POLE gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011534802.3	XP_011533104.1	DNA polymerase epsilon catalytic subunit A isoform X4
XM_011534798.1	XP_011533100.1	DNA polymerase epsilon catalytic subunit A isoform X3
XM_011534797.3	XP_011533099.1	DNA polymerase epsilon catalytic subunit A isoform X2
XM_011534797.2	XP_011533099.1	DNA polymerase epsilon catalytic subunit A isoform X2
XM_011534800.2	XP_011533102.1	DNA polymerase epsilon catalytic subunit A isoform X5
XM_011534802.2	XP_011533104.1	DNA polymerase epsilon catalytic subunit A isoform X6
NM_006231.3	NP_006222.2	DNA polymerase epsilon catalytic subunit A
XM_011534799.2	XP_011533101.1	DNA polymerase epsilon catalytic subunit A isoform X3
NM_006231.4	NP_006222.2	DNA polymerase epsilon catalytic subunit A
XM_011534795.2	XP_011533097.1	DNA polymerase epsilon catalytic subunit A isoform X1
XM_011534795.3	XP_011533097.1	DNA polymerase epsilon catalytic subunit A isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu59815	XM_011534802.3 Latest version!	Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu59810	XM_011534797.3 Latest version!	Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu59811	XM_011534798.1 Latest version!	Homo sapiens polymerase (DNA) epsilon, catalytic subunit (POLE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu59812	XM_011534799.2 Latest version!	Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu59813	XM_011534800.2 Latest version!	Homo sapiens polymerase (DNA) epsilon, catalytic subunit (POLE), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu20900	NM_006231.4 Latest version!	Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu59808	XM_011534795.3 Latest version!	Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu59810	XM_011534797.2	Homo sapiens polymerase (DNA) epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu59815	XM_011534802.2	Homo sapiens polymerase (DNA) epsilon, catalytic subunit (POLE), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu20900	NM_006231.3	Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu59808	XM_011534795.2	Homo sapiens polymerase (DNA) epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

POLE ( XM_011534802.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533104.1 Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu59815
Clone ID Related Accession (Same CDS sequence)	XM_011534802.3 , XM_011534802.2
Accession Version	XM_011534802.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA polymerase epsilon catalytic subunit A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011534802.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCAAG AAGGGGACCC CGTGCTTCCT GGGCTCCTGG AGACCAAGGC AGCCAACATG 
CCTGACTCTG AGCTATTCGA GCTCATCTCT GAGAACCGTT CCATGTCTCG GAAGCTGGAA 
GATTACGGGG AGCAGAAGTC TACGTCCATC AGCACAGCAA AGCGCCTGGC CGAGTTCCTG 
GGAGACCAGA TGGTCAAGGA TGCAGGGCTG AGTTGCCGCT ACATCATCTC CCGCAAGCCC 
GAGGGCTCCC CTGTCACGGA GAGGGCCATC CCACTTGCCA TTTTCCAAGC AGAGCCCACG 
GTGAGGAAGC ACTTTCTCCG GAAATGGCTC AAGAGCTCTT CCCTTCAAGA CTTTGATATT 
CGAGCAATTC TGGATTGGGA CTACTACATT GAGCGGCTGG GAAGCGCCAT CCAGAAGATC 
ATCACCATCC CTGCGGCCCT GCAGCAGGTA AAGAACCCAG TGCCACGTGT CAAACACCCC 
GACTGGCTGC ACAAAAAACT GCTGGAGAAG AATGATGTCT ACAAGCAGAA GAAGATCAGT 
GAGCTCTTCA CCCTGGAGGG CAGGAGACAG GTCACGATGG CCGAGGCCTC AGAAGACAGT 
CCGAGGCCAA GTGCTCCTGA CATGGAGGAC TTCGGCCTCG TAAAGCTGCC TCACCCAGCA 
GCCCCTGTCA CTGTGAAGAG GAAGCGAGTT CTTTGGGAGA GCCAGGAGGA GTCCCAGGAC 
CTCACGCCGA CTGTGCCCTG GCAGGAAATC TTGGGGCAGC CTCCCGCCCT GGGAACCAGC 
CAGGAGGAAT GGCTTGTCTG GCTCCGGTTC CACAAGAAGA AGTGGCAGCT GCAGGCCCGG 
CAGCGCCTCG CCCGCAGGAA GAGGCAGCGT CTGGAGTCGG CAGAGGGTGT GCTCAGGCCC 
GGGGCCATCC GGGATGGTCC TGCCACGGGG CTGGGGAGCT TCTTGCGAAG AACTGCCCGC 
AGCATCCTGG ACCTTCCGTG GCAGATTGTG CAGATCAGCG AGACCAGCCA GGCCGGCCTG 
TTCAGGCTGT GGGCGCTCGT TGGCAGTGAC TTGCACTGCA TCAGGCTGAG CATCCCCCGT 
GTGTTCTACG TGAACCAGCG AGTCGCTAAA GCGGAGGAGG GTGCTTCGTA TCGCAAGGTA 
AATCGGGTCC TTCCTCGCTC CAACATGGTC TACAATCTCT ATGAGTATTC AGTGCCAGAG 
GACATGTACC AGGAACACAT CAACGAGATC AACGCTGAGC TGTCAGCGCC AGACATCGAG 
GGCGTATATG AGACTCAGGT TCCGTTACTG TTCCGGGCCC TGGTGCACCT GGGCTGTGTG 
TGTGTGGTCA ATAAACAGCT GGTGAGGCAC CTTTCAGGCT GGGAAGCAGA GACCTTTGCT 
CTTGAGCACC TGGAGATGCG CTCTCTGGCC CAGTTCAGCT ACCTGGAACC AGGGAGTATC 
CGCCATATCT ACCTGTACCA CCACGCACAG GCCCACAAAG CGCTCTTCGG GATCTTCATC 
CCCTCACAGC GCAGGGCATC CGTCTTTGTG CTGGACACTG TGCGCAGCAA CCAGATGCCC 
AGCCTTGGCG CCCTGTACTC AGCAGAGCAC GGCCTCCTCC TGGAGAAGGT GGGCCCTGAG 
CTCCTGCCAC CCCCCAAACA CACCTTCGAA GTTCGGGCAG AAACTGACCT GAAGACCATC 
TGCAGAGCCA TCCAGCGATT CCTGCTCGCC TACAAGGAGG AGCGCCGGGG GCCCACACTC 
ATCGCTGTTC AGTCCAGCTG GGAGCTGAAG AGGCTGGCCA GTGAAATTCC TGTCTTGGAG 
GAATTCCCAC TGGTGCCTAT CTGTGTGGCT GACAAGATCA ACTATGGGGT CCTGGACTGG 
CAGCGCCATG GAGCCCGGCG CATGATCCGT CACTACCTCA ACCTGGACAC CTGCCTGTCG 
CAGGCCTTCG AGATGAGCAG GTACTTTCAC ATTCCCATTG GGAACCTACC AGAGGACATC 
TCCACATTCG GCTCCGACCT CTTCTTTGCC CGCCACCTCC AGCGCCACAA CCACCTGCTC 
TGGCTGTCCC CTACAGCCCG CCCTGACCTG GGTGGAAAGG AGGCTGATGA CAACTGTCTT 
GTCATGGAGT TCGATGACCA AGCCACTGTT GAGATCAACA GTTCAGGCTG TTACTCCACA 
GTGTGTGTGG AGCTGGACCT TCAGAACCTG GCCGTCAACA CCATTCTCCA GTCTCACCAT 
GTCAACGACA TGGAGGGGGC CGACAGCATG GGGATCAGCT TCGACGTGAT CCAGCAGGCC 
TCCCTGGAGG ACATGATCAC GGGTGGTCAG GCTGCCAGTG CCCCGGCCAG CTACGATGAG 
ACAGCCCTGT GCTCTAACAC CTTCAGGATC CTGAAGAGCA TGGTCGTGGG CTGGGTGAAG 
GAGATCACCC AGTACCACAA CATCTATGCA GACAACCAGG TGATGCACTT CTACCGCTGG 
CTTCGGTCGC CATCCTCTCT GCTTCATGAC CCTGCCCTGC ACCGCACACT CCACAACATG 
ATGAAGAAGC TCTTCCTGCA GCTCATCGCT GAGTTCAAGC GCCTGGGGTC ATCAGTCATC 
TACGCCAACT TCAACCGCAT CATCCTCTGT ACAAAGAAGC GCCGTGTGGA AGATGCCATC 
GCTTACGTGG AGTACATCAC CAGCAGCATC CATTCAAAGG AGACCTTCCA TTCTCTGACA 
ATTTCTTTCT CTCGATGCTG GGAATTTCTT CTCTGGATGG ATCCATCTAA CTATGGCGGA 
ATCAAAGGAA AAGTTTCATC TCGTATTCAC TGTGGACTGC AAGACTCCCA GAAAGCAGGG 
GGAGCAGAGG ATGAGCAGGA AAATGAGGAC GATGAGGAGG AAAGAGATGG GGAGGAGGAG 
GAAGAGGCGG AGGAATCCAA CGTGGAGGAT TTACTGGAAA ACAACTGGAA CATTTTGCAG 
TTTTTGCCAC AGGCAGCCTC CTGCCAGAAC TACTTCCTCA TGATTGTTTC AGCGTACATC 
GTGGCCGTGT ACCACTGCAT GAAGGACGGG CTGAGGCGCA GTGCTCCAGG GAGCACCCCC 
GTGAGGAGGA GGGGGGCCAG CCAGCTCTCC CAGGAGGCCG AGGGGGCGGT CGGAGCCCTT 
CCCGGAATGA TCACCTTCTC TCAGGATTAT GTCGCAAATG AGCTCACTCA GAGCTTCTTC 
ACCATCACTC AGAAGATTCA GAAGAAAGTC ACAGGCTCTC GGAACTCCAC TGAGCTCTCA 
GAGATGTTTC CTGTCCTCCC CGGTTCCCAC TTGCTGCTCA ATAACCCTGC CCTGGAGTTC 
ATCAAATACG TGTGCAAGGT GCTGTCCCTG GACACCAACA TCACAAACCA GGTGAATAAG 
CTGAACCGAG ACCTGCTTCG CCTGGTGGAT GTCGGCGAGT TCTCCGAGGA GGCCCAGTTC 
CGAGACCCCT GCCGCTCCTA CGTGCTTCCT GAGGTCATCT GCCGCAGCTG TAACTTCTGC 
CGCGACCTGG ACCTGTGTAA AGACTCTTCC TTCTCAGAGG ATGGGGCGGT CCTGCCTCAG 
TGGCTCTGCT CCAACTGTCA GGCGCCCTAC GACTCCTCTG CCATCGAGAT GACGCTGGTG 
GAAGTTCTAC AGAAGAAGCT GATGGCCTTC ACCCTGCAGG ACCTGCGGAA GGAGGCAGAC 
ACTCCCAAGT GTGAAGAGAA GGAAAGGAGG AAGAGGGAGT TGCACAGCTC GGCCACAGGA 
AGGTCTTGCC GAGGGGCATC TGAGCAGAGG CTGGGGGACG CGCCAAGCTG CCGTGTGGTG 
GTGCACGGCG TTCAGGCCTG CAGGATGGAG GGAGTGAGCA GGGCCTGGAG GAGGCCTCAG 
GACTGGGCAT CTGGGGTGGA ACATCCACAG CGAGGGTGGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533104.1
CDS	39..3923
Translation	MKQEGDPVLPGLLETKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRAILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFTLEGRRQVTMAEASEDSPRPSAPDMEDFGLVKLPHPAAPVTVKRKRVLWESQEESQDLTPTVPWQEILGQPPALGTSQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEGVLRPGAIRDGPATGLGSFLRRTARSILDLPWQIVQISETSQAGLFRLWALVGSDLHCIRLSIPRVFYVNQRVAKAEEGASYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINAELSAPDIEGVYETQVPLLFRALVHLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHAQAHKALFGIFIPSQRRASVFVLDTVRSNQMPSLGALYSAEHGLLLEKVGPELLPPPKHTFEVRAETDLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELKRLASEIPVLEEFPLVPICVADKINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTARPDLGGKEADDNCLVMEFDDQATVEINSSGCYSTVCVELDLQNLAVNTILQSHHVNDMEGADSMGISFDVIQQASLEDMITGGQAASAPASYDETALCSNTFRILKSMVVGWVKEITQYHNIYADNQVMHFYRWLRSPSSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVIYANFNRIILCTKKRRVEDAIAYVEYITSSIHSKETFHSLTISFSRCWEFLLWMDPSNYGGIKGKVSSRIHCGLQDSQKAGGAEDEQENEDDEEERDGEEEEEAEESNVEDLLENNWNILQFLPQAASCQNYFLMIVSAYIVAVYHCMKDGLRRSAPGSTPVRRRGASQLSQEAEGAVGALPGMITFSQDYVANELTQSFFTITQKIQKKVTGSRNSTELSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCKDSSFSEDGAVLPQWLCSNCQAPYDSSAIEMTLVEVLQKKLMAFTLQDLRKEADTPKCEEKERRKRELHSSATGRSCRGASEQRLGDAPSCRVVVHGVQACRMEGVSRAWRRPQDWASGVEHPQRGWV

Target ORF information:

RefSeq Version	XM_011534802.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534802.3

ORF Insert Sequence:

ATGAAGCAAG AAGGGGACCC CGTGCTTCCT GGGCTCCTGG AGACCAAGGC AGCCAACATG 
CCTGACTCTG AGCTATTCGA GCTCATCTCT GAGAACCGTT CCATGTCTCG GAAGCTGGAA 
GATTACGGGG AGCAGAAGTC TACGTCCATC AGCACAGCAA AGCGCCTGGC CGAGTTCCTG 
GGAGACCAGA TGGTCAAGGA TGCAGGGCTG AGTTGCCGCT ACATCATCTC CCGCAAGCCC 
GAGGGCTCCC CTGTCACGGA GAGGGCCATC CCACTTGCCA TTTTCCAAGC AGAGCCCACG 
GTGAGGAAGC ACTTTCTCCG GAAATGGCTC AAGAGCTCTT CCCTTCAAGA CTTTGATATT 
CGAGCAATTC TGGATTGGGA CTACTACATT GAGCGGCTGG GAAGCGCCAT CCAGAAGATC 
ATCACCATCC CTGCGGCCCT GCAGCAGGTA AAGAACCCAG TGCCACGTGT CAAACACCCC 
GACTGGCTGC ACAAAAAACT GCTGGAGAAG AATGATGTCT ACAAGCAGAA GAAGATCAGT 
GAGCTCTTCA CCCTGGAGGG CAGGAGACAG GTCACGATGG CCGAGGCCTC AGAAGACAGT 
CCGAGGCCAA GTGCTCCTGA CATGGAGGAC TTCGGCCTCG TAAAGCTGCC TCACCCAGCA 
GCCCCTGTCA CTGTGAAGAG GAAGCGAGTT CTTTGGGAGA GCCAGGAGGA GTCCCAGGAC 
CTCACGCCGA CTGTGCCCTG GCAGGAAATC TTGGGGCAGC CTCCCGCCCT GGGAACCAGC 
CAGGAGGAAT GGCTTGTCTG GCTCCGGTTC CACAAGAAGA AGTGGCAGCT GCAGGCCCGG 
CAGCGCCTCG CCCGCAGGAA GAGGCAGCGT CTGGAGTCGG CAGAGGGTGT GCTCAGGCCC 
GGGGCCATCC GGGATGGTCC TGCCACGGGG CTGGGGAGCT TCTTGCGAAG AACTGCCCGC 
AGCATCCTGG ACCTTCCGTG GCAGATTGTG CAGATCAGCG AGACCAGCCA GGCCGGCCTG 
TTCAGGCTGT GGGCGCTCGT TGGCAGTGAC TTGCACTGCA TCAGGCTGAG CATCCCCCGT 
GTGTTCTACG TGAACCAGCG AGTCGCTAAA GCGGAGGAGG GTGCTTCGTA TCGCAAGGTA 
AATCGGGTCC TTCCTCGCTC CAACATGGTC TACAATCTCT ATGAGTATTC AGTGCCAGAG 
GACATGTACC AGGAACACAT CAACGAGATC AACGCTGAGC TGTCAGCGCC AGACATCGAG 
GGCGTATATG AGACTCAGGT TCCGTTACTG TTCCGGGCCC TGGTGCACCT GGGCTGTGTG 
TGTGTGGTCA ATAAACAGCT GGTGAGGCAC CTTTCAGGCT GGGAAGCAGA GACCTTTGCT 
CTTGAGCACC TGGAGATGCG CTCTCTGGCC CAGTTCAGCT ACCTGGAACC AGGGAGTATC 
CGCCATATCT ACCTGTACCA CCACGCACAG GCCCACAAAG CGCTCTTCGG GATCTTCATC 
CCCTCACAGC GCAGGGCATC CGTCTTTGTG CTGGACACTG TGCGCAGCAA CCAGATGCCC 
AGCCTTGGCG CCCTGTACTC AGCAGAGCAC GGCCTCCTCC TGGAGAAGGT GGGCCCTGAG 
CTCCTGCCAC CCCCCAAACA CACCTTCGAA GTTCGGGCAG AAACTGACCT GAAGACCATC 
TGCAGAGCCA TCCAGCGATT CCTGCTCGCC TACAAGGAGG AGCGCCGGGG GCCCACACTC 
ATCGCTGTTC AGTCCAGCTG GGAGCTGAAG AGGCTGGCCA GTGAAATTCC TGTCTTGGAG 
GAATTCCCAC TGGTGCCTAT CTGTGTGGCT GACAAGATCA ACTATGGGGT CCTGGACTGG 
CAGCGCCATG GAGCCCGGCG CATGATCCGT CACTACCTCA ACCTGGACAC CTGCCTGTCG 
CAGGCCTTCG AGATGAGCAG GTACTTTCAC ATTCCCATTG GGAACCTACC AGAGGACATC 
TCCACATTCG GCTCCGACCT CTTCTTTGCC CGCCACCTCC AGCGCCACAA CCACCTGCTC 
TGGCTGTCCC CTACAGCCCG CCCTGACCTG GGTGGAAAGG AGGCTGATGA CAACTGTCTT 
GTCATGGAGT TCGATGACCA AGCCACTGTT GAGATCAACA GTTCAGGCTG TTACTCCACA 
GTGTGTGTGG AGCTGGACCT TCAGAACCTG GCCGTCAACA CCATTCTCCA GTCTCACCAT 
GTCAACGACA TGGAGGGGGC CGACAGCATG GGGATCAGCT TCGACGTGAT CCAGCAGGCC 
TCCCTGGAGG ACATGATCAC GGGTGGTCAG GCTGCCAGTG CCCCGGCCAG CTACGATGAG 
ACAGCCCTGT GCTCTAACAC CTTCAGGATC CTGAAGAGCA TGGTCGTGGG CTGGGTGAAG 
GAGATCACCC AGTACCACAA CATCTATGCA GACAACCAGG TGATGCACTT CTACCGCTGG 
CTTCGGTCGC CATCCTCTCT GCTTCATGAC CCTGCCCTGC ACCGCACACT CCACAACATG 
ATGAAGAAGC TCTTCCTGCA GCTCATCGCT GAGTTCAAGC GCCTGGGGTC ATCAGTCATC 
TACGCCAACT TCAACCGCAT CATCCTCTGT ACAAAGAAGC GCCGTGTGGA AGATGCCATC 
GCTTACGTGG AGTACATCAC CAGCAGCATC CATTCAAAGG AGACCTTCCA TTCTCTGACA 
ATTTCTTTCT CTCGATGCTG GGAATTTCTT CTCTGGATGG ATCCATCTAA CTATGGCGGA 
ATCAAAGGAA AAGTTTCATC TCGTATTCAC TGTGGACTGC AAGACTCCCA GAAAGCAGGG 
GGAGCAGAGG ATGAGCAGGA AAATGAGGAC GATGAGGAGG AAAGAGATGG GGAGGAGGAG 
GAAGAGGCGG AGGAATCCAA CGTGGAGGAT TTACTGGAAA ACAACTGGAA CATTTTGCAG 
TTTTTGCCAC AGGCAGCCTC CTGCCAGAAC TACTTCCTCA TGATTGTTTC AGCGTACATC 
GTGGCCGTGT ACCACTGCAT GAAGGACGGG CTGAGGCGCA GTGCTCCAGG GAGCACCCCC 
GTGAGGAGGA GGGGGGCCAG CCAGCTCTCC CAGGAGGCCG AGGGGGCGGT CGGAGCCCTT 
CCCGGAATGA TCACCTTCTC TCAGGATTAT GTCGCAAATG AGCTCACTCA GAGCTTCTTC 
ACCATCACTC AGAAGATTCA GAAGAAAGTC ACAGGCTCTC GGAACTCCAC TGAGCTCTCA 
GAGATGTTTC CTGTCCTCCC CGGTTCCCAC TTGCTGCTCA ATAACCCTGC CCTGGAGTTC 
ATCAAATACG TGTGCAAGGT GCTGTCCCTG GACACCAACA TCACAAACCA GGTGAATAAG 
CTGAACCGAG ACCTGCTTCG CCTGGTGGAT GTCGGCGAGT TCTCCGAGGA GGCCCAGTTC 
CGAGACCCCT GCCGCTCCTA CGTGCTTCCT GAGGTCATCT GCCGCAGCTG TAACTTCTGC 
CGCGACCTGG ACCTGTGTAA AGACTCTTCC TTCTCAGAGG ATGGGGCGGT CCTGCCTCAG 
TGGCTCTGCT CCAACTGTCA GGCGCCCTAC GACTCCTCTG CCATCGAGAT GACGCTGGTG 
GAAGTTCTAC AGAAGAAGCT GATGGCCTTC ACCCTGCAGG ACCTGCGGAA GGAGGCAGAC 
ACTCCCAAGT GTGAAGAGAA GGAAAGGAGG AAGAGGGAGT TGCACAGCTC GGCCACAGGA 
AGGTCTTGCC GAGGGGCATC TGAGCAGAGG CTGGGGGACG CGCCAAGCTG CCGTGTGGTG 
GTGCACGGCG TTCAGGCCTG CAGGATGGAG GGAGTGAGCA GGGCCTGGAG GAGGCCTCAG 
GACTGGGCAT CTGGGGTGGA ACATCCACAG CGAGGGTGGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_011534802.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533104.1 Homo sapiens polymerase (DNA) epsilon, catalytic subunit (POLE), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu59815
Clone ID Related Accession (Same CDS sequence)	XM_011534802.3 , XM_011534802.2
Accession Version	XM_011534802.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DNA polymerase epsilon catalytic subunit A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCAAG AAGGGGACCC CGTGCTTCCT GGGCTCCTGG AGACCAAGGC AGCCAACATG 
CCTGACTCTG AGCTATTCGA GCTCATCTCT GAGAACCGTT CCATGTCTCG GAAGCTGGAA 
GATTACGGGG AGCAGAAGTC TACGTCCATC AGCACAGCAA AGCGCCTGGC CGAGTTCCTG 
GGAGACCAGA TGGTCAAGGA TGCAGGGCTG AGTTGCCGCT ACATCATCTC CCGCAAGCCC 
GAGGGCTCCC CTGTCACGGA GAGGGCCATC CCACTTGCCA TTTTCCAAGC AGAGCCCACG 
GTGAGGAAGC ACTTTCTCCG GAAATGGCTC AAGAGCTCTT CCCTTCAAGA CTTTGATATT 
CGAGCAATTC TGGATTGGGA CTACTACATT GAGCGGCTGG GAAGCGCCAT CCAGAAGATC 
ATCACCATCC CTGCGGCCCT GCAGCAGGTA AAGAACCCAG TGCCACGTGT CAAACACCCC 
GACTGGCTGC ACAAAAAACT GCTGGAGAAG AATGATGTCT ACAAGCAGAA GAAGATCAGT 
GAGCTCTTCA CCCTGGAGGG CAGGAGACAG GTCACGATGG CCGAGGCCTC AGAAGACAGT 
CCGAGGCCAA GTGCTCCTGA CATGGAGGAC TTCGGCCTCG TAAAGCTGCC TCACCCAGCA 
GCCCCTGTCA CTGTGAAGAG GAAGCGAGTT CTTTGGGAGA GCCAGGAGGA GTCCCAGGAC 
CTCACGCCGA CTGTGCCCTG GCAGGAAATC TTGGGGCAGC CTCCCGCCCT GGGAACCAGC 
CAGGAGGAAT GGCTTGTCTG GCTCCGGTTC CACAAGAAGA AGTGGCAGCT GCAGGCCCGG 
CAGCGCCTCG CCCGCAGGAA GAGGCAGCGT CTGGAGTCGG CAGAGGGTGT GCTCAGGCCC 
GGGGCCATCC GGGATGGTCC TGCCACGGGG CTGGGGAGCT TCTTGCGAAG AACTGCCCGC 
AGCATCCTGG ACCTTCCGTG GCAGATTGTG CAGATCAGCG AGACCAGCCA GGCCGGCCTG 
TTCAGGCTGT GGGCGCTCGT TGGCAGTGAC TTGCACTGCA TCAGGCTGAG CATCCCCCGT 
GTGTTCTACG TGAACCAGCG AGTCGCTAAA GCGGAGGAGG GTGCTTCGTA TCGCAAGGTA 
AATCGGGTCC TTCCTCGCTC CAACATGGTC TACAATCTCT ATGAGTATTC AGTGCCAGAG 
GACATGTACC AGGAACACAT CAACGAGATC AACGCTGAGC TGTCAGCGCC AGACATCGAG 
GGCGTATATG AGACTCAGGT TCCGTTACTG TTCCGGGCCC TGGTGCACCT GGGCTGTGTG 
TGTGTGGTCA ATAAACAGCT GGTGAGGCAC CTTTCAGGCT GGGAAGCAGA GACCTTTGCT 
CTTGAGCACC TGGAGATGCG CTCTCTGGCC CAGTTCAGCT ACCTGGAACC AGGGAGTATC 
CGCCATATCT ACCTGTACCA CCACGCACAG GCCCACAAAG CGCTCTTCGG GATCTTCATC 
CCCTCACAGC GCAGGGCATC CGTCTTTGTG CTGGACACTG TGCGCAGCAA CCAGATGCCC 
AGCCTTGGCG CCCTGTACTC AGCAGAGCAC GGCCTCCTCC TGGAGAAGGT GGGCCCTGAG 
CTCCTGCCAC CCCCCAAACA CACCTTCGAA GTTCGGGCAG AAACTGACCT GAAGACCATC 
TGCAGAGCCA TCCAGCGATT CCTGCTCGCC TACAAGGAGG AGCGCCGGGG GCCCACACTC 
ATCGCTGTTC AGTCCAGCTG GGAGCTGAAG AGGCTGGCCA GTGAAATTCC TGTCTTGGAG 
GAATTCCCAC TGGTGCCTAT CTGTGTGGCT GACAAGATCA ACTATGGGGT CCTGGACTGG 
CAGCGCCATG GAGCCCGGCG CATGATCCGT CACTACCTCA ACCTGGACAC CTGCCTGTCG 
CAGGCCTTCG AGATGAGCAG GTACTTTCAC ATTCCCATTG GGAACCTACC AGAGGACATC 
TCCACATTCG GCTCCGACCT CTTCTTTGCC CGCCACCTCC AGCGCCACAA CCACCTGCTC 
TGGCTGTCCC CTACAGCCCG CCCTGACCTG GGTGGAAAGG AGGCTGATGA CAACTGTCTT 
GTCATGGAGT TCGATGACCA AGCCACTGTT GAGATCAACA GTTCAGGCTG TTACTCCACA 
GTGTGTGTGG AGCTGGACCT TCAGAACCTG GCCGTCAACA CCATTCTCCA GTCTCACCAT 
GTCAACGACA TGGAGGGGGC CGACAGCATG GGGATCAGCT TCGACGTGAT CCAGCAGGCC 
TCCCTGGAGG ACATGATCAC GGGTGGTCAG GCTGCCAGTG CCCCGGCCAG CTACGATGAG 
ACAGCCCTGT GCTCTAACAC CTTCAGGATC CTGAAGAGCA TGGTCGTGGG CTGGGTGAAG 
GAGATCACCC AGTACCACAA CATCTATGCA GACAACCAGG TGATGCACTT CTACCGCTGG 
CTTCGGTCGC CATCCTCTCT GCTTCATGAC CCTGCCCTGC ACCGCACACT CCACAACATG 
ATGAAGAAGC TCTTCCTGCA GCTCATCGCT GAGTTCAAGC GCCTGGGGTC ATCAGTCATC 
TACGCCAACT TCAACCGCAT CATCCTCTGT ACAAAGAAGC GCCGTGTGGA AGATGCCATC 
GCTTACGTGG AGTACATCAC CAGCAGCATC CATTCAAAGG AGACCTTCCA TTCTCTGACA 
ATTTCTTTCT CTCGATGCTG GGAATTTCTT CTCTGGATGG ATCCATCTAA CTATGGCGGA 
ATCAAAGGAA AAGTTTCATC TCGTATTCAC TGTGGACTGC AAGACTCCCA GAAAGCAGGG 
GGAGCAGAGG ATGAGCAGGA AAATGAGGAC GATGAGGAGG AAAGAGATGG GGAGGAGGAG 
GAAGAGGCGG AGGAATCCAA CGTGGAGGAT TTACTGGAAA ACAACTGGAA CATTTTGCAG 
TTTTTGCCAC AGGCAGCCTC CTGCCAGAAC TACTTCCTCA TGATTGTTTC AGCGTACATC 
GTGGCCGTGT ACCACTGCAT GAAGGACGGG CTGAGGCGCA GTGCTCCAGG GAGCACCCCC 
GTGAGGAGGA GGGGGGCCAG CCAGCTCTCC CAGGAGGCCG AGGGGGCGGT CGGAGCCCTT 
CCCGGAATGA TCACCTTCTC TCAGGATTAT GTCGCAAATG AGCTCACTCA GAGCTTCTTC 
ACCATCACTC AGAAGATTCA GAAGAAAGTC ACAGGCTCTC GGAACTCCAC TGAGCTCTCA 
GAGATGTTTC CTGTCCTCCC CGGTTCCCAC TTGCTGCTCA ATAACCCTGC CCTGGAGTTC 
ATCAAATACG TGTGCAAGGT GCTGTCCCTG GACACCAACA TCACAAACCA GGTGAATAAG 
CTGAACCGAG ACCTGCTTCG CCTGGTGGAT GTCGGCGAGT TCTCCGAGGA GGCCCAGTTC 
CGAGACCCCT GCCGCTCCTA CGTGCTTCCT GAGGTCATCT GCCGCAGCTG TAACTTCTGC 
CGCGACCTGG ACCTGTGTAA AGACTCTTCC TTCTCAGAGG ATGGGGCGGT CCTGCCTCAG 
TGGCTCTGCT CCAACTGTCA GGCGCCCTAC GACTCCTCTG CCATCGAGAT GACGCTGGTG 
GAAGTTCTAC AGAAGAAGCT GATGGCCTTC ACCCTGCAGG ACCTGCGGAA GGAGGCAGAC 
ACTCCCAAGT GTGAAGAGAA GGAAAGGAGG AAGAGGGAGT TGCACAGCTC GGCCACAGGA 
AGGTCTTGCC GAGGGGCATC TGAGCAGAGG CTGGGGGACG CGCCAAGCTG CCGTGTGGTG 
GTGCACGGCG TTCAGGCCTG CAGGATGGAG GGAGTGAGCA GGGCCTGGAG GAGGCCTCAG 
GACTGGGCAT CTGGGGTGGA ACATCCACAG CGAGGGTGGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533104.1
CDS	39..3923
Translation	MKQEGDPVLPGLLETKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRAILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFTLEGRRQVTMAEASEDSPRPSAPDMEDFGLVKLPHPAAPVTVKRKRVLWESQEESQDLTPTVPWQEILGQPPALGTSQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEGVLRPGAIRDGPATGLGSFLRRTARSILDLPWQIVQISETSQAGLFRLWALVGSDLHCIRLSIPRVFYVNQRVAKAEEGASYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINAELSAPDIEGVYETQVPLLFRALVHLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHAQAHKALFGIFIPSQRRASVFVLDTVRSNQMPSLGALYSAEHGLLLEKVGPELLPPPKHTFEVRAETDLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELKRLASEIPVLEEFPLVPICVADKINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTARPDLGGKEADDNCLVMEFDDQATVEINSSGCYSTVCVELDLQNLAVNTILQSHHVNDMEGADSMGISFDVIQQASLEDMITGGQAASAPASYDETALCSNTFRILKSMVVGWVKEITQYHNIYADNQVMHFYRWLRSPSSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVIYANFNRIILCTKKRRVEDAIAYVEYITSSIHSKETFHSLTISFSRCWEFLLWMDPSNYGGIKGKVSSRIHCGLQDSQKAGGAEDEQENEDDEEERDGEEEEEAEESNVEDLLENNWNILQFLPQAASCQNYFLMIVSAYIVAVYHCMKDGLRRSAPGSTPVRRRGASQLSQEAEGAVGALPGMITFSQDYVANELTQSFFTITQKIQKKVTGSRNSTELSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCKDSSFSEDGAVLPQWLCSNCQAPYDSSAIEMTLVEVLQKKLMAFTLQDLRKEADTPKCEEKERRKRELHSSATGRSCRGASEQRLGDAPSCRVVVHGVQACRMEGVSRAWRRPQDWASGVEHPQRGWV

Target ORF information:

RefSeq Version	XM_011534802.2
Organism	Homo sapiens(human)
Definition	Homo sapiens polymerase (DNA) epsilon, catalytic subunit (POLE), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534802.2

ORF Insert Sequence:

ATGAAGCAAG AAGGGGACCC CGTGCTTCCT GGGCTCCTGG AGACCAAGGC AGCCAACATG 
CCTGACTCTG AGCTATTCGA GCTCATCTCT GAGAACCGTT CCATGTCTCG GAAGCTGGAA 
GATTACGGGG AGCAGAAGTC TACGTCCATC AGCACAGCAA AGCGCCTGGC CGAGTTCCTG 
GGAGACCAGA TGGTCAAGGA TGCAGGGCTG AGTTGCCGCT ACATCATCTC CCGCAAGCCC 
GAGGGCTCCC CTGTCACGGA GAGGGCCATC CCACTTGCCA TTTTCCAAGC AGAGCCCACG 
GTGAGGAAGC ACTTTCTCCG GAAATGGCTC AAGAGCTCTT CCCTTCAAGA CTTTGATATT 
CGAGCAATTC TGGATTGGGA CTACTACATT GAGCGGCTGG GAAGCGCCAT CCAGAAGATC 
ATCACCATCC CTGCGGCCCT GCAGCAGGTA AAGAACCCAG TGCCACGTGT CAAACACCCC 
GACTGGCTGC ACAAAAAACT GCTGGAGAAG AATGATGTCT ACAAGCAGAA GAAGATCAGT 
GAGCTCTTCA CCCTGGAGGG CAGGAGACAG GTCACGATGG CCGAGGCCTC AGAAGACAGT 
CCGAGGCCAA GTGCTCCTGA CATGGAGGAC TTCGGCCTCG TAAAGCTGCC TCACCCAGCA 
GCCCCTGTCA CTGTGAAGAG GAAGCGAGTT CTTTGGGAGA GCCAGGAGGA GTCCCAGGAC 
CTCACGCCGA CTGTGCCCTG GCAGGAAATC TTGGGGCAGC CTCCCGCCCT GGGAACCAGC 
CAGGAGGAAT GGCTTGTCTG GCTCCGGTTC CACAAGAAGA AGTGGCAGCT GCAGGCCCGG 
CAGCGCCTCG CCCGCAGGAA GAGGCAGCGT CTGGAGTCGG CAGAGGGTGT GCTCAGGCCC 
GGGGCCATCC GGGATGGTCC TGCCACGGGG CTGGGGAGCT TCTTGCGAAG AACTGCCCGC 
AGCATCCTGG ACCTTCCGTG GCAGATTGTG CAGATCAGCG AGACCAGCCA GGCCGGCCTG 
TTCAGGCTGT GGGCGCTCGT TGGCAGTGAC TTGCACTGCA TCAGGCTGAG CATCCCCCGT 
GTGTTCTACG TGAACCAGCG AGTCGCTAAA GCGGAGGAGG GTGCTTCGTA TCGCAAGGTA 
AATCGGGTCC TTCCTCGCTC CAACATGGTC TACAATCTCT ATGAGTATTC AGTGCCAGAG 
GACATGTACC AGGAACACAT CAACGAGATC AACGCTGAGC TGTCAGCGCC AGACATCGAG 
GGCGTATATG AGACTCAGGT TCCGTTACTG TTCCGGGCCC TGGTGCACCT GGGCTGTGTG 
TGTGTGGTCA ATAAACAGCT GGTGAGGCAC CTTTCAGGCT GGGAAGCAGA GACCTTTGCT 
CTTGAGCACC TGGAGATGCG CTCTCTGGCC CAGTTCAGCT ACCTGGAACC AGGGAGTATC 
CGCCATATCT ACCTGTACCA CCACGCACAG GCCCACAAAG CGCTCTTCGG GATCTTCATC 
CCCTCACAGC GCAGGGCATC CGTCTTTGTG CTGGACACTG TGCGCAGCAA CCAGATGCCC 
AGCCTTGGCG CCCTGTACTC AGCAGAGCAC GGCCTCCTCC TGGAGAAGGT GGGCCCTGAG 
CTCCTGCCAC CCCCCAAACA CACCTTCGAA GTTCGGGCAG AAACTGACCT GAAGACCATC 
TGCAGAGCCA TCCAGCGATT CCTGCTCGCC TACAAGGAGG AGCGCCGGGG GCCCACACTC 
ATCGCTGTTC AGTCCAGCTG GGAGCTGAAG AGGCTGGCCA GTGAAATTCC TGTCTTGGAG 
GAATTCCCAC TGGTGCCTAT CTGTGTGGCT GACAAGATCA ACTATGGGGT CCTGGACTGG 
CAGCGCCATG GAGCCCGGCG CATGATCCGT CACTACCTCA ACCTGGACAC CTGCCTGTCG 
CAGGCCTTCG AGATGAGCAG GTACTTTCAC ATTCCCATTG GGAACCTACC AGAGGACATC 
TCCACATTCG GCTCCGACCT CTTCTTTGCC CGCCACCTCC AGCGCCACAA CCACCTGCTC 
TGGCTGTCCC CTACAGCCCG CCCTGACCTG GGTGGAAAGG AGGCTGATGA CAACTGTCTT 
GTCATGGAGT TCGATGACCA AGCCACTGTT GAGATCAACA GTTCAGGCTG TTACTCCACA 
GTGTGTGTGG AGCTGGACCT TCAGAACCTG GCCGTCAACA CCATTCTCCA GTCTCACCAT 
GTCAACGACA TGGAGGGGGC CGACAGCATG GGGATCAGCT TCGACGTGAT CCAGCAGGCC 
TCCCTGGAGG ACATGATCAC GGGTGGTCAG GCTGCCAGTG CCCCGGCCAG CTACGATGAG 
ACAGCCCTGT GCTCTAACAC CTTCAGGATC CTGAAGAGCA TGGTCGTGGG CTGGGTGAAG 
GAGATCACCC AGTACCACAA CATCTATGCA GACAACCAGG TGATGCACTT CTACCGCTGG 
CTTCGGTCGC CATCCTCTCT GCTTCATGAC CCTGCCCTGC ACCGCACACT CCACAACATG 
ATGAAGAAGC TCTTCCTGCA GCTCATCGCT GAGTTCAAGC GCCTGGGGTC ATCAGTCATC 
TACGCCAACT TCAACCGCAT CATCCTCTGT ACAAAGAAGC GCCGTGTGGA AGATGCCATC 
GCTTACGTGG AGTACATCAC CAGCAGCATC CATTCAAAGG AGACCTTCCA TTCTCTGACA 
ATTTCTTTCT CTCGATGCTG GGAATTTCTT CTCTGGATGG ATCCATCTAA CTATGGCGGA 
ATCAAAGGAA AAGTTTCATC TCGTATTCAC TGTGGACTGC AAGACTCCCA GAAAGCAGGG 
GGAGCAGAGG ATGAGCAGGA AAATGAGGAC GATGAGGAGG AAAGAGATGG GGAGGAGGAG 
GAAGAGGCGG AGGAATCCAA CGTGGAGGAT TTACTGGAAA ACAACTGGAA CATTTTGCAG 
TTTTTGCCAC AGGCAGCCTC CTGCCAGAAC TACTTCCTCA TGATTGTTTC AGCGTACATC 
GTGGCCGTGT ACCACTGCAT GAAGGACGGG CTGAGGCGCA GTGCTCCAGG GAGCACCCCC 
GTGAGGAGGA GGGGGGCCAG CCAGCTCTCC CAGGAGGCCG AGGGGGCGGT CGGAGCCCTT 
CCCGGAATGA TCACCTTCTC TCAGGATTAT GTCGCAAATG AGCTCACTCA GAGCTTCTTC 
ACCATCACTC AGAAGATTCA GAAGAAAGTC ACAGGCTCTC GGAACTCCAC TGAGCTCTCA 
GAGATGTTTC CTGTCCTCCC CGGTTCCCAC TTGCTGCTCA ATAACCCTGC CCTGGAGTTC 
ATCAAATACG TGTGCAAGGT GCTGTCCCTG GACACCAACA TCACAAACCA GGTGAATAAG 
CTGAACCGAG ACCTGCTTCG CCTGGTGGAT GTCGGCGAGT TCTCCGAGGA GGCCCAGTTC 
CGAGACCCCT GCCGCTCCTA CGTGCTTCCT GAGGTCATCT GCCGCAGCTG TAACTTCTGC 
CGCGACCTGG ACCTGTGTAA AGACTCTTCC TTCTCAGAGG ATGGGGCGGT CCTGCCTCAG 
TGGCTCTGCT CCAACTGTCA GGCGCCCTAC GACTCCTCTG CCATCGAGAT GACGCTGGTG 
GAAGTTCTAC AGAAGAAGCT GATGGCCTTC ACCCTGCAGG ACCTGCGGAA GGAGGCAGAC 
ACTCCCAAGT GTGAAGAGAA GGAAAGGAGG AAGAGGGAGT TGCACAGCTC GGCCACAGGA 
AGGTCTTGCC GAGGGGCATC TGAGCAGAGG CTGGGGGACG CGCCAAGCTG CCGTGTGGTG 
GTGCACGGCG TTCAGGCCTG CAGGATGGAG GGAGTGAGCA GGGCCTGGAG GAGGCCTCAG 
GACTGGGCAT CTGGGGTGGA ACATCCACAG CGAGGGTGGG TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_011534797.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533099.1 Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.

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CloneID	OHu59810
Clone ID Related Accession (Same CDS sequence)	XM_011534797.3 , XM_011534797.2
Accession Version	XM_011534797.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA polymerase epsilon catalytic subunit A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011534797.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGG AGATTGTTTC AGAAGATATT GAAGATTTTG AGTTCACCCC CAAGCCAGAA 
TATGAAGGCC CCTTTTGTGT CTTCAATGAA CCCGATGAGG CTCATCTGAT CCAAAGGTGG 
TTTGAACACG TCCAGGAGAC CAAACCCACC ATCATGGTCA CCTACAACGG GGACTTTTTT 
GACTGGCCAT TTGTGGAGGC CCGGGCAGCA GTCCACGGTC TGAGCATGCA GCAGGAGATA 
GGCTTCCAGA AGGACAGCCA GGGGGAGTAC AAGGCGCCCC AGTGCATCCA CATGGACTGC 
CTCAGGTGGG TGAAGAGGGA CAGTTACCTT CCTGTGGGCA GTCATAATCT CAAGGCGGCC 
GCCAAGGCCA AGCTAGGCTA TGATCCCGTG GAGCTAGACC CGGAGGACAT GTGCCGGATG 
GCCACGGAGC AGCCCCAGAC TCTGGCCACG TATTCTGTGT CAGATGCTGT CGCCACTTAC 
TACCTGTACA TGAAGTACGT CCACCCATTC ATCTTTGCTC TGTGCACCAT TATTCCCATG 
GAGCCCGACG AGGTGCTGCG GAAGGGCTCT GGCACTCTGT GTGAGGCCTT GCTGATGGTG 
CAGGCCTTCC ACGCCAACAT CATCTTCCCC AACAAGCAAG AGCAGGAGTT CAATAAGCTG 
ACGGACGACG GACACGTGCT GGACTCTGAG ACCTACGTCG GGGGCCACGT GGAGGCCCTC 
GAGTCTGGGG TTTTCCGCAG CGATATCCCT TGCCGGTTTA GGATGAATCC TGCCGCCTTT 
GACTTCCTGC TGCAGCGGGT TGAGAAGACC TTGCGCCACG CCCTTGAGGA AGAGGAGAAA 
GTGCCTGTGG AGCAAGTCAC CAACTTTGAA GAGGTGTGTG ATGAGATTAA GAGCAAGCTT 
GCCTCCCTGA AGGACGTTCC CAGCCGCATC GAGTGTCCAC TCATCTACCA CCTGGACGTG 
GGGGCCATGT ACCCCAACAT CATCCTGACC AACCGCCTGC AGCCCTCTGC CATGGTGGAC 
GAAGCCACCT GTGCTGCCTG TGACTTCAAT AAGCCTGGAG CAAACTGCCA GCGGAAGATG 
GCCTGGCAGT GGAGGGGCGA GTTCATGCCA GCCAGTCGCA GCGAATACCA TCGGATCCAG 
CACCAGCTGG AGTCAGAGAA GTTCCCCCCC TTGTTCCCAG AGGGGCCAGC TCGGGCCTTT 
CATGAACTGT CCCGCGAGGA ACAGGCGAAA TACGAGAAGA GAAGGCTGGC GGATTACTGC 
CGGAAAGCCT ACAAGAAGAT CCACATCACC AAGGTGGAAG AGCGTCTCAC CACCATCTGC 
CAGCGGGAAA ACTCCTTCTA CGTGGACACC GTGCGTGCCT TCCGGGACAG GCGTTACGAG 
TTCAAAGGGC TCCACAAGGT GTGGAAAAAG AAGCTCTCGG CGGCCGTGGA GGTGGGCGAC 
GCGGCTGAGG TGAAGCGCTG CAAGAACATG GAGGTGCTGT ATGACTCGCT GCAGCTGGCC 
CACAAGTGCA TCCTGAACTC CTTCTATGGC TATGTCATGC GCAAGGGGGC TCGCTGGTAC 
TCCATGGAGA TGGCTGGCAT CGTCTGCTTC ACAGGGGCCA ACATCATCAC CCAGGCACGG 
GAGCTGATCG AGCAGATTGG GAGGCCCTTA GAGCTGGACA CAGATGGTAT ATGGTGCGTC 
CTGCCCAACA GCTTCCCAGA AAATTTTGTC TTCAAGACGA CCAATGTGAA GAAGCCCAAA 
GTGACCATCT CCTACCCAGG CGCCATGTTG AACATCATGG TCAAGGAAGG CTTCACCAAT 
GACCAGTACC AGGAGCTGGC TGAGCCGTCC TCACTCACCT ACGTCACCCG CTCAGAGAAC 
AGCATCTTTT TTGAGGTTGA TGGGCCCTAC CTTGCCATGA TTCTTCCAGC CTCCAAGGAA 
GAAGGCAAGA AATTGAAGAA GAGGTATGCT GTGTTCAATG AAGACGGTTC TCTGGCTGAG 
CTCAAGGGCT TTGAGGTCAA ACGCCGCGGG GAACTGCAGC TGATTAAGAT CTTCCAATCC 
TCGGTGTTTG AGGCCTTCCT CAAGGGCAGC ACGCTGGAAG AGGTGTATGG CTCTGTAGCC 
AAGGTGGCTG ACTACTGGCT GGACGTGCTG TACAGCAAGG CAGCCAACAT GCCTGACTCT 
GAGCTATTCG AGCTCATCTC TGAGAACCGT TCCATGTCTC GGAAGCTGGA AGATTACGGG 
GAGCAGAAGT CTACGTCCAT CAGCACAGCA AAGCGCCTGG CCGAGTTCCT GGGAGACCAG 
ATGGTCAAGG ATGCAGGGCT GAGTTGCCGC TACATCATCT CCCGCAAGCC CGAGGGCTCC 
CCTGTCACGG AGAGGGCCAT CCCACTTGCC ATTTTCCAAG CAGAGCCCAC GGTGAGGAAG 
CACTTTCTCC GGAAATGGCT CAAGAGCTCT TCCCTTCAAG ACTTTGATAT TCGAGCAATT 
CTGGATTGGG ACTACTACAT TGAGCGGCTG GGAAGCGCCA TCCAGAAGAT CATCACCATC 
CCTGCGGCCC TGCAGCAGGT AAAGAACCCA GTGCCACGTG TCAAACACCC CGACTGGCTG 
CACAAAAAAC TGCTGGAGAA GAATGATGTC TACAAGCAGA AGAAGATCAG TGAGCTCTTC 
ACCCTGGAGG GCAGGAGACA GGTCACGATG GCCGAGGCCT CAGAAGACAG TCCGAGGCCA 
AGTGCTCCTG ACATGGAGGA CTTCGGCCTC GTAAAGCTGC CTCACCCAGC AGCCCCTGTC 
ACTGTGAAGA GGAAGCGAGT TCTTTGGGAG AGCCAGGAGG AGTCCCAGGA CCTCACGCCG 
ACTGTGCCCT GGCAGGAAAT CTTGGGGCAG CCTCCCGCCC TGGGAACCAG CCAGGAGGAA 
TGGCTTGTCT GGCTCCGGTT CCACAAGAAG AAGTGGCAGC TGCAGGCCCG GCAGCGCCTC 
GCCCGCAGGA AGAGGCAGCG TCTGGAGTCG GCAGAGGGTG TGCTCAGGCC CGGGGCCATC 
CGGGATGGTC CTGCCACGGG GCTGGGGAGC TTCTTGCGAA GAACTGCCCG CAGCATCCTG 
GACCTTCCGT GGCAGATTGT GCAGATCAGC GAGACCAGCC AGGCCGGCCT GTTCAGGCTG 
TGGGCGCTCG TTGGCAGTGA CTTGCACTGC ATCAGGCTGA GCATCCCCCG TGTGTTCTAC 
GTGAACCAGC GAGTCGCTAA AGCGGAGGAG GGTGCTTCGT ATCGCAAGGT AAATCGGGTC 
CTTCCTCGCT CCAACATGGT CTACAATCTC TATGAGTATT CAGTGCCAGA GGACATGTAC 
CAGGAACACA TCAACGAGAT CAACGCTGAG CTGTCAGCGC CAGACATCGA GGGCGTATAT 
GAGACTCAGG TTCCGTTACT GTTCCGGGCC CTGGTGCACC TGGGCTGTGT GTGTGTGGTC 
AATAAACAGC TGGTGAGGCA CCTTTCAGGC TGGGAAGCAG AGACCTTTGC TCTTGAGCAC 
CTGGAGATGC GCTCTCTGGC CCAGTTCAGC TACCTGGAAC CAGGGAGTAT CCGCCATATC 
TACCTGTACC ACCACGCACA GGCCCACAAA GCGCTCTTCG GGATCTTCAT CCCCTCACAG 
CGCAGGGCAT CCGTCTTTGT GCTGGACACT GTGCGCAGCA ACCAGATGCC CAGCCTTGGC 
GCCCTGTACT CAGCAGAGCA CGGCCTCCTC CTGGAGAAGG TGGGCCCTGA GCTCCTGCCA 
CCCCCCAAAC ACACCTTCGA AGTTCGGGCA GAAACTGACC TGAAGACCAT CTGCAGAGCC 
ATCCAGCGAT TCCTGCTCGC CTACAAGGAG GAGCGCCGGG GGCCCACACT CATCGCTGTT 
CAGTCCAGCT GGGAGCTGAA GAGGCTGGCC AGTGAAATTC CTGTCTTGGA GGAATTCCCA 
CTGGTGCCTA TCTGTGTGGC TGACAAGATC AACTATGGGG TCCTGGACTG GCAGCGCCAT 
GGAGCCCGGC GCATGATCCG TCACTACCTC AACCTGGACA CCTGCCTGTC GCAGGCCTTC 
GAGATGAGCA GGTACTTTCA CATTCCCATT GGGAACCTAC CAGAGGACAT CTCCACATTC 
GGCTCCGACC TCTTCTTTGC CCGCCACCTC CAGCGCCACA ACCACCTGCT CTGGCTGTCC 
CCTACAGCCC GCCCTGACCT GGGTGGAAAG GAGGCTGATG ACAACTGTCT TGTCATGGAG 
TTCGATGACC AAGCCACTGT TGAGATCAAC AGTTCAGGCT GTTACTCCAC AGTGTGTGTG 
GAGCTGGACC TTCAGAACCT GGCCGTCAAC ACCATTCTCC AGTCTCACCA TGTCAACGAC 
ATGGAGGGGG CCGACAGCAT GGGGATCAGC TTCGACGTGA TCCAGCAGGC CTCCCTGGAG 
GACATGATCA CGGGTGGTCA GGCTGCCAGT GCCCCGGCCA GCTACGATGA GACAGCCCTG 
TGCTCTAACA CCTTCAGGAT CCTGAAGAGC ATGGTCGTGG GCTGGGTGAA GGAGATCACC 
CAGTACCACA ACATCTATGC AGACAACCAG GTGATGCACT TCTACCGCTG GCTTCGGTCG 
CCATCCTCTC TGCTTCATGA CCCTGCCCTG CACCGCACAC TCCACAACAT GATGAAGAAG 
CTCTTCCTGC AGCTCATCGC TGAGTTCAAG CGCCTGGGGT CATCAGTCAT CTACGCCAAC 
TTCAACCGCA TCATCCTCTG TACAAAGAAG CGCCGTGTGG AAGATGCCAT CGCTTACGTG 
GAGTACATCA CCAGCAGCAT CCATTCAAAG GAGACCTTCC ATTCTCTGAC AATTTCTTTC 
TCTCGATGCT GGGAATTTCT TCTCTGGATG GATCCATCTA ACTATGGCGG AATCAAAGGA 
AAAGTTTCAT CTCGTATTCA CTGTGGACTG CAAGACTCCC AGAAAGCAGG GGGAGCAGAG 
GATGAGCAGG AAAATGAGGA CGATGAGGAG GAAAGAGATG GGGAGGAGGA GGAAGAGGCG 
GAGGAATCCA ACGTGGAGGA TTTACTGGAA AACAACTGGA ACATTTTGCA GTTTTTGCCA 
CAGGCAGCCT CCTGCCAGAA CTACTTCCTC ATGATTGTTT CAGCGTACAT CGTGGCCGTG 
TACCACTGCA TGAAGGACGG GCTGAGGCGC AGTGCTCCAG GGAGCACCCC CGTGAGGAGG 
AGGGGGGCCA GCCAGCTCTC CCAGGAGGCC GAGGGGGCGG TCGGAGCCCT TCCCGGAATG 
ATCACCTTCT CTCAGGATTA TGTCGCAAAT GAGCTCACTC AGAGCTTCTT CACCATCACT 
CAGAAGATTC AGAAGAAAGT CACAGGCTCT CGGAACTCCA CTGAGCTCTC AGAGATGTTT 
CCTGTCCTCC CCGGTTCCCA CTTGCTGCTC AATAACCCTG CCCTGGAGTT CATCAAATAC 
GTGTGCAAGG TGCTGTCCCT GGACACCAAC ATCACAAACC AGGTGAATAA GCTGAACCGA 
GACCTGCTTC GCCTGGTGGA TGTCGGCGAG TTCTCCGAGG AGGCCCAGTT CCGAGACCCC 
TGCCGCTCCT ACGTGCTTCC TGAGGTCATC TGCCGCAGCT GTAACTTCTG CCGCGACCTG 
GACCTGTGTA AAGACTCTTC CTTCTCAGAG GATGGGGCGG TCCTGCCTCA GTGGCTCTGC 
TCCAACTGTC AGGCGCCCTA CGACTCCTCT GCCATCGAGA TGACGCTGGT GGAAGTTCTA 
CAGAAGAAGC TGATGGCCTT CACCCTGCAG GACCTGCGGA AGGAGGCAGA CACTCCCAAG 
TGTGAAGAGA AGGAAAGGAG GAAGAGGGAG TTGCACAGCT CGGCCACAGG AAGGTCTTGC 
CGAGGGGCAT CTGAGCAGAG GCTGGGGGAC GCGCCAAGCT GCCGTGTGGT GGTGCACGGC 
GTTCAGGCCT GCAGGATGGA GGGAGTGAGC AGGGCCTGGA GGAGGCCTCA GGACTGGGCA 
TCTGGGGTGG AACATCCACA GCGAGGGTGG GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533099.1
CDS	95..6070
Translation	MAREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEAHLIQRWFEHVQETKPTIMVTYNGDFFDWPFVEARAAVHGLSMQQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDSETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTLRHALEEEEKVPVEQVTNFEEVCDEIKSKLASLKDVPSRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHITKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVFKTTNVKKPKVTISYPGAMLNIMVKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRAILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFTLEGRRQVTMAEASEDSPRPSAPDMEDFGLVKLPHPAAPVTVKRKRVLWESQEESQDLTPTVPWQEILGQPPALGTSQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEGVLRPGAIRDGPATGLGSFLRRTARSILDLPWQIVQISETSQAGLFRLWALVGSDLHCIRLSIPRVFYVNQRVAKAEEGASYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINAELSAPDIEGVYETQVPLLFRALVHLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHAQAHKALFGIFIPSQRRASVFVLDTVRSNQMPSLGALYSAEHGLLLEKVGPELLPPPKHTFEVRAETDLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELKRLASEIPVLEEFPLVPICVADKINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTARPDLGGKEADDNCLVMEFDDQATVEINSSGCYSTVCVELDLQNLAVNTILQSHHVNDMEGADSMGISFDVIQQASLEDMITGGQAASAPASYDETALCSNTFRILKSMVVGWVKEITQYHNIYADNQVMHFYRWLRSPSSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVIYANFNRIILCTKKRRVEDAIAYVEYITSSIHSKETFHSLTISFSRCWEFLLWMDPSNYGGIKGKVSSRIHCGLQDSQKAGGAEDEQENEDDEEERDGEEEEEAEESNVEDLLENNWNILQFLPQAASCQNYFLMIVSAYIVAVYHCMKDGLRRSAPGSTPVRRRGASQLSQEAEGAVGALPGMITFSQDYVANELTQSFFTITQKIQKKVTGSRNSTELSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCKDSSFSEDGAVLPQWLCSNCQAPYDSSAIEMTLVEVLQKKLMAFTLQDLRKEADTPKCEEKERRKRELHSSATGRSCRGASEQRLGDAPSCRVVVHGVQACRMEGVSRAWRRPQDWASGVEHPQRGWV

Target ORF information:

RefSeq Version	XM_011534797.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534797.3

ORF Insert Sequence:

ATGGCCAGGG AGATTGTTTC AGAAGATATT GAAGATTTTG AGTTCACCCC CAAGCCAGAA 
TATGAAGGCC CCTTTTGTGT CTTCAATGAA CCCGATGAGG CTCATCTGAT CCAAAGGTGG 
TTTGAACACG TCCAGGAGAC CAAACCCACC ATCATGGTCA CCTACAACGG GGACTTTTTT 
GACTGGCCAT TTGTGGAGGC CCGGGCAGCA GTCCACGGTC TGAGCATGCA GCAGGAGATA 
GGCTTCCAGA AGGACAGCCA GGGGGAGTAC AAGGCGCCCC AGTGCATCCA CATGGACTGC 
CTCAGGTGGG TGAAGAGGGA CAGTTACCTT CCTGTGGGCA GTCATAATCT CAAGGCGGCC 
GCCAAGGCCA AGCTAGGCTA TGATCCCGTG GAGCTAGACC CGGAGGACAT GTGCCGGATG 
GCCACGGAGC AGCCCCAGAC TCTGGCCACG TATTCTGTGT CAGATGCTGT CGCCACTTAC 
TACCTGTACA TGAAGTACGT CCACCCATTC ATCTTTGCTC TGTGCACCAT TATTCCCATG 
GAGCCCGACG AGGTGCTGCG GAAGGGCTCT GGCACTCTGT GTGAGGCCTT GCTGATGGTG 
CAGGCCTTCC ACGCCAACAT CATCTTCCCC AACAAGCAAG AGCAGGAGTT CAATAAGCTG 
ACGGACGACG GACACGTGCT GGACTCTGAG ACCTACGTCG GGGGCCACGT GGAGGCCCTC 
GAGTCTGGGG TTTTCCGCAG CGATATCCCT TGCCGGTTTA GGATGAATCC TGCCGCCTTT 
GACTTCCTGC TGCAGCGGGT TGAGAAGACC TTGCGCCACG CCCTTGAGGA AGAGGAGAAA 
GTGCCTGTGG AGCAAGTCAC CAACTTTGAA GAGGTGTGTG ATGAGATTAA GAGCAAGCTT 
GCCTCCCTGA AGGACGTTCC CAGCCGCATC GAGTGTCCAC TCATCTACCA CCTGGACGTG 
GGGGCCATGT ACCCCAACAT CATCCTGACC AACCGCCTGC AGCCCTCTGC CATGGTGGAC 
GAAGCCACCT GTGCTGCCTG TGACTTCAAT AAGCCTGGAG CAAACTGCCA GCGGAAGATG 
GCCTGGCAGT GGAGGGGCGA GTTCATGCCA GCCAGTCGCA GCGAATACCA TCGGATCCAG 
CACCAGCTGG AGTCAGAGAA GTTCCCCCCC TTGTTCCCAG AGGGGCCAGC TCGGGCCTTT 
CATGAACTGT CCCGCGAGGA ACAGGCGAAA TACGAGAAGA GAAGGCTGGC GGATTACTGC 
CGGAAAGCCT ACAAGAAGAT CCACATCACC AAGGTGGAAG AGCGTCTCAC CACCATCTGC 
CAGCGGGAAA ACTCCTTCTA CGTGGACACC GTGCGTGCCT TCCGGGACAG GCGTTACGAG 
TTCAAAGGGC TCCACAAGGT GTGGAAAAAG AAGCTCTCGG CGGCCGTGGA GGTGGGCGAC 
GCGGCTGAGG TGAAGCGCTG CAAGAACATG GAGGTGCTGT ATGACTCGCT GCAGCTGGCC 
CACAAGTGCA TCCTGAACTC CTTCTATGGC TATGTCATGC GCAAGGGGGC TCGCTGGTAC 
TCCATGGAGA TGGCTGGCAT CGTCTGCTTC ACAGGGGCCA ACATCATCAC CCAGGCACGG 
GAGCTGATCG AGCAGATTGG GAGGCCCTTA GAGCTGGACA CAGATGGTAT ATGGTGCGTC 
CTGCCCAACA GCTTCCCAGA AAATTTTGTC TTCAAGACGA CCAATGTGAA GAAGCCCAAA 
GTGACCATCT CCTACCCAGG CGCCATGTTG AACATCATGG TCAAGGAAGG CTTCACCAAT 
GACCAGTACC AGGAGCTGGC TGAGCCGTCC TCACTCACCT ACGTCACCCG CTCAGAGAAC 
AGCATCTTTT TTGAGGTTGA TGGGCCCTAC CTTGCCATGA TTCTTCCAGC CTCCAAGGAA 
GAAGGCAAGA AATTGAAGAA GAGGTATGCT GTGTTCAATG AAGACGGTTC TCTGGCTGAG 
CTCAAGGGCT TTGAGGTCAA ACGCCGCGGG GAACTGCAGC TGATTAAGAT CTTCCAATCC 
TCGGTGTTTG AGGCCTTCCT CAAGGGCAGC ACGCTGGAAG AGGTGTATGG CTCTGTAGCC 
AAGGTGGCTG ACTACTGGCT GGACGTGCTG TACAGCAAGG CAGCCAACAT GCCTGACTCT 
GAGCTATTCG AGCTCATCTC TGAGAACCGT TCCATGTCTC GGAAGCTGGA AGATTACGGG 
GAGCAGAAGT CTACGTCCAT CAGCACAGCA AAGCGCCTGG CCGAGTTCCT GGGAGACCAG 
ATGGTCAAGG ATGCAGGGCT GAGTTGCCGC TACATCATCT CCCGCAAGCC CGAGGGCTCC 
CCTGTCACGG AGAGGGCCAT CCCACTTGCC ATTTTCCAAG CAGAGCCCAC GGTGAGGAAG 
CACTTTCTCC GGAAATGGCT CAAGAGCTCT TCCCTTCAAG ACTTTGATAT TCGAGCAATT 
CTGGATTGGG ACTACTACAT TGAGCGGCTG GGAAGCGCCA TCCAGAAGAT CATCACCATC 
CCTGCGGCCC TGCAGCAGGT AAAGAACCCA GTGCCACGTG TCAAACACCC CGACTGGCTG 
CACAAAAAAC TGCTGGAGAA GAATGATGTC TACAAGCAGA AGAAGATCAG TGAGCTCTTC 
ACCCTGGAGG GCAGGAGACA GGTCACGATG GCCGAGGCCT CAGAAGACAG TCCGAGGCCA 
AGTGCTCCTG ACATGGAGGA CTTCGGCCTC GTAAAGCTGC CTCACCCAGC AGCCCCTGTC 
ACTGTGAAGA GGAAGCGAGT TCTTTGGGAG AGCCAGGAGG AGTCCCAGGA CCTCACGCCG 
ACTGTGCCCT GGCAGGAAAT CTTGGGGCAG CCTCCCGCCC TGGGAACCAG CCAGGAGGAA 
TGGCTTGTCT GGCTCCGGTT CCACAAGAAG AAGTGGCAGC TGCAGGCCCG GCAGCGCCTC 
GCCCGCAGGA AGAGGCAGCG TCTGGAGTCG GCAGAGGGTG TGCTCAGGCC CGGGGCCATC 
CGGGATGGTC CTGCCACGGG GCTGGGGAGC TTCTTGCGAA GAACTGCCCG CAGCATCCTG 
GACCTTCCGT GGCAGATTGT GCAGATCAGC GAGACCAGCC AGGCCGGCCT GTTCAGGCTG 
TGGGCGCTCG TTGGCAGTGA CTTGCACTGC ATCAGGCTGA GCATCCCCCG TGTGTTCTAC 
GTGAACCAGC GAGTCGCTAA AGCGGAGGAG GGTGCTTCGT ATCGCAAGGT AAATCGGGTC 
CTTCCTCGCT CCAACATGGT CTACAATCTC TATGAGTATT CAGTGCCAGA GGACATGTAC 
CAGGAACACA TCAACGAGAT CAACGCTGAG CTGTCAGCGC CAGACATCGA GGGCGTATAT 
GAGACTCAGG TTCCGTTACT GTTCCGGGCC CTGGTGCACC TGGGCTGTGT GTGTGTGGTC 
AATAAACAGC TGGTGAGGCA CCTTTCAGGC TGGGAAGCAG AGACCTTTGC TCTTGAGCAC 
CTGGAGATGC GCTCTCTGGC CCAGTTCAGC TACCTGGAAC CAGGGAGTAT CCGCCATATC 
TACCTGTACC ACCACGCACA GGCCCACAAA GCGCTCTTCG GGATCTTCAT CCCCTCACAG 
CGCAGGGCAT CCGTCTTTGT GCTGGACACT GTGCGCAGCA ACCAGATGCC CAGCCTTGGC 
GCCCTGTACT CAGCAGAGCA CGGCCTCCTC CTGGAGAAGG TGGGCCCTGA GCTCCTGCCA 
CCCCCCAAAC ACACCTTCGA AGTTCGGGCA GAAACTGACC TGAAGACCAT CTGCAGAGCC 
ATCCAGCGAT TCCTGCTCGC CTACAAGGAG GAGCGCCGGG GGCCCACACT CATCGCTGTT 
CAGTCCAGCT GGGAGCTGAA GAGGCTGGCC AGTGAAATTC CTGTCTTGGA GGAATTCCCA 
CTGGTGCCTA TCTGTGTGGC TGACAAGATC AACTATGGGG TCCTGGACTG GCAGCGCCAT 
GGAGCCCGGC GCATGATCCG TCACTACCTC AACCTGGACA CCTGCCTGTC GCAGGCCTTC 
GAGATGAGCA GGTACTTTCA CATTCCCATT GGGAACCTAC CAGAGGACAT CTCCACATTC 
GGCTCCGACC TCTTCTTTGC CCGCCACCTC CAGCGCCACA ACCACCTGCT CTGGCTGTCC 
CCTACAGCCC GCCCTGACCT GGGTGGAAAG GAGGCTGATG ACAACTGTCT TGTCATGGAG 
TTCGATGACC AAGCCACTGT TGAGATCAAC AGTTCAGGCT GTTACTCCAC AGTGTGTGTG 
GAGCTGGACC TTCAGAACCT GGCCGTCAAC ACCATTCTCC AGTCTCACCA TGTCAACGAC 
ATGGAGGGGG CCGACAGCAT GGGGATCAGC TTCGACGTGA TCCAGCAGGC CTCCCTGGAG 
GACATGATCA CGGGTGGTCA GGCTGCCAGT GCCCCGGCCA GCTACGATGA GACAGCCCTG 
TGCTCTAACA CCTTCAGGAT CCTGAAGAGC ATGGTCGTGG GCTGGGTGAA GGAGATCACC 
CAGTACCACA ACATCTATGC AGACAACCAG GTGATGCACT TCTACCGCTG GCTTCGGTCG 
CCATCCTCTC TGCTTCATGA CCCTGCCCTG CACCGCACAC TCCACAACAT GATGAAGAAG 
CTCTTCCTGC AGCTCATCGC TGAGTTCAAG CGCCTGGGGT CATCAGTCAT CTACGCCAAC 
TTCAACCGCA TCATCCTCTG TACAAAGAAG CGCCGTGTGG AAGATGCCAT CGCTTACGTG 
GAGTACATCA CCAGCAGCAT CCATTCAAAG GAGACCTTCC ATTCTCTGAC AATTTCTTTC 
TCTCGATGCT GGGAATTTCT TCTCTGGATG GATCCATCTA ACTATGGCGG AATCAAAGGA 
AAAGTTTCAT CTCGTATTCA CTGTGGACTG CAAGACTCCC AGAAAGCAGG GGGAGCAGAG 
GATGAGCAGG AAAATGAGGA CGATGAGGAG GAAAGAGATG GGGAGGAGGA GGAAGAGGCG 
GAGGAATCCA ACGTGGAGGA TTTACTGGAA AACAACTGGA ACATTTTGCA GTTTTTGCCA 
CAGGCAGCCT CCTGCCAGAA CTACTTCCTC ATGATTGTTT CAGCGTACAT CGTGGCCGTG 
TACCACTGCA TGAAGGACGG GCTGAGGCGC AGTGCTCCAG GGAGCACCCC CGTGAGGAGG 
AGGGGGGCCA GCCAGCTCTC CCAGGAGGCC GAGGGGGCGG TCGGAGCCCT TCCCGGAATG 
ATCACCTTCT CTCAGGATTA TGTCGCAAAT GAGCTCACTC AGAGCTTCTT CACCATCACT 
CAGAAGATTC AGAAGAAAGT CACAGGCTCT CGGAACTCCA CTGAGCTCTC AGAGATGTTT 
CCTGTCCTCC CCGGTTCCCA CTTGCTGCTC AATAACCCTG CCCTGGAGTT CATCAAATAC 
GTGTGCAAGG TGCTGTCCCT GGACACCAAC ATCACAAACC AGGTGAATAA GCTGAACCGA 
GACCTGCTTC GCCTGGTGGA TGTCGGCGAG TTCTCCGAGG AGGCCCAGTT CCGAGACCCC 
TGCCGCTCCT ACGTGCTTCC TGAGGTCATC TGCCGCAGCT GTAACTTCTG CCGCGACCTG 
GACCTGTGTA AAGACTCTTC CTTCTCAGAG GATGGGGCGG TCCTGCCTCA GTGGCTCTGC 
TCCAACTGTC AGGCGCCCTA CGACTCCTCT GCCATCGAGA TGACGCTGGT GGAAGTTCTA 
CAGAAGAAGC TGATGGCCTT CACCCTGCAG GACCTGCGGA AGGAGGCAGA CACTCCCAAG 
TGTGAAGAGA AGGAAAGGAG GAAGAGGGAG TTGCACAGCT CGGCCACAGG AAGGTCTTGC 
CGAGGGGCAT CTGAGCAGAG GCTGGGGGAC GCGCCAAGCT GCCGTGTGGT GGTGCACGGC 
GTTCAGGCCT GCAGGATGGA GGGAGTGAGC AGGGCCTGGA GGAGGCCTCA GGACTGGGCA 
TCTGGGGTGG AACATCCACA GCGAGGGTGG GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_011534797.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533099.1 Homo sapiens polymerase (DNA) epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.

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CloneID	OHu59810
Clone ID Related Accession (Same CDS sequence)	XM_011534797.3 , XM_011534797.2
Accession Version	XM_011534797.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DNA polymerase epsilon catalytic subunit A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534797.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGG AGATTGTTTC AGAAGATATT GAAGATTTTG AGTTCACCCC CAAGCCAGAA 
TATGAAGGCC CCTTTTGTGT CTTCAATGAA CCCGATGAGG CTCATCTGAT CCAAAGGTGG 
TTTGAACACG TCCAGGAGAC CAAACCCACC ATCATGGTCA CCTACAACGG GGACTTTTTT 
GACTGGCCAT TTGTGGAGGC CCGGGCAGCA GTCCACGGTC TGAGCATGCA GCAGGAGATA 
GGCTTCCAGA AGGACAGCCA GGGGGAGTAC AAGGCGCCCC AGTGCATCCA CATGGACTGC 
CTCAGGTGGG TGAAGAGGGA CAGTTACCTT CCTGTGGGCA GTCATAATCT CAAGGCGGCC 
GCCAAGGCCA AGCTAGGCTA TGATCCCGTG GAGCTAGACC CGGAGGACAT GTGCCGGATG 
GCCACGGAGC AGCCCCAGAC TCTGGCCACG TATTCTGTGT CAGATGCTGT CGCCACTTAC 
TACCTGTACA TGAAGTACGT CCACCCATTC ATCTTTGCTC TGTGCACCAT TATTCCCATG 
GAGCCCGACG AGGTGCTGCG GAAGGGCTCT GGCACTCTGT GTGAGGCCTT GCTGATGGTG 
CAGGCCTTCC ACGCCAACAT CATCTTCCCC AACAAGCAAG AGCAGGAGTT CAATAAGCTG 
ACGGACGACG GACACGTGCT GGACTCTGAG ACCTACGTCG GGGGCCACGT GGAGGCCCTC 
GAGTCTGGGG TTTTCCGCAG CGATATCCCT TGCCGGTTTA GGATGAATCC TGCCGCCTTT 
GACTTCCTGC TGCAGCGGGT TGAGAAGACC TTGCGCCACG CCCTTGAGGA AGAGGAGAAA 
GTGCCTGTGG AGCAAGTCAC CAACTTTGAA GAGGTGTGTG ATGAGATTAA GAGCAAGCTT 
GCCTCCCTGA AGGACGTTCC CAGCCGCATC GAGTGTCCAC TCATCTACCA CCTGGACGTG 
GGGGCCATGT ACCCCAACAT CATCCTGACC AACCGCCTGC AGCCCTCTGC CATGGTGGAC 
GAAGCCACCT GTGCTGCCTG TGACTTCAAT AAGCCTGGAG CAAACTGCCA GCGGAAGATG 
GCCTGGCAGT GGAGGGGCGA GTTCATGCCA GCCAGTCGCA GCGAATACCA TCGGATCCAG 
CACCAGCTGG AGTCAGAGAA GTTCCCCCCC TTGTTCCCAG AGGGGCCAGC TCGGGCCTTT 
CATGAACTGT CCCGCGAGGA ACAGGCGAAA TACGAGAAGA GAAGGCTGGC GGATTACTGC 
CGGAAAGCCT ACAAGAAGAT CCACATCACC AAGGTGGAAG AGCGTCTCAC CACCATCTGC 
CAGCGGGAAA ACTCCTTCTA CGTGGACACC GTGCGTGCCT TCCGGGACAG GCGTTACGAG 
TTCAAAGGGC TCCACAAGGT GTGGAAAAAG AAGCTCTCGG CGGCCGTGGA GGTGGGCGAC 
GCGGCTGAGG TGAAGCGCTG CAAGAACATG GAGGTGCTGT ATGACTCGCT GCAGCTGGCC 
CACAAGTGCA TCCTGAACTC CTTCTATGGC TATGTCATGC GCAAGGGGGC TCGCTGGTAC 
TCCATGGAGA TGGCTGGCAT CGTCTGCTTC ACAGGGGCCA ACATCATCAC CCAGGCACGG 
GAGCTGATCG AGCAGATTGG GAGGCCCTTA GAGCTGGACA CAGATGGTAT ATGGTGCGTC 
CTGCCCAACA GCTTCCCAGA AAATTTTGTC TTCAAGACGA CCAATGTGAA GAAGCCCAAA 
GTGACCATCT CCTACCCAGG CGCCATGTTG AACATCATGG TCAAGGAAGG CTTCACCAAT 
GACCAGTACC AGGAGCTGGC TGAGCCGTCC TCACTCACCT ACGTCACCCG CTCAGAGAAC 
AGCATCTTTT TTGAGGTTGA TGGGCCCTAC CTTGCCATGA TTCTTCCAGC CTCCAAGGAA 
GAAGGCAAGA AATTGAAGAA GAGGTATGCT GTGTTCAATG AAGACGGTTC TCTGGCTGAG 
CTCAAGGGCT TTGAGGTCAA ACGCCGCGGG GAACTGCAGC TGATTAAGAT CTTCCAATCC 
TCGGTGTTTG AGGCCTTCCT CAAGGGCAGC ACGCTGGAAG AGGTGTATGG CTCTGTAGCC 
AAGGTGGCTG ACTACTGGCT GGACGTGCTG TACAGCAAGG CAGCCAACAT GCCTGACTCT 
GAGCTATTCG AGCTCATCTC TGAGAACCGT TCCATGTCTC GGAAGCTGGA AGATTACGGG 
GAGCAGAAGT CTACGTCCAT CAGCACAGCA AAGCGCCTGG CCGAGTTCCT GGGAGACCAG 
ATGGTCAAGG ATGCAGGGCT GAGTTGCCGC TACATCATCT CCCGCAAGCC CGAGGGCTCC 
CCTGTCACGG AGAGGGCCAT CCCACTTGCC ATTTTCCAAG CAGAGCCCAC GGTGAGGAAG 
CACTTTCTCC GGAAATGGCT CAAGAGCTCT TCCCTTCAAG ACTTTGATAT TCGAGCAATT 
CTGGATTGGG ACTACTACAT TGAGCGGCTG GGAAGCGCCA TCCAGAAGAT CATCACCATC 
CCTGCGGCCC TGCAGCAGGT AAAGAACCCA GTGCCACGTG TCAAACACCC CGACTGGCTG 
CACAAAAAAC TGCTGGAGAA GAATGATGTC TACAAGCAGA AGAAGATCAG TGAGCTCTTC 
ACCCTGGAGG GCAGGAGACA GGTCACGATG GCCGAGGCCT CAGAAGACAG TCCGAGGCCA 
AGTGCTCCTG ACATGGAGGA CTTCGGCCTC GTAAAGCTGC CTCACCCAGC AGCCCCTGTC 
ACTGTGAAGA GGAAGCGAGT TCTTTGGGAG AGCCAGGAGG AGTCCCAGGA CCTCACGCCG 
ACTGTGCCCT GGCAGGAAAT CTTGGGGCAG CCTCCCGCCC TGGGAACCAG CCAGGAGGAA 
TGGCTTGTCT GGCTCCGGTT CCACAAGAAG AAGTGGCAGC TGCAGGCCCG GCAGCGCCTC 
GCCCGCAGGA AGAGGCAGCG TCTGGAGTCG GCAGAGGGTG TGCTCAGGCC CGGGGCCATC 
CGGGATGGTC CTGCCACGGG GCTGGGGAGC TTCTTGCGAA GAACTGCCCG CAGCATCCTG 
GACCTTCCGT GGCAGATTGT GCAGATCAGC GAGACCAGCC AGGCCGGCCT GTTCAGGCTG 
TGGGCGCTCG TTGGCAGTGA CTTGCACTGC ATCAGGCTGA GCATCCCCCG TGTGTTCTAC 
GTGAACCAGC GAGTCGCTAA AGCGGAGGAG GGTGCTTCGT ATCGCAAGGT AAATCGGGTC 
CTTCCTCGCT CCAACATGGT CTACAATCTC TATGAGTATT CAGTGCCAGA GGACATGTAC 
CAGGAACACA TCAACGAGAT CAACGCTGAG CTGTCAGCGC CAGACATCGA GGGCGTATAT 
GAGACTCAGG TTCCGTTACT GTTCCGGGCC CTGGTGCACC TGGGCTGTGT GTGTGTGGTC 
AATAAACAGC TGGTGAGGCA CCTTTCAGGC TGGGAAGCAG AGACCTTTGC TCTTGAGCAC 
CTGGAGATGC GCTCTCTGGC CCAGTTCAGC TACCTGGAAC CAGGGAGTAT CCGCCATATC 
TACCTGTACC ACCACGCACA GGCCCACAAA GCGCTCTTCG GGATCTTCAT CCCCTCACAG 
CGCAGGGCAT CCGTCTTTGT GCTGGACACT GTGCGCAGCA ACCAGATGCC CAGCCTTGGC 
GCCCTGTACT CAGCAGAGCA CGGCCTCCTC CTGGAGAAGG TGGGCCCTGA GCTCCTGCCA 
CCCCCCAAAC ACACCTTCGA AGTTCGGGCA GAAACTGACC TGAAGACCAT CTGCAGAGCC 
ATCCAGCGAT TCCTGCTCGC CTACAAGGAG GAGCGCCGGG GGCCCACACT CATCGCTGTT 
CAGTCCAGCT GGGAGCTGAA GAGGCTGGCC AGTGAAATTC CTGTCTTGGA GGAATTCCCA 
CTGGTGCCTA TCTGTGTGGC TGACAAGATC AACTATGGGG TCCTGGACTG GCAGCGCCAT 
GGAGCCCGGC GCATGATCCG TCACTACCTC AACCTGGACA CCTGCCTGTC GCAGGCCTTC 
GAGATGAGCA GGTACTTTCA CATTCCCATT GGGAACCTAC CAGAGGACAT CTCCACATTC 
GGCTCCGACC TCTTCTTTGC CCGCCACCTC CAGCGCCACA ACCACCTGCT CTGGCTGTCC 
CCTACAGCCC GCCCTGACCT GGGTGGAAAG GAGGCTGATG ACAACTGTCT TGTCATGGAG 
TTCGATGACC AAGCCACTGT TGAGATCAAC AGTTCAGGCT GTTACTCCAC AGTGTGTGTG 
GAGCTGGACC TTCAGAACCT GGCCGTCAAC ACCATTCTCC AGTCTCACCA TGTCAACGAC 
ATGGAGGGGG CCGACAGCAT GGGGATCAGC TTCGACGTGA TCCAGCAGGC CTCCCTGGAG 
GACATGATCA CGGGTGGTCA GGCTGCCAGT GCCCCGGCCA GCTACGATGA GACAGCCCTG 
TGCTCTAACA CCTTCAGGAT CCTGAAGAGC ATGGTCGTGG GCTGGGTGAA GGAGATCACC 
CAGTACCACA ACATCTATGC AGACAACCAG GTGATGCACT TCTACCGCTG GCTTCGGTCG 
CCATCCTCTC TGCTTCATGA CCCTGCCCTG CACCGCACAC TCCACAACAT GATGAAGAAG 
CTCTTCCTGC AGCTCATCGC TGAGTTCAAG CGCCTGGGGT CATCAGTCAT CTACGCCAAC 
TTCAACCGCA TCATCCTCTG TACAAAGAAG CGCCGTGTGG AAGATGCCAT CGCTTACGTG 
GAGTACATCA CCAGCAGCAT CCATTCAAAG GAGACCTTCC ATTCTCTGAC AATTTCTTTC 
TCTCGATGCT GGGAATTTCT TCTCTGGATG GATCCATCTA ACTATGGCGG AATCAAAGGA 
AAAGTTTCAT CTCGTATTCA CTGTGGACTG CAAGACTCCC AGAAAGCAGG GGGAGCAGAG 
GATGAGCAGG AAAATGAGGA CGATGAGGAG GAAAGAGATG GGGAGGAGGA GGAAGAGGCG 
GAGGAATCCA ACGTGGAGGA TTTACTGGAA AACAACTGGA ACATTTTGCA GTTTTTGCCA 
CAGGCAGCCT CCTGCCAGAA CTACTTCCTC ATGATTGTTT CAGCGTACAT CGTGGCCGTG 
TACCACTGCA TGAAGGACGG GCTGAGGCGC AGTGCTCCAG GGAGCACCCC CGTGAGGAGG 
AGGGGGGCCA GCCAGCTCTC CCAGGAGGCC GAGGGGGCGG TCGGAGCCCT TCCCGGAATG 
ATCACCTTCT CTCAGGATTA TGTCGCAAAT GAGCTCACTC AGAGCTTCTT CACCATCACT 
CAGAAGATTC AGAAGAAAGT CACAGGCTCT CGGAACTCCA CTGAGCTCTC AGAGATGTTT 
CCTGTCCTCC CCGGTTCCCA CTTGCTGCTC AATAACCCTG CCCTGGAGTT CATCAAATAC 
GTGTGCAAGG TGCTGTCCCT GGACACCAAC ATCACAAACC AGGTGAATAA GCTGAACCGA 
GACCTGCTTC GCCTGGTGGA TGTCGGCGAG TTCTCCGAGG AGGCCCAGTT CCGAGACCCC 
TGCCGCTCCT ACGTGCTTCC TGAGGTCATC TGCCGCAGCT GTAACTTCTG CCGCGACCTG 
GACCTGTGTA AAGACTCTTC CTTCTCAGAG GATGGGGCGG TCCTGCCTCA GTGGCTCTGC 
TCCAACTGTC AGGCGCCCTA CGACTCCTCT GCCATCGAGA TGACGCTGGT GGAAGTTCTA 
CAGAAGAAGC TGATGGCCTT CACCCTGCAG GACCTGCGGA AGGAGGCAGA CACTCCCAAG 
TGTGAAGAGA AGGAAAGGAG GAAGAGGGAG TTGCACAGCT CGGCCACAGG AAGGTCTTGC 
CGAGGGGCAT CTGAGCAGAG GCTGGGGGAC GCGCCAAGCT GCCGTGTGGT GGTGCACGGC 
GTTCAGGCCT GCAGGATGGA GGGAGTGAGC AGGGCCTGGA GGAGGCCTCA GGACTGGGCA 
TCTGGGGTGG AACATCCACA GCGAGGGTGG GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533099.1
CDS	95..6070
Translation	MAREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEAHLIQRWFEHVQETKPTIMVTYNGDFFDWPFVEARAAVHGLSMQQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDSETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTLRHALEEEEKVPVEQVTNFEEVCDEIKSKLASLKDVPSRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHITKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVFKTTNVKKPKVTISYPGAMLNIMVKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRAILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFTLEGRRQVTMAEASEDSPRPSAPDMEDFGLVKLPHPAAPVTVKRKRVLWESQEESQDLTPTVPWQEILGQPPALGTSQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEGVLRPGAIRDGPATGLGSFLRRTARSILDLPWQIVQISETSQAGLFRLWALVGSDLHCIRLSIPRVFYVNQRVAKAEEGASYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINAELSAPDIEGVYETQVPLLFRALVHLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHAQAHKALFGIFIPSQRRASVFVLDTVRSNQMPSLGALYSAEHGLLLEKVGPELLPPPKHTFEVRAETDLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELKRLASEIPVLEEFPLVPICVADKINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTARPDLGGKEADDNCLVMEFDDQATVEINSSGCYSTVCVELDLQNLAVNTILQSHHVNDMEGADSMGISFDVIQQASLEDMITGGQAASAPASYDETALCSNTFRILKSMVVGWVKEITQYHNIYADNQVMHFYRWLRSPSSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVIYANFNRIILCTKKRRVEDAIAYVEYITSSIHSKETFHSLTISFSRCWEFLLWMDPSNYGGIKGKVSSRIHCGLQDSQKAGGAEDEQENEDDEEERDGEEEEEAEESNVEDLLENNWNILQFLPQAASCQNYFLMIVSAYIVAVYHCMKDGLRRSAPGSTPVRRRGASQLSQEAEGAVGALPGMITFSQDYVANELTQSFFTITQKIQKKVTGSRNSTELSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCKDSSFSEDGAVLPQWLCSNCQAPYDSSAIEMTLVEVLQKKLMAFTLQDLRKEADTPKCEEKERRKRELHSSATGRSCRGASEQRLGDAPSCRVVVHGVQACRMEGVSRAWRRPQDWASGVEHPQRGWV

Target ORF information:

RefSeq Version	XM_011534797.2
Organism	Homo sapiens(human)
Definition	Homo sapiens polymerase (DNA) epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534797.2

ORF Insert Sequence:

ATGGCCAGGG AGATTGTTTC AGAAGATATT GAAGATTTTG AGTTCACCCC CAAGCCAGAA 
TATGAAGGCC CCTTTTGTGT CTTCAATGAA CCCGATGAGG CTCATCTGAT CCAAAGGTGG 
TTTGAACACG TCCAGGAGAC CAAACCCACC ATCATGGTCA CCTACAACGG GGACTTTTTT 
GACTGGCCAT TTGTGGAGGC CCGGGCAGCA GTCCACGGTC TGAGCATGCA GCAGGAGATA 
GGCTTCCAGA AGGACAGCCA GGGGGAGTAC AAGGCGCCCC AGTGCATCCA CATGGACTGC 
CTCAGGTGGG TGAAGAGGGA CAGTTACCTT CCTGTGGGCA GTCATAATCT CAAGGCGGCC 
GCCAAGGCCA AGCTAGGCTA TGATCCCGTG GAGCTAGACC CGGAGGACAT GTGCCGGATG 
GCCACGGAGC AGCCCCAGAC TCTGGCCACG TATTCTGTGT CAGATGCTGT CGCCACTTAC 
TACCTGTACA TGAAGTACGT CCACCCATTC ATCTTTGCTC TGTGCACCAT TATTCCCATG 
GAGCCCGACG AGGTGCTGCG GAAGGGCTCT GGCACTCTGT GTGAGGCCTT GCTGATGGTG 
CAGGCCTTCC ACGCCAACAT CATCTTCCCC AACAAGCAAG AGCAGGAGTT CAATAAGCTG 
ACGGACGACG GACACGTGCT GGACTCTGAG ACCTACGTCG GGGGCCACGT GGAGGCCCTC 
GAGTCTGGGG TTTTCCGCAG CGATATCCCT TGCCGGTTTA GGATGAATCC TGCCGCCTTT 
GACTTCCTGC TGCAGCGGGT TGAGAAGACC TTGCGCCACG CCCTTGAGGA AGAGGAGAAA 
GTGCCTGTGG AGCAAGTCAC CAACTTTGAA GAGGTGTGTG ATGAGATTAA GAGCAAGCTT 
GCCTCCCTGA AGGACGTTCC CAGCCGCATC GAGTGTCCAC TCATCTACCA CCTGGACGTG 
GGGGCCATGT ACCCCAACAT CATCCTGACC AACCGCCTGC AGCCCTCTGC CATGGTGGAC 
GAAGCCACCT GTGCTGCCTG TGACTTCAAT AAGCCTGGAG CAAACTGCCA GCGGAAGATG 
GCCTGGCAGT GGAGGGGCGA GTTCATGCCA GCCAGTCGCA GCGAATACCA TCGGATCCAG 
CACCAGCTGG AGTCAGAGAA GTTCCCCCCC TTGTTCCCAG AGGGGCCAGC TCGGGCCTTT 
CATGAACTGT CCCGCGAGGA ACAGGCGAAA TACGAGAAGA GAAGGCTGGC GGATTACTGC 
CGGAAAGCCT ACAAGAAGAT CCACATCACC AAGGTGGAAG AGCGTCTCAC CACCATCTGC 
CAGCGGGAAA ACTCCTTCTA CGTGGACACC GTGCGTGCCT TCCGGGACAG GCGTTACGAG 
TTCAAAGGGC TCCACAAGGT GTGGAAAAAG AAGCTCTCGG CGGCCGTGGA GGTGGGCGAC 
GCGGCTGAGG TGAAGCGCTG CAAGAACATG GAGGTGCTGT ATGACTCGCT GCAGCTGGCC 
CACAAGTGCA TCCTGAACTC CTTCTATGGC TATGTCATGC GCAAGGGGGC TCGCTGGTAC 
TCCATGGAGA TGGCTGGCAT CGTCTGCTTC ACAGGGGCCA ACATCATCAC CCAGGCACGG 
GAGCTGATCG AGCAGATTGG GAGGCCCTTA GAGCTGGACA CAGATGGTAT ATGGTGCGTC 
CTGCCCAACA GCTTCCCAGA AAATTTTGTC TTCAAGACGA CCAATGTGAA GAAGCCCAAA 
GTGACCATCT CCTACCCAGG CGCCATGTTG AACATCATGG TCAAGGAAGG CTTCACCAAT 
GACCAGTACC AGGAGCTGGC TGAGCCGTCC TCACTCACCT ACGTCACCCG CTCAGAGAAC 
AGCATCTTTT TTGAGGTTGA TGGGCCCTAC CTTGCCATGA TTCTTCCAGC CTCCAAGGAA 
GAAGGCAAGA AATTGAAGAA GAGGTATGCT GTGTTCAATG AAGACGGTTC TCTGGCTGAG 
CTCAAGGGCT TTGAGGTCAA ACGCCGCGGG GAACTGCAGC TGATTAAGAT CTTCCAATCC 
TCGGTGTTTG AGGCCTTCCT CAAGGGCAGC ACGCTGGAAG AGGTGTATGG CTCTGTAGCC 
AAGGTGGCTG ACTACTGGCT GGACGTGCTG TACAGCAAGG CAGCCAACAT GCCTGACTCT 
GAGCTATTCG AGCTCATCTC TGAGAACCGT TCCATGTCTC GGAAGCTGGA AGATTACGGG 
GAGCAGAAGT CTACGTCCAT CAGCACAGCA AAGCGCCTGG CCGAGTTCCT GGGAGACCAG 
ATGGTCAAGG ATGCAGGGCT GAGTTGCCGC TACATCATCT CCCGCAAGCC CGAGGGCTCC 
CCTGTCACGG AGAGGGCCAT CCCACTTGCC ATTTTCCAAG CAGAGCCCAC GGTGAGGAAG 
CACTTTCTCC GGAAATGGCT CAAGAGCTCT TCCCTTCAAG ACTTTGATAT TCGAGCAATT 
CTGGATTGGG ACTACTACAT TGAGCGGCTG GGAAGCGCCA TCCAGAAGAT CATCACCATC 
CCTGCGGCCC TGCAGCAGGT AAAGAACCCA GTGCCACGTG TCAAACACCC CGACTGGCTG 
CACAAAAAAC TGCTGGAGAA GAATGATGTC TACAAGCAGA AGAAGATCAG TGAGCTCTTC 
ACCCTGGAGG GCAGGAGACA GGTCACGATG GCCGAGGCCT CAGAAGACAG TCCGAGGCCA 
AGTGCTCCTG ACATGGAGGA CTTCGGCCTC GTAAAGCTGC CTCACCCAGC AGCCCCTGTC 
ACTGTGAAGA GGAAGCGAGT TCTTTGGGAG AGCCAGGAGG AGTCCCAGGA CCTCACGCCG 
ACTGTGCCCT GGCAGGAAAT CTTGGGGCAG CCTCCCGCCC TGGGAACCAG CCAGGAGGAA 
TGGCTTGTCT GGCTCCGGTT CCACAAGAAG AAGTGGCAGC TGCAGGCCCG GCAGCGCCTC 
GCCCGCAGGA AGAGGCAGCG TCTGGAGTCG GCAGAGGGTG TGCTCAGGCC CGGGGCCATC 
CGGGATGGTC CTGCCACGGG GCTGGGGAGC TTCTTGCGAA GAACTGCCCG CAGCATCCTG 
GACCTTCCGT GGCAGATTGT GCAGATCAGC GAGACCAGCC AGGCCGGCCT GTTCAGGCTG 
TGGGCGCTCG TTGGCAGTGA CTTGCACTGC ATCAGGCTGA GCATCCCCCG TGTGTTCTAC 
GTGAACCAGC GAGTCGCTAA AGCGGAGGAG GGTGCTTCGT ATCGCAAGGT AAATCGGGTC 
CTTCCTCGCT CCAACATGGT CTACAATCTC TATGAGTATT CAGTGCCAGA GGACATGTAC 
CAGGAACACA TCAACGAGAT CAACGCTGAG CTGTCAGCGC CAGACATCGA GGGCGTATAT 
GAGACTCAGG TTCCGTTACT GTTCCGGGCC CTGGTGCACC TGGGCTGTGT GTGTGTGGTC 
AATAAACAGC TGGTGAGGCA CCTTTCAGGC TGGGAAGCAG AGACCTTTGC TCTTGAGCAC 
CTGGAGATGC GCTCTCTGGC CCAGTTCAGC TACCTGGAAC CAGGGAGTAT CCGCCATATC 
TACCTGTACC ACCACGCACA GGCCCACAAA GCGCTCTTCG GGATCTTCAT CCCCTCACAG 
CGCAGGGCAT CCGTCTTTGT GCTGGACACT GTGCGCAGCA ACCAGATGCC CAGCCTTGGC 
GCCCTGTACT CAGCAGAGCA CGGCCTCCTC CTGGAGAAGG TGGGCCCTGA GCTCCTGCCA 
CCCCCCAAAC ACACCTTCGA AGTTCGGGCA GAAACTGACC TGAAGACCAT CTGCAGAGCC 
ATCCAGCGAT TCCTGCTCGC CTACAAGGAG GAGCGCCGGG GGCCCACACT CATCGCTGTT 
CAGTCCAGCT GGGAGCTGAA GAGGCTGGCC AGTGAAATTC CTGTCTTGGA GGAATTCCCA 
CTGGTGCCTA TCTGTGTGGC TGACAAGATC AACTATGGGG TCCTGGACTG GCAGCGCCAT 
GGAGCCCGGC GCATGATCCG TCACTACCTC AACCTGGACA CCTGCCTGTC GCAGGCCTTC 
GAGATGAGCA GGTACTTTCA CATTCCCATT GGGAACCTAC CAGAGGACAT CTCCACATTC 
GGCTCCGACC TCTTCTTTGC CCGCCACCTC CAGCGCCACA ACCACCTGCT CTGGCTGTCC 
CCTACAGCCC GCCCTGACCT GGGTGGAAAG GAGGCTGATG ACAACTGTCT TGTCATGGAG 
TTCGATGACC AAGCCACTGT TGAGATCAAC AGTTCAGGCT GTTACTCCAC AGTGTGTGTG 
GAGCTGGACC TTCAGAACCT GGCCGTCAAC ACCATTCTCC AGTCTCACCA TGTCAACGAC 
ATGGAGGGGG CCGACAGCAT GGGGATCAGC TTCGACGTGA TCCAGCAGGC CTCCCTGGAG 
GACATGATCA CGGGTGGTCA GGCTGCCAGT GCCCCGGCCA GCTACGATGA GACAGCCCTG 
TGCTCTAACA CCTTCAGGAT CCTGAAGAGC ATGGTCGTGG GCTGGGTGAA GGAGATCACC 
CAGTACCACA ACATCTATGC AGACAACCAG GTGATGCACT TCTACCGCTG GCTTCGGTCG 
CCATCCTCTC TGCTTCATGA CCCTGCCCTG CACCGCACAC TCCACAACAT GATGAAGAAG 
CTCTTCCTGC AGCTCATCGC TGAGTTCAAG CGCCTGGGGT CATCAGTCAT CTACGCCAAC 
TTCAACCGCA TCATCCTCTG TACAAAGAAG CGCCGTGTGG AAGATGCCAT CGCTTACGTG 
GAGTACATCA CCAGCAGCAT CCATTCAAAG GAGACCTTCC ATTCTCTGAC AATTTCTTTC 
TCTCGATGCT GGGAATTTCT TCTCTGGATG GATCCATCTA ACTATGGCGG AATCAAAGGA 
AAAGTTTCAT CTCGTATTCA CTGTGGACTG CAAGACTCCC AGAAAGCAGG GGGAGCAGAG 
GATGAGCAGG AAAATGAGGA CGATGAGGAG GAAAGAGATG GGGAGGAGGA GGAAGAGGCG 
GAGGAATCCA ACGTGGAGGA TTTACTGGAA AACAACTGGA ACATTTTGCA GTTTTTGCCA 
CAGGCAGCCT CCTGCCAGAA CTACTTCCTC ATGATTGTTT CAGCGTACAT CGTGGCCGTG 
TACCACTGCA TGAAGGACGG GCTGAGGCGC AGTGCTCCAG GGAGCACCCC CGTGAGGAGG 
AGGGGGGCCA GCCAGCTCTC CCAGGAGGCC GAGGGGGCGG TCGGAGCCCT TCCCGGAATG 
ATCACCTTCT CTCAGGATTA TGTCGCAAAT GAGCTCACTC AGAGCTTCTT CACCATCACT 
CAGAAGATTC AGAAGAAAGT CACAGGCTCT CGGAACTCCA CTGAGCTCTC AGAGATGTTT 
CCTGTCCTCC CCGGTTCCCA CTTGCTGCTC AATAACCCTG CCCTGGAGTT CATCAAATAC 
GTGTGCAAGG TGCTGTCCCT GGACACCAAC ATCACAAACC AGGTGAATAA GCTGAACCGA 
GACCTGCTTC GCCTGGTGGA TGTCGGCGAG TTCTCCGAGG AGGCCCAGTT CCGAGACCCC 
TGCCGCTCCT ACGTGCTTCC TGAGGTCATC TGCCGCAGCT GTAACTTCTG CCGCGACCTG 
GACCTGTGTA AAGACTCTTC CTTCTCAGAG GATGGGGCGG TCCTGCCTCA GTGGCTCTGC 
TCCAACTGTC AGGCGCCCTA CGACTCCTCT GCCATCGAGA TGACGCTGGT GGAAGTTCTA 
CAGAAGAAGC TGATGGCCTT CACCCTGCAG GACCTGCGGA AGGAGGCAGA CACTCCCAAG 
TGTGAAGAGA AGGAAAGGAG GAAGAGGGAG TTGCACAGCT CGGCCACAGG AAGGTCTTGC 
CGAGGGGCAT CTGAGCAGAG GCTGGGGGAC GCGCCAAGCT GCCGTGTGGT GGTGCACGGC 
GTTCAGGCCT GCAGGATGGA GGGAGTGAGC AGGGCCTGGA GGAGGCCTCA GGACTGGGCA 
TCTGGGGTGG AACATCCACA GCGAGGGTGG GTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_011534798.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533100.1 Homo sapiens polymerase (DNA) epsilon, catalytic subunit (POLE), transcript variant X3, mRNA.

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CloneID	OHu59811
Clone ID Related Accession (Same CDS sequence)	XM_011534798.1
Accession Version	XM_011534798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DNA polymerase epsilon catalytic subunit A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGG AGCAGCCCCA GACTCTGGCC ACGTATTCTG TGTCAGATGC TGTCGCCACT 
TACTACCTGT ACATGAAGTA CGTCCACCCA TTCATCTTTG CTCTGTGCAC CATTATTCCC 
ATGGAGCCCG ACGAGGTGCT GCGGAAGGGC TCTGGCACTC TGTGTGAGGC CTTGCTGATG 
GTGCAGGCCT TCCACGCCAA CATCATCTTC CCCAACAAGC AAGAGCAGGA GTTCAATAAG 
CTGACGGACG ACGGACACGT GCTGGACTCT GAGACCTACG TCGGGGGCCA CGTGGAGGCC 
CTCGAGTCTG GGGTTTTCCG CAGCGATATC CCTTGCCGGT TTAGGATGAA TCCTGCCGCC 
TTTGACTTCC TGCTGCAGCG GGTTGAGAAG ACCTTGCGCC ACGCCCTTGA GGAAGAGGAG 
AAAGTGCCTG TGGAGCAAGT CACCAACTTT GAAGAGGTGT GTGATGAGAT TAAGAGCAAG 
CTTGCCTCCC TGAAGGACGT TCCCAGCCGC ATCGAGTGTC CACTCATCTA CCACCTGGAC 
GTGGGGGCCA TGTACCCCAA CATCATCCTG ACCAACCGCC TGCAGCCCTC TGCCATGGTG 
GACGAAGCCA CCTGTGCTGC CTGTGACTTC AATAAGCCTG GAGCAAACTG CCAGCGGAAG 
ATGGCCTGGC AGTGGAGGGG CGAGTTCATG CCAGCCAGTC GCAGCGAATA CCATCGGATC 
CAGCACCAGC TGGAGTCAGA GAAGTTCCCC CCCTTGTTCC CAGAGGGGCC AGCTCGGGCC 
TTTCATGAAC TGTCCCGCGA GGAACAGGCG AAATACGAGA AGAGAAGGCT GGCGGATTAC 
TGCCGGAAAG CCTACAAGAA GATCCACATC ACCAAGGTGG AAGAGCGTCT CACCACCATC 
TGCCAGCGGG AAAACTCCTT CTACGTGGAC ACCGTGCGTG CCTTCCGGGA CAGGCGTTAC 
GAGTTCAAAG GGCTCCACAA GGTGTGGAAA AAGAAGCTCT CGGCGGCCGT GGAGGTGGGC 
GACGCGGCTG AGGTGAAGCG CTGCAAGAAC ATGGAGGTGC TGTATGACTC GCTGCAGCTG 
GCCCACAAGT GCATCCTGAA CTCCTTCTAT GGCTATGTCA TGCGCAAGGG GGCTCGCTGG 
TACTCCATGG AGATGGCTGG CATCGTCTGC TTCACAGGGG CCAACATCAT CACCCAGGCA 
CGGGAGCTGA TCGAGCAGAT TGGGAGGCCC TTAGAGCTGG ACACAGATGG TATATGGTGC 
GTCCTGCCCA ACAGCTTCCC AGAAAATTTT GTCTTCAAGA CGACCAATGT GAAGAAGCCC 
AAAGTGACCA TCTCCTACCC AGGCGCCATG TTGAACATCA TGGTCAAGGA AGGCTTCACC 
AATGACCAGT ACCAGGAGCT GGCTGAGCCG TCCTCACTCA CCTACGTCAC CCGCTCAGAG 
AACAGCATCT TTTTTGAGGT TGATGGGCCC TACCTTGCCA TGATTCTTCC AGCCTCCAAG 
GAAGAAGGCA AGAAATTGAA GAAGAGGTAT GCTGTGTTCA ATGAAGACGG TTCTCTGGCT 
GAGCTCAAGG GCTTTGAGGT CAAACGCCGC GGGGAACTGC AGCTGATTAA GATCTTCCAA 
TCCTCGGTGT TTGAGGCCTT CCTCAAGGGC AGCACGCTGG AAGAGGTGTA TGGCTCTGTA 
GCCAAGGTGG CTGACTACTG GCTGGACGTG CTGTACAGCA AGGCAGCCAA CATGCCTGAC 
TCTGAGCTAT TCGAGCTCAT CTCTGAGAAC CGTTCCATGT CTCGGAAGCT GGAAGATTAC 
GGGGAGCAGA AGTCTACGTC CATCAGCACA GCAAAGCGCC TGGCCGAGTT CCTGGGAGAC 
CAGATGGTCA AGGATGCAGG GCTGAGTTGC CGCTACATCA TCTCCCGCAA GCCCGAGGGC 
TCCCCTGTCA CGGAGAGGGC CATCCCACTT GCCATTTTCC AAGCAGAGCC CACGGTGAGG 
AAGCACTTTC TCCGGAAATG GCTCAAGAGC TCTTCCCTTC AAGACTTTGA TATTCGAGCA 
ATTCTGGATT GGGACTACTA CATTGAGCGG CTGGGAAGCG CCATCCAGAA GATCATCACC 
ATCCCTGCGG CCCTGCAGCA GGTAAAGAAC CCAGTGCCAC GTGTCAAACA CCCCGACTGG 
CTGCACAAAA AACTGCTGGA GAAGAATGAT GTCTACAAGC AGAAGAAGAT CAGTGAGCTC 
TTCACCCTGG AGGGCAGGAG ACAGGTCACG ATGGCCGAGG CCTCAGAAGA CAGTCCGAGG 
CCAAGTGCTC CTGACATGGA GGACTTCGGC CTCGTAAAGC TGCCTCACCC AGCAGCCCCT 
GTCACTGTGA AGAGGAAGCG AGTTCTTTGG GAGAGCCAGG AGGAGTCCCA GGACCTCACG 
CCGACTGTGC CCTGGCAGGA AATCTTGGGG CAGCCTCCCG CCCTGGGAAC CAGCCAGGAG 
GAATGGCTTG TCTGGCTCCG GTTCCACAAG AAGAAGTGGC AGCTGCAGGC CCGGCAGCGC 
CTCGCCCGCA GGAAGAGGCA GCGTCTGGAG TCGGCAGAGG GTGTGCTCAG GCCCGGGGCC 
ATCCGGGATG GTCCTGCCAC GGGGCTGGGG AGCTTCTTGC GAAGAACTGC CCGCAGCATC 
CTGGACCTTC CGTGGCAGAT TGTGCAGATC AGCGAGACCA GCCAGGCCGG CCTGTTCAGG 
CTGTGGGCGC TCGTTGGCAG TGACTTGCAC TGCATCAGGC TGAGCATCCC CCGTGTGTTC 
TACGTGAACC AGCGAGTCGC TAAAGCGGAG GAGGGTGCTT CGTATCGCAA GGTAAATCGG 
GTCCTTCCTC GCTCCAACAT GGTCTACAAT CTCTATGAGT ATTCAGTGCC AGAGGACATG 
TACCAGGAAC ACATCAACGA GATCAACGCT GAGCTGTCAG CGCCAGACAT CGAGGGCGTA 
TATGAGACTC AGGTTCCGTT ACTGTTCCGG GCCCTGGTGC ACCTGGGCTG TGTGTGTGTG 
GTCAATAAAC AGCTGGTGAG GCACCTTTCA GGCTGGGAAG CAGAGACCTT TGCTCTTGAG 
CACCTGGAGA TGCGCTCTCT GGCCCAGTTC AGCTACCTGG AACCAGGGAG TATCCGCCAT 
ATCTACCTGT ACCACCACGC ACAGGCCCAC AAAGCGCTCT TCGGGATCTT CATCCCCTCA 
CAGCGCAGGG CATCCGTCTT TGTGCTGGAC ACTGTGCGCA GCAACCAGAT GCCCAGCCTT 
GGCGCCCTGT ACTCAGCAGA GCACGGCCTC CTCCTGGAGA AGGTGGGCCC TGAGCTCCTG 
CCACCCCCCA AACACACCTT CGAAGTTCGG GCAGAAACTG ACCTGAAGAC CATCTGCAGA 
GCCATCCAGC GATTCCTGCT CGCCTACAAG GAGGAGCGCC GGGGGCCCAC ACTCATCGCT 
GTTCAGTCCA GCTGGGAGCT GAAGAGGCTG GCCAGTGAAA TTCCTGTCTT GGAGGAATTC 
CCACTGGTGC CTATCTGTGT GGCTGACAAG ATCAACTATG GGGTCCTGGA CTGGCAGCGC 
CATGGAGCCC GGCGCATGAT CCGTCACTAC CTCAACCTGG ACACCTGCCT GTCGCAGGCC 
TTCGAGATGA GCAGGTACTT TCACATTCCC ATTGGGAACC TACCAGAGGA CATCTCCACA 
TTCGGCTCCG ACCTCTTCTT TGCCCGCCAC CTCCAGCGCC ACAACCACCT GCTCTGGCTG 
TCCCCTACAG CCCGCCCTGA CCTGGGTGGA AAGGAGGCTG ATGACAACTG TCTTGTCATG 
GAGTTCGATG ACCAAGCCAC TGTTGAGATC AACAGTTCAG GCTGTTACTC CACAGTGTGT 
GTGGAGCTGG ACCTTCAGAA CCTGGCCGTC AACACCATTC TCCAGTCTCA CCATGTCAAC 
GACATGGAGG GGGCCGACAG CATGGGGATC AGCTTCGACG TGATCCAGCA GGCCTCCCTG 
GAGGACATGA TCACGGGTGG TCAGGCTGCC AGTGCCCCGG CCAGCTACGA TGAGACAGCC 
CTGTGCTCTA ACACCTTCAG GATCCTGAAG AGCATGGTCG TGGGCTGGGT GAAGGAGATC 
ACCCAGTACC ACAACATCTA TGCAGACAAC CAGGTGATGC ACTTCTACCG CTGGCTTCGG 
TCGCCATCCT CTCTGCTTCA TGACCCTGCC CTGCACCGCA CACTCCACAA CATGATGAAG 
AAGCTCTTCC TGCAGCTCAT CGCTGAGTTC AAGCGCCTGG GGTCATCAGT CATCTACGCC 
AACTTCAACC GCATCATCCT CTGTACAAAG AAGCGCCGTG TGGAAGATGC CATCGCTTAC 
GTGGAGTACA TCACCAGCAG CATCCATTCA AAGGAGACCT TCCATTCTCT GACAATTTCT 
TTCTCTCGAT GCTGGGAATT TCTTCTCTGG ATGGATCCAT CTAACTATGG CGGAATCAAA 
GGAAAAGTTT CATCTCGTAT TCACTGTGGA CTGCAAGACT CCCAGAAAGC AGGGGGAGCA 
GAGGATGAGC AGGAAAATGA GGACGATGAG GAGGAAAGAG ATGGGGAGGA GGAGGAAGAG 
GCGGAGGAAT CCAACGTGGA GGATTTACTG GAAAACAACT GGAACATTTT GCAGTTTTTG 
CCACAGGCAG CCTCCTGCCA GAACTACTTC CTCATGATTG TTTCAGCGTA CATCGTGGCC 
GTGTACCACT GCATGAAGGA CGGGCTGAGG CGCAGTGCTC CAGGGAGCAC CCCCGTGAGG 
AGGAGGGGGG CCAGCCAGCT CTCCCAGGAG GCCGAGGGGG CGGTCGGAGC CCTTCCCGGA 
ATGATCACCT TCTCTCAGGA TTATGTCGCA AATGAGCTCA CTCAGAGCTT CTTCACCATC 
ACTCAGAAGA TTCAGAAGAA AGTCACAGGC TCTCGGAACT CCACTGAGCT CTCAGAGATG 
TTTCCTGTCC TCCCCGGTTC CCACTTGCTG CTCAATAACC CTGCCCTGGA GTTCATCAAA 
TACGTGTGCA AGGTGCTGTC CCTGGACACC AACATCACAA ACCAGGTGAA TAAGCTGAAC 
CGAGACCTGC TTCGCCTGGT GGATGTCGGC GAGTTCTCCG AGGAGGCCCA GTTCCGAGAC 
CCCTGCCGCT CCTACGTGCT TCCTGAGGTC ATCTGCCGCA GCTGTAACTT CTGCCGCGAC 
CTGGACCTGT GTAAAGACTC TTCCTTCTCA GAGGATGGGG CGGTCCTGCC TCAGTGGCTC 
TGCTCCAACT GTCAGGCGCC CTACGACTCC TCTGCCATCG AGATGACGCT GGTGGAAGTT 
CTACAGAAGA AGCTGATGGC CTTCACCCTG CAGGACCTGC GGAAGGAGGC AGACACTCCC 
AAGTGTGAAG AGAAGGAAAG GAGGAAGAGG GAGTTGCACA GCTCGGCCAC AGGAAGGTCT 
TGCCGAGGGG CATCTGAGCA GAGGCTGGGG GACGCGCCAA GCTGCCGTGT GGTGGTGCAC 
GGCGTTCAGG CCTGCAGGAT GGAGGGAGTG AGCAGGGCCT GGAGGAGGCC TCAGGACTGG 
GCATCTGGGG TGGAACATCC ACAGCGAGGG TGGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533100.1
CDS	64..5622
Translation	MATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDSETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTLRHALEEEEKVPVEQVTNFEEVCDEIKSKLASLKDVPSRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHITKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVFKTTNVKKPKVTISYPGAMLNIMVKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRAILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFTLEGRRQVTMAEASEDSPRPSAPDMEDFGLVKLPHPAAPVTVKRKRVLWESQEESQDLTPTVPWQEILGQPPALGTSQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEGVLRPGAIRDGPATGLGSFLRRTARSILDLPWQIVQISETSQAGLFRLWALVGSDLHCIRLSIPRVFYVNQRVAKAEEGASYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINAELSAPDIEGVYETQVPLLFRALVHLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHAQAHKALFGIFIPSQRRASVFVLDTVRSNQMPSLGALYSAEHGLLLEKVGPELLPPPKHTFEVRAETDLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELKRLASEIPVLEEFPLVPICVADKINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTARPDLGGKEADDNCLVMEFDDQATVEINSSGCYSTVCVELDLQNLAVNTILQSHHVNDMEGADSMGISFDVIQQASLEDMITGGQAASAPASYDETALCSNTFRILKSMVVGWVKEITQYHNIYADNQVMHFYRWLRSPSSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVIYANFNRIILCTKKRRVEDAIAYVEYITSSIHSKETFHSLTISFSRCWEFLLWMDPSNYGGIKGKVSSRIHCGLQDSQKAGGAEDEQENEDDEEERDGEEEEEAEESNVEDLLENNWNILQFLPQAASCQNYFLMIVSAYIVAVYHCMKDGLRRSAPGSTPVRRRGASQLSQEAEGAVGALPGMITFSQDYVANELTQSFFTITQKIQKKVTGSRNSTELSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCKDSSFSEDGAVLPQWLCSNCQAPYDSSAIEMTLVEVLQKKLMAFTLQDLRKEADTPKCEEKERRKRELHSSATGRSCRGASEQRLGDAPSCRVVVHGVQACRMEGVSRAWRRPQDWASGVEHPQRGWV

Target ORF information:

RefSeq Version	XM_011534798.1
Organism	Homo sapiens(human)
Definition	Homo sapiens polymerase (DNA) epsilon, catalytic subunit (POLE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534798.1

ORF Insert Sequence:

ATGGCCACGG AGCAGCCCCA GACTCTGGCC ACGTATTCTG TGTCAGATGC TGTCGCCACT 
TACTACCTGT ACATGAAGTA CGTCCACCCA TTCATCTTTG CTCTGTGCAC CATTATTCCC 
ATGGAGCCCG ACGAGGTGCT GCGGAAGGGC TCTGGCACTC TGTGTGAGGC CTTGCTGATG 
GTGCAGGCCT TCCACGCCAA CATCATCTTC CCCAACAAGC AAGAGCAGGA GTTCAATAAG 
CTGACGGACG ACGGACACGT GCTGGACTCT GAGACCTACG TCGGGGGCCA CGTGGAGGCC 
CTCGAGTCTG GGGTTTTCCG CAGCGATATC CCTTGCCGGT TTAGGATGAA TCCTGCCGCC 
TTTGACTTCC TGCTGCAGCG GGTTGAGAAG ACCTTGCGCC ACGCCCTTGA GGAAGAGGAG 
AAAGTGCCTG TGGAGCAAGT CACCAACTTT GAAGAGGTGT GTGATGAGAT TAAGAGCAAG 
CTTGCCTCCC TGAAGGACGT TCCCAGCCGC ATCGAGTGTC CACTCATCTA CCACCTGGAC 
GTGGGGGCCA TGTACCCCAA CATCATCCTG ACCAACCGCC TGCAGCCCTC TGCCATGGTG 
GACGAAGCCA CCTGTGCTGC CTGTGACTTC AATAAGCCTG GAGCAAACTG CCAGCGGAAG 
ATGGCCTGGC AGTGGAGGGG CGAGTTCATG CCAGCCAGTC GCAGCGAATA CCATCGGATC 
CAGCACCAGC TGGAGTCAGA GAAGTTCCCC CCCTTGTTCC CAGAGGGGCC AGCTCGGGCC 
TTTCATGAAC TGTCCCGCGA GGAACAGGCG AAATACGAGA AGAGAAGGCT GGCGGATTAC 
TGCCGGAAAG CCTACAAGAA GATCCACATC ACCAAGGTGG AAGAGCGTCT CACCACCATC 
TGCCAGCGGG AAAACTCCTT CTACGTGGAC ACCGTGCGTG CCTTCCGGGA CAGGCGTTAC 
GAGTTCAAAG GGCTCCACAA GGTGTGGAAA AAGAAGCTCT CGGCGGCCGT GGAGGTGGGC 
GACGCGGCTG AGGTGAAGCG CTGCAAGAAC ATGGAGGTGC TGTATGACTC GCTGCAGCTG 
GCCCACAAGT GCATCCTGAA CTCCTTCTAT GGCTATGTCA TGCGCAAGGG GGCTCGCTGG 
TACTCCATGG AGATGGCTGG CATCGTCTGC TTCACAGGGG CCAACATCAT CACCCAGGCA 
CGGGAGCTGA TCGAGCAGAT TGGGAGGCCC TTAGAGCTGG ACACAGATGG TATATGGTGC 
GTCCTGCCCA ACAGCTTCCC AGAAAATTTT GTCTTCAAGA CGACCAATGT GAAGAAGCCC 
AAAGTGACCA TCTCCTACCC AGGCGCCATG TTGAACATCA TGGTCAAGGA AGGCTTCACC 
AATGACCAGT ACCAGGAGCT GGCTGAGCCG TCCTCACTCA CCTACGTCAC CCGCTCAGAG 
AACAGCATCT TTTTTGAGGT TGATGGGCCC TACCTTGCCA TGATTCTTCC AGCCTCCAAG 
GAAGAAGGCA AGAAATTGAA GAAGAGGTAT GCTGTGTTCA ATGAAGACGG TTCTCTGGCT 
GAGCTCAAGG GCTTTGAGGT CAAACGCCGC GGGGAACTGC AGCTGATTAA GATCTTCCAA 
TCCTCGGTGT TTGAGGCCTT CCTCAAGGGC AGCACGCTGG AAGAGGTGTA TGGCTCTGTA 
GCCAAGGTGG CTGACTACTG GCTGGACGTG CTGTACAGCA AGGCAGCCAA CATGCCTGAC 
TCTGAGCTAT TCGAGCTCAT CTCTGAGAAC CGTTCCATGT CTCGGAAGCT GGAAGATTAC 
GGGGAGCAGA AGTCTACGTC CATCAGCACA GCAAAGCGCC TGGCCGAGTT CCTGGGAGAC 
CAGATGGTCA AGGATGCAGG GCTGAGTTGC CGCTACATCA TCTCCCGCAA GCCCGAGGGC 
TCCCCTGTCA CGGAGAGGGC CATCCCACTT GCCATTTTCC AAGCAGAGCC CACGGTGAGG 
AAGCACTTTC TCCGGAAATG GCTCAAGAGC TCTTCCCTTC AAGACTTTGA TATTCGAGCA 
ATTCTGGATT GGGACTACTA CATTGAGCGG CTGGGAAGCG CCATCCAGAA GATCATCACC 
ATCCCTGCGG CCCTGCAGCA GGTAAAGAAC CCAGTGCCAC GTGTCAAACA CCCCGACTGG 
CTGCACAAAA AACTGCTGGA GAAGAATGAT GTCTACAAGC AGAAGAAGAT CAGTGAGCTC 
TTCACCCTGG AGGGCAGGAG ACAGGTCACG ATGGCCGAGG CCTCAGAAGA CAGTCCGAGG 
CCAAGTGCTC CTGACATGGA GGACTTCGGC CTCGTAAAGC TGCCTCACCC AGCAGCCCCT 
GTCACTGTGA AGAGGAAGCG AGTTCTTTGG GAGAGCCAGG AGGAGTCCCA GGACCTCACG 
CCGACTGTGC CCTGGCAGGA AATCTTGGGG CAGCCTCCCG CCCTGGGAAC CAGCCAGGAG 
GAATGGCTTG TCTGGCTCCG GTTCCACAAG AAGAAGTGGC AGCTGCAGGC CCGGCAGCGC 
CTCGCCCGCA GGAAGAGGCA GCGTCTGGAG TCGGCAGAGG GTGTGCTCAG GCCCGGGGCC 
ATCCGGGATG GTCCTGCCAC GGGGCTGGGG AGCTTCTTGC GAAGAACTGC CCGCAGCATC 
CTGGACCTTC CGTGGCAGAT TGTGCAGATC AGCGAGACCA GCCAGGCCGG CCTGTTCAGG 
CTGTGGGCGC TCGTTGGCAG TGACTTGCAC TGCATCAGGC TGAGCATCCC CCGTGTGTTC 
TACGTGAACC AGCGAGTCGC TAAAGCGGAG GAGGGTGCTT CGTATCGCAA GGTAAATCGG 
GTCCTTCCTC GCTCCAACAT GGTCTACAAT CTCTATGAGT ATTCAGTGCC AGAGGACATG 
TACCAGGAAC ACATCAACGA GATCAACGCT GAGCTGTCAG CGCCAGACAT CGAGGGCGTA 
TATGAGACTC AGGTTCCGTT ACTGTTCCGG GCCCTGGTGC ACCTGGGCTG TGTGTGTGTG 
GTCAATAAAC AGCTGGTGAG GCACCTTTCA GGCTGGGAAG CAGAGACCTT TGCTCTTGAG 
CACCTGGAGA TGCGCTCTCT GGCCCAGTTC AGCTACCTGG AACCAGGGAG TATCCGCCAT 
ATCTACCTGT ACCACCACGC ACAGGCCCAC AAAGCGCTCT TCGGGATCTT CATCCCCTCA 
CAGCGCAGGG CATCCGTCTT TGTGCTGGAC ACTGTGCGCA GCAACCAGAT GCCCAGCCTT 
GGCGCCCTGT ACTCAGCAGA GCACGGCCTC CTCCTGGAGA AGGTGGGCCC TGAGCTCCTG 
CCACCCCCCA AACACACCTT CGAAGTTCGG GCAGAAACTG ACCTGAAGAC CATCTGCAGA 
GCCATCCAGC GATTCCTGCT CGCCTACAAG GAGGAGCGCC GGGGGCCCAC ACTCATCGCT 
GTTCAGTCCA GCTGGGAGCT GAAGAGGCTG GCCAGTGAAA TTCCTGTCTT GGAGGAATTC 
CCACTGGTGC CTATCTGTGT GGCTGACAAG ATCAACTATG GGGTCCTGGA CTGGCAGCGC 
CATGGAGCCC GGCGCATGAT CCGTCACTAC CTCAACCTGG ACACCTGCCT GTCGCAGGCC 
TTCGAGATGA GCAGGTACTT TCACATTCCC ATTGGGAACC TACCAGAGGA CATCTCCACA 
TTCGGCTCCG ACCTCTTCTT TGCCCGCCAC CTCCAGCGCC ACAACCACCT GCTCTGGCTG 
TCCCCTACAG CCCGCCCTGA CCTGGGTGGA AAGGAGGCTG ATGACAACTG TCTTGTCATG 
GAGTTCGATG ACCAAGCCAC TGTTGAGATC AACAGTTCAG GCTGTTACTC CACAGTGTGT 
GTGGAGCTGG ACCTTCAGAA CCTGGCCGTC AACACCATTC TCCAGTCTCA CCATGTCAAC 
GACATGGAGG GGGCCGACAG CATGGGGATC AGCTTCGACG TGATCCAGCA GGCCTCCCTG 
GAGGACATGA TCACGGGTGG TCAGGCTGCC AGTGCCCCGG CCAGCTACGA TGAGACAGCC 
CTGTGCTCTA ACACCTTCAG GATCCTGAAG AGCATGGTCG TGGGCTGGGT GAAGGAGATC 
ACCCAGTACC ACAACATCTA TGCAGACAAC CAGGTGATGC ACTTCTACCG CTGGCTTCGG 
TCGCCATCCT CTCTGCTTCA TGACCCTGCC CTGCACCGCA CACTCCACAA CATGATGAAG 
AAGCTCTTCC TGCAGCTCAT CGCTGAGTTC AAGCGCCTGG GGTCATCAGT CATCTACGCC 
AACTTCAACC GCATCATCCT CTGTACAAAG AAGCGCCGTG TGGAAGATGC CATCGCTTAC 
GTGGAGTACA TCACCAGCAG CATCCATTCA AAGGAGACCT TCCATTCTCT GACAATTTCT 
TTCTCTCGAT GCTGGGAATT TCTTCTCTGG ATGGATCCAT CTAACTATGG CGGAATCAAA 
GGAAAAGTTT CATCTCGTAT TCACTGTGGA CTGCAAGACT CCCAGAAAGC AGGGGGAGCA 
GAGGATGAGC AGGAAAATGA GGACGATGAG GAGGAAAGAG ATGGGGAGGA GGAGGAAGAG 
GCGGAGGAAT CCAACGTGGA GGATTTACTG GAAAACAACT GGAACATTTT GCAGTTTTTG 
CCACAGGCAG CCTCCTGCCA GAACTACTTC CTCATGATTG TTTCAGCGTA CATCGTGGCC 
GTGTACCACT GCATGAAGGA CGGGCTGAGG CGCAGTGCTC CAGGGAGCAC CCCCGTGAGG 
AGGAGGGGGG CCAGCCAGCT CTCCCAGGAG GCCGAGGGGG CGGTCGGAGC CCTTCCCGGA 
ATGATCACCT TCTCTCAGGA TTATGTCGCA AATGAGCTCA CTCAGAGCTT CTTCACCATC 
ACTCAGAAGA TTCAGAAGAA AGTCACAGGC TCTCGGAACT CCACTGAGCT CTCAGAGATG 
TTTCCTGTCC TCCCCGGTTC CCACTTGCTG CTCAATAACC CTGCCCTGGA GTTCATCAAA 
TACGTGTGCA AGGTGCTGTC CCTGGACACC AACATCACAA ACCAGGTGAA TAAGCTGAAC 
CGAGACCTGC TTCGCCTGGT GGATGTCGGC GAGTTCTCCG AGGAGGCCCA GTTCCGAGAC 
CCCTGCCGCT CCTACGTGCT TCCTGAGGTC ATCTGCCGCA GCTGTAACTT CTGCCGCGAC 
CTGGACCTGT GTAAAGACTC TTCCTTCTCA GAGGATGGGG CGGTCCTGCC TCAGTGGCTC 
TGCTCCAACT GTCAGGCGCC CTACGACTCC TCTGCCATCG AGATGACGCT GGTGGAAGTT 
CTACAGAAGA AGCTGATGGC CTTCACCCTG CAGGACCTGC GGAAGGAGGC AGACACTCCC 
AAGTGTGAAG AGAAGGAAAG GAGGAAGAGG GAGTTGCACA GCTCGGCCAC AGGAAGGTCT 
TGCCGAGGGG CATCTGAGCA GAGGCTGGGG GACGCGCCAA GCTGCCGTGT GGTGGTGCAC 
GGCGTTCAGG CCTGCAGGAT GGAGGGAGTG AGCAGGGCCT GGAGGAGGCC TCAGGACTGG 
GCATCTGGGG TGGAACATCC ACAGCGAGGG TGGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_011534799.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533101.1 Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X4, mRNA.

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CloneID	OHu59812
Clone ID Related Accession (Same CDS sequence)	XM_011534799.2
Accession Version	XM_011534799.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA polymerase epsilon catalytic subunit A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534799.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG 
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT 
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT 
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC 
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC 
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG 
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC 
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC 
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG 
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA 
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA 
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC 
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG 
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC 
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT 
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC 
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG 
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT 
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG 
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA 
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC 
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT 
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC 
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT 
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG 
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC 
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA 
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG 
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC 
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG 
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG 
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG 
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG 
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC 
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT 
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG 
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA 
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG 
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC 
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA 
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA 
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC 
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT 
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC 
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG 
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG 
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC 
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT 
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA 
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC 
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG 
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG 
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT 
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG 
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC 
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA 
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC 
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC 
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG 
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC 
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG 
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT 
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG 
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC 
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA 
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT 
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC 
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC 
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC 
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGG CCACTTTTCG ATGTGACCAA 
AGGTACATCA CAGGGATCTC CAGCCAGCTC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533101.1
CDS	205..5640
Translation	MSLRSGGRRRADPGADGEASRDDGATSSVSALKRLERSQWTDKMDLRFGFERLKEPGEKTGWLINMHPTEILDEDKRLGSAVDYYFIQDDGSRFKVALPYKPYFYIATRKGCEREVSSFLSKKFQGKIAKVETVPKEDLDLPNHLVGLKRNYIRLSFHTVEDLVKVRKEISPAVKKNREQDHASDAYTALLSSVLQRGGVITDEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNVRYRGNAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEAHLIQRWFEHVQETKPTIMVTYNGDFFDWPFVEARAAVHGLSMQQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDSETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTLRHALEEEEKVPVEQVTNFEEVCDEIKSKLASLKDVPSRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHITKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVFKTTNVKKPKVTISYPGAMLNIMVKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRAILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFTLEGRRQVTMAEASEDSPRPSAPDMEDFGLVKLPHPAAPVTVKRKRVLWESQEESQDLTPTVPWQEILGQPPALGTSQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEGVLRPGAIRDGPATGLGSFLRRTARSILDLPWQIVQISETSQAGLFRLWALVGSDLHCIRLSIPRVFYVNQRVAKAEEGASYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINAELSAPDIEGVYETQVPLLFRALVHLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHAQAHKALFGIFIPSQRRASVFVLDTVRSNQMPSLGALYSAEHGLLLEKVGPELLPPPKHTFEVRAETDLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELKRLASEIPVLEEFPLVPICVADKINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTARPDLGGKEADDNCLVMEFDDQATVEINSSGCYSTVCVELDLQNLAVNTILQSHHVNDMEGADSMGISFDVIQQASLEDMITGGQAASAPASYDETALCSNTFRATFRCDQRYITGISSQLY

Target ORF information:

RefSeq Version	XM_011534799.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534799.2

ORF Insert Sequence:

ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG 
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT 
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT 
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC 
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC 
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG 
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC 
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC 
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG 
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA 
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA 
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC 
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG 
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC 
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT 
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC 
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG 
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT 
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG 
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA 
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC 
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT 
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC 
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT 
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG 
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC 
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA 
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG 
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC 
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG 
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG 
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG 
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG 
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC 
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT 
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG 
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA 
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG 
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC 
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA 
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA 
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC 
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT 
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC 
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG 
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG 
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC 
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT 
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA 
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC 
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG 
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG 
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT 
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG 
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC 
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA 
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC 
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC 
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG 
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC 
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG 
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT 
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG 
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC 
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA 
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT 
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC 
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC 
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC 
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGG CCACTTTTCG ATGTGACCAA 
AGGTACATCA CAGGGATCTC CAGCCAGCTC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_011534800.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533102.1 Homo sapiens polymerase (DNA) epsilon, catalytic subunit (POLE), transcript variant X5, mRNA.

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CloneID	OHu59813
Clone ID Related Accession (Same CDS sequence)	XM_011534800.2
Accession Version	XM_011534800.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DNA polymerase epsilon catalytic subunit A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534800.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG 
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT 
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT 
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC 
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC 
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG 
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC 
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC 
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG 
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA 
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA 
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC 
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG 
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC 
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT 
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC 
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG 
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT 
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG 
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA 
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC 
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT 
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC 
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT 
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG 
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC 
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA 
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG 
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC 
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG 
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG 
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG 
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG 
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC 
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT 
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG 
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA 
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG 
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC 
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA 
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA 
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC 
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT 
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC 
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG 
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG 
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC 
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT 
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA 
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC 
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG 
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG 
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT 
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG 
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC 
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA 
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC 
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC 
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG 
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC 
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG 
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT 
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG 
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC 
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA 
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT 
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC 
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC 
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC 
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGT ACATCACAGG GATCTCCAGC 
CAGCTCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533102.1
CDS	205..5616
Translation	MSLRSGGRRRADPGADGEASRDDGATSSVSALKRLERSQWTDKMDLRFGFERLKEPGEKTGWLINMHPTEILDEDKRLGSAVDYYFIQDDGSRFKVALPYKPYFYIATRKGCEREVSSFLSKKFQGKIAKVETVPKEDLDLPNHLVGLKRNYIRLSFHTVEDLVKVRKEISPAVKKNREQDHASDAYTALLSSVLQRGGVITDEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNVRYRGNAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEAHLIQRWFEHVQETKPTIMVTYNGDFFDWPFVEARAAVHGLSMQQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDSETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTLRHALEEEEKVPVEQVTNFEEVCDEIKSKLASLKDVPSRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHITKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVFKTTNVKKPKVTISYPGAMLNIMVKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRAILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFTLEGRRQVTMAEASEDSPRPSAPDMEDFGLVKLPHPAAPVTVKRKRVLWESQEESQDLTPTVPWQEILGQPPALGTSQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEGVLRPGAIRDGPATGLGSFLRRTARSILDLPWQIVQISETSQAGLFRLWALVGSDLHCIRLSIPRVFYVNQRVAKAEEGASYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINAELSAPDIEGVYETQVPLLFRALVHLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHAQAHKALFGIFIPSQRRASVFVLDTVRSNQMPSLGALYSAEHGLLLEKVGPELLPPPKHTFEVRAETDLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELKRLASEIPVLEEFPLVPICVADKINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTARPDLGGKEADDNCLVMEFDDQATVEINSSGCYSTVCVELDLQNLAVNTILQSHHVNDMEGADSMGISFDVIQQASLEDMITGGQAASAPASYDETALCSNTFRYITGISSQLY

Target ORF information:

RefSeq Version	XM_011534800.2
Organism	Homo sapiens(human)
Definition	Homo sapiens polymerase (DNA) epsilon, catalytic subunit (POLE), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534800.2

ORF Insert Sequence:

ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG 
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT 
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT 
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC 
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC 
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG 
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC 
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC 
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG 
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA 
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA 
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC 
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG 
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC 
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT 
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC 
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG 
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT 
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG 
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA 
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC 
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT 
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC 
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT 
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG 
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC 
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA 
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG 
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC 
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG 
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG 
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG 
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG 
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC 
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT 
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG 
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA 
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG 
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC 
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA 
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA 
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC 
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT 
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC 
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG 
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG 
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC 
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT 
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA 
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC 
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG 
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG 
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT 
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG 
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC 
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA 
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC 
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC 
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG 
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC 
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG 
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT 
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG 
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC 
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA 
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT 
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC 
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC 
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC 
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGT ACATCACAGG GATCTCCAGC 
CAGCTCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( NM_006231.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006222.2 Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), mRNA.

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CloneID	OHu20900
Clone ID Related Accession (Same CDS sequence)	NM_006231.3 , NM_006231.4
Accession Version	NM_006231.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	DNA polymerase epsilon catalytic subunit A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L09561.1, CX782956.1 and BC011376.1. On Aug 31, 2019 this sequence version replaced NM_006231.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L09561.1, U49356.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG 
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT 
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT 
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC 
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC 
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG 
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC 
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC 
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG 
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA 
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA 
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC 
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG 
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC 
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT 
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC 
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG 
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT 
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG 
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA 
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC 
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT 
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC 
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT 
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG 
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC 
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA 
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG 
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC 
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG 
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG 
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG 
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG 
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC 
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT 
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG 
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA 
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG 
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC 
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA 
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA 
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC 
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT 
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC 
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG 
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG 
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC 
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT 
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA 
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC 
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG 
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG 
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT 
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG 
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC 
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA 
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC 
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC 
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG 
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC 
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG 
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT 
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG 
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC 
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA 
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT 
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC 
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC 
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC 
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGA TCCTGAAGAG CATGGTCGTG 
GGCTGGGTGA AGGAGATCAC CCAGTACCAC AACATCTATG CAGACAACCA GGTGATGCAC 
TTCTACCGCT GGCTTCGGTC GCCATCCTCT CTGCTTCATG ACCCTGCCCT GCACCGCACA 
CTCCACAACA TGATGAAGAA GCTCTTCCTG CAGCTCATCG CTGAGTTCAA GCGCCTGGGG 
TCATCAGTCA TCTACGCCAA CTTCAACCGC ATCATCCTCT GTACAAAGAA GCGCCGTGTG 
GAAGATGCCA TCGCTTACGT GGAGTACATC ACCAGCAGCA TCCATTCAAA GGAGACCTTC 
CATTCTCTGA CAATTTCTTT CTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCATCT 
AACTATGGCG GAATCAAAGG AAAAGTTTCA TCTCGTATTC ACTGTGGACT GCAAGACTCC 
CAGAAAGCAG GGGGAGCAGA GGATGAGCAG GAAAATGAGG ACGATGAGGA GGAAAGAGAT 
GGGGAGGAGG AGGAAGAGGC GGAGGAATCC AACGTGGAGG ATTTACTGGA AAACAACTGG 
AACATTTTGC AGTTTTTGCC ACAGGCAGCC TCCTGCCAGA ACTACTTCCT CATGATTGTT 
TCAGCGTACA TCGTGGCCGT GTACCACTGC ATGAAGGACG GGCTGAGGCG CAGTGCTCCA 
GGGAGCACCC CCGTGAGGAG GAGGGGGGCC AGCCAGCTCT CCCAGGAGGC CGAGGGGGCG 
GTCGGAGCCC TTCCCGGAAT GATCACCTTC TCTCAGGATT ATGTCGCAAA TGAGCTCACT 
CAGAGCTTCT TCACCATCAC TCAGAAGATT CAGAAGAAAG TCACAGGCTC TCGGAACTCC 
ACTGAGCTCT CAGAGATGTT TCCTGTCCTC CCCGGTTCCC ACTTGCTGCT CAATAACCCT 
GCCCTGGAGT TCATCAAATA CGTGTGCAAG GTGCTGTCCC TGGACACCAA CATCACAAAC 
CAGGTGAATA AGCTGAACCG AGACCTGCTT CGCCTGGTGG ATGTCGGCGA GTTCTCCGAG 
GAGGCCCAGT TCCGAGACCC CTGCCGCTCC TACGTGCTTC CTGAGGTCAT CTGCCGCAGC 
TGTAACTTCT GCCGCGACCT GGACCTGTGT AAAGACTCTT CCTTCTCAGA GGATGGGGCG 
GTCCTGCCTC AGTGGCTCTG CTCCAACTGT CAGGCGCCCT ACGACTCCTC TGCCATCGAG 
ATGACGCTGG TGGAAGTTCT ACAGAAGAAG CTGATGGCCT TCACCCTGCA GGACCTGGTC 
TGCCTGAAGT GCCGCGGGGT GAAGGAGACC AGCATGCCTG TGTACTGCAG CTGCGCGGGA 
GACTTCGCCC TCACCATCCA CACCCAGGTC TTCATGGAAC AGATCGGAAT ATTCCGGAAC 
ATTGCCCAGC ACTACGGCAT GTCGTACCTC CTGGAGACCC TGGAGTGGCT GCTGCAGAAG 
AACCCACAGC TGGGCCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006222.2
CDS	28..6888
Translation	MSLRSGGRRRADPGADGEASRDDGATSSVSALKRLERSQWTDKMDLRFGFERLKEPGEKTGWLINMHPTEILDEDKRLGSAVDYYFIQDDGSRFKVALPYKPYFYIATRKGCEREVSSFLSKKFQGKIAKVETVPKEDLDLPNHLVGLKRNYIRLSFHTVEDLVKVRKEISPAVKKNREQDHASDAYTALLSSVLQRGGVITDEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNVRYRGNAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEAHLIQRWFEHVQETKPTIMVTYNGDFFDWPFVEARAAVHGLSMQQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDSETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTLRHALEEEEKVPVEQVTNFEEVCDEIKSKLASLKDVPSRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHITKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVFKTTNVKKPKVTISYPGAMLNIMVKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRAILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFTLEGRRQVTMAEASEDSPRPSAPDMEDFGLVKLPHPAAPVTVKRKRVLWESQEESQDLTPTVPWQEILGQPPALGTSQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEGVLRPGAIRDGPATGLGSFLRRTARSILDLPWQIVQISETSQAGLFRLWALVGSDLHCIRLSIPRVFYVNQRVAKAEEGASYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINAELSAPDIEGVYETQVPLLFRALVHLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHAQAHKALFGIFIPSQRRASVFVLDTVRSNQMPSLGALYSAEHGLLLEKVGPELLPPPKHTFEVRAETDLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELKRLASEIPVLEEFPLVPICVADKINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTARPDLGGKEADDNCLVMEFDDQATVEINSSGCYSTVCVELDLQNLAVNTILQSHHVNDMEGADSMGISFDVIQQASLEDMITGGQAASAPASYDETALCSNTFRILKSMVVGWVKEITQYHNIYADNQVMHFYRWLRSPSSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVIYANFNRIILCTKKRRVEDAIAYVEYITSSIHSKETFHSLTISFSRCWEFLLWMDPSNYGGIKGKVSSRIHCGLQDSQKAGGAEDEQENEDDEEERDGEEEEEAEESNVEDLLENNWNILQFLPQAASCQNYFLMIVSAYIVAVYHCMKDGLRRSAPGSTPVRRRGASQLSQEAEGAVGALPGMITFSQDYVANELTQSFFTITQKIQKKVTGSRNSTELSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCKDSSFSEDGAVLPQWLCSNCQAPYDSSAIEMTLVEVLQKKLMAFTLQDLVCLKCRGVKETSMPVYCSCAGDFALTIHTQVFMEQIGIFRNIAQHYGMSYLLETLEWLLQKNPQLGH

Target ORF information:

RefSeq Version	NM_006231.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006231.4

ORF Insert Sequence:

ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG 
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT 
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT 
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC 
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC 
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG 
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC 
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC 
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG 
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA 
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA 
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC 
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG 
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC 
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT 
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC 
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG 
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT 
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG 
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA 
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC 
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT 
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC 
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT 
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG 
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC 
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA 
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG 
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC 
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG 
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG 
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG 
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG 
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC 
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT 
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG 
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA 
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG 
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC 
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA 
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA 
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC 
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT 
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC 
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG 
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG 
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC 
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT 
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA 
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC 
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG 
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG 
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT 
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG 
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC 
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA 
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC 
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC 
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG 
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC 
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG 
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT 
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG 
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC 
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA 
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT 
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC 
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC 
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC 
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGA TCCTGAAGAG CATGGTCGTG 
GGCTGGGTGA AGGAGATCAC CCAGTACCAC AACATCTATG CAGACAACCA GGTGATGCAC 
TTCTACCGCT GGCTTCGGTC GCCATCCTCT CTGCTTCATG ACCCTGCCCT GCACCGCACA 
CTCCACAACA TGATGAAGAA GCTCTTCCTG CAGCTCATCG CTGAGTTCAA GCGCCTGGGG 
TCATCAGTCA TCTACGCCAA CTTCAACCGC ATCATCCTCT GTACAAAGAA GCGCCGTGTG 
GAAGATGCCA TCGCTTACGT GGAGTACATC ACCAGCAGCA TCCATTCAAA GGAGACCTTC 
CATTCTCTGA CAATTTCTTT CTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCATCT 
AACTATGGCG GAATCAAAGG AAAAGTTTCA TCTCGTATTC ACTGTGGACT GCAAGACTCC 
CAGAAAGCAG GGGGAGCAGA GGATGAGCAG GAAAATGAGG ACGATGAGGA GGAAAGAGAT 
GGGGAGGAGG AGGAAGAGGC GGAGGAATCC AACGTGGAGG ATTTACTGGA AAACAACTGG 
AACATTTTGC AGTTTTTGCC ACAGGCAGCC TCCTGCCAGA ACTACTTCCT CATGATTGTT 
TCAGCGTACA TCGTGGCCGT GTACCACTGC ATGAAGGACG GGCTGAGGCG CAGTGCTCCA 
GGGAGCACCC CCGTGAGGAG GAGGGGGGCC AGCCAGCTCT CCCAGGAGGC CGAGGGGGCG 
GTCGGAGCCC TTCCCGGAAT GATCACCTTC TCTCAGGATT ATGTCGCAAA TGAGCTCACT 
CAGAGCTTCT TCACCATCAC TCAGAAGATT CAGAAGAAAG TCACAGGCTC TCGGAACTCC 
ACTGAGCTCT CAGAGATGTT TCCTGTCCTC CCCGGTTCCC ACTTGCTGCT CAATAACCCT 
GCCCTGGAGT TCATCAAATA CGTGTGCAAG GTGCTGTCCC TGGACACCAA CATCACAAAC 
CAGGTGAATA AGCTGAACCG AGACCTGCTT CGCCTGGTGG ATGTCGGCGA GTTCTCCGAG 
GAGGCCCAGT TCCGAGACCC CTGCCGCTCC TACGTGCTTC CTGAGGTCAT CTGCCGCAGC 
TGTAACTTCT GCCGCGACCT GGACCTGTGT AAAGACTCTT CCTTCTCAGA GGATGGGGCG 
GTCCTGCCTC AGTGGCTCTG CTCCAACTGT CAGGCGCCCT ACGACTCCTC TGCCATCGAG 
ATGACGCTGG TGGAAGTTCT ACAGAAGAAG CTGATGGCCT TCACCCTGCA GGACCTGGTC 
TGCCTGAAGT GCCGCGGGGT GAAGGAGACC AGCATGCCTG TGTACTGCAG CTGCGCGGGA 
GACTTCGCCC TCACCATCCA CACCCAGGTC TTCATGGAAC AGATCGGAAT ATTCCGGAAC 
ATTGCCCAGC ACTACGGCAT GTCGTACCTC CTGGAGACCC TGGAGTGGCT GCTGCAGAAG 
AACCCACAGC TGGGCCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( NM_006231.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006222.2 Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), mRNA.

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CloneID	OHu20900
Clone ID Related Accession (Same CDS sequence)	NM_006231.3 , NM_006231.4
Accession Version	NM_006231.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Homo sapiens(human)
Product	DNA polymerase epsilon catalytic subunit A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC135586.8, L09561.1, CX782956.1 and BC011376.1. This sequence is a reference standard in the RefSeqGene project. On Sep 26, 2013 this sequence version replaced NM_006231.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L09561.1, U49356.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG 
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT 
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT 
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC 
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC 
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG 
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC 
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC 
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG 
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA 
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA 
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC 
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG 
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC 
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT 
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC 
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG 
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT 
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG 
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA 
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC 
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT 
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC 
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT 
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG 
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC 
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA 
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG 
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC 
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG 
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG 
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG 
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG 
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC 
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT 
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG 
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA 
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG 
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC 
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA 
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA 
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC 
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT 
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC 
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG 
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG 
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC 
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT 
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA 
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC 
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG 
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG 
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT 
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG 
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC 
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA 
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC 
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC 
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG 
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC 
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG 
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT 
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG 
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC 
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA 
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT 
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC 
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC 
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC 
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGA TCCTGAAGAG CATGGTCGTG 
GGCTGGGTGA AGGAGATCAC CCAGTACCAC AACATCTATG CAGACAACCA GGTGATGCAC 
TTCTACCGCT GGCTTCGGTC GCCATCCTCT CTGCTTCATG ACCCTGCCCT GCACCGCACA 
CTCCACAACA TGATGAAGAA GCTCTTCCTG CAGCTCATCG CTGAGTTCAA GCGCCTGGGG 
TCATCAGTCA TCTACGCCAA CTTCAACCGC ATCATCCTCT GTACAAAGAA GCGCCGTGTG 
GAAGATGCCA TCGCTTACGT GGAGTACATC ACCAGCAGCA TCCATTCAAA GGAGACCTTC 
CATTCTCTGA CAATTTCTTT CTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCATCT 
AACTATGGCG GAATCAAAGG AAAAGTTTCA TCTCGTATTC ACTGTGGACT GCAAGACTCC 
CAGAAAGCAG GGGGAGCAGA GGATGAGCAG GAAAATGAGG ACGATGAGGA GGAAAGAGAT 
GGGGAGGAGG AGGAAGAGGC GGAGGAATCC AACGTGGAGG ATTTACTGGA AAACAACTGG 
AACATTTTGC AGTTTTTGCC ACAGGCAGCC TCCTGCCAGA ACTACTTCCT CATGATTGTT 
TCAGCGTACA TCGTGGCCGT GTACCACTGC ATGAAGGACG GGCTGAGGCG CAGTGCTCCA 
GGGAGCACCC CCGTGAGGAG GAGGGGGGCC AGCCAGCTCT CCCAGGAGGC CGAGGGGGCG 
GTCGGAGCCC TTCCCGGAAT GATCACCTTC TCTCAGGATT ATGTCGCAAA TGAGCTCACT 
CAGAGCTTCT TCACCATCAC TCAGAAGATT CAGAAGAAAG TCACAGGCTC TCGGAACTCC 
ACTGAGCTCT CAGAGATGTT TCCTGTCCTC CCCGGTTCCC ACTTGCTGCT CAATAACCCT 
GCCCTGGAGT TCATCAAATA CGTGTGCAAG GTGCTGTCCC TGGACACCAA CATCACAAAC 
CAGGTGAATA AGCTGAACCG AGACCTGCTT CGCCTGGTGG ATGTCGGCGA GTTCTCCGAG 
GAGGCCCAGT TCCGAGACCC CTGCCGCTCC TACGTGCTTC CTGAGGTCAT CTGCCGCAGC 
TGTAACTTCT GCCGCGACCT GGACCTGTGT AAAGACTCTT CCTTCTCAGA GGATGGGGCG 
GTCCTGCCTC AGTGGCTCTG CTCCAACTGT CAGGCGCCCT ACGACTCCTC TGCCATCGAG 
ATGACGCTGG TGGAAGTTCT ACAGAAGAAG CTGATGGCCT TCACCCTGCA GGACCTGGTC 
TGCCTGAAGT GCCGCGGGGT GAAGGAGACC AGCATGCCTG TGTACTGCAG CTGCGCGGGA 
GACTTCGCCC TCACCATCCA CACCCAGGTC TTCATGGAAC AGATCGGAAT ATTCCGGAAC 
ATTGCCCAGC ACTACGGCAT GTCGTACCTC CTGGAGACCC TGGAGTGGCT GCTGCAGAAG 
AACCCACAGC TGGGCCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006222.2
CDS	210..7070
Translation	MSLRSGGRRRADPGADGEASRDDGATSSVSALKRLERSQWTDKMDLRFGFERLKEPGEKTGWLINMHPTEILDEDKRLGSAVDYYFIQDDGSRFKVALPYKPYFYIATRKGCEREVSSFLSKKFQGKIAKVETVPKEDLDLPNHLVGLKRNYIRLSFHTVEDLVKVRKEISPAVKKNREQDHASDAYTALLSSVLQRGGVITDEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNVRYRGNAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEAHLIQRWFEHVQETKPTIMVTYNGDFFDWPFVEARAAVHGLSMQQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDSETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTLRHALEEEEKVPVEQVTNFEEVCDEIKSKLASLKDVPSRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHITKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVFKTTNVKKPKVTISYPGAMLNIMVKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRAILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFTLEGRRQVTMAEASEDSPRPSAPDMEDFGLVKLPHPAAPVTVKRKRVLWESQEESQDLTPTVPWQEILGQPPALGTSQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEGVLRPGAIRDGPATGLGSFLRRTARSILDLPWQIVQISETSQAGLFRLWALVGSDLHCIRLSIPRVFYVNQRVAKAEEGASYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINAELSAPDIEGVYETQVPLLFRALVHLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHAQAHKALFGIFIPSQRRASVFVLDTVRSNQMPSLGALYSAEHGLLLEKVGPELLPPPKHTFEVRAETDLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELKRLASEIPVLEEFPLVPICVADKINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTARPDLGGKEADDNCLVMEFDDQATVEINSSGCYSTVCVELDLQNLAVNTILQSHHVNDMEGADSMGISFDVIQQASLEDMITGGQAASAPASYDETALCSNTFRILKSMVVGWVKEITQYHNIYADNQVMHFYRWLRSPSSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVIYANFNRIILCTKKRRVEDAIAYVEYITSSIHSKETFHSLTISFSRCWEFLLWMDPSNYGGIKGKVSSRIHCGLQDSQKAGGAEDEQENEDDEEERDGEEEEEAEESNVEDLLENNWNILQFLPQAASCQNYFLMIVSAYIVAVYHCMKDGLRRSAPGSTPVRRRGASQLSQEAEGAVGALPGMITFSQDYVANELTQSFFTITQKIQKKVTGSRNSTELSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCKDSSFSEDGAVLPQWLCSNCQAPYDSSAIEMTLVEVLQKKLMAFTLQDLVCLKCRGVKETSMPVYCSCAGDFALTIHTQVFMEQIGIFRNIAQHYGMSYLLETLEWLLQKNPQLGH

Target ORF information:

RefSeq Version	NM_006231.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006231.3

ORF Insert Sequence:

ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG 
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT 
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT 
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC 
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC 
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG 
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC 
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC 
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG 
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA 
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA 
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC 
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG 
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC 
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT 
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC 
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG 
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT 
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG 
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA 
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC 
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT 
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC 
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT 
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG 
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC 
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA 
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG 
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC 
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG 
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG 
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG 
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG 
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC 
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT 
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG 
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA 
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG 
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC 
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA 
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA 
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC 
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT 
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC 
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG 
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG 
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC 
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT 
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA 
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC 
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG 
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG 
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT 
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG 
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC 
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA 
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC 
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC 
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG 
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC 
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG 
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT 
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG 
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC 
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA 
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT 
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC 
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC 
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC 
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGA TCCTGAAGAG CATGGTCGTG 
GGCTGGGTGA AGGAGATCAC CCAGTACCAC AACATCTATG CAGACAACCA GGTGATGCAC 
TTCTACCGCT GGCTTCGGTC GCCATCCTCT CTGCTTCATG ACCCTGCCCT GCACCGCACA 
CTCCACAACA TGATGAAGAA GCTCTTCCTG CAGCTCATCG CTGAGTTCAA GCGCCTGGGG 
TCATCAGTCA TCTACGCCAA CTTCAACCGC ATCATCCTCT GTACAAAGAA GCGCCGTGTG 
GAAGATGCCA TCGCTTACGT GGAGTACATC ACCAGCAGCA TCCATTCAAA GGAGACCTTC 
CATTCTCTGA CAATTTCTTT CTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCATCT 
AACTATGGCG GAATCAAAGG AAAAGTTTCA TCTCGTATTC ACTGTGGACT GCAAGACTCC 
CAGAAAGCAG GGGGAGCAGA GGATGAGCAG GAAAATGAGG ACGATGAGGA GGAAAGAGAT 
GGGGAGGAGG AGGAAGAGGC GGAGGAATCC AACGTGGAGG ATTTACTGGA AAACAACTGG 
AACATTTTGC AGTTTTTGCC ACAGGCAGCC TCCTGCCAGA ACTACTTCCT CATGATTGTT 
TCAGCGTACA TCGTGGCCGT GTACCACTGC ATGAAGGACG GGCTGAGGCG CAGTGCTCCA 
GGGAGCACCC CCGTGAGGAG GAGGGGGGCC AGCCAGCTCT CCCAGGAGGC CGAGGGGGCG 
GTCGGAGCCC TTCCCGGAAT GATCACCTTC TCTCAGGATT ATGTCGCAAA TGAGCTCACT 
CAGAGCTTCT TCACCATCAC TCAGAAGATT CAGAAGAAAG TCACAGGCTC TCGGAACTCC 
ACTGAGCTCT CAGAGATGTT TCCTGTCCTC CCCGGTTCCC ACTTGCTGCT CAATAACCCT 
GCCCTGGAGT TCATCAAATA CGTGTGCAAG GTGCTGTCCC TGGACACCAA CATCACAAAC 
CAGGTGAATA AGCTGAACCG AGACCTGCTT CGCCTGGTGG ATGTCGGCGA GTTCTCCGAG 
GAGGCCCAGT TCCGAGACCC CTGCCGCTCC TACGTGCTTC CTGAGGTCAT CTGCCGCAGC 
TGTAACTTCT GCCGCGACCT GGACCTGTGT AAAGACTCTT CCTTCTCAGA GGATGGGGCG 
GTCCTGCCTC AGTGGCTCTG CTCCAACTGT CAGGCGCCCT ACGACTCCTC TGCCATCGAG 
ATGACGCTGG TGGAAGTTCT ACAGAAGAAG CTGATGGCCT TCACCCTGCA GGACCTGGTC 
TGCCTGAAGT GCCGCGGGGT GAAGGAGACC AGCATGCCTG TGTACTGCAG CTGCGCGGGA 
GACTTCGCCC TCACCATCCA CACCCAGGTC TTCATGGAAC AGATCGGAAT ATTCCGGAAC 
ATTGCCCAGC ACTACGGCAT GTCGTACCTC CTGGAGACCC TGGAGTGGCT GCTGCAGAAG 
AACCCACAGC TGGGCCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_011534795.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533097.1 Homo sapiens polymerase (DNA) epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.

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CloneID	OHu59808
Clone ID Related Accession (Same CDS sequence)	XM_011534795.2 , XM_011534795.3
Accession Version	XM_011534795.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DNA polymerase epsilon catalytic subunit A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534795.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG 
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT 
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT 
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC 
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC 
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG 
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC 
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC 
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG 
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA 
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA 
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC 
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG 
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC 
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT 
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC 
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG 
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT 
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG 
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA 
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC 
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT 
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC 
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT 
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG 
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC 
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA 
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG 
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC 
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG 
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG 
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG 
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG 
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC 
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT 
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG 
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA 
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG 
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC 
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA 
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA 
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC 
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT 
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC 
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG 
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG 
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC 
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT 
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA 
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC 
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG 
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG 
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT 
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG 
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC 
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA 
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC 
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC 
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG 
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC 
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG 
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT 
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG 
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC 
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA 
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT 
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC 
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC 
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC 
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGA TCCTGAAGAG CATGGTCGTG 
GGCTGGGTGA AGGAGATCAC CCAGTACCAC AACATCTATG CAGACAACCA GGTGATGCAC 
TTCTACCGCT GGCTTCGGTC GCCATCCTCT CTGCTTCATG ACCCTGCCCT GCACCGCACA 
CTCCACAACA TGATGAAGAA GCTCTTCCTG CAGCTCATCG CTGAGTTCAA GCGCCTGGGG 
TCATCAGTCA TCTACGCCAA CTTCAACCGC ATCATCCTCT GTACAAAGAA GCGCCGTGTG 
GAAGATGCCA TCGCTTACGT GGAGTACATC ACCAGCAGCA TCCATTCAAA GGAGACCTTC 
CATTCTCTGA CAATTTCTTT CTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCATCT 
AACTATGGCG GAATCAAAGG AAAAGTTTCA TCTCGTATTC ACTGTGGACT GCAAGACTCC 
CAGAAAGCAG GGGGAGCAGA GGATGAGCAG GAAAATGAGG ACGATGAGGA GGAAAGAGAT 
GGGGAGGAGG AGGAAGAGGC GGAGGAATCC AACGTGGAGG ATTTACTGGA AAACAACTGG 
AACATTTTGC AGTTTTTGCC ACAGGCAGCC TCCTGCCAGA ACTACTTCCT CATGATTGTT 
TCAGCGTACA TCGTGGCCGT GTACCACTGC ATGAAGGACG GGCTGAGGCG CAGTGCTCCA 
GGGAGCACCC CCGTGAGGAG GAGGGGGGCC AGCCAGCTCT CCCAGGAGGC CGAGGGGGCG 
GTCGGAGCCC TTCCCGGAAT GATCACCTTC TCTCAGGATT ATGTCGCAAA TGAGCTCACT 
CAGAGCTTCT TCACCATCAC TCAGAAGATT CAGAAGAAAG TCACAGGCTC TCGGAACTCC 
ACTGAGCTCT CAGAGATGTT TCCTGTCCTC CCCGGTTCCC ACTTGCTGCT CAATAACCCT 
GCCCTGGAGT TCATCAAATA CGTGTGCAAG GTGCTGTCCC TGGACACCAA CATCACAAAC 
CAGGTGAATA AGCTGAACCG AGACCTGCTT CGCCTGGTGG ATGTCGGCGA GTTCTCCGAG 
GAGGCCCAGT TCCGAGACCC CTGCCGCTCC TACGTGCTTC CTGAGGTCAT CTGCCGCAGC 
TGTAACTTCT GCCGCGACCT GGACCTGTGT AAAGACTCTT CCTTCTCAGA GGATGGGGCG 
GTCCTGCCTC AGTGGCTCTG CTCCAACTGT CAGGCGCCCT ACGACTCCTC TGCCATCGAG 
ATGACGCTGG TGGAAGTTCT ACAGAAGAAG CTGATGGCCT TCACCCTGCA GGACCTGCGG 
AAGGAGGCAG ACACTCCCAA GTGTGAAGAG AAGGAAAGGA GGAAGAGGGA GTTGCACAGC 
TCGGCCACAG GAAGGTCTTG CCGAGGGGCA TCTGAGCAGA GGCTGGGGGA CGCGCCAAGC 
TGCCGTGTGG TGGTGCACGG CGTTCAGGCC TGCAGGATGG AGGGAGTGAG CAGGGCCTGG 
AGGAGGCCTC AGGACTGGGC ATCTGGGGTG GAACATCCAC AGCGAGGGTG GGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533097.1
CDS	205..7101
Translation	MSLRSGGRRRADPGADGEASRDDGATSSVSALKRLERSQWTDKMDLRFGFERLKEPGEKTGWLINMHPTEILDEDKRLGSAVDYYFIQDDGSRFKVALPYKPYFYIATRKGCEREVSSFLSKKFQGKIAKVETVPKEDLDLPNHLVGLKRNYIRLSFHTVEDLVKVRKEISPAVKKNREQDHASDAYTALLSSVLQRGGVITDEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNVRYRGNAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEAHLIQRWFEHVQETKPTIMVTYNGDFFDWPFVEARAAVHGLSMQQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDSETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTLRHALEEEEKVPVEQVTNFEEVCDEIKSKLASLKDVPSRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHITKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVFKTTNVKKPKVTISYPGAMLNIMVKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRAILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFTLEGRRQVTMAEASEDSPRPSAPDMEDFGLVKLPHPAAPVTVKRKRVLWESQEESQDLTPTVPWQEILGQPPALGTSQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEGVLRPGAIRDGPATGLGSFLRRTARSILDLPWQIVQISETSQAGLFRLWALVGSDLHCIRLSIPRVFYVNQRVAKAEEGASYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINAELSAPDIEGVYETQVPLLFRALVHLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHAQAHKALFGIFIPSQRRASVFVLDTVRSNQMPSLGALYSAEHGLLLEKVGPELLPPPKHTFEVRAETDLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELKRLASEIPVLEEFPLVPICVADKINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTARPDLGGKEADDNCLVMEFDDQATVEINSSGCYSTVCVELDLQNLAVNTILQSHHVNDMEGADSMGISFDVIQQASLEDMITGGQAASAPASYDETALCSNTFRILKSMVVGWVKEITQYHNIYADNQVMHFYRWLRSPSSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVIYANFNRIILCTKKRRVEDAIAYVEYITSSIHSKETFHSLTISFSRCWEFLLWMDPSNYGGIKGKVSSRIHCGLQDSQKAGGAEDEQENEDDEEERDGEEEEEAEESNVEDLLENNWNILQFLPQAASCQNYFLMIVSAYIVAVYHCMKDGLRRSAPGSTPVRRRGASQLSQEAEGAVGALPGMITFSQDYVANELTQSFFTITQKIQKKVTGSRNSTELSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCKDSSFSEDGAVLPQWLCSNCQAPYDSSAIEMTLVEVLQKKLMAFTLQDLRKEADTPKCEEKERRKRELHSSATGRSCRGASEQRLGDAPSCRVVVHGVQACRMEGVSRAWRRPQDWASGVEHPQRGWV

Target ORF information:

RefSeq Version	XM_011534795.2
Organism	Homo sapiens(human)
Definition	Homo sapiens polymerase (DNA) epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534795.2

ORF Insert Sequence:

ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG 
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT 
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT 
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC 
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC 
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG 
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC 
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC 
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG 
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA 
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA 
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC 
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG 
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC 
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT 
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC 
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG 
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT 
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG 
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA 
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC 
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT 
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC 
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT 
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG 
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC 
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA 
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG 
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC 
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG 
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG 
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG 
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG 
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC 
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT 
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG 
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA 
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG 
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC 
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA 
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA 
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC 
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT 
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC 
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG 
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG 
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC 
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT 
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA 
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC 
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG 
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG 
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT 
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG 
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC 
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA 
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC 
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC 
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG 
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC 
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG 
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT 
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG 
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC 
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA 
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT 
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC 
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC 
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC 
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGA TCCTGAAGAG CATGGTCGTG 
GGCTGGGTGA AGGAGATCAC CCAGTACCAC AACATCTATG CAGACAACCA GGTGATGCAC 
TTCTACCGCT GGCTTCGGTC GCCATCCTCT CTGCTTCATG ACCCTGCCCT GCACCGCACA 
CTCCACAACA TGATGAAGAA GCTCTTCCTG CAGCTCATCG CTGAGTTCAA GCGCCTGGGG 
TCATCAGTCA TCTACGCCAA CTTCAACCGC ATCATCCTCT GTACAAAGAA GCGCCGTGTG 
GAAGATGCCA TCGCTTACGT GGAGTACATC ACCAGCAGCA TCCATTCAAA GGAGACCTTC 
CATTCTCTGA CAATTTCTTT CTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCATCT 
AACTATGGCG GAATCAAAGG AAAAGTTTCA TCTCGTATTC ACTGTGGACT GCAAGACTCC 
CAGAAAGCAG GGGGAGCAGA GGATGAGCAG GAAAATGAGG ACGATGAGGA GGAAAGAGAT 
GGGGAGGAGG AGGAAGAGGC GGAGGAATCC AACGTGGAGG ATTTACTGGA AAACAACTGG 
AACATTTTGC AGTTTTTGCC ACAGGCAGCC TCCTGCCAGA ACTACTTCCT CATGATTGTT 
TCAGCGTACA TCGTGGCCGT GTACCACTGC ATGAAGGACG GGCTGAGGCG CAGTGCTCCA 
GGGAGCACCC CCGTGAGGAG GAGGGGGGCC AGCCAGCTCT CCCAGGAGGC CGAGGGGGCG 
GTCGGAGCCC TTCCCGGAAT GATCACCTTC TCTCAGGATT ATGTCGCAAA TGAGCTCACT 
CAGAGCTTCT TCACCATCAC TCAGAAGATT CAGAAGAAAG TCACAGGCTC TCGGAACTCC 
ACTGAGCTCT CAGAGATGTT TCCTGTCCTC CCCGGTTCCC ACTTGCTGCT CAATAACCCT 
GCCCTGGAGT TCATCAAATA CGTGTGCAAG GTGCTGTCCC TGGACACCAA CATCACAAAC 
CAGGTGAATA AGCTGAACCG AGACCTGCTT CGCCTGGTGG ATGTCGGCGA GTTCTCCGAG 
GAGGCCCAGT TCCGAGACCC CTGCCGCTCC TACGTGCTTC CTGAGGTCAT CTGCCGCAGC 
TGTAACTTCT GCCGCGACCT GGACCTGTGT AAAGACTCTT CCTTCTCAGA GGATGGGGCG 
GTCCTGCCTC AGTGGCTCTG CTCCAACTGT CAGGCGCCCT ACGACTCCTC TGCCATCGAG 
ATGACGCTGG TGGAAGTTCT ACAGAAGAAG CTGATGGCCT TCACCCTGCA GGACCTGCGG 
AAGGAGGCAG ACACTCCCAA GTGTGAAGAG AAGGAAAGGA GGAAGAGGGA GTTGCACAGC 
TCGGCCACAG GAAGGTCTTG CCGAGGGGCA TCTGAGCAGA GGCTGGGGGA CGCGCCAAGC 
TGCCGTGTGG TGGTGCACGG CGTTCAGGCC TGCAGGATGG AGGGAGTGAG CAGGGCCTGG 
AGGAGGCCTC AGGACTGGGC ATCTGGGGTG GAACATCCAC AGCGAGGGTG GGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLE ( XM_011534795.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533097.1 Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.

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CloneID	OHu59808
Clone ID Related Accession (Same CDS sequence)	XM_011534795.2 , XM_011534795.3
Accession Version	XM_011534795.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA polymerase epsilon catalytic subunit A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011534795.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG 
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT 
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT 
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC 
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC 
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG 
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC 
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC 
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG 
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA 
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA 
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC 
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG 
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC 
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT 
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC 
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG 
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT 
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG 
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA 
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC 
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT 
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC 
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT 
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG 
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC 
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA 
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG 
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC 
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG 
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG 
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG 
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG 
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC 
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT 
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG 
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA 
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG 
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC 
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA 
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA 
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC 
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT 
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC 
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG 
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG 
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC 
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT 
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA 
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC 
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG 
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG 
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT 
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG 
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC 
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA 
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC 
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC 
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG 
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC 
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG 
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT 
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG 
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC 
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA 
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT 
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC 
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC 
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC 
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGA TCCTGAAGAG CATGGTCGTG 
GGCTGGGTGA AGGAGATCAC CCAGTACCAC AACATCTATG CAGACAACCA GGTGATGCAC 
TTCTACCGCT GGCTTCGGTC GCCATCCTCT CTGCTTCATG ACCCTGCCCT GCACCGCACA 
CTCCACAACA TGATGAAGAA GCTCTTCCTG CAGCTCATCG CTGAGTTCAA GCGCCTGGGG 
TCATCAGTCA TCTACGCCAA CTTCAACCGC ATCATCCTCT GTACAAAGAA GCGCCGTGTG 
GAAGATGCCA TCGCTTACGT GGAGTACATC ACCAGCAGCA TCCATTCAAA GGAGACCTTC 
CATTCTCTGA CAATTTCTTT CTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCATCT 
AACTATGGCG GAATCAAAGG AAAAGTTTCA TCTCGTATTC ACTGTGGACT GCAAGACTCC 
CAGAAAGCAG GGGGAGCAGA GGATGAGCAG GAAAATGAGG ACGATGAGGA GGAAAGAGAT 
GGGGAGGAGG AGGAAGAGGC GGAGGAATCC AACGTGGAGG ATTTACTGGA AAACAACTGG 
AACATTTTGC AGTTTTTGCC ACAGGCAGCC TCCTGCCAGA ACTACTTCCT CATGATTGTT 
TCAGCGTACA TCGTGGCCGT GTACCACTGC ATGAAGGACG GGCTGAGGCG CAGTGCTCCA 
GGGAGCACCC CCGTGAGGAG GAGGGGGGCC AGCCAGCTCT CCCAGGAGGC CGAGGGGGCG 
GTCGGAGCCC TTCCCGGAAT GATCACCTTC TCTCAGGATT ATGTCGCAAA TGAGCTCACT 
CAGAGCTTCT TCACCATCAC TCAGAAGATT CAGAAGAAAG TCACAGGCTC TCGGAACTCC 
ACTGAGCTCT CAGAGATGTT TCCTGTCCTC CCCGGTTCCC ACTTGCTGCT CAATAACCCT 
GCCCTGGAGT TCATCAAATA CGTGTGCAAG GTGCTGTCCC TGGACACCAA CATCACAAAC 
CAGGTGAATA AGCTGAACCG AGACCTGCTT CGCCTGGTGG ATGTCGGCGA GTTCTCCGAG 
GAGGCCCAGT TCCGAGACCC CTGCCGCTCC TACGTGCTTC CTGAGGTCAT CTGCCGCAGC 
TGTAACTTCT GCCGCGACCT GGACCTGTGT AAAGACTCTT CCTTCTCAGA GGATGGGGCG 
GTCCTGCCTC AGTGGCTCTG CTCCAACTGT CAGGCGCCCT ACGACTCCTC TGCCATCGAG 
ATGACGCTGG TGGAAGTTCT ACAGAAGAAG CTGATGGCCT TCACCCTGCA GGACCTGCGG 
AAGGAGGCAG ACACTCCCAA GTGTGAAGAG AAGGAAAGGA GGAAGAGGGA GTTGCACAGC 
TCGGCCACAG GAAGGTCTTG CCGAGGGGCA TCTGAGCAGA GGCTGGGGGA CGCGCCAAGC 
TGCCGTGTGG TGGTGCACGG CGTTCAGGCC TGCAGGATGG AGGGAGTGAG CAGGGCCTGG 
AGGAGGCCTC AGGACTGGGC ATCTGGGGTG GAACATCCAC AGCGAGGGTG GGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533097.1
CDS	205..7101
Translation	MSLRSGGRRRADPGADGEASRDDGATSSVSALKRLERSQWTDKMDLRFGFERLKEPGEKTGWLINMHPTEILDEDKRLGSAVDYYFIQDDGSRFKVALPYKPYFYIATRKGCEREVSSFLSKKFQGKIAKVETVPKEDLDLPNHLVGLKRNYIRLSFHTVEDLVKVRKEISPAVKKNREQDHASDAYTALLSSVLQRGGVITDEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNVRYRGNAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEAHLIQRWFEHVQETKPTIMVTYNGDFFDWPFVEARAAVHGLSMQQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDSETYVGGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTLRHALEEEEKVPVEQVTNFEEVCDEIKSKLASLKDVPSRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHITKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVFKTTNVKKPKVTISYPGAMLNIMVKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRAILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFTLEGRRQVTMAEASEDSPRPSAPDMEDFGLVKLPHPAAPVTVKRKRVLWESQEESQDLTPTVPWQEILGQPPALGTSQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEGVLRPGAIRDGPATGLGSFLRRTARSILDLPWQIVQISETSQAGLFRLWALVGSDLHCIRLSIPRVFYVNQRVAKAEEGASYRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINAELSAPDIEGVYETQVPLLFRALVHLGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHAQAHKALFGIFIPSQRRASVFVLDTVRSNQMPSLGALYSAEHGLLLEKVGPELLPPPKHTFEVRAETDLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELKRLASEIPVLEEFPLVPICVADKINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTARPDLGGKEADDNCLVMEFDDQATVEINSSGCYSTVCVELDLQNLAVNTILQSHHVNDMEGADSMGISFDVIQQASLEDMITGGQAASAPASYDETALCSNTFRILKSMVVGWVKEITQYHNIYADNQVMHFYRWLRSPSSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVIYANFNRIILCTKKRRVEDAIAYVEYITSSIHSKETFHSLTISFSRCWEFLLWMDPSNYGGIKGKVSSRIHCGLQDSQKAGGAEDEQENEDDEEERDGEEEEEAEESNVEDLLENNWNILQFLPQAASCQNYFLMIVSAYIVAVYHCMKDGLRRSAPGSTPVRRRGASQLSQEAEGAVGALPGMITFSQDYVANELTQSFFTITQKIQKKVTGSRNSTELSEMFPVLPGSHLLLNNPALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRSCNFCRDLDLCKDSSFSEDGAVLPQWLCSNCQAPYDSSAIEMTLVEVLQKKLMAFTLQDLRKEADTPKCEEKERRKRELHSSATGRSCRGASEQRLGDAPSCRVVVHGVQACRMEGVSRAWRRPQDWASGVEHPQRGWV

Target ORF information:

RefSeq Version	XM_011534795.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534795.3

ORF Insert Sequence:

ATGTCTCTGA GGAGCGGCGG GCGGCGGCGC GCGGACCCAG GCGCGGATGG CGAGGCCAGC 
AGGGATGATG GCGCCACTTC CTCAGTTTCG GCACTCAAGC GCCTGGAACG GAGTCAGTGG 
ACGGATAAGA TGGATTTGCG GTTTGGTTTT GAGCGGCTGA AGGAGCCTGG TGAGAAGACA 
GGCTGGCTCA TTAACATGCA TCCTACCGAG ATTTTAGATG AAGATAAGCG CTTAGGCAGT 
GCAGTGGATT ACTACTTTAT TCAAGATGAC GGAAGCAGAT TTAAGGTGGC TTTGCCCTAT 
AAACCGTATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTC 
TCCAAGAAGT TTCAGGGCAA AATTGCAAAA GTGGAGACTG TCCCCAAAGA GGATCTGGAC 
TTGCCAAATC ACTTGGTGGG TTTGAAGCGA AATTACATCA GGCTGTCCTT CCACACTGTG 
GAGGATCTTG TCAAAGTGAG GAAGGAGATC TCCCCTGCCG TGAAGAAGAA CAGGGAGCAG 
GATCACGCCA GCGACGCGTA CACAGCTCTG CTTTCCAGTG TTCTGCAGAG GGGCGGTGTC 
ATTACTGATG AAGAGGAAAC CTCTAAGAAG ATAGCTGACC AGTTGGACAA CATTGTGGAC 
ATGCGCGAGT ACGATGTTCC CTACCACATC CGCCTCTCCA TTGACCTGAA GATCCACGTG 
GCTCATTGGT ACAATGTCAG ATACCGAGGA AATGCTTTTC CGGTAGAAAT CACCCGCCGA 
GATGACCTTG TTGAACGACC TGACCCTGTG GTTTTGGCAT TTGACATTGA GACGACCAAA 
CTGCCCCTCA AGTTTCCTGA TGCTGAGACA GACCAGATTA TGATGATTTC CTACATGATC 
GATGGCCAGG GCTACCTCAT CACCAACAGG GAGATTGTTT CAGAAGATAT TGAAGATTTT 
GAGTTCACCC CCAAGCCAGA ATATGAAGGC CCCTTTTGTG TCTTCAATGA ACCCGATGAG 
GCTCATCTGA TCCAAAGGTG GTTTGAACAC GTCCAGGAGA CCAAACCCAC CATCATGGTC 
ACCTACAACG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCCGGGCAGC AGTCCACGGT 
CTGAGCATGC AGCAGGAGAT AGGCTTCCAG AAGGACAGCC AGGGGGAGTA CAAGGCGCCC 
CAGTGCATCC ACATGGACTG CCTCAGGTGG GTGAAGAGGG ACAGTTACCT TCCTGTGGGC 
AGTCATAATC TCAAGGCGGC CGCCAAGGCC AAGCTAGGCT ATGATCCCGT GGAGCTAGAC 
CCGGAGGACA TGTGCCGGAT GGCCACGGAG CAGCCCCAGA CTCTGGCCAC GTATTCTGTG 
TCAGATGCTG TCGCCACTTA CTACCTGTAC ATGAAGTACG TCCACCCATT CATCTTTGCT 
CTGTGCACCA TTATTCCCAT GGAGCCCGAC GAGGTGCTGC GGAAGGGCTC TGGCACTCTG 
TGTGAGGCCT TGCTGATGGT GCAGGCCTTC CACGCCAACA TCATCTTCCC CAACAAGCAA 
GAGCAGGAGT TCAATAAGCT GACGGACGAC GGACACGTGC TGGACTCTGA GACCTACGTC 
GGGGGCCACG TGGAGGCCCT CGAGTCTGGG GTTTTCCGCA GCGATATCCC TTGCCGGTTT 
AGGATGAATC CTGCCGCCTT TGACTTCCTG CTGCAGCGGG TTGAGAAGAC CTTGCGCCAC 
GCCCTTGAGG AAGAGGAGAA AGTGCCTGTG GAGCAAGTCA CCAACTTTGA AGAGGTGTGT 
GATGAGATTA AGAGCAAGCT TGCCTCCCTG AAGGACGTTC CCAGCCGCAT CGAGTGTCCA 
CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCCTGAC CAACCGCCTG 
CAGCCCTCTG CCATGGTGGA CGAAGCCACC TGTGCTGCCT GTGACTTCAA TAAGCCTGGA 
GCAAACTGCC AGCGGAAGAT GGCCTGGCAG TGGAGGGGCG AGTTCATGCC AGCCAGTCGC 
AGCGAATACC ATCGGATCCA GCACCAGCTG GAGTCAGAGA AGTTCCCCCC CTTGTTCCCA 
GAGGGGCCAG CTCGGGCCTT TCATGAACTG TCCCGCGAGG AACAGGCGAA ATACGAGAAG 
AGAAGGCTGG CGGATTACTG CCGGAAAGCC TACAAGAAGA TCCACATCAC CAAGGTGGAA 
GAGCGTCTCA CCACCATCTG CCAGCGGGAA AACTCCTTCT ACGTGGACAC CGTGCGTGCC 
TTCCGGGACA GGCGTTACGA GTTCAAAGGG CTCCACAAGG TGTGGAAAAA GAAGCTCTCG 
GCGGCCGTGG AGGTGGGCGA CGCGGCTGAG GTGAAGCGCT GCAAGAACAT GGAGGTGCTG 
TATGACTCGC TGCAGCTGGC CCACAAGTGC ATCCTGAACT CCTTCTATGG CTATGTCATG 
CGCAAGGGGG CTCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC 
AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAGATTG GGAGGCCCTT AGAGCTGGAC 
ACAGATGGTA TATGGTGCGT CCTGCCCAAC AGCTTCCCAG AAAATTTTGT CTTCAAGACG 
ACCAATGTGA AGAAGCCCAA AGTGACCATC TCCTACCCAG GCGCCATGTT GAACATCATG 
GTCAAGGAAG GCTTCACCAA TGACCAGTAC CAGGAGCTGG CTGAGCCGTC CTCACTCACC 
TACGTCACCC GCTCAGAGAA CAGCATCTTT TTTGAGGTTG ATGGGCCCTA CCTTGCCATG 
ATTCTTCCAG CCTCCAAGGA AGAAGGCAAG AAATTGAAGA AGAGGTATGC TGTGTTCAAT 
GAAGACGGTT CTCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGCGG GGAACTGCAG 
CTGATTAAGA TCTTCCAATC CTCGGTGTTT GAGGCCTTCC TCAAGGGCAG CACGCTGGAA 
GAGGTGTATG GCTCTGTAGC CAAGGTGGCT GACTACTGGC TGGACGTGCT GTACAGCAAG 
GCAGCCAACA TGCCTGACTC TGAGCTATTC GAGCTCATCT CTGAGAACCG TTCCATGTCT 
CGGAAGCTGG AAGATTACGG GGAGCAGAAG TCTACGTCCA TCAGCACAGC AAAGCGCCTG 
GCCGAGTTCC TGGGAGACCA GATGGTCAAG GATGCAGGGC TGAGTTGCCG CTACATCATC 
TCCCGCAAGC CCGAGGGCTC CCCTGTCACG GAGAGGGCCA TCCCACTTGC CATTTTCCAA 
GCAGAGCCCA CGGTGAGGAA GCACTTTCTC CGGAAATGGC TCAAGAGCTC TTCCCTTCAA 
GACTTTGATA TTCGAGCAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGAAGCGCC 
ATCCAGAAGA TCATCACCAT CCCTGCGGCC CTGCAGCAGG TAAAGAACCC AGTGCCACGT 
GTCAAACACC CCGACTGGCT GCACAAAAAA CTGCTGGAGA AGAATGATGT CTACAAGCAG 
AAGAAGATCA GTGAGCTCTT CACCCTGGAG GGCAGGAGAC AGGTCACGAT GGCCGAGGCC 
TCAGAAGACA GTCCGAGGCC AAGTGCTCCT GACATGGAGG ACTTCGGCCT CGTAAAGCTG 
CCTCACCCAG CAGCCCCTGT CACTGTGAAG AGGAAGCGAG TTCTTTGGGA GAGCCAGGAG 
GAGTCCCAGG ACCTCACGCC GACTGTGCCC TGGCAGGAAA TCTTGGGGCA GCCTCCCGCC 
CTGGGAACCA GCCAGGAGGA ATGGCTTGTC TGGCTCCGGT TCCACAAGAA GAAGTGGCAG 
CTGCAGGCCC GGCAGCGCCT CGCCCGCAGG AAGAGGCAGC GTCTGGAGTC GGCAGAGGGT 
GTGCTCAGGC CCGGGGCCAT CCGGGATGGT CCTGCCACGG GGCTGGGGAG CTTCTTGCGA 
AGAACTGCCC GCAGCATCCT GGACCTTCCG TGGCAGATTG TGCAGATCAG CGAGACCAGC 
CAGGCCGGCC TGTTCAGGCT GTGGGCGCTC GTTGGCAGTG ACTTGCACTG CATCAGGCTG 
AGCATCCCCC GTGTGTTCTA CGTGAACCAG CGAGTCGCTA AAGCGGAGGA GGGTGCTTCG 
TATCGCAAGG TAAATCGGGT CCTTCCTCGC TCCAACATGG TCTACAATCT CTATGAGTAT 
TCAGTGCCAG AGGACATGTA CCAGGAACAC ATCAACGAGA TCAACGCTGA GCTGTCAGCG 
CCAGACATCG AGGGCGTATA TGAGACTCAG GTTCCGTTAC TGTTCCGGGC CCTGGTGCAC 
CTGGGCTGTG TGTGTGTGGT CAATAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA 
GAGACCTTTG CTCTTGAGCA CCTGGAGATG CGCTCTCTGG CCCAGTTCAG CTACCTGGAA 
CCAGGGAGTA TCCGCCATAT CTACCTGTAC CACCACGCAC AGGCCCACAA AGCGCTCTTC 
GGGATCTTCA TCCCCTCACA GCGCAGGGCA TCCGTCTTTG TGCTGGACAC TGTGCGCAGC 
AACCAGATGC CCAGCCTTGG CGCCCTGTAC TCAGCAGAGC ACGGCCTCCT CCTGGAGAAG 
GTGGGCCCTG AGCTCCTGCC ACCCCCCAAA CACACCTTCG AAGTTCGGGC AGAAACTGAC 
CTGAAGACCA TCTGCAGAGC CATCCAGCGA TTCCTGCTCG CCTACAAGGA GGAGCGCCGG 
GGGCCCACAC TCATCGCTGT TCAGTCCAGC TGGGAGCTGA AGAGGCTGGC CAGTGAAATT 
CCTGTCTTGG AGGAATTCCC ACTGGTGCCT ATCTGTGTGG CTGACAAGAT CAACTATGGG 
GTCCTGGACT GGCAGCGCCA TGGAGCCCGG CGCATGATCC GTCACTACCT CAACCTGGAC 
ACCTGCCTGT CGCAGGCCTT CGAGATGAGC AGGTACTTTC ACATTCCCAT TGGGAACCTA 
CCAGAGGACA TCTCCACATT CGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC 
AACCACCTGC TCTGGCTGTC CCCTACAGCC CGCCCTGACC TGGGTGGAAA GGAGGCTGAT 
GACAACTGTC TTGTCATGGA GTTCGATGAC CAAGCCACTG TTGAGATCAA CAGTTCAGGC 
TGTTACTCCA CAGTGTGTGT GGAGCTGGAC CTTCAGAACC TGGCCGTCAA CACCATTCTC 
CAGTCTCACC ATGTCAACGA CATGGAGGGG GCCGACAGCA TGGGGATCAG CTTCGACGTG 
ATCCAGCAGG CCTCCCTGGA GGACATGATC ACGGGTGGTC AGGCTGCCAG TGCCCCGGCC 
AGCTACGATG AGACAGCCCT GTGCTCTAAC ACCTTCAGGA TCCTGAAGAG CATGGTCGTG 
GGCTGGGTGA AGGAGATCAC CCAGTACCAC AACATCTATG CAGACAACCA GGTGATGCAC 
TTCTACCGCT GGCTTCGGTC GCCATCCTCT CTGCTTCATG ACCCTGCCCT GCACCGCACA 
CTCCACAACA TGATGAAGAA GCTCTTCCTG CAGCTCATCG CTGAGTTCAA GCGCCTGGGG 
TCATCAGTCA TCTACGCCAA CTTCAACCGC ATCATCCTCT GTACAAAGAA GCGCCGTGTG 
GAAGATGCCA TCGCTTACGT GGAGTACATC ACCAGCAGCA TCCATTCAAA GGAGACCTTC 
CATTCTCTGA CAATTTCTTT CTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCATCT 
AACTATGGCG GAATCAAAGG AAAAGTTTCA TCTCGTATTC ACTGTGGACT GCAAGACTCC 
CAGAAAGCAG GGGGAGCAGA GGATGAGCAG GAAAATGAGG ACGATGAGGA GGAAAGAGAT 
GGGGAGGAGG AGGAAGAGGC GGAGGAATCC AACGTGGAGG ATTTACTGGA AAACAACTGG 
AACATTTTGC AGTTTTTGCC ACAGGCAGCC TCCTGCCAGA ACTACTTCCT CATGATTGTT 
TCAGCGTACA TCGTGGCCGT GTACCACTGC ATGAAGGACG GGCTGAGGCG CAGTGCTCCA 
GGGAGCACCC CCGTGAGGAG GAGGGGGGCC AGCCAGCTCT CCCAGGAGGC CGAGGGGGCG 
GTCGGAGCCC TTCCCGGAAT GATCACCTTC TCTCAGGATT ATGTCGCAAA TGAGCTCACT 
CAGAGCTTCT TCACCATCAC TCAGAAGATT CAGAAGAAAG TCACAGGCTC TCGGAACTCC 
ACTGAGCTCT CAGAGATGTT TCCTGTCCTC CCCGGTTCCC ACTTGCTGCT CAATAACCCT 
GCCCTGGAGT TCATCAAATA CGTGTGCAAG GTGCTGTCCC TGGACACCAA CATCACAAAC 
CAGGTGAATA AGCTGAACCG AGACCTGCTT CGCCTGGTGG ATGTCGGCGA GTTCTCCGAG 
GAGGCCCAGT TCCGAGACCC CTGCCGCTCC TACGTGCTTC CTGAGGTCAT CTGCCGCAGC 
TGTAACTTCT GCCGCGACCT GGACCTGTGT AAAGACTCTT CCTTCTCAGA GGATGGGGCG 
GTCCTGCCTC AGTGGCTCTG CTCCAACTGT CAGGCGCCCT ACGACTCCTC TGCCATCGAG 
ATGACGCTGG TGGAAGTTCT ACAGAAGAAG CTGATGGCCT TCACCCTGCA GGACCTGCGG 
AAGGAGGCAG ACACTCCCAA GTGTGAAGAG AAGGAAAGGA GGAAGAGGGA GTTGCACAGC 
TCGGCCACAG GAAGGTCTTG CCGAGGGGCA TCTGAGCAGA GGCTGGGGGA CGCGCCAAGC 
TGCCGTGTGG TGGTGCACGG CGTTCAGGCC TGCAGGATGG AGGGAGTGAG CAGGGCCTGG 
AGGAGGCCTC AGGACTGGGC ATCTGGGGTG GAACATCCAC AGCGAGGGTG GGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	POLE
Entrez Gene ID	5426
Full Name	DNA polymerase epsilon, catalytic subunit
Synonyms	CRCS12,FILS,POLE1
General protein information	Preferred Names DNA polymerase epsilon, catalytic subunit Names DNA polymerase epsilon catalytic subunit A DNA polymerase II subunit A DNA polymerase epsilon catalytic subunit protein polymerase (DNA directed), epsilon, catalytic subunit polymerase (DNA) epsilon, catalytic subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.33
Summary	This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013].
Disorder MIM:	174762
Disorder Html:	{Colorectal cancer, susceptibility to, 12}, 615083 (3); FILS syndrome, 615139 (3)

Danio rerio (zebrafish)	pole	NP_001121995.1
Arabidopsis thaliana (thale cress)	TIL2	NP_180280.2
Arabidopsis thaliana (thale cress)	TIL1	NP_172303.5
Oryza sativa (rice)	Os02g0511900	NP_001046939.2
Caenorhabditis elegans (roundworm)	F33H2.5	NP_493616.1
Canis lupus familiaris (dog)	POLE	XP_543348.3
Mus musculus (house mouse)	Pole	NP_035262.2
Kluyveromyces lactis	KLLA0C02585g	XP_452312.1
Neurospora crassa	NCU04548	XP_955939.2
Xenopus tropicalis (tropical clawed frog)	pole	XP_004910692.1
Drosophila melanogaster (fruit fly)	DNApol-epsilon	NP_524462.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004615	XP_315205.4
Saccharomyces cerevisiae (baker's yeast)	POL2	NP_014137.1
Eremothecium gossypii	AGOS_AFR657C	NP_986205.2
Magnaporthe oryzae (rice blast fungus)	MGG_03850	XP_003720041.1
Homo sapiens (human)	POLE	NP_006222.2
Pan troglodytes (chimpanzee)	POLE	XP_522564.4
Bos taurus (cattle)	POLE	NP_001178358.1
Rattus norvegicus (Norway rat)	Pole	NP_001100622.2
Gallus gallus (chicken)	POLE	XP_004934482.1
Schizosaccharomyces pombe (fission yeast)	cdc20	NP_596354.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

POLE cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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