TIL2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIL2
Entrez Gene ID	817254
Full Name	DNA polymerase epsilon catalytic subunit
Synonyms	POL2B,T20P8.17,T20P8_17,TILTED 2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001336103.1	NP_001325160.1	DNA polymerase epsilon catalytic subunit
NM_001336102.1	NP_001325161.1	DNA polymerase epsilon catalytic subunit
NM_128270.3	NP_180280.2	DNA polymerase epsilon catalytic subunit

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	pole	NP_001121995.1
Arabidopsis thaliana (thale cress)	TIL2	NP_180280.2
Arabidopsis thaliana (thale cress)	TIL1	NP_172303.5
Oryza sativa (rice)	Os02g0511900	NP_001046939.2
Caenorhabditis elegans (roundworm)	F33H2.5	NP_493616.1
Canis lupus familiaris (dog)	POLE	XP_543348.3
Mus musculus (house mouse)	Pole	NP_035262.2
Kluyveromyces lactis	KLLA0C02585g	XP_452312.1
Neurospora crassa	NCU04548	XP_955939.2
Xenopus tropicalis (tropical clawed frog)	pole	XP_004910692.1
Drosophila melanogaster (fruit fly)	DNApol-epsilon	NP_524462.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004615	XP_315205.4
Saccharomyces cerevisiae (baker's yeast)	POL2	NP_014137.1
Eremothecium gossypii	AGOS_AFR657C	NP_986205.2
Magnaporthe oryzae (rice blast fungus)	MGG_03850	XP_003720041.1
Homo sapiens (human)	POLE	NP_006222.2
Pan troglodytes (chimpanzee)	POLE	XP_522564.4
Bos taurus (cattle)	POLE	NP_001178358.1
Rattus norvegicus (Norway rat)	Pole	NP_001100622.2
Gallus gallus (chicken)	POLE	XP_004934482.1
Schizosaccharomyces pombe (fission yeast)	cdc20	NP_596354.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicate that the role of Pol epsilon in replicative stress sensing is conserved in plants.
Title: Function of the Plant DNA Polymerase Epsilon in Replicative Stress Sensing, a Genetic Analysis.

role of DNA polymerase epsilon during gametophyte and seed development in A. thaliana, encoded by POL2a and POl2b, with a main regulatory subunit DPB2, is reported
Title: Genetic analysis of two Arabidopsis DNA polymerase epsilon subunits during early embryogenesis.

The following TIL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb36080	NM_001336102.1 Latest version!	Arabidopsis thaliana DNA polymerase epsilon catalytic subunit (TIL2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAb36081	NM_001336103.1 Latest version!	Arabidopsis thaliana DNA polymerase epsilon catalytic subunit (TIL2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OAb08111	NM_128270.3 Latest version!	Arabidopsis thaliana DNA polymerase epsilon catalytic subunit (TIL2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIL2 ( NM_001336102.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001325161.1 Arabidopsis thaliana DNA polymerase epsilon catalytic subunit (TIL2), mRNA.

Price & Availability↑

CloneID	OAb36080
Clone ID Related Accession (Same CDS sequence)	NM_001336102.1
Accession Version	NM_001336102.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	DNA polymerase epsilon catalytic subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071).

ATGAGCGGAA GGAGATGTGA TCGGAGGTTG AATGTGCAGA AAGTTTCAGC GGCAGATGAA 
CTCGAAACTA AACTTGGATT TGGGCTTTTC TCCCAAGGGG AAACGCGGCT CGGGTGGCTA 
CTCACGTTTG CATCATCATC TTGGGAAGAT GCCGATACTG GGAAAACCTT TAGTTGTGTG 
GACCTTTTCT TTGTCACTCA GGACGGCTCT TCTTTTAAGA CTAAGTACAA GTTCCGCCCT 
TACTTGTATG CCGCCACCAA AGACAATATG GAACTGGAAG TTGAAGCCTA CTTGAGACGC 
CGATATGAAA GACAAGTTGC TGATATTCAG ATTGTTCACA AAGAAGATCT TTATCTTAAA 
AATCATCTAT CTGGCCTGCA AAAAAAATAT CTCAAAGTAT CATTTGACAC TGTGCAACAA 
TTGGTGGAAG TAAAGAGGGA TCTGTTGCAT ATTGTCGAAC GAAACCTCGC AAAATTTAAT 
GCACTTGAAG CTTACGAATC CATCTTGTCT GGAAAACGAG AACAACGTCC TCAAGACTGC 
TTAGACTCTG TGGTGGACCT CCGCGAGTAC GATGTTCCTT ATCATGTCCG ATTTGCAATT 
GATAATGATG TGCGTAGTGG GCAATGGTAC AATGTCAGTA TTTCCAGCAC TGATGTCATA 
CTAGAGAAGA GGACGGATCT TCTCCAACGC GCAGAAGTTC GCGTTTGTGC ATTCGATATA 
GAGACAGTAA AGCTTCCATT GAAGTTCCCG GATGCTGAAT ATGATCAAAT TATGATGATA 
TCCTACATGG TTGATGGGCA AGGTTTTCTA ATTACTAACA GAGAGTGTGT TGGCAAAGAC 
ATAGAAGATC TGGAATATAC ACCCAAACCA GAGTTTGAAG GGTATTTCAA AGTCACGAAT 
GTTACCAACG AGGTTGAACT CCTCCGAAAA TGGTTTTCTC ATATGCAAGA GTTGAAACCT 
GGTATTTATG TTACATACAA CGGTGACTTT TTTGACTGGC CATTTATAGA ACGGAGAGCA 
TCTCATCATG GGATCAAAAT GAACGAGGAG TTGGGATTCC GTTGTGATCA AAATCAAGGT 
GAATGTCGAG CTAAGTTTGT GTGCCATTTG GATTGTTTTT CTTGGGTGAA ACGTGATAGC 
TATCTTCCTC AGGGAAGCCA GGGTCTGAAG GCTGTTACAA AGGTAAAACT GGGTTACGAT 
CCACTGGAAG TGAATCCTGA GGACATGGTT CGGTTTGCAA TGGAAAAGCC ACAGACAATG 
GCCTCCTACT CTGTCTCTGA TGCTGTTGCA ACTTACTACC TATACATGAC ATATGTCCAT 
CCGTTCGTTT TTTCTCTCGC AACTATCATT CCAATGGTCC CTGATGAGGT TTTACGCAAA 
GGGAGTGGCA CCCTTTGTGA AATGCTTTTG ATGGTTGAGG CTTACAAGGC AAATGTTGTA 
TGTCCCAACA AAAATCAAGC TGACCCTGAG AAGTTCTACC AAGGTAAACT TCTTGAAAGT 
GAGACATATA TTGGTGGCCA TGTAGAGTGC CTCCAAAGTG GTGTTTTCAG ATCTGACATT 
CCAACCAGTT TTAAGCTCGA CGCATCCGCA TACCAGCAAC TGATCGATAA CCTTGGTCGG 
GATCTGGAAT ATGCTATCAC TGTTGAAGGA AAAATGAGAA TGGATTCAGT TTCCAATTTT 
GATGAAGTAA AGGAAGTAAT CAGGGAAAAG CTCGAGAAGT TACGGGATGA TCCTATACGT 
GAAGAGGGGC CTCTTATTTA TCACCTTGAT GTCGCGGCGA TGTATCCAAA TATTATCTTA 
ACAAACAGGC TTCAGCCACC ATCAATAGTT ACGGATGAGG TTTGCACAGC ATGTGATTTC 
AACGGCCCGG AAAAGACCTG TCTTAGAAAA CTTGAATGGG TTTGGCGTGG GGTGACGTTC 
AAGGGAAATA AAAGTGAATA TTACCATCTG AAGAAGCAGA TTGAGTCTGA ATCTGTTGAT 
GCTGGAGCAA ATATGCAGTC TTCAAAACCT TTTCTTGATC TGCCAAAGGT GGAACAACAA 
TCCAAATTAA AAGAACGACT GAAAAAATAC TGTCAAAAGG CATATAGCCG AGTACTTGAC 
AAGCCAATCA CTGAAGTTCG CGAGGCTGGG ATATGCATGA GAGAAAACCC GTTCTATGTG 
GACACTGTGC GCAGCTTTCG AGATAGGAGG TATGAATACA AAACACTTAA CAAGGTCTGG 
AAGGGAAAGT TGTCAGAGGC CAAGGCAAGT GGCAATTTAA TCAAGATCCA GGAAGCACAT 
GACATGGTAG TGGTTTACGA TTCCTTGCAG CTAGCTCATA AGTGTATACT TAATTCCTTT 
TATGGATATG TCATGCGAAA GGGCGCAAGA TGGTACTCCA TGGAAATGGC TGGGGTAGTT 
ACTTATACTG GAGCCAAAAT CATCCAGAAT GCTCGTTTGC TTATTGAACG AATTGGGAAA 
CCACTTGAGC TGGATACAGA TGGTATATGG TGTGCTCTAC CTGGATCTTT TCCTGAGAAC 
TTTACCTTTA AAACCATAGA TATGAAGAAA TTCACCATCT CGTATCCGTG TGTAATACTT 
AATGTTGATG TGGCGAAGAA TAATTCAAAT GACCAATATC AGACTCTAGT AGATCCTGTA 
CGAAAGACAT ATAATTCACG TAGCGAATGC TCAATTGAAT TTGAAGTGGA TGGACCGTAC 
AAGGCAATGA TTATTCCTGC ATCCAAGGAA GAGGGAATCT TAATTAAGAA GCGGTATGCT 
GTTTTCAATC ATGACGGAAC CATAGCAGAA CTCAAGGGGT TTGAGATGAA GCGTAGAGGA 
GAGCTGAAAC TCATTAAAGT TTTCCAGGCC GAGCTATTTG ACAAATTTCT GCATGGATCA 
ACCCTTGAAG AGTGTTATTC TGCTGTTGCA GCTGTTGCAA ATCGTTGGCT TGACCTACTC 
GAGGGTCAAG GAAAAGATAT TGCCGATAGT GAATTGCTAG ATTATATATC AGAATCAAGC 
ACTATGAGCA AATCGTTAGC AGATTATGGT CAACAAAAGT CATGTGCAGT GACCACAGCA 
AAACGCCTGG CTGATTTTCT TGGTGATACA ATGGTCAAAG ATAAAGGACT GCGCTGCCAA 
TACATCGTTG CTCGTGAACC AGAGGGGACA CCTGTAAGTG AACGTGCTGT CCCTGTTGCC 
ATATTTCAAA CAGATGATCC TGAAAAAAAG TTTTATCTGC AAAAATGGTG CAAGATATCA 
TCATATACTG GAATCCGTTC AATTATTGAC TGGATGTATT ACAAGCAGCG TCTTCATTCA 
GCTATTCAAA AAGTTATTAC CATTCCTGCT GCAATGCAGA AGGTGGCAAA TCCTGTACTT 
AGGGTGCGTC ATCCTTATTG GCTCGAGAAA AAGGTCTGTG ACAAATTTCG TCAGGGAAAA 
ATAGTTGATA TGTTTAGCTC AGCAAACAAA GATCATTCGA CAACTCAAGA TAATGTAGTG 
GCGGATATTG AAGAGTTTTG CAAAGAAAAC AGACCTTCCG TAAAAGGGCC AAAGCCAGTT 
GCGCGTTCAT TTGAAGTCGA TAGAAATCAC TCTGAGGGTA AGCAGCAAGA GAGTTGGGAT 
CCAGAGTTCC ATGATATCTC ATTACAGAAC GTTGACAAGA ATGTAGATTA CCAAGGATGG 
CTTGAACTGG AAAAAAGAAA ATGGAAAATG ACTCTAACGA ATAAGAAAAA AAGAAGGTAC 
TCCTCAAGTT TATTTGGTTT TGATTTGGAA CAAAATATAA ATAAAAAAGT CTGTAAGGGA 
AGAGTAGGTG TAGGCTCATA CTTTAGGAGG CCTGAAGAAG CTTTGACTAG TTCCTATTTG 
CAGATAATAC AGTTGGTCCA AAGTCCACAG AGCGGGCAAT TTTTTGCTTG GGTTGTTGTG 
GAAGGATTGA TGCTTAAGAT CCCATTGACG ATCCCAAGGG TGTTTTACAT TAATTCCAAA 
GCTTCTATAG CTGGAAACTT CACGGGAAAG TGTATCAACA AGATTCTTCC TCACGGAAAG 
CCTTGTTACA ATTTGATGGA GGTCAATATT CAGGAAGATC AATTTATAAA AGAAAGCAAA 
AAGCTTGCCG CTCTACTTGC AGACCCCGAA ATTGAGGGCA TATATGAAAC TAAGATGCCT 
CTGGAGTTTA GTGCTATATG TCAGATTGGA TGTGTATGCA AAATTGAAGA CACGGCCAAA 
CACCGTAACA CCCAAGATGG ATGGAAACTT GGTGAACTTC ACAGGATAAC TACAACTGAG 
TGTCGCTACT TGGAGAATTC TATTCCACTT GTTTATTTGT ATCACAGCAC CTCAACAGGT 
CGTGCTGTTT ATGTTTTATA TTGTCATGCT TCGAAGCTTA TGTCTGTTGT GGTAGTCAAT 
CCTTACGGTG ACAAGGAATT ATTATCATCT GCCCTAGAGA GACAGTTTAG AGATAGATGC 
CAAGAACTGT CACCTGAGCC ATTTTCTTGG GATGGTATCC TTTTCCAGGT CGAGTATGTT 
GACCATCCAG AAGCTGCCAC GAAATTTTTA CAAAAAGCGC TCTGTGAATA CAGAGAAGAA 
AATTGTGGAG CAACTGTGGC AGTCATTGAA TGCCCTGACT TTAACACCAC GAAGGAAGGT 
GTAAAGGCCC TGGAAGATTT CCCATGTGTG AGGATTCCCT TCAACGACGA TGACAATAGT 
TATCAGCCTG TCTCCTGGCA ACGTCCAGCA GCAAAAATAG CAGTGCTCCG TTGTGCTTCA 
GCAATTCAGT GGTTGGACCG GAGGATTGCC CAGTCAAGAT ACGCCCATGT GCCTCTGGGG 
AATTTTGGAC GTGATTGGTT AACGTTCACA GTAGATATCT TCCTGTCCAG GGCACTCCGT 
GACCAGCAAC AGGTTCTTTG GGTCTCAGAT AATGGTGTTC CAGACCTTGG AGATATAAAC 
AATGAAGAGA CATTTCTAGC TGATGAGTTA CAAGAGACAA GTTTGCTATT CCCTGGAGCG 
TACAGAAAAG TGTCTGTGGA GTTAAAGGTC CATAGATTAG CTGTTAATGC CCTTCTGAAA 
AGTGACCTGG TGAGTGAAAT GGAAGGAGGC GGCTTTTTGG GTGTGAATTC TAGAGGAAGT 
AGCTTAAACG ATAATGGCAG TTTTGATGAG AATAATGGAT GCGCGCAAGC ATTTCGTGTC 
CTGAAACAAT TGATCAAGCG CTTGTTGCAT GATGCGTGTA ATTCTGGCAA TATATATGCT 
GATTCCATTT TGCAACATCT GTCTTGGTGG CTCCGCAGCC CTTCCTCAAA GCTTCACGAT 
CCAGCTCTCC ATCTCATGCT TCACAAGGTC ATGCAAAAGG TGTTTGCGCT GCTTTTGACT 
GATTTGCGGA GGTTAGGTGC TATAATAATA TACGCAGACT TCTCAAAGGT CATTATCGAC 
ACAGGGAAAT TTGATCTATC TGCTGCAAAG ACTTATTGTG AAAGCTTGCT CACAGTGATG 
GGGAGTAGAG ATATCTTTAA GTTGATCTTG CTTGAGCCGG TTCACTACTG GCACTCGTTA 
CTTTTCATGG ACCAGCATAA CTACGCTGGT ATCCGAGCTA CCGGAGATGA AATTTCAGGT 
AACGAAGTTA CTATTGAACC AAAGTGGAGC GTTGCACGGC ATTTACCCGA GTATATTCAG 
AAGGATTTTA TCATAATTGT TGCCACGTTT ATATTTGGCC CCTGGAAATT TGCATTAGAG 
AAAAAAAGGG GCAGTGCAGA GAGTTTAGAG GCAGAGATGG TAGAATATCT CAAGGAACAG 
ATAGGAACCC GGTTCATAAG CATGATTGTT GAGAAGATTG GTAATATCAG GTCACACATC 
AAGGATATAA ACGTGTCAGA TGCATCTTGG GCTTCTGGTC AAGCTCCCAA AGGAGATTAC 
ACGTTCGAAT TTATACAAAT TATTACTGCT GTTCTGGCTC TTGATCAAAA CGTTCAGCAA 
GATGTTCTGG TAATGAGAAA GATTCTGTTG AAGTACATTA AAGTAAAAGA ATGTGCTGCT 
GAAGCCGAAT TTATTGATCC TGGGCCATCC TTCATCTTAC CCAACGTGGC TTGCAGCAAC 
TGCGGTGCCT ACAGAGACCT AGATTTCTGC AGAGACTCAG CTCTCTTAAC AGAAAAAGAA 
TGGTCATGTG CGGATCCGCA ATGTGTGAAG ATATATGACA AAGAGCAGAT AGAGAGTAGC 
ATTATCCAGA TGGTGAGACA GAGGGAAAGA ATGTACCAGT TGCAGGATCT AGTGTGCAAT 
AGGTGCAATC AGGTGAAAGC CGCTCATTTG ACAGAGCAGT GTGAGTGTTC TGGATCCTTT 
AGATGCAAGG AGAGTGGTTC AGATTTCCAC AAGAGGATTG AGATTTTCTT GGATATAGCA 
AAGCGCCAGA AGTTTAGACT GCTAGAAGAA TGCATCTCCT GGATTTTATT TGCCACCAGC 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001325161.1
CDS	10..6435
Translation	MSGRRCDRRLNVQKVSAADELETKLGFGLFSQGETRLGWLLTFASSSWEDADTGKTFSCVDLFFVTQDGSSFKTKYKFRPYLYAATKDNMELEVEAYLRRRYERQVADIQIVHKEDLYLKNHLSGLQKKYLKVSFDTVQQLVEVKRDLLHIVERNLAKFNALEAYESILSGKREQRPQDCLDSVVDLREYDVPYHVRFAIDNDVRSGQWYNVSISSTDVILEKRTDLLQRAEVRVCAFDIETVKLPLKFPDAEYDQIMMISYMVDGQGFLITNRECVGKDIEDLEYTPKPEFEGYFKVTNVTNEVELLRKWFSHMQELKPGIYVTYNGDFFDWPFIERRASHHGIKMNEELGFRCDQNQGECRAKFVCHLDCFSWVKRDSYLPQGSQGLKAVTKVKLGYDPLEVNPEDMVRFAMEKPQTMASYSVSDAVATYYLYMTYVHPFVFSLATIIPMVPDEVLRKGSGTLCEMLLMVEAYKANVVCPNKNQADPEKFYQGKLLESETYIGGHVECLQSGVFRSDIPTSFKLDASAYQQLIDNLGRDLEYAITVEGKMRMDSVSNFDEVKEVIREKLEKLRDDPIREEGPLIYHLDVAAMYPNIILTNRLQPPSIVTDEVCTACDFNGPEKTCLRKLEWVWRGVTFKGNKSEYYHLKKQIESESVDAGANMQSSKPFLDLPKVEQQSKLKERLKKYCQKAYSRVLDKPITEVREAGICMRENPFYVDTVRSFRDRRYEYKTLNKVWKGKLSEAKASGNLIKIQEAHDMVVVYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIERIGKPLELDTDGIWCALPGSFPENFTFKTIDMKKFTISYPCVILNVDVAKNNSNDQYQTLVDPVRKTYNSRSECSIEFEVDGPYKAMIIPASKEEGILIKKRYAVFNHDGTIAELKGFEMKRRGELKLIKVFQAELFDKFLHGSTLEECYSAVAAVANRWLDLLEGQGKDIADSELLDYISESSTMSKSLADYGQQKSCAVTTAKRLADFLGDTMVKDKGLRCQYIVAREPEGTPVSERAVPVAIFQTDDPEKKFYLQKWCKISSYTGIRSIIDWMYYKQRLHSAIQKVITIPAAMQKVANPVLRVRHPYWLEKKVCDKFRQGKIVDMFSSANKDHSTTQDNVVADIEEFCKENRPSVKGPKPVARSFEVDRNHSEGKQQESWDPEFHDISLQNVDKNVDYQGWLELEKRKWKMTLTNKKKRRYSSSLFGFDLEQNINKKVCKGRVGVGSYFRRPEEALTSSYLQIIQLVQSPQSGQFFAWVVVEGLMLKIPLTIPRVFYINSKASIAGNFTGKCINKILPHGKPCYNLMEVNIQEDQFIKESKKLAALLADPEIEGIYETKMPLEFSAICQIGCVCKIEDTAKHRNTQDGWKLGELHRITTTECRYLENSIPLVYLYHSTSTGRAVYVLYCHASKLMSVVVVNPYGDKELLSSALERQFRDRCQELSPEPFSWDGILFQVEYVDHPEAATKFLQKALCEYREENCGATVAVIECPDFNTTKEGVKALEDFPCVRIPFNDDDNSYQPVSWQRPAAKIAVLRCASAIQWLDRRIAQSRYAHVPLGNFGRDWLTFTVDIFLSRALRDQQQVLWVSDNGVPDLGDINNEETFLADELQETSLLFPGAYRKVSVELKVHRLAVNALLKSDLVSEMEGGGFLGVNSRGSSLNDNGSFDENNGCAQAFRVLKQLIKRLLHDACNSGNIYADSILQHLSWWLRSPSSKLHDPALHLMLHKVMQKVFALLLTDLRRLGAIIIYADFSKVIIDTGKFDLSAAKTYCESLLTVMGSRDIFKLILLEPVHYWHSLLFMDQHNYAGIRATGDEISGNEVTIEPKWSVARHLPEYIQKDFIIIVATFIFGPWKFALEKKRGSAESLEAEMVEYLKEQIGTRFISMIVEKIGNIRSHIKDINVSDASWASGQAPKGDYTFEFIQIITAVLALDQNVQQDVLVMRKILLKYIKVKECAAEAEFIDPGPSFILPNVACSNCGAYRDLDFCRDSALLTEKEWSCADPQCVKIYDKEQIESSIIQMVRQRERMYQLQDLVCNRCNQVKAAHLTEQCECSGSFRCKESGSDFHKRIEIFLDIAKRQKFRLLEECISWILFATSC

Target ORF information:

RefSeq Version	NM_001336102.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana DNA polymerase epsilon catalytic subunit (TIL2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001336102.1

ORF Insert Sequence:

ATGAGCGGAA GGAGATGTGA TCGGAGGTTG AATGTGCAGA AAGTTTCAGC GGCAGATGAA 
CTCGAAACTA AACTTGGATT TGGGCTTTTC TCCCAAGGGG AAACGCGGCT CGGGTGGCTA 
CTCACGTTTG CATCATCATC TTGGGAAGAT GCCGATACTG GGAAAACCTT TAGTTGTGTG 
GACCTTTTCT TTGTCACTCA GGACGGCTCT TCTTTTAAGA CTAAGTACAA GTTCCGCCCT 
TACTTGTATG CCGCCACCAA AGACAATATG GAACTGGAAG TTGAAGCCTA CTTGAGACGC 
CGATATGAAA GACAAGTTGC TGATATTCAG ATTGTTCACA AAGAAGATCT TTATCTTAAA 
AATCATCTAT CTGGCCTGCA AAAAAAATAT CTCAAAGTAT CATTTGACAC TGTGCAACAA 
TTGGTGGAAG TAAAGAGGGA TCTGTTGCAT ATTGTCGAAC GAAACCTCGC AAAATTTAAT 
GCACTTGAAG CTTACGAATC CATCTTGTCT GGAAAACGAG AACAACGTCC TCAAGACTGC 
TTAGACTCTG TGGTGGACCT CCGCGAGTAC GATGTTCCTT ATCATGTCCG ATTTGCAATT 
GATAATGATG TGCGTAGTGG GCAATGGTAC AATGTCAGTA TTTCCAGCAC TGATGTCATA 
CTAGAGAAGA GGACGGATCT TCTCCAACGC GCAGAAGTTC GCGTTTGTGC ATTCGATATA 
GAGACAGTAA AGCTTCCATT GAAGTTCCCG GATGCTGAAT ATGATCAAAT TATGATGATA 
TCCTACATGG TTGATGGGCA AGGTTTTCTA ATTACTAACA GAGAGTGTGT TGGCAAAGAC 
ATAGAAGATC TGGAATATAC ACCCAAACCA GAGTTTGAAG GGTATTTCAA AGTCACGAAT 
GTTACCAACG AGGTTGAACT CCTCCGAAAA TGGTTTTCTC ATATGCAAGA GTTGAAACCT 
GGTATTTATG TTACATACAA CGGTGACTTT TTTGACTGGC CATTTATAGA ACGGAGAGCA 
TCTCATCATG GGATCAAAAT GAACGAGGAG TTGGGATTCC GTTGTGATCA AAATCAAGGT 
GAATGTCGAG CTAAGTTTGT GTGCCATTTG GATTGTTTTT CTTGGGTGAA ACGTGATAGC 
TATCTTCCTC AGGGAAGCCA GGGTCTGAAG GCTGTTACAA AGGTAAAACT GGGTTACGAT 
CCACTGGAAG TGAATCCTGA GGACATGGTT CGGTTTGCAA TGGAAAAGCC ACAGACAATG 
GCCTCCTACT CTGTCTCTGA TGCTGTTGCA ACTTACTACC TATACATGAC ATATGTCCAT 
CCGTTCGTTT TTTCTCTCGC AACTATCATT CCAATGGTCC CTGATGAGGT TTTACGCAAA 
GGGAGTGGCA CCCTTTGTGA AATGCTTTTG ATGGTTGAGG CTTACAAGGC AAATGTTGTA 
TGTCCCAACA AAAATCAAGC TGACCCTGAG AAGTTCTACC AAGGTAAACT TCTTGAAAGT 
GAGACATATA TTGGTGGCCA TGTAGAGTGC CTCCAAAGTG GTGTTTTCAG ATCTGACATT 
CCAACCAGTT TTAAGCTCGA CGCATCCGCA TACCAGCAAC TGATCGATAA CCTTGGTCGG 
GATCTGGAAT ATGCTATCAC TGTTGAAGGA AAAATGAGAA TGGATTCAGT TTCCAATTTT 
GATGAAGTAA AGGAAGTAAT CAGGGAAAAG CTCGAGAAGT TACGGGATGA TCCTATACGT 
GAAGAGGGGC CTCTTATTTA TCACCTTGAT GTCGCGGCGA TGTATCCAAA TATTATCTTA 
ACAAACAGGC TTCAGCCACC ATCAATAGTT ACGGATGAGG TTTGCACAGC ATGTGATTTC 
AACGGCCCGG AAAAGACCTG TCTTAGAAAA CTTGAATGGG TTTGGCGTGG GGTGACGTTC 
AAGGGAAATA AAAGTGAATA TTACCATCTG AAGAAGCAGA TTGAGTCTGA ATCTGTTGAT 
GCTGGAGCAA ATATGCAGTC TTCAAAACCT TTTCTTGATC TGCCAAAGGT GGAACAACAA 
TCCAAATTAA AAGAACGACT GAAAAAATAC TGTCAAAAGG CATATAGCCG AGTACTTGAC 
AAGCCAATCA CTGAAGTTCG CGAGGCTGGG ATATGCATGA GAGAAAACCC GTTCTATGTG 
GACACTGTGC GCAGCTTTCG AGATAGGAGG TATGAATACA AAACACTTAA CAAGGTCTGG 
AAGGGAAAGT TGTCAGAGGC CAAGGCAAGT GGCAATTTAA TCAAGATCCA GGAAGCACAT 
GACATGGTAG TGGTTTACGA TTCCTTGCAG CTAGCTCATA AGTGTATACT TAATTCCTTT 
TATGGATATG TCATGCGAAA GGGCGCAAGA TGGTACTCCA TGGAAATGGC TGGGGTAGTT 
ACTTATACTG GAGCCAAAAT CATCCAGAAT GCTCGTTTGC TTATTGAACG AATTGGGAAA 
CCACTTGAGC TGGATACAGA TGGTATATGG TGTGCTCTAC CTGGATCTTT TCCTGAGAAC 
TTTACCTTTA AAACCATAGA TATGAAGAAA TTCACCATCT CGTATCCGTG TGTAATACTT 
AATGTTGATG TGGCGAAGAA TAATTCAAAT GACCAATATC AGACTCTAGT AGATCCTGTA 
CGAAAGACAT ATAATTCACG TAGCGAATGC TCAATTGAAT TTGAAGTGGA TGGACCGTAC 
AAGGCAATGA TTATTCCTGC ATCCAAGGAA GAGGGAATCT TAATTAAGAA GCGGTATGCT 
GTTTTCAATC ATGACGGAAC CATAGCAGAA CTCAAGGGGT TTGAGATGAA GCGTAGAGGA 
GAGCTGAAAC TCATTAAAGT TTTCCAGGCC GAGCTATTTG ACAAATTTCT GCATGGATCA 
ACCCTTGAAG AGTGTTATTC TGCTGTTGCA GCTGTTGCAA ATCGTTGGCT TGACCTACTC 
GAGGGTCAAG GAAAAGATAT TGCCGATAGT GAATTGCTAG ATTATATATC AGAATCAAGC 
ACTATGAGCA AATCGTTAGC AGATTATGGT CAACAAAAGT CATGTGCAGT GACCACAGCA 
AAACGCCTGG CTGATTTTCT TGGTGATACA ATGGTCAAAG ATAAAGGACT GCGCTGCCAA 
TACATCGTTG CTCGTGAACC AGAGGGGACA CCTGTAAGTG AACGTGCTGT CCCTGTTGCC 
ATATTTCAAA CAGATGATCC TGAAAAAAAG TTTTATCTGC AAAAATGGTG CAAGATATCA 
TCATATACTG GAATCCGTTC AATTATTGAC TGGATGTATT ACAAGCAGCG TCTTCATTCA 
GCTATTCAAA AAGTTATTAC CATTCCTGCT GCAATGCAGA AGGTGGCAAA TCCTGTACTT 
AGGGTGCGTC ATCCTTATTG GCTCGAGAAA AAGGTCTGTG ACAAATTTCG TCAGGGAAAA 
ATAGTTGATA TGTTTAGCTC AGCAAACAAA GATCATTCGA CAACTCAAGA TAATGTAGTG 
GCGGATATTG AAGAGTTTTG CAAAGAAAAC AGACCTTCCG TAAAAGGGCC AAAGCCAGTT 
GCGCGTTCAT TTGAAGTCGA TAGAAATCAC TCTGAGGGTA AGCAGCAAGA GAGTTGGGAT 
CCAGAGTTCC ATGATATCTC ATTACAGAAC GTTGACAAGA ATGTAGATTA CCAAGGATGG 
CTTGAACTGG AAAAAAGAAA ATGGAAAATG ACTCTAACGA ATAAGAAAAA AAGAAGGTAC 
TCCTCAAGTT TATTTGGTTT TGATTTGGAA CAAAATATAA ATAAAAAAGT CTGTAAGGGA 
AGAGTAGGTG TAGGCTCATA CTTTAGGAGG CCTGAAGAAG CTTTGACTAG TTCCTATTTG 
CAGATAATAC AGTTGGTCCA AAGTCCACAG AGCGGGCAAT TTTTTGCTTG GGTTGTTGTG 
GAAGGATTGA TGCTTAAGAT CCCATTGACG ATCCCAAGGG TGTTTTACAT TAATTCCAAA 
GCTTCTATAG CTGGAAACTT CACGGGAAAG TGTATCAACA AGATTCTTCC TCACGGAAAG 
CCTTGTTACA ATTTGATGGA GGTCAATATT CAGGAAGATC AATTTATAAA AGAAAGCAAA 
AAGCTTGCCG CTCTACTTGC AGACCCCGAA ATTGAGGGCA TATATGAAAC TAAGATGCCT 
CTGGAGTTTA GTGCTATATG TCAGATTGGA TGTGTATGCA AAATTGAAGA CACGGCCAAA 
CACCGTAACA CCCAAGATGG ATGGAAACTT GGTGAACTTC ACAGGATAAC TACAACTGAG 
TGTCGCTACT TGGAGAATTC TATTCCACTT GTTTATTTGT ATCACAGCAC CTCAACAGGT 
CGTGCTGTTT ATGTTTTATA TTGTCATGCT TCGAAGCTTA TGTCTGTTGT GGTAGTCAAT 
CCTTACGGTG ACAAGGAATT ATTATCATCT GCCCTAGAGA GACAGTTTAG AGATAGATGC 
CAAGAACTGT CACCTGAGCC ATTTTCTTGG GATGGTATCC TTTTCCAGGT CGAGTATGTT 
GACCATCCAG AAGCTGCCAC GAAATTTTTA CAAAAAGCGC TCTGTGAATA CAGAGAAGAA 
AATTGTGGAG CAACTGTGGC AGTCATTGAA TGCCCTGACT TTAACACCAC GAAGGAAGGT 
GTAAAGGCCC TGGAAGATTT CCCATGTGTG AGGATTCCCT TCAACGACGA TGACAATAGT 
TATCAGCCTG TCTCCTGGCA ACGTCCAGCA GCAAAAATAG CAGTGCTCCG TTGTGCTTCA 
GCAATTCAGT GGTTGGACCG GAGGATTGCC CAGTCAAGAT ACGCCCATGT GCCTCTGGGG 
AATTTTGGAC GTGATTGGTT AACGTTCACA GTAGATATCT TCCTGTCCAG GGCACTCCGT 
GACCAGCAAC AGGTTCTTTG GGTCTCAGAT AATGGTGTTC CAGACCTTGG AGATATAAAC 
AATGAAGAGA CATTTCTAGC TGATGAGTTA CAAGAGACAA GTTTGCTATT CCCTGGAGCG 
TACAGAAAAG TGTCTGTGGA GTTAAAGGTC CATAGATTAG CTGTTAATGC CCTTCTGAAA 
AGTGACCTGG TGAGTGAAAT GGAAGGAGGC GGCTTTTTGG GTGTGAATTC TAGAGGAAGT 
AGCTTAAACG ATAATGGCAG TTTTGATGAG AATAATGGAT GCGCGCAAGC ATTTCGTGTC 
CTGAAACAAT TGATCAAGCG CTTGTTGCAT GATGCGTGTA ATTCTGGCAA TATATATGCT 
GATTCCATTT TGCAACATCT GTCTTGGTGG CTCCGCAGCC CTTCCTCAAA GCTTCACGAT 
CCAGCTCTCC ATCTCATGCT TCACAAGGTC ATGCAAAAGG TGTTTGCGCT GCTTTTGACT 
GATTTGCGGA GGTTAGGTGC TATAATAATA TACGCAGACT TCTCAAAGGT CATTATCGAC 
ACAGGGAAAT TTGATCTATC TGCTGCAAAG ACTTATTGTG AAAGCTTGCT CACAGTGATG 
GGGAGTAGAG ATATCTTTAA GTTGATCTTG CTTGAGCCGG TTCACTACTG GCACTCGTTA 
CTTTTCATGG ACCAGCATAA CTACGCTGGT ATCCGAGCTA CCGGAGATGA AATTTCAGGT 
AACGAAGTTA CTATTGAACC AAAGTGGAGC GTTGCACGGC ATTTACCCGA GTATATTCAG 
AAGGATTTTA TCATAATTGT TGCCACGTTT ATATTTGGCC CCTGGAAATT TGCATTAGAG 
AAAAAAAGGG GCAGTGCAGA GAGTTTAGAG GCAGAGATGG TAGAATATCT CAAGGAACAG 
ATAGGAACCC GGTTCATAAG CATGATTGTT GAGAAGATTG GTAATATCAG GTCACACATC 
AAGGATATAA ACGTGTCAGA TGCATCTTGG GCTTCTGGTC AAGCTCCCAA AGGAGATTAC 
ACGTTCGAAT TTATACAAAT TATTACTGCT GTTCTGGCTC TTGATCAAAA CGTTCAGCAA 
GATGTTCTGG TAATGAGAAA GATTCTGTTG AAGTACATTA AAGTAAAAGA ATGTGCTGCT 
GAAGCCGAAT TTATTGATCC TGGGCCATCC TTCATCTTAC CCAACGTGGC TTGCAGCAAC 
TGCGGTGCCT ACAGAGACCT AGATTTCTGC AGAGACTCAG CTCTCTTAAC AGAAAAAGAA 
TGGTCATGTG CGGATCCGCA ATGTGTGAAG ATATATGACA AAGAGCAGAT AGAGAGTAGC 
ATTATCCAGA TGGTGAGACA GAGGGAAAGA ATGTACCAGT TGCAGGATCT AGTGTGCAAT 
AGGTGCAATC AGGTGAAAGC CGCTCATTTG ACAGAGCAGT GTGAGTGTTC TGGATCCTTT 
AGATGCAAGG AGAGTGGTTC AGATTTCCAC AAGAGGATTG AGATTTTCTT GGATATAGCA 
AAGCGCCAGA AGTTTAGACT GCTAGAAGAA TGCATCTCCT GGATTTTATT TGCCACCAGC 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIL2 ( NM_001336103.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001325160.1 Arabidopsis thaliana DNA polymerase epsilon catalytic subunit (TIL2), partial mRNA.

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CloneID	OAb36081
Clone ID Related Accession (Same CDS sequence)	NM_001336103.1
Accession Version	NM_001336103.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	DNA polymerase epsilon catalytic subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). COMPLETENESS: incomplete on the 3' end.

ATGAGCGGAA GGAGATGTGA TCGGAGGTTG AATGTGCAGA AAGTTTCAGC GGCAGATGAA 
CTCGAAACTA AACTTGGATT TGGGCTTTTC TCCCAAGGGG AAACGCGGCT CGGGTGGCTA 
CTCACGTTTG CATCATCATC TTGGGAAGAT GCCGATACTG GGAAAACCTT TAGTTGTGTG 
GACCTTTTCT TTGTCACTCA GGACGGCTCT TCTTTTAAGA CTAAGTACAA GTTCCGCCCT 
TACTTGTATG CCGCCACCAA AGACAATATG GAACTGGAAG TTGAAGCCTA CTTGAGACGC 
CGATATGAAA GACAAGTTGC TGATATTCAG ATTGTTCACA AAGAAGATCT TTATCTTAAA 
AATCATCTAT CTGGCCTGCA AAAAAAATAT CTCAAAGTAT CATTTGACAC TGTGCAACAA 
TTGGTGGAAG TAAAGAGGGA TCTGTTGCAT ATTGTCGAAC GAAACCTCGC AAAATTTAAT 
GCACTTGAAG CTTACGAATC CATCTTGTCT GGAAAACGAG AACAACGTCC TCAAGACTGC 
TTAGACTCTG TGGTGGACCT CCGCGAGTAC GATGTTCCTT ATCATGTCCG ATTTGCAATT 
GATAATGATG TGCGTAGTGG GCAATGGTAC AATGTCAGTA TTTCCAGCAC TGATGTCATA 
CTAGAGAAGA GGACGGATCT TCTCCAACGC GCAGAAGTTC GCGTTTGTGC ATTCGATATA 
GAGACAGTAA AGCTTCCATT GAAGTTCCCG GATGCTGAAT ATGATCAAAT TATGATGATA 
TCCTACATGG TTGATGGGCA AGGTTTTCTA ATTACTAACA GAGAGTGTGT TGGCAAAGAC 
ATAGAAGATC TGGAATATAC ACCCAAACCA GAGTTTGAAG GGTATTTCAA AGTCACGAAT 
GTTACCAACG AGGTTGAACT CCTCCGAAAA TGGTTTTCTC ATATGCAAGA GTTGAAACCT 
GGTATTTATG TTACATACAA CGGTGACTTT TTTGACTGGC CATTTATAGA ACGGAGAGCA 
TCTCATCATG GGATCAAAAT GAACGAGGAG TTGGGATTCC GTTGTGATCA AAATCAAGGT 
GAATGTCGAG CTAAGTTTGT GTGCCATTTG GATTGTTTTT CTTGGGTGAA ACGTGATAGC 
TATCTTCCTC AGGGAAGCCA GGGTCTGAAG GCTGTTACAA AGGTAAAACT GGGTTACGAT 
CCACTGGAAG TGAATCCTGA GGACATGGTT CGGTTTGCAA TGGAAAAGCC ACAGACAATG 
GCCTCCTACT CTGTCTCTGA TGCTGTTGCA ACTTACTACC TATACATGAC ATATGTCCAT 
CCGTTCGTTT TTTCTCTCGC AACTATCATT CCAATGGTCC CTGATGAGGT TTTACGCAAA 
GGGAGTGGCA CCCTTTGTGA AATGCTTTTG ATGGTTGAGG CTTACAAGGC AAATGTTGTA 
TGTCCCAACA AAAATCAAGC TGACCCTGAG AAGTTCTACC AAGGTAAACT TCTTGAAAGT 
GAGACATATA TTGGTGGCCA TGTAGAGTGC CTCCAAAGTG GTGTTTTCAG ATCTGACATT 
CCAACCAGTT TTAAGCTCGA CGCATCCGCA TACCAGCAAC TGATCGATAA CCTTGGTCGG 
GATCTGGAAT ATGCTATCAC TGTTGAAGGA AAAATGAGAA TGGATTCAGT TTCCAATTTT 
GATGAAGTAA AGGAAGTAAT CAGGGAAAAG CTCGAGAAGT TACGGGATGA TCCTATACGT 
GAAGAGGGGC CTCTTATTTA TCACCTTGAT GTCGCGGCGA TGTATCCAAA TATTATCTTA 
ACAAACAGGC TTCAGCCACC ATCAATAGTT ACGGATGAGG TTTGCACAGC ATGTGATTTC 
AACGGCCCGG AAAAGACCTG TCTTAGAAAA CTTGAATGGG TTTGGCGTGG GGTGACGTTC 
AAGGGAAATA AAAGTGAATA TTACCATCTG AAGAAGCAGA TTGAGTCTGA ATCTGTTGAT 
GCTGGAGCAA ATATGCAGTC TTCAAAACCT TTTCTTGATC TGCCAAAGGT GGAACAACAA 
TCCAAATTAA AAGAACGACT GAAAAAATAC TGTCAAAAGG CATATAGCCG AGTACTTGAC 
AAGCCAATCA CTGAAGTTCG CGAGGCTGGG ATATGCATGA GAGAAAACCC GTTCTATGTG 
GACACTGTGC GCAGCTTTCG AGATAGGAGG TATGAATACA AAACACTTAA CAAGGTCTGG 
AAGGGAAAGT TGTCAGAGGC CAAGGCAAGT GGCAATTTAA TCAAGATCCA GGAAGCACAT 
GACATGGTAG TGGTTTACGA TTCCTTGCAG CTAGCTCATA AGTGTATACT TAATTCCTTT 
TATGGATATG TCATGCGAAA GGGCGCAAGA TGGTACTCCA TGGAAATGGC TGGGGTAGTT 
ACTTATACTG GAGCCAAAAT CATCCAGAAT GCTCGTTTGC TTATTGAACG AATTGGGAAA 
CCACTTGAGC TGGATACAGA TGGTATATGG TGTGCTCTAC CTGGATCTTT TCCTGAGAAC 
TTTACCTTTA AAACCATAGA TATGAAGAAA TTCACCATCT CGTATCCGTG TGTAATACTT 
AATGTTGATG TGGCGAAGAA TAATTCAAAT GACCAATATC AGACTCTAGT AGATCCTGTA 
CGAAAGACAT ATAATTCACG TAGCGAATGC TCAATTGAAT TTGAAGTGGA TGGACCGTAC 
AAGGCAATGA TTATTCCTGC ATCCAAGGAA GAGGGAATCT TAATTAAGAA GCGGTATGCT 
GTTTTCAATC ATGACGGAAC CATAGCAGAA CTCAAGGGGT TTGAGATGAA GCGTAGAGGA 
GAGCTGAAAC TCATTAAAGT TTTCCAGGCC GAGCTATTTG ACAAATTTCT GCATGGATCA 
ACCCTTGAAG AGTGTTATTC TGCTGTTGCA GCTGTTGCAA ATCGTTGGCT TGACCTACTC 
GAGGGTCAAG GAAAAGATAT TGCCGATAGT GAATTGCTAG ATTATATATC AGAATCAAGC 
ACTATGAGCA AATCGTTAGC AGATTATGGT CAACAAAAGT CATGTGCAGT GACCACAGCA 
AAACGCCTGG CTGATTTTCT TGGTGATACA ATGGTCAAAG ATAAAGGACT GCGCTGCCAA 
TACATCGTTG CTCGTGAACC AGAGGGGACA CCTGTAAGTG AACGTGCTGT CCCTGTTGCC 
ATATTTCAAA CAGATGATCC TGAAAAAAAG TTTTATCTGC AAAAATGGTG CAAGATATCA 
TCATATACTG GAATCCGTTC AATTATTGAC TGGATGTATT ACAAGCAGCG TCTTCATTCA 
GCTATTCAAA AAGTTATTAC CATTCCTGCT GCAATGCAGA AGGTGGCAAA TCCTGTACTT 
AGGGTGCGTC ATCCTTATTG GCTCGAGAAA AAGGTCTGTG ACAAATTTCG TCAGGGAAAA 
ATAGTTGATA TGTTTAGCTC AGCAAACAAA GATCATTCGA CAACTCAAGA TAATGTAGTG 
GCGGATATTG AAGAGTTTTG CAAAGAAAAC AGACCTTCCG TAAAAGGGCC AAAGCCAGTT 
GCGCGTTCAT TTGAAGTCGA TAGAAATCAC TCTGAGGGTA AGCAGCAAGA GAGTTGGGAT 
CCAGAGTTCC ATGATATCTC ATTACAGAAC GTTGACAAGA ATGTAGATTA CCAAGGATGG 
CTTGAACTGG AAAAAAGAAA ATGGAAAATG ACTCTAACGA ATAAGAAAAA AAGAAGGTTT 
GATGATCTAA AGCCTTGTAA TCAGATTGAT GCACACAAGA TAAATAAAAA AGTCTGTAAG 
GGAAGAGTAG GTGTAGGCTC ATACTTTAGG AGGCCTGAAG AAGCTTTGAC TAGTTCCTAT 
TTGCAGATAA TACAGTTGGT CCAAAGTCCA CAGAGCGGGC AATTTTTTGC TTGGGTTGTT 
GTGGAAGGAT TGATGCTTAA GATCCCATTG ACGATCCCAA GGGTGTTTTA CATTAATTCC 
AAAGCTTCTA TAGCTGGAAA CTTCACGGGA AAGTGTATCA ACAAGATTCT TCCTCACGGA 
AAGCCTTGTT ACAATTTGAT GGAGGTCAAT ATTCAGGAAG ATCAATTTAT AAAAGAAAGC 
AAAAAGCTTG CCGCTCTACT TGCAGACCCC GAAATTGAGG GCATATATGA AACTAAGATG 
CCTCTGGAGT TTAGTGCTAT ATGTCAGATT GGATGTGTAT GCAAAATTGA AGACACGGCC 
AAACACCGTA ACACCCAAGA TGGATGGAAA CTTGGTGAAC TTCACAGGAT AACTACAACT 
GAGTGTCGCT ACTTGGAGAA TTCTATTCCA CTTGTTTATT TGTATCACAG CACCTCAACA 
GGTCGTGCTG TTTATGTTTT ATATTGTCAT GCTTCGAAGC TTATGTCTGT TGTGGTAGTC 
AATCCTTACG GTGACAAGGA ATTATTATCA TCTGCCCTAG AGAGACAGTT TAGAGATAGA 
TGCCAAGAAC TGTCACCTGA GCCATTTTCT TGGGATGGTA TCCTTTTCCA GGTCGAGTAT 
GTTGACCATC CAGAAGCTGC CACGAAATTT TTACAAAAAG CGCTCTGTGA ATACAGAGAA 
GAAAATTGTG GAGCAACTGT GGCAGTCATT GAATGCCCTG ACTTTAACAC CACGAAGGAA 
GGTGTAAAGG CCCTGGAAGA TTTCCCATGT GTGAGGATTC CCTTCAACGA CGATGACAAT 
AGTTATCAGC CTGTCTCCTG GCAACGTCCA GCAGCAAAAA TAGCAGTGCT CCGTTGTGCT 
TCAGCAATTC AGTGGTTGGA CCGGAGGATT GCCCAGTCAA GATACGCCCA TGTGCCTCTG 
GGGAATTTTG GACGTGATTG GTTAACGTTC ACAGTAGATA TCTTCCTGTC CAGGGCACTC 
CGTGACCAGC AACAGGTTCT TTGGGTCTCA GATAATGGTG TTCCAGACCT TGGAGATATA 
AACAATGAAG AGACATTTCT AGCTGATGAG ACAAGTTTGC TATTCCCTGG AGCGTACAGA 
AAAGTGTCTG TGGAGTTAAA GGTCCATAGA TTAGCTGTTA ATGCCCTTCT GAAAAGTGAC 
CTGGTGAGTG AAATGGAAGG AGGCGGCTTT TTGGGTGTGA ATTCTAGAGG AAGTAGCTTA 
AACGATAATG GCAGTTTTGA TGAGAATAAT GGATGCGCGC AAGCATTTCG TGTCCTGAAA 
CAATTGATCA AGCGCTTGTT GCATGATGCG TGTAATTCTG GCAATATATA TGCTGATTCC 
ATTTTGCAAC ATCTGTCTTG GTGGCTCCGC AGCCCTTCCT CAAAGCTTCA CGATCCAGCT 
CTCCATCTCA TGCTTCACAA GGTCATGCAA AAGGTGTTTG CGCTGCTTTT GACTGATTTG 
CGGAGGTTAG GTGCTATAAT AATATACGCA GACTTCTCAA AGGTCATTAT CGACACAGGG 
AAATTTGATC TATCTGCTGC AAAGACTTAT TGTGAAAGCT TGCTCACAGT GATGGGGAGT 
AGAGATATCT TTAAGTTGAT CTTGCTTGAG CCGGTTCACT ACTGGCACTC GTTACTTTTC 
ATGGACCAGC ATAACTACGC TGGTATCCGA GCTACCGGAG ATGAAATTTC AGGTAACGAA 
GTTACTATTG AACCAAAGTG GAGCGTTGCA CGGCATTTAC CCGAGTATAT TCAGAAGGAT 
TTTATCATAA TTGTTGCCAC GTTTATATTT GGCCCCTGGA AATTTGCATT AGAGAAAAAA 
AGGGGCAGTG CAGAGAGTTT AGAGGCAGAG ATGGTAGAAT ATCTCAAGGA ACAGATAGGA 
ACCCGGTTCA TAAGCATGAT TGTTGAGAAG ATTGGTAATA TCAGGTCACA CATCAAGGAT 
ATAAACGTGT CAGATGCATC TTGGGCTTCT GGTCAAGCTC CCAAAGGAGA TTACACGTTC 
GAATTTATAC AAATTATTAC TGCTGTTCTG GCTCTTGATC AAAACGTTCA GCAAGATGTT 
CTGGTAATGA GAAAGATTCT GTTGAAGTAC ATTAAAGTAA AAGAATGTGC TGCTGAAGCC 
GAATTTATTG ATCCTGGGCC ATCCTTCATC TTACCCAACG TGGCTTGCAG CAACTGCGGT 
GCCTACAGAG ACCTAGATTT CTGCAGAGAC TCAGCTCTCT TAACAGAAAA AGAATGGTCA 
TGTGCGGATC CGCAATGTGT GAAGATATAT GACAAAGAGC AGATAGAGAG TAGCATTATC 
CAGATGGTGA GACAGAGGGA AAGAATGTAC CAGTTGCAGG ATCTAGTGTG CAATAGGTGC 
AATCAGGTGA AAGCCGCTCA TTTGACAGAG CAGTGTGAGT GTTCTGGATC CTTTAGATGC 
AAGGAGAGTG GTTCAGATTT CCACAAGAGG ATTGAGATTT TCTTGGATAT AGCAAAGCGC 
CAGAAGTTTA GACTGCTAGA AGAATGCATC TCCTGGATTT TATTTGCCAC CAGCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001325160.1
CDS	10..6429
Translation	MSGRRCDRRLNVQKVSAADELETKLGFGLFSQGETRLGWLLTFASSSWEDADTGKTFSCVDLFFVTQDGSSFKTKYKFRPYLYAATKDNMELEVEAYLRRRYERQVADIQIVHKEDLYLKNHLSGLQKKYLKVSFDTVQQLVEVKRDLLHIVERNLAKFNALEAYESILSGKREQRPQDCLDSVVDLREYDVPYHVRFAIDNDVRSGQWYNVSISSTDVILEKRTDLLQRAEVRVCAFDIETVKLPLKFPDAEYDQIMMISYMVDGQGFLITNRECVGKDIEDLEYTPKPEFEGYFKVTNVTNEVELLRKWFSHMQELKPGIYVTYNGDFFDWPFIERRASHHGIKMNEELGFRCDQNQGECRAKFVCHLDCFSWVKRDSYLPQGSQGLKAVTKVKLGYDPLEVNPEDMVRFAMEKPQTMASYSVSDAVATYYLYMTYVHPFVFSLATIIPMVPDEVLRKGSGTLCEMLLMVEAYKANVVCPNKNQADPEKFYQGKLLESETYIGGHVECLQSGVFRSDIPTSFKLDASAYQQLIDNLGRDLEYAITVEGKMRMDSVSNFDEVKEVIREKLEKLRDDPIREEGPLIYHLDVAAMYPNIILTNRLQPPSIVTDEVCTACDFNGPEKTCLRKLEWVWRGVTFKGNKSEYYHLKKQIESESVDAGANMQSSKPFLDLPKVEQQSKLKERLKKYCQKAYSRVLDKPITEVREAGICMRENPFYVDTVRSFRDRRYEYKTLNKVWKGKLSEAKASGNLIKIQEAHDMVVVYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIERIGKPLELDTDGIWCALPGSFPENFTFKTIDMKKFTISYPCVILNVDVAKNNSNDQYQTLVDPVRKTYNSRSECSIEFEVDGPYKAMIIPASKEEGILIKKRYAVFNHDGTIAELKGFEMKRRGELKLIKVFQAELFDKFLHGSTLEECYSAVAAVANRWLDLLEGQGKDIADSELLDYISESSTMSKSLADYGQQKSCAVTTAKRLADFLGDTMVKDKGLRCQYIVAREPEGTPVSERAVPVAIFQTDDPEKKFYLQKWCKISSYTGIRSIIDWMYYKQRLHSAIQKVITIPAAMQKVANPVLRVRHPYWLEKKVCDKFRQGKIVDMFSSANKDHSTTQDNVVADIEEFCKENRPSVKGPKPVARSFEVDRNHSEGKQQESWDPEFHDISLQNVDKNVDYQGWLELEKRKWKMTLTNKKKRRFDDLKPCNQIDAHKINKKVCKGRVGVGSYFRRPEEALTSSYLQIIQLVQSPQSGQFFAWVVVEGLMLKIPLTIPRVFYINSKASIAGNFTGKCINKILPHGKPCYNLMEVNIQEDQFIKESKKLAALLADPEIEGIYETKMPLEFSAICQIGCVCKIEDTAKHRNTQDGWKLGELHRITTTECRYLENSIPLVYLYHSTSTGRAVYVLYCHASKLMSVVVVNPYGDKELLSSALERQFRDRCQELSPEPFSWDGILFQVEYVDHPEAATKFLQKALCEYREENCGATVAVIECPDFNTTKEGVKALEDFPCVRIPFNDDDNSYQPVSWQRPAAKIAVLRCASAIQWLDRRIAQSRYAHVPLGNFGRDWLTFTVDIFLSRALRDQQQVLWVSDNGVPDLGDINNEETFLADETSLLFPGAYRKVSVELKVHRLAVNALLKSDLVSEMEGGGFLGVNSRGSSLNDNGSFDENNGCAQAFRVLKQLIKRLLHDACNSGNIYADSILQHLSWWLRSPSSKLHDPALHLMLHKVMQKVFALLLTDLRRLGAIIIYADFSKVIIDTGKFDLSAAKTYCESLLTVMGSRDIFKLILLEPVHYWHSLLFMDQHNYAGIRATGDEISGNEVTIEPKWSVARHLPEYIQKDFIIIVATFIFGPWKFALEKKRGSAESLEAEMVEYLKEQIGTRFISMIVEKIGNIRSHIKDINVSDASWASGQAPKGDYTFEFIQIITAVLALDQNVQQDVLVMRKILLKYIKVKECAAEAEFIDPGPSFILPNVACSNCGAYRDLDFCRDSALLTEKEWSCADPQCVKIYDKEQIESSIIQMVRQRERMYQLQDLVCNRCNQVKAAHLTEQCECSGSFRCKESGSDFHKRIEIFLDIAKRQKFRLLEECISWILFATSC

Target ORF information:

RefSeq Version	NM_001336103.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana DNA polymerase epsilon catalytic subunit (TIL2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001336103.1

ORF Insert Sequence:

ATGAGCGGAA GGAGATGTGA TCGGAGGTTG AATGTGCAGA AAGTTTCAGC GGCAGATGAA 
CTCGAAACTA AACTTGGATT TGGGCTTTTC TCCCAAGGGG AAACGCGGCT CGGGTGGCTA 
CTCACGTTTG CATCATCATC TTGGGAAGAT GCCGATACTG GGAAAACCTT TAGTTGTGTG 
GACCTTTTCT TTGTCACTCA GGACGGCTCT TCTTTTAAGA CTAAGTACAA GTTCCGCCCT 
TACTTGTATG CCGCCACCAA AGACAATATG GAACTGGAAG TTGAAGCCTA CTTGAGACGC 
CGATATGAAA GACAAGTTGC TGATATTCAG ATTGTTCACA AAGAAGATCT TTATCTTAAA 
AATCATCTAT CTGGCCTGCA AAAAAAATAT CTCAAAGTAT CATTTGACAC TGTGCAACAA 
TTGGTGGAAG TAAAGAGGGA TCTGTTGCAT ATTGTCGAAC GAAACCTCGC AAAATTTAAT 
GCACTTGAAG CTTACGAATC CATCTTGTCT GGAAAACGAG AACAACGTCC TCAAGACTGC 
TTAGACTCTG TGGTGGACCT CCGCGAGTAC GATGTTCCTT ATCATGTCCG ATTTGCAATT 
GATAATGATG TGCGTAGTGG GCAATGGTAC AATGTCAGTA TTTCCAGCAC TGATGTCATA 
CTAGAGAAGA GGACGGATCT TCTCCAACGC GCAGAAGTTC GCGTTTGTGC ATTCGATATA 
GAGACAGTAA AGCTTCCATT GAAGTTCCCG GATGCTGAAT ATGATCAAAT TATGATGATA 
TCCTACATGG TTGATGGGCA AGGTTTTCTA ATTACTAACA GAGAGTGTGT TGGCAAAGAC 
ATAGAAGATC TGGAATATAC ACCCAAACCA GAGTTTGAAG GGTATTTCAA AGTCACGAAT 
GTTACCAACG AGGTTGAACT CCTCCGAAAA TGGTTTTCTC ATATGCAAGA GTTGAAACCT 
GGTATTTATG TTACATACAA CGGTGACTTT TTTGACTGGC CATTTATAGA ACGGAGAGCA 
TCTCATCATG GGATCAAAAT GAACGAGGAG TTGGGATTCC GTTGTGATCA AAATCAAGGT 
GAATGTCGAG CTAAGTTTGT GTGCCATTTG GATTGTTTTT CTTGGGTGAA ACGTGATAGC 
TATCTTCCTC AGGGAAGCCA GGGTCTGAAG GCTGTTACAA AGGTAAAACT GGGTTACGAT 
CCACTGGAAG TGAATCCTGA GGACATGGTT CGGTTTGCAA TGGAAAAGCC ACAGACAATG 
GCCTCCTACT CTGTCTCTGA TGCTGTTGCA ACTTACTACC TATACATGAC ATATGTCCAT 
CCGTTCGTTT TTTCTCTCGC AACTATCATT CCAATGGTCC CTGATGAGGT TTTACGCAAA 
GGGAGTGGCA CCCTTTGTGA AATGCTTTTG ATGGTTGAGG CTTACAAGGC AAATGTTGTA 
TGTCCCAACA AAAATCAAGC TGACCCTGAG AAGTTCTACC AAGGTAAACT TCTTGAAAGT 
GAGACATATA TTGGTGGCCA TGTAGAGTGC CTCCAAAGTG GTGTTTTCAG ATCTGACATT 
CCAACCAGTT TTAAGCTCGA CGCATCCGCA TACCAGCAAC TGATCGATAA CCTTGGTCGG 
GATCTGGAAT ATGCTATCAC TGTTGAAGGA AAAATGAGAA TGGATTCAGT TTCCAATTTT 
GATGAAGTAA AGGAAGTAAT CAGGGAAAAG CTCGAGAAGT TACGGGATGA TCCTATACGT 
GAAGAGGGGC CTCTTATTTA TCACCTTGAT GTCGCGGCGA TGTATCCAAA TATTATCTTA 
ACAAACAGGC TTCAGCCACC ATCAATAGTT ACGGATGAGG TTTGCACAGC ATGTGATTTC 
AACGGCCCGG AAAAGACCTG TCTTAGAAAA CTTGAATGGG TTTGGCGTGG GGTGACGTTC 
AAGGGAAATA AAAGTGAATA TTACCATCTG AAGAAGCAGA TTGAGTCTGA ATCTGTTGAT 
GCTGGAGCAA ATATGCAGTC TTCAAAACCT TTTCTTGATC TGCCAAAGGT GGAACAACAA 
TCCAAATTAA AAGAACGACT GAAAAAATAC TGTCAAAAGG CATATAGCCG AGTACTTGAC 
AAGCCAATCA CTGAAGTTCG CGAGGCTGGG ATATGCATGA GAGAAAACCC GTTCTATGTG 
GACACTGTGC GCAGCTTTCG AGATAGGAGG TATGAATACA AAACACTTAA CAAGGTCTGG 
AAGGGAAAGT TGTCAGAGGC CAAGGCAAGT GGCAATTTAA TCAAGATCCA GGAAGCACAT 
GACATGGTAG TGGTTTACGA TTCCTTGCAG CTAGCTCATA AGTGTATACT TAATTCCTTT 
TATGGATATG TCATGCGAAA GGGCGCAAGA TGGTACTCCA TGGAAATGGC TGGGGTAGTT 
ACTTATACTG GAGCCAAAAT CATCCAGAAT GCTCGTTTGC TTATTGAACG AATTGGGAAA 
CCACTTGAGC TGGATACAGA TGGTATATGG TGTGCTCTAC CTGGATCTTT TCCTGAGAAC 
TTTACCTTTA AAACCATAGA TATGAAGAAA TTCACCATCT CGTATCCGTG TGTAATACTT 
AATGTTGATG TGGCGAAGAA TAATTCAAAT GACCAATATC AGACTCTAGT AGATCCTGTA 
CGAAAGACAT ATAATTCACG TAGCGAATGC TCAATTGAAT TTGAAGTGGA TGGACCGTAC 
AAGGCAATGA TTATTCCTGC ATCCAAGGAA GAGGGAATCT TAATTAAGAA GCGGTATGCT 
GTTTTCAATC ATGACGGAAC CATAGCAGAA CTCAAGGGGT TTGAGATGAA GCGTAGAGGA 
GAGCTGAAAC TCATTAAAGT TTTCCAGGCC GAGCTATTTG ACAAATTTCT GCATGGATCA 
ACCCTTGAAG AGTGTTATTC TGCTGTTGCA GCTGTTGCAA ATCGTTGGCT TGACCTACTC 
GAGGGTCAAG GAAAAGATAT TGCCGATAGT GAATTGCTAG ATTATATATC AGAATCAAGC 
ACTATGAGCA AATCGTTAGC AGATTATGGT CAACAAAAGT CATGTGCAGT GACCACAGCA 
AAACGCCTGG CTGATTTTCT TGGTGATACA ATGGTCAAAG ATAAAGGACT GCGCTGCCAA 
TACATCGTTG CTCGTGAACC AGAGGGGACA CCTGTAAGTG AACGTGCTGT CCCTGTTGCC 
ATATTTCAAA CAGATGATCC TGAAAAAAAG TTTTATCTGC AAAAATGGTG CAAGATATCA 
TCATATACTG GAATCCGTTC AATTATTGAC TGGATGTATT ACAAGCAGCG TCTTCATTCA 
GCTATTCAAA AAGTTATTAC CATTCCTGCT GCAATGCAGA AGGTGGCAAA TCCTGTACTT 
AGGGTGCGTC ATCCTTATTG GCTCGAGAAA AAGGTCTGTG ACAAATTTCG TCAGGGAAAA 
ATAGTTGATA TGTTTAGCTC AGCAAACAAA GATCATTCGA CAACTCAAGA TAATGTAGTG 
GCGGATATTG AAGAGTTTTG CAAAGAAAAC AGACCTTCCG TAAAAGGGCC AAAGCCAGTT 
GCGCGTTCAT TTGAAGTCGA TAGAAATCAC TCTGAGGGTA AGCAGCAAGA GAGTTGGGAT 
CCAGAGTTCC ATGATATCTC ATTACAGAAC GTTGACAAGA ATGTAGATTA CCAAGGATGG 
CTTGAACTGG AAAAAAGAAA ATGGAAAATG ACTCTAACGA ATAAGAAAAA AAGAAGGTTT 
GATGATCTAA AGCCTTGTAA TCAGATTGAT GCACACAAGA TAAATAAAAA AGTCTGTAAG 
GGAAGAGTAG GTGTAGGCTC ATACTTTAGG AGGCCTGAAG AAGCTTTGAC TAGTTCCTAT 
TTGCAGATAA TACAGTTGGT CCAAAGTCCA CAGAGCGGGC AATTTTTTGC TTGGGTTGTT 
GTGGAAGGAT TGATGCTTAA GATCCCATTG ACGATCCCAA GGGTGTTTTA CATTAATTCC 
AAAGCTTCTA TAGCTGGAAA CTTCACGGGA AAGTGTATCA ACAAGATTCT TCCTCACGGA 
AAGCCTTGTT ACAATTTGAT GGAGGTCAAT ATTCAGGAAG ATCAATTTAT AAAAGAAAGC 
AAAAAGCTTG CCGCTCTACT TGCAGACCCC GAAATTGAGG GCATATATGA AACTAAGATG 
CCTCTGGAGT TTAGTGCTAT ATGTCAGATT GGATGTGTAT GCAAAATTGA AGACACGGCC 
AAACACCGTA ACACCCAAGA TGGATGGAAA CTTGGTGAAC TTCACAGGAT AACTACAACT 
GAGTGTCGCT ACTTGGAGAA TTCTATTCCA CTTGTTTATT TGTATCACAG CACCTCAACA 
GGTCGTGCTG TTTATGTTTT ATATTGTCAT GCTTCGAAGC TTATGTCTGT TGTGGTAGTC 
AATCCTTACG GTGACAAGGA ATTATTATCA TCTGCCCTAG AGAGACAGTT TAGAGATAGA 
TGCCAAGAAC TGTCACCTGA GCCATTTTCT TGGGATGGTA TCCTTTTCCA GGTCGAGTAT 
GTTGACCATC CAGAAGCTGC CACGAAATTT TTACAAAAAG CGCTCTGTGA ATACAGAGAA 
GAAAATTGTG GAGCAACTGT GGCAGTCATT GAATGCCCTG ACTTTAACAC CACGAAGGAA 
GGTGTAAAGG CCCTGGAAGA TTTCCCATGT GTGAGGATTC CCTTCAACGA CGATGACAAT 
AGTTATCAGC CTGTCTCCTG GCAACGTCCA GCAGCAAAAA TAGCAGTGCT CCGTTGTGCT 
TCAGCAATTC AGTGGTTGGA CCGGAGGATT GCCCAGTCAA GATACGCCCA TGTGCCTCTG 
GGGAATTTTG GACGTGATTG GTTAACGTTC ACAGTAGATA TCTTCCTGTC CAGGGCACTC 
CGTGACCAGC AACAGGTTCT TTGGGTCTCA GATAATGGTG TTCCAGACCT TGGAGATATA 
AACAATGAAG AGACATTTCT AGCTGATGAG ACAAGTTTGC TATTCCCTGG AGCGTACAGA 
AAAGTGTCTG TGGAGTTAAA GGTCCATAGA TTAGCTGTTA ATGCCCTTCT GAAAAGTGAC 
CTGGTGAGTG AAATGGAAGG AGGCGGCTTT TTGGGTGTGA ATTCTAGAGG AAGTAGCTTA 
AACGATAATG GCAGTTTTGA TGAGAATAAT GGATGCGCGC AAGCATTTCG TGTCCTGAAA 
CAATTGATCA AGCGCTTGTT GCATGATGCG TGTAATTCTG GCAATATATA TGCTGATTCC 
ATTTTGCAAC ATCTGTCTTG GTGGCTCCGC AGCCCTTCCT CAAAGCTTCA CGATCCAGCT 
CTCCATCTCA TGCTTCACAA GGTCATGCAA AAGGTGTTTG CGCTGCTTTT GACTGATTTG 
CGGAGGTTAG GTGCTATAAT AATATACGCA GACTTCTCAA AGGTCATTAT CGACACAGGG 
AAATTTGATC TATCTGCTGC AAAGACTTAT TGTGAAAGCT TGCTCACAGT GATGGGGAGT 
AGAGATATCT TTAAGTTGAT CTTGCTTGAG CCGGTTCACT ACTGGCACTC GTTACTTTTC 
ATGGACCAGC ATAACTACGC TGGTATCCGA GCTACCGGAG ATGAAATTTC AGGTAACGAA 
GTTACTATTG AACCAAAGTG GAGCGTTGCA CGGCATTTAC CCGAGTATAT TCAGAAGGAT 
TTTATCATAA TTGTTGCCAC GTTTATATTT GGCCCCTGGA AATTTGCATT AGAGAAAAAA 
AGGGGCAGTG CAGAGAGTTT AGAGGCAGAG ATGGTAGAAT ATCTCAAGGA ACAGATAGGA 
ACCCGGTTCA TAAGCATGAT TGTTGAGAAG ATTGGTAATA TCAGGTCACA CATCAAGGAT 
ATAAACGTGT CAGATGCATC TTGGGCTTCT GGTCAAGCTC CCAAAGGAGA TTACACGTTC 
GAATTTATAC AAATTATTAC TGCTGTTCTG GCTCTTGATC AAAACGTTCA GCAAGATGTT 
CTGGTAATGA GAAAGATTCT GTTGAAGTAC ATTAAAGTAA AAGAATGTGC TGCTGAAGCC 
GAATTTATTG ATCCTGGGCC ATCCTTCATC TTACCCAACG TGGCTTGCAG CAACTGCGGT 
GCCTACAGAG ACCTAGATTT CTGCAGAGAC TCAGCTCTCT TAACAGAAAA AGAATGGTCA 
TGTGCGGATC CGCAATGTGT GAAGATATAT GACAAAGAGC AGATAGAGAG TAGCATTATC 
CAGATGGTGA GACAGAGGGA AAGAATGTAC CAGTTGCAGG ATCTAGTGTG CAATAGGTGC 
AATCAGGTGA AAGCCGCTCA TTTGACAGAG CAGTGTGAGT GTTCTGGATC CTTTAGATGC 
AAGGAGAGTG GTTCAGATTT CCACAAGAGG ATTGAGATTT TCTTGGATAT AGCAAAGCGC 
CAGAAGTTTA GACTGCTAGA AGAATGCATC TCCTGGATTT TATTTGCCAC CAGCTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIL2 ( NM_128270.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_180280.2 Arabidopsis thaliana DNA polymerase epsilon catalytic subunit (TIL2), partial mRNA.

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CloneID	OAb08111
Clone ID Related Accession (Same CDS sequence)	NM_128270.3
Accession Version	NM_128270.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	DNA polymerase epsilon catalytic subunit
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_128270.2. COMPLETENESS: incomplete on the 3' end.

ATGAGCGGAA GGAGATGTGA TCGGAGGTTG AATGTGCAGA AAGTTTCAGC GGCAGATGAA 
CTCGAAACTA AACTTGGATT TGGGCTTTTC TCCCAAGGGG AAACGCGGCT CGGGTGGCTA 
CTCACGTTTG CATCATCATC TTGGGAAGAT GCCGATACTG GGAAAACCTT TAGTTGTGTG 
GACCTTTTCT TTGTCACTCA GGACGGCTCT TCTTTTAAGA CTAAGTACAA GTTCCGCCCT 
TACTTGTATG CCGCCACCAA AGACAATATG GAACTGGAAG TTGAAGCCTA CTTGAGACGC 
CGATATGAAA GACAAGTTGC TGATATTCAG ATTGTTCACA AAGAAGATCT TTATCTTAAA 
AATCATCTAT CTGGCCTGCA AAAAAAATAT CTCAAAGTAT CATTTGACAC TGTGCAACAA 
TTGGTGGAAG TAAAGAGGGA TCTGTTGCAT ATTGTCGAAC GAAACCTCGC AAAATTTAAT 
GCACTTGAAG CTTACGAATC CATCTTGTCT GGAAAACGAG AACAACGTCC TCAAGACTGC 
TTAGACTCTG TGGTGGACCT CCGCGAGTAC GATGTTCCTT ATCATGTCCG ATTTGCAATT 
GATAATGATG TGCGTAGTGG GCAATGGTAC AATGTCAGTA TTTCCAGCAC TGATGTCATA 
CTAGAGAAGA GGACGGATCT TCTCCAACGC GCAGAAGTTC GCGTTTGTGC ATTCGATATA 
GAGACAGTAA AGCTTCCATT GAAGTTCCCG GATGCTGAAT ATGATCAAAT TATGATGATA 
TCCTACATGG TTGATGGGCA AGGTTTTCTA ATTACTAACA GAGAGTGTGT TGGCAAAGAC 
ATAGAAGATC TGGAATATAC ACCCAAACCA GAGTTTGAAG GGTATTTCAA AGTCACGAAT 
GTTACCAACG AGGTTGAACT CCTCCGAAAA TGGTTTTCTC ATATGCAAGA GTTGAAACCT 
GGTATTTATG TTACATACAA CGGTGACTTT TTTGACTGGC CATTTATAGA ACGGAGAGCA 
TCTCATCATG GGATCAAAAT GAACGAGGAG TTGGGATTCC GTTGTGATCA AAATCAAGGT 
GAATGTCGAG CTAAGTTTGT GTGCCATTTG GATTGTTTTT CTTGGGTGAA ACGTGATAGC 
TATCTTCCTC AGGGAAGCCA GGGTCTGAAG GCTGTTACAA AGGTAAAACT GGGTTACGAT 
CCACTGGAAG TGAATCCTGA GGACATGGTT CGGTTTGCAA TGGAAAAGCC ACAGACAATG 
GCCTCCTACT CTGTCTCTGA TGCTGTTGCA ACTTACTACC TATACATGAC ATATGTCCAT 
CCGTTCGTTT TTTCTCTCGC AACTATCATT CCAATGGTCC CTGATGAGGT TTTACGCAAA 
GGGAGTGGCA CCCTTTGTGA AATGCTTTTG ATGGTTGAGG CTTACAAGGC AAATGTTGTA 
TGTCCCAACA AAAATCAAGC TGACCCTGAG AAGTTCTACC AAGGTAAACT TCTTGAAAGT 
GAGACATATA TTGGTGGCCA TGTAGAGTGC CTCCAAAGTG GTGTTTTCAG ATCTGACATT 
CCAACCAGTT TTAAGCTCGA CGCATCCGCA TACCAGCAAC TGATCGATAA CCTTGGTCGG 
GATCTGGAAT ATGCTATCAC TGTTGAAGGA AAAATGAGAA TGGATTCAGT TTCCAATTTT 
GATGAAGTAA AGGAAGTAAT CAGGGAAAAG CTCGAGAAGT TACGGGATGA TCCTATACGT 
GAAGAGGGGC CTCTTATTTA TCACCTTGAT GTCGCGGCGA TGTATCCAAA TATTATCTTA 
ACAAACAGGC TTCAGCCACC ATCAATAGTT ACGGATGAGG TTTGCACAGC ATGTGATTTC 
AACGGCCCGG AAAAGACCTG TCTTAGAAAA CTTGAATGGG TTTGGCGTGG GGTGACGTTC 
AAGGGAAATA AAAGTGAATA TTACCATCTG AAGAAGCAGA TTGAGTCTGA ATCTGTTGAT 
GCTGGAGCAA ATATGCAGTC TTCAAAACCT TTTCTTGATC TGCCAAAGGT GGAACAACAA 
TCCAAATTAA AAGAACGACT GAAAAAATAC TGTCAAAAGG CATATAGCCG AGTACTTGAC 
AAGCCAATCA CTGAAGTTCG CGAGGCTGGG ATATGCATGA GAGAAAACCC GTTCTATGTG 
GACACTGTGC GCAGCTTTCG AGATAGGAGG TATGAATACA AAACACTTAA CAAGGTCTGG 
AAGGGAAAGT TGTCAGAGGC CAAGGCAAGT GGCAATTTAA TCAAGATCCA GGAAGCACAT 
GACATGGTAG TGGTTTACGA TTCCTTGCAG CTAGCTCATA AGTGTATACT TAATTCCTTT 
TATGGATATG TCATGCGAAA GGGCGCAAGA TGGTACTCCA TGGAAATGGC TGGGGTAGTT 
ACTTATACTG GAGCCAAAAT CATCCAGAAT GCTCGTTTGC TTATTGAACG AATTGGGAAA 
CCACTTGAGC TGGATACAGA TGGTATATGG TGTGCTCTAC CTGGATCTTT TCCTGAGAAC 
TTTACCTTTA AAACCATAGA TATGAAGAAA TTCACCATCT CGTATCCGTG TGTAATACTT 
AATGTTGATG TGGCGAAGAA TAATTCAAAT GACCAATATC AGACTCTAGT AGATCCTGTA 
CGAAAGACAT ATAATTCACG TAGCGAATGC TCAATTGAAT TTGAAGTGGA TGGACCGTAC 
AAGGCAATGA TTATTCCTGC ATCCAAGGAA GAGGGAATCT TAATTAAGAA GCGGTATGCT 
GTTTTCAATC ATGACGGAAC CATAGCAGAA CTCAAGGGGT TTGAGATGAA GCGTAGAGGA 
GAGCTGAAAC TCATTAAAGT TTTCCAGGCC GAGCTATTTG ACAAATTTCT GCATGGATCA 
ACCCTTGAAG AGTGTTATTC TGCTGTTGCA GCTGTTGCAA ATCGTTGGCT TGACCTACTC 
GAGGGTCAAG GAAAAGATAT TGCCGATAGT GAATTGCTAG ATTATATATC AGAATCAAGC 
ACTATGAGCA AATCGTTAGC AGATTATGGT CAACAAAAGT CATGTGCAGT GACCACAGCA 
AAACGCCTGG CTGATTTTCT TGGTGATACA ATGGTCAAAG ATAAAGGACT GCGCTGCCAA 
TACATCGTTG CTCGTGAACC AGAGGGGACA CCTGTAAGTG AACGTGCTGT CCCTGTTGCC 
ATATTTCAAA CAGATGATCC TGAAAAAAAG TTTTATCTGC AAAAATGGTG CAAGATATCA 
TCATATACTG GAATCCGTTC AATTATTGAC TGGATGTATT ACAAGCAGCG TCTTCATTCA 
GCTATTCAAA AAGTTATTAC CATTCCTGCT GCAATGCAGA AGGTGGCAAA TCCTGTACTT 
AGGGTGCGTC ATCCTTATTG GCTCGAGAAA AAGGTCTGTG ACAAATTTCG TCAGGGAAAA 
ATAGTTGATA TGTTTAGCTC AGCAAACAAA GATCATTCGA CAACTCAAGA TAATGTAGTG 
GCGGATATTG AAGAGTTTTG CAAAGAAAAC AGACCTTCCG TAAAAGGGCC AAAGCCAGTT 
GCGCGTTCAT TTGAAGTCGA TAGAAATCAC TCTGAGGGTA AGCAGCAAGA GAGTTGGGAT 
CCAGAGTTCC ATGATATCTC ATTACAGAAC GTTGACAAGA ATGTAGATTA CCAAGGATGG 
CTTGAACTGG AAAAAAGAAA ATGGAAAATG ACTCTAACGA ATAAGAAAAA AAGAAGGTAC 
TCCTCAAGTT TATTTGGTTT TGATTTGGAA CAAAATATAA ATAAAAAAGT CTGTAAGGGA 
AGAGTAGGTG TAGGCTCATA CTTTAGGAGG CCTGAAGAAG CTTTGACTAG TTCCTATTTG 
CAGATAATAC AGTTGGTCCA AAGTCCACAG AGCGGGCAAT TTTTTGCTTG GGTTGTTGTG 
GAAGGATTGA TGCTTAAGAT CCCATTGACG ATCCCAAGGG TGTTTTACAT TAATTCCAAA 
GCTTCTATAG CTGGAAACTT CACGGGAAAG TGTATCAACA AGATTCTTCC TCACGGAAAG 
CCTTGTTACA ATTTGATGGA GGTCAATATT CAGGAAGATC AATTTATAAA AGAAAGCAAA 
AAGCTTGCCG CTCTACTTGC AGACCCCGAA ATTGAGGGCA TATATGAAAC TAAGATGCCT 
CTGGAGTTTA GTGCTATATG TCAGATTGGA TGTGTATGCA AAATTGAAGA CACGGCCAAA 
CACCGTAACA CCCAAGATGG ATGGAAACTT GGTGAACTTC ACAGGATAAC TACAACTGAG 
TGTCGCTACT TGGAGAATTC TATTCCACTT GTTTATTTGT ATCACAGCAC CTCAACAGGT 
CGTGCTGTTT ATGTTTTATA TTGTCATGCT TCGAAGCTTA TGTCTGTTGT GGTAGTCAAT 
CCTTACGGTG ACAAGGAATT ATTATCATCT GCCCTAGAGA GACAGTTTAG AGATAGATGC 
CAAGAACTGT CACCTGAGCC ATTTTCTTGG GATGGTATCC TTTTCCAGGT CGAGTATGTT 
GACCATCCAG AAGCTGCCAC GAAATTTTTA CAAAAAGCGC TCTGTGAATA CAGAGAAGAA 
AATTGTGGAG CAACTGTGGC AGTCATTGAA TGCCCTGACT TTAACACCAC GAAGGAAGGT 
GTAAAGGCCC TGGAAGATTT CCCATGTGTG AGGATTCCCT TCAACGACGA TGACAATAGT 
TATCAGCCTG TCTCCTGGCA ACGTCCAGCA GCAAAAATAG CAGTGCTCCG TTGTGCTTCA 
GCAATTCAGT GGTTGGACCG GAGGATTGCC CAGTCAAGAT ACGCCCATGT GCCTCTGGGG 
AATTTTGGAC GTGATTGGTT AACGTTCACA GTAGATATCT TCCTGTCCAG GGCACTCCGT 
GACCAGCAAC AGGTTCTTTG GGTCTCAGAT AATGGTGTTC CAGACCTTGG AGATATAAAC 
AATGAAGAGA CATTTCTAGC TGATGAGACA AGTTTGCTAT TCCCTGGAGC GTACAGAAAA 
GTGTCTGTGG AGTTAAAGGT CCATAGATTA GCTGTTAATG CCCTTCTGAA AAGTGACCTG 
GTGAGTGAAA TGGAAGGAGG CGGCTTTTTG GGTGTGAATT CTAGAGGAAG TAGCTTAAAC 
GATAATGGCA GTTTTGATGA GAATAATGGA TGCGCGCAAG CATTTCGTGT CCTGAAACAA 
TTGATCAAGC GCTTGTTGCA TGATGCGTGT AATTCTGGCA ATATATATGC TGATTCCATT 
TTGCAACATC TGTCTTGGTG GCTCCGCAGC CCTTCCTCAA AGCTTCACGA TCCAGCTCTC 
CATCTCATGC TTCACAAGGT CATGCAAAAG GTGTTTGCGC TGCTTTTGAC TGATTTGCGG 
AGGTTAGGTG CTATAATAAT ATACGCAGAC TTCTCAAAGG TCATTATCGA CACAGGGAAA 
TTTGATCTAT CTGCTGCAAA GACTTATTGT GAAAGCTTGC TCACAGTGAT GGGGAGTAGA 
GATATCTTTA AGTTGATCTT GCTTGAGCCG GTTCACTACT GGCACTCGTT ACTTTTCATG 
GACCAGCATA ACTACGCTGG TATCCGAGCT ACCGGAGATG AAATTTCAGG TAACGAAGTT 
ACTATTGAAC CAAAGTGGAG CGTTGCACGG CATTTACCCG AGTATATTCA GAAGGATTTT 
ATCATAATTG TTGCCACGTT TATATTTGGC CCCTGGAAAT TTGCATTAGA GAAAAAAAGG 
GGCAGTGCAG AGAGTTTAGA GGCAGAGATG GTAGAATATC TCAAGGAACA GATAGGAACC 
CGGTTCATAA GCATGATTGT TGAGAAGATT GGTAATATCA GGTCACACAT CAAGGATATA 
AACGTGTCAG ATGCATCTTG GGCTTCTGGT CAAGCTCCCA AAGGAGATTA CACGTTCGAA 
TTTATACAAA TTATTACTGC TGTTCTGGCT CTTGATCAAA ACGTTCAGCA AGATGTTCTG 
GTAATGAGAA AGATTCTGTT GAAGTACATT AAAGTAAAAG AATGTGCTGC TGAAGCCGAA 
TTTATTGATC CTGGGCCATC CTTCATCTTA CCCAACGTGG CTTGCAGCAA CTGCGGTGCC 
TACAGAGACC TAGATTTCTG CAGAGACTCA GCTCTCTTAA CAGAAAAAGA ATGGTCATGT 
GCGGATCCGC AATGTGTGAA GATATATGAC AAAGAGCAGA TAGAGAGTAG CATTATCCAG 
ATGGTGAGAC AGAGGGAAAG AATGTACCAG TTGCAGGATC TAGTGTGCAA TAGGTGCAAT 
CAGGTGAAAG CCGCTCATTT GACAGAGCAG TGTGAGTGTT CTGGATCCTT TAGATGCAAG 
GAGAGTGGTT CAGATTTCCA CAAGAGGATT GAGATTTTCT TGGATATAGC AAAGCGCCAG 
AAGTTTAGAC TGCTAGAAGA ATGCATCTCC TGGATTTTAT TTGCCACCAG CTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_180280.2
CDS	10..6426
Translation	MSGRRCDRRLNVQKVSAADELETKLGFGLFSQGETRLGWLLTFASSSWEDADTGKTFSCVDLFFVTQDGSSFKTKYKFRPYLYAATKDNMELEVEAYLRRRYERQVADIQIVHKEDLYLKNHLSGLQKKYLKVSFDTVQQLVEVKRDLLHIVERNLAKFNALEAYESILSGKREQRPQDCLDSVVDLREYDVPYHVRFAIDNDVRSGQWYNVSISSTDVILEKRTDLLQRAEVRVCAFDIETVKLPLKFPDAEYDQIMMISYMVDGQGFLITNRECVGKDIEDLEYTPKPEFEGYFKVTNVTNEVELLRKWFSHMQELKPGIYVTYNGDFFDWPFIERRASHHGIKMNEELGFRCDQNQGECRAKFVCHLDCFSWVKRDSYLPQGSQGLKAVTKVKLGYDPLEVNPEDMVRFAMEKPQTMASYSVSDAVATYYLYMTYVHPFVFSLATIIPMVPDEVLRKGSGTLCEMLLMVEAYKANVVCPNKNQADPEKFYQGKLLESETYIGGHVECLQSGVFRSDIPTSFKLDASAYQQLIDNLGRDLEYAITVEGKMRMDSVSNFDEVKEVIREKLEKLRDDPIREEGPLIYHLDVAAMYPNIILTNRLQPPSIVTDEVCTACDFNGPEKTCLRKLEWVWRGVTFKGNKSEYYHLKKQIESESVDAGANMQSSKPFLDLPKVEQQSKLKERLKKYCQKAYSRVLDKPITEVREAGICMRENPFYVDTVRSFRDRRYEYKTLNKVWKGKLSEAKASGNLIKIQEAHDMVVVYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGVVTYTGAKIIQNARLLIERIGKPLELDTDGIWCALPGSFPENFTFKTIDMKKFTISYPCVILNVDVAKNNSNDQYQTLVDPVRKTYNSRSECSIEFEVDGPYKAMIIPASKEEGILIKKRYAVFNHDGTIAELKGFEMKRRGELKLIKVFQAELFDKFLHGSTLEECYSAVAAVANRWLDLLEGQGKDIADSELLDYISESSTMSKSLADYGQQKSCAVTTAKRLADFLGDTMVKDKGLRCQYIVAREPEGTPVSERAVPVAIFQTDDPEKKFYLQKWCKISSYTGIRSIIDWMYYKQRLHSAIQKVITIPAAMQKVANPVLRVRHPYWLEKKVCDKFRQGKIVDMFSSANKDHSTTQDNVVADIEEFCKENRPSVKGPKPVARSFEVDRNHSEGKQQESWDPEFHDISLQNVDKNVDYQGWLELEKRKWKMTLTNKKKRRYSSSLFGFDLEQNINKKVCKGRVGVGSYFRRPEEALTSSYLQIIQLVQSPQSGQFFAWVVVEGLMLKIPLTIPRVFYINSKASIAGNFTGKCINKILPHGKPCYNLMEVNIQEDQFIKESKKLAALLADPEIEGIYETKMPLEFSAICQIGCVCKIEDTAKHRNTQDGWKLGELHRITTTECRYLENSIPLVYLYHSTSTGRAVYVLYCHASKLMSVVVVNPYGDKELLSSALERQFRDRCQELSPEPFSWDGILFQVEYVDHPEAATKFLQKALCEYREENCGATVAVIECPDFNTTKEGVKALEDFPCVRIPFNDDDNSYQPVSWQRPAAKIAVLRCASAIQWLDRRIAQSRYAHVPLGNFGRDWLTFTVDIFLSRALRDQQQVLWVSDNGVPDLGDINNEETFLADETSLLFPGAYRKVSVELKVHRLAVNALLKSDLVSEMEGGGFLGVNSRGSSLNDNGSFDENNGCAQAFRVLKQLIKRLLHDACNSGNIYADSILQHLSWWLRSPSSKLHDPALHLMLHKVMQKVFALLLTDLRRLGAIIIYADFSKVIIDTGKFDLSAAKTYCESLLTVMGSRDIFKLILLEPVHYWHSLLFMDQHNYAGIRATGDEISGNEVTIEPKWSVARHLPEYIQKDFIIIVATFIFGPWKFALEKKRGSAESLEAEMVEYLKEQIGTRFISMIVEKIGNIRSHIKDINVSDASWASGQAPKGDYTFEFIQIITAVLALDQNVQQDVLVMRKILLKYIKVKECAAEAEFIDPGPSFILPNVACSNCGAYRDLDFCRDSALLTEKEWSCADPQCVKIYDKEQIESSIIQMVRQRERMYQLQDLVCNRCNQVKAAHLTEQCECSGSFRCKESGSDFHKRIEIFLDIAKRQKFRLLEECISWILFATSC

Target ORF information:

RefSeq Version	NM_128270.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana DNA polymerase epsilon catalytic subunit (TIL2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_128270.3

ORF Insert Sequence:

ATGAGCGGAA GGAGATGTGA TCGGAGGTTG AATGTGCAGA AAGTTTCAGC GGCAGATGAA 
CTCGAAACTA AACTTGGATT TGGGCTTTTC TCCCAAGGGG AAACGCGGCT CGGGTGGCTA 
CTCACGTTTG CATCATCATC TTGGGAAGAT GCCGATACTG GGAAAACCTT TAGTTGTGTG 
GACCTTTTCT TTGTCACTCA GGACGGCTCT TCTTTTAAGA CTAAGTACAA GTTCCGCCCT 
TACTTGTATG CCGCCACCAA AGACAATATG GAACTGGAAG TTGAAGCCTA CTTGAGACGC 
CGATATGAAA GACAAGTTGC TGATATTCAG ATTGTTCACA AAGAAGATCT TTATCTTAAA 
AATCATCTAT CTGGCCTGCA AAAAAAATAT CTCAAAGTAT CATTTGACAC TGTGCAACAA 
TTGGTGGAAG TAAAGAGGGA TCTGTTGCAT ATTGTCGAAC GAAACCTCGC AAAATTTAAT 
GCACTTGAAG CTTACGAATC CATCTTGTCT GGAAAACGAG AACAACGTCC TCAAGACTGC 
TTAGACTCTG TGGTGGACCT CCGCGAGTAC GATGTTCCTT ATCATGTCCG ATTTGCAATT 
GATAATGATG TGCGTAGTGG GCAATGGTAC AATGTCAGTA TTTCCAGCAC TGATGTCATA 
CTAGAGAAGA GGACGGATCT TCTCCAACGC GCAGAAGTTC GCGTTTGTGC ATTCGATATA 
GAGACAGTAA AGCTTCCATT GAAGTTCCCG GATGCTGAAT ATGATCAAAT TATGATGATA 
TCCTACATGG TTGATGGGCA AGGTTTTCTA ATTACTAACA GAGAGTGTGT TGGCAAAGAC 
ATAGAAGATC TGGAATATAC ACCCAAACCA GAGTTTGAAG GGTATTTCAA AGTCACGAAT 
GTTACCAACG AGGTTGAACT CCTCCGAAAA TGGTTTTCTC ATATGCAAGA GTTGAAACCT 
GGTATTTATG TTACATACAA CGGTGACTTT TTTGACTGGC CATTTATAGA ACGGAGAGCA 
TCTCATCATG GGATCAAAAT GAACGAGGAG TTGGGATTCC GTTGTGATCA AAATCAAGGT 
GAATGTCGAG CTAAGTTTGT GTGCCATTTG GATTGTTTTT CTTGGGTGAA ACGTGATAGC 
TATCTTCCTC AGGGAAGCCA GGGTCTGAAG GCTGTTACAA AGGTAAAACT GGGTTACGAT 
CCACTGGAAG TGAATCCTGA GGACATGGTT CGGTTTGCAA TGGAAAAGCC ACAGACAATG 
GCCTCCTACT CTGTCTCTGA TGCTGTTGCA ACTTACTACC TATACATGAC ATATGTCCAT 
CCGTTCGTTT TTTCTCTCGC AACTATCATT CCAATGGTCC CTGATGAGGT TTTACGCAAA 
GGGAGTGGCA CCCTTTGTGA AATGCTTTTG ATGGTTGAGG CTTACAAGGC AAATGTTGTA 
TGTCCCAACA AAAATCAAGC TGACCCTGAG AAGTTCTACC AAGGTAAACT TCTTGAAAGT 
GAGACATATA TTGGTGGCCA TGTAGAGTGC CTCCAAAGTG GTGTTTTCAG ATCTGACATT 
CCAACCAGTT TTAAGCTCGA CGCATCCGCA TACCAGCAAC TGATCGATAA CCTTGGTCGG 
GATCTGGAAT ATGCTATCAC TGTTGAAGGA AAAATGAGAA TGGATTCAGT TTCCAATTTT 
GATGAAGTAA AGGAAGTAAT CAGGGAAAAG CTCGAGAAGT TACGGGATGA TCCTATACGT 
GAAGAGGGGC CTCTTATTTA TCACCTTGAT GTCGCGGCGA TGTATCCAAA TATTATCTTA 
ACAAACAGGC TTCAGCCACC ATCAATAGTT ACGGATGAGG TTTGCACAGC ATGTGATTTC 
AACGGCCCGG AAAAGACCTG TCTTAGAAAA CTTGAATGGG TTTGGCGTGG GGTGACGTTC 
AAGGGAAATA AAAGTGAATA TTACCATCTG AAGAAGCAGA TTGAGTCTGA ATCTGTTGAT 
GCTGGAGCAA ATATGCAGTC TTCAAAACCT TTTCTTGATC TGCCAAAGGT GGAACAACAA 
TCCAAATTAA AAGAACGACT GAAAAAATAC TGTCAAAAGG CATATAGCCG AGTACTTGAC 
AAGCCAATCA CTGAAGTTCG CGAGGCTGGG ATATGCATGA GAGAAAACCC GTTCTATGTG 
GACACTGTGC GCAGCTTTCG AGATAGGAGG TATGAATACA AAACACTTAA CAAGGTCTGG 
AAGGGAAAGT TGTCAGAGGC CAAGGCAAGT GGCAATTTAA TCAAGATCCA GGAAGCACAT 
GACATGGTAG TGGTTTACGA TTCCTTGCAG CTAGCTCATA AGTGTATACT TAATTCCTTT 
TATGGATATG TCATGCGAAA GGGCGCAAGA TGGTACTCCA TGGAAATGGC TGGGGTAGTT 
ACTTATACTG GAGCCAAAAT CATCCAGAAT GCTCGTTTGC TTATTGAACG AATTGGGAAA 
CCACTTGAGC TGGATACAGA TGGTATATGG TGTGCTCTAC CTGGATCTTT TCCTGAGAAC 
TTTACCTTTA AAACCATAGA TATGAAGAAA TTCACCATCT CGTATCCGTG TGTAATACTT 
AATGTTGATG TGGCGAAGAA TAATTCAAAT GACCAATATC AGACTCTAGT AGATCCTGTA 
CGAAAGACAT ATAATTCACG TAGCGAATGC TCAATTGAAT TTGAAGTGGA TGGACCGTAC 
AAGGCAATGA TTATTCCTGC ATCCAAGGAA GAGGGAATCT TAATTAAGAA GCGGTATGCT 
GTTTTCAATC ATGACGGAAC CATAGCAGAA CTCAAGGGGT TTGAGATGAA GCGTAGAGGA 
GAGCTGAAAC TCATTAAAGT TTTCCAGGCC GAGCTATTTG ACAAATTTCT GCATGGATCA 
ACCCTTGAAG AGTGTTATTC TGCTGTTGCA GCTGTTGCAA ATCGTTGGCT TGACCTACTC 
GAGGGTCAAG GAAAAGATAT TGCCGATAGT GAATTGCTAG ATTATATATC AGAATCAAGC 
ACTATGAGCA AATCGTTAGC AGATTATGGT CAACAAAAGT CATGTGCAGT GACCACAGCA 
AAACGCCTGG CTGATTTTCT TGGTGATACA ATGGTCAAAG ATAAAGGACT GCGCTGCCAA 
TACATCGTTG CTCGTGAACC AGAGGGGACA CCTGTAAGTG AACGTGCTGT CCCTGTTGCC 
ATATTTCAAA CAGATGATCC TGAAAAAAAG TTTTATCTGC AAAAATGGTG CAAGATATCA 
TCATATACTG GAATCCGTTC AATTATTGAC TGGATGTATT ACAAGCAGCG TCTTCATTCA 
GCTATTCAAA AAGTTATTAC CATTCCTGCT GCAATGCAGA AGGTGGCAAA TCCTGTACTT 
AGGGTGCGTC ATCCTTATTG GCTCGAGAAA AAGGTCTGTG ACAAATTTCG TCAGGGAAAA 
ATAGTTGATA TGTTTAGCTC AGCAAACAAA GATCATTCGA CAACTCAAGA TAATGTAGTG 
GCGGATATTG AAGAGTTTTG CAAAGAAAAC AGACCTTCCG TAAAAGGGCC AAAGCCAGTT 
GCGCGTTCAT TTGAAGTCGA TAGAAATCAC TCTGAGGGTA AGCAGCAAGA GAGTTGGGAT 
CCAGAGTTCC ATGATATCTC ATTACAGAAC GTTGACAAGA ATGTAGATTA CCAAGGATGG 
CTTGAACTGG AAAAAAGAAA ATGGAAAATG ACTCTAACGA ATAAGAAAAA AAGAAGGTAC 
TCCTCAAGTT TATTTGGTTT TGATTTGGAA CAAAATATAA ATAAAAAAGT CTGTAAGGGA 
AGAGTAGGTG TAGGCTCATA CTTTAGGAGG CCTGAAGAAG CTTTGACTAG TTCCTATTTG 
CAGATAATAC AGTTGGTCCA AAGTCCACAG AGCGGGCAAT TTTTTGCTTG GGTTGTTGTG 
GAAGGATTGA TGCTTAAGAT CCCATTGACG ATCCCAAGGG TGTTTTACAT TAATTCCAAA 
GCTTCTATAG CTGGAAACTT CACGGGAAAG TGTATCAACA AGATTCTTCC TCACGGAAAG 
CCTTGTTACA ATTTGATGGA GGTCAATATT CAGGAAGATC AATTTATAAA AGAAAGCAAA 
AAGCTTGCCG CTCTACTTGC AGACCCCGAA ATTGAGGGCA TATATGAAAC TAAGATGCCT 
CTGGAGTTTA GTGCTATATG TCAGATTGGA TGTGTATGCA AAATTGAAGA CACGGCCAAA 
CACCGTAACA CCCAAGATGG ATGGAAACTT GGTGAACTTC ACAGGATAAC TACAACTGAG 
TGTCGCTACT TGGAGAATTC TATTCCACTT GTTTATTTGT ATCACAGCAC CTCAACAGGT 
CGTGCTGTTT ATGTTTTATA TTGTCATGCT TCGAAGCTTA TGTCTGTTGT GGTAGTCAAT 
CCTTACGGTG ACAAGGAATT ATTATCATCT GCCCTAGAGA GACAGTTTAG AGATAGATGC 
CAAGAACTGT CACCTGAGCC ATTTTCTTGG GATGGTATCC TTTTCCAGGT CGAGTATGTT 
GACCATCCAG AAGCTGCCAC GAAATTTTTA CAAAAAGCGC TCTGTGAATA CAGAGAAGAA 
AATTGTGGAG CAACTGTGGC AGTCATTGAA TGCCCTGACT TTAACACCAC GAAGGAAGGT 
GTAAAGGCCC TGGAAGATTT CCCATGTGTG AGGATTCCCT TCAACGACGA TGACAATAGT 
TATCAGCCTG TCTCCTGGCA ACGTCCAGCA GCAAAAATAG CAGTGCTCCG TTGTGCTTCA 
GCAATTCAGT GGTTGGACCG GAGGATTGCC CAGTCAAGAT ACGCCCATGT GCCTCTGGGG 
AATTTTGGAC GTGATTGGTT AACGTTCACA GTAGATATCT TCCTGTCCAG GGCACTCCGT 
GACCAGCAAC AGGTTCTTTG GGTCTCAGAT AATGGTGTTC CAGACCTTGG AGATATAAAC 
AATGAAGAGA CATTTCTAGC TGATGAGACA AGTTTGCTAT TCCCTGGAGC GTACAGAAAA 
GTGTCTGTGG AGTTAAAGGT CCATAGATTA GCTGTTAATG CCCTTCTGAA AAGTGACCTG 
GTGAGTGAAA TGGAAGGAGG CGGCTTTTTG GGTGTGAATT CTAGAGGAAG TAGCTTAAAC 
GATAATGGCA GTTTTGATGA GAATAATGGA TGCGCGCAAG CATTTCGTGT CCTGAAACAA 
TTGATCAAGC GCTTGTTGCA TGATGCGTGT AATTCTGGCA ATATATATGC TGATTCCATT 
TTGCAACATC TGTCTTGGTG GCTCCGCAGC CCTTCCTCAA AGCTTCACGA TCCAGCTCTC 
CATCTCATGC TTCACAAGGT CATGCAAAAG GTGTTTGCGC TGCTTTTGAC TGATTTGCGG 
AGGTTAGGTG CTATAATAAT ATACGCAGAC TTCTCAAAGG TCATTATCGA CACAGGGAAA 
TTTGATCTAT CTGCTGCAAA GACTTATTGT GAAAGCTTGC TCACAGTGAT GGGGAGTAGA 
GATATCTTTA AGTTGATCTT GCTTGAGCCG GTTCACTACT GGCACTCGTT ACTTTTCATG 
GACCAGCATA ACTACGCTGG TATCCGAGCT ACCGGAGATG AAATTTCAGG TAACGAAGTT 
ACTATTGAAC CAAAGTGGAG CGTTGCACGG CATTTACCCG AGTATATTCA GAAGGATTTT 
ATCATAATTG TTGCCACGTT TATATTTGGC CCCTGGAAAT TTGCATTAGA GAAAAAAAGG 
GGCAGTGCAG AGAGTTTAGA GGCAGAGATG GTAGAATATC TCAAGGAACA GATAGGAACC 
CGGTTCATAA GCATGATTGT TGAGAAGATT GGTAATATCA GGTCACACAT CAAGGATATA 
AACGTGTCAG ATGCATCTTG GGCTTCTGGT CAAGCTCCCA AAGGAGATTA CACGTTCGAA 
TTTATACAAA TTATTACTGC TGTTCTGGCT CTTGATCAAA ACGTTCAGCA AGATGTTCTG 
GTAATGAGAA AGATTCTGTT GAAGTACATT AAAGTAAAAG AATGTGCTGC TGAAGCCGAA 
TTTATTGATC CTGGGCCATC CTTCATCTTA CCCAACGTGG CTTGCAGCAA CTGCGGTGCC 
TACAGAGACC TAGATTTCTG CAGAGACTCA GCTCTCTTAA CAGAAAAAGA ATGGTCATGT 
GCGGATCCGC AATGTGTGAA GATATATGAC AAAGAGCAGA TAGAGAGTAG CATTATCCAG 
ATGGTGAGAC AGAGGGAAAG AATGTACCAG TTGCAGGATC TAGTGTGCAA TAGGTGCAAT 
CAGGTGAAAG CCGCTCATTT GACAGAGCAG TGTGAGTGTT CTGGATCCTT TAGATGCAAG 
GAGAGTGGTT CAGATTTCCA CAAGAGGATT GAGATTTTCT TGGATATAGC AAAGCGCCAG 
AAGTTTAGAC TGCTAGAAGA ATGCATCTCC TGGATTTTAT TTGCCACCAG CTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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TIL2 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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