POLE cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLE
Entrez Gene ID	486223
Full Name	DNA polymerase epsilon, catalytic subunit
General protein information	Preferred Names DNA polymerase epsilon, catalytic subunit Names DNA polymerase epsilon catalytic subunit A polymerase (DNA directed), epsilon, catalytic subunit
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022410247.1	XP_022265955.1	DNA polymerase epsilon catalytic subunit A isoform X2
XM_022410249.1	XP_022265957.1	DNA polymerase epsilon catalytic subunit A isoform X5
XM_014108071.1	XP_013963546.1	DNA polymerase epsilon catalytic subunit A isoform X1
XM_022410246.1	XP_022265954.1	DNA polymerase epsilon catalytic subunit A isoform X1
XM_543348.4	XP_543348.3	DNA polymerase epsilon catalytic subunit A isoform X2
XM_022410248.1	XP_022265956.1	DNA polymerase epsilon catalytic subunit A isoform X3
XM_543348.5	XP_543348.3	DNA polymerase epsilon catalytic subunit A isoform X4

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pole	NP_001121995.1
Arabidopsis thaliana (thale cress)	TIL2	NP_180280.2
Arabidopsis thaliana (thale cress)	TIL1	NP_172303.5
Oryza sativa (rice)	Os02g0511900	NP_001046939.2
Caenorhabditis elegans (roundworm)	F33H2.5	NP_493616.1
Canis lupus familiaris (dog)	POLE	XP_543348.3
Mus musculus (house mouse)	Pole	NP_035262.2
Kluyveromyces lactis	KLLA0C02585g	XP_452312.1
Neurospora crassa	NCU04548	XP_955939.2
Xenopus tropicalis (tropical clawed frog)	pole	XP_004910692.1
Drosophila melanogaster (fruit fly)	DNApol-epsilon	NP_524462.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004615	XP_315205.4
Saccharomyces cerevisiae (baker's yeast)	POL2	NP_014137.1
Eremothecium gossypii	AGOS_AFR657C	NP_986205.2
Magnaporthe oryzae (rice blast fungus)	MGG_03850	XP_003720041.1
Homo sapiens (human)	POLE	NP_006222.2
Pan troglodytes (chimpanzee)	POLE	XP_522564.4
Bos taurus (cattle)	POLE	NP_001178358.1
Rattus norvegicus (Norway rat)	Pole	NP_001100622.2
Gallus gallus (chicken)	POLE	XP_004934482.1
Schizosaccharomyces pombe (fission yeast)	cdc20	NP_596354.1

Gene Ontology

The following POLE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe26058	XM_543348.5 Latest version!	Canis lupus familiaris DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe297419	XM_022410246.1 Latest version!	Canis lupus familiaris DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe297420	XM_022410247.1 Latest version!	Canis lupus familiaris DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe297421	XM_022410248.1 Latest version!	Canis lupus familiaris DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe297422	XM_022410249.1 Latest version!	Canis lupus familiaris DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OCe43391	XM_014108071.1 Latest version!	Canis lupus familiaris polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
View Old Accession Versions >>
OCe26058	XM_543348.4	Canis lupus familiaris polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLE ( XM_543348.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_543348.3 Canis lupus familiaris polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.

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CloneID	OCe26058
Clone ID Related Accession (Same CDS sequence)	XM_543348.4 , XM_543348.5
Accession Version	XM_543348.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	DNA polymerase epsilon catalytic subunit A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_543348.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGC GGTTTGGTTT TGAGAGACTC AAGGAGTCTG GGGAGAAGAC GGGCTGGCTT 
ATCAACATGC ACCCTACTGA GATTTTAGAT GAAGACAAAC GCTTAGTCAG CGCAGTAGAT 
TACTACTTTA TTCAAGATGA TGGGAGCAGA TTTAAGGTGG CCTTGCCCTA CGAGCCGTAT 
TTCTACATTG CAGCCAGAAA GGGTTGTGAA CGAGAAGTTT CATCTTTTCT CTCCAAGAAG 
TTTCAGGGTA GAATTGCTAA AATAGAGACT GTCCCCAAAG AGGATCTAGA CTTGCCAAAT 
CACTTGGTGG GTTTGAAGCG AAATTACATT AAGCTGTCCT TCCACACTGT GGAGGATCTT 
GTTAGAGTGA GGAAGGATGT CTCCCCTGCT GTGAAGAAGA ACCGGGAGCA GGGTCATGCT 
GGTGATGCCT ACACAGCTAT GCTGTCCAGC GTTCTGCAAG GGGGCAGTGT GGTTATTGAT 
GAAGAGGAAG CCTCTAAGAA GGTCACTGAC CAGTTGGACA ATATTGTGGA CATGCGAGAG 
TATGATGTGC CCTACCACAT CCGCCTCTCC ATTGACCTGA AGATCCATGT GGCTCATTGG 
TACAACATCA GATACCGAGG AAGTGCCTTT CCTGTGGAAA TCACCCGCCG AGATGACCTT 
GTTGAACGAC CTGACCCTGT GGTTTTGGCA TTTGACATTG AGACAACCAA ACTGCCTCTC 
AAGTTTCCTG ATGCTGAGAC TGACCAGATT ATGATGATTT CCTACATGAT TGATGGTCAG 
GGCTACCTCA TCACCAACAG GGAGATCGTT TCAGAAGATG TCGAAGATTT CGAGTTCACC 
CCCAAGCCAG AGTATGAAGG ACCTTTTTGT GTCTTTAATG AACCTGACGA GGTTCATCTA 
ATCCAGAGAT GGTTTGACTA TGTCCAGGAG ATGAAACCCA CCATCATGGT CACCTACAAT 
GGGGACTTTT TTGACTGGCC GTTTGTGGAG GCCAGAGCAG CAGTCCACGG ACTAAGCATG 
TACCAGGAGA TCGGGTTCCA GAAGGACAGT CAGGGAGAGT ACAAGGCATC CCAGTGCATC 
CACATGGACT GTCTCAGGTG GGTGAAGAGG GACAGTTACC TTCCTGTGGG CAGCCACAAC 
CTCAAGGCAG CTGCCAAAGC CAAGCTAGGC TATGACCCAG TGGAGCTGGA CCCTGAGGAC 
ATGTGCCGAA TGGCCACCGA GCAGCCCCAG ACTCTGGCCA CGTACTCCGT GTCAGATGCA 
GTGGCCACGT ACTACCTGTA CATGAAATAC GTCCACCCCT TTATATTCGC CCTTTGCACC 
ATTATCCCCA TGGAGCCTGA TGAGGTGCTG CGGAAGGGCT CTGGGACTCT CTGTGAGGCC 
TTGCTGATGG TGCAGGCCTT CCACGCCAAC ATCATCTTCC CCAATAAGCA GGAGCAGGAG 
TTCAACAAGC TGACAGGTGA TGGCCACGTG CTGGATGCCG AGACCTATGT GGGTGGCCAC 
GTGGAGGCCC TTGAGTCAGG CGTCTTTCGC AGTGACATCC CCTGCCGGTT CAGGATGAAT 
CCTGCTGCCT TTGACTTCCT GCTGCAGAGG GTGGAGAAGA CCATGCGCCA TGCCATCGAG 
GAAGAGGAGA AGGTGCCCAT GGAGCATGTC ACCAACTTCC AAGAGGTGTG TGATCAGATT 
AAGACCAAGC TCACCTCCCT GAAAGATGTT CCAAACAGAA TAGAGTGTCC CCTTATCTAC 
CACCTGGATG TGGGGGCCAT GTACCCTAAC ATCATCCTGA CGAACCGCCT GCAGCCCTCT 
GCCGTGGTGG ATGAGGCTAC CTGTGCTGCC TGTGACTTCA ATAAGCCTGG GGCCAACTGC 
CAGAGAAAGA TGGCCTGGCA GTGGAGGGGC GAGTTCATGC CAGCCAGTCG CAGTGAGTAC 
CATCGGATCC AGCACCAGCT GGAGTCAGAG AAGTTCCCTC CCTTGACTGC AGAGGGTCCA 
GCCCGGGCCT TTCACGAGCT CTCTCGAGAG GAGCAGGCTA AATATGAGAA GCGGAGGCTG 
TCAGATTACT GCCGGAAGGC ATACAAGAAG ATACACGTCA CCAGGGTGGA GGAGCGCCTC 
ACCACTATCT GCCAGCGGGA GAACTCTTTC TATGTGGACA CAGTGCGCGC CTTCCGAGAC 
AGGCGTTATG AATTCAAAGG TCTCCACAAG GTGTGGAAGA AGAAACTCTC TGCAGCCATG 
GAGGTGGGTG ATGCGGCCGA AGTGAAGCGC TGCAAGAACA TGGAGGTCCT GTATGACTCC 
CTGCAGCTGG CGCACAAGTG CATCCTCAAC TCCTTCTATG GCTACGTCAT GCGCAGAGGG 
GCTCGCTGGT ACTCCATGGA GATGGCAGGC ATTGTCTGCT TCACAGGGGC TAACATCATC 
ACCCAGGCAC GGGAGCTGAT TGAGCAGATC GGGAGACCCT TGGAGCTGGA CACAGACGGA 
ATATGGTGCG TCCTGCCCAA CAGCTTTCCT GAAAACTTTG TCATCAAGAC AACCAGTGTG 
AAAAAGCCCA AGGTGACCAT TTCCTACCCT GGGGCCATGT TGAACATCAT GGTGAAGGAA 
GGCTTCACCA ATGATCAGTA CCAGGAGCTG ACTGAACCAT CCTCACTCAC CTATGTCACC 
CGTTCAGAGA ACAGCATCTT TTTCGAGGTT GACGGACCCT ACCTTGCCAT GATCCTTCCA 
GCCTCCAAGG AAGAAGGCAA GAAACTGAAG AAGAGATATG CTGTCTTCAA CGAGGACGGT 
TCCCTGGCTG AGCTGAAGGG CTTCGAGATC AAACGCCGAG GGGAGCTGCA GCTGATTAAG 
ATCTTCCAGT CCTCAGTGTT CGAGGCTTTC CTCAAGGGCA GCACCTTAGA AGAAGTGTAT 
GGCTCTGTTG CCAAGGTGGC TGACTACTGG CTGGACGTGC TGTACAGTAA GGCAGCTAAC 
ATGCCCGATT CTGAGCTGTT TGAGCTGATC TCTGAGAATC GCTCCATGTC TCGGAAGCTG 
GAAGATTATG GGGAGCAGAA GTCCACGTCT ATCAGCACAG CCAAGCGCCT GGCTGAGTTC 
CTGGGAGATC AGATGGTGAA GGATGCAGGG CTGAGCTGCC GTTACATCAT CTCTCGGAAG 
CCCGAGGGCT CTCCGGTCAC AGAGAGGGCC ATCCCGCTTG CCATTTTCCA GGCAGAGCCC 
ATGGTGAGGA AGCACTTTCT CCGGAAATGG CTTAAGAGTT CTTCCCTCCA AGACTTTGAT 
ATTCGCACGA TTTTGGATTG GGAGTACTAC ATTGAGCGGC TAGGGAGTGC CATACAGAAG 
ATCATCACGA TCCCTGCAGC TTTGCAGCAG GTAAAGAACC CAGTACCACG CGTCAAGCAC 
CCTGACTGGC TGCACAAGAA ACTTTTGGAG AAGAATGATG TCTGCAAGCA GAAGAAGATC 
AGCGAGCTCT TTGTCCTCGA GGGCAGGAGA CAGGTGGGCA TGGCCCAGGT TCCAGAAGGC 
ACCCAAAGCC TGGGTGCTCC TGATATGGAA GATTTTGGCC TCATGAAGCC ATTGCACTTG 
GCCGTTCCTA TTGCCACTAA GAGGAAGCGT GTCCTCTGGG AGAGCCAGGA GGAGTCACAC 
GACCTGGAGC TGACGGTGCC CTGGCAGGAA ATCTTGGGGC AGCCTCCAGC CCTTGGAACC 
ACCCAGGAAG AGTGGCTGGT TTGGCTCCGG TTCCATAAGA AGAAGTGGCA GCTGCAGGCC 
CGGCAGCGCC TGGCTTGCAA GAAGAGGCGG TGTGTGGAGG TGGCAGAGGG CGAACCTCTG 
CCTGGGGCCA TCCGAGAAAG GCCTGCCACT GGGTTGGGGG GCTTCCTGCG AAGGACCGCC 
CGCAGCATCT TGGACCTTCC ATGGCAGATT GTGCAGATCA GTGAAACCAG CCAGGCTGGT 
TTATTCAGGC TGTGGGCTGT CATCAGCAGT GACTTGTACT GCATCAGGCT GAACATCCCC 
CGAGTATTCT ATGTCAACCA GCAGGTGGCT AAAGCTGAGG AGGGGCCTTC CTATCGCAAG 
GTCAGCCGAG TCCTTCCTCG CTCCAACATG GTCTACAATC TCTATGAGTA CTCCGTGCCT 
GAGGACATGT ACCAGGAACA TATCAATGAA ATCAATACTG AGTTATCAGC CCCAGACATT 
GAGGGCGTGT ATGAGACTCA GGTTCCATTA CTATTCCGCG CCCTAGTGCA GCTGGGCTGT 
GTCTGTGTGG TCAATAAACA GCTGGTGAGG CACCTTTCAG GCTGGGAAGC AGAAACCTTT 
GCTCTCGAGC ACCTGGAGAT GCGTTCTCTG GCTCAGTTCA GCTACCTGGA ACCAGGGAGC 
ATCCGCCACC TCTACCTGTA CCATCATGCA CAGGGCCACA AAGCACTCTT CGGCTTGTTC 
GTGCCCTCCC AGCGCAGAGC ATCTGTGTTT GTGTTGGACA CTGTGCGAAG CAACCAGATG 
CCCAGCCTCA GCGCTCTTTA CTCAGCCGAG CACAGCCTTC TGATGGAGAA AGTGGGCCCG 
GAGCTCCTGC CTCCTCCCAA ACACACTTTT GAAGTTCGGG CGGAAACTGA CATGAAAACC 
ATCTGCAGAG CCATCCAGCG CTTCCTGCTG GCCTACAAAG AGGAGCGCCG TGGGCCCACA 
CTCATCGCTG TTCAGTCCAA CTGGGAGCTA AAGAGGCTGG CTGGTGAGGT TCCTGTCCTA 
GAGGAATTCC CACTAGTGCC TGTCCACGTG GCAGATAAGA TCAGCTATGG AGTCCTGGAC 
TGGCAGCGCC TCGGAGCCCG GCGCATGATC CGCCACTACC TCAACCTGGA CACCTCTCTG 
TCACAGGCCT TCGAGATGAG CAGGTACTTC CACATCCCCA TTGGTAATCT GCCTGAGGAC 
ATCTCCACCT TTGGCTCCGA CCTCTTCTTT GCCCGCCATC TCCAGCGCCA CAGCCACCTG 
CTCTGGCTGT CGCCTACGTC ACGCCCTGAC CTGGGTGGCA AGGAGGCTGA TGACAATCGC 
CTTGTCATGG AATTCGATGA CCAGGCCAGC GTGGAGATCA ATTGTCCAGG CTGCTACTCT 
ACAGTGTGTG TGGAGCTGGA CATCCAAAAC CTGGCTGTCA ACACCATCCT GCAGTCCCAC 
CATATCAATG ACATGGAGGG GGCTGACAGC ATGGGCATCA GCTTTGATGT GATCCAGCAA 
GCCTCTCTGG AGGACATGAT CACTGGCAAC CAGGCCGCCA GCACTCCAGC CAGCTACGAT 
GAGACAGCCC TCTGCTCCAG CACCTTCAGG ATCCTGAAGA GCATGGTGGT GGGCTGGGTG 
AAGGAGATCA CACAGTACCG CAACATCTAT GCTGACAACC AGGTGATGCA CTTTTACCGC 
TGGCTCCGGT CCCCATCATC ACTGCTCCAT GACCCTGTGC TGCATCGCAC ACTCCACAAC 
ATGATGAAGA AGCTCTTTCT GCAGCTCATC GCTGAGTTCA AGCGCTTGGG GTCATCTGTC 
GTCTATGCCA ACTTCAACCG CATCATCCTC TGCACGAAAA AGCGGCGGAT CGAGGATGCC 
ATTGCTTACA TGGAGTACAT CACCAGCAGC ATCCATTCTA AAGAGATCTT CCATTCTTTG 
ACAATATCTT TCTCTCGGTG CTGGGAATTT CTTCTCTGGA TGGATCCCTC TAACTATGGT 
GGAATCAAAG GAAATGCTCC ATCTAGTATC CACTATGGAC AGCAAGACTC CCAAAAAGGG 
GGCAGAGTAG AGGAGGAGGA GGAGGAGGAG GAGGAGGAAG AGGAGGAGGA AGGCAAACAG 
GAACCAGATG TGGAGGACAT GCTGGAAAAC AACTGGAACA TTGTGCAGTT CCTGCCACAG 
GCAGCCTCCT GCCAGAGCTA CTTCCTCATG ATTGTTTCAG CATACATCGT GGCCGTGTAC 
CACAGCATGA AGGAGGAGCT GCGGCGCAGT GCCCCAGGGG GCACACCCCT GAGATGGCGG 
GGGCCCAGCC AGCTCTCCCA GGATGCTGCG GCCTCCAGTG CCCTTCCTGG AATGATCACC 
TTCTCCCAAG ACTACGTGGC CAACGAGCTG ACTCAGAACT TCTTCACCAT CACTCAGAAG 
ATTCAGAAGA AAGTCACAGG CTCCCGGAGC TCCACTGAGC TCTCCAGCAT GTTTCCTGCC 
CTTCCAGGTT CTCACTTGCT GCTCAAAAAC CCCGCATTGG AGTTCATCAA GTACGTGTGC 
AAGGTTCTGT CTCTGGACAC GAACATCACA AACCAGGTGA ACAAATTGAA CCGGGATCTG 
CTTCGTCTGG TGGATGTTGG CGAGTTCTCG GAGGAGGCAC AGTTCCGAGA CCCTTGCCGT 
TCCTACGTGC TCCCTGAGGT CATCTGCCGC AGCTGTAACT TCTGCCGGGA CCTGGACCTG 
TGTAAAGAGT TCGCCTTCTC GCAGGATGGG GCCGTGCTGC CTCAGTGGCT CTGCTCAAAC 
TGTCAGGTTG CCTATGACAC CTCAGTCATC GAGATGGCCC TGGTGGAAGC CCTGCAGAAG 
AAACTGATGG CCTTCACTCT GCAGGACCTG ATCTGCCTGA AGTGCCGAGG TGTGAAGGAG 
ACCAACATGC CTGTGTACTG CAGCTGCGCC GGGGGCTTTA CCCTCACCAT CCACACCAAG 
GTCTTCATGG AGCAGATTGG AATCTTCCAG AACATTGCCC AGCACTATGG CATGTCCTAC 
CTCACAGAGA CCCTGGAGTG GCTGCTGCAG AAGAACCCTC AGCTGGGCCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_543348.3
CDS	64..6777
Translation	MDLRFGFERLKESGEKTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYEPYFYIAARKGCEREVSSFLSKKFQGRIAKIETVPKEDLDLPNHLVGLKRNYIKLSFHTVEDLVRVRKDVSPAVKKNREQGHAGDAYTAMLSSVLQGGSVVIDEEEASKKVTDQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNIRYRGSAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDVEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFDYVQEMKPTIMVTYNGDFFDWPFVEARAAVHGLSMYQEIGFQKDSQGEYKASQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTGDGHVLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPMEHVTNFQEVCDQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAVVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLTAEGPARAFHELSREEQAKYEKRRLSDYCRKAYKKIHVTRVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAMEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRRGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTSVKKPKVTISYPGAMLNIMVKEGFTNDQYQELTEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEIKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPMVRKHFLRKWLKSSSLQDFDIRTILDWEYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVCKQKKISELFVLEGRRQVGMAQVPEGTQSLGAPDMEDFGLMKPLHLAVPIATKRKRVLWESQEESHDLELTVPWQEILGQPPALGTTQEEWLVWLRFHKKKWQLQARQRLACKKRRCVEVAEGEPLPGAIRERPATGLGGFLRRTARSILDLPWQIVQISETSQAGLFRLWAVISSDLYCIRLNIPRVFYVNQQVAKAEEGPSYRKVSRVLPRSNMVYNLYEYSVPEDMYQEHINEINTELSAPDIEGVYETQVPLLFRALVQLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHLYLYHHAQGHKALFGLFVPSQRRASVFVLDTVRSNQMPSLSALYSAEHSLLMEKVGPELLPPPKHTFEVRAETDMKTICRAIQRFLLAYKEERRGPTLIAVQSNWELKRLAGEVPVLEEFPLVPVHVADKISYGVLDWQRLGARRMIRHYLNLDTSLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHSHLLWLSPTSRPDLGGKEADDNRLVMEFDDQASVEINCPGCYSTVCVELDIQNLAVNTILQSHHINDMEGADSMGISFDVIQQASLEDMITGNQAASTPASYDETALCSSTFRILKSMVVGWVKEITQYRNIYADNQVMHFYRWLRSPSSLLHDPVLHRTLHNMMKKLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRIEDAIAYMEYITSSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGNAPSSIHYGQQDSQKGGRVEEEEEEEEEEEEEEGKQEPDVEDMLENNWNIVQFLPQAASCQSYFLMIVSAYIVAVYHSMKEELRRSAPGGTPLRWRGPSQLSQDAAASSALPGMITFSQDYVANELTQNFFTITQKIQKKVTGSRSSTELSSMFPALPGSHLLLKNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCKEFAFSQDGAVLPQWLCSNCQVAYDTSVIEMALVEALQKKLMAFTLQDLICLKCRGVKETNMPVYCSCAGGFTLTIHTKVFMEQIGIFQNIAQHYGMSYLTETLEWLLQKNPQLGH

Target ORF information:

RefSeq Version	XM_543348.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_543348.4

ORF Insert Sequence:

ATGGATTTGC GGTTTGGTTT TGAGAGACTC AAGGAGTCTG GGGAGAAGAC GGGCTGGCTT 
ATCAACATGC ACCCTACTGA GATTTTAGAT GAAGACAAAC GCTTAGTCAG CGCAGTAGAT 
TACTACTTTA TTCAAGATGA TGGGAGCAGA TTTAAGGTGG CCTTGCCCTA CGAGCCGTAT 
TTCTACATTG CAGCCAGAAA GGGTTGTGAA CGAGAAGTTT CATCTTTTCT CTCCAAGAAG 
TTTCAGGGTA GAATTGCTAA AATAGAGACT GTCCCCAAAG AGGATCTAGA CTTGCCAAAT 
CACTTGGTGG GTTTGAAGCG AAATTACATT AAGCTGTCCT TCCACACTGT GGAGGATCTT 
GTTAGAGTGA GGAAGGATGT CTCCCCTGCT GTGAAGAAGA ACCGGGAGCA GGGTCATGCT 
GGTGATGCCT ACACAGCTAT GCTGTCCAGC GTTCTGCAAG GGGGCAGTGT GGTTATTGAT 
GAAGAGGAAG CCTCTAAGAA GGTCACTGAC CAGTTGGACA ATATTGTGGA CATGCGAGAG 
TATGATGTGC CCTACCACAT CCGCCTCTCC ATTGACCTGA AGATCCATGT GGCTCATTGG 
TACAACATCA GATACCGAGG AAGTGCCTTT CCTGTGGAAA TCACCCGCCG AGATGACCTT 
GTTGAACGAC CTGACCCTGT GGTTTTGGCA TTTGACATTG AGACAACCAA ACTGCCTCTC 
AAGTTTCCTG ATGCTGAGAC TGACCAGATT ATGATGATTT CCTACATGAT TGATGGTCAG 
GGCTACCTCA TCACCAACAG GGAGATCGTT TCAGAAGATG TCGAAGATTT CGAGTTCACC 
CCCAAGCCAG AGTATGAAGG ACCTTTTTGT GTCTTTAATG AACCTGACGA GGTTCATCTA 
ATCCAGAGAT GGTTTGACTA TGTCCAGGAG ATGAAACCCA CCATCATGGT CACCTACAAT 
GGGGACTTTT TTGACTGGCC GTTTGTGGAG GCCAGAGCAG CAGTCCACGG ACTAAGCATG 
TACCAGGAGA TCGGGTTCCA GAAGGACAGT CAGGGAGAGT ACAAGGCATC CCAGTGCATC 
CACATGGACT GTCTCAGGTG GGTGAAGAGG GACAGTTACC TTCCTGTGGG CAGCCACAAC 
CTCAAGGCAG CTGCCAAAGC CAAGCTAGGC TATGACCCAG TGGAGCTGGA CCCTGAGGAC 
ATGTGCCGAA TGGCCACCGA GCAGCCCCAG ACTCTGGCCA CGTACTCCGT GTCAGATGCA 
GTGGCCACGT ACTACCTGTA CATGAAATAC GTCCACCCCT TTATATTCGC CCTTTGCACC 
ATTATCCCCA TGGAGCCTGA TGAGGTGCTG CGGAAGGGCT CTGGGACTCT CTGTGAGGCC 
TTGCTGATGG TGCAGGCCTT CCACGCCAAC ATCATCTTCC CCAATAAGCA GGAGCAGGAG 
TTCAACAAGC TGACAGGTGA TGGCCACGTG CTGGATGCCG AGACCTATGT GGGTGGCCAC 
GTGGAGGCCC TTGAGTCAGG CGTCTTTCGC AGTGACATCC CCTGCCGGTT CAGGATGAAT 
CCTGCTGCCT TTGACTTCCT GCTGCAGAGG GTGGAGAAGA CCATGCGCCA TGCCATCGAG 
GAAGAGGAGA AGGTGCCCAT GGAGCATGTC ACCAACTTCC AAGAGGTGTG TGATCAGATT 
AAGACCAAGC TCACCTCCCT GAAAGATGTT CCAAACAGAA TAGAGTGTCC CCTTATCTAC 
CACCTGGATG TGGGGGCCAT GTACCCTAAC ATCATCCTGA CGAACCGCCT GCAGCCCTCT 
GCCGTGGTGG ATGAGGCTAC CTGTGCTGCC TGTGACTTCA ATAAGCCTGG GGCCAACTGC 
CAGAGAAAGA TGGCCTGGCA GTGGAGGGGC GAGTTCATGC CAGCCAGTCG CAGTGAGTAC 
CATCGGATCC AGCACCAGCT GGAGTCAGAG AAGTTCCCTC CCTTGACTGC AGAGGGTCCA 
GCCCGGGCCT TTCACGAGCT CTCTCGAGAG GAGCAGGCTA AATATGAGAA GCGGAGGCTG 
TCAGATTACT GCCGGAAGGC ATACAAGAAG ATACACGTCA CCAGGGTGGA GGAGCGCCTC 
ACCACTATCT GCCAGCGGGA GAACTCTTTC TATGTGGACA CAGTGCGCGC CTTCCGAGAC 
AGGCGTTATG AATTCAAAGG TCTCCACAAG GTGTGGAAGA AGAAACTCTC TGCAGCCATG 
GAGGTGGGTG ATGCGGCCGA AGTGAAGCGC TGCAAGAACA TGGAGGTCCT GTATGACTCC 
CTGCAGCTGG CGCACAAGTG CATCCTCAAC TCCTTCTATG GCTACGTCAT GCGCAGAGGG 
GCTCGCTGGT ACTCCATGGA GATGGCAGGC ATTGTCTGCT TCACAGGGGC TAACATCATC 
ACCCAGGCAC GGGAGCTGAT TGAGCAGATC GGGAGACCCT TGGAGCTGGA CACAGACGGA 
ATATGGTGCG TCCTGCCCAA CAGCTTTCCT GAAAACTTTG TCATCAAGAC AACCAGTGTG 
AAAAAGCCCA AGGTGACCAT TTCCTACCCT GGGGCCATGT TGAACATCAT GGTGAAGGAA 
GGCTTCACCA ATGATCAGTA CCAGGAGCTG ACTGAACCAT CCTCACTCAC CTATGTCACC 
CGTTCAGAGA ACAGCATCTT TTTCGAGGTT GACGGACCCT ACCTTGCCAT GATCCTTCCA 
GCCTCCAAGG AAGAAGGCAA GAAACTGAAG AAGAGATATG CTGTCTTCAA CGAGGACGGT 
TCCCTGGCTG AGCTGAAGGG CTTCGAGATC AAACGCCGAG GGGAGCTGCA GCTGATTAAG 
ATCTTCCAGT CCTCAGTGTT CGAGGCTTTC CTCAAGGGCA GCACCTTAGA AGAAGTGTAT 
GGCTCTGTTG CCAAGGTGGC TGACTACTGG CTGGACGTGC TGTACAGTAA GGCAGCTAAC 
ATGCCCGATT CTGAGCTGTT TGAGCTGATC TCTGAGAATC GCTCCATGTC TCGGAAGCTG 
GAAGATTATG GGGAGCAGAA GTCCACGTCT ATCAGCACAG CCAAGCGCCT GGCTGAGTTC 
CTGGGAGATC AGATGGTGAA GGATGCAGGG CTGAGCTGCC GTTACATCAT CTCTCGGAAG 
CCCGAGGGCT CTCCGGTCAC AGAGAGGGCC ATCCCGCTTG CCATTTTCCA GGCAGAGCCC 
ATGGTGAGGA AGCACTTTCT CCGGAAATGG CTTAAGAGTT CTTCCCTCCA AGACTTTGAT 
ATTCGCACGA TTTTGGATTG GGAGTACTAC ATTGAGCGGC TAGGGAGTGC CATACAGAAG 
ATCATCACGA TCCCTGCAGC TTTGCAGCAG GTAAAGAACC CAGTACCACG CGTCAAGCAC 
CCTGACTGGC TGCACAAGAA ACTTTTGGAG AAGAATGATG TCTGCAAGCA GAAGAAGATC 
AGCGAGCTCT TTGTCCTCGA GGGCAGGAGA CAGGTGGGCA TGGCCCAGGT TCCAGAAGGC 
ACCCAAAGCC TGGGTGCTCC TGATATGGAA GATTTTGGCC TCATGAAGCC ATTGCACTTG 
GCCGTTCCTA TTGCCACTAA GAGGAAGCGT GTCCTCTGGG AGAGCCAGGA GGAGTCACAC 
GACCTGGAGC TGACGGTGCC CTGGCAGGAA ATCTTGGGGC AGCCTCCAGC CCTTGGAACC 
ACCCAGGAAG AGTGGCTGGT TTGGCTCCGG TTCCATAAGA AGAAGTGGCA GCTGCAGGCC 
CGGCAGCGCC TGGCTTGCAA GAAGAGGCGG TGTGTGGAGG TGGCAGAGGG CGAACCTCTG 
CCTGGGGCCA TCCGAGAAAG GCCTGCCACT GGGTTGGGGG GCTTCCTGCG AAGGACCGCC 
CGCAGCATCT TGGACCTTCC ATGGCAGATT GTGCAGATCA GTGAAACCAG CCAGGCTGGT 
TTATTCAGGC TGTGGGCTGT CATCAGCAGT GACTTGTACT GCATCAGGCT GAACATCCCC 
CGAGTATTCT ATGTCAACCA GCAGGTGGCT AAAGCTGAGG AGGGGCCTTC CTATCGCAAG 
GTCAGCCGAG TCCTTCCTCG CTCCAACATG GTCTACAATC TCTATGAGTA CTCCGTGCCT 
GAGGACATGT ACCAGGAACA TATCAATGAA ATCAATACTG AGTTATCAGC CCCAGACATT 
GAGGGCGTGT ATGAGACTCA GGTTCCATTA CTATTCCGCG CCCTAGTGCA GCTGGGCTGT 
GTCTGTGTGG TCAATAAACA GCTGGTGAGG CACCTTTCAG GCTGGGAAGC AGAAACCTTT 
GCTCTCGAGC ACCTGGAGAT GCGTTCTCTG GCTCAGTTCA GCTACCTGGA ACCAGGGAGC 
ATCCGCCACC TCTACCTGTA CCATCATGCA CAGGGCCACA AAGCACTCTT CGGCTTGTTC 
GTGCCCTCCC AGCGCAGAGC ATCTGTGTTT GTGTTGGACA CTGTGCGAAG CAACCAGATG 
CCCAGCCTCA GCGCTCTTTA CTCAGCCGAG CACAGCCTTC TGATGGAGAA AGTGGGCCCG 
GAGCTCCTGC CTCCTCCCAA ACACACTTTT GAAGTTCGGG CGGAAACTGA CATGAAAACC 
ATCTGCAGAG CCATCCAGCG CTTCCTGCTG GCCTACAAAG AGGAGCGCCG TGGGCCCACA 
CTCATCGCTG TTCAGTCCAA CTGGGAGCTA AAGAGGCTGG CTGGTGAGGT TCCTGTCCTA 
GAGGAATTCC CACTAGTGCC TGTCCACGTG GCAGATAAGA TCAGCTATGG AGTCCTGGAC 
TGGCAGCGCC TCGGAGCCCG GCGCATGATC CGCCACTACC TCAACCTGGA CACCTCTCTG 
TCACAGGCCT TCGAGATGAG CAGGTACTTC CACATCCCCA TTGGTAATCT GCCTGAGGAC 
ATCTCCACCT TTGGCTCCGA CCTCTTCTTT GCCCGCCATC TCCAGCGCCA CAGCCACCTG 
CTCTGGCTGT CGCCTACGTC ACGCCCTGAC CTGGGTGGCA AGGAGGCTGA TGACAATCGC 
CTTGTCATGG AATTCGATGA CCAGGCCAGC GTGGAGATCA ATTGTCCAGG CTGCTACTCT 
ACAGTGTGTG TGGAGCTGGA CATCCAAAAC CTGGCTGTCA ACACCATCCT GCAGTCCCAC 
CATATCAATG ACATGGAGGG GGCTGACAGC ATGGGCATCA GCTTTGATGT GATCCAGCAA 
GCCTCTCTGG AGGACATGAT CACTGGCAAC CAGGCCGCCA GCACTCCAGC CAGCTACGAT 
GAGACAGCCC TCTGCTCCAG CACCTTCAGG ATCCTGAAGA GCATGGTGGT GGGCTGGGTG 
AAGGAGATCA CACAGTACCG CAACATCTAT GCTGACAACC AGGTGATGCA CTTTTACCGC 
TGGCTCCGGT CCCCATCATC ACTGCTCCAT GACCCTGTGC TGCATCGCAC ACTCCACAAC 
ATGATGAAGA AGCTCTTTCT GCAGCTCATC GCTGAGTTCA AGCGCTTGGG GTCATCTGTC 
GTCTATGCCA ACTTCAACCG CATCATCCTC TGCACGAAAA AGCGGCGGAT CGAGGATGCC 
ATTGCTTACA TGGAGTACAT CACCAGCAGC ATCCATTCTA AAGAGATCTT CCATTCTTTG 
ACAATATCTT TCTCTCGGTG CTGGGAATTT CTTCTCTGGA TGGATCCCTC TAACTATGGT 
GGAATCAAAG GAAATGCTCC ATCTAGTATC CACTATGGAC AGCAAGACTC CCAAAAAGGG 
GGCAGAGTAG AGGAGGAGGA GGAGGAGGAG GAGGAGGAAG AGGAGGAGGA AGGCAAACAG 
GAACCAGATG TGGAGGACAT GCTGGAAAAC AACTGGAACA TTGTGCAGTT CCTGCCACAG 
GCAGCCTCCT GCCAGAGCTA CTTCCTCATG ATTGTTTCAG CATACATCGT GGCCGTGTAC 
CACAGCATGA AGGAGGAGCT GCGGCGCAGT GCCCCAGGGG GCACACCCCT GAGATGGCGG 
GGGCCCAGCC AGCTCTCCCA GGATGCTGCG GCCTCCAGTG CCCTTCCTGG AATGATCACC 
TTCTCCCAAG ACTACGTGGC CAACGAGCTG ACTCAGAACT TCTTCACCAT CACTCAGAAG 
ATTCAGAAGA AAGTCACAGG CTCCCGGAGC TCCACTGAGC TCTCCAGCAT GTTTCCTGCC 
CTTCCAGGTT CTCACTTGCT GCTCAAAAAC CCCGCATTGG AGTTCATCAA GTACGTGTGC 
AAGGTTCTGT CTCTGGACAC GAACATCACA AACCAGGTGA ACAAATTGAA CCGGGATCTG 
CTTCGTCTGG TGGATGTTGG CGAGTTCTCG GAGGAGGCAC AGTTCCGAGA CCCTTGCCGT 
TCCTACGTGC TCCCTGAGGT CATCTGCCGC AGCTGTAACT TCTGCCGGGA CCTGGACCTG 
TGTAAAGAGT TCGCCTTCTC GCAGGATGGG GCCGTGCTGC CTCAGTGGCT CTGCTCAAAC 
TGTCAGGTTG CCTATGACAC CTCAGTCATC GAGATGGCCC TGGTGGAAGC CCTGCAGAAG 
AAACTGATGG CCTTCACTCT GCAGGACCTG ATCTGCCTGA AGTGCCGAGG TGTGAAGGAG 
ACCAACATGC CTGTGTACTG CAGCTGCGCC GGGGGCTTTA CCCTCACCAT CCACACCAAG 
GTCTTCATGG AGCAGATTGG AATCTTCCAG AACATTGCCC AGCACTATGG CATGTCCTAC 
CTCACAGAGA CCCTGGAGTG GCTGCTGCAG AAGAACCCTC AGCTGGGCCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_543348.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_543348.3 Canis lupus familiaris DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X4, mRNA.

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CloneID	OCe26058
Clone ID Related Accession (Same CDS sequence)	XM_543348.4 , XM_543348.5
Accession Version	XM_543348.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DNA polymerase epsilon catalytic subunit A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_543348.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGC GGTTTGGTTT TGAGAGACTC AAGGAGTCTG GGGAGAAGAC GGGCTGGCTT 
ATCAACATGC ACCCTACTGA GATTTTAGAT GAAGACAAAC GCTTAGTCAG CGCAGTAGAT 
TACTACTTTA TTCAAGATGA TGGGAGCAGA TTTAAGGTGG CCTTGCCCTA CGAGCCGTAT 
TTCTACATTG CAGCCAGAAA GGGTTGTGAA CGAGAAGTTT CATCTTTTCT CTCCAAGAAG 
TTTCAGGGTA GAATTGCTAA AATAGAGACT GTCCCCAAAG AGGATCTAGA CTTGCCAAAT 
CACTTGGTGG GTTTGAAGCG AAATTACATT AAGCTGTCCT TCCACACTGT GGAGGATCTT 
GTTAGAGTGA GGAAGGATGT CTCCCCTGCT GTGAAGAAGA ACCGGGAGCA GGGTCATGCT 
GGTGATGCCT ACACAGCTAT GCTGTCCAGC GTTCTGCAAG GGGGCAGTGT GGTTATTGAT 
GAAGAGGAAG CCTCTAAGAA GGTCACTGAC CAGTTGGACA ATATTGTGGA CATGCGAGAG 
TATGATGTGC CCTACCACAT CCGCCTCTCC ATTGACCTGA AGATCCATGT GGCTCATTGG 
TACAACATCA GATACCGAGG AAGTGCCTTT CCTGTGGAAA TCACCCGCCG AGATGACCTT 
GTTGAACGAC CTGACCCTGT GGTTTTGGCA TTTGACATTG AGACAACCAA ACTGCCTCTC 
AAGTTTCCTG ATGCTGAGAC TGACCAGATT ATGATGATTT CCTACATGAT TGATGGTCAG 
GGCTACCTCA TCACCAACAG GGAGATCGTT TCAGAAGATG TCGAAGATTT CGAGTTCACC 
CCCAAGCCAG AGTATGAAGG ACCTTTTTGT GTCTTTAATG AACCTGACGA GGTTCATCTA 
ATCCAGAGAT GGTTTGACTA TGTCCAGGAG ATGAAACCCA CCATCATGGT CACCTACAAT 
GGGGACTTTT TTGACTGGCC GTTTGTGGAG GCCAGAGCAG CAGTCCACGG ACTAAGCATG 
TACCAGGAGA TCGGGTTCCA GAAGGACAGT CAGGGAGAGT ACAAGGCATC CCAGTGCATC 
CACATGGACT GTCTCAGGTG GGTGAAGAGG GACAGTTACC TTCCTGTGGG CAGCCACAAC 
CTCAAGGCAG CTGCCAAAGC CAAGCTAGGC TATGACCCAG TGGAGCTGGA CCCTGAGGAC 
ATGTGCCGAA TGGCCACCGA GCAGCCCCAG ACTCTGGCCA CGTACTCCGT GTCAGATGCA 
GTGGCCACGT ACTACCTGTA CATGAAATAC GTCCACCCCT TTATATTCGC CCTTTGCACC 
ATTATCCCCA TGGAGCCTGA TGAGGTGCTG CGGAAGGGCT CTGGGACTCT CTGTGAGGCC 
TTGCTGATGG TGCAGGCCTT CCACGCCAAC ATCATCTTCC CCAATAAGCA GGAGCAGGAG 
TTCAACAAGC TGACAGGTGA TGGCCACGTG CTGGATGCCG AGACCTATGT GGGTGGCCAC 
GTGGAGGCCC TTGAGTCAGG CGTCTTTCGC AGTGACATCC CCTGCCGGTT CAGGATGAAT 
CCTGCTGCCT TTGACTTCCT GCTGCAGAGG GTGGAGAAGA CCATGCGCCA TGCCATCGAG 
GAAGAGGAGA AGGTGCCCAT GGAGCATGTC ACCAACTTCC AAGAGGTGTG TGATCAGATT 
AAGACCAAGC TCACCTCCCT GAAAGATGTT CCAAACAGAA TAGAGTGTCC CCTTATCTAC 
CACCTGGATG TGGGGGCCAT GTACCCTAAC ATCATCCTGA CGAACCGCCT GCAGCCCTCT 
GCCGTGGTGG ATGAGGCTAC CTGTGCTGCC TGTGACTTCA ATAAGCCTGG GGCCAACTGC 
CAGAGAAAGA TGGCCTGGCA GTGGAGGGGC GAGTTCATGC CAGCCAGTCG CAGTGAGTAC 
CATCGGATCC AGCACCAGCT GGAGTCAGAG AAGTTCCCTC CCTTGACTGC AGAGGGTCCA 
GCCCGGGCCT TTCACGAGCT CTCTCGAGAG GAGCAGGCTA AATATGAGAA GCGGAGGCTG 
TCAGATTACT GCCGGAAGGC ATACAAGAAG ATACACGTCA CCAGGGTGGA GGAGCGCCTC 
ACCACTATCT GCCAGCGGGA GAACTCTTTC TATGTGGACA CAGTGCGCGC CTTCCGAGAC 
AGGCGTTATG AATTCAAAGG TCTCCACAAG GTGTGGAAGA AGAAACTCTC TGCAGCCATG 
GAGGTGGGTG ATGCGGCCGA AGTGAAGCGC TGCAAGAACA TGGAGGTCCT GTATGACTCC 
CTGCAGCTGG CGCACAAGTG CATCCTCAAC TCCTTCTATG GCTACGTCAT GCGCAGAGGG 
GCTCGCTGGT ACTCCATGGA GATGGCAGGC ATTGTCTGCT TCACAGGGGC TAACATCATC 
ACCCAGGCAC GGGAGCTGAT TGAGCAGATC GGGAGACCCT TGGAGCTGGA CACAGACGGA 
ATATGGTGCG TCCTGCCCAA CAGCTTTCCT GAAAACTTTG TCATCAAGAC AACCAGTGTG 
AAAAAGCCCA AGGTGACCAT TTCCTACCCT GGGGCCATGT TGAACATCAT GGTGAAGGAA 
GGCTTCACCA ATGATCAGTA CCAGGAGCTG ACTGAACCAT CCTCACTCAC CTATGTCACC 
CGTTCAGAGA ACAGCATCTT TTTCGAGGTT GACGGACCCT ACCTTGCCAT GATCCTTCCA 
GCCTCCAAGG AAGAAGGCAA GAAACTGAAG AAGAGATATG CTGTCTTCAA CGAGGACGGT 
TCCCTGGCTG AGCTGAAGGG CTTCGAGATC AAACGCCGAG GGGAGCTGCA GCTGATTAAG 
ATCTTCCAGT CCTCAGTGTT CGAGGCTTTC CTCAAGGGCA GCACCTTAGA AGAAGTGTAT 
GGCTCTGTTG CCAAGGTGGC TGACTACTGG CTGGACGTGC TGTACAGTAA GGCAGCTAAC 
ATGCCCGATT CTGAGCTGTT TGAGCTGATC TCTGAGAATC GCTCCATGTC TCGGAAGCTG 
GAAGATTATG GGGAGCAGAA GTCCACGTCT ATCAGCACAG CCAAGCGCCT GGCTGAGTTC 
CTGGGAGATC AGATGGTGAA GGATGCAGGG CTGAGCTGCC GTTACATCAT CTCTCGGAAG 
CCCGAGGGCT CTCCGGTCAC AGAGAGGGCC ATCCCGCTTG CCATTTTCCA GGCAGAGCCC 
ATGGTGAGGA AGCACTTTCT CCGGAAATGG CTTAAGAGTT CTTCCCTCCA AGACTTTGAT 
ATTCGCACGA TTTTGGATTG GGAGTACTAC ATTGAGCGGC TAGGGAGTGC CATACAGAAG 
ATCATCACGA TCCCTGCAGC TTTGCAGCAG GTAAAGAACC CAGTACCACG CGTCAAGCAC 
CCTGACTGGC TGCACAAGAA ACTTTTGGAG AAGAATGATG TCTGCAAGCA GAAGAAGATC 
AGCGAGCTCT TTGTCCTCGA GGGCAGGAGA CAGGTGGGCA TGGCCCAGGT TCCAGAAGGC 
ACCCAAAGCC TGGGTGCTCC TGATATGGAA GATTTTGGCC TCATGAAGCC ATTGCACTTG 
GCCGTTCCTA TTGCCACTAA GAGGAAGCGT GTCCTCTGGG AGAGCCAGGA GGAGTCACAC 
GACCTGGAGC TGACGGTGCC CTGGCAGGAA ATCTTGGGGC AGCCTCCAGC CCTTGGAACC 
ACCCAGGAAG AGTGGCTGGT TTGGCTCCGG TTCCATAAGA AGAAGTGGCA GCTGCAGGCC 
CGGCAGCGCC TGGCTTGCAA GAAGAGGCGG TGTGTGGAGG TGGCAGAGGG CGAACCTCTG 
CCTGGGGCCA TCCGAGAAAG GCCTGCCACT GGGTTGGGGG GCTTCCTGCG AAGGACCGCC 
CGCAGCATCT TGGACCTTCC ATGGCAGATT GTGCAGATCA GTGAAACCAG CCAGGCTGGT 
TTATTCAGGC TGTGGGCTGT CATCAGCAGT GACTTGTACT GCATCAGGCT GAACATCCCC 
CGAGTATTCT ATGTCAACCA GCAGGTGGCT AAAGCTGAGG AGGGGCCTTC CTATCGCAAG 
GTCAGCCGAG TCCTTCCTCG CTCCAACATG GTCTACAATC TCTATGAGTA CTCCGTGCCT 
GAGGACATGT ACCAGGAACA TATCAATGAA ATCAATACTG AGTTATCAGC CCCAGACATT 
GAGGGCGTGT ATGAGACTCA GGTTCCATTA CTATTCCGCG CCCTAGTGCA GCTGGGCTGT 
GTCTGTGTGG TCAATAAACA GCTGGTGAGG CACCTTTCAG GCTGGGAAGC AGAAACCTTT 
GCTCTCGAGC ACCTGGAGAT GCGTTCTCTG GCTCAGTTCA GCTACCTGGA ACCAGGGAGC 
ATCCGCCACC TCTACCTGTA CCATCATGCA CAGGGCCACA AAGCACTCTT CGGCTTGTTC 
GTGCCCTCCC AGCGCAGAGC ATCTGTGTTT GTGTTGGACA CTGTGCGAAG CAACCAGATG 
CCCAGCCTCA GCGCTCTTTA CTCAGCCGAG CACAGCCTTC TGATGGAGAA AGTGGGCCCG 
GAGCTCCTGC CTCCTCCCAA ACACACTTTT GAAGTTCGGG CGGAAACTGA CATGAAAACC 
ATCTGCAGAG CCATCCAGCG CTTCCTGCTG GCCTACAAAG AGGAGCGCCG TGGGCCCACA 
CTCATCGCTG TTCAGTCCAA CTGGGAGCTA AAGAGGCTGG CTGGTGAGGT TCCTGTCCTA 
GAGGAATTCC CACTAGTGCC TGTCCACGTG GCAGATAAGA TCAGCTATGG AGTCCTGGAC 
TGGCAGCGCC TCGGAGCCCG GCGCATGATC CGCCACTACC TCAACCTGGA CACCTCTCTG 
TCACAGGCCT TCGAGATGAG CAGGTACTTC CACATCCCCA TTGGTAATCT GCCTGAGGAC 
ATCTCCACCT TTGGCTCCGA CCTCTTCTTT GCCCGCCATC TCCAGCGCCA CAGCCACCTG 
CTCTGGCTGT CGCCTACGTC ACGCCCTGAC CTGGGTGGCA AGGAGGCTGA TGACAATCGC 
CTTGTCATGG AATTCGATGA CCAGGCCAGC GTGGAGATCA ATTGTCCAGG CTGCTACTCT 
ACAGTGTGTG TGGAGCTGGA CATCCAAAAC CTGGCTGTCA ACACCATCCT GCAGTCCCAC 
CATATCAATG ACATGGAGGG GGCTGACAGC ATGGGCATCA GCTTTGATGT GATCCAGCAA 
GCCTCTCTGG AGGACATGAT CACTGGCAAC CAGGCCGCCA GCACTCCAGC CAGCTACGAT 
GAGACAGCCC TCTGCTCCAG CACCTTCAGG ATCCTGAAGA GCATGGTGGT GGGCTGGGTG 
AAGGAGATCA CACAGTACCG CAACATCTAT GCTGACAACC AGGTGATGCA CTTTTACCGC 
TGGCTCCGGT CCCCATCATC ACTGCTCCAT GACCCTGTGC TGCATCGCAC ACTCCACAAC 
ATGATGAAGA AGCTCTTTCT GCAGCTCATC GCTGAGTTCA AGCGCTTGGG GTCATCTGTC 
GTCTATGCCA ACTTCAACCG CATCATCCTC TGCACGAAAA AGCGGCGGAT CGAGGATGCC 
ATTGCTTACA TGGAGTACAT CACCAGCAGC ATCCATTCTA AAGAGATCTT CCATTCTTTG 
ACAATATCTT TCTCTCGGTG CTGGGAATTT CTTCTCTGGA TGGATCCCTC TAACTATGGT 
GGAATCAAAG GAAATGCTCC ATCTAGTATC CACTATGGAC AGCAAGACTC CCAAAAAGGG 
GGCAGAGTAG AGGAGGAGGA GGAGGAGGAG GAGGAGGAAG AGGAGGAGGA AGGCAAACAG 
GAACCAGATG TGGAGGACAT GCTGGAAAAC AACTGGAACA TTGTGCAGTT CCTGCCACAG 
GCAGCCTCCT GCCAGAGCTA CTTCCTCATG ATTGTTTCAG CATACATCGT GGCCGTGTAC 
CACAGCATGA AGGAGGAGCT GCGGCGCAGT GCCCCAGGGG GCACACCCCT GAGATGGCGG 
GGGCCCAGCC AGCTCTCCCA GGATGCTGCG GCCTCCAGTG CCCTTCCTGG AATGATCACC 
TTCTCCCAAG ACTACGTGGC CAACGAGCTG ACTCAGAACT TCTTCACCAT CACTCAGAAG 
ATTCAGAAGA AAGTCACAGG CTCCCGGAGC TCCACTGAGC TCTCCAGCAT GTTTCCTGCC 
CTTCCAGGTT CTCACTTGCT GCTCAAAAAC CCCGCATTGG AGTTCATCAA GTACGTGTGC 
AAGGTTCTGT CTCTGGACAC GAACATCACA AACCAGGTGA ACAAATTGAA CCGGGATCTG 
CTTCGTCTGG TGGATGTTGG CGAGTTCTCG GAGGAGGCAC AGTTCCGAGA CCCTTGCCGT 
TCCTACGTGC TCCCTGAGGT CATCTGCCGC AGCTGTAACT TCTGCCGGGA CCTGGACCTG 
TGTAAAGAGT TCGCCTTCTC GCAGGATGGG GCCGTGCTGC CTCAGTGGCT CTGCTCAAAC 
TGTCAGGTTG CCTATGACAC CTCAGTCATC GAGATGGCCC TGGTGGAAGC CCTGCAGAAG 
AAACTGATGG CCTTCACTCT GCAGGACCTG ATCTGCCTGA AGTGCCGAGG TGTGAAGGAG 
ACCAACATGC CTGTGTACTG CAGCTGCGCC GGGGGCTTTA CCCTCACCAT CCACACCAAG 
GTCTTCATGG AGCAGATTGG AATCTTCCAG AACATTGCCC AGCACTATGG CATGTCCTAC 
CTCACAGAGA CCCTGGAGTG GCTGCTGCAG AAGAACCCTC AGCTGGGCCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_543348.3
CDS	85..6798
Translation	MDLRFGFERLKESGEKTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYEPYFYIAARKGCEREVSSFLSKKFQGRIAKIETVPKEDLDLPNHLVGLKRNYIKLSFHTVEDLVRVRKDVSPAVKKNREQGHAGDAYTAMLSSVLQGGSVVIDEEEASKKVTDQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNIRYRGSAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDVEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFDYVQEMKPTIMVTYNGDFFDWPFVEARAAVHGLSMYQEIGFQKDSQGEYKASQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTGDGHVLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPMEHVTNFQEVCDQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAVVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLTAEGPARAFHELSREEQAKYEKRRLSDYCRKAYKKIHVTRVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAMEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRRGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTSVKKPKVTISYPGAMLNIMVKEGFTNDQYQELTEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEIKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPMVRKHFLRKWLKSSSLQDFDIRTILDWEYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVCKQKKISELFVLEGRRQVGMAQVPEGTQSLGAPDMEDFGLMKPLHLAVPIATKRKRVLWESQEESHDLELTVPWQEILGQPPALGTTQEEWLVWLRFHKKKWQLQARQRLACKKRRCVEVAEGEPLPGAIRERPATGLGGFLRRTARSILDLPWQIVQISETSQAGLFRLWAVISSDLYCIRLNIPRVFYVNQQVAKAEEGPSYRKVSRVLPRSNMVYNLYEYSVPEDMYQEHINEINTELSAPDIEGVYETQVPLLFRALVQLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHLYLYHHAQGHKALFGLFVPSQRRASVFVLDTVRSNQMPSLSALYSAEHSLLMEKVGPELLPPPKHTFEVRAETDMKTICRAIQRFLLAYKEERRGPTLIAVQSNWELKRLAGEVPVLEEFPLVPVHVADKISYGVLDWQRLGARRMIRHYLNLDTSLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHSHLLWLSPTSRPDLGGKEADDNRLVMEFDDQASVEINCPGCYSTVCVELDIQNLAVNTILQSHHINDMEGADSMGISFDVIQQASLEDMITGNQAASTPASYDETALCSSTFRILKSMVVGWVKEITQYRNIYADNQVMHFYRWLRSPSSLLHDPVLHRTLHNMMKKLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRIEDAIAYMEYITSSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGNAPSSIHYGQQDSQKGGRVEEEEEEEEEEEEEEGKQEPDVEDMLENNWNIVQFLPQAASCQSYFLMIVSAYIVAVYHSMKEELRRSAPGGTPLRWRGPSQLSQDAAASSALPGMITFSQDYVANELTQNFFTITQKIQKKVTGSRSSTELSSMFPALPGSHLLLKNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCKEFAFSQDGAVLPQWLCSNCQVAYDTSVIEMALVEALQKKLMAFTLQDLICLKCRGVKETNMPVYCSCAGGFTLTIHTKVFMEQIGIFQNIAQHYGMSYLTETLEWLLQKNPQLGH

Target ORF information:

RefSeq Version	XM_543348.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_543348.5

ORF Insert Sequence:

ATGGATTTGC GGTTTGGTTT TGAGAGACTC AAGGAGTCTG GGGAGAAGAC GGGCTGGCTT 
ATCAACATGC ACCCTACTGA GATTTTAGAT GAAGACAAAC GCTTAGTCAG CGCAGTAGAT 
TACTACTTTA TTCAAGATGA TGGGAGCAGA TTTAAGGTGG CCTTGCCCTA CGAGCCGTAT 
TTCTACATTG CAGCCAGAAA GGGTTGTGAA CGAGAAGTTT CATCTTTTCT CTCCAAGAAG 
TTTCAGGGTA GAATTGCTAA AATAGAGACT GTCCCCAAAG AGGATCTAGA CTTGCCAAAT 
CACTTGGTGG GTTTGAAGCG AAATTACATT AAGCTGTCCT TCCACACTGT GGAGGATCTT 
GTTAGAGTGA GGAAGGATGT CTCCCCTGCT GTGAAGAAGA ACCGGGAGCA GGGTCATGCT 
GGTGATGCCT ACACAGCTAT GCTGTCCAGC GTTCTGCAAG GGGGCAGTGT GGTTATTGAT 
GAAGAGGAAG CCTCTAAGAA GGTCACTGAC CAGTTGGACA ATATTGTGGA CATGCGAGAG 
TATGATGTGC CCTACCACAT CCGCCTCTCC ATTGACCTGA AGATCCATGT GGCTCATTGG 
TACAACATCA GATACCGAGG AAGTGCCTTT CCTGTGGAAA TCACCCGCCG AGATGACCTT 
GTTGAACGAC CTGACCCTGT GGTTTTGGCA TTTGACATTG AGACAACCAA ACTGCCTCTC 
AAGTTTCCTG ATGCTGAGAC TGACCAGATT ATGATGATTT CCTACATGAT TGATGGTCAG 
GGCTACCTCA TCACCAACAG GGAGATCGTT TCAGAAGATG TCGAAGATTT CGAGTTCACC 
CCCAAGCCAG AGTATGAAGG ACCTTTTTGT GTCTTTAATG AACCTGACGA GGTTCATCTA 
ATCCAGAGAT GGTTTGACTA TGTCCAGGAG ATGAAACCCA CCATCATGGT CACCTACAAT 
GGGGACTTTT TTGACTGGCC GTTTGTGGAG GCCAGAGCAG CAGTCCACGG ACTAAGCATG 
TACCAGGAGA TCGGGTTCCA GAAGGACAGT CAGGGAGAGT ACAAGGCATC CCAGTGCATC 
CACATGGACT GTCTCAGGTG GGTGAAGAGG GACAGTTACC TTCCTGTGGG CAGCCACAAC 
CTCAAGGCAG CTGCCAAAGC CAAGCTAGGC TATGACCCAG TGGAGCTGGA CCCTGAGGAC 
ATGTGCCGAA TGGCCACCGA GCAGCCCCAG ACTCTGGCCA CGTACTCCGT GTCAGATGCA 
GTGGCCACGT ACTACCTGTA CATGAAATAC GTCCACCCCT TTATATTCGC CCTTTGCACC 
ATTATCCCCA TGGAGCCTGA TGAGGTGCTG CGGAAGGGCT CTGGGACTCT CTGTGAGGCC 
TTGCTGATGG TGCAGGCCTT CCACGCCAAC ATCATCTTCC CCAATAAGCA GGAGCAGGAG 
TTCAACAAGC TGACAGGTGA TGGCCACGTG CTGGATGCCG AGACCTATGT GGGTGGCCAC 
GTGGAGGCCC TTGAGTCAGG CGTCTTTCGC AGTGACATCC CCTGCCGGTT CAGGATGAAT 
CCTGCTGCCT TTGACTTCCT GCTGCAGAGG GTGGAGAAGA CCATGCGCCA TGCCATCGAG 
GAAGAGGAGA AGGTGCCCAT GGAGCATGTC ACCAACTTCC AAGAGGTGTG TGATCAGATT 
AAGACCAAGC TCACCTCCCT GAAAGATGTT CCAAACAGAA TAGAGTGTCC CCTTATCTAC 
CACCTGGATG TGGGGGCCAT GTACCCTAAC ATCATCCTGA CGAACCGCCT GCAGCCCTCT 
GCCGTGGTGG ATGAGGCTAC CTGTGCTGCC TGTGACTTCA ATAAGCCTGG GGCCAACTGC 
CAGAGAAAGA TGGCCTGGCA GTGGAGGGGC GAGTTCATGC CAGCCAGTCG CAGTGAGTAC 
CATCGGATCC AGCACCAGCT GGAGTCAGAG AAGTTCCCTC CCTTGACTGC AGAGGGTCCA 
GCCCGGGCCT TTCACGAGCT CTCTCGAGAG GAGCAGGCTA AATATGAGAA GCGGAGGCTG 
TCAGATTACT GCCGGAAGGC ATACAAGAAG ATACACGTCA CCAGGGTGGA GGAGCGCCTC 
ACCACTATCT GCCAGCGGGA GAACTCTTTC TATGTGGACA CAGTGCGCGC CTTCCGAGAC 
AGGCGTTATG AATTCAAAGG TCTCCACAAG GTGTGGAAGA AGAAACTCTC TGCAGCCATG 
GAGGTGGGTG ATGCGGCCGA AGTGAAGCGC TGCAAGAACA TGGAGGTCCT GTATGACTCC 
CTGCAGCTGG CGCACAAGTG CATCCTCAAC TCCTTCTATG GCTACGTCAT GCGCAGAGGG 
GCTCGCTGGT ACTCCATGGA GATGGCAGGC ATTGTCTGCT TCACAGGGGC TAACATCATC 
ACCCAGGCAC GGGAGCTGAT TGAGCAGATC GGGAGACCCT TGGAGCTGGA CACAGACGGA 
ATATGGTGCG TCCTGCCCAA CAGCTTTCCT GAAAACTTTG TCATCAAGAC AACCAGTGTG 
AAAAAGCCCA AGGTGACCAT TTCCTACCCT GGGGCCATGT TGAACATCAT GGTGAAGGAA 
GGCTTCACCA ATGATCAGTA CCAGGAGCTG ACTGAACCAT CCTCACTCAC CTATGTCACC 
CGTTCAGAGA ACAGCATCTT TTTCGAGGTT GACGGACCCT ACCTTGCCAT GATCCTTCCA 
GCCTCCAAGG AAGAAGGCAA GAAACTGAAG AAGAGATATG CTGTCTTCAA CGAGGACGGT 
TCCCTGGCTG AGCTGAAGGG CTTCGAGATC AAACGCCGAG GGGAGCTGCA GCTGATTAAG 
ATCTTCCAGT CCTCAGTGTT CGAGGCTTTC CTCAAGGGCA GCACCTTAGA AGAAGTGTAT 
GGCTCTGTTG CCAAGGTGGC TGACTACTGG CTGGACGTGC TGTACAGTAA GGCAGCTAAC 
ATGCCCGATT CTGAGCTGTT TGAGCTGATC TCTGAGAATC GCTCCATGTC TCGGAAGCTG 
GAAGATTATG GGGAGCAGAA GTCCACGTCT ATCAGCACAG CCAAGCGCCT GGCTGAGTTC 
CTGGGAGATC AGATGGTGAA GGATGCAGGG CTGAGCTGCC GTTACATCAT CTCTCGGAAG 
CCCGAGGGCT CTCCGGTCAC AGAGAGGGCC ATCCCGCTTG CCATTTTCCA GGCAGAGCCC 
ATGGTGAGGA AGCACTTTCT CCGGAAATGG CTTAAGAGTT CTTCCCTCCA AGACTTTGAT 
ATTCGCACGA TTTTGGATTG GGAGTACTAC ATTGAGCGGC TAGGGAGTGC CATACAGAAG 
ATCATCACGA TCCCTGCAGC TTTGCAGCAG GTAAAGAACC CAGTACCACG CGTCAAGCAC 
CCTGACTGGC TGCACAAGAA ACTTTTGGAG AAGAATGATG TCTGCAAGCA GAAGAAGATC 
AGCGAGCTCT TTGTCCTCGA GGGCAGGAGA CAGGTGGGCA TGGCCCAGGT TCCAGAAGGC 
ACCCAAAGCC TGGGTGCTCC TGATATGGAA GATTTTGGCC TCATGAAGCC ATTGCACTTG 
GCCGTTCCTA TTGCCACTAA GAGGAAGCGT GTCCTCTGGG AGAGCCAGGA GGAGTCACAC 
GACCTGGAGC TGACGGTGCC CTGGCAGGAA ATCTTGGGGC AGCCTCCAGC CCTTGGAACC 
ACCCAGGAAG AGTGGCTGGT TTGGCTCCGG TTCCATAAGA AGAAGTGGCA GCTGCAGGCC 
CGGCAGCGCC TGGCTTGCAA GAAGAGGCGG TGTGTGGAGG TGGCAGAGGG CGAACCTCTG 
CCTGGGGCCA TCCGAGAAAG GCCTGCCACT GGGTTGGGGG GCTTCCTGCG AAGGACCGCC 
CGCAGCATCT TGGACCTTCC ATGGCAGATT GTGCAGATCA GTGAAACCAG CCAGGCTGGT 
TTATTCAGGC TGTGGGCTGT CATCAGCAGT GACTTGTACT GCATCAGGCT GAACATCCCC 
CGAGTATTCT ATGTCAACCA GCAGGTGGCT AAAGCTGAGG AGGGGCCTTC CTATCGCAAG 
GTCAGCCGAG TCCTTCCTCG CTCCAACATG GTCTACAATC TCTATGAGTA CTCCGTGCCT 
GAGGACATGT ACCAGGAACA TATCAATGAA ATCAATACTG AGTTATCAGC CCCAGACATT 
GAGGGCGTGT ATGAGACTCA GGTTCCATTA CTATTCCGCG CCCTAGTGCA GCTGGGCTGT 
GTCTGTGTGG TCAATAAACA GCTGGTGAGG CACCTTTCAG GCTGGGAAGC AGAAACCTTT 
GCTCTCGAGC ACCTGGAGAT GCGTTCTCTG GCTCAGTTCA GCTACCTGGA ACCAGGGAGC 
ATCCGCCACC TCTACCTGTA CCATCATGCA CAGGGCCACA AAGCACTCTT CGGCTTGTTC 
GTGCCCTCCC AGCGCAGAGC ATCTGTGTTT GTGTTGGACA CTGTGCGAAG CAACCAGATG 
CCCAGCCTCA GCGCTCTTTA CTCAGCCGAG CACAGCCTTC TGATGGAGAA AGTGGGCCCG 
GAGCTCCTGC CTCCTCCCAA ACACACTTTT GAAGTTCGGG CGGAAACTGA CATGAAAACC 
ATCTGCAGAG CCATCCAGCG CTTCCTGCTG GCCTACAAAG AGGAGCGCCG TGGGCCCACA 
CTCATCGCTG TTCAGTCCAA CTGGGAGCTA AAGAGGCTGG CTGGTGAGGT TCCTGTCCTA 
GAGGAATTCC CACTAGTGCC TGTCCACGTG GCAGATAAGA TCAGCTATGG AGTCCTGGAC 
TGGCAGCGCC TCGGAGCCCG GCGCATGATC CGCCACTACC TCAACCTGGA CACCTCTCTG 
TCACAGGCCT TCGAGATGAG CAGGTACTTC CACATCCCCA TTGGTAATCT GCCTGAGGAC 
ATCTCCACCT TTGGCTCCGA CCTCTTCTTT GCCCGCCATC TCCAGCGCCA CAGCCACCTG 
CTCTGGCTGT CGCCTACGTC ACGCCCTGAC CTGGGTGGCA AGGAGGCTGA TGACAATCGC 
CTTGTCATGG AATTCGATGA CCAGGCCAGC GTGGAGATCA ATTGTCCAGG CTGCTACTCT 
ACAGTGTGTG TGGAGCTGGA CATCCAAAAC CTGGCTGTCA ACACCATCCT GCAGTCCCAC 
CATATCAATG ACATGGAGGG GGCTGACAGC ATGGGCATCA GCTTTGATGT GATCCAGCAA 
GCCTCTCTGG AGGACATGAT CACTGGCAAC CAGGCCGCCA GCACTCCAGC CAGCTACGAT 
GAGACAGCCC TCTGCTCCAG CACCTTCAGG ATCCTGAAGA GCATGGTGGT GGGCTGGGTG 
AAGGAGATCA CACAGTACCG CAACATCTAT GCTGACAACC AGGTGATGCA CTTTTACCGC 
TGGCTCCGGT CCCCATCATC ACTGCTCCAT GACCCTGTGC TGCATCGCAC ACTCCACAAC 
ATGATGAAGA AGCTCTTTCT GCAGCTCATC GCTGAGTTCA AGCGCTTGGG GTCATCTGTC 
GTCTATGCCA ACTTCAACCG CATCATCCTC TGCACGAAAA AGCGGCGGAT CGAGGATGCC 
ATTGCTTACA TGGAGTACAT CACCAGCAGC ATCCATTCTA AAGAGATCTT CCATTCTTTG 
ACAATATCTT TCTCTCGGTG CTGGGAATTT CTTCTCTGGA TGGATCCCTC TAACTATGGT 
GGAATCAAAG GAAATGCTCC ATCTAGTATC CACTATGGAC AGCAAGACTC CCAAAAAGGG 
GGCAGAGTAG AGGAGGAGGA GGAGGAGGAG GAGGAGGAAG AGGAGGAGGA AGGCAAACAG 
GAACCAGATG TGGAGGACAT GCTGGAAAAC AACTGGAACA TTGTGCAGTT CCTGCCACAG 
GCAGCCTCCT GCCAGAGCTA CTTCCTCATG ATTGTTTCAG CATACATCGT GGCCGTGTAC 
CACAGCATGA AGGAGGAGCT GCGGCGCAGT GCCCCAGGGG GCACACCCCT GAGATGGCGG 
GGGCCCAGCC AGCTCTCCCA GGATGCTGCG GCCTCCAGTG CCCTTCCTGG AATGATCACC 
TTCTCCCAAG ACTACGTGGC CAACGAGCTG ACTCAGAACT TCTTCACCAT CACTCAGAAG 
ATTCAGAAGA AAGTCACAGG CTCCCGGAGC TCCACTGAGC TCTCCAGCAT GTTTCCTGCC 
CTTCCAGGTT CTCACTTGCT GCTCAAAAAC CCCGCATTGG AGTTCATCAA GTACGTGTGC 
AAGGTTCTGT CTCTGGACAC GAACATCACA AACCAGGTGA ACAAATTGAA CCGGGATCTG 
CTTCGTCTGG TGGATGTTGG CGAGTTCTCG GAGGAGGCAC AGTTCCGAGA CCCTTGCCGT 
TCCTACGTGC TCCCTGAGGT CATCTGCCGC AGCTGTAACT TCTGCCGGGA CCTGGACCTG 
TGTAAAGAGT TCGCCTTCTC GCAGGATGGG GCCGTGCTGC CTCAGTGGCT CTGCTCAAAC 
TGTCAGGTTG CCTATGACAC CTCAGTCATC GAGATGGCCC TGGTGGAAGC CCTGCAGAAG 
AAACTGATGG CCTTCACTCT GCAGGACCTG ATCTGCCTGA AGTGCCGAGG TGTGAAGGAG 
ACCAACATGC CTGTGTACTG CAGCTGCGCC GGGGGCTTTA CCCTCACCAT CCACACCAAG 
GTCTTCATGG AGCAGATTGG AATCTTCCAG AACATTGCCC AGCACTATGG CATGTCCTAC 
CTCACAGAGA CCCTGGAGTG GCTGCTGCAG AAGAACCCTC AGCTGGGCCA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_022410246.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265954.1 Canis lupus familiaris DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.

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CloneID	OCe297419
Clone ID Related Accession (Same CDS sequence)	XM_022410246.1
Accession Version	XM_022410246.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DNA polymerase epsilon catalytic subunit A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGGTG AAGACAGAAT TTCCTTTCTC CTTTCATTCA GGGATGATGG CCTCTCCTCT 
TCTGTCTCAG CACTTAAGCG CCTGGAGCGC AGTCAGTGGA CAGATAAGAT GGATTTGCGG 
TTTGGTTTTG AGAGACTCAA GGAGTCTGGG GAGAAGACGG GCTGGCTTAT CAACATGCAC 
CCTACTGAGA TTTTAGATGA AGACAAACGC TTAGTCAGCG CAGTAGATTA CTACTTTATT 
CAAGATGATG GGAGCAGATT TAAGGTGGCC TTGCCCTACG AGCCGTATTT CTACATTGCA 
GCCAGAAAGG GTTGTGAACG AGAAGTTTCA TCTTTTCTCT CCAAGAAGTT TCAGGGTAGA 
ATTGCTAAAA TAGAGACTGT CCCCAAAGAG GATCTAGACT TGCCAAATCA CTTGGTGGGT 
TTGAAGCGAA ATTACATTAA GCTGTCCTTC CACACTGTGG AGGATCTTGT TAGAGTGAGG 
AAGGATGTCT CCCCTGCTGT GAAGAAGAAC CGGGAGCAGG GTCATGCTGG TGATGCCTAC 
ACAGCTATGC TGTCCAGCGT TCTGCAAGGG GGCAGTGTGG TTATTGATGA AGAGGAAGCC 
TCTAAGAAGG TCACTGACCA GTTGGACAAT ATTGTGGACA TGCGAGAGTA TGATGTGCCC 
TACCACATCC GCCTCTCCAT TGACCTGAAG ATCCATGTGG CTCATTGGTA CAACATCAGA 
TACCGAGGAA GTGCCTTTCC TGTGGAAATC ACCCGCCGAG ATGACCTTGT TGAACGACCT 
GACCCTGTGG TTTTGGCATT TGACATTGAG ACAACCAAAC TGCCTCTCAA GTTTCCTGAT 
GCTGAGACTG ACCAGATTAT GATGATTTCC TACATGATTG ATGGTCAGGG CTACCTCATC 
ACCAACAGGG AGATCGTTTC AGAAGATGTC GAAGATTTCG AGTTCACCCC CAAGCCAGAG 
TATGAAGGAC CTTTTTGTGT CTTTAATGAA CCTGACGAGG TTCATCTAAT CCAGAGATGG 
TTTGACTATG TCCAGGAGAT GAAACCCACC ATCATGGTCA CCTACAATGG GGACTTTTTT 
GACTGGCCGT TTGTGGAGGC CAGAGCAGCA GTCCACGGAC TAAGCATGTA CCAGGAGATC 
GGGTTCCAGA AGGACAGTCA GGGAGAGTAC AAGGCATCCC AGTGCATCCA CATGGACTGT 
CTCAGGTGGG TGAAGAGGGA CAGTTACCTT CCTGTGGGCA GCCACAACCT CAAGGCAGCT 
GCCAAAGCCA AGCTAGGCTA TGACCCAGTG GAGCTGGACC CTGAGGACAT GTGCCGAATG 
GCCACCGAGC AGCCCCAGAC TCTGGCCACG TACTCCGTGT CAGATGCAGT GGCCACGTAC 
TACCTGTACA TGAAATACGT CCACCCCTTT ATATTCGCCC TTTGCACCAT TATCCCCATG 
GAGCCTGATG AGGTGCTGCG GAAGGGCTCT GGGACTCTCT GTGAGGCCTT GCTGATGGTG 
CAGGCCTTCC ACGCCAACAT CATCTTCCCC AATAAGCAGG AGCAGGAGTT CAACAAGCTG 
ACAGGTGATG GCCACGTGCT GGATGCCGAG ACCTATGTGG GTGGCCACGT GGAGGCCCTT 
GAGTCAGGCG TCTTTCGCAG TGACATCCCC TGCCGGTTCA GGATGAATCC TGCTGCCTTT 
GACTTCCTGC TGCAGAGGGT GGAGAAGACC ATGCGCCATG CCATCGAGGA AGAGGAGAAG 
GTGCCCATGG AGCATGTCAC CAACTTCCAA GAGGTGTGTG ATCAGATTAA GACCAAGCTC 
ACCTCCCTGA AAGATGTTCC AAACAGAATA GAGTGTCCCC TTATCTACCA CCTGGATGTG 
GGGGCCATGT ACCCTAACAT CATCCTGACG AACCGCCTGC AGCCCTCTGC CGTGGTGGAT 
GAGGCTACCT GTGCTGCCTG TGACTTCAAT AAGCCTGGGG CCAACTGCCA GAGAAAGATG 
GCCTGGCAGT GGAGGGGCGA GTTCATGCCA GCCAGTCGCA GTGAGTACCA TCGGATCCAG 
CACCAGCTGG AGTCAGAGAA GTTCCCTCCC TTGACTGCAG AGGGTCCAGC CCGGGCCTTT 
CACGAGCTCT CTCGAGAGGA GCAGGCTAAA TATGAGAAGC GGAGGCTGTC AGATTACTGC 
CGGAAGGCAT ACAAGAAGAT ACACGTCACC AGGGTGGAGG AGCGCCTCAC CACTATCTGC 
CAGCGGGAGA ACTCTTTCTA TGTGGACACA GTGCGCGCCT TCCGAGACAG GCGTTATGAA 
TTCAAAGGTC TCCACAAGGT GTGGAAGAAG AAACTCTCTG CAGCCATGGA GGTGGGTGAT 
GCGGCCGAAG TGAAGCGCTG CAAGAACATG GAGGTCCTGT ATGACTCCCT GCAGCTGGCG 
CACAAGTGCA TCCTCAACTC CTTCTATGGC TACGTCATGC GCAGAGGGGC TCGCTGGTAC 
TCCATGGAGA TGGCAGGCAT TGTCTGCTTC ACAGGGGCTA ACATCATCAC CCAGGCACGG 
GAGCTGATTG AGCAGATCGG GAGACCCTTG GAGCTGGACA CAGACGGAAT ATGGTGCGTC 
CTGCCCAACA GCTTTCCTGA AAACTTTGTC ATCAAGACAA CCAGTGTGAA AAAGCCCAAG 
GTGACCATTT CCTACCCTGG GGCCATGTTG AACATCATGG TGAAGGAAGG CTTCACCAAT 
GATCAGTACC AGGAGCTGAC TGAACCATCC TCACTCACCT ATGTCACCCG TTCAGAGAAC 
AGCATCTTTT TCGAGGTTGA CGGACCCTAC CTTGCCATGA TCCTTCCAGC CTCCAAGGAA 
GAAGGCAAGA AACTGAAGAA GAGATATGCT GTCTTCAACG AGGACGGTTC CCTGGCTGAG 
CTGAAGGGCT TCGAGATCAA ACGCCGAGGG GAGCTGCAGC TGATTAAGAT CTTCCAGTCC 
TCAGTGTTCG AGGCTTTCCT CAAGGGCAGC ACCTTAGAAG AAGTGTATGG CTCTGTTGCC 
AAGGTGGCTG ACTACTGGCT GGACGTGCTG TACAGTAAGG CAGCTAACAT GCCCGATTCT 
GAGCTGTTTG AGCTGATCTC TGAGAATCGC TCCATGTCTC GGAAGCTGGA AGATTATGGG 
GAGCAGAAGT CCACGTCTAT CAGCACAGCC AAGCGCCTGG CTGAGTTCCT GGGAGATCAG 
ATGGTGAAGG ATGCAGGGCT GAGCTGCCGT TACATCATCT CTCGGAAGCC CGAGGGCTCT 
CCGGTCACAG AGAGGGCCAT CCCGCTTGCC ATTTTCCAGG CAGAGCCCAT GGTGAGGAAG 
CACTTTCTCC GGAAATGGCT TAAGAGTTCT TCCCTCCAAG ACTTTGATAT TCGCACGATT 
TTGGATTGGG AGTACTACAT TGAGCGGCTA GGGAGTGCCA TACAGAAGAT CATCACGATC 
CCTGCAGCTT TGCAGCAGGT AAAGAACCCA GTACCACGCG TCAAGCACCC TGACTGGCTG 
CACAAGAAAC TTTTGGAGAA GAATGATGTC TGCAAGCAGA AGAAGATCAG CGAGCTCTTT 
GTCCTCGAGG GCAGGAGACA GGTGGGCATG GCCCAGGTTC CAGAAGGCAC CCAAAGCCTG 
GGTGCTCCTG ATATGGAAGA TTTTGGCCTC ATGAAGCCAT TGCACTTGGC CGTTCCTATT 
GCCACTAAGA GGAAGCGTGT CCTCTGGGAG AGCCAGGAGG AGTCACACGA CCTGGAGCTG 
ACGGTGCCCT GGCAGGAAAT CTTGGGGCAG CCTCCAGCCC TTGGAACCAC CCAGGAAGAG 
TGGCTGGTTT GGCTCCGGTT CCATAAGAAG AAGTGGCAGC TGCAGGCCCG GCAGCGCCTG 
GCTTGCAAGA AGAGGCGGTG TGTGGAGGTG GCAGAGGGCG AACCTCTGCC TGGGGCCATC 
CGAGAAAGGC CTGCCACTGG GTTGGGGGGC TTCCTGCGAA GGACCGCCCG CAGCATCTTG 
GACCTTCCAT GGCAGATTGT GCAGATCAGT GAAACCAGCC AGGCTGGTTT ATTCAGGCTG 
TGGGCTGTCA TCAGCAGTGA CTTGTACTGC ATCAGGCTGA ACATCCCCCG AGTATTCTAT 
GTCAACCAGC AGGTGGCTAA AGCTGAGGAG GGGCCTTCCT ATCGCAAGGT CAGCCGAGTC 
CTTCCTCGCT CCAACATGGT CTACAATCTC TATGAGTACT CCGTGCCTGA GGACATGTAC 
CAGGAACATA TCAATGAAAT CAATACTGAG TTATCAGCCC CAGACATTGA GGGCGTGTAT 
GAGACTCAGG TTCCATTACT ATTCCGCGCC CTAGTGCAGC TGGGCTGTGT CTGTGTGGTC 
AATAAACAGC TGGTGAGGCA CCTTTCAGGC TGGGAAGCAG AAACCTTTGC TCTCGAGCAC 
CTGGAGATGC GTTCTCTGGC TCAGTTCAGC TACCTGGAAC CAGGGAGCAT CCGCCACCTC 
TACCTGTACC ATCATGCACA GGGCCACAAA GCACTCTTCG GCTTGTTCGT GCCCTCCCAG 
CGCAGAGCAT CTGTGTTTGT GTTGGACACT GTGCGAAGCA ACCAGATGCC CAGCCTCAGC 
GCTCTTTACT CAGCCGAGCA CAGCCTTCTG ATGGAGAAAG TGGGCCCGGA GCTCCTGCCT 
CCTCCCAAAC ACACTTTTGA AGTTCGGGCG GAAACTGACA TGAAAACCAT CTGCAGAGCC 
ATCCAGCGCT TCCTGCTGGC CTACAAAGAG GAGCGCCGTG GGCCCACACT CATCGCTGTT 
CAGTCCAACT GGGAGCTAAA GAGGCTGGCT GGTGAGGTTC CTGTCCTAGA GGAATTCCCA 
CTAGTGCCTG TCCACGTGGC AGATAAGATC AGCTATGGAG TCCTGGACTG GCAGCGCCTC 
GGAGCCCGGC GCATGATCCG CCACTACCTC AACCTGGACA CCTCTCTGTC ACAGGCCTTC 
GAGATGAGCA GGTACTTCCA CATCCCCATT GGTAATCTGC CTGAGGACAT CTCCACCTTT 
GGCTCCGACC TCTTCTTTGC CCGCCATCTC CAGCGCCACA GCCACCTGCT CTGGCTGTCG 
CCTACGTCAC GCCCTGACCT GGGTGGCAAG GAGGCTGATG ACAATCGCCT TGTCATGGAA 
TTCGATGACC AGGCCAGCGT GGAGATCAAT TGTCCAGGCT GCTACTCTAC AGTGTGTGTG 
GAGCTGGACA TCCAAAACCT GGCTGTCAAC ACCATCCTGC AGTCCCACCA TATCAATGAC 
ATGGAGGGGG CTGACAGCAT GGGCATCAGC TTTGATGTGA TCCAGCAAGC CTCTCTGGAG 
GACATGATCA CTGGCAACCA GGCCGCCAGC ACTCCAGCCA GCTACGATGA GACAGCCCTC 
TGCTCCAGCA CCTTCAGGAT CCTGAAGAGC ATGGTGGTGG GCTGGGTGAA GGAGATCACA 
CAGTACCGCA ACATCTATGC TGACAACCAG GTGATGCACT TTTACCGCTG GCTCCGGTCC 
CCATCATCAC TGCTCCATGA CCCTGTGCTG CATCGCACAC TCCACAACAT GATGAAGAAG 
CTCTTTCTGC AGCTCATCGC TGAGTTCAAG CGCTTGGGGT CATCTGTCGT CTATGCCAAC 
TTCAACCGCA TCATCCTCTG CACGAAAAAG CGGCGGATCG AGGATGCCAT TGCTTACATG 
GAGTACATCA CCAGCAGCAT CCATTCTAAA GAGATCTTCC ATTCTTTGAC AATATCTTTC 
TCTCGGTGCT GGGAATTTCT TCTCTGGATG GATCCCTCTA ACTATGGTGG AATCAAAGGA 
AATGCTCCAT CTAGTATCCA CTATGGACAG CAAGACTCCC AAAAAGGGGG CAGAGTAGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAAGAG GAGGAGGAAG GCAAACAGGA ACCAGATGTG 
GAGGACATGC TGGAAAACAA CTGGAACATT GTGCAGTTCC TGCCACAGGC AGCCTCCTGC 
CAGAGCTACT TCCTCATGAT TGTTTCAGCA TACATCGTGG CCGTGTACCA CAGCATGAAG 
GAGGAGCTGC GGCGCAGTGC CCCAGGGGGC ACACCCCTGA GATGGCGGGG GCCCAGCCAG 
CTCTCCCAGG ATGCTGCGGC CTCCAGTGCC CTTCCTGCTA TGGTCCCTGC CCTCCTAGGA 
ATGATCACCT TCTCCCAAGA CTACGTGGCC AACGAGCTGA CTCAGAACTT CTTCACCATC 
ACTCAGAAGA TTCAGAAGAA AGTCACAGGC TCCCGGAGCT CCACTGAGCT CTCCAGCATG 
TTTCCTGCCC TTCCAGGTTC TCACTTGCTG CTCAAAAACC CCGCATTGGA GTTCATCAAG 
TACGTGTGCA AGGTTCTGTC TCTGGACACG AACATCACAA ACCAGGTGAA CAAATTGAAC 
CGGGATCTGC TTCGTCTGGT GGATGTTGGC GAGTTCTCGG AGGAGGCACA GTTCCGAGAC 
CCTTGCCGTT CCTACGTGCT CCCTGAGGTC ATCTGCCGCA GCTGTAACTT CTGCCGGGAC 
CTGGACCTGT GTAAAGAGTT CGCCTTCTCG CAGGATGGGG CCGTGCTGCC TCAGTGGCTC 
TGCTCAAACT GTCAGGTTGC CTATGACACC TCAGTCATCG AGATGGCCCT GGTGGAAGCC 
CTGCAGAAGA AACTGATGGC CTTCACTCTG CAGGACCTGG CTGGGGAGAC TCCTGACCAG 
GACTGCTCCT CATTGCAGAT CTGCCTGAAG TGCCGAGGTG TGAAGGAGAC CAACATGCCT 
GTGTACTGCA GCTGCGCCGG GGGCTTTACC CTCACCATCC ACACCAAGGT CTTCATGGAG 
CAGATTGGAA TCTTCCAGAA CATTGCCCAG CACTATGGCA TGTCCTACCT CACAGAGACC 
CTGGAGTGGC TGCTGCAGAA GAACCCTCAG CTGGGCCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265954.1
CDS	16..6897
Translation	MIGEDRISFLLSFRDDGLSSSVSALKRLERSQWTDKMDLRFGFERLKESGEKTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYEPYFYIAARKGCEREVSSFLSKKFQGRIAKIETVPKEDLDLPNHLVGLKRNYIKLSFHTVEDLVRVRKDVSPAVKKNREQGHAGDAYTAMLSSVLQGGSVVIDEEEASKKVTDQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNIRYRGSAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDVEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFDYVQEMKPTIMVTYNGDFFDWPFVEARAAVHGLSMYQEIGFQKDSQGEYKASQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTGDGHVLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPMEHVTNFQEVCDQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAVVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLTAEGPARAFHELSREEQAKYEKRRLSDYCRKAYKKIHVTRVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAMEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRRGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTSVKKPKVTISYPGAMLNIMVKEGFTNDQYQELTEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEIKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPMVRKHFLRKWLKSSSLQDFDIRTILDWEYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVCKQKKISELFVLEGRRQVGMAQVPEGTQSLGAPDMEDFGLMKPLHLAVPIATKRKRVLWESQEESHDLELTVPWQEILGQPPALGTTQEEWLVWLRFHKKKWQLQARQRLACKKRRCVEVAEGEPLPGAIRERPATGLGGFLRRTARSILDLPWQIVQISETSQAGLFRLWAVISSDLYCIRLNIPRVFYVNQQVAKAEEGPSYRKVSRVLPRSNMVYNLYEYSVPEDMYQEHINEINTELSAPDIEGVYETQVPLLFRALVQLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHLYLYHHAQGHKALFGLFVPSQRRASVFVLDTVRSNQMPSLSALYSAEHSLLMEKVGPELLPPPKHTFEVRAETDMKTICRAIQRFLLAYKEERRGPTLIAVQSNWELKRLAGEVPVLEEFPLVPVHVADKISYGVLDWQRLGARRMIRHYLNLDTSLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHSHLLWLSPTSRPDLGGKEADDNRLVMEFDDQASVEINCPGCYSTVCVELDIQNLAVNTILQSHHINDMEGADSMGISFDVIQQASLEDMITGNQAASTPASYDETALCSSTFRILKSMVVGWVKEITQYRNIYADNQVMHFYRWLRSPSSLLHDPVLHRTLHNMMKKLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRIEDAIAYMEYITSSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGNAPSSIHYGQQDSQKGGRVEEEEEEEEEEEEEEGKQEPDVEDMLENNWNIVQFLPQAASCQSYFLMIVSAYIVAVYHSMKEELRRSAPGGTPLRWRGPSQLSQDAAASSALPAMVPALLGMITFSQDYVANELTQNFFTITQKIQKKVTGSRSSTELSSMFPALPGSHLLLKNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCKEFAFSQDGAVLPQWLCSNCQVAYDTSVIEMALVEALQKKLMAFTLQDLAGETPDQDCSSLQICLKCRGVKETNMPVYCSCAGGFTLTIHTKVFMEQIGIFQNIAQHYGMSYLTETLEWLLQKNPQLGH

Target ORF information:

RefSeq Version	XM_022410246.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410246.1

ORF Insert Sequence:

ATGATCGGTG AAGACAGAAT TTCCTTTCTC CTTTCATTCA GGGATGATGG CCTCTCCTCT 
TCTGTCTCAG CACTTAAGCG CCTGGAGCGC AGTCAGTGGA CAGATAAGAT GGATTTGCGG 
TTTGGTTTTG AGAGACTCAA GGAGTCTGGG GAGAAGACGG GCTGGCTTAT CAACATGCAC 
CCTACTGAGA TTTTAGATGA AGACAAACGC TTAGTCAGCG CAGTAGATTA CTACTTTATT 
CAAGATGATG GGAGCAGATT TAAGGTGGCC TTGCCCTACG AGCCGTATTT CTACATTGCA 
GCCAGAAAGG GTTGTGAACG AGAAGTTTCA TCTTTTCTCT CCAAGAAGTT TCAGGGTAGA 
ATTGCTAAAA TAGAGACTGT CCCCAAAGAG GATCTAGACT TGCCAAATCA CTTGGTGGGT 
TTGAAGCGAA ATTACATTAA GCTGTCCTTC CACACTGTGG AGGATCTTGT TAGAGTGAGG 
AAGGATGTCT CCCCTGCTGT GAAGAAGAAC CGGGAGCAGG GTCATGCTGG TGATGCCTAC 
ACAGCTATGC TGTCCAGCGT TCTGCAAGGG GGCAGTGTGG TTATTGATGA AGAGGAAGCC 
TCTAAGAAGG TCACTGACCA GTTGGACAAT ATTGTGGACA TGCGAGAGTA TGATGTGCCC 
TACCACATCC GCCTCTCCAT TGACCTGAAG ATCCATGTGG CTCATTGGTA CAACATCAGA 
TACCGAGGAA GTGCCTTTCC TGTGGAAATC ACCCGCCGAG ATGACCTTGT TGAACGACCT 
GACCCTGTGG TTTTGGCATT TGACATTGAG ACAACCAAAC TGCCTCTCAA GTTTCCTGAT 
GCTGAGACTG ACCAGATTAT GATGATTTCC TACATGATTG ATGGTCAGGG CTACCTCATC 
ACCAACAGGG AGATCGTTTC AGAAGATGTC GAAGATTTCG AGTTCACCCC CAAGCCAGAG 
TATGAAGGAC CTTTTTGTGT CTTTAATGAA CCTGACGAGG TTCATCTAAT CCAGAGATGG 
TTTGACTATG TCCAGGAGAT GAAACCCACC ATCATGGTCA CCTACAATGG GGACTTTTTT 
GACTGGCCGT TTGTGGAGGC CAGAGCAGCA GTCCACGGAC TAAGCATGTA CCAGGAGATC 
GGGTTCCAGA AGGACAGTCA GGGAGAGTAC AAGGCATCCC AGTGCATCCA CATGGACTGT 
CTCAGGTGGG TGAAGAGGGA CAGTTACCTT CCTGTGGGCA GCCACAACCT CAAGGCAGCT 
GCCAAAGCCA AGCTAGGCTA TGACCCAGTG GAGCTGGACC CTGAGGACAT GTGCCGAATG 
GCCACCGAGC AGCCCCAGAC TCTGGCCACG TACTCCGTGT CAGATGCAGT GGCCACGTAC 
TACCTGTACA TGAAATACGT CCACCCCTTT ATATTCGCCC TTTGCACCAT TATCCCCATG 
GAGCCTGATG AGGTGCTGCG GAAGGGCTCT GGGACTCTCT GTGAGGCCTT GCTGATGGTG 
CAGGCCTTCC ACGCCAACAT CATCTTCCCC AATAAGCAGG AGCAGGAGTT CAACAAGCTG 
ACAGGTGATG GCCACGTGCT GGATGCCGAG ACCTATGTGG GTGGCCACGT GGAGGCCCTT 
GAGTCAGGCG TCTTTCGCAG TGACATCCCC TGCCGGTTCA GGATGAATCC TGCTGCCTTT 
GACTTCCTGC TGCAGAGGGT GGAGAAGACC ATGCGCCATG CCATCGAGGA AGAGGAGAAG 
GTGCCCATGG AGCATGTCAC CAACTTCCAA GAGGTGTGTG ATCAGATTAA GACCAAGCTC 
ACCTCCCTGA AAGATGTTCC AAACAGAATA GAGTGTCCCC TTATCTACCA CCTGGATGTG 
GGGGCCATGT ACCCTAACAT CATCCTGACG AACCGCCTGC AGCCCTCTGC CGTGGTGGAT 
GAGGCTACCT GTGCTGCCTG TGACTTCAAT AAGCCTGGGG CCAACTGCCA GAGAAAGATG 
GCCTGGCAGT GGAGGGGCGA GTTCATGCCA GCCAGTCGCA GTGAGTACCA TCGGATCCAG 
CACCAGCTGG AGTCAGAGAA GTTCCCTCCC TTGACTGCAG AGGGTCCAGC CCGGGCCTTT 
CACGAGCTCT CTCGAGAGGA GCAGGCTAAA TATGAGAAGC GGAGGCTGTC AGATTACTGC 
CGGAAGGCAT ACAAGAAGAT ACACGTCACC AGGGTGGAGG AGCGCCTCAC CACTATCTGC 
CAGCGGGAGA ACTCTTTCTA TGTGGACACA GTGCGCGCCT TCCGAGACAG GCGTTATGAA 
TTCAAAGGTC TCCACAAGGT GTGGAAGAAG AAACTCTCTG CAGCCATGGA GGTGGGTGAT 
GCGGCCGAAG TGAAGCGCTG CAAGAACATG GAGGTCCTGT ATGACTCCCT GCAGCTGGCG 
CACAAGTGCA TCCTCAACTC CTTCTATGGC TACGTCATGC GCAGAGGGGC TCGCTGGTAC 
TCCATGGAGA TGGCAGGCAT TGTCTGCTTC ACAGGGGCTA ACATCATCAC CCAGGCACGG 
GAGCTGATTG AGCAGATCGG GAGACCCTTG GAGCTGGACA CAGACGGAAT ATGGTGCGTC 
CTGCCCAACA GCTTTCCTGA AAACTTTGTC ATCAAGACAA CCAGTGTGAA AAAGCCCAAG 
GTGACCATTT CCTACCCTGG GGCCATGTTG AACATCATGG TGAAGGAAGG CTTCACCAAT 
GATCAGTACC AGGAGCTGAC TGAACCATCC TCACTCACCT ATGTCACCCG TTCAGAGAAC 
AGCATCTTTT TCGAGGTTGA CGGACCCTAC CTTGCCATGA TCCTTCCAGC CTCCAAGGAA 
GAAGGCAAGA AACTGAAGAA GAGATATGCT GTCTTCAACG AGGACGGTTC CCTGGCTGAG 
CTGAAGGGCT TCGAGATCAA ACGCCGAGGG GAGCTGCAGC TGATTAAGAT CTTCCAGTCC 
TCAGTGTTCG AGGCTTTCCT CAAGGGCAGC ACCTTAGAAG AAGTGTATGG CTCTGTTGCC 
AAGGTGGCTG ACTACTGGCT GGACGTGCTG TACAGTAAGG CAGCTAACAT GCCCGATTCT 
GAGCTGTTTG AGCTGATCTC TGAGAATCGC TCCATGTCTC GGAAGCTGGA AGATTATGGG 
GAGCAGAAGT CCACGTCTAT CAGCACAGCC AAGCGCCTGG CTGAGTTCCT GGGAGATCAG 
ATGGTGAAGG ATGCAGGGCT GAGCTGCCGT TACATCATCT CTCGGAAGCC CGAGGGCTCT 
CCGGTCACAG AGAGGGCCAT CCCGCTTGCC ATTTTCCAGG CAGAGCCCAT GGTGAGGAAG 
CACTTTCTCC GGAAATGGCT TAAGAGTTCT TCCCTCCAAG ACTTTGATAT TCGCACGATT 
TTGGATTGGG AGTACTACAT TGAGCGGCTA GGGAGTGCCA TACAGAAGAT CATCACGATC 
CCTGCAGCTT TGCAGCAGGT AAAGAACCCA GTACCACGCG TCAAGCACCC TGACTGGCTG 
CACAAGAAAC TTTTGGAGAA GAATGATGTC TGCAAGCAGA AGAAGATCAG CGAGCTCTTT 
GTCCTCGAGG GCAGGAGACA GGTGGGCATG GCCCAGGTTC CAGAAGGCAC CCAAAGCCTG 
GGTGCTCCTG ATATGGAAGA TTTTGGCCTC ATGAAGCCAT TGCACTTGGC CGTTCCTATT 
GCCACTAAGA GGAAGCGTGT CCTCTGGGAG AGCCAGGAGG AGTCACACGA CCTGGAGCTG 
ACGGTGCCCT GGCAGGAAAT CTTGGGGCAG CCTCCAGCCC TTGGAACCAC CCAGGAAGAG 
TGGCTGGTTT GGCTCCGGTT CCATAAGAAG AAGTGGCAGC TGCAGGCCCG GCAGCGCCTG 
GCTTGCAAGA AGAGGCGGTG TGTGGAGGTG GCAGAGGGCG AACCTCTGCC TGGGGCCATC 
CGAGAAAGGC CTGCCACTGG GTTGGGGGGC TTCCTGCGAA GGACCGCCCG CAGCATCTTG 
GACCTTCCAT GGCAGATTGT GCAGATCAGT GAAACCAGCC AGGCTGGTTT ATTCAGGCTG 
TGGGCTGTCA TCAGCAGTGA CTTGTACTGC ATCAGGCTGA ACATCCCCCG AGTATTCTAT 
GTCAACCAGC AGGTGGCTAA AGCTGAGGAG GGGCCTTCCT ATCGCAAGGT CAGCCGAGTC 
CTTCCTCGCT CCAACATGGT CTACAATCTC TATGAGTACT CCGTGCCTGA GGACATGTAC 
CAGGAACATA TCAATGAAAT CAATACTGAG TTATCAGCCC CAGACATTGA GGGCGTGTAT 
GAGACTCAGG TTCCATTACT ATTCCGCGCC CTAGTGCAGC TGGGCTGTGT CTGTGTGGTC 
AATAAACAGC TGGTGAGGCA CCTTTCAGGC TGGGAAGCAG AAACCTTTGC TCTCGAGCAC 
CTGGAGATGC GTTCTCTGGC TCAGTTCAGC TACCTGGAAC CAGGGAGCAT CCGCCACCTC 
TACCTGTACC ATCATGCACA GGGCCACAAA GCACTCTTCG GCTTGTTCGT GCCCTCCCAG 
CGCAGAGCAT CTGTGTTTGT GTTGGACACT GTGCGAAGCA ACCAGATGCC CAGCCTCAGC 
GCTCTTTACT CAGCCGAGCA CAGCCTTCTG ATGGAGAAAG TGGGCCCGGA GCTCCTGCCT 
CCTCCCAAAC ACACTTTTGA AGTTCGGGCG GAAACTGACA TGAAAACCAT CTGCAGAGCC 
ATCCAGCGCT TCCTGCTGGC CTACAAAGAG GAGCGCCGTG GGCCCACACT CATCGCTGTT 
CAGTCCAACT GGGAGCTAAA GAGGCTGGCT GGTGAGGTTC CTGTCCTAGA GGAATTCCCA 
CTAGTGCCTG TCCACGTGGC AGATAAGATC AGCTATGGAG TCCTGGACTG GCAGCGCCTC 
GGAGCCCGGC GCATGATCCG CCACTACCTC AACCTGGACA CCTCTCTGTC ACAGGCCTTC 
GAGATGAGCA GGTACTTCCA CATCCCCATT GGTAATCTGC CTGAGGACAT CTCCACCTTT 
GGCTCCGACC TCTTCTTTGC CCGCCATCTC CAGCGCCACA GCCACCTGCT CTGGCTGTCG 
CCTACGTCAC GCCCTGACCT GGGTGGCAAG GAGGCTGATG ACAATCGCCT TGTCATGGAA 
TTCGATGACC AGGCCAGCGT GGAGATCAAT TGTCCAGGCT GCTACTCTAC AGTGTGTGTG 
GAGCTGGACA TCCAAAACCT GGCTGTCAAC ACCATCCTGC AGTCCCACCA TATCAATGAC 
ATGGAGGGGG CTGACAGCAT GGGCATCAGC TTTGATGTGA TCCAGCAAGC CTCTCTGGAG 
GACATGATCA CTGGCAACCA GGCCGCCAGC ACTCCAGCCA GCTACGATGA GACAGCCCTC 
TGCTCCAGCA CCTTCAGGAT CCTGAAGAGC ATGGTGGTGG GCTGGGTGAA GGAGATCACA 
CAGTACCGCA ACATCTATGC TGACAACCAG GTGATGCACT TTTACCGCTG GCTCCGGTCC 
CCATCATCAC TGCTCCATGA CCCTGTGCTG CATCGCACAC TCCACAACAT GATGAAGAAG 
CTCTTTCTGC AGCTCATCGC TGAGTTCAAG CGCTTGGGGT CATCTGTCGT CTATGCCAAC 
TTCAACCGCA TCATCCTCTG CACGAAAAAG CGGCGGATCG AGGATGCCAT TGCTTACATG 
GAGTACATCA CCAGCAGCAT CCATTCTAAA GAGATCTTCC ATTCTTTGAC AATATCTTTC 
TCTCGGTGCT GGGAATTTCT TCTCTGGATG GATCCCTCTA ACTATGGTGG AATCAAAGGA 
AATGCTCCAT CTAGTATCCA CTATGGACAG CAAGACTCCC AAAAAGGGGG CAGAGTAGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAAGAG GAGGAGGAAG GCAAACAGGA ACCAGATGTG 
GAGGACATGC TGGAAAACAA CTGGAACATT GTGCAGTTCC TGCCACAGGC AGCCTCCTGC 
CAGAGCTACT TCCTCATGAT TGTTTCAGCA TACATCGTGG CCGTGTACCA CAGCATGAAG 
GAGGAGCTGC GGCGCAGTGC CCCAGGGGGC ACACCCCTGA GATGGCGGGG GCCCAGCCAG 
CTCTCCCAGG ATGCTGCGGC CTCCAGTGCC CTTCCTGCTA TGGTCCCTGC CCTCCTAGGA 
ATGATCACCT TCTCCCAAGA CTACGTGGCC AACGAGCTGA CTCAGAACTT CTTCACCATC 
ACTCAGAAGA TTCAGAAGAA AGTCACAGGC TCCCGGAGCT CCACTGAGCT CTCCAGCATG 
TTTCCTGCCC TTCCAGGTTC TCACTTGCTG CTCAAAAACC CCGCATTGGA GTTCATCAAG 
TACGTGTGCA AGGTTCTGTC TCTGGACACG AACATCACAA ACCAGGTGAA CAAATTGAAC 
CGGGATCTGC TTCGTCTGGT GGATGTTGGC GAGTTCTCGG AGGAGGCACA GTTCCGAGAC 
CCTTGCCGTT CCTACGTGCT CCCTGAGGTC ATCTGCCGCA GCTGTAACTT CTGCCGGGAC 
CTGGACCTGT GTAAAGAGTT CGCCTTCTCG CAGGATGGGG CCGTGCTGCC TCAGTGGCTC 
TGCTCAAACT GTCAGGTTGC CTATGACACC TCAGTCATCG AGATGGCCCT GGTGGAAGCC 
CTGCAGAAGA AACTGATGGC CTTCACTCTG CAGGACCTGG CTGGGGAGAC TCCTGACCAG 
GACTGCTCCT CATTGCAGAT CTGCCTGAAG TGCCGAGGTG TGAAGGAGAC CAACATGCCT 
GTGTACTGCA GCTGCGCCGG GGGCTTTACC CTCACCATCC ACACCAAGGT CTTCATGGAG 
CAGATTGGAA TCTTCCAGAA CATTGCCCAG CACTATGGCA TGTCCTACCT CACAGAGACC 
CTGGAGTGGC TGCTGCAGAA GAACCCTCAG CTGGGCCACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_022410247.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265955.1 Canis lupus familiaris DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.

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CloneID	OCe297420
Clone ID Related Accession (Same CDS sequence)	XM_022410247.1
Accession Version	XM_022410247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DNA polymerase epsilon catalytic subunit A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGGTG AAGACAGAAT TTCCTTTCTC CTTTCATTCA GGGATGATGG CCTCTCCTCT 
TCTGTCTCAG CACTTAAGCG CCTGGAGCGC AGTCAGTGGA CAGATAAGAT GGATTTGCGG 
TTTGGTTTTG AGAGACTCAA GGAGTCTGGG GAGAAGACGG GCTGGCTTAT CAACATGCAC 
CCTACTGAGA TTTTAGATGA AGACAAACGC TTAGTCAGCG CAGTAGATTA CTACTTTATT 
CAAGATGATG GGAGCAGATT TAAGGTGGCC TTGCCCTACG AGCCGTATTT CTACATTGCA 
GCCAGAAAGG GTTGTGAACG AGAAGTTTCA TCTTTTCTCT CCAAGAAGTT TCAGGGTAGA 
ATTGCTAAAA TAGAGACTGT CCCCAAAGAG GATCTAGACT TGCCAAATCA CTTGGTGGGT 
TTGAAGCGAA ATTACATTAA GCTGTCCTTC CACACTGTGG AGGATCTTGT TAGAGTGAGG 
AAGGATGTCT CCCCTGCTGT GAAGAAGAAC CGGGAGCAGG GTCATGCTGG TGATGCCTAC 
ACAGCTATGC TGTCCAGCGT TCTGCAAGGG GGCAGTGTGG TTATTGATGA AGAGGAAGCC 
TCTAAGAAGG TCACTGACCA GTTGGACAAT ATTGTGGACA TGCGAGAGTA TGATGTGCCC 
TACCACATCC GCCTCTCCAT TGACCTGAAG ATCCATGTGG CTCATTGGTA CAACATCAGA 
TACCGAGGAA GTGCCTTTCC TGTGGAAATC ACCCGCCGAG ATGACCTTGT TGAACGACCT 
GACCCTGTGG TTTTGGCATT TGACATTGAG ACAACCAAAC TGCCTCTCAA GTTTCCTGAT 
GCTGAGACTG ACCAGATTAT GATGATTTCC TACATGATTG ATGGTCAGGG CTACCTCATC 
ACCAACAGGG AGATCGTTTC AGAAGATGTC GAAGATTTCG AGTTCACCCC CAAGCCAGAG 
TATGAAGGAC CTTTTTGTGT CTTTAATGAA CCTGACGAGG TTCATCTAAT CCAGAGATGG 
TTTGACTATG TCCAGGAGAT GAAACCCACC ATCATGGTCA CCTACAATGG GGACTTTTTT 
GACTGGCCGT TTGTGGAGGC CAGAGCAGCA GTCCACGGAC TAAGCATGTA CCAGGAGATC 
GGGTTCCAGA AGGACAGTCA GGGAGAGTAC AAGGCATCCC AGTGCATCCA CATGGACTGT 
CTCAGGTGGG TGAAGAGGGA CAGTTACCTT CCTGTGGGCA GCCACAACCT CAAGGCAGCT 
GCCAAAGCCA AGCTAGGCTA TGACCCAGTG GAGCTGGACC CTGAGGACAT GTGCCGAATG 
GCCACCGAGC AGCCCCAGAC TCTGGCCACG TACTCCGTGT CAGATGCAGT GGCCACGTAC 
TACCTGTACA TGAAATACGT CCACCCCTTT ATATTCGCCC TTTGCACCAT TATCCCCATG 
GAGCCTGATG AGGTGCTGCG GAAGGGCTCT GGGACTCTCT GTGAGGCCTT GCTGATGGTG 
CAGGCCTTCC ACGCCAACAT CATCTTCCCC AATAAGCAGG AGCAGGAGTT CAACAAGCTG 
ACAGGTGATG GCCACGTGCT GGATGCCGAG ACCTATGTGG GTGGCCACGT GGAGGCCCTT 
GAGTCAGGCG TCTTTCGCAG TGACATCCCC TGCCGGTTCA GGATGAATCC TGCTGCCTTT 
GACTTCCTGC TGCAGAGGGT GGAGAAGACC ATGCGCCATG CCATCGAGGA AGAGGAGAAG 
GTGCCCATGG AGCATGTCAC CAACTTCCAA GAGGTGTGTG ATCAGATTAA GACCAAGCTC 
ACCTCCCTGA AAGATGTTCC AAACAGAATA GAGTGTCCCC TTATCTACCA CCTGGATGTG 
GGGGCCATGT ACCCTAACAT CATCCTGACG AACCGCCTGC AGCCCTCTGC CGTGGTGGAT 
GAGGCTACCT GTGCTGCCTG TGACTTCAAT AAGCCTGGGG CCAACTGCCA GAGAAAGATG 
GCCTGGCAGT GGAGGGGCGA GTTCATGCCA GCCAGTCGCA GTGAGTACCA TCGGATCCAG 
CACCAGCTGG AGTCAGAGAA GTTCCCTCCC TTGACTGCAG AGGGTCCAGC CCGGGCCTTT 
CACGAGCTCT CTCGAGAGGA GCAGGCTAAA TATGAGAAGC GGAGGCTGTC AGATTACTGC 
CGGAAGGCAT ACAAGAAGAT ACACGTCACC AGGGTGGAGG AGCGCCTCAC CACTATCTGC 
CAGCGGGAGA ACTCTTTCTA TGTGGACACA GTGCGCGCCT TCCGAGACAG GCGTTATGAA 
TTCAAAGGTC TCCACAAGGT GTGGAAGAAG AAACTCTCTG CAGCCATGGA GGTGGGTGAT 
GCGGCCGAAG TGAAGCGCTG CAAGAACATG GAGGTCCTGT ATGACTCCCT GCAGCTGGCG 
CACAAGTGCA TCCTCAACTC CTTCTATGGC TACGTCATGC GCAGAGGGGC TCGCTGGTAC 
TCCATGGAGA TGGCAGGCAT TGTCTGCTTC ACAGGGGCTA ACATCATCAC CCAGGCACGG 
GAGCTGATTG AGCAGATCGG GAGACCCTTG GAGCTGGACA CAGACGGAAT ATGGTGCGTC 
CTGCCCAACA GCTTTCCTGA AAACTTTGTC ATCAAGACAA CCAGTGTGAA AAAGCCCAAG 
GTGACCATTT CCTACCCTGG GGCCATGTTG AACATCATGG TGAAGGAAGG CTTCACCAAT 
GATCAGTACC AGGAGCTGAC TGAACCATCC TCACTCACCT ATGTCACCCG TTCAGAGAAC 
AGCATCTTTT TCGAGGTTGA CGGACCCTAC CTTGCCATGA TCCTTCCAGC CTCCAAGGAA 
GAAGGCAAGA AACTGAAGAA GAGATATGCT GTCTTCAACG AGGACGGTTC CCTGGCTGAG 
CTGAAGGGCT TCGAGATCAA ACGCCGAGGG GAGCTGCAGC TGATTAAGAT CTTCCAGTCC 
TCAGTGTTCG AGGCTTTCCT CAAGGGCAGC ACCTTAGAAG AAGTGTATGG CTCTGTTGCC 
AAGGTGGCTG ACTACTGGCT GGACGTGCTG TACAGTAAGG CAGCTAACAT GCCCGATTCT 
GAGCTGTTTG AGCTGATCTC TGAGAATCGC TCCATGTCTC GGAAGCTGGA AGATTATGGG 
GAGCAGAAGT CCACGTCTAT CAGCACAGCC AAGCGCCTGG CTGAGTTCCT GGGAGATCAG 
ATGGTGAAGG ATGCAGGGCT GAGCTGCCGT TACATCATCT CTCGGAAGCC CGAGGGCTCT 
CCGGTCACAG AGAGGGCCAT CCCGCTTGCC ATTTTCCAGG CAGAGCCCAT GGTGAGGAAG 
CACTTTCTCC GGAAATGGCT TAAGAGTTCT TCCCTCCAAG ACTTTGATAT TCGCACGATT 
TTGGATTGGG AGTACTACAT TGAGCGGCTA GGGAGTGCCA TACAGAAGAT CATCACGATC 
CCTGCAGCTT TGCAGCAGGT AAAGAACCCA GTACCACGCG TCAAGCACCC TGACTGGCTG 
CACAAGAAAC TTTTGGAGAA GAATGATGTC TGCAAGCAGA AGAAGATCAG CGAGCTCTTT 
GTCCTCGAGG GCAGGAGACA GGTGGGCATG GCCCAGGTTC CAGAAGGCAC CCAAAGCCTG 
GGTGCTCCTG ATATGGAAGA TTTTGGCCTC ATGAAGCCAT TGCACTTGGC CGTTCCTATT 
GCCACTAAGA GGAAGCGTGT CCTCTGGGAG AGCCAGGAGG AGTCACACGA CCTGGAGCTG 
ACGGTGCCCT GGCAGGAAAT CTTGGGGCAG CCTCCAGCCC TTGGAACCAC CCAGGAAGAG 
TGGCTGGTTT GGCTCCGGTT CCATAAGAAG AAGTGGCAGC TGCAGGCCCG GCAGCGCCTG 
GCTTGCAAGA AGAGGCGGTG TGTGGAGGTG GCAGAGGGCG AACCTCTGCC TGGGGCCATC 
CGAGAAAGGC CTGCCACTGG GTTGGGGGGC TTCCTGCGAA GGACCGCCCG CAGCATCTTG 
GACCTTCCAT GGCAGATTGT GCAGATCAGT GAAACCAGCC AGGCTGGTTT ATTCAGGCTG 
TGGGCTGTCA TCAGCAGTGA CTTGTACTGC ATCAGGCTGA ACATCCCCCG AGTATTCTAT 
GTCAACCAGC AGGTGGCTAA AGCTGAGGAG GGGCCTTCCT ATCGCAAGGT CAGCCGAGTC 
CTTCCTCGCT CCAACATGGT CTACAATCTC TATGAGTACT CCGTGCCTGA GGACATGTAC 
CAGGAACATA TCAATGAAAT CAATACTGAG TTATCAGCCC CAGACATTGA GGGCGTGTAT 
GAGACTCAGG TTCCATTACT ATTCCGCGCC CTAGTGCAGC TGGGCTGTGT CTGTGTGGTC 
AATAAACAGC TGGTGAGGCA CCTTTCAGGC TGGGAAGCAG AAACCTTTGC TCTCGAGCAC 
CTGGAGATGC GTTCTCTGGC TCAGTTCAGC TACCTGGAAC CAGGGAGCAT CCGCCACCTC 
TACCTGTACC ATCATGCACA GGGCCACAAA GCACTCTTCG GCTTGTTCGT GCCCTCCCAG 
CGCAGAGCAT CTGTGTTTGT GTTGGACACT GTGCGAAGCA ACCAGATGCC CAGCCTCAGC 
GCTCTTTACT CAGCCGAGCA CAGCCTTCTG ATGGAGAAAG TGGGCCCGGA GCTCCTGCCT 
CCTCCCAAAC ACACTTTTGA AGTTCGGGCG GAAACTGACA TGAAAACCAT CTGCAGAGCC 
ATCCAGCGCT TCCTGCTGGC CTACAAAGAG GAGCGCCGTG GGCCCACACT CATCGCTGTT 
CAGTCCAACT GGGAGCTAAA GAGGCTGGCT GGTGAGGTTC CTGTCCTAGA GGAATTCCCA 
CTAGTGCCTG TCCACGTGGC AGATAAGATC AGCTATGGAG TCCTGGACTG GCAGCGCCTC 
GGAGCCCGGC GCATGATCCG CCACTACCTC AACCTGGACA CCTCTCTGTC ACAGGCCTTC 
GAGATGAGCA GGTACTTCCA CATCCCCATT GGTAATCTGC CTGAGGACAT CTCCACCTTT 
GGCTCCGACC TCTTCTTTGC CCGCCATCTC CAGCGCCACA GCCACCTGCT CTGGCTGTCG 
CCTACGTCAC GCCCTGACCT GGGTGGCAAG GAGGCTGATG ACAATCGCCT TGTCATGGAA 
TTCGATGACC AGGCCAGCGT GGAGATCAAT TGTCCAGGCT GCTACTCTAC AGTGTGTGTG 
GAGCTGGACA TCCAAAACCT GGCTGTCAAC ACCATCCTGC AGTCCCACCA TATCAATGAC 
ATGGAGGGGG CTGACAGCAT GGGCATCAGC TTTGATGTGA TCCAGCAAGC CTCTCTGGAG 
GACATGATCA CTGGCAACCA GGCCGCCAGC ACTCCAGCCA GCTACGATGA GACAGCCCTC 
TGCTCCAGCA CCTTCAGGAT CCTGAAGAGC ATGGTGGTGG GCTGGGTGAA GGAGATCACA 
CAGTACCGCA ACATCTATGC TGACAACCAG GTGATGCACT TTTACCGCTG GCTCCGGTCC 
CCATCATCAC TGCTCCATGA CCCTGTGCTG CATCGCACAC TCCACAACAT GATGAAGAAG 
CTCTTTCTGC AGCTCATCGC TGAGTTCAAG CGCTTGGGGT CATCTGTCGT CTATGCCAAC 
TTCAACCGCA TCATCCTCTG CACGAAAAAG CGGCGGATCG AGGATGCCAT TGCTTACATG 
GAGTACATCA CCAGCAGCAT CCATTCTAAA GAGATCTTCC ATTCTTTGAC AATATCTTTC 
TCTCGGTGCT GGGAATTTCT TCTCTGGATG GATCCCTCTA ACTATGGTGG AATCAAAGGA 
AATGCTCCAT CTAGTATCCA CTATGGACAG CAAGACTCCC AAAAAGGGGG CAGAGTAGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAAGAG GAGGAGGAAG GCAAACAGGA ACCAGATGTG 
GAGGACATGC TGGAAAACAA CTGGAACATT GTGCAGTTCC TGCCACAGGC AGCCTCCTGC 
CAGAGCTACT TCCTCATGAT TGTTTCAGCA TACATCGTGG CCGTGTACCA CAGCATGAAG 
GAGGAGCTGC GGCGCAGTGC CCCAGGGGGC ACACCCCTGA GATGGCGGGG GCCCAGCCAG 
CTCTCCCAGG ATGCTGCGGC CTCCAGTGCC CTTCCTGGAA TGATCACCTT CTCCCAAGAC 
TACGTGGCCA ACGAGCTGAC TCAGAACTTC TTCACCATCA CTCAGAAGAT TCAGAAGAAA 
GTCACAGGCT CCCGGAGCTC CACTGAGCTC TCCAGCATGT TTCCTGCCCT TCCAGGTTCT 
CACTTGCTGC TCAAAAACCC CGCATTGGAG TTCATCAAGT ACGTGTGCAA GGTTCTGTCT 
CTGGACACGA ACATCACAAA CCAGGTGAAC AAATTGAACC GGGATCTGCT TCGTCTGGTG 
GATGTTGGCG AGTTCTCGGA GGAGGCACAG TTCCGAGACC CTTGCCGTTC CTACGTGCTC 
CCTGAGGTCA TCTGCCGCAG CTGTAACTTC TGCCGGGACC TGGACCTGTG TAAAGAGTTC 
GCCTTCTCGC AGGATGGGGC CGTGCTGCCT CAGTGGCTCT GCTCAAACTG TCAGGTTGCC 
TATGACACCT CAGTCATCGA GATGGCCCTG GTGGAAGCCC TGCAGAAGAA ACTGATGGCC 
TTCACTCTGC AGGACCTGGC TGGGGAGACT CCTGACCAGG ACTGCTCCTC ATTGCAGATC 
TGCCTGAAGT GCCGAGGTGT GAAGGAGACC AACATGCCTG TGTACTGCAG CTGCGCCGGG 
GGCTTTACCC TCACCATCCA CACCAAGGTC TTCATGGAGC AGATTGGAAT CTTCCAGAAC 
ATTGCCCAGC ACTATGGCAT GTCCTACCTC ACAGAGACCC TGGAGTGGCT GCTGCAGAAG 
AACCCTCAGC TGGGCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265955.1
CDS	16..6876
Translation	MIGEDRISFLLSFRDDGLSSSVSALKRLERSQWTDKMDLRFGFERLKESGEKTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYEPYFYIAARKGCEREVSSFLSKKFQGRIAKIETVPKEDLDLPNHLVGLKRNYIKLSFHTVEDLVRVRKDVSPAVKKNREQGHAGDAYTAMLSSVLQGGSVVIDEEEASKKVTDQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNIRYRGSAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDVEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFDYVQEMKPTIMVTYNGDFFDWPFVEARAAVHGLSMYQEIGFQKDSQGEYKASQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTGDGHVLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPMEHVTNFQEVCDQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAVVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLTAEGPARAFHELSREEQAKYEKRRLSDYCRKAYKKIHVTRVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAMEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRRGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTSVKKPKVTISYPGAMLNIMVKEGFTNDQYQELTEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEIKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPMVRKHFLRKWLKSSSLQDFDIRTILDWEYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVCKQKKISELFVLEGRRQVGMAQVPEGTQSLGAPDMEDFGLMKPLHLAVPIATKRKRVLWESQEESHDLELTVPWQEILGQPPALGTTQEEWLVWLRFHKKKWQLQARQRLACKKRRCVEVAEGEPLPGAIRERPATGLGGFLRRTARSILDLPWQIVQISETSQAGLFRLWAVISSDLYCIRLNIPRVFYVNQQVAKAEEGPSYRKVSRVLPRSNMVYNLYEYSVPEDMYQEHINEINTELSAPDIEGVYETQVPLLFRALVQLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHLYLYHHAQGHKALFGLFVPSQRRASVFVLDTVRSNQMPSLSALYSAEHSLLMEKVGPELLPPPKHTFEVRAETDMKTICRAIQRFLLAYKEERRGPTLIAVQSNWELKRLAGEVPVLEEFPLVPVHVADKISYGVLDWQRLGARRMIRHYLNLDTSLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHSHLLWLSPTSRPDLGGKEADDNRLVMEFDDQASVEINCPGCYSTVCVELDIQNLAVNTILQSHHINDMEGADSMGISFDVIQQASLEDMITGNQAASTPASYDETALCSSTFRILKSMVVGWVKEITQYRNIYADNQVMHFYRWLRSPSSLLHDPVLHRTLHNMMKKLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRIEDAIAYMEYITSSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGNAPSSIHYGQQDSQKGGRVEEEEEEEEEEEEEEGKQEPDVEDMLENNWNIVQFLPQAASCQSYFLMIVSAYIVAVYHSMKEELRRSAPGGTPLRWRGPSQLSQDAAASSALPGMITFSQDYVANELTQNFFTITQKIQKKVTGSRSSTELSSMFPALPGSHLLLKNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCKEFAFSQDGAVLPQWLCSNCQVAYDTSVIEMALVEALQKKLMAFTLQDLAGETPDQDCSSLQICLKCRGVKETNMPVYCSCAGGFTLTIHTKVFMEQIGIFQNIAQHYGMSYLTETLEWLLQKNPQLGH

Target ORF information:

RefSeq Version	XM_022410247.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410247.1

ORF Insert Sequence:

ATGATCGGTG AAGACAGAAT TTCCTTTCTC CTTTCATTCA GGGATGATGG CCTCTCCTCT 
TCTGTCTCAG CACTTAAGCG CCTGGAGCGC AGTCAGTGGA CAGATAAGAT GGATTTGCGG 
TTTGGTTTTG AGAGACTCAA GGAGTCTGGG GAGAAGACGG GCTGGCTTAT CAACATGCAC 
CCTACTGAGA TTTTAGATGA AGACAAACGC TTAGTCAGCG CAGTAGATTA CTACTTTATT 
CAAGATGATG GGAGCAGATT TAAGGTGGCC TTGCCCTACG AGCCGTATTT CTACATTGCA 
GCCAGAAAGG GTTGTGAACG AGAAGTTTCA TCTTTTCTCT CCAAGAAGTT TCAGGGTAGA 
ATTGCTAAAA TAGAGACTGT CCCCAAAGAG GATCTAGACT TGCCAAATCA CTTGGTGGGT 
TTGAAGCGAA ATTACATTAA GCTGTCCTTC CACACTGTGG AGGATCTTGT TAGAGTGAGG 
AAGGATGTCT CCCCTGCTGT GAAGAAGAAC CGGGAGCAGG GTCATGCTGG TGATGCCTAC 
ACAGCTATGC TGTCCAGCGT TCTGCAAGGG GGCAGTGTGG TTATTGATGA AGAGGAAGCC 
TCTAAGAAGG TCACTGACCA GTTGGACAAT ATTGTGGACA TGCGAGAGTA TGATGTGCCC 
TACCACATCC GCCTCTCCAT TGACCTGAAG ATCCATGTGG CTCATTGGTA CAACATCAGA 
TACCGAGGAA GTGCCTTTCC TGTGGAAATC ACCCGCCGAG ATGACCTTGT TGAACGACCT 
GACCCTGTGG TTTTGGCATT TGACATTGAG ACAACCAAAC TGCCTCTCAA GTTTCCTGAT 
GCTGAGACTG ACCAGATTAT GATGATTTCC TACATGATTG ATGGTCAGGG CTACCTCATC 
ACCAACAGGG AGATCGTTTC AGAAGATGTC GAAGATTTCG AGTTCACCCC CAAGCCAGAG 
TATGAAGGAC CTTTTTGTGT CTTTAATGAA CCTGACGAGG TTCATCTAAT CCAGAGATGG 
TTTGACTATG TCCAGGAGAT GAAACCCACC ATCATGGTCA CCTACAATGG GGACTTTTTT 
GACTGGCCGT TTGTGGAGGC CAGAGCAGCA GTCCACGGAC TAAGCATGTA CCAGGAGATC 
GGGTTCCAGA AGGACAGTCA GGGAGAGTAC AAGGCATCCC AGTGCATCCA CATGGACTGT 
CTCAGGTGGG TGAAGAGGGA CAGTTACCTT CCTGTGGGCA GCCACAACCT CAAGGCAGCT 
GCCAAAGCCA AGCTAGGCTA TGACCCAGTG GAGCTGGACC CTGAGGACAT GTGCCGAATG 
GCCACCGAGC AGCCCCAGAC TCTGGCCACG TACTCCGTGT CAGATGCAGT GGCCACGTAC 
TACCTGTACA TGAAATACGT CCACCCCTTT ATATTCGCCC TTTGCACCAT TATCCCCATG 
GAGCCTGATG AGGTGCTGCG GAAGGGCTCT GGGACTCTCT GTGAGGCCTT GCTGATGGTG 
CAGGCCTTCC ACGCCAACAT CATCTTCCCC AATAAGCAGG AGCAGGAGTT CAACAAGCTG 
ACAGGTGATG GCCACGTGCT GGATGCCGAG ACCTATGTGG GTGGCCACGT GGAGGCCCTT 
GAGTCAGGCG TCTTTCGCAG TGACATCCCC TGCCGGTTCA GGATGAATCC TGCTGCCTTT 
GACTTCCTGC TGCAGAGGGT GGAGAAGACC ATGCGCCATG CCATCGAGGA AGAGGAGAAG 
GTGCCCATGG AGCATGTCAC CAACTTCCAA GAGGTGTGTG ATCAGATTAA GACCAAGCTC 
ACCTCCCTGA AAGATGTTCC AAACAGAATA GAGTGTCCCC TTATCTACCA CCTGGATGTG 
GGGGCCATGT ACCCTAACAT CATCCTGACG AACCGCCTGC AGCCCTCTGC CGTGGTGGAT 
GAGGCTACCT GTGCTGCCTG TGACTTCAAT AAGCCTGGGG CCAACTGCCA GAGAAAGATG 
GCCTGGCAGT GGAGGGGCGA GTTCATGCCA GCCAGTCGCA GTGAGTACCA TCGGATCCAG 
CACCAGCTGG AGTCAGAGAA GTTCCCTCCC TTGACTGCAG AGGGTCCAGC CCGGGCCTTT 
CACGAGCTCT CTCGAGAGGA GCAGGCTAAA TATGAGAAGC GGAGGCTGTC AGATTACTGC 
CGGAAGGCAT ACAAGAAGAT ACACGTCACC AGGGTGGAGG AGCGCCTCAC CACTATCTGC 
CAGCGGGAGA ACTCTTTCTA TGTGGACACA GTGCGCGCCT TCCGAGACAG GCGTTATGAA 
TTCAAAGGTC TCCACAAGGT GTGGAAGAAG AAACTCTCTG CAGCCATGGA GGTGGGTGAT 
GCGGCCGAAG TGAAGCGCTG CAAGAACATG GAGGTCCTGT ATGACTCCCT GCAGCTGGCG 
CACAAGTGCA TCCTCAACTC CTTCTATGGC TACGTCATGC GCAGAGGGGC TCGCTGGTAC 
TCCATGGAGA TGGCAGGCAT TGTCTGCTTC ACAGGGGCTA ACATCATCAC CCAGGCACGG 
GAGCTGATTG AGCAGATCGG GAGACCCTTG GAGCTGGACA CAGACGGAAT ATGGTGCGTC 
CTGCCCAACA GCTTTCCTGA AAACTTTGTC ATCAAGACAA CCAGTGTGAA AAAGCCCAAG 
GTGACCATTT CCTACCCTGG GGCCATGTTG AACATCATGG TGAAGGAAGG CTTCACCAAT 
GATCAGTACC AGGAGCTGAC TGAACCATCC TCACTCACCT ATGTCACCCG TTCAGAGAAC 
AGCATCTTTT TCGAGGTTGA CGGACCCTAC CTTGCCATGA TCCTTCCAGC CTCCAAGGAA 
GAAGGCAAGA AACTGAAGAA GAGATATGCT GTCTTCAACG AGGACGGTTC CCTGGCTGAG 
CTGAAGGGCT TCGAGATCAA ACGCCGAGGG GAGCTGCAGC TGATTAAGAT CTTCCAGTCC 
TCAGTGTTCG AGGCTTTCCT CAAGGGCAGC ACCTTAGAAG AAGTGTATGG CTCTGTTGCC 
AAGGTGGCTG ACTACTGGCT GGACGTGCTG TACAGTAAGG CAGCTAACAT GCCCGATTCT 
GAGCTGTTTG AGCTGATCTC TGAGAATCGC TCCATGTCTC GGAAGCTGGA AGATTATGGG 
GAGCAGAAGT CCACGTCTAT CAGCACAGCC AAGCGCCTGG CTGAGTTCCT GGGAGATCAG 
ATGGTGAAGG ATGCAGGGCT GAGCTGCCGT TACATCATCT CTCGGAAGCC CGAGGGCTCT 
CCGGTCACAG AGAGGGCCAT CCCGCTTGCC ATTTTCCAGG CAGAGCCCAT GGTGAGGAAG 
CACTTTCTCC GGAAATGGCT TAAGAGTTCT TCCCTCCAAG ACTTTGATAT TCGCACGATT 
TTGGATTGGG AGTACTACAT TGAGCGGCTA GGGAGTGCCA TACAGAAGAT CATCACGATC 
CCTGCAGCTT TGCAGCAGGT AAAGAACCCA GTACCACGCG TCAAGCACCC TGACTGGCTG 
CACAAGAAAC TTTTGGAGAA GAATGATGTC TGCAAGCAGA AGAAGATCAG CGAGCTCTTT 
GTCCTCGAGG GCAGGAGACA GGTGGGCATG GCCCAGGTTC CAGAAGGCAC CCAAAGCCTG 
GGTGCTCCTG ATATGGAAGA TTTTGGCCTC ATGAAGCCAT TGCACTTGGC CGTTCCTATT 
GCCACTAAGA GGAAGCGTGT CCTCTGGGAG AGCCAGGAGG AGTCACACGA CCTGGAGCTG 
ACGGTGCCCT GGCAGGAAAT CTTGGGGCAG CCTCCAGCCC TTGGAACCAC CCAGGAAGAG 
TGGCTGGTTT GGCTCCGGTT CCATAAGAAG AAGTGGCAGC TGCAGGCCCG GCAGCGCCTG 
GCTTGCAAGA AGAGGCGGTG TGTGGAGGTG GCAGAGGGCG AACCTCTGCC TGGGGCCATC 
CGAGAAAGGC CTGCCACTGG GTTGGGGGGC TTCCTGCGAA GGACCGCCCG CAGCATCTTG 
GACCTTCCAT GGCAGATTGT GCAGATCAGT GAAACCAGCC AGGCTGGTTT ATTCAGGCTG 
TGGGCTGTCA TCAGCAGTGA CTTGTACTGC ATCAGGCTGA ACATCCCCCG AGTATTCTAT 
GTCAACCAGC AGGTGGCTAA AGCTGAGGAG GGGCCTTCCT ATCGCAAGGT CAGCCGAGTC 
CTTCCTCGCT CCAACATGGT CTACAATCTC TATGAGTACT CCGTGCCTGA GGACATGTAC 
CAGGAACATA TCAATGAAAT CAATACTGAG TTATCAGCCC CAGACATTGA GGGCGTGTAT 
GAGACTCAGG TTCCATTACT ATTCCGCGCC CTAGTGCAGC TGGGCTGTGT CTGTGTGGTC 
AATAAACAGC TGGTGAGGCA CCTTTCAGGC TGGGAAGCAG AAACCTTTGC TCTCGAGCAC 
CTGGAGATGC GTTCTCTGGC TCAGTTCAGC TACCTGGAAC CAGGGAGCAT CCGCCACCTC 
TACCTGTACC ATCATGCACA GGGCCACAAA GCACTCTTCG GCTTGTTCGT GCCCTCCCAG 
CGCAGAGCAT CTGTGTTTGT GTTGGACACT GTGCGAAGCA ACCAGATGCC CAGCCTCAGC 
GCTCTTTACT CAGCCGAGCA CAGCCTTCTG ATGGAGAAAG TGGGCCCGGA GCTCCTGCCT 
CCTCCCAAAC ACACTTTTGA AGTTCGGGCG GAAACTGACA TGAAAACCAT CTGCAGAGCC 
ATCCAGCGCT TCCTGCTGGC CTACAAAGAG GAGCGCCGTG GGCCCACACT CATCGCTGTT 
CAGTCCAACT GGGAGCTAAA GAGGCTGGCT GGTGAGGTTC CTGTCCTAGA GGAATTCCCA 
CTAGTGCCTG TCCACGTGGC AGATAAGATC AGCTATGGAG TCCTGGACTG GCAGCGCCTC 
GGAGCCCGGC GCATGATCCG CCACTACCTC AACCTGGACA CCTCTCTGTC ACAGGCCTTC 
GAGATGAGCA GGTACTTCCA CATCCCCATT GGTAATCTGC CTGAGGACAT CTCCACCTTT 
GGCTCCGACC TCTTCTTTGC CCGCCATCTC CAGCGCCACA GCCACCTGCT CTGGCTGTCG 
CCTACGTCAC GCCCTGACCT GGGTGGCAAG GAGGCTGATG ACAATCGCCT TGTCATGGAA 
TTCGATGACC AGGCCAGCGT GGAGATCAAT TGTCCAGGCT GCTACTCTAC AGTGTGTGTG 
GAGCTGGACA TCCAAAACCT GGCTGTCAAC ACCATCCTGC AGTCCCACCA TATCAATGAC 
ATGGAGGGGG CTGACAGCAT GGGCATCAGC TTTGATGTGA TCCAGCAAGC CTCTCTGGAG 
GACATGATCA CTGGCAACCA GGCCGCCAGC ACTCCAGCCA GCTACGATGA GACAGCCCTC 
TGCTCCAGCA CCTTCAGGAT CCTGAAGAGC ATGGTGGTGG GCTGGGTGAA GGAGATCACA 
CAGTACCGCA ACATCTATGC TGACAACCAG GTGATGCACT TTTACCGCTG GCTCCGGTCC 
CCATCATCAC TGCTCCATGA CCCTGTGCTG CATCGCACAC TCCACAACAT GATGAAGAAG 
CTCTTTCTGC AGCTCATCGC TGAGTTCAAG CGCTTGGGGT CATCTGTCGT CTATGCCAAC 
TTCAACCGCA TCATCCTCTG CACGAAAAAG CGGCGGATCG AGGATGCCAT TGCTTACATG 
GAGTACATCA CCAGCAGCAT CCATTCTAAA GAGATCTTCC ATTCTTTGAC AATATCTTTC 
TCTCGGTGCT GGGAATTTCT TCTCTGGATG GATCCCTCTA ACTATGGTGG AATCAAAGGA 
AATGCTCCAT CTAGTATCCA CTATGGACAG CAAGACTCCC AAAAAGGGGG CAGAGTAGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAAGAG GAGGAGGAAG GCAAACAGGA ACCAGATGTG 
GAGGACATGC TGGAAAACAA CTGGAACATT GTGCAGTTCC TGCCACAGGC AGCCTCCTGC 
CAGAGCTACT TCCTCATGAT TGTTTCAGCA TACATCGTGG CCGTGTACCA CAGCATGAAG 
GAGGAGCTGC GGCGCAGTGC CCCAGGGGGC ACACCCCTGA GATGGCGGGG GCCCAGCCAG 
CTCTCCCAGG ATGCTGCGGC CTCCAGTGCC CTTCCTGGAA TGATCACCTT CTCCCAAGAC 
TACGTGGCCA ACGAGCTGAC TCAGAACTTC TTCACCATCA CTCAGAAGAT TCAGAAGAAA 
GTCACAGGCT CCCGGAGCTC CACTGAGCTC TCCAGCATGT TTCCTGCCCT TCCAGGTTCT 
CACTTGCTGC TCAAAAACCC CGCATTGGAG TTCATCAAGT ACGTGTGCAA GGTTCTGTCT 
CTGGACACGA ACATCACAAA CCAGGTGAAC AAATTGAACC GGGATCTGCT TCGTCTGGTG 
GATGTTGGCG AGTTCTCGGA GGAGGCACAG TTCCGAGACC CTTGCCGTTC CTACGTGCTC 
CCTGAGGTCA TCTGCCGCAG CTGTAACTTC TGCCGGGACC TGGACCTGTG TAAAGAGTTC 
GCCTTCTCGC AGGATGGGGC CGTGCTGCCT CAGTGGCTCT GCTCAAACTG TCAGGTTGCC 
TATGACACCT CAGTCATCGA GATGGCCCTG GTGGAAGCCC TGCAGAAGAA ACTGATGGCC 
TTCACTCTGC AGGACCTGGC TGGGGAGACT CCTGACCAGG ACTGCTCCTC ATTGCAGATC 
TGCCTGAAGT GCCGAGGTGT GAAGGAGACC AACATGCCTG TGTACTGCAG CTGCGCCGGG 
GGCTTTACCC TCACCATCCA CACCAAGGTC TTCATGGAGC AGATTGGAAT CTTCCAGAAC 
ATTGCCCAGC ACTATGGCAT GTCCTACCTC ACAGAGACCC TGGAGTGGCT GCTGCAGAAG 
AACCCTCAGC TGGGCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_022410248.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265956.1 Canis lupus familiaris DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X3, mRNA.

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CloneID	OCe297421
Clone ID Related Accession (Same CDS sequence)	XM_022410248.1
Accession Version	XM_022410248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DNA polymerase epsilon catalytic subunit A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGGTG AAGACAGAAT TTCCTTTCTC CTTTCATTCA GGGATGATGG CCTCTCCTCT 
TCTGTCTCAG CACTTAAGCG CCTGGAGCGC AGTCAGTGGA CAGATAAGAT GGATTTGCGG 
TTTGGTTTTG AGAGACTCAA GGAGTCTGGG GAGAAGACGG GCTGGCTTAT CAACATGCAC 
CCTACTGAGA TTTTAGATGA AGACAAACGC TTAGTCAGCG CAGTAGATTA CTACTTTATT 
CAAGATGATG GGAGCAGATT TAAGGTGGCC TTGCCCTACG AGCCGTATTT CTACATTGCA 
GCCAGAAAGG GTTGTGAACG AGAAGTTTCA TCTTTTCTCT CCAAGAAGTT TCAGGGTAGA 
ATTGCTAAAA TAGAGACTGT CCCCAAAGAG GATCTAGACT TGCCAAATCA CTTGGTGGGT 
TTGAAGCGAA ATTACATTAA GCTGTCCTTC CACACTGTGG AGGATCTTGT TAGAGTGAGG 
AAGGATGTCT CCCCTGCTGT GAAGAAGAAC CGGGAGCAGG GTCATGCTGG TGATGCCTAC 
ACAGCTATGC TGTCCAGCGT TCTGCAAGGG GGCAGTGTGG TTATTGATGA AGAGGAAGCC 
TCTAAGAAGG TCACTGACCA GTTGGACAAT ATTGTGGACA TGCGAGAGTA TGATGTGCCC 
TACCACATCC GCCTCTCCAT TGACCTGAAG ATCCATGTGG CTCATTGGTA CAACATCAGA 
TACCGAGGAA GTGCCTTTCC TGTGGAAATC ACCCGCCGAG ATGACCTTGT TGAACGACCT 
GACCCTGTGG TTTTGGCATT TGACATTGAG ACAACCAAAC TGCCTCTCAA GTTTCCTGAT 
GCTGAGACTG ACCAGATTAT GATGATTTCC TACATGATTG ATGGTCAGGG CTACCTCATC 
ACCAACAGGG AGATCGTTTC AGAAGATGTC GAAGATTTCG AGTTCACCCC CAAGCCAGAG 
TATGAAGGAC CTTTTTGTGT CTTTAATGAA CCTGACGAGG TTCATCTAAT CCAGAGATGG 
TTTGACTATG TCCAGGAGAT GAAACCCACC ATCATGGTCA CCTACAATGG GGACTTTTTT 
GACTGGCCGT TTGTGGAGGC CAGAGCAGCA GTCCACGGAC TAAGCATGTA CCAGGAGATC 
GGGTTCCAGA AGGACAGTCA GGGAGAGTAC AAGGCATCCC AGTGCATCCA CATGGACTGT 
CTCAGGTGGG TGAAGAGGGA CAGTTACCTT CCTGTGGGCA GCCACAACCT CAAGGCAGCT 
GCCAAAGCCA AGCTAGGCTA TGACCCAGTG GAGCTGGACC CTGAGGACAT GTGCCGAATG 
GCCACCGAGC AGCCCCAGAC TCTGGCCACG TACTCCGTGT CAGATGCAGT GGCCACGTAC 
TACCTGTACA TGAAATACGT CCACCCCTTT ATATTCGCCC TTTGCACCAT TATCCCCATG 
GAGCCTGATG AGGTGCTGCG GAAGGGCTCT GGGACTCTCT GTGAGGCCTT GCTGATGGTG 
CAGGCCTTCC ACGCCAACAT CATCTTCCCC AATAAGCAGG AGCAGGAGTT CAACAAGCTG 
ACAGGTGATG GCCACGTGCT GGATGCCGAG ACCTATGTGG GTGGCCACGT GGAGGCCCTT 
GAGTCAGGCG TCTTTCGCAG TGACATCCCC TGCCGGTTCA GGATGAATCC TGCTGCCTTT 
GACTTCCTGC TGCAGAGGGT GGAGAAGACC ATGCGCCATG CCATCGAGGA AGAGGAGAAG 
GTGCCCATGG AGCATGTCAC CAACTTCCAA GAGGTGTGTG ATCAGATTAA GACCAAGCTC 
ACCTCCCTGA AAGATGTTCC AAACAGAATA GAGTGTCCCC TTATCTACCA CCTGGATGTG 
GGGGCCATGT ACCCTAACAT CATCCTGACG AACCGCCTGC AGCCCTCTGC CGTGGTGGAT 
GAGGCTACCT GTGCTGCCTG TGACTTCAAT AAGCCTGGGG CCAACTGCCA GAGAAAGATG 
GCCTGGCAGT GGAGGGGCGA GTTCATGCCA GCCAGTCGCA GTGAGTACCA TCGGATCCAG 
CACCAGCTGG AGTCAGAGAA GTTCCCTCCC TTGACTGCAG AGGGTCCAGC CCGGGCCTTT 
CACGAGCTCT CTCGAGAGGA GCAGGCTAAA TATGAGAAGC GGAGGCTGTC AGATTACTGC 
CGGAAGGCAT ACAAGAAGAT ACACGTCACC AGGGTGGAGG AGCGCCTCAC CACTATCTGC 
CAGCGGGAGA ACTCTTTCTA TGTGGACACA GTGCGCGCCT TCCGAGACAG GCGTTATGAA 
TTCAAAGGTC TCCACAAGGT GTGGAAGAAG AAACTCTCTG CAGCCATGGA GGTGGGTGAT 
GCGGCCGAAG TGAAGCGCTG CAAGAACATG GAGGTCCTGT ATGACTCCCT GCAGCTGGCG 
CACAAGTGCA TCCTCAACTC CTTCTATGGC TACGTCATGC GCAGAGGGGC TCGCTGGTAC 
TCCATGGAGA TGGCAGGCAT TGTCTGCTTC ACAGGGGCTA ACATCATCAC CCAGGCACGG 
GAGCTGATTG AGCAGATCGG GAGACCCTTG GAGCTGGACA CAGACGGAAT ATGGTGCGTC 
CTGCCCAACA GCTTTCCTGA AAACTTTGTC ATCAAGACAA CCAGTGTGAA AAAGCCCAAG 
GTGACCATTT CCTACCCTGG GGCCATGTTG AACATCATGG TGAAGGAAGG CTTCACCAAT 
GATCAGTACC AGGAGCTGAC TGAACCATCC TCACTCACCT ATGTCACCCG TTCAGAGAAC 
AGCATCTTTT TCGAGGTTGA CGGACCCTAC CTTGCCATGA TCCTTCCAGC CTCCAAGGAA 
GAAGGCAAGA AACTGAAGAA GAGATATGCT GTCTTCAACG AGGACGGTTC CCTGGCTGAG 
CTGAAGGGCT TCGAGATCAA ACGCCGAGGG GAGCTGCAGC TGATTAAGAT CTTCCAGTCC 
TCAGTGTTCG AGGCTTTCCT CAAGGGCAGC ACCTTAGAAG AAGTGTATGG CTCTGTTGCC 
AAGGTGGCTG ACTACTGGCT GGACGTGCTG TACAGTAAGG CAGCTAACAT GCCCGATTCT 
GAGCTGTTTG AGCTGATCTC TGAGAATCGC TCCATGTCTC GGAAGCTGGA AGATTATGGG 
GAGCAGAAGT CCACGTCTAT CAGCACAGCC AAGCGCCTGG CTGAGTTCCT GGGAGATCAG 
ATGGTGAAGG ATGCAGGGCT GAGCTGCCGT TACATCATCT CTCGGAAGCC CGAGGGCTCT 
CCGGTCACAG AGAGGGCCAT CCCGCTTGCC ATTTTCCAGG CAGAGCCCAT GGTGAGGAAG 
CACTTTCTCC GGAAATGGCT TAAGAGTTCT TCCCTCCAAG ACTTTGATAT TCGCACGATT 
TTGGATTGGG AGTACTACAT TGAGCGGCTA GGGAGTGCCA TACAGAAGAT CATCACGATC 
CCTGCAGCTT TGCAGCAGGT AAAGAACCCA GTACCACGCG TCAAGCACCC TGACTGGCTG 
CACAAGAAAC TTTTGGAGAA GAATGATGTC TGCAAGCAGA AGAAGATCAG CGAGCTCTTT 
GTCCTCGAGG GCAGGAGACA GGTGGGCATG GCCCAGGTTC CAGAAGGCAC CCAAAGCCTG 
GGTGCTCCTG ATATGGAAGA TTTTGGCCTC ATGAAGCCAT TGCACTTGGC CGTTCCTATT 
GCCACTAAGA GGAAGCGTGT CCTCTGGGAG AGCCAGGAGG AGTCACACGA CCTGGAGCTG 
ACGGTGCCCT GGCAGGAAAT CTTGGGGCAG CCTCCAGCCC TTGGAACCAC CCAGGAAGAG 
TGGCTGGTTT GGCTCCGGTT CCATAAGAAG AAGTGGCAGC TGCAGGCCCG GCAGCGCCTG 
GCTTGCAAGA AGAGGCGGTG TGTGGAGGTG GCAGAGGGCG AACCTCTGCC TGGGGCCATC 
CGAGAAAGGC CTGCCACTGG GTTGGGGGGC TTCCTGCGAA GGACCGCCCG CAGCATCTTG 
GACCTTCCAT GGCAGATTGT GCAGATCAGT GAAACCAGCC AGGCTGGTTT ATTCAGGCTG 
TGGGCTGTCA TCAGCAGTGA CTTGTACTGC ATCAGGCTGA ACATCCCCCG AGTATTCTAT 
GTCAACCAGC AGGTGGCTAA AGCTGAGGAG GGGCCTTCCT ATCGCAAGGT CAGCCGAGTC 
CTTCCTCGCT CCAACATGGT CTACAATCTC TATGAGTACT CCGTGCCTGA GGACATGTAC 
CAGGAACATA TCAATGAAAT CAATACTGAG TTATCAGCCC CAGACATTGA GGGCGTGTAT 
GAGACTCAGG TTCCATTACT ATTCCGCGCC CTAGTGCAGC TGGGCTGTGT CTGTGTGGTC 
AATAAACAGC TGGTGAGGCA CCTTTCAGGC TGGGAAGCAG AAACCTTTGC TCTCGAGCAC 
CTGGAGATGC GTTCTCTGGC TCAGTTCAGC TACCTGGAAC CAGGGAGCAT CCGCCACCTC 
TACCTGTACC ATCATGCACA GGGCCACAAA GCACTCTTCG GCTTGTTCGT GCCCTCCCAG 
CGCAGAGCAT CTGTGTTTGT GTTGGACACT GTGCGAAGCA ACCAGATGCC CAGCCTCAGC 
GCTCTTTACT CAGCCGAGCA CAGCCTTCTG ATGGAGAAAG TGGGCCCGGA GCTCCTGCCT 
CCTCCCAAAC ACACTTTTGA AGTTCGGGCG GAAACTGACA TGAAAACCAT CTGCAGAGCC 
ATCCAGCGCT TCCTGCTGGC CTACAAAGAG GAGCGCCGTG GGCCCACACT CATCGCTGTT 
CAGTCCAACT GGGAGCTAAA GAGGCTGGCT GGTGAGGTTC CTGTCCTAGA GGAATTCCCA 
CTAGTGCCTG TCCACGTGGC AGATAAGATC AGCTATGGAG TCCTGGACTG GCAGCGCCTC 
GGAGCCCGGC GCATGATCCG CCACTACCTC AACCTGGACA CCTCTCTGTC ACAGGCCTTC 
GAGATGAGCA GGTACTTCCA CATCCCCATT GGTAATCTGC CTGAGGACAT CTCCACCTTT 
GGCTCCGACC TCTTCTTTGC CCGCCATCTC CAGCGCCACA GCCACCTGCT CTGGCTGTCG 
CCTACGTCAC GCCCTGACCT GGGTGGCAAG GAGGCTGATG ACAATCGCCT TGTCATGGAA 
TTCGATGACC AGGCCAGCGT GGAGATCAAT TGTCCAGGCT GCTACTCTAC AGTGTGTGTG 
GAGCTGGACA TCCAAAACCT GGCTGTCAAC ACCATCCTGC AGTCCCACCA TATCAATGAC 
ATGGAGGGGG CTGACAGCAT GGGCATCAGC TTTGATGTGA TCCAGCAAGC CTCTCTGGAG 
GACATGATCA CTGGCAACCA GGCCGCCAGC ACTCCAGCCA GCTACGATGA GACAGCCCTC 
TGCTCCAGCA CCTTCAGGAT CCTGAAGAGC ATGGTGGTGG GCTGGGTGAA GGAGATCACA 
CAGTACCGCA ACATCTATGC TGACAACCAG GTGATGCACT TTTACCGCTG GCTCCGGTCC 
CCATCATCAC TGCTCCATGA CCCTGTGCTG CATCGCACAC TCCACAACAT GATGAAGAAG 
CTCTTTCTGC AGCTCATCGC TGAGTTCAAG CGCTTGGGGT CATCTGTCGT CTATGCCAAC 
TTCAACCGCA TCATCCTCTG CACGAAAAAG CGGCGGATCG AGGATGCCAT TGCTTACATG 
GAGTACATCA CCAGCAGCAT CCATTCTAAA GAGATCTTCC ATTCTTTGAC AATATCTTTC 
TCTCGGTGCT GGGAATTTCT TCTCTGGATG GATCCCTCTA ACTATGGTGG AATCAAAGGA 
AATGCTCCAT CTAGTATCCA CTATGGACAG CAAGACTCCC AAAAAGGGGG CAGAGTAGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAAGAG GAGGAGGAAG GCAAACAGGA ACCAGATGTG 
GAGGACATGC TGGAAAACAA CTGGAACATT GTGCAGTTCC TGCCACAGGC AGCCTCCTGC 
CAGAGCTACT TCCTCATGAT TGTTTCAGCA TACATCGTGG CCGTGTACCA CAGCATGAAG 
GAGGAGCTGC GGCGCAGTGC CCCAGGGGGC ACACCCCTGA GATGGCGGGG GCCCAGCCAG 
CTCTCCCAGG ATGCTGCGGC CTCCAGTGCC CTTCCTGCTA TGGTCCCTGC CCTCCTAGGA 
ATGATCACCT TCTCCCAAGA CTACGTGGCC AACGAGCTGA CTCAGAACTT CTTCACCATC 
ACTCAGAAGA TTCAGAAGAA AGTCACAGGC TCCCGGAGCT CCACTGAGCT CTCCAGCATG 
TTTCCTGCCC TTCCAGGTTC TCACTTGCTG CTCAAAAACC CCGCATTGGA GTTCATCAAG 
TACGTGTGCA AGGTTCTGTC TCTGGACACG AACATCACAA ACCAGGTGAA CAAATTGAAC 
CGGGATCTGC TTCGTCTGGT GGATGTTGGC GAGTTCTCGG AGGAGGCACA GTTCCGAGAC 
CCTTGCCGTT CCTACGTGCT CCCTGAGGTC ATCTGCCGCA GCTGTAACTT CTGCCGGGAC 
CTGGACCTGT GTAAAGAGTT CGCCTTCTCG CAGGATGGGG CCGTGCTGCC TCAGTGGCTC 
TGCTCAAACT GTCAGGTTGC CTATGACACC TCAGTCATCG AGATGGCCCT GGTGGAAGCC 
CTGCAGAAGA AACTGATGGC CTTCACTCTG CAGGACCTGA TCTGCCTGAA GTGCCGAGGT 
GTGAAGGAGA CCAACATGCC TGTGTACTGC AGCTGCGCCG GGGGCTTTAC CCTCACCATC 
CACACCAAGG TCTTCATGGA GCAGATTGGA ATCTTCCAGA ACATTGCCCA GCACTATGGC 
ATGTCCTACC TCACAGAGAC CCTGGAGTGG CTGCTGCAGA AGAACCCTCA GCTGGGCCAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265956.1
CDS	16..6858
Translation	MIGEDRISFLLSFRDDGLSSSVSALKRLERSQWTDKMDLRFGFERLKESGEKTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYEPYFYIAARKGCEREVSSFLSKKFQGRIAKIETVPKEDLDLPNHLVGLKRNYIKLSFHTVEDLVRVRKDVSPAVKKNREQGHAGDAYTAMLSSVLQGGSVVIDEEEASKKVTDQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNIRYRGSAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDVEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFDYVQEMKPTIMVTYNGDFFDWPFVEARAAVHGLSMYQEIGFQKDSQGEYKASQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTGDGHVLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPMEHVTNFQEVCDQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAVVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLTAEGPARAFHELSREEQAKYEKRRLSDYCRKAYKKIHVTRVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAMEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRRGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTSVKKPKVTISYPGAMLNIMVKEGFTNDQYQELTEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEIKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPMVRKHFLRKWLKSSSLQDFDIRTILDWEYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVCKQKKISELFVLEGRRQVGMAQVPEGTQSLGAPDMEDFGLMKPLHLAVPIATKRKRVLWESQEESHDLELTVPWQEILGQPPALGTTQEEWLVWLRFHKKKWQLQARQRLACKKRRCVEVAEGEPLPGAIRERPATGLGGFLRRTARSILDLPWQIVQISETSQAGLFRLWAVISSDLYCIRLNIPRVFYVNQQVAKAEEGPSYRKVSRVLPRSNMVYNLYEYSVPEDMYQEHINEINTELSAPDIEGVYETQVPLLFRALVQLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHLYLYHHAQGHKALFGLFVPSQRRASVFVLDTVRSNQMPSLSALYSAEHSLLMEKVGPELLPPPKHTFEVRAETDMKTICRAIQRFLLAYKEERRGPTLIAVQSNWELKRLAGEVPVLEEFPLVPVHVADKISYGVLDWQRLGARRMIRHYLNLDTSLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHSHLLWLSPTSRPDLGGKEADDNRLVMEFDDQASVEINCPGCYSTVCVELDIQNLAVNTILQSHHINDMEGADSMGISFDVIQQASLEDMITGNQAASTPASYDETALCSSTFRILKSMVVGWVKEITQYRNIYADNQVMHFYRWLRSPSSLLHDPVLHRTLHNMMKKLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRIEDAIAYMEYITSSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGNAPSSIHYGQQDSQKGGRVEEEEEEEEEEEEEEGKQEPDVEDMLENNWNIVQFLPQAASCQSYFLMIVSAYIVAVYHSMKEELRRSAPGGTPLRWRGPSQLSQDAAASSALPAMVPALLGMITFSQDYVANELTQNFFTITQKIQKKVTGSRSSTELSSMFPALPGSHLLLKNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCKEFAFSQDGAVLPQWLCSNCQVAYDTSVIEMALVEALQKKLMAFTLQDLICLKCRGVKETNMPVYCSCAGGFTLTIHTKVFMEQIGIFQNIAQHYGMSYLTETLEWLLQKNPQLGH

Target ORF information:

RefSeq Version	XM_022410248.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410248.1

ORF Insert Sequence:

ATGATCGGTG AAGACAGAAT TTCCTTTCTC CTTTCATTCA GGGATGATGG CCTCTCCTCT 
TCTGTCTCAG CACTTAAGCG CCTGGAGCGC AGTCAGTGGA CAGATAAGAT GGATTTGCGG 
TTTGGTTTTG AGAGACTCAA GGAGTCTGGG GAGAAGACGG GCTGGCTTAT CAACATGCAC 
CCTACTGAGA TTTTAGATGA AGACAAACGC TTAGTCAGCG CAGTAGATTA CTACTTTATT 
CAAGATGATG GGAGCAGATT TAAGGTGGCC TTGCCCTACG AGCCGTATTT CTACATTGCA 
GCCAGAAAGG GTTGTGAACG AGAAGTTTCA TCTTTTCTCT CCAAGAAGTT TCAGGGTAGA 
ATTGCTAAAA TAGAGACTGT CCCCAAAGAG GATCTAGACT TGCCAAATCA CTTGGTGGGT 
TTGAAGCGAA ATTACATTAA GCTGTCCTTC CACACTGTGG AGGATCTTGT TAGAGTGAGG 
AAGGATGTCT CCCCTGCTGT GAAGAAGAAC CGGGAGCAGG GTCATGCTGG TGATGCCTAC 
ACAGCTATGC TGTCCAGCGT TCTGCAAGGG GGCAGTGTGG TTATTGATGA AGAGGAAGCC 
TCTAAGAAGG TCACTGACCA GTTGGACAAT ATTGTGGACA TGCGAGAGTA TGATGTGCCC 
TACCACATCC GCCTCTCCAT TGACCTGAAG ATCCATGTGG CTCATTGGTA CAACATCAGA 
TACCGAGGAA GTGCCTTTCC TGTGGAAATC ACCCGCCGAG ATGACCTTGT TGAACGACCT 
GACCCTGTGG TTTTGGCATT TGACATTGAG ACAACCAAAC TGCCTCTCAA GTTTCCTGAT 
GCTGAGACTG ACCAGATTAT GATGATTTCC TACATGATTG ATGGTCAGGG CTACCTCATC 
ACCAACAGGG AGATCGTTTC AGAAGATGTC GAAGATTTCG AGTTCACCCC CAAGCCAGAG 
TATGAAGGAC CTTTTTGTGT CTTTAATGAA CCTGACGAGG TTCATCTAAT CCAGAGATGG 
TTTGACTATG TCCAGGAGAT GAAACCCACC ATCATGGTCA CCTACAATGG GGACTTTTTT 
GACTGGCCGT TTGTGGAGGC CAGAGCAGCA GTCCACGGAC TAAGCATGTA CCAGGAGATC 
GGGTTCCAGA AGGACAGTCA GGGAGAGTAC AAGGCATCCC AGTGCATCCA CATGGACTGT 
CTCAGGTGGG TGAAGAGGGA CAGTTACCTT CCTGTGGGCA GCCACAACCT CAAGGCAGCT 
GCCAAAGCCA AGCTAGGCTA TGACCCAGTG GAGCTGGACC CTGAGGACAT GTGCCGAATG 
GCCACCGAGC AGCCCCAGAC TCTGGCCACG TACTCCGTGT CAGATGCAGT GGCCACGTAC 
TACCTGTACA TGAAATACGT CCACCCCTTT ATATTCGCCC TTTGCACCAT TATCCCCATG 
GAGCCTGATG AGGTGCTGCG GAAGGGCTCT GGGACTCTCT GTGAGGCCTT GCTGATGGTG 
CAGGCCTTCC ACGCCAACAT CATCTTCCCC AATAAGCAGG AGCAGGAGTT CAACAAGCTG 
ACAGGTGATG GCCACGTGCT GGATGCCGAG ACCTATGTGG GTGGCCACGT GGAGGCCCTT 
GAGTCAGGCG TCTTTCGCAG TGACATCCCC TGCCGGTTCA GGATGAATCC TGCTGCCTTT 
GACTTCCTGC TGCAGAGGGT GGAGAAGACC ATGCGCCATG CCATCGAGGA AGAGGAGAAG 
GTGCCCATGG AGCATGTCAC CAACTTCCAA GAGGTGTGTG ATCAGATTAA GACCAAGCTC 
ACCTCCCTGA AAGATGTTCC AAACAGAATA GAGTGTCCCC TTATCTACCA CCTGGATGTG 
GGGGCCATGT ACCCTAACAT CATCCTGACG AACCGCCTGC AGCCCTCTGC CGTGGTGGAT 
GAGGCTACCT GTGCTGCCTG TGACTTCAAT AAGCCTGGGG CCAACTGCCA GAGAAAGATG 
GCCTGGCAGT GGAGGGGCGA GTTCATGCCA GCCAGTCGCA GTGAGTACCA TCGGATCCAG 
CACCAGCTGG AGTCAGAGAA GTTCCCTCCC TTGACTGCAG AGGGTCCAGC CCGGGCCTTT 
CACGAGCTCT CTCGAGAGGA GCAGGCTAAA TATGAGAAGC GGAGGCTGTC AGATTACTGC 
CGGAAGGCAT ACAAGAAGAT ACACGTCACC AGGGTGGAGG AGCGCCTCAC CACTATCTGC 
CAGCGGGAGA ACTCTTTCTA TGTGGACACA GTGCGCGCCT TCCGAGACAG GCGTTATGAA 
TTCAAAGGTC TCCACAAGGT GTGGAAGAAG AAACTCTCTG CAGCCATGGA GGTGGGTGAT 
GCGGCCGAAG TGAAGCGCTG CAAGAACATG GAGGTCCTGT ATGACTCCCT GCAGCTGGCG 
CACAAGTGCA TCCTCAACTC CTTCTATGGC TACGTCATGC GCAGAGGGGC TCGCTGGTAC 
TCCATGGAGA TGGCAGGCAT TGTCTGCTTC ACAGGGGCTA ACATCATCAC CCAGGCACGG 
GAGCTGATTG AGCAGATCGG GAGACCCTTG GAGCTGGACA CAGACGGAAT ATGGTGCGTC 
CTGCCCAACA GCTTTCCTGA AAACTTTGTC ATCAAGACAA CCAGTGTGAA AAAGCCCAAG 
GTGACCATTT CCTACCCTGG GGCCATGTTG AACATCATGG TGAAGGAAGG CTTCACCAAT 
GATCAGTACC AGGAGCTGAC TGAACCATCC TCACTCACCT ATGTCACCCG TTCAGAGAAC 
AGCATCTTTT TCGAGGTTGA CGGACCCTAC CTTGCCATGA TCCTTCCAGC CTCCAAGGAA 
GAAGGCAAGA AACTGAAGAA GAGATATGCT GTCTTCAACG AGGACGGTTC CCTGGCTGAG 
CTGAAGGGCT TCGAGATCAA ACGCCGAGGG GAGCTGCAGC TGATTAAGAT CTTCCAGTCC 
TCAGTGTTCG AGGCTTTCCT CAAGGGCAGC ACCTTAGAAG AAGTGTATGG CTCTGTTGCC 
AAGGTGGCTG ACTACTGGCT GGACGTGCTG TACAGTAAGG CAGCTAACAT GCCCGATTCT 
GAGCTGTTTG AGCTGATCTC TGAGAATCGC TCCATGTCTC GGAAGCTGGA AGATTATGGG 
GAGCAGAAGT CCACGTCTAT CAGCACAGCC AAGCGCCTGG CTGAGTTCCT GGGAGATCAG 
ATGGTGAAGG ATGCAGGGCT GAGCTGCCGT TACATCATCT CTCGGAAGCC CGAGGGCTCT 
CCGGTCACAG AGAGGGCCAT CCCGCTTGCC ATTTTCCAGG CAGAGCCCAT GGTGAGGAAG 
CACTTTCTCC GGAAATGGCT TAAGAGTTCT TCCCTCCAAG ACTTTGATAT TCGCACGATT 
TTGGATTGGG AGTACTACAT TGAGCGGCTA GGGAGTGCCA TACAGAAGAT CATCACGATC 
CCTGCAGCTT TGCAGCAGGT AAAGAACCCA GTACCACGCG TCAAGCACCC TGACTGGCTG 
CACAAGAAAC TTTTGGAGAA GAATGATGTC TGCAAGCAGA AGAAGATCAG CGAGCTCTTT 
GTCCTCGAGG GCAGGAGACA GGTGGGCATG GCCCAGGTTC CAGAAGGCAC CCAAAGCCTG 
GGTGCTCCTG ATATGGAAGA TTTTGGCCTC ATGAAGCCAT TGCACTTGGC CGTTCCTATT 
GCCACTAAGA GGAAGCGTGT CCTCTGGGAG AGCCAGGAGG AGTCACACGA CCTGGAGCTG 
ACGGTGCCCT GGCAGGAAAT CTTGGGGCAG CCTCCAGCCC TTGGAACCAC CCAGGAAGAG 
TGGCTGGTTT GGCTCCGGTT CCATAAGAAG AAGTGGCAGC TGCAGGCCCG GCAGCGCCTG 
GCTTGCAAGA AGAGGCGGTG TGTGGAGGTG GCAGAGGGCG AACCTCTGCC TGGGGCCATC 
CGAGAAAGGC CTGCCACTGG GTTGGGGGGC TTCCTGCGAA GGACCGCCCG CAGCATCTTG 
GACCTTCCAT GGCAGATTGT GCAGATCAGT GAAACCAGCC AGGCTGGTTT ATTCAGGCTG 
TGGGCTGTCA TCAGCAGTGA CTTGTACTGC ATCAGGCTGA ACATCCCCCG AGTATTCTAT 
GTCAACCAGC AGGTGGCTAA AGCTGAGGAG GGGCCTTCCT ATCGCAAGGT CAGCCGAGTC 
CTTCCTCGCT CCAACATGGT CTACAATCTC TATGAGTACT CCGTGCCTGA GGACATGTAC 
CAGGAACATA TCAATGAAAT CAATACTGAG TTATCAGCCC CAGACATTGA GGGCGTGTAT 
GAGACTCAGG TTCCATTACT ATTCCGCGCC CTAGTGCAGC TGGGCTGTGT CTGTGTGGTC 
AATAAACAGC TGGTGAGGCA CCTTTCAGGC TGGGAAGCAG AAACCTTTGC TCTCGAGCAC 
CTGGAGATGC GTTCTCTGGC TCAGTTCAGC TACCTGGAAC CAGGGAGCAT CCGCCACCTC 
TACCTGTACC ATCATGCACA GGGCCACAAA GCACTCTTCG GCTTGTTCGT GCCCTCCCAG 
CGCAGAGCAT CTGTGTTTGT GTTGGACACT GTGCGAAGCA ACCAGATGCC CAGCCTCAGC 
GCTCTTTACT CAGCCGAGCA CAGCCTTCTG ATGGAGAAAG TGGGCCCGGA GCTCCTGCCT 
CCTCCCAAAC ACACTTTTGA AGTTCGGGCG GAAACTGACA TGAAAACCAT CTGCAGAGCC 
ATCCAGCGCT TCCTGCTGGC CTACAAAGAG GAGCGCCGTG GGCCCACACT CATCGCTGTT 
CAGTCCAACT GGGAGCTAAA GAGGCTGGCT GGTGAGGTTC CTGTCCTAGA GGAATTCCCA 
CTAGTGCCTG TCCACGTGGC AGATAAGATC AGCTATGGAG TCCTGGACTG GCAGCGCCTC 
GGAGCCCGGC GCATGATCCG CCACTACCTC AACCTGGACA CCTCTCTGTC ACAGGCCTTC 
GAGATGAGCA GGTACTTCCA CATCCCCATT GGTAATCTGC CTGAGGACAT CTCCACCTTT 
GGCTCCGACC TCTTCTTTGC CCGCCATCTC CAGCGCCACA GCCACCTGCT CTGGCTGTCG 
CCTACGTCAC GCCCTGACCT GGGTGGCAAG GAGGCTGATG ACAATCGCCT TGTCATGGAA 
TTCGATGACC AGGCCAGCGT GGAGATCAAT TGTCCAGGCT GCTACTCTAC AGTGTGTGTG 
GAGCTGGACA TCCAAAACCT GGCTGTCAAC ACCATCCTGC AGTCCCACCA TATCAATGAC 
ATGGAGGGGG CTGACAGCAT GGGCATCAGC TTTGATGTGA TCCAGCAAGC CTCTCTGGAG 
GACATGATCA CTGGCAACCA GGCCGCCAGC ACTCCAGCCA GCTACGATGA GACAGCCCTC 
TGCTCCAGCA CCTTCAGGAT CCTGAAGAGC ATGGTGGTGG GCTGGGTGAA GGAGATCACA 
CAGTACCGCA ACATCTATGC TGACAACCAG GTGATGCACT TTTACCGCTG GCTCCGGTCC 
CCATCATCAC TGCTCCATGA CCCTGTGCTG CATCGCACAC TCCACAACAT GATGAAGAAG 
CTCTTTCTGC AGCTCATCGC TGAGTTCAAG CGCTTGGGGT CATCTGTCGT CTATGCCAAC 
TTCAACCGCA TCATCCTCTG CACGAAAAAG CGGCGGATCG AGGATGCCAT TGCTTACATG 
GAGTACATCA CCAGCAGCAT CCATTCTAAA GAGATCTTCC ATTCTTTGAC AATATCTTTC 
TCTCGGTGCT GGGAATTTCT TCTCTGGATG GATCCCTCTA ACTATGGTGG AATCAAAGGA 
AATGCTCCAT CTAGTATCCA CTATGGACAG CAAGACTCCC AAAAAGGGGG CAGAGTAGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAAGAG GAGGAGGAAG GCAAACAGGA ACCAGATGTG 
GAGGACATGC TGGAAAACAA CTGGAACATT GTGCAGTTCC TGCCACAGGC AGCCTCCTGC 
CAGAGCTACT TCCTCATGAT TGTTTCAGCA TACATCGTGG CCGTGTACCA CAGCATGAAG 
GAGGAGCTGC GGCGCAGTGC CCCAGGGGGC ACACCCCTGA GATGGCGGGG GCCCAGCCAG 
CTCTCCCAGG ATGCTGCGGC CTCCAGTGCC CTTCCTGCTA TGGTCCCTGC CCTCCTAGGA 
ATGATCACCT TCTCCCAAGA CTACGTGGCC AACGAGCTGA CTCAGAACTT CTTCACCATC 
ACTCAGAAGA TTCAGAAGAA AGTCACAGGC TCCCGGAGCT CCACTGAGCT CTCCAGCATG 
TTTCCTGCCC TTCCAGGTTC TCACTTGCTG CTCAAAAACC CCGCATTGGA GTTCATCAAG 
TACGTGTGCA AGGTTCTGTC TCTGGACACG AACATCACAA ACCAGGTGAA CAAATTGAAC 
CGGGATCTGC TTCGTCTGGT GGATGTTGGC GAGTTCTCGG AGGAGGCACA GTTCCGAGAC 
CCTTGCCGTT CCTACGTGCT CCCTGAGGTC ATCTGCCGCA GCTGTAACTT CTGCCGGGAC 
CTGGACCTGT GTAAAGAGTT CGCCTTCTCG CAGGATGGGG CCGTGCTGCC TCAGTGGCTC 
TGCTCAAACT GTCAGGTTGC CTATGACACC TCAGTCATCG AGATGGCCCT GGTGGAAGCC 
CTGCAGAAGA AACTGATGGC CTTCACTCTG CAGGACCTGA TCTGCCTGAA GTGCCGAGGT 
GTGAAGGAGA CCAACATGCC TGTGTACTGC AGCTGCGCCG GGGGCTTTAC CCTCACCATC 
CACACCAAGG TCTTCATGGA GCAGATTGGA ATCTTCCAGA ACATTGCCCA GCACTATGGC 
ATGTCCTACC TCACAGAGAC CCTGGAGTGG CTGCTGCAGA AGAACCCTCA GCTGGGCCAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_022410249.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022265957.1 Canis lupus familiaris DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X5, mRNA.

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CloneID	OCe297422
Clone ID Related Accession (Same CDS sequence)	XM_022410249.1
Accession Version	XM_022410249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	DNA polymerase epsilon catalytic subunit A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGC GGTTTGGTTT TGAGAGACTC AAGGAGTCTG GGGAGAAGAC GGGCTGGCTT 
ATCAACATGC ACCCTACTGA GATTTTAGAT GAAGACAAAC GCTTAGTCAG CGCAGTAGAT 
TACTACTTTA TTCAAGATGA TGGGAGCAGA TTTAAGGTGG CCTTGCCCTA CGAGCCGTAT 
TTCTACATTG CAGCCAGAAA GGGTTGTGAA CGAGAAGTTT CATCTTTTCT CTCCAAGAAG 
TTTCAGGGTA GAATTGCTAA AATAGAGACT GTCCCCAAAG AGGATCTAGA CTTGCCAAAT 
CACTTGGTGG GTTTGAAGCG AAATTACATT AAGCTGTCCT TCCACACTGT GGAGGATCTT 
GTTAGAGTGA GGAAGGATGT CTCCCCTGCT GTGAAGAAGA ACCGGGAGCA GGGTCATGCT 
GGTGATGCCT ACACAGCTAT GCTGTCCAGC GTTCTGCAAG GGGGCAGTGT GGTTATTGAT 
GAAGAGGAAG CCTCTAAGAA GGTCACTGAC CAGTTGGACA ATATTGTGGA CATGCGAGAG 
TATGATGTGC CCTACCACAT CCGCCTCTCC ATTGACCTGA AGATCCATGT GGCTCATTGG 
TACAACATCA GATACCGAGG AAGTGCCTTT CCTGTGGAAA TCACCCGCCG AGATGACCTT 
GTTGAACGAC CTGACCCTGT GGTTTTGGCA TTTGACATTG AGACAACCAA ACTGCCTCTC 
AAGTTTCCTG ATGCTGAGAC TGACCAGATT ATGATGATTT CCTACATGAT TGATGGTCAG 
GGCTACCTCA TCACCAACAG GGAGATCGTT TCAGAAGATG TCGAAGATTT CGAGTTCACC 
CCCAAGCCAG AGTATGAAGG ACCTTTTTGT GTCTTTAATG AACCTGACGA GGTTCATCTA 
ATCCAGAGAT GGTTTGACTA TGTCCAGGAG ATGAAACCCA CCATCATGGT CACCTACAAT 
GGGGACTTTT TTGACTGGCC GTTTGTGGAG GCCAGAGCAG CAGTCCACGG ACTAAGCATG 
TACCAGGAGA TCGGGTTCCA GAAGGACAGT CAGGGAGAGT ACAAGGCATC CCAGTGCATC 
CACATGGACT GTCTCAGGTG GGTGAAGAGG GACAGTTACC TTCCTGTGGG CAGCCACAAC 
CTCAAGGCAG CTGCCAAAGC CAAGCTAGGC TATGACCCAG TGGAGCTGGA CCCTGAGGAC 
ATGTGCCGAA TGGCCACCGA GCAGCCCCAG ACTCTGGCCA CGTACTCCGT GTCAGATGCA 
GTGGCCACGT ACTACCTGTA CATGAAATAC GTCCACCCCT TTATATTCGC CCTTTGCACC 
ATTATCCCCA TGGAGCCTGA TGAGGTGCTG CGGAAGGGCT CTGGGACTCT CTGTGAGGCC 
TTGCTGATGG TGCAGGCCTT CCACGCCAAC ATCATCTTCC CCAATAAGCA GGAGCAGGAG 
TTCAACAAGC TGACAGGTGA TGGCCACGTG CTGGATGCCG AGACCTATGT GGGTGGCCAC 
GTGGAGGCCC TTGAGTCAGG CGTCTTTCGC AGTGACATCC CCTGCCGGTT CAGGATGAAT 
CCTGCTGCCT TTGACTTCCT GCTGCAGAGG GTGGAGAAGA CCATGCGCCA TGCCATCGAG 
GAAGAGGAGA AGGTGCCCAT GGAGCATGTC ACCAACTTCC AAGAGGTGTG TGATCAGATT 
AAGACCAAGC TCACCTCCCT GAAAGATGTT CCAAACAGAA TAGAGTGTCC CCTTATCTAC 
CACCTGGATG TGGGGGCCAT GTACCCTAAC ATCATCCTGA CGAACCGCCT GCAGCCCTCT 
GCCGTGGTGG ATGAGGCTAC CTGTGCTGCC TGTGACTTCA ATAAGCCTGG GGCCAACTGC 
CAGAGAAAGA TGGCCTGGCA GTGGAGGGGC GAGTTCATGC CAGCCAGTCG CAGTGAGTAC 
CATCGGATCC AGCACCAGCT GGAGTCAGAG AAGTTCCCTC CCTTGACTGC AGAGGGTCCA 
GCCCGGGCCT TTCACGAGCT CTCTCGAGAG GAGCAGGCTA AATATGAGAA GCGGAGGCTG 
TCAGATTACT GCCGGAAGGC ATACAAGAAG ATACACGTCA CCAGGGTGGA GGAGCGCCTC 
ACCACTATCT GCCAGCGGGA GAACTCTTTC TATGTGGACA CAGTGCGCGC CTTCCGAGAC 
AGGCGTTATG AATTCAAAGG TCTCCACAAG GTGTGGAAGA AGAAACTCTC TGCAGCCATG 
GAGGTGGGTG ATGCGGCCGA AGTGAAGCGC TGCAAGAACA TGGAGGTCCT GTATGACTCC 
CTGCAGCTGG CGCACAAGTG CATCCTCAAC TCCTTCTATG GCTACGTCAT GCGCAGAGGG 
GCTCGCTGGT ACTCCATGGA GATGGCAGGC ATTGTCTGCT TCACAGGGGC TAACATCATC 
ACCCAGGCAC GGGAGCTGAT TGAGCAGATC GGGAGACCCT TGGAGCTGGA CACAGACGGA 
ATATGGTGCG TCCTGCCCAA CAGCTTTCCT GAAAACTTTG TCATCAAGAC AACCAGTGTG 
AAAAAGCCCA AGGTGACCAT TTCCTACCCT GGGGCCATGT TGAACATCAT GGTGAAGGAA 
GGCTTCACCA ATGATCAGTA CCAGGAGCTG ACTGAACCAT CCTCACTCAC CTATGTCACC 
CGTTCAGAGA ACAGCATCTT TTTCGAGGTT GACGGACCCT ACCTTGCCAT GATCCTTCCA 
GCCTCCAAGG AAGAAGGCAA GAAACTGAAG AAGAGATATG CTGTCTTCAA CGAGGACGGT 
TCCCTGGCTG AGCTGAAGGG CTTCGAGATC AAACGCCGAG GGGAGCTGCA GCTGATTAAG 
ATCTTCCAGT CCTCAGTGTT CGAGGCTTTC CTCAAGGGCA GCACCTTAGA AGAAGTGTAT 
GGCTCTGTTG CCAAGGTGGC TGACTACTGG CTGGACGTGC TGTACAGTAA GGCAGCTAAC 
ATGCCCGATT CTGAGCTGTT TGAGCTGATC TCTGAGAATC GCTCCATGTC TCGGAAGCTG 
GAAGATTATG GGGAGCAGAA GTCCACGTCT ATCAGCACAG CCAAGCGCCT GGCTGAGTTC 
CTGGGAGATC AGATGGTGAA GGATGCAGGG CTGAGCTGCC GTTACATCAT CTCTCGGAAG 
CCCGAGGGCT CTCCGGTCAC AGAGAGGGCC ATCCCGCTTG CCATTTTCCA GGCAGAGCCC 
ATGGTGAGGA AGCACTTTCT CCGGAAATGG CTTAAGAGTT CTTCCCTCCA AGACTTTGAT 
ATTCGCACGA TTTTGGATTG GGAGTACTAC ATTGAGCGGC TAGGGAGTGC CATACAGAAG 
ATCATCACGA TCCCTGCAGC TTTGCAGCAG GTAAAGAACC CAGTACCACG CGTCAAGCAC 
CCTGACTGGC TGCACAAGAA ACTTTTGGAG AAGAATGATG TCTGCAAGCA GAAGAAGATC 
AGCGAGCTCT TTGTCCTCGA GGGCAGGAGA CAGGTGGGCA TGGCCCAGGT TCCAGAAGGC 
ACCCAAAGCC TGGGTGCTCC TGATATGGAA GATTTTGGCC TCATGAAGCC ATTGCACTTG 
GCCGTTCCTA TTGCCACTAA GAGGAAGCGT GTCCTCTGGG AGAGCCAGGA GGAGTCACAC 
GACCTGGAGC TGACGGTGCC CTGGCAGGAA ATCTTGGGGC AGCCTCCAGC CCTTGGAACC 
ACCCAGGAAG AGTGGCTGGT TTGGCTCCGG TTCCATAAGA AGAAGTGGCA GCTGCAGGCC 
CGGCAGCGCC TGGCTTGCAA GAAGAGGCGG TGTGTGGAGG TGGCAGAGGG CGAACCTCTG 
CCTGGGGCCA TCCGAGAAAG GCCTGCCACT GGGTTGGGGG GCTTCCTGCG AAGGACCGCC 
CGCAGCATCT TGGACCTTCC ATGGCAGATT GTGCAGATCA GTGAAACCAG CCAGGCTGGT 
TTATTCAGGC TGTGGGCTGT CATCAGCAGT GACTTGTACT GCATCAGGCT GAACATCCCC 
CGAGTATTCT ATGTCAACCA GCAGGTGGCT AAAGCTGAGG AGGGGCCTTC CTATCGCAAG 
GTCAGCCGAG TCCTTCCTCG CTCCAACATG GTCTACAATC TCTATGAGTA CTCCGTGCCT 
GAGGACATGT ACCAGGAACA TATCAATGAA ATCAATACTG AGTTATCAGC CCCAGACATT 
GAGGGCGTGT ATGAGACTCA GGTTCCATTA CTATTCCGCG CCCTAGTGCA GCTGGGCTGT 
GTCTGTGTGG TCAATAAACA GCTGGTGAGG CACCTTTCAG GCTGGGAAGC AGAAACCTTT 
GCTCTCGAGC ACCTGGAGAT GCGTTCTCTG GCTCAGTTCA GCTACCTGGA ACCAGGGAGC 
ATCCGCCACC TCTACCTGTA CCATCATGCA CAGGGCCACA AAGCACTCTT CGGCTTGTTC 
GTGCCCTCCC AGCGCAGAGC ATCTGTGTTT GTGTTGGACA CTGTGCGAAG CAACCAGATG 
CCCAGCCTCA GCGCTCTTTA CTCAGCCGAG CACAGCCTTC TGATGGAGAA AGTGGGCCCG 
GAGCTCCTGC CTCCTCCCAA ACACACTTTT GAAGTTCGGG CGGAAACTGA CATGAAAACC 
ATCTGCAGAG CCATCCAGCG CTTCCTGCTG GCCTACAAAG AGGAGCGCCG TGGGCCCACA 
CTCATCGCTG TTCAGTCCAA CTGGGAGCTA AAGAGGCTGG CTGGTGAGGT TCCTGTCCTA 
GAGGAATTCC CACTAGTGCC TGTCCACGTG GCAGATAAGA TCAGCTATGG AGTCCTGGAC 
TGGCAGCGCC TCGGAGCCCG GCGCATGATC CGCCACTACC TCAACCTGGA CACCTCTCTG 
TCACAGGCCT TCGAGATGAG CAGGTACTTC CACATCCCCA TTGGTAATCT GCCTGAGGAC 
ATCTCCACCT TTGGCTCCGA CCTCTTCTTT GCCCGCCATC TCCAGCGCCA CAGCCACCTG 
CTCTGGCTGT CGCCTACGTC ACGCCCTGAC CTGGGTGGCA AGGAGGCTGA TGACAATCGC 
CTTGTCATGG AATTCGATGA CCAGGCCAGC GTGGAGATCA ATTGTCCAGG CTGCTACTCT 
ACAGTGTGTG TGGAGCTGGA CATCCAAAAC CTGGCTGTCA ACACCATCCT GCAGTCCCAC 
CATATCAATG ACATGGAGGG GGCTGACAGC ATGGGCATCA GCTTTGATGT GATCCAGCAA 
GCCTCTCTGG AGGACATGAT CACTGGCAAC CAGGCCGCCA GCACTCCAGC CAGCTACGAT 
GAGACAGCCC TCTGCTCCAG CACCTTCAGG ATCCTGAAGA GCATGGTGGT GGGCTGGGTG 
AAGGAGATCA CACAGTACCG CAACATCTAT GCTGACAACC AGGTGATGCA CTTTTACCGC 
TGGCTCCGGT CCCCATCATC ACTGCTCCAT GACCCTGTGC TGCATCGCAC ACTCCACAAC 
ATGATGAAGA AGCTCTTTCT GCACATCCAT TCTAAAGAGA TCTTCCATTC TTTGACAATA 
TCTTTCTCTC GGTGCTGGGA ATTTCTTCTC TGGATGGATC CCTCTAACTA TGGTGGAATC 
AAAGGAAATG CTCCATCTAG TATCCACTAT GGACAGCAAG ACTCCCAAAA AGGGGGCAGA 
GTAGAGGAGG AGGAGGAGGA GGAGGAGGAG GAAGAGGAGG AGGAAGGCAA ACAGGAACCA 
GATGTGGAGG ACATGCTGGA AAACAACTGG AACATTGTGC AGTTCCTGCC ACAGGCAGCC 
TCCTGCCAGA GCTACTTCCT CATGATTGTT TCAGCATACA TCGTGGCCGT GTACCACAGC 
ATGAAGGAGG AGCTGCGGCG CAGTGCCCCA GGGGGCACAC CCCTGAGATG GCGGGGGCCC 
AGCCAGCTCT CCCAGGATGC TGCGGCCTCC AGTGCCCTTC CTGCTATGGT CCCTGCCCTC 
CTAGGAATGA TCACCTTCTC CCAAGACTAC GTGGCCAACG AGCTGACTCA GAACTTCTTC 
ACCATCACTC AGAAGATTCA GAAGAAAGTC ACAGGCTCCC GGAGCTCCAC TGAGCTCTCC 
AGCATGTTTC CTGCCCTTCC AGGTTCTCAC TTGCTGCTCA AAAACCCCGC ATTGGAGTTC 
ATCAAGTACG TGTGCAAGGT TCTGTCTCTG GACACGAACA TCACAAACCA GGTGAACAAA 
TTGAACCGGG ATCTGCTTCG TCTGGTGGAT GTTGGCGAGT TCTCGGAGGA GGCACAGTTC 
CGAGACCCTT GCCGTTCCTA CGTGCTCCCT GAGGTCATCT GCCGCAGCTG TAACTTCTGC 
CGGGACCTGG ACCTGTGTAA AGAGTTCGCC TTCTCGCAGG ATGGGGCCGT GCTGCCTCAG 
TGGCTCTGCT CAAACTGTCA GGTTGCCTAT GACACCTCAG TCATCGAGAT GGCCCTGGTG 
GAAGCCCTGC AGAAGAAACT GATGGCCTTC ACTCTGCAGG ACCTGGCTGG GGAGACTCCT 
GACCAGGACT GCTCCTCATT GCAGATCTGC CTGAAGTGCC GAGGTGTGAA GGAGACCAAC 
ATGCCTGTGT ACTGCAGCTG CGCCGGGGGC TTTACCCTCA CCATCCACAC CAAGGTCTTC 
ATGGAGCAGA TTGGAATCTT CCAGAACATT GCCCAGCACT ATGGCATGTC CTACCTCACA 
GAGACCCTGG AGTGGCTGCT GCAGAAGAAC CCTCAGCTGG GCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022265957.1
CDS	85..6732
Translation	MDLRFGFERLKESGEKTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYEPYFYIAARKGCEREVSSFLSKKFQGRIAKIETVPKEDLDLPNHLVGLKRNYIKLSFHTVEDLVRVRKDVSPAVKKNREQGHAGDAYTAMLSSVLQGGSVVIDEEEASKKVTDQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNIRYRGSAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDVEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFDYVQEMKPTIMVTYNGDFFDWPFVEARAAVHGLSMYQEIGFQKDSQGEYKASQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTGDGHVLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPMEHVTNFQEVCDQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAVVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLTAEGPARAFHELSREEQAKYEKRRLSDYCRKAYKKIHVTRVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAMEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRRGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTSVKKPKVTISYPGAMLNIMVKEGFTNDQYQELTEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEIKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPMVRKHFLRKWLKSSSLQDFDIRTILDWEYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVCKQKKISELFVLEGRRQVGMAQVPEGTQSLGAPDMEDFGLMKPLHLAVPIATKRKRVLWESQEESHDLELTVPWQEILGQPPALGTTQEEWLVWLRFHKKKWQLQARQRLACKKRRCVEVAEGEPLPGAIRERPATGLGGFLRRTARSILDLPWQIVQISETSQAGLFRLWAVISSDLYCIRLNIPRVFYVNQQVAKAEEGPSYRKVSRVLPRSNMVYNLYEYSVPEDMYQEHINEINTELSAPDIEGVYETQVPLLFRALVQLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHLYLYHHAQGHKALFGLFVPSQRRASVFVLDTVRSNQMPSLSALYSAEHSLLMEKVGPELLPPPKHTFEVRAETDMKTICRAIQRFLLAYKEERRGPTLIAVQSNWELKRLAGEVPVLEEFPLVPVHVADKISYGVLDWQRLGARRMIRHYLNLDTSLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHSHLLWLSPTSRPDLGGKEADDNRLVMEFDDQASVEINCPGCYSTVCVELDIQNLAVNTILQSHHINDMEGADSMGISFDVIQQASLEDMITGNQAASTPASYDETALCSSTFRILKSMVVGWVKEITQYRNIYADNQVMHFYRWLRSPSSLLHDPVLHRTLHNMMKKLFLHIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGNAPSSIHYGQQDSQKGGRVEEEEEEEEEEEEEEGKQEPDVEDMLENNWNIVQFLPQAASCQSYFLMIVSAYIVAVYHSMKEELRRSAPGGTPLRWRGPSQLSQDAAASSALPAMVPALLGMITFSQDYVANELTQNFFTITQKIQKKVTGSRSSTELSSMFPALPGSHLLLKNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCKEFAFSQDGAVLPQWLCSNCQVAYDTSVIEMALVEALQKKLMAFTLQDLAGETPDQDCSSLQICLKCRGVKETNMPVYCSCAGGFTLTIHTKVFMEQIGIFQNIAQHYGMSYLTETLEWLLQKNPQLGH

Target ORF information:

RefSeq Version	XM_022410249.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022410249.1

ORF Insert Sequence:

ATGGATTTGC GGTTTGGTTT TGAGAGACTC AAGGAGTCTG GGGAGAAGAC GGGCTGGCTT 
ATCAACATGC ACCCTACTGA GATTTTAGAT GAAGACAAAC GCTTAGTCAG CGCAGTAGAT 
TACTACTTTA TTCAAGATGA TGGGAGCAGA TTTAAGGTGG CCTTGCCCTA CGAGCCGTAT 
TTCTACATTG CAGCCAGAAA GGGTTGTGAA CGAGAAGTTT CATCTTTTCT CTCCAAGAAG 
TTTCAGGGTA GAATTGCTAA AATAGAGACT GTCCCCAAAG AGGATCTAGA CTTGCCAAAT 
CACTTGGTGG GTTTGAAGCG AAATTACATT AAGCTGTCCT TCCACACTGT GGAGGATCTT 
GTTAGAGTGA GGAAGGATGT CTCCCCTGCT GTGAAGAAGA ACCGGGAGCA GGGTCATGCT 
GGTGATGCCT ACACAGCTAT GCTGTCCAGC GTTCTGCAAG GGGGCAGTGT GGTTATTGAT 
GAAGAGGAAG CCTCTAAGAA GGTCACTGAC CAGTTGGACA ATATTGTGGA CATGCGAGAG 
TATGATGTGC CCTACCACAT CCGCCTCTCC ATTGACCTGA AGATCCATGT GGCTCATTGG 
TACAACATCA GATACCGAGG AAGTGCCTTT CCTGTGGAAA TCACCCGCCG AGATGACCTT 
GTTGAACGAC CTGACCCTGT GGTTTTGGCA TTTGACATTG AGACAACCAA ACTGCCTCTC 
AAGTTTCCTG ATGCTGAGAC TGACCAGATT ATGATGATTT CCTACATGAT TGATGGTCAG 
GGCTACCTCA TCACCAACAG GGAGATCGTT TCAGAAGATG TCGAAGATTT CGAGTTCACC 
CCCAAGCCAG AGTATGAAGG ACCTTTTTGT GTCTTTAATG AACCTGACGA GGTTCATCTA 
ATCCAGAGAT GGTTTGACTA TGTCCAGGAG ATGAAACCCA CCATCATGGT CACCTACAAT 
GGGGACTTTT TTGACTGGCC GTTTGTGGAG GCCAGAGCAG CAGTCCACGG ACTAAGCATG 
TACCAGGAGA TCGGGTTCCA GAAGGACAGT CAGGGAGAGT ACAAGGCATC CCAGTGCATC 
CACATGGACT GTCTCAGGTG GGTGAAGAGG GACAGTTACC TTCCTGTGGG CAGCCACAAC 
CTCAAGGCAG CTGCCAAAGC CAAGCTAGGC TATGACCCAG TGGAGCTGGA CCCTGAGGAC 
ATGTGCCGAA TGGCCACCGA GCAGCCCCAG ACTCTGGCCA CGTACTCCGT GTCAGATGCA 
GTGGCCACGT ACTACCTGTA CATGAAATAC GTCCACCCCT TTATATTCGC CCTTTGCACC 
ATTATCCCCA TGGAGCCTGA TGAGGTGCTG CGGAAGGGCT CTGGGACTCT CTGTGAGGCC 
TTGCTGATGG TGCAGGCCTT CCACGCCAAC ATCATCTTCC CCAATAAGCA GGAGCAGGAG 
TTCAACAAGC TGACAGGTGA TGGCCACGTG CTGGATGCCG AGACCTATGT GGGTGGCCAC 
GTGGAGGCCC TTGAGTCAGG CGTCTTTCGC AGTGACATCC CCTGCCGGTT CAGGATGAAT 
CCTGCTGCCT TTGACTTCCT GCTGCAGAGG GTGGAGAAGA CCATGCGCCA TGCCATCGAG 
GAAGAGGAGA AGGTGCCCAT GGAGCATGTC ACCAACTTCC AAGAGGTGTG TGATCAGATT 
AAGACCAAGC TCACCTCCCT GAAAGATGTT CCAAACAGAA TAGAGTGTCC CCTTATCTAC 
CACCTGGATG TGGGGGCCAT GTACCCTAAC ATCATCCTGA CGAACCGCCT GCAGCCCTCT 
GCCGTGGTGG ATGAGGCTAC CTGTGCTGCC TGTGACTTCA ATAAGCCTGG GGCCAACTGC 
CAGAGAAAGA TGGCCTGGCA GTGGAGGGGC GAGTTCATGC CAGCCAGTCG CAGTGAGTAC 
CATCGGATCC AGCACCAGCT GGAGTCAGAG AAGTTCCCTC CCTTGACTGC AGAGGGTCCA 
GCCCGGGCCT TTCACGAGCT CTCTCGAGAG GAGCAGGCTA AATATGAGAA GCGGAGGCTG 
TCAGATTACT GCCGGAAGGC ATACAAGAAG ATACACGTCA CCAGGGTGGA GGAGCGCCTC 
ACCACTATCT GCCAGCGGGA GAACTCTTTC TATGTGGACA CAGTGCGCGC CTTCCGAGAC 
AGGCGTTATG AATTCAAAGG TCTCCACAAG GTGTGGAAGA AGAAACTCTC TGCAGCCATG 
GAGGTGGGTG ATGCGGCCGA AGTGAAGCGC TGCAAGAACA TGGAGGTCCT GTATGACTCC 
CTGCAGCTGG CGCACAAGTG CATCCTCAAC TCCTTCTATG GCTACGTCAT GCGCAGAGGG 
GCTCGCTGGT ACTCCATGGA GATGGCAGGC ATTGTCTGCT TCACAGGGGC TAACATCATC 
ACCCAGGCAC GGGAGCTGAT TGAGCAGATC GGGAGACCCT TGGAGCTGGA CACAGACGGA 
ATATGGTGCG TCCTGCCCAA CAGCTTTCCT GAAAACTTTG TCATCAAGAC AACCAGTGTG 
AAAAAGCCCA AGGTGACCAT TTCCTACCCT GGGGCCATGT TGAACATCAT GGTGAAGGAA 
GGCTTCACCA ATGATCAGTA CCAGGAGCTG ACTGAACCAT CCTCACTCAC CTATGTCACC 
CGTTCAGAGA ACAGCATCTT TTTCGAGGTT GACGGACCCT ACCTTGCCAT GATCCTTCCA 
GCCTCCAAGG AAGAAGGCAA GAAACTGAAG AAGAGATATG CTGTCTTCAA CGAGGACGGT 
TCCCTGGCTG AGCTGAAGGG CTTCGAGATC AAACGCCGAG GGGAGCTGCA GCTGATTAAG 
ATCTTCCAGT CCTCAGTGTT CGAGGCTTTC CTCAAGGGCA GCACCTTAGA AGAAGTGTAT 
GGCTCTGTTG CCAAGGTGGC TGACTACTGG CTGGACGTGC TGTACAGTAA GGCAGCTAAC 
ATGCCCGATT CTGAGCTGTT TGAGCTGATC TCTGAGAATC GCTCCATGTC TCGGAAGCTG 
GAAGATTATG GGGAGCAGAA GTCCACGTCT ATCAGCACAG CCAAGCGCCT GGCTGAGTTC 
CTGGGAGATC AGATGGTGAA GGATGCAGGG CTGAGCTGCC GTTACATCAT CTCTCGGAAG 
CCCGAGGGCT CTCCGGTCAC AGAGAGGGCC ATCCCGCTTG CCATTTTCCA GGCAGAGCCC 
ATGGTGAGGA AGCACTTTCT CCGGAAATGG CTTAAGAGTT CTTCCCTCCA AGACTTTGAT 
ATTCGCACGA TTTTGGATTG GGAGTACTAC ATTGAGCGGC TAGGGAGTGC CATACAGAAG 
ATCATCACGA TCCCTGCAGC TTTGCAGCAG GTAAAGAACC CAGTACCACG CGTCAAGCAC 
CCTGACTGGC TGCACAAGAA ACTTTTGGAG AAGAATGATG TCTGCAAGCA GAAGAAGATC 
AGCGAGCTCT TTGTCCTCGA GGGCAGGAGA CAGGTGGGCA TGGCCCAGGT TCCAGAAGGC 
ACCCAAAGCC TGGGTGCTCC TGATATGGAA GATTTTGGCC TCATGAAGCC ATTGCACTTG 
GCCGTTCCTA TTGCCACTAA GAGGAAGCGT GTCCTCTGGG AGAGCCAGGA GGAGTCACAC 
GACCTGGAGC TGACGGTGCC CTGGCAGGAA ATCTTGGGGC AGCCTCCAGC CCTTGGAACC 
ACCCAGGAAG AGTGGCTGGT TTGGCTCCGG TTCCATAAGA AGAAGTGGCA GCTGCAGGCC 
CGGCAGCGCC TGGCTTGCAA GAAGAGGCGG TGTGTGGAGG TGGCAGAGGG CGAACCTCTG 
CCTGGGGCCA TCCGAGAAAG GCCTGCCACT GGGTTGGGGG GCTTCCTGCG AAGGACCGCC 
CGCAGCATCT TGGACCTTCC ATGGCAGATT GTGCAGATCA GTGAAACCAG CCAGGCTGGT 
TTATTCAGGC TGTGGGCTGT CATCAGCAGT GACTTGTACT GCATCAGGCT GAACATCCCC 
CGAGTATTCT ATGTCAACCA GCAGGTGGCT AAAGCTGAGG AGGGGCCTTC CTATCGCAAG 
GTCAGCCGAG TCCTTCCTCG CTCCAACATG GTCTACAATC TCTATGAGTA CTCCGTGCCT 
GAGGACATGT ACCAGGAACA TATCAATGAA ATCAATACTG AGTTATCAGC CCCAGACATT 
GAGGGCGTGT ATGAGACTCA GGTTCCATTA CTATTCCGCG CCCTAGTGCA GCTGGGCTGT 
GTCTGTGTGG TCAATAAACA GCTGGTGAGG CACCTTTCAG GCTGGGAAGC AGAAACCTTT 
GCTCTCGAGC ACCTGGAGAT GCGTTCTCTG GCTCAGTTCA GCTACCTGGA ACCAGGGAGC 
ATCCGCCACC TCTACCTGTA CCATCATGCA CAGGGCCACA AAGCACTCTT CGGCTTGTTC 
GTGCCCTCCC AGCGCAGAGC ATCTGTGTTT GTGTTGGACA CTGTGCGAAG CAACCAGATG 
CCCAGCCTCA GCGCTCTTTA CTCAGCCGAG CACAGCCTTC TGATGGAGAA AGTGGGCCCG 
GAGCTCCTGC CTCCTCCCAA ACACACTTTT GAAGTTCGGG CGGAAACTGA CATGAAAACC 
ATCTGCAGAG CCATCCAGCG CTTCCTGCTG GCCTACAAAG AGGAGCGCCG TGGGCCCACA 
CTCATCGCTG TTCAGTCCAA CTGGGAGCTA AAGAGGCTGG CTGGTGAGGT TCCTGTCCTA 
GAGGAATTCC CACTAGTGCC TGTCCACGTG GCAGATAAGA TCAGCTATGG AGTCCTGGAC 
TGGCAGCGCC TCGGAGCCCG GCGCATGATC CGCCACTACC TCAACCTGGA CACCTCTCTG 
TCACAGGCCT TCGAGATGAG CAGGTACTTC CACATCCCCA TTGGTAATCT GCCTGAGGAC 
ATCTCCACCT TTGGCTCCGA CCTCTTCTTT GCCCGCCATC TCCAGCGCCA CAGCCACCTG 
CTCTGGCTGT CGCCTACGTC ACGCCCTGAC CTGGGTGGCA AGGAGGCTGA TGACAATCGC 
CTTGTCATGG AATTCGATGA CCAGGCCAGC GTGGAGATCA ATTGTCCAGG CTGCTACTCT 
ACAGTGTGTG TGGAGCTGGA CATCCAAAAC CTGGCTGTCA ACACCATCCT GCAGTCCCAC 
CATATCAATG ACATGGAGGG GGCTGACAGC ATGGGCATCA GCTTTGATGT GATCCAGCAA 
GCCTCTCTGG AGGACATGAT CACTGGCAAC CAGGCCGCCA GCACTCCAGC CAGCTACGAT 
GAGACAGCCC TCTGCTCCAG CACCTTCAGG ATCCTGAAGA GCATGGTGGT GGGCTGGGTG 
AAGGAGATCA CACAGTACCG CAACATCTAT GCTGACAACC AGGTGATGCA CTTTTACCGC 
TGGCTCCGGT CCCCATCATC ACTGCTCCAT GACCCTGTGC TGCATCGCAC ACTCCACAAC 
ATGATGAAGA AGCTCTTTCT GCACATCCAT TCTAAAGAGA TCTTCCATTC TTTGACAATA 
TCTTTCTCTC GGTGCTGGGA ATTTCTTCTC TGGATGGATC CCTCTAACTA TGGTGGAATC 
AAAGGAAATG CTCCATCTAG TATCCACTAT GGACAGCAAG ACTCCCAAAA AGGGGGCAGA 
GTAGAGGAGG AGGAGGAGGA GGAGGAGGAG GAAGAGGAGG AGGAAGGCAA ACAGGAACCA 
GATGTGGAGG ACATGCTGGA AAACAACTGG AACATTGTGC AGTTCCTGCC ACAGGCAGCC 
TCCTGCCAGA GCTACTTCCT CATGATTGTT TCAGCATACA TCGTGGCCGT GTACCACAGC 
ATGAAGGAGG AGCTGCGGCG CAGTGCCCCA GGGGGCACAC CCCTGAGATG GCGGGGGCCC 
AGCCAGCTCT CCCAGGATGC TGCGGCCTCC AGTGCCCTTC CTGCTATGGT CCCTGCCCTC 
CTAGGAATGA TCACCTTCTC CCAAGACTAC GTGGCCAACG AGCTGACTCA GAACTTCTTC 
ACCATCACTC AGAAGATTCA GAAGAAAGTC ACAGGCTCCC GGAGCTCCAC TGAGCTCTCC 
AGCATGTTTC CTGCCCTTCC AGGTTCTCAC TTGCTGCTCA AAAACCCCGC ATTGGAGTTC 
ATCAAGTACG TGTGCAAGGT TCTGTCTCTG GACACGAACA TCACAAACCA GGTGAACAAA 
TTGAACCGGG ATCTGCTTCG TCTGGTGGAT GTTGGCGAGT TCTCGGAGGA GGCACAGTTC 
CGAGACCCTT GCCGTTCCTA CGTGCTCCCT GAGGTCATCT GCCGCAGCTG TAACTTCTGC 
CGGGACCTGG ACCTGTGTAA AGAGTTCGCC TTCTCGCAGG ATGGGGCCGT GCTGCCTCAG 
TGGCTCTGCT CAAACTGTCA GGTTGCCTAT GACACCTCAG TCATCGAGAT GGCCCTGGTG 
GAAGCCCTGC AGAAGAAACT GATGGCCTTC ACTCTGCAGG ACCTGGCTGG GGAGACTCCT 
GACCAGGACT GCTCCTCATT GCAGATCTGC CTGAAGTGCC GAGGTGTGAA GGAGACCAAC 
ATGCCTGTGT ACTGCAGCTG CGCCGGGGGC TTTACCCTCA CCATCCACAC CAAGGTCTTC 
ATGGAGCAGA TTGGAATCTT CCAGAACATT GCCCAGCACT ATGGCATGTC CTACCTCACA 
GAGACCCTGG AGTGGCTGCT GCAGAAGAAC CCTCAGCTGG GCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_014108071.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013963546.1 Canis lupus familiaris polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.

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CloneID	OCe43391
Clone ID Related Accession (Same CDS sequence)	XM_014108071.1
Accession Version	XM_014108071.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	DNA polymerase epsilon catalytic subunit A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTGC GGTTTGGTTT TGAGAGACTC AAGGAGTCTG GGGAGAAGAC GGGCTGGCTT 
ATCAACATGC ACCCTACTGA GATTTTAGAT GAAGACAAAC GCTTAGTCAG CGCAGTAGAT 
TACTACTTTA TTCAAGATGA TGGGAGCAGA TTTAAGGTGG CCTTGCCCTA CGAGCCGTAT 
TTCTACATTG CAGCCAGAAA GGGTTGTGAA CGAGAAGTTT CATCTTTTCT CTCCAAGAAG 
TTTCAGGGTA GAATTGCTAA AATAGAGACT GTCCCCAAAG AGGATCTAGA CTTGCCAAAT 
CACTTGGTGG GTTTGAAGCG AAATTACATT AAGCTGTCCT TCCACACTGT GGAGGATCTT 
GTTAGAGTGA GGAAGGATGT CTCCCCTGCT GTGAAGAAGA ACCGGGAGCA GGGTCATGCT 
GGTGATGCCT ACACAGCTAT GCTGTCCAGC GTTCTGCAAG GGGGCAGTGT GGTTATTGAT 
GAAGAGGAAG CCTCTAAGAA GGTCACTGAC CAGTTGGACA ATATTGTGGA CATGCGAGAG 
TATGATGTGC CCTACCACAT CCGCCTCTCC ATTGACCTGA AGATCCATGT GGCTCATTGG 
TACAACATCA GATACCGAGG AAGTGCCTTT CCTGTGGAAA TCACCCGCCG AGATGACCTT 
GTTGAACGAC CTGACCCTGT GGTTTTGGCA TTTGACATTG AGACAACCAA ACTGCCTCTC 
AAGTTTCCTG ATGCTGAGAC TGACCAGATT ATGATGATTT CCTACATGAT TGATGGTCAG 
GGCTACCTCA TCACCAACAG GGAGATCGTT TCAGAAGATG TCGAAGATTT CGAGTTCACC 
CCCAAGCCAG AGTATGAAGG ACCTTTTTGT GTCTTTAATG AACCTGACGA GGTTCATCTA 
ATCCAGAGAT GGTTTGACTA TGTCCAGGAG ATGAAACCCA CCATCATGGT CACCTACAAT 
GGGGACTTTT TTGACTGGCC GTTTGTGGAG GCCAGAGCAG CAGTCCACGG ACTAAGCATG 
TACCAGGAGA TCGGGTTCCA GAAGGACAGT CAGGGAGAGT ACAAGGCATC CCAGTGCATC 
CACATGGACT GTCTCAGGTG GGTGAAGAGG GACAGTTACC TTCCTGTGGG CAGCCACAAC 
CTCAAGGCAG CTGCCAAAGC CAAGCTAGGC TATGACCCAG TGGAGCTGGA CCCTGAGGAC 
ATGTGCCGAA TGGCCACCGA GCAGCCCCAG ACTCTGGCCA CGTACTCCGT GTCAGATGCA 
GTGGCCACGT ACTACCTGTA CATGAAATAC GTCCACCCCT TTATATTCGC CCTTTGCACC 
ATTATCCCCA TGGAGCCTGA TGAGGTGCTG CGGAAGGGCT CTGGGACTCT CTGTGAGGCC 
TTGCTGATGG TGCAGGCCTT CCACGCCAAC ATCATCTTCC CCAATAAGCA GGAGCAGGAG 
TTCAACAAGC TGACAGGTGA TGGCCACGTG CTGGATGCCG AGACCTATGT GGGTGGCCAC 
GTGGAGGCCC TTGAGTCAGG CGTCTTTCGC AGTGACATCC CCTGCCGGTT CAGGATGAAT 
CCTGCTGCCT TTGACTTCCT GCTGCAGAGG GTGGAGAAGA CCATGCGCCA TGCCATCGAG 
GAAGAGGAGA AGGTGCCCAT GGAGCATGTC ACCAACTTCC AAGAGGTGTG TGATCAGATT 
AAGACCAAGC TCACCTCCCT GAAAGATGTT CCAAACAGAA TAGAGTGTCC CCTTATCTAC 
CACCTGGATG TGGGGGCCAT GTACCCTAAC ATCATCCTGA CGAACCGCCT GCAGCCCTCT 
GCCGTGGTGG ATGAGGCTAC CTGTGCTGCC TGTGACTTCA ATAAGCCTGG GGCCAACTGC 
CAGAGAAAGA TGGCCTGGCA GTGGAGGGGC GAGTTCATGC CAGCCAGTCG CAGTGAGTAC 
CATCGGATCC AGCACCAGCT GGAGTCAGAG AAGTTCCCTC CCTTGACTGC AGAGGGTCCA 
GCCCGGGCCT TTCACGAGCT CTCTCGAGAG GAGCAGGCTA AATATGAGAA GCGGAGGCTG 
TCAGATTACT GCCGGAAGGC ATACAAGAAG ATACACGTCA CCAGGGTGGA GGAGCGCCTC 
ACCACTATCT GCCAGCGGGA GAACTCTTTC TATGTGGACA CAGTGCGCGC CTTCCGAGAC 
AGGCGTTATG AATTCAAAGG TCTCCACAAG GTGTGGAAGA AGAAACTCTC TGCAGCCATG 
GAGGTGGGTG ATGCGGCCGA AGTGAAGCGC TGCAAGAACA TGGAGGTCCT GTATGACTCC 
CTGCAGCTGG CGCACAAGTG CATCCTCAAC TCCTTCTATG GCTACGTCAT GCGCAGAGGG 
GCTCGCTGGT ACTCCATGGA GATGGCAGGC ATTGTCTGCT TCACAGGGGC TAACATCATC 
ACCCAGGCAC GGGAGCTGAT TGAGCAGATC GGGAGACCCT TGGAGCTGGA CACAGACGGA 
ATATGGTGCG TCCTGCCCAA CAGCTTTCCT GAAAACTTTG TCATCAAGAC AACCAGTGTG 
AAAAAGCCCA AGGTGACCAT TTCCTACCCT GGGGCCATGT TGAACATCAT GGTGAAGGAA 
GGCTTCACCA ATGATCAGTA CCAGGAGCTG ACTGAACCAT CCTCACTCAC CTATGTCACC 
CGTTCAGAGA ACAGCATCTT TTTCGAGGTT GACGGACCCT ACCTTGCCAT GATCCTTCCA 
GCCTCCAAGG AAGAAGGCAA GAAACTGAAG AAGAGATATG CTGTCTTCAA CGAGGACGGT 
TCCCTGGCTG AGCTGAAGGG CTTCGAGATC AAACGCCGAG GGGAGCTGCA GCTGATTAAG 
ATCTTCCAGT CCTCAGTGTT CGAGGCTTTC CTCAAGGGCA GCACCTTAGA AGAAGTGTAT 
GGCTCTGTTG CCAAGGTGGC TGACTACTGG CTGGACGTGC TGTACAGTAA GGCAGCTAAC 
ATGCCCGATT CTGAGCTGTT TGAGCTGATC TCTGAGAATC GCTCCATGTC TCGGAAGCTG 
GAAGATTATG GGGAGCAGAA GTCCACGTCT ATCAGCACAG CCAAGCGCCT GGCTGAGTTC 
CTGGGAGATC AGATGGTGAA GGATGCAGGG CTGAGCTGCC GTTACATCAT CTCTCGGAAG 
CCCGAGGGCT CTCCGGTCAC AGAGAGGGCC ATCCCGCTTG CCATTTTCCA GGCAGAGCCC 
ATGGTGAGGA AGCACTTTCT CCGGAAATGG CTTAAGAGTT CTTCCCTCCA AGACTTTGAT 
ATTCGCACGA TTTTGGATTG GGAGTACTAC ATTGAGCGGC TAGGGAGTGC CATACAGAAG 
ATCATCACGA TCCCTGCAGC TTTGCAGCAG GTAAAGAACC CAGTACCACG CGTCAAGCAC 
CCTGACTGGC TGCACAAGAA ACTTTTGGAG AAGAATGATG TCTGCAAGCA GAAGAAGATC 
AGCGAGCTCT TTGTCCTCGA GGGCAGGAGA CAGGTGGGCA TGGCCCAGGT TCCAGAAGGC 
ACCCAAAGCC TGGGTGCTCC TGATATGGAA GATTTTGGCC TCATGAAGCC ATTGCACTTG 
GCCGTTCCTA TTGCCACTAA GAGGAAGCGT GTCCTCTGGG AGAGCCAGGA GGAGTCACAC 
GACCTGGAGC TGACGGTGCC CTGGCAGGAA ATCTTGGGGC AGCCTCCAGC CCTTGGAACC 
ACCCAGGAAG AGTGGCTGGT TTGGCTCCGG TTCCATAAGA AGAAGTGGCA GCTGCAGGCC 
CGGCAGCGCC TGGCTTGCAA GAAGAGGCGG TGTGTGGAGG TGGCAGAGGG CGAACCTCTG 
CCTGGGGCCA TCCGAGAAAG GCCTGCCACT GGGTTGGGGG GCTTCCTGCG AAGGACCGCC 
CGCAGCATCT TGGACCTTCC ATGGCAGATT GTGCAGATCA GTGAAACCAG CCAGGCTGGT 
TTATTCAGGC TGTGGGCTGT CATCAGCAGT GACTTGTACT GCATCAGGCT GAACATCCCC 
CGAGTATTCT ATGTCAACCA GCAGGTGGCT AAAGCTGAGG AGGGGCCTTC CTATCGCAAG 
GTCAGCCGAG TCCTTCCTCG CTCCAACATG GTCTACAATC TCTATGAGTA CTCCGTGCCT 
GAGGACATGT ACCAGGAACA TATCAATGAA ATCAATACTG AGTTATCAGC CCCAGACATT 
GAGGGCGTGT ATGAGACTCA GGTTCCATTA CTATTCCGCG CCCTAGTGCA GCTGGGCTGT 
GTCTGTGTGG TCAATAAACA GCTGGTGAGG CACCTTTCAG GCTGGGAAGC AGAAACCTTT 
GCTCTCGAGC ACCTGGAGAT GCGTTCTCTG GCTCAGTTCA GCTACCTGGA ACCAGGGAGC 
ATCCGCCACC TCTACCTGTA CCATCATGCA CAGGGCCACA AAGCACTCTT CGGCTTGTTC 
GTGCCCTCCC AGCGCAGAGC ATCTGTGTTT GTGTTGGACA CTGTGCGAAG CAACCAGATG 
CCCAGCCTCA GCGCTCTTTA CTCAGCCGAG CACAGCCTTC TGATGGAGAA AGTGGGCCCG 
GAGCTCCTGC CTCCTCCCAA ACACACTTTT GAAGTTCGGG CGGAAACTGA CATGAAAACC 
ATCTGCAGAG CCATCCAGCG CTTCCTGCTG GCCTACAAAG AGGAGCGCCG TGGGCCCACA 
CTCATCGCTG TTCAGTCCAA CTGGGAGCTA AAGAGGCTGG CTGGTGAGGT TCCTGTCCTA 
GAGGAATTCC CACTAGTGCC TGTCCACGTG GCAGATAAGA TCAGCTATGG AGTCCTGGAC 
TGGCAGCGCC TCGGAGCCCG GCGCATGATC CGCCACTACC TCAACCTGGA CACCTCTCTG 
TCACAGGCCT TCGAGATGAG CAGGTACTTC CACATCCCCA TTGGTAATCT GCCTGAGGAC 
ATCTCCACCT TTGGCTCCGA CCTCTTCTTT GCCCGCCATC TCCAGCGCCA CAGCCACCTG 
CTCTGGCTGT CGCCTACGTC ACGCCCTGAC CTGGGTGGCA AGGAGGCTGA TGACAATCGC 
CTTGTCATGG AATTCGATGA CCAGGCCAGC GTGGAGATCA ATTGTCCAGG CTGCTACTCT 
ACAGTGTGTG TGGAGCTGGA CATCCAAAAC CTGGCTGTCA ACACCATCCT GCAGTCCCAC 
CATATCAATG ACATGGAGGG GGCTGACAGC ATGGGCATCA GCTTTGATGT GATCCAGCAA 
GCCTCTCTGG AGGACATGAT CACTGGCAAC CAGGCCGCCA GCACTCCAGC CAGCTACGAT 
GAGACAGCCC TCTGCTCCAG CACCTTCAGG ATCCTGAAGA GCATGGTGGT GGGCTGGGTG 
AAGGAGATCA CACAGTACCG CAACATCTAT GCTGACAACC AGGTGATGCA CTTTTACCGC 
TGGCTCCGGT CCCCATCATC ACTGCTCCAT GACCCTGTGC TGCATCGCAC ACTCCACAAC 
ATGATGAAGA AGCTCTTTCT GCACATCCAT TCTAAAGAGA TCTTCCATTC TTTGACAATA 
TCTTTCTCTC GGTGCTGGGA ATTTCTTCTC TGGATGGATC CCTCTAACTA TGGTGGAATC 
AAAGGAAATG CTCCATCTAG TATCCACTAT GGACAGCAAG ACTCCCAAAA AGGGGGCAGA 
GTAGAGGAGG AGGAGGAGGA GGAGGAGGAG GAAGAGGAGG AGGAAGGCAA ACAGGAACCA 
GATGTGGAGG ACATGCTGGA AAACAACTGG AACATTGTGC AGTTCCTGCC ACAGGCAGCC 
TCCTGCCAGA GCTACTTCCT CATGATTGTT TCAGCATACA TCGTGGCCGT GTACCACAGC 
ATGAAGGAGG AGCTGCGGCG CAGTGCCCCA GGGGGCACAC CCCTGAGATG GCGGGGGCCC 
AGCCAGCTCT CCCAGGATGC TGCGGCCTCC AGTGCCCTTC CTGCTATGGT CCCTGCCCTC 
CTAGGAATGA TCACCTTCTC CCAAGACTAC GTGGCCAACG AGCTGACTCA GAACTTCTTC 
ACCATCACTC AGAAGATTCA GAAGAAAGTC ACAGGCTCCC GGAGCTCCAC TGAGCTCTCC 
AGCATGTTTC CTGCCCTTCC AGGTTCTCAC TTGCTGCTCA AAAACCCCGC ATTGGAGTTC 
ATCAAGTACG TGTGCAAGGT TCTGTCTCTG GACACGAACA TCACAAACCA GGTGAACAAA 
TTGAACCGGG ATCTGCTTCG TCTGGTGGAT GTTGGCGAGT TCTCGGAGGA GGCACAGTTC 
CGAGACCCTT GCCGTTCCTA CGTGCTCCCT GAGGTCATCT GCCGCAGCTG TAACTTCTGC 
CGGGACCTGG ACCTGTGTAA AGAGTTCGCC TTCTCGCAGG ATGGGGCCGT GCTGCCTCAG 
TGGCTCTGCT CAAACTGTCA GGTTGCCTAT GACACCTCAG TCATCGAGAT GGCCCTGGTG 
GAAGCCCTGC AGAAGAAACT GATGGCCTTC ACTCTGCAGG ACCTGATCTG CCTGAAGTGC 
CGAGGTGTGA AGGAGACCAA CATGCCTGTG TACTGCAGCT GCGCCGGGGG CTTTACCCTC 
ACCATCCACA CCAAGGTCTT CATGGAGCAG ATTGGAATCT TCCAGAACAT TGCCCAGCAC 
TATGGCATGT CCTACCTCAC AGAGACCCTG GAGTGGCTGC TGCAGAAGAA CCCTCAGCTG 
GGCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013963546.1
CDS	56..6664
Translation	MDLRFGFERLKESGEKTGWLINMHPTEILDEDKRLVSAVDYYFIQDDGSRFKVALPYEPYFYIAARKGCEREVSSFLSKKFQGRIAKIETVPKEDLDLPNHLVGLKRNYIKLSFHTVEDLVRVRKDVSPAVKKNREQGHAGDAYTAMLSSVLQGGSVVIDEEEASKKVTDQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNIRYRGSAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDVEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFDYVQEMKPTIMVTYNGDFFDWPFVEARAAVHGLSMYQEIGFQKDSQGEYKASQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTGDGHVLDAETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTMRHAIEEEEKVPMEHVTNFQEVCDQIKTKLTSLKDVPNRIECPLIYHLDVGAMYPNIILTNRLQPSAVVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLTAEGPARAFHELSREEQAKYEKRRLSDYCRKAYKKIHVTRVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAMEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRRGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTSVKKPKVTISYPGAMLNIMVKEGFTNDQYQELTEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEIKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPMVRKHFLRKWLKSSSLQDFDIRTILDWEYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVCKQKKISELFVLEGRRQVGMAQVPEGTQSLGAPDMEDFGLMKPLHLAVPIATKRKRVLWESQEESHDLELTVPWQEILGQPPALGTTQEEWLVWLRFHKKKWQLQARQRLACKKRRCVEVAEGEPLPGAIRERPATGLGGFLRRTARSILDLPWQIVQISETSQAGLFRLWAVISSDLYCIRLNIPRVFYVNQQVAKAEEGPSYRKVSRVLPRSNMVYNLYEYSVPEDMYQEHINEINTELSAPDIEGVYETQVPLLFRALVQLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHLYLYHHAQGHKALFGLFVPSQRRASVFVLDTVRSNQMPSLSALYSAEHSLLMEKVGPELLPPPKHTFEVRAETDMKTICRAIQRFLLAYKEERRGPTLIAVQSNWELKRLAGEVPVLEEFPLVPVHVADKISYGVLDWQRLGARRMIRHYLNLDTSLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHSHLLWLSPTSRPDLGGKEADDNRLVMEFDDQASVEINCPGCYSTVCVELDIQNLAVNTILQSHHINDMEGADSMGISFDVIQQASLEDMITGNQAASTPASYDETALCSSTFRILKSMVVGWVKEITQYRNIYADNQVMHFYRWLRSPSSLLHDPVLHRTLHNMMKKLFLHIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGNAPSSIHYGQQDSQKGGRVEEEEEEEEEEEEEEGKQEPDVEDMLENNWNIVQFLPQAASCQSYFLMIVSAYIVAVYHSMKEELRRSAPGGTPLRWRGPSQLSQDAAASSALPAMVPALLGMITFSQDYVANELTQNFFTITQKIQKKVTGSRSSTELSSMFPALPGSHLLLKNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCKEFAFSQDGAVLPQWLCSNCQVAYDTSVIEMALVEALQKKLMAFTLQDLICLKCRGVKETNMPVYCSCAGGFTLTIHTKVFMEQIGIFQNIAQHYGMSYLTETLEWLLQKNPQLGH

Target ORF information:

RefSeq Version	XM_014108071.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014108071.1

ORF Insert Sequence:

ATGGATTTGC GGTTTGGTTT TGAGAGACTC AAGGAGTCTG GGGAGAAGAC GGGCTGGCTT 
ATCAACATGC ACCCTACTGA GATTTTAGAT GAAGACAAAC GCTTAGTCAG CGCAGTAGAT 
TACTACTTTA TTCAAGATGA TGGGAGCAGA TTTAAGGTGG CCTTGCCCTA CGAGCCGTAT 
TTCTACATTG CAGCCAGAAA GGGTTGTGAA CGAGAAGTTT CATCTTTTCT CTCCAAGAAG 
TTTCAGGGTA GAATTGCTAA AATAGAGACT GTCCCCAAAG AGGATCTAGA CTTGCCAAAT 
CACTTGGTGG GTTTGAAGCG AAATTACATT AAGCTGTCCT TCCACACTGT GGAGGATCTT 
GTTAGAGTGA GGAAGGATGT CTCCCCTGCT GTGAAGAAGA ACCGGGAGCA GGGTCATGCT 
GGTGATGCCT ACACAGCTAT GCTGTCCAGC GTTCTGCAAG GGGGCAGTGT GGTTATTGAT 
GAAGAGGAAG CCTCTAAGAA GGTCACTGAC CAGTTGGACA ATATTGTGGA CATGCGAGAG 
TATGATGTGC CCTACCACAT CCGCCTCTCC ATTGACCTGA AGATCCATGT GGCTCATTGG 
TACAACATCA GATACCGAGG AAGTGCCTTT CCTGTGGAAA TCACCCGCCG AGATGACCTT 
GTTGAACGAC CTGACCCTGT GGTTTTGGCA TTTGACATTG AGACAACCAA ACTGCCTCTC 
AAGTTTCCTG ATGCTGAGAC TGACCAGATT ATGATGATTT CCTACATGAT TGATGGTCAG 
GGCTACCTCA TCACCAACAG GGAGATCGTT TCAGAAGATG TCGAAGATTT CGAGTTCACC 
CCCAAGCCAG AGTATGAAGG ACCTTTTTGT GTCTTTAATG AACCTGACGA GGTTCATCTA 
ATCCAGAGAT GGTTTGACTA TGTCCAGGAG ATGAAACCCA CCATCATGGT CACCTACAAT 
GGGGACTTTT TTGACTGGCC GTTTGTGGAG GCCAGAGCAG CAGTCCACGG ACTAAGCATG 
TACCAGGAGA TCGGGTTCCA GAAGGACAGT CAGGGAGAGT ACAAGGCATC CCAGTGCATC 
CACATGGACT GTCTCAGGTG GGTGAAGAGG GACAGTTACC TTCCTGTGGG CAGCCACAAC 
CTCAAGGCAG CTGCCAAAGC CAAGCTAGGC TATGACCCAG TGGAGCTGGA CCCTGAGGAC 
ATGTGCCGAA TGGCCACCGA GCAGCCCCAG ACTCTGGCCA CGTACTCCGT GTCAGATGCA 
GTGGCCACGT ACTACCTGTA CATGAAATAC GTCCACCCCT TTATATTCGC CCTTTGCACC 
ATTATCCCCA TGGAGCCTGA TGAGGTGCTG CGGAAGGGCT CTGGGACTCT CTGTGAGGCC 
TTGCTGATGG TGCAGGCCTT CCACGCCAAC ATCATCTTCC CCAATAAGCA GGAGCAGGAG 
TTCAACAAGC TGACAGGTGA TGGCCACGTG CTGGATGCCG AGACCTATGT GGGTGGCCAC 
GTGGAGGCCC TTGAGTCAGG CGTCTTTCGC AGTGACATCC CCTGCCGGTT CAGGATGAAT 
CCTGCTGCCT TTGACTTCCT GCTGCAGAGG GTGGAGAAGA CCATGCGCCA TGCCATCGAG 
GAAGAGGAGA AGGTGCCCAT GGAGCATGTC ACCAACTTCC AAGAGGTGTG TGATCAGATT 
AAGACCAAGC TCACCTCCCT GAAAGATGTT CCAAACAGAA TAGAGTGTCC CCTTATCTAC 
CACCTGGATG TGGGGGCCAT GTACCCTAAC ATCATCCTGA CGAACCGCCT GCAGCCCTCT 
GCCGTGGTGG ATGAGGCTAC CTGTGCTGCC TGTGACTTCA ATAAGCCTGG GGCCAACTGC 
CAGAGAAAGA TGGCCTGGCA GTGGAGGGGC GAGTTCATGC CAGCCAGTCG CAGTGAGTAC 
CATCGGATCC AGCACCAGCT GGAGTCAGAG AAGTTCCCTC CCTTGACTGC AGAGGGTCCA 
GCCCGGGCCT TTCACGAGCT CTCTCGAGAG GAGCAGGCTA AATATGAGAA GCGGAGGCTG 
TCAGATTACT GCCGGAAGGC ATACAAGAAG ATACACGTCA CCAGGGTGGA GGAGCGCCTC 
ACCACTATCT GCCAGCGGGA GAACTCTTTC TATGTGGACA CAGTGCGCGC CTTCCGAGAC 
AGGCGTTATG AATTCAAAGG TCTCCACAAG GTGTGGAAGA AGAAACTCTC TGCAGCCATG 
GAGGTGGGTG ATGCGGCCGA AGTGAAGCGC TGCAAGAACA TGGAGGTCCT GTATGACTCC 
CTGCAGCTGG CGCACAAGTG CATCCTCAAC TCCTTCTATG GCTACGTCAT GCGCAGAGGG 
GCTCGCTGGT ACTCCATGGA GATGGCAGGC ATTGTCTGCT TCACAGGGGC TAACATCATC 
ACCCAGGCAC GGGAGCTGAT TGAGCAGATC GGGAGACCCT TGGAGCTGGA CACAGACGGA 
ATATGGTGCG TCCTGCCCAA CAGCTTTCCT GAAAACTTTG TCATCAAGAC AACCAGTGTG 
AAAAAGCCCA AGGTGACCAT TTCCTACCCT GGGGCCATGT TGAACATCAT GGTGAAGGAA 
GGCTTCACCA ATGATCAGTA CCAGGAGCTG ACTGAACCAT CCTCACTCAC CTATGTCACC 
CGTTCAGAGA ACAGCATCTT TTTCGAGGTT GACGGACCCT ACCTTGCCAT GATCCTTCCA 
GCCTCCAAGG AAGAAGGCAA GAAACTGAAG AAGAGATATG CTGTCTTCAA CGAGGACGGT 
TCCCTGGCTG AGCTGAAGGG CTTCGAGATC AAACGCCGAG GGGAGCTGCA GCTGATTAAG 
ATCTTCCAGT CCTCAGTGTT CGAGGCTTTC CTCAAGGGCA GCACCTTAGA AGAAGTGTAT 
GGCTCTGTTG CCAAGGTGGC TGACTACTGG CTGGACGTGC TGTACAGTAA GGCAGCTAAC 
ATGCCCGATT CTGAGCTGTT TGAGCTGATC TCTGAGAATC GCTCCATGTC TCGGAAGCTG 
GAAGATTATG GGGAGCAGAA GTCCACGTCT ATCAGCACAG CCAAGCGCCT GGCTGAGTTC 
CTGGGAGATC AGATGGTGAA GGATGCAGGG CTGAGCTGCC GTTACATCAT CTCTCGGAAG 
CCCGAGGGCT CTCCGGTCAC AGAGAGGGCC ATCCCGCTTG CCATTTTCCA GGCAGAGCCC 
ATGGTGAGGA AGCACTTTCT CCGGAAATGG CTTAAGAGTT CTTCCCTCCA AGACTTTGAT 
ATTCGCACGA TTTTGGATTG GGAGTACTAC ATTGAGCGGC TAGGGAGTGC CATACAGAAG 
ATCATCACGA TCCCTGCAGC TTTGCAGCAG GTAAAGAACC CAGTACCACG CGTCAAGCAC 
CCTGACTGGC TGCACAAGAA ACTTTTGGAG AAGAATGATG TCTGCAAGCA GAAGAAGATC 
AGCGAGCTCT TTGTCCTCGA GGGCAGGAGA CAGGTGGGCA TGGCCCAGGT TCCAGAAGGC 
ACCCAAAGCC TGGGTGCTCC TGATATGGAA GATTTTGGCC TCATGAAGCC ATTGCACTTG 
GCCGTTCCTA TTGCCACTAA GAGGAAGCGT GTCCTCTGGG AGAGCCAGGA GGAGTCACAC 
GACCTGGAGC TGACGGTGCC CTGGCAGGAA ATCTTGGGGC AGCCTCCAGC CCTTGGAACC 
ACCCAGGAAG AGTGGCTGGT TTGGCTCCGG TTCCATAAGA AGAAGTGGCA GCTGCAGGCC 
CGGCAGCGCC TGGCTTGCAA GAAGAGGCGG TGTGTGGAGG TGGCAGAGGG CGAACCTCTG 
CCTGGGGCCA TCCGAGAAAG GCCTGCCACT GGGTTGGGGG GCTTCCTGCG AAGGACCGCC 
CGCAGCATCT TGGACCTTCC ATGGCAGATT GTGCAGATCA GTGAAACCAG CCAGGCTGGT 
TTATTCAGGC TGTGGGCTGT CATCAGCAGT GACTTGTACT GCATCAGGCT GAACATCCCC 
CGAGTATTCT ATGTCAACCA GCAGGTGGCT AAAGCTGAGG AGGGGCCTTC CTATCGCAAG 
GTCAGCCGAG TCCTTCCTCG CTCCAACATG GTCTACAATC TCTATGAGTA CTCCGTGCCT 
GAGGACATGT ACCAGGAACA TATCAATGAA ATCAATACTG AGTTATCAGC CCCAGACATT 
GAGGGCGTGT ATGAGACTCA GGTTCCATTA CTATTCCGCG CCCTAGTGCA GCTGGGCTGT 
GTCTGTGTGG TCAATAAACA GCTGGTGAGG CACCTTTCAG GCTGGGAAGC AGAAACCTTT 
GCTCTCGAGC ACCTGGAGAT GCGTTCTCTG GCTCAGTTCA GCTACCTGGA ACCAGGGAGC 
ATCCGCCACC TCTACCTGTA CCATCATGCA CAGGGCCACA AAGCACTCTT CGGCTTGTTC 
GTGCCCTCCC AGCGCAGAGC ATCTGTGTTT GTGTTGGACA CTGTGCGAAG CAACCAGATG 
CCCAGCCTCA GCGCTCTTTA CTCAGCCGAG CACAGCCTTC TGATGGAGAA AGTGGGCCCG 
GAGCTCCTGC CTCCTCCCAA ACACACTTTT GAAGTTCGGG CGGAAACTGA CATGAAAACC 
ATCTGCAGAG CCATCCAGCG CTTCCTGCTG GCCTACAAAG AGGAGCGCCG TGGGCCCACA 
CTCATCGCTG TTCAGTCCAA CTGGGAGCTA AAGAGGCTGG CTGGTGAGGT TCCTGTCCTA 
GAGGAATTCC CACTAGTGCC TGTCCACGTG GCAGATAAGA TCAGCTATGG AGTCCTGGAC 
TGGCAGCGCC TCGGAGCCCG GCGCATGATC CGCCACTACC TCAACCTGGA CACCTCTCTG 
TCACAGGCCT TCGAGATGAG CAGGTACTTC CACATCCCCA TTGGTAATCT GCCTGAGGAC 
ATCTCCACCT TTGGCTCCGA CCTCTTCTTT GCCCGCCATC TCCAGCGCCA CAGCCACCTG 
CTCTGGCTGT CGCCTACGTC ACGCCCTGAC CTGGGTGGCA AGGAGGCTGA TGACAATCGC 
CTTGTCATGG AATTCGATGA CCAGGCCAGC GTGGAGATCA ATTGTCCAGG CTGCTACTCT 
ACAGTGTGTG TGGAGCTGGA CATCCAAAAC CTGGCTGTCA ACACCATCCT GCAGTCCCAC 
CATATCAATG ACATGGAGGG GGCTGACAGC ATGGGCATCA GCTTTGATGT GATCCAGCAA 
GCCTCTCTGG AGGACATGAT CACTGGCAAC CAGGCCGCCA GCACTCCAGC CAGCTACGAT 
GAGACAGCCC TCTGCTCCAG CACCTTCAGG ATCCTGAAGA GCATGGTGGT GGGCTGGGTG 
AAGGAGATCA CACAGTACCG CAACATCTAT GCTGACAACC AGGTGATGCA CTTTTACCGC 
TGGCTCCGGT CCCCATCATC ACTGCTCCAT GACCCTGTGC TGCATCGCAC ACTCCACAAC 
ATGATGAAGA AGCTCTTTCT GCACATCCAT TCTAAAGAGA TCTTCCATTC TTTGACAATA 
TCTTTCTCTC GGTGCTGGGA ATTTCTTCTC TGGATGGATC CCTCTAACTA TGGTGGAATC 
AAAGGAAATG CTCCATCTAG TATCCACTAT GGACAGCAAG ACTCCCAAAA AGGGGGCAGA 
GTAGAGGAGG AGGAGGAGGA GGAGGAGGAG GAAGAGGAGG AGGAAGGCAA ACAGGAACCA 
GATGTGGAGG ACATGCTGGA AAACAACTGG AACATTGTGC AGTTCCTGCC ACAGGCAGCC 
TCCTGCCAGA GCTACTTCCT CATGATTGTT TCAGCATACA TCGTGGCCGT GTACCACAGC 
ATGAAGGAGG AGCTGCGGCG CAGTGCCCCA GGGGGCACAC CCCTGAGATG GCGGGGGCCC 
AGCCAGCTCT CCCAGGATGC TGCGGCCTCC AGTGCCCTTC CTGCTATGGT CCCTGCCCTC 
CTAGGAATGA TCACCTTCTC CCAAGACTAC GTGGCCAACG AGCTGACTCA GAACTTCTTC 
ACCATCACTC AGAAGATTCA GAAGAAAGTC ACAGGCTCCC GGAGCTCCAC TGAGCTCTCC 
AGCATGTTTC CTGCCCTTCC AGGTTCTCAC TTGCTGCTCA AAAACCCCGC ATTGGAGTTC 
ATCAAGTACG TGTGCAAGGT TCTGTCTCTG GACACGAACA TCACAAACCA GGTGAACAAA 
TTGAACCGGG ATCTGCTTCG TCTGGTGGAT GTTGGCGAGT TCTCGGAGGA GGCACAGTTC 
CGAGACCCTT GCCGTTCCTA CGTGCTCCCT GAGGTCATCT GCCGCAGCTG TAACTTCTGC 
CGGGACCTGG ACCTGTGTAA AGAGTTCGCC TTCTCGCAGG ATGGGGCCGT GCTGCCTCAG 
TGGCTCTGCT CAAACTGTCA GGTTGCCTAT GACACCTCAG TCATCGAGAT GGCCCTGGTG 
GAAGCCCTGC AGAAGAAACT GATGGCCTTC ACTCTGCAGG ACCTGATCTG CCTGAAGTGC 
CGAGGTGTGA AGGAGACCAA CATGCCTGTG TACTGCAGCT GCGCCGGGGG CTTTACCCTC 
ACCATCCACA CCAAGGTCTT CATGGAGCAG ATTGGAATCT TCCAGAACAT TGCCCAGCAC 
TATGGCATGT CCTACCTCAC AGAGACCCTG GAGTGGCTGC TGCAGAAGAA CCCTCAGCTG 
GGCCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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POLE cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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