Pja2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pja2
Entrez Gene ID	192256
Full Name	praja ring finger ubiquitin ligase 2
Synonyms	Neurodap1,praja2
General protein information	Preferred Names praja ring finger ubiquitin ligase 2 Names E3 ubiquitin-protein ligase Praja-2 RING-type E3 ubiquitin transferase Praja-2 praja 2, RING-H2 motif containing praja ring finger 2, E3 ubiquitin protein ligase protein carrying the RING-H2 sequence motif
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q37
Summary	may play a role in synaptic communication and plasticity through the control of the formation of post synaptic density [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138896.2	NP_620251.1	E3 ubiquitin-protein ligase Praja-2 isoform 1
NM_001277278.1	NP_001264207.1	E3 ubiquitin-protein ligase Praja-2 isoform 2
XM_017596266.1	XP_017451755.1	E3 ubiquitin-protein ligase Praja-2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PJA2	NP_055634.3
Pan troglodytes (chimpanzee)	PJA2	XP_526975.2
	PJA2	XP_001100134.1
Canis lupus familiaris (dog)	PJA2	XP_536288.3
Bos taurus (cattle)	PJA2	NP_001093810.1
Rattus norvegicus (Norway rat)	Pja2	NP_620251.1
Xenopus tropicalis (tropical clawed frog)	pja2	XP_004910519.1
Gallus gallus (chicken)	PJA2	XP_413980.3
Danio rerio (zebrafish)	pja2	XP_005165620.1
Danio rerio (zebrafish)	LOC101882388	XP_005165623.1
Mus musculus (house mouse)	Pja2	NP_001020480.1

Gene Ontology
Bibliography

Related articles in PubMed

Ubiquitylation of MFHAS1 by the ubiquitin ligase praja2 promotes M1 macrophage polarization by activating JNK and p38 pathways.
Zhong J, Wang H, Chen W, Sun Z, Chen J, Xu Y, Weng M, Shi Q, Ma D, Miao C
Cell death & disease8(5)e2763(2017 May)

Control of PKA stability and signalling by the RING ligase praja2.
Lignitto L, Carlucci A, Sepe M, Stefan E, Cuomo O, Nistic? R, Scorziello A, Savoia C, Garbi C, Annunziato L, Feliciello A
Nature cell biology13(4)412-22(2011 Apr)

A novel RING-H2 motif protein downregulated by axotomy: its characteristic localization at the postsynaptic density of axosomatic synapse.
Nakayama M, Miyake T, Gahara Y, Ohara O, Kitamura T
The Journal of neuroscience : the official journal of the Society for Neuroscience15(7 Pt 2)5238-48(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that praja2 regulates the stability and total concentration of protein kinase A R subunits, tuning the strength and duration of PKA signal output in response to cAMP.
Title: Control of PKA stability and signalling by the RING ligase praja2.

The following Pja2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pja2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01928	NM_138896.2 Latest version!	Rattus norvegicus praja ring finger ubiquitin ligase 2 (Pja2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$419.30 $599.00
ORa01928	XM_017596266.1 Latest version!	Rattus norvegicus praja ring finger ubiquitin ligase 2 (Pja2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$419.30 $599.00
ORa11812	NM_001277278.1 Latest version!	Rattus norvegicus praja ring finger ubiquitin ligase 2 (Pja2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pja2 ( NM_138896.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620251.1 Rattus norvegicus praja ring finger ubiquitin ligase 2 (Pja2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa01928
Clone ID Related Accession (Same CDS sequence)	NM_138896.2 , XM_017596266.1
Accession Version	NM_138896.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase Praja-2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB577390.1, BC074015.1 and AABR07068612.1. On Mar 28, 2013 this sequence version replaced NM_138896.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC074015.1, D32249.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCACAGT ATACAGAAAA AGAACCATCA GTAATGGATC AAGACTCCAG CAAGGCTGCG 
TGGCCTAGAG CAGCAGGAGG ATACCAGACG ATTACAGGCA GGAGGTACGG AAGGAGACAT 
GCGTATGTCA GTTTTAAGCC ATGTATGACC AGGCATGAAC GGAGCTTGGG TCGGGCTGGC 
GATGACTATG AAGTTCTGGA ACTGGATGAC GTTGCCAAGG AAAACACCGC AGGTTCCAGT 
TCATTGGATC AAGTCCATCC TTCTTTACCC AGTGAAACTA CAGTTGAAAA AAGTGAAACA 
GAAATTCCTA CTTGTGGTCC AGCACTGAAT CAAAGCACGG AGAGCAACCC ATCCGTTGCC 
ACAGTGTGTC ATAGTGAGGA AGTCAGGGAG ACCTTAGACA GCAGTACGAA TCTTCAGAAT 
CACGCTGAGA GAGAGTGTAC GCCAGCAGTT TGTAATGCCT CAAGTGTCCA GAATGGAATT 
GTGTTGGTTC ATACTGACTC TTATGATCCA GACAGCAAGC ATGATGAGAA TGACTCTCTT 
CAACTTTGTG CCCAAGCTGT GGAAGGTGGT AGACGTCAGA AGGTATTAGG CAATGCAGTC 
TTTGAGCTGG AAAATGGAGA GGTAGAGAGA TATGCTGATC TGTGTCCCTC AGTTCCCTCT 
CTCAGTGGTG AAATAAGGGA GGAGTCTGAA GAGCTAGGTT CAGCACTCTT AGAGAAAAAT 
TCTGCTGGCG ATGCGGAGGC TGTCCATCAG GATGGGCAGG AATTTCAGAG GTCTTCTGAA 
GACGGCATTG TTAGAAAGAG GCGACAAGAT GATACCGATC AGGGAAGACA GACAGAAAAT 
TCAACTGAAG ATGCAGACTG TGTTCCAGGC CATGTTGAAC AAAATACTAG TGAGAGAGCC 
AATCACCATG GAAGTTCTCC TGAACAGGTA GTGAGGCCCA AAGTTAGAAA AGTGATCAGT 
TCAAGCCAGG TGGACCAAGA GAGCGGTTTT AATAGGCACG AGGCTAAGCA AAGAAGTGTT 
CAGAGGTGGA GAGAGGCTCT GGAAGTTGAG GAATGCAGTT CAGATGACCC TATAATCAAG 
TGTGACGATT ATGATGGAGA CCATGACTGC ATGTTCCTAA CCCCATCCTA CTCAAGAGTT 
ACGCCAAGGG AAGCAGAACG TCACCGTGCG ACAGCAGAAA ATGGAGCCAC AGCTTCAGGA 
AGGCAAGAGG CTCGGGAAAA TGCCTTTTGG AATGCCTGTG GAGAGTATTA CCAGCTCTTT 
GACAAAGACG AAGACAGTTC AGAGTGCAGT GATGGGGAAT GGTCTGCTTC TCTGCCTCAC 
CGATTTTCTG GCACAGAAAA AGACCAGTCC TCAAGCGATG AAAGCTGGGA AACTCTGCCA 
GGAAAAGATG AGAATGAACC TGAGCTACAG AGTGATAGTA GTGGCCCTGA GGAAGAAAAC 
CAAGAATTGT CTCTTCAGGA GGGAGAACAG ACGTCCTTGG AGGAGGGGGA GATTCCCTGG 
TTACAGTACA ATGAGGTCAA TGAGAGCAGC AGCGATGAAG GGAACGAGCC TGCCAATGAG 
TTTGCACAAC CAGAAGCTTT CATGTTGGAT GGGAACAACA ACCTGGAGGA CGACTCGAGC 
GTGAGTGAAG ACCTGGATGT GGACTGGAGC CTATTTGATG GTTTTGCGGA TGGACTTGGT 
GTTGCTGAAG CTATTTCCTA CGTGGATCCT CAGTTCCTCA CCTACATGGC ACTAGAAGAA 
CGCTTAGCCC AGGCTATGGA GACTGCACTG GCACACTTAG AGTCTCTTGC TGTAGACGTT 
GAGGTGGCTA ACCCACCTGC CAGTAAGGAA AGCATCGACG GACTTCCAGA AACCCTTGTC 
CTAGAAGATC ACACCGCTAT TGGTCAGGAG CAGTGCTGTC CCATCTGCTG CAGTGAGTAC 
ATTAAGGATG ACATCGCAAC AGAGCTGCCC TGCCATCACT TCTTTCATAA GCCTTGTGTC 
TCCATTTGGC TACAGAAGTC CGGAACGTGC CCTGTGTGCC GCCGCCACTT CCCACCTGCA 
GTGATTGACG CATCTGCAGC TGCTTCCTCT GAACCAGACC TTGATGCCTC GCCTGCAAAC 
GACAATGCTG AGGAAGCCCC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620251.1
CDS	205..2328
Translation	MSQYTEKEPSVMDQDSSKAAWPRAAGGYQTITGRRYGRRHAYVSFKPCMTRHERSLGRAGDDYEVLELDDVAKENTAGSSSLDQVHPSLPSETTVEKSETEIPTCGPALNQSTESNPSVATVCHSEEVRETLDSSTNLQNHAERECTPAVCNASSVQNGIVLVHTDSYDPDSKHDENDSLQLCAQAVEGGRRQKVLGNAVFELENGEVERYADLCPSVPSLSGEIREESEELGSALLEKNSAGDAEAVHQDGQEFQRSSEDGIVRKRRQDDTDQGRQTENSTEDADCVPGHVEQNTSERANHHGSSPEQVVRPKVRKVISSSQVDQESGFNRHEAKQRSVQRWREALEVEECSSDDPIIKCDDYDGDHDCMFLTPSYSRVTPREAERHRATAENGATASGRQEARENAFWNACGEYYQLFDKDEDSSECSDGEWSASLPHRFSGTEKDQSSSDESWETLPGKDENEPELQSDSSGPEEENQELSLQEGEQTSLEEGEIPWLQYNEVNESSSDEGNEPANEFAQPEAFMLDGNNNLEDDSSVSEDLDVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLEDHTAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVIDASAAASSEPDLDASPANDNAEEAP

Target ORF information:

RefSeq Version	NM_138896.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus praja ring finger ubiquitin ligase 2 (Pja2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138896.2

ORF Insert Sequence:

ATGTCACAGT ATACAGAAAA AGAACCATCA GTAATGGATC AAGACTCCAG CAAGGCTGCG 
TGGCCTAGAG CAGCAGGAGG ATACCAGACG ATTACAGGCA GGAGGTACGG AAGGAGACAT 
GCGTATGTCA GTTTTAAGCC ATGTATGACC AGGCATGAAC GGAGCTTGGG TCGGGCTGGC 
GATGACTATG AAGTTCTGGA ACTGGATGAC GTTGCCAAGG AAAACACCGC AGGTTCCAGT 
TCATTGGATC AAGTCCATCC TTCTTTACCC AGTGAAACTA CAGTTGAAAA AAGTGAAACA 
GAAATTCCTA CTTGTGGTCC AGCACTGAAT CAAAGCACGG AGAGCAACCC ATCCGTTGCC 
ACAGTGTGTC ATAGTGAGGA AGTCAGGGAG ACCTTAGACA GCAGTACGAA TCTTCAGAAT 
CACGCTGAGA GAGAGTGTAC GCCAGCAGTT TGTAATGCCT CAAGTGTCCA GAATGGAATT 
GTGTTGGTTC ATACTGACTC TTATGATCCA GACAGCAAGC ATGATGAGAA TGACTCTCTT 
CAACTTTGTG CCCAAGCTGT GGAAGGTGGT AGACGTCAGA AGGTATTAGG CAATGCAGTC 
TTTGAGCTGG AAAATGGAGA GGTAGAGAGA TATGCTGATC TGTGTCCCTC AGTTCCCTCT 
CTCAGTGGTG AAATAAGGGA GGAGTCTGAA GAGCTAGGTT CAGCACTCTT AGAGAAAAAT 
TCTGCTGGCG ATGCGGAGGC TGTCCATCAG GATGGGCAGG AATTTCAGAG GTCTTCTGAA 
GACGGCATTG TTAGAAAGAG GCGACAAGAT GATACCGATC AGGGAAGACA GACAGAAAAT 
TCAACTGAAG ATGCAGACTG TGTTCCAGGC CATGTTGAAC AAAATACTAG TGAGAGAGCC 
AATCACCATG GAAGTTCTCC TGAACAGGTA GTGAGGCCCA AAGTTAGAAA AGTGATCAGT 
TCAAGCCAGG TGGACCAAGA GAGCGGTTTT AATAGGCACG AGGCTAAGCA AAGAAGTGTT 
CAGAGGTGGA GAGAGGCTCT GGAAGTTGAG GAATGCAGTT CAGATGACCC TATAATCAAG 
TGTGACGATT ATGATGGAGA CCATGACTGC ATGTTCCTAA CCCCATCCTA CTCAAGAGTT 
ACGCCAAGGG AAGCAGAACG TCACCGTGCG ACAGCAGAAA ATGGAGCCAC AGCTTCAGGA 
AGGCAAGAGG CTCGGGAAAA TGCCTTTTGG AATGCCTGTG GAGAGTATTA CCAGCTCTTT 
GACAAAGACG AAGACAGTTC AGAGTGCAGT GATGGGGAAT GGTCTGCTTC TCTGCCTCAC 
CGATTTTCTG GCACAGAAAA AGACCAGTCC TCAAGCGATG AAAGCTGGGA AACTCTGCCA 
GGAAAAGATG AGAATGAACC TGAGCTACAG AGTGATAGTA GTGGCCCTGA GGAAGAAAAC 
CAAGAATTGT CTCTTCAGGA GGGAGAACAG ACGTCCTTGG AGGAGGGGGA GATTCCCTGG 
TTACAGTACA ATGAGGTCAA TGAGAGCAGC AGCGATGAAG GGAACGAGCC TGCCAATGAG 
TTTGCACAAC CAGAAGCTTT CATGTTGGAT GGGAACAACA ACCTGGAGGA CGACTCGAGC 
GTGAGTGAAG ACCTGGATGT GGACTGGAGC CTATTTGATG GTTTTGCGGA TGGACTTGGT 
GTTGCTGAAG CTATTTCCTA CGTGGATCCT CAGTTCCTCA CCTACATGGC ACTAGAAGAA 
CGCTTAGCCC AGGCTATGGA GACTGCACTG GCACACTTAG AGTCTCTTGC TGTAGACGTT 
GAGGTGGCTA ACCCACCTGC CAGTAAGGAA AGCATCGACG GACTTCCAGA AACCCTTGTC 
CTAGAAGATC ACACCGCTAT TGGTCAGGAG CAGTGCTGTC CCATCTGCTG CAGTGAGTAC 
ATTAAGGATG ACATCGCAAC AGAGCTGCCC TGCCATCACT TCTTTCATAA GCCTTGTGTC 
TCCATTTGGC TACAGAAGTC CGGAACGTGC CCTGTGTGCC GCCGCCACTT CCCACCTGCA 
GTGATTGACG CATCTGCAGC TGCTTCCTCT GAACCAGACC TTGATGCCTC GCCTGCAAAC 
GACAATGCTG AGGAAGCCCC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pja2 ( XM_017596266.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451755.1 Rattus norvegicus praja ring finger ubiquitin ligase 2 (Pja2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa01928
Clone ID Related Accession (Same CDS sequence)	NM_138896.2 , XM_017596266.1
Accession Version	XM_017596266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase Praja-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACAGT ATACAGAAAA AGAACCATCA GTAATGGATC AAGACTCCAG CAAGGCTGCG 
TGGCCTAGAG CAGCAGGAGG ATACCAGACG ATTACAGGCA GGAGGTACGG AAGGAGACAT 
GCGTATGTCA GTTTTAAGCC ATGTATGACC AGGCATGAAC GGAGCTTGGG TCGGGCTGGC 
GATGACTATG AAGTTCTGGA ACTGGATGAC GTTGCCAAGG AAAACACCGC AGGTTCCAGT 
TCATTGGATC AAGTCCATCC TTCTTTACCC AGTGAAACTA CAGTTGAAAA AAGTGAAACA 
GAAATTCCTA CTTGTGGTCC AGCACTGAAT CAAAGCACGG AGAGCAACCC ATCCGTTGCC 
ACAGTGTGTC ATAGTGAGGA AGTCAGGGAG ACCTTAGACA GCAGTACGAA TCTTCAGAAT 
CACGCTGAGA GAGAGTGTAC GCCAGCAGTT TGTAATGCCT CAAGTGTCCA GAATGGAATT 
GTGTTGGTTC ATACTGACTC TTATGATCCA GACAGCAAGC ATGATGAGAA TGACTCTCTT 
CAACTTTGTG CCCAAGCTGT GGAAGGTGGT AGACGTCAGA AGGTATTAGG CAATGCAGTC 
TTTGAGCTGG AAAATGGAGA GGTAGAGAGA TATGCTGATC TGTGTCCCTC AGTTCCCTCT 
CTCAGTGGTG AAATAAGGGA GGAGTCTGAA GAGCTAGGTT CAGCACTCTT AGAGAAAAAT 
TCTGCTGGCG ATGCGGAGGC TGTCCATCAG GATGGGCAGG AATTTCAGAG GTCTTCTGAA 
GACGGCATTG TTAGAAAGAG GCGACAAGAT GATACCGATC AGGGAAGACA GACAGAAAAT 
TCAACTGAAG ATGCAGACTG TGTTCCAGGC CATGTTGAAC AAAATACTAG TGAGAGAGCC 
AATCACCATG GAAGTTCTCC TGAACAGGTA GTGAGGCCCA AAGTTAGAAA AGTGATCAGT 
TCAAGCCAGG TGGACCAAGA GAGCGGTTTT AATAGGCACG AGGCTAAGCA AAGAAGTGTT 
CAGAGGTGGA GAGAGGCTCT GGAAGTTGAG GAATGCAGTT CAGATGACCC TATAATCAAG 
TGTGACGATT ATGATGGAGA CCATGACTGC ATGTTCCTAA CCCCATCCTA CTCAAGAGTT 
ACGCCAAGGG AAGCAGAACG TCACCGTGCG ACAGCAGAAA ATGGAGCCAC AGCTTCAGGA 
AGGCAAGAGG CTCGGGAAAA TGCCTTTTGG AATGCCTGTG GAGAGTATTA CCAGCTCTTT 
GACAAAGACG AAGACAGTTC AGAGTGCAGT GATGGGGAAT GGTCTGCTTC TCTGCCTCAC 
CGATTTTCTG GCACAGAAAA AGACCAGTCC TCAAGCGATG AAAGCTGGGA AACTCTGCCA 
GGAAAAGATG AGAATGAACC TGAGCTACAG AGTGATAGTA GTGGCCCTGA GGAAGAAAAC 
CAAGAATTGT CTCTTCAGGA GGGAGAACAG ACGTCCTTGG AGGAGGGGGA GATTCCCTGG 
TTACAGTACA ATGAGGTCAA TGAGAGCAGC AGCGATGAAG GGAACGAGCC TGCCAATGAG 
TTTGCACAAC CAGAAGCTTT CATGTTGGAT GGGAACAACA ACCTGGAGGA CGACTCGAGC 
GTGAGTGAAG ACCTGGATGT GGACTGGAGC CTATTTGATG GTTTTGCGGA TGGACTTGGT 
GTTGCTGAAG CTATTTCCTA CGTGGATCCT CAGTTCCTCA CCTACATGGC ACTAGAAGAA 
CGCTTAGCCC AGGCTATGGA GACTGCACTG GCACACTTAG AGTCTCTTGC TGTAGACGTT 
GAGGTGGCTA ACCCACCTGC CAGTAAGGAA AGCATCGACG GACTTCCAGA AACCCTTGTC 
CTAGAAGATC ACACCGCTAT TGGTCAGGAG CAGTGCTGTC CCATCTGCTG CAGTGAGTAC 
ATTAAGGATG ACATCGCAAC AGAGCTGCCC TGCCATCACT TCTTTCATAA GCCTTGTGTC 
TCCATTTGGC TACAGAAGTC CGGAACGTGC CCTGTGTGCC GCCGCCACTT CCCACCTGCA 
GTGATTGACG CATCTGCAGC TGCTTCCTCT GAACCAGACC TTGATGCCTC GCCTGCAAAC 
GACAATGCTG AGGAAGCCCC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451755.1
CDS	294..2417
Translation	MSQYTEKEPSVMDQDSSKAAWPRAAGGYQTITGRRYGRRHAYVSFKPCMTRHERSLGRAGDDYEVLELDDVAKENTAGSSSLDQVHPSLPSETTVEKSETEIPTCGPALNQSTESNPSVATVCHSEEVRETLDSSTNLQNHAERECTPAVCNASSVQNGIVLVHTDSYDPDSKHDENDSLQLCAQAVEGGRRQKVLGNAVFELENGEVERYADLCPSVPSLSGEIREESEELGSALLEKNSAGDAEAVHQDGQEFQRSSEDGIVRKRRQDDTDQGRQTENSTEDADCVPGHVEQNTSERANHHGSSPEQVVRPKVRKVISSSQVDQESGFNRHEAKQRSVQRWREALEVEECSSDDPIIKCDDYDGDHDCMFLTPSYSRVTPREAERHRATAENGATASGRQEARENAFWNACGEYYQLFDKDEDSSECSDGEWSASLPHRFSGTEKDQSSSDESWETLPGKDENEPELQSDSSGPEEENQELSLQEGEQTSLEEGEIPWLQYNEVNESSSDEGNEPANEFAQPEAFMLDGNNNLEDDSSVSEDLDVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLEDHTAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVIDASAAASSEPDLDASPANDNAEEAP

Target ORF information:

RefSeq Version	XM_017596266.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus praja ring finger ubiquitin ligase 2 (Pja2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596266.1

ORF Insert Sequence:

ATGTCACAGT ATACAGAAAA AGAACCATCA GTAATGGATC AAGACTCCAG CAAGGCTGCG 
TGGCCTAGAG CAGCAGGAGG ATACCAGACG ATTACAGGCA GGAGGTACGG AAGGAGACAT 
GCGTATGTCA GTTTTAAGCC ATGTATGACC AGGCATGAAC GGAGCTTGGG TCGGGCTGGC 
GATGACTATG AAGTTCTGGA ACTGGATGAC GTTGCCAAGG AAAACACCGC AGGTTCCAGT 
TCATTGGATC AAGTCCATCC TTCTTTACCC AGTGAAACTA CAGTTGAAAA AAGTGAAACA 
GAAATTCCTA CTTGTGGTCC AGCACTGAAT CAAAGCACGG AGAGCAACCC ATCCGTTGCC 
ACAGTGTGTC ATAGTGAGGA AGTCAGGGAG ACCTTAGACA GCAGTACGAA TCTTCAGAAT 
CACGCTGAGA GAGAGTGTAC GCCAGCAGTT TGTAATGCCT CAAGTGTCCA GAATGGAATT 
GTGTTGGTTC ATACTGACTC TTATGATCCA GACAGCAAGC ATGATGAGAA TGACTCTCTT 
CAACTTTGTG CCCAAGCTGT GGAAGGTGGT AGACGTCAGA AGGTATTAGG CAATGCAGTC 
TTTGAGCTGG AAAATGGAGA GGTAGAGAGA TATGCTGATC TGTGTCCCTC AGTTCCCTCT 
CTCAGTGGTG AAATAAGGGA GGAGTCTGAA GAGCTAGGTT CAGCACTCTT AGAGAAAAAT 
TCTGCTGGCG ATGCGGAGGC TGTCCATCAG GATGGGCAGG AATTTCAGAG GTCTTCTGAA 
GACGGCATTG TTAGAAAGAG GCGACAAGAT GATACCGATC AGGGAAGACA GACAGAAAAT 
TCAACTGAAG ATGCAGACTG TGTTCCAGGC CATGTTGAAC AAAATACTAG TGAGAGAGCC 
AATCACCATG GAAGTTCTCC TGAACAGGTA GTGAGGCCCA AAGTTAGAAA AGTGATCAGT 
TCAAGCCAGG TGGACCAAGA GAGCGGTTTT AATAGGCACG AGGCTAAGCA AAGAAGTGTT 
CAGAGGTGGA GAGAGGCTCT GGAAGTTGAG GAATGCAGTT CAGATGACCC TATAATCAAG 
TGTGACGATT ATGATGGAGA CCATGACTGC ATGTTCCTAA CCCCATCCTA CTCAAGAGTT 
ACGCCAAGGG AAGCAGAACG TCACCGTGCG ACAGCAGAAA ATGGAGCCAC AGCTTCAGGA 
AGGCAAGAGG CTCGGGAAAA TGCCTTTTGG AATGCCTGTG GAGAGTATTA CCAGCTCTTT 
GACAAAGACG AAGACAGTTC AGAGTGCAGT GATGGGGAAT GGTCTGCTTC TCTGCCTCAC 
CGATTTTCTG GCACAGAAAA AGACCAGTCC TCAAGCGATG AAAGCTGGGA AACTCTGCCA 
GGAAAAGATG AGAATGAACC TGAGCTACAG AGTGATAGTA GTGGCCCTGA GGAAGAAAAC 
CAAGAATTGT CTCTTCAGGA GGGAGAACAG ACGTCCTTGG AGGAGGGGGA GATTCCCTGG 
TTACAGTACA ATGAGGTCAA TGAGAGCAGC AGCGATGAAG GGAACGAGCC TGCCAATGAG 
TTTGCACAAC CAGAAGCTTT CATGTTGGAT GGGAACAACA ACCTGGAGGA CGACTCGAGC 
GTGAGTGAAG ACCTGGATGT GGACTGGAGC CTATTTGATG GTTTTGCGGA TGGACTTGGT 
GTTGCTGAAG CTATTTCCTA CGTGGATCCT CAGTTCCTCA CCTACATGGC ACTAGAAGAA 
CGCTTAGCCC AGGCTATGGA GACTGCACTG GCACACTTAG AGTCTCTTGC TGTAGACGTT 
GAGGTGGCTA ACCCACCTGC CAGTAAGGAA AGCATCGACG GACTTCCAGA AACCCTTGTC 
CTAGAAGATC ACACCGCTAT TGGTCAGGAG CAGTGCTGTC CCATCTGCTG CAGTGAGTAC 
ATTAAGGATG ACATCGCAAC AGAGCTGCCC TGCCATCACT TCTTTCATAA GCCTTGTGTC 
TCCATTTGGC TACAGAAGTC CGGAACGTGC CCTGTGTGCC GCCGCCACTT CCCACCTGCA 
GTGATTGACG CATCTGCAGC TGCTTCCTCT GAACCAGACC TTGATGCCTC GCCTGCAAAC 
GACAATGCTG AGGAAGCCCC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pja2 ( NM_001277278.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001264207.1 Rattus norvegicus praja ring finger ubiquitin ligase 2 (Pja2), transcript variant 2, mRNA.

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CloneID	ORa11812
Clone ID Related Accession (Same CDS sequence)	NM_001277278.1
Accession Version	NM_001277278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase Praja-2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB577390.1, BC074015.1 and AABR07068612.1. Transcript Variant: This variant (2) lacks an exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCACAGT ATACAGAAAA AGAACCATCA GTAATGGATC AAGACTCCAG CAAGGCTGCG 
TGGCCTAGAG CAGCAGGAGG ATACCAGACG ATTACAGGCA GGAGGTACGG AAGGAGACAT 
GCGTATGTCA GTTTTAAGCC ATGTATGACC AGGCATGAAC GGAGCTTGGG TCGGGCTGGC 
GATGACTATG AAGTTCTGGA ACTGGATGAC GTTGCCAAGG AAAACACCGC AGGTTCCAGT 
TCATTGGATC AAGTCCATCC TTCTTTACCC AGTGAAACTA CAGTTGAAAA AAGTGAAACA 
GAAATTCCTA CTTGTGGTCC AGCACTGAAT CAAAGCACGG AGAGCAACCC ATCCGTTGCC 
ACAGTGTGTC ATAGTGAGGA AGTCAGGGAG ACCTTAGACA GCAGTACGAA TCTTCAGAAT 
CACGCTGAGA GAGAGTGTAC GCCAGCAGTT TGTAATGCCT CAAGTGTCCA GAATGGAATT 
GTGTTGGTTC ATACTGACTC TTATGATCCA GACAGCAAGC ATGATGAGAA TGACTCTCTT 
CAACTTTGTG CCCAAGCTGT GGAAGGTGGT AGACGTCAGA AGGTATTAGG CAATGCAGTC 
TTTGAGCTGG AAAATGGAGA GGTAGAGAGA TATGCTGATC TGTGTCCCTC AGTTCCCTCT 
CTCAGTGGTG AAATAAGGGA GGAGTCTGAA GAGCTAGGTT CAGCACTCTT AGAGAAAAAT 
TCTGCTGGCG ATGCGGAGGC TGTCCATCAG GATGGGCAGG AATTTCAGAG GTCTTCTGAA 
GACGGCATTG TTAGAAAGAG GCGACAAGAT GATACCGATC AGGGAAGACA GACAGAAAAT 
TCAACTGAAG ATGCAGACTG TGTTCCAGGC CATGTTGAAC AAAATACTAG TGAGAGAGCC 
AATCACCATG GAAGTTCTCC TGAACAGGTA GTGAGGCCCA AAGTTAGAAA AGTGATCAGT 
TCAAGCCAGG TGGACCAAGA GAGCGGTTTT AATAGGCACG AGGCTAAGCA AAGAAGTGTT 
CAGAGGTGGA GAGAGGCTCT GGAAGTTGAG GAATGCAGTT CAGATGACCC TATAATCAAG 
TGTGACGATT ATGATGGAGA CCATGACTGC ATGTTCCTAA CCCCATCCTA CTCAAGAGTT 
ACGCCAAGGG AAGCAGAACG TCACCGTGCG ACAGCAGAAA ATGGAGCCAC AGCTTCAGGA 
AGGCAAGAGG CTCGGGAAAA TGCCTTTTGG AATGCCTGTG GAGAGTATTA CCAGCTCTTT 
GACAAAGACG AAGACAGAGA ACAGACGTCC TTGGAGGAGG GGGAGATTCC CTGGTTACAG 
TACAATGAGG TCAATGAGAG CAGCAGCGAT GAAGGGAACG AGCCTGCCAA TGAGTTTGCA 
CAACCAGAAG CTTTCATGTT GGATGGGAAC AACAACCTGG AGGACGACTC GAGCGTGAGT 
GAAGACCTGG ATGTGGACTG GAGCCTATTT GATGGTTTTG CGGATGGACT TGGTGTTGCT 
GAAGCTATTT CCTACGTGGA TCCTCAGTTC CTCACCTACA TGGCACTAGA AGAACGCTTA 
GCCCAGGCTA TGGAGACTGC ACTGGCACAC TTAGAGTCTC TTGCTGTAGA CGTTGAGGTG 
GCTAACCCAC CTGCCAGTAA GGAAAGCATC GACGGACTTC CAGAAACCCT TGTCCTAGAA 
GATCACACCG CTATTGGTCA GGAGCAGTGC TGTCCCATCT GCTGCAGTGA GTACATTAAG 
GATGACATCG CAACAGAGCT GCCCTGCCAT CACTTCTTTC ATAAGCCTTG TGTCTCCATT 
TGGCTACAGA AGTCCGGAAC GTGCCCTGTG TGCCGCCGCC ACTTCCCACC TGCAGTGATT 
GACGCATCTG CAGCTGCTTC CTCTGAACCA GACCTTGATG CCTCGCCTGC AAACGACAAT 
GCTGAGGAAG CCCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264207.1
CDS	205..2142
Translation	MSQYTEKEPSVMDQDSSKAAWPRAAGGYQTITGRRYGRRHAYVSFKPCMTRHERSLGRAGDDYEVLELDDVAKENTAGSSSLDQVHPSLPSETTVEKSETEIPTCGPALNQSTESNPSVATVCHSEEVRETLDSSTNLQNHAERECTPAVCNASSVQNGIVLVHTDSYDPDSKHDENDSLQLCAQAVEGGRRQKVLGNAVFELENGEVERYADLCPSVPSLSGEIREESEELGSALLEKNSAGDAEAVHQDGQEFQRSSEDGIVRKRRQDDTDQGRQTENSTEDADCVPGHVEQNTSERANHHGSSPEQVVRPKVRKVISSSQVDQESGFNRHEAKQRSVQRWREALEVEECSSDDPIIKCDDYDGDHDCMFLTPSYSRVTPREAERHRATAENGATASGRQEARENAFWNACGEYYQLFDKDEDREQTSLEEGEIPWLQYNEVNESSSDEGNEPANEFAQPEAFMLDGNNNLEDDSSVSEDLDVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLEDHTAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVIDASAAASSEPDLDASPANDNAEEAP

Target ORF information:

RefSeq Version	NM_001277278.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus praja ring finger ubiquitin ligase 2 (Pja2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277278.1

ORF Insert Sequence:

ATGTCACAGT ATACAGAAAA AGAACCATCA GTAATGGATC AAGACTCCAG CAAGGCTGCG 
TGGCCTAGAG CAGCAGGAGG ATACCAGACG ATTACAGGCA GGAGGTACGG AAGGAGACAT 
GCGTATGTCA GTTTTAAGCC ATGTATGACC AGGCATGAAC GGAGCTTGGG TCGGGCTGGC 
GATGACTATG AAGTTCTGGA ACTGGATGAC GTTGCCAAGG AAAACACCGC AGGTTCCAGT 
TCATTGGATC AAGTCCATCC TTCTTTACCC AGTGAAACTA CAGTTGAAAA AAGTGAAACA 
GAAATTCCTA CTTGTGGTCC AGCACTGAAT CAAAGCACGG AGAGCAACCC ATCCGTTGCC 
ACAGTGTGTC ATAGTGAGGA AGTCAGGGAG ACCTTAGACA GCAGTACGAA TCTTCAGAAT 
CACGCTGAGA GAGAGTGTAC GCCAGCAGTT TGTAATGCCT CAAGTGTCCA GAATGGAATT 
GTGTTGGTTC ATACTGACTC TTATGATCCA GACAGCAAGC ATGATGAGAA TGACTCTCTT 
CAACTTTGTG CCCAAGCTGT GGAAGGTGGT AGACGTCAGA AGGTATTAGG CAATGCAGTC 
TTTGAGCTGG AAAATGGAGA GGTAGAGAGA TATGCTGATC TGTGTCCCTC AGTTCCCTCT 
CTCAGTGGTG AAATAAGGGA GGAGTCTGAA GAGCTAGGTT CAGCACTCTT AGAGAAAAAT 
TCTGCTGGCG ATGCGGAGGC TGTCCATCAG GATGGGCAGG AATTTCAGAG GTCTTCTGAA 
GACGGCATTG TTAGAAAGAG GCGACAAGAT GATACCGATC AGGGAAGACA GACAGAAAAT 
TCAACTGAAG ATGCAGACTG TGTTCCAGGC CATGTTGAAC AAAATACTAG TGAGAGAGCC 
AATCACCATG GAAGTTCTCC TGAACAGGTA GTGAGGCCCA AAGTTAGAAA AGTGATCAGT 
TCAAGCCAGG TGGACCAAGA GAGCGGTTTT AATAGGCACG AGGCTAAGCA AAGAAGTGTT 
CAGAGGTGGA GAGAGGCTCT GGAAGTTGAG GAATGCAGTT CAGATGACCC TATAATCAAG 
TGTGACGATT ATGATGGAGA CCATGACTGC ATGTTCCTAA CCCCATCCTA CTCAAGAGTT 
ACGCCAAGGG AAGCAGAACG TCACCGTGCG ACAGCAGAAA ATGGAGCCAC AGCTTCAGGA 
AGGCAAGAGG CTCGGGAAAA TGCCTTTTGG AATGCCTGTG GAGAGTATTA CCAGCTCTTT 
GACAAAGACG AAGACAGAGA ACAGACGTCC TTGGAGGAGG GGGAGATTCC CTGGTTACAG 
TACAATGAGG TCAATGAGAG CAGCAGCGAT GAAGGGAACG AGCCTGCCAA TGAGTTTGCA 
CAACCAGAAG CTTTCATGTT GGATGGGAAC AACAACCTGG AGGACGACTC GAGCGTGAGT 
GAAGACCTGG ATGTGGACTG GAGCCTATTT GATGGTTTTG CGGATGGACT TGGTGTTGCT 
GAAGCTATTT CCTACGTGGA TCCTCAGTTC CTCACCTACA TGGCACTAGA AGAACGCTTA 
GCCCAGGCTA TGGAGACTGC ACTGGCACAC TTAGAGTCTC TTGCTGTAGA CGTTGAGGTG 
GCTAACCCAC CTGCCAGTAA GGAAAGCATC GACGGACTTC CAGAAACCCT TGTCCTAGAA 
GATCACACCG CTATTGGTCA GGAGCAGTGC TGTCCCATCT GCTGCAGTGA GTACATTAAG 
GATGACATCG CAACAGAGCT GCCCTGCCAT CACTTCTTTC ATAAGCCTTG TGTCTCCATT 
TGGCTACAGA AGTCCGGAAC GTGCCCTGTG TGCCGCCGCC ACTTCCCACC TGCAGTGATT 
GACGCATCTG CAGCTGCTTC CTCTGAACCA GACCTTGATG CCTCGCCTGC AAACGACAAT 
GCTGAGGAAG CCCCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ubiquitylation of MFHAS1 by the ubiquitin ligase praja2 promotes M1 macrophage polarization by activating JNK and p38 pathways.
Cell death & disease8(5)e2763(2017 May)
Zhong J,Wang H,Chen W,Sun Z,Chen J,Xu Y,Weng M,Shi Q,Ma D,Miao C

Control of PKA stability and signalling by the RING ligase praja2.
Nature cell biology13(4)412-22(2011 Apr)
Lignitto L,Carlucci A,Sepe M,Stefan E,Cuomo O,Nistic? R,Scorziello A,Savoia C,Garbi C,Annunziato L,Feliciello A

A novel RING-H2 motif protein downregulated by axotomy: its characteristic localization at the postsynaptic density of axosomatic synapse.
The Journal of neuroscience : the official journal of the Society for Neuroscience15(7 Pt 2)5238-48(1995 Jul)
Nakayama M,Miyake T,Gahara Y,Ohara O,Kitamura T

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Pja2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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