PJA2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PJA2
Entrez Gene ID	9867
Full Name	praja ring finger ubiquitin ligase 2
Synonyms	Neurodap1,RNF131
General protein information	Preferred Names praja ring finger ubiquitin ligase 2 Names E3 ubiquitin-protein ligase Praja-2 RING-type E3 ubiquitin transferase Praja-2 praja 2, RING-H2 motif containing praja ring finger 2, E3 ubiquitin protein ligase praja2 ring finger protein 131
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q21.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_014819.5	NP_055634.3	E3 ubiquitin-protein ligase Praja-2
NM_014819.4	NP_055634.3	E3 ubiquitin-protein ligase Praja-2
XM_017010099.1	XP_016865588.1	E3 ubiquitin-protein ligase Praja-2 isoform X1
XM_017010099.2	XP_016865588.1	E3 ubiquitin-protein ligase Praja-2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PJA2	NP_055634.3
Pan troglodytes (chimpanzee)	PJA2	XP_526975.2
	PJA2	XP_001100134.1
Canis lupus familiaris (dog)	PJA2	XP_536288.3
Bos taurus (cattle)	PJA2	NP_001093810.1
Rattus norvegicus (Norway rat)	Pja2	NP_620251.1
Xenopus tropicalis (tropical clawed frog)	pja2	XP_004910519.1
Gallus gallus (chicken)	PJA2	XP_413980.3
Danio rerio (zebrafish)	pja2	XP_005165620.1
Danio rerio (zebrafish)	LOC101882388	XP_005165623.1
Mus musculus (house mouse)	Pja2	NP_001020480.1

Gene Ontology
Bibliography

Related articles in PubMed

Ubiquitylation of MFHAS1 by the ubiquitin ligase praja2 promotes M1 macrophage polarization by activating JNK and p38 pathways.
Zhong J, Wang H, Chen W, Sun Z, Chen J, Xu Y, Weng M, Shi Q, Ma D, Miao C
Cell death & disease8(5)e2763(2017 May)

PJA2 ubiquitinates the HIV-1 Tat protein with atypical chain linkages to activate viral transcription.
Faust TB, Li Y, Jang GM, Johnson JR, Yang S, Weiss A, Krogan NJ, Frankel AD
Scientific reports745394(2017 Mar)

praja2 regulates KSR1 stability and mitogenic signaling.
Rinaldi L, Delle Donne R, Sepe M, Porpora M, Garbi C, Chiuso F, Gallo A, Parisi S, Russo L, Bachmann V, Huber RG, Stefan E, Russo T, Feliciello A
Cell death & disease7e2230(2016 May)

Detection of novel paraja ring finger 2-fer tyrosine kinase mRNA chimeras is associated with poor postoperative prognosis in non-small cell lung cancer.
Kawakami M, Ishikawa R, Amano Y, Sunohara M, Watanabe K, Ohishi N, Yatomi Y, Nakajima J, Fukayama M, Nagase T, Takai D
Cancer science104(11)1447-54(2013 Nov)

Proteolysis of MOB1 by the ubiquitin ligase praja2 attenuates Hippo signalling and supports glioblastoma growth.
Lignitto L, Arcella A, Sepe M, Rinaldi L, Delle Donne R, Gallo A, Stefan E, Bachmann VA, Oliva MA, Tiziana Storlazzi C, L'Abbate A, Brunetti A, Gargiulo S, Gramanzini M, Insabato L, Garbi C, Gottesman ME, Feliciello A
Nature communications41822(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

praja2 regulates KSR1 stability and mitogenic signaling.
Title: praja2 regulates KSR1 stability and mitogenic signaling.

Detection of PJA2-FER fusion mRNA is correlated with poor postoperative survival periods in non-small cell lung cancer.
Title: Detection of novel paraja ring finger 2-fer tyrosine kinase mRNA chimeras is associated with poor postoperative prognosis in non-small cell lung cancer.

The RING ligase praja2 ubiquitylates and degrades Mob, a core component of NDR/LATS kinase and a positive regulator of the tumour-suppressor Hippo cascade.
Title: Proteolysis of MOB1 by the ubiquitin ligase praja2 attenuates Hippo signalling and supports glioblastoma growth.

praja2 is a novel cancer-related gene whose expression is linked to the histotype and mutational status of the thyroid tumor.
Title: Expression of the ring ligase PRAJA2 in thyroid cancer.

The following PJA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PJA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17380	NM_014819.5 Latest version!	Homo sapiens praja ring finger ubiquitin ligase 2 (PJA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu17380	XM_017010099.2 Latest version!	Homo sapiens praja ring finger ubiquitin ligase 2 (PJA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
View Old Accession Versions >>
OHu17380	NM_014819.4	Homo sapiens praja ring finger ubiquitin ligase 2 (PJA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu17380	XM_017010099.1	Homo sapiens praja ring finger ubiquitin ligase 2 (PJA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PJA2 ( NM_014819.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055634.3 Homo sapiens praja ring finger ubiquitin ligase 2 (PJA2), mRNA.

Price & Availability↑

CloneID	OHu17380
Clone ID Related Accession (Same CDS sequence)	NM_014819.5 , NM_014819.4 , XM_017010099.1 , XM_017010099.2
Accession Version	NM_014819.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Praja-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM469517.1, AB007898.1, BQ228553.1 and BP301854.1. On Nov 22, 2018 this sequence version replaced NM_014819.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.192241.1, SRR1660803.125461.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361189.7/ ENSP00000354775.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCACAGT ACACTGAAAA GGAGCCAGCA GCAATGGACC AAGAATCTGG TAAGGCTGTC 
TGGCCCAAAC CAGCAGGAGG GTATCAGACA ATTACAGGCA GGAGATATGG AAGAAGACAT 
GCTTATGTCA GTTTTAAACC ATGTATGACC AGACATGAAA GAAGCTTAGG TCGGGCTGGT 
GATGACTATG AAGTGTTGGA ACTAGATGAT GTTCCAAAGG AAAATTCCTC AGGTTCCAGT 
CCTTTGGATC AAGTTGATTC TTCTTTACCC AGTGAACCTA TATTTGAAAA AAGTGAAACA 
GAAATTCCCA CTTGTGGTTC AGCATTGAAT CAAACCACTG AGAGCAGTCA ATCCTTTGTT 
GCAGTACATC ACAGTGAGGA AGGCAGGGAT ACCTTAGGAA GCAGTACAAA TCTTCATAAT 
CACTCTGAGG GAGAGTATAT TCCAGGAGCT TGTAGTGCTT CAAGTGTCCA AAATGGAATT 
GCATTGGTTC ATACAGACTC TTATGATCCA GATGGCAAAC ATGGAGAAGA TAATGACCAT 
CTTCAACTTT CTGCAGAAGT CGTGGAAGGT AGTAGATACC AGGAATCATT AGGCAATACA 
GTATTTGAGT TGGAAAACAG AGAGGCAGAG GCATACACTG GTCTTTCACC ACCAGTTCCC 
TCATTTAACT GTGAAGTAAG AGATGAGTTT GAAGAGTTAG ATTCTGTACC ATTAGTGAAA 
AGTTCTGCTG GTGATACTGA GTTTGTCCAT CAGAATAGCC AGGAAATTCA GAGGTCTTCT 
CAAGATGAAA TGGTTAGTAC GAAACAACAA AATAATACTA GCCAGGAAAG ACAGACAGAA 
CATTCACCTG AAGATGCAGC CTGTGGTCCA GGGCATATTT GTAGTGAACA AAATACCAAT 
GATAGGGAAA AGAACCATGG AAGTTCTCCT GAACAGGTAG TGAGGCCAAA AGTTAGAAAA 
CTGATAAGTT CAAGCCAGGT GGACCAAGAA ACAGGTTTTA ATAGGCATGA GGCGAAACAA 
AGAAGTGTTC AAAGATGGAG AGAGGCTTTG GAAGTTGAGG AAAGTGGCTC AGATGACCTC 
TTAATAAAAT GTGAAGAATA TGATGGAGAG CATGACTGTA TGTTCTTGGA TCCACCATAC 
TCAAGAGTTA TTACACAAAG GGAAACAGAA AATAACCAAA TGACATCAGA AAGTGGAGCC 
ACAGCAGGAA GGCAAGAGGT GGATAACACC TTTTGGAATG GCTGTGGAGA TTATTACCAA 
CTCTATGACA AAGATGAAGA TAGTTCTGAA TGCAGTGATG GGGAATGGTC TGCTTCTTTG 
CCTCATCGAT TTTCTGGTAC AGAAAAAGAT CAATCCTCAA GTGATGAAAG CTGGGAGACT 
CTGCCAGGAA AAGATGAGAA TGAACCTGAG CTACAAAGTG ATAGCAGTGG CCCTGAAGAA 
GAAAACCAAG AATTATCTCT TCAGGAAGGG GAACAGACAT CCTTGGAAGA GGGAGAAATT 
CCTTGGTTAC AGTACAATGA AGTCAATGAA AGCAGCAGTG ATGAGGGAAA TGAACCTGCC 
AATGAATTTG CACAGCCAGC TTTCATGTTG GATGGTAACA ATAACCTGGA GGATGACTCC 
AGTGTGAGTG AAGACTTAGA TGTGGATTGG AGCCTATTTG ATGGCTTTGC AGATGGACTA 
GGAGTTGCTG AAGCTATTTC ATATGTGGAT CCTCAGTTCC TTACCTACAT GGCACTAGAA 
GAACGCTTAG CCCAGGCTAT GGAGACTGCT CTGGCCCATT TAGAGTCTCT TGCAGTGGAT 
GTTGAGGTGG CCAATCCACC AGCTAGTAAG GAAAGCATTG ATGGTCTTCC AGAGACCCTT 
GTTCTTGAAG ATCACACTGC TATTGGTCAG GAACAATGCT GTCCAATCTG TTGCAGTGAG 
TATATTAAGG ATGATATAGC AACAGAGTTG CCCTGTCACC ATTTCTTTCA CAAACCTTGT 
GTCTCAATTT GGCTACAAAA GTCGGGAACA TGCCCTGTGT GCCGCCGTCA TTTCCCACCT 
GCGGTTATTG AAGCATCTGC AGCTCCTTCC TCTGAGCCTG ATCCTGATGC CCCACCTTCA 
AATGACAGTA TTGCAGAAGC ACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055634.3
CDS	221..2347
Translation	MSQYTEKEPAAMDQESGKAVWPKPAGGYQTITGRRYGRRHAYVSFKPCMTRHERSLGRAGDDYEVLELDDVPKENSSGSSPLDQVDSSLPSEPIFEKSETEIPTCGSALNQTTESSQSFVAVHHSEEGRDTLGSSTNLHNHSEGEYIPGACSASSVQNGIALVHTDSYDPDGKHGEDNDHLQLSAEVVEGSRYQESLGNTVFELENREAEAYTGLSPPVPSFNCEVRDEFEELDSVPLVKSSAGDTEFVHQNSQEIQRSSQDEMVSTKQQNNTSQERQTEHSPEDAACGPGHICSEQNTNDREKNHGSSPEQVVRPKVRKLISSSQVDQETGFNRHEAKQRSVQRWREALEVEESGSDDLLIKCEEYDGEHDCMFLDPPYSRVITQRETENNQMTSESGATAGRQEVDNTFWNGCGDYYQLYDKDEDSSECSDGEWSASLPHRFSGTEKDQSSSDESWETLPGKDENEPELQSDSSGPEEENQELSLQEGEQTSLEEGEIPWLQYNEVNESSSDEGNEPANEFAQPAFMLDGNNNLEDDSSVSEDLDVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLEDHTAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVIEASAAPSSEPDPDAPPSNDSIAEAP

Target ORF information:

RefSeq Version	NM_014819.5
Organism	Homo sapiens(human)
Definition	Homo sapiens praja ring finger ubiquitin ligase 2 (PJA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014819.5

ORF Insert Sequence:

ATGTCACAGT ACACTGAAAA GGAGCCAGCA GCAATGGACC AAGAATCTGG TAAGGCTGTC 
TGGCCCAAAC CAGCAGGAGG GTATCAGACA ATTACAGGCA GGAGATATGG AAGAAGACAT 
GCTTATGTCA GTTTTAAACC ATGTATGACC AGACATGAAA GAAGCTTAGG TCGGGCTGGT 
GATGACTATG AAGTGTTGGA ACTAGATGAT GTTCCAAAGG AAAATTCCTC AGGTTCCAGT 
CCTTTGGATC AAGTTGATTC TTCTTTACCC AGTGAACCTA TATTTGAAAA AAGTGAAACA 
GAAATTCCCA CTTGTGGTTC AGCATTGAAT CAAACCACTG AGAGCAGTCA ATCCTTTGTT 
GCAGTACATC ACAGTGAGGA AGGCAGGGAT ACCTTAGGAA GCAGTACAAA TCTTCATAAT 
CACTCTGAGG GAGAGTATAT TCCAGGAGCT TGTAGTGCTT CAAGTGTCCA AAATGGAATT 
GCATTGGTTC ATACAGACTC TTATGATCCA GATGGCAAAC ATGGAGAAGA TAATGACCAT 
CTTCAACTTT CTGCAGAAGT CGTGGAAGGT AGTAGATACC AGGAATCATT AGGCAATACA 
GTATTTGAGT TGGAAAACAG AGAGGCAGAG GCATACACTG GTCTTTCACC ACCAGTTCCC 
TCATTTAACT GTGAAGTAAG AGATGAGTTT GAAGAGTTAG ATTCTGTACC ATTAGTGAAA 
AGTTCTGCTG GTGATACTGA GTTTGTCCAT CAGAATAGCC AGGAAATTCA GAGGTCTTCT 
CAAGATGAAA TGGTTAGTAC GAAACAACAA AATAATACTA GCCAGGAAAG ACAGACAGAA 
CATTCACCTG AAGATGCAGC CTGTGGTCCA GGGCATATTT GTAGTGAACA AAATACCAAT 
GATAGGGAAA AGAACCATGG AAGTTCTCCT GAACAGGTAG TGAGGCCAAA AGTTAGAAAA 
CTGATAAGTT CAAGCCAGGT GGACCAAGAA ACAGGTTTTA ATAGGCATGA GGCGAAACAA 
AGAAGTGTTC AAAGATGGAG AGAGGCTTTG GAAGTTGAGG AAAGTGGCTC AGATGACCTC 
TTAATAAAAT GTGAAGAATA TGATGGAGAG CATGACTGTA TGTTCTTGGA TCCACCATAC 
TCAAGAGTTA TTACACAAAG GGAAACAGAA AATAACCAAA TGACATCAGA AAGTGGAGCC 
ACAGCAGGAA GGCAAGAGGT GGATAACACC TTTTGGAATG GCTGTGGAGA TTATTACCAA 
CTCTATGACA AAGATGAAGA TAGTTCTGAA TGCAGTGATG GGGAATGGTC TGCTTCTTTG 
CCTCATCGAT TTTCTGGTAC AGAAAAAGAT CAATCCTCAA GTGATGAAAG CTGGGAGACT 
CTGCCAGGAA AAGATGAGAA TGAACCTGAG CTACAAAGTG ATAGCAGTGG CCCTGAAGAA 
GAAAACCAAG AATTATCTCT TCAGGAAGGG GAACAGACAT CCTTGGAAGA GGGAGAAATT 
CCTTGGTTAC AGTACAATGA AGTCAATGAA AGCAGCAGTG ATGAGGGAAA TGAACCTGCC 
AATGAATTTG CACAGCCAGC TTTCATGTTG GATGGTAACA ATAACCTGGA GGATGACTCC 
AGTGTGAGTG AAGACTTAGA TGTGGATTGG AGCCTATTTG ATGGCTTTGC AGATGGACTA 
GGAGTTGCTG AAGCTATTTC ATATGTGGAT CCTCAGTTCC TTACCTACAT GGCACTAGAA 
GAACGCTTAG CCCAGGCTAT GGAGACTGCT CTGGCCCATT TAGAGTCTCT TGCAGTGGAT 
GTTGAGGTGG CCAATCCACC AGCTAGTAAG GAAAGCATTG ATGGTCTTCC AGAGACCCTT 
GTTCTTGAAG ATCACACTGC TATTGGTCAG GAACAATGCT GTCCAATCTG TTGCAGTGAG 
TATATTAAGG ATGATATAGC AACAGAGTTG CCCTGTCACC ATTTCTTTCA CAAACCTTGT 
GTCTCAATTT GGCTACAAAA GTCGGGAACA TGCCCTGTGT GCCGCCGTCA TTTCCCACCT 
GCGGTTATTG AAGCATCTGC AGCTCCTTCC TCTGAGCCTG ATCCTGATGC CCCACCTTCA 
AATGACAGTA TTGCAGAAGC ACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PJA2 ( NM_014819.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055634.3 Homo sapiens praja ring finger ubiquitin ligase 2 (PJA2), mRNA.

Price & Availability↑

CloneID	OHu17380
Clone ID Related Accession (Same CDS sequence)	NM_014819.5 , NM_014819.4 , XM_017010099.1 , XM_017010099.2
Accession Version	NM_014819.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Praja-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM469517.1, AB007898.1, BQ228553.1, BP301854.1 and BM681899.1. On Sep 21, 2007 this sequence version replaced NM_014819.3. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.156052.1, SRR1660807.192241.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGTCACAGT ACACTGAAAA GGAGCCAGCA GCAATGGACC AAGAATCTGG TAAGGCTGTC 
TGGCCCAAAC CAGCAGGAGG GTATCAGACA ATTACAGGCA GGAGATATGG AAGAAGACAT 
GCTTATGTCA GTTTTAAACC ATGTATGACC AGACATGAAA GAAGCTTAGG TCGGGCTGGT 
GATGACTATG AAGTGTTGGA ACTAGATGAT GTTCCAAAGG AAAATTCCTC AGGTTCCAGT 
CCTTTGGATC AAGTTGATTC TTCTTTACCC AGTGAACCTA TATTTGAAAA AAGTGAAACA 
GAAATTCCCA CTTGTGGTTC AGCATTGAAT CAAACCACTG AGAGCAGTCA ATCCTTTGTT 
GCAGTACATC ACAGTGAGGA AGGCAGGGAT ACCTTAGGAA GCAGTACAAA TCTTCATAAT 
CACTCTGAGG GAGAGTATAT TCCAGGAGCT TGTAGTGCTT CAAGTGTCCA AAATGGAATT 
GCATTGGTTC ATACAGACTC TTATGATCCA GATGGCAAAC ATGGAGAAGA TAATGACCAT 
CTTCAACTTT CTGCAGAAGT CGTGGAAGGT AGTAGATACC AGGAATCATT AGGCAATACA 
GTATTTGAGT TGGAAAACAG AGAGGCAGAG GCATACACTG GTCTTTCACC ACCAGTTCCC 
TCATTTAACT GTGAAGTAAG AGATGAGTTT GAAGAGTTAG ATTCTGTACC ATTAGTGAAA 
AGTTCTGCTG GTGATACTGA GTTTGTCCAT CAGAATAGCC AGGAAATTCA GAGGTCTTCT 
CAAGATGAAA TGGTTAGTAC GAAACAACAA AATAATACTA GCCAGGAAAG ACAGACAGAA 
CATTCACCTG AAGATGCAGC CTGTGGTCCA GGGCATATTT GTAGTGAACA AAATACCAAT 
GATAGGGAAA AGAACCATGG AAGTTCTCCT GAACAGGTAG TGAGGCCAAA AGTTAGAAAA 
CTGATAAGTT CAAGCCAGGT GGACCAAGAA ACAGGTTTTA ATAGGCATGA GGCGAAACAA 
AGAAGTGTTC AAAGATGGAG AGAGGCTTTG GAAGTTGAGG AAAGTGGCTC AGATGACCTC 
TTAATAAAAT GTGAAGAATA TGATGGAGAG CATGACTGTA TGTTCTTGGA TCCACCATAC 
TCAAGAGTTA TTACACAAAG GGAAACAGAA AATAACCAAA TGACATCAGA AAGTGGAGCC 
ACAGCAGGAA GGCAAGAGGT GGATAACACC TTTTGGAATG GCTGTGGAGA TTATTACCAA 
CTCTATGACA AAGATGAAGA TAGTTCTGAA TGCAGTGATG GGGAATGGTC TGCTTCTTTG 
CCTCATCGAT TTTCTGGTAC AGAAAAAGAT CAATCCTCAA GTGATGAAAG CTGGGAGACT 
CTGCCAGGAA AAGATGAGAA TGAACCTGAG CTACAAAGTG ATAGCAGTGG CCCTGAAGAA 
GAAAACCAAG AATTATCTCT TCAGGAAGGG GAACAGACAT CCTTGGAAGA GGGAGAAATT 
CCTTGGTTAC AGTACAATGA AGTCAATGAA AGCAGCAGTG ATGAGGGAAA TGAACCTGCC 
AATGAATTTG CACAGCCAGC TTTCATGTTG GATGGTAACA ATAACCTGGA GGATGACTCC 
AGTGTGAGTG AAGACTTAGA TGTGGATTGG AGCCTATTTG ATGGCTTTGC AGATGGACTA 
GGAGTTGCTG AAGCTATTTC ATATGTGGAT CCTCAGTTCC TTACCTACAT GGCACTAGAA 
GAACGCTTAG CCCAGGCTAT GGAGACTGCT CTGGCCCATT TAGAGTCTCT TGCAGTGGAT 
GTTGAGGTGG CCAATCCACC AGCTAGTAAG GAAAGCATTG ATGGTCTTCC AGAGACCCTT 
GTTCTTGAAG ATCACACTGC TATTGGTCAG GAACAATGCT GTCCAATCTG TTGCAGTGAG 
TATATTAAGG ATGATATAGC AACAGAGTTG CCCTGTCACC ATTTCTTTCA CAAACCTTGT 
GTCTCAATTT GGCTACAAAA GTCGGGAACA TGCCCTGTGT GCCGCCGTCA TTTCCCACCT 
GCGGTTATTG AAGCATCTGC AGCTCCTTCC TCTGAGCCTG ATCCTGATGC CCCACCTTCA 
AATGACAGTA TTGCAGAAGC ACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055634.3
CDS	221..2347
Translation	MSQYTEKEPAAMDQESGKAVWPKPAGGYQTITGRRYGRRHAYVSFKPCMTRHERSLGRAGDDYEVLELDDVPKENSSGSSPLDQVDSSLPSEPIFEKSETEIPTCGSALNQTTESSQSFVAVHHSEEGRDTLGSSTNLHNHSEGEYIPGACSASSVQNGIALVHTDSYDPDGKHGEDNDHLQLSAEVVEGSRYQESLGNTVFELENREAEAYTGLSPPVPSFNCEVRDEFEELDSVPLVKSSAGDTEFVHQNSQEIQRSSQDEMVSTKQQNNTSQERQTEHSPEDAACGPGHICSEQNTNDREKNHGSSPEQVVRPKVRKLISSSQVDQETGFNRHEAKQRSVQRWREALEVEESGSDDLLIKCEEYDGEHDCMFLDPPYSRVITQRETENNQMTSESGATAGRQEVDNTFWNGCGDYYQLYDKDEDSSECSDGEWSASLPHRFSGTEKDQSSSDESWETLPGKDENEPELQSDSSGPEEENQELSLQEGEQTSLEEGEIPWLQYNEVNESSSDEGNEPANEFAQPAFMLDGNNNLEDDSSVSEDLDVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLEDHTAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVIEASAAPSSEPDPDAPPSNDSIAEAP

Target ORF information:

RefSeq Version	NM_014819.4
Organism	Homo sapiens(human)
Definition	Homo sapiens praja ring finger ubiquitin ligase 2 (PJA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014819.4

ORF Insert Sequence:

ATGTCACAGT ACACTGAAAA GGAGCCAGCA GCAATGGACC AAGAATCTGG TAAGGCTGTC 
TGGCCCAAAC CAGCAGGAGG GTATCAGACA ATTACAGGCA GGAGATATGG AAGAAGACAT 
GCTTATGTCA GTTTTAAACC ATGTATGACC AGACATGAAA GAAGCTTAGG TCGGGCTGGT 
GATGACTATG AAGTGTTGGA ACTAGATGAT GTTCCAAAGG AAAATTCCTC AGGTTCCAGT 
CCTTTGGATC AAGTTGATTC TTCTTTACCC AGTGAACCTA TATTTGAAAA AAGTGAAACA 
GAAATTCCCA CTTGTGGTTC AGCATTGAAT CAAACCACTG AGAGCAGTCA ATCCTTTGTT 
GCAGTACATC ACAGTGAGGA AGGCAGGGAT ACCTTAGGAA GCAGTACAAA TCTTCATAAT 
CACTCTGAGG GAGAGTATAT TCCAGGAGCT TGTAGTGCTT CAAGTGTCCA AAATGGAATT 
GCATTGGTTC ATACAGACTC TTATGATCCA GATGGCAAAC ATGGAGAAGA TAATGACCAT 
CTTCAACTTT CTGCAGAAGT CGTGGAAGGT AGTAGATACC AGGAATCATT AGGCAATACA 
GTATTTGAGT TGGAAAACAG AGAGGCAGAG GCATACACTG GTCTTTCACC ACCAGTTCCC 
TCATTTAACT GTGAAGTAAG AGATGAGTTT GAAGAGTTAG ATTCTGTACC ATTAGTGAAA 
AGTTCTGCTG GTGATACTGA GTTTGTCCAT CAGAATAGCC AGGAAATTCA GAGGTCTTCT 
CAAGATGAAA TGGTTAGTAC GAAACAACAA AATAATACTA GCCAGGAAAG ACAGACAGAA 
CATTCACCTG AAGATGCAGC CTGTGGTCCA GGGCATATTT GTAGTGAACA AAATACCAAT 
GATAGGGAAA AGAACCATGG AAGTTCTCCT GAACAGGTAG TGAGGCCAAA AGTTAGAAAA 
CTGATAAGTT CAAGCCAGGT GGACCAAGAA ACAGGTTTTA ATAGGCATGA GGCGAAACAA 
AGAAGTGTTC AAAGATGGAG AGAGGCTTTG GAAGTTGAGG AAAGTGGCTC AGATGACCTC 
TTAATAAAAT GTGAAGAATA TGATGGAGAG CATGACTGTA TGTTCTTGGA TCCACCATAC 
TCAAGAGTTA TTACACAAAG GGAAACAGAA AATAACCAAA TGACATCAGA AAGTGGAGCC 
ACAGCAGGAA GGCAAGAGGT GGATAACACC TTTTGGAATG GCTGTGGAGA TTATTACCAA 
CTCTATGACA AAGATGAAGA TAGTTCTGAA TGCAGTGATG GGGAATGGTC TGCTTCTTTG 
CCTCATCGAT TTTCTGGTAC AGAAAAAGAT CAATCCTCAA GTGATGAAAG CTGGGAGACT 
CTGCCAGGAA AAGATGAGAA TGAACCTGAG CTACAAAGTG ATAGCAGTGG CCCTGAAGAA 
GAAAACCAAG AATTATCTCT TCAGGAAGGG GAACAGACAT CCTTGGAAGA GGGAGAAATT 
CCTTGGTTAC AGTACAATGA AGTCAATGAA AGCAGCAGTG ATGAGGGAAA TGAACCTGCC 
AATGAATTTG CACAGCCAGC TTTCATGTTG GATGGTAACA ATAACCTGGA GGATGACTCC 
AGTGTGAGTG AAGACTTAGA TGTGGATTGG AGCCTATTTG ATGGCTTTGC AGATGGACTA 
GGAGTTGCTG AAGCTATTTC ATATGTGGAT CCTCAGTTCC TTACCTACAT GGCACTAGAA 
GAACGCTTAG CCCAGGCTAT GGAGACTGCT CTGGCCCATT TAGAGTCTCT TGCAGTGGAT 
GTTGAGGTGG CCAATCCACC AGCTAGTAAG GAAAGCATTG ATGGTCTTCC AGAGACCCTT 
GTTCTTGAAG ATCACACTGC TATTGGTCAG GAACAATGCT GTCCAATCTG TTGCAGTGAG 
TATATTAAGG ATGATATAGC AACAGAGTTG CCCTGTCACC ATTTCTTTCA CAAACCTTGT 
GTCTCAATTT GGCTACAAAA GTCGGGAACA TGCCCTGTGT GCCGCCGTCA TTTCCCACCT 
GCGGTTATTG AAGCATCTGC AGCTCCTTCC TCTGAGCCTG ATCCTGATGC CCCACCTTCA 
AATGACAGTA TTGCAGAAGC ACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PJA2 ( XM_017010099.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865588.1 Homo sapiens praja ring finger ubiquitin ligase 2 (PJA2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu17380
Clone ID Related Accession (Same CDS sequence)	NM_014819.5 , NM_014819.4 , XM_017010099.1 , XM_017010099.2
Accession Version	XM_017010099.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Praja-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACAGT ACACTGAAAA GGAGCCAGCA GCAATGGACC AAGAATCTGG TAAGGCTGTC 
TGGCCCAAAC CAGCAGGAGG GTATCAGACA ATTACAGGCA GGAGATATGG AAGAAGACAT 
GCTTATGTCA GTTTTAAACC ATGTATGACC AGACATGAAA GAAGCTTAGG TCGGGCTGGT 
GATGACTATG AAGTGTTGGA ACTAGATGAT GTTCCAAAGG AAAATTCCTC AGGTTCCAGT 
CCTTTGGATC AAGTTGATTC TTCTTTACCC AGTGAACCTA TATTTGAAAA AAGTGAAACA 
GAAATTCCCA CTTGTGGTTC AGCATTGAAT CAAACCACTG AGAGCAGTCA ATCCTTTGTT 
GCAGTACATC ACAGTGAGGA AGGCAGGGAT ACCTTAGGAA GCAGTACAAA TCTTCATAAT 
CACTCTGAGG GAGAGTATAT TCCAGGAGCT TGTAGTGCTT CAAGTGTCCA AAATGGAATT 
GCATTGGTTC ATACAGACTC TTATGATCCA GATGGCAAAC ATGGAGAAGA TAATGACCAT 
CTTCAACTTT CTGCAGAAGT CGTGGAAGGT AGTAGATACC AGGAATCATT AGGCAATACA 
GTATTTGAGT TGGAAAACAG AGAGGCAGAG GCATACACTG GTCTTTCACC ACCAGTTCCC 
TCATTTAACT GTGAAGTAAG AGATGAGTTT GAAGAGTTAG ATTCTGTACC ATTAGTGAAA 
AGTTCTGCTG GTGATACTGA GTTTGTCCAT CAGAATAGCC AGGAAATTCA GAGGTCTTCT 
CAAGATGAAA TGGTTAGTAC GAAACAACAA AATAATACTA GCCAGGAAAG ACAGACAGAA 
CATTCACCTG AAGATGCAGC CTGTGGTCCA GGGCATATTT GTAGTGAACA AAATACCAAT 
GATAGGGAAA AGAACCATGG AAGTTCTCCT GAACAGGTAG TGAGGCCAAA AGTTAGAAAA 
CTGATAAGTT CAAGCCAGGT GGACCAAGAA ACAGGTTTTA ATAGGCATGA GGCGAAACAA 
AGAAGTGTTC AAAGATGGAG AGAGGCTTTG GAAGTTGAGG AAAGTGGCTC AGATGACCTC 
TTAATAAAAT GTGAAGAATA TGATGGAGAG CATGACTGTA TGTTCTTGGA TCCACCATAC 
TCAAGAGTTA TTACACAAAG GGAAACAGAA AATAACCAAA TGACATCAGA AAGTGGAGCC 
ACAGCAGGAA GGCAAGAGGT GGATAACACC TTTTGGAATG GCTGTGGAGA TTATTACCAA 
CTCTATGACA AAGATGAAGA TAGTTCTGAA TGCAGTGATG GGGAATGGTC TGCTTCTTTG 
CCTCATCGAT TTTCTGGTAC AGAAAAAGAT CAATCCTCAA GTGATGAAAG CTGGGAGACT 
CTGCCAGGAA AAGATGAGAA TGAACCTGAG CTACAAAGTG ATAGCAGTGG CCCTGAAGAA 
GAAAACCAAG AATTATCTCT TCAGGAAGGG GAACAGACAT CCTTGGAAGA GGGAGAAATT 
CCTTGGTTAC AGTACAATGA AGTCAATGAA AGCAGCAGTG ATGAGGGAAA TGAACCTGCC 
AATGAATTTG CACAGCCAGC TTTCATGTTG GATGGTAACA ATAACCTGGA GGATGACTCC 
AGTGTGAGTG AAGACTTAGA TGTGGATTGG AGCCTATTTG ATGGCTTTGC AGATGGACTA 
GGAGTTGCTG AAGCTATTTC ATATGTGGAT CCTCAGTTCC TTACCTACAT GGCACTAGAA 
GAACGCTTAG CCCAGGCTAT GGAGACTGCT CTGGCCCATT TAGAGTCTCT TGCAGTGGAT 
GTTGAGGTGG CCAATCCACC AGCTAGTAAG GAAAGCATTG ATGGTCTTCC AGAGACCCTT 
GTTCTTGAAG ATCACACTGC TATTGGTCAG GAACAATGCT GTCCAATCTG TTGCAGTGAG 
TATATTAAGG ATGATATAGC AACAGAGTTG CCCTGTCACC ATTTCTTTCA CAAACCTTGT 
GTCTCAATTT GGCTACAAAA GTCGGGAACA TGCCCTGTGT GCCGCCGTCA TTTCCCACCT 
GCGGTTATTG AAGCATCTGC AGCTCCTTCC TCTGAGCCTG ATCCTGATGC CCCACCTTCA 
AATGACAGTA TTGCAGAAGC ACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865588.1
CDS	297..2423
Translation	MSQYTEKEPAAMDQESGKAVWPKPAGGYQTITGRRYGRRHAYVSFKPCMTRHERSLGRAGDDYEVLELDDVPKENSSGSSPLDQVDSSLPSEPIFEKSETEIPTCGSALNQTTESSQSFVAVHHSEEGRDTLGSSTNLHNHSEGEYIPGACSASSVQNGIALVHTDSYDPDGKHGEDNDHLQLSAEVVEGSRYQESLGNTVFELENREAEAYTGLSPPVPSFNCEVRDEFEELDSVPLVKSSAGDTEFVHQNSQEIQRSSQDEMVSTKQQNNTSQERQTEHSPEDAACGPGHICSEQNTNDREKNHGSSPEQVVRPKVRKLISSSQVDQETGFNRHEAKQRSVQRWREALEVEESGSDDLLIKCEEYDGEHDCMFLDPPYSRVITQRETENNQMTSESGATAGRQEVDNTFWNGCGDYYQLYDKDEDSSECSDGEWSASLPHRFSGTEKDQSSSDESWETLPGKDENEPELQSDSSGPEEENQELSLQEGEQTSLEEGEIPWLQYNEVNESSSDEGNEPANEFAQPAFMLDGNNNLEDDSSVSEDLDVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLEDHTAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVIEASAAPSSEPDPDAPPSNDSIAEAP

Target ORF information:

RefSeq Version	XM_017010099.1
Organism	Homo sapiens(human)
Definition	Homo sapiens praja ring finger ubiquitin ligase 2 (PJA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010099.1

ORF Insert Sequence:

ATGTCACAGT ACACTGAAAA GGAGCCAGCA GCAATGGACC AAGAATCTGG TAAGGCTGTC 
TGGCCCAAAC CAGCAGGAGG GTATCAGACA ATTACAGGCA GGAGATATGG AAGAAGACAT 
GCTTATGTCA GTTTTAAACC ATGTATGACC AGACATGAAA GAAGCTTAGG TCGGGCTGGT 
GATGACTATG AAGTGTTGGA ACTAGATGAT GTTCCAAAGG AAAATTCCTC AGGTTCCAGT 
CCTTTGGATC AAGTTGATTC TTCTTTACCC AGTGAACCTA TATTTGAAAA AAGTGAAACA 
GAAATTCCCA CTTGTGGTTC AGCATTGAAT CAAACCACTG AGAGCAGTCA ATCCTTTGTT 
GCAGTACATC ACAGTGAGGA AGGCAGGGAT ACCTTAGGAA GCAGTACAAA TCTTCATAAT 
CACTCTGAGG GAGAGTATAT TCCAGGAGCT TGTAGTGCTT CAAGTGTCCA AAATGGAATT 
GCATTGGTTC ATACAGACTC TTATGATCCA GATGGCAAAC ATGGAGAAGA TAATGACCAT 
CTTCAACTTT CTGCAGAAGT CGTGGAAGGT AGTAGATACC AGGAATCATT AGGCAATACA 
GTATTTGAGT TGGAAAACAG AGAGGCAGAG GCATACACTG GTCTTTCACC ACCAGTTCCC 
TCATTTAACT GTGAAGTAAG AGATGAGTTT GAAGAGTTAG ATTCTGTACC ATTAGTGAAA 
AGTTCTGCTG GTGATACTGA GTTTGTCCAT CAGAATAGCC AGGAAATTCA GAGGTCTTCT 
CAAGATGAAA TGGTTAGTAC GAAACAACAA AATAATACTA GCCAGGAAAG ACAGACAGAA 
CATTCACCTG AAGATGCAGC CTGTGGTCCA GGGCATATTT GTAGTGAACA AAATACCAAT 
GATAGGGAAA AGAACCATGG AAGTTCTCCT GAACAGGTAG TGAGGCCAAA AGTTAGAAAA 
CTGATAAGTT CAAGCCAGGT GGACCAAGAA ACAGGTTTTA ATAGGCATGA GGCGAAACAA 
AGAAGTGTTC AAAGATGGAG AGAGGCTTTG GAAGTTGAGG AAAGTGGCTC AGATGACCTC 
TTAATAAAAT GTGAAGAATA TGATGGAGAG CATGACTGTA TGTTCTTGGA TCCACCATAC 
TCAAGAGTTA TTACACAAAG GGAAACAGAA AATAACCAAA TGACATCAGA AAGTGGAGCC 
ACAGCAGGAA GGCAAGAGGT GGATAACACC TTTTGGAATG GCTGTGGAGA TTATTACCAA 
CTCTATGACA AAGATGAAGA TAGTTCTGAA TGCAGTGATG GGGAATGGTC TGCTTCTTTG 
CCTCATCGAT TTTCTGGTAC AGAAAAAGAT CAATCCTCAA GTGATGAAAG CTGGGAGACT 
CTGCCAGGAA AAGATGAGAA TGAACCTGAG CTACAAAGTG ATAGCAGTGG CCCTGAAGAA 
GAAAACCAAG AATTATCTCT TCAGGAAGGG GAACAGACAT CCTTGGAAGA GGGAGAAATT 
CCTTGGTTAC AGTACAATGA AGTCAATGAA AGCAGCAGTG ATGAGGGAAA TGAACCTGCC 
AATGAATTTG CACAGCCAGC TTTCATGTTG GATGGTAACA ATAACCTGGA GGATGACTCC 
AGTGTGAGTG AAGACTTAGA TGTGGATTGG AGCCTATTTG ATGGCTTTGC AGATGGACTA 
GGAGTTGCTG AAGCTATTTC ATATGTGGAT CCTCAGTTCC TTACCTACAT GGCACTAGAA 
GAACGCTTAG CCCAGGCTAT GGAGACTGCT CTGGCCCATT TAGAGTCTCT TGCAGTGGAT 
GTTGAGGTGG CCAATCCACC AGCTAGTAAG GAAAGCATTG ATGGTCTTCC AGAGACCCTT 
GTTCTTGAAG ATCACACTGC TATTGGTCAG GAACAATGCT GTCCAATCTG TTGCAGTGAG 
TATATTAAGG ATGATATAGC AACAGAGTTG CCCTGTCACC ATTTCTTTCA CAAACCTTGT 
GTCTCAATTT GGCTACAAAA GTCGGGAACA TGCCCTGTGT GCCGCCGTCA TTTCCCACCT 
GCGGTTATTG AAGCATCTGC AGCTCCTTCC TCTGAGCCTG ATCCTGATGC CCCACCTTCA 
AATGACAGTA TTGCAGAAGC ACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PJA2 ( XM_017010099.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865588.1 Homo sapiens praja ring finger ubiquitin ligase 2 (PJA2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu17380
Clone ID Related Accession (Same CDS sequence)	NM_014819.5 , NM_014819.4 , XM_017010099.1 , XM_017010099.2
Accession Version	XM_017010099.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase Praja-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017010099.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACAGT ACACTGAAAA GGAGCCAGCA GCAATGGACC AAGAATCTGG TAAGGCTGTC 
TGGCCCAAAC CAGCAGGAGG GTATCAGACA ATTACAGGCA GGAGATATGG AAGAAGACAT 
GCTTATGTCA GTTTTAAACC ATGTATGACC AGACATGAAA GAAGCTTAGG TCGGGCTGGT 
GATGACTATG AAGTGTTGGA ACTAGATGAT GTTCCAAAGG AAAATTCCTC AGGTTCCAGT 
CCTTTGGATC AAGTTGATTC TTCTTTACCC AGTGAACCTA TATTTGAAAA AAGTGAAACA 
GAAATTCCCA CTTGTGGTTC AGCATTGAAT CAAACCACTG AGAGCAGTCA ATCCTTTGTT 
GCAGTACATC ACAGTGAGGA AGGCAGGGAT ACCTTAGGAA GCAGTACAAA TCTTCATAAT 
CACTCTGAGG GAGAGTATAT TCCAGGAGCT TGTAGTGCTT CAAGTGTCCA AAATGGAATT 
GCATTGGTTC ATACAGACTC TTATGATCCA GATGGCAAAC ATGGAGAAGA TAATGACCAT 
CTTCAACTTT CTGCAGAAGT CGTGGAAGGT AGTAGATACC AGGAATCATT AGGCAATACA 
GTATTTGAGT TGGAAAACAG AGAGGCAGAG GCATACACTG GTCTTTCACC ACCAGTTCCC 
TCATTTAACT GTGAAGTAAG AGATGAGTTT GAAGAGTTAG ATTCTGTACC ATTAGTGAAA 
AGTTCTGCTG GTGATACTGA GTTTGTCCAT CAGAATAGCC AGGAAATTCA GAGGTCTTCT 
CAAGATGAAA TGGTTAGTAC GAAACAACAA AATAATACTA GCCAGGAAAG ACAGACAGAA 
CATTCACCTG AAGATGCAGC CTGTGGTCCA GGGCATATTT GTAGTGAACA AAATACCAAT 
GATAGGGAAA AGAACCATGG AAGTTCTCCT GAACAGGTAG TGAGGCCAAA AGTTAGAAAA 
CTGATAAGTT CAAGCCAGGT GGACCAAGAA ACAGGTTTTA ATAGGCATGA GGCGAAACAA 
AGAAGTGTTC AAAGATGGAG AGAGGCTTTG GAAGTTGAGG AAAGTGGCTC AGATGACCTC 
TTAATAAAAT GTGAAGAATA TGATGGAGAG CATGACTGTA TGTTCTTGGA TCCACCATAC 
TCAAGAGTTA TTACACAAAG GGAAACAGAA AATAACCAAA TGACATCAGA AAGTGGAGCC 
ACAGCAGGAA GGCAAGAGGT GGATAACACC TTTTGGAATG GCTGTGGAGA TTATTACCAA 
CTCTATGACA AAGATGAAGA TAGTTCTGAA TGCAGTGATG GGGAATGGTC TGCTTCTTTG 
CCTCATCGAT TTTCTGGTAC AGAAAAAGAT CAATCCTCAA GTGATGAAAG CTGGGAGACT 
CTGCCAGGAA AAGATGAGAA TGAACCTGAG CTACAAAGTG ATAGCAGTGG CCCTGAAGAA 
GAAAACCAAG AATTATCTCT TCAGGAAGGG GAACAGACAT CCTTGGAAGA GGGAGAAATT 
CCTTGGTTAC AGTACAATGA AGTCAATGAA AGCAGCAGTG ATGAGGGAAA TGAACCTGCC 
AATGAATTTG CACAGCCAGC TTTCATGTTG GATGGTAACA ATAACCTGGA GGATGACTCC 
AGTGTGAGTG AAGACTTAGA TGTGGATTGG AGCCTATTTG ATGGCTTTGC AGATGGACTA 
GGAGTTGCTG AAGCTATTTC ATATGTGGAT CCTCAGTTCC TTACCTACAT GGCACTAGAA 
GAACGCTTAG CCCAGGCTAT GGAGACTGCT CTGGCCCATT TAGAGTCTCT TGCAGTGGAT 
GTTGAGGTGG CCAATCCACC AGCTAGTAAG GAAAGCATTG ATGGTCTTCC AGAGACCCTT 
GTTCTTGAAG ATCACACTGC TATTGGTCAG GAACAATGCT GTCCAATCTG TTGCAGTGAG 
TATATTAAGG ATGATATAGC AACAGAGTTG CCCTGTCACC ATTTCTTTCA CAAACCTTGT 
GTCTCAATTT GGCTACAAAA GTCGGGAACA TGCCCTGTGT GCCGCCGTCA TTTCCCACCT 
GCGGTTATTG AAGCATCTGC AGCTCCTTCC TCTGAGCCTG ATCCTGATGC CCCACCTTCA 
AATGACAGTA TTGCAGAAGC ACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865588.1
CDS	346..2472
Translation	MSQYTEKEPAAMDQESGKAVWPKPAGGYQTITGRRYGRRHAYVSFKPCMTRHERSLGRAGDDYEVLELDDVPKENSSGSSPLDQVDSSLPSEPIFEKSETEIPTCGSALNQTTESSQSFVAVHHSEEGRDTLGSSTNLHNHSEGEYIPGACSASSVQNGIALVHTDSYDPDGKHGEDNDHLQLSAEVVEGSRYQESLGNTVFELENREAEAYTGLSPPVPSFNCEVRDEFEELDSVPLVKSSAGDTEFVHQNSQEIQRSSQDEMVSTKQQNNTSQERQTEHSPEDAACGPGHICSEQNTNDREKNHGSSPEQVVRPKVRKLISSSQVDQETGFNRHEAKQRSVQRWREALEVEESGSDDLLIKCEEYDGEHDCMFLDPPYSRVITQRETENNQMTSESGATAGRQEVDNTFWNGCGDYYQLYDKDEDSSECSDGEWSASLPHRFSGTEKDQSSSDESWETLPGKDENEPELQSDSSGPEEENQELSLQEGEQTSLEEGEIPWLQYNEVNESSSDEGNEPANEFAQPAFMLDGNNNLEDDSSVSEDLDVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLEDHTAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVIEASAAPSSEPDPDAPPSNDSIAEAP

Target ORF information:

RefSeq Version	XM_017010099.2
Organism	Homo sapiens(human)
Definition	Homo sapiens praja ring finger ubiquitin ligase 2 (PJA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010099.2

ORF Insert Sequence:

ATGTCACAGT ACACTGAAAA GGAGCCAGCA GCAATGGACC AAGAATCTGG TAAGGCTGTC 
TGGCCCAAAC CAGCAGGAGG GTATCAGACA ATTACAGGCA GGAGATATGG AAGAAGACAT 
GCTTATGTCA GTTTTAAACC ATGTATGACC AGACATGAAA GAAGCTTAGG TCGGGCTGGT 
GATGACTATG AAGTGTTGGA ACTAGATGAT GTTCCAAAGG AAAATTCCTC AGGTTCCAGT 
CCTTTGGATC AAGTTGATTC TTCTTTACCC AGTGAACCTA TATTTGAAAA AAGTGAAACA 
GAAATTCCCA CTTGTGGTTC AGCATTGAAT CAAACCACTG AGAGCAGTCA ATCCTTTGTT 
GCAGTACATC ACAGTGAGGA AGGCAGGGAT ACCTTAGGAA GCAGTACAAA TCTTCATAAT 
CACTCTGAGG GAGAGTATAT TCCAGGAGCT TGTAGTGCTT CAAGTGTCCA AAATGGAATT 
GCATTGGTTC ATACAGACTC TTATGATCCA GATGGCAAAC ATGGAGAAGA TAATGACCAT 
CTTCAACTTT CTGCAGAAGT CGTGGAAGGT AGTAGATACC AGGAATCATT AGGCAATACA 
GTATTTGAGT TGGAAAACAG AGAGGCAGAG GCATACACTG GTCTTTCACC ACCAGTTCCC 
TCATTTAACT GTGAAGTAAG AGATGAGTTT GAAGAGTTAG ATTCTGTACC ATTAGTGAAA 
AGTTCTGCTG GTGATACTGA GTTTGTCCAT CAGAATAGCC AGGAAATTCA GAGGTCTTCT 
CAAGATGAAA TGGTTAGTAC GAAACAACAA AATAATACTA GCCAGGAAAG ACAGACAGAA 
CATTCACCTG AAGATGCAGC CTGTGGTCCA GGGCATATTT GTAGTGAACA AAATACCAAT 
GATAGGGAAA AGAACCATGG AAGTTCTCCT GAACAGGTAG TGAGGCCAAA AGTTAGAAAA 
CTGATAAGTT CAAGCCAGGT GGACCAAGAA ACAGGTTTTA ATAGGCATGA GGCGAAACAA 
AGAAGTGTTC AAAGATGGAG AGAGGCTTTG GAAGTTGAGG AAAGTGGCTC AGATGACCTC 
TTAATAAAAT GTGAAGAATA TGATGGAGAG CATGACTGTA TGTTCTTGGA TCCACCATAC 
TCAAGAGTTA TTACACAAAG GGAAACAGAA AATAACCAAA TGACATCAGA AAGTGGAGCC 
ACAGCAGGAA GGCAAGAGGT GGATAACACC TTTTGGAATG GCTGTGGAGA TTATTACCAA 
CTCTATGACA AAGATGAAGA TAGTTCTGAA TGCAGTGATG GGGAATGGTC TGCTTCTTTG 
CCTCATCGAT TTTCTGGTAC AGAAAAAGAT CAATCCTCAA GTGATGAAAG CTGGGAGACT 
CTGCCAGGAA AAGATGAGAA TGAACCTGAG CTACAAAGTG ATAGCAGTGG CCCTGAAGAA 
GAAAACCAAG AATTATCTCT TCAGGAAGGG GAACAGACAT CCTTGGAAGA GGGAGAAATT 
CCTTGGTTAC AGTACAATGA AGTCAATGAA AGCAGCAGTG ATGAGGGAAA TGAACCTGCC 
AATGAATTTG CACAGCCAGC TTTCATGTTG GATGGTAACA ATAACCTGGA GGATGACTCC 
AGTGTGAGTG AAGACTTAGA TGTGGATTGG AGCCTATTTG ATGGCTTTGC AGATGGACTA 
GGAGTTGCTG AAGCTATTTC ATATGTGGAT CCTCAGTTCC TTACCTACAT GGCACTAGAA 
GAACGCTTAG CCCAGGCTAT GGAGACTGCT CTGGCCCATT TAGAGTCTCT TGCAGTGGAT 
GTTGAGGTGG CCAATCCACC AGCTAGTAAG GAAAGCATTG ATGGTCTTCC AGAGACCCTT 
GTTCTTGAAG ATCACACTGC TATTGGTCAG GAACAATGCT GTCCAATCTG TTGCAGTGAG 
TATATTAAGG ATGATATAGC AACAGAGTTG CCCTGTCACC ATTTCTTTCA CAAACCTTGT 
GTCTCAATTT GGCTACAAAA GTCGGGAACA TGCCCTGTGT GCCGCCGTCA TTTCCCACCT 
GCGGTTATTG AAGCATCTGC AGCTCCTTCC TCTGAGCCTG ATCCTGATGC CCCACCTTCA 
AATGACAGTA TTGCAGAAGC ACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ubiquitylation of MFHAS1 by the ubiquitin ligase praja2 promotes M1 macrophage polarization by activating JNK and p38 pathways.
Cell death & disease8(5)e2763(2017 May)
Zhong J,Wang H,Chen W,Sun Z,Chen J,Xu Y,Weng M,Shi Q,Ma D,Miao C

PJA2 ubiquitinates the HIV-1 Tat protein with atypical chain linkages to activate viral transcription.
Scientific reports745394(2017 Mar)
Faust TB,Li Y,Jang GM,Johnson JR,Yang S,Weiss A,Krogan NJ,Frankel AD

praja2 regulates KSR1 stability and mitogenic signaling.
Cell death & disease7e2230(2016 May)
Rinaldi L,Delle Donne R,Sepe M,Porpora M,Garbi C,Chiuso F,Gallo A,Parisi S,Russo L,Bachmann V,Huber RG,Stefan E,Russo T,Feliciello A

Detection of novel paraja ring finger 2-fer tyrosine kinase mRNA chimeras is associated with poor postoperative prognosis in non-small cell lung cancer.
Cancer science104(11)1447-54(2013 Nov)
Kawakami M,Ishikawa R,Amano Y,Sunohara M,Watanabe K,Ohishi N,Yatomi Y,Nakajima J,Fukayama M,Nagase T,Takai D

Proteolysis of MOB1 by the ubiquitin ligase praja2 attenuates Hippo signalling and supports glioblastoma growth.
Nature communications41822(2013)
Lignitto L,Arcella A,Sepe M,Rinaldi L,Delle Donne R,Gallo A,Stefan E,Bachmann VA,Oliva MA,Tiziana Storlazzi C,L'Abbate A,Brunetti A,Gargiulo S,Gramanzini M,Insabato L,Garbi C,Gottesman ME,Feliciello A

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PJA2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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