POLE cDNA ORF clone, Bos taurus(cattle)

The following POLE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa224428 XM_005217684.4
Latest version!
Bos taurus DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa224429 XM_024977498.1
Latest version!
Bos taurus DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa36974 NM_001191429.1
Latest version!
Bos taurus DNA polymerase epsilon, catalytic subunit (POLE), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OBa27766 XM_005217684.3
Latest version!
Bos taurus polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa224428
    Clone ID Related Accession (Same CDS sequence) XM_005217684.4
    Accession Version XM_005217684.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA polymerase epsilon catalytic subunit A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217684.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC 
    CGGGATGATG GCTCCTCTTC CTCTGTCTCG GCACTCAAGC GCCTGGAGCG CAGTCAGTGG
    ACAGATAAGA TGGATCTGCG GTTTGGTTTT GAGAGACTCA AGGAGCCTGG TGAGAAGACA
    GGCTGGCTCA TCAACATGCA CCCTACTGAG ATTTTAGATG AAGATAAACG CTTAGTTAGT
    GCTGTGGATT ACTACTTTAT TCAAGACGAT GGGAGCAGAT TTAAGGTGGC CTTGCCCTAC
    AAGCCATATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTT
    TCTAAGAAGT TTCAGGGTAA AATTGCCAAA GTAGAGACTG TCCCTAAGGA GGATCTGGAC
    TTGCCAAATC ACTTGGTTGG TTTGAAACGA AATTACATTA AGCTGTCCTT CAACACTGTG
    GAGGACCTCG TCAAAGTGAG GAAGGAGATC TCCCCAGCTG TGAAGAAAAA CCGGGACCAG
    AGTCATACCA GTGATGCCTA CACAACTATG CTCTCCAGTG TTCTGCAAGG GGGCAGTGTA
    ATTTTTGATG AAGAGGAAAC CTCTAAGAAG ATTGCCGACC AGTTGGACAA CATTGTGGAC
    ATGCGAGAGT ATGATGTACC TTACCACATC CGTCTCTCCA TCGACCTGAA GATCCACGTG
    GCTCACTGGT ACAATGTCCG GTACCGAGGA AGCGCCTTTC CAGTGGAAAT TGCCCGCCGT
    GATGACCTCG TTGAACGACC AGACCCCGTG GTTTTAGCAT TTGACATTGA GACAACCAAA
    CTGCCCCTCA AGTTTCCAGA TGCTGAGACC GACCAGATTA TGATGATTTC CTATATGATT
    GATGGTCAGG GCTACCTCAT CACCAACAGG GAGATTGTCT CAGAAGATAT AGAAGATTTC
    GAATTTACCC CCAAGCCAGA ATATGAAGGA CCCTTCTGTG TCTTCAATGA GCCCGATGAG
    GTTCATCTAA TCCAAAGATG GTTTGAACAC ATCCAGGAGG CCAAACCCAC CATCATGGTC
    ACCTATAATG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCAGAGCAGC GGTCCATGGA
    CTGAGCATGT ACCAGGAGAT AGGGTTCCAG AAGGACAGCC AGGGGGAGTA CAAATCACCC
    CAGTGTATCC ACATGGACTG TCTCAGGTGG GTGAAAAGGG ACAGTTACCT GCCCGTGGGC
    AGCCACAACC TCAAGGCGGC GGCCAAAGCC AAGCTGGGCT ACGACCCAGT GGAACTGGAC
    CCTGAAGACA TGTGCCGGAT GGCCACTGAG CAGCCCCAGA CTCTGGCGAC CTACTCGGTG
    TCGGATGCAG TGGCCACATA CTACCTGTAC ATGAAGTATG TCCACCCGTT TATATTCGCC
    CTGTGTACCA TCATCCCCAT GGAGCCCGAT GAGGTGCTGC GGAAAGGTTC TGGGACGCTG
    TGCGAGGCCT TGCTAATGGT GCAGGCCTTC CATGCCAACA TCATCTTCCC CAACAAGCAG
    GAGCAGGAGT TCAACAAGCT GACAGATGAC GGCCACGTGC TGGATGCTGA GACCTACGTG
    GGTGGCCACG TAGAAGCCCT GGAGTCCGGC GTGTTCCGCA GTGACATCCC CTGCCGGTTC
    CGGATGAATC CTGCTGCCTT TGACTTCCTG CTGCAGCGGG TGGAGAAAAC CATGCGTCAT
    GCCATCGAGG AAGAGGAGAA GGTGCCCATG GAGCAGGTCA TCAACTTCCA AGAGGTGTGT
    GATCAGATTA AGACCAAGCT CACCTCCCTG AAAGATGTTC CTAACCGAAT TGAGTGTCCC
    CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCTTGAC GAACCGCCTG
    CAGCCCTCTG CCATGGTGGA TGAGGCCACC TGCGCAGCCT GTGACTTCAA CAAGCCTGGA
    GCGAACTGCC AGAGGAAGAT GGCCTGGCAG TGGAGGGGAG AGTTCATGCC AGCCAGTCGC
    AGTGAGTATC ATCGGATCCA GCACCAACTG GAGTCAGAGA AGTTCCCTCC CTTGACCCCC
    GAGGGCCCAG CCCGGGCCTT TCACGAGCTG TCCCGTGAGG AGCAGGCTAA ATATGAGAAG
    CGGAGGCTGG CAGATTACTG CCGGAAGGCA TACAAGAAAA TCCACGTGAC CAAGGTGGAA
    GAGCGTCTCA CCACCATCTG CCAGCGGGAG AACTCCTTCT ATGTGGACAC TGTACGTGCT
    TTTCGGGACA GGCGCTATGA GTTCAAAGGT CTCCACAAGG TGTGGAAAAA GAAGCTCTCC
    GCAGCCGTGG AGGTGGGGGA CGCGGCGGAG GTGAAGCGCT GCAGGAACAT GGAGGTCCTG
    TACGACTCCC TGCAGCTGGC GCACAAGTGC ATCCTCAACT CCTTCTATGG CTACGTCATG
    CGCAAGGGGG CCCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC
    AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAAATCG GGAGGCCCTT AGAGCTGGAC
    ACAGATGGAA TATGGTGTGT CCTGCCCAAC AGCTTCCCCG AAAACTTTGT CATCAAGACC
    ACCAATGTGA AAAAGCCCAA GGTGACCATC TCTTACCCTG GGGCTATGTT AAACATCATG
    GTCAAGGAAG GCTTCACCAA TGATCAGTAC CAGGAGCTGG CTGAGCCTTC TTCACTAACC
    TATGTCACTC GCTCAGAGAA CAGCATCTTT TTCGAGGTTG ATGGGCCCTA CCTTGCCATG
    ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAACTGAAGA AGAGGTATGC CGTGTTCAAT
    GAGGATGGTT CCCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGTGG GGAGCTGCAG
    CTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTTC TTAAGGGCAG CACCCTAGAA
    GAAGTGTATG GCTCTGTCGC CAAGGTGGCT GATTACTGGC TGGACGTACT ATACAGCAAG
    GCAGCTAACA TGCCTGATTC CGAACTGTTC GAGCTGATCT CCGAGAACCG TTCCATGTCT
    CGGAAGCTGG AAGATTATGG GGAGCAGAAG TCGACATCCA TCAGCACGGC CAAGCGCCTG
    GCTGAGTTCC TGGGAGATCA GATGGTGAAG GATGCAGGGC TGAGCTGCCG CTACATCATC
    TCCCGGAAGC CTGAGGGGTC TCCAGTCACA GAGAGAGCCA TTCCACTGGC CATTTTCCAG
    GCAGAGCCCA CCGTGAGGAA GCACTTTCTC CGAAAATGGC TAAAGAGTTC TTCCCTTCAG
    GACTTTGATA TTCGGACAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGGAGTGCC
    ATACAGAAGA TCATCACGAT CCCTGCGGCC CTGCAGCAGG TGAAGAACCC AGTACCGCGT
    GTCAAACACC CTGACTGGCT GCACAAAAAA CTGTTAGAGA AAAATGACGT CTACAAGCAG
    AAGAAGATCA GTGAGCTCTT CGTCCTTGAG GGCAAGAGAC AGGTGGGCAT GGCCCAAGCT
    TCAGAAGGTT CCCAAAGCCT TGGCTCCCCC GACATGGAGG ACTTTGGCCT CACAAAGCCA
    CCTCACTCGG CAGTGCCCAT TGCTACCAAG AGAAAGCGTA TCCACTGTGA GAGGCAAGAG
    GGATCGCAGG ACCTGGAGCT GACGATGCCC TGGCAGGAGG TTTTGGGGCA GCCTCCGGCA
    CTCGGAACCA CCCAGGAAGA GTGGCTGGTC TGGCTCCAAT TCCACAAGAA GAAGTGGCAG
    CTGCAGGCTC AACAGCGCCT TGCTCACAAG AAGAGGCGGC GTCTGGAGGG GGCAGAGGGC
    ACACCAAGGC CTGGGGCCAT CCGAGAGGGG CCCTCCACAG GACTGGGGGG CTTCTTTCGA
    AGAACAGCTC GCAGCATTCT GGACCTTCCA TGGCAGATTG TGCAGATCAG TGAGACCAGC
    CAGGCTGGCT TGTTCAGGGT ATGGGCTGTC ATCGGCAGTG ACCTGCACTG CATCAAGCTG
    AACATTCCCC GGGTGTTCTA CGTGAACCAG CGGGTGGCCA AAGCGGAGGA GGGCCCTTCC
    TATCGGAAGG TGAATCGAGT CCTTCCTCGC TCCAATATGG TCTACAACCT CTACGAATAC
    TCGGTGCCAG AGGACATGTA CCAAGAACAT ATCAACGAGA TCAACACTGA GCTGGCGGCG
    CCAGACATCG AGGGCGTGTA TGAGACTCAG GTTCCATTAG TGTTTCGGGC CCTGGTGCAG
    CTGGGCTGCA TGTGTGTGGT CAGTAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA
    GAAACCTTCA CTCTTGAGCA CCTGGAGATG CGTTCCTTGG CCCAGTTCAA CTACCTGGAA
    CCAGGCAGCA TCCGCCACAT CTACCTGTAC CACCACGCAC AGGGCCACAA GGCTCTCTTT
    GGCATCTTCG TACCCTCACA GCGCAAGGCA GCTGTGTTTG TGCTGGATAC GGTGCGAAGC
    AACCAGATGC CCAGCCTCAG TGCCCTGTAC TCAACCGAGC ACAGCCTCCT GCTGGAGAAA
    GTGGGCCCTG AGCTTCTGCC CCCACAGAAA CACGCTTTTG AAGTTCGTGC TGAAACAGAC
    TTGAAGATCG TCTGCAGAGC CATCCAGCGC TTCCTGTTGA CCTACAAAGA GGAGCGCCGT
    GGGCCAACTC TCATTGCTGT TCAGTCCAAC TGGGACCTGA AGAGGCTGAC TGGCGAGATC
    CCTGTCCTGG AGGAATTCCC ACTGGTGCCT GTCCGCGTGG CTGACAAGAT CAGCTACGGG
    GTCCTGGACT GGCAGCGCCA CGGAGCCCGG CGCATGATCC GGCACTACCT CAACCTGGAC
    ACGTATCTGT CACAGGCCTT CGAGATGAGC AGGTACTTCC ACGTCCCCGT TGGGAATCTG
    CCTGAGGACA TCTCTACCTT TGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC
    AACCACCTGC TCTGGCTCTC TCCTACGTCT CGCCCAGACC TGGGTGGGAA GGAGGCCGAC
    GACAACAGGC TCGTCATGGA GTTTGATGAC CAAGCCCCTG TGGAGATCAA CAGTTCCGGC
    TGTTACTCCA CAGTGTGCGT GGAACTGGAT ATTCAGAACC TGGCCGTCAA CACCATCCTG
    CAGTCTCACC ACGTCAATGA TATGGAGGGG GCCGACAGCA TTGGCATCAG CTTCGATGTG
    ATCCAGCAGG CCTCCCTGGA GGACATGATC ACAGGCAATC AGGCTGCCAG CGCCCCTGCC
    AGCTATGACG AGACAGCCCT CTGCTCCAAC ACCTTCAGGA TCCTGAAGAG CATGGTGGTG
    GGCTGGGTGA AGGAGATCAC GCAGTACCGC AACGTCTACG CCGACAACCA GGTGATGCAC
    TTCTACCGCT GGCTCCGGTC GCCGTCCTCG CTGCTGCACG ACCCTGCCCT GCACCGCACG
    CTGCACAGCA TGATGAAGAA GCTCTTCCTT CAACTCATCG CTGAGTTCAA GCGCCTGGGG
    TCATCGGTCG TCTATGCCAA CTTCAACCGC ATCATCCTCT GCACCAAGAA GCGGCGGGTG
    GAGGACGCCC TGGCCTACGT GGAGTACATC ACCAACAGCA TCCACTCTAA AGAGATCTTC
    CATTCTTTGA CAATTTCCTT TTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCCTCT
    AATTATGGTG GAATCAAAGG AACTGTTCCA TCTAGGATTC ACTGTGGACA GGACTCCCCA
    GAAGAGGGCA GAGCACAGCA GGGGGAACCT GAGGAGGAGG AGGACGAGGA CGAGGAGGAG
    GAGGAGGAGG AGGACGAGGG AGGCACACAG GAACTGGATG TCGAGGACTT GCTGGAAAAC
    AACTGGAACA TCCTGCAGTT CCTGCCCCAG GCAGCCTCCT GCCAGAGCTA CTTCCTCATG
    ATCGTTTCAG CGTACATCGT GGCCGTGTAC CACAGCATGA AGGAGGAACT GAGGCGCAGC
    ACCCCAGGGA GCACCCCCGT GAGGAGGAGG GGAGCCAGCC AGCTCTCCCA GGAGGCCCAG
    GGGGAGGCCG GAGCCCTTCC TGGAATGATC ACCTTCTCAC AAGATTATGT GGCAAATGAA
    CTCACTCAGA ACTTCTTCAC CATCACTCAG AAGATTCAGA AGAAAGTCAC AGGCTCTCGG
    AACTCTACTG AGCTCTCAGA AATGTTTCCT GTTCTCCCCG GTTCTCACCT GCTGCTCAAT
    AACCCTGCTC TGGAGTTCAT CAAATACGTG TGCAAGGTCC TGTCTCTGGA CACCAACATC
    ACAAACCAGG TGAACAAGCT AACCCGAGAC CTGCTGCGCC TGGTAGACGT AGGCGAGTTC
    TCAGAGGAGG CCCAGTTCCG AGACCCCTGC CGTTCCTACG TGCTCCCTGA GGTCATCTGC
    CACAACTGTA ACTTCTGCCG GGACCTGGAC CTGTGCAAAG ACCCCTCCTT CTCTCAGGAT
    GGGACCGTGC TGCCTCAGTG GCTCTGCTCC AACTGTCAGG TTGCCTACGA CTCATCAGTC
    ATTGAGATGG CCCTGGTGGA AGCCCTACAG AAGAAGCTGA TGGCCTTTAC CCTGCAGGAC
    CTGGTCTGCC TGAAGTGCCG GGGTGTGAAG GAGACCAACA TGGCCGTGTA CTGCAGCTGT
    GCGGGGGGCT TCTCCCTCAC CATCCACACT AAGGTCTTCA TGGAACAGAT CGGAATCTTC
    CAGAACATCG CCCAGCACTA TGGCATGTCA TACCTCAAGG AGACCCTGGA TTGGCTGCAG
    CTTCAGAACC CCCAAGTGGG CCATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217741.1
    CDS80..6946
    Translation

    Target ORF information:

    RefSeq Version XM_005217684.4
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217684.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC 
    CGGGATGATG GCTCCTCTTC CTCTGTCTCG GCACTCAAGC GCCTGGAGCG CAGTCAGTGG
    ACAGATAAGA TGGATCTGCG GTTTGGTTTT GAGAGACTCA AGGAGCCTGG TGAGAAGACA
    GGCTGGCTCA TCAACATGCA CCCTACTGAG ATTTTAGATG AAGATAAACG CTTAGTTAGT
    GCTGTGGATT ACTACTTTAT TCAAGACGAT GGGAGCAGAT TTAAGGTGGC CTTGCCCTAC
    AAGCCATATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTT
    TCTAAGAAGT TTCAGGGTAA AATTGCCAAA GTAGAGACTG TCCCTAAGGA GGATCTGGAC
    TTGCCAAATC ACTTGGTTGG TTTGAAACGA AATTACATTA AGCTGTCCTT CAACACTGTG
    GAGGACCTCG TCAAAGTGAG GAAGGAGATC TCCCCAGCTG TGAAGAAAAA CCGGGACCAG
    AGTCATACCA GTGATGCCTA CACAACTATG CTCTCCAGTG TTCTGCAAGG GGGCAGTGTA
    ATTTTTGATG AAGAGGAAAC CTCTAAGAAG ATTGCCGACC AGTTGGACAA CATTGTGGAC
    ATGCGAGAGT ATGATGTACC TTACCACATC CGTCTCTCCA TCGACCTGAA GATCCACGTG
    GCTCACTGGT ACAATGTCCG GTACCGAGGA AGCGCCTTTC CAGTGGAAAT TGCCCGCCGT
    GATGACCTCG TTGAACGACC AGACCCCGTG GTTTTAGCAT TTGACATTGA GACAACCAAA
    CTGCCCCTCA AGTTTCCAGA TGCTGAGACC GACCAGATTA TGATGATTTC CTATATGATT
    GATGGTCAGG GCTACCTCAT CACCAACAGG GAGATTGTCT CAGAAGATAT AGAAGATTTC
    GAATTTACCC CCAAGCCAGA ATATGAAGGA CCCTTCTGTG TCTTCAATGA GCCCGATGAG
    GTTCATCTAA TCCAAAGATG GTTTGAACAC ATCCAGGAGG CCAAACCCAC CATCATGGTC
    ACCTATAATG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCAGAGCAGC GGTCCATGGA
    CTGAGCATGT ACCAGGAGAT AGGGTTCCAG AAGGACAGCC AGGGGGAGTA CAAATCACCC
    CAGTGTATCC ACATGGACTG TCTCAGGTGG GTGAAAAGGG ACAGTTACCT GCCCGTGGGC
    AGCCACAACC TCAAGGCGGC GGCCAAAGCC AAGCTGGGCT ACGACCCAGT GGAACTGGAC
    CCTGAAGACA TGTGCCGGAT GGCCACTGAG CAGCCCCAGA CTCTGGCGAC CTACTCGGTG
    TCGGATGCAG TGGCCACATA CTACCTGTAC ATGAAGTATG TCCACCCGTT TATATTCGCC
    CTGTGTACCA TCATCCCCAT GGAGCCCGAT GAGGTGCTGC GGAAAGGTTC TGGGACGCTG
    TGCGAGGCCT TGCTAATGGT GCAGGCCTTC CATGCCAACA TCATCTTCCC CAACAAGCAG
    GAGCAGGAGT TCAACAAGCT GACAGATGAC GGCCACGTGC TGGATGCTGA GACCTACGTG
    GGTGGCCACG TAGAAGCCCT GGAGTCCGGC GTGTTCCGCA GTGACATCCC CTGCCGGTTC
    CGGATGAATC CTGCTGCCTT TGACTTCCTG CTGCAGCGGG TGGAGAAAAC CATGCGTCAT
    GCCATCGAGG AAGAGGAGAA GGTGCCCATG GAGCAGGTCA TCAACTTCCA AGAGGTGTGT
    GATCAGATTA AGACCAAGCT CACCTCCCTG AAAGATGTTC CTAACCGAAT TGAGTGTCCC
    CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCTTGAC GAACCGCCTG
    CAGCCCTCTG CCATGGTGGA TGAGGCCACC TGCGCAGCCT GTGACTTCAA CAAGCCTGGA
    GCGAACTGCC AGAGGAAGAT GGCCTGGCAG TGGAGGGGAG AGTTCATGCC AGCCAGTCGC
    AGTGAGTATC ATCGGATCCA GCACCAACTG GAGTCAGAGA AGTTCCCTCC CTTGACCCCC
    GAGGGCCCAG CCCGGGCCTT TCACGAGCTG TCCCGTGAGG AGCAGGCTAA ATATGAGAAG
    CGGAGGCTGG CAGATTACTG CCGGAAGGCA TACAAGAAAA TCCACGTGAC CAAGGTGGAA
    GAGCGTCTCA CCACCATCTG CCAGCGGGAG AACTCCTTCT ATGTGGACAC TGTACGTGCT
    TTTCGGGACA GGCGCTATGA GTTCAAAGGT CTCCACAAGG TGTGGAAAAA GAAGCTCTCC
    GCAGCCGTGG AGGTGGGGGA CGCGGCGGAG GTGAAGCGCT GCAGGAACAT GGAGGTCCTG
    TACGACTCCC TGCAGCTGGC GCACAAGTGC ATCCTCAACT CCTTCTATGG CTACGTCATG
    CGCAAGGGGG CCCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC
    AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAAATCG GGAGGCCCTT AGAGCTGGAC
    ACAGATGGAA TATGGTGTGT CCTGCCCAAC AGCTTCCCCG AAAACTTTGT CATCAAGACC
    ACCAATGTGA AAAAGCCCAA GGTGACCATC TCTTACCCTG GGGCTATGTT AAACATCATG
    GTCAAGGAAG GCTTCACCAA TGATCAGTAC CAGGAGCTGG CTGAGCCTTC TTCACTAACC
    TATGTCACTC GCTCAGAGAA CAGCATCTTT TTCGAGGTTG ATGGGCCCTA CCTTGCCATG
    ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAACTGAAGA AGAGGTATGC CGTGTTCAAT
    GAGGATGGTT CCCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGTGG GGAGCTGCAG
    CTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTTC TTAAGGGCAG CACCCTAGAA
    GAAGTGTATG GCTCTGTCGC CAAGGTGGCT GATTACTGGC TGGACGTACT ATACAGCAAG
    GCAGCTAACA TGCCTGATTC CGAACTGTTC GAGCTGATCT CCGAGAACCG TTCCATGTCT
    CGGAAGCTGG AAGATTATGG GGAGCAGAAG TCGACATCCA TCAGCACGGC CAAGCGCCTG
    GCTGAGTTCC TGGGAGATCA GATGGTGAAG GATGCAGGGC TGAGCTGCCG CTACATCATC
    TCCCGGAAGC CTGAGGGGTC TCCAGTCACA GAGAGAGCCA TTCCACTGGC CATTTTCCAG
    GCAGAGCCCA CCGTGAGGAA GCACTTTCTC CGAAAATGGC TAAAGAGTTC TTCCCTTCAG
    GACTTTGATA TTCGGACAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGGAGTGCC
    ATACAGAAGA TCATCACGAT CCCTGCGGCC CTGCAGCAGG TGAAGAACCC AGTACCGCGT
    GTCAAACACC CTGACTGGCT GCACAAAAAA CTGTTAGAGA AAAATGACGT CTACAAGCAG
    AAGAAGATCA GTGAGCTCTT CGTCCTTGAG GGCAAGAGAC AGGTGGGCAT GGCCCAAGCT
    TCAGAAGGTT CCCAAAGCCT TGGCTCCCCC GACATGGAGG ACTTTGGCCT CACAAAGCCA
    CCTCACTCGG CAGTGCCCAT TGCTACCAAG AGAAAGCGTA TCCACTGTGA GAGGCAAGAG
    GGATCGCAGG ACCTGGAGCT GACGATGCCC TGGCAGGAGG TTTTGGGGCA GCCTCCGGCA
    CTCGGAACCA CCCAGGAAGA GTGGCTGGTC TGGCTCCAAT TCCACAAGAA GAAGTGGCAG
    CTGCAGGCTC AACAGCGCCT TGCTCACAAG AAGAGGCGGC GTCTGGAGGG GGCAGAGGGC
    ACACCAAGGC CTGGGGCCAT CCGAGAGGGG CCCTCCACAG GACTGGGGGG CTTCTTTCGA
    AGAACAGCTC GCAGCATTCT GGACCTTCCA TGGCAGATTG TGCAGATCAG TGAGACCAGC
    CAGGCTGGCT TGTTCAGGGT ATGGGCTGTC ATCGGCAGTG ACCTGCACTG CATCAAGCTG
    AACATTCCCC GGGTGTTCTA CGTGAACCAG CGGGTGGCCA AAGCGGAGGA GGGCCCTTCC
    TATCGGAAGG TGAATCGAGT CCTTCCTCGC TCCAATATGG TCTACAACCT CTACGAATAC
    TCGGTGCCAG AGGACATGTA CCAAGAACAT ATCAACGAGA TCAACACTGA GCTGGCGGCG
    CCAGACATCG AGGGCGTGTA TGAGACTCAG GTTCCATTAG TGTTTCGGGC CCTGGTGCAG
    CTGGGCTGCA TGTGTGTGGT CAGTAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA
    GAAACCTTCA CTCTTGAGCA CCTGGAGATG CGTTCCTTGG CCCAGTTCAA CTACCTGGAA
    CCAGGCAGCA TCCGCCACAT CTACCTGTAC CACCACGCAC AGGGCCACAA GGCTCTCTTT
    GGCATCTTCG TACCCTCACA GCGCAAGGCA GCTGTGTTTG TGCTGGATAC GGTGCGAAGC
    AACCAGATGC CCAGCCTCAG TGCCCTGTAC TCAACCGAGC ACAGCCTCCT GCTGGAGAAA
    GTGGGCCCTG AGCTTCTGCC CCCACAGAAA CACGCTTTTG AAGTTCGTGC TGAAACAGAC
    TTGAAGATCG TCTGCAGAGC CATCCAGCGC TTCCTGTTGA CCTACAAAGA GGAGCGCCGT
    GGGCCAACTC TCATTGCTGT TCAGTCCAAC TGGGACCTGA AGAGGCTGAC TGGCGAGATC
    CCTGTCCTGG AGGAATTCCC ACTGGTGCCT GTCCGCGTGG CTGACAAGAT CAGCTACGGG
    GTCCTGGACT GGCAGCGCCA CGGAGCCCGG CGCATGATCC GGCACTACCT CAACCTGGAC
    ACGTATCTGT CACAGGCCTT CGAGATGAGC AGGTACTTCC ACGTCCCCGT TGGGAATCTG
    CCTGAGGACA TCTCTACCTT TGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC
    AACCACCTGC TCTGGCTCTC TCCTACGTCT CGCCCAGACC TGGGTGGGAA GGAGGCCGAC
    GACAACAGGC TCGTCATGGA GTTTGATGAC CAAGCCCCTG TGGAGATCAA CAGTTCCGGC
    TGTTACTCCA CAGTGTGCGT GGAACTGGAT ATTCAGAACC TGGCCGTCAA CACCATCCTG
    CAGTCTCACC ACGTCAATGA TATGGAGGGG GCCGACAGCA TTGGCATCAG CTTCGATGTG
    ATCCAGCAGG CCTCCCTGGA GGACATGATC ACAGGCAATC AGGCTGCCAG CGCCCCTGCC
    AGCTATGACG AGACAGCCCT CTGCTCCAAC ACCTTCAGGA TCCTGAAGAG CATGGTGGTG
    GGCTGGGTGA AGGAGATCAC GCAGTACCGC AACGTCTACG CCGACAACCA GGTGATGCAC
    TTCTACCGCT GGCTCCGGTC GCCGTCCTCG CTGCTGCACG ACCCTGCCCT GCACCGCACG
    CTGCACAGCA TGATGAAGAA GCTCTTCCTT CAACTCATCG CTGAGTTCAA GCGCCTGGGG
    TCATCGGTCG TCTATGCCAA CTTCAACCGC ATCATCCTCT GCACCAAGAA GCGGCGGGTG
    GAGGACGCCC TGGCCTACGT GGAGTACATC ACCAACAGCA TCCACTCTAA AGAGATCTTC
    CATTCTTTGA CAATTTCCTT TTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCCTCT
    AATTATGGTG GAATCAAAGG AACTGTTCCA TCTAGGATTC ACTGTGGACA GGACTCCCCA
    GAAGAGGGCA GAGCACAGCA GGGGGAACCT GAGGAGGAGG AGGACGAGGA CGAGGAGGAG
    GAGGAGGAGG AGGACGAGGG AGGCACACAG GAACTGGATG TCGAGGACTT GCTGGAAAAC
    AACTGGAACA TCCTGCAGTT CCTGCCCCAG GCAGCCTCCT GCCAGAGCTA CTTCCTCATG
    ATCGTTTCAG CGTACATCGT GGCCGTGTAC CACAGCATGA AGGAGGAACT GAGGCGCAGC
    ACCCCAGGGA GCACCCCCGT GAGGAGGAGG GGAGCCAGCC AGCTCTCCCA GGAGGCCCAG
    GGGGAGGCCG GAGCCCTTCC TGGAATGATC ACCTTCTCAC AAGATTATGT GGCAAATGAA
    CTCACTCAGA ACTTCTTCAC CATCACTCAG AAGATTCAGA AGAAAGTCAC AGGCTCTCGG
    AACTCTACTG AGCTCTCAGA AATGTTTCCT GTTCTCCCCG GTTCTCACCT GCTGCTCAAT
    AACCCTGCTC TGGAGTTCAT CAAATACGTG TGCAAGGTCC TGTCTCTGGA CACCAACATC
    ACAAACCAGG TGAACAAGCT AACCCGAGAC CTGCTGCGCC TGGTAGACGT AGGCGAGTTC
    TCAGAGGAGG CCCAGTTCCG AGACCCCTGC CGTTCCTACG TGCTCCCTGA GGTCATCTGC
    CACAACTGTA ACTTCTGCCG GGACCTGGAC CTGTGCAAAG ACCCCTCCTT CTCTCAGGAT
    GGGACCGTGC TGCCTCAGTG GCTCTGCTCC AACTGTCAGG TTGCCTACGA CTCATCAGTC
    ATTGAGATGG CCCTGGTGGA AGCCCTACAG AAGAAGCTGA TGGCCTTTAC CCTGCAGGAC
    CTGGTCTGCC TGAAGTGCCG GGGTGTGAAG GAGACCAACA TGGCCGTGTA CTGCAGCTGT
    GCGGGGGGCT TCTCCCTCAC CATCCACACT AAGGTCTTCA TGGAACAGAT CGGAATCTTC
    CAGAACATCG CCCAGCACTA TGGCATGTCA TACCTCAAGG AGACCCTGGA TTGGCTGCAG
    CTTCAGAACC CCCAAGTGGG CCATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224429
    Clone ID Related Accession (Same CDS sequence) XM_024977498.1
    Accession Version XM_024977498.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA polymerase epsilon catalytic subunit A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGATCTGC GGTTTGGTTT TGAGAGACTC AAGGAGCCTG GTGAGAAGAC AGGCTGGCTC 
    ATCAACATGC ACCCTACTGA GATTTTAGAT GAAGATAAAC GCTTAGTTAG TGCTGTGGAT
    TACTACTTTA TTCAAGACGA TGGGAGCAGA TTTAAGGTGG CCTTGCCCTA CAAGCCATAT
    TTCTACATTG CGACCAGAAA GGGTTGTGAG CGAGAAGTTT CATCTTTTCT TTCTAAGAAG
    TTTCAGGGTA AAATTGCCAA AGTAGAGACT GTCCCTAAGG AGGATCTGGA CTTGCCAAAT
    CACTTGGTTG GTTTGAAACG AAATTACATT AAGCTGTCCT TCAACACTGT GGAGGACCTC
    GTCAAAGTGA GGAAGGAGAT CTCCCCAGCT GTGAAGAAAA ACCGGGACCA GAGTCATACC
    AGTGATGCCT ACACAACTAT GCTCTCCAGT GTTCTGCAAG GGGGCAGTGT AATTTTTGAT
    GAAGAGGAAA CCTCTAAGAA GATTGCCGAC CAGTTGGACA ACATTGTGGA CATGCGAGAG
    TATGATGTAC CTTACCACAT CCGTCTCTCC ATCGACCTGA AGATCCACGT GGCTCACTGG
    TACAATGTCC GGTACCGAGG AAGCGCCTTT CCAGTGGAAA TTGCCCGCCG TGATGACCTC
    GTTGAACGAC CAGACCCCGT GGTTTTAGCA TTTGACATTG AGACAACCAA ACTGCCCCTC
    AAGTTTCCAG ATGCTGAGAC CGACCAGATT ATGATGATTT CCTATATGAT TGATGGTCAG
    GGCTACCTCA TCACCAACAG GGAGATTGTC TCAGAAGATA TAGAAGATTT CGAATTTACC
    CCCAAGCCAG AATATGAAGG ACCCTTCTGT GTCTTCAATG AGCCCGATGA GGTTCATCTA
    ATCCAAAGAT GGTTTGAACA CATCCAGGAG GCCAAACCCA CCATCATGGT CACCTATAAT
    GGGGACTTTT TTGACTGGCC ATTTGTGGAG GCCAGAGCAG CGGTCCATGG ACTGAGCATG
    TACCAGGAGA TAGGGTTCCA GAAGGACAGC CAGGGGGAGT ACAAATCACC CCAGTGTATC
    CACATGGACT GTCTCAGGTG GGTGAAAAGG GACAGTTACC TGCCCGTGGG CAGCCACAAC
    CTCAAGGCGG CGGCCAAAGC CAAGCTGGGC TACGACCCAG TGGAACTGGA CCCTGAAGAC
    ATGTGCCGGA TGGCCACTGA GCAGCCCCAG ACTCTGGCGA CCTACTCGGT GTCGGATGCA
    GTGGCCACAT ACTACCTGTA CATGAAGTAT GTCCACCCGT TTATATTCGC CCTGTGTACC
    ATCATCCCCA TGGAGCCCGA TGAGGTGCTG CGGAAAGGTT CTGGGACGCT GTGCGAGGCC
    TTGCTAATGG TGCAGGCCTT CCATGCCAAC ATCATCTTCC CCAACAAGCA GGAGCAGGAG
    TTCAACAAGC TGACAGATGA CGGCCACGTG CTGGATGCTG AGACCTACGT GGGTGGCCAC
    GTAGAAGCCC TGGAGTCCGG CGTGTTCCGC AGTGACATCC CCTGCCGGTT CCGGATGAAT
    CCTGCTGCCT TTGACTTCCT GCTGCAGCGG GTGGAGAAAA CCATGCGTCA TGCCATCGAG
    GAAGAGGAGA AGGTGCCCAT GGAGCAGGTC ATCAACTTCC AAGAGGTGTG TGATCAGATT
    AAGACCAAGC TCACCTCCCT GAAAGATGTT CCTAACCGAA TTGAGTGTCC CCTCATCTAC
    CACCTGGACG TGGGGGCCAT GTACCCCAAC ATCATCTTGA CGAACCGCCT GCAGCCCTCT
    GCCATGGTGG ATGAGGCCAC CTGCGCAGCC TGTGACTTCA ACAAGCCTGG AGCGAACTGC
    CAGAGGAAGA TGGCCTGGCA GTGGAGGGGA GAGTTCATGC CAGCCAGTCG CAGTGAGTAT
    CATCGGATCC AGCACCAACT GGAGTCAGAG AAGTTCCCTC CCTTGACCCC CGAGGGCCCA
    GCCCGGGCCT TTCACGAGCT GTCCCGTGAG GAGCAGGCTA AATATGAGAA GCGGAGGCTG
    GCAGATTACT GCCGGAAGGC ATACAAGAAA ATCCACGTGA CCAAGGTGGA AGAGCGTCTC
    ACCACCATCT GCCAGCGGGA GAACTCCTTC TATGTGGACA CTGTACGTGC TTTTCGGGAC
    AGGCGCTATG AGTTCAAAGG TCTCCACAAG GTGTGGAAAA AGAAGCTCTC CGCAGCCGTG
    GAGGTGGGGG ACGCGGCGGA GGTGAAGCGC TGCAGGAACA TGGAGGTCCT GTACGACTCC
    CTGCAGCTGG CGCACAAGTG CATCCTCAAC TCCTTCTATG GCTACGTCAT GCGCAAGGGG
    GCCCGCTGGT ACTCCATGGA GATGGCTGGC ATCGTCTGCT TCACAGGGGC CAACATCATC
    ACCCAGGCAC GGGAGCTGAT CGAGCAAATC GGGAGGCCCT TAGAGCTGGA CACAGATGGA
    ATATGGTGTG TCCTGCCCAA CAGCTTCCCC GAAAACTTTG TCATCAAGAC CACCAATGTG
    AAAAAGCCCA AGGTGACCAT CTCTTACCCT GGGGCTATGT TAAACATCAT GGTCAAGGAA
    GGCTTCACCA ATGATCAGTA CCAGGAGCTG GCTGAGCCTT CTTCACTAAC CTATGTCACT
    CGCTCAGAGA ACAGCATCTT TTTCGAGGTT GATGGGCCCT ACCTTGCCAT GATCCTTCCA
    GCCTCCAAGG AAGAAGGCAA GAAACTGAAG AAGAGGTATG CCGTGTTCAA TGAGGATGGT
    TCCCTGGCTG AGCTCAAGGG CTTTGAGGTC AAACGCCGTG GGGAGCTGCA GCTGATTAAA
    ATCTTCCAGT CCTCAGTGTT TGAGGCCTTT CTTAAGGGCA GCACCCTAGA AGAAGTGTAT
    GGCTCTGTCG CCAAGGTGGC TGATTACTGG CTGGACGTAC TATACAGCAA GGCAGCTAAC
    ATGCCTGATT CCGAACTGTT CGAGCTGATC TCCGAGAACC GTTCCATGTC TCGGAAGCTG
    GAAGATTATG GGGAGCAGAA GTCGACATCC ATCAGCACGG CCAAGCGCCT GGCTGAGTTC
    CTGGGAGATC AGATGGTGAA GGATGCAGGG CTGAGCTGCC GCTACATCAT CTCCCGGAAG
    CCTGAGGGGT CTCCAGTCAC AGAGAGAGCC ATTCCACTGG CCATTTTCCA GGCAGAGCCC
    ACCGTGAGGA AGCACTTTCT CCGAAAATGG CTAAAGAGTT CTTCCCTTCA GGACTTTGAT
    ATTCGGACAA TTCTGGATTG GGACTACTAC ATTGAGCGGC TGGGGAGTGC CATACAGAAG
    ATCATCACGA TCCCTGCGGC CCTGCAGCAG GTGAAGAACC CAGTACCGCG TGTCAAACAC
    CCTGACTGGC TGCACAAAAA ACTGTTAGAG AAAAATGACG TCTACAAGCA GAAGAAGATC
    AGTGAGCTCT TCGTCCTTGA GGGCAAGAGA CAGGTGGGCA TGGCCCAAGC TTCAGAAGGT
    TCCCAAAGCC TTGGCTCCCC CGACATGGAG GACTTTGGCC TCACAAAGCC ACCTCACTCG
    GCAGTGCCCA TTGCTACCAA GAGAAAGCGT ATCCACTGTG AGAGGCAAGA GGGATCGCAG
    GACCTGGAGC TGACGATGCC CTGGCAGGAG GTTTTGGGGC AGCCTCCGGC ACTCGGAACC
    ACCCAGGAAG AGTGGCTGGT CTGGCTCCAA TTCCACAAGA AGAAGTGGCA GCTGCAGGCT
    CAACAGCGCC TTGCTCACAA GAAGAGGCGG CGTCTGGAGG GGGCAGAGGG CACACCAAGG
    CCTGGGGCCA TCCGAGAGGG GCCCTCCACA GGACTGGGGG GCTTCTTTCG AAGAACAGCT
    CGCAGCATTC TGGACCTTCC ATGGCAGATT GTGCAGATCA GTGAGACCAG CCAGGCTGGC
    TTGTTCAGGG TATGGGCTGT CATCGGCAGT GACCTGCACT GCATCAAGCT GAACATTCCC
    CGGGTGTTCT ACGTGAACCA GCGGGTGGCC AAAGCGGAGG AGGGCCCTTC CTATCGGAAG
    GTGAATCGAG TCCTTCCTCG CTCCAATATG GTCTACAACC TCTACGAATA CTCGGTGCCA
    GAGGACATGT ACCAAGAACA TATCAACGAG ATCAACACTG AGCTGGCGGC GCCAGACATC
    GAGGGCGTGT ATGAGACTCA GGTTCCATTA GTGTTTCGGG CCCTGGTGCA GCTGGGCTGC
    ATGTGTGTGG TCAGTAAACA GCTGGTGAGG CACCTTTCAG GCTGGGAAGC AGAAACCTTC
    ACTCTTGAGC ACCTGGAGAT GCGTTCCTTG GCCCAGTTCA ACTACCTGGA ACCAGGCAGC
    ATCCGCCACA TCTACCTGTA CCACCACGCA CAGGGCCACA AGGCTCTCTT TGGCATCTTC
    GTACCCTCAC AGCGCAAGGC AGCTGTGTTT GTGCTGGATA CGGTGCGAAG CAACCAGATG
    CCCAGCCTCA GTGCCCTGTA CTCAACCGAG CACAGCCTCC TGCTGGAGAA AGTGGGCCCT
    GAGCTTCTGC CCCCACAGAA ACACGCTTTT GAAGTTCGTG CTGAAACAGA CTTGAAGATC
    GTCTGCAGAG CCATCCAGCG CTTCCTGTTG ACCTACAAAG AGGAGCGCCG TGGGCCAACT
    CTCATTGCTG TTCAGTCCAA CTGGGACCTG AAGAGGCTGA CTGGCGAGAT CCCTGTCCTG
    GAGGAATTCC CACTGGTGCC TGTCCGCGTG GCTGACAAGA TCAGCTACGG GGTCCTGGAC
    TGGCAGCGCC ACGGAGCCCG GCGCATGATC CGGCACTACC TCAACCTGGA CACGTATCTG
    TCACAGGCCT TCGAGATGAG CAGGTACTTC CACGTCCCCG TTGGGAATCT GCCTGAGGAC
    ATCTCTACCT TTGGCTCCGA CCTCTTCTTT GCCCGCCACC TCCAGCGCCA CAACCACCTG
    CTCTGGCTCT CTCCTACGTC TCGCCCAGAC CTGGGTGGGA AGGAGGCCGA CGACAACAGG
    CTCGTCATGG AGTTTGATGA CCAAGCCCCT GTGGAGATCA ACAGTTCCGG CTGTTACTCC
    ACAGTGTGCG TGGAACTGGA TATTCAGAAC CTGGCCGTCA ACACCATCCT GCAGTCTCAC
    CACGTCAATG ATATGGAGGG GGCCGACAGC ATTGGCATCA GCTTCGATGT GATCCAGCAG
    GCCTCCCTGG AGGACATGAT CACAGGCAAT CAGGCTGCCA GCGCCCCTGC CAGCTATGAC
    GAGACAGCCC TCTGCTCCAA CACCTTCAGG ATCCTGAAGA GCATGGTGGT GGGCTGGGTG
    AAGGAGATCA CGCAGTACCG CAACGTCTAC GCCGACAACC AGGTGATGCA CTTCTACCGC
    TGGCTCCGGT CGCCGTCCTC GCTGCTGCAC GACCCTGCCC TGCACCGCAC GCTGCACAGC
    ATGATGAAGA AGCTCTTCCT TCAACTCATC GCTGAGTTCA AGCGCCTGGG GTCATCGGTC
    GTCTATGCCA ACTTCAACCG CATCATCCTC TGCACCAAGA AGCGGCGGGT GGAGGACGCC
    CTGGCCTACG TGGAGTACAT CACCAACAGC ATCCACTCTA AAGAGATCTT CCATTCTTTG
    ACAATTTCCT TTTCTCGATG CTGGGAATTT CTTCTCTGGA TGGATCCCTC TAATTATGGT
    GGAATCAAAG GAACTGTTCC ATCTAGGATT CACTGTGGAC AGCAGGACTC CCCAGAAGAG
    GGCAGAGCAC AGCAGGGGGA ACCTGAGGAG GAGGAGGACG AGGACGAGGA GGAGGAGGAG
    GAGGAGGACG AGGGAGGCAC ACAGGAACTG GATGTCGAGG ACTTGCTGGA AAACAACTGG
    AACATCCTGC AGTTCCTGCC CCAGGCAGCC TCCTGCCAGA GCTACTTCCT CATGATCGTT
    TCAGCGTACA TCGTGGCCGT GTACCACAGC ATGAAGGAGG AACTGAGGCG CAGCACCCCA
    GGGAGCACCC CCGTGAGGAG GAGGGGAGCC AGCCAGCTCT CCCAGGAGGC CCAGGGGGAG
    GCCGGAGCCC TTCCTGGAAT GATCACCTTC TCACAAGATT ATGTGGCAAA TGAACTCACT
    CAGAACTTCT TCACCATCAC TCAGAAGATT CAGAAGAAAG TCACAGGCTC TCGGAACTCT
    ACTGAGCTCT CAGAAATGTT TCCTGTTCTC CCCGGTTCTC ACCTGCTGCT CAATAACCCT
    GCTCTGGAGT TCATCAAATA CGTGTGCAAG GTCCTGTCTC TGGACACCAA CATCACAAAC
    CAGGTGAACA AGCTAACCCG AGACCTGCTG CGCCTGGTAG ACGTAGGCGA GTTCTCAGAG
    GAGGCCCAGT TCCGAGACCC CTGCCGTTCC TACGTGCTCC CTGAGGTCAT CTGCCACAAC
    TGTAACTTCT GCCGGGACCT GGACCTGTGC AAAGACCCCT CCTTCTCTCA GGATGGGACC
    GTGCTGCCTC AGTGGCTCTG CTCCAACTGT CAGGTTGCCT ACGACTCATC AGTCATTGAG
    ATGGCCCTGG TGGAAGCCCT ACAGAAGAAG CTGATGGCCT TTACCCTGCA GGACCTGGTC
    TGCCTGAAGT GCCGGGGTGT GAAGGAGACC AACATGGCCG TGTACTGCAG CTGTGCGGGG
    GGCTTCTCCC TCACCATCCA CACTAAGGTC TTCATGGAAC AGATCGGAAT CTTCCAGAAC
    ATCGCCCAGC ACTATGGCAT GTCATACCTC AAGGAGACCC TGGATTGGCT GCAGCTTCAG
    AACCCCCAAG TGGGCCATTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833266.1
    CDS153..6893
    Translation

    Target ORF information:

    RefSeq Version XM_024977498.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA polymerase epsilon, catalytic subunit (POLE), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977498.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGATCTGC GGTTTGGTTT TGAGAGACTC AAGGAGCCTG GTGAGAAGAC AGGCTGGCTC 
    ATCAACATGC ACCCTACTGA GATTTTAGAT GAAGATAAAC GCTTAGTTAG TGCTGTGGAT
    TACTACTTTA TTCAAGACGA TGGGAGCAGA TTTAAGGTGG CCTTGCCCTA CAAGCCATAT
    TTCTACATTG CGACCAGAAA GGGTTGTGAG CGAGAAGTTT CATCTTTTCT TTCTAAGAAG
    TTTCAGGGTA AAATTGCCAA AGTAGAGACT GTCCCTAAGG AGGATCTGGA CTTGCCAAAT
    CACTTGGTTG GTTTGAAACG AAATTACATT AAGCTGTCCT TCAACACTGT GGAGGACCTC
    GTCAAAGTGA GGAAGGAGAT CTCCCCAGCT GTGAAGAAAA ACCGGGACCA GAGTCATACC
    AGTGATGCCT ACACAACTAT GCTCTCCAGT GTTCTGCAAG GGGGCAGTGT AATTTTTGAT
    GAAGAGGAAA CCTCTAAGAA GATTGCCGAC CAGTTGGACA ACATTGTGGA CATGCGAGAG
    TATGATGTAC CTTACCACAT CCGTCTCTCC ATCGACCTGA AGATCCACGT GGCTCACTGG
    TACAATGTCC GGTACCGAGG AAGCGCCTTT CCAGTGGAAA TTGCCCGCCG TGATGACCTC
    GTTGAACGAC CAGACCCCGT GGTTTTAGCA TTTGACATTG AGACAACCAA ACTGCCCCTC
    AAGTTTCCAG ATGCTGAGAC CGACCAGATT ATGATGATTT CCTATATGAT TGATGGTCAG
    GGCTACCTCA TCACCAACAG GGAGATTGTC TCAGAAGATA TAGAAGATTT CGAATTTACC
    CCCAAGCCAG AATATGAAGG ACCCTTCTGT GTCTTCAATG AGCCCGATGA GGTTCATCTA
    ATCCAAAGAT GGTTTGAACA CATCCAGGAG GCCAAACCCA CCATCATGGT CACCTATAAT
    GGGGACTTTT TTGACTGGCC ATTTGTGGAG GCCAGAGCAG CGGTCCATGG ACTGAGCATG
    TACCAGGAGA TAGGGTTCCA GAAGGACAGC CAGGGGGAGT ACAAATCACC CCAGTGTATC
    CACATGGACT GTCTCAGGTG GGTGAAAAGG GACAGTTACC TGCCCGTGGG CAGCCACAAC
    CTCAAGGCGG CGGCCAAAGC CAAGCTGGGC TACGACCCAG TGGAACTGGA CCCTGAAGAC
    ATGTGCCGGA TGGCCACTGA GCAGCCCCAG ACTCTGGCGA CCTACTCGGT GTCGGATGCA
    GTGGCCACAT ACTACCTGTA CATGAAGTAT GTCCACCCGT TTATATTCGC CCTGTGTACC
    ATCATCCCCA TGGAGCCCGA TGAGGTGCTG CGGAAAGGTT CTGGGACGCT GTGCGAGGCC
    TTGCTAATGG TGCAGGCCTT CCATGCCAAC ATCATCTTCC CCAACAAGCA GGAGCAGGAG
    TTCAACAAGC TGACAGATGA CGGCCACGTG CTGGATGCTG AGACCTACGT GGGTGGCCAC
    GTAGAAGCCC TGGAGTCCGG CGTGTTCCGC AGTGACATCC CCTGCCGGTT CCGGATGAAT
    CCTGCTGCCT TTGACTTCCT GCTGCAGCGG GTGGAGAAAA CCATGCGTCA TGCCATCGAG
    GAAGAGGAGA AGGTGCCCAT GGAGCAGGTC ATCAACTTCC AAGAGGTGTG TGATCAGATT
    AAGACCAAGC TCACCTCCCT GAAAGATGTT CCTAACCGAA TTGAGTGTCC CCTCATCTAC
    CACCTGGACG TGGGGGCCAT GTACCCCAAC ATCATCTTGA CGAACCGCCT GCAGCCCTCT
    GCCATGGTGG ATGAGGCCAC CTGCGCAGCC TGTGACTTCA ACAAGCCTGG AGCGAACTGC
    CAGAGGAAGA TGGCCTGGCA GTGGAGGGGA GAGTTCATGC CAGCCAGTCG CAGTGAGTAT
    CATCGGATCC AGCACCAACT GGAGTCAGAG AAGTTCCCTC CCTTGACCCC CGAGGGCCCA
    GCCCGGGCCT TTCACGAGCT GTCCCGTGAG GAGCAGGCTA AATATGAGAA GCGGAGGCTG
    GCAGATTACT GCCGGAAGGC ATACAAGAAA ATCCACGTGA CCAAGGTGGA AGAGCGTCTC
    ACCACCATCT GCCAGCGGGA GAACTCCTTC TATGTGGACA CTGTACGTGC TTTTCGGGAC
    AGGCGCTATG AGTTCAAAGG TCTCCACAAG GTGTGGAAAA AGAAGCTCTC CGCAGCCGTG
    GAGGTGGGGG ACGCGGCGGA GGTGAAGCGC TGCAGGAACA TGGAGGTCCT GTACGACTCC
    CTGCAGCTGG CGCACAAGTG CATCCTCAAC TCCTTCTATG GCTACGTCAT GCGCAAGGGG
    GCCCGCTGGT ACTCCATGGA GATGGCTGGC ATCGTCTGCT TCACAGGGGC CAACATCATC
    ACCCAGGCAC GGGAGCTGAT CGAGCAAATC GGGAGGCCCT TAGAGCTGGA CACAGATGGA
    ATATGGTGTG TCCTGCCCAA CAGCTTCCCC GAAAACTTTG TCATCAAGAC CACCAATGTG
    AAAAAGCCCA AGGTGACCAT CTCTTACCCT GGGGCTATGT TAAACATCAT GGTCAAGGAA
    GGCTTCACCA ATGATCAGTA CCAGGAGCTG GCTGAGCCTT CTTCACTAAC CTATGTCACT
    CGCTCAGAGA ACAGCATCTT TTTCGAGGTT GATGGGCCCT ACCTTGCCAT GATCCTTCCA
    GCCTCCAAGG AAGAAGGCAA GAAACTGAAG AAGAGGTATG CCGTGTTCAA TGAGGATGGT
    TCCCTGGCTG AGCTCAAGGG CTTTGAGGTC AAACGCCGTG GGGAGCTGCA GCTGATTAAA
    ATCTTCCAGT CCTCAGTGTT TGAGGCCTTT CTTAAGGGCA GCACCCTAGA AGAAGTGTAT
    GGCTCTGTCG CCAAGGTGGC TGATTACTGG CTGGACGTAC TATACAGCAA GGCAGCTAAC
    ATGCCTGATT CCGAACTGTT CGAGCTGATC TCCGAGAACC GTTCCATGTC TCGGAAGCTG
    GAAGATTATG GGGAGCAGAA GTCGACATCC ATCAGCACGG CCAAGCGCCT GGCTGAGTTC
    CTGGGAGATC AGATGGTGAA GGATGCAGGG CTGAGCTGCC GCTACATCAT CTCCCGGAAG
    CCTGAGGGGT CTCCAGTCAC AGAGAGAGCC ATTCCACTGG CCATTTTCCA GGCAGAGCCC
    ACCGTGAGGA AGCACTTTCT CCGAAAATGG CTAAAGAGTT CTTCCCTTCA GGACTTTGAT
    ATTCGGACAA TTCTGGATTG GGACTACTAC ATTGAGCGGC TGGGGAGTGC CATACAGAAG
    ATCATCACGA TCCCTGCGGC CCTGCAGCAG GTGAAGAACC CAGTACCGCG TGTCAAACAC
    CCTGACTGGC TGCACAAAAA ACTGTTAGAG AAAAATGACG TCTACAAGCA GAAGAAGATC
    AGTGAGCTCT TCGTCCTTGA GGGCAAGAGA CAGGTGGGCA TGGCCCAAGC TTCAGAAGGT
    TCCCAAAGCC TTGGCTCCCC CGACATGGAG GACTTTGGCC TCACAAAGCC ACCTCACTCG
    GCAGTGCCCA TTGCTACCAA GAGAAAGCGT ATCCACTGTG AGAGGCAAGA GGGATCGCAG
    GACCTGGAGC TGACGATGCC CTGGCAGGAG GTTTTGGGGC AGCCTCCGGC ACTCGGAACC
    ACCCAGGAAG AGTGGCTGGT CTGGCTCCAA TTCCACAAGA AGAAGTGGCA GCTGCAGGCT
    CAACAGCGCC TTGCTCACAA GAAGAGGCGG CGTCTGGAGG GGGCAGAGGG CACACCAAGG
    CCTGGGGCCA TCCGAGAGGG GCCCTCCACA GGACTGGGGG GCTTCTTTCG AAGAACAGCT
    CGCAGCATTC TGGACCTTCC ATGGCAGATT GTGCAGATCA GTGAGACCAG CCAGGCTGGC
    TTGTTCAGGG TATGGGCTGT CATCGGCAGT GACCTGCACT GCATCAAGCT GAACATTCCC
    CGGGTGTTCT ACGTGAACCA GCGGGTGGCC AAAGCGGAGG AGGGCCCTTC CTATCGGAAG
    GTGAATCGAG TCCTTCCTCG CTCCAATATG GTCTACAACC TCTACGAATA CTCGGTGCCA
    GAGGACATGT ACCAAGAACA TATCAACGAG ATCAACACTG AGCTGGCGGC GCCAGACATC
    GAGGGCGTGT ATGAGACTCA GGTTCCATTA GTGTTTCGGG CCCTGGTGCA GCTGGGCTGC
    ATGTGTGTGG TCAGTAAACA GCTGGTGAGG CACCTTTCAG GCTGGGAAGC AGAAACCTTC
    ACTCTTGAGC ACCTGGAGAT GCGTTCCTTG GCCCAGTTCA ACTACCTGGA ACCAGGCAGC
    ATCCGCCACA TCTACCTGTA CCACCACGCA CAGGGCCACA AGGCTCTCTT TGGCATCTTC
    GTACCCTCAC AGCGCAAGGC AGCTGTGTTT GTGCTGGATA CGGTGCGAAG CAACCAGATG
    CCCAGCCTCA GTGCCCTGTA CTCAACCGAG CACAGCCTCC TGCTGGAGAA AGTGGGCCCT
    GAGCTTCTGC CCCCACAGAA ACACGCTTTT GAAGTTCGTG CTGAAACAGA CTTGAAGATC
    GTCTGCAGAG CCATCCAGCG CTTCCTGTTG ACCTACAAAG AGGAGCGCCG TGGGCCAACT
    CTCATTGCTG TTCAGTCCAA CTGGGACCTG AAGAGGCTGA CTGGCGAGAT CCCTGTCCTG
    GAGGAATTCC CACTGGTGCC TGTCCGCGTG GCTGACAAGA TCAGCTACGG GGTCCTGGAC
    TGGCAGCGCC ACGGAGCCCG GCGCATGATC CGGCACTACC TCAACCTGGA CACGTATCTG
    TCACAGGCCT TCGAGATGAG CAGGTACTTC CACGTCCCCG TTGGGAATCT GCCTGAGGAC
    ATCTCTACCT TTGGCTCCGA CCTCTTCTTT GCCCGCCACC TCCAGCGCCA CAACCACCTG
    CTCTGGCTCT CTCCTACGTC TCGCCCAGAC CTGGGTGGGA AGGAGGCCGA CGACAACAGG
    CTCGTCATGG AGTTTGATGA CCAAGCCCCT GTGGAGATCA ACAGTTCCGG CTGTTACTCC
    ACAGTGTGCG TGGAACTGGA TATTCAGAAC CTGGCCGTCA ACACCATCCT GCAGTCTCAC
    CACGTCAATG ATATGGAGGG GGCCGACAGC ATTGGCATCA GCTTCGATGT GATCCAGCAG
    GCCTCCCTGG AGGACATGAT CACAGGCAAT CAGGCTGCCA GCGCCCCTGC CAGCTATGAC
    GAGACAGCCC TCTGCTCCAA CACCTTCAGG ATCCTGAAGA GCATGGTGGT GGGCTGGGTG
    AAGGAGATCA CGCAGTACCG CAACGTCTAC GCCGACAACC AGGTGATGCA CTTCTACCGC
    TGGCTCCGGT CGCCGTCCTC GCTGCTGCAC GACCCTGCCC TGCACCGCAC GCTGCACAGC
    ATGATGAAGA AGCTCTTCCT TCAACTCATC GCTGAGTTCA AGCGCCTGGG GTCATCGGTC
    GTCTATGCCA ACTTCAACCG CATCATCCTC TGCACCAAGA AGCGGCGGGT GGAGGACGCC
    CTGGCCTACG TGGAGTACAT CACCAACAGC ATCCACTCTA AAGAGATCTT CCATTCTTTG
    ACAATTTCCT TTTCTCGATG CTGGGAATTT CTTCTCTGGA TGGATCCCTC TAATTATGGT
    GGAATCAAAG GAACTGTTCC ATCTAGGATT CACTGTGGAC AGCAGGACTC CCCAGAAGAG
    GGCAGAGCAC AGCAGGGGGA ACCTGAGGAG GAGGAGGACG AGGACGAGGA GGAGGAGGAG
    GAGGAGGACG AGGGAGGCAC ACAGGAACTG GATGTCGAGG ACTTGCTGGA AAACAACTGG
    AACATCCTGC AGTTCCTGCC CCAGGCAGCC TCCTGCCAGA GCTACTTCCT CATGATCGTT
    TCAGCGTACA TCGTGGCCGT GTACCACAGC ATGAAGGAGG AACTGAGGCG CAGCACCCCA
    GGGAGCACCC CCGTGAGGAG GAGGGGAGCC AGCCAGCTCT CCCAGGAGGC CCAGGGGGAG
    GCCGGAGCCC TTCCTGGAAT GATCACCTTC TCACAAGATT ATGTGGCAAA TGAACTCACT
    CAGAACTTCT TCACCATCAC TCAGAAGATT CAGAAGAAAG TCACAGGCTC TCGGAACTCT
    ACTGAGCTCT CAGAAATGTT TCCTGTTCTC CCCGGTTCTC ACCTGCTGCT CAATAACCCT
    GCTCTGGAGT TCATCAAATA CGTGTGCAAG GTCCTGTCTC TGGACACCAA CATCACAAAC
    CAGGTGAACA AGCTAACCCG AGACCTGCTG CGCCTGGTAG ACGTAGGCGA GTTCTCAGAG
    GAGGCCCAGT TCCGAGACCC CTGCCGTTCC TACGTGCTCC CTGAGGTCAT CTGCCACAAC
    TGTAACTTCT GCCGGGACCT GGACCTGTGC AAAGACCCCT CCTTCTCTCA GGATGGGACC
    GTGCTGCCTC AGTGGCTCTG CTCCAACTGT CAGGTTGCCT ACGACTCATC AGTCATTGAG
    ATGGCCCTGG TGGAAGCCCT ACAGAAGAAG CTGATGGCCT TTACCCTGCA GGACCTGGTC
    TGCCTGAAGT GCCGGGGTGT GAAGGAGACC AACATGGCCG TGTACTGCAG CTGTGCGGGG
    GGCTTCTCCC TCACCATCCA CACTAAGGTC TTCATGGAAC AGATCGGAAT CTTCCAGAAC
    ATCGCCCAGC ACTATGGCAT GTCATACCTC AAGGAGACCC TGGATTGGCT GCAGCTTCAG
    AACCCCCAAG TGGGCCATTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa36974
    Clone ID Related Accession (Same CDS sequence) NM_001191429.1
    Accession Version NM_001191429.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6870bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product DNA polymerase epsilon catalytic subunit A
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02044945.1 and DAAA02044946.1. On Apr 21, 2011 this sequence version replaced XM_002701687.1. Sequence Note: This RefSeq record was created from genomic sequence data. The genomic coordinates used for the transcript record were predicted based on cow transcript alignments and on full-length homologous protein alignment with human NP_006222.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822095 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC 
    CGGGATGATG GCTCCTCTTC CTCTGTCTCG GCACTCAAGC GCCTGGAGCG CAGTCAGTGG
    ACAGATAAGA TGGATCTGCG GTTTGGTTTT GAGAGACTCA AGGAGCCTGG TGAGAAGACA
    GGCTGGCTCA TCAACATGCA CCCTACTGAG ATTTTAGATG AAGATAAACG CTTAGTTAGT
    GCTGTGGATT ACTACTTTAT TCAAGACGAT GGGAGCAGAT TTAAGGTGGC CTTGCCCTAC
    AAGCCATATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTT
    TCTAAGAAGT TTCAGGGTAA AATTGCCAAA GTAGAGACTG TCCCTAAGGA GGATCTGGAC
    TTGCCAAATC ACTTGGTTGG TTTGAAACGA AATTACATTA AGCTGTCCTT CAACACTGTG
    GAGGACCTCG TCAAAGTGAG GAAGGAGATC TCCCCAGCTG TGAAGAAAAA CCGGGACCAG
    AGTCATACCA GTGATGCCTA CACAACTATG CTCTCCAGTG TTCTGCAAGG GGGCAGTGTA
    ATTTTTGATG AAGAGGAAAC CTCTAAGAAG ATTGCCGACC AGTTGGACAA CATTGTGGAC
    ATGCGAGAGT ATGATGTACC TTACCACATC CGTCTCTCCA TCGACCTGAA GATCCACGTG
    GCTCACTGGT ACAATGTCCG GTACCGAGGA AGCGCCTTTC CAGTGGAAAT TGCCCGCCGT
    GATGACCTCG TTGAACGACC AGACCCCGTG GTTTTAGCAT TTGACATTGA GACAACCAAA
    CTGCCCCTCA AGTTTCCAGA TGCTGAGACC GACCAGATTA TGATGATTTC CTATATGATT
    GATGGTCAGG GCTACCTCAT CACCAACAGG GAGATTGTCT CAGAAGATAT AGAAGATTTC
    GAATTTACCC CCAAGCCAGA ATATGAAGGA CCCTTCTGTG TCTTCAATGA GCCCGATGAG
    GTTCATCTAA TCCAAAGATG GTTTGAACAC ATCCAGGAGG CCAAACCCAC CATCATGGTC
    ACCTATAATG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCAGAGCAGC GGTCCATGGA
    CTGAGCATGT ACCAGGAGAT AGGGTTCCAG AAGGACAGCC AGGGGGAGTA CAAATCACCC
    CAGTGTATCC ACATGGACTG TCTCAGGTGG GTGAAAAGGG ACAGTTACCT GCCCGTGGGC
    AGCCACAACC TCAAGGCGGC GGCCAAAGCC AAGCTGGGCT ACGACCCAGT GGAACTGGAC
    CCTGAAGACA TGTGCCGGAT GGCCACTGAG CAGCCCCAGA CTCTGGCGAC CTACTCGGTG
    TCGGATGCAG TGGCCACATA CTACCTGTAC ATGAAGTATG TCCACCCGTT TATATTCGCC
    CTGTGTACCA TCATCCCCAT GGAGCCCGAT GAGGTGCTGC GGAAAGGTTC TGGGACGCTG
    TGCGAGGCCT TGCTAATGGT GCAGGCCTTC CATGCCAACA TCATCTTCCC CAACAAGCAG
    GAGCAGGAGT TCAACAAGCT GACAGATGAC GGCCACGTGC TGGATGCTGA GACCTACGTG
    GGTGGCCACG TAGAAGCCCT GGAGTCCGGC GTGTTCCGCA GTGACATCCC CTGCCGGTTC
    CGGATGAATC CTGCTGCCTT TGACTTCCTG CTGCAGCGGG TGGAGAAAAC CATGCGTCAT
    GCCATCGAGG AAGAGGAGAA GGTGCCCATG GAGCAGGTCA TCAACTTCCA AGAGGTGTGT
    GATCAGATTA AGACCAAGCT CACCTCCCTG AAAGATGTTC CTAACCGAAT TGAGTGTCCC
    CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCTTGAC GAACCGCCTG
    CAGCCCTCTG CCATGGTGGA TGAGGCCACC TGCGCAGCCT GTGACTTCAA CAAGCCTGGA
    GCGAACTGCC AGAGGAAGAT GGCCTGGCAG TGGAGGGGAG AGTTCATGCC AGCCAGTCGC
    AGTGAGTATC ATCGGATCCA GCACCAACTG GAGTCAGAGA AGTTCCCTCC CTTGACCCCC
    GAGGGCCCAG CCCGGGCCTT TCACGAGCTG TCCCGTGAGG AGCAGGCTAA ATATGAGAAG
    CGGAGGCTGG CAGATTACTG CCGGAAGGCA TACAAGAAAA TCCACGTGAC CAAGGTGGAA
    GAGCGTCTCA CCACCATCTG CCAGCGGGAG AACTCCTTCT ATGTGGACAC TGTACGTGCT
    TTTCGGGACA GGCGCTATGA GTTCAAAGGT CTCCACAAGG TGTGGAAAAA GAAGCTCTCC
    GCAGCCGTGG AGGTGGGGGA CGCGGCGGAG GTGAAGCGCT GCAGGAACAT GGAGGTCCTG
    TACGACTCCC TGCAGCTGGC GCACAAGTGC ATCCTCAACT CCTTCTATGG CTACGTCATG
    CGCAAGGGGG CCCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC
    AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAAATCG GGAGGCCCTT AGAGCTGGAC
    ACAGATGGAA TATGGTGTGT CCTGCCCAAC AGCTTCCCCG AAAACTTTGT CATCAAGACC
    ACCAATGTGA AAAAGCCCAA GGTGACCATC TCTTACCCTG GGGCTATGTT AAACATCATG
    GTCAAGGAAG GCTTCACCAA TGATCAGTAC CAGGAGCTGG CTGAGCCTTC TTCACTAACC
    TATGTCACTC GCTCAGAGAA CAGCATCTTT TTCGAGGTTG ATGGGCCCTA CCTTGCCATG
    ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAACTGAAGA AGAGGTATGC CGTGTTCAAT
    GAGGATGGTT CCCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGTGG GGAGCTGCAG
    CTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTTC TTAAGGGCAG CACCCTAGAA
    GAAGTGTATG GCTCTGTCGC CAAGGTGGCT GATTACTGGC TGGACGTACT ATACAGCAAG
    GCAGCTAACA TGCCTGATTC CGAACTGTTC GAGCTGATCT CCGAGAACCG TTCCATGTCT
    CGGAAGCTGG AAGATTATGG GGAGCAGAAG TCGACATCCA TCAGCACGGC CAAGCGCCTG
    GCTGAGTTCC TGGGAGATCA GATGGTGAAG GATGCAGGGC TGAGCTGCCG CTACATCATC
    TCCCGGAAGC CTGAGGGGTC TCCAGTCACA GAGAGAGCCA TTCCACTGGC CATTTTCCAG
    GCAGAGCCCA CCGTGAGGAA GCACTTTCTC CGAAAATGGC TAAAGAGTTC TTCCCTTCAG
    GACTTTGATA TTCGGACAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGGAGTGCC
    ATACAGAAGA TCATCACGAT CCCTGCGGCC CTGCAGCAGG TGAAGAACCC AGTACCGCGT
    GTCAAACACC CTGACTGGCT GCACAAAAAA CTGTTAGAGA AAAATGACGT CTACAAGCAG
    AAGAAGATCA GTGAGCTCTT CGTCCTTGAG GGCAAGAGAC AGGTGGGCAT GGCCCAAGCT
    TCAGAAGGTT CCCAAAGCCT TGGCTCCCCC GACATGGAGG ACTTTGGCCT CACAAAGCCA
    CCTCACTCGG CAGTGCCCAT TGCTACCAAG AGAAAGCGTA TCCACTGTGA GAGGCAAGAG
    GGATCGCAGG ACCTGGAGCT GACGATGCCC TGGCAGGAGG TTTTGGGGCA GCCTCCGGCA
    CTCGGAACCA CCCAGGAAGA GTGGCTGGTC TGGCTCCAAT TCCACAAGAA GAAGTGGCAG
    CTGCAGGCTC AACAGCGCCT TGCTCACAAG AAGAGGCGGC GTCTGGAGGG GGCAGAGGGC
    ACACCAAGGC CTGGGGCCAT CCGAGAGGGG CCCTCCACAG GACTGGGGGG CTTCTTTCGA
    AGAACAGCTC GCAGCATTCT GGACCTTCCA TGGCAGATTG TGCAGATCAG TGAGACCAGC
    CAGGCTGGCT TGTTCAGGGT ATGGGCTGTC ATCGGCAGTG ACCTGCACTG CATCAAGCTG
    AACATTCCCC GGGTGTTCTA CGTGAACCAG CGGGTGGCCA AAGCGGAGGA GGGCCCTTCC
    TATCGGAAGG TGAATCGAGT CCTTCCTCGC TCCAATATGG TCTACAACCT CTACGAATAC
    TCGGTGCCAG AGGACATGTA CCAAGAACAT ATCAACGAGA TCAACACTGA GCTGGCGGCG
    CCAGACATCG AGGGCGTGTA TGAGACTCAG GTTCCATTAG TGTTTCGGGC CCTGGTGCAG
    CTGGGCTGCA TGTGTGTGGT CAGTAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA
    GAAACCTTCA CTCTTGAGCA CCTGGAGATG CGTTCCTTGG CCCAGTTCAA CTACCTGGAA
    CCAGGCAGCA TCCGCCACAT CTACCTGTAC CACCACGCAC AGGGCCACAA GGCTCTCTTT
    GGCATCTTCG TACCCTCACA GCGCAAGGCA GCTGTGTTTG TGCTGGATAC GGTGCGAAGC
    AACCAGATGC CCAGCCTCAG TGCCTTGTAC TCAACCGAGC ACAGCCTCCT GCTGGAGAAA
    GTGGGCCCTG AGCTTCTGCC CCCACAGAAA CACGCTTTTG AAGTTCGTGC TGAAACAGAC
    TTGAAGATCG TCTGCAGAGC CATCCAGCGC TTCCTGTTGA CCTACAAAGA GGAGCGCCGT
    GGGCCAACTC TCATTGCTGT TCAGTCCAAC TGGGACCTGA AGAGGCTGAC TGGCGAGATC
    CCTGTCCTGG AGGAATTCCC ACTGGTGCCT GTCCGCGTGG CTGACAAGAT CAGCTACGGG
    GTCCTGGACT GGCAGCGCCA CGGAGCCCGG CGCATGATCC GGCACTACCT CAACCTGGAC
    ACGTATCTGT CACAGGCCTT CGAGATGAGC AGGTACTTCC ACGTCCCCGT TGGGAATCTG
    CCTGAGGACA TCTCTACCTT TGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC
    AACCACCTGC TCTGGCTCTC TCCTACGTCT CGCCCAGACC TGGGTGGGAA GGAGGCCGAC
    GACAACAGGC TCGTCATGGA GTTTGATGAC CAAGCCCCTG TGGAGATCAA CAGTTCCGGC
    TGTTACTCCA CAGTGTGCGT GGAACTGGAT ATTCAGAACC TGGCCGTCAA CACCATCCTG
    CAGTCTCACC ACGTCAATGA TATGGAGGGG GCCGACAGCA TTGGCATCAG CTTCGATGTG
    ATCCAGCAGG CCTCCCTGGA GGACATGATC ACAGGCAATC AGGCTGCCAG CGCCCCTGCC
    AGCTATGACG AGACAGCCCT CTGCTCCAAC ACCTTCAGGA TCCTGAAGAG CATGGTGGTG
    GGCTGGGTGA AGGAGATCAC GCAGTACCGC AACGTCTACG CCGACAACCA GGTGATGCAC
    TTCTACCGCT GGCTCCGGTC GCCGTCCTCG CTGCTGCACG ACCCTGCCCT GCACCGCACG
    CTGCACAGCA TGATGAAGAA GCTCTTCCTT CAACTCATCG CTGAGTTCAA GCGCCTGGGG
    TCATCGGTCG TCTATGCCAA CTTCAACCGC ATCATCCTCT GCACCAAGAA GCGGCGGGTG
    GAGGACGCCC TGGCCTACGT GGAGTACATC ACCAACAGCA TCCACTCTAA AGAGATCTTC
    CATTCTTTGA CAATTTCCTT TTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCCTCT
    AATTATGGTG GAATCAAAGG AACTGTTCCA TCTAGGATTC ACTGTGGACA GCAGGACTCC
    CCAGAAGAGG GCAGAGCACA GCAGGGGGAA CCTGAGGAGG AGGAGGACGA GGACGAGGAG
    GAGGAGGAGG AGGAGGACGA GGGAGGCACA CAGGAACTGG ATGTCGAGGA CTTGCTGGAA
    AACAACTGGA ACATCCTGCA GTTCCTGCCC CAGGCAGCCT CCTGCCAGAG CTACTTCCTC
    ATGATCGTTT CAGCGTACAT CGTGGCCGTG TACCACAGCA TGAAGGAGGA ACTGAGGCGC
    AGCACCCCAG GGAGCACCCC CGTGAGGAGG AGGGGAGCCA GCCAGCTCTC CCAGGAGGCC
    CAGGGGGAGG CCGGAGCCCT TCCTGGAATG ATCACCTTCT CACAAGATTA TGTGGCAAAT
    GAACTCACTC AGAACTTCTT CACCATCACT CAGAAGATTC AGAAGAAAGT CACAGGCTCT
    CGGAACTCTA CTGAGCTCTC AGAAATGTTT CCTGTTCTCC CCGGTTCTCA CCTGCTGCTC
    AATAACCCTG CTCTGGAGTT CATCAAATAC GTGTGCAAGG TCCTGTCTCT GGACACCAAC
    ATCACAAACC AGGTGAACAA GCTAACCCGA GACCTGCTGC GCCTGGTAGA CGTAGGCGAG
    TTCTCAGAGG AGGCCCAGTT CCGAGACCCC TGCCGTTCCT ACGTGCTCCC TGAGGTCATC
    TGCCACAACT GTAACTTCTG CCGGGACCTG GACCTGTGCA AAGACCCCTC CTTCTCTCAG
    GATGGGACCG TGCTGCCTCA GTGGCTCTGC TCCAACTGTC AGGTTGCCTA CGACTCATCA
    GTCATTGAGA TGGCCCTGGT GGAAGCCCTA CAGAAGAAGC TGATGGCCTT TACCCTGCAG
    GACCTGGTCT GCCTGAAGTG CCGGGGTGTG AAGGAGACCA ACATGGCCGT GTACTGCAGC
    TGTGCGGGGG GCTTCTCCCT CACCATCCAC ACTAAGGTCT TCATGGAACA GATCGGAATC
    TTCCAGAACA TCGCCCAGCA CTATGGCATG TCATACCTCA AGGAGACCCT GGATTGGCTG
    CAGCTTCAGA ACCCCCAAGT GGGCCATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178358.1
    CDS1..6870
    Translation

    Target ORF information:

    RefSeq Version NM_001191429.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA polymerase epsilon, catalytic subunit (POLE), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191429.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC 
    CGGGATGATG GCTCCTCTTC CTCTGTCTCG GCACTCAAGC GCCTGGAGCG CAGTCAGTGG
    ACAGATAAGA TGGATCTGCG GTTTGGTTTT GAGAGACTCA AGGAGCCTGG TGAGAAGACA
    GGCTGGCTCA TCAACATGCA CCCTACTGAG ATTTTAGATG AAGATAAACG CTTAGTTAGT
    GCTGTGGATT ACTACTTTAT TCAAGACGAT GGGAGCAGAT TTAAGGTGGC CTTGCCCTAC
    AAGCCATATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTT
    TCTAAGAAGT TTCAGGGTAA AATTGCCAAA GTAGAGACTG TCCCTAAGGA GGATCTGGAC
    TTGCCAAATC ACTTGGTTGG TTTGAAACGA AATTACATTA AGCTGTCCTT CAACACTGTG
    GAGGACCTCG TCAAAGTGAG GAAGGAGATC TCCCCAGCTG TGAAGAAAAA CCGGGACCAG
    AGTCATACCA GTGATGCCTA CACAACTATG CTCTCCAGTG TTCTGCAAGG GGGCAGTGTA
    ATTTTTGATG AAGAGGAAAC CTCTAAGAAG ATTGCCGACC AGTTGGACAA CATTGTGGAC
    ATGCGAGAGT ATGATGTACC TTACCACATC CGTCTCTCCA TCGACCTGAA GATCCACGTG
    GCTCACTGGT ACAATGTCCG GTACCGAGGA AGCGCCTTTC CAGTGGAAAT TGCCCGCCGT
    GATGACCTCG TTGAACGACC AGACCCCGTG GTTTTAGCAT TTGACATTGA GACAACCAAA
    CTGCCCCTCA AGTTTCCAGA TGCTGAGACC GACCAGATTA TGATGATTTC CTATATGATT
    GATGGTCAGG GCTACCTCAT CACCAACAGG GAGATTGTCT CAGAAGATAT AGAAGATTTC
    GAATTTACCC CCAAGCCAGA ATATGAAGGA CCCTTCTGTG TCTTCAATGA GCCCGATGAG
    GTTCATCTAA TCCAAAGATG GTTTGAACAC ATCCAGGAGG CCAAACCCAC CATCATGGTC
    ACCTATAATG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCAGAGCAGC GGTCCATGGA
    CTGAGCATGT ACCAGGAGAT AGGGTTCCAG AAGGACAGCC AGGGGGAGTA CAAATCACCC
    CAGTGTATCC ACATGGACTG TCTCAGGTGG GTGAAAAGGG ACAGTTACCT GCCCGTGGGC
    AGCCACAACC TCAAGGCGGC GGCCAAAGCC AAGCTGGGCT ACGACCCAGT GGAACTGGAC
    CCTGAAGACA TGTGCCGGAT GGCCACTGAG CAGCCCCAGA CTCTGGCGAC CTACTCGGTG
    TCGGATGCAG TGGCCACATA CTACCTGTAC ATGAAGTATG TCCACCCGTT TATATTCGCC
    CTGTGTACCA TCATCCCCAT GGAGCCCGAT GAGGTGCTGC GGAAAGGTTC TGGGACGCTG
    TGCGAGGCCT TGCTAATGGT GCAGGCCTTC CATGCCAACA TCATCTTCCC CAACAAGCAG
    GAGCAGGAGT TCAACAAGCT GACAGATGAC GGCCACGTGC TGGATGCTGA GACCTACGTG
    GGTGGCCACG TAGAAGCCCT GGAGTCCGGC GTGTTCCGCA GTGACATCCC CTGCCGGTTC
    CGGATGAATC CTGCTGCCTT TGACTTCCTG CTGCAGCGGG TGGAGAAAAC CATGCGTCAT
    GCCATCGAGG AAGAGGAGAA GGTGCCCATG GAGCAGGTCA TCAACTTCCA AGAGGTGTGT
    GATCAGATTA AGACCAAGCT CACCTCCCTG AAAGATGTTC CTAACCGAAT TGAGTGTCCC
    CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCTTGAC GAACCGCCTG
    CAGCCCTCTG CCATGGTGGA TGAGGCCACC TGCGCAGCCT GTGACTTCAA CAAGCCTGGA
    GCGAACTGCC AGAGGAAGAT GGCCTGGCAG TGGAGGGGAG AGTTCATGCC AGCCAGTCGC
    AGTGAGTATC ATCGGATCCA GCACCAACTG GAGTCAGAGA AGTTCCCTCC CTTGACCCCC
    GAGGGCCCAG CCCGGGCCTT TCACGAGCTG TCCCGTGAGG AGCAGGCTAA ATATGAGAAG
    CGGAGGCTGG CAGATTACTG CCGGAAGGCA TACAAGAAAA TCCACGTGAC CAAGGTGGAA
    GAGCGTCTCA CCACCATCTG CCAGCGGGAG AACTCCTTCT ATGTGGACAC TGTACGTGCT
    TTTCGGGACA GGCGCTATGA GTTCAAAGGT CTCCACAAGG TGTGGAAAAA GAAGCTCTCC
    GCAGCCGTGG AGGTGGGGGA CGCGGCGGAG GTGAAGCGCT GCAGGAACAT GGAGGTCCTG
    TACGACTCCC TGCAGCTGGC GCACAAGTGC ATCCTCAACT CCTTCTATGG CTACGTCATG
    CGCAAGGGGG CCCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC
    AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAAATCG GGAGGCCCTT AGAGCTGGAC
    ACAGATGGAA TATGGTGTGT CCTGCCCAAC AGCTTCCCCG AAAACTTTGT CATCAAGACC
    ACCAATGTGA AAAAGCCCAA GGTGACCATC TCTTACCCTG GGGCTATGTT AAACATCATG
    GTCAAGGAAG GCTTCACCAA TGATCAGTAC CAGGAGCTGG CTGAGCCTTC TTCACTAACC
    TATGTCACTC GCTCAGAGAA CAGCATCTTT TTCGAGGTTG ATGGGCCCTA CCTTGCCATG
    ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAACTGAAGA AGAGGTATGC CGTGTTCAAT
    GAGGATGGTT CCCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGTGG GGAGCTGCAG
    CTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTTC TTAAGGGCAG CACCCTAGAA
    GAAGTGTATG GCTCTGTCGC CAAGGTGGCT GATTACTGGC TGGACGTACT ATACAGCAAG
    GCAGCTAACA TGCCTGATTC CGAACTGTTC GAGCTGATCT CCGAGAACCG TTCCATGTCT
    CGGAAGCTGG AAGATTATGG GGAGCAGAAG TCGACATCCA TCAGCACGGC CAAGCGCCTG
    GCTGAGTTCC TGGGAGATCA GATGGTGAAG GATGCAGGGC TGAGCTGCCG CTACATCATC
    TCCCGGAAGC CTGAGGGGTC TCCAGTCACA GAGAGAGCCA TTCCACTGGC CATTTTCCAG
    GCAGAGCCCA CCGTGAGGAA GCACTTTCTC CGAAAATGGC TAAAGAGTTC TTCCCTTCAG
    GACTTTGATA TTCGGACAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGGAGTGCC
    ATACAGAAGA TCATCACGAT CCCTGCGGCC CTGCAGCAGG TGAAGAACCC AGTACCGCGT
    GTCAAACACC CTGACTGGCT GCACAAAAAA CTGTTAGAGA AAAATGACGT CTACAAGCAG
    AAGAAGATCA GTGAGCTCTT CGTCCTTGAG GGCAAGAGAC AGGTGGGCAT GGCCCAAGCT
    TCAGAAGGTT CCCAAAGCCT TGGCTCCCCC GACATGGAGG ACTTTGGCCT CACAAAGCCA
    CCTCACTCGG CAGTGCCCAT TGCTACCAAG AGAAAGCGTA TCCACTGTGA GAGGCAAGAG
    GGATCGCAGG ACCTGGAGCT GACGATGCCC TGGCAGGAGG TTTTGGGGCA GCCTCCGGCA
    CTCGGAACCA CCCAGGAAGA GTGGCTGGTC TGGCTCCAAT TCCACAAGAA GAAGTGGCAG
    CTGCAGGCTC AACAGCGCCT TGCTCACAAG AAGAGGCGGC GTCTGGAGGG GGCAGAGGGC
    ACACCAAGGC CTGGGGCCAT CCGAGAGGGG CCCTCCACAG GACTGGGGGG CTTCTTTCGA
    AGAACAGCTC GCAGCATTCT GGACCTTCCA TGGCAGATTG TGCAGATCAG TGAGACCAGC
    CAGGCTGGCT TGTTCAGGGT ATGGGCTGTC ATCGGCAGTG ACCTGCACTG CATCAAGCTG
    AACATTCCCC GGGTGTTCTA CGTGAACCAG CGGGTGGCCA AAGCGGAGGA GGGCCCTTCC
    TATCGGAAGG TGAATCGAGT CCTTCCTCGC TCCAATATGG TCTACAACCT CTACGAATAC
    TCGGTGCCAG AGGACATGTA CCAAGAACAT ATCAACGAGA TCAACACTGA GCTGGCGGCG
    CCAGACATCG AGGGCGTGTA TGAGACTCAG GTTCCATTAG TGTTTCGGGC CCTGGTGCAG
    CTGGGCTGCA TGTGTGTGGT CAGTAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA
    GAAACCTTCA CTCTTGAGCA CCTGGAGATG CGTTCCTTGG CCCAGTTCAA CTACCTGGAA
    CCAGGCAGCA TCCGCCACAT CTACCTGTAC CACCACGCAC AGGGCCACAA GGCTCTCTTT
    GGCATCTTCG TACCCTCACA GCGCAAGGCA GCTGTGTTTG TGCTGGATAC GGTGCGAAGC
    AACCAGATGC CCAGCCTCAG TGCCTTGTAC TCAACCGAGC ACAGCCTCCT GCTGGAGAAA
    GTGGGCCCTG AGCTTCTGCC CCCACAGAAA CACGCTTTTG AAGTTCGTGC TGAAACAGAC
    TTGAAGATCG TCTGCAGAGC CATCCAGCGC TTCCTGTTGA CCTACAAAGA GGAGCGCCGT
    GGGCCAACTC TCATTGCTGT TCAGTCCAAC TGGGACCTGA AGAGGCTGAC TGGCGAGATC
    CCTGTCCTGG AGGAATTCCC ACTGGTGCCT GTCCGCGTGG CTGACAAGAT CAGCTACGGG
    GTCCTGGACT GGCAGCGCCA CGGAGCCCGG CGCATGATCC GGCACTACCT CAACCTGGAC
    ACGTATCTGT CACAGGCCTT CGAGATGAGC AGGTACTTCC ACGTCCCCGT TGGGAATCTG
    CCTGAGGACA TCTCTACCTT TGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC
    AACCACCTGC TCTGGCTCTC TCCTACGTCT CGCCCAGACC TGGGTGGGAA GGAGGCCGAC
    GACAACAGGC TCGTCATGGA GTTTGATGAC CAAGCCCCTG TGGAGATCAA CAGTTCCGGC
    TGTTACTCCA CAGTGTGCGT GGAACTGGAT ATTCAGAACC TGGCCGTCAA CACCATCCTG
    CAGTCTCACC ACGTCAATGA TATGGAGGGG GCCGACAGCA TTGGCATCAG CTTCGATGTG
    ATCCAGCAGG CCTCCCTGGA GGACATGATC ACAGGCAATC AGGCTGCCAG CGCCCCTGCC
    AGCTATGACG AGACAGCCCT CTGCTCCAAC ACCTTCAGGA TCCTGAAGAG CATGGTGGTG
    GGCTGGGTGA AGGAGATCAC GCAGTACCGC AACGTCTACG CCGACAACCA GGTGATGCAC
    TTCTACCGCT GGCTCCGGTC GCCGTCCTCG CTGCTGCACG ACCCTGCCCT GCACCGCACG
    CTGCACAGCA TGATGAAGAA GCTCTTCCTT CAACTCATCG CTGAGTTCAA GCGCCTGGGG
    TCATCGGTCG TCTATGCCAA CTTCAACCGC ATCATCCTCT GCACCAAGAA GCGGCGGGTG
    GAGGACGCCC TGGCCTACGT GGAGTACATC ACCAACAGCA TCCACTCTAA AGAGATCTTC
    CATTCTTTGA CAATTTCCTT TTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCCTCT
    AATTATGGTG GAATCAAAGG AACTGTTCCA TCTAGGATTC ACTGTGGACA GCAGGACTCC
    CCAGAAGAGG GCAGAGCACA GCAGGGGGAA CCTGAGGAGG AGGAGGACGA GGACGAGGAG
    GAGGAGGAGG AGGAGGACGA GGGAGGCACA CAGGAACTGG ATGTCGAGGA CTTGCTGGAA
    AACAACTGGA ACATCCTGCA GTTCCTGCCC CAGGCAGCCT CCTGCCAGAG CTACTTCCTC
    ATGATCGTTT CAGCGTACAT CGTGGCCGTG TACCACAGCA TGAAGGAGGA ACTGAGGCGC
    AGCACCCCAG GGAGCACCCC CGTGAGGAGG AGGGGAGCCA GCCAGCTCTC CCAGGAGGCC
    CAGGGGGAGG CCGGAGCCCT TCCTGGAATG ATCACCTTCT CACAAGATTA TGTGGCAAAT
    GAACTCACTC AGAACTTCTT CACCATCACT CAGAAGATTC AGAAGAAAGT CACAGGCTCT
    CGGAACTCTA CTGAGCTCTC AGAAATGTTT CCTGTTCTCC CCGGTTCTCA CCTGCTGCTC
    AATAACCCTG CTCTGGAGTT CATCAAATAC GTGTGCAAGG TCCTGTCTCT GGACACCAAC
    ATCACAAACC AGGTGAACAA GCTAACCCGA GACCTGCTGC GCCTGGTAGA CGTAGGCGAG
    TTCTCAGAGG AGGCCCAGTT CCGAGACCCC TGCCGTTCCT ACGTGCTCCC TGAGGTCATC
    TGCCACAACT GTAACTTCTG CCGGGACCTG GACCTGTGCA AAGACCCCTC CTTCTCTCAG
    GATGGGACCG TGCTGCCTCA GTGGCTCTGC TCCAACTGTC AGGTTGCCTA CGACTCATCA
    GTCATTGAGA TGGCCCTGGT GGAAGCCCTA CAGAAGAAGC TGATGGCCTT TACCCTGCAG
    GACCTGGTCT GCCTGAAGTG CCGGGGTGTG AAGGAGACCA ACATGGCCGT GTACTGCAGC
    TGTGCGGGGG GCTTCTCCCT CACCATCCAC ACTAAGGTCT TCATGGAACA GATCGGAATC
    TTCCAGAACA TCGCCCAGCA CTATGGCATG TCATACCTCA AGGAGACCCT GGATTGGCTG
    CAGCTTCAGA ACCCCCAAGT GGGCCATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27766
    Clone ID Related Accession (Same CDS sequence) XM_005217684.3
    Accession Version XM_005217684.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA polymerase epsilon catalytic subunit A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104452.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217684.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC 
    CGGGATGATG GCTCCTCTTC CTCTGTCTCG GCACTCAAGC GCCTGGAGCG CAGTCAGTGG
    ACAGATAAGA TGGATCTGCG GTTTGGTTTT GAGAGACTCA AGGAGCCTGG TGAGAAGACA
    GGCTGGCTCA TCAACATGCA CCCTACTGAG ATTTTAGATG AAGATAAACG CTTAGTTAGT
    GCTGTGGATT ACTACTTTAT TCAAGACGAT GGGAGCAGAT TTAAGGTGGC CTTGCCCTAC
    AAGCCATATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTT
    TCTAAGAAGT TTCAGGGTAA AATTGCCAAA GTAGAGACTG TCCCTAAGGA GGATCTGGAC
    TTGCCAAATC ACTTGGTTGG TTTGAAACGA AATTACATTA AGCTGTCCTT CAACACTGTG
    GAGGACCTCG TCAAAGTGAG GAAGGAGATC TCCCCAGCTG TGAAGAAAAA CCGGGACCAG
    AGTCATACCA GTGATGCCTA CACAACTATG CTCTCCAGTG TTCTGCAAGG GGGCAGTGTA
    ATTTTTGATG AAGAGGAAAC CTCTAAGAAG ATTGCCGACC AGTTGGACAA CATTGTGGAC
    ATGCGAGAGT ATGATGTACC TTACCACATC CGTCTCTCCA TCGACCTGAA GATCCACGTG
    GCTCACTGGT ACAATGTCCG GTACCGAGGA AGCGCCTTTC CAGTGGAAAT TGCCCGCCGT
    GATGACCTCG TTGAACGACC AGACCCCGTG GTTTTAGCAT TTGACATTGA GACAACCAAA
    CTGCCCCTCA AGTTTCCAGA TGCTGAGACC GACCAGATTA TGATGATTTC CTATATGATT
    GATGGTCAGG GCTACCTCAT CACCAACAGG GAGATTGTCT CAGAAGATAT AGAAGATTTC
    GAATTTACCC CCAAGCCAGA ATATGAAGGA CCCTTCTGTG TCTTCAATGA GCCCGATGAG
    GTTCATCTAA TCCAAAGATG GTTTGAACAC ATCCAGGAGG CCAAACCCAC CATCATGGTC
    ACCTATAATG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCAGAGCAGC GGTCCATGGA
    CTGAGCATGT ACCAGGAGAT AGGGTTCCAG AAGGACAGCC AGGGGGAGTA CAAATCACCC
    CAGTGTATCC ACATGGACTG TCTCAGGTGG GTGAAAAGGG ACAGTTACCT GCCCGTGGGC
    AGCCACAACC TCAAGGCGGC GGCCAAAGCC AAGCTGGGCT ACGACCCAGT GGAACTGGAC
    CCTGAAGACA TGTGCCGGAT GGCCACTGAG CAGCCCCAGA CTCTGGCGAC CTACTCGGTG
    TCGGATGCAG TGGCCACATA CTACCTGTAC ATGAAGTATG TCCACCCGTT TATATTCGCC
    CTGTGTACCA TCATCCCCAT GGAGCCCGAT GAGGTGCTGC GGAAAGGTTC TGGGACGCTG
    TGCGAGGCCT TGCTAATGGT GCAGGCCTTC CATGCCAACA TCATCTTCCC CAACAAGCAG
    GAGCAGGAGT TCAACAAGCT GACAGATGAC GGCCACGTGC TGGATGCTGA GACCTACGTG
    GGTGGCCACG TAGAAGCCCT GGAGTCCGGC GTGTTCCGCA GTGACATCCC CTGCCGGTTC
    CGGATGAATC CTGCTGCCTT TGACTTCCTG CTGCAGCGGG TGGAGAAAAC CATGCGTCAT
    GCCATCGAGG AAGAGGAGAA GGTGCCCATG GAGCAGGTCA TCAACTTCCA AGAGGTGTGT
    GATCAGATTA AGACCAAGCT CACCTCCCTG AAAGATGTTC CTAACCGAAT TGAGTGTCCC
    CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCTTGAC GAACCGCCTG
    CAGCCCTCTG CCATGGTGGA TGAGGCCACC TGCGCAGCCT GTGACTTCAA CAAGCCTGGA
    GCGAACTGCC AGAGGAAGAT GGCCTGGCAG TGGAGGGGAG AGTTCATGCC AGCCAGTCGC
    AGTGAGTATC ATCGGATCCA GCACCAACTG GAGTCAGAGA AGTTCCCTCC CTTGACCCCC
    GAGGGCCCAG CCCGGGCCTT TCACGAGCTG TCCCGTGAGG AGCAGGCTAA ATATGAGAAG
    CGGAGGCTGG CAGATTACTG CCGGAAGGCA TACAAGAAAA TCCACGTGAC CAAGGTGGAA
    GAGCGTCTCA CCACCATCTG CCAGCGGGAG AACTCCTTCT ATGTGGACAC TGTACGTGCT
    TTTCGGGACA GGCGCTATGA GTTCAAAGGT CTCCACAAGG TGTGGAAAAA GAAGCTCTCC
    GCAGCCGTGG AGGTGGGGGA CGCGGCGGAG GTGAAGCGCT GCAGGAACAT GGAGGTCCTG
    TACGACTCCC TGCAGCTGGC GCACAAGTGC ATCCTCAACT CCTTCTATGG CTACGTCATG
    CGCAAGGGGG CCCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC
    AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAAATCG GGAGGCCCTT AGAGCTGGAC
    ACAGATGGAA TATGGTGTGT CCTGCCCAAC AGCTTCCCCG AAAACTTTGT CATCAAGACC
    ACCAATGTGA AAAAGCCCAA GGTGACCATC TCTTACCCTG GGGCTATGTT AAACATCATG
    GTCAAGGAAG GCTTCACCAA TGATCAGTAC CAGGAGCTGG CTGAGCCTTC TTCACTAACC
    TATGTCACTC GCTCAGAGAA CAGCATCTTT TTCGAGGTTG ATGGGCCCTA CCTTGCCATG
    ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAACTGAAGA AGAGGTATGC CGTGTTCAAT
    GAGGATGGTT CCCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGTGG GGAGCTGCAG
    CTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTTC TTAAGGGCAG CACCCTAGAA
    GAAGTGTATG GCTCTGTCGC CAAGGTGGCT GATTACTGGC TGGACGTACT ATACAGCAAG
    GCAGCTAACA TGCCTGATTC CGAACTGTTC GAGCTGATCT CCGAGAACCG TTCCATGTCT
    CGGAAGCTGG AAGATTATGG GGAGCAGAAG TCGACATCCA TCAGCACGGC CAAGCGCCTG
    GCTGAGTTCC TGGGAGATCA GATGGTGAAG GATGCAGGGC TGAGCTGCCG CTACATCATC
    TCCCGGAAGC CTGAGGGGTC TCCAGTCACA GAGAGAGCCA TTCCACTGGC CATTTTCCAG
    GCAGAGCCCA CCGTGAGGAA GCACTTTCTC CGAAAATGGC TAAAGAGTTC TTCCCTTCAG
    GACTTTGATA TTCGGACAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGGAGTGCC
    ATACAGAAGA TCATCACGAT CCCTGCGGCC CTGCAGCAGG TGAAGAACCC AGTACCGCGT
    GTCAAACACC CTGACTGGCT GCACAAAAAA CTGTTAGAGA AAAATGACGT CTACAAGCAG
    AAGAAGATCA GTGAGCTCTT CGTCCTTGAG GGCAAGAGAC AGGTGGGCAT GGCCCAAGCT
    TCAGAAGGTT CCCAAAGCCT TGGCTCCCCC GACATGGAGG ACTTTGGCCT CACAAAGCCA
    CCTCACTCGG CAGTGCCCAT TGCTACCAAG AGAAAGCGTA TCCACTGTGA GAGGCAAGAG
    GGATCGCAGG ACCTGGAGCT GACGATGCCC TGGCAGGAGG TTTTGGGGCA GCCTCCGGCA
    CTCGGAACCA CCCAGGAAGA GTGGCTGGTC TGGCTCCAAT TCCACAAGAA GAAGTGGCAG
    CTGCAGGCTC AACAGCGCCT TGCTCACAAG AAGAGGCGGC GTCTGGAGGG GGCAGAGGGC
    ACACCAAGGC CTGGGGCCAT CCGAGAGGGG CCCTCCACAG GACTGGGGGG CTTCTTTCGA
    AGAACAGCTC GCAGCATTCT GGACCTTCCA TGGCAGATTG TGCAGATCAG TGAGACCAGC
    CAGGCTGGCT TGTTCAGGGT ATGGGCTGTC ATCGGCAGTG ACCTGCACTG CATCAAGCTG
    AACATTCCCC GGGTGTTCTA CGTGAACCAG CGGGTGGCCA AAGCGGAGGA GGGCCCTTCC
    TATCGGAAGG TGAATCGAGT CCTTCCTCGC TCCAATATGG TCTACAACCT CTACGAATAC
    TCGGTGCCAG AGGACATGTA CCAAGAACAT ATCAACGAGA TCAACACTGA GCTGGCGGCG
    CCAGACATCG AGGGCGTGTA TGAGACTCAG GTTCCATTAG TGTTTCGGGC CCTGGTGCAG
    CTGGGCTGCA TGTGTGTGGT CAGTAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA
    GAAACCTTCA CTCTTGAGCA CCTGGAGATG CGTTCCTTGG CCCAGTTCAA CTACCTGGAA
    CCAGGCAGCA TCCGCCACAT CTACCTGTAC CACCACGCAC AGGGCCACAA GGCTCTCTTT
    GGCATCTTCG TACCCTCACA GCGCAAGGCA GCTGTGTTTG TGCTGGATAC GGTGCGAAGC
    AACCAGATGC CCAGCCTCAG TGCCTTGTAC TCAACCGAGC ACAGCCTCCT GCTGGAGAAA
    GTGGGCCCTG AGCTTCTGCC CCCACAGAAA CACGCTTTTG AAGTTCGTGC TGAAACAGAC
    TTGAAGATCG TCTGCAGAGC CATCCAGCGC TTCCTGTTGA CCTACAAAGA GGAGCGCCGT
    GGGCCAACTC TCATTGCTGT TCAGTCCAAC TGGGACCTGA AGAGGCTGAC TGGCGAGATC
    CCTGTCCTGG AGGAATTCCC ACTGGTGCCT GTCCGCGTGG CTGACAAGAT CAGCTACGGG
    GTCCTGGACT GGCAGCGCCA CGGAGCCCGG CGCATGATCC GGCACTACCT CAACCTGGAC
    ACGTATCTGT CACAGGCCTT CGAGATGAGC AGGTACTTCC ACGTCCCCGT TGGGAATCTG
    CCTGAGGACA TCTCTACCTT TGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC
    AACCACCTGC TCTGGCTCTC TCCTACGTCT CGCCCAGACC TGGGTGGGAA GGAGGCCGAC
    GACAACAGGC TCGTCATGGA GTTTGATGAC CAAGCCCCTG TGGAGATCAA CAGTTCCGGC
    TGTTACTCCA CAGTGTGCGT GGAACTGGAT ATTCAGAACC TGGCCGTCAA CACCATCCTG
    CAGTCTCACC ACGTCAATGA TATGGAGGGG GCCGACAGCA TTGGCATCAG CTTCGATGTG
    ATCCAGCAGG CCTCCCTGGA GGACATGATC ACAGGCAATC AGGCTGCCAG CGCCCCTGCC
    AGCTATGACG AGACAGCCCT CTGCTCCAAC ACCTTCAGGA TCCTGAAGAG CATGGTGGTG
    GGCTGGGTGA AGGAGATCAC GCAGTACCGC AACGTCTACG CCGACAACCA GGTGATGCAC
    TTCTACCGCT GGCTCCGGTC GCCGTCCTCG CTGCTGCACG ACCCTGCCCT GCACCGCACG
    CTGCACAGCA TGATGAAGAA GCTCTTCCTT CAACTCATCG CTGAGTTCAA GCGCCTGGGG
    TCATCGGTCG TCTATGCCAA CTTCAACCGC ATCATCCTCT GCACCAAGAA GCGGCGGGTG
    GAGGACGCCC TGGCCTACGT GGAGTACATC ACCAACAGCA TCCACTCTAA AGAGATCTTC
    CATTCTTTGA CAATTTCCTT TTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCCTCT
    AATTATGGTG GAATCAAAGG AACTGTTCCA TCTAGGATTC ACTGTGGACA GGACTCCCCA
    GAAGAGGGCA GAGCACAGCA GGGGGAACCT GAGGAGGAGG AGGACGAGGA CGAGGAGGAG
    GAGGAGGAGG AGGACGAGGG AGGCACACAG GAACTGGATG TCGAGGACTT GCTGGAAAAC
    AACTGGAACA TCCTGCAGTT CCTGCCCCAG GCAGCCTCCT GCCAGAGCTA CTTCCTCATG
    ATCGTTTCAG CGTACATCGT GGCCGTGTAC CACAGCATGA AGGAGGAACT GAGGCGCAGC
    ACCCCAGGGA GCACCCCCGT GAGGAGGAGG GGAGCCAGCC AGCTCTCCCA GGAGGCCCAG
    GGGGAGGCCG GAGCCCTTCC TGGAATGATC ACCTTCTCAC AAGATTATGT GGCAAATGAA
    CTCACTCAGA ACTTCTTCAC CATCACTCAG AAGATTCAGA AGAAAGTCAC AGGCTCTCGG
    AACTCTACTG AGCTCTCAGA AATGTTTCCT GTTCTCCCCG GTTCTCACCT GCTGCTCAAT
    AACCCTGCTC TGGAGTTCAT CAAATACGTG TGCAAGGTCC TGTCTCTGGA CACCAACATC
    ACAAACCAGG TGAACAAGCT AACCCGAGAC CTGCTGCGCC TGGTAGACGT AGGCGAGTTC
    TCAGAGGAGG CCCAGTTCCG AGACCCCTGC CGTTCCTACG TGCTCCCTGA GGTCATCTGC
    CACAACTGTA ACTTCTGCCG GGACCTGGAC CTGTGCAAAG ACCCCTCCTT CTCTCAGGAT
    GGGACCGTGC TGCCTCAGTG GCTCTGCTCC AACTGTCAGG TTGCCTACGA CTCATCAGTC
    ATTGAGATGG CCCTGGTGGA AGCCCTACAG AAGAAGCTGA TGGCCTTTAC CCTGCAGGAC
    CTGGTCTGCC TGAAGTGCCG GGGTGTGAAG GAGACCAACA TGGCCGTGTA CTGCAGCTGT
    GCGGGGGGCT TCTCCCTCAC CATCCACACT AAGGTCTTCA TGGAACAGAT CGGAATCTTC
    CAGAACATCG CCCAGCACTA TGGCATGTCA TACCTCAAGG AGACCCTGGA TTGGCTGCAG
    CTTCAGAACC CCCAAGTGGG CCATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217741.1
    CDS54..6920
    Translation

    Target ORF information:

    RefSeq Version XM_005217684.3
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (DNA directed), epsilon, catalytic subunit (POLE), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217684.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC 
    CGGGATGATG GCTCCTCTTC CTCTGTCTCG GCACTCAAGC GCCTGGAGCG CAGTCAGTGG
    ACAGATAAGA TGGATCTGCG GTTTGGTTTT GAGAGACTCA AGGAGCCTGG TGAGAAGACA
    GGCTGGCTCA TCAACATGCA CCCTACTGAG ATTTTAGATG AAGATAAACG CTTAGTTAGT
    GCTGTGGATT ACTACTTTAT TCAAGACGAT GGGAGCAGAT TTAAGGTGGC CTTGCCCTAC
    AAGCCATATT TCTACATTGC GACCAGAAAG GGTTGTGAGC GAGAAGTTTC ATCTTTTCTT
    TCTAAGAAGT TTCAGGGTAA AATTGCCAAA GTAGAGACTG TCCCTAAGGA GGATCTGGAC
    TTGCCAAATC ACTTGGTTGG TTTGAAACGA AATTACATTA AGCTGTCCTT CAACACTGTG
    GAGGACCTCG TCAAAGTGAG GAAGGAGATC TCCCCAGCTG TGAAGAAAAA CCGGGACCAG
    AGTCATACCA GTGATGCCTA CACAACTATG CTCTCCAGTG TTCTGCAAGG GGGCAGTGTA
    ATTTTTGATG AAGAGGAAAC CTCTAAGAAG ATTGCCGACC AGTTGGACAA CATTGTGGAC
    ATGCGAGAGT ATGATGTACC TTACCACATC CGTCTCTCCA TCGACCTGAA GATCCACGTG
    GCTCACTGGT ACAATGTCCG GTACCGAGGA AGCGCCTTTC CAGTGGAAAT TGCCCGCCGT
    GATGACCTCG TTGAACGACC AGACCCCGTG GTTTTAGCAT TTGACATTGA GACAACCAAA
    CTGCCCCTCA AGTTTCCAGA TGCTGAGACC GACCAGATTA TGATGATTTC CTATATGATT
    GATGGTCAGG GCTACCTCAT CACCAACAGG GAGATTGTCT CAGAAGATAT AGAAGATTTC
    GAATTTACCC CCAAGCCAGA ATATGAAGGA CCCTTCTGTG TCTTCAATGA GCCCGATGAG
    GTTCATCTAA TCCAAAGATG GTTTGAACAC ATCCAGGAGG CCAAACCCAC CATCATGGTC
    ACCTATAATG GGGACTTTTT TGACTGGCCA TTTGTGGAGG CCAGAGCAGC GGTCCATGGA
    CTGAGCATGT ACCAGGAGAT AGGGTTCCAG AAGGACAGCC AGGGGGAGTA CAAATCACCC
    CAGTGTATCC ACATGGACTG TCTCAGGTGG GTGAAAAGGG ACAGTTACCT GCCCGTGGGC
    AGCCACAACC TCAAGGCGGC GGCCAAAGCC AAGCTGGGCT ACGACCCAGT GGAACTGGAC
    CCTGAAGACA TGTGCCGGAT GGCCACTGAG CAGCCCCAGA CTCTGGCGAC CTACTCGGTG
    TCGGATGCAG TGGCCACATA CTACCTGTAC ATGAAGTATG TCCACCCGTT TATATTCGCC
    CTGTGTACCA TCATCCCCAT GGAGCCCGAT GAGGTGCTGC GGAAAGGTTC TGGGACGCTG
    TGCGAGGCCT TGCTAATGGT GCAGGCCTTC CATGCCAACA TCATCTTCCC CAACAAGCAG
    GAGCAGGAGT TCAACAAGCT GACAGATGAC GGCCACGTGC TGGATGCTGA GACCTACGTG
    GGTGGCCACG TAGAAGCCCT GGAGTCCGGC GTGTTCCGCA GTGACATCCC CTGCCGGTTC
    CGGATGAATC CTGCTGCCTT TGACTTCCTG CTGCAGCGGG TGGAGAAAAC CATGCGTCAT
    GCCATCGAGG AAGAGGAGAA GGTGCCCATG GAGCAGGTCA TCAACTTCCA AGAGGTGTGT
    GATCAGATTA AGACCAAGCT CACCTCCCTG AAAGATGTTC CTAACCGAAT TGAGTGTCCC
    CTCATCTACC ACCTGGACGT GGGGGCCATG TACCCCAACA TCATCTTGAC GAACCGCCTG
    CAGCCCTCTG CCATGGTGGA TGAGGCCACC TGCGCAGCCT GTGACTTCAA CAAGCCTGGA
    GCGAACTGCC AGAGGAAGAT GGCCTGGCAG TGGAGGGGAG AGTTCATGCC AGCCAGTCGC
    AGTGAGTATC ATCGGATCCA GCACCAACTG GAGTCAGAGA AGTTCCCTCC CTTGACCCCC
    GAGGGCCCAG CCCGGGCCTT TCACGAGCTG TCCCGTGAGG AGCAGGCTAA ATATGAGAAG
    CGGAGGCTGG CAGATTACTG CCGGAAGGCA TACAAGAAAA TCCACGTGAC CAAGGTGGAA
    GAGCGTCTCA CCACCATCTG CCAGCGGGAG AACTCCTTCT ATGTGGACAC TGTACGTGCT
    TTTCGGGACA GGCGCTATGA GTTCAAAGGT CTCCACAAGG TGTGGAAAAA GAAGCTCTCC
    GCAGCCGTGG AGGTGGGGGA CGCGGCGGAG GTGAAGCGCT GCAGGAACAT GGAGGTCCTG
    TACGACTCCC TGCAGCTGGC GCACAAGTGC ATCCTCAACT CCTTCTATGG CTACGTCATG
    CGCAAGGGGG CCCGCTGGTA CTCCATGGAG ATGGCTGGCA TCGTCTGCTT CACAGGGGCC
    AACATCATCA CCCAGGCACG GGAGCTGATC GAGCAAATCG GGAGGCCCTT AGAGCTGGAC
    ACAGATGGAA TATGGTGTGT CCTGCCCAAC AGCTTCCCCG AAAACTTTGT CATCAAGACC
    ACCAATGTGA AAAAGCCCAA GGTGACCATC TCTTACCCTG GGGCTATGTT AAACATCATG
    GTCAAGGAAG GCTTCACCAA TGATCAGTAC CAGGAGCTGG CTGAGCCTTC TTCACTAACC
    TATGTCACTC GCTCAGAGAA CAGCATCTTT TTCGAGGTTG ATGGGCCCTA CCTTGCCATG
    ATCCTTCCAG CCTCCAAGGA AGAAGGCAAG AAACTGAAGA AGAGGTATGC CGTGTTCAAT
    GAGGATGGTT CCCTGGCTGA GCTCAAGGGC TTTGAGGTCA AACGCCGTGG GGAGCTGCAG
    CTGATTAAAA TCTTCCAGTC CTCAGTGTTT GAGGCCTTTC TTAAGGGCAG CACCCTAGAA
    GAAGTGTATG GCTCTGTCGC CAAGGTGGCT GATTACTGGC TGGACGTACT ATACAGCAAG
    GCAGCTAACA TGCCTGATTC CGAACTGTTC GAGCTGATCT CCGAGAACCG TTCCATGTCT
    CGGAAGCTGG AAGATTATGG GGAGCAGAAG TCGACATCCA TCAGCACGGC CAAGCGCCTG
    GCTGAGTTCC TGGGAGATCA GATGGTGAAG GATGCAGGGC TGAGCTGCCG CTACATCATC
    TCCCGGAAGC CTGAGGGGTC TCCAGTCACA GAGAGAGCCA TTCCACTGGC CATTTTCCAG
    GCAGAGCCCA CCGTGAGGAA GCACTTTCTC CGAAAATGGC TAAAGAGTTC TTCCCTTCAG
    GACTTTGATA TTCGGACAAT TCTGGATTGG GACTACTACA TTGAGCGGCT GGGGAGTGCC
    ATACAGAAGA TCATCACGAT CCCTGCGGCC CTGCAGCAGG TGAAGAACCC AGTACCGCGT
    GTCAAACACC CTGACTGGCT GCACAAAAAA CTGTTAGAGA AAAATGACGT CTACAAGCAG
    AAGAAGATCA GTGAGCTCTT CGTCCTTGAG GGCAAGAGAC AGGTGGGCAT GGCCCAAGCT
    TCAGAAGGTT CCCAAAGCCT TGGCTCCCCC GACATGGAGG ACTTTGGCCT CACAAAGCCA
    CCTCACTCGG CAGTGCCCAT TGCTACCAAG AGAAAGCGTA TCCACTGTGA GAGGCAAGAG
    GGATCGCAGG ACCTGGAGCT GACGATGCCC TGGCAGGAGG TTTTGGGGCA GCCTCCGGCA
    CTCGGAACCA CCCAGGAAGA GTGGCTGGTC TGGCTCCAAT TCCACAAGAA GAAGTGGCAG
    CTGCAGGCTC AACAGCGCCT TGCTCACAAG AAGAGGCGGC GTCTGGAGGG GGCAGAGGGC
    ACACCAAGGC CTGGGGCCAT CCGAGAGGGG CCCTCCACAG GACTGGGGGG CTTCTTTCGA
    AGAACAGCTC GCAGCATTCT GGACCTTCCA TGGCAGATTG TGCAGATCAG TGAGACCAGC
    CAGGCTGGCT TGTTCAGGGT ATGGGCTGTC ATCGGCAGTG ACCTGCACTG CATCAAGCTG
    AACATTCCCC GGGTGTTCTA CGTGAACCAG CGGGTGGCCA AAGCGGAGGA GGGCCCTTCC
    TATCGGAAGG TGAATCGAGT CCTTCCTCGC TCCAATATGG TCTACAACCT CTACGAATAC
    TCGGTGCCAG AGGACATGTA CCAAGAACAT ATCAACGAGA TCAACACTGA GCTGGCGGCG
    CCAGACATCG AGGGCGTGTA TGAGACTCAG GTTCCATTAG TGTTTCGGGC CCTGGTGCAG
    CTGGGCTGCA TGTGTGTGGT CAGTAAACAG CTGGTGAGGC ACCTTTCAGG CTGGGAAGCA
    GAAACCTTCA CTCTTGAGCA CCTGGAGATG CGTTCCTTGG CCCAGTTCAA CTACCTGGAA
    CCAGGCAGCA TCCGCCACAT CTACCTGTAC CACCACGCAC AGGGCCACAA GGCTCTCTTT
    GGCATCTTCG TACCCTCACA GCGCAAGGCA GCTGTGTTTG TGCTGGATAC GGTGCGAAGC
    AACCAGATGC CCAGCCTCAG TGCCTTGTAC TCAACCGAGC ACAGCCTCCT GCTGGAGAAA
    GTGGGCCCTG AGCTTCTGCC CCCACAGAAA CACGCTTTTG AAGTTCGTGC TGAAACAGAC
    TTGAAGATCG TCTGCAGAGC CATCCAGCGC TTCCTGTTGA CCTACAAAGA GGAGCGCCGT
    GGGCCAACTC TCATTGCTGT TCAGTCCAAC TGGGACCTGA AGAGGCTGAC TGGCGAGATC
    CCTGTCCTGG AGGAATTCCC ACTGGTGCCT GTCCGCGTGG CTGACAAGAT CAGCTACGGG
    GTCCTGGACT GGCAGCGCCA CGGAGCCCGG CGCATGATCC GGCACTACCT CAACCTGGAC
    ACGTATCTGT CACAGGCCTT CGAGATGAGC AGGTACTTCC ACGTCCCCGT TGGGAATCTG
    CCTGAGGACA TCTCTACCTT TGGCTCCGAC CTCTTCTTTG CCCGCCACCT CCAGCGCCAC
    AACCACCTGC TCTGGCTCTC TCCTACGTCT CGCCCAGACC TGGGTGGGAA GGAGGCCGAC
    GACAACAGGC TCGTCATGGA GTTTGATGAC CAAGCCCCTG TGGAGATCAA CAGTTCCGGC
    TGTTACTCCA CAGTGTGCGT GGAACTGGAT ATTCAGAACC TGGCCGTCAA CACCATCCTG
    CAGTCTCACC ACGTCAATGA TATGGAGGGG GCCGACAGCA TTGGCATCAG CTTCGATGTG
    ATCCAGCAGG CCTCCCTGGA GGACATGATC ACAGGCAATC AGGCTGCCAG CGCCCCTGCC
    AGCTATGACG AGACAGCCCT CTGCTCCAAC ACCTTCAGGA TCCTGAAGAG CATGGTGGTG
    GGCTGGGTGA AGGAGATCAC GCAGTACCGC AACGTCTACG CCGACAACCA GGTGATGCAC
    TTCTACCGCT GGCTCCGGTC GCCGTCCTCG CTGCTGCACG ACCCTGCCCT GCACCGCACG
    CTGCACAGCA TGATGAAGAA GCTCTTCCTT CAACTCATCG CTGAGTTCAA GCGCCTGGGG
    TCATCGGTCG TCTATGCCAA CTTCAACCGC ATCATCCTCT GCACCAAGAA GCGGCGGGTG
    GAGGACGCCC TGGCCTACGT GGAGTACATC ACCAACAGCA TCCACTCTAA AGAGATCTTC
    CATTCTTTGA CAATTTCCTT TTCTCGATGC TGGGAATTTC TTCTCTGGAT GGATCCCTCT
    AATTATGGTG GAATCAAAGG AACTGTTCCA TCTAGGATTC ACTGTGGACA GGACTCCCCA
    GAAGAGGGCA GAGCACAGCA GGGGGAACCT GAGGAGGAGG AGGACGAGGA CGAGGAGGAG
    GAGGAGGAGG AGGACGAGGG AGGCACACAG GAACTGGATG TCGAGGACTT GCTGGAAAAC
    AACTGGAACA TCCTGCAGTT CCTGCCCCAG GCAGCCTCCT GCCAGAGCTA CTTCCTCATG
    ATCGTTTCAG CGTACATCGT GGCCGTGTAC CACAGCATGA AGGAGGAACT GAGGCGCAGC
    ACCCCAGGGA GCACCCCCGT GAGGAGGAGG GGAGCCAGCC AGCTCTCCCA GGAGGCCCAG
    GGGGAGGCCG GAGCCCTTCC TGGAATGATC ACCTTCTCAC AAGATTATGT GGCAAATGAA
    CTCACTCAGA ACTTCTTCAC CATCACTCAG AAGATTCAGA AGAAAGTCAC AGGCTCTCGG
    AACTCTACTG AGCTCTCAGA AATGTTTCCT GTTCTCCCCG GTTCTCACCT GCTGCTCAAT
    AACCCTGCTC TGGAGTTCAT CAAATACGTG TGCAAGGTCC TGTCTCTGGA CACCAACATC
    ACAAACCAGG TGAACAAGCT AACCCGAGAC CTGCTGCGCC TGGTAGACGT AGGCGAGTTC
    TCAGAGGAGG CCCAGTTCCG AGACCCCTGC CGTTCCTACG TGCTCCCTGA GGTCATCTGC
    CACAACTGTA ACTTCTGCCG GGACCTGGAC CTGTGCAAAG ACCCCTCCTT CTCTCAGGAT
    GGGACCGTGC TGCCTCAGTG GCTCTGCTCC AACTGTCAGG TTGCCTACGA CTCATCAGTC
    ATTGAGATGG CCCTGGTGGA AGCCCTACAG AAGAAGCTGA TGGCCTTTAC CCTGCAGGAC
    CTGGTCTGCC TGAAGTGCCG GGGTGTGAAG GAGACCAACA TGGCCGTGTA CTGCAGCTGT
    GCGGGGGGCT TCTCCCTCAC CATCCACACT AAGGTCTTCA TGGAACAGAT CGGAATCTTC
    CAGAACATCG CCCAGCACTA TGGCATGTCA TACCTCAAGG AGACCCTGGA TTGGCTGCAG
    CTTCAGAACC CCCAAGTGGG CCATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.