gRNAs
The following POLE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OBa224428 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005217684.4 | |
| Accession Version | XM_005217684.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6867bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | DNA polymerase epsilon catalytic subunit A isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217684.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005217741.1 |
| CDS | 80..6946 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_005217684.4 |
1 | ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa224429 | |
| Clone ID Related Accession (Same CDS sequence) | XM_024977498.1 | |
| Accession Version | XM_024977498.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6741bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | DNA polymerase epsilon catalytic subunit A isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGATCTGC GGTTTGGTTT TGAGAGACTC AAGGAGCCTG GTGAGAAGAC AGGCTGGCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_024833266.1 |
| CDS | 153..6893 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_024977498.1 |
1 | ATGGATCTGC GGTTTGGTTT TGAGAGACTC AAGGAGCCTG GTGAGAAGAC AGGCTGGCTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa36974 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001191429.1 | |
| Accession Version | NM_001191429.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6870bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-22 | |
| Organism | Bos taurus(cattle) | |
| Product | DNA polymerase epsilon catalytic subunit A | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02044945.1 and DAAA02044946.1. On Apr 21, 2011 this sequence version replaced XM_002701687.1. Sequence Note: This RefSeq record was created from genomic sequence data. The genomic coordinates used for the transcript record were predicted based on cow transcript alignments and on full-length homologous protein alignment with human NP_006222.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822095 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001178358.1 |
| CDS | 1..6870 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001191429.1 |
1 | ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa27766 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005217684.3 | |
| Accession Version | XM_005217684.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6867bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-25 | |
| Organism | Bos taurus(cattle) | |
| Product | DNA polymerase epsilon catalytic subunit A isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104452.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217684.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005217741.1 |
| CDS | 54..6920 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005217684.3 |
1 | ATGGTTCTGA GGAATACCGG GCGACTGCGC GCGGAGCCGG GCGCGGACGG GGAGCCCAGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
A whole-genome assembly of the domestic cow, Bos taurus. |
