PRPF8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRPF8
Entrez Gene ID	10594
Full Name	pre-mRNA processing factor 8
Synonyms	HPRP8,PRP8,PRPC8,RP13,SNRNP220
General protein information	Preferred Names pre-mRNA processing factor 8 Names pre-mRNA-processing-splicing factor 8 220 kDa U5 snRNP-specific protein PRP8 homolog PRP8 pre-mRNA processing factor 8 homolog U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p apoptosis-regulated protein 1 apoptosis-regulated protein 2 p220 precursor mRNA processing protein splicing factor Prp8
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.3
Summary	Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008].
Disorder MIM:	607300
Disorder Html:	Retinitis pigmentosa 13, 600059 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_006445.3	NP_006436.3	pre-mRNA-processing-splicing factor 8
XM_024450537.1	XP_024306305.1	pre-mRNA-processing-splicing factor 8 isoform X1
NM_006445.4	NP_006436.3	pre-mRNA-processing-splicing factor 8

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prpf8	NP_001178519.1
Neurospora crassa	NCU07832	XP_962953.1
Arabidopsis thaliana (thale cress)	SUS2	NP_178124.2
	PRPF8	XP_001117328.1
Canis lupus familiaris (dog)	PRPF8	XP_854384.1
Drosophila melanogaster (fruit fly)	Prp8	NP_610735.1
Caenorhabditis elegans (roundworm)	prp-8	NP_498785.1
Schizosaccharomyces pombe (fission yeast)	spp42	NP_593861.1
Magnaporthe oryzae (rice blast fungus)	MGG_05244	XP_003712798.1
Oryza sativa (rice)	Os06g0167000	NP_001056917.1
Homo sapiens (human)	PRPF8	NP_006436.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006885	XP_308873.2
Kluyveromyces lactis	KLLA0A05280g	XP_451233.1
Eremothecium gossypii	AGOS_ABR207W	NP_983156.2
Bos taurus (cattle)	PRPF8	NP_001099108.1
Mus musculus (house mouse)	Prpf8	NP_619600.2
Arabidopsis thaliana (thale cress)	AT4G38780	NP_195589.2
Xenopus tropicalis (tropical clawed frog)	prpf8	XP_002936105.1
Gallus gallus (chicken)	PRPF8	XP_415805.2
Danio rerio (zebrafish)	prpf8	NP_957270.2
Saccharomyces cerevisiae (baker's yeast)	PRP8	NP_012035.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure of a human catalytic step I spliceosome.
Zhan X, Yan C, Zhang X, Lei J, Shi Y
Science (New York, N.Y.)(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HSP90/R2TP chaperone system promotes the assembly of a key module of U5 snRNP while assuring the quality control of PRPF8. The proteomics data further reveal new interactions between R2TP and the tuberous sclerosis complex, pointing to a potential link between growth signals and the assembly of key cellular machines.
Title: Assembly of the U5 snRNP component PRPF8 is controlled by the HSP90/R2TP chaperones.

influenza A virus upregulates cellular PRPF8 gene expression through viral NS1 protein and influenza virus polymerase basic protein 1 to increase virus production.
Title: Influenza A virus upregulates PRPF8 gene expression to increase virus production.

We show that PRP31, a component of U4 snRNP, is modified with K63-linked ubiquitin chains by the PRP19 complex and deubiquitinated by USP15 and its substrate targeting factor SART3. USP15SART3 makes a complex with USP4 and this ternary complex serves as a platform to deubiquitinate PRP31 and PRP3
Title: USP15 regulates dynamic protein-protein interactions of the spliceosome through deubiquitination of PRP31.

Most importantly between Prp8 and nucleotides at the exon-intron junction.
Title: Stable tri-snRNP integration is accompanied by a major structural rearrangement of the spliceosome that is dependent on Prp8 interaction with the 5' splice site.

Our findings exemplify the regulatory potential of changes in the core spliceosome machinery, which may be relevant to slow-onset human genetic diseases linked to PRPF8 deficiency
Title: Regulation of constitutive and alternative mRNA splicing across the human transcriptome by PRPF8 is determined by 5' splice site strength.

The following PRPF8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRPF8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19527	NM_006445.4 Latest version!	Homo sapiens pre-mRNA processing factor 8 (PRPF8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu19527	XM_024450537.1 Latest version!	Homo sapiens pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
View Old Accession Versions >>
OHu19527	NM_006445.3	Homo sapiens pre-mRNA processing factor 8 (PRPF8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRPF8 ( NM_006445.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006436.3 Homo sapiens pre-mRNA processing factor 8 (PRPF8), mRNA.

Price & Availability↑

CloneID	OHu19527
Clone ID Related Accession (Same CDS sequence)	NM_006445.3 , XM_024450537.1 , NM_006445.4
Accession Version	NM_006445.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD698473.1, DB081408.1 and BC064370.1. This sequence is a reference standard in the RefSeqGene project. On or before Aug 31, 2013 this sequence version replaced XM_005256414.1, NM_006445.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064370.1, SRR1803615.266153.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGAG TGTTTCCTTA TCGAGGGCCG GGTAACCCGG TGCCTGGCCC TCTAGCCCCG 
CTACCGGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CTCGAAAATG GCAGCAATTG 
CAGGCCAAGC GCTATGCAGA AAAGCGGAAG TTTGGGTTTG TGGATGCCCA GAAGGAAGAC 
ATGCCCCCAG AACATGTCAG GAAGATCATT CGAGACCATG GAGACATGAC CAACAGGAAG 
TTCCGCCATG ACAAAAGGGT TTACTTGGGT GCCCTAAAGT ACATGCCCCA CGCAGTCCTC 
AAACTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC 
CACATCACTG GAGCCATTTC CTTCGTCAAT GAGATTCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGCCGAG AAAAAAGAGA TAGGAGGCAT 
TTCAAGAGGA TGCGTTTTCC CCCTTTTGAT GATGAGGAGC CGCCCTTGGA CTATGCTGAC 
AACATCCTAG ATGTTGAGCC ACTGGAGGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAC 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCGTTGA GGGACAGCAG GAAGTATGTA 
AATGGCTCCA CTTACCAGCG CTGGCAGTTC ACACTACCTA TGATGTCGAC TCTCTACCGC 
CTGGCTAATC AGCTCCTGAC AGACTTGGTG GATGACAACT ACTTCTACCT GTTTGATTTG 
AAGGCCTTCT TTACGTCCAA GGCACTCAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAA 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGATATTAAC 
AAGATTATCA TCCGGCAGCC TATCCGCACT GAGTACAAGA TTGCTTTTCC TTACTTGTAC 
AACAATCTTC CACACCATGT CCACCTCACC TGGTACCATA CTCCCAATGT TGTATTCATC 
AAAACTGAGG ATCCTGACTT GCCAGCTTTC TACTTTGACC CTTTGATCAA CCCAATCTCC 
CATAGGCACT CAGTCAAGAG CCAGGAACCA TTGCCGGATG ATGATGAGGA ATTTGAGCTC 
CCGGAGTTTG TGGAGCCCTT CCTGAAGGAC ACACCCCTCT ATACAGACAA TACAGCCAAT 
GGCATTGCCC TGCTCTGGGC CCCGCGGCCC TTCAACCTAC GCTCTGGTCG CACCCGTCGG 
GCCCTGGACA TACCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC CGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTGAAG 
CATCGGCCCC CTAAGGCTCA AAAGAAGAGG TATTTGTTCC GCTCCTTCAA AGCCACCAAA 
TTCTTTCAGT CCACAAAGCT GGACTGGGTG GAGGTTGGGC TCCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGC AAAAACCTCA ACTACCTGCA CCTGGACTAC 
AACTTCAACC TCAAGCCTGT GAAAACGCTC ACCACCAAGG AAAGAAAGAA ATCTCGTTTT 
GGGAATGCTT TCCACCTGTG TCGGGAAGTT CTGCGTTTGA CTAAGCTGGT GGTGGATAGT 
CACGTGCAGT ATCGGCTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG ATTGCAGTAT 
ATATTTGCCC ATGTTGGGCA GTTGACGGGC ATGTATCGAT ACAAATACAA GCTGATGCGA 
CAGATTCGCA TGTGCAAGGA CCTGAAGCAT CTCATCTATT ATCGTTTCAA CACGGGCCCT 
GTAGGGAAGG GTCCTGGCTG TGGCTTCTGG GCTGCCGGTT GGCGAGTCTG GCTCTTTTTC 
ATGCGTGGCA TTACCCCTTT ATTAGAGCGA TGGCTTGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGAC ACTCAAAGGG GGTGGCAAAG ACAGTAACAA AGCAGCGAGT GGAGTCACAT 
TTTGACCTTG AGCTGCGGGC AGCTGTGATG CATGATATTC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ACAAGGCCCG GACAATCCTG CAGCACCTCA GTGAAGCCTG GCGCTGCTGG 
AAAGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCGACGC CCATAGAGAA TATGATCCTT 
CGATACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACTG CCCACTACAA CCGAGAACGG 
ATCCGCCGAG GGGCCACTGT GGACAAGACT GTTTGTAAAA AGAATCTGGG CCGCCTCACC 
CGGCTCTATC TGAAGGCAGA ACAGGAGCGG CAGCACAACT ACCTGAAGGA CGGGCCTTAC 
ATCACAGCGG AGGAAGCAGT GGCAGTATAT ACCACCACAG TGCATTGGTT GGAAAGCCGC 
AGGTTTTCAC CCATCCCATT CCCCCCACTC TCCTATAAGC ATGACACCAA GTTGCTCATC 
TTGGCATTGG AGCGGCTCAA GGAAGCTTAT AGTGTGAAGT CTCGGTTGAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TACGATAACC CCCACGAGGC GCTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC ACAGAGAGCC TTCAAAGAGG TGGGCATTGA GTTCATGGAT 
CTGTATAGCC ACCTCGTTCC AGTATATGAT GTTGAGCCCC TGGAGAAGAT AACTGATGCT 
TACCTGGACC AGTACCTGTG GTATGAAGCC GACAAGCGCC GCCTGTTCCC ACCCTGGATT 
AAGCCTGCAG ACACAGAACC ACCTCCGCTG CTTGTTTACA AGTGGTGTCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAA GGCGAGTGCA ATGTCATGCT GGAATCCCGC 
TTTGAGAAGA TGTATGAGAA GATCGACTTG ACTCTGCTCA ACAGGCTGCT GCGCCTCATC 
GTGGACCACA ACATAGCCGA CTACATGACA GCCAAGAACA ACGTCGTCAT CAACTATAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATCAGAGGCC TGCAGTTTGC CTCATTCATC 
GTGCAGTATT ATGGCCTGGT GATGGATTTG CTTGTATTGG GATTGCACCG GGCCAGTGAG 
ATGGCTGGGC CCCCTCAGAT GCCAAATGAC TTTCTCAGTT TCCAGGACAT AGCCACTGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTTTTCAGG 
TTCACAGCAG ATGAGGCTCG GGACCTGATT CAACGTTACC TGACAGAGCA CCCTGACCCC 
AATAATGAAA ACATCGTTGG CTATAATAAC AAGAAGTGCT GGCCCCGAGA TGCCCGCATG 
CGCCTCATGA AACATGATGT TAACTTAGGC CGGGCGGTAT TCTGGGACAT CAAGAACCGC 
TTGCCACGGT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG 
GACAACCCCA ACCTGCTGTT CAACATGTGT GGCTTCGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTGCA GAATGAGGTT 
ACTAAGGAGC GCACAGCTCA GTGTTTCCTG CGTGTGGACG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGTCAGAT TCTCATGGCC TCTGGGTCCA CCACCTTCAC CAAGATTGTG 
AATAAGTGGA ATACAGCTCT CATTGGCCTT ATGACATACT TTCGGGAGGC TGTGGTGAAC 
ACCCAAGAGC TCTTGGACTT ACTGGTGAAG TGTGAGAACA AAATCCAGAC ACGTATCAAG 
ATTGGACTCA ACTCCAAGAT GCCAAGTCGG TTCCCCCCGG TTGTGTTCTA CACCCCTAAG 
GAGTTGGGTG GACTCGGCAT GCTCTCAATG GGCCATGTGC TCATCCCCCA ATCCGACCTC 
AGGTGGTCCA AACAGACAGA TGTAGGTATC ACACACTTTC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TCATTCCCAA CTTGTACCGC TACATACAGC CATGGGAGAG CGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTACGCA CTCAAGAGGC AAGAGGCCAT TGCTCAGAAC 
AGACGCCTGA CTTTAGAAGA CCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAT 
ACCCTCTTCC AGAAGGACCG GCACACACTG GCTTATGATA AGGGCTGGCG TGTCAGAACT 
GACTTTAAGC AGTATCAGGT TTTGAAGCAG AATCCGTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACAGACA TGATCCAGGC CCTGGGCGGT 
GTGGAAGGCA TTCTGGAACA CACACTCTTT AAGGGCACTT ACTTCCCTAC CTGGGAGGGG 
CTTTTCTGGG AGAAGGCCAG TGGCTTTGAG GAATCTATGA AGTGGAAGAA GCTAACTAAT 
GCTCAGCGAT CAGGACTGAA CCAGATTCCC AATCGTAGAT TCACCCTCTG GTGGTCCCCG 
ACCATTAATC GAGCCAATGT ATATGTAGGC TTTCAGGTGC AGCTAGACCT GACGGGTATC 
TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCATGA GAGCATTGTT ATGGACTTAT GTCAGGTGTT TGACCAGGAA 
CTTGATGCAC TGGAAATTGA GACAGTACAA AAGGAGACAA TCCATCCCCG AAAGTCATAT 
AAGATGAACT CTTCCTGTGC AGATATCCTG CTCTTTGCCT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCTGA CTCCAAGGAT GTGATGGACA GCACCACCAC CCAGAAATAC 
TGGATTGACA TCCAGTTGCG CTGGGGGGAC TATGATTCCC ACGACATTGA GCGCTACGCC 
CGGGCCAAGT TCCTGGACTA CACCACCGAC AACATGAGTA TCTACCCTTC GCCCACAGGT 
GTACTCATCG CCATTGACCT GGCCTATAAC TTGCACAGTG CCTATGGAAA CTGGTTCCCA 
GGCAGCAAGC CTCTCATACA ACAGGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCCTG 
TATGTGTTAC GTGAACGGAT CCGCAAGGGG CTACAGCTCT ATTCATCTGA ACCCACTGAG 
CCTTATTTGT CTTCTCAGAA CTATGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACCA ACGTCTACAG AGTGACTATT CACAAGACCT TTGAAGGGAA CTTGACAACC 
AAGCCCATCA ACGGAGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTCCTCAAG 
ATAATCCACA CGTCCGTGTG GGCGGGACAG AAGCGTTTGG GGCAGTTGGC TAAGTGGAAG 
ACAGCTGAGG AGGTGGCCGC CCTGATCCGA TCTCTGCCTG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CCAGGAAGGG CATGCTGGAC CCACTGGAGG TGCACTTACT GGACTTCCCC 
AATATTGTCA TCAAAGGATC GGAGCTCCAA CTCCCTTTCC AGGCGTGTCT CAAGGTGGAA 
AAATTCGGGG ATCTCATCCT TAAAGCCACT GAGCCCCAGA TGGTTCTCTT CAACCTCTAT 
GACGACTGGC TCAAGACTAT TTCATCTTAC ACGGCCTTCT CCCGTCTCAT CCTGATTCTG 
CGTGCCCTAC ATGTGAACAA CGATCGGGCA AAAGTGATCC TGAAGCCAGA CAAGACTACT 
ATTACAGAAC CACACCACAT CTGGCCCACT CTGACTGACG AAGAATGGAT CAAGGTCGAG 
GTGCAGCTCA AGGATCTGAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAACGTGGCA 
TCACTGACAC AATCAGAAAT TCGAGACATC ATCCTGGGTA TGGAGATCTC GGCACCGTCA 
CAGCAGCGGC AGCAGATCGC TGAGATCGAG AAGCAGACCA AGGAACAATC GCAGCTGACG 
GCAACACAGA CTCGCACTGT CAACAAGCAT GGCGATGAGA TCATCACCTC CACCACCAGC 
AACTATGAGA CCCAGACTTT CTCATCCAAG ACTGAGTGGA GGGTCAGGGC CATCTCTGCT 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGACGACAT CAAGGAGACT 
GGCTACACCT ACATCCTTCC CAAGAATGTG CTTAAGAAGT TCATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGATA CCTATATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATTGTGAT GGTGCCGCAG TGGGGCACTC ACCAGACCGT GCACCTGCCT 
GGCCAGCTGC CCCAGCATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAGCCCAATG AGTCCCCGCA GTTATCACCC CAGGATGTCA CCACCCATGC CAAGATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACATGCAG CTTCACGCCA 
GGCTCCTGTA CACTGACGGC CTACAAGCTG ACCCCCAGTG GCTACGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAGGGC TACCTGCCTT CACACTATGA GAGGGTGCAG 
ATGCTGCTGT CGGACCGTTT CCTTGGCTTC TTCATGGTCC CTGCCCAGTC CTCGTGGAAC 
TACAACTTCA TGGGTGTTCG GCATGACCCC AACATGAAAT ATGAGCTACA GCTGGCGAAC 
CCCAAAGAGT TCTACCACGA GGTGCACAGG CCCTCTCACT TCCTCAACTT TGCTCTCCTG 
CAGGAGGGGG AGGTTTACTC TGCGGATCGG GAGGACCTGT ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006436.3
CDS	115..7122
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	NM_006445.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pre-mRNA processing factor 8 (PRPF8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006445.3

ORF Insert Sequence:

ATGGCCGGAG TGTTTCCTTA TCGAGGGCCG GGTAACCCGG TGCCTGGCCC TCTAGCCCCG 
CTACCGGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CTCGAAAATG GCAGCAATTG 
CAGGCCAAGC GCTATGCAGA AAAGCGGAAG TTTGGGTTTG TGGATGCCCA GAAGGAAGAC 
ATGCCCCCAG AACATGTCAG GAAGATCATT CGAGACCATG GAGACATGAC CAACAGGAAG 
TTCCGCCATG ACAAAAGGGT TTACTTGGGT GCCCTAAAGT ACATGCCCCA CGCAGTCCTC 
AAACTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC 
CACATCACTG GAGCCATTTC CTTCGTCAAT GAGATTCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGCCGAG AAAAAAGAGA TAGGAGGCAT 
TTCAAGAGGA TGCGTTTTCC CCCTTTTGAT GATGAGGAGC CGCCCTTGGA CTATGCTGAC 
AACATCCTAG ATGTTGAGCC ACTGGAGGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAC 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCGTTGA GGGACAGCAG GAAGTATGTA 
AATGGCTCCA CTTACCAGCG CTGGCAGTTC ACACTACCTA TGATGTCGAC TCTCTACCGC 
CTGGCTAATC AGCTCCTGAC AGACTTGGTG GATGACAACT ACTTCTACCT GTTTGATTTG 
AAGGCCTTCT TTACGTCCAA GGCACTCAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAA 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGATATTAAC 
AAGATTATCA TCCGGCAGCC TATCCGCACT GAGTACAAGA TTGCTTTTCC TTACTTGTAC 
AACAATCTTC CACACCATGT CCACCTCACC TGGTACCATA CTCCCAATGT TGTATTCATC 
AAAACTGAGG ATCCTGACTT GCCAGCTTTC TACTTTGACC CTTTGATCAA CCCAATCTCC 
CATAGGCACT CAGTCAAGAG CCAGGAACCA TTGCCGGATG ATGATGAGGA ATTTGAGCTC 
CCGGAGTTTG TGGAGCCCTT CCTGAAGGAC ACACCCCTCT ATACAGACAA TACAGCCAAT 
GGCATTGCCC TGCTCTGGGC CCCGCGGCCC TTCAACCTAC GCTCTGGTCG CACCCGTCGG 
GCCCTGGACA TACCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC CGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTGAAG 
CATCGGCCCC CTAAGGCTCA AAAGAAGAGG TATTTGTTCC GCTCCTTCAA AGCCACCAAA 
TTCTTTCAGT CCACAAAGCT GGACTGGGTG GAGGTTGGGC TCCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGC AAAAACCTCA ACTACCTGCA CCTGGACTAC 
AACTTCAACC TCAAGCCTGT GAAAACGCTC ACCACCAAGG AAAGAAAGAA ATCTCGTTTT 
GGGAATGCTT TCCACCTGTG TCGGGAAGTT CTGCGTTTGA CTAAGCTGGT GGTGGATAGT 
CACGTGCAGT ATCGGCTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG ATTGCAGTAT 
ATATTTGCCC ATGTTGGGCA GTTGACGGGC ATGTATCGAT ACAAATACAA GCTGATGCGA 
CAGATTCGCA TGTGCAAGGA CCTGAAGCAT CTCATCTATT ATCGTTTCAA CACGGGCCCT 
GTAGGGAAGG GTCCTGGCTG TGGCTTCTGG GCTGCCGGTT GGCGAGTCTG GCTCTTTTTC 
ATGCGTGGCA TTACCCCTTT ATTAGAGCGA TGGCTTGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGAC ACTCAAAGGG GGTGGCAAAG ACAGTAACAA AGCAGCGAGT GGAGTCACAT 
TTTGACCTTG AGCTGCGGGC AGCTGTGATG CATGATATTC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ACAAGGCCCG GACAATCCTG CAGCACCTCA GTGAAGCCTG GCGCTGCTGG 
AAAGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCGACGC CCATAGAGAA TATGATCCTT 
CGATACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACTG CCCACTACAA CCGAGAACGG 
ATCCGCCGAG GGGCCACTGT GGACAAGACT GTTTGTAAAA AGAATCTGGG CCGCCTCACC 
CGGCTCTATC TGAAGGCAGA ACAGGAGCGG CAGCACAACT ACCTGAAGGA CGGGCCTTAC 
ATCACAGCGG AGGAAGCAGT GGCAGTATAT ACCACCACAG TGCATTGGTT GGAAAGCCGC 
AGGTTTTCAC CCATCCCATT CCCCCCACTC TCCTATAAGC ATGACACCAA GTTGCTCATC 
TTGGCATTGG AGCGGCTCAA GGAAGCTTAT AGTGTGAAGT CTCGGTTGAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TACGATAACC CCCACGAGGC GCTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC ACAGAGAGCC TTCAAAGAGG TGGGCATTGA GTTCATGGAT 
CTGTATAGCC ACCTCGTTCC AGTATATGAT GTTGAGCCCC TGGAGAAGAT AACTGATGCT 
TACCTGGACC AGTACCTGTG GTATGAAGCC GACAAGCGCC GCCTGTTCCC ACCCTGGATT 
AAGCCTGCAG ACACAGAACC ACCTCCGCTG CTTGTTTACA AGTGGTGTCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAA GGCGAGTGCA ATGTCATGCT GGAATCCCGC 
TTTGAGAAGA TGTATGAGAA GATCGACTTG ACTCTGCTCA ACAGGCTGCT GCGCCTCATC 
GTGGACCACA ACATAGCCGA CTACATGACA GCCAAGAACA ACGTCGTCAT CAACTATAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATCAGAGGCC TGCAGTTTGC CTCATTCATC 
GTGCAGTATT ATGGCCTGGT GATGGATTTG CTTGTATTGG GATTGCACCG GGCCAGTGAG 
ATGGCTGGGC CCCCTCAGAT GCCAAATGAC TTTCTCAGTT TCCAGGACAT AGCCACTGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTTTTCAGG 
TTCACAGCAG ATGAGGCTCG GGACCTGATT CAACGTTACC TGACAGAGCA CCCTGACCCC 
AATAATGAAA ACATCGTTGG CTATAATAAC AAGAAGTGCT GGCCCCGAGA TGCCCGCATG 
CGCCTCATGA AACATGATGT TAACTTAGGC CGGGCGGTAT TCTGGGACAT CAAGAACCGC 
TTGCCACGGT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG 
GACAACCCCA ACCTGCTGTT CAACATGTGT GGCTTCGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTGCA GAATGAGGTT 
ACTAAGGAGC GCACAGCTCA GTGTTTCCTG CGTGTGGACG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGTCAGAT TCTCATGGCC TCTGGGTCCA CCACCTTCAC CAAGATTGTG 
AATAAGTGGA ATACAGCTCT CATTGGCCTT ATGACATACT TTCGGGAGGC TGTGGTGAAC 
ACCCAAGAGC TCTTGGACTT ACTGGTGAAG TGTGAGAACA AAATCCAGAC ACGTATCAAG 
ATTGGACTCA ACTCCAAGAT GCCAAGTCGG TTCCCCCCGG TTGTGTTCTA CACCCCTAAG 
GAGTTGGGTG GACTCGGCAT GCTCTCAATG GGCCATGTGC TCATCCCCCA ATCCGACCTC 
AGGTGGTCCA AACAGACAGA TGTAGGTATC ACACACTTTC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TCATTCCCAA CTTGTACCGC TACATACAGC CATGGGAGAG CGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTACGCA CTCAAGAGGC AAGAGGCCAT TGCTCAGAAC 
AGACGCCTGA CTTTAGAAGA CCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAT 
ACCCTCTTCC AGAAGGACCG GCACACACTG GCTTATGATA AGGGCTGGCG TGTCAGAACT 
GACTTTAAGC AGTATCAGGT TTTGAAGCAG AATCCGTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACAGACA TGATCCAGGC CCTGGGCGGT 
GTGGAAGGCA TTCTGGAACA CACACTCTTT AAGGGCACTT ACTTCCCTAC CTGGGAGGGG 
CTTTTCTGGG AGAAGGCCAG TGGCTTTGAG GAATCTATGA AGTGGAAGAA GCTAACTAAT 
GCTCAGCGAT CAGGACTGAA CCAGATTCCC AATCGTAGAT TCACCCTCTG GTGGTCCCCG 
ACCATTAATC GAGCCAATGT ATATGTAGGC TTTCAGGTGC AGCTAGACCT GACGGGTATC 
TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCATGA GAGCATTGTT ATGGACTTAT GTCAGGTGTT TGACCAGGAA 
CTTGATGCAC TGGAAATTGA GACAGTACAA AAGGAGACAA TCCATCCCCG AAAGTCATAT 
AAGATGAACT CTTCCTGTGC AGATATCCTG CTCTTTGCCT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCTGA CTCCAAGGAT GTGATGGACA GCACCACCAC CCAGAAATAC 
TGGATTGACA TCCAGTTGCG CTGGGGGGAC TATGATTCCC ACGACATTGA GCGCTACGCC 
CGGGCCAAGT TCCTGGACTA CACCACCGAC AACATGAGTA TCTACCCTTC GCCCACAGGT 
GTACTCATCG CCATTGACCT GGCCTATAAC TTGCACAGTG CCTATGGAAA CTGGTTCCCA 
GGCAGCAAGC CTCTCATACA ACAGGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCCTG 
TATGTGTTAC GTGAACGGAT CCGCAAGGGG CTACAGCTCT ATTCATCTGA ACCCACTGAG 
CCTTATTTGT CTTCTCAGAA CTATGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACCA ACGTCTACAG AGTGACTATT CACAAGACCT TTGAAGGGAA CTTGACAACC 
AAGCCCATCA ACGGAGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTCCTCAAG 
ATAATCCACA CGTCCGTGTG GGCGGGACAG AAGCGTTTGG GGCAGTTGGC TAAGTGGAAG 
ACAGCTGAGG AGGTGGCCGC CCTGATCCGA TCTCTGCCTG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CCAGGAAGGG CATGCTGGAC CCACTGGAGG TGCACTTACT GGACTTCCCC 
AATATTGTCA TCAAAGGATC GGAGCTCCAA CTCCCTTTCC AGGCGTGTCT CAAGGTGGAA 
AAATTCGGGG ATCTCATCCT TAAAGCCACT GAGCCCCAGA TGGTTCTCTT CAACCTCTAT 
GACGACTGGC TCAAGACTAT TTCATCTTAC ACGGCCTTCT CCCGTCTCAT CCTGATTCTG 
CGTGCCCTAC ATGTGAACAA CGATCGGGCA AAAGTGATCC TGAAGCCAGA CAAGACTACT 
ATTACAGAAC CACACCACAT CTGGCCCACT CTGACTGACG AAGAATGGAT CAAGGTCGAG 
GTGCAGCTCA AGGATCTGAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAACGTGGCA 
TCACTGACAC AATCAGAAAT TCGAGACATC ATCCTGGGTA TGGAGATCTC GGCACCGTCA 
CAGCAGCGGC AGCAGATCGC TGAGATCGAG AAGCAGACCA AGGAACAATC GCAGCTGACG 
GCAACACAGA CTCGCACTGT CAACAAGCAT GGCGATGAGA TCATCACCTC CACCACCAGC 
AACTATGAGA CCCAGACTTT CTCATCCAAG ACTGAGTGGA GGGTCAGGGC CATCTCTGCT 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGACGACAT CAAGGAGACT 
GGCTACACCT ACATCCTTCC CAAGAATGTG CTTAAGAAGT TCATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGATA CCTATATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATTGTGAT GGTGCCGCAG TGGGGCACTC ACCAGACCGT GCACCTGCCT 
GGCCAGCTGC CCCAGCATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAGCCCAATG AGTCCCCGCA GTTATCACCC CAGGATGTCA CCACCCATGC CAAGATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACATGCAG CTTCACGCCA 
GGCTCCTGTA CACTGACGGC CTACAAGCTG ACCCCCAGTG GCTACGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAGGGC TACCTGCCTT CACACTATGA GAGGGTGCAG 
ATGCTGCTGT CGGACCGTTT CCTTGGCTTC TTCATGGTCC CTGCCCAGTC CTCGTGGAAC 
TACAACTTCA TGGGTGTTCG GCATGACCCC AACATGAAAT ATGAGCTACA GCTGGCGAAC 
CCCAAAGAGT TCTACCACGA GGTGCACAGG CCCTCTCACT TCCTCAACTT TGCTCTCCTG 
CAGGAGGGGG AGGTTTACTC TGCGGATCGG GAGGACCTGT ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF8 ( NM_006445.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006436.3 Homo sapiens pre-mRNA processing factor 8 (PRPF8), mRNA.

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CloneID	OHu19527
Clone ID Related Accession (Same CDS sequence)	NM_006445.3 , XM_024450537.1 , NM_006445.4
Accession Version	NM_006445.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	pre-mRNA-processing-splicing factor 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD698473.1, DB081408.1 and BC064370.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_006445.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064370.1, SRR1803615.266153.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000304992.11/ ENSP00000304350.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCGGAG TGTTTCCTTA TCGAGGGCCG GGTAACCCGG TGCCTGGCCC TCTAGCCCCG 
CTACCGGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CTCGAAAATG GCAGCAATTG 
CAGGCCAAGC GCTATGCAGA AAAGCGGAAG TTTGGGTTTG TGGATGCCCA GAAGGAAGAC 
ATGCCCCCAG AACATGTCAG GAAGATCATT CGAGACCATG GAGACATGAC CAACAGGAAG 
TTCCGCCATG ACAAAAGGGT TTACTTGGGT GCCCTAAAGT ACATGCCCCA CGCAGTCCTC 
AAACTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC 
CACATCACTG GAGCCATTTC CTTCGTCAAT GAGATTCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGCCGAG AAAAAAGAGA TAGGAGGCAT 
TTCAAGAGGA TGCGTTTTCC CCCTTTTGAT GATGAGGAGC CGCCCTTGGA CTATGCTGAC 
AACATCCTAG ATGTTGAGCC ACTGGAGGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAC 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCGTTGA GGGACAGCAG GAAGTATGTA 
AATGGCTCCA CTTACCAGCG CTGGCAGTTC ACACTACCTA TGATGTCGAC TCTCTACCGC 
CTGGCTAATC AGCTCCTGAC AGACTTGGTG GATGACAACT ACTTCTACCT GTTTGATTTG 
AAGGCCTTCT TTACGTCCAA GGCACTCAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAA 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGATATTAAC 
AAGATTATCA TCCGGCAGCC TATCCGCACT GAGTACAAGA TTGCTTTTCC TTACTTGTAC 
AACAATCTTC CACACCATGT CCACCTCACC TGGTACCATA CTCCCAATGT TGTATTCATC 
AAAACTGAGG ATCCTGACTT GCCAGCTTTC TACTTTGACC CTTTGATCAA CCCAATCTCC 
CATAGGCACT CAGTCAAGAG CCAGGAACCA TTGCCGGATG ATGATGAGGA ATTTGAGCTC 
CCGGAGTTTG TGGAGCCCTT CCTGAAGGAC ACACCCCTCT ATACAGACAA TACAGCCAAT 
GGCATTGCCC TGCTCTGGGC CCCGCGGCCC TTCAACCTAC GCTCTGGTCG CACCCGTCGG 
GCCCTGGACA TACCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC CGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTGAAG 
CATCGGCCCC CTAAGGCTCA AAAGAAGAGG TATTTGTTCC GCTCCTTCAA AGCCACCAAA 
TTCTTTCAGT CCACAAAGCT GGACTGGGTG GAGGTTGGGC TCCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGC AAAAACCTCA ACTACCTGCA CCTGGACTAC 
AACTTCAACC TCAAGCCTGT GAAAACGCTC ACCACCAAGG AAAGAAAGAA ATCTCGTTTT 
GGGAATGCTT TCCACCTGTG TCGGGAAGTT CTGCGTTTGA CTAAGCTGGT GGTGGATAGT 
CACGTGCAGT ATCGGCTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG ATTGCAGTAT 
ATATTTGCCC ATGTTGGGCA GTTGACGGGC ATGTATCGAT ACAAATACAA GCTGATGCGA 
CAGATTCGCA TGTGCAAGGA CCTGAAGCAT CTCATCTATT ATCGTTTCAA CACGGGCCCT 
GTAGGGAAGG GTCCTGGCTG TGGCTTCTGG GCTGCCGGTT GGCGAGTCTG GCTCTTTTTC 
ATGCGTGGCA TTACCCCTTT ATTAGAGCGA TGGCTTGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGAC ACTCAAAGGG GGTGGCAAAG ACAGTAACAA AGCAGCGAGT GGAGTCACAT 
TTTGACCTTG AGCTGCGGGC AGCTGTGATG CATGATATTC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ACAAGGCCCG GACAATCCTG CAGCACCTCA GTGAAGCCTG GCGCTGCTGG 
AAAGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCGACGC CCATAGAGAA TATGATCCTT 
CGATACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACTG CCCACTACAA CCGAGAACGG 
ATCCGCCGAG GGGCCACTGT GGACAAGACT GTTTGTAAAA AGAATCTGGG CCGCCTCACC 
CGGCTCTATC TGAAGGCAGA ACAGGAGCGG CAGCACAACT ACCTGAAGGA CGGGCCTTAC 
ATCACAGCGG AGGAAGCAGT GGCAGTATAT ACCACCACAG TGCATTGGTT GGAAAGCCGC 
AGGTTTTCAC CCATCCCATT CCCCCCACTC TCCTATAAGC ATGACACCAA GTTGCTCATC 
TTGGCATTGG AGCGGCTCAA GGAAGCTTAT AGTGTGAAGT CTCGGTTGAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TACGATAACC CCCACGAGGC GCTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC ACAGAGAGCC TTCAAAGAGG TGGGCATTGA GTTCATGGAT 
CTGTATAGCC ACCTCGTTCC AGTATATGAT GTTGAGCCCC TGGAGAAGAT AACTGATGCT 
TACCTGGACC AGTACCTGTG GTATGAAGCC GACAAGCGCC GCCTGTTCCC ACCCTGGATT 
AAGCCTGCAG ACACAGAACC ACCTCCGCTG CTTGTTTACA AGTGGTGTCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAA GGCGAGTGCA ATGTCATGCT GGAATCCCGC 
TTTGAGAAGA TGTATGAGAA GATCGACTTG ACTCTGCTCA ACAGGCTGCT GCGCCTCATC 
GTGGACCACA ACATAGCCGA CTACATGACA GCCAAGAACA ACGTCGTCAT CAACTATAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATCAGAGGCC TGCAGTTTGC CTCATTCATC 
GTGCAGTATT ATGGCCTGGT GATGGATTTG CTTGTATTGG GATTGCACCG GGCCAGTGAG 
ATGGCTGGGC CCCCTCAGAT GCCAAATGAC TTTCTCAGTT TCCAGGACAT AGCCACTGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTTTTCAGG 
TTCACAGCAG ATGAGGCTCG GGACCTGATT CAACGTTACC TGACAGAGCA CCCTGACCCC 
AATAATGAAA ACATCGTTGG CTATAATAAC AAGAAGTGCT GGCCCCGAGA TGCCCGCATG 
CGCCTCATGA AACATGATGT TAACTTAGGC CGGGCGGTAT TCTGGGACAT CAAGAACCGC 
TTGCCACGGT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG 
GACAACCCCA ACCTGCTGTT CAACATGTGT GGCTTCGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTGCA GAATGAGGTT 
ACTAAGGAGC GCACAGCTCA GTGTTTCCTG CGTGTGGACG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGTCAGAT TCTCATGGCC TCTGGGTCCA CCACCTTCAC CAAGATTGTG 
AATAAGTGGA ATACAGCTCT CATTGGCCTT ATGACATACT TTCGGGAGGC TGTGGTGAAC 
ACCCAAGAGC TCTTGGACTT ACTGGTGAAG TGTGAGAACA AAATCCAGAC ACGTATCAAG 
ATTGGACTCA ACTCCAAGAT GCCAAGTCGG TTCCCCCCGG TTGTGTTCTA CACCCCTAAG 
GAGTTGGGTG GACTCGGCAT GCTCTCAATG GGCCATGTGC TCATCCCCCA ATCCGACCTC 
AGGTGGTCCA AACAGACAGA TGTAGGTATC ACACACTTTC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TCATTCCCAA CTTGTACCGC TACATACAGC CATGGGAGAG CGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTACGCA CTCAAGAGGC AAGAGGCCAT TGCTCAGAAC 
AGACGCCTGA CTTTAGAAGA CCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAT 
ACCCTCTTCC AGAAGGACCG GCACACACTG GCTTATGATA AGGGCTGGCG TGTCAGAACT 
GACTTTAAGC AGTATCAGGT TTTGAAGCAG AATCCGTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACAGACA TGATCCAGGC CCTGGGCGGT 
GTGGAAGGCA TTCTGGAACA CACACTCTTT AAGGGCACTT ACTTCCCTAC CTGGGAGGGG 
CTTTTCTGGG AGAAGGCCAG TGGCTTTGAG GAATCTATGA AGTGGAAGAA GCTAACTAAT 
GCTCAGCGAT CAGGACTGAA CCAGATTCCC AATCGTAGAT TCACCCTCTG GTGGTCCCCG 
ACCATTAATC GAGCCAATGT ATATGTAGGC TTTCAGGTGC AGCTAGACCT GACGGGTATC 
TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCATGA GAGCATTGTT ATGGACTTAT GTCAGGTGTT TGACCAGGAA 
CTTGATGCAC TGGAAATTGA GACAGTACAA AAGGAGACAA TCCATCCCCG AAAGTCATAT 
AAGATGAACT CTTCCTGTGC AGATATCCTG CTCTTTGCCT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCTGA CTCCAAGGAT GTGATGGACA GCACCACCAC CCAGAAATAC 
TGGATTGACA TCCAGTTGCG CTGGGGGGAC TATGATTCCC ACGACATTGA GCGCTACGCC 
CGGGCCAAGT TCCTGGACTA CACCACCGAC AACATGAGTA TCTACCCTTC GCCCACAGGT 
GTACTCATCG CCATTGACCT GGCCTATAAC TTGCACAGTG CCTATGGAAA CTGGTTCCCA 
GGCAGCAAGC CTCTCATACA ACAGGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCCTG 
TATGTGTTAC GTGAACGGAT CCGCAAGGGG CTACAGCTCT ATTCATCTGA ACCCACTGAG 
CCTTATTTGT CTTCTCAGAA CTATGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACCA ACGTCTACAG AGTGACTATT CACAAGACCT TTGAAGGGAA CTTGACAACC 
AAGCCCATCA ACGGAGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTCCTCAAG 
ATAATCCACA CGTCCGTGTG GGCGGGACAG AAGCGTTTGG GGCAGTTGGC TAAGTGGAAG 
ACAGCTGAGG AGGTGGCCGC CCTGATCCGA TCTCTGCCTG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CCAGGAAGGG CATGCTGGAC CCACTGGAGG TGCACTTACT GGACTTCCCC 
AATATTGTCA TCAAAGGATC GGAGCTCCAA CTCCCTTTCC AGGCGTGTCT CAAGGTGGAA 
AAATTCGGGG ATCTCATCCT TAAAGCCACT GAGCCCCAGA TGGTTCTCTT CAACCTCTAT 
GACGACTGGC TCAAGACTAT TTCATCTTAC ACGGCCTTCT CCCGTCTCAT CCTGATTCTG 
CGTGCCCTAC ATGTGAACAA CGATCGGGCA AAAGTGATCC TGAAGCCAGA CAAGACTACT 
ATTACAGAAC CACACCACAT CTGGCCCACT CTGACTGACG AAGAATGGAT CAAGGTCGAG 
GTGCAGCTCA AGGATCTGAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAACGTGGCA 
TCACTGACAC AATCAGAAAT TCGAGACATC ATCCTGGGTA TGGAGATCTC GGCACCGTCA 
CAGCAGCGGC AGCAGATCGC TGAGATCGAG AAGCAGACCA AGGAACAATC GCAGCTGACG 
GCAACACAGA CTCGCACTGT CAACAAGCAT GGCGATGAGA TCATCACCTC CACCACCAGC 
AACTATGAGA CCCAGACTTT CTCATCCAAG ACTGAGTGGA GGGTCAGGGC CATCTCTGCT 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGACGACAT CAAGGAGACT 
GGCTACACCT ACATCCTTCC CAAGAATGTG CTTAAGAAGT TCATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGATA CCTATATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATTGTGAT GGTGCCGCAG TGGGGCACTC ACCAGACCGT GCACCTGCCT 
GGCCAGCTGC CCCAGCATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAGCCCAATG AGTCCCCGCA GTTATCACCC CAGGATGTCA CCACCCATGC CAAGATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACATGCAG CTTCACGCCA 
GGCTCCTGTA CACTGACGGC CTACAAGCTG ACCCCCAGTG GCTACGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAGGGC TACCTGCCTT CACACTATGA GAGGGTGCAG 
ATGCTGCTGT CGGACCGTTT CCTTGGCTTC TTCATGGTCC CTGCCCAGTC CTCGTGGAAC 
TACAACTTCA TGGGTGTTCG GCATGACCCC AACATGAAAT ATGAGCTACA GCTGGCGAAC 
CCCAAAGAGT TCTACCACGA GGTGCACAGG CCCTCTCACT TCCTCAACTT TGCTCTCCTG 
CAGGAGGGGG AGGTTTACTC TGCGGATCGG GAGGACCTGT ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006436.3
CDS	100..7107
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	NM_006445.4
Organism	Homo sapiens(human)
Definition	Homo sapiens pre-mRNA processing factor 8 (PRPF8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006445.4

ORF Insert Sequence:

ATGGCCGGAG TGTTTCCTTA TCGAGGGCCG GGTAACCCGG TGCCTGGCCC TCTAGCCCCG 
CTACCGGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CTCGAAAATG GCAGCAATTG 
CAGGCCAAGC GCTATGCAGA AAAGCGGAAG TTTGGGTTTG TGGATGCCCA GAAGGAAGAC 
ATGCCCCCAG AACATGTCAG GAAGATCATT CGAGACCATG GAGACATGAC CAACAGGAAG 
TTCCGCCATG ACAAAAGGGT TTACTTGGGT GCCCTAAAGT ACATGCCCCA CGCAGTCCTC 
AAACTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC 
CACATCACTG GAGCCATTTC CTTCGTCAAT GAGATTCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGCCGAG AAAAAAGAGA TAGGAGGCAT 
TTCAAGAGGA TGCGTTTTCC CCCTTTTGAT GATGAGGAGC CGCCCTTGGA CTATGCTGAC 
AACATCCTAG ATGTTGAGCC ACTGGAGGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAC 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCGTTGA GGGACAGCAG GAAGTATGTA 
AATGGCTCCA CTTACCAGCG CTGGCAGTTC ACACTACCTA TGATGTCGAC TCTCTACCGC 
CTGGCTAATC AGCTCCTGAC AGACTTGGTG GATGACAACT ACTTCTACCT GTTTGATTTG 
AAGGCCTTCT TTACGTCCAA GGCACTCAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAA 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGATATTAAC 
AAGATTATCA TCCGGCAGCC TATCCGCACT GAGTACAAGA TTGCTTTTCC TTACTTGTAC 
AACAATCTTC CACACCATGT CCACCTCACC TGGTACCATA CTCCCAATGT TGTATTCATC 
AAAACTGAGG ATCCTGACTT GCCAGCTTTC TACTTTGACC CTTTGATCAA CCCAATCTCC 
CATAGGCACT CAGTCAAGAG CCAGGAACCA TTGCCGGATG ATGATGAGGA ATTTGAGCTC 
CCGGAGTTTG TGGAGCCCTT CCTGAAGGAC ACACCCCTCT ATACAGACAA TACAGCCAAT 
GGCATTGCCC TGCTCTGGGC CCCGCGGCCC TTCAACCTAC GCTCTGGTCG CACCCGTCGG 
GCCCTGGACA TACCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC CGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTGAAG 
CATCGGCCCC CTAAGGCTCA AAAGAAGAGG TATTTGTTCC GCTCCTTCAA AGCCACCAAA 
TTCTTTCAGT CCACAAAGCT GGACTGGGTG GAGGTTGGGC TCCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGC AAAAACCTCA ACTACCTGCA CCTGGACTAC 
AACTTCAACC TCAAGCCTGT GAAAACGCTC ACCACCAAGG AAAGAAAGAA ATCTCGTTTT 
GGGAATGCTT TCCACCTGTG TCGGGAAGTT CTGCGTTTGA CTAAGCTGGT GGTGGATAGT 
CACGTGCAGT ATCGGCTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG ATTGCAGTAT 
ATATTTGCCC ATGTTGGGCA GTTGACGGGC ATGTATCGAT ACAAATACAA GCTGATGCGA 
CAGATTCGCA TGTGCAAGGA CCTGAAGCAT CTCATCTATT ATCGTTTCAA CACGGGCCCT 
GTAGGGAAGG GTCCTGGCTG TGGCTTCTGG GCTGCCGGTT GGCGAGTCTG GCTCTTTTTC 
ATGCGTGGCA TTACCCCTTT ATTAGAGCGA TGGCTTGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGAC ACTCAAAGGG GGTGGCAAAG ACAGTAACAA AGCAGCGAGT GGAGTCACAT 
TTTGACCTTG AGCTGCGGGC AGCTGTGATG CATGATATTC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ACAAGGCCCG GACAATCCTG CAGCACCTCA GTGAAGCCTG GCGCTGCTGG 
AAAGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCGACGC CCATAGAGAA TATGATCCTT 
CGATACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACTG CCCACTACAA CCGAGAACGG 
ATCCGCCGAG GGGCCACTGT GGACAAGACT GTTTGTAAAA AGAATCTGGG CCGCCTCACC 
CGGCTCTATC TGAAGGCAGA ACAGGAGCGG CAGCACAACT ACCTGAAGGA CGGGCCTTAC 
ATCACAGCGG AGGAAGCAGT GGCAGTATAT ACCACCACAG TGCATTGGTT GGAAAGCCGC 
AGGTTTTCAC CCATCCCATT CCCCCCACTC TCCTATAAGC ATGACACCAA GTTGCTCATC 
TTGGCATTGG AGCGGCTCAA GGAAGCTTAT AGTGTGAAGT CTCGGTTGAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TACGATAACC CCCACGAGGC GCTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC ACAGAGAGCC TTCAAAGAGG TGGGCATTGA GTTCATGGAT 
CTGTATAGCC ACCTCGTTCC AGTATATGAT GTTGAGCCCC TGGAGAAGAT AACTGATGCT 
TACCTGGACC AGTACCTGTG GTATGAAGCC GACAAGCGCC GCCTGTTCCC ACCCTGGATT 
AAGCCTGCAG ACACAGAACC ACCTCCGCTG CTTGTTTACA AGTGGTGTCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAA GGCGAGTGCA ATGTCATGCT GGAATCCCGC 
TTTGAGAAGA TGTATGAGAA GATCGACTTG ACTCTGCTCA ACAGGCTGCT GCGCCTCATC 
GTGGACCACA ACATAGCCGA CTACATGACA GCCAAGAACA ACGTCGTCAT CAACTATAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATCAGAGGCC TGCAGTTTGC CTCATTCATC 
GTGCAGTATT ATGGCCTGGT GATGGATTTG CTTGTATTGG GATTGCACCG GGCCAGTGAG 
ATGGCTGGGC CCCCTCAGAT GCCAAATGAC TTTCTCAGTT TCCAGGACAT AGCCACTGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTTTTCAGG 
TTCACAGCAG ATGAGGCTCG GGACCTGATT CAACGTTACC TGACAGAGCA CCCTGACCCC 
AATAATGAAA ACATCGTTGG CTATAATAAC AAGAAGTGCT GGCCCCGAGA TGCCCGCATG 
CGCCTCATGA AACATGATGT TAACTTAGGC CGGGCGGTAT TCTGGGACAT CAAGAACCGC 
TTGCCACGGT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG 
GACAACCCCA ACCTGCTGTT CAACATGTGT GGCTTCGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTGCA GAATGAGGTT 
ACTAAGGAGC GCACAGCTCA GTGTTTCCTG CGTGTGGACG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGTCAGAT TCTCATGGCC TCTGGGTCCA CCACCTTCAC CAAGATTGTG 
AATAAGTGGA ATACAGCTCT CATTGGCCTT ATGACATACT TTCGGGAGGC TGTGGTGAAC 
ACCCAAGAGC TCTTGGACTT ACTGGTGAAG TGTGAGAACA AAATCCAGAC ACGTATCAAG 
ATTGGACTCA ACTCCAAGAT GCCAAGTCGG TTCCCCCCGG TTGTGTTCTA CACCCCTAAG 
GAGTTGGGTG GACTCGGCAT GCTCTCAATG GGCCATGTGC TCATCCCCCA ATCCGACCTC 
AGGTGGTCCA AACAGACAGA TGTAGGTATC ACACACTTTC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TCATTCCCAA CTTGTACCGC TACATACAGC CATGGGAGAG CGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTACGCA CTCAAGAGGC AAGAGGCCAT TGCTCAGAAC 
AGACGCCTGA CTTTAGAAGA CCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAT 
ACCCTCTTCC AGAAGGACCG GCACACACTG GCTTATGATA AGGGCTGGCG TGTCAGAACT 
GACTTTAAGC AGTATCAGGT TTTGAAGCAG AATCCGTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACAGACA TGATCCAGGC CCTGGGCGGT 
GTGGAAGGCA TTCTGGAACA CACACTCTTT AAGGGCACTT ACTTCCCTAC CTGGGAGGGG 
CTTTTCTGGG AGAAGGCCAG TGGCTTTGAG GAATCTATGA AGTGGAAGAA GCTAACTAAT 
GCTCAGCGAT CAGGACTGAA CCAGATTCCC AATCGTAGAT TCACCCTCTG GTGGTCCCCG 
ACCATTAATC GAGCCAATGT ATATGTAGGC TTTCAGGTGC AGCTAGACCT GACGGGTATC 
TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCATGA GAGCATTGTT ATGGACTTAT GTCAGGTGTT TGACCAGGAA 
CTTGATGCAC TGGAAATTGA GACAGTACAA AAGGAGACAA TCCATCCCCG AAAGTCATAT 
AAGATGAACT CTTCCTGTGC AGATATCCTG CTCTTTGCCT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCTGA CTCCAAGGAT GTGATGGACA GCACCACCAC CCAGAAATAC 
TGGATTGACA TCCAGTTGCG CTGGGGGGAC TATGATTCCC ACGACATTGA GCGCTACGCC 
CGGGCCAAGT TCCTGGACTA CACCACCGAC AACATGAGTA TCTACCCTTC GCCCACAGGT 
GTACTCATCG CCATTGACCT GGCCTATAAC TTGCACAGTG CCTATGGAAA CTGGTTCCCA 
GGCAGCAAGC CTCTCATACA ACAGGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCCTG 
TATGTGTTAC GTGAACGGAT CCGCAAGGGG CTACAGCTCT ATTCATCTGA ACCCACTGAG 
CCTTATTTGT CTTCTCAGAA CTATGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACCA ACGTCTACAG AGTGACTATT CACAAGACCT TTGAAGGGAA CTTGACAACC 
AAGCCCATCA ACGGAGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTCCTCAAG 
ATAATCCACA CGTCCGTGTG GGCGGGACAG AAGCGTTTGG GGCAGTTGGC TAAGTGGAAG 
ACAGCTGAGG AGGTGGCCGC CCTGATCCGA TCTCTGCCTG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CCAGGAAGGG CATGCTGGAC CCACTGGAGG TGCACTTACT GGACTTCCCC 
AATATTGTCA TCAAAGGATC GGAGCTCCAA CTCCCTTTCC AGGCGTGTCT CAAGGTGGAA 
AAATTCGGGG ATCTCATCCT TAAAGCCACT GAGCCCCAGA TGGTTCTCTT CAACCTCTAT 
GACGACTGGC TCAAGACTAT TTCATCTTAC ACGGCCTTCT CCCGTCTCAT CCTGATTCTG 
CGTGCCCTAC ATGTGAACAA CGATCGGGCA AAAGTGATCC TGAAGCCAGA CAAGACTACT 
ATTACAGAAC CACACCACAT CTGGCCCACT CTGACTGACG AAGAATGGAT CAAGGTCGAG 
GTGCAGCTCA AGGATCTGAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAACGTGGCA 
TCACTGACAC AATCAGAAAT TCGAGACATC ATCCTGGGTA TGGAGATCTC GGCACCGTCA 
CAGCAGCGGC AGCAGATCGC TGAGATCGAG AAGCAGACCA AGGAACAATC GCAGCTGACG 
GCAACACAGA CTCGCACTGT CAACAAGCAT GGCGATGAGA TCATCACCTC CACCACCAGC 
AACTATGAGA CCCAGACTTT CTCATCCAAG ACTGAGTGGA GGGTCAGGGC CATCTCTGCT 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGACGACAT CAAGGAGACT 
GGCTACACCT ACATCCTTCC CAAGAATGTG CTTAAGAAGT TCATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGATA CCTATATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATTGTGAT GGTGCCGCAG TGGGGCACTC ACCAGACCGT GCACCTGCCT 
GGCCAGCTGC CCCAGCATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAGCCCAATG AGTCCCCGCA GTTATCACCC CAGGATGTCA CCACCCATGC CAAGATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACATGCAG CTTCACGCCA 
GGCTCCTGTA CACTGACGGC CTACAAGCTG ACCCCCAGTG GCTACGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAGGGC TACCTGCCTT CACACTATGA GAGGGTGCAG 
ATGCTGCTGT CGGACCGTTT CCTTGGCTTC TTCATGGTCC CTGCCCAGTC CTCGTGGAAC 
TACAACTTCA TGGGTGTTCG GCATGACCCC AACATGAAAT ATGAGCTACA GCTGGCGAAC 
CCCAAAGAGT TCTACCACGA GGTGCACAGG CCCTCTCACT TCCTCAACTT TGCTCTCCTG 
CAGGAGGGGG AGGTTTACTC TGCGGATCGG GAGGACCTGT ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRPF8 ( XM_024450537.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306305.1 Homo sapiens pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.

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CloneID	OHu19527
Clone ID Related Accession (Same CDS sequence)	NM_006445.3 , XM_024450537.1 , NM_006445.4
Accession Version	XM_024450537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pre-mRNA-processing-splicing factor 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG TGTTTCCTTA TCGAGGGCCG GGTAACCCGG TGCCTGGCCC TCTAGCCCCG 
CTACCGGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CTCGAAAATG GCAGCAATTG 
CAGGCCAAGC GCTATGCAGA AAAGCGGAAG TTTGGGTTTG TGGATGCCCA GAAGGAAGAC 
ATGCCCCCAG AACATGTCAG GAAGATCATT CGAGACCATG GAGACATGAC CAACAGGAAG 
TTCCGCCATG ACAAAAGGGT TTACTTGGGT GCCCTAAAGT ACATGCCCCA CGCAGTCCTC 
AAACTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC 
CACATCACTG GAGCCATTTC CTTCGTCAAT GAGATTCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGCCGAG AAAAAAGAGA TAGGAGGCAT 
TTCAAGAGGA TGCGTTTTCC CCCTTTTGAT GATGAGGAGC CGCCCTTGGA CTATGCTGAC 
AACATCCTAG ATGTTGAGCC ACTGGAGGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAC 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCGTTGA GGGACAGCAG GAAGTATGTA 
AATGGCTCCA CTTACCAGCG CTGGCAGTTC ACACTACCTA TGATGTCGAC TCTCTACCGC 
CTGGCTAATC AGCTCCTGAC AGACTTGGTG GATGACAACT ACTTCTACCT GTTTGATTTG 
AAGGCCTTCT TTACGTCCAA GGCACTCAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAA 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGATATTAAC 
AAGATTATCA TCCGGCAGCC TATCCGCACT GAGTACAAGA TTGCTTTTCC TTACTTGTAC 
AACAATCTTC CACACCATGT CCACCTCACC TGGTACCATA CTCCCAATGT TGTATTCATC 
AAAACTGAGG ATCCTGACTT GCCAGCTTTC TACTTTGACC CTTTGATCAA CCCAATCTCC 
CATAGGCACT CAGTCAAGAG CCAGGAACCA TTGCCGGATG ATGATGAGGA ATTTGAGCTC 
CCGGAGTTTG TGGAGCCCTT CCTGAAGGAC ACACCCCTCT ATACAGACAA TACAGCCAAT 
GGCATTGCCC TGCTCTGGGC CCCGCGGCCC TTCAACCTAC GCTCTGGTCG CACCCGTCGG 
GCCCTGGACA TACCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC CGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTGAAG 
CATCGGCCCC CTAAGGCTCA AAAGAAGAGG TATTTGTTCC GCTCCTTCAA AGCCACCAAA 
TTCTTTCAGT CCACAAAGCT GGACTGGGTG GAGGTTGGGC TCCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGC AAAAACCTCA ACTACCTGCA CCTGGACTAC 
AACTTCAACC TCAAGCCTGT GAAAACGCTC ACCACCAAGG AAAGAAAGAA ATCTCGTTTT 
GGGAATGCTT TCCACCTGTG TCGGGAAGTT CTGCGTTTGA CTAAGCTGGT GGTGGATAGT 
CACGTGCAGT ATCGGCTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG ATTGCAGTAT 
ATATTTGCCC ATGTTGGGCA GTTGACGGGC ATGTATCGAT ACAAATACAA GCTGATGCGA 
CAGATTCGCA TGTGCAAGGA CCTGAAGCAT CTCATCTATT ATCGTTTCAA CACGGGCCCT 
GTAGGGAAGG GTCCTGGCTG TGGCTTCTGG GCTGCCGGTT GGCGAGTCTG GCTCTTTTTC 
ATGCGTGGCA TTACCCCTTT ATTAGAGCGA TGGCTTGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGAC ACTCAAAGGG GGTGGCAAAG ACAGTAACAA AGCAGCGAGT GGAGTCACAT 
TTTGACCTTG AGCTGCGGGC AGCTGTGATG CATGATATTC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ACAAGGCCCG GACAATCCTG CAGCACCTCA GTGAAGCCTG GCGCTGCTGG 
AAAGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCGACGC CCATAGAGAA TATGATCCTT 
CGATACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACTG CCCACTACAA CCGAGAACGG 
ATCCGCCGAG GGGCCACTGT GGACAAGACT GTTTGTAAAA AGAATCTGGG CCGCCTCACC 
CGGCTCTATC TGAAGGCAGA ACAGGAGCGG CAGCACAACT ACCTGAAGGA CGGGCCTTAC 
ATCACAGCGG AGGAAGCAGT GGCAGTATAT ACCACCACAG TGCATTGGTT GGAAAGCCGC 
AGGTTTTCAC CCATCCCATT CCCCCCACTC TCCTATAAGC ATGACACCAA GTTGCTCATC 
TTGGCATTGG AGCGGCTCAA GGAAGCTTAT AGTGTGAAGT CTCGGTTGAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TACGATAACC CCCACGAGGC GCTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC ACAGAGAGCC TTCAAAGAGG TGGGCATTGA GTTCATGGAT 
CTGTATAGCC ACCTCGTTCC AGTATATGAT GTTGAGCCCC TGGAGAAGAT AACTGATGCT 
TACCTGGACC AGTACCTGTG GTATGAAGCC GACAAGCGCC GCCTGTTCCC ACCCTGGATT 
AAGCCTGCAG ACACAGAACC ACCTCCGCTG CTTGTTTACA AGTGGTGTCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAA GGCGAGTGCA ATGTCATGCT GGAATCCCGC 
TTTGAGAAGA TGTATGAGAA GATCGACTTG ACTCTGCTCA ACAGGCTGCT GCGCCTCATC 
GTGGACCACA ACATAGCCGA CTACATGACA GCCAAGAACA ACGTCGTCAT CAACTATAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATCAGAGGCC TGCAGTTTGC CTCATTCATC 
GTGCAGTATT ATGGCCTGGT GATGGATTTG CTTGTATTGG GATTGCACCG GGCCAGTGAG 
ATGGCTGGGC CCCCTCAGAT GCCAAATGAC TTTCTCAGTT TCCAGGACAT AGCCACTGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTTTTCAGG 
TTCACAGCAG ATGAGGCTCG GGACCTGATT CAACGTTACC TGACAGAGCA CCCTGACCCC 
AATAATGAAA ACATCGTTGG CTATAATAAC AAGAAGTGCT GGCCCCGAGA TGCCCGCATG 
CGCCTCATGA AACATGATGT TAACTTAGGC CGGGCGGTAT TCTGGGACAT CAAGAACCGC 
TTGCCACGGT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG 
GACAACCCCA ACCTGCTGTT CAACATGTGT GGCTTCGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTGCA GAATGAGGTT 
ACTAAGGAGC GCACAGCTCA GTGTTTCCTG CGTGTGGACG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGTCAGAT TCTCATGGCC TCTGGGTCCA CCACCTTCAC CAAGATTGTG 
AATAAGTGGA ATACAGCTCT CATTGGCCTT ATGACATACT TTCGGGAGGC TGTGGTGAAC 
ACCCAAGAGC TCTTGGACTT ACTGGTGAAG TGTGAGAACA AAATCCAGAC ACGTATCAAG 
ATTGGACTCA ACTCCAAGAT GCCAAGTCGG TTCCCCCCGG TTGTGTTCTA CACCCCTAAG 
GAGTTGGGTG GACTCGGCAT GCTCTCAATG GGCCATGTGC TCATCCCCCA ATCCGACCTC 
AGGTGGTCCA AACAGACAGA TGTAGGTATC ACACACTTTC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TCATTCCCAA CTTGTACCGC TACATACAGC CATGGGAGAG CGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTACGCA CTCAAGAGGC AAGAGGCCAT TGCTCAGAAC 
AGACGCCTGA CTTTAGAAGA CCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAT 
ACCCTCTTCC AGAAGGACCG GCACACACTG GCTTATGATA AGGGCTGGCG TGTCAGAACT 
GACTTTAAGC AGTATCAGGT TTTGAAGCAG AATCCGTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACAGACA TGATCCAGGC CCTGGGCGGT 
GTGGAAGGCA TTCTGGAACA CACACTCTTT AAGGGCACTT ACTTCCCTAC CTGGGAGGGG 
CTTTTCTGGG AGAAGGCCAG TGGCTTTGAG GAATCTATGA AGTGGAAGAA GCTAACTAAT 
GCTCAGCGAT CAGGACTGAA CCAGATTCCC AATCGTAGAT TCACCCTCTG GTGGTCCCCG 
ACCATTAATC GAGCCAATGT ATATGTAGGC TTTCAGGTGC AGCTAGACCT GACGGGTATC 
TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCATGA GAGCATTGTT ATGGACTTAT GTCAGGTGTT TGACCAGGAA 
CTTGATGCAC TGGAAATTGA GACAGTACAA AAGGAGACAA TCCATCCCCG AAAGTCATAT 
AAGATGAACT CTTCCTGTGC AGATATCCTG CTCTTTGCCT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCTGA CTCCAAGGAT GTGATGGACA GCACCACCAC CCAGAAATAC 
TGGATTGACA TCCAGTTGCG CTGGGGGGAC TATGATTCCC ACGACATTGA GCGCTACGCC 
CGGGCCAAGT TCCTGGACTA CACCACCGAC AACATGAGTA TCTACCCTTC GCCCACAGGT 
GTACTCATCG CCATTGACCT GGCCTATAAC TTGCACAGTG CCTATGGAAA CTGGTTCCCA 
GGCAGCAAGC CTCTCATACA ACAGGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCCTG 
TATGTGTTAC GTGAACGGAT CCGCAAGGGG CTACAGCTCT ATTCATCTGA ACCCACTGAG 
CCTTATTTGT CTTCTCAGAA CTATGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACCA ACGTCTACAG AGTGACTATT CACAAGACCT TTGAAGGGAA CTTGACAACC 
AAGCCCATCA ACGGAGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTCCTCAAG 
ATAATCCACA CGTCCGTGTG GGCGGGACAG AAGCGTTTGG GGCAGTTGGC TAAGTGGAAG 
ACAGCTGAGG AGGTGGCCGC CCTGATCCGA TCTCTGCCTG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CCAGGAAGGG CATGCTGGAC CCACTGGAGG TGCACTTACT GGACTTCCCC 
AATATTGTCA TCAAAGGATC GGAGCTCCAA CTCCCTTTCC AGGCGTGTCT CAAGGTGGAA 
AAATTCGGGG ATCTCATCCT TAAAGCCACT GAGCCCCAGA TGGTTCTCTT CAACCTCTAT 
GACGACTGGC TCAAGACTAT TTCATCTTAC ACGGCCTTCT CCCGTCTCAT CCTGATTCTG 
CGTGCCCTAC ATGTGAACAA CGATCGGGCA AAAGTGATCC TGAAGCCAGA CAAGACTACT 
ATTACAGAAC CACACCACAT CTGGCCCACT CTGACTGACG AAGAATGGAT CAAGGTCGAG 
GTGCAGCTCA AGGATCTGAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAACGTGGCA 
TCACTGACAC AATCAGAAAT TCGAGACATC ATCCTGGGTA TGGAGATCTC GGCACCGTCA 
CAGCAGCGGC AGCAGATCGC TGAGATCGAG AAGCAGACCA AGGAACAATC GCAGCTGACG 
GCAACACAGA CTCGCACTGT CAACAAGCAT GGCGATGAGA TCATCACCTC CACCACCAGC 
AACTATGAGA CCCAGACTTT CTCATCCAAG ACTGAGTGGA GGGTCAGGGC CATCTCTGCT 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGACGACAT CAAGGAGACT 
GGCTACACCT ACATCCTTCC CAAGAATGTG CTTAAGAAGT TCATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGATA CCTATATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATTGTGAT GGTGCCGCAG TGGGGCACTC ACCAGACCGT GCACCTGCCT 
GGCCAGCTGC CCCAGCATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAGCCCAATG AGTCCCCGCA GTTATCACCC CAGGATGTCA CCACCCATGC CAAGATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACATGCAG CTTCACGCCA 
GGCTCCTGTA CACTGACGGC CTACAAGCTG ACCCCCAGTG GCTACGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAGGGC TACCTGCCTT CACACTATGA GAGGGTGCAG 
ATGCTGCTGT CGGACCGTTT CCTTGGCTTC TTCATGGTCC CTGCCCAGTC CTCGTGGAAC 
TACAACTTCA TGGGTGTTCG GCATGACCCC AACATGAAAT ATGAGCTACA GCTGGCGAAC 
CCCAAAGAGT TCTACCACGA GGTGCACAGG CCCTCTCACT TCCTCAACTT TGCTCTCCTG 
CAGGAGGGGG AGGTTTACTC TGCGGATCGG GAGGACCTGT ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306305.1
CDS	98..7105
Translation	MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Target ORF information:

RefSeq Version	XM_024450537.1
Organism	Homo sapiens(human)
Definition	Homo sapiens pre-mRNA processing factor 8 (PRPF8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450537.1

ORF Insert Sequence:

ATGGCCGGAG TGTTTCCTTA TCGAGGGCCG GGTAACCCGG TGCCTGGCCC TCTAGCCCCG 
CTACCGGACT ACATGTCGGA GGAGAAGCTG CAGGAGAAAG CTCGAAAATG GCAGCAATTG 
CAGGCCAAGC GCTATGCAGA AAAGCGGAAG TTTGGGTTTG TGGATGCCCA GAAGGAAGAC 
ATGCCCCCAG AACATGTCAG GAAGATCATT CGAGACCATG GAGACATGAC CAACAGGAAG 
TTCCGCCATG ACAAAAGGGT TTACTTGGGT GCCCTAAAGT ACATGCCCCA CGCAGTCCTC 
AAACTCCTGG AGAACATGCC TATGCCTTGG GAGCAGATTC GGGATGTGCC TGTGCTGTAC 
CACATCACTG GAGCCATTTC CTTCGTCAAT GAGATTCCCT GGGTCATTGA ACCTGTCTAC 
ATCTCCCAGT GGGGGTCAAT GTGGATTATG ATGCGCCGAG AAAAAAGAGA TAGGAGGCAT 
TTCAAGAGGA TGCGTTTTCC CCCTTTTGAT GATGAGGAGC CGCCCTTGGA CTATGCTGAC 
AACATCCTAG ATGTTGAGCC ACTGGAGGCC ATTCAGCTAG AGCTGGACCC TGAGGAGGAC 
GCCCCTGTGT TGGACTGGTT CTATGACCAC CAGCCGTTGA GGGACAGCAG GAAGTATGTA 
AATGGCTCCA CTTACCAGCG CTGGCAGTTC ACACTACCTA TGATGTCGAC TCTCTACCGC 
CTGGCTAATC AGCTCCTGAC AGACTTGGTG GATGACAACT ACTTCTACCT GTTTGATTTG 
AAGGCCTTCT TTACGTCCAA GGCACTCAAT ATGGCCATTC CTGGAGGCCC CAAATTTGAA 
CCTCTTGTTC GAGACATCAA CCTACAGGAT GAAGACTGGA ATGAATTCAA TGATATTAAC 
AAGATTATCA TCCGGCAGCC TATCCGCACT GAGTACAAGA TTGCTTTTCC TTACTTGTAC 
AACAATCTTC CACACCATGT CCACCTCACC TGGTACCATA CTCCCAATGT TGTATTCATC 
AAAACTGAGG ATCCTGACTT GCCAGCTTTC TACTTTGACC CTTTGATCAA CCCAATCTCC 
CATAGGCACT CAGTCAAGAG CCAGGAACCA TTGCCGGATG ATGATGAGGA ATTTGAGCTC 
CCGGAGTTTG TGGAGCCCTT CCTGAAGGAC ACACCCCTCT ATACAGACAA TACAGCCAAT 
GGCATTGCCC TGCTCTGGGC CCCGCGGCCC TTCAACCTAC GCTCTGGTCG CACCCGTCGG 
GCCCTGGACA TACCCCTTGT CAAGAACTGG TATCGGGAGC ATTGTCCTGC CGGGCAGCCT 
GTGAAAGTGA GGGTCTCCTA CCAGAAGCTG CTTAAGTACT ATGTGCTGAA TGCCCTGAAG 
CATCGGCCCC CTAAGGCTCA AAAGAAGAGG TATTTGTTCC GCTCCTTCAA AGCCACCAAA 
TTCTTTCAGT CCACAAAGCT GGACTGGGTG GAGGTTGGGC TCCAGGTTTG CCGCCAGGGC 
TACAACATGC TCAACCTTCT CATTCACCGC AAAAACCTCA ACTACCTGCA CCTGGACTAC 
AACTTCAACC TCAAGCCTGT GAAAACGCTC ACCACCAAGG AAAGAAAGAA ATCTCGTTTT 
GGGAATGCTT TCCACCTGTG TCGGGAAGTT CTGCGTTTGA CTAAGCTGGT GGTGGATAGT 
CACGTGCAGT ATCGGCTGGG CAATGTGGAT GCCTTCCAGC TGGCAGATGG ATTGCAGTAT 
ATATTTGCCC ATGTTGGGCA GTTGACGGGC ATGTATCGAT ACAAATACAA GCTGATGCGA 
CAGATTCGCA TGTGCAAGGA CCTGAAGCAT CTCATCTATT ATCGTTTCAA CACGGGCCCT 
GTAGGGAAGG GTCCTGGCTG TGGCTTCTGG GCTGCCGGTT GGCGAGTCTG GCTCTTTTTC 
ATGCGTGGCA TTACCCCTTT ATTAGAGCGA TGGCTTGGCA ACCTCCTGGC CCGGCAGTTT 
GAAGGTCGAC ACTCAAAGGG GGTGGCAAAG ACAGTAACAA AGCAGCGAGT GGAGTCACAT 
TTTGACCTTG AGCTGCGGGC AGCTGTGATG CATGATATTC TGGACATGAT GCCTGAGGGG 
ATCAAGCAGA ACAAGGCCCG GACAATCCTG CAGCACCTCA GTGAAGCCTG GCGCTGCTGG 
AAAGCCAACA TTCCCTGGAA GGTCCCTGGG CTGCCGACGC CCATAGAGAA TATGATCCTT 
CGATACGTGA AGGCCAAGGC TGACTGGTGG ACCAACACTG CCCACTACAA CCGAGAACGG 
ATCCGCCGAG GGGCCACTGT GGACAAGACT GTTTGTAAAA AGAATCTGGG CCGCCTCACC 
CGGCTCTATC TGAAGGCAGA ACAGGAGCGG CAGCACAACT ACCTGAAGGA CGGGCCTTAC 
ATCACAGCGG AGGAAGCAGT GGCAGTATAT ACCACCACAG TGCATTGGTT GGAAAGCCGC 
AGGTTTTCAC CCATCCCATT CCCCCCACTC TCCTATAAGC ATGACACCAA GTTGCTCATC 
TTGGCATTGG AGCGGCTCAA GGAAGCTTAT AGTGTGAAGT CTCGGTTGAA CCAGTCTCAG 
AGGGAGGAGC TAGGTCTGAT CGAGCAGGCC TACGATAACC CCCACGAGGC GCTGTCCCGC 
ATCAAGCGTC ACCTCCTCAC ACAGAGAGCC TTCAAAGAGG TGGGCATTGA GTTCATGGAT 
CTGTATAGCC ACCTCGTTCC AGTATATGAT GTTGAGCCCC TGGAGAAGAT AACTGATGCT 
TACCTGGACC AGTACCTGTG GTATGAAGCC GACAAGCGCC GCCTGTTCCC ACCCTGGATT 
AAGCCTGCAG ACACAGAACC ACCTCCGCTG CTTGTTTACA AGTGGTGTCA AGGCATCAAT 
AACCTGCAGG ACGTGTGGGA GACGAGTGAA GGCGAGTGCA ATGTCATGCT GGAATCCCGC 
TTTGAGAAGA TGTATGAGAA GATCGACTTG ACTCTGCTCA ACAGGCTGCT GCGCCTCATC 
GTGGACCACA ACATAGCCGA CTACATGACA GCCAAGAACA ACGTCGTCAT CAACTATAAG 
GACATGAACC ATACGAATTC ATATGGGATC ATCAGAGGCC TGCAGTTTGC CTCATTCATC 
GTGCAGTATT ATGGCCTGGT GATGGATTTG CTTGTATTGG GATTGCACCG GGCCAGTGAG 
ATGGCTGGGC CCCCTCAGAT GCCAAATGAC TTTCTCAGTT TCCAGGACAT AGCCACTGAG 
GCTGCCCACC CCATCCGTCT CTTCTGCAGA TACATTGATC GCATCCATAT TTTTTTCAGG 
TTCACAGCAG ATGAGGCTCG GGACCTGATT CAACGTTACC TGACAGAGCA CCCTGACCCC 
AATAATGAAA ACATCGTTGG CTATAATAAC AAGAAGTGCT GGCCCCGAGA TGCCCGCATG 
CGCCTCATGA AACATGATGT TAACTTAGGC CGGGCGGTAT TCTGGGACAT CAAGAACCGC 
TTGCCACGGT CAGTGACTAC AGTTCAGTGG GAGAACAGCT TCGTGTCTGT GTACAGTAAG 
GACAACCCCA ACCTGCTGTT CAACATGTGT GGCTTCGAGT GCCGCATCCT GCCTAAGTGC 
CGCACCAGCT ATGAGGAGTT CACCCACAAG GACGGGGTCT GGAACCTGCA GAATGAGGTT 
ACTAAGGAGC GCACAGCTCA GTGTTTCCTG CGTGTGGACG ATGAGTCAAT GCAGCGCTTC 
CACAACCGCG TGCGTCAGAT TCTCATGGCC TCTGGGTCCA CCACCTTCAC CAAGATTGTG 
AATAAGTGGA ATACAGCTCT CATTGGCCTT ATGACATACT TTCGGGAGGC TGTGGTGAAC 
ACCCAAGAGC TCTTGGACTT ACTGGTGAAG TGTGAGAACA AAATCCAGAC ACGTATCAAG 
ATTGGACTCA ACTCCAAGAT GCCAAGTCGG TTCCCCCCGG TTGTGTTCTA CACCCCTAAG 
GAGTTGGGTG GACTCGGCAT GCTCTCAATG GGCCATGTGC TCATCCCCCA ATCCGACCTC 
AGGTGGTCCA AACAGACAGA TGTAGGTATC ACACACTTTC GTTCAGGAAT GAGCCATGAA 
GAAGACCAGC TCATTCCCAA CTTGTACCGC TACATACAGC CATGGGAGAG CGAGTTCATT 
GATTCTCAGC GGGTCTGGGC TGAGTACGCA CTCAAGAGGC AAGAGGCCAT TGCTCAGAAC 
AGACGCCTGA CTTTAGAAGA CCTAGAAGAT TCATGGGATC GTGGCATTCC TCGAATCAAT 
ACCCTCTTCC AGAAGGACCG GCACACACTG GCTTATGATA AGGGCTGGCG TGTCAGAACT 
GACTTTAAGC AGTATCAGGT TTTGAAGCAG AATCCGTTCT GGTGGACACA CCAGCGGCAT 
GATGGGAAGC TCTGGAACCT GAACAACTAC CGTACAGACA TGATCCAGGC CCTGGGCGGT 
GTGGAAGGCA TTCTGGAACA CACACTCTTT AAGGGCACTT ACTTCCCTAC CTGGGAGGGG 
CTTTTCTGGG AGAAGGCCAG TGGCTTTGAG GAATCTATGA AGTGGAAGAA GCTAACTAAT 
GCTCAGCGAT CAGGACTGAA CCAGATTCCC AATCGTAGAT TCACCCTCTG GTGGTCCCCG 
ACCATTAATC GAGCCAATGT ATATGTAGGC TTTCAGGTGC AGCTAGACCT GACGGGTATC 
TTCATGCACG GCAAGATCCC CACGCTGAAG ATCTCTCTCA TCCAGATCTT CCGAGCTCAC 
TTGTGGCAGA AGATCCATGA GAGCATTGTT ATGGACTTAT GTCAGGTGTT TGACCAGGAA 
CTTGATGCAC TGGAAATTGA GACAGTACAA AAGGAGACAA TCCATCCCCG AAAGTCATAT 
AAGATGAACT CTTCCTGTGC AGATATCCTG CTCTTTGCCT CCTATAAGTG GAATGTCTCC 
CGGCCCTCAT TGCTGGCTGA CTCCAAGGAT GTGATGGACA GCACCACCAC CCAGAAATAC 
TGGATTGACA TCCAGTTGCG CTGGGGGGAC TATGATTCCC ACGACATTGA GCGCTACGCC 
CGGGCCAAGT TCCTGGACTA CACCACCGAC AACATGAGTA TCTACCCTTC GCCCACAGGT 
GTACTCATCG CCATTGACCT GGCCTATAAC TTGCACAGTG CCTATGGAAA CTGGTTCCCA 
GGCAGCAAGC CTCTCATACA ACAGGCCATG GCCAAGATCA TGAAGGCAAA CCCTGCCCTG 
TATGTGTTAC GTGAACGGAT CCGCAAGGGG CTACAGCTCT ATTCATCTGA ACCCACTGAG 
CCTTATTTGT CTTCTCAGAA CTATGGTGAG CTCTTCTCCA ACCAGATTAT CTGGTTTGTG 
GATGACACCA ACGTCTACAG AGTGACTATT CACAAGACCT TTGAAGGGAA CTTGACAACC 
AAGCCCATCA ACGGAGCCAT CTTCATCTTC AACCCACGCA CAGGGCAGCT GTTCCTCAAG 
ATAATCCACA CGTCCGTGTG GGCGGGACAG AAGCGTTTGG GGCAGTTGGC TAAGTGGAAG 
ACAGCTGAGG AGGTGGCCGC CCTGATCCGA TCTCTGCCTG TGGAGGAGCA GCCCAAGCAG 
ATCATTGTCA CCAGGAAGGG CATGCTGGAC CCACTGGAGG TGCACTTACT GGACTTCCCC 
AATATTGTCA TCAAAGGATC GGAGCTCCAA CTCCCTTTCC AGGCGTGTCT CAAGGTGGAA 
AAATTCGGGG ATCTCATCCT TAAAGCCACT GAGCCCCAGA TGGTTCTCTT CAACCTCTAT 
GACGACTGGC TCAAGACTAT TTCATCTTAC ACGGCCTTCT CCCGTCTCAT CCTGATTCTG 
CGTGCCCTAC ATGTGAACAA CGATCGGGCA AAAGTGATCC TGAAGCCAGA CAAGACTACT 
ATTACAGAAC CACACCACAT CTGGCCCACT CTGACTGACG AAGAATGGAT CAAGGTCGAG 
GTGCAGCTCA AGGATCTGAT CTTGGCTGAC TACGGCAAGA AAAACAATGT GAACGTGGCA 
TCACTGACAC AATCAGAAAT TCGAGACATC ATCCTGGGTA TGGAGATCTC GGCACCGTCA 
CAGCAGCGGC AGCAGATCGC TGAGATCGAG AAGCAGACCA AGGAACAATC GCAGCTGACG 
GCAACACAGA CTCGCACTGT CAACAAGCAT GGCGATGAGA TCATCACCTC CACCACCAGC 
AACTATGAGA CCCAGACTTT CTCATCCAAG ACTGAGTGGA GGGTCAGGGC CATCTCTGCT 
GCCAACCTGC ACCTAAGGAC CAATCACATC TATGTTTCAT CTGACGACAT CAAGGAGACT 
GGCTACACCT ACATCCTTCC CAAGAATGTG CTTAAGAAGT TCATCTGCAT ATCTGACCTT 
CGGGCCCAAA TTGCAGGATA CCTATATGGG GTGAGCCCAC CAGATAACCC CCAGGTGAAG 
GAGATCCGCT GCATTGTGAT GGTGCCGCAG TGGGGCACTC ACCAGACCGT GCACCTGCCT 
GGCCAGCTGC CCCAGCATGA GTACCTCAAG GAGATGGAAC CCTTAGGTTG GATCCACACT 
CAGCCCAATG AGTCCCCGCA GTTATCACCC CAGGATGTCA CCACCCATGC CAAGATCATG 
GCTGACAACC CATCTTGGGA TGGCGAGAAG ACCATTATCA TCACATGCAG CTTCACGCCA 
GGCTCCTGTA CACTGACGGC CTACAAGCTG ACCCCCAGTG GCTACGAATG GGGCCGCCAG 
AACACAGACA AGGGCAACAA CCCCAAGGGC TACCTGCCTT CACACTATGA GAGGGTGCAG 
ATGCTGCTGT CGGACCGTTT CCTTGGCTTC TTCATGGTCC CTGCCCAGTC CTCGTGGAAC 
TACAACTTCA TGGGTGTTCG GCATGACCCC AACATGAAAT ATGAGCTACA GCTGGCGAAC 
CCCAAAGAGT TCTACCACGA GGTGCACAGG CCCTCTCACT TCCTCAACTT TGCTCTCCTG 
CAGGAGGGGG AGGTTTACTC TGCGGATCGG GAGGACCTGT ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of a human catalytic step I spliceosome.
Science (New York, N.Y.)(2018 Jan)
Zhan X,Yan C,Zhang X,Lei J,Shi Y

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PRPF8 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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