spp42 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	spp42
Entrez Gene ID	2543413
Full Name	U5 snRNP complex subunit Spp42
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001019290.2	NP_593861.1	U5 snRNP complex subunit Spp42

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prpf8	NP_001178519.1
Neurospora crassa	NCU07832	XP_962953.1
Arabidopsis thaliana (thale cress)	SUS2	NP_178124.2
	PRPF8	XP_001117328.1
Canis lupus familiaris (dog)	PRPF8	XP_854384.1
Drosophila melanogaster (fruit fly)	Prp8	NP_610735.1
Caenorhabditis elegans (roundworm)	prp-8	NP_498785.1
Schizosaccharomyces pombe (fission yeast)	spp42	NP_593861.1
Magnaporthe oryzae (rice blast fungus)	MGG_05244	XP_003712798.1
Oryza sativa (rice)	Os06g0167000	NP_001056917.1
Homo sapiens (human)	PRPF8	NP_006436.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006885	XP_308873.2
Kluyveromyces lactis	KLLA0A05280g	XP_451233.1
Eremothecium gossypii	AGOS_ABR207W	NP_983156.2
Bos taurus (cattle)	PRPF8	NP_001099108.1
Mus musculus (house mouse)	Prpf8	NP_619600.2
Arabidopsis thaliana (thale cress)	AT4G38780	NP_195589.2
Xenopus tropicalis (tropical clawed frog)	prpf8	XP_002936105.1
Gallus gallus (chicken)	PRPF8	XP_415805.2
Danio rerio (zebrafish)	prpf8	NP_957270.2
Saccharomyces cerevisiae (baker's yeast)	PRP8	NP_012035.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following spp42 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spp42 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102281	NM_001019290.2 Latest version!	Schizosaccharomyces pombe U5 snRNP complex subunit Spp42 (spp42), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spp42 ( NM_001019290.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593861.1 Schizosaccharomyces pombe U5 snRNP complex subunit Spp42 (spp42), mRNA.

Price & Availability↑

CloneID	OSc102281
Clone ID Related Accession (Same CDS sequence)	NM_001019290.2
Accession Version	NM_001019290.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	U5 snRNP complex subunit Spp42
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019290.1.

ATGGCGTCGT TACCACCGGG GAATCCTCCT CCACCGCCTC CTCCGCCTGG ATTTGAGCCT 
CCTTCACAGC CTCCACCTCC TCCACCTCCA GGTTATGTGA AGAAAAGAAA AAACAAGACT 
CCAGCACAGT CTGGAAATCT TGAAAAGCAA CTTAACGAAA GAGCACGCAA ATGGCGCGCT 
TCTCAAAAAT CAAAGTTTGG TGTCAAAAGG AAGCAGGGTT ATGTTCAAAC AGAGAAAGCT 
GATCTTCCGC CTGAGCACCT GCGCAAAATT ATGAAAGACA GGGGAGATAT GTCTTCTAGG 
AAGTTTCGTG CTGATAAACG TTCCTATTTG GGCGCTTTGA AGTACTTGCC ACATGCTGTG 
CTAAAATTGC TTGAGAACAT GCCAATGCCT TGGGAAGAAT ACAGGGAGGT CAAGGTTTTA 
TATCATGTAA CCGGTGCTAT AACTTTTGTA AACGAGAGTC CTCGCGTAAT TGAACCGCAT 
TTTATTGCTC AATGGGGAAC GATGTGGATG ATGATGAGAC GCGAGAAGCG TGATCGAAAA 
AATTTTAAGC GTTTGCGGTT TCCTCCTTTT GACGATGAAG AGCCACCATT TTCTATTGAT 
CAATTGCTTG ATTTAGAGCC GTTAGAGGCT ATTAGAATGG ACTTAGATGA AGAAGATGAC 
GCTCCTGTTA TGGATTGGTT TTACGAAAAT AAGGCTTTGG AAGATACTCC CCATGTAAAT 
GGTCCAACCT ATCGTCGTTG GAAGCTTAAT TTACCTCAGA TGGCTAATTT ACATCGTTTA 
GGTTACCAAC TTTTGAGTGA TTTACGCGAT GATAATTACT TTTACTTGTT TAATGACAAT 
AGTTTCTTTA CGGCTAAAGC TTTGAACGTG GCTATCCCAG GAGGTCCGAA ATTTGAACCT 
TTATACAAAG ACGAAGCCCC AGAAATGGAA GATTGGAATG AATTTAATGA CATTTATAAA 
CTTATTATCC GACATCCCAT TAAAACTGAA TACCGGATAG CGTTTCCTTA CCTTTATAAT 
TCTAGAGCTC GAAGCGTTGC ACTAAGTGAA TACCACCAAC CGTCTAATGT TTTCGTTCCT 
CCTGAGGATC CTGATCTTCC GGCTTTTTTT TGGGATCCCA TAATAAACCC CATCACTTCC 
CGCCAACTCA CTTTGCATGA ACTAGATACC TCTCCCGAAG ATTCTGCCAT AGAAGAAGAT 
CCTAATTTTG AAATCCCTTT CGATCCTTTC TTTCATTCCG AAGATATAGA ATTTGAGCAC 
ACAGCCAGTG CTCTGATTTT ATTGTGGGCA CCCCATCCTT TTAATAAGCG ATCTGGTGCG 
ACTAAGCGTG CGCAAGATGT TCCACTGATA AAGCATTGGT ATTTAGAGCA TTGTCCTCCT 
AATCAGCCTG TTAAAGTTCG TGTGTCATAT CAAAAACTTT TAAAATCCCA TGTTATGAAT 
AAGTTGCACA TGGCGCATCC CAAATCGCAC ACCAATCGCA GCTTACTTAG ACAGTTAAAA 
AATACTAAAT TTTTTCAAAG TACTTCTATC GATTGGGTTG AAGCTGGTCT CCAAGTTTGT 
CGCCAAGGGT ACAACATGCT GCAATTACTT ATTCACAGAA AAGGGTTGAC ATATTTACAT 
TTGGATTACA ACTGTAATTT AAAACCTACA AAGACATTAA CTACGAAGGA AAGAAAGAAA 
TCTAGGTTTG GTAATGCATT TCACTTGATG CGCGAAATAC TTCGCCTTAC TAAATTAATT 
GTCGATTCTC ATGTACAGTA TCGTTTGGGA AACATTGACG CATATCAGCT AGCTGATGGA 
TTACATTACA TATTTAATCA TGTTGGTCAA CTTACGGGAA TGTATCGTTA TAAATATCGA 
TTGATGCGGC AGATTCGTGC TTGCAAAGAT TTCAAGCATC TTATTTATTA TCGCTTCAAC 
ACTGGTCCTG TTGGCAAAGG TCCAGGTTGT GGGTTTTGGG CTCCCAGTTG GCGTGTATGG 
CTTTTCTTTC TACGCGGAAT TGTTCCTTTG CTAGAGCGCT GGCTGGGCAA TTTGCTTGCT 
AGACAATTTG AAGGCCGTCA TTCTACAGGA GTGGCAAAAC AAATAACCAA GCAGCGTGTC 
GATTCTCATC AAGATCTAGA ATTGAGGGCT GCAGTGATGA ATGATATTTT GGATATGATA 
CCTGAAGGAA TTCGCCAAGG CAAAAGCAAA ACCATTTTGC AGCATCTTAG TGAAGCTTGG 
CGTTGTTGGA AGGCTAACAT ACCTTGGAAG GTTCCGGGTC TCCCTGCACC TATTGAGAAT 
ATGATTTTGA GATATGTGAA GTCAAAAGCA GATTGGTGGA CCTCAGTCGC TCACTTCAAT 
CGTGAACGTA TTCGTCGAGG AGCTACTGTT GATAAAACCG TGGCGAAAAA GAATCTTGGC 
AGATTAACTA GGCTTTGGTT AAAAGCTGAA CAAGAAAGGC AACATAATTA TCTAAAAGAT 
GGACCTTATG TAACTGCCGA TGAGGCAGTT GCTATATATA CTACTTTTGT ACATTGGTTA 
GAAAGTAGGC GATTCCAACC TATTCCCTTT CCTCCACTTT CGTACAAACA TGATACGAAA 
CTTCTTGTTT TAGCACTTGA ACGCTTAAAA GAAGCGTATT CGGTGAAGGG TCGCTTGAAT 
CAATCCCAAC GAGAGGAATT AGCATTAGTG GAACAAGCCT ACGATAATCC ACATGAGATG 
CTGTCCCAAA TTAAAAGGCG TTTATTGACA ATGCGTACTT TTAAAGAGGT AGGGATTGAG 
TTTATGGATA TGTATTCTCA CTTGATTCCT GTTTATTCTG TAGATCCGAT GGAGAAAATT 
TGTGATGCTT ATCTCGATCA ATACCTTTGG TTTGAGGCCG ATCGTCGACA CCTATTTCCT 
TCCTGGGTAA AGCCTTCTGA TTCAGAACCT CCACCTCTTC TTGTTTACAA ATGGTGCCAA 
GGAATTAATA ATCTTACAGA TGTTTGGGAA ACTTCCAACG GCGAATGTAA CGTTTTAATG 
GAAACTAGGC TGTCTAAAGT ATTTGAGAAA GTTGACTTAA CACTTCTTAA TAGACTGATG 
TCATTACTAA TGGACACCAA TTTAGCTTCC TACGCTAGTG CTAAAAACAA CGTTGTTTTG 
TCATATAAGG ATATGTCGCA TACCAACTCG TATGGCCTAG TCCGTGGTTT ACAATTTTCA 
AGTTTCATCT GGCAGTTTTA TGGATTAGTG TTGGATTTAC TAATACTTGG CCTTCAACGC 
GCTACAGAAA TAGCTGGTCC TGCAGATGCA CCGAATGATT TTCTACACTT CAAAGATCAG 
GCAACTGAGA CCAGTCACCC TATTCGACTG TATACACGCT ATATTGATAA AGTTTATATA 
ATGTTTCGAT TTACTGATGA AGAATCCCGT GATTTGATTC AACGATTTTT GAATGAAAAT 
CCTGATCCCA CAAACAGCAA TGTTGTAAAT TATTCTAAAG GTAAAAAAAA TTGTTGGCCT 
CGAGATGCTC GTATGAGACT CATGAAACAC GACGTGAATC TCGGACGTGC TGTCTTTTGG 
GAGATTCGTA ATCGTTTACC TAGATCACTA ACTACCCTTG AGTGGGAGGA TACCTTTCCT 
AGTGTTTACA GCAAAGACAA TCCTAATTTA TTGTTCTCGA TGACAGGATT CGAGGTTAGA 
ATCCTTCCAA AGATCCGACA AAATGAAGAA TTTTCTTTGA AGGATGGTGT ATGGAATCTC 
ACAGATAATC GTACGAAACA ACGGACAGCG CAAGCTTTCA TACGTGTTAC GGAGGATGGT 
ATAAATCAAT TTGGAAACCG TATACGTCAG ATTCTTATGT CCTCTGGTTC CACTACCTTT 
ACCAAGATTG CTAATAAATG GAATACTGCT CTAATAGCTC TCATGACTTA TTATAGGGAA 
GCCGCGATTT CTACGCCCGA GCTTCTAGAT TTACTTGTTA AATGTGAATC GAAAATACAA 
ACTCGAGTAA AAATTTCATT AAATTCTAAA ATGCCTAGTC GCTTTCCTCC AGCCGTTTTC 
TACTCGCCTA AAGAACTTGG TGGATTAGGA ATGCTTTCAA TGGGTCATGT ACTTATTCCA 
CAATCTGATC TTAGATGGTC GAAACAAACT GATACTGGTA TTACTCATTT TCGAAGTGGA 
ATGACTACCA ATGGAGAACA TTTGATTCCA AACCTTTATC GTTATATTCA GCCATGGGAA 
TCAGAGTTTA TTGACTCTCA GCGTGTATGG GCTGAGTATG CAATGAAGAG ACAAGAAGCT 
CTACAACAAA ATCGTCGTTT AACTTTAGAG GATCTGGAAG ATTCATGGGA TCGAGGAATT 
CCTCGTATAA ATACTCTATT TCAAAAAGAT AGACACACAT TGGCATATGA TAAAGGATGG 
CGTGTTAGGA CTGAGTTTAA GCAGTACCAG CTCCTTAAAA ACAATCCATT CTGGTGGACG 
AGTCAAAGAC ATGATGGAAA ATTATGGCAA TTAAATAATT ACCGAGTAGA TGTCATTCAG 
GCTTTAGGTG GTGTCGAAGG TATTTTGGAG CATACGATGT TTAAAGCTAC TGGATTTCCT 
TCATGGGAAG GTCTTTTTTG GGAAAAAGCA TCTGGTTTTG AAGAATCCAT GAAGTTCAAA 
AAGCTTACTA ATGCTCAGCG TTCAGGTTTA AATCAAATCC CTAACCGTAG ATTTACGCTT 
TGGTGGAGTC CCACTATCAA TCGTGCTAAC GTTTATGTCG GATTTCAAGT ACAACTGGAT 
TTGACTGGAA TCATGATGCA TGGCAAAATT CCAACCCTGA AGATTTCACT CATTCAAATT 
TTTCGTTCTC ATTTATGGCA AAAAATTCAT GAATCTGTTG TTTGGGATTT GTGTCAAGTG 
CTTGACCAAG AATTAGAGTC ATTGCAAATT GAAACAGTCC AAAAAGAAAC TATCCATCCT 
CGTAAAAGTT ATAAAATGAA TAGTTCTTGT GCAGATATCC TCCTTTTGGC TGCCTATAAA 
TGGAACGTAT CAAGGCCATC CTTATTGAAC GATAATCGCG ATGTGCTTGA CAACACTACC 
ACCAACAAAT ATTGGATTGA TGTACAACTT CGATTTGGAG ATTATGATTC TCATGATATT 
GAACGTTATA CTAGAGCTAA ATTTTTGGAT TATAGCACTG ATGCTCAAAG CATGTATCCT 
TCACCGACCG GTGTCTTAAT CGGCATTGAT CTTTGTTACA ATATGCATAG TGCATATGGT 
AACTGGATTC CTGGTATGAA ACCATTAATT CAACAGAGCA TGAACAAGAT CATGAAAGCC 
AATCCAGCCT TATATGTGTT GCGTGAACGC ATACGAAAAG GTCTTCAATT ATATGCTTCT 
GAACCCCAAG AGCAGTATTT GTCCTCCTCA AATTATGCTG AACTCTTTAG TAATCAAATT 
CAATTATTCG TTGATGATAC TAATGTTTAT CGGGTTACCA TTCATAAAAC ATTTGAAGGT 
AATTTGACTA CGAAACCCAT TAATGGTGCT ATTTTTATAT TCAATCCGAG AACTGGTCAA 
TTGTTCTTAA AAGTTATTCA TACGTCAGTT TGGGCGGGAC AAAAACGATT GGGACAATTG 
GCGAAATGGA AGACTGCTGA AGAAGTTGCT GCTTTGATTC GTTCTCTGCC TGTTGAAGAA 
CAACCTCGAC AGATTATAGT AACTCGAAAG GGCATGTTGG ATCCATTAGA AGTTCATCTT 
TTGGACTTTC CTAATATAAC CATTAAGGGT AGTGAATTAC AGCTTCCTTT CCAAGCGATT 
ATTAAACTTG ATAAAATCAA TGATTTGATT TTACGTGCCA CCGAACCGCA AATGGTACTT 
TTCAATTTAT ATGATGACTG GCTTCAATCG GTATCATCGT ATACAGCTTT TTCACGCTTG 
ATACTTATCT TGCGCGCATT GAATGTCAAT ACTGAGAAAA CGAAGCTAAT TCTTAGACCA 
GACAAGTCTA TAATTACAAA AGAGAATCAC GTGTGGCCCA ACCTGGATGA TCAACAATGG 
CTTGATGTTG AACCGAAACT TAGAGATTTG ATCCTTGCTG ATTATGCTAA GAAAAACAAT 
ATTAACGTTG CTAGTCTTAC GAACTCGGAA GTGCGAGATA TTATTCTCGG TATGACAATA 
ACGGCTCCTT CTCTTCAACG TCAACAGATA GCTGAAATAG AAAAGCAAGG CCGTGAGAAT 
GCCCAAGTTA CGGCTGTCAC CACAAAAACT ACTAACGTGC ATGGTGATGA AATGGTGGTT 
ACTACAACAT CTGCGTATGA AAATGAAAAG TTTTCTAGTA AAACAGAATG GAGAAATCGT 
GCCATATCAT CTATCAGTTT ACCCCTTCGT ACAAAAAACA TTTATGTTAA CTCTGATAAT 
ATCAGCGAAA CTTTTCCTTA TACATACATT CTTCCTCAAA ATCTGCTTCG TAAGTTTGTT 
ACGATATCCG ATTTAAGGAC ACAGGTTGCC GGATATATGT ATGGTAAAAG TCCGTCAGAC 
AACCCTCAAA TTAAGGAAAT TCGTTGTATT GCACTTGTAC CACAGCTTGG CAGTATAAGA 
AATGTGCAGC TTCCTTCTAA ACTGCCTCAT GATCTTCAGC CTTCTATATT AGAAGACCTT 
GAGCCCCTTG GTTGGATACA CACTCAAAGT TCTGAACTTC CCTATTTATC ATCTGTCGAT 
GTAACTACAC ATGCAAAAAT TTTATCTTCT CATCCTGAAT GGGATACCAA AGCTGTAACT 
TTGACAGTGA GCTACATTCC TGGTTCGATA TCCTTAGCTG CATATACTGT GTCAAAGGAA 
GGAATTGAGT GGGGATCAAA AAACATGGAT ATCAACAGTG ATGAAGCTAT CGGGTATGAA 
CCTTCTATGG CTGAAAAATG TCAGCTGCTA TTGTCCGATA GAATTCAAGG ATTTTTCTTG 
GTACCAGAAG AGGGCGTATG GAATTATAAT TTCAATGGTG CAAGCTTCAG TCCTAAAATG 
ACGTACAGTT TAAAACTTGA TGTCCCTCTG CCATTCTTTG CTTTAGAGCA TAGACCTACT 
CATGTAATAA GCTATACTGA ACTGGAGACG AATGACAGAT TAGAGGAAGA TATGCCAGAT 
GCATTTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_593861.1
CDS	51..7142
Translation	MASLPPGNPPPPPPPPGFEPPSQPPPPPPPGYVKKRKNKTPAQSGNLEKQLNERARKWRASQKSKFGVKRKQGYVQTEKADLPPEHLRKIMKDRGDMSSRKFRADKRSYLGALKYLPHAVLKLLENMPMPWEEYREVKVLYHVTGAITFVNESPRVIEPHFIAQWGTMWMMMRREKRDRKNFKRLRFPPFDDEEPPFSIDQLLDLEPLEAIRMDLDEEDDAPVMDWFYENKALEDTPHVNGPTYRRWKLNLPQMANLHRLGYQLLSDLRDDNYFYLFNDNSFFTAKALNVAIPGGPKFEPLYKDEAPEMEDWNEFNDIYKLIIRHPIKTEYRIAFPYLYNSRARSVALSEYHQPSNVFVPPEDPDLPAFFWDPIINPITSRQLTLHELDTSPEDSAIEEDPNFEIPFDPFFHSEDIEFEHTASALILLWAPHPFNKRSGATKRAQDVPLIKHWYLEHCPPNQPVKVRVSYQKLLKSHVMNKLHMAHPKSHTNRSLLRQLKNTKFFQSTSIDWVEAGLQVCRQGYNMLQLLIHRKGLTYLHLDYNCNLKPTKTLTTKERKKSRFGNAFHLMREILRLTKLIVDSHVQYRLGNIDAYQLADGLHYIFNHVGQLTGMYRYKYRLMRQIRACKDFKHLIYYRFNTGPVGKGPGCGFWAPSWRVWLFFLRGIVPLLERWLGNLLARQFEGRHSTGVAKQITKQRVDSHQDLELRAAVMNDILDMIPEGIRQGKSKTILQHLSEAWRCWKANIPWKVPGLPAPIENMILRYVKSKADWWTSVAHFNRERIRRGATVDKTVAKKNLGRLTRLWLKAEQERQHNYLKDGPYVTADEAVAIYTTFVHWLESRRFQPIPFPPLSYKHDTKLLVLALERLKEAYSVKGRLNQSQREELALVEQAYDNPHEMLSQIKRRLLTMRTFKEVGIEFMDMYSHLIPVYSVDPMEKICDAYLDQYLWFEADRRHLFPSWVKPSDSEPPPLLVYKWCQGINNLTDVWETSNGECNVLMETRLSKVFEKVDLTLLNRLMSLLMDTNLASYASAKNNVVLSYKDMSHTNSYGLVRGLQFSSFIWQFYGLVLDLLILGLQRATEIAGPADAPNDFLHFKDQATETSHPIRLYTRYIDKVYIMFRFTDEESRDLIQRFLNENPDPTNSNVVNYSKGKKNCWPRDARMRLMKHDVNLGRAVFWEIRNRLPRSLTTLEWEDTFPSVYSKDNPNLLFSMTGFEVRILPKIRQNEEFSLKDGVWNLTDNRTKQRTAQAFIRVTEDGINQFGNRIRQILMSSGSTTFTKIANKWNTALIALMTYYREAAISTPELLDLLVKCESKIQTRVKISLNSKMPSRFPPAVFYSPKELGGLGMLSMGHVLIPQSDLRWSKQTDTGITHFRSGMTTNGEHLIPNLYRYIQPWESEFIDSQRVWAEYAMKRQEALQQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTEFKQYQLLKNNPFWWTSQRHDGKLWQLNNYRVDVIQALGGVEGILEHTMFKATGFPSWEGLFWEKASGFEESMKFKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIMMHGKIPTLKISLIQIFRSHLWQKIHESVVWDLCQVLDQELESLQIETVQKETIHPRKSYKMNSSCADILLLAAYKWNVSRPSLLNDNRDVLDNTTTNKYWIDVQLRFGDYDSHDIERYTRAKFLDYSTDAQSMYPSPTGVLIGIDLCYNMHSAYGNWIPGMKPLIQQSMNKIMKANPALYVLRERIRKGLQLYASEPQEQYLSSSNYAELFSNQIQLFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKVIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPRQIIVTRKGMLDPLEVHLLDFPNITIKGSELQLPFQAIIKLDKINDLILRATEPQMVLFNLYDDWLQSVSSYTAFSRLILILRALNVNTEKTKLILRPDKSIITKENHVWPNLDDQQWLDVEPKLRDLILADYAKKNNINVASLTNSEVRDIILGMTITAPSLQRQQIAEIEKQGRENAQVTAVTTKTTNVHGDEMVVTTTSAYENEKFSSKTEWRNRAISSISLPLRTKNIYVNSDNISETFPYTYILPQNLLRKFVTISDLRTQVAGYMYGKSPSDNPQIKEIRCIALVPQLGSIRNVQLPSKLPHDLQPSILEDLEPLGWIHTQSSELPYLSSVDVTTHAKILSSHPEWDTKAVTLTVSYIPGSISLAAYTVSKEGIEWGSKNMDINSDEAIGYEPSMAEKCQLLLSDRIQGFFLVPEEGVWNYNFNGASFSPKMTYSLKLDVPLPFFALEHRPTHVISYTELETNDRLEEDMPDAFA

Target ORF information:

RefSeq Version	NM_001019290.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe U5 snRNP complex subunit Spp42 (spp42), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019290.2

ORF Insert Sequence:

ATGGCGTCGT TACCACCGGG GAATCCTCCT CCACCGCCTC CTCCGCCTGG ATTTGAGCCT 
CCTTCACAGC CTCCACCTCC TCCACCTCCA GGTTATGTGA AGAAAAGAAA AAACAAGACT 
CCAGCACAGT CTGGAAATCT TGAAAAGCAA CTTAACGAAA GAGCACGCAA ATGGCGCGCT 
TCTCAAAAAT CAAAGTTTGG TGTCAAAAGG AAGCAGGGTT ATGTTCAAAC AGAGAAAGCT 
GATCTTCCGC CTGAGCACCT GCGCAAAATT ATGAAAGACA GGGGAGATAT GTCTTCTAGG 
AAGTTTCGTG CTGATAAACG TTCCTATTTG GGCGCTTTGA AGTACTTGCC ACATGCTGTG 
CTAAAATTGC TTGAGAACAT GCCAATGCCT TGGGAAGAAT ACAGGGAGGT CAAGGTTTTA 
TATCATGTAA CCGGTGCTAT AACTTTTGTA AACGAGAGTC CTCGCGTAAT TGAACCGCAT 
TTTATTGCTC AATGGGGAAC GATGTGGATG ATGATGAGAC GCGAGAAGCG TGATCGAAAA 
AATTTTAAGC GTTTGCGGTT TCCTCCTTTT GACGATGAAG AGCCACCATT TTCTATTGAT 
CAATTGCTTG ATTTAGAGCC GTTAGAGGCT ATTAGAATGG ACTTAGATGA AGAAGATGAC 
GCTCCTGTTA TGGATTGGTT TTACGAAAAT AAGGCTTTGG AAGATACTCC CCATGTAAAT 
GGTCCAACCT ATCGTCGTTG GAAGCTTAAT TTACCTCAGA TGGCTAATTT ACATCGTTTA 
GGTTACCAAC TTTTGAGTGA TTTACGCGAT GATAATTACT TTTACTTGTT TAATGACAAT 
AGTTTCTTTA CGGCTAAAGC TTTGAACGTG GCTATCCCAG GAGGTCCGAA ATTTGAACCT 
TTATACAAAG ACGAAGCCCC AGAAATGGAA GATTGGAATG AATTTAATGA CATTTATAAA 
CTTATTATCC GACATCCCAT TAAAACTGAA TACCGGATAG CGTTTCCTTA CCTTTATAAT 
TCTAGAGCTC GAAGCGTTGC ACTAAGTGAA TACCACCAAC CGTCTAATGT TTTCGTTCCT 
CCTGAGGATC CTGATCTTCC GGCTTTTTTT TGGGATCCCA TAATAAACCC CATCACTTCC 
CGCCAACTCA CTTTGCATGA ACTAGATACC TCTCCCGAAG ATTCTGCCAT AGAAGAAGAT 
CCTAATTTTG AAATCCCTTT CGATCCTTTC TTTCATTCCG AAGATATAGA ATTTGAGCAC 
ACAGCCAGTG CTCTGATTTT ATTGTGGGCA CCCCATCCTT TTAATAAGCG ATCTGGTGCG 
ACTAAGCGTG CGCAAGATGT TCCACTGATA AAGCATTGGT ATTTAGAGCA TTGTCCTCCT 
AATCAGCCTG TTAAAGTTCG TGTGTCATAT CAAAAACTTT TAAAATCCCA TGTTATGAAT 
AAGTTGCACA TGGCGCATCC CAAATCGCAC ACCAATCGCA GCTTACTTAG ACAGTTAAAA 
AATACTAAAT TTTTTCAAAG TACTTCTATC GATTGGGTTG AAGCTGGTCT CCAAGTTTGT 
CGCCAAGGGT ACAACATGCT GCAATTACTT ATTCACAGAA AAGGGTTGAC ATATTTACAT 
TTGGATTACA ACTGTAATTT AAAACCTACA AAGACATTAA CTACGAAGGA AAGAAAGAAA 
TCTAGGTTTG GTAATGCATT TCACTTGATG CGCGAAATAC TTCGCCTTAC TAAATTAATT 
GTCGATTCTC ATGTACAGTA TCGTTTGGGA AACATTGACG CATATCAGCT AGCTGATGGA 
TTACATTACA TATTTAATCA TGTTGGTCAA CTTACGGGAA TGTATCGTTA TAAATATCGA 
TTGATGCGGC AGATTCGTGC TTGCAAAGAT TTCAAGCATC TTATTTATTA TCGCTTCAAC 
ACTGGTCCTG TTGGCAAAGG TCCAGGTTGT GGGTTTTGGG CTCCCAGTTG GCGTGTATGG 
CTTTTCTTTC TACGCGGAAT TGTTCCTTTG CTAGAGCGCT GGCTGGGCAA TTTGCTTGCT 
AGACAATTTG AAGGCCGTCA TTCTACAGGA GTGGCAAAAC AAATAACCAA GCAGCGTGTC 
GATTCTCATC AAGATCTAGA ATTGAGGGCT GCAGTGATGA ATGATATTTT GGATATGATA 
CCTGAAGGAA TTCGCCAAGG CAAAAGCAAA ACCATTTTGC AGCATCTTAG TGAAGCTTGG 
CGTTGTTGGA AGGCTAACAT ACCTTGGAAG GTTCCGGGTC TCCCTGCACC TATTGAGAAT 
ATGATTTTGA GATATGTGAA GTCAAAAGCA GATTGGTGGA CCTCAGTCGC TCACTTCAAT 
CGTGAACGTA TTCGTCGAGG AGCTACTGTT GATAAAACCG TGGCGAAAAA GAATCTTGGC 
AGATTAACTA GGCTTTGGTT AAAAGCTGAA CAAGAAAGGC AACATAATTA TCTAAAAGAT 
GGACCTTATG TAACTGCCGA TGAGGCAGTT GCTATATATA CTACTTTTGT ACATTGGTTA 
GAAAGTAGGC GATTCCAACC TATTCCCTTT CCTCCACTTT CGTACAAACA TGATACGAAA 
CTTCTTGTTT TAGCACTTGA ACGCTTAAAA GAAGCGTATT CGGTGAAGGG TCGCTTGAAT 
CAATCCCAAC GAGAGGAATT AGCATTAGTG GAACAAGCCT ACGATAATCC ACATGAGATG 
CTGTCCCAAA TTAAAAGGCG TTTATTGACA ATGCGTACTT TTAAAGAGGT AGGGATTGAG 
TTTATGGATA TGTATTCTCA CTTGATTCCT GTTTATTCTG TAGATCCGAT GGAGAAAATT 
TGTGATGCTT ATCTCGATCA ATACCTTTGG TTTGAGGCCG ATCGTCGACA CCTATTTCCT 
TCCTGGGTAA AGCCTTCTGA TTCAGAACCT CCACCTCTTC TTGTTTACAA ATGGTGCCAA 
GGAATTAATA ATCTTACAGA TGTTTGGGAA ACTTCCAACG GCGAATGTAA CGTTTTAATG 
GAAACTAGGC TGTCTAAAGT ATTTGAGAAA GTTGACTTAA CACTTCTTAA TAGACTGATG 
TCATTACTAA TGGACACCAA TTTAGCTTCC TACGCTAGTG CTAAAAACAA CGTTGTTTTG 
TCATATAAGG ATATGTCGCA TACCAACTCG TATGGCCTAG TCCGTGGTTT ACAATTTTCA 
AGTTTCATCT GGCAGTTTTA TGGATTAGTG TTGGATTTAC TAATACTTGG CCTTCAACGC 
GCTACAGAAA TAGCTGGTCC TGCAGATGCA CCGAATGATT TTCTACACTT CAAAGATCAG 
GCAACTGAGA CCAGTCACCC TATTCGACTG TATACACGCT ATATTGATAA AGTTTATATA 
ATGTTTCGAT TTACTGATGA AGAATCCCGT GATTTGATTC AACGATTTTT GAATGAAAAT 
CCTGATCCCA CAAACAGCAA TGTTGTAAAT TATTCTAAAG GTAAAAAAAA TTGTTGGCCT 
CGAGATGCTC GTATGAGACT CATGAAACAC GACGTGAATC TCGGACGTGC TGTCTTTTGG 
GAGATTCGTA ATCGTTTACC TAGATCACTA ACTACCCTTG AGTGGGAGGA TACCTTTCCT 
AGTGTTTACA GCAAAGACAA TCCTAATTTA TTGTTCTCGA TGACAGGATT CGAGGTTAGA 
ATCCTTCCAA AGATCCGACA AAATGAAGAA TTTTCTTTGA AGGATGGTGT ATGGAATCTC 
ACAGATAATC GTACGAAACA ACGGACAGCG CAAGCTTTCA TACGTGTTAC GGAGGATGGT 
ATAAATCAAT TTGGAAACCG TATACGTCAG ATTCTTATGT CCTCTGGTTC CACTACCTTT 
ACCAAGATTG CTAATAAATG GAATACTGCT CTAATAGCTC TCATGACTTA TTATAGGGAA 
GCCGCGATTT CTACGCCCGA GCTTCTAGAT TTACTTGTTA AATGTGAATC GAAAATACAA 
ACTCGAGTAA AAATTTCATT AAATTCTAAA ATGCCTAGTC GCTTTCCTCC AGCCGTTTTC 
TACTCGCCTA AAGAACTTGG TGGATTAGGA ATGCTTTCAA TGGGTCATGT ACTTATTCCA 
CAATCTGATC TTAGATGGTC GAAACAAACT GATACTGGTA TTACTCATTT TCGAAGTGGA 
ATGACTACCA ATGGAGAACA TTTGATTCCA AACCTTTATC GTTATATTCA GCCATGGGAA 
TCAGAGTTTA TTGACTCTCA GCGTGTATGG GCTGAGTATG CAATGAAGAG ACAAGAAGCT 
CTACAACAAA ATCGTCGTTT AACTTTAGAG GATCTGGAAG ATTCATGGGA TCGAGGAATT 
CCTCGTATAA ATACTCTATT TCAAAAAGAT AGACACACAT TGGCATATGA TAAAGGATGG 
CGTGTTAGGA CTGAGTTTAA GCAGTACCAG CTCCTTAAAA ACAATCCATT CTGGTGGACG 
AGTCAAAGAC ATGATGGAAA ATTATGGCAA TTAAATAATT ACCGAGTAGA TGTCATTCAG 
GCTTTAGGTG GTGTCGAAGG TATTTTGGAG CATACGATGT TTAAAGCTAC TGGATTTCCT 
TCATGGGAAG GTCTTTTTTG GGAAAAAGCA TCTGGTTTTG AAGAATCCAT GAAGTTCAAA 
AAGCTTACTA ATGCTCAGCG TTCAGGTTTA AATCAAATCC CTAACCGTAG ATTTACGCTT 
TGGTGGAGTC CCACTATCAA TCGTGCTAAC GTTTATGTCG GATTTCAAGT ACAACTGGAT 
TTGACTGGAA TCATGATGCA TGGCAAAATT CCAACCCTGA AGATTTCACT CATTCAAATT 
TTTCGTTCTC ATTTATGGCA AAAAATTCAT GAATCTGTTG TTTGGGATTT GTGTCAAGTG 
CTTGACCAAG AATTAGAGTC ATTGCAAATT GAAACAGTCC AAAAAGAAAC TATCCATCCT 
CGTAAAAGTT ATAAAATGAA TAGTTCTTGT GCAGATATCC TCCTTTTGGC TGCCTATAAA 
TGGAACGTAT CAAGGCCATC CTTATTGAAC GATAATCGCG ATGTGCTTGA CAACACTACC 
ACCAACAAAT ATTGGATTGA TGTACAACTT CGATTTGGAG ATTATGATTC TCATGATATT 
GAACGTTATA CTAGAGCTAA ATTTTTGGAT TATAGCACTG ATGCTCAAAG CATGTATCCT 
TCACCGACCG GTGTCTTAAT CGGCATTGAT CTTTGTTACA ATATGCATAG TGCATATGGT 
AACTGGATTC CTGGTATGAA ACCATTAATT CAACAGAGCA TGAACAAGAT CATGAAAGCC 
AATCCAGCCT TATATGTGTT GCGTGAACGC ATACGAAAAG GTCTTCAATT ATATGCTTCT 
GAACCCCAAG AGCAGTATTT GTCCTCCTCA AATTATGCTG AACTCTTTAG TAATCAAATT 
CAATTATTCG TTGATGATAC TAATGTTTAT CGGGTTACCA TTCATAAAAC ATTTGAAGGT 
AATTTGACTA CGAAACCCAT TAATGGTGCT ATTTTTATAT TCAATCCGAG AACTGGTCAA 
TTGTTCTTAA AAGTTATTCA TACGTCAGTT TGGGCGGGAC AAAAACGATT GGGACAATTG 
GCGAAATGGA AGACTGCTGA AGAAGTTGCT GCTTTGATTC GTTCTCTGCC TGTTGAAGAA 
CAACCTCGAC AGATTATAGT AACTCGAAAG GGCATGTTGG ATCCATTAGA AGTTCATCTT 
TTGGACTTTC CTAATATAAC CATTAAGGGT AGTGAATTAC AGCTTCCTTT CCAAGCGATT 
ATTAAACTTG ATAAAATCAA TGATTTGATT TTACGTGCCA CCGAACCGCA AATGGTACTT 
TTCAATTTAT ATGATGACTG GCTTCAATCG GTATCATCGT ATACAGCTTT TTCACGCTTG 
ATACTTATCT TGCGCGCATT GAATGTCAAT ACTGAGAAAA CGAAGCTAAT TCTTAGACCA 
GACAAGTCTA TAATTACAAA AGAGAATCAC GTGTGGCCCA ACCTGGATGA TCAACAATGG 
CTTGATGTTG AACCGAAACT TAGAGATTTG ATCCTTGCTG ATTATGCTAA GAAAAACAAT 
ATTAACGTTG CTAGTCTTAC GAACTCGGAA GTGCGAGATA TTATTCTCGG TATGACAATA 
ACGGCTCCTT CTCTTCAACG TCAACAGATA GCTGAAATAG AAAAGCAAGG CCGTGAGAAT 
GCCCAAGTTA CGGCTGTCAC CACAAAAACT ACTAACGTGC ATGGTGATGA AATGGTGGTT 
ACTACAACAT CTGCGTATGA AAATGAAAAG TTTTCTAGTA AAACAGAATG GAGAAATCGT 
GCCATATCAT CTATCAGTTT ACCCCTTCGT ACAAAAAACA TTTATGTTAA CTCTGATAAT 
ATCAGCGAAA CTTTTCCTTA TACATACATT CTTCCTCAAA ATCTGCTTCG TAAGTTTGTT 
ACGATATCCG ATTTAAGGAC ACAGGTTGCC GGATATATGT ATGGTAAAAG TCCGTCAGAC 
AACCCTCAAA TTAAGGAAAT TCGTTGTATT GCACTTGTAC CACAGCTTGG CAGTATAAGA 
AATGTGCAGC TTCCTTCTAA ACTGCCTCAT GATCTTCAGC CTTCTATATT AGAAGACCTT 
GAGCCCCTTG GTTGGATACA CACTCAAAGT TCTGAACTTC CCTATTTATC ATCTGTCGAT 
GTAACTACAC ATGCAAAAAT TTTATCTTCT CATCCTGAAT GGGATACCAA AGCTGTAACT 
TTGACAGTGA GCTACATTCC TGGTTCGATA TCCTTAGCTG CATATACTGT GTCAAAGGAA 
GGAATTGAGT GGGGATCAAA AAACATGGAT ATCAACAGTG ATGAAGCTAT CGGGTATGAA 
CCTTCTATGG CTGAAAAATG TCAGCTGCTA TTGTCCGATA GAATTCAAGG ATTTTTCTTG 
GTACCAGAAG AGGGCGTATG GAATTATAAT TTCAATGGTG CAAGCTTCAG TCCTAAAATG 
ACGTACAGTT TAAAACTTGA TGTCCCTCTG CCATTCTTTG CTTTAGAGCA TAGACCTACT 
CATGTAATAA GCTATACTGA ACTGGAGACG AATGACAGAT TAGAGGAAGA TATGCCAGAT 
GCATTTGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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spp42 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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