gRNAs
The following prpf8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prpf8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ODa02004 | |
| Clone ID Related Accession (Same CDS sequence) | NM_200976.2 , XM_005157646.3 , XM_005157646.4 | |
| Accession Version | NM_200976.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 7029bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-11-23 | |
| Organism | Danio rerio(zebrafish) | |
| Product | pre-mRNA-processing-splicing factor 8 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR522882.5. On or before Mar 12, 2008 this sequence version replaced XM_694534.2, NM_200976.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01000188.1, GFIL01031529.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGCAGCTC CTTTCCCCTA CAGAGGTCTT CCTGGACCAA TGCCTCCAGG TGTTCCTCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_957270.2 |
| CDS | 131..7159 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_200976.2 |
1 | ATGGCAGCTC CTTTCCCCTA CAGAGGTCTT CCTGGACCAA TGCCTCCAGG TGTTCCTCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa02004 | |
| Clone ID Related Accession (Same CDS sequence) | NM_200976.2 , XM_005157646.3 , XM_005157646.4 | |
| Accession Version | XM_005157646.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 7029bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-23 | |
| Organism | Danio rerio(Zebrafish) | |
| Product | pre-mRNA-processing-splicing factor 8 isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157646.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCAGCTC CTTTCCCCTA CAGAGGTCTT CCTGGACCAA TGCCTCCAGG TGTTCCTCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005157703.1 |
| CDS | 112..7140 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005157646.3 |
1 | ATGGCAGCTC CTTTCCCCTA CAGAGGTCTT CCTGGACCAA TGCCTCCAGG TGTTCCTCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa02004 | |
| Clone ID Related Accession (Same CDS sequence) | NM_200976.2 , XM_005157646.3 , XM_005157646.4 | |
| Accession Version | XM_005157646.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 7029bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-06-14 | |
| Organism | Danio rerio(zebrafish) | |
| Product | pre-mRNA-processing-splicing factor 8 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157646.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCAGCTC CTTTCCCCTA CAGAGGTCTT CCTGGACCAA TGCCTCCAGG TGTTCCTCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005157703.1 |
| CDS | 130..7158 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005157646.4 |
1 | ATGGCAGCTC CTTTCCCCTA CAGAGGTCTT CCTGGACCAA TGCCTCCAGG TGTTCCTCCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
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