prp-8 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	prp-8
Entrez Gene ID	176153
Full Name	Pre-mRNA-splicing factor 8 homolog
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_066384.5	NP_498785.1	Pre-mRNA-splicing factor 8 homolog
NM_066384.4	NP_498785.1	Pre-mRNA-splicing factor 8 homolog

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prpf8	NP_001178519.1
Neurospora crassa	NCU07832	XP_962953.1
Arabidopsis thaliana (thale cress)	SUS2	NP_178124.2
	PRPF8	XP_001117328.1
Canis lupus familiaris (dog)	PRPF8	XP_854384.1
Drosophila melanogaster (fruit fly)	Prp8	NP_610735.1
Caenorhabditis elegans (roundworm)	prp-8	NP_498785.1
Schizosaccharomyces pombe (fission yeast)	spp42	NP_593861.1
Magnaporthe oryzae (rice blast fungus)	MGG_05244	XP_003712798.1
Oryza sativa (rice)	Os06g0167000	NP_001056917.1
Homo sapiens (human)	PRPF8	NP_006436.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006885	XP_308873.2
Kluyveromyces lactis	KLLA0A05280g	XP_451233.1
Eremothecium gossypii	AGOS_ABR207W	NP_983156.2
Bos taurus (cattle)	PRPF8	NP_001099108.1
Mus musculus (house mouse)	Prpf8	NP_619600.2
Arabidopsis thaliana (thale cress)	AT4G38780	NP_195589.2
Xenopus tropicalis (tropical clawed frog)	prpf8	XP_002936105.1
Gallus gallus (chicken)	PRPF8	XP_415805.2
Danio rerio (zebrafish)	prpf8	NP_957270.2
Saccharomyces cerevisiae (baker's yeast)	PRP8	NP_012035.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following prp-8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prp-8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa341197	NM_066384.5 Latest version!	Caenorhabditis elegans Pre-mRNA-splicing factor 8 homolog (prp-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCa341197	NM_066384.4	Caenorhabditis elegans Pre-mRNA-splicing factor 8 homolog (prp-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prp-8 ( NM_066384.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498785.1 Caenorhabditis elegans Pre-mRNA-splicing factor 8 homolog (prp-8), mRNA.

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CloneID	OCa341197
Clone ID Related Accession (Same CDS sequence)	NM_066384.5 , NM_066384.4
Accession Version	NM_066384.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Pre-mRNA-splicing factor 8 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_066384.4.

ATGGCCAACT ACGGCGGTCA TCCCCAAACT GAACCACATG CTATCCCGGA TTCAATTCTG 
GAAGAAAAGT CTCGAAAATG GAAACAACTT CAAGGAAAGC GGTATTCGGA GAAAAAGAAG 
TTTGGAATGT CCGATACGCA GAAAGAAGAA ATGCCACCTG AGCACGTCCG AAAAGTTATC 
AGAGATCATG GTGATATGAC TTCTCGCAAA TATCGTCATG ATAAAAGAGT CTACCTCGGT 
GCTTTGAAAT ATATGCCACA TGCCGTTTTG AAGCTACTGG AGAACATGCC GATGCCCTGG 
GAACAAATCA GAGATGTAAA AGTTCTGTAT CACATCACCG GTGCGATCAC TTTTGTCAAC 
GACATTCCGC GTGTCATCGA GCCGGTCTAC ATGGCACAAT GGGGAACGAT GTGGATTATG 
ATGAGAAGAG AAAAACGTGA TCGTCGTCAT TTCAAGCGAA TGAGATTCCC TCCATTTGAC 
GATGAAGAAC CGCCTCTCGA TTATGCTGAT AATATTCTGG ATGTCGAGCC TTTAGAGCCA 
ATTCAAATGG AACTAGATCC AGAAGAAGAC GGAGCTGTTG CTGAATGGTT CTACGATCAC 
AAGCCTCTTG CAACAACTCG ATTTGTGAAC GGCCCAACGT ACAGAAAATG GGCATTCAGT 
ATTCCACAGA TGTCCACATT GTATCGTCTT GCAAATCAGC TGCTTACCGA TTTAGTAGAT 
GATAACTACT TCTACTTGTT TGACATGAAA AGTTTCTTCA CTGCTAAAGC ACTCAACGTT 
GCAATTCCAG GAGGCCCGAA GTTCGAACCA TTGGTAAAAG ATTTACATAC TGATGAAGAC 
TGGAACGAGT TCAATGATAT CAATAAAGTT ATTATTCGAG CTCCAATTCG TACCGAGTAC 
AGAATTGCAT TCCCATTCAT GTACAACAAT CTGATCAGCT CGCTGCCTGT TCAAGTTTCA 
TGGTATCATA CTCCTTCAGT TGTATTTATC AAAACTGAAG ACCCAGATCT TCCCGCATTC 
TACTATGATC CATTGATCAA TCCAATCGTT CTCAGCAACT TGAAAGCAAC TGAAGAAAAT 
CTACCAGAAG GTGAAGAGGA AGACGAGTGG GAGCTGCCTG AAGATGTTCG ACCCATTTTT 
GAAGACGTTC CACTCTACAC CGATAACACG GCTAATGGTT TAGCACTTCT ATGGGCCCCT 
CGGCCATTCA ATCTTCGATC GGGAAGAACG AGAAGAGCAG TGGATGTACC ACTTGTCAAA 
TCGTGGTATA GAGAACATTG TCCAGCAGGA ATGCCCGTGA AAGTTCGTGT TTCTTATCAG 
AAACTGCTCA AGGTGTTTGT TCTGAACGCT CTCAAACATC GTCCACCGAA GCCCCAGAAG 
CGTCGTTATC TGTTCCGTTC CTTCAAGGCA ACAAAATTCT TCCAAACAAC CACCCTGGAT 
TGGGTAGAAG CTGGATTGCA AGTTCTTCGT CAAGGATACA ACATGCTCAA TTTGCTCATT 
CACCGAAAGA ATCTCAACTA TCTTCATCTT GATTACAACT TCAATTTGAA ACCAGTTAAA 
ACATTGACAA CAAAGGAAAG AAAGAAATCT CGTTTCGGAA ATGCATTCCA TTTGTGCAGA 
GAAATCCTTC GTCTCACAAA ACTTGTTGTT GATGCCCATG TTCAGTATCG TTTGAACAAC 
GTCGATGCGT ACCAACTTGC AGATGGTCTA CAATATATCT TTGCTCACGT CGGTCAACTG 
ACCGGAATGT ATCGTTACAA GTACAAGCTC ATGAGACAAG TCAGAATGTG CAAGGATCTC 
AAGCATCTCA TCTACTATCG TTTCAACACT GGGCCGGTTG GTAAAGGACC TGGATGTGGT 
TTTTGGGCTC CGGGATGGAG AGTTTGGTTG TTCTTCTTGC GTGGAATCAC ACCACTGCTC 
GAGAGATGGC TTGGAAATCT GTTGTCTCGA CAATTCGAAG GAAGACATTC AAAGGGAGTG 
GCCAAGACAG TCACCAAGCA AAGAGTTGAA TCTCATTTCG ATCTTGAATT ACGTGCTGCT 
GTCATGCATG ACATTCTGGA TATGATGCCG GACGGAATCA AACAAAATAA GGCTCGTGTT 
ATTCTTCAGC ATTTGTCAGA GGCGTGGAGA TGTTGGAAGG CTAATATTCC ATGGAAGGTT 
CCCGGACTGC CAACTCCAGT TGAAAATATG ATTCTACGTT ATGTGAAAGC AAAGGCTGAT 
TGGTGGACTA ATTCGGCACA TTACAACAGA GAACGTGTAA GACGAGGAGC GACAGTAGAC 
AAAACTGTGT GCAAGAAGAA CTTGGGTAGA TTGACAAGAT TATACCTGAA ATCAGAGCAA 
GAAAGACAAC ACAATTACTT GAAGGACGGA CCATATATCT CTGCAGAAGA AGCTGTTGCA 
ATTTACACGA CTACAGTACA TTGGCTGGAA TCAAGAAGAT TTTCTCCGAT TCCTTTCCCA 
CCACTTTCTT ACAAACACGA CACAAAACTT CTTATTTTGG CTCTTGAAAG ACTGAAGGAA 
AGTTACAGTG TGAAGAATAG ATTGAATCAA TCCCAAAGAG AAGAACTGGC TTTAATTGAA 
CAGGCTTATG ATAATCCTCA CGAAGCTTTG TCTCGTATCA AGAGACACAT GCTCACGCAG 
CGTGCATTCA AAGAAGTCGG AATTGAGTTT ATGGATCTCT ACACTCATCT TATTCCAGTC 
TACGATATCG AGCCTCTTGA GAAAGTCACT GACGCTTACC TCGATCAATA CCTCTGGTAT 
GAAGCAGACA AGAGAAGATT GTTCCCGGCA TGGGTGAAAC CAGGAGATAC TGAACCACCA 
CCGCTTCTTA CATACAAATG GTGTCAAGGT CTCAACAATC TTCAAGATGT TTGGGAGACA 
AGTGAAGGAG AGTGTAATGT CATCATGGAA ACAAAATTGG AGAAAATTGC TGAAAAAATG 
GATTTGACAC TTCTCAATAG ATTGCTCAGA TTGATTGTGG ATCACAATAT TGCCGATTAC 
ATGACTTCGA AGAACAACGT TCTCATCAAC TATAAGGATA TGAACCACAC CAATTCTTTC 
GGAATTATTC GTGGTCTACA ATTTGCTTCG TTTATTGTTC AATTCTATGG ATTAGTTCTT 
GATCTTCTTG TTCTTGGACT TCGACGAGCT TCCGAAATTG CCGGTCCGCC CCAATGTCCA 
AACGAGTTCT TGCAATTCCA GGATGTTGCC ACAGAAATTG GTCACCCTAT TCGTCTCTAC 
TGCCGGTACA TCGACCGAGT TTGGATCATG TTCCGTTTCA GTGCTGATGA AGCACGTGAT 
TTGATTCAAA GATACTTGAC AGAGCATCCA GATCCGAATA ATGAGAATAT TGTCGGTTAT 
AACAACAAAA AGTGTTGGCC AAGAGACGCT AGAATGAGAT TGATGAAACA TGATGTCAAT 
TTGGGTCGTG CTGTCTTCTG GGATATCAAG AACCGTCTCC CACGTTCCAT CACAACTGTC 
GAGTGGGAAA ATTCATTCGT ATCAGTGTAC AGTAAGGATA ACCCGAACAT GTTGTTCGAC 
ATGTCCGGAT TCGAGTGTCG TATTCTACCA AAGTGTCGTA CGGCTAATGA AGAGTTTGTT 
CATCGTGATG GTGTATGGAA TCTTCAAAAC GAAGTGACAA AGGAAAGAAC TGCTCAGTGC 
TTCTTGAAGG TCGATGAGGA GTCATTGTCC AAATTCCATA ACAGAATTCG TCAAATTTTG 
ATGTCTTCTG GATCTACAAC GTTCACCAAA ATTGTCAACA AATGGAATAC AGCATTGATC 
GGATTGATGA CCTACTTCAG AGAAGCTGTT GTGAACACTC AAGAACTTTT GGATTTATTG 
GTGAAATGTG AGAATAAAAT TCAAACGAGA ATCAAGATTG GACTCAACTC AAAAATGCCC 
TCAAGATTCC CTCCAGTTGT TTTCTATACG CCAAAAGAAA TCGGAGGACT TGGAATGCTA 
TCTATGGGAC ACGTCTTGAT CCCACAGTCT GATCTTCGAT GGATGCAACA AACGGAAGCT 
GGAGGTGTCA CTCACTTCCG TTCCGGAATG AGTCACGATG AAGACCAATT GATTCCAAAT 
CTGTACAGAT ATATTCAACC TTGGGAAGCA GAGTTTGTCG ATTCTGTTCG TGTGTGGGCA 
GAATATGCGC TCAAGAGACA AGAAGCCAAC GCTCAAAATA GAAGACTCAC TTTGGAAGAT 
CTCGACGATT CATGGGATCG TGGAATTCCG AGAATCAATA CTTTGTTCCA AAAAGATCGT 
CACACATTGG CATACGATAA AGGATGGCGT GTTAGAACCG AATTCAAGGC TTATCAAATT 
CTGAAGCAAA ATCCATTCTG GTGGACACAT CAAAGACACG ACGGAAAGTT ATGGAACTTG 
AACAACTATA GAACAGACAT GATTCAAGCA CTTGGAGGAG TAGAAGGAAT TTTGGAACAT 
ACATTATTCA GGGGAACCTA CTTCCCAACA TGGGAAGGTT TGTTCTGGGA ACGTGCATCT 
GGATTCGAAG AGTCGATGAA ATTCAAGAAA CTGACAAACG CCCAACGTTC TGGACTTAAT 
CAAATTCCGA ATCGTCGTTT CACTCTCTGG TGGTCTCCAA CTATCAACCG TGCTAATGTC 
TACGTCGGTT TCCAAGTACA ACTCGATTTG ACTGGAATTT TCATGCACGG AAAGATCCCG 
ACGCTCAAGA TTTCTCTCAT TCAAATCTTC CGTGCTCACT TGTGGCAAAA GATTCACGAG 
TCAGTAGTTA TGGATCTGTG TCAAGTTTTC GATCAGGAAC TCGATGCACT TGAGATTCAA 
ACAGTCCAAA AAGAAACTAT CCATCCGAGA AAGTCTTACA AAATGAACAG TTCGTGTGCC 
GACGTACTTC TCTTTGCTCA ATACAAGTGG AATGTGTCTA GGCCTTCTTT GATGGCAGAC 
AGCAAAGATG TCATGGATAA CACCACAACT CAGAAATATT GGCTCGATGT GCAGCTTCGT 
TGGGGAGATT ATGATTCTCA CGACGTTGAA CGATACGCCC GTGCAAAATT CCTTGACTAT 
ACCACTGATA ACATGTCGAT TTATCCTTCA CCGACTGGAG TTCTCATCGC AATCGACCTC 
GCGTACAATC TATATTCTGC GTACGGAAAC TGGTTCCCAG GAATGAAACC ATTGATTCGA 
CAGGCGATGG CAAAGATCAT CAAAGCCAAT CCAGCCTTCT ATGTGCTGCG TGAACGTATT 
CGCAAGGGTC TTCAACTCTA CTCGTCGGAA CCAACTGAAC CTTATTTGAC AAGTCAAAAC 
TACGGAGAGT TGTTCAGTAA CCAAATTATC TGGTTCGTTG ATGATACCAA TGTCTACCGT 
GTCACCATTC ACAAGACATT CGAAGGTAAC CTAACGACCA AGCCAATTAA CGGAGCCATC 
TTCATCTTCA ATCCACGTAC TGGTCAGCTT TTCCTCAAGA TCATTCATAC ATCCGTATGG 
GCTGGACAGA AGCGTTTGTC GCAGTTGGCC AAGTGGAAAA CAGCAGAAGA AGTTGCTGCC 
CTCATTCGTT CGCTTCCAGT TGAAGAACAA CCACGCCAGA TCATTGTAAC AAGAAAAGCT 
ATGCTCGATC CATTGGAGGT GCATCTTCTC GATTTCCCGA ATATTGTCAT CAAGGGATCT 
GAGCTCATGT TACCATTCCA AGCTATCATG AAGGTTGAAA AATTCGGTGA TTTAATTCTC 
AAGGCCACCG AGCCACAGAT GGTGTTGTTC AATCTGTACG ATGATTGGCT CAAGACAATT 
TCTTCATACA CTGCTTTCTC TCGTGTCGTT CTTATCATGC GTGGAATGCA CATTAATCCA 
GATAAGACTA AAGTTATCTT GAAACCAGAC AAGACTACTA TCACCGAGCC TCATCATATT 
TGGCCCACAC TTTCTGACGA CGATTGGATC AAGGTTGAAT TGGCTCTGAA GGATATGATT 
CTGGCAGATT ATGGAAAGAA GAACAATGTG AATGTTGCTT CATTGACTCA ATCTGAAGTC 
AGAGATATTA TTCTTGGAAT GGAAATCTCT GCGCCAAGCC AACAACGTCA ACAGATTGCG 
GATATTGAGA AACAAACGAA AGAGCAGAGT CAAGTTACTG CAACGACTAC ACGAACTGTC 
AATAAACATG GAGATGAAAT TATCACAGCA ACTACATCGA ATTACGAAAC AGCATCGTTT 
GCTAGTCGTA CAGAATGGAG AGTTCGTGCT ATTTCATCCA CAAATCTTCA TCTTCGAACT 
CAACACATCT ATGTCAATTC CGATGATGTC AAGGATACAG GATATACTTA TATTCTTCCA 
AAGAATATCC TCAAGAAGTT CATCACTATC TCGGATCTTC GAACACAGAT TGCTGGATTC 
ATGTATGGTG TATCGCCACC TGATAATCCA CAAGTAAAGG AGATTCGGTG CATTGTCCTC 
GTTCCACAGA CTGGAAGTCA TCAGCAAGTC AATCTTCCAA CACAACTTCC GGATCATGAG 
CTTCTTCGCG ATTTTGAGCC ACTTGGATGG ATGCACACTC AACCAAATGA GTTGCCACAA 
TTGTCTCCAC AAGATGTCAC AACTCATGCC AAGCTATTGA CCGATAACAT CAGCTGGGAT 
GGAGAAAAGA CAGTGATGAT CACGTGCAGT TTCACTCCTG GATCTGTTTC TCTGACTGCA 
TATAAACTTA CACCATCGGG ATATGAATGG GGTAAAGCGA ATACCGACAA AGGAAATAAT 
CCAAAGGGAT ATATGCCAAC TCACTATGAG AAAGTGCAAA TGCTTCTATC TGATCGATTC 
CTTGGATACT TCATGGTTCC ATCAAACGGA GTGTGGAACT ACAATTTCCA AGGTCAACGT 
TGGTCGCCTG CAATGAAGTT CGATGTGTGT CTTTCTAATC CAAAGGAGTA CTATCATGAA 
GATCATCGGC CGGTTCACTT CCATAACTTT AAGGCATTCG ACGATCCACT TGGAACAGGA 
AGCGCCGATC GCGAGGATGC ATTTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498785.1
CDS	5..6994
Translation	MANYGGHPQTEPHAIPDSILEEKSRKWKQLQGKRYSEKKKFGMSDTQKEEMPPEHVRKVIRDHGDMTSRKYRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVKVLYHITGAITFVNDIPRVIEPVYMAQWGTMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEPIQMELDPEEDGAVAEWFYDHKPLATTRFVNGPTYRKWAFSIPQMSTLYRLANQLLTDLVDDNYFYLFDMKSFFTAKALNVAIPGGPKFEPLVKDLHTDEDWNEFNDINKVIIRAPIRTEYRIAFPFMYNNLISSLPVQVSWYHTPSVVFIKTEDPDLPAFYYDPLINPIVLSNLKATEENLPEGEEEDEWELPEDVRPIFEDVPLYTDNTANGLALLWAPRPFNLRSGRTRRAVDVPLVKSWYREHCPAGMPVKVRVSYQKLLKVFVLNALKHRPPKPQKRRYLFRSFKATKFFQTTTLDWVEAGLQVLRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREILRLTKLVVDAHVQYRLNNVDAYQLADGLQYIFAHVGQLTGMYRYKYKLMRQVRMCKDLKHLIYYRFNTGPVGKGPGCGFWAPGWRVWLFFLRGITPLLERWLGNLLSRQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPDGIKQNKARVILQHLSEAWRCWKANIPWKVPGLPTPVENMILRYVKAKADWWTNSAHYNRERVRRGATVDKTVCKKNLGRLTRLYLKSEQERQHNYLKDGPYISAEEAVAIYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKESYSVKNRLNQSQREELALIEQAYDNPHEALSRIKRHMLTQRAFKEVGIEFMDLYTHLIPVYDIEPLEKVTDAYLDQYLWYEADKRRLFPAWVKPGDTEPPPLLTYKWCQGLNNLQDVWETSEGECNVIMETKLEKIAEKMDLTLLNRLLRLIVDHNIADYMTSKNNVLINYKDMNHTNSFGIIRGLQFASFIVQFYGLVLDLLVLGLRRASEIAGPPQCPNEFLQFQDVATEIGHPIRLYCRYIDRVWIMFRFSADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSITTVEWENSFVSVYSKDNPNMLFDMSGFECRILPKCRTANEEFVHRDGVWNLQNEVTKERTAQCFLKVDEESLSKFHNRIRQILMSSGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKEIGGLGMLSMGHVLIPQSDLRWMQQTEAGGVTHFRSGMSHDEDQLIPNLYRYIQPWEAEFVDSVRVWAEYALKRQEANAQNRRLTLEDLDDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTEFKAYQILKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFRGTYFPTWEGLFWERASGFEESMKFKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESVVMDLCQVFDQELDALEIQTVQKETIHPRKSYKMNSSCADVLLFAQYKWNVSRPSLMADSKDVMDNTTTQKYWLDVQLRWGDYDSHDVERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLYSAYGNWFPGMKPLIRQAMAKIIKANPAFYVLRERIRKGLQLYSSEPTEPYLTSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLSQLAKWKTAEEVAALIRSLPVEEQPRQIIVTRKAMLDPLEVHLLDFPNIVIKGSELMLPFQAIMKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRVVLIMRGMHINPDKTKVILKPDKTTITEPHHIWPTLSDDDWIKVELALKDMILADYGKKNNVNVASLTQSEVRDIILGMEISAPSQQRQQIADIEKQTKEQSQVTATTTRTVNKHGDEIITATTSNYETASFASRTEWRVRAISSTNLHLRTQHIYVNSDDVKDTGYTYILPKNILKKFITISDLRTQIAGFMYGVSPPDNPQVKEIRCIVLVPQTGSHQQVNLPTQLPDHELLRDFEPLGWMHTQPNELPQLSPQDVTTHAKLLTDNISWDGEKTVMITCSFTPGSVSLTAYKLTPSGYEWGKANTDKGNNPKGYMPTHYEKVQMLLSDRFLGYFMVPSNGVWNYNFQGQRWSPAMKFDVCLSNPKEYYHEDHRPVHFHNFKAFDDPLGTGSADREDAFA

Target ORF information:

RefSeq Version	NM_066384.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Pre-mRNA-splicing factor 8 homolog (prp-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066384.5

ORF Insert Sequence:

ATGGCCAACT ACGGCGGTCA TCCCCAAACT GAACCACATG CTATCCCGGA TTCAATTCTG 
GAAGAAAAGT CTCGAAAATG GAAACAACTT CAAGGAAAGC GGTATTCGGA GAAAAAGAAG 
TTTGGAATGT CCGATACGCA GAAAGAAGAA ATGCCACCTG AGCACGTCCG AAAAGTTATC 
AGAGATCATG GTGATATGAC TTCTCGCAAA TATCGTCATG ATAAAAGAGT CTACCTCGGT 
GCTTTGAAAT ATATGCCACA TGCCGTTTTG AAGCTACTGG AGAACATGCC GATGCCCTGG 
GAACAAATCA GAGATGTAAA AGTTCTGTAT CACATCACCG GTGCGATCAC TTTTGTCAAC 
GACATTCCGC GTGTCATCGA GCCGGTCTAC ATGGCACAAT GGGGAACGAT GTGGATTATG 
ATGAGAAGAG AAAAACGTGA TCGTCGTCAT TTCAAGCGAA TGAGATTCCC TCCATTTGAC 
GATGAAGAAC CGCCTCTCGA TTATGCTGAT AATATTCTGG ATGTCGAGCC TTTAGAGCCA 
ATTCAAATGG AACTAGATCC AGAAGAAGAC GGAGCTGTTG CTGAATGGTT CTACGATCAC 
AAGCCTCTTG CAACAACTCG ATTTGTGAAC GGCCCAACGT ACAGAAAATG GGCATTCAGT 
ATTCCACAGA TGTCCACATT GTATCGTCTT GCAAATCAGC TGCTTACCGA TTTAGTAGAT 
GATAACTACT TCTACTTGTT TGACATGAAA AGTTTCTTCA CTGCTAAAGC ACTCAACGTT 
GCAATTCCAG GAGGCCCGAA GTTCGAACCA TTGGTAAAAG ATTTACATAC TGATGAAGAC 
TGGAACGAGT TCAATGATAT CAATAAAGTT ATTATTCGAG CTCCAATTCG TACCGAGTAC 
AGAATTGCAT TCCCATTCAT GTACAACAAT CTGATCAGCT CGCTGCCTGT TCAAGTTTCA 
TGGTATCATA CTCCTTCAGT TGTATTTATC AAAACTGAAG ACCCAGATCT TCCCGCATTC 
TACTATGATC CATTGATCAA TCCAATCGTT CTCAGCAACT TGAAAGCAAC TGAAGAAAAT 
CTACCAGAAG GTGAAGAGGA AGACGAGTGG GAGCTGCCTG AAGATGTTCG ACCCATTTTT 
GAAGACGTTC CACTCTACAC CGATAACACG GCTAATGGTT TAGCACTTCT ATGGGCCCCT 
CGGCCATTCA ATCTTCGATC GGGAAGAACG AGAAGAGCAG TGGATGTACC ACTTGTCAAA 
TCGTGGTATA GAGAACATTG TCCAGCAGGA ATGCCCGTGA AAGTTCGTGT TTCTTATCAG 
AAACTGCTCA AGGTGTTTGT TCTGAACGCT CTCAAACATC GTCCACCGAA GCCCCAGAAG 
CGTCGTTATC TGTTCCGTTC CTTCAAGGCA ACAAAATTCT TCCAAACAAC CACCCTGGAT 
TGGGTAGAAG CTGGATTGCA AGTTCTTCGT CAAGGATACA ACATGCTCAA TTTGCTCATT 
CACCGAAAGA ATCTCAACTA TCTTCATCTT GATTACAACT TCAATTTGAA ACCAGTTAAA 
ACATTGACAA CAAAGGAAAG AAAGAAATCT CGTTTCGGAA ATGCATTCCA TTTGTGCAGA 
GAAATCCTTC GTCTCACAAA ACTTGTTGTT GATGCCCATG TTCAGTATCG TTTGAACAAC 
GTCGATGCGT ACCAACTTGC AGATGGTCTA CAATATATCT TTGCTCACGT CGGTCAACTG 
ACCGGAATGT ATCGTTACAA GTACAAGCTC ATGAGACAAG TCAGAATGTG CAAGGATCTC 
AAGCATCTCA TCTACTATCG TTTCAACACT GGGCCGGTTG GTAAAGGACC TGGATGTGGT 
TTTTGGGCTC CGGGATGGAG AGTTTGGTTG TTCTTCTTGC GTGGAATCAC ACCACTGCTC 
GAGAGATGGC TTGGAAATCT GTTGTCTCGA CAATTCGAAG GAAGACATTC AAAGGGAGTG 
GCCAAGACAG TCACCAAGCA AAGAGTTGAA TCTCATTTCG ATCTTGAATT ACGTGCTGCT 
GTCATGCATG ACATTCTGGA TATGATGCCG GACGGAATCA AACAAAATAA GGCTCGTGTT 
ATTCTTCAGC ATTTGTCAGA GGCGTGGAGA TGTTGGAAGG CTAATATTCC ATGGAAGGTT 
CCCGGACTGC CAACTCCAGT TGAAAATATG ATTCTACGTT ATGTGAAAGC AAAGGCTGAT 
TGGTGGACTA ATTCGGCACA TTACAACAGA GAACGTGTAA GACGAGGAGC GACAGTAGAC 
AAAACTGTGT GCAAGAAGAA CTTGGGTAGA TTGACAAGAT TATACCTGAA ATCAGAGCAA 
GAAAGACAAC ACAATTACTT GAAGGACGGA CCATATATCT CTGCAGAAGA AGCTGTTGCA 
ATTTACACGA CTACAGTACA TTGGCTGGAA TCAAGAAGAT TTTCTCCGAT TCCTTTCCCA 
CCACTTTCTT ACAAACACGA CACAAAACTT CTTATTTTGG CTCTTGAAAG ACTGAAGGAA 
AGTTACAGTG TGAAGAATAG ATTGAATCAA TCCCAAAGAG AAGAACTGGC TTTAATTGAA 
CAGGCTTATG ATAATCCTCA CGAAGCTTTG TCTCGTATCA AGAGACACAT GCTCACGCAG 
CGTGCATTCA AAGAAGTCGG AATTGAGTTT ATGGATCTCT ACACTCATCT TATTCCAGTC 
TACGATATCG AGCCTCTTGA GAAAGTCACT GACGCTTACC TCGATCAATA CCTCTGGTAT 
GAAGCAGACA AGAGAAGATT GTTCCCGGCA TGGGTGAAAC CAGGAGATAC TGAACCACCA 
CCGCTTCTTA CATACAAATG GTGTCAAGGT CTCAACAATC TTCAAGATGT TTGGGAGACA 
AGTGAAGGAG AGTGTAATGT CATCATGGAA ACAAAATTGG AGAAAATTGC TGAAAAAATG 
GATTTGACAC TTCTCAATAG ATTGCTCAGA TTGATTGTGG ATCACAATAT TGCCGATTAC 
ATGACTTCGA AGAACAACGT TCTCATCAAC TATAAGGATA TGAACCACAC CAATTCTTTC 
GGAATTATTC GTGGTCTACA ATTTGCTTCG TTTATTGTTC AATTCTATGG ATTAGTTCTT 
GATCTTCTTG TTCTTGGACT TCGACGAGCT TCCGAAATTG CCGGTCCGCC CCAATGTCCA 
AACGAGTTCT TGCAATTCCA GGATGTTGCC ACAGAAATTG GTCACCCTAT TCGTCTCTAC 
TGCCGGTACA TCGACCGAGT TTGGATCATG TTCCGTTTCA GTGCTGATGA AGCACGTGAT 
TTGATTCAAA GATACTTGAC AGAGCATCCA GATCCGAATA ATGAGAATAT TGTCGGTTAT 
AACAACAAAA AGTGTTGGCC AAGAGACGCT AGAATGAGAT TGATGAAACA TGATGTCAAT 
TTGGGTCGTG CTGTCTTCTG GGATATCAAG AACCGTCTCC CACGTTCCAT CACAACTGTC 
GAGTGGGAAA ATTCATTCGT ATCAGTGTAC AGTAAGGATA ACCCGAACAT GTTGTTCGAC 
ATGTCCGGAT TCGAGTGTCG TATTCTACCA AAGTGTCGTA CGGCTAATGA AGAGTTTGTT 
CATCGTGATG GTGTATGGAA TCTTCAAAAC GAAGTGACAA AGGAAAGAAC TGCTCAGTGC 
TTCTTGAAGG TCGATGAGGA GTCATTGTCC AAATTCCATA ACAGAATTCG TCAAATTTTG 
ATGTCTTCTG GATCTACAAC GTTCACCAAA ATTGTCAACA AATGGAATAC AGCATTGATC 
GGATTGATGA CCTACTTCAG AGAAGCTGTT GTGAACACTC AAGAACTTTT GGATTTATTG 
GTGAAATGTG AGAATAAAAT TCAAACGAGA ATCAAGATTG GACTCAACTC AAAAATGCCC 
TCAAGATTCC CTCCAGTTGT TTTCTATACG CCAAAAGAAA TCGGAGGACT TGGAATGCTA 
TCTATGGGAC ACGTCTTGAT CCCACAGTCT GATCTTCGAT GGATGCAACA AACGGAAGCT 
GGAGGTGTCA CTCACTTCCG TTCCGGAATG AGTCACGATG AAGACCAATT GATTCCAAAT 
CTGTACAGAT ATATTCAACC TTGGGAAGCA GAGTTTGTCG ATTCTGTTCG TGTGTGGGCA 
GAATATGCGC TCAAGAGACA AGAAGCCAAC GCTCAAAATA GAAGACTCAC TTTGGAAGAT 
CTCGACGATT CATGGGATCG TGGAATTCCG AGAATCAATA CTTTGTTCCA AAAAGATCGT 
CACACATTGG CATACGATAA AGGATGGCGT GTTAGAACCG AATTCAAGGC TTATCAAATT 
CTGAAGCAAA ATCCATTCTG GTGGACACAT CAAAGACACG ACGGAAAGTT ATGGAACTTG 
AACAACTATA GAACAGACAT GATTCAAGCA CTTGGAGGAG TAGAAGGAAT TTTGGAACAT 
ACATTATTCA GGGGAACCTA CTTCCCAACA TGGGAAGGTT TGTTCTGGGA ACGTGCATCT 
GGATTCGAAG AGTCGATGAA ATTCAAGAAA CTGACAAACG CCCAACGTTC TGGACTTAAT 
CAAATTCCGA ATCGTCGTTT CACTCTCTGG TGGTCTCCAA CTATCAACCG TGCTAATGTC 
TACGTCGGTT TCCAAGTACA ACTCGATTTG ACTGGAATTT TCATGCACGG AAAGATCCCG 
ACGCTCAAGA TTTCTCTCAT TCAAATCTTC CGTGCTCACT TGTGGCAAAA GATTCACGAG 
TCAGTAGTTA TGGATCTGTG TCAAGTTTTC GATCAGGAAC TCGATGCACT TGAGATTCAA 
ACAGTCCAAA AAGAAACTAT CCATCCGAGA AAGTCTTACA AAATGAACAG TTCGTGTGCC 
GACGTACTTC TCTTTGCTCA ATACAAGTGG AATGTGTCTA GGCCTTCTTT GATGGCAGAC 
AGCAAAGATG TCATGGATAA CACCACAACT CAGAAATATT GGCTCGATGT GCAGCTTCGT 
TGGGGAGATT ATGATTCTCA CGACGTTGAA CGATACGCCC GTGCAAAATT CCTTGACTAT 
ACCACTGATA ACATGTCGAT TTATCCTTCA CCGACTGGAG TTCTCATCGC AATCGACCTC 
GCGTACAATC TATATTCTGC GTACGGAAAC TGGTTCCCAG GAATGAAACC ATTGATTCGA 
CAGGCGATGG CAAAGATCAT CAAAGCCAAT CCAGCCTTCT ATGTGCTGCG TGAACGTATT 
CGCAAGGGTC TTCAACTCTA CTCGTCGGAA CCAACTGAAC CTTATTTGAC AAGTCAAAAC 
TACGGAGAGT TGTTCAGTAA CCAAATTATC TGGTTCGTTG ATGATACCAA TGTCTACCGT 
GTCACCATTC ACAAGACATT CGAAGGTAAC CTAACGACCA AGCCAATTAA CGGAGCCATC 
TTCATCTTCA ATCCACGTAC TGGTCAGCTT TTCCTCAAGA TCATTCATAC ATCCGTATGG 
GCTGGACAGA AGCGTTTGTC GCAGTTGGCC AAGTGGAAAA CAGCAGAAGA AGTTGCTGCC 
CTCATTCGTT CGCTTCCAGT TGAAGAACAA CCACGCCAGA TCATTGTAAC AAGAAAAGCT 
ATGCTCGATC CATTGGAGGT GCATCTTCTC GATTTCCCGA ATATTGTCAT CAAGGGATCT 
GAGCTCATGT TACCATTCCA AGCTATCATG AAGGTTGAAA AATTCGGTGA TTTAATTCTC 
AAGGCCACCG AGCCACAGAT GGTGTTGTTC AATCTGTACG ATGATTGGCT CAAGACAATT 
TCTTCATACA CTGCTTTCTC TCGTGTCGTT CTTATCATGC GTGGAATGCA CATTAATCCA 
GATAAGACTA AAGTTATCTT GAAACCAGAC AAGACTACTA TCACCGAGCC TCATCATATT 
TGGCCCACAC TTTCTGACGA CGATTGGATC AAGGTTGAAT TGGCTCTGAA GGATATGATT 
CTGGCAGATT ATGGAAAGAA GAACAATGTG AATGTTGCTT CATTGACTCA ATCTGAAGTC 
AGAGATATTA TTCTTGGAAT GGAAATCTCT GCGCCAAGCC AACAACGTCA ACAGATTGCG 
GATATTGAGA AACAAACGAA AGAGCAGAGT CAAGTTACTG CAACGACTAC ACGAACTGTC 
AATAAACATG GAGATGAAAT TATCACAGCA ACTACATCGA ATTACGAAAC AGCATCGTTT 
GCTAGTCGTA CAGAATGGAG AGTTCGTGCT ATTTCATCCA CAAATCTTCA TCTTCGAACT 
CAACACATCT ATGTCAATTC CGATGATGTC AAGGATACAG GATATACTTA TATTCTTCCA 
AAGAATATCC TCAAGAAGTT CATCACTATC TCGGATCTTC GAACACAGAT TGCTGGATTC 
ATGTATGGTG TATCGCCACC TGATAATCCA CAAGTAAAGG AGATTCGGTG CATTGTCCTC 
GTTCCACAGA CTGGAAGTCA TCAGCAAGTC AATCTTCCAA CACAACTTCC GGATCATGAG 
CTTCTTCGCG ATTTTGAGCC ACTTGGATGG ATGCACACTC AACCAAATGA GTTGCCACAA 
TTGTCTCCAC AAGATGTCAC AACTCATGCC AAGCTATTGA CCGATAACAT CAGCTGGGAT 
GGAGAAAAGA CAGTGATGAT CACGTGCAGT TTCACTCCTG GATCTGTTTC TCTGACTGCA 
TATAAACTTA CACCATCGGG ATATGAATGG GGTAAAGCGA ATACCGACAA AGGAAATAAT 
CCAAAGGGAT ATATGCCAAC TCACTATGAG AAAGTGCAAA TGCTTCTATC TGATCGATTC 
CTTGGATACT TCATGGTTCC ATCAAACGGA GTGTGGAACT ACAATTTCCA AGGTCAACGT 
TGGTCGCCTG CAATGAAGTT CGATGTGTGT CTTTCTAATC CAAAGGAGTA CTATCATGAA 
GATCATCGGC CGGTTCACTT CCATAACTTT AAGGCATTCG ACGATCCACT TGGAACAGGA 
AGCGCCGATC GCGAGGATGC ATTTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prp-8 ( NM_066384.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498785.1 Caenorhabditis elegans Pre-mRNA-splicing factor 8 homolog (prp-8), partial mRNA.

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CloneID	OCa341197
Clone ID Related Accession (Same CDS sequence)	NM_066384.5 , NM_066384.4
Accession Version	NM_066384.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Pre-mRNA-splicing factor 8 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_066384.3. COMPLETENESS: incomplete on both ends.

ATGGCCAACT ACGGCGGTCA TCCCCAAACT GAACCACATG CTATCCCGGA TTCAATTCTG 
GAAGAAAAGT CTCGAAAATG GAAACAACTT CAAGGAAAGC GGTATTCGGA GAAAAAGAAG 
TTTGGAATGT CCGATACGCA GAAAGAAGAA ATGCCACCTG AGCACGTCCG AAAAGTTATC 
AGAGATCATG GTGATATGAC TTCTCGCAAA TATCGTCATG ATAAAAGAGT CTACCTCGGT 
GCTTTGAAAT ATATGCCACA TGCCGTTTTG AAGCTACTGG AGAACATGCC GATGCCCTGG 
GAACAAATCA GAGATGTAAA AGTTCTGTAT CACATCACCG GTGCGATCAC TTTTGTCAAC 
GACATTCCGC GTGTCATCGA GCCGGTCTAC ATGGCACAAT GGGGAACGAT GTGGATTATG 
ATGAGAAGAG AAAAACGTGA TCGTCGTCAT TTCAAGCGAA TGAGATTCCC TCCATTTGAC 
GATGAAGAAC CGCCTCTCGA TTATGCTGAT AATATTCTGG ATGTCGAGCC TTTAGAGCCA 
ATTCAAATGG AACTAGATCC AGAAGAAGAC GGAGCTGTTG CTGAATGGTT CTACGATCAC 
AAGCCTCTTG CAACAACTCG ATTTGTGAAC GGCCCAACGT ACAGAAAATG GGCATTCAGT 
ATTCCACAGA TGTCCACATT GTATCGTCTT GCAAATCAGC TGCTTACCGA TTTAGTAGAT 
GATAACTACT TCTACTTGTT TGACATGAAA AGTTTCTTCA CTGCTAAAGC ACTCAACGTT 
GCAATTCCAG GAGGCCCGAA GTTCGAACCA TTGGTAAAAG ATTTACATAC TGATGAAGAC 
TGGAACGAGT TCAATGATAT CAATAAAGTT ATTATTCGAG CTCCAATTCG TACCGAGTAC 
AGAATTGCAT TCCCATTCAT GTACAACAAT CTGATCAGCT CGCTGCCTGT TCAAGTTTCA 
TGGTATCATA CTCCTTCAGT TGTATTTATC AAAACTGAAG ACCCAGATCT TCCCGCATTC 
TACTATGATC CATTGATCAA TCCAATCGTT CTCAGCAACT TGAAAGCAAC TGAAGAAAAT 
CTACCAGAAG GTGAAGAGGA AGACGAGTGG GAGCTGCCTG AAGATGTTCG ACCCATTTTT 
GAAGACGTTC CACTCTACAC CGATAACACG GCTAATGGTT TAGCACTTCT ATGGGCCCCT 
CGGCCATTCA ATCTTCGATC GGGAAGAACG AGAAGAGCAG TGGATGTACC ACTTGTCAAA 
TCGTGGTATA GAGAACATTG TCCAGCAGGA ATGCCCGTGA AAGTTCGTGT TTCTTATCAG 
AAACTGCTCA AGGTGTTTGT TCTGAACGCT CTCAAACATC GTCCACCGAA GCCCCAGAAG 
CGTCGTTATC TGTTCCGTTC CTTCAAGGCA ACAAAATTCT TCCAAACAAC CACCCTGGAT 
TGGGTAGAAG CTGGATTGCA AGTTCTTCGT CAAGGATACA ACATGCTCAA TTTGCTCATT 
CACCGAAAGA ATCTCAACTA TCTTCATCTT GATTACAACT TCAATTTGAA ACCAGTTAAA 
ACATTGACAA CAAAGGAAAG AAAGAAATCT CGTTTCGGAA ATGCATTCCA TTTGTGCAGA 
GAAATCCTTC GTCTCACAAA ACTTGTTGTT GATGCCCATG TTCAGTATCG TTTGAACAAC 
GTCGATGCGT ACCAACTTGC AGATGGTCTA CAATATATCT TTGCTCACGT CGGTCAACTG 
ACCGGAATGT ATCGTTACAA GTACAAGCTC ATGAGACAAG TCAGAATGTG CAAGGATCTC 
AAGCATCTCA TCTACTATCG TTTCAACACT GGGCCGGTTG GTAAAGGACC TGGATGTGGT 
TTTTGGGCTC CGGGATGGAG AGTTTGGTTG TTCTTCTTGC GTGGAATCAC ACCACTGCTC 
GAGAGATGGC TTGGAAATCT GTTGTCTCGA CAATTCGAAG GAAGACATTC AAAGGGAGTG 
GCCAAGACAG TCACCAAGCA AAGAGTTGAA TCTCATTTCG ATCTTGAATT ACGTGCTGCT 
GTCATGCATG ACATTCTGGA TATGATGCCG GACGGAATCA AACAAAATAA GGCTCGTGTT 
ATTCTTCAGC ATTTGTCAGA GGCGTGGAGA TGTTGGAAGG CTAATATTCC ATGGAAGGTT 
CCCGGACTGC CAACTCCAGT TGAAAATATG ATTCTACGTT ATGTGAAAGC AAAGGCTGAT 
TGGTGGACTA ATTCGGCACA TTACAACAGA GAACGTGTAA GACGAGGAGC GACAGTAGAC 
AAAACTGTGT GCAAGAAGAA CTTGGGTAGA TTGACAAGAT TATACCTGAA ATCAGAGCAA 
GAAAGACAAC ACAATTACTT GAAGGACGGA CCATATATCT CTGCAGAAGA AGCTGTTGCA 
ATTTACACGA CTACAGTACA TTGGCTGGAA TCAAGAAGAT TTTCTCCGAT TCCTTTCCCA 
CCACTTTCTT ACAAACACGA CACAAAACTT CTTATTTTGG CTCTTGAAAG ACTGAAGGAA 
AGTTACAGTG TGAAGAATAG ATTGAATCAA TCCCAAAGAG AAGAACTGGC TTTAATTGAA 
CAGGCTTATG ATAATCCTCA CGAAGCTTTG TCTCGTATCA AGAGACACAT GCTCACGCAG 
CGTGCATTCA AAGAAGTCGG AATTGAGTTT ATGGATCTCT ACACTCATCT TATTCCAGTC 
TACGATATCG AGCCTCTTGA GAAAGTCACT GACGCTTACC TCGATCAATA CCTCTGGTAT 
GAAGCAGACA AGAGAAGATT GTTCCCGGCA TGGGTGAAAC CAGGAGATAC TGAACCACCA 
CCGCTTCTTA CATACAAATG GTGTCAAGGT CTCAACAATC TTCAAGATGT TTGGGAGACA 
AGTGAAGGAG AGTGTAATGT CATCATGGAA ACAAAATTGG AGAAAATTGC TGAAAAAATG 
GATTTGACAC TTCTCAATAG ATTGCTCAGA TTGATTGTGG ATCACAATAT TGCCGATTAC 
ATGACTTCGA AGAACAACGT TCTCATCAAC TATAAGGATA TGAACCACAC CAATTCTTTC 
GGAATTATTC GTGGTCTACA ATTTGCTTCG TTTATTGTTC AATTCTATGG ATTAGTTCTT 
GATCTTCTTG TTCTTGGACT TCGACGAGCT TCCGAAATTG CCGGTCCGCC CCAATGTCCA 
AACGAGTTCT TGCAATTCCA GGATGTTGCC ACAGAAATTG GTCACCCTAT TCGTCTCTAC 
TGCCGGTACA TCGACCGAGT TTGGATCATG TTCCGTTTCA GTGCTGATGA AGCACGTGAT 
TTGATTCAAA GATACTTGAC AGAGCATCCA GATCCGAATA ATGAGAATAT TGTCGGTTAT 
AACAACAAAA AGTGTTGGCC AAGAGACGCT AGAATGAGAT TGATGAAACA TGATGTCAAT 
TTGGGTCGTG CTGTCTTCTG GGATATCAAG AACCGTCTCC CACGTTCCAT CACAACTGTC 
GAGTGGGAAA ATTCATTCGT ATCAGTGTAC AGTAAGGATA ACCCGAACAT GTTGTTCGAC 
ATGTCCGGAT TCGAGTGTCG TATTCTACCA AAGTGTCGTA CGGCTAATGA AGAGTTTGTT 
CATCGTGATG GTGTATGGAA TCTTCAAAAC GAAGTGACAA AGGAAAGAAC TGCTCAGTGC 
TTCTTGAAGG TCGATGAGGA GTCATTGTCC AAATTCCATA ACAGAATTCG TCAAATTTTG 
ATGTCTTCTG GATCTACAAC GTTCACCAAA ATTGTCAACA AATGGAATAC AGCATTGATC 
GGATTGATGA CCTACTTCAG AGAAGCTGTT GTGAACACTC AAGAACTTTT GGATTTATTG 
GTGAAATGTG AGAATAAAAT TCAAACGAGA ATCAAGATTG GACTCAACTC AAAAATGCCC 
TCAAGATTCC CTCCAGTTGT TTTCTATACG CCAAAAGAAA TCGGAGGACT TGGAATGCTA 
TCTATGGGAC ACGTCTTGAT CCCACAGTCT GATCTTCGAT GGATGCAACA AACGGAAGCT 
GGAGGTGTCA CTCACTTCCG TTCCGGAATG AGTCACGATG AAGACCAATT GATTCCAAAT 
CTGTACAGAT ATATTCAACC TTGGGAAGCA GAGTTTGTCG ATTCTGTTCG TGTGTGGGCA 
GAATATGCGC TCAAGAGACA AGAAGCCAAC GCTCAAAATA GAAGACTCAC TTTGGAAGAT 
CTCGACGATT CATGGGATCG TGGAATTCCG AGAATCAATA CTTTGTTCCA AAAAGATCGT 
CACACATTGG CATACGATAA AGGATGGCGT GTTAGAACCG AATTCAAGGC TTATCAAATT 
CTGAAGCAAA ATCCATTCTG GTGGACACAT CAAAGACACG ACGGAAAGTT ATGGAACTTG 
AACAACTATA GAACAGACAT GATTCAAGCA CTTGGAGGAG TAGAAGGAAT TTTGGAACAT 
ACATTATTCA GGGGAACCTA CTTCCCAACA TGGGAAGGTT TGTTCTGGGA ACGTGCATCT 
GGATTCGAAG AGTCGATGAA ATTCAAGAAA CTGACAAACG CCCAACGTTC TGGACTTAAT 
CAAATTCCGA ATCGTCGTTT CACTCTCTGG TGGTCTCCAA CTATCAACCG TGCTAATGTC 
TACGTCGGTT TCCAAGTACA ACTCGATTTG ACTGGAATTT TCATGCACGG AAAGATCCCG 
ACGCTCAAGA TTTCTCTCAT TCAAATCTTC CGTGCTCACT TGTGGCAAAA GATTCACGAG 
TCAGTAGTTA TGGATCTGTG TCAAGTTTTC GATCAGGAAC TCGATGCACT TGAGATTCAA 
ACAGTCCAAA AAGAAACTAT CCATCCGAGA AAGTCTTACA AAATGAACAG TTCGTGTGCC 
GACGTACTTC TCTTTGCTCA ATACAAGTGG AATGTGTCTA GGCCTTCTTT GATGGCAGAC 
AGCAAAGATG TCATGGATAA CACCACAACT CAGAAATATT GGCTCGATGT GCAGCTTCGT 
TGGGGAGATT ATGATTCTCA CGACGTTGAA CGATACGCCC GTGCAAAATT CCTTGACTAT 
ACCACTGATA ACATGTCGAT TTATCCTTCA CCGACTGGAG TTCTCATCGC AATCGACCTC 
GCGTACAATC TATATTCTGC GTACGGAAAC TGGTTCCCAG GAATGAAACC ATTGATTCGA 
CAGGCGATGG CAAAGATCAT CAAAGCCAAT CCAGCCTTCT ATGTGCTGCG TGAACGTATT 
CGCAAGGGTC TTCAACTCTA CTCGTCGGAA CCAACTGAAC CTTATTTGAC AAGTCAAAAC 
TACGGAGAGT TGTTCAGTAA CCAAATTATC TGGTTCGTTG ATGATACCAA TGTCTACCGT 
GTCACCATTC ACAAGACATT CGAAGGTAAC CTAACGACCA AGCCAATTAA CGGAGCCATC 
TTCATCTTCA ATCCACGTAC TGGTCAGCTT TTCCTCAAGA TCATTCATAC ATCCGTATGG 
GCTGGACAGA AGCGTTTGTC GCAGTTGGCC AAGTGGAAAA CAGCAGAAGA AGTTGCTGCC 
CTCATTCGTT CGCTTCCAGT TGAAGAACAA CCACGCCAGA TCATTGTAAC AAGAAAAGCT 
ATGCTCGATC CATTGGAGGT GCATCTTCTC GATTTCCCGA ATATTGTCAT CAAGGGATCT 
GAGCTCATGT TACCATTCCA AGCTATCATG AAGGTTGAAA AATTCGGTGA TTTAATTCTC 
AAGGCCACCG AGCCACAGAT GGTGTTGTTC AATCTGTACG ATGATTGGCT CAAGACAATT 
TCTTCATACA CTGCTTTCTC TCGTGTCGTT CTTATCATGC GTGGAATGCA CATTAATCCA 
GATAAGACTA AAGTTATCTT GAAACCAGAC AAGACTACTA TCACCGAGCC TCATCATATT 
TGGCCCACAC TTTCTGACGA CGATTGGATC AAGGTTGAAT TGGCTCTGAA GGATATGATT 
CTGGCAGATT ATGGAAAGAA GAACAATGTG AATGTTGCTT CATTGACTCA ATCTGAAGTC 
AGAGATATTA TTCTTGGAAT GGAAATCTCT GCGCCAAGCC AACAACGTCA ACAGATTGCG 
GATATTGAGA AACAAACGAA AGAGCAGAGT CAAGTTACTG CAACGACTAC ACGAACTGTC 
AATAAACATG GAGATGAAAT TATCACAGCA ACTACATCGA ATTACGAAAC AGCATCGTTT 
GCTAGTCGTA CAGAATGGAG AGTTCGTGCT ATTTCATCCA CAAATCTTCA TCTTCGAACT 
CAACACATCT ATGTCAATTC CGATGATGTC AAGGATACAG GATATACTTA TATTCTTCCA 
AAGAATATCC TCAAGAAGTT CATCACTATC TCGGATCTTC GAACACAGAT TGCTGGATTC 
ATGTATGGTG TATCGCCACC TGATAATCCA CAAGTAAAGG AGATTCGGTG CATTGTCCTC 
GTTCCACAGA CTGGAAGTCA TCAGCAAGTC AATCTTCCAA CACAACTTCC GGATCATGAG 
CTTCTTCGCG ATTTTGAGCC ACTTGGATGG ATGCACACTC AACCAAATGA GTTGCCACAA 
TTGTCTCCAC AAGATGTCAC AACTCATGCC AAGCTATTGA CCGATAACAT CAGCTGGGAT 
GGAGAAAAGA CAGTGATGAT CACGTGCAGT TTCACTCCTG GATCTGTTTC TCTGACTGCA 
TATAAACTTA CACCATCGGG ATATGAATGG GGTAAAGCGA ATACCGACAA AGGAAATAAT 
CCAAAGGGAT ATATGCCAAC TCACTATGAG AAAGTGCAAA TGCTTCTATC TGATCGATTC 
CTTGGATACT TCATGGTTCC ATCAAACGGA GTGTGGAACT ACAATTTCCA AGGTCAACGT 
TGGTCGCCTG CAATGAAGTT CGATGTGTGT CTTTCTAATC CAAAGGAGTA CTATCATGAA 
GATCATCGGC CGGTTCACTT CCATAACTTT AAGGCATTCG ACGATCCACT TGGAACAGGA 
AGCGCCGATC GCGAGGATGC ATTTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498785.1
CDS	1..6990
Translation	MANYGGHPQTEPHAIPDSILEEKSRKWKQLQGKRYSEKKKFGMSDTQKEEMPPEHVRKVIRDHGDMTSRKYRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVKVLYHITGAITFVNDIPRVIEPVYMAQWGTMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEPIQMELDPEEDGAVAEWFYDHKPLATTRFVNGPTYRKWAFSIPQMSTLYRLANQLLTDLVDDNYFYLFDMKSFFTAKALNVAIPGGPKFEPLVKDLHTDEDWNEFNDINKVIIRAPIRTEYRIAFPFMYNNLISSLPVQVSWYHTPSVVFIKTEDPDLPAFYYDPLINPIVLSNLKATEENLPEGEEEDEWELPEDVRPIFEDVPLYTDNTANGLALLWAPRPFNLRSGRTRRAVDVPLVKSWYREHCPAGMPVKVRVSYQKLLKVFVLNALKHRPPKPQKRRYLFRSFKATKFFQTTTLDWVEAGLQVLRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREILRLTKLVVDAHVQYRLNNVDAYQLADGLQYIFAHVGQLTGMYRYKYKLMRQVRMCKDLKHLIYYRFNTGPVGKGPGCGFWAPGWRVWLFFLRGITPLLERWLGNLLSRQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPDGIKQNKARVILQHLSEAWRCWKANIPWKVPGLPTPVENMILRYVKAKADWWTNSAHYNRERVRRGATVDKTVCKKNLGRLTRLYLKSEQERQHNYLKDGPYISAEEAVAIYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKESYSVKNRLNQSQREELALIEQAYDNPHEALSRIKRHMLTQRAFKEVGIEFMDLYTHLIPVYDIEPLEKVTDAYLDQYLWYEADKRRLFPAWVKPGDTEPPPLLTYKWCQGLNNLQDVWETSEGECNVIMETKLEKIAEKMDLTLLNRLLRLIVDHNIADYMTSKNNVLINYKDMNHTNSFGIIRGLQFASFIVQFYGLVLDLLVLGLRRASEIAGPPQCPNEFLQFQDVATEIGHPIRLYCRYIDRVWIMFRFSADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSITTVEWENSFVSVYSKDNPNMLFDMSGFECRILPKCRTANEEFVHRDGVWNLQNEVTKERTAQCFLKVDEESLSKFHNRIRQILMSSGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKEIGGLGMLSMGHVLIPQSDLRWMQQTEAGGVTHFRSGMSHDEDQLIPNLYRYIQPWEAEFVDSVRVWAEYALKRQEANAQNRRLTLEDLDDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTEFKAYQILKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFRGTYFPTWEGLFWERASGFEESMKFKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESVVMDLCQVFDQELDALEIQTVQKETIHPRKSYKMNSSCADVLLFAQYKWNVSRPSLMADSKDVMDNTTTQKYWLDVQLRWGDYDSHDVERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLYSAYGNWFPGMKPLIRQAMAKIIKANPAFYVLRERIRKGLQLYSSEPTEPYLTSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLSQLAKWKTAEEVAALIRSLPVEEQPRQIIVTRKAMLDPLEVHLLDFPNIVIKGSELMLPFQAIMKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRVVLIMRGMHINPDKTKVILKPDKTTITEPHHIWPTLSDDDWIKVELALKDMILADYGKKNNVNVASLTQSEVRDIILGMEISAPSQQRQQIADIEKQTKEQSQVTATTTRTVNKHGDEIITATTSNYETASFASRTEWRVRAISSTNLHLRTQHIYVNSDDVKDTGYTYILPKNILKKFITISDLRTQIAGFMYGVSPPDNPQVKEIRCIVLVPQTGSHQQVNLPTQLPDHELLRDFEPLGWMHTQPNELPQLSPQDVTTHAKLLTDNISWDGEKTVMITCSFTPGSVSLTAYKLTPSGYEWGKANTDKGNNPKGYMPTHYEKVQMLLSDRFLGYFMVPSNGVWNYNFQGQRWSPAMKFDVCLSNPKEYYHEDHRPVHFHNFKAFDDPLGTGSADREDAFA

Target ORF information:

RefSeq Version	NM_066384.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Pre-mRNA-splicing factor 8 homolog (prp-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_066384.4

ORF Insert Sequence:

ATGGCCAACT ACGGCGGTCA TCCCCAAACT GAACCACATG CTATCCCGGA TTCAATTCTG 
GAAGAAAAGT CTCGAAAATG GAAACAACTT CAAGGAAAGC GGTATTCGGA GAAAAAGAAG 
TTTGGAATGT CCGATACGCA GAAAGAAGAA ATGCCACCTG AGCACGTCCG AAAAGTTATC 
AGAGATCATG GTGATATGAC TTCTCGCAAA TATCGTCATG ATAAAAGAGT CTACCTCGGT 
GCTTTGAAAT ATATGCCACA TGCCGTTTTG AAGCTACTGG AGAACATGCC GATGCCCTGG 
GAACAAATCA GAGATGTAAA AGTTCTGTAT CACATCACCG GTGCGATCAC TTTTGTCAAC 
GACATTCCGC GTGTCATCGA GCCGGTCTAC ATGGCACAAT GGGGAACGAT GTGGATTATG 
ATGAGAAGAG AAAAACGTGA TCGTCGTCAT TTCAAGCGAA TGAGATTCCC TCCATTTGAC 
GATGAAGAAC CGCCTCTCGA TTATGCTGAT AATATTCTGG ATGTCGAGCC TTTAGAGCCA 
ATTCAAATGG AACTAGATCC AGAAGAAGAC GGAGCTGTTG CTGAATGGTT CTACGATCAC 
AAGCCTCTTG CAACAACTCG ATTTGTGAAC GGCCCAACGT ACAGAAAATG GGCATTCAGT 
ATTCCACAGA TGTCCACATT GTATCGTCTT GCAAATCAGC TGCTTACCGA TTTAGTAGAT 
GATAACTACT TCTACTTGTT TGACATGAAA AGTTTCTTCA CTGCTAAAGC ACTCAACGTT 
GCAATTCCAG GAGGCCCGAA GTTCGAACCA TTGGTAAAAG ATTTACATAC TGATGAAGAC 
TGGAACGAGT TCAATGATAT CAATAAAGTT ATTATTCGAG CTCCAATTCG TACCGAGTAC 
AGAATTGCAT TCCCATTCAT GTACAACAAT CTGATCAGCT CGCTGCCTGT TCAAGTTTCA 
TGGTATCATA CTCCTTCAGT TGTATTTATC AAAACTGAAG ACCCAGATCT TCCCGCATTC 
TACTATGATC CATTGATCAA TCCAATCGTT CTCAGCAACT TGAAAGCAAC TGAAGAAAAT 
CTACCAGAAG GTGAAGAGGA AGACGAGTGG GAGCTGCCTG AAGATGTTCG ACCCATTTTT 
GAAGACGTTC CACTCTACAC CGATAACACG GCTAATGGTT TAGCACTTCT ATGGGCCCCT 
CGGCCATTCA ATCTTCGATC GGGAAGAACG AGAAGAGCAG TGGATGTACC ACTTGTCAAA 
TCGTGGTATA GAGAACATTG TCCAGCAGGA ATGCCCGTGA AAGTTCGTGT TTCTTATCAG 
AAACTGCTCA AGGTGTTTGT TCTGAACGCT CTCAAACATC GTCCACCGAA GCCCCAGAAG 
CGTCGTTATC TGTTCCGTTC CTTCAAGGCA ACAAAATTCT TCCAAACAAC CACCCTGGAT 
TGGGTAGAAG CTGGATTGCA AGTTCTTCGT CAAGGATACA ACATGCTCAA TTTGCTCATT 
CACCGAAAGA ATCTCAACTA TCTTCATCTT GATTACAACT TCAATTTGAA ACCAGTTAAA 
ACATTGACAA CAAAGGAAAG AAAGAAATCT CGTTTCGGAA ATGCATTCCA TTTGTGCAGA 
GAAATCCTTC GTCTCACAAA ACTTGTTGTT GATGCCCATG TTCAGTATCG TTTGAACAAC 
GTCGATGCGT ACCAACTTGC AGATGGTCTA CAATATATCT TTGCTCACGT CGGTCAACTG 
ACCGGAATGT ATCGTTACAA GTACAAGCTC ATGAGACAAG TCAGAATGTG CAAGGATCTC 
AAGCATCTCA TCTACTATCG TTTCAACACT GGGCCGGTTG GTAAAGGACC TGGATGTGGT 
TTTTGGGCTC CGGGATGGAG AGTTTGGTTG TTCTTCTTGC GTGGAATCAC ACCACTGCTC 
GAGAGATGGC TTGGAAATCT GTTGTCTCGA CAATTCGAAG GAAGACATTC AAAGGGAGTG 
GCCAAGACAG TCACCAAGCA AAGAGTTGAA TCTCATTTCG ATCTTGAATT ACGTGCTGCT 
GTCATGCATG ACATTCTGGA TATGATGCCG GACGGAATCA AACAAAATAA GGCTCGTGTT 
ATTCTTCAGC ATTTGTCAGA GGCGTGGAGA TGTTGGAAGG CTAATATTCC ATGGAAGGTT 
CCCGGACTGC CAACTCCAGT TGAAAATATG ATTCTACGTT ATGTGAAAGC AAAGGCTGAT 
TGGTGGACTA ATTCGGCACA TTACAACAGA GAACGTGTAA GACGAGGAGC GACAGTAGAC 
AAAACTGTGT GCAAGAAGAA CTTGGGTAGA TTGACAAGAT TATACCTGAA ATCAGAGCAA 
GAAAGACAAC ACAATTACTT GAAGGACGGA CCATATATCT CTGCAGAAGA AGCTGTTGCA 
ATTTACACGA CTACAGTACA TTGGCTGGAA TCAAGAAGAT TTTCTCCGAT TCCTTTCCCA 
CCACTTTCTT ACAAACACGA CACAAAACTT CTTATTTTGG CTCTTGAAAG ACTGAAGGAA 
AGTTACAGTG TGAAGAATAG ATTGAATCAA TCCCAAAGAG AAGAACTGGC TTTAATTGAA 
CAGGCTTATG ATAATCCTCA CGAAGCTTTG TCTCGTATCA AGAGACACAT GCTCACGCAG 
CGTGCATTCA AAGAAGTCGG AATTGAGTTT ATGGATCTCT ACACTCATCT TATTCCAGTC 
TACGATATCG AGCCTCTTGA GAAAGTCACT GACGCTTACC TCGATCAATA CCTCTGGTAT 
GAAGCAGACA AGAGAAGATT GTTCCCGGCA TGGGTGAAAC CAGGAGATAC TGAACCACCA 
CCGCTTCTTA CATACAAATG GTGTCAAGGT CTCAACAATC TTCAAGATGT TTGGGAGACA 
AGTGAAGGAG AGTGTAATGT CATCATGGAA ACAAAATTGG AGAAAATTGC TGAAAAAATG 
GATTTGACAC TTCTCAATAG ATTGCTCAGA TTGATTGTGG ATCACAATAT TGCCGATTAC 
ATGACTTCGA AGAACAACGT TCTCATCAAC TATAAGGATA TGAACCACAC CAATTCTTTC 
GGAATTATTC GTGGTCTACA ATTTGCTTCG TTTATTGTTC AATTCTATGG ATTAGTTCTT 
GATCTTCTTG TTCTTGGACT TCGACGAGCT TCCGAAATTG CCGGTCCGCC CCAATGTCCA 
AACGAGTTCT TGCAATTCCA GGATGTTGCC ACAGAAATTG GTCACCCTAT TCGTCTCTAC 
TGCCGGTACA TCGACCGAGT TTGGATCATG TTCCGTTTCA GTGCTGATGA AGCACGTGAT 
TTGATTCAAA GATACTTGAC AGAGCATCCA GATCCGAATA ATGAGAATAT TGTCGGTTAT 
AACAACAAAA AGTGTTGGCC AAGAGACGCT AGAATGAGAT TGATGAAACA TGATGTCAAT 
TTGGGTCGTG CTGTCTTCTG GGATATCAAG AACCGTCTCC CACGTTCCAT CACAACTGTC 
GAGTGGGAAA ATTCATTCGT ATCAGTGTAC AGTAAGGATA ACCCGAACAT GTTGTTCGAC 
ATGTCCGGAT TCGAGTGTCG TATTCTACCA AAGTGTCGTA CGGCTAATGA AGAGTTTGTT 
CATCGTGATG GTGTATGGAA TCTTCAAAAC GAAGTGACAA AGGAAAGAAC TGCTCAGTGC 
TTCTTGAAGG TCGATGAGGA GTCATTGTCC AAATTCCATA ACAGAATTCG TCAAATTTTG 
ATGTCTTCTG GATCTACAAC GTTCACCAAA ATTGTCAACA AATGGAATAC AGCATTGATC 
GGATTGATGA CCTACTTCAG AGAAGCTGTT GTGAACACTC AAGAACTTTT GGATTTATTG 
GTGAAATGTG AGAATAAAAT TCAAACGAGA ATCAAGATTG GACTCAACTC AAAAATGCCC 
TCAAGATTCC CTCCAGTTGT TTTCTATACG CCAAAAGAAA TCGGAGGACT TGGAATGCTA 
TCTATGGGAC ACGTCTTGAT CCCACAGTCT GATCTTCGAT GGATGCAACA AACGGAAGCT 
GGAGGTGTCA CTCACTTCCG TTCCGGAATG AGTCACGATG AAGACCAATT GATTCCAAAT 
CTGTACAGAT ATATTCAACC TTGGGAAGCA GAGTTTGTCG ATTCTGTTCG TGTGTGGGCA 
GAATATGCGC TCAAGAGACA AGAAGCCAAC GCTCAAAATA GAAGACTCAC TTTGGAAGAT 
CTCGACGATT CATGGGATCG TGGAATTCCG AGAATCAATA CTTTGTTCCA AAAAGATCGT 
CACACATTGG CATACGATAA AGGATGGCGT GTTAGAACCG AATTCAAGGC TTATCAAATT 
CTGAAGCAAA ATCCATTCTG GTGGACACAT CAAAGACACG ACGGAAAGTT ATGGAACTTG 
AACAACTATA GAACAGACAT GATTCAAGCA CTTGGAGGAG TAGAAGGAAT TTTGGAACAT 
ACATTATTCA GGGGAACCTA CTTCCCAACA TGGGAAGGTT TGTTCTGGGA ACGTGCATCT 
GGATTCGAAG AGTCGATGAA ATTCAAGAAA CTGACAAACG CCCAACGTTC TGGACTTAAT 
CAAATTCCGA ATCGTCGTTT CACTCTCTGG TGGTCTCCAA CTATCAACCG TGCTAATGTC 
TACGTCGGTT TCCAAGTACA ACTCGATTTG ACTGGAATTT TCATGCACGG AAAGATCCCG 
ACGCTCAAGA TTTCTCTCAT TCAAATCTTC CGTGCTCACT TGTGGCAAAA GATTCACGAG 
TCAGTAGTTA TGGATCTGTG TCAAGTTTTC GATCAGGAAC TCGATGCACT TGAGATTCAA 
ACAGTCCAAA AAGAAACTAT CCATCCGAGA AAGTCTTACA AAATGAACAG TTCGTGTGCC 
GACGTACTTC TCTTTGCTCA ATACAAGTGG AATGTGTCTA GGCCTTCTTT GATGGCAGAC 
AGCAAAGATG TCATGGATAA CACCACAACT CAGAAATATT GGCTCGATGT GCAGCTTCGT 
TGGGGAGATT ATGATTCTCA CGACGTTGAA CGATACGCCC GTGCAAAATT CCTTGACTAT 
ACCACTGATA ACATGTCGAT TTATCCTTCA CCGACTGGAG TTCTCATCGC AATCGACCTC 
GCGTACAATC TATATTCTGC GTACGGAAAC TGGTTCCCAG GAATGAAACC ATTGATTCGA 
CAGGCGATGG CAAAGATCAT CAAAGCCAAT CCAGCCTTCT ATGTGCTGCG TGAACGTATT 
CGCAAGGGTC TTCAACTCTA CTCGTCGGAA CCAACTGAAC CTTATTTGAC AAGTCAAAAC 
TACGGAGAGT TGTTCAGTAA CCAAATTATC TGGTTCGTTG ATGATACCAA TGTCTACCGT 
GTCACCATTC ACAAGACATT CGAAGGTAAC CTAACGACCA AGCCAATTAA CGGAGCCATC 
TTCATCTTCA ATCCACGTAC TGGTCAGCTT TTCCTCAAGA TCATTCATAC ATCCGTATGG 
GCTGGACAGA AGCGTTTGTC GCAGTTGGCC AAGTGGAAAA CAGCAGAAGA AGTTGCTGCC 
CTCATTCGTT CGCTTCCAGT TGAAGAACAA CCACGCCAGA TCATTGTAAC AAGAAAAGCT 
ATGCTCGATC CATTGGAGGT GCATCTTCTC GATTTCCCGA ATATTGTCAT CAAGGGATCT 
GAGCTCATGT TACCATTCCA AGCTATCATG AAGGTTGAAA AATTCGGTGA TTTAATTCTC 
AAGGCCACCG AGCCACAGAT GGTGTTGTTC AATCTGTACG ATGATTGGCT CAAGACAATT 
TCTTCATACA CTGCTTTCTC TCGTGTCGTT CTTATCATGC GTGGAATGCA CATTAATCCA 
GATAAGACTA AAGTTATCTT GAAACCAGAC AAGACTACTA TCACCGAGCC TCATCATATT 
TGGCCCACAC TTTCTGACGA CGATTGGATC AAGGTTGAAT TGGCTCTGAA GGATATGATT 
CTGGCAGATT ATGGAAAGAA GAACAATGTG AATGTTGCTT CATTGACTCA ATCTGAAGTC 
AGAGATATTA TTCTTGGAAT GGAAATCTCT GCGCCAAGCC AACAACGTCA ACAGATTGCG 
GATATTGAGA AACAAACGAA AGAGCAGAGT CAAGTTACTG CAACGACTAC ACGAACTGTC 
AATAAACATG GAGATGAAAT TATCACAGCA ACTACATCGA ATTACGAAAC AGCATCGTTT 
GCTAGTCGTA CAGAATGGAG AGTTCGTGCT ATTTCATCCA CAAATCTTCA TCTTCGAACT 
CAACACATCT ATGTCAATTC CGATGATGTC AAGGATACAG GATATACTTA TATTCTTCCA 
AAGAATATCC TCAAGAAGTT CATCACTATC TCGGATCTTC GAACACAGAT TGCTGGATTC 
ATGTATGGTG TATCGCCACC TGATAATCCA CAAGTAAAGG AGATTCGGTG CATTGTCCTC 
GTTCCACAGA CTGGAAGTCA TCAGCAAGTC AATCTTCCAA CACAACTTCC GGATCATGAG 
CTTCTTCGCG ATTTTGAGCC ACTTGGATGG ATGCACACTC AACCAAATGA GTTGCCACAA 
TTGTCTCCAC AAGATGTCAC AACTCATGCC AAGCTATTGA CCGATAACAT CAGCTGGGAT 
GGAGAAAAGA CAGTGATGAT CACGTGCAGT TTCACTCCTG GATCTGTTTC TCTGACTGCA 
TATAAACTTA CACCATCGGG ATATGAATGG GGTAAAGCGA ATACCGACAA AGGAAATAAT 
CCAAAGGGAT ATATGCCAAC TCACTATGAG AAAGTGCAAA TGCTTCTATC TGATCGATTC 
CTTGGATACT TCATGGTTCC ATCAAACGGA GTGTGGAACT ACAATTTCCA AGGTCAACGT 
TGGTCGCCTG CAATGAAGTT CGATGTGTGT CTTTCTAATC CAAAGGAGTA CTATCATGAA 
GATCATCGGC CGGTTCACTT CCATAACTTT AAGGCATTCG ACGATCCACT TGGAACAGGA 
AGCGCCGATC GCGAGGATGC ATTTGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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prp-8 cDNA ORF clone, Caenorhabditis elegans

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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