Pja2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pja2
Entrez Gene ID	224938
Full Name	praja ring finger ubiquitin ligase 2
Synonyms	AI447901,AL022700,Neurodap1,mKIAA0438
General protein information	Preferred Names praja ring finger ubiquitin ligase 2 Names E3 ubiquitin-protein ligase Praja-2 E3 ubiquitin-protein ligase Praja2 RING finger protein 131 RING-type E3 ubiquitin transferase Praja-2 neurodegeneration associated protein 1 praja 2, RING-H2 motif containing praja-2 praja2 protein carrying the RING-H2 sequence motif
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E1.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001025309.1	NP_001020480.1	E3 ubiquitin-protein ligase Praja-2 isoform a
NM_144859.2	NP_659108.1	E3 ubiquitin-protein ligase Praja-2 isoform b

Pathways from BioSystems

Homologies

Homo sapiens (human)	PJA2	NP_055634.3
Pan troglodytes (chimpanzee)	PJA2	XP_526975.2
	PJA2	XP_001100134.1
Canis lupus familiaris (dog)	PJA2	XP_536288.3
Bos taurus (cattle)	PJA2	NP_001093810.1
Rattus norvegicus (Norway rat)	Pja2	NP_620251.1
Xenopus tropicalis (tropical clawed frog)	pja2	XP_004910519.1
Gallus gallus (chicken)	PJA2	XP_413980.3
Danio rerio (zebrafish)	pja2	XP_005165620.1
Danio rerio (zebrafish)	LOC101882388	XP_005165623.1
Mus musculus (house mouse)	Pja2	NP_001020480.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

praja2 regulates KSR1 stability and mitogenic signaling.
Rinaldi L, Delle Donne R, Sepe M, Porpora M, Garbi C, Chiuso F, Gallo A, Parisi S, Russo L, Bachmann V, Huber RG, Stefan E, Russo T, Feliciello A
Cell death & disease7e2230(2016 May)

SRSF10 Plays a Role in Myoblast Differentiation and Glucose Production via Regulation of Alternative Splicing.
Wei N, Cheng Y, Wang Z, Liu Y, Luo C, Liu L, Chen L, Xie Z, Lu Y, Feng Y
Cell reports13(8)1647-57(2015 Nov)

Proteolytic control of neurite outgrowth inhibitor NOGO-A by the cAMP/PKA pathway.
Sepe M, Lignitto L, Porpora M, Delle Donne R, Rinaldi L, Belgianni G, Colucci G, Cuomo O, Viggiano D, Scorziello A, Garbi C, Annunziato L, Feliciello A
Proceedings of the National Academy of Sciences of the United States of America111(44)15729-34(2014 Nov)

Role of the SIK2-p35-PJA2 complex in pancreatic ?-cell functional compensation.
Sakamaki J, Fu A, Reeks C, Baird S, Depatie C, Al Azzabi M, Bardeesy N, Gingras AC, Yee SP, Screaton RA
Nature cell biology16(3)234-44(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

praja2 regulates KSR1 stability and mitogenic signaling.
Title: praja2 regulates KSR1 stability and mitogenic signaling.

The SIK2-p35-PJA2 complex is essential for glucose homeostasis and provides a link between p35-CDK5 and the AMPK family in excitable cells.
Title: Role of the SIK2-p35-PJA2 complex in pancreatic ?-cell functional compensation.

The following Pja2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pja2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22215	NM_144859.2 Latest version!	Mus musculus praja ring finger ubiquitin ligase 2 (Pja2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu22274	NM_001025309.1 Latest version!	Mus musculus praja ring finger ubiquitin ligase 2 (Pja2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$419.30 $599.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pja2 ( NM_144859.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_659108.1 Mus musculus praja ring finger ubiquitin ligase 2 (Pja2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22215
Clone ID Related Accession (Same CDS sequence)	NM_144859.2
Accession Version	NM_144859.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase Praja-2 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY065517.1, BC017130.1, BX518494.1, AA067149.1 and AK122282.1. On Jul 13, 2005 this sequence version replaced NM_144859.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (b) lacks an internal segment, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: SRR1660819.189956.1, SRR1660821.80842.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGTCACAGT ACACTGAAAA AGAGCCATCA GTAATGGATC AAGAATCCAG CAAGGCTGCT 
TGGCCTAAAC CAGCAGGAGG ATATCAGACT ATCACAGGCA GGAGATATGG AAGAAGACAT 
GCGTATGTCA GTTTTAAGCC ATGTATGACC AGGCATGAAA GAAGCTTGGG TCGGGCTGGT 
GATGACTATG AAGTGTTGGA ACTAGACGAT GTTCCAAAGG AGAACACCTC AGGTTCCAGT 
TCATTGGATC AAGTCCATCC TGCTTTACCA AATGAACCTA CAGTTGAAAA AAGTGAAACA 
GAAATTTCTA CTTGTGGTCC AGCACTGAAT CAAAGCACTG AGAGCAGTCC ATCCATTGCC 
ACAGTATGTC ATAGTGAGGA AGTCAGGGAG ACCTTAGAAA GCAATACAAA CCTTCATAAT 
CGCACTGAGA CAGAGCATAC TCCAGCAGTT TGTAATGTCT CAAGTGTCCA GAATGGAATT 
ATGTTGGTTC ATACTGACTC TTATGATCCA GACAGCAAGC ATGATGAGAA TGGCTCTCTT 
CAACTTGGCG CTGAAGCTGT GGAAGGTGGT AGACATCAGA AGGGGTTAGG CCGTGCAGTC 
TTTGAGCTGG AAAATGGAGA GGCAGAGATC TACGCTGACC TGTCACCATC AGTCCCCTCT 
CTCAACGGTG AAATAAGTGA GGCGTTTGAA GAGCTAGATT CAGCACCTTT AGAGAAAAGT 
TCTACTGCCG ATGCTGAGCT TGTCCATCAG AATGGGCAGG AATTTCAGAG GTCTTCTGAA 
GATGGCGTTG TTAGAAAGAG GCGACAAGAC GATACTGATC AGGGAAGACA GACAGAAAAT 
TCAACTGAAG ATGCAGACTG TGCTCCAGGC CATGTTGAGC AAAATACTAG TGATAGAGCC 
AACCACCATG GAAGTTCTCC TGAACAGGTA GTAAGGCCCA AAGTTAGAAA AGTGATAAGT 
TCAAGCCAGG TGGACCAAGA AATTGGTTTT AATAGGCACG AGGCTAAGCA GAGAAGCGTT 
CAGAGATGGA GAGAGGCTTT GGAAGTGGAG GAGTGCAGTT CAGATGACCC TATAATAAAG 
TGTGACGATT ATGATGGAGA CCACGACTGC ATGTTCCTAA CTCCAACTTA CTCAAGAGTT 
ACACAAAGGG AGACAGAACG GAATCGTGTA ACATCAGAAA ATGGAGCTAC AGCTTCAGGA 
CGGCAAGAAT CTCGGGACAA TGCCTTTTGG AATGCCTGCG GAGAGTATTA CCAACTCTTT 
GACAAAGATG AAGACAGGGA ACAGACATCC TTGGAAGAGG GAGAGATCCC TTGGTTACAG 
TACAATGAGG TCAATGAGAG CAGCAGCGAT GAAGGGAATG AGCCTGCCAA TGAGTTTGCA 
CAGCCGGAAG CTTTCATGTT GGATGGGAAC AACAACCTTG AGGACGACTC GAGCGTGAGT 
GAAGACTTGG ATGTGGACTG GAGCCTGTTT GATGGTTTTG CAGACGGACT TGGAGTTGCT 
GAAGCTATTT CTTATGTGGA TCCTCAGTTC CTTACCTACA TGGCACTAGA AGAGCGCTTA 
GCCCAGGCTA TGGAGACTGC GCTGGCACAT TTAGAGTCCC TTGCAGTAGA CGTTGAGGTG 
GCCAATCCAC CTGCCAGTAA GGAAAGCATT GATGGGCTTC CAGAGACTCT GGTTCTAGAG 
GACCACACCG CCATCGGCCA GGAGCAGTGC TGTCCCATCT GCTGCAGCGA GTACATCAAG 
GATGACATTG CCACAGAGCT GCCTTGCCAT CACTTCTTTC ATAAGCCCTG TGTCTCCATC 
TGGCTACAGA AGTCCGGAAC ATGCCCTGTG TGCCGCCGTC ACTTCCCACC TGCAGTTATT 
GACGCATCTG CAGCTGCTTC CTCTGACCCT GACCCTGATG CCTCGCCTGC AAATGACAAT 
GCTGAAGAAG CCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659108.1
CDS	176..2113
Translation	MSQYTEKEPSVMDQESSKAAWPKPAGGYQTITGRRYGRRHAYVSFKPCMTRHERSLGRAGDDYEVLELDDVPKENTSGSSSLDQVHPALPNEPTVEKSETEISTCGPALNQSTESSPSIATVCHSEEVRETLESNTNLHNRTETEHTPAVCNVSSVQNGIMLVHTDSYDPDSKHDENGSLQLGAEAVEGGRHQKGLGRAVFELENGEAEIYADLSPSVPSLNGEISEAFEELDSAPLEKSSTADAELVHQNGQEFQRSSEDGVVRKRRQDDTDQGRQTENSTEDADCAPGHVEQNTSDRANHHGSSPEQVVRPKVRKVISSSQVDQEIGFNRHEAKQRSVQRWREALEVEECSSDDPIIKCDDYDGDHDCMFLTPTYSRVTQRETERNRVTSENGATASGRQESRDNAFWNACGEYYQLFDKDEDREQTSLEEGEIPWLQYNEVNESSSDEGNEPANEFAQPEAFMLDGNNNLEDDSSVSEDLDVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLEDHTAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVIDASAAASSDPDPDASPANDNAEEAP

Target ORF information:

RefSeq Version	NM_144859.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus praja ring finger ubiquitin ligase 2 (Pja2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144859.2

ORF Insert Sequence:

ATGTCACAGT ACACTGAAAA AGAGCCATCA GTAATGGATC AAGAATCCAG CAAGGCTGCT 
TGGCCTAAAC CAGCAGGAGG ATATCAGACT ATCACAGGCA GGAGATATGG AAGAAGACAT 
GCGTATGTCA GTTTTAAGCC ATGTATGACC AGGCATGAAA GAAGCTTGGG TCGGGCTGGT 
GATGACTATG AAGTGTTGGA ACTAGACGAT GTTCCAAAGG AGAACACCTC AGGTTCCAGT 
TCATTGGATC AAGTCCATCC TGCTTTACCA AATGAACCTA CAGTTGAAAA AAGTGAAACA 
GAAATTTCTA CTTGTGGTCC AGCACTGAAT CAAAGCACTG AGAGCAGTCC ATCCATTGCC 
ACAGTATGTC ATAGTGAGGA AGTCAGGGAG ACCTTAGAAA GCAATACAAA CCTTCATAAT 
CGCACTGAGA CAGAGCATAC TCCAGCAGTT TGTAATGTCT CAAGTGTCCA GAATGGAATT 
ATGTTGGTTC ATACTGACTC TTATGATCCA GACAGCAAGC ATGATGAGAA TGGCTCTCTT 
CAACTTGGCG CTGAAGCTGT GGAAGGTGGT AGACATCAGA AGGGGTTAGG CCGTGCAGTC 
TTTGAGCTGG AAAATGGAGA GGCAGAGATC TACGCTGACC TGTCACCATC AGTCCCCTCT 
CTCAACGGTG AAATAAGTGA GGCGTTTGAA GAGCTAGATT CAGCACCTTT AGAGAAAAGT 
TCTACTGCCG ATGCTGAGCT TGTCCATCAG AATGGGCAGG AATTTCAGAG GTCTTCTGAA 
GATGGCGTTG TTAGAAAGAG GCGACAAGAC GATACTGATC AGGGAAGACA GACAGAAAAT 
TCAACTGAAG ATGCAGACTG TGCTCCAGGC CATGTTGAGC AAAATACTAG TGATAGAGCC 
AACCACCATG GAAGTTCTCC TGAACAGGTA GTAAGGCCCA AAGTTAGAAA AGTGATAAGT 
TCAAGCCAGG TGGACCAAGA AATTGGTTTT AATAGGCACG AGGCTAAGCA GAGAAGCGTT 
CAGAGATGGA GAGAGGCTTT GGAAGTGGAG GAGTGCAGTT CAGATGACCC TATAATAAAG 
TGTGACGATT ATGATGGAGA CCACGACTGC ATGTTCCTAA CTCCAACTTA CTCAAGAGTT 
ACACAAAGGG AGACAGAACG GAATCGTGTA ACATCAGAAA ATGGAGCTAC AGCTTCAGGA 
CGGCAAGAAT CTCGGGACAA TGCCTTTTGG AATGCCTGCG GAGAGTATTA CCAACTCTTT 
GACAAAGATG AAGACAGGGA ACAGACATCC TTGGAAGAGG GAGAGATCCC TTGGTTACAG 
TACAATGAGG TCAATGAGAG CAGCAGCGAT GAAGGGAATG AGCCTGCCAA TGAGTTTGCA 
CAGCCGGAAG CTTTCATGTT GGATGGGAAC AACAACCTTG AGGACGACTC GAGCGTGAGT 
GAAGACTTGG ATGTGGACTG GAGCCTGTTT GATGGTTTTG CAGACGGACT TGGAGTTGCT 
GAAGCTATTT CTTATGTGGA TCCTCAGTTC CTTACCTACA TGGCACTAGA AGAGCGCTTA 
GCCCAGGCTA TGGAGACTGC GCTGGCACAT TTAGAGTCCC TTGCAGTAGA CGTTGAGGTG 
GCCAATCCAC CTGCCAGTAA GGAAAGCATT GATGGGCTTC CAGAGACTCT GGTTCTAGAG 
GACCACACCG CCATCGGCCA GGAGCAGTGC TGTCCCATCT GCTGCAGCGA GTACATCAAG 
GATGACATTG CCACAGAGCT GCCTTGCCAT CACTTCTTTC ATAAGCCCTG TGTCTCCATC 
TGGCTACAGA AGTCCGGAAC ATGCCCTGTG TGCCGCCGTC ACTTCCCACC TGCAGTTATT 
GACGCATCTG CAGCTGCTTC CTCTGACCCT GACCCTGATG CCTCGCCTGC AAATGACAAT 
GCTGAAGAAG CCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pja2 ( NM_001025309.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001020480.1 Mus musculus praja ring finger ubiquitin ligase 2 (Pja2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22274
Clone ID Related Accession (Same CDS sequence)	NM_001025309.1
Accession Version	NM_001025309.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase Praja-2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY065517.1 and AK122282.1. Transcript Variant: This variant (1) encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: AK122282.1, SRR1660819.35498.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END##

ATGTCACAGT ACACTGAAAA AGAGCCATCA GTAATGGATC AAGAATCCAG CAAGGCTGCT 
TGGCCTAAAC CAGCAGGAGG ATATCAGACT ATCACAGGCA GGAGATATGG AAGAAGACAT 
GCGTATGTCA GTTTTAAGCC ATGTATGACC AGGCATGAAA GAAGCTTGGG TCGGGCTGGT 
GATGACTATG AAGTGTTGGA ACTAGACGAT GTTCCAAAGG AGAACACCTC AGGTTCCAGT 
TCATTGGATC AAGTCCATCC TGCTTTACCA AATGAACCTA CAGTTGAAAA AAGTGAAACA 
GAAATTTCTA CTTGTGGTCC AGCACTGAAT CAAAGCACTG AGAGCAGTCC ATCCATTGCC 
ACAGTATGTC ATAGTGAGGA AGTCAGGGAG ACCTTAGAAA GCAATACAAA CCTTCATAAT 
CGCACTGAGA CAGAGCATAC TCCAGCAGTT TGTAATGTCT CAAGTGTCCA GAATGGAATT 
ATGTTGGTTC ATACTGACTC TTATGATCCA GACAGCAAGC ATGATGAGAA TGGCTCTCTT 
CAACTTGGCG CTGAAGCTGT GGAAGGTGGT AGACATCAGA AGGGGTTAGG CCGTGCAGTC 
TTTGAGCTGG AAAATGGAGA GGCAGAGATC TACGCTGACC TGTCACCATC AGTCCCCTCT 
CTCAACGGTG AAATAAGTGA GGCGTTTGAA GAGCTAGATT CAGCACCTTT AGAGAAAAGT 
TCTACTGCCG ATGCTGAGCT TGTCCATCAG AATGGGCAGG AATTTCAGAG GTCTTCTGAA 
GATGGCGTTG TTAGAAAGAG GCGACAAGAC GATACTGATC AGGGAAGACA GACAGAAAAT 
TCAACTGAAG ATGCAGACTG TGCTCCAGGC CATGTTGAGC AAAATACTAG TGATAGAGCC 
AACCACCATG GAAGTTCTCC TGAACAGGTA GTAAGGCCCA AAGTTAGAAA AGTGATAAGT 
TCAAGCCAGG TGGACCAAGA AATTGGTTTT AATAGGCACG AGGCTAAGCA GAGAAGCGTT 
CAGAGATGGA GAGAGGCTTT GGAAGTGGAG GAGTGCAGTT CAGATGACCC TATAATAAAG 
TGTGACGATT ATGATGGAGA CCACGACTGC ATGTTCCTAA CTCCAACTTA CTCAAGAGTT 
ACACAAAGGG AGACAGAACG GAATCGTGTA ACATCAGAAA ATGGAGCTAC AGCTTCAGGA 
CGGCAAGAAT CTCGGGACAA TGCCTTTTGG AATGCCTGCG GAGAGTATTA CCAACTCTTT 
GACAAAGATG AAGACAGTTC AGAGTGCAGT GATGGGGAAT GGTCTGCTTC TCTGCCTCAC 
CGATTTTCTG GCACAGAAAA AGACCAGTCC TCAAGCGATG ACAGCTGGGA AACTCTGCCA 
GGAAAGGATG AGAATGACCC TGAGCTGCAG AGTGATAGTA GTGGCCCCGA GGAAGAAAAC 
CAAGAGTTGT CTCTTCAGGA GGGGGAACAG ACATCCTTGG AAGAGGGAGA GATCCCTTGG 
TTACAGTACA ATGAGGTCAA TGAGAGCAGC AGCGATGAAG GGAATGAGCC TGCCAATGAG 
TTTGCACAGC CGGAAGCTTT CATGTTGGAT GGGAACAACA ACCTTGAGGA CGACTCGAGC 
GTGAGTGAAG ACTTGGATGT GGACTGGAGC CTGTTTGATG GTTTTGCAGA CGGACTTGGA 
GTTGCTGAAG CTATTTCTTA TGTGGATCCT CAGTTCCTTA CCTACATGGC ACTAGAAGAG 
CGCTTAGCCC AGGCTATGGA GACTGCGCTG GCACATTTAG AGTCCCTTGC AGTAGACGTT 
GAGGTGGCCA ATCCACCTGC CAGTAAGGAA AGCATTGATG GGCTTCCAGA GACTCTGGTT 
CTAGAGGACC ACACCGCCAT CGGCCAGGAG CAGTGCTGTC CCATCTGCTG CAGCGAGTAC 
ATCAAGGATG ACATTGCCAC AGAGCTGCCT TGCCATCACT TCTTTCATAA GCCCTGTGTC 
TCCATCTGGC TACAGAAGTC CGGAACATGC CCTGTGTGCC GCCGTCACTT CCCACCTGCA 
GTTATTGACG CATCTGCAGC TGCTTCCTCT GACCCTGACC CTGATGCCTC GCCTGCAAAT 
GACAATGCTG AAGAAGCCCC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020480.1
CDS	176..2299
Translation	MSQYTEKEPSVMDQESSKAAWPKPAGGYQTITGRRYGRRHAYVSFKPCMTRHERSLGRAGDDYEVLELDDVPKENTSGSSSLDQVHPALPNEPTVEKSETEISTCGPALNQSTESSPSIATVCHSEEVRETLESNTNLHNRTETEHTPAVCNVSSVQNGIMLVHTDSYDPDSKHDENGSLQLGAEAVEGGRHQKGLGRAVFELENGEAEIYADLSPSVPSLNGEISEAFEELDSAPLEKSSTADAELVHQNGQEFQRSSEDGVVRKRRQDDTDQGRQTENSTEDADCAPGHVEQNTSDRANHHGSSPEQVVRPKVRKVISSSQVDQEIGFNRHEAKQRSVQRWREALEVEECSSDDPIIKCDDYDGDHDCMFLTPTYSRVTQRETERNRVTSENGATASGRQESRDNAFWNACGEYYQLFDKDEDSSECSDGEWSASLPHRFSGTEKDQSSSDDSWETLPGKDENDPELQSDSSGPEEENQELSLQEGEQTSLEEGEIPWLQYNEVNESSSDEGNEPANEFAQPEAFMLDGNNNLEDDSSVSEDLDVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLEDHTAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVIDASAAASSDPDPDASPANDNAEEAP

Target ORF information:

RefSeq Version	NM_001025309.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus praja ring finger ubiquitin ligase 2 (Pja2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025309.1

ORF Insert Sequence:

ATGTCACAGT ACACTGAAAA AGAGCCATCA GTAATGGATC AAGAATCCAG CAAGGCTGCT 
TGGCCTAAAC CAGCAGGAGG ATATCAGACT ATCACAGGCA GGAGATATGG AAGAAGACAT 
GCGTATGTCA GTTTTAAGCC ATGTATGACC AGGCATGAAA GAAGCTTGGG TCGGGCTGGT 
GATGACTATG AAGTGTTGGA ACTAGACGAT GTTCCAAAGG AGAACACCTC AGGTTCCAGT 
TCATTGGATC AAGTCCATCC TGCTTTACCA AATGAACCTA CAGTTGAAAA AAGTGAAACA 
GAAATTTCTA CTTGTGGTCC AGCACTGAAT CAAAGCACTG AGAGCAGTCC ATCCATTGCC 
ACAGTATGTC ATAGTGAGGA AGTCAGGGAG ACCTTAGAAA GCAATACAAA CCTTCATAAT 
CGCACTGAGA CAGAGCATAC TCCAGCAGTT TGTAATGTCT CAAGTGTCCA GAATGGAATT 
ATGTTGGTTC ATACTGACTC TTATGATCCA GACAGCAAGC ATGATGAGAA TGGCTCTCTT 
CAACTTGGCG CTGAAGCTGT GGAAGGTGGT AGACATCAGA AGGGGTTAGG CCGTGCAGTC 
TTTGAGCTGG AAAATGGAGA GGCAGAGATC TACGCTGACC TGTCACCATC AGTCCCCTCT 
CTCAACGGTG AAATAAGTGA GGCGTTTGAA GAGCTAGATT CAGCACCTTT AGAGAAAAGT 
TCTACTGCCG ATGCTGAGCT TGTCCATCAG AATGGGCAGG AATTTCAGAG GTCTTCTGAA 
GATGGCGTTG TTAGAAAGAG GCGACAAGAC GATACTGATC AGGGAAGACA GACAGAAAAT 
TCAACTGAAG ATGCAGACTG TGCTCCAGGC CATGTTGAGC AAAATACTAG TGATAGAGCC 
AACCACCATG GAAGTTCTCC TGAACAGGTA GTAAGGCCCA AAGTTAGAAA AGTGATAAGT 
TCAAGCCAGG TGGACCAAGA AATTGGTTTT AATAGGCACG AGGCTAAGCA GAGAAGCGTT 
CAGAGATGGA GAGAGGCTTT GGAAGTGGAG GAGTGCAGTT CAGATGACCC TATAATAAAG 
TGTGACGATT ATGATGGAGA CCACGACTGC ATGTTCCTAA CTCCAACTTA CTCAAGAGTT 
ACACAAAGGG AGACAGAACG GAATCGTGTA ACATCAGAAA ATGGAGCTAC AGCTTCAGGA 
CGGCAAGAAT CTCGGGACAA TGCCTTTTGG AATGCCTGCG GAGAGTATTA CCAACTCTTT 
GACAAAGATG AAGACAGTTC AGAGTGCAGT GATGGGGAAT GGTCTGCTTC TCTGCCTCAC 
CGATTTTCTG GCACAGAAAA AGACCAGTCC TCAAGCGATG ACAGCTGGGA AACTCTGCCA 
GGAAAGGATG AGAATGACCC TGAGCTGCAG AGTGATAGTA GTGGCCCCGA GGAAGAAAAC 
CAAGAGTTGT CTCTTCAGGA GGGGGAACAG ACATCCTTGG AAGAGGGAGA GATCCCTTGG 
TTACAGTACA ATGAGGTCAA TGAGAGCAGC AGCGATGAAG GGAATGAGCC TGCCAATGAG 
TTTGCACAGC CGGAAGCTTT CATGTTGGAT GGGAACAACA ACCTTGAGGA CGACTCGAGC 
GTGAGTGAAG ACTTGGATGT GGACTGGAGC CTGTTTGATG GTTTTGCAGA CGGACTTGGA 
GTTGCTGAAG CTATTTCTTA TGTGGATCCT CAGTTCCTTA CCTACATGGC ACTAGAAGAG 
CGCTTAGCCC AGGCTATGGA GACTGCGCTG GCACATTTAG AGTCCCTTGC AGTAGACGTT 
GAGGTGGCCA ATCCACCTGC CAGTAAGGAA AGCATTGATG GGCTTCCAGA GACTCTGGTT 
CTAGAGGACC ACACCGCCAT CGGCCAGGAG CAGTGCTGTC CCATCTGCTG CAGCGAGTAC 
ATCAAGGATG ACATTGCCAC AGAGCTGCCT TGCCATCACT TCTTTCATAA GCCCTGTGTC 
TCCATCTGGC TACAGAAGTC CGGAACATGC CCTGTGTGCC GCCGTCACTT CCCACCTGCA 
GTTATTGACG CATCTGCAGC TGCTTCCTCT GACCCTGACC CTGATGCCTC GCCTGCAAAT 
GACAATGCTG AAGAAGCCCC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

praja2 regulates KSR1 stability and mitogenic signaling.
Cell death & disease7e2230(2016 May)
Rinaldi L,Delle Donne R,Sepe M,Porpora M,Garbi C,Chiuso F,Gallo A,Parisi S,Russo L,Bachmann V,Huber RG,Stefan E,Russo T,Feliciello A

SRSF10 Plays a Role in Myoblast Differentiation and Glucose Production via Regulation of Alternative Splicing.
Cell reports13(8)1647-57(2015 Nov)
Wei N,Cheng Y,Wang Z,Liu Y,Luo C,Liu L,Chen L,Xie Z,Lu Y,Feng Y

Proteolytic control of neurite outgrowth inhibitor NOGO-A by the cAMP/PKA pathway.
Proceedings of the National Academy of Sciences of the United States of America111(44)15729-34(2014 Nov)
Sepe M,Lignitto L,Porpora M,Delle Donne R,Rinaldi L,Belgianni G,Colucci G,Cuomo O,Viggiano D,Scorziello A,Garbi C,Annunziato L,Feliciello A

Role of the SIK2-p35-PJA2 complex in pancreatic ?-cell functional compensation.
Nature cell biology16(3)234-44(2014 Mar)
Sakamaki J,Fu A,Reeks C,Baird S,Depatie C,Al Azzabi M,Bardeesy N,Gingras AC,Yee SP,Screaton RA

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Pja2 cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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