pole-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	pole-1
Entrez Gene ID	173368
Full Name	DNA polymerase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_061215.3	NP_493616.1	DNA polymerase epsilon catalytic subunit
NM_061215.4	NP_493616.1	DNA polymerase epsilon catalytic subunit

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pole	NP_001121995.1
Arabidopsis thaliana (thale cress)	TIL2	NP_180280.2
Arabidopsis thaliana (thale cress)	TIL1	NP_172303.5
Oryza sativa (rice)	Os02g0511900	NP_001046939.2
Caenorhabditis elegans (roundworm)	F33H2.5	NP_493616.1
Canis lupus familiaris (dog)	POLE	XP_543348.3
Mus musculus (house mouse)	Pole	NP_035262.2
Kluyveromyces lactis	KLLA0C02585g	XP_452312.1
Neurospora crassa	NCU04548	XP_955939.2
Xenopus tropicalis (tropical clawed frog)	pole	XP_004910692.1
Drosophila melanogaster (fruit fly)	DNApol-epsilon	NP_524462.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004615	XP_315205.4
Saccharomyces cerevisiae (baker's yeast)	POL2	NP_014137.1
Eremothecium gossypii	AGOS_AFR657C	NP_986205.2
Magnaporthe oryzae (rice blast fungus)	MGG_03850	XP_003720041.1
Homo sapiens (human)	POLE	NP_006222.2
Pan troglodytes (chimpanzee)	POLE	XP_522564.4
Bos taurus (cattle)	POLE	NP_001178358.1
Rattus norvegicus (Norway rat)	Pole	NP_001100622.2
Gallus gallus (chicken)	POLE	XP_004934482.1
Schizosaccharomyces pombe (fission yeast)	cdc20	NP_596354.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pole-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pole-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa335775	NM_061215.4 Latest version!	Caenorhabditis elegans DNA polymerase epsilon catalytic subunit (pole-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCa335775	NM_061215.3	Caenorhabditis elegans DNA polymerase epsilon catalytic subunit (pole-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pole-1 ( NM_061215.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_493616.1 Caenorhabditis elegans DNA polymerase epsilon catalytic subunit (pole-1), partial mRNA.

Price & Availability↑

CloneID	OCa335775
Clone ID Related Accession (Same CDS sequence)	NM_061215.3 , NM_061215.4
Accession Version	NM_061215.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	DNA polymerase epsilon catalytic subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Jun 26, 2012 this sequence version replaced NM_061215.2. COMPLETENESS: incomplete on both ends.

ATGAGCTCCA AGGATGACAT TCTTGCACAG GCGGTCGAAA ATGATTCAAA CTACAAAGAG 
CGGCTTGCCC TGATCAGATC AAATGATGAG ATTGATGCGA AGCTTGGTTT CTCTAGATAC 
ACAGGACTAC AGGAGAAAAA AGGATTTCTG ATAAACATCC AACCTTCGGA GCTTGTCGAC 
GAGCAAACCA AAGTTATTAT TTCGGTCGTT GACTATTTCT TCATCAGTGA TATGGACGAA 
CGCTTCAAAA TCAGTTACCC ATTTCGACCG TATTTCTATA TTGCCACGTT GGATGGATTT 
GAATTCCAAG TATCCAGTTA TCTGTCCAAG AAGTACGGAG CCCAGACAGC AGTTGAACAT 
ATGGATCGGG AGGATTTAGA TTTGAAAGAT CATCTTTCTG GGTTGAAGAA GACATATATC 
AAACTCTCAT TCACCAGCAC CGTGGAATTG ATAAAAATCC GTAAGGAATT AATGCCGCTG 
GTGCGGAAAA ACACAGATCG CATCAAAAAA GAGTCGGCGT ACGCTGACTA TTTGGCGAGG 
AATCTGAGTG GAAAAGGAGG AGATAGTAAG GATCAACAGT TGAATGGTGA CATCTTGAAT 
CAAATTGTTG ATATCAGAGA ATACGACGTG CCTTTTCACA TGAGAGTTTC CATCGATGAA 
AAGATCTTCG TCGGATTATG GTACGATGTG AAAGGAATTG GCCCGAACCG TGTTCCAACA 
ATAAAGCGAA AAGATTTGCC CTTGTTCCAC GCAAAGCCGA AAGTTCTCGC CTTTGACATT 
GAAACAACGA AACTTCCGTT GAAATTTCCG GATCGTGAGA GCGATGAAAT TATGATGATA 
TCATATATGG TTGATGGACG TGGATTTTTG ATTATCAACC GAGAAATCGT GTCGGCTGAT 
ATTAATGCGT TCGAATACAC ACCGAAAGCC GAGTACATAG GAGAATTCAC AGTCTGGAAT 
GAGAAGGACG AAGCTGCTCT GATTCGGAAA TTTTTCGATC ATTTTTTGCA GGTTCGTCCA 
AATATTGTTG TCACCTACAA TGGAGATTTC TTCGATTGGC CATTCGTAGA AGCTCGTGCC 
AAGATCCGCG GATTCAATAT GGAAAGGGAA ATCGGATTTT CTAAGGATTC CGCCGATGAG 
TACAAATCAC GAAATTGTAT TCATATGGAC GCTTTCCGAT GGGTCAAACG TGACTCATAT 
CTACCGGTTG GAAGTCAGAA TCTGAAGGCA GTGACGAAGG CAAAGCTGAG ATATGATCCA 
GTTGAGGTGG AGCCGGAACT GATGTGCAAA ATGGCTCGAG AGCAGCCTCA ACAGCTGGCC 
AATTACTCGG TTTCGGATGC AGTGTCCACT TATTATCTCT ACATGAAGTA TGTTCATCAA 
TTTATCTTCG CTTTGTGCAC AATTATCCCA CTAGGAGCAG ATGACGTGTT GAGAAAAGGA 
TCTGGAACAC TTTGTGAAGC TCTTCTGATG GTTGAAGCAT TCCATAACAA TATTGTGTTC 
CCGAACAAGT ACACTGGACC AGAGGAGACA AGATTCAGTA AAGATGGACA TCGTGTTGAG 
AGCGAGACCT ATGTCGGAGG ACACGTGGAA GCTCTGGAGG CCGGCGTATT CAGAGCGGAT 
ATTCCAGCAA AATTCCGGCT TTCCGTTCCT GCTCTTGAGC AATTAAAATC CGAGATTCAA 
GAGACCCTTC GCAAGGAGCT GGCTCGAGAG TTTGAGGTCA CTCTTGACCA GGTCGTTGAC 
TTTGATGAGC AGTGCGCTGA AGTTCAAGAT GCATTTGATG GAATGATAAA TGTGCCAACA 
CGCCTCGAGA ATCCACGTAT CTACCATTTG GACGTCGGGG CCATGTACCC CAACATCATT 
TTGACTAATC GATTGCAGCC ATGTGCGATG GTCACCGAGG AGATTTGCAT GGGATGCTCC 
TACAACAAAC CAGACGCAGA ATGCAAGAGG ACGATGGCTT GGGAATGGAG GGGAGAGCTG 
ACACCAGCCA CTCGAGGAGA ATACCAGCAA ATTATGCAGC AACTCGAAGC AGAATCGTTT 
GGAAAACCAC CGAAACACTT TCATATGCTC GAGAGATCGG AGCGAGAAGC CATCGAAATG 
AAGCGTATCA AAGATTACAG CAGGCGTGTC TACGGAAAGA CTCATTTGAC AAGATTAGAA 
ATGAGAGAAA CTACAATTTG TCAACGGGAG AATCATTTCT ACGTGGAAAC TGTAAAAGCG 
TTCCGTGATA GAAGATACGA GTACAAGGAT ATGTTGAAAA AGGCGAAAGG ACGATTCGAT 
CAAGCCCAGG CGACAAATGA TCTTGCAACT ATGACTACAT CTAAGTTGGA AATGGTGCTC 
TACGAATCAC TGCAGCTGGC TCACAAGTGC ATTCTCAACT CTTTCTACGG ATATGTGATG 
CGTAAAGGAT CCAGATGGTT TTCAATGGAG ATGGCTGGAA TTGTGTGCCA TACTGGTGCA 
AATATCATCC GAGAAGCACG CAAACTTGTT GAACAGATTG GAACACCGTT GGAGCTTGAC 
ACTGATGGAA TTTGGTGCCT GATCCCTGCT TCGTTCCCTG AAAATGTCAC TTTCAAGCTG 
AAAAATCACA AAAGAAGTTC TGTTACAGTG TCGTATCCGG GTGCAATGCT GAATGCGTTG 
GTGTATGAAG GATTCACCAA TCATCAGTAC CACACTCTGG AAAAAGATGG GTCTTACTCA 
AAATCGTCGG AGAATTCGAT TTATTTCGAA GTAGATGGTC CATATCAATG TATGATTCTG 
CCTGCATCAA AGGAAGAGGG AAAGAAATTG AAGAAACGAT ACGCCGTGTT CAATTTAGAT 
GGATCACTGG CGGAAATGAA GGGATTCGAG TTGAAGAGAC GTGGAGAGTT GAATATTATC 
AAACATTTTC AGGGATGCGT ATTCAAAACC TTCCTCAACG GAAAAACTCT TGAGGAGACG 
TACAAAGCAG TGGCAGCTGA TGCGGATCAC TGGCTGGATA TTCTTCACTC CCATGGCGCC 
GATCTTACCG ATGAAGAACT ATTCGACTTG ATTTCAGAGA ACAGAAGTAT GTCGAGAAAG 
CTGGAAGACT ATGGAGCACA AAAGAGCACA TCAATCAGTA CTGCAAAACG GCTAGCTGAA 
TTCCTCGGAG ATGATATGGT CAAAGATGCT GGACTAGCCT GCATGTTCAT CATCTCGAAG 
CATCCGATTG GTGCACCGGT TACGGAAAGA GCTATTCCAG TTGCAATCTT CAAGTCAGAT 
GCTAAAGTTC GCAGTCATTA CATCAGGAAG TGGACGAAAC AAGTGGATTT CAATGAGGAT 
ACCGATATTC GGGATATGTT GGATTGGGAT TACTATTTGG AACGATTTGG CAGTTGTATT 
CAGAAGATTA TCACAATTCC AGCCGCACTT CAAGGCATAT CAAATCCAGT GCCACGTGTA 
CCACATCCAG ATTGGTTGCA GAACAAGATA CGGAATAAAT TCGACGCTCA CAGGCAGCCA 
CGTATCAATC AGATTTTCGC CGCCTGCCAA AAACCTTCAA CTTCACAAAT GGACAATGGA 
AAACGTCGTA GAACACCTGA TGATGACGTG GCATCTGAAG ATGCTATGGA TTCACAAGAT 
GACATCATAA TCGACGATGA TAAGGAGAAT GGAGCGAAGC GGCAGAAGAA CACCAAGAAG 
GTACATACAA CTGAGGTAGT CCTCGAGAAG AAGACGTTGG TGGAACACGG TTTCGATGAA 
TGGATGGGAT TTCTGAAAAA GAAGTGGAGA GTGCAGAGAA AGGAGAGAAA AACTCAGCTA 
TCATCGAAAG ATTCAGATGT AGTGGAGGCG ATTGTTCGTG GTGCGAGAGA AGCCGAGCAT 
GACAAGGAAT GGCATATTTT GAGTGTGGAG CCGACTGCGG ACGCCTCATT TTTCAATGTT 
TGGCTAGCAG TTCAGGGACA AATGCACAAA GTGATTATGA AGATTGGTAG ACGGATCATC 
GTCGATTCTC GTGCTCCACG CGGCGATCGT GATACAATTC GACGAATTTT ACCACATCAC 
AAGACACCTG GTTTCCTTTA CGAATTCCGT ACAGATGAGA ATCAGCTGAC AGCACTCATG 
GATAAGCTGT ATTCGGAGAC GTGTAGTTCG ACGATTGATG GAATCTACGA GTCAGAAGTT 
CCCACAAGCT TCCGTGCTGT ACTCCAACTT GGCTCAATCG TTCGACCGGA TCATGGAATC 
TCGCTGGGTG GACATCAACT GACACTGGAA AATTTAAAAC CTATGGAGAA GGCACCGTAT 
CTACCACTAG ATCAGAAGAT TCGGACTATC TTCTTGTATA AGTTCTCACA GGATTCTCGG 
CATGTCTATT CGCTTATTGA TTCGTCTGGC TCCGCAGCTT ACTTCTACAT TGTTAATACT 
GGAGATGTTC AGATGCCTAA TATGGACTCC CTCTACACAT CCGCATACAC TAAAATGATG 
TCAACTGAAC GTGGTCAGCT CTGTCACACT TCTGAAAGCA TGCCGTTCAC AGTGAAGCGA 
TTCTCATCAA ATACAGAATG CGAACGTCAA TTGGGAAGAG CATTGCGTGT ATATCGAGAG 
GTTTCGTCCA AAACTGCGAT TGTTCTTTTG CTCAGTGACA CGGATCCATT CCGATTGGCA 
CGAAAACTTC CGAATCTCGG ATTATTCCCA AATGTGCAGC TTCATATCAC TGAACCATCG 
TCACTTCTCA ATCAGATAGA CTGGCAAAAA GTCGTGGCTC GTCGAGTACT GCAACACTAT 
TTCAACAGCT TCTTCTTCCT CGCCGACTAT CTTGAATGGG CTCGCTATCT TCGTGTTCCA 
ATCGGTAATC TGCCTGCTGA TCATGCGCTC TTCGGATTGG ATCTTCTCTT CGCCAGAAAC 
CTCCAGAAAT CAGGTCACGC TCTGTGGGCA ACTCGTGCGA GCCGTCCGGA CTTGGGAGGA 
AAGGAAATGG ATGATGTTCG TCTTTCAGTT GATTGGAATC CGTTATCAAT AGATGATACG 
GTTCTGTTGA ATCGGGAGAC GTTCTGTGAA ACTGCCTGTG TTGAACTTCA ACTCAGCGCT 
GTTGCCGTGA CGGCTTTAGT TCAACGAAGT CGTGTTTTGG AGGCAGAAGG GGCCGACGAT 
GTAGTCACAT TTGACTCGAT GAATACAATT GCACAACAGT CAGTGACAGG AGGAGCTGTA 
AACTCAATCG CCTGCTATGA TGAAGGAGCC GCGGTGGATG CAACGATAAA AGTGCTCAAA 
CAAATGCTCA CAGAATGCGT TCGCCATATT GCCCATCAAG GAAATGCACG TGCCGATGAA 
GTTGTGATGA CCGTATCCAG ATGGCTCAAC ACTCGATCTG CTCTACTCTT TGACGCTGCC 
CTCACCAGGA GTGTTTCGGT GCTGGAGAGT AAGTTGGTGC TACTATTGTG TGCGGAATGT 
GAACGAATTG GAGCAAAAGT TATTCACGCA ACTGCTCAAA AGCTGGTGTT GAATACAGGA 
AAATCGACAA GCGAGGAGGC CAAGGGGTTT GCGGAGATGC TCATTCAGTC GTTGAGCACA 
AATGTTGTTT TTGCGGCTCT TCATATCACT CCTGTGAAGT TTTTCGATGC GATGCTCTGG 
ATGGACGCAC ACAATCATAC TGGAATTCGA ATTTCCGAAA AGACCGAATC GTCCCCAGAT 
GTGATAGCTG ATGAAGAAGA AAGTAGCTGC ACCGAGTTCG AGACAACTGC TATTTGGAAG 
ATCGCAGAAG AAATGCCAAC AGAGGCGAAT ATTCAGGAGG AATTCCTCCA AATGATTGGC 
GCCTACATTC TTGAATTCCT TGAAACCAAT CGAAAAATGC ACTTTGACTC GGAGTCCGGT 
GCAACTTTCC GGTCAGACTG CATCTCACAA AAGATCTCAC ATCGTCTCTA TCGAATTGTG 
AACAAGATGG TGCATAACAA TGCGGATATT GCACATTGTT CTGTGTATTT GGCGAATGCG 
CTCTGTCGAG CTCTATCCTG TGATCAGACC TCCCAGTTGG CAGTCGAAGG AATTCGGGAT 
AATGCGAAGC GTCTTCTGCA CAATTCTGTT GTGGAAGCCG ACATGACACC ACTGCGTTCA 
ACGACTCTCT TCGTCTCAAA TGTCTTCTGC AATTCGTGTT CTCAGGCCTC GAACGTATTC 
CTTTCCTCCA CTGATGAGAT TCTCACATGT GCCACGTGTC AGAGTAAGCT CAACTCTGAC 
GTCATTGATA TGATGATTTG CGATCGATTG AACCAGCTGC TCACTGCCTA CCAGATTCAG 
GATCATCAGT GCACGAAATG CAAATCGGTT CGTCACGACA CACTATCAAT GTACTGCGAA 
TGCTGTTCCC AGTTCATTCC ACAAATCACA CCTGCACAGC TGAAGCACGA GGCTTCCACC 
GTCGAGACTG TCTCCATCGT CCGCAATTTC GCGTTGAGCT CCGAACTCGC CACATGGGTC 
TTGAAAATGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_493616.1
CDS	1..6435
Translation	MSSKDDILAQAVENDSNYKERLALIRSNDEIDAKLGFSRYTGLQEKKGFLINIQPSELVDEQTKVIISVVDYFFISDMDERFKISYPFRPYFYIATLDGFEFQVSSYLSKKYGAQTAVEHMDREDLDLKDHLSGLKKTYIKLSFTSTVELIKIRKELMPLVRKNTDRIKKESAYADYLARNLSGKGGDSKDQQLNGDILNQIVDIREYDVPFHMRVSIDEKIFVGLWYDVKGIGPNRVPTIKRKDLPLFHAKPKVLAFDIETTKLPLKFPDRESDEIMMISYMVDGRGFLIINREIVSADINAFEYTPKAEYIGEFTVWNEKDEAALIRKFFDHFLQVRPNIVVTYNGDFFDWPFVEARAKIRGFNMEREIGFSKDSADEYKSRNCIHMDAFRWVKRDSYLPVGSQNLKAVTKAKLRYDPVEVEPELMCKMAREQPQQLANYSVSDAVSTYYLYMKYVHQFIFALCTIIPLGADDVLRKGSGTLCEALLMVEAFHNNIVFPNKYTGPEETRFSKDGHRVESETYVGGHVEALEAGVFRADIPAKFRLSVPALEQLKSEIQETLRKELAREFEVTLDQVVDFDEQCAEVQDAFDGMINVPTRLENPRIYHLDVGAMYPNIILTNRLQPCAMVTEEICMGCSYNKPDAECKRTMAWEWRGELTPATRGEYQQIMQQLEAESFGKPPKHFHMLERSEREAIEMKRIKDYSRRVYGKTHLTRLEMRETTICQRENHFYVETVKAFRDRRYEYKDMLKKAKGRFDQAQATNDLATMTTSKLEMVLYESLQLAHKCILNSFYGYVMRKGSRWFSMEMAGIVCHTGANIIREARKLVEQIGTPLELDTDGIWCLIPASFPENVTFKLKNHKRSSVTVSYPGAMLNALVYEGFTNHQYHTLEKDGSYSKSSENSIYFEVDGPYQCMILPASKEEGKKLKKRYAVFNLDGSLAEMKGFELKRRGELNIIKHFQGCVFKTFLNGKTLEETYKAVAADADHWLDILHSHGADLTDEELFDLISENRSMSRKLEDYGAQKSTSISTAKRLAEFLGDDMVKDAGLACMFIISKHPIGAPVTERAIPVAIFKSDAKVRSHYIRKWTKQVDFNEDTDIRDMLDWDYYLERFGSCIQKIITIPAALQGISNPVPRVPHPDWLQNKIRNKFDAHRQPRINQIFAACQKPSTSQMDNGKRRRTPDDDVASEDAMDSQDDIIIDDDKENGAKRQKNTKKVHTTEVVLEKKTLVEHGFDEWMGFLKKKWRVQRKERKTQLSSKDSDVVEAIVRGAREAEHDKEWHILSVEPTADASFFNVWLAVQGQMHKVIMKIGRRIIVDSRAPRGDRDTIRRILPHHKTPGFLYEFRTDENQLTALMDKLYSETCSSTIDGIYESEVPTSFRAVLQLGSIVRPDHGISLGGHQLTLENLKPMEKAPYLPLDQKIRTIFLYKFSQDSRHVYSLIDSSGSAAYFYIVNTGDVQMPNMDSLYTSAYTKMMSTERGQLCHTSESMPFTVKRFSSNTECERQLGRALRVYREVSSKTAIVLLLSDTDPFRLARKLPNLGLFPNVQLHITEPSSLLNQIDWQKVVARRVLQHYFNSFFFLADYLEWARYLRVPIGNLPADHALFGLDLLFARNLQKSGHALWATRASRPDLGGKEMDDVRLSVDWNPLSIDDTVLLNRETFCETACVELQLSAVAVTALVQRSRVLEAEGADDVVTFDSMNTIAQQSVTGGAVNSIACYDEGAAVDATIKVLKQMLTECVRHIAHQGNARADEVVMTVSRWLNTRSALLFDAALTRSVSVLESKLVLLLCAECERIGAKVIHATAQKLVLNTGKSTSEEAKGFAEMLIQSLSTNVVFAALHITPVKFFDAMLWMDAHNHTGIRISEKTESSPDVIADEEESSCTEFETTAIWKIAEEMPTEANIQEEFLQMIGAYILEFLETNRKMHFDSESGATFRSDCISQKISHRLYRIVNKMVHNNADIAHCSVYLANALCRALSCDQTSQLAVEGIRDNAKRLLHNSVVEADMTPLRSTTLFVSNVFCNSCSQASNVFLSSTDEILTCATCQSKLNSDVIDMMICDRLNQLLTAYQIQDHQCTKCKSVRHDTLSMYCECCSQFIPQITPAQLKHEASTVETVSIVRNFALSSELATWVLKML

Target ORF information:

RefSeq Version	NM_061215.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA polymerase epsilon catalytic subunit (pole-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_061215.3

ORF Insert Sequence:

ATGAGCTCCA AGGATGACAT TCTTGCACAG GCGGTCGAAA ATGATTCAAA CTACAAAGAG 
CGGCTTGCCC TGATCAGATC AAATGATGAG ATTGATGCGA AGCTTGGTTT CTCTAGATAC 
ACAGGACTAC AGGAGAAAAA AGGATTTCTG ATAAACATCC AACCTTCGGA GCTTGTCGAC 
GAGCAAACCA AAGTTATTAT TTCGGTCGTT GACTATTTCT TCATCAGTGA TATGGACGAA 
CGCTTCAAAA TCAGTTACCC ATTTCGACCG TATTTCTATA TTGCCACGTT GGATGGATTT 
GAATTCCAAG TATCCAGTTA TCTGTCCAAG AAGTACGGAG CCCAGACAGC AGTTGAACAT 
ATGGATCGGG AGGATTTAGA TTTGAAAGAT CATCTTTCTG GGTTGAAGAA GACATATATC 
AAACTCTCAT TCACCAGCAC CGTGGAATTG ATAAAAATCC GTAAGGAATT AATGCCGCTG 
GTGCGGAAAA ACACAGATCG CATCAAAAAA GAGTCGGCGT ACGCTGACTA TTTGGCGAGG 
AATCTGAGTG GAAAAGGAGG AGATAGTAAG GATCAACAGT TGAATGGTGA CATCTTGAAT 
CAAATTGTTG ATATCAGAGA ATACGACGTG CCTTTTCACA TGAGAGTTTC CATCGATGAA 
AAGATCTTCG TCGGATTATG GTACGATGTG AAAGGAATTG GCCCGAACCG TGTTCCAACA 
ATAAAGCGAA AAGATTTGCC CTTGTTCCAC GCAAAGCCGA AAGTTCTCGC CTTTGACATT 
GAAACAACGA AACTTCCGTT GAAATTTCCG GATCGTGAGA GCGATGAAAT TATGATGATA 
TCATATATGG TTGATGGACG TGGATTTTTG ATTATCAACC GAGAAATCGT GTCGGCTGAT 
ATTAATGCGT TCGAATACAC ACCGAAAGCC GAGTACATAG GAGAATTCAC AGTCTGGAAT 
GAGAAGGACG AAGCTGCTCT GATTCGGAAA TTTTTCGATC ATTTTTTGCA GGTTCGTCCA 
AATATTGTTG TCACCTACAA TGGAGATTTC TTCGATTGGC CATTCGTAGA AGCTCGTGCC 
AAGATCCGCG GATTCAATAT GGAAAGGGAA ATCGGATTTT CTAAGGATTC CGCCGATGAG 
TACAAATCAC GAAATTGTAT TCATATGGAC GCTTTCCGAT GGGTCAAACG TGACTCATAT 
CTACCGGTTG GAAGTCAGAA TCTGAAGGCA GTGACGAAGG CAAAGCTGAG ATATGATCCA 
GTTGAGGTGG AGCCGGAACT GATGTGCAAA ATGGCTCGAG AGCAGCCTCA ACAGCTGGCC 
AATTACTCGG TTTCGGATGC AGTGTCCACT TATTATCTCT ACATGAAGTA TGTTCATCAA 
TTTATCTTCG CTTTGTGCAC AATTATCCCA CTAGGAGCAG ATGACGTGTT GAGAAAAGGA 
TCTGGAACAC TTTGTGAAGC TCTTCTGATG GTTGAAGCAT TCCATAACAA TATTGTGTTC 
CCGAACAAGT ACACTGGACC AGAGGAGACA AGATTCAGTA AAGATGGACA TCGTGTTGAG 
AGCGAGACCT ATGTCGGAGG ACACGTGGAA GCTCTGGAGG CCGGCGTATT CAGAGCGGAT 
ATTCCAGCAA AATTCCGGCT TTCCGTTCCT GCTCTTGAGC AATTAAAATC CGAGATTCAA 
GAGACCCTTC GCAAGGAGCT GGCTCGAGAG TTTGAGGTCA CTCTTGACCA GGTCGTTGAC 
TTTGATGAGC AGTGCGCTGA AGTTCAAGAT GCATTTGATG GAATGATAAA TGTGCCAACA 
CGCCTCGAGA ATCCACGTAT CTACCATTTG GACGTCGGGG CCATGTACCC CAACATCATT 
TTGACTAATC GATTGCAGCC ATGTGCGATG GTCACCGAGG AGATTTGCAT GGGATGCTCC 
TACAACAAAC CAGACGCAGA ATGCAAGAGG ACGATGGCTT GGGAATGGAG GGGAGAGCTG 
ACACCAGCCA CTCGAGGAGA ATACCAGCAA ATTATGCAGC AACTCGAAGC AGAATCGTTT 
GGAAAACCAC CGAAACACTT TCATATGCTC GAGAGATCGG AGCGAGAAGC CATCGAAATG 
AAGCGTATCA AAGATTACAG CAGGCGTGTC TACGGAAAGA CTCATTTGAC AAGATTAGAA 
ATGAGAGAAA CTACAATTTG TCAACGGGAG AATCATTTCT ACGTGGAAAC TGTAAAAGCG 
TTCCGTGATA GAAGATACGA GTACAAGGAT ATGTTGAAAA AGGCGAAAGG ACGATTCGAT 
CAAGCCCAGG CGACAAATGA TCTTGCAACT ATGACTACAT CTAAGTTGGA AATGGTGCTC 
TACGAATCAC TGCAGCTGGC TCACAAGTGC ATTCTCAACT CTTTCTACGG ATATGTGATG 
CGTAAAGGAT CCAGATGGTT TTCAATGGAG ATGGCTGGAA TTGTGTGCCA TACTGGTGCA 
AATATCATCC GAGAAGCACG CAAACTTGTT GAACAGATTG GAACACCGTT GGAGCTTGAC 
ACTGATGGAA TTTGGTGCCT GATCCCTGCT TCGTTCCCTG AAAATGTCAC TTTCAAGCTG 
AAAAATCACA AAAGAAGTTC TGTTACAGTG TCGTATCCGG GTGCAATGCT GAATGCGTTG 
GTGTATGAAG GATTCACCAA TCATCAGTAC CACACTCTGG AAAAAGATGG GTCTTACTCA 
AAATCGTCGG AGAATTCGAT TTATTTCGAA GTAGATGGTC CATATCAATG TATGATTCTG 
CCTGCATCAA AGGAAGAGGG AAAGAAATTG AAGAAACGAT ACGCCGTGTT CAATTTAGAT 
GGATCACTGG CGGAAATGAA GGGATTCGAG TTGAAGAGAC GTGGAGAGTT GAATATTATC 
AAACATTTTC AGGGATGCGT ATTCAAAACC TTCCTCAACG GAAAAACTCT TGAGGAGACG 
TACAAAGCAG TGGCAGCTGA TGCGGATCAC TGGCTGGATA TTCTTCACTC CCATGGCGCC 
GATCTTACCG ATGAAGAACT ATTCGACTTG ATTTCAGAGA ACAGAAGTAT GTCGAGAAAG 
CTGGAAGACT ATGGAGCACA AAAGAGCACA TCAATCAGTA CTGCAAAACG GCTAGCTGAA 
TTCCTCGGAG ATGATATGGT CAAAGATGCT GGACTAGCCT GCATGTTCAT CATCTCGAAG 
CATCCGATTG GTGCACCGGT TACGGAAAGA GCTATTCCAG TTGCAATCTT CAAGTCAGAT 
GCTAAAGTTC GCAGTCATTA CATCAGGAAG TGGACGAAAC AAGTGGATTT CAATGAGGAT 
ACCGATATTC GGGATATGTT GGATTGGGAT TACTATTTGG AACGATTTGG CAGTTGTATT 
CAGAAGATTA TCACAATTCC AGCCGCACTT CAAGGCATAT CAAATCCAGT GCCACGTGTA 
CCACATCCAG ATTGGTTGCA GAACAAGATA CGGAATAAAT TCGACGCTCA CAGGCAGCCA 
CGTATCAATC AGATTTTCGC CGCCTGCCAA AAACCTTCAA CTTCACAAAT GGACAATGGA 
AAACGTCGTA GAACACCTGA TGATGACGTG GCATCTGAAG ATGCTATGGA TTCACAAGAT 
GACATCATAA TCGACGATGA TAAGGAGAAT GGAGCGAAGC GGCAGAAGAA CACCAAGAAG 
GTACATACAA CTGAGGTAGT CCTCGAGAAG AAGACGTTGG TGGAACACGG TTTCGATGAA 
TGGATGGGAT TTCTGAAAAA GAAGTGGAGA GTGCAGAGAA AGGAGAGAAA AACTCAGCTA 
TCATCGAAAG ATTCAGATGT AGTGGAGGCG ATTGTTCGTG GTGCGAGAGA AGCCGAGCAT 
GACAAGGAAT GGCATATTTT GAGTGTGGAG CCGACTGCGG ACGCCTCATT TTTCAATGTT 
TGGCTAGCAG TTCAGGGACA AATGCACAAA GTGATTATGA AGATTGGTAG ACGGATCATC 
GTCGATTCTC GTGCTCCACG CGGCGATCGT GATACAATTC GACGAATTTT ACCACATCAC 
AAGACACCTG GTTTCCTTTA CGAATTCCGT ACAGATGAGA ATCAGCTGAC AGCACTCATG 
GATAAGCTGT ATTCGGAGAC GTGTAGTTCG ACGATTGATG GAATCTACGA GTCAGAAGTT 
CCCACAAGCT TCCGTGCTGT ACTCCAACTT GGCTCAATCG TTCGACCGGA TCATGGAATC 
TCGCTGGGTG GACATCAACT GACACTGGAA AATTTAAAAC CTATGGAGAA GGCACCGTAT 
CTACCACTAG ATCAGAAGAT TCGGACTATC TTCTTGTATA AGTTCTCACA GGATTCTCGG 
CATGTCTATT CGCTTATTGA TTCGTCTGGC TCCGCAGCTT ACTTCTACAT TGTTAATACT 
GGAGATGTTC AGATGCCTAA TATGGACTCC CTCTACACAT CCGCATACAC TAAAATGATG 
TCAACTGAAC GTGGTCAGCT CTGTCACACT TCTGAAAGCA TGCCGTTCAC AGTGAAGCGA 
TTCTCATCAA ATACAGAATG CGAACGTCAA TTGGGAAGAG CATTGCGTGT ATATCGAGAG 
GTTTCGTCCA AAACTGCGAT TGTTCTTTTG CTCAGTGACA CGGATCCATT CCGATTGGCA 
CGAAAACTTC CGAATCTCGG ATTATTCCCA AATGTGCAGC TTCATATCAC TGAACCATCG 
TCACTTCTCA ATCAGATAGA CTGGCAAAAA GTCGTGGCTC GTCGAGTACT GCAACACTAT 
TTCAACAGCT TCTTCTTCCT CGCCGACTAT CTTGAATGGG CTCGCTATCT TCGTGTTCCA 
ATCGGTAATC TGCCTGCTGA TCATGCGCTC TTCGGATTGG ATCTTCTCTT CGCCAGAAAC 
CTCCAGAAAT CAGGTCACGC TCTGTGGGCA ACTCGTGCGA GCCGTCCGGA CTTGGGAGGA 
AAGGAAATGG ATGATGTTCG TCTTTCAGTT GATTGGAATC CGTTATCAAT AGATGATACG 
GTTCTGTTGA ATCGGGAGAC GTTCTGTGAA ACTGCCTGTG TTGAACTTCA ACTCAGCGCT 
GTTGCCGTGA CGGCTTTAGT TCAACGAAGT CGTGTTTTGG AGGCAGAAGG GGCCGACGAT 
GTAGTCACAT TTGACTCGAT GAATACAATT GCACAACAGT CAGTGACAGG AGGAGCTGTA 
AACTCAATCG CCTGCTATGA TGAAGGAGCC GCGGTGGATG CAACGATAAA AGTGCTCAAA 
CAAATGCTCA CAGAATGCGT TCGCCATATT GCCCATCAAG GAAATGCACG TGCCGATGAA 
GTTGTGATGA CCGTATCCAG ATGGCTCAAC ACTCGATCTG CTCTACTCTT TGACGCTGCC 
CTCACCAGGA GTGTTTCGGT GCTGGAGAGT AAGTTGGTGC TACTATTGTG TGCGGAATGT 
GAACGAATTG GAGCAAAAGT TATTCACGCA ACTGCTCAAA AGCTGGTGTT GAATACAGGA 
AAATCGACAA GCGAGGAGGC CAAGGGGTTT GCGGAGATGC TCATTCAGTC GTTGAGCACA 
AATGTTGTTT TTGCGGCTCT TCATATCACT CCTGTGAAGT TTTTCGATGC GATGCTCTGG 
ATGGACGCAC ACAATCATAC TGGAATTCGA ATTTCCGAAA AGACCGAATC GTCCCCAGAT 
GTGATAGCTG ATGAAGAAGA AAGTAGCTGC ACCGAGTTCG AGACAACTGC TATTTGGAAG 
ATCGCAGAAG AAATGCCAAC AGAGGCGAAT ATTCAGGAGG AATTCCTCCA AATGATTGGC 
GCCTACATTC TTGAATTCCT TGAAACCAAT CGAAAAATGC ACTTTGACTC GGAGTCCGGT 
GCAACTTTCC GGTCAGACTG CATCTCACAA AAGATCTCAC ATCGTCTCTA TCGAATTGTG 
AACAAGATGG TGCATAACAA TGCGGATATT GCACATTGTT CTGTGTATTT GGCGAATGCG 
CTCTGTCGAG CTCTATCCTG TGATCAGACC TCCCAGTTGG CAGTCGAAGG AATTCGGGAT 
AATGCGAAGC GTCTTCTGCA CAATTCTGTT GTGGAAGCCG ACATGACACC ACTGCGTTCA 
ACGACTCTCT TCGTCTCAAA TGTCTTCTGC AATTCGTGTT CTCAGGCCTC GAACGTATTC 
CTTTCCTCCA CTGATGAGAT TCTCACATGT GCCACGTGTC AGAGTAAGCT CAACTCTGAC 
GTCATTGATA TGATGATTTG CGATCGATTG AACCAGCTGC TCACTGCCTA CCAGATTCAG 
GATCATCAGT GCACGAAATG CAAATCGGTT CGTCACGACA CACTATCAAT GTACTGCGAA 
TGCTGTTCCC AGTTCATTCC ACAAATCACA CCTGCACAGC TGAAGCACGA GGCTTCCACC 
GTCGAGACTG TCTCCATCGT CCGCAATTTC GCGTTGAGCT CCGAACTCGC CACATGGGTC 
TTGAAAATGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pole-1 ( NM_061215.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_493616.1 Caenorhabditis elegans DNA polymerase epsilon catalytic subunit (pole-1), mRNA.

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CloneID	OCa335775
Clone ID Related Accession (Same CDS sequence)	NM_061215.3 , NM_061215.4
Accession Version	NM_061215.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	DNA polymerase epsilon catalytic subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003279). On Sep 4, 2019 this sequence version replaced NM_061215.3.

ATGAGCTCCA AGGATGACAT TCTTGCACAG GCGGTCGAAA ATGATTCAAA CTACAAAGAG 
CGGCTTGCCC TGATCAGATC AAATGATGAG ATTGATGCGA AGCTTGGTTT CTCTAGATAC 
ACAGGACTAC AGGAGAAAAA AGGATTTCTG ATAAACATCC AACCTTCGGA GCTTGTCGAC 
GAGCAAACCA AAGTTATTAT TTCGGTCGTT GACTATTTCT TCATCAGTGA TATGGACGAA 
CGCTTCAAAA TCAGTTACCC ATTTCGACCG TATTTCTATA TTGCCACGTT GGATGGATTT 
GAATTCCAAG TATCCAGTTA TCTGTCCAAG AAGTACGGAG CCCAGACAGC AGTTGAACAT 
ATGGATCGGG AGGATTTAGA TTTGAAAGAT CATCTTTCTG GGTTGAAGAA GACATATATC 
AAACTCTCAT TCACCAGCAC CGTGGAATTG ATAAAAATCC GTAAGGAATT AATGCCGCTG 
GTGCGGAAAA ACACAGATCG CATCAAAAAA GAGTCGGCGT ACGCTGACTA TTTGGCGAGG 
AATCTGAGTG GAAAAGGAGG AGATAGTAAG GATCAACAGT TGAATGGTGA CATCTTGAAT 
CAAATTGTTG ATATCAGAGA ATACGACGTG CCTTTTCACA TGAGAGTTTC CATCGATGAA 
AAGATCTTCG TCGGATTATG GTACGATGTG AAAGGAATTG GCCCGAACCG TGTTCCAACA 
ATAAAGCGAA AAGATTTGCC CTTGTTCCAC GCAAAGCCGA AAGTTCTCGC CTTTGACATT 
GAAACAACGA AACTTCCGTT GAAATTTCCG GATCGTGAGA GCGATGAAAT TATGATGATA 
TCATATATGG TTGATGGACG TGGATTTTTG ATTATCAACC GAGAAATCGT GTCGGCTGAT 
ATTAATGCGT TCGAATACAC ACCGAAAGCC GAGTACATAG GAGAATTCAC AGTCTGGAAT 
GAGAAGGACG AAGCTGCTCT GATTCGGAAA TTTTTCGATC ATTTTTTGCA GGTTCGTCCA 
AATATTGTTG TCACCTACAA TGGAGATTTC TTCGATTGGC CATTCGTAGA AGCTCGTGCC 
AAGATCCGCG GATTCAATAT GGAAAGGGAA ATCGGATTTT CTAAGGATTC CGCCGATGAG 
TACAAATCAC GAAATTGTAT TCATATGGAC GCTTTCCGAT GGGTCAAACG TGACTCATAT 
CTACCGGTTG GAAGTCAGAA TCTGAAGGCA GTGACGAAGG CAAAGCTGAG ATATGATCCA 
GTTGAGGTGG AGCCGGAACT GATGTGCAAA ATGGCTCGAG AGCAGCCTCA ACAGCTGGCC 
AATTACTCGG TTTCGGATGC AGTGTCCACT TATTATCTCT ACATGAAGTA TGTTCATCAA 
TTTATCTTCG CTTTGTGCAC AATTATCCCA CTAGGAGCAG ATGACGTGTT GAGAAAAGGA 
TCTGGAACAC TTTGTGAAGC TCTTCTGATG GTTGAAGCAT TCCATAACAA TATTGTGTTC 
CCGAACAAGT ACACTGGACC AGAGGAGACA AGATTCAGTA AAGATGGACA TCGTGTTGAG 
AGCGAGACCT ATGTCGGAGG ACACGTGGAA GCTCTGGAGG CCGGCGTATT CAGAGCGGAT 
ATTCCAGCAA AATTCCGGCT TTCCGTTCCT GCTCTTGAGC AATTAAAATC CGAGATTCAA 
GAGACCCTTC GCAAGGAGCT GGCTCGAGAG TTTGAGGTCA CTCTTGACCA GGTCGTTGAC 
TTTGATGAGC AGTGCGCTGA AGTTCAAGAT GCATTTGATG GAATGATAAA TGTGCCAACA 
CGCCTCGAGA ATCCACGTAT CTACCATTTG GACGTCGGGG CCATGTACCC CAACATCATT 
TTGACTAATC GATTGCAGCC ATGTGCGATG GTCACCGAGG AGATTTGCAT GGGATGCTCC 
TACAACAAAC CAGACGCAGA ATGCAAGAGG ACGATGGCTT GGGAATGGAG GGGAGAGCTG 
ACACCAGCCA CTCGAGGAGA ATACCAGCAA ATTATGCAGC AACTCGAAGC AGAATCGTTT 
GGAAAACCAC CGAAACACTT TCATATGCTC GAGAGATCGG AGCGAGAAGC CATCGAAATG 
AAGCGTATCA AAGATTACAG CAGGCGTGTC TACGGAAAGA CTCATTTGAC AAGATTAGAA 
ATGAGAGAAA CTACAATTTG TCAACGGGAG AATCATTTCT ACGTGGAAAC TGTAAAAGCG 
TTCCGTGATA GAAGATACGA GTACAAGGAT ATGTTGAAAA AGGCGAAAGG ACGATTCGAT 
CAAGCCCAGG CGACAAATGA TCTTGCAACT ATGACTACAT CTAAGTTGGA AATGGTGCTC 
TACGAATCAC TGCAGCTGGC TCACAAGTGC ATTCTCAACT CTTTCTACGG ATATGTGATG 
CGTAAAGGAT CCAGATGGTT TTCAATGGAG ATGGCTGGAA TTGTGTGCCA TACTGGTGCA 
AATATCATCC GAGAAGCACG CAAACTTGTT GAACAGATTG GAACACCGTT GGAGCTTGAC 
ACTGATGGAA TTTGGTGCCT GATCCCTGCT TCGTTCCCTG AAAATGTCAC TTTCAAGCTG 
AAAAATCACA AAAGAAGTTC TGTTACAGTG TCGTATCCGG GTGCAATGCT GAATGCGTTG 
GTGTATGAAG GATTCACCAA TCATCAGTAC CACACTCTGG AAAAAGATGG GTCTTACTCA 
AAATCGTCGG AGAATTCGAT TTATTTCGAA GTAGATGGTC CATATCAATG TATGATTCTG 
CCTGCATCAA AGGAAGAGGG AAAGAAATTG AAGAAACGAT ACGCCGTGTT CAATTTAGAT 
GGATCACTGG CGGAAATGAA GGGATTCGAG TTGAAGAGAC GTGGAGAGTT GAATATTATC 
AAACATTTTC AGGGATGCGT ATTCAAAACC TTCCTCAACG GAAAAACTCT TGAGGAGACG 
TACAAAGCAG TGGCAGCTGA TGCGGATCAC TGGCTGGATA TTCTTCACTC CCATGGCGCC 
GATCTTACCG ATGAAGAACT ATTCGACTTG ATTTCAGAGA ACAGAAGTAT GTCGAGAAAG 
CTGGAAGACT ATGGAGCACA AAAGAGCACA TCAATCAGTA CTGCAAAACG GCTAGCTGAA 
TTCCTCGGAG ATGATATGGT CAAAGATGCT GGACTAGCCT GCATGTTCAT CATCTCGAAG 
CATCCGATTG GTGCACCGGT TACGGAAAGA GCTATTCCAG TTGCAATCTT CAAGTCAGAT 
GCTAAAGTTC GCAGTCATTA CATCAGGAAG TGGACGAAAC AAGTGGATTT CAATGAGGAT 
ACCGATATTC GGGATATGTT GGATTGGGAT TACTATTTGG AACGATTTGG CAGTTGTATT 
CAGAAGATTA TCACAATTCC AGCCGCACTT CAAGGCATAT CAAATCCAGT GCCACGTGTA 
CCACATCCAG ATTGGTTGCA GAACAAGATA CGGAATAAAT TCGACGCTCA CAGGCAGCCA 
CGTATCAATC AGATTTTCGC CGCCTGCCAA AAACCTTCAA CTTCACAAAT GGACAATGGA 
AAACGTCGTA GAACACCTGA TGATGACGTG GCATCTGAAG ATGCTATGGA TTCACAAGAT 
GACATCATAA TCGACGATGA TAAGGAGAAT GGAGCGAAGC GGCAGAAGAA CACCAAGAAG 
GTACATACAA CTGAGGTAGT CCTCGAGAAG AAGACGTTGG TGGAACACGG TTTCGATGAA 
TGGATGGGAT TTCTGAAAAA GAAGTGGAGA GTGCAGAGAA AGGAGAGAAA AACTCAGCTA 
TCATCGAAAG ATTCAGATGT AGTGGAGGCG ATTGTTCGTG GTGCGAGAGA AGCCGAGCAT 
GACAAGGAAT GGCATATTTT GAGTGTGGAG CCGACTGCGG ACGCCTCATT TTTCAATGTT 
TGGCTAGCAG TTCAGGGACA AATGCACAAA GTGATTATGA AGATTGGTAG ACGGATCATC 
GTCGATTCTC GTGCTCCACG CGGCGATCGT GATACAATTC GACGAATTTT ACCACATCAC 
AAGACACCTG GTTTCCTTTA CGAATTCCGT ACAGATGAGA ATCAGCTGAC AGCACTCATG 
GATAAGCTGT ATTCGGAGAC GTGTAGTTCG ACGATTGATG GAATCTACGA GTCAGAAGTT 
CCCACAAGCT TCCGTGCTGT ACTCCAACTT GGCTCAATCG TTCGACCGGA TCATGGAATC 
TCGCTGGGTG GACATCAACT GACACTGGAA AATTTAAAAC CTATGGAGAA GGCACCGTAT 
CTACCACTAG ATCAGAAGAT TCGGACTATC TTCTTGTATA AGTTCTCACA GGATTCTCGG 
CATGTCTATT CGCTTATTGA TTCGTCTGGC TCCGCAGCTT ACTTCTACAT TGTTAATACT 
GGAGATGTTC AGATGCCTAA TATGGACTCC CTCTACACAT CCGCATACAC TAAAATGATG 
TCAACTGAAC GTGGTCAGCT CTGTCACACT TCTGAAAGCA TGCCGTTCAC AGTGAAGCGA 
TTCTCATCAA ATACAGAATG CGAACGTCAA TTGGGAAGAG CATTGCGTGT ATATCGAGAG 
GTTTCGTCCA AAACTGCGAT TGTTCTTTTG CTCAGTGACA CGGATCCATT CCGATTGGCA 
CGAAAACTTC CGAATCTCGG ATTATTCCCA AATGTGCAGC TTCATATCAC TGAACCATCG 
TCACTTCTCA ATCAGATAGA CTGGCAAAAA GTCGTGGCTC GTCGAGTACT GCAACACTAT 
TTCAACAGCT TCTTCTTCCT CGCCGACTAT CTTGAATGGG CTCGCTATCT TCGTGTTCCA 
ATCGGTAATC TGCCTGCTGA TCATGCGCTC TTCGGATTGG ATCTTCTCTT CGCCAGAAAC 
CTCCAGAAAT CAGGTCACGC TCTGTGGGCA ACTCGTGCGA GCCGTCCGGA CTTGGGAGGA 
AAGGAAATGG ATGATGTTCG TCTTTCAGTT GATTGGAATC CGTTATCAAT AGATGATACG 
GTTCTGTTGA ATCGGGAGAC GTTCTGTGAA ACTGCCTGTG TTGAACTTCA ACTCAGCGCT 
GTTGCCGTGA CGGCTTTAGT TCAACGAAGT CGTGTTTTGG AGGCAGAAGG GGCCGACGAT 
GTAGTCACAT TTGACTCGAT GAATACAATT GCACAACAGT CAGTGACAGG AGGAGCTGTA 
AACTCAATCG CCTGCTATGA TGAAGGAGCC GCGGTGGATG CAACGATAAA AGTGCTCAAA 
CAAATGCTCA CAGAATGCGT TCGCCATATT GCCCATCAAG GAAATGCACG TGCCGATGAA 
GTTGTGATGA CCGTATCCAG ATGGCTCAAC ACTCGATCTG CTCTACTCTT TGACGCTGCC 
CTCACCAGGA GTGTTTCGGT GCTGGAGAGT AAGTTGGTGC TACTATTGTG TGCGGAATGT 
GAACGAATTG GAGCAAAAGT TATTCACGCA ACTGCTCAAA AGCTGGTGTT GAATACAGGA 
AAATCGACAA GCGAGGAGGC CAAGGGGTTT GCGGAGATGC TCATTCAGTC GTTGAGCACA 
AATGTTGTTT TTGCGGCTCT TCATATCACT CCTGTGAAGT TTTTCGATGC GATGCTCTGG 
ATGGACGCAC ACAATCATAC TGGAATTCGA ATTTCCGAAA AGACCGAATC GTCCCCAGAT 
GTGATAGCTG ATGAAGAAGA AAGTAGCTGC ACCGAGTTCG AGACAACTGC TATTTGGAAG 
ATCGCAGAAG AAATGCCAAC AGAGGCGAAT ATTCAGGAGG AATTCCTCCA AATGATTGGC 
GCCTACATTC TTGAATTCCT TGAAACCAAT CGAAAAATGC ACTTTGACTC GGAGTCCGGT 
GCAACTTTCC GGTCAGACTG CATCTCACAA AAGATCTCAC ATCGTCTCTA TCGAATTGTG 
AACAAGATGG TGCATAACAA TGCGGATATT GCACATTGTT CTGTGTATTT GGCGAATGCG 
CTCTGTCGAG CTCTATCCTG TGATCAGACC TCCCAGTTGG CAGTCGAAGG AATTCGGGAT 
AATGCGAAGC GTCTTCTGCA CAATTCTGTT GTGGAAGCCG ACATGACACC ACTGCGTTCA 
ACGACTCTCT TCGTCTCAAA TGTCTTCTGC AATTCGTGTT CTCAGGCCTC GAACGTATTC 
CTTTCCTCCA CTGATGAGAT TCTCACATGT GCCACGTGTC AGAGTAAGCT CAACTCTGAC 
GTCATTGATA TGATGATTTG CGATCGATTG AACCAGCTGC TCACTGCCTA CCAGATTCAG 
GATCATCAGT GCACGAAATG CAAATCGGTT CGTCACGACA CACTATCAAT GTACTGCGAA 
TGCTGTTCCC AGTTCATTCC ACAAATCACA CCTGCACAGC TGAAGCACGA GGCTTCCACC 
GTCGAGACTG TCTCCATCGT CCGCAATTTC GCGTTGAGCT CCGAACTCGC CACATGGGTC 
TTGAAAATGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_493616.1
CDS	35..6469
Translation	MSSKDDILAQAVENDSNYKERLALIRSNDEIDAKLGFSRYTGLQEKKGFLINIQPSELVDEQTKVIISVVDYFFISDMDERFKISYPFRPYFYIATLDGFEFQVSSYLSKKYGAQTAVEHMDREDLDLKDHLSGLKKTYIKLSFTSTVELIKIRKELMPLVRKNTDRIKKESAYADYLARNLSGKGGDSKDQQLNGDILNQIVDIREYDVPFHMRVSIDEKIFVGLWYDVKGIGPNRVPTIKRKDLPLFHAKPKVLAFDIETTKLPLKFPDRESDEIMMISYMVDGRGFLIINREIVSADINAFEYTPKAEYIGEFTVWNEKDEAALIRKFFDHFLQVRPNIVVTYNGDFFDWPFVEARAKIRGFNMEREIGFSKDSADEYKSRNCIHMDAFRWVKRDSYLPVGSQNLKAVTKAKLRYDPVEVEPELMCKMAREQPQQLANYSVSDAVSTYYLYMKYVHQFIFALCTIIPLGADDVLRKGSGTLCEALLMVEAFHNNIVFPNKYTGPEETRFSKDGHRVESETYVGGHVEALEAGVFRADIPAKFRLSVPALEQLKSEIQETLRKELAREFEVTLDQVVDFDEQCAEVQDAFDGMINVPTRLENPRIYHLDVGAMYPNIILTNRLQPCAMVTEEICMGCSYNKPDAECKRTMAWEWRGELTPATRGEYQQIMQQLEAESFGKPPKHFHMLERSEREAIEMKRIKDYSRRVYGKTHLTRLEMRETTICQRENHFYVETVKAFRDRRYEYKDMLKKAKGRFDQAQATNDLATMTTSKLEMVLYESLQLAHKCILNSFYGYVMRKGSRWFSMEMAGIVCHTGANIIREARKLVEQIGTPLELDTDGIWCLIPASFPENVTFKLKNHKRSSVTVSYPGAMLNALVYEGFTNHQYHTLEKDGSYSKSSENSIYFEVDGPYQCMILPASKEEGKKLKKRYAVFNLDGSLAEMKGFELKRRGELNIIKHFQGCVFKTFLNGKTLEETYKAVAADADHWLDILHSHGADLTDEELFDLISENRSMSRKLEDYGAQKSTSISTAKRLAEFLGDDMVKDAGLACMFIISKHPIGAPVTERAIPVAIFKSDAKVRSHYIRKWTKQVDFNEDTDIRDMLDWDYYLERFGSCIQKIITIPAALQGISNPVPRVPHPDWLQNKIRNKFDAHRQPRINQIFAACQKPSTSQMDNGKRRRTPDDDVASEDAMDSQDDIIIDDDKENGAKRQKNTKKVHTTEVVLEKKTLVEHGFDEWMGFLKKKWRVQRKERKTQLSSKDSDVVEAIVRGAREAEHDKEWHILSVEPTADASFFNVWLAVQGQMHKVIMKIGRRIIVDSRAPRGDRDTIRRILPHHKTPGFLYEFRTDENQLTALMDKLYSETCSSTIDGIYESEVPTSFRAVLQLGSIVRPDHGISLGGHQLTLENLKPMEKAPYLPLDQKIRTIFLYKFSQDSRHVYSLIDSSGSAAYFYIVNTGDVQMPNMDSLYTSAYTKMMSTERGQLCHTSESMPFTVKRFSSNTECERQLGRALRVYREVSSKTAIVLLLSDTDPFRLARKLPNLGLFPNVQLHITEPSSLLNQIDWQKVVARRVLQHYFNSFFFLADYLEWARYLRVPIGNLPADHALFGLDLLFARNLQKSGHALWATRASRPDLGGKEMDDVRLSVDWNPLSIDDTVLLNRETFCETACVELQLSAVAVTALVQRSRVLEAEGADDVVTFDSMNTIAQQSVTGGAVNSIACYDEGAAVDATIKVLKQMLTECVRHIAHQGNARADEVVMTVSRWLNTRSALLFDAALTRSVSVLESKLVLLLCAECERIGAKVIHATAQKLVLNTGKSTSEEAKGFAEMLIQSLSTNVVFAALHITPVKFFDAMLWMDAHNHTGIRISEKTESSPDVIADEEESSCTEFETTAIWKIAEEMPTEANIQEEFLQMIGAYILEFLETNRKMHFDSESGATFRSDCISQKISHRLYRIVNKMVHNNADIAHCSVYLANALCRALSCDQTSQLAVEGIRDNAKRLLHNSVVEADMTPLRSTTLFVSNVFCNSCSQASNVFLSSTDEILTCATCQSKLNSDVIDMMICDRLNQLLTAYQIQDHQCTKCKSVRHDTLSMYCECCSQFIPQITPAQLKHEASTVETVSIVRNFALSSELATWVLKML

Target ORF information:

RefSeq Version	NM_061215.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans DNA polymerase epsilon catalytic subunit (pole-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_061215.4

ORF Insert Sequence:

ATGAGCTCCA AGGATGACAT TCTTGCACAG GCGGTCGAAA ATGATTCAAA CTACAAAGAG 
CGGCTTGCCC TGATCAGATC AAATGATGAG ATTGATGCGA AGCTTGGTTT CTCTAGATAC 
ACAGGACTAC AGGAGAAAAA AGGATTTCTG ATAAACATCC AACCTTCGGA GCTTGTCGAC 
GAGCAAACCA AAGTTATTAT TTCGGTCGTT GACTATTTCT TCATCAGTGA TATGGACGAA 
CGCTTCAAAA TCAGTTACCC ATTTCGACCG TATTTCTATA TTGCCACGTT GGATGGATTT 
GAATTCCAAG TATCCAGTTA TCTGTCCAAG AAGTACGGAG CCCAGACAGC AGTTGAACAT 
ATGGATCGGG AGGATTTAGA TTTGAAAGAT CATCTTTCTG GGTTGAAGAA GACATATATC 
AAACTCTCAT TCACCAGCAC CGTGGAATTG ATAAAAATCC GTAAGGAATT AATGCCGCTG 
GTGCGGAAAA ACACAGATCG CATCAAAAAA GAGTCGGCGT ACGCTGACTA TTTGGCGAGG 
AATCTGAGTG GAAAAGGAGG AGATAGTAAG GATCAACAGT TGAATGGTGA CATCTTGAAT 
CAAATTGTTG ATATCAGAGA ATACGACGTG CCTTTTCACA TGAGAGTTTC CATCGATGAA 
AAGATCTTCG TCGGATTATG GTACGATGTG AAAGGAATTG GCCCGAACCG TGTTCCAACA 
ATAAAGCGAA AAGATTTGCC CTTGTTCCAC GCAAAGCCGA AAGTTCTCGC CTTTGACATT 
GAAACAACGA AACTTCCGTT GAAATTTCCG GATCGTGAGA GCGATGAAAT TATGATGATA 
TCATATATGG TTGATGGACG TGGATTTTTG ATTATCAACC GAGAAATCGT GTCGGCTGAT 
ATTAATGCGT TCGAATACAC ACCGAAAGCC GAGTACATAG GAGAATTCAC AGTCTGGAAT 
GAGAAGGACG AAGCTGCTCT GATTCGGAAA TTTTTCGATC ATTTTTTGCA GGTTCGTCCA 
AATATTGTTG TCACCTACAA TGGAGATTTC TTCGATTGGC CATTCGTAGA AGCTCGTGCC 
AAGATCCGCG GATTCAATAT GGAAAGGGAA ATCGGATTTT CTAAGGATTC CGCCGATGAG 
TACAAATCAC GAAATTGTAT TCATATGGAC GCTTTCCGAT GGGTCAAACG TGACTCATAT 
CTACCGGTTG GAAGTCAGAA TCTGAAGGCA GTGACGAAGG CAAAGCTGAG ATATGATCCA 
GTTGAGGTGG AGCCGGAACT GATGTGCAAA ATGGCTCGAG AGCAGCCTCA ACAGCTGGCC 
AATTACTCGG TTTCGGATGC AGTGTCCACT TATTATCTCT ACATGAAGTA TGTTCATCAA 
TTTATCTTCG CTTTGTGCAC AATTATCCCA CTAGGAGCAG ATGACGTGTT GAGAAAAGGA 
TCTGGAACAC TTTGTGAAGC TCTTCTGATG GTTGAAGCAT TCCATAACAA TATTGTGTTC 
CCGAACAAGT ACACTGGACC AGAGGAGACA AGATTCAGTA AAGATGGACA TCGTGTTGAG 
AGCGAGACCT ATGTCGGAGG ACACGTGGAA GCTCTGGAGG CCGGCGTATT CAGAGCGGAT 
ATTCCAGCAA AATTCCGGCT TTCCGTTCCT GCTCTTGAGC AATTAAAATC CGAGATTCAA 
GAGACCCTTC GCAAGGAGCT GGCTCGAGAG TTTGAGGTCA CTCTTGACCA GGTCGTTGAC 
TTTGATGAGC AGTGCGCTGA AGTTCAAGAT GCATTTGATG GAATGATAAA TGTGCCAACA 
CGCCTCGAGA ATCCACGTAT CTACCATTTG GACGTCGGGG CCATGTACCC CAACATCATT 
TTGACTAATC GATTGCAGCC ATGTGCGATG GTCACCGAGG AGATTTGCAT GGGATGCTCC 
TACAACAAAC CAGACGCAGA ATGCAAGAGG ACGATGGCTT GGGAATGGAG GGGAGAGCTG 
ACACCAGCCA CTCGAGGAGA ATACCAGCAA ATTATGCAGC AACTCGAAGC AGAATCGTTT 
GGAAAACCAC CGAAACACTT TCATATGCTC GAGAGATCGG AGCGAGAAGC CATCGAAATG 
AAGCGTATCA AAGATTACAG CAGGCGTGTC TACGGAAAGA CTCATTTGAC AAGATTAGAA 
ATGAGAGAAA CTACAATTTG TCAACGGGAG AATCATTTCT ACGTGGAAAC TGTAAAAGCG 
TTCCGTGATA GAAGATACGA GTACAAGGAT ATGTTGAAAA AGGCGAAAGG ACGATTCGAT 
CAAGCCCAGG CGACAAATGA TCTTGCAACT ATGACTACAT CTAAGTTGGA AATGGTGCTC 
TACGAATCAC TGCAGCTGGC TCACAAGTGC ATTCTCAACT CTTTCTACGG ATATGTGATG 
CGTAAAGGAT CCAGATGGTT TTCAATGGAG ATGGCTGGAA TTGTGTGCCA TACTGGTGCA 
AATATCATCC GAGAAGCACG CAAACTTGTT GAACAGATTG GAACACCGTT GGAGCTTGAC 
ACTGATGGAA TTTGGTGCCT GATCCCTGCT TCGTTCCCTG AAAATGTCAC TTTCAAGCTG 
AAAAATCACA AAAGAAGTTC TGTTACAGTG TCGTATCCGG GTGCAATGCT GAATGCGTTG 
GTGTATGAAG GATTCACCAA TCATCAGTAC CACACTCTGG AAAAAGATGG GTCTTACTCA 
AAATCGTCGG AGAATTCGAT TTATTTCGAA GTAGATGGTC CATATCAATG TATGATTCTG 
CCTGCATCAA AGGAAGAGGG AAAGAAATTG AAGAAACGAT ACGCCGTGTT CAATTTAGAT 
GGATCACTGG CGGAAATGAA GGGATTCGAG TTGAAGAGAC GTGGAGAGTT GAATATTATC 
AAACATTTTC AGGGATGCGT ATTCAAAACC TTCCTCAACG GAAAAACTCT TGAGGAGACG 
TACAAAGCAG TGGCAGCTGA TGCGGATCAC TGGCTGGATA TTCTTCACTC CCATGGCGCC 
GATCTTACCG ATGAAGAACT ATTCGACTTG ATTTCAGAGA ACAGAAGTAT GTCGAGAAAG 
CTGGAAGACT ATGGAGCACA AAAGAGCACA TCAATCAGTA CTGCAAAACG GCTAGCTGAA 
TTCCTCGGAG ATGATATGGT CAAAGATGCT GGACTAGCCT GCATGTTCAT CATCTCGAAG 
CATCCGATTG GTGCACCGGT TACGGAAAGA GCTATTCCAG TTGCAATCTT CAAGTCAGAT 
GCTAAAGTTC GCAGTCATTA CATCAGGAAG TGGACGAAAC AAGTGGATTT CAATGAGGAT 
ACCGATATTC GGGATATGTT GGATTGGGAT TACTATTTGG AACGATTTGG CAGTTGTATT 
CAGAAGATTA TCACAATTCC AGCCGCACTT CAAGGCATAT CAAATCCAGT GCCACGTGTA 
CCACATCCAG ATTGGTTGCA GAACAAGATA CGGAATAAAT TCGACGCTCA CAGGCAGCCA 
CGTATCAATC AGATTTTCGC CGCCTGCCAA AAACCTTCAA CTTCACAAAT GGACAATGGA 
AAACGTCGTA GAACACCTGA TGATGACGTG GCATCTGAAG ATGCTATGGA TTCACAAGAT 
GACATCATAA TCGACGATGA TAAGGAGAAT GGAGCGAAGC GGCAGAAGAA CACCAAGAAG 
GTACATACAA CTGAGGTAGT CCTCGAGAAG AAGACGTTGG TGGAACACGG TTTCGATGAA 
TGGATGGGAT TTCTGAAAAA GAAGTGGAGA GTGCAGAGAA AGGAGAGAAA AACTCAGCTA 
TCATCGAAAG ATTCAGATGT AGTGGAGGCG ATTGTTCGTG GTGCGAGAGA AGCCGAGCAT 
GACAAGGAAT GGCATATTTT GAGTGTGGAG CCGACTGCGG ACGCCTCATT TTTCAATGTT 
TGGCTAGCAG TTCAGGGACA AATGCACAAA GTGATTATGA AGATTGGTAG ACGGATCATC 
GTCGATTCTC GTGCTCCACG CGGCGATCGT GATACAATTC GACGAATTTT ACCACATCAC 
AAGACACCTG GTTTCCTTTA CGAATTCCGT ACAGATGAGA ATCAGCTGAC AGCACTCATG 
GATAAGCTGT ATTCGGAGAC GTGTAGTTCG ACGATTGATG GAATCTACGA GTCAGAAGTT 
CCCACAAGCT TCCGTGCTGT ACTCCAACTT GGCTCAATCG TTCGACCGGA TCATGGAATC 
TCGCTGGGTG GACATCAACT GACACTGGAA AATTTAAAAC CTATGGAGAA GGCACCGTAT 
CTACCACTAG ATCAGAAGAT TCGGACTATC TTCTTGTATA AGTTCTCACA GGATTCTCGG 
CATGTCTATT CGCTTATTGA TTCGTCTGGC TCCGCAGCTT ACTTCTACAT TGTTAATACT 
GGAGATGTTC AGATGCCTAA TATGGACTCC CTCTACACAT CCGCATACAC TAAAATGATG 
TCAACTGAAC GTGGTCAGCT CTGTCACACT TCTGAAAGCA TGCCGTTCAC AGTGAAGCGA 
TTCTCATCAA ATACAGAATG CGAACGTCAA TTGGGAAGAG CATTGCGTGT ATATCGAGAG 
GTTTCGTCCA AAACTGCGAT TGTTCTTTTG CTCAGTGACA CGGATCCATT CCGATTGGCA 
CGAAAACTTC CGAATCTCGG ATTATTCCCA AATGTGCAGC TTCATATCAC TGAACCATCG 
TCACTTCTCA ATCAGATAGA CTGGCAAAAA GTCGTGGCTC GTCGAGTACT GCAACACTAT 
TTCAACAGCT TCTTCTTCCT CGCCGACTAT CTTGAATGGG CTCGCTATCT TCGTGTTCCA 
ATCGGTAATC TGCCTGCTGA TCATGCGCTC TTCGGATTGG ATCTTCTCTT CGCCAGAAAC 
CTCCAGAAAT CAGGTCACGC TCTGTGGGCA ACTCGTGCGA GCCGTCCGGA CTTGGGAGGA 
AAGGAAATGG ATGATGTTCG TCTTTCAGTT GATTGGAATC CGTTATCAAT AGATGATACG 
GTTCTGTTGA ATCGGGAGAC GTTCTGTGAA ACTGCCTGTG TTGAACTTCA ACTCAGCGCT 
GTTGCCGTGA CGGCTTTAGT TCAACGAAGT CGTGTTTTGG AGGCAGAAGG GGCCGACGAT 
GTAGTCACAT TTGACTCGAT GAATACAATT GCACAACAGT CAGTGACAGG AGGAGCTGTA 
AACTCAATCG CCTGCTATGA TGAAGGAGCC GCGGTGGATG CAACGATAAA AGTGCTCAAA 
CAAATGCTCA CAGAATGCGT TCGCCATATT GCCCATCAAG GAAATGCACG TGCCGATGAA 
GTTGTGATGA CCGTATCCAG ATGGCTCAAC ACTCGATCTG CTCTACTCTT TGACGCTGCC 
CTCACCAGGA GTGTTTCGGT GCTGGAGAGT AAGTTGGTGC TACTATTGTG TGCGGAATGT 
GAACGAATTG GAGCAAAAGT TATTCACGCA ACTGCTCAAA AGCTGGTGTT GAATACAGGA 
AAATCGACAA GCGAGGAGGC CAAGGGGTTT GCGGAGATGC TCATTCAGTC GTTGAGCACA 
AATGTTGTTT TTGCGGCTCT TCATATCACT CCTGTGAAGT TTTTCGATGC GATGCTCTGG 
ATGGACGCAC ACAATCATAC TGGAATTCGA ATTTCCGAAA AGACCGAATC GTCCCCAGAT 
GTGATAGCTG ATGAAGAAGA AAGTAGCTGC ACCGAGTTCG AGACAACTGC TATTTGGAAG 
ATCGCAGAAG AAATGCCAAC AGAGGCGAAT ATTCAGGAGG AATTCCTCCA AATGATTGGC 
GCCTACATTC TTGAATTCCT TGAAACCAAT CGAAAAATGC ACTTTGACTC GGAGTCCGGT 
GCAACTTTCC GGTCAGACTG CATCTCACAA AAGATCTCAC ATCGTCTCTA TCGAATTGTG 
AACAAGATGG TGCATAACAA TGCGGATATT GCACATTGTT CTGTGTATTT GGCGAATGCG 
CTCTGTCGAG CTCTATCCTG TGATCAGACC TCCCAGTTGG CAGTCGAAGG AATTCGGGAT 
AATGCGAAGC GTCTTCTGCA CAATTCTGTT GTGGAAGCCG ACATGACACC ACTGCGTTCA 
ACGACTCTCT TCGTCTCAAA TGTCTTCTGC AATTCGTGTT CTCAGGCCTC GAACGTATTC 
CTTTCCTCCA CTGATGAGAT TCTCACATGT GCCACGTGTC AGAGTAAGCT CAACTCTGAC 
GTCATTGATA TGATGATTTG CGATCGATTG AACCAGCTGC TCACTGCCTA CCAGATTCAG 
GATCATCAGT GCACGAAATG CAAATCGGTT CGTCACGACA CACTATCAAT GTACTGCGAA 
TGCTGTTCCC AGTTCATTCC ACAAATCACA CCTGCACAGC TGAAGCACGA GGCTTCCACC 
GTCGAGACTG TCTCCATCGT CCGCAATTTC GCGTTGAGCT CCGAACTCGC CACATGGGTC 
TTGAAAATGT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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pole-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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