Prp8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prp8
Entrez Gene ID	36304
Full Name	pre-mRNA processing factor 8
Synonyms	A1Z8U0,CG8877,Dmel\CG8877,E-2e,prp8
General protein information	Preferred Names pre-mRNA processing factor 8 Names CG8877 gene product from transcript CG8877-RA CG8877-PA Prp8-PA U5 small nuclear ribonucleoprotein-specific factor prp8
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 48E3-48E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_136891.4	NP_610735.1	pre-mRNA processing factor 8

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prpf8	NP_001178519.1
Neurospora crassa	NCU07832	XP_962953.1
Arabidopsis thaliana (thale cress)	SUS2	NP_178124.2
	PRPF8	XP_001117328.1
Canis lupus familiaris (dog)	PRPF8	XP_854384.1
Drosophila melanogaster (fruit fly)	Prp8	NP_610735.1
Caenorhabditis elegans (roundworm)	prp-8	NP_498785.1
Schizosaccharomyces pombe (fission yeast)	spp42	NP_593861.1
Magnaporthe oryzae (rice blast fungus)	MGG_05244	XP_003712798.1
Oryza sativa (rice)	Os06g0167000	NP_001056917.1
Homo sapiens (human)	PRPF8	NP_006436.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006885	XP_308873.2
Kluyveromyces lactis	KLLA0A05280g	XP_451233.1
Eremothecium gossypii	AGOS_ABR207W	NP_983156.2
Bos taurus (cattle)	PRPF8	NP_001099108.1
Mus musculus (house mouse)	Prpf8	NP_619600.2
Arabidopsis thaliana (thale cress)	AT4G38780	NP_195589.2
Xenopus tropicalis (tropical clawed frog)	prpf8	XP_002936105.1
Gallus gallus (chicken)	PRPF8	XP_415805.2
Danio rerio (zebrafish)	prpf8	NP_957270.2
Saccharomyces cerevisiae (baker's yeast)	PRP8	NP_012035.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Prp8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prp8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa09689	NM_136891.4 Latest version!	Drosophila melanogaster pre-mRNA processing factor 8 (Prp8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prp8 ( NM_136891.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_610735.1 Drosophila melanogaster pre-mRNA processing factor 8 (Prp8), mRNA.

Price & Availability↑

CloneID	OFa09689
Clone ID Related Accession (Same CDS sequence)	NM_136891.4
Accession Version	NM_136891.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pre-mRNA processing factor 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_136891.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCATTC CGCCGTACAT GATACCACAA AATGCGTGGG CAGCCCAGCT GATGGCCCAG 
CAGGCCTACG CGGCTGCCCA TGCTCAGCAG GCTCAACTGC ACGCCCAGCA GCAGATGGCC 
AACCAAATTC AGCAGATTCC GCCACCGGGG GCTCCGCTGC CGCCGGCCGG TGGCCATACC 
AACGGTATAC CCATCCCGGT GGGAGGTCAG GGACCCGGAT TGGGCCAGAT TCCCACGCCC 
AAGCCGGACA TACTCACCGA GGAGAAGCTG CAGGAGAAGG CGCTTAAATG GCAGCATCTG 
CAGTCCAAGC GATTCGCTGA GAAGCGCAAG TTTGGCTTCG TGGATACCCA GAAGGAGGAT 
ATGCCGCCGG AGCACATCAG GAAGATCATT CGCGATCACG GAGATATGAC GTCGCGCAAG 
TATCGTCATG ACAAGCGAGT ATATCTCGGC GCCCTGAAGT ACATGCCACA TGCTGTGCTC 
AAGCTGCTAG AGAACATGCC AATGCCATGG GAGCAGATCC GGGATGTCCA GGTGCTGTAC 
CACATCACTG GAGCCATTAC CTTTGTCAAT GAGATTCCCT GGGTTATTGA GCCCGTCTAC 
ATAGCACAAT GGGGTACAAT GTGGATCATG ATGCGGCGTG AGAAACGCGA TCGTCGCCAC 
TTTAAGCGCA TGAGATTCCC GCCATTTGAC GATGAAGAGC CGCCTCTAGA CTATGCCGAC 
AACGTGCTGG ACGTGGAGCC CTTGGAGGCC ATTCAAATTG AGCTGGACAA TGACGAAGAC 
AATGCTGTAT ACAAATGGTT TTATGACCAT CGTCCTCTAG TGGATACACA GTTTGTGAAC 
GGCACTACCT ACCGAAAGTG GAATCTTTCC CTGCCTCAGC TAGCCACGCT GTATCGATTG 
GCCAATCAAT TGCTCACCGA TCTAGTCGAC AATAACTTCT TTTATTTGTT TGACCCAAAA 
AGTTTCTTCA CCGCCAAGGC TCTTAATATG GCCATTCCAG GAGGACCTAA GTTTGAACCC 
CTCATTAAGG ACCACAATGT TGGGGACGAG GATTGGAACG AGTTCAATGA CATTAACAAA 
GTCATTATCC GGCAGCCAAT TCGCACGGAA TACCGCATTG CCTTTCCTTA CTTGTACAAC 
AATATGCCAC ACTTTGTGCA TTTGAGCTGG TATCACACTC CCAACGTGGT CTACATCAAG 
ACCGAAGATC CCGATCTGCC TGCATTTTAC TTTGATCCGC TCATCAATCC CATATCCCAT 
CGCAATGCAA ACTCGAAGAT TCAGGAGCCC CTACCTGATG ACGACGAGGA CTTTACTCTT 
CCGGACGACG TGCAGCCGTT CCTGCAGGAC ACTCCCCTGT ACACTGACAA CACTGCAAAC 
GGAATCGCCT TGCTGTGGGC ACCCCGCCCC TTTAACATGC GCTCTGGTCG CTCCCGTCGT 
GCCATCGATG TGCCGCTGGT CAAGTGCTGG TACAAGGAGC ATTGTCCACC TGGCCATCCG 
GTTAAGGTGC GCGTTAGCTA CCAGAAACTG CTCAAATACT ATGTGCTCAA TGCCCTAAAA 
CACCGCAAAC CAAAGCCACA AAAGAAACGC TACCTCTTCC GTTCGTTTAA GGCCACAAAA 
TTCTTCCAGA CCACAACTCT GGATTGGGTT GAGGCTGGAC TGCAAGTTTG TCGTCAGGGA 
TATAACATGT TGAATCTATT GATTCACCGA AAAAATCTTA ACTACTTGCA TTTAGATTAC 
AATTTCAACT TGAAGCCCGT TAAAACTCTG ACCACCAAGG AGCGTAAGAA GTCGCGATTC 
GGAAATGCTT TCCATTTGTG CCGTGAGATC CTGCGTCTGA CCAAGCTAAT CATTGACTCT 
CATGTTCAGT ATCGGCTAAA CAATGTGGAC GCCTTTCAGT TGGCTGACGG ACTGCAGTAC 
ATATTCGCCC ATGTTGGCCA GCTGACTGGC ATGTATCGCT ATAAATACAA GCTGATGAGG 
CAGATTCGAA TGTGTAAGGA TCTGAAACAT TTGATATACT ATCGCTTCAA CACGGGGCCC 
GTGGGCAAGG GACCCGGATG TGGCTTTTGG GCACCTGGAT GGCGAGTCTG GCTGTTCTTT 
ATGCGCGGCA TCACTCCGCT ACTGGAACGC TGGCTGGGCA ATCTATTGTC GCGTCAGTTC 
GAGGGTCGTC ACTCCAAGGG TGTGGCCAAG ACGGTGACTA AGCAGCGTGT TGAGTCCCAC 
TTTGATCTGG AGTTGCGTGC CTCCGTGATG CACGATATTG TGGACATGAT GCCCGAGGGC 
ATCAAGCAGA ACAAAGCTCG TACCATTTTG CAGCATTTAT CGGAGGCATG GCGTTGCTGG 
AAAGCGAACA TTCCGTGGAA GGTGCCTGGT CTACCCATTC CCATTGAGAA TATGATCCTG 
CGTTACGTGA AAATGAAAGC CGACTGGTGG ACAAACACGG CTCACTATAA TCGGGAACGT 
ATACGACGTG GAGCAACCGT CGATAAGACG GTGTGCAAGA AGAATCTGGG GCGACTAACG 
CGACTCTATC TGAAGGCGGA GCAGGAGAGG CAGCACAACT ACTTAAAGGA CGGTCCGTAC 
ATATCACCGG AGGAGGCTGT GGCTATTTAC ACAACCACGG TCCATTGGCT GGAATCACGT 
CGCTTTGCGC CCATTCCCTT CCCACCACTT TCATACAAAC ACGATACCAA GCTGCTAATA 
TTGGCCCTTG AGAGGCTAAA AGAGGCGTAT AGCGTTAAGT CCAGGCTGAA TCAAAGTCAG 
CGTGAGGAGC TGGGCCTCAT CGAACAGGCC TACGATAATC CTCACGAGGC GTTGTCCCGC 
ATCAAGCGTC ATCTGCTCAC GCAGCGAGCC TTCAAGGAGG TGGGCATTGA GTTCATGGAC 
TTGTACAGCC ATCTGATTCC AGTCTACGAA GTGGAACCCC TGGAAAAGAT TACAGACGCA 
TATTTGGATC AGTATCTGTG GTATGAGGCT GACAAGCGTC GATTGTTCCC GCCATGGATC 
AAGCCCAGCG ACACGGAGCC GCCACCATTG TTGGCCTACA AGTGGTGTCA AGGCATTAAC 
AATCTGCAGG ATGTTTGGGA TGTGGGTGAG GGCGAGTGCA ATGTGCTGCT GGAGTCTAGA 
TTTGAAAAGC TGTACGAGAA AATTGACTTG ACGCTGCTTA ATCGTTTGCT CCGCCTGATT 
GTCGATCATA ATATCGCCGA TTACATGACG GCCAAGAACA ACGTTGTGAT CAACTACAAG 
GACATGAACC ACACGAACAG CTACGGCATC ATTCGCGGTC TGCAGTTCTC ATCGTTCATC 
ACACAGTACT ATGGACTGGT GCTAGATCTC TTGGTTTTGG GTCTTCATCG ATCCAGCGAG 
ATGGCTGGCC CACCCCAAAT GCCGAACGAT TTCCTCACGT TCCAGGACAC AGTCACAGAA 
ACGGCACATC CTATTCGATT GTACTGCCGC TATGTCGATC GTATTCACTT GTTCTTCAGA 
TTCTCAGCGG AGGAGGCTCG CGATCTGATC CAGCGCTATC TTACCGAGCA TCCGGATCCG 
AACAACGAAA ACATTGTGGG CTACAATAAC AAGAAGTGCT GGCCACGCGA CGCTCGTATG 
CGTTTAATGA AGCACGATGT TAACTTGGGT CGCGCAGTCT TCTGGGACAT TAAGAACCGA 
CTACCTCGCT CTGTGACCAC CATTGGTTGG GAGAGCACTT TCGTTTCGGT GTATTCGAAG 
GACAACCCCA ATCTGTTGTT CAACATGAGT GGATTTGAGT GTCGCATTTT GCCAAAGTGT 
CGCACTCAGA ATGAAGAGTT CACCCATCGT GATGGTGTTT GGAATTTACA GAACGAAATC 
ACCAAGGAAC GTACTGCCCA GTGCTTCCTG CGTGTGGACG ATGAGTCATT GGGTCGTTTC 
CATAATCGTG TTCGACAGAT CCTGATGGCC TCTGGGTCCA CTACGTTCAC AAAGATTGTG 
AACAAATGGA ACACAGCGCT CATAGGCTTG ATGACCTACT TCCGTGAGGC TGTGGTCAAT 
ACCCAGGAAT TGTTGGACCT GTTGGTCAAG TGCGAGAACA AAATTCAGAC TCGTATCAAG 
ATTGGCCTCA ACTCTAAGAT GCCTTCCCGT TTCCCGCCTG TTGTGTTCTA CACTCCCAAA 
GAACTGGGCG GTCTGGGAAT GTTGAGCATG GGTCACGTCT TGATTCCGCA ATCCGATCTG 
CGTTGGTCCA AGCAAACTGA TGTGGGCATA ACCCATTTCC GCTCCGGTAT GTCCCACGAT 
GAGGACCAGC TTATTCCCAA TCTGTATCGC TACATTCAGC CGTGGGAGAG TGAGTTTATA 
GACTCGCAGC GCGTGTGGGC GGAGTACGCA TTGAAGCGTC AGGAGGCGAA TGCCCAGAAC 
CGTAGGCTCA CACTGGAGGA TTTAGAGGAC AGCTGGGATC GCGGTATTCC ACGTATCAAC 
ACACTATTCC AAAAGGATCG TCACACACTG GCCTACGACA AGGGCTGGCG CATTCGTACC 
GAATTCAAAC AATACCAGGT GCTAAAGCAG AATCCCTTCT GGTGGACGCA TCAGCGTCAC 
GATGGCAAGC TGTGGAACCT GAACAATTAT CGCACGGATA TGATCCAGGC GTTGGGTGGA 
GTGGAGGGCA TACTGGAGCA CACGCTGTTC AAGGGCACCT ACTTCCCCAC GTGGGAGGGT 
CTTTTCTGGG AGAAGGCCTC CGGTTTCGAA GAGTCAATGA AGTACAAGAA GTTGACCAAT 
GCTCAGCGAT CGGGTCTCAA CCAGATTCCG AATCGTCGAT TCACACTTTG GTGGTCACCG 
ACTATCAATC GAGCGAATGT GTACGTCGGT TTCCAGGTGC AACTGGATCT GACTGGCATA 
TTCATGCACG GCAAGATTCC GACACTAAAG ATTTCGCTGA TTCAAATATT CCGAGCCCAT 
TTGTGGCAGA AGATCCACGA ATCGATTGTC ATGGATTTGT GTCAGGTATT CGATCAGGAA 
CTGGACGCCT TGGAAATCGA GACGGTGCAG AAGGAAACCA TCCATCCACG TAAGTCCTAC 
AAGATGAACT CATCGTGTGC CGACATCCTG CTCTTCCCCG CCTACAAATG GAATGTGTCG 
CGACCTTCAC TGCTGGCCGA CACCAAGGAC ACCATGGATA ACACCACCAC CCAAAAGTAC 
TGGCTGGATA TCCAGTTGCG TTGGGGCGAT TATGACTCGC ACGATGTGGA GCGCTATGCC 
AGGGCCAAGT TCCTCGATTA CACCACTGAC AACATGTCCA TCTATCCCTC ACCGACGGGC 
GTGTTGATTG CCATCGATTT GGCATACAAT CTGCATTCGG CCTACGGCAA TTGGTTCCCC 
GGCTGTAAGA CACTCATTCA GCAGGCCATG GCCAAAATCA TGAAGGCAAA CCCCGCCCTC 
TATGTGCTGC GTGAGCGCAT CCGCAAGGCG TTGCAGCTGT ACTCTTCGGA GCCCACAGAG 
CCATACCTAA GTTCGCAGAA TTACGGAGAA CTGTTCTCGA ATCAGATTAT CTGGTTTGTG 
GATGACACCA ACGTGTATCG CGTCACCATA CACAAGACAT TTGAGGGAAA TCTGACAACG 
AAGCCCATTA ATGGAGCAAT TTTCATCTTT AATCCGCGAA CGGGTCAGCT CTTCCTTAAG 
ATCATTCACA CCTCTGTGTG GGCTGGCCAG AAGCGTTTGG GCCAACTGGC CAAGTGGAAG 
ACTGCTGAGG AAGTGGCCGC TTTGATTCGA TCATTGCCGG TTGAGGAGCA GCCCAAACAA 
ATTATTGTGA CGCGCAAGGG CATGTTGGAC CCGCTGGAGG TGCACTTGCT CGACTTCCCT 
AACATTGTGA TCAAGGGCTC CGAGCTTCAG CTACCGTTCC AGGCTTGTCT CAAAGTCGAG 
AAGTTTGGTG ACCTCATTCT GAAGGCTACC GAGCCACAGA TGGTGCTCTT CAACTTGTAC 
GACGATTGGC TGAAGACCAT ATCTTCATAT ACGGCTTTCA GTCGACTTAT CCTCATTCTG 
CGCGCTCTTC ACGTCAATAC GGAGAGGACA AAGATCATCC TGAAGCCGGA CAAGACCACC 
ATAACGGAGG CGCATCACAT TTGGCCCACT CTGACCGACG AGGAATGGAT TAAGGTGGAA 
GTGCAGTTGA AGGACCTCAT TCTCGCCGAT TACGGCAAGA AGAACAACGT AAATGTGGCT 
TCGCTGACTC AGTCCGAGAT CCGTGACATC ATTCTGGGCA TGGAGATCAG TGCGCCCTCA 
GCGCAGCGCC AGCAGATTGC TGAGATCGAA AAGCAGACCA AGGAGCAAAA CCAACTGACG 
GCCACCACTA CGCGTACCAC AAACAAGCAT GGTGACGAAA TTATTACCTC CACTACATCC 
AATTACGAAA CGCAGACGTT CAGCTCGAAG ACAGAGTGGC GAGTAAGAGC TATCTCCGCC 
ACTAATCTGC ACCTGCGAAC GAACCACATC TACGTGAGTT CGGACGACAT AAAGGAGACC 
GGCTACACCT ACATCCTGCC CAAGAACATA CTCAAGAAGT TTGTCACGAT CTCGGATCTG 
CGGGCCCAGA TAGCTGGCTA CTTGTATGGT GTAAGTCCAC CGGACAATCC GCAGGTAAAG 
GAGATTCGCT GCATCGTAAT GCCGCCGCAA TGGGGCACTC ACCAGACGAT CAACTTGCCC 
AACACCCTGC CCACACATCA GTATCTCAAG GACATGGAGC CGCTAGGCTG GATCCACACG 
CAGCCCAACG AGCTGCCCCA ATTGTCACCG CAGGACATTA CGACGCACGC GAAGATCATG 
CAGGAGAACT CCAACTGGGA TGGCGAGAAG ACGATCGTGA TCACGTGCTC CTTTACGCCC 
GGCTCCTGCT CACTTACGGC CTACAAGCTG ACGCCTTCGG GCTTCGAGTG GGGCTCCAAG 
AACACGGACA AAGGTAACAA TCCCAAGGGA TACCTGCCTT CGCACTACGA ACGCGTTCAG 
ATGCTGTTGT CGAACAAGTT CCTTGGGTTC TTCATGGTGC CGGCGCAAAG CAGCTGGAAC 
TACAACTTCA TGGGCGTGCG CCACGATCCC AACATGAAGT ACGAACTGCA GCTGGCCAAC 
CCGAAGGAGT TCTATCACGA GTTGCATCGC ACCTCGCATT TCCTGCTCTT CTCGAATCTC 
GAGGACGGCG GCGATGGCGC CGGTGCGGAT CGGGAGGATG TGTACGCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_610735.1
CDS	133..7323
Translation	MSIPPYMIPQNAWAAQLMAQQAYAAAHAQQAQLHAQQQMANQIQQIPPPGAPLPPAGGHTNGIPIPVGGQGPGLGQIPTPKPDILTEEKLQEKALKWQHLQSKRFAEKRKFGFVDTQKEDMPPEHIRKIIRDHGDMTSRKYRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVQVLYHITGAITFVNEIPWVIEPVYIAQWGTMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNVLDVEPLEAIQIELDNDEDNAVYKWFYDHRPLVDTQFVNGTTYRKWNLSLPQLATLYRLANQLLTDLVDNNFFYLFDPKSFFTAKALNMAIPGGPKFEPLIKDHNVGDEDWNEFNDINKVIIRQPIRTEYRIAFPYLYNNMPHFVHLSWYHTPNVVYIKTEDPDLPAFYFDPLINPISHRNANSKIQEPLPDDDEDFTLPDDVQPFLQDTPLYTDNTANGIALLWAPRPFNMRSGRSRRAIDVPLVKCWYKEHCPPGHPVKVRVSYQKLLKYYVLNALKHRKPKPQKKRYLFRSFKATKFFQTTTLDWVEAGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREILRLTKLIIDSHVQYRLNNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAPGWRVWLFFMRGITPLLERWLGNLLSRQFEGRHSKGVAKTVTKQRVESHFDLELRASVMHDIVDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPIPIENMILRYVKMKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYISPEEAVAIYTTTVHWLESRRFAPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLIPVYEVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPSDTEPPPLLAYKWCQGINNLQDVWDVGEGECNVLLESRFEKLYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFSSFITQYYGLVLDLLVLGLHRSSEMAGPPQMPNDFLTFQDTVTETAHPIRLYCRYVDRIHLFFRFSAEEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTIGWESTFVSVYSKDNPNLLFNMSGFECRILPKCRTQNEEFTHRDGVWNLQNEITKERTAQCFLRVDDESLGRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHDEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEANAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRIRTEFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKYKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFPAYKWNVSRPSLLADTKDTMDNTTTQKYWLDIQLRWGDYDSHDVERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGCKTLIQQAMAKIMKANPALYVLRERIRKALQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNTERTKIILKPDKTTITEAHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSAQRQQIAEIEKQTKEQNQLTATTTRTTNKHGDEIITSTTSNYETQTFSSKTEWRVRAISATNLHLRTNHIYVSSDDIKETGYTYILPKNILKKFVTISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMPPQWGTHQTINLPNTLPTHQYLKDMEPLGWIHTQPNELPQLSPQDITTHAKIMQENSNWDGEKTIVITCSFTPGSCSLTAYKLTPSGFEWGSKNTDKGNNPKGYLPSHYERVQMLLSNKFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHELHRTSHFLLFSNLEDGGDGAGADREDVYA

Target ORF information:

RefSeq Version	NM_136891.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pre-mRNA processing factor 8 (Prp8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136891.4

ORF Insert Sequence:

ATGTCCATTC CGCCGTACAT GATACCACAA AATGCGTGGG CAGCCCAGCT GATGGCCCAG 
CAGGCCTACG CGGCTGCCCA TGCTCAGCAG GCTCAACTGC ACGCCCAGCA GCAGATGGCC 
AACCAAATTC AGCAGATTCC GCCACCGGGG GCTCCGCTGC CGCCGGCCGG TGGCCATACC 
AACGGTATAC CCATCCCGGT GGGAGGTCAG GGACCCGGAT TGGGCCAGAT TCCCACGCCC 
AAGCCGGACA TACTCACCGA GGAGAAGCTG CAGGAGAAGG CGCTTAAATG GCAGCATCTG 
CAGTCCAAGC GATTCGCTGA GAAGCGCAAG TTTGGCTTCG TGGATACCCA GAAGGAGGAT 
ATGCCGCCGG AGCACATCAG GAAGATCATT CGCGATCACG GAGATATGAC GTCGCGCAAG 
TATCGTCATG ACAAGCGAGT ATATCTCGGC GCCCTGAAGT ACATGCCACA TGCTGTGCTC 
AAGCTGCTAG AGAACATGCC AATGCCATGG GAGCAGATCC GGGATGTCCA GGTGCTGTAC 
CACATCACTG GAGCCATTAC CTTTGTCAAT GAGATTCCCT GGGTTATTGA GCCCGTCTAC 
ATAGCACAAT GGGGTACAAT GTGGATCATG ATGCGGCGTG AGAAACGCGA TCGTCGCCAC 
TTTAAGCGCA TGAGATTCCC GCCATTTGAC GATGAAGAGC CGCCTCTAGA CTATGCCGAC 
AACGTGCTGG ACGTGGAGCC CTTGGAGGCC ATTCAAATTG AGCTGGACAA TGACGAAGAC 
AATGCTGTAT ACAAATGGTT TTATGACCAT CGTCCTCTAG TGGATACACA GTTTGTGAAC 
GGCACTACCT ACCGAAAGTG GAATCTTTCC CTGCCTCAGC TAGCCACGCT GTATCGATTG 
GCCAATCAAT TGCTCACCGA TCTAGTCGAC AATAACTTCT TTTATTTGTT TGACCCAAAA 
AGTTTCTTCA CCGCCAAGGC TCTTAATATG GCCATTCCAG GAGGACCTAA GTTTGAACCC 
CTCATTAAGG ACCACAATGT TGGGGACGAG GATTGGAACG AGTTCAATGA CATTAACAAA 
GTCATTATCC GGCAGCCAAT TCGCACGGAA TACCGCATTG CCTTTCCTTA CTTGTACAAC 
AATATGCCAC ACTTTGTGCA TTTGAGCTGG TATCACACTC CCAACGTGGT CTACATCAAG 
ACCGAAGATC CCGATCTGCC TGCATTTTAC TTTGATCCGC TCATCAATCC CATATCCCAT 
CGCAATGCAA ACTCGAAGAT TCAGGAGCCC CTACCTGATG ACGACGAGGA CTTTACTCTT 
CCGGACGACG TGCAGCCGTT CCTGCAGGAC ACTCCCCTGT ACACTGACAA CACTGCAAAC 
GGAATCGCCT TGCTGTGGGC ACCCCGCCCC TTTAACATGC GCTCTGGTCG CTCCCGTCGT 
GCCATCGATG TGCCGCTGGT CAAGTGCTGG TACAAGGAGC ATTGTCCACC TGGCCATCCG 
GTTAAGGTGC GCGTTAGCTA CCAGAAACTG CTCAAATACT ATGTGCTCAA TGCCCTAAAA 
CACCGCAAAC CAAAGCCACA AAAGAAACGC TACCTCTTCC GTTCGTTTAA GGCCACAAAA 
TTCTTCCAGA CCACAACTCT GGATTGGGTT GAGGCTGGAC TGCAAGTTTG TCGTCAGGGA 
TATAACATGT TGAATCTATT GATTCACCGA AAAAATCTTA ACTACTTGCA TTTAGATTAC 
AATTTCAACT TGAAGCCCGT TAAAACTCTG ACCACCAAGG AGCGTAAGAA GTCGCGATTC 
GGAAATGCTT TCCATTTGTG CCGTGAGATC CTGCGTCTGA CCAAGCTAAT CATTGACTCT 
CATGTTCAGT ATCGGCTAAA CAATGTGGAC GCCTTTCAGT TGGCTGACGG ACTGCAGTAC 
ATATTCGCCC ATGTTGGCCA GCTGACTGGC ATGTATCGCT ATAAATACAA GCTGATGAGG 
CAGATTCGAA TGTGTAAGGA TCTGAAACAT TTGATATACT ATCGCTTCAA CACGGGGCCC 
GTGGGCAAGG GACCCGGATG TGGCTTTTGG GCACCTGGAT GGCGAGTCTG GCTGTTCTTT 
ATGCGCGGCA TCACTCCGCT ACTGGAACGC TGGCTGGGCA ATCTATTGTC GCGTCAGTTC 
GAGGGTCGTC ACTCCAAGGG TGTGGCCAAG ACGGTGACTA AGCAGCGTGT TGAGTCCCAC 
TTTGATCTGG AGTTGCGTGC CTCCGTGATG CACGATATTG TGGACATGAT GCCCGAGGGC 
ATCAAGCAGA ACAAAGCTCG TACCATTTTG CAGCATTTAT CGGAGGCATG GCGTTGCTGG 
AAAGCGAACA TTCCGTGGAA GGTGCCTGGT CTACCCATTC CCATTGAGAA TATGATCCTG 
CGTTACGTGA AAATGAAAGC CGACTGGTGG ACAAACACGG CTCACTATAA TCGGGAACGT 
ATACGACGTG GAGCAACCGT CGATAAGACG GTGTGCAAGA AGAATCTGGG GCGACTAACG 
CGACTCTATC TGAAGGCGGA GCAGGAGAGG CAGCACAACT ACTTAAAGGA CGGTCCGTAC 
ATATCACCGG AGGAGGCTGT GGCTATTTAC ACAACCACGG TCCATTGGCT GGAATCACGT 
CGCTTTGCGC CCATTCCCTT CCCACCACTT TCATACAAAC ACGATACCAA GCTGCTAATA 
TTGGCCCTTG AGAGGCTAAA AGAGGCGTAT AGCGTTAAGT CCAGGCTGAA TCAAAGTCAG 
CGTGAGGAGC TGGGCCTCAT CGAACAGGCC TACGATAATC CTCACGAGGC GTTGTCCCGC 
ATCAAGCGTC ATCTGCTCAC GCAGCGAGCC TTCAAGGAGG TGGGCATTGA GTTCATGGAC 
TTGTACAGCC ATCTGATTCC AGTCTACGAA GTGGAACCCC TGGAAAAGAT TACAGACGCA 
TATTTGGATC AGTATCTGTG GTATGAGGCT GACAAGCGTC GATTGTTCCC GCCATGGATC 
AAGCCCAGCG ACACGGAGCC GCCACCATTG TTGGCCTACA AGTGGTGTCA AGGCATTAAC 
AATCTGCAGG ATGTTTGGGA TGTGGGTGAG GGCGAGTGCA ATGTGCTGCT GGAGTCTAGA 
TTTGAAAAGC TGTACGAGAA AATTGACTTG ACGCTGCTTA ATCGTTTGCT CCGCCTGATT 
GTCGATCATA ATATCGCCGA TTACATGACG GCCAAGAACA ACGTTGTGAT CAACTACAAG 
GACATGAACC ACACGAACAG CTACGGCATC ATTCGCGGTC TGCAGTTCTC ATCGTTCATC 
ACACAGTACT ATGGACTGGT GCTAGATCTC TTGGTTTTGG GTCTTCATCG ATCCAGCGAG 
ATGGCTGGCC CACCCCAAAT GCCGAACGAT TTCCTCACGT TCCAGGACAC AGTCACAGAA 
ACGGCACATC CTATTCGATT GTACTGCCGC TATGTCGATC GTATTCACTT GTTCTTCAGA 
TTCTCAGCGG AGGAGGCTCG CGATCTGATC CAGCGCTATC TTACCGAGCA TCCGGATCCG 
AACAACGAAA ACATTGTGGG CTACAATAAC AAGAAGTGCT GGCCACGCGA CGCTCGTATG 
CGTTTAATGA AGCACGATGT TAACTTGGGT CGCGCAGTCT TCTGGGACAT TAAGAACCGA 
CTACCTCGCT CTGTGACCAC CATTGGTTGG GAGAGCACTT TCGTTTCGGT GTATTCGAAG 
GACAACCCCA ATCTGTTGTT CAACATGAGT GGATTTGAGT GTCGCATTTT GCCAAAGTGT 
CGCACTCAGA ATGAAGAGTT CACCCATCGT GATGGTGTTT GGAATTTACA GAACGAAATC 
ACCAAGGAAC GTACTGCCCA GTGCTTCCTG CGTGTGGACG ATGAGTCATT GGGTCGTTTC 
CATAATCGTG TTCGACAGAT CCTGATGGCC TCTGGGTCCA CTACGTTCAC AAAGATTGTG 
AACAAATGGA ACACAGCGCT CATAGGCTTG ATGACCTACT TCCGTGAGGC TGTGGTCAAT 
ACCCAGGAAT TGTTGGACCT GTTGGTCAAG TGCGAGAACA AAATTCAGAC TCGTATCAAG 
ATTGGCCTCA ACTCTAAGAT GCCTTCCCGT TTCCCGCCTG TTGTGTTCTA CACTCCCAAA 
GAACTGGGCG GTCTGGGAAT GTTGAGCATG GGTCACGTCT TGATTCCGCA ATCCGATCTG 
CGTTGGTCCA AGCAAACTGA TGTGGGCATA ACCCATTTCC GCTCCGGTAT GTCCCACGAT 
GAGGACCAGC TTATTCCCAA TCTGTATCGC TACATTCAGC CGTGGGAGAG TGAGTTTATA 
GACTCGCAGC GCGTGTGGGC GGAGTACGCA TTGAAGCGTC AGGAGGCGAA TGCCCAGAAC 
CGTAGGCTCA CACTGGAGGA TTTAGAGGAC AGCTGGGATC GCGGTATTCC ACGTATCAAC 
ACACTATTCC AAAAGGATCG TCACACACTG GCCTACGACA AGGGCTGGCG CATTCGTACC 
GAATTCAAAC AATACCAGGT GCTAAAGCAG AATCCCTTCT GGTGGACGCA TCAGCGTCAC 
GATGGCAAGC TGTGGAACCT GAACAATTAT CGCACGGATA TGATCCAGGC GTTGGGTGGA 
GTGGAGGGCA TACTGGAGCA CACGCTGTTC AAGGGCACCT ACTTCCCCAC GTGGGAGGGT 
CTTTTCTGGG AGAAGGCCTC CGGTTTCGAA GAGTCAATGA AGTACAAGAA GTTGACCAAT 
GCTCAGCGAT CGGGTCTCAA CCAGATTCCG AATCGTCGAT TCACACTTTG GTGGTCACCG 
ACTATCAATC GAGCGAATGT GTACGTCGGT TTCCAGGTGC AACTGGATCT GACTGGCATA 
TTCATGCACG GCAAGATTCC GACACTAAAG ATTTCGCTGA TTCAAATATT CCGAGCCCAT 
TTGTGGCAGA AGATCCACGA ATCGATTGTC ATGGATTTGT GTCAGGTATT CGATCAGGAA 
CTGGACGCCT TGGAAATCGA GACGGTGCAG AAGGAAACCA TCCATCCACG TAAGTCCTAC 
AAGATGAACT CATCGTGTGC CGACATCCTG CTCTTCCCCG CCTACAAATG GAATGTGTCG 
CGACCTTCAC TGCTGGCCGA CACCAAGGAC ACCATGGATA ACACCACCAC CCAAAAGTAC 
TGGCTGGATA TCCAGTTGCG TTGGGGCGAT TATGACTCGC ACGATGTGGA GCGCTATGCC 
AGGGCCAAGT TCCTCGATTA CACCACTGAC AACATGTCCA TCTATCCCTC ACCGACGGGC 
GTGTTGATTG CCATCGATTT GGCATACAAT CTGCATTCGG CCTACGGCAA TTGGTTCCCC 
GGCTGTAAGA CACTCATTCA GCAGGCCATG GCCAAAATCA TGAAGGCAAA CCCCGCCCTC 
TATGTGCTGC GTGAGCGCAT CCGCAAGGCG TTGCAGCTGT ACTCTTCGGA GCCCACAGAG 
CCATACCTAA GTTCGCAGAA TTACGGAGAA CTGTTCTCGA ATCAGATTAT CTGGTTTGTG 
GATGACACCA ACGTGTATCG CGTCACCATA CACAAGACAT TTGAGGGAAA TCTGACAACG 
AAGCCCATTA ATGGAGCAAT TTTCATCTTT AATCCGCGAA CGGGTCAGCT CTTCCTTAAG 
ATCATTCACA CCTCTGTGTG GGCTGGCCAG AAGCGTTTGG GCCAACTGGC CAAGTGGAAG 
ACTGCTGAGG AAGTGGCCGC TTTGATTCGA TCATTGCCGG TTGAGGAGCA GCCCAAACAA 
ATTATTGTGA CGCGCAAGGG CATGTTGGAC CCGCTGGAGG TGCACTTGCT CGACTTCCCT 
AACATTGTGA TCAAGGGCTC CGAGCTTCAG CTACCGTTCC AGGCTTGTCT CAAAGTCGAG 
AAGTTTGGTG ACCTCATTCT GAAGGCTACC GAGCCACAGA TGGTGCTCTT CAACTTGTAC 
GACGATTGGC TGAAGACCAT ATCTTCATAT ACGGCTTTCA GTCGACTTAT CCTCATTCTG 
CGCGCTCTTC ACGTCAATAC GGAGAGGACA AAGATCATCC TGAAGCCGGA CAAGACCACC 
ATAACGGAGG CGCATCACAT TTGGCCCACT CTGACCGACG AGGAATGGAT TAAGGTGGAA 
GTGCAGTTGA AGGACCTCAT TCTCGCCGAT TACGGCAAGA AGAACAACGT AAATGTGGCT 
TCGCTGACTC AGTCCGAGAT CCGTGACATC ATTCTGGGCA TGGAGATCAG TGCGCCCTCA 
GCGCAGCGCC AGCAGATTGC TGAGATCGAA AAGCAGACCA AGGAGCAAAA CCAACTGACG 
GCCACCACTA CGCGTACCAC AAACAAGCAT GGTGACGAAA TTATTACCTC CACTACATCC 
AATTACGAAA CGCAGACGTT CAGCTCGAAG ACAGAGTGGC GAGTAAGAGC TATCTCCGCC 
ACTAATCTGC ACCTGCGAAC GAACCACATC TACGTGAGTT CGGACGACAT AAAGGAGACC 
GGCTACACCT ACATCCTGCC CAAGAACATA CTCAAGAAGT TTGTCACGAT CTCGGATCTG 
CGGGCCCAGA TAGCTGGCTA CTTGTATGGT GTAAGTCCAC CGGACAATCC GCAGGTAAAG 
GAGATTCGCT GCATCGTAAT GCCGCCGCAA TGGGGCACTC ACCAGACGAT CAACTTGCCC 
AACACCCTGC CCACACATCA GTATCTCAAG GACATGGAGC CGCTAGGCTG GATCCACACG 
CAGCCCAACG AGCTGCCCCA ATTGTCACCG CAGGACATTA CGACGCACGC GAAGATCATG 
CAGGAGAACT CCAACTGGGA TGGCGAGAAG ACGATCGTGA TCACGTGCTC CTTTACGCCC 
GGCTCCTGCT CACTTACGGC CTACAAGCTG ACGCCTTCGG GCTTCGAGTG GGGCTCCAAG 
AACACGGACA AAGGTAACAA TCCCAAGGGA TACCTGCCTT CGCACTACGA ACGCGTTCAG 
ATGCTGTTGT CGAACAAGTT CCTTGGGTTC TTCATGGTGC CGGCGCAAAG CAGCTGGAAC 
TACAACTTCA TGGGCGTGCG CCACGATCCC AACATGAAGT ACGAACTGCA GCTGGCCAAC 
CCGAAGGAGT TCTATCACGA GTTGCATCGC ACCTCGCATT TCCTGCTCTT CTCGAATCTC 
GAGGACGGCG GCGATGGCGC CGGTGCGGAT CGGGAGGATG TGTACGCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Prp8 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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