pja2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following pja2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pja2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa03446 XM_004910463.2
Latest version!
Xenopus tropicalis praja ring finger 2, E3 ubiquitin protein ligase (pja2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa03446 XM_002935473.5
Latest version!
Xenopus tropicalis praja ring finger 2, E3 ubiquitin protein ligase (pja2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa32881 XM_004910462.4
Latest version!
Xenopus tropicalis praja ring finger 2, E3 ubiquitin protein ligase (pja2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa42146 XM_004910462.3
Latest version!
Xenopus tropicalis praja ring finger 2, E3 ubiquitin protein ligase (pja2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OXa42147 XM_018089900.1
Latest version!
Xenopus tropicalis praja ring finger 2, E3 ubiquitin protein ligase (pja2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OXa49062 XM_018089900.2
Latest version!
Xenopus tropicalis praja ring finger 2, E3 ubiquitin protein ligase (pja2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OXa03446 XM_002935473.4 Xenopus tropicalis praja ring finger 2, E3 ubiquitin protein ligase (pja2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa03446
    Clone ID Related Accession (Same CDS sequence) XM_002935473.5 , XM_004910463.2 , XM_002935473.4
    Accession Version XM_004910463.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase Praja-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_004910463.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGTCAGG AAGCATGCAA AGCAGCCTGG CCCAAGTCAG CTGGAGGCTC TCAGACCATC 
    ACTGGGCGGA GGTATGGAAG GAGGCATGCA TATGTGAACT TTCGGCCTTC TTTAAAGAGC
    CCAAAAAGAG CATCAAGTCA ACAAATCAAA GAGTGCGAAG GACTGGAACT TGATGATGTA
    CAGACAGAAA AAGATTTAAT TTCCATGGAA AGCTGTGATT CACCTCTGTA CGACATTAGT
    AAATTTTTAA CGACTGACTT GCAACAAGAC AACAATAAAC TGAAAGCAAT GGATGATGTT
    ACATCTTTAG TTACAGAAGT AGAGGAACAT GGAGTTTGCC AATCCTTATG TAATAACAAT
    ATCTGTAAAA AAAACAAGAC TCTTACTAGG CATGTACCCC ATAAGTATCA AAATGGAATT
    TCCTTTTTAA ATATTGACTC TTTTGAGCCA GACAGTAGTG AAGGAGAGGA AAACTGTTAC
    AGTTCAGAGG ATTTTACGTT GACTGAGGAG ACCGGCAAAA TTGAAAAATC CTTAGAAAAC
    ACACTATCTG AGCTTGAGGA GGTGGGGACA TTTTGTGAAA TGGAGCCTGA GTTACAAAGT
    AAAAATCACA GTTGTGCAAA AGACAGATTA TCAGAGATGT GGCCTGTATC TTTATGCAAA
    AGTTCCAAAA TTGATTTTGG GACCACCCAT GGCAAATGGA ATGTGTCAGT AAAATCTGTT
    TCAGAGGATG AGGCCACAGC ACTGGGTATT TCTGAATTCG ATTCAAAGAA AAGTAGGACT
    GATATAACAT TAAAAAATTC TGTTGGACTC TATCCTGAAA CTGCTTCTAA TAGCACTACA
    ATGGAGCAAG ATAGTTTAGC TGAACCAGTT GTCAGACCAA AAGTTAGAAA GCAAGGTACA
    AGTTATGGCA ATAAAAACTC TTCCCTACCG GAAGAAAGCA AGGACAATTC AACAGTCCGA
    AAGAGCAATC GAGGAACACG AGTCAAAGTG GAAAATTCCT GCAAAGATGA AAAGAGTTAC
    CCTGAAATGG AGTCTTCTGG CAGTAATGAT GAGGGCACAA GAAAAAAAAT CAAGAGCATT
    TGCAAAGAGC AAGAATATAC CGACTGCCAT GTTGACAAAC CTGCAGTGGA TGATACATTT
    TGGGATGACT TTGAAATATA TGGAATGAAA TTGTCTGGCT CCATTAAGGA CGATGAAAGC
    TCTGTCTGCA GTGATGGAGA ATGGTCTACA TGCTTGCCTT CATATTTTTC TTCGGACAAA
    GACCATTCGT CAAGTGATGA TAGCTGGGAA ACACTGCCTG GAAAAGATGA ACACGAAGTT
    CTTAGTAACA GCAGTAGTCT GGAAGAAGAA AATTCTGACT TTTGTTTTCA AGTAGAGGAA
    CAAATTTCCT TGGAAGATGG AGAAATCCCT TGGTTGCAGT ACCATGAGGA TATTGAAAGC
    AGCACCGATG AAGAAAATGA AATAAGTAAC CAGTTTGTGC ATCCTGGATT TTTCATGTTA
    GATGGCAATA ACAATTTGGA GGATGATTCA AGTATCAGTG AAGACCTGGA AGTTGAATGG
    AGATTGCTTG ATGATTTTGG TGATGGACTA GGAGTTGCAC AAGCCATGTC CTATATGGAC
    CCTCAGTTTC TTACATATAT GGCTCTTGAG GAGCGCCTTG CCCAGGCTAT GGAGACTGCT
    CTAGCACATT TGGAATCACT TGCCGTTGAT GTAGAACAGG CTCACCCACC AGCTACTAAA
    GAAAGCATAG ACTGCCTCCC TCAGATCATT ATTGGTGAAG ACCATAATAT CGTAGGTCAA
    GAGCAGTGCT GTGCCATCTG CTGCAGTGAG TATATCAAAG ATGAAATATT AACTGAACTA
    CCATGTCACC ACCTTTTCCA TAAGCCCTGT GTTACACTTT GGCTACAGAA ATCTGGGACG
    TGCCCTGTTT GTCGTCACGT GCTTGCATCT TCACACACTG ACGCCGCTGC AACATCTTTC
    CTGTCTGATC ACGAGAGCCC CCCTTCAATC CACAGTGCAG CAGGAACCCG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910520.1
    CDS93..2126
    Translation

    Target ORF information:

    RefSeq Version XM_004910463.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis praja ring finger 2, E3 ubiquitin protein ligase (pja2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910463.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGTCAGG AAGCATGCAA AGCAGCCTGG CCCAAGTCAG CTGGAGGCTC TCAGACCATC 
    ACTGGGCGGA GGTATGGAAG GAGGCATGCA TATGTGAACT TTCGGCCTTC TTTAAAGAGC
    CCAAAAAGAG CATCAAGTCA ACAAATCAAA GAGTGCGAAG GACTGGAACT TGATGATGTA
    CAGACAGAAA AAGATTTAAT TTCCATGGAA AGCTGTGATT CACCTCTGTA CGACATTAGT
    AAATTTTTAA CGACTGACTT GCAACAAGAC AACAATAAAC TGAAAGCAAT GGATGATGTT
    ACATCTTTAG TTACAGAAGT AGAGGAACAT GGAGTTTGCC AATCCTTATG TAATAACAAT
    ATCTGTAAAA AAAACAAGAC TCTTACTAGG CATGTACCCC ATAAGTATCA AAATGGAATT
    TCCTTTTTAA ATATTGACTC TTTTGAGCCA GACAGTAGTG AAGGAGAGGA AAACTGTTAC
    AGTTCAGAGG ATTTTACGTT GACTGAGGAG ACCGGCAAAA TTGAAAAATC CTTAGAAAAC
    ACACTATCTG AGCTTGAGGA GGTGGGGACA TTTTGTGAAA TGGAGCCTGA GTTACAAAGT
    AAAAATCACA GTTGTGCAAA AGACAGATTA TCAGAGATGT GGCCTGTATC TTTATGCAAA
    AGTTCCAAAA TTGATTTTGG GACCACCCAT GGCAAATGGA ATGTGTCAGT AAAATCTGTT
    TCAGAGGATG AGGCCACAGC ACTGGGTATT TCTGAATTCG ATTCAAAGAA AAGTAGGACT
    GATATAACAT TAAAAAATTC TGTTGGACTC TATCCTGAAA CTGCTTCTAA TAGCACTACA
    ATGGAGCAAG ATAGTTTAGC TGAACCAGTT GTCAGACCAA AAGTTAGAAA GCAAGGTACA
    AGTTATGGCA ATAAAAACTC TTCCCTACCG GAAGAAAGCA AGGACAATTC AACAGTCCGA
    AAGAGCAATC GAGGAACACG AGTCAAAGTG GAAAATTCCT GCAAAGATGA AAAGAGTTAC
    CCTGAAATGG AGTCTTCTGG CAGTAATGAT GAGGGCACAA GAAAAAAAAT CAAGAGCATT
    TGCAAAGAGC AAGAATATAC CGACTGCCAT GTTGACAAAC CTGCAGTGGA TGATACATTT
    TGGGATGACT TTGAAATATA TGGAATGAAA TTGTCTGGCT CCATTAAGGA CGATGAAAGC
    TCTGTCTGCA GTGATGGAGA ATGGTCTACA TGCTTGCCTT CATATTTTTC TTCGGACAAA
    GACCATTCGT CAAGTGATGA TAGCTGGGAA ACACTGCCTG GAAAAGATGA ACACGAAGTT
    CTTAGTAACA GCAGTAGTCT GGAAGAAGAA AATTCTGACT TTTGTTTTCA AGTAGAGGAA
    CAAATTTCCT TGGAAGATGG AGAAATCCCT TGGTTGCAGT ACCATGAGGA TATTGAAAGC
    AGCACCGATG AAGAAAATGA AATAAGTAAC CAGTTTGTGC ATCCTGGATT TTTCATGTTA
    GATGGCAATA ACAATTTGGA GGATGATTCA AGTATCAGTG AAGACCTGGA AGTTGAATGG
    AGATTGCTTG ATGATTTTGG TGATGGACTA GGAGTTGCAC AAGCCATGTC CTATATGGAC
    CCTCAGTTTC TTACATATAT GGCTCTTGAG GAGCGCCTTG CCCAGGCTAT GGAGACTGCT
    CTAGCACATT TGGAATCACT TGCCGTTGAT GTAGAACAGG CTCACCCACC AGCTACTAAA
    GAAAGCATAG ACTGCCTCCC TCAGATCATT ATTGGTGAAG ACCATAATAT CGTAGGTCAA
    GAGCAGTGCT GTGCCATCTG CTGCAGTGAG TATATCAAAG ATGAAATATT AACTGAACTA
    CCATGTCACC ACCTTTTCCA TAAGCCCTGT GTTACACTTT GGCTACAGAA ATCTGGGACG
    TGCCCTGTTT GTCGTCACGT GCTTGCATCT TCACACACTG ACGCCGCTGC AACATCTTTC
    CTGTCTGATC ACGAGAGCCC CCCTTCAATC CACAGTGCAG CAGGAACCCG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03446
    Clone ID Related Accession (Same CDS sequence) XM_002935473.5 , XM_004910463.2 , XM_002935473.4
    Accession Version XM_002935473.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase Praja-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002935473.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGTCAGG AAGCATGCAA AGCAGCCTGG CCCAAGTCAG CTGGAGGCTC TCAGACCATC 
    ACTGGGCGGA GGTATGGAAG GAGGCATGCA TATGTGAACT TTCGGCCTTC TTTAAAGAGC
    CCAAAAAGAG CATCAAGTCA ACAAATCAAA GAGTGCGAAG GACTGGAACT TGATGATGTA
    CAGACAGAAA AAGATTTAAT TTCCATGGAA AGCTGTGATT CACCTCTGTA CGACATTAGT
    AAATTTTTAA CGACTGACTT GCAACAAGAC AACAATAAAC TGAAAGCAAT GGATGATGTT
    ACATCTTTAG TTACAGAAGT AGAGGAACAT GGAGTTTGCC AATCCTTATG TAATAACAAT
    ATCTGTAAAA AAAACAAGAC TCTTACTAGG CATGTACCCC ATAAGTATCA AAATGGAATT
    TCCTTTTTAA ATATTGACTC TTTTGAGCCA GACAGTAGTG AAGGAGAGGA AAACTGTTAC
    AGTTCAGAGG ATTTTACGTT GACTGAGGAG ACCGGCAAAA TTGAAAAATC CTTAGAAAAC
    ACACTATCTG AGCTTGAGGA GGTGGGGACA TTTTGTGAAA TGGAGCCTGA GTTACAAAGT
    AAAAATCACA GTTGTGCAAA AGACAGATTA TCAGAGATGT GGCCTGTATC TTTATGCAAA
    AGTTCCAAAA TTGATTTTGG GACCACCCAT GGCAAATGGA ATGTGTCAGT AAAATCTGTT
    TCAGAGGATG AGGCCACAGC ACTGGGTATT TCTGAATTCG ATTCAAAGAA AAGTAGGACT
    GATATAACAT TAAAAAATTC TGTTGGACTC TATCCTGAAA CTGCTTCTAA TAGCACTACA
    ATGGAGCAAG ATAGTTTAGC TGAACCAGTT GTCAGACCAA AAGTTAGAAA GCAAGGTACA
    AGTTATGGCA ATAAAAACTC TTCCCTACCG GAAGAAAGCA AGGACAATTC AACAGTCCGA
    AAGAGCAATC GAGGAACACG AGTCAAAGTG GAAAATTCCT GCAAAGATGA AAAGAGTTAC
    CCTGAAATGG AGTCTTCTGG CAGTAATGAT GAGGGCACAA GAAAAAAAAT CAAGAGCATT
    TGCAAAGAGC AAGAATATAC CGACTGCCAT GTTGACAAAC CTGCAGTGGA TGATACATTT
    TGGGATGACT TTGAAATATA TGGAATGAAA TTGTCTGGCT CCATTAAGGA CGATGAAAGC
    TCTGTCTGCA GTGATGGAGA ATGGTCTACA TGCTTGCCTT CATATTTTTC TTCGGACAAA
    GACCATTCGT CAAGTGATGA TAGCTGGGAA ACACTGCCTG GAAAAGATGA ACACGAAGTT
    CTTAGTAACA GCAGTAGTCT GGAAGAAGAA AATTCTGACT TTTGTTTTCA AGTAGAGGAA
    CAAATTTCCT TGGAAGATGG AGAAATCCCT TGGTTGCAGT ACCATGAGGA TATTGAAAGC
    AGCACCGATG AAGAAAATGA AATAAGTAAC CAGTTTGTGC ATCCTGGATT TTTCATGTTA
    GATGGCAATA ACAATTTGGA GGATGATTCA AGTATCAGTG AAGACCTGGA AGTTGAATGG
    AGATTGCTTG ATGATTTTGG TGATGGACTA GGAGTTGCAC AAGCCATGTC CTATATGGAC
    CCTCAGTTTC TTACATATAT GGCTCTTGAG GAGCGCCTTG CCCAGGCTAT GGAGACTGCT
    CTAGCACATT TGGAATCACT TGCCGTTGAT GTAGAACAGG CTCACCCACC AGCTACTAAA
    GAAAGCATAG ACTGCCTCCC TCAGATCATT ATTGGTGAAG ACCATAATAT CGTAGGTCAA
    GAGCAGTGCT GTGCCATCTG CTGCAGTGAG TATATCAAAG ATGAAATATT AACTGAACTA
    CCATGTCACC ACCTTTTCCA TAAGCCCTGT GTTACACTTT GGCTACAGAA ATCTGGGACG
    TGCCCTGTTT GTCGTCACGT GCTTGCATCT TCACACACTG ACGCCGCTGC AACATCTTTC
    CTGTCTGATC ACGAGAGCCC CCCTTCAATC CACAGTGCAG CAGGAACCCG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002935519.1
    CDS352..2385
    Translation

    Target ORF information:

    RefSeq Version XM_002935473.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis praja ring finger 2, E3 ubiquitin protein ligase (pja2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002935473.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGTCAGG AAGCATGCAA AGCAGCCTGG CCCAAGTCAG CTGGAGGCTC TCAGACCATC 
    ACTGGGCGGA GGTATGGAAG GAGGCATGCA TATGTGAACT TTCGGCCTTC TTTAAAGAGC
    CCAAAAAGAG CATCAAGTCA ACAAATCAAA GAGTGCGAAG GACTGGAACT TGATGATGTA
    CAGACAGAAA AAGATTTAAT TTCCATGGAA AGCTGTGATT CACCTCTGTA CGACATTAGT
    AAATTTTTAA CGACTGACTT GCAACAAGAC AACAATAAAC TGAAAGCAAT GGATGATGTT
    ACATCTTTAG TTACAGAAGT AGAGGAACAT GGAGTTTGCC AATCCTTATG TAATAACAAT
    ATCTGTAAAA AAAACAAGAC TCTTACTAGG CATGTACCCC ATAAGTATCA AAATGGAATT
    TCCTTTTTAA ATATTGACTC TTTTGAGCCA GACAGTAGTG AAGGAGAGGA AAACTGTTAC
    AGTTCAGAGG ATTTTACGTT GACTGAGGAG ACCGGCAAAA TTGAAAAATC CTTAGAAAAC
    ACACTATCTG AGCTTGAGGA GGTGGGGACA TTTTGTGAAA TGGAGCCTGA GTTACAAAGT
    AAAAATCACA GTTGTGCAAA AGACAGATTA TCAGAGATGT GGCCTGTATC TTTATGCAAA
    AGTTCCAAAA TTGATTTTGG GACCACCCAT GGCAAATGGA ATGTGTCAGT AAAATCTGTT
    TCAGAGGATG AGGCCACAGC ACTGGGTATT TCTGAATTCG ATTCAAAGAA AAGTAGGACT
    GATATAACAT TAAAAAATTC TGTTGGACTC TATCCTGAAA CTGCTTCTAA TAGCACTACA
    ATGGAGCAAG ATAGTTTAGC TGAACCAGTT GTCAGACCAA AAGTTAGAAA GCAAGGTACA
    AGTTATGGCA ATAAAAACTC TTCCCTACCG GAAGAAAGCA AGGACAATTC AACAGTCCGA
    AAGAGCAATC GAGGAACACG AGTCAAAGTG GAAAATTCCT GCAAAGATGA AAAGAGTTAC
    CCTGAAATGG AGTCTTCTGG CAGTAATGAT GAGGGCACAA GAAAAAAAAT CAAGAGCATT
    TGCAAAGAGC AAGAATATAC CGACTGCCAT GTTGACAAAC CTGCAGTGGA TGATACATTT
    TGGGATGACT TTGAAATATA TGGAATGAAA TTGTCTGGCT CCATTAAGGA CGATGAAAGC
    TCTGTCTGCA GTGATGGAGA ATGGTCTACA TGCTTGCCTT CATATTTTTC TTCGGACAAA
    GACCATTCGT CAAGTGATGA TAGCTGGGAA ACACTGCCTG GAAAAGATGA ACACGAAGTT
    CTTAGTAACA GCAGTAGTCT GGAAGAAGAA AATTCTGACT TTTGTTTTCA AGTAGAGGAA
    CAAATTTCCT TGGAAGATGG AGAAATCCCT TGGTTGCAGT ACCATGAGGA TATTGAAAGC
    AGCACCGATG AAGAAAATGA AATAAGTAAC CAGTTTGTGC ATCCTGGATT TTTCATGTTA
    GATGGCAATA ACAATTTGGA GGATGATTCA AGTATCAGTG AAGACCTGGA AGTTGAATGG
    AGATTGCTTG ATGATTTTGG TGATGGACTA GGAGTTGCAC AAGCCATGTC CTATATGGAC
    CCTCAGTTTC TTACATATAT GGCTCTTGAG GAGCGCCTTG CCCAGGCTAT GGAGACTGCT
    CTAGCACATT TGGAATCACT TGCCGTTGAT GTAGAACAGG CTCACCCACC AGCTACTAAA
    GAAAGCATAG ACTGCCTCCC TCAGATCATT ATTGGTGAAG ACCATAATAT CGTAGGTCAA
    GAGCAGTGCT GTGCCATCTG CTGCAGTGAG TATATCAAAG ATGAAATATT AACTGAACTA
    CCATGTCACC ACCTTTTCCA TAAGCCCTGT GTTACACTTT GGCTACAGAA ATCTGGGACG
    TGCCCTGTTT GTCGTCACGT GCTTGCATCT TCACACACTG ACGCCGCTGC AACATCTTTC
    CTGTCTGATC ACGAGAGCCC CCCTTCAATC CACAGTGCAG CAGGAACCCG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03446
    Clone ID Related Accession (Same CDS sequence) XM_002935473.5 , XM_004910463.2 , XM_002935473.4
    Accession Version XM_002935473.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase Praja-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002935473.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGTCAGG AAGCATGCAA AGCAGCCTGG CCCAAGTCAG CTGGAGGCTC TCAGACCATC 
    ACTGGGCGGA GGTATGGAAG GAGGCATGCA TATGTGAACT TTCGGCCTTC TTTAAAGAGC
    CCAAAAAGAG CATCAAGTCA ACAAATCAAA GAGTGCGAAG GACTGGAACT TGATGATGTA
    CAGACAGAAA AAGATTTAAT TTCCATGGAA AGCTGTGATT CACCTCTGTA CGACATTAGT
    AAATTTTTAA CGACTGACTT GCAACAAGAC AACAATAAAC TGAAAGCAAT GGATGATGTT
    ACATCTTTAG TTACAGAAGT AGAGGAACAT GGAGTTTGCC AATCCTTATG TAATAACAAT
    ATCTGTAAAA AAAACAAGAC TCTTACTAGG CATGTACCCC ATAAGTATCA AAATGGAATT
    TCCTTTTTAA ATATTGACTC TTTTGAGCCA GACAGTAGTG AAGGAGAGGA AAACTGTTAC
    AGTTCAGAGG ATTTTACGTT GACTGAGGAG ACCGGCAAAA TTGAAAAATC CTTAGAAAAC
    ACACTATCTG AGCTTGAGGA GGTGGGGACA TTTTGTGAAA TGGAGCCTGA GTTACAAAGT
    AAAAATCACA GTTGTGCAAA AGACAGATTA TCAGAGATGT GGCCTGTATC TTTATGCAAA
    AGTTCCAAAA TTGATTTTGG GACCACCCAT GGCAAATGGA ATGTGTCAGT AAAATCTGTT
    TCAGAGGATG AGGCCACAGC ACTGGGTATT TCTGAATTCG ATTCAAAGAA AAGTAGGACT
    GATATAACAT TAAAAAATTC TGTTGGACTC TATCCTGAAA CTGCTTCTAA TAGCACTACA
    ATGGAGCAAG ATAGTTTAGC TGAACCAGTT GTCAGACCAA AAGTTAGAAA GCAAGGTACA
    AGTTATGGCA ATAAAAACTC TTCCCTACCG GAAGAAAGCA AGGACAATTC AACAGTCCGA
    AAGAGCAATC GAGGAACACG AGTCAAAGTG GAAAATTCCT GCAAAGATGA AAAGAGTTAC
    CCTGAAATGG AGTCTTCTGG CAGTAATGAT GAGGGCACAA GAAAAAAAAT CAAGAGCATT
    TGCAAAGAGC AAGAATATAC CGACTGCCAT GTTGACAAAC CTGCAGTGGA TGATACATTT
    TGGGATGACT TTGAAATATA TGGAATGAAA TTGTCTGGCT CCATTAAGGA CGATGAAAGC
    TCTGTCTGCA GTGATGGAGA ATGGTCTACA TGCTTGCCTT CATATTTTTC TTCGGACAAA
    GACCATTCGT CAAGTGATGA TAGCTGGGAA ACACTGCCTG GAAAAGATGA ACACGAAGTT
    CTTAGTAACA GCAGTAGTCT GGAAGAAGAA AATTCTGACT TTTGTTTTCA AGTAGAGGAA
    CAAATTTCCT TGGAAGATGG AGAAATCCCT TGGTTGCAGT ACCATGAGGA TATTGAAAGC
    AGCACCGATG AAGAAAATGA AATAAGTAAC CAGTTTGTGC ATCCTGGATT TTTCATGTTA
    GATGGCAATA ACAATTTGGA GGATGATTCA AGTATCAGTG AAGACCTGGA AGTTGAATGG
    AGATTGCTTG ATGATTTTGG TGATGGACTA GGAGTTGCAC AAGCCATGTC CTATATGGAC
    CCTCAGTTTC TTACATATAT GGCTCTTGAG GAGCGCCTTG CCCAGGCTAT GGAGACTGCT
    CTAGCACATT TGGAATCACT TGCCGTTGAT GTAGAACAGG CTCACCCACC AGCTACTAAA
    GAAAGCATAG ACTGCCTCCC TCAGATCATT ATTGGTGAAG ACCATAATAT CGTAGGTCAA
    GAGCAGTGCT GTGCCATCTG CTGCAGTGAG TATATCAAAG ATGAAATATT AACTGAACTA
    CCATGTCACC ACCTTTTCCA TAAGCCCTGT GTTACACTTT GGCTACAGAA ATCTGGGACG
    TGCCCTGTTT GTCGTCACGT GCTTGCATCT TCACACACTG ACGCCGCTGC AACATCTTTC
    CTGTCTGATC ACGAGAGCCC CCCTTCAATC CACAGTGCAG CAGGAACCCG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002935519.1
    CDS215..2248
    Translation

    Target ORF information:

    RefSeq Version XM_002935473.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis praja ring finger 2, E3 ubiquitin protein ligase (pja2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002935473.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGTCAGG AAGCATGCAA AGCAGCCTGG CCCAAGTCAG CTGGAGGCTC TCAGACCATC 
    ACTGGGCGGA GGTATGGAAG GAGGCATGCA TATGTGAACT TTCGGCCTTC TTTAAAGAGC
    CCAAAAAGAG CATCAAGTCA ACAAATCAAA GAGTGCGAAG GACTGGAACT TGATGATGTA
    CAGACAGAAA AAGATTTAAT TTCCATGGAA AGCTGTGATT CACCTCTGTA CGACATTAGT
    AAATTTTTAA CGACTGACTT GCAACAAGAC AACAATAAAC TGAAAGCAAT GGATGATGTT
    ACATCTTTAG TTACAGAAGT AGAGGAACAT GGAGTTTGCC AATCCTTATG TAATAACAAT
    ATCTGTAAAA AAAACAAGAC TCTTACTAGG CATGTACCCC ATAAGTATCA AAATGGAATT
    TCCTTTTTAA ATATTGACTC TTTTGAGCCA GACAGTAGTG AAGGAGAGGA AAACTGTTAC
    AGTTCAGAGG ATTTTACGTT GACTGAGGAG ACCGGCAAAA TTGAAAAATC CTTAGAAAAC
    ACACTATCTG AGCTTGAGGA GGTGGGGACA TTTTGTGAAA TGGAGCCTGA GTTACAAAGT
    AAAAATCACA GTTGTGCAAA AGACAGATTA TCAGAGATGT GGCCTGTATC TTTATGCAAA
    AGTTCCAAAA TTGATTTTGG GACCACCCAT GGCAAATGGA ATGTGTCAGT AAAATCTGTT
    TCAGAGGATG AGGCCACAGC ACTGGGTATT TCTGAATTCG ATTCAAAGAA AAGTAGGACT
    GATATAACAT TAAAAAATTC TGTTGGACTC TATCCTGAAA CTGCTTCTAA TAGCACTACA
    ATGGAGCAAG ATAGTTTAGC TGAACCAGTT GTCAGACCAA AAGTTAGAAA GCAAGGTACA
    AGTTATGGCA ATAAAAACTC TTCCCTACCG GAAGAAAGCA AGGACAATTC AACAGTCCGA
    AAGAGCAATC GAGGAACACG AGTCAAAGTG GAAAATTCCT GCAAAGATGA AAAGAGTTAC
    CCTGAAATGG AGTCTTCTGG CAGTAATGAT GAGGGCACAA GAAAAAAAAT CAAGAGCATT
    TGCAAAGAGC AAGAATATAC CGACTGCCAT GTTGACAAAC CTGCAGTGGA TGATACATTT
    TGGGATGACT TTGAAATATA TGGAATGAAA TTGTCTGGCT CCATTAAGGA CGATGAAAGC
    TCTGTCTGCA GTGATGGAGA ATGGTCTACA TGCTTGCCTT CATATTTTTC TTCGGACAAA
    GACCATTCGT CAAGTGATGA TAGCTGGGAA ACACTGCCTG GAAAAGATGA ACACGAAGTT
    CTTAGTAACA GCAGTAGTCT GGAAGAAGAA AATTCTGACT TTTGTTTTCA AGTAGAGGAA
    CAAATTTCCT TGGAAGATGG AGAAATCCCT TGGTTGCAGT ACCATGAGGA TATTGAAAGC
    AGCACCGATG AAGAAAATGA AATAAGTAAC CAGTTTGTGC ATCCTGGATT TTTCATGTTA
    GATGGCAATA ACAATTTGGA GGATGATTCA AGTATCAGTG AAGACCTGGA AGTTGAATGG
    AGATTGCTTG ATGATTTTGG TGATGGACTA GGAGTTGCAC AAGCCATGTC CTATATGGAC
    CCTCAGTTTC TTACATATAT GGCTCTTGAG GAGCGCCTTG CCCAGGCTAT GGAGACTGCT
    CTAGCACATT TGGAATCACT TGCCGTTGAT GTAGAACAGG CTCACCCACC AGCTACTAAA
    GAAAGCATAG ACTGCCTCCC TCAGATCATT ATTGGTGAAG ACCATAATAT CGTAGGTCAA
    GAGCAGTGCT GTGCCATCTG CTGCAGTGAG TATATCAAAG ATGAAATATT AACTGAACTA
    CCATGTCACC ACCTTTTCCA TAAGCCCTGT GTTACACTTT GGCTACAGAA ATCTGGGACG
    TGCCCTGTTT GTCGTCACGT GCTTGCATCT TCACACACTG ACGCCGCTGC AACATCTTTC
    CTGTCTGATC ACGAGAGCCC CCCTTCAATC CACAGTGCAG CAGGAACCCG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa32881
    Clone ID Related Accession (Same CDS sequence) XM_004910462.4
    Accession Version XM_004910462.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2184bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase Praja-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910462.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGAGCTG CGGGCCTGGG TTACCTGTCC GGAAGGAATC GAGGAGCTAC ATATAAACGG 
    CACTGCGAGA CTTTGAAACA GCGATCCGGG GCTGCGGGCT GTGTAAGGAA AAGCGGGACT
    GTCCCGGCCA AAGCGCCACT ATTGGGCGTG ATGGGTCAGG AAGCATGCAA AGCAGCCTGG
    CCCAAGTCAG CTGGAGGCTC TCAGACCATC ACTGGGCGGA GGTATGGAAG GAGGCATGCA
    TATGTGAACT TTCGGCCTTC TTTAAAGAGC CCAAAAAGAG CATCAAGTCA ACAAATCAAA
    GAGTGCGAAG GACTGGAACT TGATGATGTA CAGACAGAAA AAGATTTAAT TTCCATGGAA
    AGCTGTGATT CACCTCTGTA CGACATTAGT AAATTTTTAA CGACTGACTT GCAACAAGAC
    AACAATAAAC TGAAAGCAAT GGATGATGTT ACATCTTTAG TTACAGAAGT AGAGGAACAT
    GGAGTTTGCC AATCCTTATG TAATAACAAT ATCTGTAAAA AAAACAAGAC TCTTACTAGG
    CATGTACCCC ATAAGTATCA AAATGGAATT TCCTTTTTAA ATATTGACTC TTTTGAGCCA
    GACAGTAGTG AAGGAGAGGA AAACTGTTAC AGTTCAGAGG ATTTTACGTT GACTGAGGAG
    ACCGGCAAAA TTGAAAAATC CTTAGAAAAC ACACTATCTG AGCTTGAGGA GGTGGGGACA
    TTTTGTGAAA TGGAGCCTGA GTTACAAAGT AAAAATCACA GTTGTGCAAA AGACAGATTA
    TCAGAGATGT GGCCTGTATC TTTATGCAAA AGTTCCAAAA TTGATTTTGG GACCACCCAT
    GGCAAATGGA ATGTGTCAGT AAAATCTGTT TCAGAGGATG AGGCCACAGC ACTGGGTATT
    TCTGAATTCG ATTCAAAGAA AAGTAGGACT GATATAACAT TAAAAAATTC TGTTGGACTC
    TATCCTGAAA CTGCTTCTAA TAGCACTACA ATGGAGCAAG ATAGTTTAGC TGAACCAGTT
    GTCAGACCAA AAGTTAGAAA GCAAGGTACA AGTTATGGCA ATAAAAACTC TTCCCTACCG
    GAAGAAAGCA AGGACAATTC AACAGTCCGA AAGAGCAATC GAGGAACACG AGTCAAAGTG
    GAAAATTCCT GCAAAGATGA AAAGAGTTAC CCTGAAATGG AGTCTTCTGG CAGTAATGAT
    GAGGGCACAA GAAAAAAAAT CAAGAGCATT TGCAAAGAGC AAGAATATAC CGACTGCCAT
    GTTGACAAAC CTGCAGTGGA TGATACATTT TGGGATGACT TTGAAATATA TGGAATGAAA
    TTGTCTGGCT CCATTAAGGA CGATGAAAGC TCTGTCTGCA GTGATGGAGA ATGGTCTACA
    TGCTTGCCTT CATATTTTTC TTCGGACAAA GACCATTCGT CAAGTGATGA TAGCTGGGAA
    ACACTGCCTG GAAAAGATGA ACACGAAGTT CTTAGTAACA GCAGTAGTCT GGAAGAAGAA
    AATTCTGACT TTTGTTTTCA AGTAGAGGAA CAAATTTCCT TGGAAGATGG AGAAATCCCT
    TGGTTGCAGT ACCATGAGGA TATTGAAAGC AGCACCGATG AAGAAAATGA AATAAGTAAC
    CAGTTTGTGC ATCCTGGATT TTTCATGTTA GATGGCAATA ACAATTTGGA GGATGATTCA
    AGTATCAGTG AAGACCTGGA AGTTGAATGG AGATTGCTTG ATGATTTTGG TGATGGACTA
    GGAGTTGCAC AAGCCATGTC CTATATGGAC CCTCAGTTTC TTACATATAT GGCTCTTGAG
    GAGCGCCTTG CCCAGGCTAT GGAGACTGCT CTAGCACATT TGGAATCACT TGCCGTTGAT
    GTAGAACAGG CTCACCCACC AGCTACTAAA GAAAGCATAG ACTGCCTCCC TCAGATCATT
    ATTGGTGAAG ACCATAATAT CGTAGGTCAA GAGCAGTGCT GTGCCATCTG CTGCAGTGAG
    TATATCAAAG ATGAAATATT AACTGAACTA CCATGTCACC ACCTTTTCCA TAAGCCCTGT
    GTTACACTTT GGCTACAGAA ATCTGGGACG TGCCCTGTTT GTCGTCACGT GCTTGCATCT
    TCACACACTG ACGCCGCTGC AACATCTTTC CTGTCTGATC ACGAGAGCCC CCCTTCAATC
    CACAGTGCAG CAGGAACCCG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910519.4
    CDS110..2293
    Translation

    Target ORF information:

    RefSeq Version XM_004910462.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis praja ring finger 2, E3 ubiquitin protein ligase (pja2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910462.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGAGCTG CGGGCCTGGG TTACCTGTCC GGAAGGAATC GAGGAGCTAC ATATAAACGG 
    CACTGCGAGA CTTTGAAACA GCGATCCGGG GCTGCGGGCT GTGTAAGGAA AAGCGGGACT
    GTCCCGGCCA AAGCGCCACT ATTGGGCGTG ATGGGTCAGG AAGCATGCAA AGCAGCCTGG
    CCCAAGTCAG CTGGAGGCTC TCAGACCATC ACTGGGCGGA GGTATGGAAG GAGGCATGCA
    TATGTGAACT TTCGGCCTTC TTTAAAGAGC CCAAAAAGAG CATCAAGTCA ACAAATCAAA
    GAGTGCGAAG GACTGGAACT TGATGATGTA CAGACAGAAA AAGATTTAAT TTCCATGGAA
    AGCTGTGATT CACCTCTGTA CGACATTAGT AAATTTTTAA CGACTGACTT GCAACAAGAC
    AACAATAAAC TGAAAGCAAT GGATGATGTT ACATCTTTAG TTACAGAAGT AGAGGAACAT
    GGAGTTTGCC AATCCTTATG TAATAACAAT ATCTGTAAAA AAAACAAGAC TCTTACTAGG
    CATGTACCCC ATAAGTATCA AAATGGAATT TCCTTTTTAA ATATTGACTC TTTTGAGCCA
    GACAGTAGTG AAGGAGAGGA AAACTGTTAC AGTTCAGAGG ATTTTACGTT GACTGAGGAG
    ACCGGCAAAA TTGAAAAATC CTTAGAAAAC ACACTATCTG AGCTTGAGGA GGTGGGGACA
    TTTTGTGAAA TGGAGCCTGA GTTACAAAGT AAAAATCACA GTTGTGCAAA AGACAGATTA
    TCAGAGATGT GGCCTGTATC TTTATGCAAA AGTTCCAAAA TTGATTTTGG GACCACCCAT
    GGCAAATGGA ATGTGTCAGT AAAATCTGTT TCAGAGGATG AGGCCACAGC ACTGGGTATT
    TCTGAATTCG ATTCAAAGAA AAGTAGGACT GATATAACAT TAAAAAATTC TGTTGGACTC
    TATCCTGAAA CTGCTTCTAA TAGCACTACA ATGGAGCAAG ATAGTTTAGC TGAACCAGTT
    GTCAGACCAA AAGTTAGAAA GCAAGGTACA AGTTATGGCA ATAAAAACTC TTCCCTACCG
    GAAGAAAGCA AGGACAATTC AACAGTCCGA AAGAGCAATC GAGGAACACG AGTCAAAGTG
    GAAAATTCCT GCAAAGATGA AAAGAGTTAC CCTGAAATGG AGTCTTCTGG CAGTAATGAT
    GAGGGCACAA GAAAAAAAAT CAAGAGCATT TGCAAAGAGC AAGAATATAC CGACTGCCAT
    GTTGACAAAC CTGCAGTGGA TGATACATTT TGGGATGACT TTGAAATATA TGGAATGAAA
    TTGTCTGGCT CCATTAAGGA CGATGAAAGC TCTGTCTGCA GTGATGGAGA ATGGTCTACA
    TGCTTGCCTT CATATTTTTC TTCGGACAAA GACCATTCGT CAAGTGATGA TAGCTGGGAA
    ACACTGCCTG GAAAAGATGA ACACGAAGTT CTTAGTAACA GCAGTAGTCT GGAAGAAGAA
    AATTCTGACT TTTGTTTTCA AGTAGAGGAA CAAATTTCCT TGGAAGATGG AGAAATCCCT
    TGGTTGCAGT ACCATGAGGA TATTGAAAGC AGCACCGATG AAGAAAATGA AATAAGTAAC
    CAGTTTGTGC ATCCTGGATT TTTCATGTTA GATGGCAATA ACAATTTGGA GGATGATTCA
    AGTATCAGTG AAGACCTGGA AGTTGAATGG AGATTGCTTG ATGATTTTGG TGATGGACTA
    GGAGTTGCAC AAGCCATGTC CTATATGGAC CCTCAGTTTC TTACATATAT GGCTCTTGAG
    GAGCGCCTTG CCCAGGCTAT GGAGACTGCT CTAGCACATT TGGAATCACT TGCCGTTGAT
    GTAGAACAGG CTCACCCACC AGCTACTAAA GAAAGCATAG ACTGCCTCCC TCAGATCATT
    ATTGGTGAAG ACCATAATAT CGTAGGTCAA GAGCAGTGCT GTGCCATCTG CTGCAGTGAG
    TATATCAAAG ATGAAATATT AACTGAACTA CCATGTCACC ACCTTTTCCA TAAGCCCTGT
    GTTACACTTT GGCTACAGAA ATCTGGGACG TGCCCTGTTT GTCGTCACGT GCTTGCATCT
    TCACACACTG ACGCCGCTGC AACATCTTTC CTGTCTGATC ACGAGAGCCC CCCTTCAATC
    CACAGTGCAG CAGGAACCCG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42146
    Clone ID Related Accession (Same CDS sequence) XM_004910462.3
    Accession Version XM_004910462.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2340bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase Praja-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910462.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTTAGTCA CACACTGGGT CCCGGGAGCG CAGGCTGTGG GGATAGCGGA GCCAGCACAG 
    ACTGACAGAC CCGGCCCAAC CCTGCTGCTC CCACTGAGAG GCGAGTGCCC GGAGCGGCGC
    GTCTGTACGG GGCGTGTGAC GTCACACTCT CGCGAGATGA GAGCTGCGGG CCTGGGTTAC
    CTGTCCGGAA GGAATCGAGG AGCTACATAT AAACGGCACT GCGAGACTTT GAAACAGCGA
    TCCGGGGCTG CGGGCTGTGT AAGGAAAAGC GGGACTGTCC CGGCCAAAGC GCCACTATTG
    GGCGTGATGG GTCAGGAAGC ATGCAAAGCA GCCTGGCCCA AGTCAGCTGG AGGCTCTCAG
    ACCATCACTG GGCGGAGGTA TGGAAGGAGG CATGCATATG TGAACTTTCG GCCTTCTTTA
    AAGAGCCCAA AAAGAGCATC AAGTCAACAA ATCAAAGAGT GCGAAGGACT GGAACTTGAT
    GATGTACAGA CAGAAAAAGA TTTAATTTCC ATGGAAAGCT GTGATTCACC TCTGTACGAC
    ATTAGTAAAT TTTTAACGAC TGACTTGCAA CAAGACAACA ATAAACTGAA AGCAATGGAT
    GATGTTACAT CTTTAGTTAC AGAAGTAGAG GAACATGGAG TTTGCCAATC CTTATGTAAT
    AACAATATCT GTAAAAAAAA CAAGACTCTT ACTAGGCATG TACCCCATAA GTATCAAAAT
    GGAATTTCCT TTTTAAATAT TGACTCTTTT GAGCCAGACA GTAGTGAAGG AGAGGAAAAC
    TGTTACAGTT CAGAGGATTT TACGTTGACT GAGGAGACCG GCAAAATTGA AAAATCCTTA
    GAAAACACAC TATCTGAGCT TGAGGAGGTG GGGACATTTT GTGAAATGGA GCCTGAGTTA
    CAAAGTAAAA ATCACAGTTG TGCAAAAGAC AGATTATCAG AGATGTGGCC TGTATCTTTA
    TGCAAAAGTT CCAAAATTGA TTTTGGGACC ACCCATGGCA AATGGAATGT GTCAGTAAAA
    TCTGTTTCAG AGGATGAGGC CACAGCACTG GGTATTTCTG AATTCGATTC AAAGAAAAGT
    AGGACTGATA TAACATTAAA AAATTCTGTT GGACTCTATC CTGAAACTGC TTCTAATAGC
    ACTACAATGG AGCAAGATAG TTTAGCTGAA CCAGTTGTCA GACCAAAAGT TAGAAAGCAA
    GGTACAAGTT ATGGCAATAA AAACTCTTCC CTACCGGAAG AAAGCAAGGA CAATTCAACA
    GTCCGAAAGA GCAATCGAGG AACACGAGTC AAAGTGGAAA ATTCCTGCAA AGATGAAAAG
    AGTTACCCTG AAATGGAGTC TTCTGGCAGT AATGATGAGG GCACAAGAAA AAAAATCAAG
    AGCATTTGCA AAGAGCAAGA ATATACCGAC TGCCATGTTG ACAAACCTGC AGTGGATGAT
    ACATTTTGGG ATGACTTTGA AATATATGGA ATGAAATTGT CTGGCTCCAT TAAGGACGAT
    GAAAGCTCTG TCTGCAGTGA TGGAGAATGG TCTACATGCT TGCCTTCATA TTTTTCTTCG
    GACAAAGACC ATTCGTCAAG TGATGATAGC TGGGAAACAC TGCCTGGAAA AGATGAACAC
    GAAGTTCTTA GTAACAGCAG TAGTCTGGAA GAAGAAAATT CTGACTTTTG TTTTCAAGTA
    GAGGAACAAA TTTCCTTGGA AGATGGAGAA ATCCCTTGGT TGCAGTACCA TGAGGATATT
    GAAAGCAGCA CCGATGAAGA AAATGAAATA AGTAACCAGT TTGTGCATCC TGGATTTTTC
    ATGTTAGATG GCAATAACAA TTTGGAGGAT GATTCAAGTA TCAGTGAAGA CCTGGAAGTT
    GAATGGAGAT TGCTTGATGA TTTTGGTGAT GGACTAGGAG TTGCACAAGC CATGTCCTAT
    ATGGACCCTC AGTTTCTTAC ATATATGGCT CTTGAGGAGC GCCTTGCCCA GGCTATGGAG
    ACTGCTCTAG CACATTTGGA ATCACTTGCC GTTGATGTAG AACAGGCTCA CCCACCAGCT
    ACTAAAGAAA GCATAGACTG CCTCCCTCAG ATCATTATTG GTGAAGACCA TAATATCGTA
    GGTCAAGAGC AGTGCTGTGC CATCTGCTGC AGTGAGTATA TCAAAGATGA AATATTAACT
    GAACTACCAT GTCACCACCT TTTCCATAAG CCCTGTGTTA CACTTTGGCT ACAGAAATCT
    GGGACGTGCC CTGTTTGTCG TCACGTGCTT GCATCTTCAC ACACTGACGC CGCTGCAACA
    TCTTTCCTGT CTGATCACGA GAGCCCCCCT TCAATCCACA GTGCAGCAGG AACCCGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910519.3
    CDS90..2429
    Translation

    Target ORF information:

    RefSeq Version XM_004910462.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis praja ring finger 2, E3 ubiquitin protein ligase (pja2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910462.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTTAGTCA CACACTGGGT CCCGGGAGCG CAGGCTGTGG GGATAGCGGA GCCAGCACAG 
    ACTGACAGAC CCGGCCCAAC CCTGCTGCTC CCACTGAGAG GCGAGTGCCC GGAGCGGCGC
    GTCTGTACGG GGCGTGTGAC GTCACACTCT CGCGAGATGA GAGCTGCGGG CCTGGGTTAC
    CTGTCCGGAA GGAATCGAGG AGCTACATAT AAACGGCACT GCGAGACTTT GAAACAGCGA
    TCCGGGGCTG CGGGCTGTGT AAGGAAAAGC GGGACTGTCC CGGCCAAAGC GCCACTATTG
    GGCGTGATGG GTCAGGAAGC ATGCAAAGCA GCCTGGCCCA AGTCAGCTGG AGGCTCTCAG
    ACCATCACTG GGCGGAGGTA TGGAAGGAGG CATGCATATG TGAACTTTCG GCCTTCTTTA
    AAGAGCCCAA AAAGAGCATC AAGTCAACAA ATCAAAGAGT GCGAAGGACT GGAACTTGAT
    GATGTACAGA CAGAAAAAGA TTTAATTTCC ATGGAAAGCT GTGATTCACC TCTGTACGAC
    ATTAGTAAAT TTTTAACGAC TGACTTGCAA CAAGACAACA ATAAACTGAA AGCAATGGAT
    GATGTTACAT CTTTAGTTAC AGAAGTAGAG GAACATGGAG TTTGCCAATC CTTATGTAAT
    AACAATATCT GTAAAAAAAA CAAGACTCTT ACTAGGCATG TACCCCATAA GTATCAAAAT
    GGAATTTCCT TTTTAAATAT TGACTCTTTT GAGCCAGACA GTAGTGAAGG AGAGGAAAAC
    TGTTACAGTT CAGAGGATTT TACGTTGACT GAGGAGACCG GCAAAATTGA AAAATCCTTA
    GAAAACACAC TATCTGAGCT TGAGGAGGTG GGGACATTTT GTGAAATGGA GCCTGAGTTA
    CAAAGTAAAA ATCACAGTTG TGCAAAAGAC AGATTATCAG AGATGTGGCC TGTATCTTTA
    TGCAAAAGTT CCAAAATTGA TTTTGGGACC ACCCATGGCA AATGGAATGT GTCAGTAAAA
    TCTGTTTCAG AGGATGAGGC CACAGCACTG GGTATTTCTG AATTCGATTC AAAGAAAAGT
    AGGACTGATA TAACATTAAA AAATTCTGTT GGACTCTATC CTGAAACTGC TTCTAATAGC
    ACTACAATGG AGCAAGATAG TTTAGCTGAA CCAGTTGTCA GACCAAAAGT TAGAAAGCAA
    GGTACAAGTT ATGGCAATAA AAACTCTTCC CTACCGGAAG AAAGCAAGGA CAATTCAACA
    GTCCGAAAGA GCAATCGAGG AACACGAGTC AAAGTGGAAA ATTCCTGCAA AGATGAAAAG
    AGTTACCCTG AAATGGAGTC TTCTGGCAGT AATGATGAGG GCACAAGAAA AAAAATCAAG
    AGCATTTGCA AAGAGCAAGA ATATACCGAC TGCCATGTTG ACAAACCTGC AGTGGATGAT
    ACATTTTGGG ATGACTTTGA AATATATGGA ATGAAATTGT CTGGCTCCAT TAAGGACGAT
    GAAAGCTCTG TCTGCAGTGA TGGAGAATGG TCTACATGCT TGCCTTCATA TTTTTCTTCG
    GACAAAGACC ATTCGTCAAG TGATGATAGC TGGGAAACAC TGCCTGGAAA AGATGAACAC
    GAAGTTCTTA GTAACAGCAG TAGTCTGGAA GAAGAAAATT CTGACTTTTG TTTTCAAGTA
    GAGGAACAAA TTTCCTTGGA AGATGGAGAA ATCCCTTGGT TGCAGTACCA TGAGGATATT
    GAAAGCAGCA CCGATGAAGA AAATGAAATA AGTAACCAGT TTGTGCATCC TGGATTTTTC
    ATGTTAGATG GCAATAACAA TTTGGAGGAT GATTCAAGTA TCAGTGAAGA CCTGGAAGTT
    GAATGGAGAT TGCTTGATGA TTTTGGTGAT GGACTAGGAG TTGCACAAGC CATGTCCTAT
    ATGGACCCTC AGTTTCTTAC ATATATGGCT CTTGAGGAGC GCCTTGCCCA GGCTATGGAG
    ACTGCTCTAG CACATTTGGA ATCACTTGCC GTTGATGTAG AACAGGCTCA CCCACCAGCT
    ACTAAAGAAA GCATAGACTG CCTCCCTCAG ATCATTATTG GTGAAGACCA TAATATCGTA
    GGTCAAGAGC AGTGCTGTGC CATCTGCTGC AGTGAGTATA TCAAAGATGA AATATTAACT
    GAACTACCAT GTCACCACCT TTTCCATAAG CCCTGTGTTA CACTTTGGCT ACAGAAATCT
    GGGACGTGCC CTGTTTGTCG TCACGTGCTT GCATCTTCAC ACACTGACGC CGCTGCAACA
    TCTTTCCTGT CTGATCACGA GAGCCCCCCT TCAATCCACA GTGCAGCAGG AACCCGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42147
    Clone ID Related Accession (Same CDS sequence) XM_018089900.1
    Accession Version XM_018089900.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase Praja-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTTAGTCA CACACTGGGT CCCGGGAGCG CAGGCTGTGG GGATAGCGGA GCCAGCACAG 
    ACTGACAGAC CCGGCCCAAC CCTGCTGCTC CCACTGAGAG GCGAGTGCCC GGAGCGGCGC
    GTCTGTACGG GGCGTGTGAC GTCACACTCT CGCGAGATGA GAGCTGCGGG CCTGGGTTAC
    CTGTCCGGAA GGAATCGAGG AGCTACATAT AAACGGCACT GCGAGACTTT GAAACAGCGA
    TCCGGGGCTG CGGGCTGTGT AAGGAAAAGC GGGACTGTCC CGGCCAAAGC GCCACTATTG
    GGCGTGATGG GTCAGGAAGC ATGCAAAGCA GCCTGGCCCA AGTCAGCTGG AGGCTCTCAG
    ACCATCACTG GGCGGAGGTA TGGAAGGAGG CATGCATATG TGAACTTTCG GCCTTCTTTA
    AAGAGCCCAA AAAGAGCATC AAGTCAACAA ATCAAAGAGT GCGAAGGACT GGAACTTGAT
    GATGTACAGA CAGAAAAAGA TTTAATTTCC ATGGAAAGCT GTGATTCACC TCTGTACGAC
    ATTAGTAAAT TTTTAACGAC TGACTTGCAA CAAGACAACA ATAAACTGAA AGCAATGGAT
    GATGTTACAT CTTTAGTTAC AGAAGTAGAG GAACATGGAG TTTGCCAATC CTTATGTAAT
    AACAATATCT GTAAAAAAAA CAAGACTCTT ACTAGGCATG TACCCCATAA GTATCAAAAT
    GGAATTTCCT TTTTAAATAT TGACTCTTTT GAGCCAGACA GTAGTGAAGG AGAGGAAAAC
    TGTTACAGTT CAGAGGATTT TACGTTGACT GAGGAGACCG GCAAAATTGA AAAATCCTTA
    GAAAACACAC TATCTGAGCT TGAGGAGGTG GGGACATTTT GTGAAATGGA GCCTGAGTTA
    CAAAGTAAAA ATCACAGTTG TGCAAAAGAC AGATTATCAG AGATGTGGCC TGTATCTTTA
    TGCAAAAGTT CCAAAATTGA TTTTGGGACC ACCCATGGCA AATGGAATGT GTCAGTAAAA
    TCTGTTTCAG AGGATGAGGC CACAGCACTG GGTATTTCTG AATTCGATTC AAAGAAAAGT
    AGGACTGATA TAACATTAAA AAATTCTGTT GGACTCTATC CTGAAACTGC TTCTAATAGC
    ACTACAATGG AGCAAGATAG TTTAGCTGAA CCAGTTGTCA GACCAAAAGT TAGAAAGCAA
    GGTACAAGTT ATGGCAATAA AAACTCTTCC CTACCGGAAG AAAGCAAGGA CAATTCAACA
    GTCCGAAAGA GCAATCGAGG AACACGAGTC AAAGTGGAAA ATTCCTGCAA AGATGAAAAG
    AGTTACCCTG AAATGGAGTC TTCTGGCAGT AATGATGAGG GCACAAGAAA AAAAATCAAG
    AGCATTTGCA AAGAGCAAGA ATATACCGAC TGCCATGTTG ACAAACCTGC AGTGGATGAT
    ACATTTTGGG ATGACTTTGA AATATATGGA ATGAAATTGT CTGGCTCCAT TAAGGACGAT
    GAAAGTGATG GAGAATGGTC TACATGCTTG CCTTCATATT TTTCTTCGGA CAAAGACCAT
    TCGTCAAGTG ATGATAGCTG GGAAACACTG CCTGGAAAAG ATGAACACGA AGTTCTTAGT
    AACAGCAGTA GTCTGGAAGA AGAAAATTCT GACTTTTGTT TTCAAGTAGA GGAACAAATT
    TCCTTGGAAG ATGGAGAAAT CCCTTGGTTG CAGTACCATG AGGATATTGA AAGCAGCACC
    GATGAAGAAA ATGAAATAAG TAACCAGTTT GTGCATCCTG GATTTTTCAT GTTAGATGGC
    AATAACAATT TGGAGGATGA TTCAAGTATC AGTGAAGACC TGGAAGTTGA ATGGAGATTG
    CTTGATGATT TTGGTGATGG ACTAGGAGTT GCACAAGCCA TGTCCTATAT GGACCCTCAG
    TTTCTTACAT ATATGGCTCT TGAGGAGCGC CTTGCCCAGG CTATGGAGAC TGCTCTAGCA
    CATTTGGAAT CACTTGCCGT TGATGTAGAA CAGGCTCACC CACCAGCTAC TAAAGAAAGC
    ATAGACTGCC TCCCTCAGAT CATTATTGGT GAAGACCATA ATATCGTAGG TCAAGAGCAG
    TGCTGTGCCA TCTGCTGCAG TGAGTATATC AAAGATGAAA TATTAACTGA ACTACCATGT
    CACCACCTTT TCCATAAGCC CTGTGTTACA CTTTGGCTAC AGAAATCTGG GACGTGCCCT
    GTTTGTCGTC ACGTGCTTGC ATCTTCACAC ACTGACGCCG CTGCAACATC TTTCCTGTCT
    GATCACGAGA GCCCCCCTTC AATCCACAGT GCAGCAGGAA CCCGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017945389.1
    CDS96..2423
    Translation

    Target ORF information:

    RefSeq Version XM_018089900.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis praja ring finger 2, E3 ubiquitin protein ligase (pja2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089900.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGTTAGTCA CACACTGGGT CCCGGGAGCG CAGGCTGTGG GGATAGCGGA GCCAGCACAG 
    ACTGACAGAC CCGGCCCAAC CCTGCTGCTC CCACTGAGAG GCGAGTGCCC GGAGCGGCGC
    GTCTGTACGG GGCGTGTGAC GTCACACTCT CGCGAGATGA GAGCTGCGGG CCTGGGTTAC
    CTGTCCGGAA GGAATCGAGG AGCTACATAT AAACGGCACT GCGAGACTTT GAAACAGCGA
    TCCGGGGCTG CGGGCTGTGT AAGGAAAAGC GGGACTGTCC CGGCCAAAGC GCCACTATTG
    GGCGTGATGG GTCAGGAAGC ATGCAAAGCA GCCTGGCCCA AGTCAGCTGG AGGCTCTCAG
    ACCATCACTG GGCGGAGGTA TGGAAGGAGG CATGCATATG TGAACTTTCG GCCTTCTTTA
    AAGAGCCCAA AAAGAGCATC AAGTCAACAA ATCAAAGAGT GCGAAGGACT GGAACTTGAT
    GATGTACAGA CAGAAAAAGA TTTAATTTCC ATGGAAAGCT GTGATTCACC TCTGTACGAC
    ATTAGTAAAT TTTTAACGAC TGACTTGCAA CAAGACAACA ATAAACTGAA AGCAATGGAT
    GATGTTACAT CTTTAGTTAC AGAAGTAGAG GAACATGGAG TTTGCCAATC CTTATGTAAT
    AACAATATCT GTAAAAAAAA CAAGACTCTT ACTAGGCATG TACCCCATAA GTATCAAAAT
    GGAATTTCCT TTTTAAATAT TGACTCTTTT GAGCCAGACA GTAGTGAAGG AGAGGAAAAC
    TGTTACAGTT CAGAGGATTT TACGTTGACT GAGGAGACCG GCAAAATTGA AAAATCCTTA
    GAAAACACAC TATCTGAGCT TGAGGAGGTG GGGACATTTT GTGAAATGGA GCCTGAGTTA
    CAAAGTAAAA ATCACAGTTG TGCAAAAGAC AGATTATCAG AGATGTGGCC TGTATCTTTA
    TGCAAAAGTT CCAAAATTGA TTTTGGGACC ACCCATGGCA AATGGAATGT GTCAGTAAAA
    TCTGTTTCAG AGGATGAGGC CACAGCACTG GGTATTTCTG AATTCGATTC AAAGAAAAGT
    AGGACTGATA TAACATTAAA AAATTCTGTT GGACTCTATC CTGAAACTGC TTCTAATAGC
    ACTACAATGG AGCAAGATAG TTTAGCTGAA CCAGTTGTCA GACCAAAAGT TAGAAAGCAA
    GGTACAAGTT ATGGCAATAA AAACTCTTCC CTACCGGAAG AAAGCAAGGA CAATTCAACA
    GTCCGAAAGA GCAATCGAGG AACACGAGTC AAAGTGGAAA ATTCCTGCAA AGATGAAAAG
    AGTTACCCTG AAATGGAGTC TTCTGGCAGT AATGATGAGG GCACAAGAAA AAAAATCAAG
    AGCATTTGCA AAGAGCAAGA ATATACCGAC TGCCATGTTG ACAAACCTGC AGTGGATGAT
    ACATTTTGGG ATGACTTTGA AATATATGGA ATGAAATTGT CTGGCTCCAT TAAGGACGAT
    GAAAGTGATG GAGAATGGTC TACATGCTTG CCTTCATATT TTTCTTCGGA CAAAGACCAT
    TCGTCAAGTG ATGATAGCTG GGAAACACTG CCTGGAAAAG ATGAACACGA AGTTCTTAGT
    AACAGCAGTA GTCTGGAAGA AGAAAATTCT GACTTTTGTT TTCAAGTAGA GGAACAAATT
    TCCTTGGAAG ATGGAGAAAT CCCTTGGTTG CAGTACCATG AGGATATTGA AAGCAGCACC
    GATGAAGAAA ATGAAATAAG TAACCAGTTT GTGCATCCTG GATTTTTCAT GTTAGATGGC
    AATAACAATT TGGAGGATGA TTCAAGTATC AGTGAAGACC TGGAAGTTGA ATGGAGATTG
    CTTGATGATT TTGGTGATGG ACTAGGAGTT GCACAAGCCA TGTCCTATAT GGACCCTCAG
    TTTCTTACAT ATATGGCTCT TGAGGAGCGC CTTGCCCAGG CTATGGAGAC TGCTCTAGCA
    CATTTGGAAT CACTTGCCGT TGATGTAGAA CAGGCTCACC CACCAGCTAC TAAAGAAAGC
    ATAGACTGCC TCCCTCAGAT CATTATTGGT GAAGACCATA ATATCGTAGG TCAAGAGCAG
    TGCTGTGCCA TCTGCTGCAG TGAGTATATC AAAGATGAAA TATTAACTGA ACTACCATGT
    CACCACCTTT TCCATAAGCC CTGTGTTACA CTTTGGCTAC AGAAATCTGG GACGTGCCCT
    GTTTGTCGTC ACGTGCTTGC ATCTTCACAC ACTGACGCCG CTGCAACATC TTTCCTGTCT
    GATCACGAGA GCCCCCCTTC AATCCACAGT GCAGCAGGAA CCCGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49062
    Clone ID Related Accession (Same CDS sequence) XM_018089900.2
    Accession Version XM_018089900.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2172bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase Praja-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018089900.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGAGCTG CGGGCCTGGG TTACCTGTCC GGAAGGAATC GAGGAGCTAC ATATAAACGG 
    CACTGCGAGA CTTTGAAACA GCGATCCGGG GCTGCGGGCT GTGTAAGGAA AAGCGGGACT
    GTCCCGGCCA AAGCGCCACT ATTGGGCGTG ATGGGTCAGG AAGCATGCAA AGCAGCCTGG
    CCCAAGTCAG CTGGAGGCTC TCAGACCATC ACTGGGCGGA GGTATGGAAG GAGGCATGCA
    TATGTGAACT TTCGGCCTTC TTTAAAGAGC CCAAAAAGAG CATCAAGTCA ACAAATCAAA
    GAGTGCGAAG GACTGGAACT TGATGATGTA CAGACAGAAA AAGATTTAAT TTCCATGGAA
    AGCTGTGATT CACCTCTGTA CGACATTAGT AAATTTTTAA CGACTGACTT GCAACAAGAC
    AACAATAAAC TGAAAGCAAT GGATGATGTT ACATCTTTAG TTACAGAAGT AGAGGAACAT
    GGAGTTTGCC AATCCTTATG TAATAACAAT ATCTGTAAAA AAAACAAGAC TCTTACTAGG
    CATGTACCCC ATAAGTATCA AAATGGAATT TCCTTTTTAA ATATTGACTC TTTTGAGCCA
    GACAGTAGTG AAGGAGAGGA AAACTGTTAC AGTTCAGAGG ATTTTACGTT GACTGAGGAG
    ACCGGCAAAA TTGAAAAATC CTTAGAAAAC ACACTATCTG AGCTTGAGGA GGTGGGGACA
    TTTTGTGAAA TGGAGCCTGA GTTACAAAGT AAAAATCACA GTTGTGCAAA AGACAGATTA
    TCAGAGATGT GGCCTGTATC TTTATGCAAA AGTTCCAAAA TTGATTTTGG GACCACCCAT
    GGCAAATGGA ATGTGTCAGT AAAATCTGTT TCAGAGGATG AGGCCACAGC ACTGGGTATT
    TCTGAATTCG ATTCAAAGAA AAGTAGGACT GATATAACAT TAAAAAATTC TGTTGGACTC
    TATCCTGAAA CTGCTTCTAA TAGCACTACA ATGGAGCAAG ATAGTTTAGC TGAACCAGTT
    GTCAGACCAA AAGTTAGAAA GCAAGGTACA AGTTATGGCA ATAAAAACTC TTCCCTACCG
    GAAGAAAGCA AGGACAATTC AACAGTCCGA AAGAGCAATC GAGGAACACG AGTCAAAGTG
    GAAAATTCCT GCAAAGATGA AAAGAGTTAC CCTGAAATGG AGTCTTCTGG CAGTAATGAT
    GAGGGCACAA GAAAAAAAAT CAAGAGCATT TGCAAAGAGC AAGAATATAC CGACTGCCAT
    GTTGACAAAC CTGCAGTGGA TGATACATTT TGGGATGACT TTGAAATATA TGGAATGAAA
    TTGTCTGGCT CCATTAAGGA CGATGAAAGT GATGGAGAAT GGTCTACATG CTTGCCTTCA
    TATTTTTCTT CGGACAAAGA CCATTCGTCA AGTGATGATA GCTGGGAAAC ACTGCCTGGA
    AAAGATGAAC ACGAAGTTCT TAGTAACAGC AGTAGTCTGG AAGAAGAAAA TTCTGACTTT
    TGTTTTCAAG TAGAGGAACA AATTTCCTTG GAAGATGGAG AAATCCCTTG GTTGCAGTAC
    CATGAGGATA TTGAAAGCAG CACCGATGAA GAAAATGAAA TAAGTAACCA GTTTGTGCAT
    CCTGGATTTT TCATGTTAGA TGGCAATAAC AATTTGGAGG ATGATTCAAG TATCAGTGAA
    GACCTGGAAG TTGAATGGAG ATTGCTTGAT GATTTTGGTG ATGGACTAGG AGTTGCACAA
    GCCATGTCCT ATATGGACCC TCAGTTTCTT ACATATATGG CTCTTGAGGA GCGCCTTGCC
    CAGGCTATGG AGACTGCTCT AGCACATTTG GAATCACTTG CCGTTGATGT AGAACAGGCT
    CACCCACCAG CTACTAAAGA AAGCATAGAC TGCCTCCCTC AGATCATTAT TGGTGAAGAC
    CATAATATCG TAGGTCAAGA GCAGTGCTGT GCCATCTGCT GCAGTGAGTA TATCAAAGAT
    GAAATATTAA CTGAACTACC ATGTCACCAC CTTTTCCATA AGCCCTGTGT TACACTTTGG
    CTACAGAAAT CTGGGACGTG CCCTGTTTGT CGTCACGTGC TTGCATCTTC ACACACTGAC
    GCCGCTGCAA CATCTTTCCT GTCTGATCAC GAGAGCCCCC CTTCAATCCA CAGTGCAGCA
    GGAACCCGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017945389.2
    CDS113..2284
    Translation

    Target ORF information:

    RefSeq Version XM_018089900.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis praja ring finger 2, E3 ubiquitin protein ligase (pja2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018089900.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAGAGCTG CGGGCCTGGG TTACCTGTCC GGAAGGAATC GAGGAGCTAC ATATAAACGG 
    CACTGCGAGA CTTTGAAACA GCGATCCGGG GCTGCGGGCT GTGTAAGGAA AAGCGGGACT
    GTCCCGGCCA AAGCGCCACT ATTGGGCGTG ATGGGTCAGG AAGCATGCAA AGCAGCCTGG
    CCCAAGTCAG CTGGAGGCTC TCAGACCATC ACTGGGCGGA GGTATGGAAG GAGGCATGCA
    TATGTGAACT TTCGGCCTTC TTTAAAGAGC CCAAAAAGAG CATCAAGTCA ACAAATCAAA
    GAGTGCGAAG GACTGGAACT TGATGATGTA CAGACAGAAA AAGATTTAAT TTCCATGGAA
    AGCTGTGATT CACCTCTGTA CGACATTAGT AAATTTTTAA CGACTGACTT GCAACAAGAC
    AACAATAAAC TGAAAGCAAT GGATGATGTT ACATCTTTAG TTACAGAAGT AGAGGAACAT
    GGAGTTTGCC AATCCTTATG TAATAACAAT ATCTGTAAAA AAAACAAGAC TCTTACTAGG
    CATGTACCCC ATAAGTATCA AAATGGAATT TCCTTTTTAA ATATTGACTC TTTTGAGCCA
    GACAGTAGTG AAGGAGAGGA AAACTGTTAC AGTTCAGAGG ATTTTACGTT GACTGAGGAG
    ACCGGCAAAA TTGAAAAATC CTTAGAAAAC ACACTATCTG AGCTTGAGGA GGTGGGGACA
    TTTTGTGAAA TGGAGCCTGA GTTACAAAGT AAAAATCACA GTTGTGCAAA AGACAGATTA
    TCAGAGATGT GGCCTGTATC TTTATGCAAA AGTTCCAAAA TTGATTTTGG GACCACCCAT
    GGCAAATGGA ATGTGTCAGT AAAATCTGTT TCAGAGGATG AGGCCACAGC ACTGGGTATT
    TCTGAATTCG ATTCAAAGAA AAGTAGGACT GATATAACAT TAAAAAATTC TGTTGGACTC
    TATCCTGAAA CTGCTTCTAA TAGCACTACA ATGGAGCAAG ATAGTTTAGC TGAACCAGTT
    GTCAGACCAA AAGTTAGAAA GCAAGGTACA AGTTATGGCA ATAAAAACTC TTCCCTACCG
    GAAGAAAGCA AGGACAATTC AACAGTCCGA AAGAGCAATC GAGGAACACG AGTCAAAGTG
    GAAAATTCCT GCAAAGATGA AAAGAGTTAC CCTGAAATGG AGTCTTCTGG CAGTAATGAT
    GAGGGCACAA GAAAAAAAAT CAAGAGCATT TGCAAAGAGC AAGAATATAC CGACTGCCAT
    GTTGACAAAC CTGCAGTGGA TGATACATTT TGGGATGACT TTGAAATATA TGGAATGAAA
    TTGTCTGGCT CCATTAAGGA CGATGAAAGT GATGGAGAAT GGTCTACATG CTTGCCTTCA
    TATTTTTCTT CGGACAAAGA CCATTCGTCA AGTGATGATA GCTGGGAAAC ACTGCCTGGA
    AAAGATGAAC ACGAAGTTCT TAGTAACAGC AGTAGTCTGG AAGAAGAAAA TTCTGACTTT
    TGTTTTCAAG TAGAGGAACA AATTTCCTTG GAAGATGGAG AAATCCCTTG GTTGCAGTAC
    CATGAGGATA TTGAAAGCAG CACCGATGAA GAAAATGAAA TAAGTAACCA GTTTGTGCAT
    CCTGGATTTT TCATGTTAGA TGGCAATAAC AATTTGGAGG ATGATTCAAG TATCAGTGAA
    GACCTGGAAG TTGAATGGAG ATTGCTTGAT GATTTTGGTG ATGGACTAGG AGTTGCACAA
    GCCATGTCCT ATATGGACCC TCAGTTTCTT ACATATATGG CTCTTGAGGA GCGCCTTGCC
    CAGGCTATGG AGACTGCTCT AGCACATTTG GAATCACTTG CCGTTGATGT AGAACAGGCT
    CACCCACCAG CTACTAAAGA AAGCATAGAC TGCCTCCCTC AGATCATTAT TGGTGAAGAC
    CATAATATCG TAGGTCAAGA GCAGTGCTGT GCCATCTGCT GCAGTGAGTA TATCAAAGAT
    GAAATATTAA CTGAACTACC ATGTCACCAC CTTTTCCATA AGCCCTGTGT TACACTTTGG
    CTACAGAAAT CTGGGACGTG CCCTGTTTGT CGTCACGTGC TTGCATCTTC ACACACTGAC
    GCCGCTGCAA CATCTTTCCT GTCTGATCAC GAGAGCCCCC CTTCAATCCA CAGTGCAGCA
    GGAACCCGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.