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_955939.3 Neurospora crassa OR74A DNA polymerase epsilon (NCU04548), partial mRNA.

Price & Availability↑

CloneID	ONf09869
Clone ID Related Accession (Same CDS sequence)	XM_950846.3
Accession Version	XM_950846.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	DNA polymerase epsilon
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507). On Feb 23, 2015 this sequence version replaced XM_950846.2. COMPLETENESS: incomplete on the 3' end.

ATGCCCAACA CTTCTCTAAG GCGACAGCCT CAAAAGGGCT ACCGTCGCGG TGGCAAGAAC 
GCCTACCATG GCGGATCCAA AACACGAACG TTCGCTGCCT CGGCCAGCGC CCGCGGCGAG 
GCTACCTCGA CAGACGAAAA ATGGGAAAGG ACAGCCCTTG CGAACCAGAT CGACGAGAAC 
ATGGGCTTTG CCCGATACGA TGCCGGGAAG AAGCGCGAAG GCTGGCTGGT CAACGTACAA 
CCGACATCCA TCGACGACCC CCGTATCCCC GGCGGCGGCG GTCGCGCGGC ACTCGACTGC 
TACTTCATCG AAGAGGACGG GTCAACATTC AAAGCCACAG TCGAATATGA GCCATACTTT 
CTGGTCGCAT GTCGAAAAGG TCACGAAGGC GAGGTCGAGG AGTGGTGCAA GAGAGTGCCT 
GGTGGCGGCT GCGTCAAGTC CATCCGGCGG GTCGAGAAGG AAGATCTCAA GATGCCCAAC 
CACTTGCTGG GCTATCGTCG AACATTCTTG GAGCTGAGGT TTCATAACGT GCAGGACTTG 
ATGTCCGCCA AGCGCGATGT CATGCCCATT GCGGAAAAGA ACAAAAAGGG CATGGATGCC 
ATGGATACAT ATGCCGAAGT AGCATCAGCG AACGGCGGGT TTGATCTTTT TGATGACGAT 
TCACGAAATG ACGAGCGACG ACATAATGCT GGTTTTGCAG AGGCGAGCGA TTTCATTGTT 
GATATCCGGG AATACGACGT CCCATACCAT GTGAGAGTAA TGATAGACTT GGACATACGA 
GTTGCTAGAT GGTATCATGT CGAGGCCAAG AATGGAGTCA CGACAGTAAA AGTAAACGAG 
GAACGTTTGG CCATGGCCGA TCCCGTCGTA ATGGCTTTCG ATATCGAGAC CACCAAGTTG 
CCCCTCAAGT TTCCCGACGC CGCTATTGAC CAGATCATGA TGGTCTCATA CATGATCGAT 
GGCCAGGGTT TCTTGATCAC AAATAGAGAG ATTATCTCCG AAGACATTGC TGATTTCGAC 
TACACACCAA AACCCGAGTA CCAAGGTCCG TTTATGATCT TCAACGAACC TGACGAAAAG 
GCCCTTCTCG AAAGATTCTT CCTGCACATC AAGGAGGCCC GACCAACCGT CATCGCCACC 
TACAACGGTG ACTTCTTCGA TTGGCCTTTC GTGGAGGCTC GTGCCAGTAT CAACGGCATC 
GACATGTACC ACGAGATCGG CTGGAAGAAG GACAGTGAGG ATCAGTACAA GTGCAACTAC 
AGTGTGCACA TGGACTGCTT TCACTGGGTA AACCGTGACA GTTATCTCCC CCAGGGTAGT 
AGAGGTCTCA AAGCCGTCAC CGTTGCCAAG CTTGGATACG ATCCCGATGA ACTCGATCCT 
GAGCTTATGA CACCATATGC CAGCGAGAGG CCGCAGACGC TGGCCGAATA CTCCGTCTCC 
GATGCCGTCG CAACATACTA TCTGTACATG AAATATGTGC ATCCTTTCAT CTTCTCTCTC 
TGTACCATCT TGCCGCTAGG CCCAGACGAT ACACTAAGAA AGGGAACCGG TACGCTATGC 
GAGATGTTAT TAATGGTACA GGCTTACCAA AAGGAGATTG TGTTACCCAA TAAACATGTC 
CAGCCTAAGG AGTCCTTCTG GGAAGGACAC TTGTTGGACT CCGAAACGTA CGTTGGTGGT 
CACGTCGAAA GTATCGAAGC TGGCGTCTTC CGCGCCGATA TTCCCGTCAA CTTCGCGGTT 
GACCCTACCG CCGTGGATGA GCTGATTCGG GATCTTGATG CAGCCCTAAC GTTCAGTATT 
ACGGTTGAAG AGAAGAAGGA GTTGAGTGAT ATCGCCAACT ACGACGAGGT CAAGGAGCAG 
ATAACGGCCA GGTTGATGAA CCTCAAAGAG ACGCCCAATC GACTGGAACG GCCGCTCATT 
TACCATCTTG ATGTCGCCTC CATGTACCCC AACATTATGA CAACCAACCG ATTACAACCC 
GATTCCATGA TCTCCGAGTC AACTTGTGCA GCATGCGACT TCAACCGCCC AGGAAAGACT 
TGTGACAGAA GACTACCGTG GGCCTGGAGA GGAGAGTACC TGCCAGCCAA GCGCGACGAG 
TACAACATGA TTCGCCATGC ACTCGAGAAC GAGAGGTTTC CAGGAAAATA CCCAAACAGC 
CCGATGCGGT CATTCCAAGA CCTGAGCGCA GACGAACAAG CCACTCTCAT CCGAAAGAGA 
CTGCAGCTCT ACTCACAAAA GGTGTACCAC AAGATCCACG ATTCCACCAC GATTGTCAGA 
GAAGCGATCA TTTGCCAGCG CGAGAACCCC TTCTACATCA ACACAGTCCG TGACTTCCGT 
GACCGACGAT ACGACTACAA GGGCAAGGCG AAGGTGTGGA AGGGCAAGAC GGATTCGCTC 
AAGAGCTCGG GCGCATCCGC GTCCGAAATC GATAATGCCA AAAAGATGAT TATCTTGTTC 
GATTCCCTGC AGTTGGCCCA CAAGGTCATT CTCAACAGTT TCTACGGATA CGTCATGAGA 
AAGGGATCTA GATGGTACTC GATGGAAATG GCCGGTGTCA CCTGTTTGAC GGGCGCAACC 
ATTATCCAGT TGGCGAGACA GCTCATTGAG CGACTGGGTC GTCCACTCGA GCTAGACACT 
GATGGTATCT GGTGCATGTT GCCGGCGACA TTCCCAGAGA ACTTTGTGTT CAAACTCAAA 
AATGGCAAGA AAATGACCAT CTCGTACCCA TGTGTCATGT TGAACCATCT GGTCCATGAC 
AAGTTCACCA ACCACCAGTA CCAGACTCTG GTCGATCCAA AGACCTTCAA ATACGAGACG 
CACAGCGACA ACTCCATCTT CTTCGAAGTC GACGGGCCGT ACAAGGCCAT GATCCTACCC 
ACCTCCAAAG AAGAAGACAA GAACTTGAAG AAGAGATATG CCGTCTTCAA TGACGATGGC 
AGTCTTGCGG AGCTCAAGGG CTTCGAAGTG AAGCGAAGAG GCGAGTTGAA GCTCATCAAG 
ATTTTCCAGC AGCAGATCTT CAAGTTTTTC CTGGAGGGTA CCACCCTGGC GGAATGCTAT 
AATGCTGTCG CCAAGGTCGC TAACCGCTGG CTTGATGTAC TGCATAGCAA GGGTGCAACG 
CTTGCGGATG AAGAACTTAT GGAACTGATT TCTGAGAATC GCAGCATGAC CAAGACGTTG 
GAGGAGTACG GAAGCCAAAA GTCAACATCC ATCACCACAG CCAAGCGCCT TGCCGACTTT 
TTGGGCGAGC AAATGGTCAA AGACAAGGGT TTGAACTGCA AGTTTATCAT TTGCGCCCGA 
CCCAAGAATG CCCCGGTCAC CGAACGTGCC GTGCCGGTTG CCATCTTCTC TGCCGAAGAA 
GAAACGAAGA GGATGTATCT CAAGAAATGG CTCAAAGAGG AGCCTGCTGA TACCGACCCT 
CGAGCACTCC TTGACTGGGA TTACTATCTC GAACGTCTTG GGTCCGTCAT CCAGAAGCTC 
ATCACCATCC CAGCCGCTCT GCAAAAAGTT CGCAACCCGG TACCGCGCGT GCCGCATCCG 
GACTGGCTGC AGCGGAGGAT CAACATCAAA GACGATAAGA TGAAGCAAAA GAAGCTTACG 
GACTTGTTTG GGCCTACCAC CAAAGGCCCT CTGCAAGATA TCACCCACCT TGCGAACGGC 
ACCCAGCTTG GAGATATGGA GGACTTTGGG AACTTGCTGA AGCCCAAGAC TGTCAGTGAT 
GCTATTTCCG CCACGCAAAA AACGGTGACG GCTCAGAAAC GCAAGGATCC GGAACCAGCA 
GCAGCCGAGA ACCTGGACCC GTTTGCGGCG CTTCCCAAGA AGATGCCGTC GCCTAGCGAG 
GATTACGCGG CTTTCTTGGA GTACCAGAAG CAAAAGTGGA AGCTCCAGAA GCAGGCAAGG 
GCTCGTAGGC GACACTTGTT TGGCGAAAGG CGTGGCAACG CGCAGAGCAA CATCCAGCAG 
ACGTTCCGCA GTCAGGCTGA GATGACATTC CGAAACACTT GGCAGTTGCT GCAGCTGAGG 
GCATCAGACA GTCCAGGCAT TGTCGTTGCC CATGTGCTGA TCGATGCCAA GATCCACACT 
CTCAAGATTA ACGTGCCCCG GCAAGTATTC TTGAACTTGA AGAGTAGGGA ACTGCCCGAC 
ATTGAAGTTG AAGGCTGCCA TGTTGAACAG GTCAACCATA CATTGCCTAA TGGCCACTCT 
TCTGTGCATC TCTTCAAGCT TACGGTGCCG GAGGACATCT ACTTTGCGGA AGCAGAAAAG 
TTCTCTCTGT TATTCAACCA TCCCAGTGTG GAAGGCGTGT ATGAGAAACA GCTACCGCTC 
AACATGCGCG CCCTTCTTCA GCTGGGAAAT CTTTGCACTA TCGACGAAAG CCAACCTGGT 
GTACTTGGAA AGGGTCTTGA CCAAGGATTC GACCTGTCCG GGTTGAAGAG ACCACTCAAG 
CCGAGAGAGT ACTTGGGCGA TGCCCGTATG GCCTACATCT ATCTCTCCCA CATTAGCGCT 
GGTGAGCGCC AGATCTTTGG TCTTTTCTCC ACCACCAGTG ACCAAGCCCA CGTCATCATC 
CAGCAGAGGA CCAAGGATGG AGGGCAAGAC CTGCCAAACA TCAACAAGCT CTATGCCGAC 
ATGTTGGCAA GGAGGAATCA GGAGGCCGCT GGAGCGGAGT GGCAAGAGTG TTTCCCGTAC 
CAGGAAAAGC TGAATATCAA GATCACCCAA GTTACCACCC GCCGGAAAGC TTTCCTTGAG 
GTTGGCGACA TTGTCAAGAA ACTGAGAAAG GAAGAGTCCG GACCGACCAT GGTGGTCATC 
CAATCCTCAC AGCGCAATCT ATTGGTTCAT GAGATTCCCA TCTTGGGGGA ATTCCCTGTG 
ATGCCATTGC GATATGATCA AGGGGACAGC TCACTGCCCC CACTAGGCTG GCAAAGTGTG 
GTGGCCCGTC GGTTGGTCAA TCACTACCTC AGCCTGGGTT CCTGGATCAC CCATATGACG 
GCTCTTGCTC GGTACGGCGA CGTACCGCTA TGCAACCTGG AGCGAGACGA TCCCCGTTTC 
CTGATCGATA TCGCATATGC TCGCCGGCTC ATGGCCAACA ATGTCGTGCT CTGGTGGTCA 
CCGAGTCCGC GCCCCGACCA CGCTGGTTAT GAGAAGGACG ATGTGGTTGG CCCACTCGAT 
CAGGTTCAGA TGCCGTCCGT CAACAATCCC GGCACTTATG CTTCGGTGTG TATAGACCTT 
GAGGTGCGGA ACTTGGCTAT CAACACCATT CTCACATCTT CGCTCATCAA TGAGCTGGAA 
GGTGCGGATA ACATATCTTT CAATCCCGCC GCCGATGCAA CCGGTAGTGG AGACGGCGAA 
GAATTTCTTT ATTCGGAGAA CGCTTTTGCC AATGCTGGCG TGCAGGTGCT CCGTGAGATG 
GTCAAATCAT GGTGGGCGGA AGCTTGCAAG GGGTCCACGA TGGCCGACGT GATGGTTCAG 
CACTTGGTCC GTTGGGTCGA ATCGCCAGCC TCGTGCCTGT ACGATCGTGC TCTGCATTAC 
TATGTCCAGA TGATGAGCCG CAAGGCGCTC CTCCAGCTCA TGGCCGATTT CCGTCGCGTA 
GGATCGCAGG TCATCTTTGC CAGCTCCAAC AGACTTCTCC TCCAGACCAC CAAGTCCGAG 
GTTGGAAATG CGTATGCATA TACCCAGTAC ATTCTCAAGA GCATCAAGGG CAAACCACTC 
TTTCACTTTA TCGACCTCGA AATCAAGGAG TACTGGGACT ACCTGGTATG GTATGACGAG 
TTCAACTACG GCGGCAAGGC GACACAAGAG GTGCTCGAGC GGGACGAACA GGACCTGCAG 
ATGATTATGC ACTGGCAAAT GGCAACCTTT CTCCCTGTCC GTCTGCAGCC CATCTTCCGC 
GACTGGGTCG TCGAGTTTAT CCAGCTGATG CACAGCATCA AGCGACCCTC CTTTGGCAGC 
GACCCAACAG CCACGCCACG TCTCACCCAA CTGCCCGTCA ACATGCGCAA TGGCAATCTC 
GGCGAGGACG GCGCCGTCGG GCAAATCATC CTGGGCAAAG CGTTCGAGAA GCCTCTCAAG 
AAGGAAATCG CCAACCTCAT CAACACGCAA AGGCGCGAGC TCCTGCACCC GGAGCTGGCA 
GGCGATTACA CATTTCCCCA ACTACCGGGA TCTCACCTGT CTCACCTGTC TATGTCCAAT 
GCCAAGTCGA GTAATACCAA GAACGCGGCA GGAAAGAAAG TAGCAGCGTC GGCCAATCCG 
GTGCTTGAGC TGGTCAAGGC CCTGATGCAG GTTTTATCTT TGGACAAGAA CATCACGCTC 
GAAGCGCGGC TGCTGCGCAA GGAACTCCTC GCCATGTTCG ACGTACGCGA GTTCAGCAAG 
GACGGCGCGT TCCAAAACCC ATCGGAAAGT CTGAAACTGG CCCAGTTCAG CTGCGAAAAC 
TGTACCATGG CTAGGGACAT TGATCTCTGT CGAGACGAGG ACTTTATGAG TTTGGCGGCC 
GTTTCTGACG TGTCAGCAGC AGGCGGAGCG GCCGGCGGCG AGTTGGAAGG AGCTGCTCCA 
GGACAAGAAA AGGGAAGGTG GAAATGCCCT TACTGCGAAA TGGACTACGA CAGGAACGAG 
ATTGAGGAGA GGCTGGTGGC GGAGGTGGAG GCCATGGTGG TGGAGTGGAC GACGCAGGAC 
CTCAAGTGCG GCAAGTGCGG GGCGCTGAGG GTGAATGAGT TCATGGAGCA TTACGAGCTG 
CTGTGCTTCA TGATCGATGA GTGGACTGGT GTGGCGCCGG CCGAGGGTGT AAATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_955939.3
CDS	261..7040
Translation	MPNTSLRRQPQKGYRRGGKNAYHGGSKTRTFAASASARGEATSTDEKWERTALANQIDENMGFARYDAGKKREGWLVNVQPTSIDDPRIPGGGGRAALDCYFIEEDGSTFKATVEYEPYFLVACRKGHEGEVEEWCKRVPGGGCVKSIRRVEKEDLKMPNHLLGYRRTFLELRFHNVQDLMSAKRDVMPIAEKNKKGMDAMDTYAEVASANGGFDLFDDDSRNDERRHNAGFAEASDFIVDIREYDVPYHVRVMIDLDIRVARWYHVEAKNGVTTVKVNEERLAMADPVVMAFDIETTKLPLKFPDAAIDQIMMVSYMIDGQGFLITNREIISEDIADFDYTPKPEYQGPFMIFNEPDEKALLERFFLHIKEARPTVIATYNGDFFDWPFVEARASINGIDMYHEIGWKKDSEDQYKCNYSVHMDCFHWVNRDSYLPQGSRGLKAVTVAKLGYDPDELDPELMTPYASERPQTLAEYSVSDAVATYYLYMKYVHPFIFSLCTILPLGPDDTLRKGTGTLCEMLLMVQAYQKEIVLPNKHVQPKESFWEGHLLDSETYVGGHVESIEAGVFRADIPVNFAVDPTAVDELIRDLDAALTFSITVEEKKELSDIANYDEVKEQITARLMNLKETPNRLERPLIYHLDVASMYPNIMTTNRLQPDSMISESTCAACDFNRPGKTCDRRLPWAWRGEYLPAKRDEYNMIRHALENERFPGKYPNSPMRSFQDLSADEQATLIRKRLQLYSQKVYHKIHDSTTIVREAIICQRENPFYINTVRDFRDRRYDYKGKAKVWKGKTDSLKSSGASASEIDNAKKMIILFDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGVTCLTGATIIQLARQLIERLGRPLELDTDGIWCMLPATFPENFVFKLKNGKKMTISYPCVMLNHLVHDKFTNHQYQTLVDPKTFKYETHSDNSIFFEVDGPYKAMILPTSKEEDKNLKKRYAVFNDDGSLAELKGFEVKRRGELKLIKIFQQQIFKFFLEGTTLAECYNAVAKVANRWLDVLHSKGATLADEELMELISENRSMTKTLEEYGSQKSTSITTAKRLADFLGEQMVKDKGLNCKFIICARPKNAPVTERAVPVAIFSAEEETKRMYLKKWLKEEPADTDPRALLDWDYYLERLGSVIQKLITIPAALQKVRNPVPRVPHPDWLQRRINIKDDKMKQKKLTDLFGPTTKGPLQDITHLANGTQLGDMEDFGNLLKPKTVSDAISATQKTVTAQKRKDPEPAAAENLDPFAALPKKMPSPSEDYAAFLEYQKQKWKLQKQARARRRHLFGERRGNAQSNIQQTFRSQAEMTFRNTWQLLQLRASDSPGIVVAHVLIDAKIHTLKINVPRQVFLNLKSRELPDIEVEGCHVEQVNHTLPNGHSSVHLFKLTVPEDIYFAEAEKFSLLFNHPSVEGVYEKQLPLNMRALLQLGNLCTIDESQPGVLGKGLDQGFDLSGLKRPLKPREYLGDARMAYIYLSHISAGERQIFGLFSTTSDQAHVIIQQRTKDGGQDLPNINKLYADMLARRNQEAAGAEWQECFPYQEKLNIKITQVTTRRKAFLEVGDIVKKLRKEESGPTMVVIQSSQRNLLVHEIPILGEFPVMPLRYDQGDSSLPPLGWQSVVARRLVNHYLSLGSWITHMTALARYGDVPLCNLERDDPRFLIDIAYARRLMANNVVLWWSPSPRPDHAGYEKDDVVGPLDQVQMPSVNNPGTYASVCIDLEVRNLAINTILTSSLINELEGADNISFNPAADATGSGDGEEFLYSENAFANAGVQVLREMVKSWWAEACKGSTMADVMVQHLVRWVESPASCLYDRALHYYVQMMSRKALLQLMADFRRVGSQVIFASSNRLLLQTTKSEVGNAYAYTQYILKSIKGKPLFHFIDLEIKEYWDYLVWYDEFNYGGKATQEVLERDEQDLQMIMHWQMATFLPVRLQPIFRDWVVEFIQLMHSIKRPSFGSDPTATPRLTQLPVNMRNGNLGEDGAVGQIILGKAFEKPLKKEIANLINTQRRELLHPELAGDYTFPQLPGSHLSHLSMSNAKSSNTKNAAGKKVAASANPVLELVKALMQVLSLDKNITLEARLLRKELLAMFDVREFSKDGAFQNPSESLKLAQFSCENCTMARDIDLCRDEDFMSLAAVSDVSAAGGAAGGELEGAAPGQEKGRWKCPYCEMDYDRNEIEERLVAEVEAMVVEWTTQDLKCGKCGALRVNEFMEHYELLCFMIDEWTGVAPAEGVNV

Target ORF information:

RefSeq Version	XM_950846.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A DNA polymerase epsilon (NCU04548), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_950846.3

ORF Insert Sequence:

ATGCCCAACA CTTCTCTAAG GCGACAGCCT CAAAAGGGCT ACCGTCGCGG TGGCAAGAAC 
GCCTACCATG GCGGATCCAA AACACGAACG TTCGCTGCCT CGGCCAGCGC CCGCGGCGAG 
GCTACCTCGA CAGACGAAAA ATGGGAAAGG ACAGCCCTTG CGAACCAGAT CGACGAGAAC 
ATGGGCTTTG CCCGATACGA TGCCGGGAAG AAGCGCGAAG GCTGGCTGGT CAACGTACAA 
CCGACATCCA TCGACGACCC CCGTATCCCC GGCGGCGGCG GTCGCGCGGC ACTCGACTGC 
TACTTCATCG AAGAGGACGG GTCAACATTC AAAGCCACAG TCGAATATGA GCCATACTTT 
CTGGTCGCAT GTCGAAAAGG TCACGAAGGC GAGGTCGAGG AGTGGTGCAA GAGAGTGCCT 
GGTGGCGGCT GCGTCAAGTC CATCCGGCGG GTCGAGAAGG AAGATCTCAA GATGCCCAAC 
CACTTGCTGG GCTATCGTCG AACATTCTTG GAGCTGAGGT TTCATAACGT GCAGGACTTG 
ATGTCCGCCA AGCGCGATGT CATGCCCATT GCGGAAAAGA ACAAAAAGGG CATGGATGCC 
ATGGATACAT ATGCCGAAGT AGCATCAGCG AACGGCGGGT TTGATCTTTT TGATGACGAT 
TCACGAAATG ACGAGCGACG ACATAATGCT GGTTTTGCAG AGGCGAGCGA TTTCATTGTT 
GATATCCGGG AATACGACGT CCCATACCAT GTGAGAGTAA TGATAGACTT GGACATACGA 
GTTGCTAGAT GGTATCATGT CGAGGCCAAG AATGGAGTCA CGACAGTAAA AGTAAACGAG 
GAACGTTTGG CCATGGCCGA TCCCGTCGTA ATGGCTTTCG ATATCGAGAC CACCAAGTTG 
CCCCTCAAGT TTCCCGACGC CGCTATTGAC CAGATCATGA TGGTCTCATA CATGATCGAT 
GGCCAGGGTT TCTTGATCAC AAATAGAGAG ATTATCTCCG AAGACATTGC TGATTTCGAC 
TACACACCAA AACCCGAGTA CCAAGGTCCG TTTATGATCT TCAACGAACC TGACGAAAAG 
GCCCTTCTCG AAAGATTCTT CCTGCACATC AAGGAGGCCC GACCAACCGT CATCGCCACC 
TACAACGGTG ACTTCTTCGA TTGGCCTTTC GTGGAGGCTC GTGCCAGTAT CAACGGCATC 
GACATGTACC ACGAGATCGG CTGGAAGAAG GACAGTGAGG ATCAGTACAA GTGCAACTAC 
AGTGTGCACA TGGACTGCTT TCACTGGGTA AACCGTGACA GTTATCTCCC CCAGGGTAGT 
AGAGGTCTCA AAGCCGTCAC CGTTGCCAAG CTTGGATACG ATCCCGATGA ACTCGATCCT 
GAGCTTATGA CACCATATGC CAGCGAGAGG CCGCAGACGC TGGCCGAATA CTCCGTCTCC 
GATGCCGTCG CAACATACTA TCTGTACATG AAATATGTGC ATCCTTTCAT CTTCTCTCTC 
TGTACCATCT TGCCGCTAGG CCCAGACGAT ACACTAAGAA AGGGAACCGG TACGCTATGC 
GAGATGTTAT TAATGGTACA GGCTTACCAA AAGGAGATTG TGTTACCCAA TAAACATGTC 
CAGCCTAAGG AGTCCTTCTG GGAAGGACAC TTGTTGGACT CCGAAACGTA CGTTGGTGGT 
CACGTCGAAA GTATCGAAGC TGGCGTCTTC CGCGCCGATA TTCCCGTCAA CTTCGCGGTT 
GACCCTACCG CCGTGGATGA GCTGATTCGG GATCTTGATG CAGCCCTAAC GTTCAGTATT 
ACGGTTGAAG AGAAGAAGGA GTTGAGTGAT ATCGCCAACT ACGACGAGGT CAAGGAGCAG 
ATAACGGCCA GGTTGATGAA CCTCAAAGAG ACGCCCAATC GACTGGAACG GCCGCTCATT 
TACCATCTTG ATGTCGCCTC CATGTACCCC AACATTATGA CAACCAACCG ATTACAACCC 
GATTCCATGA TCTCCGAGTC AACTTGTGCA GCATGCGACT TCAACCGCCC AGGAAAGACT 
TGTGACAGAA GACTACCGTG GGCCTGGAGA GGAGAGTACC TGCCAGCCAA GCGCGACGAG 
TACAACATGA TTCGCCATGC ACTCGAGAAC GAGAGGTTTC CAGGAAAATA CCCAAACAGC 
CCGATGCGGT CATTCCAAGA CCTGAGCGCA GACGAACAAG CCACTCTCAT CCGAAAGAGA 
CTGCAGCTCT ACTCACAAAA GGTGTACCAC AAGATCCACG ATTCCACCAC GATTGTCAGA 
GAAGCGATCA TTTGCCAGCG CGAGAACCCC TTCTACATCA ACACAGTCCG TGACTTCCGT 
GACCGACGAT ACGACTACAA GGGCAAGGCG AAGGTGTGGA AGGGCAAGAC GGATTCGCTC 
AAGAGCTCGG GCGCATCCGC GTCCGAAATC GATAATGCCA AAAAGATGAT TATCTTGTTC 
GATTCCCTGC AGTTGGCCCA CAAGGTCATT CTCAACAGTT TCTACGGATA CGTCATGAGA 
AAGGGATCTA GATGGTACTC GATGGAAATG GCCGGTGTCA CCTGTTTGAC GGGCGCAACC 
ATTATCCAGT TGGCGAGACA GCTCATTGAG CGACTGGGTC GTCCACTCGA GCTAGACACT 
GATGGTATCT GGTGCATGTT GCCGGCGACA TTCCCAGAGA ACTTTGTGTT CAAACTCAAA 
AATGGCAAGA AAATGACCAT CTCGTACCCA TGTGTCATGT TGAACCATCT GGTCCATGAC 
AAGTTCACCA ACCACCAGTA CCAGACTCTG GTCGATCCAA AGACCTTCAA ATACGAGACG 
CACAGCGACA ACTCCATCTT CTTCGAAGTC GACGGGCCGT ACAAGGCCAT GATCCTACCC 
ACCTCCAAAG AAGAAGACAA GAACTTGAAG AAGAGATATG CCGTCTTCAA TGACGATGGC 
AGTCTTGCGG AGCTCAAGGG CTTCGAAGTG AAGCGAAGAG GCGAGTTGAA GCTCATCAAG 
ATTTTCCAGC AGCAGATCTT CAAGTTTTTC CTGGAGGGTA CCACCCTGGC GGAATGCTAT 
AATGCTGTCG CCAAGGTCGC TAACCGCTGG CTTGATGTAC TGCATAGCAA GGGTGCAACG 
CTTGCGGATG AAGAACTTAT GGAACTGATT TCTGAGAATC GCAGCATGAC CAAGACGTTG 
GAGGAGTACG GAAGCCAAAA GTCAACATCC ATCACCACAG CCAAGCGCCT TGCCGACTTT 
TTGGGCGAGC AAATGGTCAA AGACAAGGGT TTGAACTGCA AGTTTATCAT TTGCGCCCGA 
CCCAAGAATG CCCCGGTCAC CGAACGTGCC GTGCCGGTTG CCATCTTCTC TGCCGAAGAA 
GAAACGAAGA GGATGTATCT CAAGAAATGG CTCAAAGAGG AGCCTGCTGA TACCGACCCT 
CGAGCACTCC TTGACTGGGA TTACTATCTC GAACGTCTTG GGTCCGTCAT CCAGAAGCTC 
ATCACCATCC CAGCCGCTCT GCAAAAAGTT CGCAACCCGG TACCGCGCGT GCCGCATCCG 
GACTGGCTGC AGCGGAGGAT CAACATCAAA GACGATAAGA TGAAGCAAAA GAAGCTTACG 
GACTTGTTTG GGCCTACCAC CAAAGGCCCT CTGCAAGATA TCACCCACCT TGCGAACGGC 
ACCCAGCTTG GAGATATGGA GGACTTTGGG AACTTGCTGA AGCCCAAGAC TGTCAGTGAT 
GCTATTTCCG CCACGCAAAA AACGGTGACG GCTCAGAAAC GCAAGGATCC GGAACCAGCA 
GCAGCCGAGA ACCTGGACCC GTTTGCGGCG CTTCCCAAGA AGATGCCGTC GCCTAGCGAG 
GATTACGCGG CTTTCTTGGA GTACCAGAAG CAAAAGTGGA AGCTCCAGAA GCAGGCAAGG 
GCTCGTAGGC GACACTTGTT TGGCGAAAGG CGTGGCAACG CGCAGAGCAA CATCCAGCAG 
ACGTTCCGCA GTCAGGCTGA GATGACATTC CGAAACACTT GGCAGTTGCT GCAGCTGAGG 
GCATCAGACA GTCCAGGCAT TGTCGTTGCC CATGTGCTGA TCGATGCCAA GATCCACACT 
CTCAAGATTA ACGTGCCCCG GCAAGTATTC TTGAACTTGA AGAGTAGGGA ACTGCCCGAC 
ATTGAAGTTG AAGGCTGCCA TGTTGAACAG GTCAACCATA CATTGCCTAA TGGCCACTCT 
TCTGTGCATC TCTTCAAGCT TACGGTGCCG GAGGACATCT ACTTTGCGGA AGCAGAAAAG 
TTCTCTCTGT TATTCAACCA TCCCAGTGTG GAAGGCGTGT ATGAGAAACA GCTACCGCTC 
AACATGCGCG CCCTTCTTCA GCTGGGAAAT CTTTGCACTA TCGACGAAAG CCAACCTGGT 
GTACTTGGAA AGGGTCTTGA CCAAGGATTC GACCTGTCCG GGTTGAAGAG ACCACTCAAG 
CCGAGAGAGT ACTTGGGCGA TGCCCGTATG GCCTACATCT ATCTCTCCCA CATTAGCGCT 
GGTGAGCGCC AGATCTTTGG TCTTTTCTCC ACCACCAGTG ACCAAGCCCA CGTCATCATC 
CAGCAGAGGA CCAAGGATGG AGGGCAAGAC CTGCCAAACA TCAACAAGCT CTATGCCGAC 
ATGTTGGCAA GGAGGAATCA GGAGGCCGCT GGAGCGGAGT GGCAAGAGTG TTTCCCGTAC 
CAGGAAAAGC TGAATATCAA GATCACCCAA GTTACCACCC GCCGGAAAGC TTTCCTTGAG 
GTTGGCGACA TTGTCAAGAA ACTGAGAAAG GAAGAGTCCG GACCGACCAT GGTGGTCATC 
CAATCCTCAC AGCGCAATCT ATTGGTTCAT GAGATTCCCA TCTTGGGGGA ATTCCCTGTG 
ATGCCATTGC GATATGATCA AGGGGACAGC TCACTGCCCC CACTAGGCTG GCAAAGTGTG 
GTGGCCCGTC GGTTGGTCAA TCACTACCTC AGCCTGGGTT CCTGGATCAC CCATATGACG 
GCTCTTGCTC GGTACGGCGA CGTACCGCTA TGCAACCTGG AGCGAGACGA TCCCCGTTTC 
CTGATCGATA TCGCATATGC TCGCCGGCTC ATGGCCAACA ATGTCGTGCT CTGGTGGTCA 
CCGAGTCCGC GCCCCGACCA CGCTGGTTAT GAGAAGGACG ATGTGGTTGG CCCACTCGAT 
CAGGTTCAGA TGCCGTCCGT CAACAATCCC GGCACTTATG CTTCGGTGTG TATAGACCTT 
GAGGTGCGGA ACTTGGCTAT CAACACCATT CTCACATCTT CGCTCATCAA TGAGCTGGAA 
GGTGCGGATA ACATATCTTT CAATCCCGCC GCCGATGCAA CCGGTAGTGG AGACGGCGAA 
GAATTTCTTT ATTCGGAGAA CGCTTTTGCC AATGCTGGCG TGCAGGTGCT CCGTGAGATG 
GTCAAATCAT GGTGGGCGGA AGCTTGCAAG GGGTCCACGA TGGCCGACGT GATGGTTCAG 
CACTTGGTCC GTTGGGTCGA ATCGCCAGCC TCGTGCCTGT ACGATCGTGC TCTGCATTAC 
TATGTCCAGA TGATGAGCCG CAAGGCGCTC CTCCAGCTCA TGGCCGATTT CCGTCGCGTA 
GGATCGCAGG TCATCTTTGC CAGCTCCAAC AGACTTCTCC TCCAGACCAC CAAGTCCGAG 
GTTGGAAATG CGTATGCATA TACCCAGTAC ATTCTCAAGA GCATCAAGGG CAAACCACTC 
TTTCACTTTA TCGACCTCGA AATCAAGGAG TACTGGGACT ACCTGGTATG GTATGACGAG 
TTCAACTACG GCGGCAAGGC GACACAAGAG GTGCTCGAGC GGGACGAACA GGACCTGCAG 
ATGATTATGC ACTGGCAAAT GGCAACCTTT CTCCCTGTCC GTCTGCAGCC CATCTTCCGC 
GACTGGGTCG TCGAGTTTAT CCAGCTGATG CACAGCATCA AGCGACCCTC CTTTGGCAGC 
GACCCAACAG CCACGCCACG TCTCACCCAA CTGCCCGTCA ACATGCGCAA TGGCAATCTC 
GGCGAGGACG GCGCCGTCGG GCAAATCATC CTGGGCAAAG CGTTCGAGAA GCCTCTCAAG 
AAGGAAATCG CCAACCTCAT CAACACGCAA AGGCGCGAGC TCCTGCACCC GGAGCTGGCA 
GGCGATTACA CATTTCCCCA ACTACCGGGA TCTCACCTGT CTCACCTGTC TATGTCCAAT 
GCCAAGTCGA GTAATACCAA GAACGCGGCA GGAAAGAAAG TAGCAGCGTC GGCCAATCCG 
GTGCTTGAGC TGGTCAAGGC CCTGATGCAG GTTTTATCTT TGGACAAGAA CATCACGCTC 
GAAGCGCGGC TGCTGCGCAA GGAACTCCTC GCCATGTTCG ACGTACGCGA GTTCAGCAAG 
GACGGCGCGT TCCAAAACCC ATCGGAAAGT CTGAAACTGG CCCAGTTCAG CTGCGAAAAC 
TGTACCATGG CTAGGGACAT TGATCTCTGT CGAGACGAGG ACTTTATGAG TTTGGCGGCC 
GTTTCTGACG TGTCAGCAGC AGGCGGAGCG GCCGGCGGCG AGTTGGAAGG AGCTGCTCCA 
GGACAAGAAA AGGGAAGGTG GAAATGCCCT TACTGCGAAA TGGACTACGA CAGGAACGAG 
ATTGAGGAGA GGCTGGTGGC GGAGGTGGAG GCCATGGTGG TGGAGTGGAC GACGCAGGAC 
CTCAAGTGCG GCAAGTGCGG GGCGCTGAGG GTGAATGAGT TCATGGAGCA TTACGAGCTG 
CTGTGCTTCA TGATCGATGA GTGGACTGGT GTGGCGCCGG CCGAGGGTGT AAATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU04548 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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