CACNA1E cDNA ORF clone, Homo sapiens(human)

The following CACNA1E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu10572 NM_000721.4
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
OHu10868 NM_001205294.2
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1091.30
$1559.00
OHu11422 NM_001205293.3
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1091.30
$1559.00
OHu90473 XM_017002243.1
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu90473 XM_017002251.1
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu90474 XM_017002244.1
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu90475 XM_017002245.1
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu90476 XM_017002246.1
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu90477 XM_017002247.1
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu90478 XM_017002248.1
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu90479 XM_017002249.1
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu90480 XM_017002250.1
Latest version!
Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
View Old Accession Versions >>
OHu10868 NM_001205294.1 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1091.30
$1559.00
OHu11422 NM_001205293.1 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1091.30
$1559.00
OHu10572 NM_000721.3 Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $923.30
$1319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu10572
    Clone ID Related Accession (Same CDS sequence) NM_000721.4 , NM_000721.3
    Accession Version NM_000721.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160059.7, BP230397.1, L29385.1, AK309070.1, AB209499.1, AL161734.12, BC037800.1 and KF510705.1. On May 31, 2019 this sequence version replaced NM_000721.3. Transcript Variant: This variant (3) is missing an in-frame coding exon compared to variant 1. This results in a shorter isoform (3, also known as alpha-1E-3) missing an internal protein segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L29385.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG
    CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT
    GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG
    TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC
    AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC
    GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT
    CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG
    GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG
    ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG
    AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA
    AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA
    GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA
    CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC
    AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG
    ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC
    TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC
    AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC
    AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG
    AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA
    GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT
    GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC
    ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT
    ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA
    GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCGATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCCGT GTGGGAGCAG
    CGTACCAGCC AGCTGAGGAA GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAGAGAG
    GAGGCGCCGA CCATGAACCC GCTCAACCCC CTCAACCCGC TCAGCTCCCT CAACCCGCTC
    AATGCCCACC CCAGCCTTTA TCGGCGACCC AGGGCCATTG AGGGCCTGGC CCTGGGCCTG
    GCCCTGGAGA AGTTCGAGGA GGAGCGCATC AGCCGTGGGG GGTCCCTCAA GGGGGATGGA
    GGGGACCGAT CCAGTGCCCT GGACAACCAG AGGACCCCTT TGTCCCTGGG CCAGCGGGAG
    CCACCATGGC TGGCCAGGCC CTGTCATGGA AACTGTGACC CGACTCAGCA GGAGGCAGGG
    GGAGGAGAGG CTGTGGTGAC CTTTGAGGAC CGGGCCAGGC ACAGGCAGAG CCAACGGCGC
    AGCCGGCATC GCCGCGTCAG GACAGAAGGC AAGGAGTCCT CTTCAGCCTC CCGGAGCAGG
    TCTGCCAGCC AGGAACGCAG TCTGGATGAA GCCATGCCCA CTGAAGGGGA GAAGGACCAT
    GAGCTCAGGG GCAACCATGG TGCCAAGGAG CCAACGATCC AAGAAGAGAG AGCCCAGGAT
    TTAAGGAGGA CCAACAGTCT GATGGTGTCC AGAGGCTCCG GGCTGGCAGG AGGCCTTGAT
    GAGGCTGACA CCCCCCTAGT CCTGCCCCAT CCTGAGCTGG AAGTGGGGAA GCACGTGGTG
    CTGACGGAGC AGGAGCCAGA AGGCAGCAGT GAGCAGGCCC TGCTGGGGAA TGTGCAGCTA
    GACATGGGCC GGGTCATCAG CCAGAGCGAG CCTGACCTCT CCTGCATCAC GGCCAACACG
    GACAAGGCCA CCACCGAGAG CACCAGCGTC ACCGTCGCCA TCCCCGACGT GGACCCCTTG
    GTGGACTCAA CCGTGGTGCA CATTAGCAAC AAGACGGATG GGGAAGCCAG TCCCTTGAAG
    GAGGCAGAGA TCAGAGAGGA TGAGGAGGAG GTGGAGAAGA AGAAGCAGAA GAAGGAGAAG
    CGTGAGACAG GCAAAGCCAT GGTGCCCCAC AGCTCAATGT TCATCTTCAG CACCACCAAC
    CCGATCCGGA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATCCTC
    CTGGTGATTG CAGCCAGCAG CATCGCCCTG GCGGCAGAGG ACCCCGTCCT GACCAACTCG
    GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTGTTCA CGGGCGTGTT CACCTTTGAG
    ATGGTTATAA AGATGATAGA CCAAGGCTTG ATCCTGCAGG ATGGGTCCTA CTTCCGAGAC
    TTGTGGAACA TCCTGGACTT TGTGGTGGTC GTTGGCGCAT TGGTGGCCTT TGCTCTGGCG
    AACGCTTTGG GAACCAACAA AGGACGGGAC ATCAAGACCA TCAAGTCTCT GCGGGTGCTC
    CGAGTTCTAA GGCCACTGAA AACCATCAAG CGCTTGCCCA AGCTCAAGGC CGTCTTCGAC
    TGCGTAGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TTGTGTACAA GCTCTTCATG
    TTCATCTTTG CTGTCATCGC AGTTCAGCTC TTCAAGGGAA AGTTCTTTTA TTGCACGGAC
    AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTATG TAGATCATGA GAAAAACAAG
    ATGGAGGTGA AGGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG
    GCCCTGCTGA CCCTCTTCAC CGTCTCCACA GGGGAAGGAT GGCCTCAAGT TCTGCAGCAC
    TCTGTAGATG TGACAGAGGA AGACCGAGGC CCAAGCCGCA GCAACCGCAT GGAGATGTCT
    ATCTTTTATG TAGTCTACTT TGTGGTCTTC CCCTTCTTCT TTGTCAATAT CTTTGTGGCT
    CTCATCATCA TCACCTTCCA GGAGCAAGGG GATAAGATGA TGGAGGAGTG CAGCCTGGAG
    AAGAATGAGA GGGCGTGCAT CGACTTCGCC ATCAGCGCCA AACCTCTCAC CCGCTACATG
    CCGCAGAACA GACACACCTT CCAGTACCGC GTGTGGCACT TTGTGGTGTC TCCGTCCTTT
    GAGTACACCA TTATGGCCAT GATCGCCTTG AATACTGTTG TGCTGATGAT GAAGTATTAT
    TCTGCTCCCT GTACCTATGA GCTGGCCCTG AAGTACCTGA ATATCGCCTT CACCATGGTG
    TTTTCCCTGG AATGTGTCCT GAAGGTCATC GCTTTTGGCT TTTTGAACTA TTTCCGAGAC
    ACCTGGAATA TCTTTGACTT CATCACCGTG ATTGGCAGTA TCACAGAAAT TATCCTGACA
    GACAGCAAGC TGGTGAACAC CAGTGGCTTC AATATGAGCT TTCTGAAGCT CTTCCGAGCT
    GCCCGCCTCA TAAAGCTCCT GCGTCAGGGC TATACCATAC GCATTTTGCT GTGGACCTTT
    GTGCAGTCCT TTAAGGCCCT CCCTTATGTC TGCCTTTTAA TTGCCATGCT TTTCTTCATT
    TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGACGAGGA GAGTCACATC
    AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTAA TGCTACTCTT CAGGAGTGCC
    ACAGGTGAGG CCTGGCAGGA GATTATGCTG TCATGCCTTG GGGAGAAGGG CTGTGAGCCT
    GACACCACCG CACCATCAGG GCAGAACGAG AACGAACGCT GCGGCACCGA TCTGGCCTAC
    GTGTACTTTG TCTCCTTCAT CTTCTTCTGC TCCTTCTTGA TGCTCAACCT GTTTGTGGCC
    GTCATCATGG ACAACTTTGA GTACCTGACT CGGGACTCCT CCATCCTGGG GCCTCACCAC
    TTGGACGAGT TTGTCCGCGT CTGGGCAGAA TATGACCGAG CAGCATGTGG CCGCATCCAT
    TACACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCTC CGCTAGGCCT CGGCAAGAGA
    TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTGATGA ACATGCCAGT AGCTGAGGAC
    ATGACGGTCC ACTTCACCTC CACACTTATG GCTCTGATCC GGACAGCTCT GGACATTAAA
    ATTGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCAG AGCTACAAAA GGAGACCCTA
    GCCATCTGGC CTCACCTATC CCAGAAGATG CTGGATCTGC TTGTGCCCAT GCCCAAAGCC
    TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAGCAG
    AGTAAGGTGA AGAAGCAGAG GCAGCAGCTG GAGGAACAGA AAAATGCCCC CATGTTCCAG
    CGCATGGAGC CTTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCTTAC
    CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGGAGTG GATACCCTTC GATGAGTCCA
    CTCTCTCCCC AGGATATATT CCAGTTGGCT TGTATGGACC CCGCCGATGA CGGACAGTTC
    CAAGAACGGC AGTCTCTGGT GGTGACAGAC CCTAGCTCCA TGAGACGTTC ATTTTCCACT
    ATTCGGGATA AGCGTTCAAA TTCCTCGTGG TTGGAGGAAT TCTCCATGGA GCGAAGCAGT
    GAAAATACCT ACAAGTCCCG TCGCCGGAGT TACCACTCCT CCTTGCGGCT GTCAGCCCAC
    CGCCTGAACT CTGATTCAGG CCACAAGTCT GACACTCACC GCTCAGGGGG CAGGGAGCGG
    GGACGATCAA AAGAGCGAAA GCATCTTCTC TCTCCTGATG TCTCCCGCTG CAATTCAGAA
    GAGCGAGGGA CCCAGGCTGA CTGGGAGTCC CCAGAGCGCC GTCAATCCAG GTCACCCAGT
    GAGGGCAGGT CACAGACGCC CAACAGACAG GGCACAGGTT CCCTAAGTGA GAGCTCCATC
    CCCTCTGTCT CTGACACCAG CACCCCAAGA AGAAGTCGTC GGCAGCTCCC ACCCGTCCCG
    CCAAAGCCCC GGCCCCTCCT TTCCTACAGC TCCCTGATTC GACACGCGGG CAGCATCTCT
    CCACCTGCTG ATGGAAGCGA GGAGGGCTCC CCGCTGACCT CCCAAGCTCT GGAGAGCAAC
    AATGCTTGCC TGACCGAGTC TTCCAACTCT CCGCACCCCC AGCAGAGCCA ACATGCCTCC
    CCACAGCGCT ACATCTCCGA GCCCTACTTG GCCCTGCACG AAGACTCCCA CGCCTCAGAC
    TGTGGTGAGG AGGAGACGCT CACTTTCGAA GCAGCCGTGG CTACTAGCCT GGGCCGTTCC
    AACACCATCG GCTCAGCCCC ACCCCTGCGG CATAGCTGGC AGATGCCCAA CGGGCACTAT
    CGGCGGCGGA GGCGCGGGGG GCCTGGGCCA GGCATGATGT GTGGGGCTGT CAACAACCTG
    CTAAGTGACA CGGAAGAAGA TGACAAATGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000712.2
    CDS229..7041
    Translation

    Target ORF information:

    RefSeq Version NM_000721.4
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000721.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG
    CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT
    GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG
    TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC
    AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC
    GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT
    CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG
    GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG
    ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG
    AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA
    AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA
    GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA
    CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC
    AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG
    ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC
    TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC
    AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC
    AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG
    AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA
    GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT
    GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC
    ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT
    ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA
    GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCGATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCCGT GTGGGAGCAG
    CGTACCAGCC AGCTGAGGAA GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAGAGAG
    GAGGCGCCGA CCATGAACCC GCTCAACCCC CTCAACCCGC TCAGCTCCCT CAACCCGCTC
    AATGCCCACC CCAGCCTTTA TCGGCGACCC AGGGCCATTG AGGGCCTGGC CCTGGGCCTG
    GCCCTGGAGA AGTTCGAGGA GGAGCGCATC AGCCGTGGGG GGTCCCTCAA GGGGGATGGA
    GGGGACCGAT CCAGTGCCCT GGACAACCAG AGGACCCCTT TGTCCCTGGG CCAGCGGGAG
    CCACCATGGC TGGCCAGGCC CTGTCATGGA AACTGTGACC CGACTCAGCA GGAGGCAGGG
    GGAGGAGAGG CTGTGGTGAC CTTTGAGGAC CGGGCCAGGC ACAGGCAGAG CCAACGGCGC
    AGCCGGCATC GCCGCGTCAG GACAGAAGGC AAGGAGTCCT CTTCAGCCTC CCGGAGCAGG
    TCTGCCAGCC AGGAACGCAG TCTGGATGAA GCCATGCCCA CTGAAGGGGA GAAGGACCAT
    GAGCTCAGGG GCAACCATGG TGCCAAGGAG CCAACGATCC AAGAAGAGAG AGCCCAGGAT
    TTAAGGAGGA CCAACAGTCT GATGGTGTCC AGAGGCTCCG GGCTGGCAGG AGGCCTTGAT
    GAGGCTGACA CCCCCCTAGT CCTGCCCCAT CCTGAGCTGG AAGTGGGGAA GCACGTGGTG
    CTGACGGAGC AGGAGCCAGA AGGCAGCAGT GAGCAGGCCC TGCTGGGGAA TGTGCAGCTA
    GACATGGGCC GGGTCATCAG CCAGAGCGAG CCTGACCTCT CCTGCATCAC GGCCAACACG
    GACAAGGCCA CCACCGAGAG CACCAGCGTC ACCGTCGCCA TCCCCGACGT GGACCCCTTG
    GTGGACTCAA CCGTGGTGCA CATTAGCAAC AAGACGGATG GGGAAGCCAG TCCCTTGAAG
    GAGGCAGAGA TCAGAGAGGA TGAGGAGGAG GTGGAGAAGA AGAAGCAGAA GAAGGAGAAG
    CGTGAGACAG GCAAAGCCAT GGTGCCCCAC AGCTCAATGT TCATCTTCAG CACCACCAAC
    CCGATCCGGA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATCCTC
    CTGGTGATTG CAGCCAGCAG CATCGCCCTG GCGGCAGAGG ACCCCGTCCT GACCAACTCG
    GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTGTTCA CGGGCGTGTT CACCTTTGAG
    ATGGTTATAA AGATGATAGA CCAAGGCTTG ATCCTGCAGG ATGGGTCCTA CTTCCGAGAC
    TTGTGGAACA TCCTGGACTT TGTGGTGGTC GTTGGCGCAT TGGTGGCCTT TGCTCTGGCG
    AACGCTTTGG GAACCAACAA AGGACGGGAC ATCAAGACCA TCAAGTCTCT GCGGGTGCTC
    CGAGTTCTAA GGCCACTGAA AACCATCAAG CGCTTGCCCA AGCTCAAGGC CGTCTTCGAC
    TGCGTAGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TTGTGTACAA GCTCTTCATG
    TTCATCTTTG CTGTCATCGC AGTTCAGCTC TTCAAGGGAA AGTTCTTTTA TTGCACGGAC
    AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTATG TAGATCATGA GAAAAACAAG
    ATGGAGGTGA AGGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG
    GCCCTGCTGA CCCTCTTCAC CGTCTCCACA GGGGAAGGAT GGCCTCAAGT TCTGCAGCAC
    TCTGTAGATG TGACAGAGGA AGACCGAGGC CCAAGCCGCA GCAACCGCAT GGAGATGTCT
    ATCTTTTATG TAGTCTACTT TGTGGTCTTC CCCTTCTTCT TTGTCAATAT CTTTGTGGCT
    CTCATCATCA TCACCTTCCA GGAGCAAGGG GATAAGATGA TGGAGGAGTG CAGCCTGGAG
    AAGAATGAGA GGGCGTGCAT CGACTTCGCC ATCAGCGCCA AACCTCTCAC CCGCTACATG
    CCGCAGAACA GACACACCTT CCAGTACCGC GTGTGGCACT TTGTGGTGTC TCCGTCCTTT
    GAGTACACCA TTATGGCCAT GATCGCCTTG AATACTGTTG TGCTGATGAT GAAGTATTAT
    TCTGCTCCCT GTACCTATGA GCTGGCCCTG AAGTACCTGA ATATCGCCTT CACCATGGTG
    TTTTCCCTGG AATGTGTCCT GAAGGTCATC GCTTTTGGCT TTTTGAACTA TTTCCGAGAC
    ACCTGGAATA TCTTTGACTT CATCACCGTG ATTGGCAGTA TCACAGAAAT TATCCTGACA
    GACAGCAAGC TGGTGAACAC CAGTGGCTTC AATATGAGCT TTCTGAAGCT CTTCCGAGCT
    GCCCGCCTCA TAAAGCTCCT GCGTCAGGGC TATACCATAC GCATTTTGCT GTGGACCTTT
    GTGCAGTCCT TTAAGGCCCT CCCTTATGTC TGCCTTTTAA TTGCCATGCT TTTCTTCATT
    TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGACGAGGA GAGTCACATC
    AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTAA TGCTACTCTT CAGGAGTGCC
    ACAGGTGAGG CCTGGCAGGA GATTATGCTG TCATGCCTTG GGGAGAAGGG CTGTGAGCCT
    GACACCACCG CACCATCAGG GCAGAACGAG AACGAACGCT GCGGCACCGA TCTGGCCTAC
    GTGTACTTTG TCTCCTTCAT CTTCTTCTGC TCCTTCTTGA TGCTCAACCT GTTTGTGGCC
    GTCATCATGG ACAACTTTGA GTACCTGACT CGGGACTCCT CCATCCTGGG GCCTCACCAC
    TTGGACGAGT TTGTCCGCGT CTGGGCAGAA TATGACCGAG CAGCATGTGG CCGCATCCAT
    TACACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCTC CGCTAGGCCT CGGCAAGAGA
    TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTGATGA ACATGCCAGT AGCTGAGGAC
    ATGACGGTCC ACTTCACCTC CACACTTATG GCTCTGATCC GGACAGCTCT GGACATTAAA
    ATTGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCAG AGCTACAAAA GGAGACCCTA
    GCCATCTGGC CTCACCTATC CCAGAAGATG CTGGATCTGC TTGTGCCCAT GCCCAAAGCC
    TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAGCAG
    AGTAAGGTGA AGAAGCAGAG GCAGCAGCTG GAGGAACAGA AAAATGCCCC CATGTTCCAG
    CGCATGGAGC CTTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCTTAC
    CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGGAGTG GATACCCTTC GATGAGTCCA
    CTCTCTCCCC AGGATATATT CCAGTTGGCT TGTATGGACC CCGCCGATGA CGGACAGTTC
    CAAGAACGGC AGTCTCTGGT GGTGACAGAC CCTAGCTCCA TGAGACGTTC ATTTTCCACT
    ATTCGGGATA AGCGTTCAAA TTCCTCGTGG TTGGAGGAAT TCTCCATGGA GCGAAGCAGT
    GAAAATACCT ACAAGTCCCG TCGCCGGAGT TACCACTCCT CCTTGCGGCT GTCAGCCCAC
    CGCCTGAACT CTGATTCAGG CCACAAGTCT GACACTCACC GCTCAGGGGG CAGGGAGCGG
    GGACGATCAA AAGAGCGAAA GCATCTTCTC TCTCCTGATG TCTCCCGCTG CAATTCAGAA
    GAGCGAGGGA CCCAGGCTGA CTGGGAGTCC CCAGAGCGCC GTCAATCCAG GTCACCCAGT
    GAGGGCAGGT CACAGACGCC CAACAGACAG GGCACAGGTT CCCTAAGTGA GAGCTCCATC
    CCCTCTGTCT CTGACACCAG CACCCCAAGA AGAAGTCGTC GGCAGCTCCC ACCCGTCCCG
    CCAAAGCCCC GGCCCCTCCT TTCCTACAGC TCCCTGATTC GACACGCGGG CAGCATCTCT
    CCACCTGCTG ATGGAAGCGA GGAGGGCTCC CCGCTGACCT CCCAAGCTCT GGAGAGCAAC
    AATGCTTGCC TGACCGAGTC TTCCAACTCT CCGCACCCCC AGCAGAGCCA ACATGCCTCC
    CCACAGCGCT ACATCTCCGA GCCCTACTTG GCCCTGCACG AAGACTCCCA CGCCTCAGAC
    TGTGGTGAGG AGGAGACGCT CACTTTCGAA GCAGCCGTGG CTACTAGCCT GGGCCGTTCC
    AACACCATCG GCTCAGCCCC ACCCCTGCGG CATAGCTGGC AGATGCCCAA CGGGCACTAT
    CGGCGGCGGA GGCGCGGGGG GCCTGGGCCA GGCATGATGT GTGGGGCTGT CAACAACCTG
    CTAAGTGACA CGGAAGAAGA TGACAAATGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10572
    Clone ID Related Accession (Same CDS sequence) NM_000721.4 , NM_000721.3
    Accession Version NM_000721.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP230397.1, L29385.1, AK309070.1, AB209499.1, AL161734.12 and BC037800.1. On Apr 20, 2011 this sequence version replaced NM_000721.2. Transcript Variant: This variant (3) is missing an in-frame coding exon compared to variant 1. This results in a shorter isoform (3, also known as alpha-1E-3) missing an internal protein segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L29385.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG
    CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT
    GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG
    TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC
    AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC
    GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT
    CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG
    GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG
    ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG
    AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA
    AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA
    GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA
    CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC
    AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG
    ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC
    TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC
    AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC
    AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG
    AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA
    GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT
    GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC
    ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT
    ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA
    GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCGATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCCGT GTGGGAGCAG
    CGTACCAGCC AGCTGAGGAA GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAGAGAG
    GAGGCGCCGA CCATGAACCC GCTCAACCCC CTCAACCCGC TCAGCTCCCT CAACCCGCTC
    AATGCCCACC CCAGCCTTTA TCGGCGACCC AGGGCCATTG AGGGCCTGGC CCTGGGCCTG
    GCCCTGGAGA AGTTCGAGGA GGAGCGCATC AGCCGTGGGG GGTCCCTCAA GGGGGATGGA
    GGGGACCGAT CCAGTGCCCT GGACAACCAG AGGACCCCTT TGTCCCTGGG CCAGCGGGAG
    CCACCATGGC TGGCCAGGCC CTGTCATGGA AACTGTGACC CGACTCAGCA GGAGGCAGGG
    GGAGGAGAGG CTGTGGTGAC CTTTGAGGAC CGGGCCAGGC ACAGGCAGAG CCAACGGCGC
    AGCCGGCATC GCCGCGTCAG GACAGAAGGC AAGGAGTCCT CTTCAGCCTC CCGGAGCAGG
    TCTGCCAGCC AGGAACGCAG TCTGGATGAA GCCATGCCCA CTGAAGGGGA GAAGGACCAT
    GAGCTCAGGG GCAACCATGG TGCCAAGGAG CCAACGATCC AAGAAGAGAG AGCCCAGGAT
    TTAAGGAGGA CCAACAGTCT GATGGTGTCC AGAGGCTCCG GGCTGGCAGG AGGCCTTGAT
    GAGGCTGACA CCCCCCTAGT CCTGCCCCAT CCTGAGCTGG AAGTGGGGAA GCACGTGGTG
    CTGACGGAGC AGGAGCCAGA AGGCAGCAGT GAGCAGGCCC TGCTGGGGAA TGTGCAGCTA
    GACATGGGCC GGGTCATCAG CCAGAGCGAG CCTGACCTCT CCTGCATCAC GGCCAACACG
    GACAAGGCCA CCACCGAGAG CACCAGCGTC ACCGTCGCCA TCCCCGACGT GGACCCCTTG
    GTGGACTCAA CCGTGGTGCA CATTAGCAAC AAGACGGATG GGGAAGCCAG TCCCTTGAAG
    GAGGCAGAGA TCAGAGAGGA TGAGGAGGAG GTGGAGAAGA AGAAGCAGAA GAAGGAGAAG
    CGTGAGACAG GCAAAGCCAT GGTGCCCCAC AGCTCAATGT TCATCTTCAG CACCACCAAC
    CCGATCCGGA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATCCTC
    CTGGTGATTG CAGCCAGCAG CATCGCCCTG GCGGCAGAGG ACCCCGTCCT GACCAACTCG
    GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTGTTCA CGGGCGTGTT CACCTTTGAG
    ATGGTTATAA AGATGATAGA CCAAGGCTTG ATCCTGCAGG ATGGGTCCTA CTTCCGAGAC
    TTGTGGAACA TCCTGGACTT TGTGGTGGTC GTTGGCGCAT TGGTGGCCTT TGCTCTGGCG
    AACGCTTTGG GAACCAACAA AGGACGGGAC ATCAAGACCA TCAAGTCTCT GCGGGTGCTC
    CGAGTTCTAA GGCCACTGAA AACCATCAAG CGCTTGCCCA AGCTCAAGGC CGTCTTCGAC
    TGCGTAGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TTGTGTACAA GCTCTTCATG
    TTCATCTTTG CTGTCATCGC AGTTCAGCTC TTCAAGGGAA AGTTCTTTTA TTGCACGGAC
    AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTATG TAGATCATGA GAAAAACAAG
    ATGGAGGTGA AGGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG
    GCCCTGCTGA CCCTCTTCAC CGTCTCCACA GGGGAAGGAT GGCCTCAAGT TCTGCAGCAC
    TCTGTAGATG TGACAGAGGA AGACCGAGGC CCAAGCCGCA GCAACCGCAT GGAGATGTCT
    ATCTTTTATG TAGTCTACTT TGTGGTCTTC CCCTTCTTCT TTGTCAATAT CTTTGTGGCT
    CTCATCATCA TCACCTTCCA GGAGCAAGGG GATAAGATGA TGGAGGAGTG CAGCCTGGAG
    AAGAATGAGA GGGCGTGCAT CGACTTCGCC ATCAGCGCCA AACCTCTCAC CCGCTACATG
    CCGCAGAACA GACACACCTT CCAGTACCGC GTGTGGCACT TTGTGGTGTC TCCGTCCTTT
    GAGTACACCA TTATGGCCAT GATCGCCTTG AATACTGTTG TGCTGATGAT GAAGTATTAT
    TCTGCTCCCT GTACCTATGA GCTGGCCCTG AAGTACCTGA ATATCGCCTT CACCATGGTG
    TTTTCCCTGG AATGTGTCCT GAAGGTCATC GCTTTTGGCT TTTTGAACTA TTTCCGAGAC
    ACCTGGAATA TCTTTGACTT CATCACCGTG ATTGGCAGTA TCACAGAAAT TATCCTGACA
    GACAGCAAGC TGGTGAACAC CAGTGGCTTC AATATGAGCT TTCTGAAGCT CTTCCGAGCT
    GCCCGCCTCA TAAAGCTCCT GCGTCAGGGC TATACCATAC GCATTTTGCT GTGGACCTTT
    GTGCAGTCCT TTAAGGCCCT CCCTTATGTC TGCCTTTTAA TTGCCATGCT TTTCTTCATT
    TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGACGAGGA GAGTCACATC
    AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTAA TGCTACTCTT CAGGAGTGCC
    ACAGGTGAGG CCTGGCAGGA GATTATGCTG TCATGCCTTG GGGAGAAGGG CTGTGAGCCT
    GACACCACCG CACCATCAGG GCAGAACGAG AACGAACGCT GCGGCACCGA TCTGGCCTAC
    GTGTACTTTG TCTCCTTCAT CTTCTTCTGC TCCTTCTTGA TGCTCAACCT GTTTGTGGCC
    GTCATCATGG ACAACTTTGA GTACCTGACT CGGGACTCCT CCATCCTGGG GCCTCACCAC
    TTGGACGAGT TTGTCCGCGT CTGGGCAGAA TATGACCGAG CAGCATGTGG CCGCATCCAT
    TACACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCTC CGCTAGGCCT CGGCAAGAGA
    TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTGATGA ACATGCCAGT AGCTGAGGAC
    ATGACGGTCC ACTTCACCTC CACACTTATG GCTCTGATCC GGACAGCTCT GGACATTAAA
    ATTGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCAG AGCTACAAAA GGAGACCCTA
    GCCATCTGGC CTCACCTATC CCAGAAGATG CTGGATCTGC TTGTGCCCAT GCCCAAAGCC
    TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAGCAG
    AGTAAGGTGA AGAAGCAGAG GCAGCAGCTG GAGGAACAGA AAAATGCCCC CATGTTCCAG
    CGCATGGAGC CTTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCTTAC
    CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGGAGTG GATACCCTTC GATGAGTCCA
    CTCTCTCCCC AGGATATATT CCAGTTGGCT TGTATGGACC CCGCCGATGA CGGACAGTTC
    CAAGAACGGC AGTCTCTGGT GGTGACAGAC CCTAGCTCCA TGAGACGTTC ATTTTCCACT
    ATTCGGGATA AGCGTTCAAA TTCCTCGTGG TTGGAGGAAT TCTCCATGGA GCGAAGCAGT
    GAAAATACCT ACAAGTCCCG TCGCCGGAGT TACCACTCCT CCTTGCGGCT GTCAGCCCAC
    CGCCTGAACT CTGATTCAGG CCACAAGTCT GACACTCACC GCTCAGGGGG CAGGGAGCGG
    GGACGATCAA AAGAGCGAAA GCATCTTCTC TCTCCTGATG TCTCCCGCTG CAATTCAGAA
    GAGCGAGGGA CCCAGGCTGA CTGGGAGTCC CCAGAGCGCC GTCAATCCAG GTCACCCAGT
    GAGGGCAGGT CACAGACGCC CAACAGACAG GGCACAGGTT CCCTAAGTGA GAGCTCCATC
    CCCTCTGTCT CTGACACCAG CACCCCAAGA AGAAGTCGTC GGCAGCTCCC ACCCGTCCCG
    CCAAAGCCCC GGCCCCTCCT TTCCTACAGC TCCCTGATTC GACACGCGGG CAGCATCTCT
    CCACCTGCTG ATGGAAGCGA GGAGGGCTCC CCGCTGACCT CCCAAGCTCT GGAGAGCAAC
    AATGCTTGCC TGACCGAGTC TTCCAACTCT CCGCACCCCC AGCAGAGCCA ACATGCCTCC
    CCACAGCGCT ACATCTCCGA GCCCTACTTG GCCCTGCACG AAGACTCCCA CGCCTCAGAC
    TGTGGTGAGG AGGAGACGCT CACTTTCGAA GCAGCCGTGG CTACTAGCCT GGGCCGTTCC
    AACACCATCG GCTCAGCCCC ACCCCTGCGG CATAGCTGGC AGATGCCCAA CGGGCACTAT
    CGGCGGCGGA GGCGCGGGGG GCCTGGGCCA GGCATGATGT GTGGGGCTGT CAACAACCTG
    CTAAGTGACA CGGAAGAAGA TGACAAATGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000712.2
    CDS196..7008
    Translation

    Target ORF information:

    RefSeq Version NM_000721.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000721.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG
    CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT
    GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG
    TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC
    AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC
    GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT
    CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG
    GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG
    ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG
    AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA
    AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA
    GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA
    CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC
    AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG
    ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC
    TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC
    AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC
    AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG
    AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA
    GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT
    GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC
    ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT
    ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA
    GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCGATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCCGT GTGGGAGCAG
    CGTACCAGCC AGCTGAGGAA GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAGAGAG
    GAGGCGCCGA CCATGAACCC GCTCAACCCC CTCAACCCGC TCAGCTCCCT CAACCCGCTC
    AATGCCCACC CCAGCCTTTA TCGGCGACCC AGGGCCATTG AGGGCCTGGC CCTGGGCCTG
    GCCCTGGAGA AGTTCGAGGA GGAGCGCATC AGCCGTGGGG GGTCCCTCAA GGGGGATGGA
    GGGGACCGAT CCAGTGCCCT GGACAACCAG AGGACCCCTT TGTCCCTGGG CCAGCGGGAG
    CCACCATGGC TGGCCAGGCC CTGTCATGGA AACTGTGACC CGACTCAGCA GGAGGCAGGG
    GGAGGAGAGG CTGTGGTGAC CTTTGAGGAC CGGGCCAGGC ACAGGCAGAG CCAACGGCGC
    AGCCGGCATC GCCGCGTCAG GACAGAAGGC AAGGAGTCCT CTTCAGCCTC CCGGAGCAGG
    TCTGCCAGCC AGGAACGCAG TCTGGATGAA GCCATGCCCA CTGAAGGGGA GAAGGACCAT
    GAGCTCAGGG GCAACCATGG TGCCAAGGAG CCAACGATCC AAGAAGAGAG AGCCCAGGAT
    TTAAGGAGGA CCAACAGTCT GATGGTGTCC AGAGGCTCCG GGCTGGCAGG AGGCCTTGAT
    GAGGCTGACA CCCCCCTAGT CCTGCCCCAT CCTGAGCTGG AAGTGGGGAA GCACGTGGTG
    CTGACGGAGC AGGAGCCAGA AGGCAGCAGT GAGCAGGCCC TGCTGGGGAA TGTGCAGCTA
    GACATGGGCC GGGTCATCAG CCAGAGCGAG CCTGACCTCT CCTGCATCAC GGCCAACACG
    GACAAGGCCA CCACCGAGAG CACCAGCGTC ACCGTCGCCA TCCCCGACGT GGACCCCTTG
    GTGGACTCAA CCGTGGTGCA CATTAGCAAC AAGACGGATG GGGAAGCCAG TCCCTTGAAG
    GAGGCAGAGA TCAGAGAGGA TGAGGAGGAG GTGGAGAAGA AGAAGCAGAA GAAGGAGAAG
    CGTGAGACAG GCAAAGCCAT GGTGCCCCAC AGCTCAATGT TCATCTTCAG CACCACCAAC
    CCGATCCGGA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATCCTC
    CTGGTGATTG CAGCCAGCAG CATCGCCCTG GCGGCAGAGG ACCCCGTCCT GACCAACTCG
    GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTGTTCA CGGGCGTGTT CACCTTTGAG
    ATGGTTATAA AGATGATAGA CCAAGGCTTG ATCCTGCAGG ATGGGTCCTA CTTCCGAGAC
    TTGTGGAACA TCCTGGACTT TGTGGTGGTC GTTGGCGCAT TGGTGGCCTT TGCTCTGGCG
    AACGCTTTGG GAACCAACAA AGGACGGGAC ATCAAGACCA TCAAGTCTCT GCGGGTGCTC
    CGAGTTCTAA GGCCACTGAA AACCATCAAG CGCTTGCCCA AGCTCAAGGC CGTCTTCGAC
    TGCGTAGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TTGTGTACAA GCTCTTCATG
    TTCATCTTTG CTGTCATCGC AGTTCAGCTC TTCAAGGGAA AGTTCTTTTA TTGCACGGAC
    AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTATG TAGATCATGA GAAAAACAAG
    ATGGAGGTGA AGGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG
    GCCCTGCTGA CCCTCTTCAC CGTCTCCACA GGGGAAGGAT GGCCTCAAGT TCTGCAGCAC
    TCTGTAGATG TGACAGAGGA AGACCGAGGC CCAAGCCGCA GCAACCGCAT GGAGATGTCT
    ATCTTTTATG TAGTCTACTT TGTGGTCTTC CCCTTCTTCT TTGTCAATAT CTTTGTGGCT
    CTCATCATCA TCACCTTCCA GGAGCAAGGG GATAAGATGA TGGAGGAGTG CAGCCTGGAG
    AAGAATGAGA GGGCGTGCAT CGACTTCGCC ATCAGCGCCA AACCTCTCAC CCGCTACATG
    CCGCAGAACA GACACACCTT CCAGTACCGC GTGTGGCACT TTGTGGTGTC TCCGTCCTTT
    GAGTACACCA TTATGGCCAT GATCGCCTTG AATACTGTTG TGCTGATGAT GAAGTATTAT
    TCTGCTCCCT GTACCTATGA GCTGGCCCTG AAGTACCTGA ATATCGCCTT CACCATGGTG
    TTTTCCCTGG AATGTGTCCT GAAGGTCATC GCTTTTGGCT TTTTGAACTA TTTCCGAGAC
    ACCTGGAATA TCTTTGACTT CATCACCGTG ATTGGCAGTA TCACAGAAAT TATCCTGACA
    GACAGCAAGC TGGTGAACAC CAGTGGCTTC AATATGAGCT TTCTGAAGCT CTTCCGAGCT
    GCCCGCCTCA TAAAGCTCCT GCGTCAGGGC TATACCATAC GCATTTTGCT GTGGACCTTT
    GTGCAGTCCT TTAAGGCCCT CCCTTATGTC TGCCTTTTAA TTGCCATGCT TTTCTTCATT
    TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGACGAGGA GAGTCACATC
    AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTAA TGCTACTCTT CAGGAGTGCC
    ACAGGTGAGG CCTGGCAGGA GATTATGCTG TCATGCCTTG GGGAGAAGGG CTGTGAGCCT
    GACACCACCG CACCATCAGG GCAGAACGAG AACGAACGCT GCGGCACCGA TCTGGCCTAC
    GTGTACTTTG TCTCCTTCAT CTTCTTCTGC TCCTTCTTGA TGCTCAACCT GTTTGTGGCC
    GTCATCATGG ACAACTTTGA GTACCTGACT CGGGACTCCT CCATCCTGGG GCCTCACCAC
    TTGGACGAGT TTGTCCGCGT CTGGGCAGAA TATGACCGAG CAGCATGTGG CCGCATCCAT
    TACACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCTC CGCTAGGCCT CGGCAAGAGA
    TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTGATGA ACATGCCAGT AGCTGAGGAC
    ATGACGGTCC ACTTCACCTC CACACTTATG GCTCTGATCC GGACAGCTCT GGACATTAAA
    ATTGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCAG AGCTACAAAA GGAGACCCTA
    GCCATCTGGC CTCACCTATC CCAGAAGATG CTGGATCTGC TTGTGCCCAT GCCCAAAGCC
    TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAGCAG
    AGTAAGGTGA AGAAGCAGAG GCAGCAGCTG GAGGAACAGA AAAATGCCCC CATGTTCCAG
    CGCATGGAGC CTTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCTTAC
    CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGGAGTG GATACCCTTC GATGAGTCCA
    CTCTCTCCCC AGGATATATT CCAGTTGGCT TGTATGGACC CCGCCGATGA CGGACAGTTC
    CAAGAACGGC AGTCTCTGGT GGTGACAGAC CCTAGCTCCA TGAGACGTTC ATTTTCCACT
    ATTCGGGATA AGCGTTCAAA TTCCTCGTGG TTGGAGGAAT TCTCCATGGA GCGAAGCAGT
    GAAAATACCT ACAAGTCCCG TCGCCGGAGT TACCACTCCT CCTTGCGGCT GTCAGCCCAC
    CGCCTGAACT CTGATTCAGG CCACAAGTCT GACACTCACC GCTCAGGGGG CAGGGAGCGG
    GGACGATCAA AAGAGCGAAA GCATCTTCTC TCTCCTGATG TCTCCCGCTG CAATTCAGAA
    GAGCGAGGGA CCCAGGCTGA CTGGGAGTCC CCAGAGCGCC GTCAATCCAG GTCACCCAGT
    GAGGGCAGGT CACAGACGCC CAACAGACAG GGCACAGGTT CCCTAAGTGA GAGCTCCATC
    CCCTCTGTCT CTGACACCAG CACCCCAAGA AGAAGTCGTC GGCAGCTCCC ACCCGTCCCG
    CCAAAGCCCC GGCCCCTCCT TTCCTACAGC TCCCTGATTC GACACGCGGG CAGCATCTCT
    CCACCTGCTG ATGGAAGCGA GGAGGGCTCC CCGCTGACCT CCCAAGCTCT GGAGAGCAAC
    AATGCTTGCC TGACCGAGTC TTCCAACTCT CCGCACCCCC AGCAGAGCCA ACATGCCTCC
    CCACAGCGCT ACATCTCCGA GCCCTACTTG GCCCTGCACG AAGACTCCCA CGCCTCAGAC
    TGTGGTGAGG AGGAGACGCT CACTTTCGAA GCAGCCGTGG CTACTAGCCT GGGCCGTTCC
    AACACCATCG GCTCAGCCCC ACCCCTGCGG CATAGCTGGC AGATGCCCAA CGGGCACTAT
    CGGCGGCGGA GGCGCGGGGG GCCTGGGCCA GGCATGATGT GTGGGGCTGT CAACAACCTG
    CTAAGTGACA CGGAAGAAGA TGACAAATGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10868
    Clone ID Related Accession (Same CDS sequence) NM_001205294.2 , NM_001205294.1
    Accession Version NM_001205294.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Homo sapiens(human)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160059.7, BP230397.1, L29384.1, AK309070.1, AB209499.1, AL161734.12, BC037800.1 and KF510705.1. On Jun 1, 2019 this sequence version replaced NM_001205294.1. Transcript Variant: This variant (2) is missing two non-consecutive in-frame coding exons compared to variant 1. This results in a shorter isoform (2, also known as alpha-1E-1) missing two internal protein segments compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L29384.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG
    CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT
    GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG
    TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC
    AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC
    GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT
    CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG
    GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG
    ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG
    AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA
    AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA
    GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA
    CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC
    AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG
    ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC
    TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC
    AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC
    AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG
    AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA
    GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT
    GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC
    ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT
    ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA
    GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCGATCGA GAGGGAGCGG AGGCGCCGGC ACCACATGTC CGTGTGGGAG
    CAGCGTACCA GCCAGCTGAG GAAGCACATG CAGATGTCCA GCCAGGAGGC CCTCAACAGA
    GAGGAGGCGC CGACCATGAA CCCGCTCAAC CCCCTCAACC CGCTCAGCTC CCTCAACCCG
    CTCAATGCCC ACCCCAGCCT TTATCGGCGA CCCAGGGCCA TTGAGGGCCT GGCCCTGGGC
    CTGGCCCTGG AGAAGTTCGA GGAGGAGCGC ATCAGCCGTG GGGGGTCCCT CAAGGGGGAT
    GGAGGGGACC GATCCAGTGC CCTGGACAAC CAGAGGACCC CTTTGTCCCT GGGCCAGCGG
    GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGAAACTGTG ACCCGACTCA GCAGGAGGCA
    GGGGGAGGAG AGGCTGTGGT GACCTTTGAG GACCGGGCCA GGCACAGGCA GAGCCAACGG
    CGCAGCCGGC ATCGCCGCGT CAGGACAGAA GGCAAGGAGT CCTCTTCAGC CTCCCGGAGC
    AGGTCTGCCA GCCAGGAACG CAGTCTGGAT GAAGCCATGC CCACTGAAGG GGAGAAGGAC
    CATGAGCTCA GGGGCAACCA TGGTGCCAAG GAGCCAACGA TCCAAGAAGA GAGAGCCCAG
    GATTTAAGGA GGACCAACAG TCTGATGGTG TCCAGAGGCT CCGGGCTGGC AGGAGGCCTT
    GATGAGGCTG ACACCCCCCT AGTCCTGCCC CATCCTGAGC TGGAAGTGGG GAAGCACGTG
    GTGCTGACGG AGCAGGAGCC AGAAGGCAGC AGTGAGCAGG CCCTGCTGGG GAATGTGCAG
    CTAGACATGG GCCGGGTCAT CAGCCAGAGC GAGCCTGACC TCTCCTGCAT CACGGCCAAC
    ACGGACAAGG CCACCACCGA GAGCACCAGC GTCACCGTCG CCATCCCCGA CGTGGACCCC
    TTGGTGGACT CAACCGTGGT GCACATTAGC AACAAGACGG ATGGGGAAGC CAGTCCCTTG
    AAGGAGGCAG AGATCAGAGA GGATGAGGAG GAGGTGGAGA AGAAGAAGCA GAAGAAGGAG
    AAGCGTGAGA CAGGCAAAGC CATGGTGCCC CACAGCTCAA TGTTCATCTT CAGCACCACC
    AACCCGATCC GGAGGGCCTG CCACTACATC GTGAACCTGC GCTACTTTGA GATGTGCATC
    CTCCTGGTGA TTGCAGCCAG CAGCATCGCC CTGGCGGCAG AGGACCCCGT CCTGACCAAC
    TCGGAGCGCA ACAAAGTCCT GAGGTATTTT GACTATGTGT TCACGGGCGT GTTCACCTTT
    GAGATGGTTA TAAAGATGAT AGACCAAGGC TTGATCCTGC AGGATGGGTC CTACTTCCGA
    GACTTGTGGA ACATCCTGGA CTTTGTGGTG GTCGTTGGCG CATTGGTGGC CTTTGCTCTG
    GCGAACGCTT TGGGAACCAA CAAAGGACGG GACATCAAGA CCATCAAGTC TCTGCGGGTG
    CTCCGAGTTC TAAGGCCACT GAAAACCATC AAGCGCTTGC CCAAGCTCAA GGCCGTCTTC
    GACTGCGTAG TGACCTCCTT GAAGAATGTC TTCAACATAC TCATTGTGTA CAAGCTCTTC
    ATGTTCATCT TTGCTGTCAT CGCAGTTCAG CTCTTCAAGG GAAAGTTCTT TTATTGCACG
    GACAGTTCCA AGGACACAGA GAAGGAGTGC ATAGGCAACT ATGTAGATCA TGAGAAAAAC
    AAGATGGAGG TGAAGGGCCG GGAATGGAAG CGCCATGAAT TCCACTACGA CAACATTATC
    TGGGCCCTGC TGACCCTCTT CACCGTCTCC ACAGGGGAAG GATGGCCTCA AGTTCTGCAG
    CACTCTGTAG ATGTGACAGA GGAAGACCGA GGCCCAAGCC GCAGCAACCG CATGGAGATG
    TCTATCTTTT ATGTAGTCTA CTTTGTGGTC TTCCCCTTCT TCTTTGTCAA TATCTTTGTG
    GCTCTCATCA TCATCACCTT CCAGGAGCAA GGGGATAAGA TGATGGAGGA GTGCAGCCTG
    GAGAAGAATG AGAGGGCGTG CATCGACTTC GCCATCAGCG CCAAACCTCT CACCCGCTAC
    ATGCCGCAGA ACAGACACAC CTTCCAGTAC CGCGTGTGGC ACTTTGTGGT GTCTCCGTCC
    TTTGAGTACA CCATTATGGC CATGATCGCC TTGAATACTG TTGTGCTGAT GATGAAGTAT
    TATTCTGCTC CCTGTACCTA TGAGCTGGCC CTGAAGTACC TGAATATCGC CTTCACCATG
    GTGTTTTCCC TGGAATGTGT CCTGAAGGTC ATCGCTTTTG GCTTTTTGAA CTATTTCCGA
    GACACCTGGA ATATCTTTGA CTTCATCACC GTGATTGGCA GTATCACAGA AATTATCCTG
    ACAGACAGCA AGCTGGTGAA CACCAGTGGC TTCAATATGA GCTTTCTGAA GCTCTTCCGA
    GCTGCCCGCC TCATAAAGCT CCTGCGTCAG GGCTATACCA TACGCATTTT GCTGTGGACC
    TTTGTGCAGT CCTTTAAGGC CCTCCCTTAT GTCTGCCTTT TAATTGCCAT GCTTTTCTTC
    ATTTATGCCA TCATTGGGAT GCAGGTATTT GGAAACATAA AATTAGACGA GGAGAGTCAC
    ATCAACCGGC ACAACAACTT CCGGAGTTTC TTTGGGTCCC TAATGCTACT CTTCAGGAGT
    GCCACAGGTG AGGCCTGGCA GGAGATTATG CTGTCATGCC TTGGGGAGAA GGGCTGTGAG
    CCTGACACCA CCGCACCATC AGGGCAGAAC GAGAACGAAC GCTGCGGCAC CGATCTGGCC
    TACGTGTACT TTGTCTCCTT CATCTTCTTC TGCTCCTTCT TGATGCTCAA CCTGTTTGTG
    GCCGTCATCA TGGACAACTT TGAGTACCTG ACTCGGGACT CCTCCATCCT GGGGCCTCAC
    CACTTGGACG AGTTTGTCCG CGTCTGGGCA GAATATGACC GAGCAGCATG TGGCCGCATC
    CATTACACTG AGATGTATGA AATGCTGACT CTCATGTCAC CTCCGCTAGG CCTCGGCAAG
    AGATGTCCCT CCAAAGTGGC ATATAAGAGG TTGGTCCTGA TGAACATGCC AGTAGCTGAG
    GACATGACGG TCCACTTCAC CTCCACACTT ATGGCTCTGA TCCGGACAGC TCTGGACATT
    AAAATTGCCA AAGGTGGTGC AGACAGGCAG CAGCTAGACT CAGAGCTACA AAAGGAGACC
    CTAGCCATCT GGCCTCACCT ATCCCAGAAG ATGCTGGATC TGCTTGTGCC CATGCCCAAA
    GCCTCTGACC TGACTGTGGG CAAAATCTAT GCAGCAATGA TGATCATGGA CTACTATAAG
    CAGAGTAAGG TGAAGAAGCA GAGGCAGCAG CTGGAGGAAC AGAAAAATGC CCCCATGTTC
    CAGCGCATGG AGCCTTCATC TCTGCCTCAG GAGATCATTG CTAATGCCAA AGCCCTGCCT
    TACCTCCAGC AGGACCCCGT TTCAGGCCTG AGTGGCCGGA GTGGATACCC TTCGATGAGT
    CCACTCTCTC CCCAGGATAT ATTCCAGTTG GCTTGTATGG ACCCCGCCGA TGACGGACAG
    TTCCAAGAAC GGCAGTCTCT GGTGGTGACA GACCCTAGCT CCATGAGACG TTCATTTTCC
    ACTATTCGGG ATAAGCGTTC AAATTCCTCG TGGTTGGAGG AATTCTCCAT GGAGCGAAGC
    AGTGAAAATA CCTACAAGTC CCGTCGCCGG AGTTACCACT CCTCCTTGCG GCTGTCAGCC
    CACCGCCTGA ACTCTGATTC AGGCCACAAG TCTGACACTC ACCGCTCAGG GGGCAGGGAG
    CGGGGACGAT CAAAAGAGCG AAAGCATCTT CTCTCTCCTG ATGTCTCCCG CTGCAATTCA
    GAAGAGCGAG GGACCCAGGC TGACTGGGAG TCCCCAGAGC GCCGTCAATC CAGGTCACCC
    AGTGAGGGCA GGTCACAGAC GCCCAACAGA CAGGGCACAG GTTCCCTAAG TGAGAGCTCC
    ATCCCCTCTG TCTCTGACAC CAGCACCCCA AGAAGAAGTC GTCGGCAGCT CCCACCCGTC
    CCGCCAAAGC CCCGGCCCCT CCTTTCCTAC AGCTCCCTGA TTCGACACGC GGGCAGCATC
    TCTCCACCTG CTGATGGAAG CGAGGAGGGC TCCCCGCTGA CCTCCCAAGC TCTGGAGAGC
    AACAATGCTT GCCTGACCGA GTCTTCCAAC TCTCCGCACC CCCAGCAGAG CCAACATGCC
    TCCCCACAGC GCTACATCTC CGAGCCCTAC TTGGCCCTGC ACGAAGACTC CCACGCCTCA
    GACTGTGGTG AGGAGGAGAC GCTCACTTTC GAAGCAGCCG TGGCTACTAG CCTGGGCCGT
    TCCAACACCA TCGGCTCAGC CCCACCCCTG CGGCATAGCT GGCAGATGCC CAACGGGCAC
    TATCGGCGGC GGAGGCGCGG GGGGCCTGGG CCAGGCATGA TGTGTGGGGC TGTCAACAAC
    CTGCTAAGTG ACACGGAAGA AGATGACAAA TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192223.1
    CDS229..6984
    Translation

    Target ORF information:

    RefSeq Version NM_001205294.2
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205294.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG
    CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT
    GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG
    TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC
    AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC
    GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT
    CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG
    GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG
    ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG
    AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA
    AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA
    GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA
    CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC
    AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG
    ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC
    TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC
    AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC
    AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG
    AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA
    GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT
    GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC
    ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT
    ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA
    GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCGATCGA GAGGGAGCGG AGGCGCCGGC ACCACATGTC CGTGTGGGAG
    CAGCGTACCA GCCAGCTGAG GAAGCACATG CAGATGTCCA GCCAGGAGGC CCTCAACAGA
    GAGGAGGCGC CGACCATGAA CCCGCTCAAC CCCCTCAACC CGCTCAGCTC CCTCAACCCG
    CTCAATGCCC ACCCCAGCCT TTATCGGCGA CCCAGGGCCA TTGAGGGCCT GGCCCTGGGC
    CTGGCCCTGG AGAAGTTCGA GGAGGAGCGC ATCAGCCGTG GGGGGTCCCT CAAGGGGGAT
    GGAGGGGACC GATCCAGTGC CCTGGACAAC CAGAGGACCC CTTTGTCCCT GGGCCAGCGG
    GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGAAACTGTG ACCCGACTCA GCAGGAGGCA
    GGGGGAGGAG AGGCTGTGGT GACCTTTGAG GACCGGGCCA GGCACAGGCA GAGCCAACGG
    CGCAGCCGGC ATCGCCGCGT CAGGACAGAA GGCAAGGAGT CCTCTTCAGC CTCCCGGAGC
    AGGTCTGCCA GCCAGGAACG CAGTCTGGAT GAAGCCATGC CCACTGAAGG GGAGAAGGAC
    CATGAGCTCA GGGGCAACCA TGGTGCCAAG GAGCCAACGA TCCAAGAAGA GAGAGCCCAG
    GATTTAAGGA GGACCAACAG TCTGATGGTG TCCAGAGGCT CCGGGCTGGC AGGAGGCCTT
    GATGAGGCTG ACACCCCCCT AGTCCTGCCC CATCCTGAGC TGGAAGTGGG GAAGCACGTG
    GTGCTGACGG AGCAGGAGCC AGAAGGCAGC AGTGAGCAGG CCCTGCTGGG GAATGTGCAG
    CTAGACATGG GCCGGGTCAT CAGCCAGAGC GAGCCTGACC TCTCCTGCAT CACGGCCAAC
    ACGGACAAGG CCACCACCGA GAGCACCAGC GTCACCGTCG CCATCCCCGA CGTGGACCCC
    TTGGTGGACT CAACCGTGGT GCACATTAGC AACAAGACGG ATGGGGAAGC CAGTCCCTTG
    AAGGAGGCAG AGATCAGAGA GGATGAGGAG GAGGTGGAGA AGAAGAAGCA GAAGAAGGAG
    AAGCGTGAGA CAGGCAAAGC CATGGTGCCC CACAGCTCAA TGTTCATCTT CAGCACCACC
    AACCCGATCC GGAGGGCCTG CCACTACATC GTGAACCTGC GCTACTTTGA GATGTGCATC
    CTCCTGGTGA TTGCAGCCAG CAGCATCGCC CTGGCGGCAG AGGACCCCGT CCTGACCAAC
    TCGGAGCGCA ACAAAGTCCT GAGGTATTTT GACTATGTGT TCACGGGCGT GTTCACCTTT
    GAGATGGTTA TAAAGATGAT AGACCAAGGC TTGATCCTGC AGGATGGGTC CTACTTCCGA
    GACTTGTGGA ACATCCTGGA CTTTGTGGTG GTCGTTGGCG CATTGGTGGC CTTTGCTCTG
    GCGAACGCTT TGGGAACCAA CAAAGGACGG GACATCAAGA CCATCAAGTC TCTGCGGGTG
    CTCCGAGTTC TAAGGCCACT GAAAACCATC AAGCGCTTGC CCAAGCTCAA GGCCGTCTTC
    GACTGCGTAG TGACCTCCTT GAAGAATGTC TTCAACATAC TCATTGTGTA CAAGCTCTTC
    ATGTTCATCT TTGCTGTCAT CGCAGTTCAG CTCTTCAAGG GAAAGTTCTT TTATTGCACG
    GACAGTTCCA AGGACACAGA GAAGGAGTGC ATAGGCAACT ATGTAGATCA TGAGAAAAAC
    AAGATGGAGG TGAAGGGCCG GGAATGGAAG CGCCATGAAT TCCACTACGA CAACATTATC
    TGGGCCCTGC TGACCCTCTT CACCGTCTCC ACAGGGGAAG GATGGCCTCA AGTTCTGCAG
    CACTCTGTAG ATGTGACAGA GGAAGACCGA GGCCCAAGCC GCAGCAACCG CATGGAGATG
    TCTATCTTTT ATGTAGTCTA CTTTGTGGTC TTCCCCTTCT TCTTTGTCAA TATCTTTGTG
    GCTCTCATCA TCATCACCTT CCAGGAGCAA GGGGATAAGA TGATGGAGGA GTGCAGCCTG
    GAGAAGAATG AGAGGGCGTG CATCGACTTC GCCATCAGCG CCAAACCTCT CACCCGCTAC
    ATGCCGCAGA ACAGACACAC CTTCCAGTAC CGCGTGTGGC ACTTTGTGGT GTCTCCGTCC
    TTTGAGTACA CCATTATGGC CATGATCGCC TTGAATACTG TTGTGCTGAT GATGAAGTAT
    TATTCTGCTC CCTGTACCTA TGAGCTGGCC CTGAAGTACC TGAATATCGC CTTCACCATG
    GTGTTTTCCC TGGAATGTGT CCTGAAGGTC ATCGCTTTTG GCTTTTTGAA CTATTTCCGA
    GACACCTGGA ATATCTTTGA CTTCATCACC GTGATTGGCA GTATCACAGA AATTATCCTG
    ACAGACAGCA AGCTGGTGAA CACCAGTGGC TTCAATATGA GCTTTCTGAA GCTCTTCCGA
    GCTGCCCGCC TCATAAAGCT CCTGCGTCAG GGCTATACCA TACGCATTTT GCTGTGGACC
    TTTGTGCAGT CCTTTAAGGC CCTCCCTTAT GTCTGCCTTT TAATTGCCAT GCTTTTCTTC
    ATTTATGCCA TCATTGGGAT GCAGGTATTT GGAAACATAA AATTAGACGA GGAGAGTCAC
    ATCAACCGGC ACAACAACTT CCGGAGTTTC TTTGGGTCCC TAATGCTACT CTTCAGGAGT
    GCCACAGGTG AGGCCTGGCA GGAGATTATG CTGTCATGCC TTGGGGAGAA GGGCTGTGAG
    CCTGACACCA CCGCACCATC AGGGCAGAAC GAGAACGAAC GCTGCGGCAC CGATCTGGCC
    TACGTGTACT TTGTCTCCTT CATCTTCTTC TGCTCCTTCT TGATGCTCAA CCTGTTTGTG
    GCCGTCATCA TGGACAACTT TGAGTACCTG ACTCGGGACT CCTCCATCCT GGGGCCTCAC
    CACTTGGACG AGTTTGTCCG CGTCTGGGCA GAATATGACC GAGCAGCATG TGGCCGCATC
    CATTACACTG AGATGTATGA AATGCTGACT CTCATGTCAC CTCCGCTAGG CCTCGGCAAG
    AGATGTCCCT CCAAAGTGGC ATATAAGAGG TTGGTCCTGA TGAACATGCC AGTAGCTGAG
    GACATGACGG TCCACTTCAC CTCCACACTT ATGGCTCTGA TCCGGACAGC TCTGGACATT
    AAAATTGCCA AAGGTGGTGC AGACAGGCAG CAGCTAGACT CAGAGCTACA AAAGGAGACC
    CTAGCCATCT GGCCTCACCT ATCCCAGAAG ATGCTGGATC TGCTTGTGCC CATGCCCAAA
    GCCTCTGACC TGACTGTGGG CAAAATCTAT GCAGCAATGA TGATCATGGA CTACTATAAG
    CAGAGTAAGG TGAAGAAGCA GAGGCAGCAG CTGGAGGAAC AGAAAAATGC CCCCATGTTC
    CAGCGCATGG AGCCTTCATC TCTGCCTCAG GAGATCATTG CTAATGCCAA AGCCCTGCCT
    TACCTCCAGC AGGACCCCGT TTCAGGCCTG AGTGGCCGGA GTGGATACCC TTCGATGAGT
    CCACTCTCTC CCCAGGATAT ATTCCAGTTG GCTTGTATGG ACCCCGCCGA TGACGGACAG
    TTCCAAGAAC GGCAGTCTCT GGTGGTGACA GACCCTAGCT CCATGAGACG TTCATTTTCC
    ACTATTCGGG ATAAGCGTTC AAATTCCTCG TGGTTGGAGG AATTCTCCAT GGAGCGAAGC
    AGTGAAAATA CCTACAAGTC CCGTCGCCGG AGTTACCACT CCTCCTTGCG GCTGTCAGCC
    CACCGCCTGA ACTCTGATTC AGGCCACAAG TCTGACACTC ACCGCTCAGG GGGCAGGGAG
    CGGGGACGAT CAAAAGAGCG AAAGCATCTT CTCTCTCCTG ATGTCTCCCG CTGCAATTCA
    GAAGAGCGAG GGACCCAGGC TGACTGGGAG TCCCCAGAGC GCCGTCAATC CAGGTCACCC
    AGTGAGGGCA GGTCACAGAC GCCCAACAGA CAGGGCACAG GTTCCCTAAG TGAGAGCTCC
    ATCCCCTCTG TCTCTGACAC CAGCACCCCA AGAAGAAGTC GTCGGCAGCT CCCACCCGTC
    CCGCCAAAGC CCCGGCCCCT CCTTTCCTAC AGCTCCCTGA TTCGACACGC GGGCAGCATC
    TCTCCACCTG CTGATGGAAG CGAGGAGGGC TCCCCGCTGA CCTCCCAAGC TCTGGAGAGC
    AACAATGCTT GCCTGACCGA GTCTTCCAAC TCTCCGCACC CCCAGCAGAG CCAACATGCC
    TCCCCACAGC GCTACATCTC CGAGCCCTAC TTGGCCCTGC ACGAAGACTC CCACGCCTCA
    GACTGTGGTG AGGAGGAGAC GCTCACTTTC GAAGCAGCCG TGGCTACTAG CCTGGGCCGT
    TCCAACACCA TCGGCTCAGC CCCACCCCTG CGGCATAGCT GGCAGATGCC CAACGGGCAC
    TATCGGCGGC GGAGGCGCGG GGGGCCTGGG CCAGGCATGA TGTGTGGGGC TGTCAACAAC
    CTGCTAAGTG ACACGGAAGA AGATGACAAA TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10868
    Clone ID Related Accession (Same CDS sequence) NM_001205294.2 , NM_001205294.1
    Accession Version NM_001205294.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-31
    Organism Homo sapiens(human)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP230397.1, L29384.1, AK309070.1, AB209499.1, AL161734.12 and BC037800.1. Transcript Variant: This variant (2) is missing two non-consecutive in-frame coding exons compared to variant 1. This results in a shorter isoform (2, also known as alpha-1E-1) missing two internal protein segments compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L29384.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG
    CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT
    GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG
    TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC
    AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC
    GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT
    CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG
    GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG
    ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG
    AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA
    AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA
    GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA
    CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC
    AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG
    ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC
    TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC
    AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC
    AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG
    AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA
    GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT
    GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC
    ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT
    ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA
    GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCGATCGA GAGGGAGCGG AGGCGCCGGC ACCACATGTC CGTGTGGGAG
    CAGCGTACCA GCCAGCTGAG GAAGCACATG CAGATGTCCA GCCAGGAGGC CCTCAACAGA
    GAGGAGGCGC CGACCATGAA CCCGCTCAAC CCCCTCAACC CGCTCAGCTC CCTCAACCCG
    CTCAATGCCC ACCCCAGCCT TTATCGGCGA CCCAGGGCCA TTGAGGGCCT GGCCCTGGGC
    CTGGCCCTGG AGAAGTTCGA GGAGGAGCGC ATCAGCCGTG GGGGGTCCCT CAAGGGGGAT
    GGAGGGGACC GATCCAGTGC CCTGGACAAC CAGAGGACCC CTTTGTCCCT GGGCCAGCGG
    GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGAAACTGTG ACCCGACTCA GCAGGAGGCA
    GGGGGAGGAG AGGCTGTGGT GACCTTTGAG GACCGGGCCA GGCACAGGCA GAGCCAACGG
    CGCAGCCGGC ATCGCCGCGT CAGGACAGAA GGCAAGGAGT CCTCTTCAGC CTCCCGGAGC
    AGGTCTGCCA GCCAGGAACG CAGTCTGGAT GAAGCCATGC CCACTGAAGG GGAGAAGGAC
    CATGAGCTCA GGGGCAACCA TGGTGCCAAG GAGCCAACGA TCCAAGAAGA GAGAGCCCAG
    GATTTAAGGA GGACCAACAG TCTGATGGTG TCCAGAGGCT CCGGGCTGGC AGGAGGCCTT
    GATGAGGCTG ACACCCCCCT AGTCCTGCCC CATCCTGAGC TGGAAGTGGG GAAGCACGTG
    GTGCTGACGG AGCAGGAGCC AGAAGGCAGC AGTGAGCAGG CCCTGCTGGG GAATGTGCAG
    CTAGACATGG GCCGGGTCAT CAGCCAGAGC GAGCCTGACC TCTCCTGCAT CACGGCCAAC
    ACGGACAAGG CCACCACCGA GAGCACCAGC GTCACCGTCG CCATCCCCGA CGTGGACCCC
    TTGGTGGACT CAACCGTGGT GCACATTAGC AACAAGACGG ATGGGGAAGC CAGTCCCTTG
    AAGGAGGCAG AGATCAGAGA GGATGAGGAG GAGGTGGAGA AGAAGAAGCA GAAGAAGGAG
    AAGCGTGAGA CAGGCAAAGC CATGGTGCCC CACAGCTCAA TGTTCATCTT CAGCACCACC
    AACCCGATCC GGAGGGCCTG CCACTACATC GTGAACCTGC GCTACTTTGA GATGTGCATC
    CTCCTGGTGA TTGCAGCCAG CAGCATCGCC CTGGCGGCAG AGGACCCCGT CCTGACCAAC
    TCGGAGCGCA ACAAAGTCCT GAGGTATTTT GACTATGTGT TCACGGGCGT GTTCACCTTT
    GAGATGGTTA TAAAGATGAT AGACCAAGGC TTGATCCTGC AGGATGGGTC CTACTTCCGA
    GACTTGTGGA ACATCCTGGA CTTTGTGGTG GTCGTTGGCG CATTGGTGGC CTTTGCTCTG
    GCGAACGCTT TGGGAACCAA CAAAGGACGG GACATCAAGA CCATCAAGTC TCTGCGGGTG
    CTCCGAGTTC TAAGGCCACT GAAAACCATC AAGCGCTTGC CCAAGCTCAA GGCCGTCTTC
    GACTGCGTAG TGACCTCCTT GAAGAATGTC TTCAACATAC TCATTGTGTA CAAGCTCTTC
    ATGTTCATCT TTGCTGTCAT CGCAGTTCAG CTCTTCAAGG GAAAGTTCTT TTATTGCACG
    GACAGTTCCA AGGACACAGA GAAGGAGTGC ATAGGCAACT ATGTAGATCA TGAGAAAAAC
    AAGATGGAGG TGAAGGGCCG GGAATGGAAG CGCCATGAAT TCCACTACGA CAACATTATC
    TGGGCCCTGC TGACCCTCTT CACCGTCTCC ACAGGGGAAG GATGGCCTCA AGTTCTGCAG
    CACTCTGTAG ATGTGACAGA GGAAGACCGA GGCCCAAGCC GCAGCAACCG CATGGAGATG
    TCTATCTTTT ATGTAGTCTA CTTTGTGGTC TTCCCCTTCT TCTTTGTCAA TATCTTTGTG
    GCTCTCATCA TCATCACCTT CCAGGAGCAA GGGGATAAGA TGATGGAGGA GTGCAGCCTG
    GAGAAGAATG AGAGGGCGTG CATCGACTTC GCCATCAGCG CCAAACCTCT CACCCGCTAC
    ATGCCGCAGA ACAGACACAC CTTCCAGTAC CGCGTGTGGC ACTTTGTGGT GTCTCCGTCC
    TTTGAGTACA CCATTATGGC CATGATCGCC TTGAATACTG TTGTGCTGAT GATGAAGTAT
    TATTCTGCTC CCTGTACCTA TGAGCTGGCC CTGAAGTACC TGAATATCGC CTTCACCATG
    GTGTTTTCCC TGGAATGTGT CCTGAAGGTC ATCGCTTTTG GCTTTTTGAA CTATTTCCGA
    GACACCTGGA ATATCTTTGA CTTCATCACC GTGATTGGCA GTATCACAGA AATTATCCTG
    ACAGACAGCA AGCTGGTGAA CACCAGTGGC TTCAATATGA GCTTTCTGAA GCTCTTCCGA
    GCTGCCCGCC TCATAAAGCT CCTGCGTCAG GGCTATACCA TACGCATTTT GCTGTGGACC
    TTTGTGCAGT CCTTTAAGGC CCTCCCTTAT GTCTGCCTTT TAATTGCCAT GCTTTTCTTC
    ATTTATGCCA TCATTGGGAT GCAGGTATTT GGAAACATAA AATTAGACGA GGAGAGTCAC
    ATCAACCGGC ACAACAACTT CCGGAGTTTC TTTGGGTCCC TAATGCTACT CTTCAGGAGT
    GCCACAGGTG AGGCCTGGCA GGAGATTATG CTGTCATGCC TTGGGGAGAA GGGCTGTGAG
    CCTGACACCA CCGCACCATC AGGGCAGAAC GAGAACGAAC GCTGCGGCAC CGATCTGGCC
    TACGTGTACT TTGTCTCCTT CATCTTCTTC TGCTCCTTCT TGATGCTCAA CCTGTTTGTG
    GCCGTCATCA TGGACAACTT TGAGTACCTG ACTCGGGACT CCTCCATCCT GGGGCCTCAC
    CACTTGGACG AGTTTGTCCG CGTCTGGGCA GAATATGACC GAGCAGCATG TGGCCGCATC
    CATTACACTG AGATGTATGA AATGCTGACT CTCATGTCAC CTCCGCTAGG CCTCGGCAAG
    AGATGTCCCT CCAAAGTGGC ATATAAGAGG TTGGTCCTGA TGAACATGCC AGTAGCTGAG
    GACATGACGG TCCACTTCAC CTCCACACTT ATGGCTCTGA TCCGGACAGC TCTGGACATT
    AAAATTGCCA AAGGTGGTGC AGACAGGCAG CAGCTAGACT CAGAGCTACA AAAGGAGACC
    CTAGCCATCT GGCCTCACCT ATCCCAGAAG ATGCTGGATC TGCTTGTGCC CATGCCCAAA
    GCCTCTGACC TGACTGTGGG CAAAATCTAT GCAGCAATGA TGATCATGGA CTACTATAAG
    CAGAGTAAGG TGAAGAAGCA GAGGCAGCAG CTGGAGGAAC AGAAAAATGC CCCCATGTTC
    CAGCGCATGG AGCCTTCATC TCTGCCTCAG GAGATCATTG CTAATGCCAA AGCCCTGCCT
    TACCTCCAGC AGGACCCCGT TTCAGGCCTG AGTGGCCGGA GTGGATACCC TTCGATGAGT
    CCACTCTCTC CCCAGGATAT ATTCCAGTTG GCTTGTATGG ACCCCGCCGA TGACGGACAG
    TTCCAAGAAC GGCAGTCTCT GGTGGTGACA GACCCTAGCT CCATGAGACG TTCATTTTCC
    ACTATTCGGG ATAAGCGTTC AAATTCCTCG TGGTTGGAGG AATTCTCCAT GGAGCGAAGC
    AGTGAAAATA CCTACAAGTC CCGTCGCCGG AGTTACCACT CCTCCTTGCG GCTGTCAGCC
    CACCGCCTGA ACTCTGATTC AGGCCACAAG TCTGACACTC ACCGCTCAGG GGGCAGGGAG
    CGGGGACGAT CAAAAGAGCG AAAGCATCTT CTCTCTCCTG ATGTCTCCCG CTGCAATTCA
    GAAGAGCGAG GGACCCAGGC TGACTGGGAG TCCCCAGAGC GCCGTCAATC CAGGTCACCC
    AGTGAGGGCA GGTCACAGAC GCCCAACAGA CAGGGCACAG GTTCCCTAAG TGAGAGCTCC
    ATCCCCTCTG TCTCTGACAC CAGCACCCCA AGAAGAAGTC GTCGGCAGCT CCCACCCGTC
    CCGCCAAAGC CCCGGCCCCT CCTTTCCTAC AGCTCCCTGA TTCGACACGC GGGCAGCATC
    TCTCCACCTG CTGATGGAAG CGAGGAGGGC TCCCCGCTGA CCTCCCAAGC TCTGGAGAGC
    AACAATGCTT GCCTGACCGA GTCTTCCAAC TCTCCGCACC CCCAGCAGAG CCAACATGCC
    TCCCCACAGC GCTACATCTC CGAGCCCTAC TTGGCCCTGC ACGAAGACTC CCACGCCTCA
    GACTGTGGTG AGGAGGAGAC GCTCACTTTC GAAGCAGCCG TGGCTACTAG CCTGGGCCGT
    TCCAACACCA TCGGCTCAGC CCCACCCCTG CGGCATAGCT GGCAGATGCC CAACGGGCAC
    TATCGGCGGC GGAGGCGCGG GGGGCCTGGG CCAGGCATGA TGTGTGGGGC TGTCAACAAC
    CTGCTAAGTG ACACGGAAGA AGATGACAAA TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192223.1
    CDS196..6951
    Translation

    Target ORF information:

    RefSeq Version NM_001205294.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205294.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG
    CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT
    GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG
    TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC
    AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC
    GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT
    CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG
    GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG
    ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG
    AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA
    AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA
    GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA
    CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC
    AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG
    ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC
    TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC
    AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC
    AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG
    AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA
    GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT
    GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC
    ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT
    ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA
    GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCGATCGA GAGGGAGCGG AGGCGCCGGC ACCACATGTC CGTGTGGGAG
    CAGCGTACCA GCCAGCTGAG GAAGCACATG CAGATGTCCA GCCAGGAGGC CCTCAACAGA
    GAGGAGGCGC CGACCATGAA CCCGCTCAAC CCCCTCAACC CGCTCAGCTC CCTCAACCCG
    CTCAATGCCC ACCCCAGCCT TTATCGGCGA CCCAGGGCCA TTGAGGGCCT GGCCCTGGGC
    CTGGCCCTGG AGAAGTTCGA GGAGGAGCGC ATCAGCCGTG GGGGGTCCCT CAAGGGGGAT
    GGAGGGGACC GATCCAGTGC CCTGGACAAC CAGAGGACCC CTTTGTCCCT GGGCCAGCGG
    GAGCCACCAT GGCTGGCCAG GCCCTGTCAT GGAAACTGTG ACCCGACTCA GCAGGAGGCA
    GGGGGAGGAG AGGCTGTGGT GACCTTTGAG GACCGGGCCA GGCACAGGCA GAGCCAACGG
    CGCAGCCGGC ATCGCCGCGT CAGGACAGAA GGCAAGGAGT CCTCTTCAGC CTCCCGGAGC
    AGGTCTGCCA GCCAGGAACG CAGTCTGGAT GAAGCCATGC CCACTGAAGG GGAGAAGGAC
    CATGAGCTCA GGGGCAACCA TGGTGCCAAG GAGCCAACGA TCCAAGAAGA GAGAGCCCAG
    GATTTAAGGA GGACCAACAG TCTGATGGTG TCCAGAGGCT CCGGGCTGGC AGGAGGCCTT
    GATGAGGCTG ACACCCCCCT AGTCCTGCCC CATCCTGAGC TGGAAGTGGG GAAGCACGTG
    GTGCTGACGG AGCAGGAGCC AGAAGGCAGC AGTGAGCAGG CCCTGCTGGG GAATGTGCAG
    CTAGACATGG GCCGGGTCAT CAGCCAGAGC GAGCCTGACC TCTCCTGCAT CACGGCCAAC
    ACGGACAAGG CCACCACCGA GAGCACCAGC GTCACCGTCG CCATCCCCGA CGTGGACCCC
    TTGGTGGACT CAACCGTGGT GCACATTAGC AACAAGACGG ATGGGGAAGC CAGTCCCTTG
    AAGGAGGCAG AGATCAGAGA GGATGAGGAG GAGGTGGAGA AGAAGAAGCA GAAGAAGGAG
    AAGCGTGAGA CAGGCAAAGC CATGGTGCCC CACAGCTCAA TGTTCATCTT CAGCACCACC
    AACCCGATCC GGAGGGCCTG CCACTACATC GTGAACCTGC GCTACTTTGA GATGTGCATC
    CTCCTGGTGA TTGCAGCCAG CAGCATCGCC CTGGCGGCAG AGGACCCCGT CCTGACCAAC
    TCGGAGCGCA ACAAAGTCCT GAGGTATTTT GACTATGTGT TCACGGGCGT GTTCACCTTT
    GAGATGGTTA TAAAGATGAT AGACCAAGGC TTGATCCTGC AGGATGGGTC CTACTTCCGA
    GACTTGTGGA ACATCCTGGA CTTTGTGGTG GTCGTTGGCG CATTGGTGGC CTTTGCTCTG
    GCGAACGCTT TGGGAACCAA CAAAGGACGG GACATCAAGA CCATCAAGTC TCTGCGGGTG
    CTCCGAGTTC TAAGGCCACT GAAAACCATC AAGCGCTTGC CCAAGCTCAA GGCCGTCTTC
    GACTGCGTAG TGACCTCCTT GAAGAATGTC TTCAACATAC TCATTGTGTA CAAGCTCTTC
    ATGTTCATCT TTGCTGTCAT CGCAGTTCAG CTCTTCAAGG GAAAGTTCTT TTATTGCACG
    GACAGTTCCA AGGACACAGA GAAGGAGTGC ATAGGCAACT ATGTAGATCA TGAGAAAAAC
    AAGATGGAGG TGAAGGGCCG GGAATGGAAG CGCCATGAAT TCCACTACGA CAACATTATC
    TGGGCCCTGC TGACCCTCTT CACCGTCTCC ACAGGGGAAG GATGGCCTCA AGTTCTGCAG
    CACTCTGTAG ATGTGACAGA GGAAGACCGA GGCCCAAGCC GCAGCAACCG CATGGAGATG
    TCTATCTTTT ATGTAGTCTA CTTTGTGGTC TTCCCCTTCT TCTTTGTCAA TATCTTTGTG
    GCTCTCATCA TCATCACCTT CCAGGAGCAA GGGGATAAGA TGATGGAGGA GTGCAGCCTG
    GAGAAGAATG AGAGGGCGTG CATCGACTTC GCCATCAGCG CCAAACCTCT CACCCGCTAC
    ATGCCGCAGA ACAGACACAC CTTCCAGTAC CGCGTGTGGC ACTTTGTGGT GTCTCCGTCC
    TTTGAGTACA CCATTATGGC CATGATCGCC TTGAATACTG TTGTGCTGAT GATGAAGTAT
    TATTCTGCTC CCTGTACCTA TGAGCTGGCC CTGAAGTACC TGAATATCGC CTTCACCATG
    GTGTTTTCCC TGGAATGTGT CCTGAAGGTC ATCGCTTTTG GCTTTTTGAA CTATTTCCGA
    GACACCTGGA ATATCTTTGA CTTCATCACC GTGATTGGCA GTATCACAGA AATTATCCTG
    ACAGACAGCA AGCTGGTGAA CACCAGTGGC TTCAATATGA GCTTTCTGAA GCTCTTCCGA
    GCTGCCCGCC TCATAAAGCT CCTGCGTCAG GGCTATACCA TACGCATTTT GCTGTGGACC
    TTTGTGCAGT CCTTTAAGGC CCTCCCTTAT GTCTGCCTTT TAATTGCCAT GCTTTTCTTC
    ATTTATGCCA TCATTGGGAT GCAGGTATTT GGAAACATAA AATTAGACGA GGAGAGTCAC
    ATCAACCGGC ACAACAACTT CCGGAGTTTC TTTGGGTCCC TAATGCTACT CTTCAGGAGT
    GCCACAGGTG AGGCCTGGCA GGAGATTATG CTGTCATGCC TTGGGGAGAA GGGCTGTGAG
    CCTGACACCA CCGCACCATC AGGGCAGAAC GAGAACGAAC GCTGCGGCAC CGATCTGGCC
    TACGTGTACT TTGTCTCCTT CATCTTCTTC TGCTCCTTCT TGATGCTCAA CCTGTTTGTG
    GCCGTCATCA TGGACAACTT TGAGTACCTG ACTCGGGACT CCTCCATCCT GGGGCCTCAC
    CACTTGGACG AGTTTGTCCG CGTCTGGGCA GAATATGACC GAGCAGCATG TGGCCGCATC
    CATTACACTG AGATGTATGA AATGCTGACT CTCATGTCAC CTCCGCTAGG CCTCGGCAAG
    AGATGTCCCT CCAAAGTGGC ATATAAGAGG TTGGTCCTGA TGAACATGCC AGTAGCTGAG
    GACATGACGG TCCACTTCAC CTCCACACTT ATGGCTCTGA TCCGGACAGC TCTGGACATT
    AAAATTGCCA AAGGTGGTGC AGACAGGCAG CAGCTAGACT CAGAGCTACA AAAGGAGACC
    CTAGCCATCT GGCCTCACCT ATCCCAGAAG ATGCTGGATC TGCTTGTGCC CATGCCCAAA
    GCCTCTGACC TGACTGTGGG CAAAATCTAT GCAGCAATGA TGATCATGGA CTACTATAAG
    CAGAGTAAGG TGAAGAAGCA GAGGCAGCAG CTGGAGGAAC AGAAAAATGC CCCCATGTTC
    CAGCGCATGG AGCCTTCATC TCTGCCTCAG GAGATCATTG CTAATGCCAA AGCCCTGCCT
    TACCTCCAGC AGGACCCCGT TTCAGGCCTG AGTGGCCGGA GTGGATACCC TTCGATGAGT
    CCACTCTCTC CCCAGGATAT ATTCCAGTTG GCTTGTATGG ACCCCGCCGA TGACGGACAG
    TTCCAAGAAC GGCAGTCTCT GGTGGTGACA GACCCTAGCT CCATGAGACG TTCATTTTCC
    ACTATTCGGG ATAAGCGTTC AAATTCCTCG TGGTTGGAGG AATTCTCCAT GGAGCGAAGC
    AGTGAAAATA CCTACAAGTC CCGTCGCCGG AGTTACCACT CCTCCTTGCG GCTGTCAGCC
    CACCGCCTGA ACTCTGATTC AGGCCACAAG TCTGACACTC ACCGCTCAGG GGGCAGGGAG
    CGGGGACGAT CAAAAGAGCG AAAGCATCTT CTCTCTCCTG ATGTCTCCCG CTGCAATTCA
    GAAGAGCGAG GGACCCAGGC TGACTGGGAG TCCCCAGAGC GCCGTCAATC CAGGTCACCC
    AGTGAGGGCA GGTCACAGAC GCCCAACAGA CAGGGCACAG GTTCCCTAAG TGAGAGCTCC
    ATCCCCTCTG TCTCTGACAC CAGCACCCCA AGAAGAAGTC GTCGGCAGCT CCCACCCGTC
    CCGCCAAAGC CCCGGCCCCT CCTTTCCTAC AGCTCCCTGA TTCGACACGC GGGCAGCATC
    TCTCCACCTG CTGATGGAAG CGAGGAGGGC TCCCCGCTGA CCTCCCAAGC TCTGGAGAGC
    AACAATGCTT GCCTGACCGA GTCTTCCAAC TCTCCGCACC CCCAGCAGAG CCAACATGCC
    TCCCCACAGC GCTACATCTC CGAGCCCTAC TTGGCCCTGC ACGAAGACTC CCACGCCTCA
    GACTGTGGTG AGGAGGAGAC GCTCACTTTC GAAGCAGCCG TGGCTACTAG CCTGGGCCGT
    TCCAACACCA TCGGCTCAGC CCCACCCCTG CGGCATAGCT GGCAGATGCC CAACGGGCAC
    TATCGGCGGC GGAGGCGCGG GGGGCCTGGG CCAGGCATGA TGTGTGGGGC TGTCAACAAC
    CTGCTAAGTG ACACGGAAGA AGATGACAAA TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11422
    Clone ID Related Accession (Same CDS sequence) NM_001205293.1 , NM_001205293.3
    Accession Version NM_001205293.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-11-01
    Organism Homo sapiens(human)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP230397.1, L27745.1, L29385.1, AB209499.1, AL161734.12 and BC037800.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript, and encodes the longest isoform (1, also known as alpha-1E). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L27745.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG
    CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT
    GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG
    TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC
    AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC
    GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT
    CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG
    GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG
    ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG
    AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA
    AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA
    GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA
    CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC
    AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG
    ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC
    TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC
    AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC
    AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG
    AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA
    GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT
    GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC
    ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT
    ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA
    GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCGATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCCGT GTGGGAGCAG
    CGTACCAGCC AGCTGAGGAA GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAGAGAG
    GAGGCGCCGA CCATGAACCC GCTCAACCCC CTCAACCCGC TCAGCTCCCT CAACCCGCTC
    AATGCCCACC CCAGCCTTTA TCGGCGACCC AGGGCCATTG AGGGCCTGGC CCTGGGCCTG
    GCCCTGGAGA AGTTCGAGGA GGAGCGCATC AGCCGTGGGG GGTCCCTCAA GGGGGATGGA
    GGGGACCGAT CCAGTGCCCT GGACAACCAG AGGACCCCTT TGTCCCTGGG CCAGCGGGAG
    CCACCATGGC TGGCCAGGCC CTGTCATGGA AACTGTGACC CGACTCAGCA GGAGGCAGGG
    GGAGGAGAGG CTGTGGTGAC CTTTGAGGAC CGGGCCAGGC ACAGGCAGAG CCAACGGCGC
    AGCCGGCATC GCCGCGTCAG GACAGAAGGC AAGGAGTCCT CTTCAGCCTC CCGGAGCAGG
    TCTGCCAGCC AGGAACGCAG TCTGGATGAA GCCATGCCCA CTGAAGGGGA GAAGGACCAT
    GAGCTCAGGG GCAACCATGG TGCCAAGGAG CCAACGATCC AAGAAGAGAG AGCCCAGGAT
    TTAAGGAGGA CCAACAGTCT GATGGTGTCC AGAGGCTCCG GGCTGGCAGG AGGCCTTGAT
    GAGGCTGACA CCCCCCTAGT CCTGCCCCAT CCTGAGCTGG AAGTGGGGAA GCACGTGGTG
    CTGACGGAGC AGGAGCCAGA AGGCAGCAGT GAGCAGGCCC TGCTGGGGAA TGTGCAGCTA
    GACATGGGCC GGGTCATCAG CCAGAGCGAG CCTGACCTCT CCTGCATCAC GGCCAACACG
    GACAAGGCCA CCACCGAGAG CACCAGCGTC ACCGTCGCCA TCCCCGACGT GGACCCCTTG
    GTGGACTCAA CCGTGGTGCA CATTAGCAAC AAGACGGATG GGGAAGCCAG TCCCTTGAAG
    GAGGCAGAGA TCAGAGAGGA TGAGGAGGAG GTGGAGAAGA AGAAGCAGAA GAAGGAGAAG
    CGTGAGACAG GCAAAGCCAT GGTGCCCCAC AGCTCAATGT TCATCTTCAG CACCACCAAC
    CCGATCCGGA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATCCTC
    CTGGTGATTG CAGCCAGCAG CATCGCCCTG GCGGCAGAGG ACCCCGTCCT GACCAACTCG
    GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTGTTCA CGGGCGTGTT CACCTTTGAG
    ATGGTTATAA AGATGATAGA CCAAGGCTTG ATCCTGCAGG ATGGGTCCTA CTTCCGAGAC
    TTGTGGAACA TCCTGGACTT TGTGGTGGTC GTTGGCGCAT TGGTGGCCTT TGCTCTGGCG
    AACGCTTTGG GAACCAACAA AGGACGGGAC ATCAAGACCA TCAAGTCTCT GCGGGTGCTC
    CGAGTTCTAA GGCCACTGAA AACCATCAAG CGCTTGCCCA AGCTCAAGGC CGTCTTCGAC
    TGCGTAGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TTGTGTACAA GCTCTTCATG
    TTCATCTTTG CTGTCATCGC AGTTCAGCTC TTCAAGGGAA AGTTCTTTTA TTGCACGGAC
    AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTATG TAGATCATGA GAAAAACAAG
    ATGGAGGTGA AGGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG
    GCCCTGCTGA CCCTCTTCAC CGTCTCCACA GGGGAAGGAT GGCCTCAAGT TCTGCAGCAC
    TCTGTAGATG TGACAGAGGA AGACCGAGGC CCAAGCCGCA GCAACCGCAT GGAGATGTCT
    ATCTTTTATG TAGTCTACTT TGTGGTCTTC CCCTTCTTCT TTGTCAATAT CTTTGTGGCT
    CTCATCATCA TCACCTTCCA GGAGCAAGGG GATAAGATGA TGGAGGAGTG CAGCCTGGAG
    AAGAATGAGA GGGCGTGCAT CGACTTCGCC ATCAGCGCCA AACCTCTCAC CCGCTACATG
    CCGCAGAACA GACACACCTT CCAGTACCGC GTGTGGCACT TTGTGGTGTC TCCGTCCTTT
    GAGTACACCA TTATGGCCAT GATCGCCTTG AATACTGTTG TGCTGATGAT GAAGTATTAT
    TCTGCTCCCT GTACCTATGA GCTGGCCCTG AAGTACCTGA ATATCGCCTT CACCATGGTG
    TTTTCCCTGG AATGTGTCCT GAAGGTCATC GCTTTTGGCT TTTTGAACTA TTTCCGAGAC
    ACCTGGAATA TCTTTGACTT CATCACCGTG ATTGGCAGTA TCACAGAAAT TATCCTGACA
    GACAGCAAGC TGGTGAACAC CAGTGGCTTC AATATGAGCT TTCTGAAGCT CTTCCGAGCT
    GCCCGCCTCA TAAAGCTCCT GCGTCAGGGC TATACCATAC GCATTTTGCT GTGGACCTTT
    GTGCAGTCCT TTAAGGCCCT CCCTTATGTC TGCCTTTTAA TTGCCATGCT TTTCTTCATT
    TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGACGAGGA GAGTCACATC
    AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTAA TGCTACTCTT CAGGAGTGCC
    ACAGGTGAGG CCTGGCAGGA GATTATGCTG TCATGCCTTG GGGAGAAGGG CTGTGAGCCT
    GACACCACCG CACCATCAGG GCAGAACGAG AACGAACGCT GCGGCACCGA TCTGGCCTAC
    GTGTACTTTG TCTCCTTCAT CTTCTTCTGC TCCTTCTTGA TGCTCAACCT GTTTGTGGCC
    GTCATCATGG ACAACTTTGA GTACCTGACT CGGGACTCCT CCATCCTGGG GCCTCACCAC
    TTGGACGAGT TTGTCCGCGT CTGGGCAGAA TATGACCGAG CAGCATGTGG CCGCATCCAT
    TACACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCTC CGCTAGGCCT CGGCAAGAGA
    TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTGATGA ACATGCCAGT AGCTGAGGAC
    ATGACGGTCC ACTTCACCTC CACACTTATG GCTCTGATCC GGACAGCTCT GGACATTAAA
    ATTGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCAG AGCTACAAAA GGAGACCCTA
    GCCATCTGGC CTCACCTATC CCAGAAGATG CTGGATCTGC TTGTGCCCAT GCCCAAAGCC
    TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAGCAG
    AGTAAGGTGA AGAAGCAGAG GCAGCAGCTG GAGGAACAGA AAAATGCCCC CATGTTCCAG
    CGCATGGAGC CTTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCTTAC
    CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGGAGTG GATACCCTTC GATGAGTCCA
    CTCTCTCCCC AGGATATATT CCAGTTGGCT TGTATGGACC CCGCCGATGA CGGACAGTTC
    CAAGAACGGC AGTCTCTGGA GCCAGAGGTT AGTGAATTAA AAAGCGTGCA GCCCTCTAAC
    CATGGCATCT ACCTTCCTTC GGACACCCAG GAGCATGCGG GATCTGGGAG GGCATCTTCT
    ATGCCACGTC TGACTGTGGA TCCCCAGGTG GTGACAGACC CTAGCTCCAT GAGACGTTCA
    TTTTCCACTA TTCGGGATAA GCGTTCAAAT TCCTCGTGGT TGGAGGAATT CTCCATGGAG
    CGAAGCAGTG AAAATACCTA CAAGTCCCGT CGCCGGAGTT ACCACTCCTC CTTGCGGCTG
    TCAGCCCACC GCCTGAACTC TGATTCAGGC CACAAGTCTG ACACTCACCG CTCAGGGGGC
    AGGGAGCGGG GACGATCAAA AGAGCGAAAG CATCTTCTCT CTCCTGATGT CTCCCGCTGC
    AATTCAGAAG AGCGAGGGAC CCAGGCTGAC TGGGAGTCCC CAGAGCGCCG TCAATCCAGG
    TCACCCAGTG AGGGCAGGTC ACAGACGCCC AACAGACAGG GCACAGGTTC CCTAAGTGAG
    AGCTCCATCC CCTCTGTCTC TGACACCAGC ACCCCAAGAA GAAGTCGTCG GCAGCTCCCA
    CCCGTCCCGC CAAAGCCCCG GCCCCTCCTT TCCTACAGCT CCCTGATTCG ACACGCGGGC
    AGCATCTCTC CACCTGCTGA TGGAAGCGAG GAGGGCTCCC CGCTGACCTC CCAAGCTCTG
    GAGAGCAACA ATGCTTGCCT GACCGAGTCT TCCAACTCTC CGCACCCCCA GCAGAGCCAA
    CATGCCTCCC CACAGCGCTA CATCTCCGAG CCCTACTTGG CCCTGCACGA AGACTCCCAC
    GCCTCAGACT GTGGTGAGGA GGAGACGCTC ACTTTCGAAG CAGCCGTGGC TACTAGCCTG
    GGCCGTTCCA ACACCATCGG CTCAGCCCCA CCCCTGCGGC ATAGCTGGCA GATGCCCAAC
    GGGCACTATC GGCGGCGGAG GCGCGGGGGG CCTGGGCCAG GCATGATGTG TGGGGCTGTC
    AACAACCTGC TAAGTGACAC GGAAGAAGAT GACAAATGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192222.1
    CDS196..7137
    Translation

    Target ORF information:

    RefSeq Version NM_001205293.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205293.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG
    CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT
    GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG
    TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC
    AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC
    GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT
    CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG
    GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG
    ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG
    AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA
    AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA
    GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA
    CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC
    AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG
    ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC
    TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC
    AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC
    AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG
    AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA
    GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT
    GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC
    ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT
    ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA
    GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCGATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCCGT GTGGGAGCAG
    CGTACCAGCC AGCTGAGGAA GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAGAGAG
    GAGGCGCCGA CCATGAACCC GCTCAACCCC CTCAACCCGC TCAGCTCCCT CAACCCGCTC
    AATGCCCACC CCAGCCTTTA TCGGCGACCC AGGGCCATTG AGGGCCTGGC CCTGGGCCTG
    GCCCTGGAGA AGTTCGAGGA GGAGCGCATC AGCCGTGGGG GGTCCCTCAA GGGGGATGGA
    GGGGACCGAT CCAGTGCCCT GGACAACCAG AGGACCCCTT TGTCCCTGGG CCAGCGGGAG
    CCACCATGGC TGGCCAGGCC CTGTCATGGA AACTGTGACC CGACTCAGCA GGAGGCAGGG
    GGAGGAGAGG CTGTGGTGAC CTTTGAGGAC CGGGCCAGGC ACAGGCAGAG CCAACGGCGC
    AGCCGGCATC GCCGCGTCAG GACAGAAGGC AAGGAGTCCT CTTCAGCCTC CCGGAGCAGG
    TCTGCCAGCC AGGAACGCAG TCTGGATGAA GCCATGCCCA CTGAAGGGGA GAAGGACCAT
    GAGCTCAGGG GCAACCATGG TGCCAAGGAG CCAACGATCC AAGAAGAGAG AGCCCAGGAT
    TTAAGGAGGA CCAACAGTCT GATGGTGTCC AGAGGCTCCG GGCTGGCAGG AGGCCTTGAT
    GAGGCTGACA CCCCCCTAGT CCTGCCCCAT CCTGAGCTGG AAGTGGGGAA GCACGTGGTG
    CTGACGGAGC AGGAGCCAGA AGGCAGCAGT GAGCAGGCCC TGCTGGGGAA TGTGCAGCTA
    GACATGGGCC GGGTCATCAG CCAGAGCGAG CCTGACCTCT CCTGCATCAC GGCCAACACG
    GACAAGGCCA CCACCGAGAG CACCAGCGTC ACCGTCGCCA TCCCCGACGT GGACCCCTTG
    GTGGACTCAA CCGTGGTGCA CATTAGCAAC AAGACGGATG GGGAAGCCAG TCCCTTGAAG
    GAGGCAGAGA TCAGAGAGGA TGAGGAGGAG GTGGAGAAGA AGAAGCAGAA GAAGGAGAAG
    CGTGAGACAG GCAAAGCCAT GGTGCCCCAC AGCTCAATGT TCATCTTCAG CACCACCAAC
    CCGATCCGGA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATCCTC
    CTGGTGATTG CAGCCAGCAG CATCGCCCTG GCGGCAGAGG ACCCCGTCCT GACCAACTCG
    GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTGTTCA CGGGCGTGTT CACCTTTGAG
    ATGGTTATAA AGATGATAGA CCAAGGCTTG ATCCTGCAGG ATGGGTCCTA CTTCCGAGAC
    TTGTGGAACA TCCTGGACTT TGTGGTGGTC GTTGGCGCAT TGGTGGCCTT TGCTCTGGCG
    AACGCTTTGG GAACCAACAA AGGACGGGAC ATCAAGACCA TCAAGTCTCT GCGGGTGCTC
    CGAGTTCTAA GGCCACTGAA AACCATCAAG CGCTTGCCCA AGCTCAAGGC CGTCTTCGAC
    TGCGTAGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TTGTGTACAA GCTCTTCATG
    TTCATCTTTG CTGTCATCGC AGTTCAGCTC TTCAAGGGAA AGTTCTTTTA TTGCACGGAC
    AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTATG TAGATCATGA GAAAAACAAG
    ATGGAGGTGA AGGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG
    GCCCTGCTGA CCCTCTTCAC CGTCTCCACA GGGGAAGGAT GGCCTCAAGT TCTGCAGCAC
    TCTGTAGATG TGACAGAGGA AGACCGAGGC CCAAGCCGCA GCAACCGCAT GGAGATGTCT
    ATCTTTTATG TAGTCTACTT TGTGGTCTTC CCCTTCTTCT TTGTCAATAT CTTTGTGGCT
    CTCATCATCA TCACCTTCCA GGAGCAAGGG GATAAGATGA TGGAGGAGTG CAGCCTGGAG
    AAGAATGAGA GGGCGTGCAT CGACTTCGCC ATCAGCGCCA AACCTCTCAC CCGCTACATG
    CCGCAGAACA GACACACCTT CCAGTACCGC GTGTGGCACT TTGTGGTGTC TCCGTCCTTT
    GAGTACACCA TTATGGCCAT GATCGCCTTG AATACTGTTG TGCTGATGAT GAAGTATTAT
    TCTGCTCCCT GTACCTATGA GCTGGCCCTG AAGTACCTGA ATATCGCCTT CACCATGGTG
    TTTTCCCTGG AATGTGTCCT GAAGGTCATC GCTTTTGGCT TTTTGAACTA TTTCCGAGAC
    ACCTGGAATA TCTTTGACTT CATCACCGTG ATTGGCAGTA TCACAGAAAT TATCCTGACA
    GACAGCAAGC TGGTGAACAC CAGTGGCTTC AATATGAGCT TTCTGAAGCT CTTCCGAGCT
    GCCCGCCTCA TAAAGCTCCT GCGTCAGGGC TATACCATAC GCATTTTGCT GTGGACCTTT
    GTGCAGTCCT TTAAGGCCCT CCCTTATGTC TGCCTTTTAA TTGCCATGCT TTTCTTCATT
    TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGACGAGGA GAGTCACATC
    AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTAA TGCTACTCTT CAGGAGTGCC
    ACAGGTGAGG CCTGGCAGGA GATTATGCTG TCATGCCTTG GGGAGAAGGG CTGTGAGCCT
    GACACCACCG CACCATCAGG GCAGAACGAG AACGAACGCT GCGGCACCGA TCTGGCCTAC
    GTGTACTTTG TCTCCTTCAT CTTCTTCTGC TCCTTCTTGA TGCTCAACCT GTTTGTGGCC
    GTCATCATGG ACAACTTTGA GTACCTGACT CGGGACTCCT CCATCCTGGG GCCTCACCAC
    TTGGACGAGT TTGTCCGCGT CTGGGCAGAA TATGACCGAG CAGCATGTGG CCGCATCCAT
    TACACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCTC CGCTAGGCCT CGGCAAGAGA
    TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTGATGA ACATGCCAGT AGCTGAGGAC
    ATGACGGTCC ACTTCACCTC CACACTTATG GCTCTGATCC GGACAGCTCT GGACATTAAA
    ATTGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCAG AGCTACAAAA GGAGACCCTA
    GCCATCTGGC CTCACCTATC CCAGAAGATG CTGGATCTGC TTGTGCCCAT GCCCAAAGCC
    TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAGCAG
    AGTAAGGTGA AGAAGCAGAG GCAGCAGCTG GAGGAACAGA AAAATGCCCC CATGTTCCAG
    CGCATGGAGC CTTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCTTAC
    CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGGAGTG GATACCCTTC GATGAGTCCA
    CTCTCTCCCC AGGATATATT CCAGTTGGCT TGTATGGACC CCGCCGATGA CGGACAGTTC
    CAAGAACGGC AGTCTCTGGA GCCAGAGGTT AGTGAATTAA AAAGCGTGCA GCCCTCTAAC
    CATGGCATCT ACCTTCCTTC GGACACCCAG GAGCATGCGG GATCTGGGAG GGCATCTTCT
    ATGCCACGTC TGACTGTGGA TCCCCAGGTG GTGACAGACC CTAGCTCCAT GAGACGTTCA
    TTTTCCACTA TTCGGGATAA GCGTTCAAAT TCCTCGTGGT TGGAGGAATT CTCCATGGAG
    CGAAGCAGTG AAAATACCTA CAAGTCCCGT CGCCGGAGTT ACCACTCCTC CTTGCGGCTG
    TCAGCCCACC GCCTGAACTC TGATTCAGGC CACAAGTCTG ACACTCACCG CTCAGGGGGC
    AGGGAGCGGG GACGATCAAA AGAGCGAAAG CATCTTCTCT CTCCTGATGT CTCCCGCTGC
    AATTCAGAAG AGCGAGGGAC CCAGGCTGAC TGGGAGTCCC CAGAGCGCCG TCAATCCAGG
    TCACCCAGTG AGGGCAGGTC ACAGACGCCC AACAGACAGG GCACAGGTTC CCTAAGTGAG
    AGCTCCATCC CCTCTGTCTC TGACACCAGC ACCCCAAGAA GAAGTCGTCG GCAGCTCCCA
    CCCGTCCCGC CAAAGCCCCG GCCCCTCCTT TCCTACAGCT CCCTGATTCG ACACGCGGGC
    AGCATCTCTC CACCTGCTGA TGGAAGCGAG GAGGGCTCCC CGCTGACCTC CCAAGCTCTG
    GAGAGCAACA ATGCTTGCCT GACCGAGTCT TCCAACTCTC CGCACCCCCA GCAGAGCCAA
    CATGCCTCCC CACAGCGCTA CATCTCCGAG CCCTACTTGG CCCTGCACGA AGACTCCCAC
    GCCTCAGACT GTGGTGAGGA GGAGACGCTC ACTTTCGAAG CAGCCGTGGC TACTAGCCTG
    GGCCGTTCCA ACACCATCGG CTCAGCCCCA CCCCTGCGGC ATAGCTGGCA GATGCCCAAC
    GGGCACTATC GGCGGCGGAG GCGCGGGGGG CCTGGGCCAG GCATGATGTG TGGGGCTGTC
    AACAACCTGC TAAGTGACAC GGAAGAAGAT GACAAATGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11422
    Clone ID Related Accession (Same CDS sequence) NM_001205293.1 , NM_001205293.3
    Accession Version NM_001205293.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-21
    Organism Homo sapiens(human)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160059.7, BP230397.1, L27745.2, BP230632.1, AL161734.12 and KF510705.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001205293.2. Transcript Variant: This variant (1) represents the longest transcript, and encodes the longest isoform (1, also known as alpha-1E). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L27745.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000367573.7/ ENSP00000356545.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG
    CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT
    GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG
    TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC
    AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC
    GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT
    CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG
    GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG
    ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG
    AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA
    AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA
    GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA
    CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC
    AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG
    ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC
    TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC
    AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC
    AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG
    AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA
    GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT
    GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC
    ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT
    ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA
    GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCGATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCCGT GTGGGAGCAG
    CGTACCAGCC AGCTGAGGAA GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAGAGAG
    GAGGCGCCGA CCATGAACCC GCTCAACCCC CTCAACCCGC TCAGCTCCCT CAACCCGCTC
    AATGCCCACC CCAGCCTTTA TCGGCGACCC AGGGCCATTG AGGGCCTGGC CCTGGGCCTG
    GCCCTGGAGA AGTTCGAGGA GGAGCGCATC AGCCGTGGGG GGTCCCTCAA GGGGGATGGA
    GGGGACCGAT CCAGTGCCCT GGACAACCAG AGGACCCCTT TGTCCCTGGG CCAGCGGGAG
    CCACCATGGC TGGCCAGGCC CTGTCATGGA AACTGTGACC CGACTCAGCA GGAGGCAGGG
    GGAGGAGAGG CTGTGGTGAC CTTTGAGGAC CGGGCCAGGC ACAGGCAGAG CCAACGGCGC
    AGCCGGCATC GCCGCGTCAG GACAGAAGGC AAGGAGTCCT CTTCAGCCTC CCGGAGCAGG
    TCTGCCAGCC AGGAACGCAG TCTGGATGAA GCCATGCCCA CTGAAGGGGA GAAGGACCAT
    GAGCTCAGGG GCAACCATGG TGCCAAGGAG CCAACGATCC AAGAAGAGAG AGCCCAGGAT
    TTAAGGAGGA CCAACAGTCT GATGGTGTCC AGAGGCTCCG GGCTGGCAGG AGGCCTTGAT
    GAGGCTGACA CCCCCCTAGT CCTGCCCCAT CCTGAGCTGG AAGTGGGGAA GCACGTGGTG
    CTGACGGAGC AGGAGCCAGA AGGCAGCAGT GAGCAGGCCC TGCTGGGGAA TGTGCAGCTA
    GACATGGGCC GGGTCATCAG CCAGAGCGAG CCTGACCTCT CCTGCATCAC GGCCAACACG
    GACAAGGCCA CCACCGAGAG CACCAGCGTC ACCGTCGCCA TCCCCGACGT GGACCCCTTG
    GTGGACTCAA CCGTGGTGCA CATTAGCAAC AAGACGGATG GGGAAGCCAG TCCCTTGAAG
    GAGGCAGAGA TCAGAGAGGA TGAGGAGGAG GTGGAGAAGA AGAAGCAGAA GAAGGAGAAG
    CGTGAGACAG GCAAAGCCAT GGTGCCCCAC AGCTCAATGT TCATCTTCAG CACCACCAAC
    CCGATCCGGA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATCCTC
    CTGGTGATTG CAGCCAGCAG CATCGCCCTG GCGGCAGAGG ACCCCGTCCT GACCAACTCG
    GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTGTTCA CGGGCGTGTT CACCTTTGAG
    ATGGTTATAA AGATGATAGA CCAAGGCTTG ATCCTGCAGG ATGGGTCCTA CTTCCGAGAC
    TTGTGGAACA TCCTGGACTT TGTGGTGGTC GTTGGCGCAT TGGTGGCCTT TGCTCTGGCG
    AACGCTTTGG GAACCAACAA AGGACGGGAC ATCAAGACCA TCAAGTCTCT GCGGGTGCTC
    CGAGTTCTAA GGCCACTGAA AACCATCAAG CGCTTGCCCA AGCTCAAGGC CGTCTTCGAC
    TGCGTAGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TTGTGTACAA GCTCTTCATG
    TTCATCTTTG CTGTCATCGC AGTTCAGCTC TTCAAGGGAA AGTTCTTTTA TTGCACGGAC
    AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTATG TAGATCATGA GAAAAACAAG
    ATGGAGGTGA AGGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG
    GCCCTGCTGA CCCTCTTCAC CGTCTCCACA GGGGAAGGAT GGCCTCAAGT TCTGCAGCAC
    TCTGTAGATG TGACAGAGGA AGACCGAGGC CCAAGCCGCA GCAACCGCAT GGAGATGTCT
    ATCTTTTATG TAGTCTACTT TGTGGTCTTC CCCTTCTTCT TTGTCAATAT CTTTGTGGCT
    CTCATCATCA TCACCTTCCA GGAGCAAGGG GATAAGATGA TGGAGGAGTG CAGCCTGGAG
    AAGAATGAGA GGGCGTGCAT CGACTTCGCC ATCAGCGCCA AACCTCTCAC CCGCTACATG
    CCGCAGAACA GACACACCTT CCAGTACCGC GTGTGGCACT TTGTGGTGTC TCCGTCCTTT
    GAGTACACCA TTATGGCCAT GATCGCCTTG AATACTGTTG TGCTGATGAT GAAGTATTAT
    TCTGCTCCCT GTACCTATGA GCTGGCCCTG AAGTACCTGA ATATCGCCTT CACCATGGTG
    TTTTCCCTGG AATGTGTCCT GAAGGTCATC GCTTTTGGCT TTTTGAACTA TTTCCGAGAC
    ACCTGGAATA TCTTTGACTT CATCACCGTG ATTGGCAGTA TCACAGAAAT TATCCTGACA
    GACAGCAAGC TGGTGAACAC CAGTGGCTTC AATATGAGCT TTCTGAAGCT CTTCCGAGCT
    GCCCGCCTCA TAAAGCTCCT GCGTCAGGGC TATACCATAC GCATTTTGCT GTGGACCTTT
    GTGCAGTCCT TTAAGGCCCT CCCTTATGTC TGCCTTTTAA TTGCCATGCT TTTCTTCATT
    TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGACGAGGA GAGTCACATC
    AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTAA TGCTACTCTT CAGGAGTGCC
    ACAGGTGAGG CCTGGCAGGA GATTATGCTG TCATGCCTTG GGGAGAAGGG CTGTGAGCCT
    GACACCACCG CACCATCAGG GCAGAACGAG AACGAACGCT GCGGCACCGA TCTGGCCTAC
    GTGTACTTTG TCTCCTTCAT CTTCTTCTGC TCCTTCTTGA TGCTCAACCT GTTTGTGGCC
    GTCATCATGG ACAACTTTGA GTACCTGACT CGGGACTCCT CCATCCTGGG GCCTCACCAC
    TTGGACGAGT TTGTCCGCGT CTGGGCAGAA TATGACCGAG CAGCATGTGG CCGCATCCAT
    TACACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCTC CGCTAGGCCT CGGCAAGAGA
    TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTGATGA ACATGCCAGT AGCTGAGGAC
    ATGACGGTCC ACTTCACCTC CACACTTATG GCTCTGATCC GGACAGCTCT GGACATTAAA
    ATTGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCAG AGCTACAAAA GGAGACCCTA
    GCCATCTGGC CTCACCTATC CCAGAAGATG CTGGATCTGC TTGTGCCCAT GCCCAAAGCC
    TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAGCAG
    AGTAAGGTGA AGAAGCAGAG GCAGCAGCTG GAGGAACAGA AAAATGCCCC CATGTTCCAG
    CGCATGGAGC CTTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCTTAC
    CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGGAGTG GATACCCTTC GATGAGTCCA
    CTCTCTCCCC AGGATATATT CCAGTTGGCT TGTATGGACC CCGCCGATGA CGGACAGTTC
    CAAGAACGGC AGTCTCTGGA GCCAGAGGTT AGTGAATTAA AAAGCGTGCA GCCCTCTAAC
    CATGGCATCT ACCTTCCTTC GGACACCCAG GAGCATGCGG GATCTGGGAG GGCATCTTCT
    ATGCCACGTC TGACTGTGGA TCCCCAGGTG GTGACAGACC CTAGCTCCAT GAGACGTTCA
    TTTTCCACTA TTCGGGATAA GCGTTCAAAT TCCTCGTGGT TGGAGGAATT CTCCATGGAG
    CGAAGCAGTG AAAATACCTA CAAGTCCCGT CGCCGGAGTT ACCACTCCTC CTTGCGGCTG
    TCAGCCCACC GCCTGAACTC TGATTCAGGC CACAAGTCTG ACACTCACCG CTCAGGGGGC
    AGGGAGCGGG GACGATCAAA AGAGCGAAAG CATCTTCTCT CTCCTGATGT CTCCCGCTGC
    AATTCAGAAG AGCGAGGGAC CCAGGCTGAC TGGGAGTCCC CAGAGCGCCG TCAATCCAGG
    TCACCCAGTG AGGGCAGGTC ACAGACGCCC AACAGACAGG GCACAGGTTC CCTAAGTGAG
    AGCTCCATCC CCTCTGTCTC TGACACCAGC ACCCCAAGAA GAAGTCGTCG GCAGCTCCCA
    CCCGTCCCGC CAAAGCCCCG GCCCCTCCTT TCCTACAGCT CCCTGATTCG ACACGCGGGC
    AGCATCTCTC CACCTGCTGA TGGAAGCGAG GAGGGCTCCC CGCTGACCTC CCAAGCTCTG
    GAGAGCAACA ATGCTTGCCT GACCGAGTCT TCCAACTCTC CGCACCCCCA GCAGAGCCAA
    CATGCCTCCC CACAGCGCTA CATCTCCGAG CCCTACTTGG CCCTGCACGA AGACTCCCAC
    GCCTCAGACT GTGGTGAGGA GGAGACGCTC ACTTTCGAAG CAGCCGTGGC TACTAGCCTG
    GGCCGTTCCA ACACCATCGG CTCAGCCCCA CCCCTGCGGC ATAGCTGGCA GATGCCCAAC
    GGGCACTATC GGCGGCGGAG GCGCGGGGGG CCTGGGCCAG GCATGATGTG TGGGGCTGTC
    AACAACCTGC TAAGTGACAC GGAAGAAGAT GACAAATGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192222.1
    CDS229..7170
    Translation

    Target ORF information:

    RefSeq Version NM_001205293.3
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205293.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGCC AGGCCAGGGT CCGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCGG GGCAGGCGGC CGCCTACAAG
    CAGACGAAAG CACAGAGGGC GCGGACTATG GCTTTGTACA ACCCCATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TATGCCAAGA AGCTCATCGA TTGGCCGCCA TTTGAGTACA TGATCCTGGC CACCATCATT
    GCCAACTGCA TCGTCCTGGC CCTGGAGCAG CATCTTCCTG AGGATGACAA GACCCCCATG
    TCCCGAAGAC TGGAGAAGAC AGAACCTTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATTG TGGCCCTGGG GTTCATCTTC CATAAGGGCT CTTACCTCCG CAATGGCTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CCACTGCAGG AACCCACTTC
    AATACTCACG TGGACCTGAG GACCCTCCGG GCTGTGCGTG TCCTGCGGCC TTTGAAGCTC
    GTGTCAGGGA TACCTAGCCT GCAGATTGTG TTGAAGTCCA TCATGAAGGC CATGGTACCT
    CTTCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATTGGTTTG
    GAGTTCTACA GTGGCAAGTT ACATCGAGCA TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGTGTG CAGGGCTGCC CAGCTGGTTA TGAATGCAAG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCTGTGCTG
    ACTGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTAGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GAAGGGCTTT CATGAAGCTG CGGCGCCAGC AGCAGATTGA GCGTGAGCTG
    AATGGCTACC GTGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTCGCTGA AGAAAATAAA
    AATGCTGGAA CATCCGCCTT AGAAGTGCTT CGAAGGGCAA CCATCAAGAG GAGCCGGACA
    GAGGCCATGA CTCGAGACTC CAGTGATGAG CACTGTGTTG ATATCTCCTC TGTGGGCACA
    CCTCTGGCCC GAGCCAGTAT CAAAAGTGCA AAGGTAGACG GGGTCTCTTA TTTCCGGCAC
    AAGGAAAGGC TTCTGCGCAT CTCCATTCGC CACATGGTTA AATCCCAGGT GTTTTACTGG
    ATTGTGCTGA GCCTTGTGGC ACTCAACACT GCCTGTGTGG CCATTGTCCA TCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAATTTC TGTTTCTGGG ACTCTTCCTC
    TTGGAGATGT CCCTGAAGAT GTATGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCATTC
    AACTGCTTTG ATTTTGGGGT CACAGTGGGC AGTATCTTTG AAGTGGTCTG GGCAATCTTC
    AGACCTGGTA CGTCTTTTGG AATCAGTGTC TTGCGAGCCC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC TTCCCTACGG AATTTGGTGG TCTCCTTGAT GAGCTCAATG
    AAGTCTATCA TCAGTTTGCT TTTCCTCCTC TTCCTCTTCA TCGTTGTCTT TGCTCTCCTA
    GGAATGCAGT TATTTGGAGG CAGGTTTAAC TTTAATGATG GGACTCCTTC GGCAAATTTT
    GATACCTTCC CTGCAGCCAT CATGACTGTG TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAATGGGAT CCGCTCCCAG GGTGGGGTCA GCTCAGGCAT GTGGTCTGCC
    ATCTACTTCA TTGTGCTCAC CTTGTTTGGC AACTACACGC TACTGAATGT GTTCTTGGCT
    ATCGCTGTGG ATAATCTCGC CAACGCCCAG GAACTGACCA AGGATGAACA GGAGGAAGAA
    GAGGCCTTCA ACCAGAAACA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCGATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCACC ACATGTCCGT GTGGGAGCAG
    CGTACCAGCC AGCTGAGGAA GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAGAGAG
    GAGGCGCCGA CCATGAACCC GCTCAACCCC CTCAACCCGC TCAGCTCCCT CAACCCGCTC
    AATGCCCACC CCAGCCTTTA TCGGCGACCC AGGGCCATTG AGGGCCTGGC CCTGGGCCTG
    GCCCTGGAGA AGTTCGAGGA GGAGCGCATC AGCCGTGGGG GGTCCCTCAA GGGGGATGGA
    GGGGACCGAT CCAGTGCCCT GGACAACCAG AGGACCCCTT TGTCCCTGGG CCAGCGGGAG
    CCACCATGGC TGGCCAGGCC CTGTCATGGA AACTGTGACC CGACTCAGCA GGAGGCAGGG
    GGAGGAGAGG CTGTGGTGAC CTTTGAGGAC CGGGCCAGGC ACAGGCAGAG CCAACGGCGC
    AGCCGGCATC GCCGCGTCAG GACAGAAGGC AAGGAGTCCT CTTCAGCCTC CCGGAGCAGG
    TCTGCCAGCC AGGAACGCAG TCTGGATGAA GCCATGCCCA CTGAAGGGGA GAAGGACCAT
    GAGCTCAGGG GCAACCATGG TGCCAAGGAG CCAACGATCC AAGAAGAGAG AGCCCAGGAT
    TTAAGGAGGA CCAACAGTCT GATGGTGTCC AGAGGCTCCG GGCTGGCAGG AGGCCTTGAT
    GAGGCTGACA CCCCCCTAGT CCTGCCCCAT CCTGAGCTGG AAGTGGGGAA GCACGTGGTG
    CTGACGGAGC AGGAGCCAGA AGGCAGCAGT GAGCAGGCCC TGCTGGGGAA TGTGCAGCTA
    GACATGGGCC GGGTCATCAG CCAGAGCGAG CCTGACCTCT CCTGCATCAC GGCCAACACG
    GACAAGGCCA CCACCGAGAG CACCAGCGTC ACCGTCGCCA TCCCCGACGT GGACCCCTTG
    GTGGACTCAA CCGTGGTGCA CATTAGCAAC AAGACGGATG GGGAAGCCAG TCCCTTGAAG
    GAGGCAGAGA TCAGAGAGGA TGAGGAGGAG GTGGAGAAGA AGAAGCAGAA GAAGGAGAAG
    CGTGAGACAG GCAAAGCCAT GGTGCCCCAC AGCTCAATGT TCATCTTCAG CACCACCAAC
    CCGATCCGGA GGGCCTGCCA CTACATCGTG AACCTGCGCT ACTTTGAGAT GTGCATCCTC
    CTGGTGATTG CAGCCAGCAG CATCGCCCTG GCGGCAGAGG ACCCCGTCCT GACCAACTCG
    GAGCGCAACA AAGTCCTGAG GTATTTTGAC TATGTGTTCA CGGGCGTGTT CACCTTTGAG
    ATGGTTATAA AGATGATAGA CCAAGGCTTG ATCCTGCAGG ATGGGTCCTA CTTCCGAGAC
    TTGTGGAACA TCCTGGACTT TGTGGTGGTC GTTGGCGCAT TGGTGGCCTT TGCTCTGGCG
    AACGCTTTGG GAACCAACAA AGGACGGGAC ATCAAGACCA TCAAGTCTCT GCGGGTGCTC
    CGAGTTCTAA GGCCACTGAA AACCATCAAG CGCTTGCCCA AGCTCAAGGC CGTCTTCGAC
    TGCGTAGTGA CCTCCTTGAA GAATGTCTTC AACATACTCA TTGTGTACAA GCTCTTCATG
    TTCATCTTTG CTGTCATCGC AGTTCAGCTC TTCAAGGGAA AGTTCTTTTA TTGCACGGAC
    AGTTCCAAGG ACACAGAGAA GGAGTGCATA GGCAACTATG TAGATCATGA GAAAAACAAG
    ATGGAGGTGA AGGGCCGGGA ATGGAAGCGC CATGAATTCC ACTACGACAA CATTATCTGG
    GCCCTGCTGA CCCTCTTCAC CGTCTCCACA GGGGAAGGAT GGCCTCAAGT TCTGCAGCAC
    TCTGTAGATG TGACAGAGGA AGACCGAGGC CCAAGCCGCA GCAACCGCAT GGAGATGTCT
    ATCTTTTATG TAGTCTACTT TGTGGTCTTC CCCTTCTTCT TTGTCAATAT CTTTGTGGCT
    CTCATCATCA TCACCTTCCA GGAGCAAGGG GATAAGATGA TGGAGGAGTG CAGCCTGGAG
    AAGAATGAGA GGGCGTGCAT CGACTTCGCC ATCAGCGCCA AACCTCTCAC CCGCTACATG
    CCGCAGAACA GACACACCTT CCAGTACCGC GTGTGGCACT TTGTGGTGTC TCCGTCCTTT
    GAGTACACCA TTATGGCCAT GATCGCCTTG AATACTGTTG TGCTGATGAT GAAGTATTAT
    TCTGCTCCCT GTACCTATGA GCTGGCCCTG AAGTACCTGA ATATCGCCTT CACCATGGTG
    TTTTCCCTGG AATGTGTCCT GAAGGTCATC GCTTTTGGCT TTTTGAACTA TTTCCGAGAC
    ACCTGGAATA TCTTTGACTT CATCACCGTG ATTGGCAGTA TCACAGAAAT TATCCTGACA
    GACAGCAAGC TGGTGAACAC CAGTGGCTTC AATATGAGCT TTCTGAAGCT CTTCCGAGCT
    GCCCGCCTCA TAAAGCTCCT GCGTCAGGGC TATACCATAC GCATTTTGCT GTGGACCTTT
    GTGCAGTCCT TTAAGGCCCT CCCTTATGTC TGCCTTTTAA TTGCCATGCT TTTCTTCATT
    TATGCCATCA TTGGGATGCA GGTATTTGGA AACATAAAAT TAGACGAGGA GAGTCACATC
    AACCGGCACA ACAACTTCCG GAGTTTCTTT GGGTCCCTAA TGCTACTCTT CAGGAGTGCC
    ACAGGTGAGG CCTGGCAGGA GATTATGCTG TCATGCCTTG GGGAGAAGGG CTGTGAGCCT
    GACACCACCG CACCATCAGG GCAGAACGAG AACGAACGCT GCGGCACCGA TCTGGCCTAC
    GTGTACTTTG TCTCCTTCAT CTTCTTCTGC TCCTTCTTGA TGCTCAACCT GTTTGTGGCC
    GTCATCATGG ACAACTTTGA GTACCTGACT CGGGACTCCT CCATCCTGGG GCCTCACCAC
    TTGGACGAGT TTGTCCGCGT CTGGGCAGAA TATGACCGAG CAGCATGTGG CCGCATCCAT
    TACACTGAGA TGTATGAAAT GCTGACTCTC ATGTCACCTC CGCTAGGCCT CGGCAAGAGA
    TGTCCCTCCA AAGTGGCATA TAAGAGGTTG GTCCTGATGA ACATGCCAGT AGCTGAGGAC
    ATGACGGTCC ACTTCACCTC CACACTTATG GCTCTGATCC GGACAGCTCT GGACATTAAA
    ATTGCCAAAG GTGGTGCAGA CAGGCAGCAG CTAGACTCAG AGCTACAAAA GGAGACCCTA
    GCCATCTGGC CTCACCTATC CCAGAAGATG CTGGATCTGC TTGTGCCCAT GCCCAAAGCC
    TCTGACCTGA CTGTGGGCAA AATCTATGCA GCAATGATGA TCATGGACTA CTATAAGCAG
    AGTAAGGTGA AGAAGCAGAG GCAGCAGCTG GAGGAACAGA AAAATGCCCC CATGTTCCAG
    CGCATGGAGC CTTCATCTCT GCCTCAGGAG ATCATTGCTA ATGCCAAAGC CCTGCCTTAC
    CTCCAGCAGG ACCCCGTTTC AGGCCTGAGT GGCCGGAGTG GATACCCTTC GATGAGTCCA
    CTCTCTCCCC AGGATATATT CCAGTTGGCT TGTATGGACC CCGCCGATGA CGGACAGTTC
    CAAGAACGGC AGTCTCTGGA GCCAGAGGTT AGTGAATTAA AAAGCGTGCA GCCCTCTAAC
    CATGGCATCT ACCTTCCTTC GGACACCCAG GAGCATGCGG GATCTGGGAG GGCATCTTCT
    ATGCCACGTC TGACTGTGGA TCCCCAGGTG GTGACAGACC CTAGCTCCAT GAGACGTTCA
    TTTTCCACTA TTCGGGATAA GCGTTCAAAT TCCTCGTGGT TGGAGGAATT CTCCATGGAG
    CGAAGCAGTG AAAATACCTA CAAGTCCCGT CGCCGGAGTT ACCACTCCTC CTTGCGGCTG
    TCAGCCCACC GCCTGAACTC TGATTCAGGC CACAAGTCTG ACACTCACCG CTCAGGGGGC
    AGGGAGCGGG GACGATCAAA AGAGCGAAAG CATCTTCTCT CTCCTGATGT CTCCCGCTGC
    AATTCAGAAG AGCGAGGGAC CCAGGCTGAC TGGGAGTCCC CAGAGCGCCG TCAATCCAGG
    TCACCCAGTG AGGGCAGGTC ACAGACGCCC AACAGACAGG GCACAGGTTC CCTAAGTGAG
    AGCTCCATCC CCTCTGTCTC TGACACCAGC ACCCCAAGAA GAAGTCGTCG GCAGCTCCCA
    CCCGTCCCGC CAAAGCCCCG GCCCCTCCTT TCCTACAGCT CCCTGATTCG ACACGCGGGC
    AGCATCTCTC CACCTGCTGA TGGAAGCGAG GAGGGCTCCC CGCTGACCTC CCAAGCTCTG
    GAGAGCAACA ATGCTTGCCT GACCGAGTCT TCCAACTCTC CGCACCCCCA GCAGAGCCAA
    CATGCCTCCC CACAGCGCTA CATCTCCGAG CCCTACTTGG CCCTGCACGA AGACTCCCAC
    GCCTCAGACT GTGGTGAGGA GGAGACGCTC ACTTTCGAAG CAGCCGTGGC TACTAGCCTG
    GGCCGTTCCA ACACCATCGG CTCAGCCCCA CCCCTGCGGC ATAGCTGGCA GATGCCCAAC
    GGGCACTATC GGCGGCGGAG GCGCGGGGGG CCTGGGCCAG GCATGATGTG TGGGGCTGTC
    AACAACCTGC TAAGTGACAC GGAAGAAGAT GACAAATGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90473
    Clone ID Related Accession (Same CDS sequence) XM_017002251.1 , XM_017002243.1
    Accession Version XM_017002251.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC
    ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG
    TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG
    GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC
    TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC
    CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC
    CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC
    CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT
    CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG
    CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA
    GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA
    GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA
    GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG
    GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG
    GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG
    GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC
    ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC
    GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA
    GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG
    CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC
    TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT
    GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC
    GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC
    GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG
    TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG
    GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG
    TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC
    AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG
    TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC
    TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT
    CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC
    GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT
    CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC
    CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC
    AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG
    GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT
    CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG
    GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG
    ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC
    GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG
    AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA
    GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG
    AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT
    TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GCCCTCCCTT ATGTCTGCCT TTTAATTGCC
    ATGCTTTTCT TCATTTATGC CATCATTGGG ATGCAGGTAT TTGGAAACAT AAAATTAGAC
    GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGGTC CCTAATGCTA
    CTCTTCAGGA GTGCCACAGG TGAGGCCTGG CAGGAGATTA TGCTGTCATG CCTTGGGGAG
    AAGGGCTGTG AGCCTGACAC CACCGCACCA TCAGGGCAGA ACGAGAACGA ACGCTGCGGC
    ACCGATCTGG CCTACGTGTA CTTTGTCTCC TTCATCTTCT TCTGCTCCTT CTTGATGCTC
    AACCTGTTTG TGGCCGTCAT CATGGACAAC TTTGAGTACC TGACTCGGGA CTCCTCCATC
    CTGGGGCCTC ACCACTTGGA CGAGTTTGTC CGCGTCTGGG CAGAATATGA CCGAGCAGCA
    TGTGGCCGCA TCCATTACAC TGAGATGTAT GAAATGCTGA CTCTCATGTC ACCTCCGCTA
    GGCCTCGGCA AGAGATGTCC CTCCAAAGTG GCATATAAGA GGTTGGTCCT GATGAACATG
    CCAGTAGCTG AGGACATGAC GGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA
    GCTCTGGACA TTAAAATTGC CAAAGGTGGT GCAGACAGGC AGCAGCTAGA CTCAGAGCTA
    CAAAAGGAGA CCCTAGCCAT CTGGCCTCAC CTATCCCAGA AGATGCTGGA TCTGCTTGTG
    CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAAATCT ATGCAGCAAT GATGATCATG
    GACTACTATA AGCAGAGTAA GGTGAAGAAG CAGAGGCAGC AGCTGGAGGA ACAGAAAAAT
    GCCCCCATGT TCCAGCGCAT GGAGCCTTCA TCTCTGCCTC AGGAGATCAT TGCTAATGCC
    AAAGCCCTGC CTTACCTCCA GCAGGACCCC GTTTCAGGCC TGAGTGGCCG GAGTGGATAC
    CCTTCGATGA GTCCACTCTC TCCCCAGGAT ATATTCCAGT TGGCTTGTAT GGACCCCGCC
    GATGACGGAC AGTTCCAAGA ACGGCAGTCT CTGGAGCCAG AGGTTAGTGA ATTAAAAAGC
    GTGCAGCCCT CTAACCATGG CATCTACCTT CCTTCGGACA CCCAGGAGCA TGCGGGATCT
    GGGAGGGCAT CTTCTATGCC ACGTCTGACT GTGGATCCCC AGGTGGTGAC AGACCCTAGC
    TCCATGAGAC GTTCATTTTC CACTATTCGG GATAAGCGTT CAAATTCCTC GTGGTTGGAG
    GAATTCTCCA TGGAGCGAAG CAGTGAAAAT ACCTACAAGT CCCGTCGCCG GAGTTACCAC
    TCCTCCTTGC GGCTGTCAGC CCACCGCCTG AACTCTGATT CAGGCCACAA GTCTGACACT
    CACCGCTCAG GGGGCAGGGA GCGGGGACGA TCAAAAGAGC GAAAGCATCT TCTCTCTCCT
    GATGTCTCCC GCTGCAATTC AGAAGAGCGA GGGACCCAGG CTGACTGGGA GTCCCCAGAG
    CGCCGTCAAT CCAGGTCACC CAGTGAGGGC AGGTCACAGA CGCCCAACAG ACAGGGCACA
    GGTTCCCTAA GTGAGAGCTC CATCCCCTCT GTCTCTGACA CCAGCACCCC AAGAAGAAGT
    CGTCGGCAGC TCCCACCCGT CCCGCCAAAG CCCCGGCCCC TCCTTTCCTA CAGCTCCCTG
    ATTCGACACG CGGGCAGCAT CTCTCCACCT GCTGATGGAA GCGAGGAGGG CTCCCCGCTG
    ACCTCCCAAG CTCTGGAGAG CAACAATGCT TGCCTGACCG AGTCTTCCAA CTCTCCGCAC
    CCCCAGCAGA GCCAACATGC CTCCCCACAG CGCTACATCT CCGAGCCCTA CTTGGCCCTG
    CACGAAGACT CCCACGCCTC AGACTGTGGT GAGGAGGAGA CGCTCACTTT CGAAGCAGCC
    GTGGCTACTA GCCTGGGCCG TTCCAACACC ATCGGCTCAG CCCCACCCCT GCGGCATAGC
    TGGCAGATGC CCAACGGGCA CTATCGGCGG CGGAGGCGCG GGGGGCCTGG GCCAGGCATG
    ATGTGTGGGG CTGTCAACAA CCTGCTAAGT GACACGGAAG AAGATGACAA ATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016857740.1
    CDS84..7460
    Translation

    Target ORF information:

    RefSeq Version XM_017002251.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002251.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC
    ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG
    TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG
    GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC
    TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC
    CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC
    CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC
    CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT
    CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG
    CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA
    GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA
    GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA
    GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG
    GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG
    GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG
    GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC
    ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC
    GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA
    GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG
    CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC
    TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT
    GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC
    GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC
    GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG
    TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG
    GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG
    TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC
    AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG
    TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC
    TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT
    CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC
    GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT
    CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC
    CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC
    AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG
    GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT
    CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG
    GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG
    ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC
    GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG
    AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA
    GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG
    AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT
    TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GCCCTCCCTT ATGTCTGCCT TTTAATTGCC
    ATGCTTTTCT TCATTTATGC CATCATTGGG ATGCAGGTAT TTGGAAACAT AAAATTAGAC
    GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGGTC CCTAATGCTA
    CTCTTCAGGA GTGCCACAGG TGAGGCCTGG CAGGAGATTA TGCTGTCATG CCTTGGGGAG
    AAGGGCTGTG AGCCTGACAC CACCGCACCA TCAGGGCAGA ACGAGAACGA ACGCTGCGGC
    ACCGATCTGG CCTACGTGTA CTTTGTCTCC TTCATCTTCT TCTGCTCCTT CTTGATGCTC
    AACCTGTTTG TGGCCGTCAT CATGGACAAC TTTGAGTACC TGACTCGGGA CTCCTCCATC
    CTGGGGCCTC ACCACTTGGA CGAGTTTGTC CGCGTCTGGG CAGAATATGA CCGAGCAGCA
    TGTGGCCGCA TCCATTACAC TGAGATGTAT GAAATGCTGA CTCTCATGTC ACCTCCGCTA
    GGCCTCGGCA AGAGATGTCC CTCCAAAGTG GCATATAAGA GGTTGGTCCT GATGAACATG
    CCAGTAGCTG AGGACATGAC GGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA
    GCTCTGGACA TTAAAATTGC CAAAGGTGGT GCAGACAGGC AGCAGCTAGA CTCAGAGCTA
    CAAAAGGAGA CCCTAGCCAT CTGGCCTCAC CTATCCCAGA AGATGCTGGA TCTGCTTGTG
    CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAAATCT ATGCAGCAAT GATGATCATG
    GACTACTATA AGCAGAGTAA GGTGAAGAAG CAGAGGCAGC AGCTGGAGGA ACAGAAAAAT
    GCCCCCATGT TCCAGCGCAT GGAGCCTTCA TCTCTGCCTC AGGAGATCAT TGCTAATGCC
    AAAGCCCTGC CTTACCTCCA GCAGGACCCC GTTTCAGGCC TGAGTGGCCG GAGTGGATAC
    CCTTCGATGA GTCCACTCTC TCCCCAGGAT ATATTCCAGT TGGCTTGTAT GGACCCCGCC
    GATGACGGAC AGTTCCAAGA ACGGCAGTCT CTGGAGCCAG AGGTTAGTGA ATTAAAAAGC
    GTGCAGCCCT CTAACCATGG CATCTACCTT CCTTCGGACA CCCAGGAGCA TGCGGGATCT
    GGGAGGGCAT CTTCTATGCC ACGTCTGACT GTGGATCCCC AGGTGGTGAC AGACCCTAGC
    TCCATGAGAC GTTCATTTTC CACTATTCGG GATAAGCGTT CAAATTCCTC GTGGTTGGAG
    GAATTCTCCA TGGAGCGAAG CAGTGAAAAT ACCTACAAGT CCCGTCGCCG GAGTTACCAC
    TCCTCCTTGC GGCTGTCAGC CCACCGCCTG AACTCTGATT CAGGCCACAA GTCTGACACT
    CACCGCTCAG GGGGCAGGGA GCGGGGACGA TCAAAAGAGC GAAAGCATCT TCTCTCTCCT
    GATGTCTCCC GCTGCAATTC AGAAGAGCGA GGGACCCAGG CTGACTGGGA GTCCCCAGAG
    CGCCGTCAAT CCAGGTCACC CAGTGAGGGC AGGTCACAGA CGCCCAACAG ACAGGGCACA
    GGTTCCCTAA GTGAGAGCTC CATCCCCTCT GTCTCTGACA CCAGCACCCC AAGAAGAAGT
    CGTCGGCAGC TCCCACCCGT CCCGCCAAAG CCCCGGCCCC TCCTTTCCTA CAGCTCCCTG
    ATTCGACACG CGGGCAGCAT CTCTCCACCT GCTGATGGAA GCGAGGAGGG CTCCCCGCTG
    ACCTCCCAAG CTCTGGAGAG CAACAATGCT TGCCTGACCG AGTCTTCCAA CTCTCCGCAC
    CCCCAGCAGA GCCAACATGC CTCCCCACAG CGCTACATCT CCGAGCCCTA CTTGGCCCTG
    CACGAAGACT CCCACGCCTC AGACTGTGGT GAGGAGGAGA CGCTCACTTT CGAAGCAGCC
    GTGGCTACTA GCCTGGGCCG TTCCAACACC ATCGGCTCAG CCCCACCCCT GCGGCATAGC
    TGGCAGATGC CCAACGGGCA CTATCGGCGG CGGAGGCGCG GGGGGCCTGG GCCAGGCATG
    ATGTGTGGGG CTGTCAACAA CCTGCTAAGT GACACGGAAG AAGATGACAA ATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90473
    Clone ID Related Accession (Same CDS sequence) XM_017002251.1 , XM_017002243.1
    Accession Version XM_017002243.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC
    ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG
    TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG
    GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC
    TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC
    CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC
    CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC
    CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT
    CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG
    CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA
    GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA
    GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA
    GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG
    GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG
    GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG
    GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC
    ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC
    GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA
    GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG
    CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC
    TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT
    GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC
    GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC
    GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG
    TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG
    GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG
    TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC
    AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG
    TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC
    TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT
    CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC
    GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT
    CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC
    CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC
    AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG
    GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT
    CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG
    GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG
    ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC
    GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG
    AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA
    GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG
    AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT
    TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GCCCTCCCTT ATGTCTGCCT TTTAATTGCC
    ATGCTTTTCT TCATTTATGC CATCATTGGG ATGCAGGTAT TTGGAAACAT AAAATTAGAC
    GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGGTC CCTAATGCTA
    CTCTTCAGGA GTGCCACAGG TGAGGCCTGG CAGGAGATTA TGCTGTCATG CCTTGGGGAG
    AAGGGCTGTG AGCCTGACAC CACCGCACCA TCAGGGCAGA ACGAGAACGA ACGCTGCGGC
    ACCGATCTGG CCTACGTGTA CTTTGTCTCC TTCATCTTCT TCTGCTCCTT CTTGATGCTC
    AACCTGTTTG TGGCCGTCAT CATGGACAAC TTTGAGTACC TGACTCGGGA CTCCTCCATC
    CTGGGGCCTC ACCACTTGGA CGAGTTTGTC CGCGTCTGGG CAGAATATGA CCGAGCAGCA
    TGTGGCCGCA TCCATTACAC TGAGATGTAT GAAATGCTGA CTCTCATGTC ACCTCCGCTA
    GGCCTCGGCA AGAGATGTCC CTCCAAAGTG GCATATAAGA GGTTGGTCCT GATGAACATG
    CCAGTAGCTG AGGACATGAC GGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA
    GCTCTGGACA TTAAAATTGC CAAAGGTGGT GCAGACAGGC AGCAGCTAGA CTCAGAGCTA
    CAAAAGGAGA CCCTAGCCAT CTGGCCTCAC CTATCCCAGA AGATGCTGGA TCTGCTTGTG
    CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAAATCT ATGCAGCAAT GATGATCATG
    GACTACTATA AGCAGAGTAA GGTGAAGAAG CAGAGGCAGC AGCTGGAGGA ACAGAAAAAT
    GCCCCCATGT TCCAGCGCAT GGAGCCTTCA TCTCTGCCTC AGGAGATCAT TGCTAATGCC
    AAAGCCCTGC CTTACCTCCA GCAGGACCCC GTTTCAGGCC TGAGTGGCCG GAGTGGATAC
    CCTTCGATGA GTCCACTCTC TCCCCAGGAT ATATTCCAGT TGGCTTGTAT GGACCCCGCC
    GATGACGGAC AGTTCCAAGA ACGGCAGTCT CTGGAGCCAG AGGTTAGTGA ATTAAAAAGC
    GTGCAGCCCT CTAACCATGG CATCTACCTT CCTTCGGACA CCCAGGAGCA TGCGGGATCT
    GGGAGGGCAT CTTCTATGCC ACGTCTGACT GTGGATCCCC AGGTGGTGAC AGACCCTAGC
    TCCATGAGAC GTTCATTTTC CACTATTCGG GATAAGCGTT CAAATTCCTC GTGGTTGGAG
    GAATTCTCCA TGGAGCGAAG CAGTGAAAAT ACCTACAAGT CCCGTCGCCG GAGTTACCAC
    TCCTCCTTGC GGCTGTCAGC CCACCGCCTG AACTCTGATT CAGGCCACAA GTCTGACACT
    CACCGCTCAG GGGGCAGGGA GCGGGGACGA TCAAAAGAGC GAAAGCATCT TCTCTCTCCT
    GATGTCTCCC GCTGCAATTC AGAAGAGCGA GGGACCCAGG CTGACTGGGA GTCCCCAGAG
    CGCCGTCAAT CCAGGTCACC CAGTGAGGGC AGGTCACAGA CGCCCAACAG ACAGGGCACA
    GGTTCCCTAA GTGAGAGCTC CATCCCCTCT GTCTCTGACA CCAGCACCCC AAGAAGAAGT
    CGTCGGCAGC TCCCACCCGT CCCGCCAAAG CCCCGGCCCC TCCTTTCCTA CAGCTCCCTG
    ATTCGACACG CGGGCAGCAT CTCTCCACCT GCTGATGGAA GCGAGGAGGG CTCCCCGCTG
    ACCTCCCAAG CTCTGGAGAG CAACAATGCT TGCCTGACCG AGTCTTCCAA CTCTCCGCAC
    CCCCAGCAGA GCCAACATGC CTCCCCACAG CGCTACATCT CCGAGCCCTA CTTGGCCCTG
    CACGAAGACT CCCACGCCTC AGACTGTGGT GAGGAGGAGA CGCTCACTTT CGAAGCAGCC
    GTGGCTACTA GCCTGGGCCG TTCCAACACC ATCGGCTCAG CCCCACCCCT GCGGCATAGC
    TGGCAGATGC CCAACGGGCA CTATCGGCGG CGGAGGCGCG GGGGGCCTGG GCCAGGCATG
    ATGTGTGGGG CTGTCAACAA CCTGCTAAGT GACACGGAAG AAGATGACAA ATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016857732.1
    CDS426..7802
    Translation

    Target ORF information:

    RefSeq Version XM_017002243.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002243.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC
    ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG
    TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG
    GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC
    TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC
    CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC
    CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC
    CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT
    CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG
    CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA
    GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA
    GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA
    GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG
    GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG
    GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG
    GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC
    ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC
    GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA
    GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG
    CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC
    TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT
    GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC
    GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC
    GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG
    TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG
    GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG
    TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC
    AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG
    TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC
    TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT
    CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC
    GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT
    CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC
    CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC
    AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG
    GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT
    CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG
    GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG
    ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC
    GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG
    AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA
    GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG
    AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT
    TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GCCCTCCCTT ATGTCTGCCT TTTAATTGCC
    ATGCTTTTCT TCATTTATGC CATCATTGGG ATGCAGGTAT TTGGAAACAT AAAATTAGAC
    GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGGTC CCTAATGCTA
    CTCTTCAGGA GTGCCACAGG TGAGGCCTGG CAGGAGATTA TGCTGTCATG CCTTGGGGAG
    AAGGGCTGTG AGCCTGACAC CACCGCACCA TCAGGGCAGA ACGAGAACGA ACGCTGCGGC
    ACCGATCTGG CCTACGTGTA CTTTGTCTCC TTCATCTTCT TCTGCTCCTT CTTGATGCTC
    AACCTGTTTG TGGCCGTCAT CATGGACAAC TTTGAGTACC TGACTCGGGA CTCCTCCATC
    CTGGGGCCTC ACCACTTGGA CGAGTTTGTC CGCGTCTGGG CAGAATATGA CCGAGCAGCA
    TGTGGCCGCA TCCATTACAC TGAGATGTAT GAAATGCTGA CTCTCATGTC ACCTCCGCTA
    GGCCTCGGCA AGAGATGTCC CTCCAAAGTG GCATATAAGA GGTTGGTCCT GATGAACATG
    CCAGTAGCTG AGGACATGAC GGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA
    GCTCTGGACA TTAAAATTGC CAAAGGTGGT GCAGACAGGC AGCAGCTAGA CTCAGAGCTA
    CAAAAGGAGA CCCTAGCCAT CTGGCCTCAC CTATCCCAGA AGATGCTGGA TCTGCTTGTG
    CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAAATCT ATGCAGCAAT GATGATCATG
    GACTACTATA AGCAGAGTAA GGTGAAGAAG CAGAGGCAGC AGCTGGAGGA ACAGAAAAAT
    GCCCCCATGT TCCAGCGCAT GGAGCCTTCA TCTCTGCCTC AGGAGATCAT TGCTAATGCC
    AAAGCCCTGC CTTACCTCCA GCAGGACCCC GTTTCAGGCC TGAGTGGCCG GAGTGGATAC
    CCTTCGATGA GTCCACTCTC TCCCCAGGAT ATATTCCAGT TGGCTTGTAT GGACCCCGCC
    GATGACGGAC AGTTCCAAGA ACGGCAGTCT CTGGAGCCAG AGGTTAGTGA ATTAAAAAGC
    GTGCAGCCCT CTAACCATGG CATCTACCTT CCTTCGGACA CCCAGGAGCA TGCGGGATCT
    GGGAGGGCAT CTTCTATGCC ACGTCTGACT GTGGATCCCC AGGTGGTGAC AGACCCTAGC
    TCCATGAGAC GTTCATTTTC CACTATTCGG GATAAGCGTT CAAATTCCTC GTGGTTGGAG
    GAATTCTCCA TGGAGCGAAG CAGTGAAAAT ACCTACAAGT CCCGTCGCCG GAGTTACCAC
    TCCTCCTTGC GGCTGTCAGC CCACCGCCTG AACTCTGATT CAGGCCACAA GTCTGACACT
    CACCGCTCAG GGGGCAGGGA GCGGGGACGA TCAAAAGAGC GAAAGCATCT TCTCTCTCCT
    GATGTCTCCC GCTGCAATTC AGAAGAGCGA GGGACCCAGG CTGACTGGGA GTCCCCAGAG
    CGCCGTCAAT CCAGGTCACC CAGTGAGGGC AGGTCACAGA CGCCCAACAG ACAGGGCACA
    GGTTCCCTAA GTGAGAGCTC CATCCCCTCT GTCTCTGACA CCAGCACCCC AAGAAGAAGT
    CGTCGGCAGC TCCCACCCGT CCCGCCAAAG CCCCGGCCCC TCCTTTCCTA CAGCTCCCTG
    ATTCGACACG CGGGCAGCAT CTCTCCACCT GCTGATGGAA GCGAGGAGGG CTCCCCGCTG
    ACCTCCCAAG CTCTGGAGAG CAACAATGCT TGCCTGACCG AGTCTTCCAA CTCTCCGCAC
    CCCCAGCAGA GCCAACATGC CTCCCCACAG CGCTACATCT CCGAGCCCTA CTTGGCCCTG
    CACGAAGACT CCCACGCCTC AGACTGTGGT GAGGAGGAGA CGCTCACTTT CGAAGCAGCC
    GTGGCTACTA GCCTGGGCCG TTCCAACACC ATCGGCTCAG CCCCACCCCT GCGGCATAGC
    TGGCAGATGC CCAACGGGCA CTATCGGCGG CGGAGGCGCG GGGGGCCTGG GCCAGGCATG
    ATGTGTGGGG CTGTCAACAA CCTGCTAAGT GACACGGAAG AAGATGACAA ATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90474
    Clone ID Related Accession (Same CDS sequence) XM_017002244.1
    Accession Version XM_017002244.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC
    ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG
    TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG
    GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC
    TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC
    CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC
    CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC
    CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT
    CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG
    CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA
    GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA
    GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA
    GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG
    GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG
    GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG
    GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC
    ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC
    GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA
    GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG
    CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC
    TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT
    GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC
    GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC
    GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG
    TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG
    GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG
    TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC
    AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG
    TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC
    TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT
    CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC
    GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT
    CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC
    CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC
    AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG
    GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT
    CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG
    GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG
    ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC
    GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG
    AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA
    GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG
    AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT
    TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GCCCTCCCTT ATGTCTGCCT TTTAATTGCC
    ATGCTTTTCT TCATTTATGC CATCATTGGG ATGCAGGTAT TTGGAAACAT AAAATTAGAC
    GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGGTC CCTAATGCTA
    CTCTTCAGGA GTGCCACAGG TGAGGCCTGG CAGGAGATTA TGCTGTCATG CCTTGGGGAG
    AAGGGCTGTG AGCCTGACAC CACCGCACCA TCAGGGCAGA ACGAGAACGA ACGCTGCGGC
    ACCGATCTGG CCTACGTGTA CTTTGTCTCC TTCATCTTCT TCTGCTCCTT CTTGATGCTC
    AACCTGTTTG TGGCCGTCAT CATGGACAAC TTTGAGTACC TGACTCGGGA CTCCTCCATC
    CTGGGGCCTC ACCACTTGGA CGAGTTTGTC CGCGTCTGGG CAGAATATGA CCGAGCAGCA
    TGCGGCAGGA TCCCGTATAA GGATATGTAC AAATTAGTGC GGGTGATCTC GCCTCCTCTG
    GGCCTGGGAG AGAACTGCCC CTACAGGGTG GCTTGCAAGA GGTTGGTCCT GATGAACATG
    CCAGTAGCTG AGGACATGAC GGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA
    GCTCTGGACA TTAAAATTGC CAAAGGTGGT GCAGACAGGC AGCAGCTAGA CTCAGAGCTA
    CAAAAGGAGA CCCTAGCCAT CTGGCCTCAC CTATCCCAGA AGATGCTGGA TCTGCTTGTG
    CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAAATCT ATGCAGCAAT GATGATCATG
    GACTACTATA AGCAGAGTAA GGTGAAGAAG CAGAGGCAGC AGCTGGAGGA ACAGAAAAAT
    GCCCCCATGT TCCAGCGCAT GGAGCCTTCA TCTCTGCCTC AGGAGATCAT TGCTAATGCC
    AAAGCCCTGC CTTACCTCCA GCAGGACCCC GTTTCAGGCC TGAGTGGCCG GAGTGGATAC
    CCTTCGATGA GTCCACTCTC TCCCCAGGAT ATATTCCAGT TGGCTTGTAT GGACCCCGCC
    GATGACGGAC AGTTCCAAGA ACGGCAGTCT CTGGAGCCAG AGGTTAGTGA ATTAAAAAGC
    GTGCAGCCCT CTAACCATGG CATCTACCTT CCTTCGGACA CCCAGGAGCA TGCGGGATCT
    GGGAGGGCAT CTTCTATGCC ACGTCTGACT GTGGATCCCC AGGTGGTGAC AGACCCTAGC
    TCCATGAGAC GTTCATTTTC CACTATTCGG GATAAGCGTT CAAATTCCTC GTGGTTGGAG
    GAATTCTCCA TGGAGCGAAG CAGTGAAAAT ACCTACAAGT CCCGTCGCCG GAGTTACCAC
    TCCTCCTTGC GGCTGTCAGC CCACCGCCTG AACTCTGATT CAGGCCACAA GTCTGACACT
    CACCGCTCAG GGGGCAGGGA GCGGGGACGA TCAAAAGAGC GAAAGCATCT TCTCTCTCCT
    GATGTCTCCC GCTGCAATTC AGAAGAGCGA GGGACCCAGG CTGACTGGGA GTCCCCAGAG
    CGCCGTCAAT CCAGGTCACC CAGTGAGGGC AGGTCACAGA CGCCCAACAG ACAGGGCACA
    GGTTCCCTAA GTGAGAGCTC CATCCCCTCT GTCTCTGACA CCAGCACCCC AAGAAGAAGT
    CGTCGGCAGC TCCCACCCGT CCCGCCAAAG CCCCGGCCCC TCCTTTCCTA CAGCTCCCTG
    ATTCGACACG CGGGCAGCAT CTCTCCACCT GCTGATGGAA GCGAGGAGGG CTCCCCGCTG
    ACCTCCCAAG CTCTGGAGAG CAACAATGCT TGCCTGACCG AGTCTTCCAA CTCTCCGCAC
    CCCCAGCAGA GCCAACATGC CTCCCCACAG CGCTACATCT CCGAGCCCTA CTTGGCCCTG
    CACGAAGACT CCCACGCCTC AGACTGTGGT GAGGAGGAGA CGCTCACTTT CGAAGCAGCC
    GTGGCTACTA GCCTGGGCCG TTCCAACACC ATCGGCTCAG CCCCACCCCT GCGGCATAGC
    TGGCAGATGC CCAACGGGCA CTATCGGCGG CGGAGGCGCG GGGGGCCTGG GCCAGGCATG
    ATGTGTGGGG CTGTCAACAA CCTGCTAAGT GACACGGAAG AAGATGACAA ATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016857733.1
    CDS427..7803
    Translation

    Target ORF information:

    RefSeq Version XM_017002244.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002244.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC
    ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG
    TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG
    GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC
    TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC
    CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC
    CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC
    CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT
    CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG
    CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA
    GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA
    GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA
    GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG
    GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG
    GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG
    GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC
    ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC
    GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA
    GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG
    CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC
    TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT
    GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC
    GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC
    GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG
    TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG
    GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG
    TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC
    AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG
    TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC
    TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT
    CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC
    GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT
    CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC
    CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC
    AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG
    GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT
    CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG
    GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG
    ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC
    GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG
    AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA
    GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG
    AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT
    TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GCCCTCCCTT ATGTCTGCCT TTTAATTGCC
    ATGCTTTTCT TCATTTATGC CATCATTGGG ATGCAGGTAT TTGGAAACAT AAAATTAGAC
    GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGGTC CCTAATGCTA
    CTCTTCAGGA GTGCCACAGG TGAGGCCTGG CAGGAGATTA TGCTGTCATG CCTTGGGGAG
    AAGGGCTGTG AGCCTGACAC CACCGCACCA TCAGGGCAGA ACGAGAACGA ACGCTGCGGC
    ACCGATCTGG CCTACGTGTA CTTTGTCTCC TTCATCTTCT TCTGCTCCTT CTTGATGCTC
    AACCTGTTTG TGGCCGTCAT CATGGACAAC TTTGAGTACC TGACTCGGGA CTCCTCCATC
    CTGGGGCCTC ACCACTTGGA CGAGTTTGTC CGCGTCTGGG CAGAATATGA CCGAGCAGCA
    TGCGGCAGGA TCCCGTATAA GGATATGTAC AAATTAGTGC GGGTGATCTC GCCTCCTCTG
    GGCCTGGGAG AGAACTGCCC CTACAGGGTG GCTTGCAAGA GGTTGGTCCT GATGAACATG
    CCAGTAGCTG AGGACATGAC GGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA
    GCTCTGGACA TTAAAATTGC CAAAGGTGGT GCAGACAGGC AGCAGCTAGA CTCAGAGCTA
    CAAAAGGAGA CCCTAGCCAT CTGGCCTCAC CTATCCCAGA AGATGCTGGA TCTGCTTGTG
    CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAAATCT ATGCAGCAAT GATGATCATG
    GACTACTATA AGCAGAGTAA GGTGAAGAAG CAGAGGCAGC AGCTGGAGGA ACAGAAAAAT
    GCCCCCATGT TCCAGCGCAT GGAGCCTTCA TCTCTGCCTC AGGAGATCAT TGCTAATGCC
    AAAGCCCTGC CTTACCTCCA GCAGGACCCC GTTTCAGGCC TGAGTGGCCG GAGTGGATAC
    CCTTCGATGA GTCCACTCTC TCCCCAGGAT ATATTCCAGT TGGCTTGTAT GGACCCCGCC
    GATGACGGAC AGTTCCAAGA ACGGCAGTCT CTGGAGCCAG AGGTTAGTGA ATTAAAAAGC
    GTGCAGCCCT CTAACCATGG CATCTACCTT CCTTCGGACA CCCAGGAGCA TGCGGGATCT
    GGGAGGGCAT CTTCTATGCC ACGTCTGACT GTGGATCCCC AGGTGGTGAC AGACCCTAGC
    TCCATGAGAC GTTCATTTTC CACTATTCGG GATAAGCGTT CAAATTCCTC GTGGTTGGAG
    GAATTCTCCA TGGAGCGAAG CAGTGAAAAT ACCTACAAGT CCCGTCGCCG GAGTTACCAC
    TCCTCCTTGC GGCTGTCAGC CCACCGCCTG AACTCTGATT CAGGCCACAA GTCTGACACT
    CACCGCTCAG GGGGCAGGGA GCGGGGACGA TCAAAAGAGC GAAAGCATCT TCTCTCTCCT
    GATGTCTCCC GCTGCAATTC AGAAGAGCGA GGGACCCAGG CTGACTGGGA GTCCCCAGAG
    CGCCGTCAAT CCAGGTCACC CAGTGAGGGC AGGTCACAGA CGCCCAACAG ACAGGGCACA
    GGTTCCCTAA GTGAGAGCTC CATCCCCTCT GTCTCTGACA CCAGCACCCC AAGAAGAAGT
    CGTCGGCAGC TCCCACCCGT CCCGCCAAAG CCCCGGCCCC TCCTTTCCTA CAGCTCCCTG
    ATTCGACACG CGGGCAGCAT CTCTCCACCT GCTGATGGAA GCGAGGAGGG CTCCCCGCTG
    ACCTCCCAAG CTCTGGAGAG CAACAATGCT TGCCTGACCG AGTCTTCCAA CTCTCCGCAC
    CCCCAGCAGA GCCAACATGC CTCCCCACAG CGCTACATCT CCGAGCCCTA CTTGGCCCTG
    CACGAAGACT CCCACGCCTC AGACTGTGGT GAGGAGGAGA CGCTCACTTT CGAAGCAGCC
    GTGGCTACTA GCCTGGGCCG TTCCAACACC ATCGGCTCAG CCCCACCCCT GCGGCATAGC
    TGGCAGATGC CCAACGGGCA CTATCGGCGG CGGAGGCGCG GGGGGCCTGG GCCAGGCATG
    ATGTGTGGGG CTGTCAACAA CCTGCTAAGT GACACGGAAG AAGATGACAA ATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90475
    Clone ID Related Accession (Same CDS sequence) XM_017002245.1
    Accession Version XM_017002245.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC
    ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG
    TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG
    GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC
    TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC
    CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC
    CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC
    CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT
    CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG
    CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA
    GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA
    GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA
    GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG
    GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG
    GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG
    GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC
    ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC
    GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA
    GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG
    CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC
    TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT
    GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC
    GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC
    GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG
    TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG
    GCCTTTGCTC TGGCAACCAA CAAAGGACGG GACATCAAGA CCATCAAGTC TCTGCGGGTG
    CTCCGAGTTC TAAGGCCACT GAAAACCATC AAGCGCTTGC CCAAGCTCAA GGCCGTCTTC
    GACTGCGTAG TGACCTCCTT GAAGAATGTC TTCAACATAC TCATTGTGTA CAAGCTCTTC
    ATGTTCATCT TTGCTGTCAT CGCAGTTCAG CTCTTCAAGG GAAAGTTCTT TTATTGCACG
    GACAGTTCCA AGGACACAGA GAAGGAGTGC ATAGGCAACT ATGTAGATCA TGAGAAAAAC
    AAGATGGAGG TGAAGGGCCG GGAATGGAAG CGCCATGAAT TCCACTACGA CAACATTATC
    TGGGCCCTGC TGACCCTCTT CACCGTCTCC ACAGGGGAAG GATGGCCTCA AGTTCTGCAG
    CACTCTGTAG ATGTGACAGA GGAAGACCGA GGCCCAAGCC GCAGCAACCG CATGGAGATG
    TCTATCTTTT ATGTAGTCTA CTTTGTGGTC TTCCCCTTCT TCTTTGTCAA TATCTTTGTG
    GCTCTCATCA TCATCACCTT CCAGGAGCAA GGGGATAAGA TGATGGAGGA GTGCAGCCTG
    GAGAAGAATG AGAGGGCGTG CATCGACTTC GCCATCAGCG CCAAACCTCT CACCCGCTAC
    ATGCCGCAGA ACAGACACAC CTTCCAGTAC CGCGTGTGGC ACTTTGTGGT GTCTCCGTCC
    TTTGAGTACA CCATTATGGC CATGATCGCC TTGAATACTG TTGTGCTGAT GATGAAGTAT
    TATTCTGCTC CCTGTACCTA TGAGCTGGCC CTGAAGTACC TGAATATCGC CTTCACCATG
    GTGTTTTCCC TGGAATGTGT CCTGAAGGTC ATCGCTTTTG GCTTTTTGAA CTATTTCCGA
    GACACCTGGA ATATCTTTGA CTTCATCACC GTGATTGGCA GTATCACAGA AATTATCCTG
    ACAGACAGCA AGCTGGTGAA CACCAGTGGC TTCAATATGA GCTTTCTGAA GCTCTTCCGA
    GCTGCCCGCC TCATAAAGCT CCTGCGTCAG GGCTATACCA TACGCATTTT GCTGTGGACC
    TTTGTGCAGT CCTTTAAGGC CCTCCCTTAT GTCTGCCTTT TAATTGCCAT GCTTTTCTTC
    ATTTATGCCA TCATTGGGAT GCAGGTATTT GGAAACATAA AATTAGACGA GGAGAGTCAC
    ATCAACCGGC ACAACAACTT CCGGAGTTTC TTTGGGTCCC TAATGCTACT CTTCAGGAGT
    GCCACAGGTG AGGCCTGGCA GGAGATTATG CTGTCATGCC TTGGGGAGAA GGGCTGTGAG
    CCTGACACCA CCGCACCATC AGGGCAGAAC GAGAACGAAC GCTGCGGCAC CGATCTGGCC
    TACGTGTACT TTGTCTCCTT CATCTTCTTC TGCTCCTTCT TGATGCTCAA CCTGTTTGTG
    GCCGTCATCA TGGACAACTT TGAGTACCTG ACTCGGGACT CCTCCATCCT GGGGCCTCAC
    CACTTGGACG AGTTTGTCCG CGTCTGGGCA GAATATGACC GAGCAGCATG TGGCCGCATC
    CATTACACTG AGATGTATGA AATGCTGACT CTCATGTCAC CTCCGCTAGG CCTCGGCAAG
    AGATGTCCCT CCAAAGTGGC ATATAAGAGG TTGGTCCTGA TGAACATGCC AGTAGCTGAG
    GACATGACGG TCCACTTCAC CTCCACACTT ATGGCTCTGA TCCGGACAGC TCTGGACATT
    AAAATTGCCA AAGGTGGTGC AGACAGGCAG CAGCTAGACT CAGAGCTACA AAAGGAGACC
    CTAGCCATCT GGCCTCACCT ATCCCAGAAG ATGCTGGATC TGCTTGTGCC CATGCCCAAA
    GCCTCTGACC TGACTGTGGG CAAAATCTAT GCAGCAATGA TGATCATGGA CTACTATAAG
    CAGAGTAAGG TGAAGAAGCA GAGGCAGCAG CTGGAGGAAC AGAAAAATGC CCCCATGTTC
    CAGCGCATGG AGCCTTCATC TCTGCCTCAG GAGATCATTG CTAATGCCAA AGCCCTGCCT
    TACCTCCAGC AGGACCCCGT TTCAGGCCTG AGTGGCCGGA GTGGATACCC TTCGATGAGT
    CCACTCTCTC CCCAGGATAT ATTCCAGTTG GCTTGTATGG ACCCCGCCGA TGACGGACAG
    TTCCAAGAAC GGCAGTCTCT GGAGCCAGAG GTTAGTGAAT TAAAAAGCGT GCAGCCCTCT
    AACCATGGCA TCTACCTTCC TTCGGACACC CAGGAGCATG CGGGATCTGG GAGGGCATCT
    TCTATGCCAC GTCTGACTGT GGATCCCCAG GTGGTGACAG ACCCTAGCTC CATGAGACGT
    TCATTTTCCA CTATTCGGGA TAAGCGTTCA AATTCCTCGT GGTTGGAGGA ATTCTCCATG
    GAGCGAAGCA GTGAAAATAC CTACAAGTCC CGTCGCCGGA GTTACCACTC CTCCTTGCGG
    CTGTCAGCCC ACCGCCTGAA CTCTGATTCA GGCCACAAGT CTGACACTCA CCGCTCAGGG
    GGCAGGGAGC GGGGACGATC AAAAGAGCGA AAGCATCTTC TCTCTCCTGA TGTCTCCCGC
    TGCAATTCAG AAGAGCGAGG GACCCAGGCT GACTGGGAGT CCCCAGAGCG CCGTCAATCC
    AGGTCACCCA GTGAGGGCAG GTCACAGACG CCCAACAGAC AGGGCACAGG TTCCCTAAGT
    GAGAGCTCCA TCCCCTCTGT CTCTGACACC AGCACCCCAA GAAGAAGTCG TCGGCAGCTC
    CCACCCGTCC CGCCAAAGCC CCGGCCCCTC CTTTCCTACA GCTCCCTGAT TCGACACGCG
    GGCAGCATCT CTCCACCTGC TGATGGAAGC GAGGAGGGCT CCCCGCTGAC CTCCCAAGCT
    CTGGAGAGCA ACAATGCTTG CCTGACCGAG TCTTCCAACT CTCCGCACCC CCAGCAGAGC
    CAACATGCCT CCCCACAGCG CTACATCTCC GAGCCCTACT TGGCCCTGCA CGAAGACTCC
    CACGCCTCAG ACTGTGGTGA GGAGGAGACG CTCACTTTCG AAGCAGCCGT GGCTACTAGC
    CTGGGCCGTT CCAACACCAT CGGCTCAGCC CCACCCCTGC GGCATAGCTG GCAGATGCCC
    AACGGGCACT ATCGGCGGCG GAGGCGCGGG GGGCCTGGGC CAGGCATGAT GTGTGGGGCT
    GTCAACAACC TGCTAAGTGA CACGGAAGAA GATGACAAAT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016857734.1
    CDS427..7791
    Translation

    Target ORF information:

    RefSeq Version XM_017002245.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002245.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC
    ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG
    TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG
    GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC
    TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC
    CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC
    CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC
    CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT
    CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG
    CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA
    GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA
    GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA
    GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG
    GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG
    GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG
    GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC
    ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC
    GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA
    GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG
    CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC
    TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT
    GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC
    GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC
    GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG
    TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG
    GCCTTTGCTC TGGCAACCAA CAAAGGACGG GACATCAAGA CCATCAAGTC TCTGCGGGTG
    CTCCGAGTTC TAAGGCCACT GAAAACCATC AAGCGCTTGC CCAAGCTCAA GGCCGTCTTC
    GACTGCGTAG TGACCTCCTT GAAGAATGTC TTCAACATAC TCATTGTGTA CAAGCTCTTC
    ATGTTCATCT TTGCTGTCAT CGCAGTTCAG CTCTTCAAGG GAAAGTTCTT TTATTGCACG
    GACAGTTCCA AGGACACAGA GAAGGAGTGC ATAGGCAACT ATGTAGATCA TGAGAAAAAC
    AAGATGGAGG TGAAGGGCCG GGAATGGAAG CGCCATGAAT TCCACTACGA CAACATTATC
    TGGGCCCTGC TGACCCTCTT CACCGTCTCC ACAGGGGAAG GATGGCCTCA AGTTCTGCAG
    CACTCTGTAG ATGTGACAGA GGAAGACCGA GGCCCAAGCC GCAGCAACCG CATGGAGATG
    TCTATCTTTT ATGTAGTCTA CTTTGTGGTC TTCCCCTTCT TCTTTGTCAA TATCTTTGTG
    GCTCTCATCA TCATCACCTT CCAGGAGCAA GGGGATAAGA TGATGGAGGA GTGCAGCCTG
    GAGAAGAATG AGAGGGCGTG CATCGACTTC GCCATCAGCG CCAAACCTCT CACCCGCTAC
    ATGCCGCAGA ACAGACACAC CTTCCAGTAC CGCGTGTGGC ACTTTGTGGT GTCTCCGTCC
    TTTGAGTACA CCATTATGGC CATGATCGCC TTGAATACTG TTGTGCTGAT GATGAAGTAT
    TATTCTGCTC CCTGTACCTA TGAGCTGGCC CTGAAGTACC TGAATATCGC CTTCACCATG
    GTGTTTTCCC TGGAATGTGT CCTGAAGGTC ATCGCTTTTG GCTTTTTGAA CTATTTCCGA
    GACACCTGGA ATATCTTTGA CTTCATCACC GTGATTGGCA GTATCACAGA AATTATCCTG
    ACAGACAGCA AGCTGGTGAA CACCAGTGGC TTCAATATGA GCTTTCTGAA GCTCTTCCGA
    GCTGCCCGCC TCATAAAGCT CCTGCGTCAG GGCTATACCA TACGCATTTT GCTGTGGACC
    TTTGTGCAGT CCTTTAAGGC CCTCCCTTAT GTCTGCCTTT TAATTGCCAT GCTTTTCTTC
    ATTTATGCCA TCATTGGGAT GCAGGTATTT GGAAACATAA AATTAGACGA GGAGAGTCAC
    ATCAACCGGC ACAACAACTT CCGGAGTTTC TTTGGGTCCC TAATGCTACT CTTCAGGAGT
    GCCACAGGTG AGGCCTGGCA GGAGATTATG CTGTCATGCC TTGGGGAGAA GGGCTGTGAG
    CCTGACACCA CCGCACCATC AGGGCAGAAC GAGAACGAAC GCTGCGGCAC CGATCTGGCC
    TACGTGTACT TTGTCTCCTT CATCTTCTTC TGCTCCTTCT TGATGCTCAA CCTGTTTGTG
    GCCGTCATCA TGGACAACTT TGAGTACCTG ACTCGGGACT CCTCCATCCT GGGGCCTCAC
    CACTTGGACG AGTTTGTCCG CGTCTGGGCA GAATATGACC GAGCAGCATG TGGCCGCATC
    CATTACACTG AGATGTATGA AATGCTGACT CTCATGTCAC CTCCGCTAGG CCTCGGCAAG
    AGATGTCCCT CCAAAGTGGC ATATAAGAGG TTGGTCCTGA TGAACATGCC AGTAGCTGAG
    GACATGACGG TCCACTTCAC CTCCACACTT ATGGCTCTGA TCCGGACAGC TCTGGACATT
    AAAATTGCCA AAGGTGGTGC AGACAGGCAG CAGCTAGACT CAGAGCTACA AAAGGAGACC
    CTAGCCATCT GGCCTCACCT ATCCCAGAAG ATGCTGGATC TGCTTGTGCC CATGCCCAAA
    GCCTCTGACC TGACTGTGGG CAAAATCTAT GCAGCAATGA TGATCATGGA CTACTATAAG
    CAGAGTAAGG TGAAGAAGCA GAGGCAGCAG CTGGAGGAAC AGAAAAATGC CCCCATGTTC
    CAGCGCATGG AGCCTTCATC TCTGCCTCAG GAGATCATTG CTAATGCCAA AGCCCTGCCT
    TACCTCCAGC AGGACCCCGT TTCAGGCCTG AGTGGCCGGA GTGGATACCC TTCGATGAGT
    CCACTCTCTC CCCAGGATAT ATTCCAGTTG GCTTGTATGG ACCCCGCCGA TGACGGACAG
    TTCCAAGAAC GGCAGTCTCT GGAGCCAGAG GTTAGTGAAT TAAAAAGCGT GCAGCCCTCT
    AACCATGGCA TCTACCTTCC TTCGGACACC CAGGAGCATG CGGGATCTGG GAGGGCATCT
    TCTATGCCAC GTCTGACTGT GGATCCCCAG GTGGTGACAG ACCCTAGCTC CATGAGACGT
    TCATTTTCCA CTATTCGGGA TAAGCGTTCA AATTCCTCGT GGTTGGAGGA ATTCTCCATG
    GAGCGAAGCA GTGAAAATAC CTACAAGTCC CGTCGCCGGA GTTACCACTC CTCCTTGCGG
    CTGTCAGCCC ACCGCCTGAA CTCTGATTCA GGCCACAAGT CTGACACTCA CCGCTCAGGG
    GGCAGGGAGC GGGGACGATC AAAAGAGCGA AAGCATCTTC TCTCTCCTGA TGTCTCCCGC
    TGCAATTCAG AAGAGCGAGG GACCCAGGCT GACTGGGAGT CCCCAGAGCG CCGTCAATCC
    AGGTCACCCA GTGAGGGCAG GTCACAGACG CCCAACAGAC AGGGCACAGG TTCCCTAAGT
    GAGAGCTCCA TCCCCTCTGT CTCTGACACC AGCACCCCAA GAAGAAGTCG TCGGCAGCTC
    CCACCCGTCC CGCCAAAGCC CCGGCCCCTC CTTTCCTACA GCTCCCTGAT TCGACACGCG
    GGCAGCATCT CTCCACCTGC TGATGGAAGC GAGGAGGGCT CCCCGCTGAC CTCCCAAGCT
    CTGGAGAGCA ACAATGCTTG CCTGACCGAG TCTTCCAACT CTCCGCACCC CCAGCAGAGC
    CAACATGCCT CCCCACAGCG CTACATCTCC GAGCCCTACT TGGCCCTGCA CGAAGACTCC
    CACGCCTCAG ACTGTGGTGA GGAGGAGACG CTCACTTTCG AAGCAGCCGT GGCTACTAGC
    CTGGGCCGTT CCAACACCAT CGGCTCAGCC CCACCCCTGC GGCATAGCTG GCAGATGCCC
    AACGGGCACT ATCGGCGGCG GAGGCGCGGG GGGCCTGGGC CAGGCATGAT GTGTGGGGCT
    GTCAACAACC TGCTAAGTGA CACGGAAGAA GATGACAAAT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90476
    Clone ID Related Accession (Same CDS sequence) XM_017002246.1
    Accession Version XM_017002246.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7365bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC
    ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG
    TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG
    GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC
    TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC
    CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC
    CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC
    CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT
    CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG
    CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA
    GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA
    GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA
    GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG
    GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG
    GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG
    GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC
    ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC
    GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA
    GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG
    CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC
    TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT
    GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC
    GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC
    GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG
    TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG
    GCCTTTGCTC TGGCAACCAA CAAAGGACGG GACATCAAGA CCATCAAGTC TCTGCGGGTG
    CTCCGAGTTC TAAGGCCACT GAAAACCATC AAGCGCTTGC CCAAGCTCAA GGCCGTCTTC
    GACTGCGTAG TGACCTCCTT GAAGAATGTC TTCAACATAC TCATTGTGTA CAAGCTCTTC
    ATGTTCATCT TTGCTGTCAT CGCAGTTCAG CTCTTCAAGG GAAAGTTCTT TTATTGCACG
    GACAGTTCCA AGGACACAGA GAAGGAGTGC ATAGGCAACT ATGTAGATCA TGAGAAAAAC
    AAGATGGAGG TGAAGGGCCG GGAATGGAAG CGCCATGAAT TCCACTACGA CAACATTATC
    TGGGCCCTGC TGACCCTCTT CACCGTCTCC ACAGGGGAAG GATGGCCTCA AGTTCTGCAG
    CACTCTGTAG ATGTGACAGA GGAAGACCGA GGCCCAAGCC GCAGCAACCG CATGGAGATG
    TCTATCTTTT ATGTAGTCTA CTTTGTGGTC TTCCCCTTCT TCTTTGTCAA TATCTTTGTG
    GCTCTCATCA TCATCACCTT CCAGGAGCAA GGGGATAAGA TGATGGAGGA GTGCAGCCTG
    GAGAAGAATG AGAGGGCGTG CATCGACTTC GCCATCAGCG CCAAACCTCT CACCCGCTAC
    ATGCCGCAGA ACAGACACAC CTTCCAGTAC CGCGTGTGGC ACTTTGTGGT GTCTCCGTCC
    TTTGAGTACA CCATTATGGC CATGATCGCC TTGAATACTG TTGTGCTGAT GATGAAGTAT
    TATTCTGCTC CCTGTACCTA TGAGCTGGCC CTGAAGTACC TGAATATCGC CTTCACCATG
    GTGTTTTCCC TGGAATGTGT CCTGAAGGTC ATCGCTTTTG GCTTTTTGAA CTATTTCCGA
    GACACCTGGA ATATCTTTGA CTTCATCACC GTGATTGGCA GTATCACAGA AATTATCCTG
    ACAGACAGCA AGCTGGTGAA CACCAGTGGC TTCAATATGA GCTTTCTGAA GCTCTTCCGA
    GCTGCCCGCC TCATAAAGCT CCTGCGTCAG GGCTATACCA TACGCATTTT GCTGTGGACC
    TTTGTGCAGT CCTTTAAGGC CCTCCCTTAT GTCTGCCTTT TAATTGCCAT GCTTTTCTTC
    ATTTATGCCA TCATTGGGAT GCAGGTATTT GGAAACATAA AATTAGACGA GGAGAGTCAC
    ATCAACCGGC ACAACAACTT CCGGAGTTTC TTTGGGTCCC TAATGCTACT CTTCAGGAGT
    GCCACAGGTG AGGCCTGGCA GGAGATTATG CTGTCATGCC TTGGGGAGAA GGGCTGTGAG
    CCTGACACCA CCGCACCATC AGGGCAGAAC GAGAACGAAC GCTGCGGCAC CGATCTGGCC
    TACGTGTACT TTGTCTCCTT CATCTTCTTC TGCTCCTTCT TGATGCTCAA CCTGTTTGTG
    GCCGTCATCA TGGACAACTT TGAGTACCTG ACTCGGGACT CCTCCATCCT GGGGCCTCAC
    CACTTGGACG AGTTTGTCCG CGTCTGGGCA GAATATGACC GAGCAGCATG CGGCAGGATC
    CCGTATAAGG ATATGTACAA ATTAGTGCGG GTGATCTCGC CTCCTCTGGG CCTGGGAGAG
    AACTGCCCCT ACAGGGTGGC TTGCAAGAGG TTGGTCCTGA TGAACATGCC AGTAGCTGAG
    GACATGACGG TCCACTTCAC CTCCACACTT ATGGCTCTGA TCCGGACAGC TCTGGACATT
    AAAATTGCCA AAGGTGGTGC AGACAGGCAG CAGCTAGACT CAGAGCTACA AAAGGAGACC
    CTAGCCATCT GGCCTCACCT ATCCCAGAAG ATGCTGGATC TGCTTGTGCC CATGCCCAAA
    GCCTCTGACC TGACTGTGGG CAAAATCTAT GCAGCAATGA TGATCATGGA CTACTATAAG
    CAGAGTAAGG TGAAGAAGCA GAGGCAGCAG CTGGAGGAAC AGAAAAATGC CCCCATGTTC
    CAGCGCATGG AGCCTTCATC TCTGCCTCAG GAGATCATTG CTAATGCCAA AGCCCTGCCT
    TACCTCCAGC AGGACCCCGT TTCAGGCCTG AGTGGCCGGA GTGGATACCC TTCGATGAGT
    CCACTCTCTC CCCAGGATAT ATTCCAGTTG GCTTGTATGG ACCCCGCCGA TGACGGACAG
    TTCCAAGAAC GGCAGTCTCT GGAGCCAGAG GTTAGTGAAT TAAAAAGCGT GCAGCCCTCT
    AACCATGGCA TCTACCTTCC TTCGGACACC CAGGAGCATG CGGGATCTGG GAGGGCATCT
    TCTATGCCAC GTCTGACTGT GGATCCCCAG GTGGTGACAG ACCCTAGCTC CATGAGACGT
    TCATTTTCCA CTATTCGGGA TAAGCGTTCA AATTCCTCGT GGTTGGAGGA ATTCTCCATG
    GAGCGAAGCA GTGAAAATAC CTACAAGTCC CGTCGCCGGA GTTACCACTC CTCCTTGCGG
    CTGTCAGCCC ACCGCCTGAA CTCTGATTCA GGCCACAAGT CTGACACTCA CCGCTCAGGG
    GGCAGGGAGC GGGGACGATC AAAAGAGCGA AAGCATCTTC TCTCTCCTGA TGTCTCCCGC
    TGCAATTCAG AAGAGCGAGG GACCCAGGCT GACTGGGAGT CCCCAGAGCG CCGTCAATCC
    AGGTCACCCA GTGAGGGCAG GTCACAGACG CCCAACAGAC AGGGCACAGG TTCCCTAAGT
    GAGAGCTCCA TCCCCTCTGT CTCTGACACC AGCACCCCAA GAAGAAGTCG TCGGCAGCTC
    CCACCCGTCC CGCCAAAGCC CCGGCCCCTC CTTTCCTACA GCTCCCTGAT TCGACACGCG
    GGCAGCATCT CTCCACCTGC TGATGGAAGC GAGGAGGGCT CCCCGCTGAC CTCCCAAGCT
    CTGGAGAGCA ACAATGCTTG CCTGACCGAG TCTTCCAACT CTCCGCACCC CCAGCAGAGC
    CAACATGCCT CCCCACAGCG CTACATCTCC GAGCCCTACT TGGCCCTGCA CGAAGACTCC
    CACGCCTCAG ACTGTGGTGA GGAGGAGACG CTCACTTTCG AAGCAGCCGT GGCTACTAGC
    CTGGGCCGTT CCAACACCAT CGGCTCAGCC CCACCCCTGC GGCATAGCTG GCAGATGCCC
    AACGGGCACT ATCGGCGGCG GAGGCGCGGG GGGCCTGGGC CAGGCATGAT GTGTGGGGCT
    GTCAACAACC TGCTAAGTGA CACGGAAGAA GATGACAAAT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016857735.1
    CDS427..7791
    Translation

    Target ORF information:

    RefSeq Version XM_017002246.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002246.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC
    ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG
    TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG
    GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC
    TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC
    CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC
    CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC
    CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT
    CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG
    CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA
    GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA
    GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA
    GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG
    GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG
    GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG
    GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC
    ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC
    GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA
    GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG
    CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC
    TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT
    GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC
    GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC
    GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG
    TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG
    GCCTTTGCTC TGGCAACCAA CAAAGGACGG GACATCAAGA CCATCAAGTC TCTGCGGGTG
    CTCCGAGTTC TAAGGCCACT GAAAACCATC AAGCGCTTGC CCAAGCTCAA GGCCGTCTTC
    GACTGCGTAG TGACCTCCTT GAAGAATGTC TTCAACATAC TCATTGTGTA CAAGCTCTTC
    ATGTTCATCT TTGCTGTCAT CGCAGTTCAG CTCTTCAAGG GAAAGTTCTT TTATTGCACG
    GACAGTTCCA AGGACACAGA GAAGGAGTGC ATAGGCAACT ATGTAGATCA TGAGAAAAAC
    AAGATGGAGG TGAAGGGCCG GGAATGGAAG CGCCATGAAT TCCACTACGA CAACATTATC
    TGGGCCCTGC TGACCCTCTT CACCGTCTCC ACAGGGGAAG GATGGCCTCA AGTTCTGCAG
    CACTCTGTAG ATGTGACAGA GGAAGACCGA GGCCCAAGCC GCAGCAACCG CATGGAGATG
    TCTATCTTTT ATGTAGTCTA CTTTGTGGTC TTCCCCTTCT TCTTTGTCAA TATCTTTGTG
    GCTCTCATCA TCATCACCTT CCAGGAGCAA GGGGATAAGA TGATGGAGGA GTGCAGCCTG
    GAGAAGAATG AGAGGGCGTG CATCGACTTC GCCATCAGCG CCAAACCTCT CACCCGCTAC
    ATGCCGCAGA ACAGACACAC CTTCCAGTAC CGCGTGTGGC ACTTTGTGGT GTCTCCGTCC
    TTTGAGTACA CCATTATGGC CATGATCGCC TTGAATACTG TTGTGCTGAT GATGAAGTAT
    TATTCTGCTC CCTGTACCTA TGAGCTGGCC CTGAAGTACC TGAATATCGC CTTCACCATG
    GTGTTTTCCC TGGAATGTGT CCTGAAGGTC ATCGCTTTTG GCTTTTTGAA CTATTTCCGA
    GACACCTGGA ATATCTTTGA CTTCATCACC GTGATTGGCA GTATCACAGA AATTATCCTG
    ACAGACAGCA AGCTGGTGAA CACCAGTGGC TTCAATATGA GCTTTCTGAA GCTCTTCCGA
    GCTGCCCGCC TCATAAAGCT CCTGCGTCAG GGCTATACCA TACGCATTTT GCTGTGGACC
    TTTGTGCAGT CCTTTAAGGC CCTCCCTTAT GTCTGCCTTT TAATTGCCAT GCTTTTCTTC
    ATTTATGCCA TCATTGGGAT GCAGGTATTT GGAAACATAA AATTAGACGA GGAGAGTCAC
    ATCAACCGGC ACAACAACTT CCGGAGTTTC TTTGGGTCCC TAATGCTACT CTTCAGGAGT
    GCCACAGGTG AGGCCTGGCA GGAGATTATG CTGTCATGCC TTGGGGAGAA GGGCTGTGAG
    CCTGACACCA CCGCACCATC AGGGCAGAAC GAGAACGAAC GCTGCGGCAC CGATCTGGCC
    TACGTGTACT TTGTCTCCTT CATCTTCTTC TGCTCCTTCT TGATGCTCAA CCTGTTTGTG
    GCCGTCATCA TGGACAACTT TGAGTACCTG ACTCGGGACT CCTCCATCCT GGGGCCTCAC
    CACTTGGACG AGTTTGTCCG CGTCTGGGCA GAATATGACC GAGCAGCATG CGGCAGGATC
    CCGTATAAGG ATATGTACAA ATTAGTGCGG GTGATCTCGC CTCCTCTGGG CCTGGGAGAG
    AACTGCCCCT ACAGGGTGGC TTGCAAGAGG TTGGTCCTGA TGAACATGCC AGTAGCTGAG
    GACATGACGG TCCACTTCAC CTCCACACTT ATGGCTCTGA TCCGGACAGC TCTGGACATT
    AAAATTGCCA AAGGTGGTGC AGACAGGCAG CAGCTAGACT CAGAGCTACA AAAGGAGACC
    CTAGCCATCT GGCCTCACCT ATCCCAGAAG ATGCTGGATC TGCTTGTGCC CATGCCCAAA
    GCCTCTGACC TGACTGTGGG CAAAATCTAT GCAGCAATGA TGATCATGGA CTACTATAAG
    CAGAGTAAGG TGAAGAAGCA GAGGCAGCAG CTGGAGGAAC AGAAAAATGC CCCCATGTTC
    CAGCGCATGG AGCCTTCATC TCTGCCTCAG GAGATCATTG CTAATGCCAA AGCCCTGCCT
    TACCTCCAGC AGGACCCCGT TTCAGGCCTG AGTGGCCGGA GTGGATACCC TTCGATGAGT
    CCACTCTCTC CCCAGGATAT ATTCCAGTTG GCTTGTATGG ACCCCGCCGA TGACGGACAG
    TTCCAAGAAC GGCAGTCTCT GGAGCCAGAG GTTAGTGAAT TAAAAAGCGT GCAGCCCTCT
    AACCATGGCA TCTACCTTCC TTCGGACACC CAGGAGCATG CGGGATCTGG GAGGGCATCT
    TCTATGCCAC GTCTGACTGT GGATCCCCAG GTGGTGACAG ACCCTAGCTC CATGAGACGT
    TCATTTTCCA CTATTCGGGA TAAGCGTTCA AATTCCTCGT GGTTGGAGGA ATTCTCCATG
    GAGCGAAGCA GTGAAAATAC CTACAAGTCC CGTCGCCGGA GTTACCACTC CTCCTTGCGG
    CTGTCAGCCC ACCGCCTGAA CTCTGATTCA GGCCACAAGT CTGACACTCA CCGCTCAGGG
    GGCAGGGAGC GGGGACGATC AAAAGAGCGA AAGCATCTTC TCTCTCCTGA TGTCTCCCGC
    TGCAATTCAG AAGAGCGAGG GACCCAGGCT GACTGGGAGT CCCCAGAGCG CCGTCAATCC
    AGGTCACCCA GTGAGGGCAG GTCACAGACG CCCAACAGAC AGGGCACAGG TTCCCTAAGT
    GAGAGCTCCA TCCCCTCTGT CTCTGACACC AGCACCCCAA GAAGAAGTCG TCGGCAGCTC
    CCACCCGTCC CGCCAAAGCC CCGGCCCCTC CTTTCCTACA GCTCCCTGAT TCGACACGCG
    GGCAGCATCT CTCCACCTGC TGATGGAAGC GAGGAGGGCT CCCCGCTGAC CTCCCAAGCT
    CTGGAGAGCA ACAATGCTTG CCTGACCGAG TCTTCCAACT CTCCGCACCC CCAGCAGAGC
    CAACATGCCT CCCCACAGCG CTACATCTCC GAGCCCTACT TGGCCCTGCA CGAAGACTCC
    CACGCCTCAG ACTGTGGTGA GGAGGAGACG CTCACTTTCG AAGCAGCCGT GGCTACTAGC
    CTGGGCCGTT CCAACACCAT CGGCTCAGCC CCACCCCTGC GGCATAGCTG GCAGATGCCC
    AACGGGCACT ATCGGCGGCG GAGGCGCGGG GGGCCTGGGC CAGGCATGAT GTGTGGGGCT
    GTCAACAACC TGCTAAGTGA CACGGAAGAA GATGACAAAT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90477
    Clone ID Related Accession (Same CDS sequence) XM_017002247.1
    Accession Version XM_017002247.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7320bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAGAGGG AGCGGAGGCG CCGGCACCAC
    ATGTCCGTGT GGGAGCAGCG TACCAGCCAG CTGAGGAAGC ACATGCAGAT GTCCAGCCAG
    GAGGCCCTCA ACAGAGAGGA GGCGCCGACC ATGAACCCGC TCAACCCCCT CAACCCGCTC
    AGCTCCCTCA ACCCGCTCAA TGCCCACCCC AGCCTTTATC GGCGACCCAG GGCCATTGAG
    GGCCTGGCCC TGGGCCTGGC CCTGGAGAAG TTCGAGGAGG AGCGCATCAG CCGTGGGGGG
    TCCCTCAAGG GGGATGGAGG GGACCGATCC AGTGCCCTGG ACAACCAGAG GACCCCTTTG
    TCCCTGGGCC AGCGGGAGCC ACCATGGCTG GCCAGGCCCT GTCATGGAAA CTGTGACCCG
    ACTCAGCAGG AGGCAGGGGG AGGAGAGGCT GTGGTGACCT TTGAGGACCG GGCCAGGCAC
    AGGCAGAGCC AACGGCGCAG CCGGCATCGC CGCGTCAGGA CAGAAGGCAA GGAGTCCTCT
    TCAGCCTCCC GGAGCAGGTC TGCCAGCCAG GAACGCAGTC TGGATGAAGC CATGCCCACT
    GAAGGGGAGA AGGACCATGA GCTCAGGGGC AACCATGGTG CCAAGGAGCC AACGATCCAA
    GAAGAGAGAG CCCAGGATTT AAGGAGGACC AACAGTCTGA TGGTGTCCAG AGGCTCCGGG
    CTGGCAGGAG GCCTTGATGA GGCTGACACC CCCCTAGTCC TGCCCCATCC TGAGCTGGAA
    GTGGGGAAGC ACGTGGTGCT GACGGAGCAG GAGCCAGAAG GCAGCAGTGA GCAGGCCCTG
    CTGGGGAATG TGCAGCTAGA CATGGGCCGG GTCATCAGCC AGAGCGAGCC TGACCTCTCC
    TGCATCACGG CCAACACGGA CAAGGCCACC ACCGAGAGCA CCAGCGTCAC CGTCGCCATC
    CCCGACGTGG ACCCCTTGGT GGACTCAACC GTGGTGCACA TTAGCAACAA GACGGATGGG
    GAAGCCAGTC CCTTGAAGGA GGCAGAGATC AGAGAGGATG AGGAGGAGGT GGAGAAGAAG
    AAGCAGAAGA AGGAGAAGCG TGAGACAGGC AAAGCCATGG TGCCCCACAG CTCAATGTTC
    ATCTTCAGCA CCACCAACCC GATCCGGAGG GCCTGCCACT ACATCGTGAA CCTGCGCTAC
    TTTGAGATGT GCATCCTCCT GGTGATTGCA GCCAGCAGCA TCGCCCTGGC GGCAGAGGAC
    CCCGTCCTGA CCAACTCGGA GCGCAACAAA GTCCTGAGGT ATTTTGACTA TGTGTTCACG
    GGCGTGTTCA CCTTTGAGAT GGTTATAAAG ATGATAGACC AAGGCTTGAT CCTGCAGGAT
    GGGTCCTACT TCCGAGACTT GTGGAACATC CTGGACTTTG TGGTGGTCGT TGGCGCATTG
    GTGGCCTTTG CTCTGGCGAA CGCTTTGGGA ACCAACAAAG GACGGGACAT CAAGACCATC
    AAGTCTCTGC GGGTGCTCCG AGTTCTAAGG CCACTGAAAA CCATCAAGCG CTTGCCCAAG
    CTCAAGGCCG TCTTCGACTG CGTAGTGACC TCCTTGAAGA ATGTCTTCAA CATACTCATT
    GTGTACAAGC TCTTCATGTT CATCTTTGCT GTCATCGCAG TTCAGCTCTT CAAGGGAAAG
    TTCTTTTATT GCACGGACAG TTCCAAGGAC ACAGAGAAGG AGTGCATAGG CAACTATGTA
    GATCATGAGA AAAACAAGAT GGAGGTGAAG GGCCGGGAAT GGAAGCGCCA TGAATTCCAC
    TACGACAACA TTATCTGGGC CCTGCTGACC CTCTTCACCG TCTCCACAGG GGAAGGATGG
    CCTCAAGTTC TGCAGCACTC TGTAGATGTG ACAGAGGAAG ACCGAGGCCC AAGCCGCAGC
    AACCGCATGG AGATGTCTAT CTTTTATGTA GTCTACTTTG TGGTCTTCCC CTTCTTCTTT
    GTCAATATCT TTGTGGCTCT CATCATCATC ACCTTCCAGG AGCAAGGGGA TAAGATGATG
    GAGGAGTGCA GCCTGGAGAA GAATGAGAGG GCGTGCATCG ACTTCGCCAT CAGCGCCAAA
    CCTCTCACCC GCTACATGCC GCAGAACAGA CACACCTTCC AGTACCGCGT GTGGCACTTT
    GTGGTGTCTC CGTCCTTTGA GTACACCATT ATGGCCATGA TCGCCTTGAA TACTGTTGTG
    CTGATGATGA AGTATTATTC TGCTCCCTGT ACCTATGAGC TGGCCCTGAA GTACCTGAAT
    ATCGCCTTCA CCATGGTGTT TTCCCTGGAA TGTGTCCTGA AGGTCATCGC TTTTGGCTTT
    TTGAACTATT TCCGAGACAC CTGGAATATC TTTGACTTCA TCACCGTGAT TGGCAGTATC
    ACAGAAATTA TCCTGACAGA CAGCAAGCTG GTGAACACCA GTGGCTTCAA TATGAGCTTT
    CTGAAGCTCT TCCGAGCTGC CCGCCTCATA AAGCTCCTGC GTCAGGGCTA TACCATACGC
    ATTTTGCTGT GGACCTTTGT GCAGTCCTTT AAGGCCCTCC CTTATGTCTG CCTTTTAATT
    GCCATGCTTT TCTTCATTTA TGCCATCATT GGGATGCAGG TATTTGGAAA CATAAAATTA
    GACGAGGAGA GTCACATCAA CCGGCACAAC AACTTCCGGA GTTTCTTTGG GTCCCTAATG
    CTACTCTTCA GGAGTGCCAC AGGTGAGGCC TGGCAGGAGA TTATGCTGTC ATGCCTTGGG
    GAGAAGGGCT GTGAGCCTGA CACCACCGCA CCATCAGGGC AGAACGAGAA CGAACGCTGC
    GGCACCGATC TGGCCTACGT GTACTTTGTC TCCTTCATCT TCTTCTGCTC CTTCTTGATG
    CTCAACCTGT TTGTGGCCGT CATCATGGAC AACTTTGAGT ACCTGACTCG GGACTCCTCC
    ATCCTGGGGC CTCACCACTT GGACGAGTTT GTCCGCGTCT GGGCAGAATA TGACCGAGCA
    GCATGTGGCC GCATCCATTA CACTGAGATG TATGAAATGC TGACTCTCAT GTCACCTCCG
    CTAGGCCTCG GCAAGAGATG TCCCTCCAAA GTGGCATATA AGAGGTTGGT CCTGATGAAC
    ATGCCAGTAG CTGAGGACAT GACGGTCCAC TTCACCTCCA CACTTATGGC TCTGATCCGG
    ACAGCTCTGG ACATTAAAAT TGCCAAAGGT GGTGCAGACA GGCAGCAGCT AGACTCAGAG
    CTACAAAAGG AGACCCTAGC CATCTGGCCT CACCTATCCC AGAAGATGCT GGATCTGCTT
    GTGCCCATGC CCAAAGCCTC TGACCTGACT GTGGGCAAAA TCTATGCAGC AATGATGATC
    ATGGACTACT ATAAGCAGAG TAAGGTGAAG AAGCAGAGGC AGCAGCTGGA GGAACAGAAA
    AATGCCCCCA TGTTCCAGCG CATGGAGCCT TCATCTCTGC CTCAGGAGAT CATTGCTAAT
    GCCAAAGCCC TGCCTTACCT CCAGCAGGAC CCCGTTTCAG GCCTGAGTGG CCGGAGTGGA
    TACCCTTCGA TGAGTCCACT CTCTCCCCAG GATATATTCC AGTTGGCTTG TATGGACCCC
    GCCGATGACG GACAGTTCCA AGAACGGCAG TCTCTGGAGC CAGAGGTTAG TGAATTAAAA
    AGCGTGCAGC CCTCTAACCA TGGCATCTAC CTTCCTTCGG ACACCCAGGA GCATGCGGGA
    TCTGGGAGGG CATCTTCTAT GCCACGTCTG ACTGTGGATC CCCAGGTGGT GACAGACCCT
    AGCTCCATGA GACGTTCATT TTCCACTATT CGGGATAAGC GTTCAAATTC CTCGTGGTTG
    GAGGAATTCT CCATGGAGCG AAGCAGTGAA AATACCTACA AGTCCCGTCG CCGGAGTTAC
    CACTCCTCCT TGCGGCTGTC AGCCCACCGC CTGAACTCTG ATTCAGGCCA CAAGTCTGAC
    ACTCACCGCT CAGGGGGCAG GGAGCGGGGA CGATCAAAAG AGCGAAAGCA TCTTCTCTCT
    CCTGATGTCT CCCGCTGCAA TTCAGAAGAG CGAGGGACCC AGGCTGACTG GGAGTCCCCA
    GAGCGCCGTC AATCCAGGTC ACCCAGTGAG GGCAGGTCAC AGACGCCCAA CAGACAGGGC
    ACAGGTTCCC TAAGTGAGAG CTCCATCCCC TCTGTCTCTG ACACCAGCAC CCCAAGAAGA
    AGTCGTCGGC AGCTCCCACC CGTCCCGCCA AAGCCCCGGC CCCTCCTTTC CTACAGCTCC
    CTGATTCGAC ACGCGGGCAG CATCTCTCCA CCTGCTGATG GAAGCGAGGA GGGCTCCCCG
    CTGACCTCCC AAGCTCTGGA GAGCAACAAT GCTTGCCTGA CCGAGTCTTC CAACTCTCCG
    CACCCCCAGC AGAGCCAACA TGCCTCCCCA CAGCGCTACA TCTCCGAGCC CTACTTGGCC
    CTGCACGAAG ACTCCCACGC CTCAGACTGT GGTGAGGAGG AGACGCTCAC TTTCGAAGCA
    GCCGTGGCTA CTAGCCTGGG CCGTTCCAAC ACCATCGGCT CAGCCCCACC CCTGCGGCAT
    AGCTGGCAGA TGCCCAACGG GCACTATCGG CGGCGGAGGC GCGGGGGGCC TGGGCCAGGC
    ATGATGTGTG GGGCTGTCAA CAACCTGCTA AGTGACACGG AAGAAGATGA CAAATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016857736.1
    CDS426..7745
    Translation

    Target ORF information:

    RefSeq Version XM_017002247.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002247.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAGAGGG AGCGGAGGCG CCGGCACCAC
    ATGTCCGTGT GGGAGCAGCG TACCAGCCAG CTGAGGAAGC ACATGCAGAT GTCCAGCCAG
    GAGGCCCTCA ACAGAGAGGA GGCGCCGACC ATGAACCCGC TCAACCCCCT CAACCCGCTC
    AGCTCCCTCA ACCCGCTCAA TGCCCACCCC AGCCTTTATC GGCGACCCAG GGCCATTGAG
    GGCCTGGCCC TGGGCCTGGC CCTGGAGAAG TTCGAGGAGG AGCGCATCAG CCGTGGGGGG
    TCCCTCAAGG GGGATGGAGG GGACCGATCC AGTGCCCTGG ACAACCAGAG GACCCCTTTG
    TCCCTGGGCC AGCGGGAGCC ACCATGGCTG GCCAGGCCCT GTCATGGAAA CTGTGACCCG
    ACTCAGCAGG AGGCAGGGGG AGGAGAGGCT GTGGTGACCT TTGAGGACCG GGCCAGGCAC
    AGGCAGAGCC AACGGCGCAG CCGGCATCGC CGCGTCAGGA CAGAAGGCAA GGAGTCCTCT
    TCAGCCTCCC GGAGCAGGTC TGCCAGCCAG GAACGCAGTC TGGATGAAGC CATGCCCACT
    GAAGGGGAGA AGGACCATGA GCTCAGGGGC AACCATGGTG CCAAGGAGCC AACGATCCAA
    GAAGAGAGAG CCCAGGATTT AAGGAGGACC AACAGTCTGA TGGTGTCCAG AGGCTCCGGG
    CTGGCAGGAG GCCTTGATGA GGCTGACACC CCCCTAGTCC TGCCCCATCC TGAGCTGGAA
    GTGGGGAAGC ACGTGGTGCT GACGGAGCAG GAGCCAGAAG GCAGCAGTGA GCAGGCCCTG
    CTGGGGAATG TGCAGCTAGA CATGGGCCGG GTCATCAGCC AGAGCGAGCC TGACCTCTCC
    TGCATCACGG CCAACACGGA CAAGGCCACC ACCGAGAGCA CCAGCGTCAC CGTCGCCATC
    CCCGACGTGG ACCCCTTGGT GGACTCAACC GTGGTGCACA TTAGCAACAA GACGGATGGG
    GAAGCCAGTC CCTTGAAGGA GGCAGAGATC AGAGAGGATG AGGAGGAGGT GGAGAAGAAG
    AAGCAGAAGA AGGAGAAGCG TGAGACAGGC AAAGCCATGG TGCCCCACAG CTCAATGTTC
    ATCTTCAGCA CCACCAACCC GATCCGGAGG GCCTGCCACT ACATCGTGAA CCTGCGCTAC
    TTTGAGATGT GCATCCTCCT GGTGATTGCA GCCAGCAGCA TCGCCCTGGC GGCAGAGGAC
    CCCGTCCTGA CCAACTCGGA GCGCAACAAA GTCCTGAGGT ATTTTGACTA TGTGTTCACG
    GGCGTGTTCA CCTTTGAGAT GGTTATAAAG ATGATAGACC AAGGCTTGAT CCTGCAGGAT
    GGGTCCTACT TCCGAGACTT GTGGAACATC CTGGACTTTG TGGTGGTCGT TGGCGCATTG
    GTGGCCTTTG CTCTGGCGAA CGCTTTGGGA ACCAACAAAG GACGGGACAT CAAGACCATC
    AAGTCTCTGC GGGTGCTCCG AGTTCTAAGG CCACTGAAAA CCATCAAGCG CTTGCCCAAG
    CTCAAGGCCG TCTTCGACTG CGTAGTGACC TCCTTGAAGA ATGTCTTCAA CATACTCATT
    GTGTACAAGC TCTTCATGTT CATCTTTGCT GTCATCGCAG TTCAGCTCTT CAAGGGAAAG
    TTCTTTTATT GCACGGACAG TTCCAAGGAC ACAGAGAAGG AGTGCATAGG CAACTATGTA
    GATCATGAGA AAAACAAGAT GGAGGTGAAG GGCCGGGAAT GGAAGCGCCA TGAATTCCAC
    TACGACAACA TTATCTGGGC CCTGCTGACC CTCTTCACCG TCTCCACAGG GGAAGGATGG
    CCTCAAGTTC TGCAGCACTC TGTAGATGTG ACAGAGGAAG ACCGAGGCCC AAGCCGCAGC
    AACCGCATGG AGATGTCTAT CTTTTATGTA GTCTACTTTG TGGTCTTCCC CTTCTTCTTT
    GTCAATATCT TTGTGGCTCT CATCATCATC ACCTTCCAGG AGCAAGGGGA TAAGATGATG
    GAGGAGTGCA GCCTGGAGAA GAATGAGAGG GCGTGCATCG ACTTCGCCAT CAGCGCCAAA
    CCTCTCACCC GCTACATGCC GCAGAACAGA CACACCTTCC AGTACCGCGT GTGGCACTTT
    GTGGTGTCTC CGTCCTTTGA GTACACCATT ATGGCCATGA TCGCCTTGAA TACTGTTGTG
    CTGATGATGA AGTATTATTC TGCTCCCTGT ACCTATGAGC TGGCCCTGAA GTACCTGAAT
    ATCGCCTTCA CCATGGTGTT TTCCCTGGAA TGTGTCCTGA AGGTCATCGC TTTTGGCTTT
    TTGAACTATT TCCGAGACAC CTGGAATATC TTTGACTTCA TCACCGTGAT TGGCAGTATC
    ACAGAAATTA TCCTGACAGA CAGCAAGCTG GTGAACACCA GTGGCTTCAA TATGAGCTTT
    CTGAAGCTCT TCCGAGCTGC CCGCCTCATA AAGCTCCTGC GTCAGGGCTA TACCATACGC
    ATTTTGCTGT GGACCTTTGT GCAGTCCTTT AAGGCCCTCC CTTATGTCTG CCTTTTAATT
    GCCATGCTTT TCTTCATTTA TGCCATCATT GGGATGCAGG TATTTGGAAA CATAAAATTA
    GACGAGGAGA GTCACATCAA CCGGCACAAC AACTTCCGGA GTTTCTTTGG GTCCCTAATG
    CTACTCTTCA GGAGTGCCAC AGGTGAGGCC TGGCAGGAGA TTATGCTGTC ATGCCTTGGG
    GAGAAGGGCT GTGAGCCTGA CACCACCGCA CCATCAGGGC AGAACGAGAA CGAACGCTGC
    GGCACCGATC TGGCCTACGT GTACTTTGTC TCCTTCATCT TCTTCTGCTC CTTCTTGATG
    CTCAACCTGT TTGTGGCCGT CATCATGGAC AACTTTGAGT ACCTGACTCG GGACTCCTCC
    ATCCTGGGGC CTCACCACTT GGACGAGTTT GTCCGCGTCT GGGCAGAATA TGACCGAGCA
    GCATGTGGCC GCATCCATTA CACTGAGATG TATGAAATGC TGACTCTCAT GTCACCTCCG
    CTAGGCCTCG GCAAGAGATG TCCCTCCAAA GTGGCATATA AGAGGTTGGT CCTGATGAAC
    ATGCCAGTAG CTGAGGACAT GACGGTCCAC TTCACCTCCA CACTTATGGC TCTGATCCGG
    ACAGCTCTGG ACATTAAAAT TGCCAAAGGT GGTGCAGACA GGCAGCAGCT AGACTCAGAG
    CTACAAAAGG AGACCCTAGC CATCTGGCCT CACCTATCCC AGAAGATGCT GGATCTGCTT
    GTGCCCATGC CCAAAGCCTC TGACCTGACT GTGGGCAAAA TCTATGCAGC AATGATGATC
    ATGGACTACT ATAAGCAGAG TAAGGTGAAG AAGCAGAGGC AGCAGCTGGA GGAACAGAAA
    AATGCCCCCA TGTTCCAGCG CATGGAGCCT TCATCTCTGC CTCAGGAGAT CATTGCTAAT
    GCCAAAGCCC TGCCTTACCT CCAGCAGGAC CCCGTTTCAG GCCTGAGTGG CCGGAGTGGA
    TACCCTTCGA TGAGTCCACT CTCTCCCCAG GATATATTCC AGTTGGCTTG TATGGACCCC
    GCCGATGACG GACAGTTCCA AGAACGGCAG TCTCTGGAGC CAGAGGTTAG TGAATTAAAA
    AGCGTGCAGC CCTCTAACCA TGGCATCTAC CTTCCTTCGG ACACCCAGGA GCATGCGGGA
    TCTGGGAGGG CATCTTCTAT GCCACGTCTG ACTGTGGATC CCCAGGTGGT GACAGACCCT
    AGCTCCATGA GACGTTCATT TTCCACTATT CGGGATAAGC GTTCAAATTC CTCGTGGTTG
    GAGGAATTCT CCATGGAGCG AAGCAGTGAA AATACCTACA AGTCCCGTCG CCGGAGTTAC
    CACTCCTCCT TGCGGCTGTC AGCCCACCGC CTGAACTCTG ATTCAGGCCA CAAGTCTGAC
    ACTCACCGCT CAGGGGGCAG GGAGCGGGGA CGATCAAAAG AGCGAAAGCA TCTTCTCTCT
    CCTGATGTCT CCCGCTGCAA TTCAGAAGAG CGAGGGACCC AGGCTGACTG GGAGTCCCCA
    GAGCGCCGTC AATCCAGGTC ACCCAGTGAG GGCAGGTCAC AGACGCCCAA CAGACAGGGC
    ACAGGTTCCC TAAGTGAGAG CTCCATCCCC TCTGTCTCTG ACACCAGCAC CCCAAGAAGA
    AGTCGTCGGC AGCTCCCACC CGTCCCGCCA AAGCCCCGGC CCCTCCTTTC CTACAGCTCC
    CTGATTCGAC ACGCGGGCAG CATCTCTCCA CCTGCTGATG GAAGCGAGGA GGGCTCCCCG
    CTGACCTCCC AAGCTCTGGA GAGCAACAAT GCTTGCCTGA CCGAGTCTTC CAACTCTCCG
    CACCCCCAGC AGAGCCAACA TGCCTCCCCA CAGCGCTACA TCTCCGAGCC CTACTTGGCC
    CTGCACGAAG ACTCCCACGC CTCAGACTGT GGTGAGGAGG AGACGCTCAC TTTCGAAGCA
    GCCGTGGCTA CTAGCCTGGG CCGTTCCAAC ACCATCGGCT CAGCCCCACC CCTGCGGCAT
    AGCTGGCAGA TGCCCAACGG GCACTATCGG CGGCGGAGGC GCGGGGGGCC TGGGCCAGGC
    ATGATGTGTG GGGCTGTCAA CAACCTGCTA AGTGACACGG AAGAAGATGA CAAATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90478
    Clone ID Related Accession (Same CDS sequence) XM_017002248.1
    Accession Version XM_017002248.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7311bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC
    ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG
    TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG
    GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC
    TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC
    CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC
    CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC
    CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT
    CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG
    CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA
    GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA
    GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA
    GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG
    GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG
    GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG
    GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC
    ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC
    GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA
    GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG
    CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC
    TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT
    GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC
    GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC
    GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG
    TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG
    GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG
    TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC
    AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG
    TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC
    TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT
    CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC
    GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT
    CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC
    CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC
    AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG
    GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT
    CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG
    GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG
    ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC
    GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG
    AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA
    GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG
    AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT
    TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GTATTTGGAA ACATAAAATT AGACGAGGAG
    AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTAAT GCTACTCTTC
    AGGAGTGCCA CAGGTGAGGC CTGGCAGGAG ATTATGCTGT CATGCCTTGG GGAGAAGGGC
    TGTGAGCCTG ACACCACCGC ACCATCAGGG CAGAACGAGA ACGAACGCTG CGGCACCGAT
    CTGGCCTACG TGTACTTTGT CTCCTTCATC TTCTTCTGCT CCTTCTTGAT GCTCAACCTG
    TTTGTGGCCG TCATCATGGA CAACTTTGAG TACCTGACTC GGGACTCCTC CATCCTGGGG
    CCTCACCACT TGGACGAGTT TGTCCGCGTC TGGGCAGAAT ATGACCGAGC AGCATGTGGC
    CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCTCC GCTAGGCCTC
    GGCAAGAGAT GTCCCTCCAA AGTGGCATAT AAGAGGTTGG TCCTGATGAA CATGCCAGTA
    GCTGAGGACA TGACGGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG
    GACATTAAAA TTGCCAAAGG TGGTGCAGAC AGGCAGCAGC TAGACTCAGA GCTACAAAAG
    GAGACCCTAG CCATCTGGCC TCACCTATCC CAGAAGATGC TGGATCTGCT TGTGCCCATG
    CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC
    TATAAGCAGA GTAAGGTGAA GAAGCAGAGG CAGCAGCTGG AGGAACAGAA AAATGCCCCC
    ATGTTCCAGC GCATGGAGCC TTCATCTCTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC
    CTGCCTTACC TCCAGCAGGA CCCCGTTTCA GGCCTGAGTG GCCGGAGTGG ATACCCTTCG
    ATGAGTCCAC TCTCTCCCCA GGATATATTC CAGTTGGCTT GTATGGACCC CGCCGATGAC
    GGACAGTTCC AAGAACGGCA GTCTCTGGAG CCAGAGGTTA GTGAATTAAA AAGCGTGCAG
    CCCTCTAACC ATGGCATCTA CCTTCCTTCG GACACCCAGG AGCATGCGGG ATCTGGGAGG
    GCATCTTCTA TGCCACGTCT GACTGTGGAT CCCCAGGTGG TGACAGACCC TAGCTCCATG
    AGACGTTCAT TTTCCACTAT TCGGGATAAG CGTTCAAATT CCTCGTGGTT GGAGGAATTC
    TCCATGGAGC GAAGCAGTGA AAATACCTAC AAGTCCCGTC GCCGGAGTTA CCACTCCTCC
    TTGCGGCTGT CAGCCCACCG CCTGAACTCT GATTCAGGCC ACAAGTCTGA CACTCACCGC
    TCAGGGGGCA GGGAGCGGGG ACGATCAAAA GAGCGAAAGC ATCTTCTCTC TCCTGATGTC
    TCCCGCTGCA ATTCAGAAGA GCGAGGGACC CAGGCTGACT GGGAGTCCCC AGAGCGCCGT
    CAATCCAGGT CACCCAGTGA GGGCAGGTCA CAGACGCCCA ACAGACAGGG CACAGGTTCC
    CTAAGTGAGA GCTCCATCCC CTCTGTCTCT GACACCAGCA CCCCAAGAAG AAGTCGTCGG
    CAGCTCCCAC CCGTCCCGCC AAAGCCCCGG CCCCTCCTTT CCTACAGCTC CCTGATTCGA
    CACGCGGGCA GCATCTCTCC ACCTGCTGAT GGAAGCGAGG AGGGCTCCCC GCTGACCTCC
    CAAGCTCTGG AGAGCAACAA TGCTTGCCTG ACCGAGTCTT CCAACTCTCC GCACCCCCAG
    CAGAGCCAAC ATGCCTCCCC ACAGCGCTAC ATCTCCGAGC CCTACTTGGC CCTGCACGAA
    GACTCCCACG CCTCAGACTG TGGTGAGGAG GAGACGCTCA CTTTCGAAGC AGCCGTGGCT
    ACTAGCCTGG GCCGTTCCAA CACCATCGGC TCAGCCCCAC CCCTGCGGCA TAGCTGGCAG
    ATGCCCAACG GGCACTATCG GCGGCGGAGG CGCGGGGGGC CTGGGCCAGG CATGATGTGT
    GGGGCTGTCA ACAACCTGCT AAGTGACACG GAAGAAGATG ACAAATGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016857737.1
    CDS427..7737
    Translation

    Target ORF information:

    RefSeq Version XM_017002248.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002248.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC
    ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG
    TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG
    GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC
    TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC
    CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC
    CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC
    CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT
    CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG
    CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA
    GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA
    GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA
    GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG
    GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG
    GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG
    GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC
    ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC
    GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA
    GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG
    CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC
    TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT
    GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC
    GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC
    GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG
    TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG
    GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG
    TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC
    AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG
    TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC
    TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT
    CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC
    GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT
    CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC
    CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC
    AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG
    GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT
    CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG
    GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG
    ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC
    GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG
    AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA
    GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG
    AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT
    TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GTATTTGGAA ACATAAAATT AGACGAGGAG
    AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTAAT GCTACTCTTC
    AGGAGTGCCA CAGGTGAGGC CTGGCAGGAG ATTATGCTGT CATGCCTTGG GGAGAAGGGC
    TGTGAGCCTG ACACCACCGC ACCATCAGGG CAGAACGAGA ACGAACGCTG CGGCACCGAT
    CTGGCCTACG TGTACTTTGT CTCCTTCATC TTCTTCTGCT CCTTCTTGAT GCTCAACCTG
    TTTGTGGCCG TCATCATGGA CAACTTTGAG TACCTGACTC GGGACTCCTC CATCCTGGGG
    CCTCACCACT TGGACGAGTT TGTCCGCGTC TGGGCAGAAT ATGACCGAGC AGCATGTGGC
    CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCTCC GCTAGGCCTC
    GGCAAGAGAT GTCCCTCCAA AGTGGCATAT AAGAGGTTGG TCCTGATGAA CATGCCAGTA
    GCTGAGGACA TGACGGTCCA CTTCACCTCC ACACTTATGG CTCTGATCCG GACAGCTCTG
    GACATTAAAA TTGCCAAAGG TGGTGCAGAC AGGCAGCAGC TAGACTCAGA GCTACAAAAG
    GAGACCCTAG CCATCTGGCC TCACCTATCC CAGAAGATGC TGGATCTGCT TGTGCCCATG
    CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC
    TATAAGCAGA GTAAGGTGAA GAAGCAGAGG CAGCAGCTGG AGGAACAGAA AAATGCCCCC
    ATGTTCCAGC GCATGGAGCC TTCATCTCTG CCTCAGGAGA TCATTGCTAA TGCCAAAGCC
    CTGCCTTACC TCCAGCAGGA CCCCGTTTCA GGCCTGAGTG GCCGGAGTGG ATACCCTTCG
    ATGAGTCCAC TCTCTCCCCA GGATATATTC CAGTTGGCTT GTATGGACCC CGCCGATGAC
    GGACAGTTCC AAGAACGGCA GTCTCTGGAG CCAGAGGTTA GTGAATTAAA AAGCGTGCAG
    CCCTCTAACC ATGGCATCTA CCTTCCTTCG GACACCCAGG AGCATGCGGG ATCTGGGAGG
    GCATCTTCTA TGCCACGTCT GACTGTGGAT CCCCAGGTGG TGACAGACCC TAGCTCCATG
    AGACGTTCAT TTTCCACTAT TCGGGATAAG CGTTCAAATT CCTCGTGGTT GGAGGAATTC
    TCCATGGAGC GAAGCAGTGA AAATACCTAC AAGTCCCGTC GCCGGAGTTA CCACTCCTCC
    TTGCGGCTGT CAGCCCACCG CCTGAACTCT GATTCAGGCC ACAAGTCTGA CACTCACCGC
    TCAGGGGGCA GGGAGCGGGG ACGATCAAAA GAGCGAAAGC ATCTTCTCTC TCCTGATGTC
    TCCCGCTGCA ATTCAGAAGA GCGAGGGACC CAGGCTGACT GGGAGTCCCC AGAGCGCCGT
    CAATCCAGGT CACCCAGTGA GGGCAGGTCA CAGACGCCCA ACAGACAGGG CACAGGTTCC
    CTAAGTGAGA GCTCCATCCC CTCTGTCTCT GACACCAGCA CCCCAAGAAG AAGTCGTCGG
    CAGCTCCCAC CCGTCCCGCC AAAGCCCCGG CCCCTCCTTT CCTACAGCTC CCTGATTCGA
    CACGCGGGCA GCATCTCTCC ACCTGCTGAT GGAAGCGAGG AGGGCTCCCC GCTGACCTCC
    CAAGCTCTGG AGAGCAACAA TGCTTGCCTG ACCGAGTCTT CCAACTCTCC GCACCCCCAG
    CAGAGCCAAC ATGCCTCCCC ACAGCGCTAC ATCTCCGAGC CCTACTTGGC CCTGCACGAA
    GACTCCCACG CCTCAGACTG TGGTGAGGAG GAGACGCTCA CTTTCGAAGC AGCCGTGGCT
    ACTAGCCTGG GCCGTTCCAA CACCATCGGC TCAGCCCCAC CCCTGCGGCA TAGCTGGCAG
    ATGCCCAACG GGCACTATCG GCGGCGGAGG CGCGGGGGGC CTGGGCCAGG CATGATGTGT
    GGGGCTGTCA ACAACCTGCT AAGTGACACG GAAGAAGATG ACAAATGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90479
    Clone ID Related Accession (Same CDS sequence) XM_017002249.1
    Accession Version XM_017002249.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7248bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC
    ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG
    TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG
    GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC
    TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC
    CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC
    CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC
    CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT
    CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG
    CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA
    GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA
    GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA
    GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG
    GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG
    GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG
    GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC
    ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC
    GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA
    GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG
    CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC
    TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT
    GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC
    GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC
    GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG
    TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG
    GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG
    TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC
    AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG
    TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC
    TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT
    CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC
    GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT
    CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC
    CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC
    AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG
    GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT
    CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG
    GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG
    ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC
    GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG
    AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA
    GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG
    AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT
    TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GCCCTCCCTT ATGTCTGCCT TTTAATTGCC
    ATGCTTTTCT TCATTTATGC CATCATTGGG ATGCAGGTAT TTGGAAACAT AAAATTAGAC
    GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGGTC CCTAATGCTA
    CTCTTCAGGA GTGCCACAGG TGAGGCCTGG CAGGAGATTA TGCTGTCATG CCTTGGGGAG
    AAGGGCTGTG AGCCTGACAC CACCGCACCA TCAGGGCAGA ACGAGAACGA ACGCTGCGGC
    ACCGATCTGG CCTACGTGTA CTTTGTCTCC TTCATCTTCT TCTGCTCCTT CTTGATGCTC
    AACCTGTTTG TGGCCGTCAT CATGGACAAC TTTGAGTACC TGACTCGGGA CTCCTCCATC
    CTGGGGCCTC ACCACTTGGA CGAGTTTGTC CGCGTCTGGG CAGAATATGA CCGAGCAGCA
    TGTGGCCGCA TCCATTACAC TGAGATGTAT GAAATGCTGA CTCTCATGTC ACCTCCGCTA
    GGCCTCGGCA AGAGATGTCC CTCCAAAGTG GCATATAAGA GGTTGGTCCT GATGAACATG
    CCAGTAGCTG AGGACATGAC GGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA
    GCTCTGGACA TTAAAATTGC CAAAGGTGGT GCAGACAGGC AGCAGCTAGA CTCAGAGCTA
    CAAAAGGAGA CCCTAGCCAT CTGGCCTCAC CTATCCCAGA AGATGCTGGA TCTGCTTGTG
    CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAAATCT ATGCAGCAAT GATGATCATG
    GACTACTATA AGCAGAGTAA GGTGAAGAAG CAGAGGCAGC AGCTGGAGGA ACAGAAAAAT
    GCCCCCATGT TCCAGCGCAT GGAGCCTTCA TCTCTGCCTC AGGAGATCAT TGCTAATGCC
    AAAGCCCTGC CTTACCTCCA GCAGGACCCC GTTTCAGGCC TGAGTGGCCG GAGTGGATAC
    CCTTCGATGA GTCCACTCTC TCCCCAGGAT ATATTCCAGT TGGCTTGTAT GGACCCCGCC
    GATGACGGAC AGTTCCAAGA ACGGCAGTCT CTGGTGGTGA CAGACCCTAG CTCCATGAGA
    CGTTCATTTT CCACTATTCG GGATAAGCGT TCAAATTCCT CGTGGTTGGA GGAATTCTCC
    ATGGAGCGAA GCAGTGAAAA TACCTACAAG TCCCGTCGCC GGAGTTACCA CTCCTCCTTG
    CGGCTGTCAG CCCACCGCCT GAACTCTGAT TCAGGCCACA AGTCTGACAC TCACCGCTCA
    GGGGGCAGGG AGCGGGGACG ATCAAAAGAG CGAAAGCATC TTCTCTCTCC TGATGTCTCC
    CGCTGCAATT CAGAAGAGCG AGGGACCCAG GCTGACTGGG AGTCCCCAGA GCGCCGTCAA
    TCCAGGTCAC CCAGTGAGGG CAGGTCACAG ACGCCCAACA GACAGGGCAC AGGTTCCCTA
    AGTGAGAGCT CCATCCCCTC TGTCTCTGAC ACCAGCACCC CAAGAAGAAG TCGTCGGCAG
    CTCCCACCCG TCCCGCCAAA GCCCCGGCCC CTCCTTTCCT ACAGCTCCCT GATTCGACAC
    GCGGGCAGCA TCTCTCCACC TGCTGATGGA AGCGAGGAGG GCTCCCCGCT GACCTCCCAA
    GCTCTGGAGA GCAACAATGC TTGCCTGACC GAGTCTTCCA ACTCTCCGCA CCCCCAGCAG
    AGCCAACATG CCTCCCCACA GCGCTACATC TCCGAGCCCT ACTTGGCCCT GCACGAAGAC
    TCCCACGCCT CAGACTGTGG TGAGGAGGAG ACGCTCACTT TCGAAGCAGC CGTGGCTACT
    AGCCTGGGCC GTTCCAACAC CATCGGCTCA GCCCCACCCC TGCGGCATAG CTGGCAGATG
    CCCAACGGGC ACTATCGGCG GCGGAGGCGC GGGGGGCCTG GGCCAGGCAT GATGTGTGGG
    GCTGTCAACA ACCTGCTAAG TGACACGGAA GAAGATGACA AATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016857738.1
    CDS426..7673
    Translation

    Target ORF information:

    RefSeq Version XM_017002249.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002249.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAAAGAG ACAGAAGGAG AAGACACCAC
    ATGTCGATGT GGGAGCCACG CAGCAGCCAC CTGAGGGAGC GGAGGCGCCG GCACCACATG
    TCCGTGTGGG AGCAGCGTAC CAGCCAGCTG AGGAAGCACA TGCAGATGTC CAGCCAGGAG
    GCCCTCAACA GAGAGGAGGC GCCGACCATG AACCCGCTCA ACCCCCTCAA CCCGCTCAGC
    TCCCTCAACC CGCTCAATGC CCACCCCAGC CTTTATCGGC GACCCAGGGC CATTGAGGGC
    CTGGCCCTGG GCCTGGCCCT GGAGAAGTTC GAGGAGGAGC GCATCAGCCG TGGGGGGTCC
    CTCAAGGGGG ATGGAGGGGA CCGATCCAGT GCCCTGGACA ACCAGAGGAC CCCTTTGTCC
    CTGGGCCAGC GGGAGCCACC ATGGCTGGCC AGGCCCTGTC ATGGAAACTG TGACCCGACT
    CAGCAGGAGG CAGGGGGAGG AGAGGCTGTG GTGACCTTTG AGGACCGGGC CAGGCACAGG
    CAGAGCCAAC GGCGCAGCCG GCATCGCCGC GTCAGGACAG AAGGCAAGGA GTCCTCTTCA
    GCCTCCCGGA GCAGGTCTGC CAGCCAGGAA CGCAGTCTGG ATGAAGCCAT GCCCACTGAA
    GGGGAGAAGG ACCATGAGCT CAGGGGCAAC CATGGTGCCA AGGAGCCAAC GATCCAAGAA
    GAGAGAGCCC AGGATTTAAG GAGGACCAAC AGTCTGATGG TGTCCAGAGG CTCCGGGCTG
    GCAGGAGGCC TTGATGAGGC TGACACCCCC CTAGTCCTGC CCCATCCTGA GCTGGAAGTG
    GGGAAGCACG TGGTGCTGAC GGAGCAGGAG CCAGAAGGCA GCAGTGAGCA GGCCCTGCTG
    GGGAATGTGC AGCTAGACAT GGGCCGGGTC ATCAGCCAGA GCGAGCCTGA CCTCTCCTGC
    ATCACGGCCA ACACGGACAA GGCCACCACC GAGAGCACCA GCGTCACCGT CGCCATCCCC
    GACGTGGACC CCTTGGTGGA CTCAACCGTG GTGCACATTA GCAACAAGAC GGATGGGGAA
    GCCAGTCCCT TGAAGGAGGC AGAGATCAGA GAGGATGAGG AGGAGGTGGA GAAGAAGAAG
    CAGAAGAAGG AGAAGCGTGA GACAGGCAAA GCCATGGTGC CCCACAGCTC AATGTTCATC
    TTCAGCACCA CCAACCCGAT CCGGAGGGCC TGCCACTACA TCGTGAACCT GCGCTACTTT
    GAGATGTGCA TCCTCCTGGT GATTGCAGCC AGCAGCATCG CCCTGGCGGC AGAGGACCCC
    GTCCTGACCA ACTCGGAGCG CAACAAAGTC CTGAGGTATT TTGACTATGT GTTCACGGGC
    GTGTTCACCT TTGAGATGGT TATAAAGATG ATAGACCAAG GCTTGATCCT GCAGGATGGG
    TCCTACTTCC GAGACTTGTG GAACATCCTG GACTTTGTGG TGGTCGTTGG CGCATTGGTG
    GCCTTTGCTC TGGCGAACGC TTTGGGAACC AACAAAGGAC GGGACATCAA GACCATCAAG
    TCTCTGCGGG TGCTCCGAGT TCTAAGGCCA CTGAAAACCA TCAAGCGCTT GCCCAAGCTC
    AAGGCCGTCT TCGACTGCGT AGTGACCTCC TTGAAGAATG TCTTCAACAT ACTCATTGTG
    TACAAGCTCT TCATGTTCAT CTTTGCTGTC ATCGCAGTTC AGCTCTTCAA GGGAAAGTTC
    TTTTATTGCA CGGACAGTTC CAAGGACACA GAGAAGGAGT GCATAGGCAA CTATGTAGAT
    CATGAGAAAA ACAAGATGGA GGTGAAGGGC CGGGAATGGA AGCGCCATGA ATTCCACTAC
    GACAACATTA TCTGGGCCCT GCTGACCCTC TTCACCGTCT CCACAGGGGA AGGATGGCCT
    CAAGTTCTGC AGCACTCTGT AGATGTGACA GAGGAAGACC GAGGCCCAAG CCGCAGCAAC
    CGCATGGAGA TGTCTATCTT TTATGTAGTC TACTTTGTGG TCTTCCCCTT CTTCTTTGTC
    AATATCTTTG TGGCTCTCAT CATCATCACC TTCCAGGAGC AAGGGGATAA GATGATGGAG
    GAGTGCAGCC TGGAGAAGAA TGAGAGGGCG TGCATCGACT TCGCCATCAG CGCCAAACCT
    CTCACCCGCT ACATGCCGCA GAACAGACAC ACCTTCCAGT ACCGCGTGTG GCACTTTGTG
    GTGTCTCCGT CCTTTGAGTA CACCATTATG GCCATGATCG CCTTGAATAC TGTTGTGCTG
    ATGATGAAGT ATTATTCTGC TCCCTGTACC TATGAGCTGG CCCTGAAGTA CCTGAATATC
    GCCTTCACCA TGGTGTTTTC CCTGGAATGT GTCCTGAAGG TCATCGCTTT TGGCTTTTTG
    AACTATTTCC GAGACACCTG GAATATCTTT GACTTCATCA CCGTGATTGG CAGTATCACA
    GAAATTATCC TGACAGACAG CAAGCTGGTG AACACCAGTG GCTTCAATAT GAGCTTTCTG
    AAGCTCTTCC GAGCTGCCCG CCTCATAAAG CTCCTGCGTC AGGGCTATAC CATACGCATT
    TTGCTGTGGA CCTTTGTGCA GTCCTTTAAG GCCCTCCCTT ATGTCTGCCT TTTAATTGCC
    ATGCTTTTCT TCATTTATGC CATCATTGGG ATGCAGGTAT TTGGAAACAT AAAATTAGAC
    GAGGAGAGTC ACATCAACCG GCACAACAAC TTCCGGAGTT TCTTTGGGTC CCTAATGCTA
    CTCTTCAGGA GTGCCACAGG TGAGGCCTGG CAGGAGATTA TGCTGTCATG CCTTGGGGAG
    AAGGGCTGTG AGCCTGACAC CACCGCACCA TCAGGGCAGA ACGAGAACGA ACGCTGCGGC
    ACCGATCTGG CCTACGTGTA CTTTGTCTCC TTCATCTTCT TCTGCTCCTT CTTGATGCTC
    AACCTGTTTG TGGCCGTCAT CATGGACAAC TTTGAGTACC TGACTCGGGA CTCCTCCATC
    CTGGGGCCTC ACCACTTGGA CGAGTTTGTC CGCGTCTGGG CAGAATATGA CCGAGCAGCA
    TGTGGCCGCA TCCATTACAC TGAGATGTAT GAAATGCTGA CTCTCATGTC ACCTCCGCTA
    GGCCTCGGCA AGAGATGTCC CTCCAAAGTG GCATATAAGA GGTTGGTCCT GATGAACATG
    CCAGTAGCTG AGGACATGAC GGTCCACTTC ACCTCCACAC TTATGGCTCT GATCCGGACA
    GCTCTGGACA TTAAAATTGC CAAAGGTGGT GCAGACAGGC AGCAGCTAGA CTCAGAGCTA
    CAAAAGGAGA CCCTAGCCAT CTGGCCTCAC CTATCCCAGA AGATGCTGGA TCTGCTTGTG
    CCCATGCCCA AAGCCTCTGA CCTGACTGTG GGCAAAATCT ATGCAGCAAT GATGATCATG
    GACTACTATA AGCAGAGTAA GGTGAAGAAG CAGAGGCAGC AGCTGGAGGA ACAGAAAAAT
    GCCCCCATGT TCCAGCGCAT GGAGCCTTCA TCTCTGCCTC AGGAGATCAT TGCTAATGCC
    AAAGCCCTGC CTTACCTCCA GCAGGACCCC GTTTCAGGCC TGAGTGGCCG GAGTGGATAC
    CCTTCGATGA GTCCACTCTC TCCCCAGGAT ATATTCCAGT TGGCTTGTAT GGACCCCGCC
    GATGACGGAC AGTTCCAAGA ACGGCAGTCT CTGGTGGTGA CAGACCCTAG CTCCATGAGA
    CGTTCATTTT CCACTATTCG GGATAAGCGT TCAAATTCCT CGTGGTTGGA GGAATTCTCC
    ATGGAGCGAA GCAGTGAAAA TACCTACAAG TCCCGTCGCC GGAGTTACCA CTCCTCCTTG
    CGGCTGTCAG CCCACCGCCT GAACTCTGAT TCAGGCCACA AGTCTGACAC TCACCGCTCA
    GGGGGCAGGG AGCGGGGACG ATCAAAAGAG CGAAAGCATC TTCTCTCTCC TGATGTCTCC
    CGCTGCAATT CAGAAGAGCG AGGGACCCAG GCTGACTGGG AGTCCCCAGA GCGCCGTCAA
    TCCAGGTCAC CCAGTGAGGG CAGGTCACAG ACGCCCAACA GACAGGGCAC AGGTTCCCTA
    AGTGAGAGCT CCATCCCCTC TGTCTCTGAC ACCAGCACCC CAAGAAGAAG TCGTCGGCAG
    CTCCCACCCG TCCCGCCAAA GCCCCGGCCC CTCCTTTCCT ACAGCTCCCT GATTCGACAC
    GCGGGCAGCA TCTCTCCACC TGCTGATGGA AGCGAGGAGG GCTCCCCGCT GACCTCCCAA
    GCTCTGGAGA GCAACAATGC TTGCCTGACC GAGTCTTCCA ACTCTCCGCA CCCCCAGCAG
    AGCCAACATG CCTCCCCACA GCGCTACATC TCCGAGCCCT ACTTGGCCCT GCACGAAGAC
    TCCCACGCCT CAGACTGTGG TGAGGAGGAG ACGCTCACTT TCGAAGCAGC CGTGGCTACT
    AGCCTGGGCC GTTCCAACAC CATCGGCTCA GCCCCACCCC TGCGGCATAG CTGGCAGATG
    CCCAACGGGC ACTATCGGCG GCGGAGGCGC GGGGGGCCTG GGCCAGGCAT GATGTGTGGG
    GCTGTCAACA ACCTGCTAAG TGACACGGAA GAAGATGACA AATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90480
    Clone ID Related Accession (Same CDS sequence) XM_017002250.1
    Accession Version XM_017002250.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7191bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAGAGGG AGCGGAGGCG CCGGCACCAC
    ATGTCCGTGT GGGAGCAGCG TACCAGCCAG CTGAGGAAGC ACATGCAGAT GTCCAGCCAG
    GAGGCCCTCA ACAGAGAGGA GGCGCCGACC ATGAACCCGC TCAACCCCCT CAACCCGCTC
    AGCTCCCTCA ACCCGCTCAA TGCCCACCCC AGCCTTTATC GGCGACCCAG GGCCATTGAG
    GGCCTGGCCC TGGGCCTGGC CCTGGAGAAG TTCGAGGAGG AGCGCATCAG CCGTGGGGGG
    TCCCTCAAGG GGGATGGAGG GGACCGATCC AGTGCCCTGG ACAACCAGAG GACCCCTTTG
    TCCCTGGGCC AGCGGGAGCC ACCATGGCTG GCCAGGCCCT GTCATGGAAA CTGTGACCCG
    ACTCAGCAGG AGGCAGGGGG AGGAGAGGCT GTGGTGACCT TTGAGGACCG GGCCAGGCAC
    AGGCAGAGCC AACGGCGCAG CCGGCATCGC CGCGTCAGGA CAGAAGGCAA GGAGTCCTCT
    TCAGCCTCCC GGAGCAGGTC TGCCAGCCAG GAACGCAGTC TGGATGAAGC CATGCCCACT
    GAAGGGGAGA AGGACCATGA GCTCAGGGGC AACCATGGTG CCAAGGAGCC AACGATCCAA
    GAAGAGAGAG CCCAGGATTT AAGGAGGACC AACAGTCTGA TGGTGTCCAG AGGCTCCGGG
    CTGGCAGGAG GCCTTGATGA GGCTGACACC CCCCTAGTCC TGCCCCATCC TGAGCTGGAA
    GTGGGGAAGC ACGTGGTGCT GACGGAGCAG GAGCCAGAAG GCAGCAGTGA GCAGGCCCTG
    CTGGGGAATG TGCAGCTAGA CATGGGCCGG GTCATCAGCC AGAGCGAGCC TGACCTCTCC
    TGCATCACGG CCAACACGGA CAAGGCCACC ACCGAGAGCA CCAGCGTCAC CGTCGCCATC
    CCCGACGTGG ACCCCTTGGT GGACTCAACC GTGGTGCACA TTAGCAACAA GACGGATGGG
    GAAGCCAGTC CCTTGAAGGA GGCAGAGATC AGAGAGGATG AGGAGGAGGT GGAGAAGAAG
    AAGCAGAAGA AGGAGAAGCG TGAGACAGGC AAAGCCATGG TGCCCCACAG CTCAATGTTC
    ATCTTCAGCA CCACCAACCC GATCCGGAGG GCCTGCCACT ACATCGTGAA CCTGCGCTAC
    TTTGAGATGT GCATCCTCCT GGTGATTGCA GCCAGCAGCA TCGCCCTGGC GGCAGAGGAC
    CCCGTCCTGA CCAACTCGGA GCGCAACAAA GTCCTGAGGT ATTTTGACTA TGTGTTCACG
    GGCGTGTTCA CCTTTGAGAT GGTTATAAAG ATGATAGACC AAGGCTTGAT CCTGCAGGAT
    GGGTCCTACT TCCGAGACTT GTGGAACATC CTGGACTTTG TGGTGGTCGT TGGCGCATTG
    GTGGCCTTTG CTCTGGCGAA CGCTTTGGGA ACCAACAAAG GACGGGACAT CAAGACCATC
    AAGTCTCTGC GGGTGCTCCG AGTTCTAAGG CCACTGAAAA CCATCAAGCG CTTGCCCAAG
    CTCAAGGCCG TCTTCGACTG CGTAGTGACC TCCTTGAAGA ATGTCTTCAA CATACTCATT
    GTGTACAAGC TCTTCATGTT CATCTTTGCT GTCATCGCAG TTCAGCTCTT CAAGGGAAAG
    TTCTTTTATT GCACGGACAG TTCCAAGGAC ACAGAGAAGG AGTGCATAGG CAACTATGTA
    GATCATGAGA AAAACAAGAT GGAGGTGAAG GGCCGGGAAT GGAAGCGCCA TGAATTCCAC
    TACGACAACA TTATCTGGGC CCTGCTGACC CTCTTCACCG TCTCCACAGG GGAAGGATGG
    CCTCAAGTTC TGCAGCACTC TGTAGATGTG ACAGAGGAAG ACCGAGGCCC AAGCCGCAGC
    AACCGCATGG AGATGTCTAT CTTTTATGTA GTCTACTTTG TGGTCTTCCC CTTCTTCTTT
    GTCAATATCT TTGTGGCTCT CATCATCATC ACCTTCCAGG AGCAAGGGGA TAAGATGATG
    GAGGAGTGCA GCCTGGAGAA GAATGAGAGG GCGTGCATCG ACTTCGCCAT CAGCGCCAAA
    CCTCTCACCC GCTACATGCC GCAGAACAGA CACACCTTCC AGTACCGCGT GTGGCACTTT
    GTGGTGTCTC CGTCCTTTGA GTACACCATT ATGGCCATGA TCGCCTTGAA TACTGTTGTG
    CTGATGATGA AGTATTATTC TGCTCCCTGT ACCTATGAGC TGGCCCTGAA GTACCTGAAT
    ATCGCCTTCA CCATGGTGTT TTCCCTGGAA TGTGTCCTGA AGGTCATCGC TTTTGGCTTT
    TTGAACTATT TCCGAGACAC CTGGAATATC TTTGACTTCA TCACCGTGAT TGGCAGTATC
    ACAGAAATTA TCCTGACAGA CAGCAAGCTG GTGAACACCA GTGGCTTCAA TATGAGCTTT
    CTGAAGCTCT TCCGAGCTGC CCGCCTCATA AAGCTCCTGC GTCAGGGCTA TACCATACGC
    ATTTTGCTGT GGACCTTTGT GCAGTCCTTT AAGGCCCTCC CTTATGTCTG CCTTTTAATT
    GCCATGCTTT TCTTCATTTA TGCCATCATT GGGATGCAGG TATTTGGAAA CATAAAATTA
    GACGAGGAGA GTCACATCAA CCGGCACAAC AACTTCCGGA GTTTCTTTGG GTCCCTAATG
    CTACTCTTCA GGAGTGCCAC AGGTGAGGCC TGGCAGGAGA TTATGCTGTC ATGCCTTGGG
    GAGAAGGGCT GTGAGCCTGA CACCACCGCA CCATCAGGGC AGAACGAGAA CGAACGCTGC
    GGCACCGATC TGGCCTACGT GTACTTTGTC TCCTTCATCT TCTTCTGCTC CTTCTTGATG
    CTCAACCTGT TTGTGGCCGT CATCATGGAC AACTTTGAGT ACCTGACTCG GGACTCCTCC
    ATCCTGGGGC CTCACCACTT GGACGAGTTT GTCCGCGTCT GGGCAGAATA TGACCGAGCA
    GCATGTGGCC GCATCCATTA CACTGAGATG TATGAAATGC TGACTCTCAT GTCACCTCCG
    CTAGGCCTCG GCAAGAGATG TCCCTCCAAA GTGGCATATA AGAGGTTGGT CCTGATGAAC
    ATGCCAGTAG CTGAGGACAT GACGGTCCAC TTCACCTCCA CACTTATGGC TCTGATCCGG
    ACAGCTCTGG ACATTAAAAT TGCCAAAGGT GGTGCAGACA GGCAGCAGCT AGACTCAGAG
    CTACAAAAGG AGACCCTAGC CATCTGGCCT CACCTATCCC AGAAGATGCT GGATCTGCTT
    GTGCCCATGC CCAAAGCCTC TGACCTGACT GTGGGCAAAA TCTATGCAGC AATGATGATC
    ATGGACTACT ATAAGCAGAG TAAGGTGAAG AAGCAGAGGC AGCAGCTGGA GGAACAGAAA
    AATGCCCCCA TGTTCCAGCG CATGGAGCCT TCATCTCTGC CTCAGGAGAT CATTGCTAAT
    GCCAAAGCCC TGCCTTACCT CCAGCAGGAC CCCGTTTCAG GCCTGAGTGG CCGGAGTGGA
    TACCCTTCGA TGAGTCCACT CTCTCCCCAG GATATATTCC AGTTGGCTTG TATGGACCCC
    GCCGATGACG GACAGTTCCA AGAACGGCAG TCTCTGGTGG TGACAGACCC TAGCTCCATG
    AGACGTTCAT TTTCCACTAT TCGGGATAAG CGTTCAAATT CCTCGTGGTT GGAGGAATTC
    TCCATGGAGC GAAGCAGTGA AAATACCTAC AAGTCCCGTC GCCGGAGTTA CCACTCCTCC
    TTGCGGCTGT CAGCCCACCG CCTGAACTCT GATTCAGGCC ACAAGTCTGA CACTCACCGC
    TCAGGGGGCA GGGAGCGGGG ACGATCAAAA GAGCGAAAGC ATCTTCTCTC TCCTGATGTC
    TCCCGCTGCA ATTCAGAAGA GCGAGGGACC CAGGCTGACT GGGAGTCCCC AGAGCGCCGT
    CAATCCAGGT CACCCAGTGA GGGCAGGTCA CAGACGCCCA ACAGACAGGG CACAGGTTCC
    CTAAGTGAGA GCTCCATCCC CTCTGTCTCT GACACCAGCA CCCCAAGAAG AAGTCGTCGG
    CAGCTCCCAC CCGTCCCGCC AAAGCCCCGG CCCCTCCTTT CCTACAGCTC CCTGATTCGA
    CACGCGGGCA GCATCTCTCC ACCTGCTGAT GGAAGCGAGG AGGGCTCCCC GCTGACCTCC
    CAAGCTCTGG AGAGCAACAA TGCTTGCCTG ACCGAGTCTT CCAACTCTCC GCACCCCCAG
    CAGAGCCAAC ATGCCTCCCC ACAGCGCTAC ATCTCCGAGC CCTACTTGGC CCTGCACGAA
    GACTCCCACG CCTCAGACTG TGGTGAGGAG GAGACGCTCA CTTTCGAAGC AGCCGTGGCT
    ACTAGCCTGG GCCGTTCCAA CACCATCGGC TCAGCCCCAC CCCTGCGGCA TAGCTGGCAG
    ATGCCCAACG GGCACTATCG GCGGCGGAGG CGCGGGGGGC CTGGGCCAGG CATGATGTGT
    GGGGCTGTCA ACAACCTGCT AAGTGACACG GAAGAAGATG ACAAATGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016857739.1
    CDS426..7616
    Translation

    Target ORF information:

    RefSeq Version XM_017002250.1
    Organism Homo sapiens(human)
    Definition Homo sapiens calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017002250.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGGTGCCCG AGATGCGTGG ACTGAGCCCA GACCGCAGGC TGCCCGAGCC CGCCCCCTGC 
    CCGGCTGCGG GGAGCCCTGA GCCCAGGTCC GCGGGCCGCC GCCAACGCCA CGTCCTGGCC
    CTGCCGGAAG AAGAGGCATG GCGGCCCCAG CAGTGCGCTC TGCTCGAGGC CGACGCCTCG
    GACGAGGTGG GCATGCTGCC GGCTTCCCCG CGCGCCGCCT CTGGCTTCGA CAACTTCTTC
    CAGGTGCAGG AGGGCGAGGG CCAGGGCTGG GAGGGCGCCA TGGCACTGGA GGCGGGCTCC
    AGCCCTTTCC TGCCGGTGAG CCCCGAGGTC ATGAAGCGGC GGCGCGGGGG CCTCATCGAG
    CAGCGCGACA TCATCAAGGC CCACGAGGCG CACAAGATGC AGAGCACCCC GCAGGCCCGG
    CGCAAGGAAT GGGAGATGGC TCGCTTCGGG GAGGCGGTGG TCGCCAGGCC AGGGTCCGGC
    GATGGAGACT CGGACCAGAG CAGGAACCGG CAAGGAACCC CCGTGCCGGC CTCGGGGCAG
    GCGGCCGCCT ACAAGCAGAC GAAAGCACAG AGGGCGCGGA CTATGGCTTT GTACAACCCC
    ATTCCCGTCC GGCAGAACTG TTTCACCGTC AACAGATCCC TGTTCATCTT CGGAGAAGAT
    AACATTGTCA GGAAATATGC CAAGAAGCTC ATCGATTGGC CGCCATTTGA GTACATGATC
    CTGGCCACCA TCATTGCCAA CTGCATCGTC CTGGCCCTGG AGCAGCATCT TCCTGAGGAT
    GACAAGACCC CCATGTCCCG AAGACTGGAG AAGACAGAAC CTTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATTGTGGCC CTGGGGTTCA TCTTCCATAA GGGCTCTTAC
    CTCCGCAATG GCTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCCACT
    GCAGGAACCC ACTTCAATAC TCACGTGGAC CTGAGGACCC TCCGGGCTGT GCGTGTCCTG
    CGGCCTTTGA AGCTCGTGTC AGGGATACCT AGCCTGCAGA TTGTGTTGAA GTCCATCATG
    AAGGCCATGG TACCTCTTCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATTG GTTTGGAGTT CTACAGTGGC AAGTTACATC GAGCATGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GTGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCAAGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCTG TGCTGACTGT CTTCCAGTGC ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTA GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGAAGG GCTTTCATGA AGCTGCGGCG CCAGCAGCAG
    ATTGAGCGTG AGCTGAATGG CTACCGTGCC TGGATAGACA AAGCAGAGGA AGTCATGCTC
    GCTGAAGAAA ATAAAAATGC TGGAACATCC GCCTTAGAAG TGCTTCGAAG GGCAACCATC
    AAGAGGAGCC GGACAGAGGC CATGACTCGA GACTCCAGTG ATGAGCACTG TGTTGATATC
    TCCTCTGTGG GCACACCTCT GGCCCGAGCC AGTATCAAAA GTGCAAAGGT AGACGGGGTC
    TCTTATTTCC GGCACAAGGA AAGGCTTCTG CGCATCTCCA TTCGCCACAT GGTTAAATCC
    CAGGTGTTTT ACTGGATTGT GCTGAGCCTT GTGGCACTCA ACACTGCCTG TGTGGCCATT
    GTCCATCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA ATTTCTGTTT
    CTGGGACTCT TCCTCTTGGA GATGTCCCTG AAGATGTATG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CATTCAACTG CTTTGATTTT GGGGTCACAG TGGGCAGTAT CTTTGAAGTG
    GTCTGGGCAA TCTTCAGACC TGGTACGTCT TTTGGAATCA GTGTCTTGCG AGCCCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCTTCCC TACGGAATTT GGTGGTCTCC
    TTGATGAGCT CAATGAAGTC TATCATCAGT TTGCTTTTCC TCCTCTTCCT CTTCATCGTT
    GTCTTTGCTC TCCTAGGAAT GCAGTTATTT GGAGGCAGGT TTAACTTTAA TGATGGGACT
    CCTTCGGCAA ATTTTGATAC CTTCCCTGCA GCCATCATGA CTGTGTTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAT GGGATCCGCT CCCAGGGTGG GGTCAGCTCA
    GGCATGTGGT CTGCCATCTA CTTCATTGTG CTCACCTTGT TTGGCAACTA CACGCTACTG
    AATGTGTTCT TGGCTATCGC TGTGGATAAT CTCGCCAACG CCCAGGAACT GACCAAGGAT
    GAACAGGAGG AAGAAGAGGC CTTCAACCAG AAACATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCG ATCGAGAGGG AGCGGAGGCG CCGGCACCAC
    ATGTCCGTGT GGGAGCAGCG TACCAGCCAG CTGAGGAAGC ACATGCAGAT GTCCAGCCAG
    GAGGCCCTCA ACAGAGAGGA GGCGCCGACC ATGAACCCGC TCAACCCCCT CAACCCGCTC
    AGCTCCCTCA ACCCGCTCAA TGCCCACCCC AGCCTTTATC GGCGACCCAG GGCCATTGAG
    GGCCTGGCCC TGGGCCTGGC CCTGGAGAAG TTCGAGGAGG AGCGCATCAG CCGTGGGGGG
    TCCCTCAAGG GGGATGGAGG GGACCGATCC AGTGCCCTGG ACAACCAGAG GACCCCTTTG
    TCCCTGGGCC AGCGGGAGCC ACCATGGCTG GCCAGGCCCT GTCATGGAAA CTGTGACCCG
    ACTCAGCAGG AGGCAGGGGG AGGAGAGGCT GTGGTGACCT TTGAGGACCG GGCCAGGCAC
    AGGCAGAGCC AACGGCGCAG CCGGCATCGC CGCGTCAGGA CAGAAGGCAA GGAGTCCTCT
    TCAGCCTCCC GGAGCAGGTC TGCCAGCCAG GAACGCAGTC TGGATGAAGC CATGCCCACT
    GAAGGGGAGA AGGACCATGA GCTCAGGGGC AACCATGGTG CCAAGGAGCC AACGATCCAA
    GAAGAGAGAG CCCAGGATTT AAGGAGGACC AACAGTCTGA TGGTGTCCAG AGGCTCCGGG
    CTGGCAGGAG GCCTTGATGA GGCTGACACC CCCCTAGTCC TGCCCCATCC TGAGCTGGAA
    GTGGGGAAGC ACGTGGTGCT GACGGAGCAG GAGCCAGAAG GCAGCAGTGA GCAGGCCCTG
    CTGGGGAATG TGCAGCTAGA CATGGGCCGG GTCATCAGCC AGAGCGAGCC TGACCTCTCC
    TGCATCACGG CCAACACGGA CAAGGCCACC ACCGAGAGCA CCAGCGTCAC CGTCGCCATC
    CCCGACGTGG ACCCCTTGGT GGACTCAACC GTGGTGCACA TTAGCAACAA GACGGATGGG
    GAAGCCAGTC CCTTGAAGGA GGCAGAGATC AGAGAGGATG AGGAGGAGGT GGAGAAGAAG
    AAGCAGAAGA AGGAGAAGCG TGAGACAGGC AAAGCCATGG TGCCCCACAG CTCAATGTTC
    ATCTTCAGCA CCACCAACCC GATCCGGAGG GCCTGCCACT ACATCGTGAA CCTGCGCTAC
    TTTGAGATGT GCATCCTCCT GGTGATTGCA GCCAGCAGCA TCGCCCTGGC GGCAGAGGAC
    CCCGTCCTGA CCAACTCGGA GCGCAACAAA GTCCTGAGGT ATTTTGACTA TGTGTTCACG
    GGCGTGTTCA CCTTTGAGAT GGTTATAAAG ATGATAGACC AAGGCTTGAT CCTGCAGGAT
    GGGTCCTACT TCCGAGACTT GTGGAACATC CTGGACTTTG TGGTGGTCGT TGGCGCATTG
    GTGGCCTTTG CTCTGGCGAA CGCTTTGGGA ACCAACAAAG GACGGGACAT CAAGACCATC
    AAGTCTCTGC GGGTGCTCCG AGTTCTAAGG CCACTGAAAA CCATCAAGCG CTTGCCCAAG
    CTCAAGGCCG TCTTCGACTG CGTAGTGACC TCCTTGAAGA ATGTCTTCAA CATACTCATT
    GTGTACAAGC TCTTCATGTT CATCTTTGCT GTCATCGCAG TTCAGCTCTT CAAGGGAAAG
    TTCTTTTATT GCACGGACAG TTCCAAGGAC ACAGAGAAGG AGTGCATAGG CAACTATGTA
    GATCATGAGA AAAACAAGAT GGAGGTGAAG GGCCGGGAAT GGAAGCGCCA TGAATTCCAC
    TACGACAACA TTATCTGGGC CCTGCTGACC CTCTTCACCG TCTCCACAGG GGAAGGATGG
    CCTCAAGTTC TGCAGCACTC TGTAGATGTG ACAGAGGAAG ACCGAGGCCC AAGCCGCAGC
    AACCGCATGG AGATGTCTAT CTTTTATGTA GTCTACTTTG TGGTCTTCCC CTTCTTCTTT
    GTCAATATCT TTGTGGCTCT CATCATCATC ACCTTCCAGG AGCAAGGGGA TAAGATGATG
    GAGGAGTGCA GCCTGGAGAA GAATGAGAGG GCGTGCATCG ACTTCGCCAT CAGCGCCAAA
    CCTCTCACCC GCTACATGCC GCAGAACAGA CACACCTTCC AGTACCGCGT GTGGCACTTT
    GTGGTGTCTC CGTCCTTTGA GTACACCATT ATGGCCATGA TCGCCTTGAA TACTGTTGTG
    CTGATGATGA AGTATTATTC TGCTCCCTGT ACCTATGAGC TGGCCCTGAA GTACCTGAAT
    ATCGCCTTCA CCATGGTGTT TTCCCTGGAA TGTGTCCTGA AGGTCATCGC TTTTGGCTTT
    TTGAACTATT TCCGAGACAC CTGGAATATC TTTGACTTCA TCACCGTGAT TGGCAGTATC
    ACAGAAATTA TCCTGACAGA CAGCAAGCTG GTGAACACCA GTGGCTTCAA TATGAGCTTT
    CTGAAGCTCT TCCGAGCTGC CCGCCTCATA AAGCTCCTGC GTCAGGGCTA TACCATACGC
    ATTTTGCTGT GGACCTTTGT GCAGTCCTTT AAGGCCCTCC CTTATGTCTG CCTTTTAATT
    GCCATGCTTT TCTTCATTTA TGCCATCATT GGGATGCAGG TATTTGGAAA CATAAAATTA
    GACGAGGAGA GTCACATCAA CCGGCACAAC AACTTCCGGA GTTTCTTTGG GTCCCTAATG
    CTACTCTTCA GGAGTGCCAC AGGTGAGGCC TGGCAGGAGA TTATGCTGTC ATGCCTTGGG
    GAGAAGGGCT GTGAGCCTGA CACCACCGCA CCATCAGGGC AGAACGAGAA CGAACGCTGC
    GGCACCGATC TGGCCTACGT GTACTTTGTC TCCTTCATCT TCTTCTGCTC CTTCTTGATG
    CTCAACCTGT TTGTGGCCGT CATCATGGAC AACTTTGAGT ACCTGACTCG GGACTCCTCC
    ATCCTGGGGC CTCACCACTT GGACGAGTTT GTCCGCGTCT GGGCAGAATA TGACCGAGCA
    GCATGTGGCC GCATCCATTA CACTGAGATG TATGAAATGC TGACTCTCAT GTCACCTCCG
    CTAGGCCTCG GCAAGAGATG TCCCTCCAAA GTGGCATATA AGAGGTTGGT CCTGATGAAC
    ATGCCAGTAG CTGAGGACAT GACGGTCCAC TTCACCTCCA CACTTATGGC TCTGATCCGG
    ACAGCTCTGG ACATTAAAAT TGCCAAAGGT GGTGCAGACA GGCAGCAGCT AGACTCAGAG
    CTACAAAAGG AGACCCTAGC CATCTGGCCT CACCTATCCC AGAAGATGCT GGATCTGCTT
    GTGCCCATGC CCAAAGCCTC TGACCTGACT GTGGGCAAAA TCTATGCAGC AATGATGATC
    ATGGACTACT ATAAGCAGAG TAAGGTGAAG AAGCAGAGGC AGCAGCTGGA GGAACAGAAA
    AATGCCCCCA TGTTCCAGCG CATGGAGCCT TCATCTCTGC CTCAGGAGAT CATTGCTAAT
    GCCAAAGCCC TGCCTTACCT CCAGCAGGAC CCCGTTTCAG GCCTGAGTGG CCGGAGTGGA
    TACCCTTCGA TGAGTCCACT CTCTCCCCAG GATATATTCC AGTTGGCTTG TATGGACCCC
    GCCGATGACG GACAGTTCCA AGAACGGCAG TCTCTGGTGG TGACAGACCC TAGCTCCATG
    AGACGTTCAT TTTCCACTAT TCGGGATAAG CGTTCAAATT CCTCGTGGTT GGAGGAATTC
    TCCATGGAGC GAAGCAGTGA AAATACCTAC AAGTCCCGTC GCCGGAGTTA CCACTCCTCC
    TTGCGGCTGT CAGCCCACCG CCTGAACTCT GATTCAGGCC ACAAGTCTGA CACTCACCGC
    TCAGGGGGCA GGGAGCGGGG ACGATCAAAA GAGCGAAAGC ATCTTCTCTC TCCTGATGTC
    TCCCGCTGCA ATTCAGAAGA GCGAGGGACC CAGGCTGACT GGGAGTCCCC AGAGCGCCGT
    CAATCCAGGT CACCCAGTGA GGGCAGGTCA CAGACGCCCA ACAGACAGGG CACAGGTTCC
    CTAAGTGAGA GCTCCATCCC CTCTGTCTCT GACACCAGCA CCCCAAGAAG AAGTCGTCGG
    CAGCTCCCAC CCGTCCCGCC AAAGCCCCGG CCCCTCCTTT CCTACAGCTC CCTGATTCGA
    CACGCGGGCA GCATCTCTCC ACCTGCTGAT GGAAGCGAGG AGGGCTCCCC GCTGACCTCC
    CAAGCTCTGG AGAGCAACAA TGCTTGCCTG ACCGAGTCTT CCAACTCTCC GCACCCCCAG
    CAGAGCCAAC ATGCCTCCCC ACAGCGCTAC ATCTCCGAGC CCTACTTGGC CCTGCACGAA
    GACTCCCACG CCTCAGACTG TGGTGAGGAG GAGACGCTCA CTTTCGAAGC AGCCGTGGCT
    ACTAGCCTGG GCCGTTCCAA CACCATCGGC TCAGCCCCAC CCCTGCGGCA TAGCTGGCAG
    ATGCCCAACG GGCACTATCG GCGGCGGAGG CGCGGGGGGC CTGGGCCAGG CATGATGTGT
    GGGGCTGTCA ACAACCTGCT AAGTGACACG GAAGAAGATG ACAAATGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.