Prpf8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prpf8
Entrez Gene ID	192159
Full Name	pre-mRNA processing factor 8
Synonyms	AU019467,D11Bwg0410e,DBF3/PRP8,Prp8,Sfprp8l
General protein information	Preferred Names pre-mRNA processing factor 8 Names pre-mRNA-processing-splicing factor 8 splicing factor Prp8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B5\|11 45.92 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_138659.2	NP_619600.2	pre-mRNA-processing-splicing factor 8

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prpf8	NP_001178519.1
Neurospora crassa	NCU07832	XP_962953.1
Arabidopsis thaliana (thale cress)	SUS2	NP_178124.2
	PRPF8	XP_001117328.1
Canis lupus familiaris (dog)	PRPF8	XP_854384.1
Drosophila melanogaster (fruit fly)	Prp8	NP_610735.1
Caenorhabditis elegans (roundworm)	prp-8	NP_498785.1
Schizosaccharomyces pombe (fission yeast)	spp42	NP_593861.1
Magnaporthe oryzae (rice blast fungus)	MGG_05244	XP_003712798.1
Oryza sativa (rice)	Os06g0167000	NP_001056917.1
Homo sapiens (human)	PRPF8	NP_006436.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006885	XP_308873.2
Kluyveromyces lactis	KLLA0A05280g	XP_451233.1
Eremothecium gossypii	AGOS_ABR207W	NP_983156.2
Bos taurus (cattle)	PRPF8	NP_001099108.1
Mus musculus (house mouse)	Prpf8	NP_619600.2
Arabidopsis thaliana (thale cress)	AT4G38780	NP_195589.2
Xenopus tropicalis (tropical clawed frog)	prpf8	XP_002936105.1
Gallus gallus (chicken)	PRPF8	XP_415805.2
Danio rerio (zebrafish)	prpf8	NP_957270.2
Saccharomyces cerevisiae (baker's yeast)	PRP8	NP_012035.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in pre-mRNA processing factors 3, 8, and 31 cause dysfunction of the retinal pigment epithelium.
Farkas MH, Lew DS, Sousa ME, Bujakowska K, Chatagnon J, Bhattacharya SS, Pierce EA, Nandrot EF
The American journal of pathology184(10)2641-52(2014 Oct)

Erbb2 is required for cardiac atrial electrical activity during development.
Tenin G, Clowes C, Wolton K, Krejci E, Wright JA, Lovell SC, Sedmera D, Hentges KE
PloS one9(9)e107041(2014)

Three gene-targeted mouse models of RNA splicing factor RP show late-onset RPE and retinal degeneration.
Graziotto JJ, Farkas MH, Bujakowska K, Deramaudt BM, Zhang Q, Nandrot EF, Inglehearn CF, Bhattacharya SS, Pierce EA
Investigative ophthalmology & visual science52(1)190-8(2011 Jan)

Temporal and tissue specific regulation of RP-associated splicing factor genes PRPF3, PRPF31 and PRPC8--implications in the pathogenesis of RP.
Cao H, Wu J, Lam S, Duan R, Newnham C, Molday RS, Graziotto JJ, Pierce EA, Hu J
PloS one6(1)e15860(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The mouse retinal pigment epithelium (RPE)is the primary cell affected by mutations in the RNA splicing factors (PRPF3, PRPF8, and PRPF31), and these changes occur at an early age.
Title: Mutations in pre-mRNA processing factors 3, 8, and 31 cause dysfunction of the retinal pigment epithelium.

The finding of similar degenerative changes in RPE cells of all three mouse models suggests that the retinal pigment epithelium may be the primary cell type affected in the RNA splicing factor forms of retinitis pigmentosa.
Title: Three gene-targeted mouse models of RNA splicing factor RP show late-onset RPE and retinal degeneration.

found that PRP31 and PRPC8 as well as snRNAs are highly expressed in retinal cells
Title: Temporal and tissue specific regulation of RP-associated splicing factor genes PRPF3, PRPF31 and PRPC8--implications in the pathogenesis of RP.

The following Prpf8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prpf8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10831	NM_138659.2 Latest version!	Mus musculus pre-mRNA processing factor 8 (Prpf8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1714.30 $2449.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prpf8 ( NM_138659.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_619600.2 Mus musculus pre-mRNA processing factor 8 (Prpf8), mRNA.

Price & Availability↑

CloneID	OMu10831
Clone ID Related Accession (Same CDS sequence)	NM_138659.2
Accession Version	NM_138659.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ120731.1, AK041017.1, AK144232.1, CK636594.1, CN530184.1, CB558040.1, BU510116.1, CO426130.1, CV561799.1 and BP757620.1. On Oct 6, 2006 this sequence version replaced NM_138659.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC093481.1, AB047391.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGAG TGTTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCCGGCCC TCTAGCCCCG 
CTGCCGGATT ATATGTCAGA GGAGAAGCTG CAGGAGAAAG CTCGAAAATG GCAGCAGTTG 
CAGGCCAAGC GATATGCAGA GAAGCGGAAA TTTGGGTTTG TGGATGCTCA GAAGGAAGAC 
ATGCCACCAG AACATGTCAG GAAGATCATT CGAGACCATG GAGACATGAC CAACAGGAAG 
TTCCGTCATG ACAAAAGGGT CTATTTGGGA GCTCTTAAGT ACATGCCTCA TGCAGTCCTC 
AAGCTCCTTG AGAACATGCC CATGCCTTGG GAGCAGATCC GGGACGTGCC TGTGCTGTAC 
CACATCACTG GAGCCATCTC CTTTGTTAAC GAGATTCCCT GGGTTATCGA GCCGGTTTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATCATG ATGCGTAGAG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTCGAT GACGAGGAAC CGCCCCTGGA TTATGCCGAC 
AACATCTTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC AGAGGAGGAT 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCTTTGA GAGACAGTAG GAAGTACGTT 
AATGGTTCCA CCTACCAACG CTGGCAGTTC ACATTGCCTA TGATGTCCAC ACTCTACCGA 
CTGGCCAATC AGCTTCTGAC TGACCTTGTA GATGACAACT ACTTCTATCT CTTTGATCTG 
AAGGCCTTTT TTACCTCTAA GGCACTCAAC ATGGCCATTC CTGGGGGACC CAAATTTGAG 
CCTCTTGTTC GAGACATCAA CTTACAGGAT GAAGACTGGA ATGAATTTAA CGATATTAAT 
AAGATCATCA TCCGTCAGCC TATTCGTACT GAGTACAAGA TTGCTTTTCC TTACCTGTAT 
AACAACCTTC CACACCACGT CCACCTGACC TGGTACCACA CTCCCAACGT TGTGTTCATC 
AAGACTGAGG ATCCTGATCT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATCTCC 
CATAGACACT CAGTCAAGAG CCAAGAACCA CTGCCTGATG ATGATGAAGA GTTTGAACTC 
CCAGAGTTTG TCGAGCCCTT CCTGAAGGAT ACACCTCTCT ATACAGACAA CACAGCCAAT 
GGCATTGCCC TGCTCTGGGC ACCACGGCCC TTCAACCTAC GCTCTGGTCG TACCCGCCGG 
GCCTTGGACA TACCCCTTGT CAAGAACTGG TATCGGGAGC ACTGTCCTGC TGGGCAGCCT 
GTGAAGGTTC GAGTCTCTTA TCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTCAAG 
CACCGGCCTC CCAAAGCCCA GAAGAAGAGG TATCTGTTCC GTTCTTTCAA AGCTACCAAA 
TTCTTTCAGT CCACCAAGCT GGACTGGGTG GAGGTAGGGC TGCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGG AAGAACCTCA ACTATCTGCA CTTGGACTAC 
AACTTCAACC TGAAGCCTGT CAAGACGCTC ACCACCAAGG AAAGAAAGAA ATCCCGTTTT 
GGGAATGCTT TCCATCTATG TCGGGAAGTA CTGCGTTTAA CCAAGCTGGT TGTGGACAGC 
CATGTGCAGT ATCGCTTGGG CAATGTAGAT GCCTTCCAGC TGGCAGATGG TTTGCAGTAT 
ATATTTGCCC ACGTGGGACA GTTGACAGGC ATGTATCGAT ACAAATATAA GCTGATGCGG 
CAGATCCGCA TGTGCAAGGA CTTGAAACAT CTCATCTATT ACCGCTTCAA CACGGGTCCT 
GTAGGGAAAG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTCTTT 
ATGCGTGGAA TTACCCCTCT GCTAGAGCGA TGGCTGGGCA ACCTTCTGGC CCGACAGTTT 
GAAGGCCGTC ACTCAAAGGG GGTGGCAAAG ACAGTAACGA AGCAGCGGGT GGAGTCGCAC 
TTTGACCTGG AGCTCAGGGC TGCTGTGATG CACGACATTC TGGACATGAT GCCTGAAGGC 
ATCAAGCAGA ACAAGGCCCG GACAATTCTG CAGCACCTCA GTGAAGCCTG GCGCTGCTGG 
AAGGCCAACA TCCCTTGGAA GGTCCCAGGA CTGCCAACAC CTATAGAGAA TATGATTCTT 
CGGTACGTTA AGGCCAAGGC TGATTGGTGG ACCAATACTG CCCACTACAA CCGGGAACGG 
ATCCGTCGCG GGGCTACTGT AGACAAGACT GTTTGCAAAA AGAACCTGGG CCGCCTCACT 
CGCCTGTATC TGAAGGCAGA GCAGGAGCGG CAGCACAATT ACCTGAAGGA TGGGCCTTAC 
ATCACAGCCG AGGAGGCAGT GGCCGTGTAT ACCACCACTG TGCACTGGCT GGAGAGCCGG 
AGATTCTCGC CCATCCCCTT CCCTCCACTC TCCTACAAGC ATGATACCAA GCTGCTCATC 
TTGGCTCTGG AGCGTCTCAA GGAAGCTTAT AGTGTGAAGT CTAGGTTAAA CCAGTCTCAG 
AGGGAGGAGC TGGGTCTGAT TGAACAAGCC TATGACAACC CCCATGAGGC TTTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC ACAGAGAGCC TTTAAGGAAG TGGGCATTGA GTTTATGGAT 
CTCTATAGCC ACCTTGTACC AGTGTATGAT GTGGAACCAC TGGAGAAAAT AACTGATGCT 
TACCTGGACC AGTACCTGTG GTATGAAGCA GATAAACGCC GTCTTTTCCC ACCCTGGATC 
AAGCCTGCTG ACACAGAGCC ACCTCCACTG CTTGTTTACA AGTGGTGTCA AGGCATTAAT 
AACCTGCAGG ATGTGTGGGA GACGAGTGAG GGCGAGTGCA ACGTCATGCT GGAGTCTCGG 
TTTGAGAAGA TGTATGAGAA GATTGACCTG ACCCTGCTTA ACAGGCTGCT GCGACTCATT 
GTGGACCACA ACATAGCTGA CTACATGACG GCCAAGAACA ACGTAGTCAT CAACTATAAG 
GACATGAACC ATACAAACTC ATATGGAATC ATCAGAGGCC TGCAGTTTGC CTCATTCATT 
GTACAGTACT ATGGCTTGGT GATGGATTTG CTTGTACTGG GATTGCACCG GGCCAGTGAG 
ATGGCTGGGC CTCCTCAGAT GCCAAATGAC TTTCTCAGTT TCCAGGACAT AGCCACTGAG 
GCTGCACACC CAATCCGACT CTTCTGTAGA TACATTGATC GCATCCATAT TTTCTTCAGG 
TTCACAGCAG ATGAGGCTCG GGATCTGATC CAGCGTTACC TGACAGAACA CCCAGACCCC 
AACAATGAAA ACATTGTTGG CTACAATAAC AAGAAATGCT GGCCCCGAGA TGCCCGAATG 
CGGCTCATGA AGCATGATGT CAACTTGGGT CGGGCAGTGT TCTGGGACAT CAAAAACCGG 
CTGCCACGAT CGGTGACTAC AGTACAGTGG GAGAATAGCT TTGTGTCTGT GTACAGTAAA 
GACAACCCCA ACCTGCTATT CAACATGTGT GGCTTTGAGT GCCGCATCCT GCCTAAGTGC 
CGTACCAGCT ATGAAGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAATGAGGTT 
ACCAAGGAGC GGACAGCTCA GTGCTTCCTG CGTGTGGATG ATGAGTCAAT GCAACGCTTC 
CATAACCGTG TGCGGCAGAT TCTGATGGCC TCTGGCTCCA CCACGTTCAC CAAGATTGTG 
AATAAGTGGA ACACAGCTCT CATCGGCCTT ATGACATATT TTCGAGAGGC TGTGGTGAAT 
ACACAGGAAC TCCTGGACTT ATTGGTGAAG TGTGAAAACA AGATCCAGAC ACGTATCAAG 
ATTGGATTGA ACTCCAAGAT GCCTAGCCGG TTCCCGCCTG TTGTATTCTA CACCCCTAAG 
GAGCTGGGTG GGCTTGGCAT GCTCTCGATG GGCCATGTGC TTATCCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTGGGCATC ACACACTTCC GTTCAGGAAT GAGTCATGAA 
GAAGACCAAC TGATTCCCAA CTTGTACCGC TACATACAGC CATGGGAGAG CGAGTTCATT 
GACTCCCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCTAT TGCTCAGAAC 
AGACGCTTGA CTTTAGAAGA TTTAGAAGAT TCATGGGATC GAGGCATTCC CCGGATCAAT 
ACCCTCTTCC AGAAAGACCG GCACACATTG GCTTATGATA AAGGCTGGCG TGTCCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AACCCCTTTT GGTGGACACA TCAGCGGCAT 
GATGGCAAGC TTTGGAACCT CAATAACTAC CGCACAGACA TGATTCAGGC GCTGGGTGGT 
GTGGAGGGCA TTCTGGAGCA CACTCTCTTC AAGGGCACTT ACTTCCCCAC CTGGGAGGGT 
CTGTTCTGGG AGAAAGCCAG TGGCTTTGAG GAGTCCATGA AGTGGAAGAA GCTGACCAAT 
GCTCAGCGAT CAGGATTAAA CCAGATTCCT AATCGTAGAT TTACCCTCTG GTGGTCCCCA 
ACCATCAATC GAGCCAATGT CTATGTGGGC TTCCAGGTGC AGCTGGACCT GACAGGAATC 
TTCATGCACG GCAAGATCCC CACACTGAAG ATCTCTCTCA TCCAGATATT CCGAGCTCAC 
TTGTGGCAGA AGATCCATGA GAGCATCGTG ATGGACTTGT GTCAGGTCTT TGACCAGGAA 
CTGGATGCAC TGGAAATTGA GACAGTGCAA AAGGAGACAA TCCATCCCCG AAAGTCCTAT 
AAGATGAACT CTTCCTGTGC GGATATACTA CTCTTTGCCT CCTATAAGTG GAATGTCTCC 
CGGCCCTCGC TGCTGGCAGA CTCCAAGGAT GTGATGGACA GCACCACCAC ACAGAAGTAT 
TGGATTGACA TCCAATTACG CTGGGGAGAC TATGATTCCC ATGATATTGA GCGCTATGCC 
CGGGCCAAGT TCCTGGATTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACAGGC 
GTGCTCATTG CCATTGATCT GGCCTATAAT CTACACAGTG CCTATGGAAA CTGGTTCCCG 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCGTTG 
TATGTGTTAC GTGAACGGAT ACGCAAAGGA TTACAGCTGT ATTCATCTGA GCCCACTGAG 
CCTTACCTGT CCTCTCAGAA CTATGGTGAA CTCTTCTCTA ACCAGATTAT CTGGTTTGTG 
GATGACACCA ATGTCTATAG AGTAACTATC CACAAGACCT TTGAGGGGAA CTTGACAACT 
AAACCCATCA ATGGGGCCAT CTTCATCTTC AATCCACGAA CGGGACAGCT GTTCCTCAAG 
ATAATCCACA CATCTGTATG GGCTGGACAG AAGCGTTTGG GTCAGTTGGC CAAGTGGAAG 
ACTGCTGAGG AAGTGGCTGC CCTGATCCGA TCTCTGCCTG TGGAGGAACA GCCTAAACAG 
ATCATTGTCA CTCGGAAGGG CATGCTGGAT CCTCTGGAGG TCCACTTACT GGATTTCCCC 
AATATTGTGA TCAAAGGATC GGAGCTCCAG CTGCCCTTCC AGGCTTGTCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACC GAGCCCCAGA TGGTCCTTTT CAACCTCTAT 
GATGACTGGC TCAAGACTAT CTCCTCCTAC ACGGCTTTCT CCCGTCTCAT TCTGATTCTG 
CGGGCTTTGC ATGTGAACAA TGATAGAGCA AAGGTGATCC TGAAGCCAGA CAAGACTACT 
GTTACGGAAC CCCACCACAT CTGGCCCACG CTGACTGACG AGGAATGGAT CAAGGTGGAG 
GTACAGCTCA AGGATCTGAT CTTGGCTGAC TACGGCAAGA AAAACAACGT GAATGTGGCG 
TCACTGACAC AGTCAGAAAT TCGAGACATC ATCCTGGGAA TGGAGATCTC GGCGCCATCG 
CAGCAGCGGC AGCAGATAGC TGAGATTGAA AAGCAGACCA AGGAACAGTC CCAGCTCACT 
GCTACACAGA CTCGAACTGT CAACAAGCAC GGTGACGAGA TTATCACTTC AACCACTAGC 
AACTATGAGA CCCAGACCTT CTCATCAAAG ACTGAATGGA GAGTCAGGGC CATCTCTGCT 
GCCAACCTGC ATCTGCGGAC CAATCATATT TATGTTTCAT CTGATGACAT TAAGGAGACT 
GGTTATACGT ACATTCTTCC GAAAAACGTG CTTAAGAAGT TCATCTGCAT ATCTGATCTT 
CGTGCCCAAA TTGCAGGATA CCTGTATGGG GTGAGCCCTC CAGATAACCC TCAGGTGAAG 
GAGATCCGTT GTATTGTGAT GGTGCCGCAG TGGGGCACGC ACCAGACTGT GCACCTGCCC 
AGCCAGCTGC CCCAGCATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAGCCCAATG AATCCCCCCA GCTATCACCC CAAGATGTCA CCACTCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGGGAGAAG ACTATTATCA TCACGTGCAG CTTCACACCA 
GGCTCCTGCA CACTGACAGC CTACAAGCTG ACACCCAGTG GGTATGAGTG GGGCCGGCAG 
AACACAGACA AAGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTTCAG 
ATGCTCCTGT CTGACCGATT CCTCGGCTTC TTCATGGTCC CCGCCCAGTC CTCCTGGAAC 
TACAACTTTA TGGGTGTTCG ACATGATCCC AACATGAAAT ACGAGCTGCA GCTGGCAAAC 
CCCAAGGAAT TCTACCATGA GGTCCACAGG CCCTCCCACT TCCTCAACTT CGCCCTTCTG 
CAGGAGGGTG AGGTCTACTC TGCAGACAGA GAGGACCTCT ATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619600.2
CDS	316..7323
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTVTEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPSQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	NM_138659.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus pre-mRNA processing factor 8 (Prpf8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138659.2

ORF Insert Sequence:

ATGGCCGGAG TGTTTCCTTA CCGAGGGCCG GGTAACCCGG TGCCCGGCCC TCTAGCCCCG 
CTGCCGGATT ATATGTCAGA GGAGAAGCTG CAGGAGAAAG CTCGAAAATG GCAGCAGTTG 
CAGGCCAAGC GATATGCAGA GAAGCGGAAA TTTGGGTTTG TGGATGCTCA GAAGGAAGAC 
ATGCCACCAG AACATGTCAG GAAGATCATT CGAGACCATG GAGACATGAC CAACAGGAAG 
TTCCGTCATG ACAAAAGGGT CTATTTGGGA GCTCTTAAGT ACATGCCTCA TGCAGTCCTC 
AAGCTCCTTG AGAACATGCC CATGCCTTGG GAGCAGATCC GGGACGTGCC TGTGCTGTAC 
CACATCACTG GAGCCATCTC CTTTGTTAAC GAGATTCCCT GGGTTATCGA GCCGGTTTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATCATG ATGCGTAGAG AAAAAAGAGA CAGGAGGCAT 
TTCAAGAGGA TGCGTTTCCC CCCTTTCGAT GACGAGGAAC CGCCCCTGGA TTATGCCGAC 
AACATCTTAG ATGTTGAGCC ACTGGAAGCC ATTCAGCTAG AGCTGGACCC AGAGGAGGAT 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCTTTGA GAGACAGTAG GAAGTACGTT 
AATGGTTCCA CCTACCAACG CTGGCAGTTC ACATTGCCTA TGATGTCCAC ACTCTACCGA 
CTGGCCAATC AGCTTCTGAC TGACCTTGTA GATGACAACT ACTTCTATCT CTTTGATCTG 
AAGGCCTTTT TTACCTCTAA GGCACTCAAC ATGGCCATTC CTGGGGGACC CAAATTTGAG 
CCTCTTGTTC GAGACATCAA CTTACAGGAT GAAGACTGGA ATGAATTTAA CGATATTAAT 
AAGATCATCA TCCGTCAGCC TATTCGTACT GAGTACAAGA TTGCTTTTCC TTACCTGTAT 
AACAACCTTC CACACCACGT CCACCTGACC TGGTACCACA CTCCCAACGT TGTGTTCATC 
AAGACTGAGG ATCCTGATCT GCCAGCTTTC TACTTTGATC CTTTGATCAA CCCAATCTCC 
CATAGACACT CAGTCAAGAG CCAAGAACCA CTGCCTGATG ATGATGAAGA GTTTGAACTC 
CCAGAGTTTG TCGAGCCCTT CCTGAAGGAT ACACCTCTCT ATACAGACAA CACAGCCAAT 
GGCATTGCCC TGCTCTGGGC ACCACGGCCC TTCAACCTAC GCTCTGGTCG TACCCGCCGG 
GCCTTGGACA TACCCCTTGT CAAGAACTGG TATCGGGAGC ACTGTCCTGC TGGGCAGCCT 
GTGAAGGTTC GAGTCTCTTA TCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTCAAG 
CACCGGCCTC CCAAAGCCCA GAAGAAGAGG TATCTGTTCC GTTCTTTCAA AGCTACCAAA 
TTCTTTCAGT CCACCAAGCT GGACTGGGTG GAGGTAGGGC TGCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGG AAGAACCTCA ACTATCTGCA CTTGGACTAC 
AACTTCAACC TGAAGCCTGT CAAGACGCTC ACCACCAAGG AAAGAAAGAA ATCCCGTTTT 
GGGAATGCTT TCCATCTATG TCGGGAAGTA CTGCGTTTAA CCAAGCTGGT TGTGGACAGC 
CATGTGCAGT ATCGCTTGGG CAATGTAGAT GCCTTCCAGC TGGCAGATGG TTTGCAGTAT 
ATATTTGCCC ACGTGGGACA GTTGACAGGC ATGTATCGAT ACAAATATAA GCTGATGCGG 
CAGATCCGCA TGTGCAAGGA CTTGAAACAT CTCATCTATT ACCGCTTCAA CACGGGTCCT 
GTAGGGAAAG GCCCTGGCTG TGGTTTCTGG GCTGCTGGCT GGCGGGTCTG GCTGTTCTTT 
ATGCGTGGAA TTACCCCTCT GCTAGAGCGA TGGCTGGGCA ACCTTCTGGC CCGACAGTTT 
GAAGGCCGTC ACTCAAAGGG GGTGGCAAAG ACAGTAACGA AGCAGCGGGT GGAGTCGCAC 
TTTGACCTGG AGCTCAGGGC TGCTGTGATG CACGACATTC TGGACATGAT GCCTGAAGGC 
ATCAAGCAGA ACAAGGCCCG GACAATTCTG CAGCACCTCA GTGAAGCCTG GCGCTGCTGG 
AAGGCCAACA TCCCTTGGAA GGTCCCAGGA CTGCCAACAC CTATAGAGAA TATGATTCTT 
CGGTACGTTA AGGCCAAGGC TGATTGGTGG ACCAATACTG CCCACTACAA CCGGGAACGG 
ATCCGTCGCG GGGCTACTGT AGACAAGACT GTTTGCAAAA AGAACCTGGG CCGCCTCACT 
CGCCTGTATC TGAAGGCAGA GCAGGAGCGG CAGCACAATT ACCTGAAGGA TGGGCCTTAC 
ATCACAGCCG AGGAGGCAGT GGCCGTGTAT ACCACCACTG TGCACTGGCT GGAGAGCCGG 
AGATTCTCGC CCATCCCCTT CCCTCCACTC TCCTACAAGC ATGATACCAA GCTGCTCATC 
TTGGCTCTGG AGCGTCTCAA GGAAGCTTAT AGTGTGAAGT CTAGGTTAAA CCAGTCTCAG 
AGGGAGGAGC TGGGTCTGAT TGAACAAGCC TATGACAACC CCCATGAGGC TTTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC ACAGAGAGCC TTTAAGGAAG TGGGCATTGA GTTTATGGAT 
CTCTATAGCC ACCTTGTACC AGTGTATGAT GTGGAACCAC TGGAGAAAAT AACTGATGCT 
TACCTGGACC AGTACCTGTG GTATGAAGCA GATAAACGCC GTCTTTTCCC ACCCTGGATC 
AAGCCTGCTG ACACAGAGCC ACCTCCACTG CTTGTTTACA AGTGGTGTCA AGGCATTAAT 
AACCTGCAGG ATGTGTGGGA GACGAGTGAG GGCGAGTGCA ACGTCATGCT GGAGTCTCGG 
TTTGAGAAGA TGTATGAGAA GATTGACCTG ACCCTGCTTA ACAGGCTGCT GCGACTCATT 
GTGGACCACA ACATAGCTGA CTACATGACG GCCAAGAACA ACGTAGTCAT CAACTATAAG 
GACATGAACC ATACAAACTC ATATGGAATC ATCAGAGGCC TGCAGTTTGC CTCATTCATT 
GTACAGTACT ATGGCTTGGT GATGGATTTG CTTGTACTGG GATTGCACCG GGCCAGTGAG 
ATGGCTGGGC CTCCTCAGAT GCCAAATGAC TTTCTCAGTT TCCAGGACAT AGCCACTGAG 
GCTGCACACC CAATCCGACT CTTCTGTAGA TACATTGATC GCATCCATAT TTTCTTCAGG 
TTCACAGCAG ATGAGGCTCG GGATCTGATC CAGCGTTACC TGACAGAACA CCCAGACCCC 
AACAATGAAA ACATTGTTGG CTACAATAAC AAGAAATGCT GGCCCCGAGA TGCCCGAATG 
CGGCTCATGA AGCATGATGT CAACTTGGGT CGGGCAGTGT TCTGGGACAT CAAAAACCGG 
CTGCCACGAT CGGTGACTAC AGTACAGTGG GAGAATAGCT TTGTGTCTGT GTACAGTAAA 
GACAACCCCA ACCTGCTATT CAACATGTGT GGCTTTGAGT GCCGCATCCT GCCTAAGTGC 
CGTACCAGCT ATGAAGAGTT CACCCACAAG GACGGGGTCT GGAACCTACA GAATGAGGTT 
ACCAAGGAGC GGACAGCTCA GTGCTTCCTG CGTGTGGATG ATGAGTCAAT GCAACGCTTC 
CATAACCGTG TGCGGCAGAT TCTGATGGCC TCTGGCTCCA CCACGTTCAC CAAGATTGTG 
AATAAGTGGA ACACAGCTCT CATCGGCCTT ATGACATATT TTCGAGAGGC TGTGGTGAAT 
ACACAGGAAC TCCTGGACTT ATTGGTGAAG TGTGAAAACA AGATCCAGAC ACGTATCAAG 
ATTGGATTGA ACTCCAAGAT GCCTAGCCGG TTCCCGCCTG TTGTATTCTA CACCCCTAAG 
GAGCTGGGTG GGCTTGGCAT GCTCTCGATG GGCCATGTGC TTATCCCCCA GTCTGACCTC 
AGGTGGTCCA AGCAGACAGA TGTGGGCATC ACACACTTCC GTTCAGGAAT GAGTCATGAA 
GAAGACCAAC TGATTCCCAA CTTGTACCGC TACATACAGC CATGGGAGAG CGAGTTCATT 
GACTCCCAGC GGGTCTGGGC TGAGTATGCA CTCAAGAGAC AAGAGGCTAT TGCTCAGAAC 
AGACGCTTGA CTTTAGAAGA TTTAGAAGAT TCATGGGATC GAGGCATTCC CCGGATCAAT 
ACCCTCTTCC AGAAAGACCG GCACACATTG GCTTATGATA AAGGCTGGCG TGTCCGAACT 
GACTTTAAGC AGTACCAGGT TTTGAAGCAG AACCCCTTTT GGTGGACACA TCAGCGGCAT 
GATGGCAAGC TTTGGAACCT CAATAACTAC CGCACAGACA TGATTCAGGC GCTGGGTGGT 
GTGGAGGGCA TTCTGGAGCA CACTCTCTTC AAGGGCACTT ACTTCCCCAC CTGGGAGGGT 
CTGTTCTGGG AGAAAGCCAG TGGCTTTGAG GAGTCCATGA AGTGGAAGAA GCTGACCAAT 
GCTCAGCGAT CAGGATTAAA CCAGATTCCT AATCGTAGAT TTACCCTCTG GTGGTCCCCA 
ACCATCAATC GAGCCAATGT CTATGTGGGC TTCCAGGTGC AGCTGGACCT GACAGGAATC 
TTCATGCACG GCAAGATCCC CACACTGAAG ATCTCTCTCA TCCAGATATT CCGAGCTCAC 
TTGTGGCAGA AGATCCATGA GAGCATCGTG ATGGACTTGT GTCAGGTCTT TGACCAGGAA 
CTGGATGCAC TGGAAATTGA GACAGTGCAA AAGGAGACAA TCCATCCCCG AAAGTCCTAT 
AAGATGAACT CTTCCTGTGC GGATATACTA CTCTTTGCCT CCTATAAGTG GAATGTCTCC 
CGGCCCTCGC TGCTGGCAGA CTCCAAGGAT GTGATGGACA GCACCACCAC ACAGAAGTAT 
TGGATTGACA TCCAATTACG CTGGGGAGAC TATGATTCCC ATGATATTGA GCGCTATGCC 
CGGGCCAAGT TCCTGGATTA CACCACAGAC AACATGAGTA TCTACCCTTC GCCCACAGGC 
GTGCTCATTG CCATTGATCT GGCCTATAAT CTACACAGTG CCTATGGAAA CTGGTTCCCG 
GGCAGCAAGC CTCTCATACA GCAGGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCGTTG 
TATGTGTTAC GTGAACGGAT ACGCAAAGGA TTACAGCTGT ATTCATCTGA GCCCACTGAG 
CCTTACCTGT CCTCTCAGAA CTATGGTGAA CTCTTCTCTA ACCAGATTAT CTGGTTTGTG 
GATGACACCA ATGTCTATAG AGTAACTATC CACAAGACCT TTGAGGGGAA CTTGACAACT 
AAACCCATCA ATGGGGCCAT CTTCATCTTC AATCCACGAA CGGGACAGCT GTTCCTCAAG 
ATAATCCACA CATCTGTATG GGCTGGACAG AAGCGTTTGG GTCAGTTGGC CAAGTGGAAG 
ACTGCTGAGG AAGTGGCTGC CCTGATCCGA TCTCTGCCTG TGGAGGAACA GCCTAAACAG 
ATCATTGTCA CTCGGAAGGG CATGCTGGAT CCTCTGGAGG TCCACTTACT GGATTTCCCC 
AATATTGTGA TCAAAGGATC GGAGCTCCAG CTGCCCTTCC AGGCTTGTCT CAAGGTGGAG 
AAGTTTGGGG ATCTCATCCT TAAAGCCACC GAGCCCCAGA TGGTCCTTTT CAACCTCTAT 
GATGACTGGC TCAAGACTAT CTCCTCCTAC ACGGCTTTCT CCCGTCTCAT TCTGATTCTG 
CGGGCTTTGC ATGTGAACAA TGATAGAGCA AAGGTGATCC TGAAGCCAGA CAAGACTACT 
GTTACGGAAC CCCACCACAT CTGGCCCACG CTGACTGACG AGGAATGGAT CAAGGTGGAG 
GTACAGCTCA AGGATCTGAT CTTGGCTGAC TACGGCAAGA AAAACAACGT GAATGTGGCG 
TCACTGACAC AGTCAGAAAT TCGAGACATC ATCCTGGGAA TGGAGATCTC GGCGCCATCG 
CAGCAGCGGC AGCAGATAGC TGAGATTGAA AAGCAGACCA AGGAACAGTC CCAGCTCACT 
GCTACACAGA CTCGAACTGT CAACAAGCAC GGTGACGAGA TTATCACTTC AACCACTAGC 
AACTATGAGA CCCAGACCTT CTCATCAAAG ACTGAATGGA GAGTCAGGGC CATCTCTGCT 
GCCAACCTGC ATCTGCGGAC CAATCATATT TATGTTTCAT CTGATGACAT TAAGGAGACT 
GGTTATACGT ACATTCTTCC GAAAAACGTG CTTAAGAAGT TCATCTGCAT ATCTGATCTT 
CGTGCCCAAA TTGCAGGATA CCTGTATGGG GTGAGCCCTC CAGATAACCC TCAGGTGAAG 
GAGATCCGTT GTATTGTGAT GGTGCCGCAG TGGGGCACGC ACCAGACTGT GCACCTGCCC 
AGCCAGCTGC CCCAGCATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAGCCCAATG AATCCCCCCA GCTATCACCC CAAGATGTCA CCACTCATGC CAAAATCATG 
GCTGACAACC CATCTTGGGA TGGGGAGAAG ACTATTATCA TCACGTGCAG CTTCACACCA 
GGCTCCTGCA CACTGACAGC CTACAAGCTG ACACCCAGTG GGTATGAGTG GGGCCGGCAG 
AACACAGACA AAGGCAACAA CCCCAAAGGC TACCTACCCT CGCACTACGA GAGGGTTCAG 
ATGCTCCTGT CTGACCGATT CCTCGGCTTC TTCATGGTCC CCGCCCAGTC CTCCTGGAAC 
TACAACTTTA TGGGTGTTCG ACATGATCCC AACATGAAAT ACGAGCTGCA GCTGGCAAAC 
CCCAAGGAAT TCTACCATGA GGTCCACAGG CCCTCCCACT TCCTCAACTT CGCCCTTCTG 
CAGGAGGGTG AGGTCTACTC TGCAGACAGA GAGGACCTCT ATGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in pre-mRNA processing factors 3, 8, and 31 cause dysfunction of the retinal pigment epithelium.
The American journal of pathology184(10)2641-52(2014 Oct)
Farkas MH,Lew DS,Sousa ME,Bujakowska K,Chatagnon J,Bhattacharya SS,Pierce EA,Nandrot EF

Erbb2 is required for cardiac atrial electrical activity during development.
PloS one9(9)e107041(2014)
Tenin G,Clowes C,Wolton K,Krejci E,Wright JA,Lovell SC,Sedmera D,Hentges KE

Three gene-targeted mouse models of RNA splicing factor RP show late-onset RPE and retinal degeneration.
Investigative ophthalmology & visual science52(1)190-8(2011 Jan)
Graziotto JJ,Farkas MH,Bujakowska K,Deramaudt BM,Zhang Q,Nandrot EF,Inglehearn CF,Bhattacharya SS,Pierce EA

Temporal and tissue specific regulation of RP-associated splicing factor genes PRPF3, PRPF31 and PRPC8--implications in the pathogenesis of RP.
PloS one6(1)e15860(2011 Jan)
Cao H,Wu J,Lam S,Duan R,Newnham C,Molday RS,Graziotto JJ,Pierce EA,Hu J