cacna1e cDNA ORF clone, Danio rerio(zebrafish)

The following cacna1e gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1e cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa44276 XM_021478385.1
Latest version!
Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa44277 XM_017357425.2
Latest version!
Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa44278 XM_021478386.1
Latest version!
Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa44279 XM_017357427.2
Latest version!
Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa44280 XM_021478387.1
Latest version!
Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa44281 XM_021478388.1
Latest version!
Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
ODa44282 XM_017357424.2
Latest version!
Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa60028 XM_017357424.1
Latest version!
Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa60029 XM_017357425.1
Latest version!
Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa60030 XM_017357426.1
Latest version!
Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa60031 XM_017357427.1
Latest version!
Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
ODa60032 XM_017357428.1
Latest version!
Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa44276
    Clone ID Related Accession (Same CDS sequence) XM_021478385.1
    Accession Version XM_021478385.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7335bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTGGAAT TGGGCACTTG TTCGGTCAGC GCTCAGACTC TCTCTGACCG TAATGCCCTG 
    GCGGTCCCGT CATTTGGTCA GGGCATATGT GGCGGGAGCC CACCTTCACG ATGTTTCCTT
    CTCCAGCCGC AGGACCCGGA GAGCTGGCTC AAGCACCGGG GAACCCTGGA GGATGGCTCC
    CGCTCCTGTT CACTCCTGGA GCCTCAGAGC CTGGCTGACA GCCTTCTGTC CCATGCATCC
    AGTTTTGATA TGCTCTACGT CCCACATGGG CGTCCAATGG GCCTTGCACC GTCTTCTCTG
    GATCTGGGCG GCTCGTGTGA GGGGGCGGCC GCGTTGGGTC TGGACCCCGA CATGCCCGTC
    AGCCCGAATA TCATTAAACG GCGCAGGGGG GGACTCATTG AACAACGGGA CATCATTAAA
    GCACACCAGG CACACAAGAT CCAGAGCACC CCACAGGCCC GACGCAAGGA ATGGGAAATG
    GCTCGCTTCG GAGACGATGT TCCCGGTCTG CTGGGAGCCG GGGATGGGGG TTCGGAGCGC
    AGTCGGAACC GGGATGCTCC GGCGGTATCC ACAGGTGGAG TTTCACCAGC CTTCAAACAG
    ACCAAAGCGC AACGAGCCCG AACCATGGCT CTGTACAACC CCATTCCTGT CCGACAGAAC
    TGCTTCACCG TCAACAGATC TCTCTTCATC TTTGGAGAGG ACAACATCGT TAGGAAGTAT
    GCCAAGAAGA TCATAGAGTG GCCTCCGTTT GAATACATGA TCCTGGCCAC CATTATTGCT
    AACTGCATCG TCCTAGCCTT GGAGCAGCAC CTGCCTGGAG AAGACAAGAC ACCCATGGCT
    AAGAGACTTG AAAAAACAGA GCCCTACTTC ATAGGAATGT TTTGCTTTGA AGCTGGAATA
    AAAATCATTG CTTTGGGATT TGTATTCCAC AAGGGCTCGT ACCTGAGGAA TGGTTGGAAC
    GTCATGGACT TTATTGTCGT ATCGAGTGGG ATTCTGGCCA CAGCAGGAGC CCACATGAAT
    ATTTCATTAG ACCTGCGCAC ACTGAGGGCT GTGAGGGTAT TACGGCCCCT CAAACTGGTC
    TCTGGCATTC CTAGTCTGCA AATAGTTCTT AAATCCATAA TGAAGGCAAT GGTGCCATTG
    CTTCAGATCG GACTGCTACT GTTTTTTGCT ATTCTAATGT TTGCCATTAT TGGATTAGAG
    TTCTACAGTG GGAAACTCCA CAAGCAATGT GAACCTGTAG GTAAAGATTC TGAGAGAGCA
    GACTCGCCTG AGAATGAATT TCCATGTGGA AGCAAACTGT GTCCTGACCA TTATAACTGC
    TCTGGCTACT GGACGGGACC CAATGATGGC ATCACTCAGT TTGATAACAT CTTATTTTCT
    TTACTCACCG TCTTCCAGTG CATCACCATG GAAGGCTGGA CGACCATCCT CTATTACACC
    AACGATGCCT TAGGCTCTAC TTGGAATTGG CTCTACTTCA TTCCTCTAAT CATAATTGGC
    TCCTTCTTTG TGTTGAATCT GGTATTAGGA GTCCTGTCAG GGGAGTTTGC TAAAGAGCGA
    GAGCGGGTAG AGAACCGGAG AGCCTTCATG AAGCTTAGGA GACAGCAGCA GATTGAGAGG
    GAGCTGAACG GATATCGGGC CTGGATCGAC CGTGCAGAGG AGGTTATGCT TGCAGAGGAG
    AACAAGAATT CAGGACCTTC TGCTTTGGAT GTGCTAAAAA GAGCTACCAC CATAAAGAGA
    AGAGGAGTGG ATGATGCTCG AAGAGCTCCA GAAGAGCAGT ATGCTGACAT CTCATCTCTA
    GGGATGCCAC TGAACAGAGC CAGCATACGC AGTGCTAAAA GAGGACCAAC ATCCTATTTC
    CGACGGAAAG AGCGCCTGCT ACGGATCTCC GTCCGCCGTA TGGTGAAGAC TCAGACGTTC
    TACTGGACTG TTCTGGGTCT GGTAGCCCTC AACACTCTCT GTGTAGCCAT CGTACATCAC
    AAACAGCCAC AGTGGTTATC TAGTCTCCTC TACTATGCAG AGTTTTTGTT TCTGGGTTTG
    TTTCTTACCG AGATGTTTCT GAAGATGTAT GGACTCGGAC CCAGACTCTA TTTTCACTCC
    TCCTTCAACT GCTTCGATTG TGGGGTTATA GTAGGCAGCA TCTTTGAGGT GTTCTGGGGC
    TTCTTCAGAC CTGGCACATC CTTTGGGATC AGCGTTCTTC GTGCCTTGCG GTTGTTGAGA
    ATTTTCAAGA TAACCAAATA CTGGGCGTCC CTGAGGAACC TGGTCGTGTC TCTGATGAAC
    TCAATGAAGT CTATTATCAG TTTGCTCTTC CTGCTTTTTC TCTTTATTGT GGTCTTTGCA
    CTTCTGGGCA TGCAGCTCTT TGGTGGAAGG TTCATTTTTG ATGAGTACAC TCCCACCAAT
    TTTGACACTT TCCCAGCAGC CATCATGACA GTGTTTCAAA TTCTGACTGG TGAGGACTGG
    AATGAAGTCA TGTACAATGG CATCCGGTCT CAAGGTGGCG TGAAATCTGG GATGTGGTCG
    TCTGTCTACT TCATTGTTCT CACTCTGTTT GGAAATTATA CGCTGCTTAA CGTCTTCTTG
    GCTATCGCTG TCGACAATTT GGCCAATGCC CAGGAGCTCA CAAAGGACGA AGAGGAGGAG
    GAAGAGGCGT TTAATCAGAA ACATGCCCTG CAGAAAGCCA AGGAGGTTGG GCCCATGTCC
    TCTTTCATGT CCTCAGAGAG GAGGAGGAGG CCCTACCTGT ACAGGAAGCG TGCAATTCAT
    CGGAGCCGTG CAGCCTACTC CTTCGAGGAT GCAGAGAGTC TGGCTGACGC TCGTCCATTC
    AATGCCTCTA ACTCTTTCAT GTCCAGGAGA GAGAGGAGGA GGAGGTTGAC CATGTCAGTG
    TGGGAGCAGA GGACCAGCCA GCTGCGGCGG CACAGGCAGA TGTCCAGCCG CGAGATCCTT
    TTCAGCAGCC CCAGTGAAGA ACAGGACGGA CCACCAGGCT GCCCTCTCCA CCGGAAACCT
    GGACTATCTC CATCTGGCAG CAAGCGCAGA GCCACTGAGA CCACTTCAAC CACAATCATG
    CCAGAGGGTA CCTTAGAGCC ACCCATGCCT ATGGACATGC CGGTGGATGA GCAAGCTCTG
    CCCATTTCTA TTCCTGAACC CCCTTTAGCC ATTCCAATTC CAGAGCCACC AATGCCCGTG
    GACGCATGCC TCCCTGAACC CCCAGTTACA CTGAATCTGC CCTTAACAGA GCCATCTGAA
    TCAAAGACTT CACCCGAGGG TGCTCTGGAC ATCAACAACC ATTGCCCTAG ACTAAATGGG
    AACAGGCGGC AGCGGGCTGT CCGTAAGCAC AGACCTCCTG CTATGGGGGA CATGCTGACC
    CCTGATATGG GGCCCAGGCC CCGACGGCCA CGCCACCGCG AGCTTCACAC AGACCCCAGG
    GGAAAAGAGA CTCTGCAAGG TGATGGTGTT GACGGTAAAG AACAAAAGCC AGGAAGTGAG
    GGTGATGAAA AGAGTGATGG AGAAATCAGT CCAGATGGCA GAGGGCTTGA ACAAAGGCAG
    AACAAACCAG ACAACACAGA ATCATCCACA GAGTCCAAAC CAGCAAATTC CCCCCGCAAA
    ATCCCACCTC TTGACTCTGA ACCTGCTACT CTACAGCTCA CTGACAACAC ACTGCAGCAG
    TCTTCACAGG GAACTACATG TGCCGGTGAG ACCGATAACA ATAACCACGG GTCCTTGAAT
    CAGGATAAGT CCTCCCTCAA CACCAGCATC GTGATAGAGG TCACCGACAC TCAGCCATCC
    ATGGAGCTGA CCTCCATTAC GGGTAATAGT AAAGATGTGG AGGCCTCAAA GCCAGAGAAA
    GAAGAAGAAA CCCCAGATGC ACCCAAAGGG CCTCCACCTG TCAGCATGTT CATCTTCAAA
    CCCAACAACC CGGTCCGCAG GGCCTGCCAC TACGTTGTCA ACCTTCGTTA TTTTGAGATG
    TCAATCCTTC TTGTGATCGC CGCCAGCAGT ATCGCATTGG CTGCCGAAGA CCCCGTCAAC
    ACTAATTCGG ACAGGAACAA AGTTCTGCGC TATTTTGACT ATGTATTCAC TGGGGTCTTC
    ACGTTTGAAA TGATAATCAA GATGATTGAT CAGGGGTTGG TCTTACACTA TGGCTCCTAT
    TTTCGTGACC CGTGGAACAT TCTGGATTTC ATAGTTGTGG TGGGAGCTCT GGTGGCTTTT
    GCTCTCACGA ATGTGATGGG GAGTAACACA GGAAGAGACA TTAAAACCAT TAAATCGCTG
    CGTGTACTCC GAGTTCTGAG GCCTCTCAAG ACGATTAAAA GACTTCCTAA ACTCAAGGCT
    GTGTTTGACT GTGTGGTAAC ATCACTGAAG AATGTTTTCA ACATCCTCAT CGTCTACAAG
    CTGTTTATGT TCATATTTGC CGTCATTGCC GTGCAGCTTT TCAAAGGGAA ATTTTACTAC
    TGTACCGACA GTTCCAAGGA CACTGAAAAC GAGTGCAAAG GCTACTACAT TGACTATGAT
    AAAGACAAGA AAAAGGAGAA ACGAGAGTGG AAGAGACGCG ATTTCCATTA TGATAACGTT
    GTCTGGGCTC TGTTGACTCT CTTCACCGTA TCCACTGGCG AAGGCTGGCC GCAAGTTCTG
    CAACACTCTG TGGATGTGAC AGAGGAGGAC CGGGGTCCCA GTCAGGGGAA CAGGATGGAG
    ATGTCTATCT TCTATGTCAT TTATTTTGTG GTGTTCCCCT TCTTCTTCGT CAACATATTT
    GTGGCCCTCA TCATCATCAC TTTCCAAGAA CAGGGTGACA AAATGATGGA GGAGTGTAGT
    CTGGAGAAAA ACGAGAGGGC TTGTATCGAC TTTGCCATCA GTGCCAAACC TTTGACTCGC
    TACATGCCCC AGAACAGACA GACCTTACAG TACAGACTGT GGCAATTTGT GGTGTCGCCC
    TCATTTGAGT ACACAGTACT GACAATGATC GCCCTTAACA CCATCGTTCT CATGATGAAG
    CATCATGATC CTCGTCTTGA AGGCTATGAC GATGTGCTCA AATATCTCAA CACAATTTTC
    ACCGTTATTT TCTCTGTTGA GTGTGTTCTC AAGATCCTGG CTTTTGGTAT AATGAACTAT
    TTCAGAGACA CATGGAATAT ATTTGACTTC ATCACGGTTC TAGGAAGCAT CACAGAAATT
    CTGGTGGACA AATTTCAGAC TGGAAAAACT TTTAATATGG GCTTCCTGAA GCTATTCCGG
    GCAGCTCGGC TGATTAAACT CCTGCGTCAG GGCTACACCA TCCGTATTCT GCTCTGGACT
    TTTGTTCAGT CCTTCAAGGC TCTACCATAT GTCTGTCTGC TCATCGCCAT GCTGTTTTTC
    ATCTATGCCA TCATTGGCAT GCAGGTATTT GGCAACATCA AGATAAATGA GAAAACCCAA
    ATCAATGAAC ACAATAACTT CAGGACTTTC TTTGGTGCAC TCATGCTTTT GTTCAGGAGT
    GCAACAGGTG AATCATGGCA GGAAATCATG CTATCGTGTT TAGGAGAGAA GGAGTGTGAA
    ATTGACACTT CTGTCAACTC AACAACAAAG ACAATAAAAA ACGACTGCGG TACCGACTTT
    GCCTACTTCT ACTTTGTCTC CTTCATCTTC TTCAGTTCTT TTCTGATGCT GAATCTGTTT
    GTGGCTGTGA TCATGGATAA TTTTGAGTAC CTGACACGTG ACTCATCCAT TCTGGGTCCT
    CACCACTTAG ATGAGTTTGT ACGGATCTGG GGAGAGTATG ACCGTGCTGC ATGTGGCCGT
    ATTCATTACA CTGATATGTA CGAGATGCTG ACCTGCATGT CGCCACCGCT TGGCCTGGGC
    AAGAAATGTC CCTCCAAAGT GGCGTATAAG CGGTTGGTTC TGATGAACAT GCCAGTGGAT
    GAAGACATGT CTGTCCACTT CACCTCCACA CTTATGGCCC TCATACGCAC CGCACTGGAC
    ATTAAAATTG CTCGAGGAGG GGAAGACAGG GGTCAGATGG ATGTTGAACT GCAGAAGGAA
    ATCAGTGTCA TCTGGCCGCA TCTCTCACAG AAATCTCTTG ATCTGCTTGT ACCCATTCAC
    AAAGCTAGTG ACATGACCAT TGGGAAGATC TATGCAGCCA TGATGATCAT GGACTACTAC
    AAGCAGAACA AAGCCAAGAA GCTCCGTCAG CAGCTCGAGG AACAGAAACA CGCACCAATG
    TTCCAACGCA TGGATGCATC CTCGCTGCCG CAGGACATCC TCTGCAGTGC CAAGGCTTTG
    CCATTCCTGA CTCACAGTGC AGGATCTGCC CTCACTAGAG GTGGGTTTGT GGCACTGAGT
    CCTATCTCTC CTCAAGATCT CTTCATGCAG CCCATGACCC AAGACAGCAT GGAGGAGAGG
    GAGGAATACC AACACCCGTA TCACCTGCAA AGCATGGTGC CTGAGCACAA AGAATTTAGC
    AGTGTGATGT GCCCCACTAG CATGCAGGCT GATCCACTGC AAGTGCCAGG AAGAGGCGTC
    TCTATCCGAG CCTCTTCCAT GCCTCGCCTG GCGGTAGAGC CACAGGCCTC TTTAGAGAAC
    AAACTGATGA AGCGCTCATT CTCCACCATC GGGGATCAGT GTGTAAATGG TGACTGGCAG
    GAAGAGTTCT CTCTGGAGAG GGTGAGTCCT GACCGGCTGG AGAGACCCAG GCAAAGAAGC
    TTCAGAGGTG TCAGGTCAAA CCTGCGGGAG CCAGTGGACA AAGAGAGAGG ACGGTCTAAA
    GAACGAAGAC ACCTGCTGTC TCCAGATGTG TCTCGCTGCA ACTCAGAGGA AAGGAGTCAG
    TCTCAGGAGA GACGTCATTC TCCATCGCCC AGTGAGGGGA GGAAGCACAC AGCTCTCAGA
    CAGGGAAATA ATACAGGCAG CCCTATTGCC TCCACGTCTG ATTCAGGGAC GCCTCGAGGA
    CGCCGCCAAC TCCCACAGAC ACCCTCTCGC CCTCGGCCTC ATATCTCCTA CTCTCCTTTA
    GTGTGCCGTG CCCATCCAGC ACCAGGGGAT GATCGGCCAC GAGCTCCACC CGCAGAGGAA
    GGAGAGGAGC CCACTGCAAC CGGCCCAGGC ACCGCCATGG CCCACTCTAC CCCTCACCGC
    TACATCTCAG AGCCCTACCT GCCACTGCAG GAGGACGTCA ACAGTCCAGA CTCCAGTGGT
    GTGGAGGAGA CTCTCACTTT TGAGGCTGCA ATAGCAACCA GCCTGGGCCG CTCTAATACC
    ATTAGCTCGG CGCCACAGCT ACGTCACTCC TGGCAGGTGC CTAATGGACA TTTCCGCAAA
    CGACTAGCAC AAGCCACAGC TGGGACGTCC GGCTGTGAGC TTCTCAGTGA CACCGATGAG
    GATGACCGCT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334060.1
    CDS388..7722
    Translation

    Target ORF information:

    RefSeq Version XM_021478385.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478385.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTGGAAT TGGGCACTTG TTCGGTCAGC GCTCAGACTC TCTCTGACCG TAATGCCCTG 
    GCGGTCCCGT CATTTGGTCA GGGCATATGT GGCGGGAGCC CACCTTCACG ATGTTTCCTT
    CTCCAGCCGC AGGACCCGGA GAGCTGGCTC AAGCACCGGG GAACCCTGGA GGATGGCTCC
    CGCTCCTGTT CACTCCTGGA GCCTCAGAGC CTGGCTGACA GCCTTCTGTC CCATGCATCC
    AGTTTTGATA TGCTCTACGT CCCACATGGG CGTCCAATGG GCCTTGCACC GTCTTCTCTG
    GATCTGGGCG GCTCGTGTGA GGGGGCGGCC GCGTTGGGTC TGGACCCCGA CATGCCCGTC
    AGCCCGAATA TCATTAAACG GCGCAGGGGG GGACTCATTG AACAACGGGA CATCATTAAA
    GCACACCAGG CACACAAGAT CCAGAGCACC CCACAGGCCC GACGCAAGGA ATGGGAAATG
    GCTCGCTTCG GAGACGATGT TCCCGGTCTG CTGGGAGCCG GGGATGGGGG TTCGGAGCGC
    AGTCGGAACC GGGATGCTCC GGCGGTATCC ACAGGTGGAG TTTCACCAGC CTTCAAACAG
    ACCAAAGCGC AACGAGCCCG AACCATGGCT CTGTACAACC CCATTCCTGT CCGACAGAAC
    TGCTTCACCG TCAACAGATC TCTCTTCATC TTTGGAGAGG ACAACATCGT TAGGAAGTAT
    GCCAAGAAGA TCATAGAGTG GCCTCCGTTT GAATACATGA TCCTGGCCAC CATTATTGCT
    AACTGCATCG TCCTAGCCTT GGAGCAGCAC CTGCCTGGAG AAGACAAGAC ACCCATGGCT
    AAGAGACTTG AAAAAACAGA GCCCTACTTC ATAGGAATGT TTTGCTTTGA AGCTGGAATA
    AAAATCATTG CTTTGGGATT TGTATTCCAC AAGGGCTCGT ACCTGAGGAA TGGTTGGAAC
    GTCATGGACT TTATTGTCGT ATCGAGTGGG ATTCTGGCCA CAGCAGGAGC CCACATGAAT
    ATTTCATTAG ACCTGCGCAC ACTGAGGGCT GTGAGGGTAT TACGGCCCCT CAAACTGGTC
    TCTGGCATTC CTAGTCTGCA AATAGTTCTT AAATCCATAA TGAAGGCAAT GGTGCCATTG
    CTTCAGATCG GACTGCTACT GTTTTTTGCT ATTCTAATGT TTGCCATTAT TGGATTAGAG
    TTCTACAGTG GGAAACTCCA CAAGCAATGT GAACCTGTAG GTAAAGATTC TGAGAGAGCA
    GACTCGCCTG AGAATGAATT TCCATGTGGA AGCAAACTGT GTCCTGACCA TTATAACTGC
    TCTGGCTACT GGACGGGACC CAATGATGGC ATCACTCAGT TTGATAACAT CTTATTTTCT
    TTACTCACCG TCTTCCAGTG CATCACCATG GAAGGCTGGA CGACCATCCT CTATTACACC
    AACGATGCCT TAGGCTCTAC TTGGAATTGG CTCTACTTCA TTCCTCTAAT CATAATTGGC
    TCCTTCTTTG TGTTGAATCT GGTATTAGGA GTCCTGTCAG GGGAGTTTGC TAAAGAGCGA
    GAGCGGGTAG AGAACCGGAG AGCCTTCATG AAGCTTAGGA GACAGCAGCA GATTGAGAGG
    GAGCTGAACG GATATCGGGC CTGGATCGAC CGTGCAGAGG AGGTTATGCT TGCAGAGGAG
    AACAAGAATT CAGGACCTTC TGCTTTGGAT GTGCTAAAAA GAGCTACCAC CATAAAGAGA
    AGAGGAGTGG ATGATGCTCG AAGAGCTCCA GAAGAGCAGT ATGCTGACAT CTCATCTCTA
    GGGATGCCAC TGAACAGAGC CAGCATACGC AGTGCTAAAA GAGGACCAAC ATCCTATTTC
    CGACGGAAAG AGCGCCTGCT ACGGATCTCC GTCCGCCGTA TGGTGAAGAC TCAGACGTTC
    TACTGGACTG TTCTGGGTCT GGTAGCCCTC AACACTCTCT GTGTAGCCAT CGTACATCAC
    AAACAGCCAC AGTGGTTATC TAGTCTCCTC TACTATGCAG AGTTTTTGTT TCTGGGTTTG
    TTTCTTACCG AGATGTTTCT GAAGATGTAT GGACTCGGAC CCAGACTCTA TTTTCACTCC
    TCCTTCAACT GCTTCGATTG TGGGGTTATA GTAGGCAGCA TCTTTGAGGT GTTCTGGGGC
    TTCTTCAGAC CTGGCACATC CTTTGGGATC AGCGTTCTTC GTGCCTTGCG GTTGTTGAGA
    ATTTTCAAGA TAACCAAATA CTGGGCGTCC CTGAGGAACC TGGTCGTGTC TCTGATGAAC
    TCAATGAAGT CTATTATCAG TTTGCTCTTC CTGCTTTTTC TCTTTATTGT GGTCTTTGCA
    CTTCTGGGCA TGCAGCTCTT TGGTGGAAGG TTCATTTTTG ATGAGTACAC TCCCACCAAT
    TTTGACACTT TCCCAGCAGC CATCATGACA GTGTTTCAAA TTCTGACTGG TGAGGACTGG
    AATGAAGTCA TGTACAATGG CATCCGGTCT CAAGGTGGCG TGAAATCTGG GATGTGGTCG
    TCTGTCTACT TCATTGTTCT CACTCTGTTT GGAAATTATA CGCTGCTTAA CGTCTTCTTG
    GCTATCGCTG TCGACAATTT GGCCAATGCC CAGGAGCTCA CAAAGGACGA AGAGGAGGAG
    GAAGAGGCGT TTAATCAGAA ACATGCCCTG CAGAAAGCCA AGGAGGTTGG GCCCATGTCC
    TCTTTCATGT CCTCAGAGAG GAGGAGGAGG CCCTACCTGT ACAGGAAGCG TGCAATTCAT
    CGGAGCCGTG CAGCCTACTC CTTCGAGGAT GCAGAGAGTC TGGCTGACGC TCGTCCATTC
    AATGCCTCTA ACTCTTTCAT GTCCAGGAGA GAGAGGAGGA GGAGGTTGAC CATGTCAGTG
    TGGGAGCAGA GGACCAGCCA GCTGCGGCGG CACAGGCAGA TGTCCAGCCG CGAGATCCTT
    TTCAGCAGCC CCAGTGAAGA ACAGGACGGA CCACCAGGCT GCCCTCTCCA CCGGAAACCT
    GGACTATCTC CATCTGGCAG CAAGCGCAGA GCCACTGAGA CCACTTCAAC CACAATCATG
    CCAGAGGGTA CCTTAGAGCC ACCCATGCCT ATGGACATGC CGGTGGATGA GCAAGCTCTG
    CCCATTTCTA TTCCTGAACC CCCTTTAGCC ATTCCAATTC CAGAGCCACC AATGCCCGTG
    GACGCATGCC TCCCTGAACC CCCAGTTACA CTGAATCTGC CCTTAACAGA GCCATCTGAA
    TCAAAGACTT CACCCGAGGG TGCTCTGGAC ATCAACAACC ATTGCCCTAG ACTAAATGGG
    AACAGGCGGC AGCGGGCTGT CCGTAAGCAC AGACCTCCTG CTATGGGGGA CATGCTGACC
    CCTGATATGG GGCCCAGGCC CCGACGGCCA CGCCACCGCG AGCTTCACAC AGACCCCAGG
    GGAAAAGAGA CTCTGCAAGG TGATGGTGTT GACGGTAAAG AACAAAAGCC AGGAAGTGAG
    GGTGATGAAA AGAGTGATGG AGAAATCAGT CCAGATGGCA GAGGGCTTGA ACAAAGGCAG
    AACAAACCAG ACAACACAGA ATCATCCACA GAGTCCAAAC CAGCAAATTC CCCCCGCAAA
    ATCCCACCTC TTGACTCTGA ACCTGCTACT CTACAGCTCA CTGACAACAC ACTGCAGCAG
    TCTTCACAGG GAACTACATG TGCCGGTGAG ACCGATAACA ATAACCACGG GTCCTTGAAT
    CAGGATAAGT CCTCCCTCAA CACCAGCATC GTGATAGAGG TCACCGACAC TCAGCCATCC
    ATGGAGCTGA CCTCCATTAC GGGTAATAGT AAAGATGTGG AGGCCTCAAA GCCAGAGAAA
    GAAGAAGAAA CCCCAGATGC ACCCAAAGGG CCTCCACCTG TCAGCATGTT CATCTTCAAA
    CCCAACAACC CGGTCCGCAG GGCCTGCCAC TACGTTGTCA ACCTTCGTTA TTTTGAGATG
    TCAATCCTTC TTGTGATCGC CGCCAGCAGT ATCGCATTGG CTGCCGAAGA CCCCGTCAAC
    ACTAATTCGG ACAGGAACAA AGTTCTGCGC TATTTTGACT ATGTATTCAC TGGGGTCTTC
    ACGTTTGAAA TGATAATCAA GATGATTGAT CAGGGGTTGG TCTTACACTA TGGCTCCTAT
    TTTCGTGACC CGTGGAACAT TCTGGATTTC ATAGTTGTGG TGGGAGCTCT GGTGGCTTTT
    GCTCTCACGA ATGTGATGGG GAGTAACACA GGAAGAGACA TTAAAACCAT TAAATCGCTG
    CGTGTACTCC GAGTTCTGAG GCCTCTCAAG ACGATTAAAA GACTTCCTAA ACTCAAGGCT
    GTGTTTGACT GTGTGGTAAC ATCACTGAAG AATGTTTTCA ACATCCTCAT CGTCTACAAG
    CTGTTTATGT TCATATTTGC CGTCATTGCC GTGCAGCTTT TCAAAGGGAA ATTTTACTAC
    TGTACCGACA GTTCCAAGGA CACTGAAAAC GAGTGCAAAG GCTACTACAT TGACTATGAT
    AAAGACAAGA AAAAGGAGAA ACGAGAGTGG AAGAGACGCG ATTTCCATTA TGATAACGTT
    GTCTGGGCTC TGTTGACTCT CTTCACCGTA TCCACTGGCG AAGGCTGGCC GCAAGTTCTG
    CAACACTCTG TGGATGTGAC AGAGGAGGAC CGGGGTCCCA GTCAGGGGAA CAGGATGGAG
    ATGTCTATCT TCTATGTCAT TTATTTTGTG GTGTTCCCCT TCTTCTTCGT CAACATATTT
    GTGGCCCTCA TCATCATCAC TTTCCAAGAA CAGGGTGACA AAATGATGGA GGAGTGTAGT
    CTGGAGAAAA ACGAGAGGGC TTGTATCGAC TTTGCCATCA GTGCCAAACC TTTGACTCGC
    TACATGCCCC AGAACAGACA GACCTTACAG TACAGACTGT GGCAATTTGT GGTGTCGCCC
    TCATTTGAGT ACACAGTACT GACAATGATC GCCCTTAACA CCATCGTTCT CATGATGAAG
    CATCATGATC CTCGTCTTGA AGGCTATGAC GATGTGCTCA AATATCTCAA CACAATTTTC
    ACCGTTATTT TCTCTGTTGA GTGTGTTCTC AAGATCCTGG CTTTTGGTAT AATGAACTAT
    TTCAGAGACA CATGGAATAT ATTTGACTTC ATCACGGTTC TAGGAAGCAT CACAGAAATT
    CTGGTGGACA AATTTCAGAC TGGAAAAACT TTTAATATGG GCTTCCTGAA GCTATTCCGG
    GCAGCTCGGC TGATTAAACT CCTGCGTCAG GGCTACACCA TCCGTATTCT GCTCTGGACT
    TTTGTTCAGT CCTTCAAGGC TCTACCATAT GTCTGTCTGC TCATCGCCAT GCTGTTTTTC
    ATCTATGCCA TCATTGGCAT GCAGGTATTT GGCAACATCA AGATAAATGA GAAAACCCAA
    ATCAATGAAC ACAATAACTT CAGGACTTTC TTTGGTGCAC TCATGCTTTT GTTCAGGAGT
    GCAACAGGTG AATCATGGCA GGAAATCATG CTATCGTGTT TAGGAGAGAA GGAGTGTGAA
    ATTGACACTT CTGTCAACTC AACAACAAAG ACAATAAAAA ACGACTGCGG TACCGACTTT
    GCCTACTTCT ACTTTGTCTC CTTCATCTTC TTCAGTTCTT TTCTGATGCT GAATCTGTTT
    GTGGCTGTGA TCATGGATAA TTTTGAGTAC CTGACACGTG ACTCATCCAT TCTGGGTCCT
    CACCACTTAG ATGAGTTTGT ACGGATCTGG GGAGAGTATG ACCGTGCTGC ATGTGGCCGT
    ATTCATTACA CTGATATGTA CGAGATGCTG ACCTGCATGT CGCCACCGCT TGGCCTGGGC
    AAGAAATGTC CCTCCAAAGT GGCGTATAAG CGGTTGGTTC TGATGAACAT GCCAGTGGAT
    GAAGACATGT CTGTCCACTT CACCTCCACA CTTATGGCCC TCATACGCAC CGCACTGGAC
    ATTAAAATTG CTCGAGGAGG GGAAGACAGG GGTCAGATGG ATGTTGAACT GCAGAAGGAA
    ATCAGTGTCA TCTGGCCGCA TCTCTCACAG AAATCTCTTG ATCTGCTTGT ACCCATTCAC
    AAAGCTAGTG ACATGACCAT TGGGAAGATC TATGCAGCCA TGATGATCAT GGACTACTAC
    AAGCAGAACA AAGCCAAGAA GCTCCGTCAG CAGCTCGAGG AACAGAAACA CGCACCAATG
    TTCCAACGCA TGGATGCATC CTCGCTGCCG CAGGACATCC TCTGCAGTGC CAAGGCTTTG
    CCATTCCTGA CTCACAGTGC AGGATCTGCC CTCACTAGAG GTGGGTTTGT GGCACTGAGT
    CCTATCTCTC CTCAAGATCT CTTCATGCAG CCCATGACCC AAGACAGCAT GGAGGAGAGG
    GAGGAATACC AACACCCGTA TCACCTGCAA AGCATGGTGC CTGAGCACAA AGAATTTAGC
    AGTGTGATGT GCCCCACTAG CATGCAGGCT GATCCACTGC AAGTGCCAGG AAGAGGCGTC
    TCTATCCGAG CCTCTTCCAT GCCTCGCCTG GCGGTAGAGC CACAGGCCTC TTTAGAGAAC
    AAACTGATGA AGCGCTCATT CTCCACCATC GGGGATCAGT GTGTAAATGG TGACTGGCAG
    GAAGAGTTCT CTCTGGAGAG GGTGAGTCCT GACCGGCTGG AGAGACCCAG GCAAAGAAGC
    TTCAGAGGTG TCAGGTCAAA CCTGCGGGAG CCAGTGGACA AAGAGAGAGG ACGGTCTAAA
    GAACGAAGAC ACCTGCTGTC TCCAGATGTG TCTCGCTGCA ACTCAGAGGA AAGGAGTCAG
    TCTCAGGAGA GACGTCATTC TCCATCGCCC AGTGAGGGGA GGAAGCACAC AGCTCTCAGA
    CAGGGAAATA ATACAGGCAG CCCTATTGCC TCCACGTCTG ATTCAGGGAC GCCTCGAGGA
    CGCCGCCAAC TCCCACAGAC ACCCTCTCGC CCTCGGCCTC ATATCTCCTA CTCTCCTTTA
    GTGTGCCGTG CCCATCCAGC ACCAGGGGAT GATCGGCCAC GAGCTCCACC CGCAGAGGAA
    GGAGAGGAGC CCACTGCAAC CGGCCCAGGC ACCGCCATGG CCCACTCTAC CCCTCACCGC
    TACATCTCAG AGCCCTACCT GCCACTGCAG GAGGACGTCA ACAGTCCAGA CTCCAGTGGT
    GTGGAGGAGA CTCTCACTTT TGAGGCTGCA ATAGCAACCA GCCTGGGCCG CTCTAATACC
    ATTAGCTCGG CGCCACAGCT ACGTCACTCC TGGCAGGTGC CTAATGGACA TTTCCGCAAA
    CGACTAGCAC AAGCCACAGC TGGGACGTCC GGCTGTGAGC TTCTCAGTGA CACCGATGAG
    GATGACCGCT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44277
    Clone ID Related Accession (Same CDS sequence) XM_017357425.2
    Accession Version XM_017357425.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7335bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTGGAAT TGGGCACTTG TTCGGTCAGC GCTCAGACTC TCTCTGACCG TAATGCCCTG 
    GCGGTCCCGT CATTTGGTCA GGGCATATGT GGCGGGAGCC CACCTTCACG ATGTTTCCTT
    CTCCAGCCGC AGGACCCGGA GAGCTGGCTC AAGCACCGGG GAACCCTGGA GGATGGCTCC
    CGCTCCTGTT CACTCCTGGA GCCTCAGAGC CTGGCTGACA GCCTTCTGTC CCATGCATCC
    AGTTTTGATA TGCTCTACGT CCCACATGGG CGTCCAATGG GCCTTGCACC GTCTTCTCTG
    GATCTGGGCG GCTCGTGTGA GGGGGCGGCC GCGTTGGGTC TGGACCCCGA CATGCCCGTC
    AGCCCGAATA TCATTAAACG GCGCAGGGGG GGACTCATTG AACAACGGGA CATCATTAAA
    GCACACCAGG CACACAAGAT CCAGAGCACC CCACAGGCCC GACGCAAGGA ATGGGAAATG
    GCTCGCTTCG GAGACGATGT TCCCGGTCTG CTGGGAGCCG GGGATGGGGG TTCGGAGCGC
    AGTCGGAACC GGGATGCTCC GGCGGTATCC ACAGGTGGAG TTTCACCAGC CTTCAAACAG
    ACCAAAGCGC AACGAGCCCG AACCATGGCT CTGTACAACC CCATTCCTGT CCGACAGAAC
    TGCTTCACCG TCAACAGATC TCTCTTCATC TTTGGAGAGG ACAACATCGT TAGGAAGTAT
    GCCAAGAAGA TCATAGAGTG GCCTCCGTTT GAATACATGA TCCTGGCCAC CATTATTGCT
    AACTGCATCG TCCTAGCCTT GGAGCAGCAC CTGCCTGGAG AAGACAAGAC ACCCATGGCT
    AAGAGACTTG AAAAAACAGA GCCCTACTTC ATAGGAATGT TTTGCTTTGA AGCTGGAATA
    AAAATCATTG CTTTGGGATT TGTATTCCAC AAGGGCTCGT ACCTGAGGAA TGGTTGGAAC
    GTCATGGACT TTATTGTCGT ATCGAGTGGG ATTCTGGCCA CAGCAGGAGC CCACATGAAT
    ATTTCATTAG ACCTGCGCAC ACTGAGGGCT GTGAGGGTAT TACGGCCCCT CAAACTGGTC
    TCTGGCATTC CTAGTCTGCA AATAGTTCTT AAATCCATAA TGAAGGCAAT GGTGCCATTG
    CTTCAGATCG GACTGCTACT GTTTTTTGCT ATTCTAATGT TTGCCATTAT TGGATTAGAG
    TTCTACAGTG GGAAACTCCA CAAGCAATGT GAACCTGTAG GTAAAGATTC TGAGAGAGCA
    GACTCGCCTG AGAATGAATT TCCATGTGGA AGCAAACTGT GTCCTGACCA TTATAACTGC
    TCTGGCTACT GGACGGGACC CAATGATGGC ATCACTCAGT TTGATAACAT CTTATTTTCT
    TTACTCACCG TCTTCCAGTG CATCACCATG GAAGGCTGGA CGACCATCCT CTATTACACC
    AACGATGCCT TAGGCTCTAC TTGGAATTGG CTCTACTTCA TTCCTCTAAT CATAATTGGC
    TCCTTCTTTG TGTTGAATCT GGTATTAGGA GTCCTGTCAG GGGAGTTTGC TAAAGAGCGA
    GAGCGGGTAG AGAACCGGAG AGCCTTCATG AAGCTTAGGA GACAGCAGCA GATTGAGAGG
    GAGCTGAACG GATATCGGGC CTGGATCGAC CGTGCAGAGG AGGTTATGCT TGCAGAGGAG
    AACAAGAATT CAGGACCTTC TGCTTTGGAT GTGCTAAAAA GAGCTACCAC CATAAAGAGA
    AGAGGAGTGG ATGATGCTCG AAGAGCTCCA GAAGAGCAGT ATGCTGACAT CTCATCTCTA
    GGGATGCCAC TGAACAGAGC CAGCATACGC AGTGCTAAAA GAGGACCAAC ATCCTATTTC
    CGACGGAAAG AGCGCCTGCT ACGGATCTCC GTCCGCCGTA TGGTGAAGAC TCAGACGTTC
    TACTGGACTG TTCTGGGTCT GGTAGCCCTC AACACTCTCT GTGTAGCCAT CGTACATCAC
    AAACAGCCAC AGTGGTTATC TAGTCTCCTC TACTATGCAG AGTTTTTGTT TCTGGGTTTG
    TTTCTTACCG AGATGTTTCT GAAGATGTAT GGACTCGGAC CCAGACTCTA TTTTCACTCC
    TCCTTCAACT GCTTCGATTG TGGGGTTATA GTAGGCAGCA TCTTTGAGGT GTTCTGGGGC
    TTCTTCAGAC CTGGCACATC CTTTGGGATC AGCGTTCTTC GTGCCTTGCG GTTGTTGAGA
    ATTTTCAAGA TAACCAAATA CTGGGCGTCC CTGAGGAACC TGGTCGTGTC TCTGATGAAC
    TCAATGAAGT CTATTATCAG TTTGCTCTTC CTGCTTTTTC TCTTTATTGT GGTCTTTGCA
    CTTCTGGGCA TGCAGCTCTT TGGTGGAAGG TTCATTTTTG ATGAGTACAC TCCCACCAAT
    TTTGACACTT TCCCAGCAGC CATCATGACA GTGTTTCAAA TTCTGACTGG TGAGGACTGG
    AATGAAGTCA TGTACAATGG CATCCGGTCT CAAGGTGGCG TGAAATCTGG GATGTGGTCG
    TCTGTCTACT TCATTGTTCT CACTCTGTTT GGAAATTATA CGCTGCTTAA CGTCTTCTTG
    GCTATCGCTG TCGACAATTT GGCCAATGCC CAGGAGCTCA CAAAGGACGA AGAGGAGGAG
    GAAGAGGCGT TTAATCAGAA ACATGCCCTG CAGAAAGCCA AGGAGGTTGG GCCCATGTCC
    TCTTTCATGT CCTCAGAGAG GAGGAGGAGG CCCTACCTGT ACAGGAAGCG TGCAATTCAT
    CGGAGCCGTG CAGCCTACTC CTTCGAGGAT GCAGAGAGTC TGGCTGACGC TCGTCCATTC
    AATGCCTCTA ACTCTTTCAT GTCCAGGAGA GAGAGGAGGA GGAGGTTGAC CATGTCAGTG
    TGGGAGCAGA GGACCAGCCA GCTGCGGCGG CACAGGCAGA TGTCCAGCCG CGAGATCCTT
    TTCAGCAGCC CCAGTGAAGA ACAGGACGGA CCACCAGGCT GCCCTCTCCA CCGGAAACCT
    GGACTATCTC CATCTGGCAG CAAGCGCAGA GCCACTGAGA CCACTTCAAC CACAATCATG
    CCAGAGGGTA CCTTAGAGCC ACCCATGCCT ATGGACATGC CGGTGGATGA GCAAGCTCTG
    CCCATTTCTA TTCCTGAACC CCCTTTAGCC ATTCCAATTC CAGAGCCACC AATGCCCGTG
    GACGCATGCC TCCCTGAACC CCCAGTTACA CTGAATCTGC CCTTAACAGA GCCATCTGAA
    TCAAAGACTT CACCCGAGGG TGCTCTGGAC ATCAACAACC ATTGCCCTAG ACTAAATGGG
    AACAGGCGGC AGCGGGCTGT CCGTAAGCAC AGACCTCCTG CTATGGGGGA CATGCTGACC
    CCTGATATGG GGCCCAGGCC CCGACGGCCA CGCCACCGCG AGCTTCACAC AGACCCCAGG
    GGAAAAGAGA CTCTGCAAGG TGATGGTGTT GACGGTAAAG AACAAAAGCC AGGAAGTGAG
    GGTGATGAAA AGAGTGATGG AGAAATCAGT CCAGATGGCA GAGGGCTTGA ACAAAGGCAG
    AACAAACCAG ACAACACAGA ATCATCCACA GAGTCCAAAC CAGCAAATTC CCCCCGCAAA
    ATCCCACCTC TTGACTCTGA ACCTGCTACT CTACAGCTCA CTGACAACAC ACTGCAGCAG
    TCTTCACAGG GAACTACATG TGCCGGTGAG ACCGATAACA ATAACCACGG GTCCTTGAAT
    CAGGATAAGT CCTCCCTCAA CACCAGCATC GTGATAGAGG TCACCGACAC TCAGCCATCC
    ATGGAGCTGA CCTCCATTAC GGGTAATAGT AAAGATGTGG AGGCCTCAAA GCCAGAGAAA
    GAAGAAGAAA CCCCAGATGC ACCCAAAGGG CCTCCACCTG TCAGCATGTT CATCTTCAAA
    CCCAACAACC CGGTCCGCAG GGCCTGCCAC TACGTTGTCA ACCTTCGTTA TTTTGAGATG
    TCAATCCTTC TTGTGATCGC CGCCAGCAGT ATCGCATTGG CTGCCGAAGA CCCCGTCAAC
    ACTAATTCGG ACAGGAACAA AGTTCTGCGC TATTTTGACT ATGTATTCAC TGGGGTCTTC
    ACGTTTGAAA TGATAATCAA GATGATTGAT CAGGGGTTGG TCTTACACTA TGGCTCCTAT
    TTTCGTGACC CGTGGAACAT TCTGGATTTC ATAGTTGTGG TGGGAGCTCT GGTGGCTTTT
    GCTCTCACGA ATGTGATGGG GAGTAACACA GGAAGAGACA TTAAAACCAT TAAATCGCTG
    CGTGTACTCC GAGTTCTGAG GCCTCTCAAG ACGATTAAAA GACTTCCTAA ACTCAAGGCT
    GTGTTTGACT GTGTGGTAAC ATCACTGAAG AATGTTTTCA ACATCCTCAT CGTCTACAAG
    CTGTTTATGT TCATATTTGC CGTCATTGCC GTGCAGCTTT TCAAAGGGAA ATTTTACTAC
    TGTACCGACA GTTCCAAGGA CACTGAAAAC GAGTGCAAAG GCTACTACAT TGACTATGAT
    AAAGACAAGA AAAAGGAGAA ACGAGAGTGG AAGAGACGCG ATTTCCATTA TGATAACGTT
    GTCTGGGCTC TGTTGACTCT CTTCACCGTA TCCACTGGCG AAGGCTGGCC GCAAGTTCTG
    CAACACTCTG TGGATGTGAC AGAGGAGGAC CGGGGTCCCA GTCAGGGGAA CAGGATGGAG
    ATGTCTATCT TCTATGTCAT TTATTTTGTG GTGTTCCCCT TCTTCTTCGT CAACATATTT
    GTGGCCCTCA TCATCATCAC TTTCCAAGAA CAGGGTGACA AAATGATGGA GGAGTGTAGT
    CTGGAGAAAA ACGAGAGGGC TTGTATCGAC TTTGCCATCA GTGCCAAACC TTTGACTCGC
    TACATGCCCC AGAACAGACA GACCTTACAG TACAGACTGT GGCAATTTGT GGTGTCGCCC
    TCATTTGAGT ACACAGTACT GACAATGATC GCCCTTAACA CCATCGTTCT CATGATGAAG
    CATCATGATC CTCGTCTTGA AGGCTATGAC GATGTGCTCA AATATCTCAA CACAATTTTC
    ACCGTTATTT TCTCTGTTGA GTGTGTTCTC AAGATCCTGG CTTTTGGTAT AATGAACTAT
    TTCAGAGACA CATGGAATAT ATTTGACTTC ATCACGGTTC TAGGAAGCAT CACAGAAATT
    CTGGTGGACA AATTTCAGAC TGGAAAAACT TTTAATATGG GCTTCCTGAA GCTATTCCGG
    GCAGCTCGGC TGATTAAACT CCTGCGTCAG GGCTACACCA TCCGTATTCT GCTCTGGACT
    TTTGTTCAGT CCTTCAAGGC TCTACCATAT GTCTGTCTGC TCATCGCCAT GCTGTTTTTC
    ATCTATGCCA TCATTGGCAT GCAGGTATTT GGCAACATCA AGATAAATGA GAAAACCCAA
    ATCAATGAAC ACAATAACTT CAGGACTTTC TTTGGTGCAC TCATGCTTTT GTTCAGGAGT
    GCAACAGGTG AATCATGGCA GGAAATCATG CTATCGTGTT TAGGAGAGAA GGAGTGTGAA
    ATTGACACTT CTGTCAACTC AACAACAAAG ACAATAAAAA ACGACTGCGG TACCGACTTT
    GCCTACTTCT ACTTTGTCTC CTTCATCTTC TTCAGTTCTT TTCTGATGCT GAATCTGTTT
    GTGGCTGTGA TCATGGATAA TTTTGAGTAC CTGACACGTG ACTCATCCAT TCTGGGTCCT
    CACCACTTAG ATGAGTTTGT ACGGATCTGG GGAGAGTATG ACCGTGCTGC ATGTGGAAAA
    CTACACTATA AGGAGATGTA CAAAGTAGTA CGCACAATAT CACCTCCTCT GGGCTTTGGA
    AAGAATTGCC CCTACAGGGT GGCATGCAAG CGGTTGGTTC TGATGAACAT GCCAGTGGAT
    GAAGACATGT CTGTCCACTT CACCTCCACA CTTATGGCCC TCATACGCAC CGCACTGGAC
    ATTAAAATTG CTCGAGGAGG GGAAGACAGG GGTCAGATGG ATGTTGAACT GCAGAAGGAA
    ATCAGTGTCA TCTGGCCGCA TCTCTCACAG AAATCTCTTG ATCTGCTTGT ACCCATTCAC
    AAAGCTAGTG ACATGACCAT TGGGAAGATC TATGCAGCCA TGATGATCAT GGACTACTAC
    AAGCAGAACA AAGCCAAGAA GCTCCGTCAG CAGCTCGAGG AACAGAAACA CGCACCAATG
    TTCCAACGCA TGGATGCATC CTCGCTGCCG CAGGACATCC TCTGCAGTGC CAAGGCTTTG
    CCATTCCTGA CTCACAGTGC AGGATCTGCC CTCACTAGAG GTGGGTTTGT GGCACTGAGT
    CCTATCTCTC CTCAAGATCT CTTCATGCAG CCCATGACCC AAGACAGCAT GGAGGAGAGG
    GAGGAATACC AACACCCGTA TCACCTGCAA AGCATGGTGC CTGAGCACAA AGAATTTAGC
    AGTGTGATGT GCCCCACTAG CATGCAGGCT GATCCACTGC AAGTGCCAGG AAGAGGCGTC
    TCTATCCGAG CCTCTTCCAT GCCTCGCCTG GCGGTAGAGC CACAGGCCTC TTTAGAGAAC
    AAACTGATGA AGCGCTCATT CTCCACCATC GGGGATCAGT GTGTAAATGG TGACTGGCAG
    GAAGAGTTCT CTCTGGAGAG GGTGAGTCCT GACCGGCTGG AGAGACCCAG GCAAAGAAGC
    TTCAGAGGTG TCAGGTCAAA CCTGCGGGAG CCAGTGGACA AAGAGAGAGG ACGGTCTAAA
    GAACGAAGAC ACCTGCTGTC TCCAGATGTG TCTCGCTGCA ACTCAGAGGA AAGGAGTCAG
    TCTCAGGAGA GACGTCATTC TCCATCGCCC AGTGAGGGGA GGAAGCACAC AGCTCTCAGA
    CAGGGAAATA ATACAGGCAG CCCTATTGCC TCCACGTCTG ATTCAGGGAC GCCTCGAGGA
    CGCCGCCAAC TCCCACAGAC ACCCTCTCGC CCTCGGCCTC ATATCTCCTA CTCTCCTTTA
    GTGTGCCGTG CCCATCCAGC ACCAGGGGAT GATCGGCCAC GAGCTCCACC CGCAGAGGAA
    GGAGAGGAGC CCACTGCAAC CGGCCCAGGC ACCGCCATGG CCCACTCTAC CCCTCACCGC
    TACATCTCAG AGCCCTACCT GCCACTGCAG GAGGACGTCA ACAGTCCAGA CTCCAGTGGT
    GTGGAGGAGA CTCTCACTTT TGAGGCTGCA ATAGCAACCA GCCTGGGCCG CTCTAATACC
    ATTAGCTCGG CGCCACAGCT ACGTCACTCC TGGCAGGTGC CTAATGGACA TTTCCGCAAA
    CGACTAGCAC AAGCCACAGC TGGGACGTCC GGCTGTGAGC TTCTCAGTGA CACCGATGAG
    GATGACCGCT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212914.2
    CDS388..7722
    Translation

    Target ORF information:

    RefSeq Version XM_017357425.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357425.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTGGAAT TGGGCACTTG TTCGGTCAGC GCTCAGACTC TCTCTGACCG TAATGCCCTG 
    GCGGTCCCGT CATTTGGTCA GGGCATATGT GGCGGGAGCC CACCTTCACG ATGTTTCCTT
    CTCCAGCCGC AGGACCCGGA GAGCTGGCTC AAGCACCGGG GAACCCTGGA GGATGGCTCC
    CGCTCCTGTT CACTCCTGGA GCCTCAGAGC CTGGCTGACA GCCTTCTGTC CCATGCATCC
    AGTTTTGATA TGCTCTACGT CCCACATGGG CGTCCAATGG GCCTTGCACC GTCTTCTCTG
    GATCTGGGCG GCTCGTGTGA GGGGGCGGCC GCGTTGGGTC TGGACCCCGA CATGCCCGTC
    AGCCCGAATA TCATTAAACG GCGCAGGGGG GGACTCATTG AACAACGGGA CATCATTAAA
    GCACACCAGG CACACAAGAT CCAGAGCACC CCACAGGCCC GACGCAAGGA ATGGGAAATG
    GCTCGCTTCG GAGACGATGT TCCCGGTCTG CTGGGAGCCG GGGATGGGGG TTCGGAGCGC
    AGTCGGAACC GGGATGCTCC GGCGGTATCC ACAGGTGGAG TTTCACCAGC CTTCAAACAG
    ACCAAAGCGC AACGAGCCCG AACCATGGCT CTGTACAACC CCATTCCTGT CCGACAGAAC
    TGCTTCACCG TCAACAGATC TCTCTTCATC TTTGGAGAGG ACAACATCGT TAGGAAGTAT
    GCCAAGAAGA TCATAGAGTG GCCTCCGTTT GAATACATGA TCCTGGCCAC CATTATTGCT
    AACTGCATCG TCCTAGCCTT GGAGCAGCAC CTGCCTGGAG AAGACAAGAC ACCCATGGCT
    AAGAGACTTG AAAAAACAGA GCCCTACTTC ATAGGAATGT TTTGCTTTGA AGCTGGAATA
    AAAATCATTG CTTTGGGATT TGTATTCCAC AAGGGCTCGT ACCTGAGGAA TGGTTGGAAC
    GTCATGGACT TTATTGTCGT ATCGAGTGGG ATTCTGGCCA CAGCAGGAGC CCACATGAAT
    ATTTCATTAG ACCTGCGCAC ACTGAGGGCT GTGAGGGTAT TACGGCCCCT CAAACTGGTC
    TCTGGCATTC CTAGTCTGCA AATAGTTCTT AAATCCATAA TGAAGGCAAT GGTGCCATTG
    CTTCAGATCG GACTGCTACT GTTTTTTGCT ATTCTAATGT TTGCCATTAT TGGATTAGAG
    TTCTACAGTG GGAAACTCCA CAAGCAATGT GAACCTGTAG GTAAAGATTC TGAGAGAGCA
    GACTCGCCTG AGAATGAATT TCCATGTGGA AGCAAACTGT GTCCTGACCA TTATAACTGC
    TCTGGCTACT GGACGGGACC CAATGATGGC ATCACTCAGT TTGATAACAT CTTATTTTCT
    TTACTCACCG TCTTCCAGTG CATCACCATG GAAGGCTGGA CGACCATCCT CTATTACACC
    AACGATGCCT TAGGCTCTAC TTGGAATTGG CTCTACTTCA TTCCTCTAAT CATAATTGGC
    TCCTTCTTTG TGTTGAATCT GGTATTAGGA GTCCTGTCAG GGGAGTTTGC TAAAGAGCGA
    GAGCGGGTAG AGAACCGGAG AGCCTTCATG AAGCTTAGGA GACAGCAGCA GATTGAGAGG
    GAGCTGAACG GATATCGGGC CTGGATCGAC CGTGCAGAGG AGGTTATGCT TGCAGAGGAG
    AACAAGAATT CAGGACCTTC TGCTTTGGAT GTGCTAAAAA GAGCTACCAC CATAAAGAGA
    AGAGGAGTGG ATGATGCTCG AAGAGCTCCA GAAGAGCAGT ATGCTGACAT CTCATCTCTA
    GGGATGCCAC TGAACAGAGC CAGCATACGC AGTGCTAAAA GAGGACCAAC ATCCTATTTC
    CGACGGAAAG AGCGCCTGCT ACGGATCTCC GTCCGCCGTA TGGTGAAGAC TCAGACGTTC
    TACTGGACTG TTCTGGGTCT GGTAGCCCTC AACACTCTCT GTGTAGCCAT CGTACATCAC
    AAACAGCCAC AGTGGTTATC TAGTCTCCTC TACTATGCAG AGTTTTTGTT TCTGGGTTTG
    TTTCTTACCG AGATGTTTCT GAAGATGTAT GGACTCGGAC CCAGACTCTA TTTTCACTCC
    TCCTTCAACT GCTTCGATTG TGGGGTTATA GTAGGCAGCA TCTTTGAGGT GTTCTGGGGC
    TTCTTCAGAC CTGGCACATC CTTTGGGATC AGCGTTCTTC GTGCCTTGCG GTTGTTGAGA
    ATTTTCAAGA TAACCAAATA CTGGGCGTCC CTGAGGAACC TGGTCGTGTC TCTGATGAAC
    TCAATGAAGT CTATTATCAG TTTGCTCTTC CTGCTTTTTC TCTTTATTGT GGTCTTTGCA
    CTTCTGGGCA TGCAGCTCTT TGGTGGAAGG TTCATTTTTG ATGAGTACAC TCCCACCAAT
    TTTGACACTT TCCCAGCAGC CATCATGACA GTGTTTCAAA TTCTGACTGG TGAGGACTGG
    AATGAAGTCA TGTACAATGG CATCCGGTCT CAAGGTGGCG TGAAATCTGG GATGTGGTCG
    TCTGTCTACT TCATTGTTCT CACTCTGTTT GGAAATTATA CGCTGCTTAA CGTCTTCTTG
    GCTATCGCTG TCGACAATTT GGCCAATGCC CAGGAGCTCA CAAAGGACGA AGAGGAGGAG
    GAAGAGGCGT TTAATCAGAA ACATGCCCTG CAGAAAGCCA AGGAGGTTGG GCCCATGTCC
    TCTTTCATGT CCTCAGAGAG GAGGAGGAGG CCCTACCTGT ACAGGAAGCG TGCAATTCAT
    CGGAGCCGTG CAGCCTACTC CTTCGAGGAT GCAGAGAGTC TGGCTGACGC TCGTCCATTC
    AATGCCTCTA ACTCTTTCAT GTCCAGGAGA GAGAGGAGGA GGAGGTTGAC CATGTCAGTG
    TGGGAGCAGA GGACCAGCCA GCTGCGGCGG CACAGGCAGA TGTCCAGCCG CGAGATCCTT
    TTCAGCAGCC CCAGTGAAGA ACAGGACGGA CCACCAGGCT GCCCTCTCCA CCGGAAACCT
    GGACTATCTC CATCTGGCAG CAAGCGCAGA GCCACTGAGA CCACTTCAAC CACAATCATG
    CCAGAGGGTA CCTTAGAGCC ACCCATGCCT ATGGACATGC CGGTGGATGA GCAAGCTCTG
    CCCATTTCTA TTCCTGAACC CCCTTTAGCC ATTCCAATTC CAGAGCCACC AATGCCCGTG
    GACGCATGCC TCCCTGAACC CCCAGTTACA CTGAATCTGC CCTTAACAGA GCCATCTGAA
    TCAAAGACTT CACCCGAGGG TGCTCTGGAC ATCAACAACC ATTGCCCTAG ACTAAATGGG
    AACAGGCGGC AGCGGGCTGT CCGTAAGCAC AGACCTCCTG CTATGGGGGA CATGCTGACC
    CCTGATATGG GGCCCAGGCC CCGACGGCCA CGCCACCGCG AGCTTCACAC AGACCCCAGG
    GGAAAAGAGA CTCTGCAAGG TGATGGTGTT GACGGTAAAG AACAAAAGCC AGGAAGTGAG
    GGTGATGAAA AGAGTGATGG AGAAATCAGT CCAGATGGCA GAGGGCTTGA ACAAAGGCAG
    AACAAACCAG ACAACACAGA ATCATCCACA GAGTCCAAAC CAGCAAATTC CCCCCGCAAA
    ATCCCACCTC TTGACTCTGA ACCTGCTACT CTACAGCTCA CTGACAACAC ACTGCAGCAG
    TCTTCACAGG GAACTACATG TGCCGGTGAG ACCGATAACA ATAACCACGG GTCCTTGAAT
    CAGGATAAGT CCTCCCTCAA CACCAGCATC GTGATAGAGG TCACCGACAC TCAGCCATCC
    ATGGAGCTGA CCTCCATTAC GGGTAATAGT AAAGATGTGG AGGCCTCAAA GCCAGAGAAA
    GAAGAAGAAA CCCCAGATGC ACCCAAAGGG CCTCCACCTG TCAGCATGTT CATCTTCAAA
    CCCAACAACC CGGTCCGCAG GGCCTGCCAC TACGTTGTCA ACCTTCGTTA TTTTGAGATG
    TCAATCCTTC TTGTGATCGC CGCCAGCAGT ATCGCATTGG CTGCCGAAGA CCCCGTCAAC
    ACTAATTCGG ACAGGAACAA AGTTCTGCGC TATTTTGACT ATGTATTCAC TGGGGTCTTC
    ACGTTTGAAA TGATAATCAA GATGATTGAT CAGGGGTTGG TCTTACACTA TGGCTCCTAT
    TTTCGTGACC CGTGGAACAT TCTGGATTTC ATAGTTGTGG TGGGAGCTCT GGTGGCTTTT
    GCTCTCACGA ATGTGATGGG GAGTAACACA GGAAGAGACA TTAAAACCAT TAAATCGCTG
    CGTGTACTCC GAGTTCTGAG GCCTCTCAAG ACGATTAAAA GACTTCCTAA ACTCAAGGCT
    GTGTTTGACT GTGTGGTAAC ATCACTGAAG AATGTTTTCA ACATCCTCAT CGTCTACAAG
    CTGTTTATGT TCATATTTGC CGTCATTGCC GTGCAGCTTT TCAAAGGGAA ATTTTACTAC
    TGTACCGACA GTTCCAAGGA CACTGAAAAC GAGTGCAAAG GCTACTACAT TGACTATGAT
    AAAGACAAGA AAAAGGAGAA ACGAGAGTGG AAGAGACGCG ATTTCCATTA TGATAACGTT
    GTCTGGGCTC TGTTGACTCT CTTCACCGTA TCCACTGGCG AAGGCTGGCC GCAAGTTCTG
    CAACACTCTG TGGATGTGAC AGAGGAGGAC CGGGGTCCCA GTCAGGGGAA CAGGATGGAG
    ATGTCTATCT TCTATGTCAT TTATTTTGTG GTGTTCCCCT TCTTCTTCGT CAACATATTT
    GTGGCCCTCA TCATCATCAC TTTCCAAGAA CAGGGTGACA AAATGATGGA GGAGTGTAGT
    CTGGAGAAAA ACGAGAGGGC TTGTATCGAC TTTGCCATCA GTGCCAAACC TTTGACTCGC
    TACATGCCCC AGAACAGACA GACCTTACAG TACAGACTGT GGCAATTTGT GGTGTCGCCC
    TCATTTGAGT ACACAGTACT GACAATGATC GCCCTTAACA CCATCGTTCT CATGATGAAG
    CATCATGATC CTCGTCTTGA AGGCTATGAC GATGTGCTCA AATATCTCAA CACAATTTTC
    ACCGTTATTT TCTCTGTTGA GTGTGTTCTC AAGATCCTGG CTTTTGGTAT AATGAACTAT
    TTCAGAGACA CATGGAATAT ATTTGACTTC ATCACGGTTC TAGGAAGCAT CACAGAAATT
    CTGGTGGACA AATTTCAGAC TGGAAAAACT TTTAATATGG GCTTCCTGAA GCTATTCCGG
    GCAGCTCGGC TGATTAAACT CCTGCGTCAG GGCTACACCA TCCGTATTCT GCTCTGGACT
    TTTGTTCAGT CCTTCAAGGC TCTACCATAT GTCTGTCTGC TCATCGCCAT GCTGTTTTTC
    ATCTATGCCA TCATTGGCAT GCAGGTATTT GGCAACATCA AGATAAATGA GAAAACCCAA
    ATCAATGAAC ACAATAACTT CAGGACTTTC TTTGGTGCAC TCATGCTTTT GTTCAGGAGT
    GCAACAGGTG AATCATGGCA GGAAATCATG CTATCGTGTT TAGGAGAGAA GGAGTGTGAA
    ATTGACACTT CTGTCAACTC AACAACAAAG ACAATAAAAA ACGACTGCGG TACCGACTTT
    GCCTACTTCT ACTTTGTCTC CTTCATCTTC TTCAGTTCTT TTCTGATGCT GAATCTGTTT
    GTGGCTGTGA TCATGGATAA TTTTGAGTAC CTGACACGTG ACTCATCCAT TCTGGGTCCT
    CACCACTTAG ATGAGTTTGT ACGGATCTGG GGAGAGTATG ACCGTGCTGC ATGTGGAAAA
    CTACACTATA AGGAGATGTA CAAAGTAGTA CGCACAATAT CACCTCCTCT GGGCTTTGGA
    AAGAATTGCC CCTACAGGGT GGCATGCAAG CGGTTGGTTC TGATGAACAT GCCAGTGGAT
    GAAGACATGT CTGTCCACTT CACCTCCACA CTTATGGCCC TCATACGCAC CGCACTGGAC
    ATTAAAATTG CTCGAGGAGG GGAAGACAGG GGTCAGATGG ATGTTGAACT GCAGAAGGAA
    ATCAGTGTCA TCTGGCCGCA TCTCTCACAG AAATCTCTTG ATCTGCTTGT ACCCATTCAC
    AAAGCTAGTG ACATGACCAT TGGGAAGATC TATGCAGCCA TGATGATCAT GGACTACTAC
    AAGCAGAACA AAGCCAAGAA GCTCCGTCAG CAGCTCGAGG AACAGAAACA CGCACCAATG
    TTCCAACGCA TGGATGCATC CTCGCTGCCG CAGGACATCC TCTGCAGTGC CAAGGCTTTG
    CCATTCCTGA CTCACAGTGC AGGATCTGCC CTCACTAGAG GTGGGTTTGT GGCACTGAGT
    CCTATCTCTC CTCAAGATCT CTTCATGCAG CCCATGACCC AAGACAGCAT GGAGGAGAGG
    GAGGAATACC AACACCCGTA TCACCTGCAA AGCATGGTGC CTGAGCACAA AGAATTTAGC
    AGTGTGATGT GCCCCACTAG CATGCAGGCT GATCCACTGC AAGTGCCAGG AAGAGGCGTC
    TCTATCCGAG CCTCTTCCAT GCCTCGCCTG GCGGTAGAGC CACAGGCCTC TTTAGAGAAC
    AAACTGATGA AGCGCTCATT CTCCACCATC GGGGATCAGT GTGTAAATGG TGACTGGCAG
    GAAGAGTTCT CTCTGGAGAG GGTGAGTCCT GACCGGCTGG AGAGACCCAG GCAAAGAAGC
    TTCAGAGGTG TCAGGTCAAA CCTGCGGGAG CCAGTGGACA AAGAGAGAGG ACGGTCTAAA
    GAACGAAGAC ACCTGCTGTC TCCAGATGTG TCTCGCTGCA ACTCAGAGGA AAGGAGTCAG
    TCTCAGGAGA GACGTCATTC TCCATCGCCC AGTGAGGGGA GGAAGCACAC AGCTCTCAGA
    CAGGGAAATA ATACAGGCAG CCCTATTGCC TCCACGTCTG ATTCAGGGAC GCCTCGAGGA
    CGCCGCCAAC TCCCACAGAC ACCCTCTCGC CCTCGGCCTC ATATCTCCTA CTCTCCTTTA
    GTGTGCCGTG CCCATCCAGC ACCAGGGGAT GATCGGCCAC GAGCTCCACC CGCAGAGGAA
    GGAGAGGAGC CCACTGCAAC CGGCCCAGGC ACCGCCATGG CCCACTCTAC CCCTCACCGC
    TACATCTCAG AGCCCTACCT GCCACTGCAG GAGGACGTCA ACAGTCCAGA CTCCAGTGGT
    GTGGAGGAGA CTCTCACTTT TGAGGCTGCA ATAGCAACCA GCCTGGGCCG CTCTAATACC
    ATTAGCTCGG CGCCACAGCT ACGTCACTCC TGGCAGGTGC CTAATGGACA TTTCCGCAAA
    CGACTAGCAC AAGCCACAGC TGGGACGTCC GGCTGTGAGC TTCTCAGTGA CACCGATGAG
    GATGACCGCT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44278
    Clone ID Related Accession (Same CDS sequence) XM_021478386.1
    Accession Version XM_021478386.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
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    3421
    3481
    3541
    3601
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    3901
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    4141
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    4381
    4441
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    4681
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    4981
    5041
    5101
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    5401
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    5701
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    5881
    5941
    6001
    6061
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    6181
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    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTGGAAT TGGGCACTTG TTCGGTCAGC GCTCAGACTC TCTCTGACCG TAATGCCCTG 
    GCGGTCCCGT CATTTGGTCA GGGCATATGT GGCGGGAGCC CACCTTCACG ATGTTTCCTT
    CTCCAGCCGC AGGACCCGGA GAGCTGGCTC AAGCACCGGG GAACCCTGGA GGATGGCTCC
    CGCTCCTGTT CACTCCTGGA GCCTCAGAGC CTGGCTGACA GCCTTCTGTC CCATGCATCC
    AGTTTTGATA TGCTCTACGT CCCACATGGG CGTCCAATGG GCCTTGCACC GTCTTCTCTG
    GATCTGGGCG GCTCGTGTGA GGGGGCGGCC GCGTTGGGTC TGGACCCCGA CATGCCCGTC
    AGCCCGAATA TCATTAAACG GCGCAGGGGG GGACTCATTG AACAACGGGA CATCATTAAA
    GCACACCAGG CACACAAGAT CCAGAGCACC CCACAGGCCC GACGCAAGGA ATGGGAAATG
    GCTCGCTTCG GAGACGATGT TCCCGGTCTG CTGGGAGCCG GGGATGGGGG TTCGGAGCGC
    AGTCGGAACC GGGATGCTCC GGCGGTATCC ACAGGTGGAG TTTCACCAGC CTTCAAACAG
    ACCAAAGCGC AACGAGCCCG AACCATGGCT CTGTACAACC CCATTCCTGT CCGACAGAAC
    TGCTTCACCG TCAACAGATC TCTCTTCATC TTTGGAGAGG ACAACATCGT TAGGAAGTAT
    GCCAAGAAGA TCATAGAGTG GCCTCCGTTT GAATACATGA TCCTGGCCAC CATTATTGCT
    AACTGCATCG TCCTAGCCTT GGAGCAGCAC CTGCCTGGAG AAGACAAGAC ACCCATGGCT
    AAGAGACTTG AAAAAACAGA GCCCTACTTC ATAGGAATGT TTTGCTTTGA AGCTGGAATA
    AAAATCATTG CTTTGGGATT TGTATTCCAC AAGGGCTCGT ACCTGAGGAA TGGTTGGAAC
    GTCATGGACT TTATTGTCGT ATCGAGTGGG ATTCTGGCCA CAGCAGGAGC CCACATGAAT
    ATTTCATTAG ACCTGCGCAC ACTGAGGGCT GTGAGGGTAT TACGGCCCCT CAAACTGGTC
    TCTGGCATTC CTAGTCTGCA AATAGTTCTT AAATCCATAA TGAAGGCAAT GGTGCCATTG
    CTTCAGATCG GACTGCTACT GTTTTTTGCT ATTCTAATGT TTGCCATTAT TGGATTAGAG
    TTCTACAGTG GGAAACTCCA CAAGCAATGT GAACCTGTAG GTAAAGATTC TGAGAGAGCA
    GACTCGCCTG AGAATGAATT TCCATGTGGA AGCAAACTGT GTCCTGACCA TTATAACTGC
    TCTGGCTACT GGACGGGACC CAATGATGGC ATCACTCAGT TTGATAACAT CTTATTTTCT
    TTACTCACCG TCTTCCAGTG CATCACCATG GAAGGCTGGA CGACCATCCT CTATTACACC
    AACGATGCCT TAGGCTCTAC TTGGAATTGG CTCTACTTCA TTCCTCTAAT CATAATTGGC
    TCCTTCTTTG TGTTGAATCT GGTATTAGGA GTCCTGTCAG GGGAGTTTGC TAAAGAGCGA
    GAGCGGGTAG AGAACCGGAG AGCCTTCATG AAGCTTAGGA GACAGCAGCA GATTGAGAGG
    GAGCTGAACG GATATCGGGC CTGGATCGAC CGTGCAGAGG AGGTTATGCT TGCAGAGGAG
    AACAAGAATT CAGGACCTTC TGCTTTGGAT GTGCTAAAAA GAGCTACCAC CATAAAGAGA
    AGAGGAGTGG ATGATGCTCG AAGAGCTCCA GAAGAGCAGT ATGCTGACAT CTCATCTCTA
    GGGATGCCAC TGAACAGAGC CAGCATACGC AGTGCTAAAA GAGGACCAAC ATCCTATTTC
    CGACGGAAAG AGCGCCTGCT ACGGATCTCC GTCCGCCGTA TGGTGAAGAC TCAGACGTTC
    TACTGGACTG TTCTGGGTCT GGTAGCCCTC AACACTCTCT GTGTAGCCAT CGTACATCAC
    AAACAGCCAC AGTGGTTATC TAGTCTCCTC TACTATGCAG AGTTTTTGTT TCTGGGTTTG
    TTTCTTACCG AGATGTTTCT GAAGATGTAT GGACTCGGAC CCAGACTCTA TTTTCACTCC
    TCCTTCAACT GCTTCGATTG TGGGGTTATA GTAGGCAGCA TCTTTGAGGT GTTCTGGGGC
    TTCTTCAGAC CTGGCACATC CTTTGGGATC AGCGTTCTTC GTGCCTTGCG GTTGTTGAGA
    ATTTTCAAGA TAACCAAATA CTGGGCGTCC CTGAGGAACC TGGTCGTGTC TCTGATGAAC
    TCAATGAAGT CTATTATCAG TTTGCTCTTC CTGCTTTTTC TCTTTATTGT GGTCTTTGCA
    CTTCTGGGCA TGCAGCTCTT TGGTGGAAGG TTCATTTTTG ATGAGTACAC TCCCACCAAT
    TTTGACACTT TCCCAGCAGC CATCATGACA GTGTTTCAAA TTCTGACTGG TGAGGACTGG
    AATGAAGTCA TGTACAATGG CATCCGGTCT CAAGGTGGCG TGAAATCTGG GATGTGGTCG
    TCTGTCTACT TCATTGTTCT CACTCTGTTT GGAAATTATA CGCTGCTTAA CGTCTTCTTG
    GCTATCGCTG TCGACAATTT GGCCAATGCC CAGGAGCTCA CAAAGGACGA AGAGGAGGAG
    GAAGAGGCGT TTAATCAGAA ACATGCCCTG CAGAAAGCCA AGGAGGTTGG GCCCATGTCC
    TCTTTCATGT CCTCAGAGAG GAGGAGGAGG CCCTACCTGT ACAGGAAGCG TGCAATTCAT
    CGGAGCCGTG CAGCCTACTC CTTCGAGGAT GCAGAGAGTC TGGCTGACGC TCGTCCATTC
    AATGCCTCTA ACTCTTTCAT GTCCAGGAGA GAGAGGAGGA GGAGGTTGAC CATGTCAGTG
    TGGGAGCAGA GGACCAGCCA GCTGCGGCGG CACAGGCAGA TGTCCAGCCG CGAGATCCTT
    TTCAGCAGCC CCAGTGAAGA ACAGGACGGA CCACCAGGCT GCCCTCTCCA CCGGAAACCT
    GGACTATCTC CATCTGGCAG CAAGCGCAGA GCCACTGAGA CCACTTCAAC CACAATCATG
    CCAGAGGGTA CCTTAGAGCC ACCCATGCCT ATGGACATGC CGGTGGATGA GCAAGCTCTG
    CCCATTTCTA TTCCTGAACC CCCTTTAGCC ATTCCAATTC CAGAGCCACC AATGCCCGTG
    GACGCATGCC TCCCTGAACC CCCAGTTACA CTGAATCTGC CCTTAACAGA GCCATCTGAA
    TCAAAGACTT CACCCGAGGG TGCTCTGGAC ATCAACAACC ATTGCCCTAG ACTAAATGGG
    AACAGGCGGC AGCGGGCTGT CCGTAAGCAC AGACCTCCTG CTATGGGGGA CATGCTGACC
    CCTGATATGG GGCCCAGGCC CCGACGGCCA CGCCACCGCG AGCTTCACAC AGACCCCAGG
    GGAAAAGAGA CTCTGCAAGG TGATGGTGTT GACGGTAAAG AACAAAAGCC AGGAAGTGAG
    GGTGATGAAA AGAGTGATGG AGAAATCAGT CCAGATGGCA GAGGGCTTGA ACAAAGGCAG
    AACAAACCAG ACAACACAGA ATCATCCACA GAGTCCAAAC CAGCAAATTC CCCCCGCAAA
    ATCCCACCTC TTGACTCTGA ACCTGCTACT CTACAGCTCA CTGACAACAC ACTGCAGCAG
    TCTTCACAGG GAACTACATG TGCCGGTGAG ACCGATAACA ATAACCACGG GTCCTTGAAT
    CAGGATAAGT CCTCCCTCAA CACCAGCATC GTGATAGAGG TCACCGACAC TCAGCCATCC
    ATGGAGCTGA CCTCCATTAC GGGTAATAGT AAAGATGTGG AGGCCTCAAA GCCAGAGAAA
    GAAGAAGAAA CCCCAGATGC ACCCAAAGGG CCTCCACCTG TCAGCATGTT CATCTTCAAA
    CCCAACAACC CGGTCCGCAG GGCCTGCCAC TACGTTGTCA ACCTTCGTTA TTTTGAGATG
    TCAATCCTTC TTGTGATCGC CGCCAGCAGT ATCGCATTGG CTGCCGAAGA CCCCGTCAAC
    ACTAATTCGG ACAGGAACAA AGTTCTGCGC TATTTTGACT ATGTATTCAC TGGGGTCTTC
    ACGTTTGAAA TGATAATCAA GATGATTGAT CAGGGGTTGG TCTTACACTA TGGCTCCTAT
    TTTCGTGACC CGTGGAACAT TCTGGATTTC ATAGTTGTGG TGGGAGCTCT GGTGGCTTTT
    GCTCTCACGA GTAACACAGG AAGAGACATT AAAACCATTA AATCGCTGCG TGTACTCCGA
    GTTCTGAGGC CTCTCAAGAC GATTAAAAGA CTTCCTAAAC TCAAGGCTGT GTTTGACTGT
    GTGGTAACAT CACTGAAGAA TGTTTTCAAC ATCCTCATCG TCTACAAGCT GTTTATGTTC
    ATATTTGCCG TCATTGCCGT GCAGCTTTTC AAAGGGAAAT TTTACTACTG TACCGACAGT
    TCCAAGGACA CTGAAAACGA GTGCAAAGGC TACTACATTG ACTATGATAA AGACAAGAAA
    AAGGAGAAAC GAGAGTGGAA GAGACGCGAT TTCCATTATG ATAACGTTGT CTGGGCTCTG
    TTGACTCTCT TCACCGTATC CACTGGCGAA GGCTGGCCGC AAGTTCTGCA ACACTCTGTG
    GATGTGACAG AGGAGGACCG GGGTCCCAGT CAGGGGAACA GGATGGAGAT GTCTATCTTC
    TATGTCATTT ATTTTGTGGT GTTCCCCTTC TTCTTCGTCA ACATATTTGT GGCCCTCATC
    ATCATCACTT TCCAAGAACA GGGTGACAAA ATGATGGAGG AGTGTAGTCT GGAGAAAAAC
    GAGAGGGCTT GTATCGACTT TGCCATCAGT GCCAAACCTT TGACTCGCTA CATGCCCCAG
    AACAGACAGA CCTTACAGTA CAGACTGTGG CAATTTGTGG TGTCGCCCTC ATTTGAGTAC
    ACAGTACTGA CAATGATCGC CCTTAACACC ATCGTTCTCA TGATGAAGCA TCATGATCCT
    CGTCTTGAAG GCTATGACGA TGTGCTCAAA TATCTCAACA CAATTTTCAC CGTTATTTTC
    TCTGTTGAGT GTGTTCTCAA GATCCTGGCT TTTGGTATAA TGAACTATTT CAGAGACACA
    TGGAATATAT TTGACTTCAT CACGGTTCTA GGAAGCATCA CAGAAATTCT GGTGGACAAA
    TTTCAGACTG GAAAAACTTT TAATATGGGC TTCCTGAAGC TATTCCGGGC AGCTCGGCTG
    ATTAAACTCC TGCGTCAGGG CTACACCATC CGTATTCTGC TCTGGACTTT TGTTCAGTCC
    TTCAAGGCTC TACCATATGT CTGTCTGCTC ATCGCCATGC TGTTTTTCAT CTATGCCATC
    ATTGGCATGC AGGTATTTGG CAACATCAAG ATAAATGAGA AAACCCAAAT CAATGAACAC
    AATAACTTCA GGACTTTCTT TGGTGCACTC ATGCTTTTGT TCAGGAGTGC AACAGGTGAA
    TCATGGCAGG AAATCATGCT ATCGTGTTTA GGAGAGAAGG AGTGTGAAAT TGACACTTCT
    GTCAACTCAA CAACAAAGAC AATAAAAAAC GACTGCGGTA CCGACTTTGC CTACTTCTAC
    TTTGTCTCCT TCATCTTCTT CAGTTCTTTT CTGATGCTGA ATCTGTTTGT GGCTGTGATC
    ATGGATAATT TTGAGTACCT GACACGTGAC TCATCCATTC TGGGTCCTCA CCACTTAGAT
    GAGTTTGTAC GGATCTGGGG AGAGTATGAC CGTGCTGCAT GTGGCCGTAT TCATTACACT
    GATATGTACG AGATGCTGAC CTGCATGTCG CCACCGCTTG GCCTGGGCAA GAAATGTCCC
    TCCAAAGTGG CGTATAAGCG GTTGGTTCTG ATGAACATGC CAGTGGATGA AGACATGTCT
    GTCCACTTCA CCTCCACACT TATGGCCCTC ATACGCACCG CACTGGACAT TAAAATTGCT
    CGAGGAGGGG AAGACAGGGG TCAGATGGAT GTTGAACTGC AGAAGGAAAT CAGTGTCATC
    TGGCCGCATC TCTCACAGAA ATCTCTTGAT CTGCTTGTAC CCATTCACAA AGCTAGTGAC
    ATGACCATTG GGAAGATCTA TGCAGCCATG ATGATCATGG ACTACTACAA GCAGAACAAA
    GCCAAGAAGC TCCGTCAGCA GCTCGAGGAA CAGAAACACG CACCAATGTT CCAACGCATG
    GATGCATCCT CGCTGCCGCA GGACATCCTC TGCAGTGCCA AGGCTTTGCC ATTCCTGACT
    CACAGTGCAG GATCTGCCCT CACTAGAGGT GGGTTTGTGG CACTGAGTCC TATCTCTCCT
    CAAGATCTCT TCATGCAGCC CATGACCCAA GACAGCATGG AGGAGAGGGA GGAATACCAA
    CACCCGTATC ACCTGCAAAG CATGGTGCCT GAGCACAAAG AATTTAGCAG TGTGATGTGC
    CCCACTAGCA TGCAGGCTGA TCCACTGCAA GTGCCAGGAA GAGGCGTCTC TATCCGAGCC
    TCTTCCATGC CTCGCCTGGC GGTAGAGCCA CAGGCCTCTT TAGAGAACAA ACTGATGAAG
    CGCTCATTCT CCACCATCGG GGATCAGTGT GTAAATGGTG ACTGGCAGGA AGAGTTCTCT
    CTGGAGAGGG TGAGTCCTGA CCGGCTGGAG AGACCCAGGC AAAGAAGCTT CAGAGGTGTC
    AGGTCAAACC TGCGGGAGCC AGTGGACAAA GAGAGAGGAC GGTCTAAAGA ACGAAGACAC
    CTGCTGTCTC CAGATGTGTC TCGCTGCAAC TCAGAGGAAA GGAGTCAGTC TCAGGAGAGA
    CGTCATTCTC CATCGCCCAG TGAGGGGAGG AAGCACACAG CTCTCAGACA GGGAAATAAT
    ACAGGCAGCC CTATTGCCTC CACGTCTGAT TCAGGGACGC CTCGAGGACG CCGCCAACTC
    CCACAGACAC CCTCTCGCCC TCGGCCTCAT ATCTCCTACT CTCCTTTAGT GTGCCGTGCC
    CATCCAGCAC CAGGGGATGA TCGGCCACGA GCTCCACCCG CAGAGGAAGG AGAGGAGCCC
    ACTGCAACCG GCCCAGGCAC CGCCATGGCC CACTCTACCC CTCACCGCTA CATCTCAGAG
    CCCTACCTGC CACTGCAGGA GGACGTCAAC AGTCCAGACT CCAGTGGTGT GGAGGAGACT
    CTCACTTTTG AGGCTGCAAT AGCAACCAGC CTGGGCCGCT CTAATACCAT TAGCTCGGCG
    CCACAGCTAC GTCACTCCTG GCAGGTGCCT AATGGACATT TCCGCAAACG ACTAGCACAA
    GCCACAGCTG GGACGTCCGG CTGTGAGCTT CTCAGTGACA CCGATGAGGA TGACCGCTGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334061.1
    CDS388..7710
    Translation

    Target ORF information:

    RefSeq Version XM_021478386.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478386.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTGGAAT TGGGCACTTG TTCGGTCAGC GCTCAGACTC TCTCTGACCG TAATGCCCTG 
    GCGGTCCCGT CATTTGGTCA GGGCATATGT GGCGGGAGCC CACCTTCACG ATGTTTCCTT
    CTCCAGCCGC AGGACCCGGA GAGCTGGCTC AAGCACCGGG GAACCCTGGA GGATGGCTCC
    CGCTCCTGTT CACTCCTGGA GCCTCAGAGC CTGGCTGACA GCCTTCTGTC CCATGCATCC
    AGTTTTGATA TGCTCTACGT CCCACATGGG CGTCCAATGG GCCTTGCACC GTCTTCTCTG
    GATCTGGGCG GCTCGTGTGA GGGGGCGGCC GCGTTGGGTC TGGACCCCGA CATGCCCGTC
    AGCCCGAATA TCATTAAACG GCGCAGGGGG GGACTCATTG AACAACGGGA CATCATTAAA
    GCACACCAGG CACACAAGAT CCAGAGCACC CCACAGGCCC GACGCAAGGA ATGGGAAATG
    GCTCGCTTCG GAGACGATGT TCCCGGTCTG CTGGGAGCCG GGGATGGGGG TTCGGAGCGC
    AGTCGGAACC GGGATGCTCC GGCGGTATCC ACAGGTGGAG TTTCACCAGC CTTCAAACAG
    ACCAAAGCGC AACGAGCCCG AACCATGGCT CTGTACAACC CCATTCCTGT CCGACAGAAC
    TGCTTCACCG TCAACAGATC TCTCTTCATC TTTGGAGAGG ACAACATCGT TAGGAAGTAT
    GCCAAGAAGA TCATAGAGTG GCCTCCGTTT GAATACATGA TCCTGGCCAC CATTATTGCT
    AACTGCATCG TCCTAGCCTT GGAGCAGCAC CTGCCTGGAG AAGACAAGAC ACCCATGGCT
    AAGAGACTTG AAAAAACAGA GCCCTACTTC ATAGGAATGT TTTGCTTTGA AGCTGGAATA
    AAAATCATTG CTTTGGGATT TGTATTCCAC AAGGGCTCGT ACCTGAGGAA TGGTTGGAAC
    GTCATGGACT TTATTGTCGT ATCGAGTGGG ATTCTGGCCA CAGCAGGAGC CCACATGAAT
    ATTTCATTAG ACCTGCGCAC ACTGAGGGCT GTGAGGGTAT TACGGCCCCT CAAACTGGTC
    TCTGGCATTC CTAGTCTGCA AATAGTTCTT AAATCCATAA TGAAGGCAAT GGTGCCATTG
    CTTCAGATCG GACTGCTACT GTTTTTTGCT ATTCTAATGT TTGCCATTAT TGGATTAGAG
    TTCTACAGTG GGAAACTCCA CAAGCAATGT GAACCTGTAG GTAAAGATTC TGAGAGAGCA
    GACTCGCCTG AGAATGAATT TCCATGTGGA AGCAAACTGT GTCCTGACCA TTATAACTGC
    TCTGGCTACT GGACGGGACC CAATGATGGC ATCACTCAGT TTGATAACAT CTTATTTTCT
    TTACTCACCG TCTTCCAGTG CATCACCATG GAAGGCTGGA CGACCATCCT CTATTACACC
    AACGATGCCT TAGGCTCTAC TTGGAATTGG CTCTACTTCA TTCCTCTAAT CATAATTGGC
    TCCTTCTTTG TGTTGAATCT GGTATTAGGA GTCCTGTCAG GGGAGTTTGC TAAAGAGCGA
    GAGCGGGTAG AGAACCGGAG AGCCTTCATG AAGCTTAGGA GACAGCAGCA GATTGAGAGG
    GAGCTGAACG GATATCGGGC CTGGATCGAC CGTGCAGAGG AGGTTATGCT TGCAGAGGAG
    AACAAGAATT CAGGACCTTC TGCTTTGGAT GTGCTAAAAA GAGCTACCAC CATAAAGAGA
    AGAGGAGTGG ATGATGCTCG AAGAGCTCCA GAAGAGCAGT ATGCTGACAT CTCATCTCTA
    GGGATGCCAC TGAACAGAGC CAGCATACGC AGTGCTAAAA GAGGACCAAC ATCCTATTTC
    CGACGGAAAG AGCGCCTGCT ACGGATCTCC GTCCGCCGTA TGGTGAAGAC TCAGACGTTC
    TACTGGACTG TTCTGGGTCT GGTAGCCCTC AACACTCTCT GTGTAGCCAT CGTACATCAC
    AAACAGCCAC AGTGGTTATC TAGTCTCCTC TACTATGCAG AGTTTTTGTT TCTGGGTTTG
    TTTCTTACCG AGATGTTTCT GAAGATGTAT GGACTCGGAC CCAGACTCTA TTTTCACTCC
    TCCTTCAACT GCTTCGATTG TGGGGTTATA GTAGGCAGCA TCTTTGAGGT GTTCTGGGGC
    TTCTTCAGAC CTGGCACATC CTTTGGGATC AGCGTTCTTC GTGCCTTGCG GTTGTTGAGA
    ATTTTCAAGA TAACCAAATA CTGGGCGTCC CTGAGGAACC TGGTCGTGTC TCTGATGAAC
    TCAATGAAGT CTATTATCAG TTTGCTCTTC CTGCTTTTTC TCTTTATTGT GGTCTTTGCA
    CTTCTGGGCA TGCAGCTCTT TGGTGGAAGG TTCATTTTTG ATGAGTACAC TCCCACCAAT
    TTTGACACTT TCCCAGCAGC CATCATGACA GTGTTTCAAA TTCTGACTGG TGAGGACTGG
    AATGAAGTCA TGTACAATGG CATCCGGTCT CAAGGTGGCG TGAAATCTGG GATGTGGTCG
    TCTGTCTACT TCATTGTTCT CACTCTGTTT GGAAATTATA CGCTGCTTAA CGTCTTCTTG
    GCTATCGCTG TCGACAATTT GGCCAATGCC CAGGAGCTCA CAAAGGACGA AGAGGAGGAG
    GAAGAGGCGT TTAATCAGAA ACATGCCCTG CAGAAAGCCA AGGAGGTTGG GCCCATGTCC
    TCTTTCATGT CCTCAGAGAG GAGGAGGAGG CCCTACCTGT ACAGGAAGCG TGCAATTCAT
    CGGAGCCGTG CAGCCTACTC CTTCGAGGAT GCAGAGAGTC TGGCTGACGC TCGTCCATTC
    AATGCCTCTA ACTCTTTCAT GTCCAGGAGA GAGAGGAGGA GGAGGTTGAC CATGTCAGTG
    TGGGAGCAGA GGACCAGCCA GCTGCGGCGG CACAGGCAGA TGTCCAGCCG CGAGATCCTT
    TTCAGCAGCC CCAGTGAAGA ACAGGACGGA CCACCAGGCT GCCCTCTCCA CCGGAAACCT
    GGACTATCTC CATCTGGCAG CAAGCGCAGA GCCACTGAGA CCACTTCAAC CACAATCATG
    CCAGAGGGTA CCTTAGAGCC ACCCATGCCT ATGGACATGC CGGTGGATGA GCAAGCTCTG
    CCCATTTCTA TTCCTGAACC CCCTTTAGCC ATTCCAATTC CAGAGCCACC AATGCCCGTG
    GACGCATGCC TCCCTGAACC CCCAGTTACA CTGAATCTGC CCTTAACAGA GCCATCTGAA
    TCAAAGACTT CACCCGAGGG TGCTCTGGAC ATCAACAACC ATTGCCCTAG ACTAAATGGG
    AACAGGCGGC AGCGGGCTGT CCGTAAGCAC AGACCTCCTG CTATGGGGGA CATGCTGACC
    CCTGATATGG GGCCCAGGCC CCGACGGCCA CGCCACCGCG AGCTTCACAC AGACCCCAGG
    GGAAAAGAGA CTCTGCAAGG TGATGGTGTT GACGGTAAAG AACAAAAGCC AGGAAGTGAG
    GGTGATGAAA AGAGTGATGG AGAAATCAGT CCAGATGGCA GAGGGCTTGA ACAAAGGCAG
    AACAAACCAG ACAACACAGA ATCATCCACA GAGTCCAAAC CAGCAAATTC CCCCCGCAAA
    ATCCCACCTC TTGACTCTGA ACCTGCTACT CTACAGCTCA CTGACAACAC ACTGCAGCAG
    TCTTCACAGG GAACTACATG TGCCGGTGAG ACCGATAACA ATAACCACGG GTCCTTGAAT
    CAGGATAAGT CCTCCCTCAA CACCAGCATC GTGATAGAGG TCACCGACAC TCAGCCATCC
    ATGGAGCTGA CCTCCATTAC GGGTAATAGT AAAGATGTGG AGGCCTCAAA GCCAGAGAAA
    GAAGAAGAAA CCCCAGATGC ACCCAAAGGG CCTCCACCTG TCAGCATGTT CATCTTCAAA
    CCCAACAACC CGGTCCGCAG GGCCTGCCAC TACGTTGTCA ACCTTCGTTA TTTTGAGATG
    TCAATCCTTC TTGTGATCGC CGCCAGCAGT ATCGCATTGG CTGCCGAAGA CCCCGTCAAC
    ACTAATTCGG ACAGGAACAA AGTTCTGCGC TATTTTGACT ATGTATTCAC TGGGGTCTTC
    ACGTTTGAAA TGATAATCAA GATGATTGAT CAGGGGTTGG TCTTACACTA TGGCTCCTAT
    TTTCGTGACC CGTGGAACAT TCTGGATTTC ATAGTTGTGG TGGGAGCTCT GGTGGCTTTT
    GCTCTCACGA GTAACACAGG AAGAGACATT AAAACCATTA AATCGCTGCG TGTACTCCGA
    GTTCTGAGGC CTCTCAAGAC GATTAAAAGA CTTCCTAAAC TCAAGGCTGT GTTTGACTGT
    GTGGTAACAT CACTGAAGAA TGTTTTCAAC ATCCTCATCG TCTACAAGCT GTTTATGTTC
    ATATTTGCCG TCATTGCCGT GCAGCTTTTC AAAGGGAAAT TTTACTACTG TACCGACAGT
    TCCAAGGACA CTGAAAACGA GTGCAAAGGC TACTACATTG ACTATGATAA AGACAAGAAA
    AAGGAGAAAC GAGAGTGGAA GAGACGCGAT TTCCATTATG ATAACGTTGT CTGGGCTCTG
    TTGACTCTCT TCACCGTATC CACTGGCGAA GGCTGGCCGC AAGTTCTGCA ACACTCTGTG
    GATGTGACAG AGGAGGACCG GGGTCCCAGT CAGGGGAACA GGATGGAGAT GTCTATCTTC
    TATGTCATTT ATTTTGTGGT GTTCCCCTTC TTCTTCGTCA ACATATTTGT GGCCCTCATC
    ATCATCACTT TCCAAGAACA GGGTGACAAA ATGATGGAGG AGTGTAGTCT GGAGAAAAAC
    GAGAGGGCTT GTATCGACTT TGCCATCAGT GCCAAACCTT TGACTCGCTA CATGCCCCAG
    AACAGACAGA CCTTACAGTA CAGACTGTGG CAATTTGTGG TGTCGCCCTC ATTTGAGTAC
    ACAGTACTGA CAATGATCGC CCTTAACACC ATCGTTCTCA TGATGAAGCA TCATGATCCT
    CGTCTTGAAG GCTATGACGA TGTGCTCAAA TATCTCAACA CAATTTTCAC CGTTATTTTC
    TCTGTTGAGT GTGTTCTCAA GATCCTGGCT TTTGGTATAA TGAACTATTT CAGAGACACA
    TGGAATATAT TTGACTTCAT CACGGTTCTA GGAAGCATCA CAGAAATTCT GGTGGACAAA
    TTTCAGACTG GAAAAACTTT TAATATGGGC TTCCTGAAGC TATTCCGGGC AGCTCGGCTG
    ATTAAACTCC TGCGTCAGGG CTACACCATC CGTATTCTGC TCTGGACTTT TGTTCAGTCC
    TTCAAGGCTC TACCATATGT CTGTCTGCTC ATCGCCATGC TGTTTTTCAT CTATGCCATC
    ATTGGCATGC AGGTATTTGG CAACATCAAG ATAAATGAGA AAACCCAAAT CAATGAACAC
    AATAACTTCA GGACTTTCTT TGGTGCACTC ATGCTTTTGT TCAGGAGTGC AACAGGTGAA
    TCATGGCAGG AAATCATGCT ATCGTGTTTA GGAGAGAAGG AGTGTGAAAT TGACACTTCT
    GTCAACTCAA CAACAAAGAC AATAAAAAAC GACTGCGGTA CCGACTTTGC CTACTTCTAC
    TTTGTCTCCT TCATCTTCTT CAGTTCTTTT CTGATGCTGA ATCTGTTTGT GGCTGTGATC
    ATGGATAATT TTGAGTACCT GACACGTGAC TCATCCATTC TGGGTCCTCA CCACTTAGAT
    GAGTTTGTAC GGATCTGGGG AGAGTATGAC CGTGCTGCAT GTGGCCGTAT TCATTACACT
    GATATGTACG AGATGCTGAC CTGCATGTCG CCACCGCTTG GCCTGGGCAA GAAATGTCCC
    TCCAAAGTGG CGTATAAGCG GTTGGTTCTG ATGAACATGC CAGTGGATGA AGACATGTCT
    GTCCACTTCA CCTCCACACT TATGGCCCTC ATACGCACCG CACTGGACAT TAAAATTGCT
    CGAGGAGGGG AAGACAGGGG TCAGATGGAT GTTGAACTGC AGAAGGAAAT CAGTGTCATC
    TGGCCGCATC TCTCACAGAA ATCTCTTGAT CTGCTTGTAC CCATTCACAA AGCTAGTGAC
    ATGACCATTG GGAAGATCTA TGCAGCCATG ATGATCATGG ACTACTACAA GCAGAACAAA
    GCCAAGAAGC TCCGTCAGCA GCTCGAGGAA CAGAAACACG CACCAATGTT CCAACGCATG
    GATGCATCCT CGCTGCCGCA GGACATCCTC TGCAGTGCCA AGGCTTTGCC ATTCCTGACT
    CACAGTGCAG GATCTGCCCT CACTAGAGGT GGGTTTGTGG CACTGAGTCC TATCTCTCCT
    CAAGATCTCT TCATGCAGCC CATGACCCAA GACAGCATGG AGGAGAGGGA GGAATACCAA
    CACCCGTATC ACCTGCAAAG CATGGTGCCT GAGCACAAAG AATTTAGCAG TGTGATGTGC
    CCCACTAGCA TGCAGGCTGA TCCACTGCAA GTGCCAGGAA GAGGCGTCTC TATCCGAGCC
    TCTTCCATGC CTCGCCTGGC GGTAGAGCCA CAGGCCTCTT TAGAGAACAA ACTGATGAAG
    CGCTCATTCT CCACCATCGG GGATCAGTGT GTAAATGGTG ACTGGCAGGA AGAGTTCTCT
    CTGGAGAGGG TGAGTCCTGA CCGGCTGGAG AGACCCAGGC AAAGAAGCTT CAGAGGTGTC
    AGGTCAAACC TGCGGGAGCC AGTGGACAAA GAGAGAGGAC GGTCTAAAGA ACGAAGACAC
    CTGCTGTCTC CAGATGTGTC TCGCTGCAAC TCAGAGGAAA GGAGTCAGTC TCAGGAGAGA
    CGTCATTCTC CATCGCCCAG TGAGGGGAGG AAGCACACAG CTCTCAGACA GGGAAATAAT
    ACAGGCAGCC CTATTGCCTC CACGTCTGAT TCAGGGACGC CTCGAGGACG CCGCCAACTC
    CCACAGACAC CCTCTCGCCC TCGGCCTCAT ATCTCCTACT CTCCTTTAGT GTGCCGTGCC
    CATCCAGCAC CAGGGGATGA TCGGCCACGA GCTCCACCCG CAGAGGAAGG AGAGGAGCCC
    ACTGCAACCG GCCCAGGCAC CGCCATGGCC CACTCTACCC CTCACCGCTA CATCTCAGAG
    CCCTACCTGC CACTGCAGGA GGACGTCAAC AGTCCAGACT CCAGTGGTGT GGAGGAGACT
    CTCACTTTTG AGGCTGCAAT AGCAACCAGC CTGGGCCGCT CTAATACCAT TAGCTCGGCG
    CCACAGCTAC GTCACTCCTG GCAGGTGCCT AATGGACATT TCCGCAAACG ACTAGCACAA
    GCCACAGCTG GGACGTCCGG CTGTGAGCTT CTCAGTGACA CCGATGAGGA TGACCGCTGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44279
    Clone ID Related Accession (Same CDS sequence) XM_017357427.2
    Accession Version XM_017357427.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTGGAAT TGGGCACTTG TTCGGTCAGC GCTCAGACTC TCTCTGACCG TAATGCCCTG 
    GCGGTCCCGT CATTTGGTCA GGGCATATGT GGCGGGAGCC CACCTTCACG ATGTTTCCTT
    CTCCAGCCGC AGGACCCGGA GAGCTGGCTC AAGCACCGGG GAACCCTGGA GGATGGCTCC
    CGCTCCTGTT CACTCCTGGA GCCTCAGAGC CTGGCTGACA GCCTTCTGTC CCATGCATCC
    AGTTTTGATA TGCTCTACGT CCCACATGGG CGTCCAATGG GCCTTGCACC GTCTTCTCTG
    GATCTGGGCG GCTCGTGTGA GGGGGCGGCC GCGTTGGGTC TGGACCCCGA CATGCCCGTC
    AGCCCGAATA TCATTAAACG GCGCAGGGGG GGACTCATTG AACAACGGGA CATCATTAAA
    GCACACCAGG CACACAAGAT CCAGAGCACC CCACAGGCCC GACGCAAGGA ATGGGAAATG
    GCTCGCTTCG GAGACGATGT TCCCGGTCTG CTGGGAGCCG GGGATGGGGG TTCGGAGCGC
    AGTCGGAACC GGGATGCTCC GGCGGTATCC ACAGGTGGAG TTTCACCAGC CTTCAAACAG
    ACCAAAGCGC AACGAGCCCG AACCATGGCT CTGTACAACC CCATTCCTGT CCGACAGAAC
    TGCTTCACCG TCAACAGATC TCTCTTCATC TTTGGAGAGG ACAACATCGT TAGGAAGTAT
    GCCAAGAAGA TCATAGAGTG GCCTCCGTTT GAATACATGA TCCTGGCCAC CATTATTGCT
    AACTGCATCG TCCTAGCCTT GGAGCAGCAC CTGCCTGGAG AAGACAAGAC ACCCATGGCT
    AAGAGACTTG AAAAAACAGA GCCCTACTTC ATAGGAATGT TTTGCTTTGA AGCTGGAATA
    AAAATCATTG CTTTGGGATT TGTATTCCAC AAGGGCTCGT ACCTGAGGAA TGGTTGGAAC
    GTCATGGACT TTATTGTCGT ATCGAGTGGG ATTCTGGCCA CAGCAGGAGC CCACATGAAT
    ATTTCATTAG ACCTGCGCAC ACTGAGGGCT GTGAGGGTAT TACGGCCCCT CAAACTGGTC
    TCTGGCATTC CTAGTCTGCA AATAGTTCTT AAATCCATAA TGAAGGCAAT GGTGCCATTG
    CTTCAGATCG GACTGCTACT GTTTTTTGCT ATTCTAATGT TTGCCATTAT TGGATTAGAG
    TTCTACAGTG GGAAACTCCA CAAGCAATGT GAACCTGTAG GTAAAGATTC TGAGAGAGCA
    GACTCGCCTG AGAATGAATT TCCATGTGGA AGCAAACTGT GTCCTGACCA TTATAACTGC
    TCTGGCTACT GGACGGGACC CAATGATGGC ATCACTCAGT TTGATAACAT CTTATTTTCT
    TTACTCACCG TCTTCCAGTG CATCACCATG GAAGGCTGGA CGACCATCCT CTATTACACC
    AACGATGCCT TAGGCTCTAC TTGGAATTGG CTCTACTTCA TTCCTCTAAT CATAATTGGC
    TCCTTCTTTG TGTTGAATCT GGTATTAGGA GTCCTGTCAG GGGAGTTTGC TAAAGAGCGA
    GAGCGGGTAG AGAACCGGAG AGCCTTCATG AAGCTTAGGA GACAGCAGCA GATTGAGAGG
    GAGCTGAACG GATATCGGGC CTGGATCGAC CGTGCAGAGG AGGTTATGCT TGCAGAGGAG
    AACAAGAATT CAGGACCTTC TGCTTTGGAT GTGCTAAAAA GAGCTACCAC CATAAAGAGA
    AGAGGAGTGG ATGATGCTCG AAGAGCTCCA GAAGAGCAGT ATGCTGACAT CTCATCTCTA
    GGGATGCCAC TGAACAGAGC CAGCATACGC AGTGCTAAAA GAGGACCAAC ATCCTATTTC
    CGACGGAAAG AGCGCCTGCT ACGGATCTCC GTCCGCCGTA TGGTGAAGAC TCAGACGTTC
    TACTGGACTG TTCTGGGTCT GGTAGCCCTC AACACTCTCT GTGTAGCCAT CGTACATCAC
    AAACAGCCAC AGTGGTTATC TAGTCTCCTC TACTATGCAG AGTTTTTGTT TCTGGGTTTG
    TTTCTTACCG AGATGTTTCT GAAGATGTAT GGACTCGGAC CCAGACTCTA TTTTCACTCC
    TCCTTCAACT GCTTCGATTG TGGGGTTATA GTAGGCAGCA TCTTTGAGGT GTTCTGGGGC
    TTCTTCAGAC CTGGCACATC CTTTGGGATC AGCGTTCTTC GTGCCTTGCG GTTGTTGAGA
    ATTTTCAAGA TAACCAAATA CTGGGCGTCC CTGAGGAACC TGGTCGTGTC TCTGATGAAC
    TCAATGAAGT CTATTATCAG TTTGCTCTTC CTGCTTTTTC TCTTTATTGT GGTCTTTGCA
    CTTCTGGGCA TGCAGCTCTT TGGTGGAAGG TTCATTTTTG ATGAGTACAC TCCCACCAAT
    TTTGACACTT TCCCAGCAGC CATCATGACA GTGTTTCAAA TTCTGACTGG TGAGGACTGG
    AATGAAGTCA TGTACAATGG CATCCGGTCT CAAGGTGGCG TGAAATCTGG GATGTGGTCG
    TCTGTCTACT TCATTGTTCT CACTCTGTTT GGAAATTATA CGCTGCTTAA CGTCTTCTTG
    GCTATCGCTG TCGACAATTT GGCCAATGCC CAGGAGCTCA CAAAGGACGA AGAGGAGGAG
    GAAGAGGCGT TTAATCAGAA ACATGCCCTG CAGAAAGCCA AGGAGGTTGG GCCCATGTCC
    TCTTTCATGT CCTCAGAGAG GAGGAGGAGG CCCTACCTGT ACAGGAAGCG TGCAATTCAT
    CGGAGCCGTG CAGCCTACTC CTTCGAGGAT GCAGAGAGTC TGGCTGACGC TCGTCCATTC
    AATGCCTCTA ACTCTTTCAT GTCCAGGAGA GAGAGGAGGA GGAGGTTGAC CATGTCAGTG
    TGGGAGCAGA GGACCAGCCA GCTGCGGCGG CACAGGCAGA TGTCCAGCCG CGAGATCCTT
    TTCAGCAGCC CCAGTGAAGA ACAGGACGGA CCACCAGGCT GCCCTCTCCA CCGGAAACCT
    GGACTATCTC CATCTGGCAG CAAGCGCAGA GCCACTGAGA CCACTTCAAC CACAATCATG
    CCAGAGGGTA CCTTAGAGCC ACCCATGCCT ATGGACATGC CGGTGGATGA GCAAGCTCTG
    CCCATTTCTA TTCCTGAACC CCCTTTAGCC ATTCCAATTC CAGAGCCACC AATGCCCGTG
    GACGCATGCC TCCCTGAACC CCCAGTTACA CTGAATCTGC CCTTAACAGA GCCATCTGAA
    TCAAAGACTT CACCCGAGGG TGCTCTGGAC ATCAACAACC ATTGCCCTAG ACTAAATGGG
    AACAGGCGGC AGCGGGCTGT CCGTAAGCAC AGACCTCCTG CTATGGGGGA CATGCTGACC
    CCTGATATGG GGCCCAGGCC CCGACGGCCA CGCCACCGCG AGCTTCACAC AGACCCCAGG
    GGAAAAGAGA CTCTGCAAGG TGATGGTGTT GACGGTAAAG AACAAAAGCC AGGAAGTGAG
    GGTGATGAAA AGAGTGATGG AGAAATCAGT CCAGATGGCA GAGGGCTTGA ACAAAGGCAG
    AACAAACCAG ACAACACAGA ATCATCCACA GAGTCCAAAC CAGCAAATTC CCCCCGCAAA
    ATCCCACCTC TTGACTCTGA ACCTGCTACT CTACAGCTCA CTGACAACAC ACTGCAGCAG
    TCTTCACAGG GAACTACATG TGCCGGTGAG ACCGATAACA ATAACCACGG GTCCTTGAAT
    CAGGATAAGT CCTCCCTCAA CACCAGCATC GTGATAGAGG TCACCGACAC TCAGCCATCC
    ATGGAGCTGA CCTCCATTAC GGGTAATAGT AAAGATGTGG AGGCCTCAAA GCCAGAGAAA
    GAAGAAGAAA CCCCAGATGC ACCCAAAGGG CCTCCACCTG TCAGCATGTT CATCTTCAAA
    CCCAACAACC CGGTCCGCAG GGCCTGCCAC TACGTTGTCA ACCTTCGTTA TTTTGAGATG
    TCAATCCTTC TTGTGATCGC CGCCAGCAGT ATCGCATTGG CTGCCGAAGA CCCCGTCAAC
    ACTAATTCGG ACAGGAACAA AGTTCTGCGC TATTTTGACT ATGTATTCAC TGGGGTCTTC
    ACGTTTGAAA TGATAATCAA GATGATTGAT CAGGGGTTGG TCTTACACTA TGGCTCCTAT
    TTTCGTGACC CGTGGAACAT TCTGGATTTC ATAGTTGTGG TGGGAGCTCT GGTGGCTTTT
    GCTCTCACGA GTAACACAGG AAGAGACATT AAAACCATTA AATCGCTGCG TGTACTCCGA
    GTTCTGAGGC CTCTCAAGAC GATTAAAAGA CTTCCTAAAC TCAAGGCTGT GTTTGACTGT
    GTGGTAACAT CACTGAAGAA TGTTTTCAAC ATCCTCATCG TCTACAAGCT GTTTATGTTC
    ATATTTGCCG TCATTGCCGT GCAGCTTTTC AAAGGGAAAT TTTACTACTG TACCGACAGT
    TCCAAGGACA CTGAAAACGA GTGCAAAGGC TACTACATTG ACTATGATAA AGACAAGAAA
    AAGGAGAAAC GAGAGTGGAA GAGACGCGAT TTCCATTATG ATAACGTTGT CTGGGCTCTG
    TTGACTCTCT TCACCGTATC CACTGGCGAA GGCTGGCCGC AAGTTCTGCA ACACTCTGTG
    GATGTGACAG AGGAGGACCG GGGTCCCAGT CAGGGGAACA GGATGGAGAT GTCTATCTTC
    TATGTCATTT ATTTTGTGGT GTTCCCCTTC TTCTTCGTCA ACATATTTGT GGCCCTCATC
    ATCATCACTT TCCAAGAACA GGGTGACAAA ATGATGGAGG AGTGTAGTCT GGAGAAAAAC
    GAGAGGGCTT GTATCGACTT TGCCATCAGT GCCAAACCTT TGACTCGCTA CATGCCCCAG
    AACAGACAGA CCTTACAGTA CAGACTGTGG CAATTTGTGG TGTCGCCCTC ATTTGAGTAC
    ACAGTACTGA CAATGATCGC CCTTAACACC ATCGTTCTCA TGATGAAGCA TCATGATCCT
    CGTCTTGAAG GCTATGACGA TGTGCTCAAA TATCTCAACA CAATTTTCAC CGTTATTTTC
    TCTGTTGAGT GTGTTCTCAA GATCCTGGCT TTTGGTATAA TGAACTATTT CAGAGACACA
    TGGAATATAT TTGACTTCAT CACGGTTCTA GGAAGCATCA CAGAAATTCT GGTGGACAAA
    TTTCAGACTG GAAAAACTTT TAATATGGGC TTCCTGAAGC TATTCCGGGC AGCTCGGCTG
    ATTAAACTCC TGCGTCAGGG CTACACCATC CGTATTCTGC TCTGGACTTT TGTTCAGTCC
    TTCAAGGCTC TACCATATGT CTGTCTGCTC ATCGCCATGC TGTTTTTCAT CTATGCCATC
    ATTGGCATGC AGGTATTTGG CAACATCAAG ATAAATGAGA AAACCCAAAT CAATGAACAC
    AATAACTTCA GGACTTTCTT TGGTGCACTC ATGCTTTTGT TCAGGAGTGC AACAGGTGAA
    TCATGGCAGG AAATCATGCT ATCGTGTTTA GGAGAGAAGG AGTGTGAAAT TGACACTTCT
    GTCAACTCAA CAACAAAGAC AATAAAAAAC GACTGCGGTA CCGACTTTGC CTACTTCTAC
    TTTGTCTCCT TCATCTTCTT CAGTTCTTTT CTGATGCTGA ATCTGTTTGT GGCTGTGATC
    ATGGATAATT TTGAGTACCT GACACGTGAC TCATCCATTC TGGGTCCTCA CCACTTAGAT
    GAGTTTGTAC GGATCTGGGG AGAGTATGAC CGTGCTGCAT GTGGAAAACT ACACTATAAG
    GAGATGTACA AAGTAGTACG CACAATATCA CCTCCTCTGG GCTTTGGAAA GAATTGCCCC
    TACAGGGTGG CATGCAAGCG GTTGGTTCTG ATGAACATGC CAGTGGATGA AGACATGTCT
    GTCCACTTCA CCTCCACACT TATGGCCCTC ATACGCACCG CACTGGACAT TAAAATTGCT
    CGAGGAGGGG AAGACAGGGG TCAGATGGAT GTTGAACTGC AGAAGGAAAT CAGTGTCATC
    TGGCCGCATC TCTCACAGAA ATCTCTTGAT CTGCTTGTAC CCATTCACAA AGCTAGTGAC
    ATGACCATTG GGAAGATCTA TGCAGCCATG ATGATCATGG ACTACTACAA GCAGAACAAA
    GCCAAGAAGC TCCGTCAGCA GCTCGAGGAA CAGAAACACG CACCAATGTT CCAACGCATG
    GATGCATCCT CGCTGCCGCA GGACATCCTC TGCAGTGCCA AGGCTTTGCC ATTCCTGACT
    CACAGTGCAG GATCTGCCCT CACTAGAGGT GGGTTTGTGG CACTGAGTCC TATCTCTCCT
    CAAGATCTCT TCATGCAGCC CATGACCCAA GACAGCATGG AGGAGAGGGA GGAATACCAA
    CACCCGTATC ACCTGCAAAG CATGGTGCCT GAGCACAAAG AATTTAGCAG TGTGATGTGC
    CCCACTAGCA TGCAGGCTGA TCCACTGCAA GTGCCAGGAA GAGGCGTCTC TATCCGAGCC
    TCTTCCATGC CTCGCCTGGC GGTAGAGCCA CAGGCCTCTT TAGAGAACAA ACTGATGAAG
    CGCTCATTCT CCACCATCGG GGATCAGTGT GTAAATGGTG ACTGGCAGGA AGAGTTCTCT
    CTGGAGAGGG TGAGTCCTGA CCGGCTGGAG AGACCCAGGC AAAGAAGCTT CAGAGGTGTC
    AGGTCAAACC TGCGGGAGCC AGTGGACAAA GAGAGAGGAC GGTCTAAAGA ACGAAGACAC
    CTGCTGTCTC CAGATGTGTC TCGCTGCAAC TCAGAGGAAA GGAGTCAGTC TCAGGAGAGA
    CGTCATTCTC CATCGCCCAG TGAGGGGAGG AAGCACACAG CTCTCAGACA GGGAAATAAT
    ACAGGCAGCC CTATTGCCTC CACGTCTGAT TCAGGGACGC CTCGAGGACG CCGCCAACTC
    CCACAGACAC CCTCTCGCCC TCGGCCTCAT ATCTCCTACT CTCCTTTAGT GTGCCGTGCC
    CATCCAGCAC CAGGGGATGA TCGGCCACGA GCTCCACCCG CAGAGGAAGG AGAGGAGCCC
    ACTGCAACCG GCCCAGGCAC CGCCATGGCC CACTCTACCC CTCACCGCTA CATCTCAGAG
    CCCTACCTGC CACTGCAGGA GGACGTCAAC AGTCCAGACT CCAGTGGTGT GGAGGAGACT
    CTCACTTTTG AGGCTGCAAT AGCAACCAGC CTGGGCCGCT CTAATACCAT TAGCTCGGCG
    CCACAGCTAC GTCACTCCTG GCAGGTGCCT AATGGACATT TCCGCAAACG ACTAGCACAA
    GCCACAGCTG GGACGTCCGG CTGTGAGCTT CTCAGTGACA CCGATGAGGA TGACCGCTGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212916.2
    CDS388..7710
    Translation

    Target ORF information:

    RefSeq Version XM_017357427.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357427.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTTGGAAT TGGGCACTTG TTCGGTCAGC GCTCAGACTC TCTCTGACCG TAATGCCCTG 
    GCGGTCCCGT CATTTGGTCA GGGCATATGT GGCGGGAGCC CACCTTCACG ATGTTTCCTT
    CTCCAGCCGC AGGACCCGGA GAGCTGGCTC AAGCACCGGG GAACCCTGGA GGATGGCTCC
    CGCTCCTGTT CACTCCTGGA GCCTCAGAGC CTGGCTGACA GCCTTCTGTC CCATGCATCC
    AGTTTTGATA TGCTCTACGT CCCACATGGG CGTCCAATGG GCCTTGCACC GTCTTCTCTG
    GATCTGGGCG GCTCGTGTGA GGGGGCGGCC GCGTTGGGTC TGGACCCCGA CATGCCCGTC
    AGCCCGAATA TCATTAAACG GCGCAGGGGG GGACTCATTG AACAACGGGA CATCATTAAA
    GCACACCAGG CACACAAGAT CCAGAGCACC CCACAGGCCC GACGCAAGGA ATGGGAAATG
    GCTCGCTTCG GAGACGATGT TCCCGGTCTG CTGGGAGCCG GGGATGGGGG TTCGGAGCGC
    AGTCGGAACC GGGATGCTCC GGCGGTATCC ACAGGTGGAG TTTCACCAGC CTTCAAACAG
    ACCAAAGCGC AACGAGCCCG AACCATGGCT CTGTACAACC CCATTCCTGT CCGACAGAAC
    TGCTTCACCG TCAACAGATC TCTCTTCATC TTTGGAGAGG ACAACATCGT TAGGAAGTAT
    GCCAAGAAGA TCATAGAGTG GCCTCCGTTT GAATACATGA TCCTGGCCAC CATTATTGCT
    AACTGCATCG TCCTAGCCTT GGAGCAGCAC CTGCCTGGAG AAGACAAGAC ACCCATGGCT
    AAGAGACTTG AAAAAACAGA GCCCTACTTC ATAGGAATGT TTTGCTTTGA AGCTGGAATA
    AAAATCATTG CTTTGGGATT TGTATTCCAC AAGGGCTCGT ACCTGAGGAA TGGTTGGAAC
    GTCATGGACT TTATTGTCGT ATCGAGTGGG ATTCTGGCCA CAGCAGGAGC CCACATGAAT
    ATTTCATTAG ACCTGCGCAC ACTGAGGGCT GTGAGGGTAT TACGGCCCCT CAAACTGGTC
    TCTGGCATTC CTAGTCTGCA AATAGTTCTT AAATCCATAA TGAAGGCAAT GGTGCCATTG
    CTTCAGATCG GACTGCTACT GTTTTTTGCT ATTCTAATGT TTGCCATTAT TGGATTAGAG
    TTCTACAGTG GGAAACTCCA CAAGCAATGT GAACCTGTAG GTAAAGATTC TGAGAGAGCA
    GACTCGCCTG AGAATGAATT TCCATGTGGA AGCAAACTGT GTCCTGACCA TTATAACTGC
    TCTGGCTACT GGACGGGACC CAATGATGGC ATCACTCAGT TTGATAACAT CTTATTTTCT
    TTACTCACCG TCTTCCAGTG CATCACCATG GAAGGCTGGA CGACCATCCT CTATTACACC
    AACGATGCCT TAGGCTCTAC TTGGAATTGG CTCTACTTCA TTCCTCTAAT CATAATTGGC
    TCCTTCTTTG TGTTGAATCT GGTATTAGGA GTCCTGTCAG GGGAGTTTGC TAAAGAGCGA
    GAGCGGGTAG AGAACCGGAG AGCCTTCATG AAGCTTAGGA GACAGCAGCA GATTGAGAGG
    GAGCTGAACG GATATCGGGC CTGGATCGAC CGTGCAGAGG AGGTTATGCT TGCAGAGGAG
    AACAAGAATT CAGGACCTTC TGCTTTGGAT GTGCTAAAAA GAGCTACCAC CATAAAGAGA
    AGAGGAGTGG ATGATGCTCG AAGAGCTCCA GAAGAGCAGT ATGCTGACAT CTCATCTCTA
    GGGATGCCAC TGAACAGAGC CAGCATACGC AGTGCTAAAA GAGGACCAAC ATCCTATTTC
    CGACGGAAAG AGCGCCTGCT ACGGATCTCC GTCCGCCGTA TGGTGAAGAC TCAGACGTTC
    TACTGGACTG TTCTGGGTCT GGTAGCCCTC AACACTCTCT GTGTAGCCAT CGTACATCAC
    AAACAGCCAC AGTGGTTATC TAGTCTCCTC TACTATGCAG AGTTTTTGTT TCTGGGTTTG
    TTTCTTACCG AGATGTTTCT GAAGATGTAT GGACTCGGAC CCAGACTCTA TTTTCACTCC
    TCCTTCAACT GCTTCGATTG TGGGGTTATA GTAGGCAGCA TCTTTGAGGT GTTCTGGGGC
    TTCTTCAGAC CTGGCACATC CTTTGGGATC AGCGTTCTTC GTGCCTTGCG GTTGTTGAGA
    ATTTTCAAGA TAACCAAATA CTGGGCGTCC CTGAGGAACC TGGTCGTGTC TCTGATGAAC
    TCAATGAAGT CTATTATCAG TTTGCTCTTC CTGCTTTTTC TCTTTATTGT GGTCTTTGCA
    CTTCTGGGCA TGCAGCTCTT TGGTGGAAGG TTCATTTTTG ATGAGTACAC TCCCACCAAT
    TTTGACACTT TCCCAGCAGC CATCATGACA GTGTTTCAAA TTCTGACTGG TGAGGACTGG
    AATGAAGTCA TGTACAATGG CATCCGGTCT CAAGGTGGCG TGAAATCTGG GATGTGGTCG
    TCTGTCTACT TCATTGTTCT CACTCTGTTT GGAAATTATA CGCTGCTTAA CGTCTTCTTG
    GCTATCGCTG TCGACAATTT GGCCAATGCC CAGGAGCTCA CAAAGGACGA AGAGGAGGAG
    GAAGAGGCGT TTAATCAGAA ACATGCCCTG CAGAAAGCCA AGGAGGTTGG GCCCATGTCC
    TCTTTCATGT CCTCAGAGAG GAGGAGGAGG CCCTACCTGT ACAGGAAGCG TGCAATTCAT
    CGGAGCCGTG CAGCCTACTC CTTCGAGGAT GCAGAGAGTC TGGCTGACGC TCGTCCATTC
    AATGCCTCTA ACTCTTTCAT GTCCAGGAGA GAGAGGAGGA GGAGGTTGAC CATGTCAGTG
    TGGGAGCAGA GGACCAGCCA GCTGCGGCGG CACAGGCAGA TGTCCAGCCG CGAGATCCTT
    TTCAGCAGCC CCAGTGAAGA ACAGGACGGA CCACCAGGCT GCCCTCTCCA CCGGAAACCT
    GGACTATCTC CATCTGGCAG CAAGCGCAGA GCCACTGAGA CCACTTCAAC CACAATCATG
    CCAGAGGGTA CCTTAGAGCC ACCCATGCCT ATGGACATGC CGGTGGATGA GCAAGCTCTG
    CCCATTTCTA TTCCTGAACC CCCTTTAGCC ATTCCAATTC CAGAGCCACC AATGCCCGTG
    GACGCATGCC TCCCTGAACC CCCAGTTACA CTGAATCTGC CCTTAACAGA GCCATCTGAA
    TCAAAGACTT CACCCGAGGG TGCTCTGGAC ATCAACAACC ATTGCCCTAG ACTAAATGGG
    AACAGGCGGC AGCGGGCTGT CCGTAAGCAC AGACCTCCTG CTATGGGGGA CATGCTGACC
    CCTGATATGG GGCCCAGGCC CCGACGGCCA CGCCACCGCG AGCTTCACAC AGACCCCAGG
    GGAAAAGAGA CTCTGCAAGG TGATGGTGTT GACGGTAAAG AACAAAAGCC AGGAAGTGAG
    GGTGATGAAA AGAGTGATGG AGAAATCAGT CCAGATGGCA GAGGGCTTGA ACAAAGGCAG
    AACAAACCAG ACAACACAGA ATCATCCACA GAGTCCAAAC CAGCAAATTC CCCCCGCAAA
    ATCCCACCTC TTGACTCTGA ACCTGCTACT CTACAGCTCA CTGACAACAC ACTGCAGCAG
    TCTTCACAGG GAACTACATG TGCCGGTGAG ACCGATAACA ATAACCACGG GTCCTTGAAT
    CAGGATAAGT CCTCCCTCAA CACCAGCATC GTGATAGAGG TCACCGACAC TCAGCCATCC
    ATGGAGCTGA CCTCCATTAC GGGTAATAGT AAAGATGTGG AGGCCTCAAA GCCAGAGAAA
    GAAGAAGAAA CCCCAGATGC ACCCAAAGGG CCTCCACCTG TCAGCATGTT CATCTTCAAA
    CCCAACAACC CGGTCCGCAG GGCCTGCCAC TACGTTGTCA ACCTTCGTTA TTTTGAGATG
    TCAATCCTTC TTGTGATCGC CGCCAGCAGT ATCGCATTGG CTGCCGAAGA CCCCGTCAAC
    ACTAATTCGG ACAGGAACAA AGTTCTGCGC TATTTTGACT ATGTATTCAC TGGGGTCTTC
    ACGTTTGAAA TGATAATCAA GATGATTGAT CAGGGGTTGG TCTTACACTA TGGCTCCTAT
    TTTCGTGACC CGTGGAACAT TCTGGATTTC ATAGTTGTGG TGGGAGCTCT GGTGGCTTTT
    GCTCTCACGA GTAACACAGG AAGAGACATT AAAACCATTA AATCGCTGCG TGTACTCCGA
    GTTCTGAGGC CTCTCAAGAC GATTAAAAGA CTTCCTAAAC TCAAGGCTGT GTTTGACTGT
    GTGGTAACAT CACTGAAGAA TGTTTTCAAC ATCCTCATCG TCTACAAGCT GTTTATGTTC
    ATATTTGCCG TCATTGCCGT GCAGCTTTTC AAAGGGAAAT TTTACTACTG TACCGACAGT
    TCCAAGGACA CTGAAAACGA GTGCAAAGGC TACTACATTG ACTATGATAA AGACAAGAAA
    AAGGAGAAAC GAGAGTGGAA GAGACGCGAT TTCCATTATG ATAACGTTGT CTGGGCTCTG
    TTGACTCTCT TCACCGTATC CACTGGCGAA GGCTGGCCGC AAGTTCTGCA ACACTCTGTG
    GATGTGACAG AGGAGGACCG GGGTCCCAGT CAGGGGAACA GGATGGAGAT GTCTATCTTC
    TATGTCATTT ATTTTGTGGT GTTCCCCTTC TTCTTCGTCA ACATATTTGT GGCCCTCATC
    ATCATCACTT TCCAAGAACA GGGTGACAAA ATGATGGAGG AGTGTAGTCT GGAGAAAAAC
    GAGAGGGCTT GTATCGACTT TGCCATCAGT GCCAAACCTT TGACTCGCTA CATGCCCCAG
    AACAGACAGA CCTTACAGTA CAGACTGTGG CAATTTGTGG TGTCGCCCTC ATTTGAGTAC
    ACAGTACTGA CAATGATCGC CCTTAACACC ATCGTTCTCA TGATGAAGCA TCATGATCCT
    CGTCTTGAAG GCTATGACGA TGTGCTCAAA TATCTCAACA CAATTTTCAC CGTTATTTTC
    TCTGTTGAGT GTGTTCTCAA GATCCTGGCT TTTGGTATAA TGAACTATTT CAGAGACACA
    TGGAATATAT TTGACTTCAT CACGGTTCTA GGAAGCATCA CAGAAATTCT GGTGGACAAA
    TTTCAGACTG GAAAAACTTT TAATATGGGC TTCCTGAAGC TATTCCGGGC AGCTCGGCTG
    ATTAAACTCC TGCGTCAGGG CTACACCATC CGTATTCTGC TCTGGACTTT TGTTCAGTCC
    TTCAAGGCTC TACCATATGT CTGTCTGCTC ATCGCCATGC TGTTTTTCAT CTATGCCATC
    ATTGGCATGC AGGTATTTGG CAACATCAAG ATAAATGAGA AAACCCAAAT CAATGAACAC
    AATAACTTCA GGACTTTCTT TGGTGCACTC ATGCTTTTGT TCAGGAGTGC AACAGGTGAA
    TCATGGCAGG AAATCATGCT ATCGTGTTTA GGAGAGAAGG AGTGTGAAAT TGACACTTCT
    GTCAACTCAA CAACAAAGAC AATAAAAAAC GACTGCGGTA CCGACTTTGC CTACTTCTAC
    TTTGTCTCCT TCATCTTCTT CAGTTCTTTT CTGATGCTGA ATCTGTTTGT GGCTGTGATC
    ATGGATAATT TTGAGTACCT GACACGTGAC TCATCCATTC TGGGTCCTCA CCACTTAGAT
    GAGTTTGTAC GGATCTGGGG AGAGTATGAC CGTGCTGCAT GTGGAAAACT ACACTATAAG
    GAGATGTACA AAGTAGTACG CACAATATCA CCTCCTCTGG GCTTTGGAAA GAATTGCCCC
    TACAGGGTGG CATGCAAGCG GTTGGTTCTG ATGAACATGC CAGTGGATGA AGACATGTCT
    GTCCACTTCA CCTCCACACT TATGGCCCTC ATACGCACCG CACTGGACAT TAAAATTGCT
    CGAGGAGGGG AAGACAGGGG TCAGATGGAT GTTGAACTGC AGAAGGAAAT CAGTGTCATC
    TGGCCGCATC TCTCACAGAA ATCTCTTGAT CTGCTTGTAC CCATTCACAA AGCTAGTGAC
    ATGACCATTG GGAAGATCTA TGCAGCCATG ATGATCATGG ACTACTACAA GCAGAACAAA
    GCCAAGAAGC TCCGTCAGCA GCTCGAGGAA CAGAAACACG CACCAATGTT CCAACGCATG
    GATGCATCCT CGCTGCCGCA GGACATCCTC TGCAGTGCCA AGGCTTTGCC ATTCCTGACT
    CACAGTGCAG GATCTGCCCT CACTAGAGGT GGGTTTGTGG CACTGAGTCC TATCTCTCCT
    CAAGATCTCT TCATGCAGCC CATGACCCAA GACAGCATGG AGGAGAGGGA GGAATACCAA
    CACCCGTATC ACCTGCAAAG CATGGTGCCT GAGCACAAAG AATTTAGCAG TGTGATGTGC
    CCCACTAGCA TGCAGGCTGA TCCACTGCAA GTGCCAGGAA GAGGCGTCTC TATCCGAGCC
    TCTTCCATGC CTCGCCTGGC GGTAGAGCCA CAGGCCTCTT TAGAGAACAA ACTGATGAAG
    CGCTCATTCT CCACCATCGG GGATCAGTGT GTAAATGGTG ACTGGCAGGA AGAGTTCTCT
    CTGGAGAGGG TGAGTCCTGA CCGGCTGGAG AGACCCAGGC AAAGAAGCTT CAGAGGTGTC
    AGGTCAAACC TGCGGGAGCC AGTGGACAAA GAGAGAGGAC GGTCTAAAGA ACGAAGACAC
    CTGCTGTCTC CAGATGTGTC TCGCTGCAAC TCAGAGGAAA GGAGTCAGTC TCAGGAGAGA
    CGTCATTCTC CATCGCCCAG TGAGGGGAGG AAGCACACAG CTCTCAGACA GGGAAATAAT
    ACAGGCAGCC CTATTGCCTC CACGTCTGAT TCAGGGACGC CTCGAGGACG CCGCCAACTC
    CCACAGACAC CCTCTCGCCC TCGGCCTCAT ATCTCCTACT CTCCTTTAGT GTGCCGTGCC
    CATCCAGCAC CAGGGGATGA TCGGCCACGA GCTCCACCCG CAGAGGAAGG AGAGGAGCCC
    ACTGCAACCG GCCCAGGCAC CGCCATGGCC CACTCTACCC CTCACCGCTA CATCTCAGAG
    CCCTACCTGC CACTGCAGGA GGACGTCAAC AGTCCAGACT CCAGTGGTGT GGAGGAGACT
    CTCACTTTTG AGGCTGCAAT AGCAACCAGC CTGGGCCGCT CTAATACCAT TAGCTCGGCG
    CCACAGCTAC GTCACTCCTG GCAGGTGCCT AATGGACATT TCCGCAAACG ACTAGCACAA
    GCCACAGCTG GGACGTCCGG CTGTGAGCTT CTCAGTGACA CCGATGAGGA TGACCGCTGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44280
    Clone ID Related Accession (Same CDS sequence) XM_021478387.1
    Accession Version XM_021478387.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTTGGAAT TGGGCACTTG TTCGGTCAGC GCTCAGACTC TCTCTGACCG TAATGCCCTG 
    GCGGTCCCGT CATTTGGTCA GGGCATATGT GGCGGGAGCC CACCTTCACG ATGTTTCCTT
    CTCCAGCCGC AGGACCCGGA GAGCTGGCTC AAGCACCGGG GAACCCTGGA GGATGGCTCC
    CGCTCCTGTT CACTCCTGGA GCCTCAGAGC CTGGCTGACA GCCTTCTGTC CCATGCATCC
    AGTTTTGATA TGCTCTACGT CCCACATGGG CGTCCAATGG GCCTTGCACC GTCTTCTCTG
    GATCTGGGCG GCTCGTGTGA GGGGGCGGCC GCGTTGGGTC TGGACCCCGA CATGCCCGTC
    AGCCCGAATA TCATTAAACG GCGCAGGGGG GGACTCATTG AACAACGGGA CATCATTAAA
    GCACACCAGG CACACAAGAT CCAGAGCACC CCACAGGCCC GACGCAAGGA ATGGGAAATG
    GCTCGCTTCG GAGACGATGT TCCCGGTCTG CTGGGAGCCG GGGATGGGGG TTCGGAGCGC
    AGTCGGAACC GGGATGCTCC GGCGGTATCC ACAGGTGGAG TTTCACCAGC CTTCAAACAG
    ACCAAAGCGC AACGAGCCCG AACCATGGCT CTGTACAACC CCATTCCTGT CCGACAGAAC
    TGCTTCACCG TCAACAGATC TCTCTTCATC TTTGGAGAGG ACAACATCGT TAGGAAGTAT
    GCCAAGAAGA TCATAGAGTG GCCTCCGTTT GAATACATGA TCCTGGCCAC CATTATTGCT
    AACTGCATCG TCCTAGCCTT GGAGCAGCAC CTGCCTGGAG AAGACAAGAC ACCCATGGCT
    AAGAGACTTG AAAAAACAGA GCCCTACTTC ATAGGAATGT TTTGCTTTGA AGCTGGAATA
    AAAATCATTG CTTTGGGATT TGTATTCCAC AAGGGCTCGT ACCTGAGGAA TGGTTGGAAC
    GTCATGGACT TTATTGTCGT ATCGAGTGGG ATTCTGGCCA CAGCAGGAGC CCACATGAAT
    ATTTCATTAG ACCTGCGCAC ACTGAGGGCT GTGAGGGTAT TACGGCCCCT CAAACTGGTC
    TCTGGCATTC CTAGTCTGCA AATAGTTCTT AAATCCATAA TGAAGGCAAT GGTGCCATTG
    CTTCAGATCG GACTGCTACT GTTTTTTGCT ATTCTAATGT TTGCCATTAT TGGATTAGAG
    TTCTACAGTG GGAAACTCCA CAAGCAATGT GAACCTGTAG GTAAAGATTC TGAGAGAGCA
    GACTCGCCTG AGAATGAATT TCCATGTGGA AGCAAACTGT GTCCTGACCA TTATAACTGC
    TCTGGCTACT GGACGGGACC CAATGATGGC ATCACTCAGT TTGATAACAT CTTATTTTCT
    TTACTCACCG TCTTCCAGTG CATCACCATG GAAGGCTGGA CGACCATCCT CTATTACACC
    AACGATGCCT TAGGCTCTAC TTGGAATTGG CTCTACTTCA TTCCTCTAAT CATAATTGGC
    TCCTTCTTTG TGTTGAATCT GGTATTAGGA GTCCTGTCAG GGGAGTTTGC TAAAGAGCGA
    GAGCGGGTAG AGAACCGGAG AGCCTTCATG AAGCTTAGGA GACAGCAGCA GATTGAGAGG
    GAGCTGAACG GATATCGGGC CTGGATCGAC CGTGCAGAGG AGGTTATGCT TGCAGAGGAG
    AACAAGAATT CAGGACCTTC TGCTTTGGAT GTGCTAAAAA GAGCTACCAC CATAAAGAGA
    AGAGGAGTGG ATGATGCTCG AAGAGCTCCA GAAGAGCAGT ATGCTGACAT CTCATCTCTA
    GGGATGCCAC TGAACAGAGC CAGCATACGC AGTGCTAAAA GAGGACCAAC ATCCTATTTC
    CGACGGAAAG AGCGCCTGCT ACGGATCTCC GTCCGCCGTA TGGTGAAGAC TCAGACGTTC
    TACTGGACTG TTCTGGGTCT GGTAGCCCTC AACACTCTCT GTGTAGCCAT CGTACATCAC
    AAACAGCCAC AGTGGTTATC TAGTCTCCTC TACTATGCAG AGTTTTTGTT TCTGGGTTTG
    TTTCTTACCG AGATGTTTCT GAAGATGTAT GGACTCGGAC CCAGACTCTA TTTTCACTCC
    TCCTTCAACT GCTTCGATTG TGGGGTTATA GTAGGCAGCA TCTTTGAGGT GTTCTGGGGC
    TTCTTCAGAC CTGGCACATC CTTTGGGATC AGCGTTCTTC GTGCCTTGCG GTTGTTGAGA
    ATTTTCAAGA TAACCAAATA CTGGGCGTCC CTGAGGAACC TGGTCGTGTC TCTGATGAAC
    TCAATGAAGT CTATTATCAG TTTGCTCTTC CTGCTTTTTC TCTTTATTGT GGTCTTTGCA
    CTTCTGGGCA TGCAGCTCTT TGGTGGAAGG TTCATTTTTG ATGAGTACAC TCCCACCAAT
    TTTGACACTT TCCCAGCAGC CATCATGACA GTGTTTCAAA TTCTGACTGG TGAGGACTGG
    AATGAAGTCA TGTACAATGG CATCCGGTCT CAAGGTGGCG TGAAATCTGG GATGTGGTCG
    TCTGTCTACT TCATTGTTCT CACTCTGTTT GGAAATTATA CGCTGCTTAA CGTCTTCTTG
    GCTATCGCTG TCGACAATTT GGCCAATGCC CAGGAGCTCA CAAAGGACGA AGAGGAGGAG
    GAAGAGGCGT TTAATCAGAA ACATGCCCTG CAGAAAGCCA AGGAGGTTGG GCCCATGTCC
    TCTTTCATGT CCTCAGAGAG AGAGAGGAGG AGGAGGTTGA CCATGTCAGT GTGGGAGCAG
    AGGACCAGCC AGCTGCGGCG GCACAGGCAG ATGTCCAGCC GCGAGATCCT TTTCAGCAGC
    CCCAGTGAAG AACAGGACGG ACCACCAGGC TGCCCTCTCC ACCGGAAACC TGGACTATCT
    CCATCTGGCA GCAAGCGCAG AGCCACTGAG ACCACTTCAA CCACAATCAT GCCAGAGGGT
    ACCTTAGAGC CACCCATGCC TATGGACATG CCGGTGGATG AGCAAGCTCT GCCCATTTCT
    ATTCCTGAAC CCCCTTTAGC CATTCCAATT CCAGAGCCAC CAATGCCCGT GGACGCATGC
    CTCCCTGAAC CCCCAGTTAC ACTGAATCTG CCCTTAACAG AGCCATCTGA ATCAAAGACT
    TCACCCGAGG GTGCTCTGGA CATCAACAAC CATTGCCCTA GACTAAATGG GAACAGGCGG
    CAGCGGGCTG TCCGTAAGCA CAGACCTCCT GCTATGGGGG ACATGCTGAC CCCTGATATG
    GGGCCCAGGC CCCGACGGCC ACGCCACCGC GAGCTTCACA CAGACCCCAG GGGAAAAGAG
    ACTCTGCAAG GTGATGGTGT TGACGGTAAA GAACAAAAGC CAGGAAGTGA GGGTGATGAA
    AAGAGTGATG GAGAAATCAG TCCAGATGGC AGAGGGCTTG AACAAAGGCA GAACAAACCA
    GACAACACAG AATCATCCAC AGAGTCCAAA CCAGCAAATT CCCCCCGCAA AATCCCACCT
    CTTGACTCTG AACCTGCTAC TCTACAGCTC ACTGACAACA CACTGCAGCA GTCTTCACAG
    GGAACTACAT GTGCCGGTGA GACCGATAAC AATAACCACG GGTCCTTGAA TCAGGATAAG
    TCCTCCCTCA ACACCAGCAT CGTGATAGAG GTCACCGACA CTCAGCCATC CATGGAGCTG
    ACCTCCATTA CGGGTAATAG TAAAGATGTG GAGGCCTCAA AGCCAGAGAA AGAAGAAGAA
    ACCCCAGATG CACCCAAAGG GCCTCCACCT GTCAGCATGT TCATCTTCAA ACCCAACAAC
    CCGGTCCGCA GGGCCTGCCA CTACGTTGTC AACCTTCGTT ATTTTGAGAT GTCAATCCTT
    CTTGTGATCG CCGCCAGCAG TATCGCATTG GCTGCCGAAG ACCCCGTCAA CACTAATTCG
    GACAGGAACA AAGTTCTGCG CTATTTTGAC TATGTATTCA CTGGGGTCTT CACGTTTGAA
    ATGATAATCA AGATGATTGA TCAGGGGTTG GTCTTACACT ATGGCTCCTA TTTTCGTGAC
    CCGTGGAACA TTCTGGATTT CATAGTTGTG GTGGGAGCTC TGGTGGCTTT TGCTCTCACG
    AATGTGATGG GGAGTAACAC AGGAAGAGAC ATTAAAACCA TTAAATCGCT GCGTGTACTC
    CGAGTTCTGA GGCCTCTCAA GACGATTAAA AGACTTCCTA AACTCAAGGC TGTGTTTGAC
    TGTGTGGTAA CATCACTGAA GAATGTTTTC AACATCCTCA TCGTCTACAA GCTGTTTATG
    TTCATATTTG CCGTCATTGC CGTGCAGCTT TTCAAAGGGA AATTTTACTA CTGTACCGAC
    AGTTCCAAGG ACACTGAAAA CGAGTGCAAA GGCTACTACA TTGACTATGA TAAAGACAAG
    AAAAAGGAGA AACGAGAGTG GAAGAGACGC GATTTCCATT ATGATAACGT TGTCTGGGCT
    CTGTTGACTC TCTTCACCGT ATCCACTGGC GAAGGCTGGC CGCAAGTTCT GCAACACTCT
    GTGGATGTGA CAGAGGAGGA CCGGGGTCCC AGTCAGGGGA ACAGGATGGA GATGTCTATC
    TTCTATGTCA TTTATTTTGT GGTGTTCCCC TTCTTCTTCG TCAACATATT TGTGGCCCTC
    ATCATCATCA CTTTCCAAGA ACAGGGTGAC AAAATGATGG AGGAGTGTAG TCTGGAGAAA
    AACGAGAGGG CTTGTATCGA CTTTGCCATC AGTGCCAAAC CTTTGACTCG CTACATGCCC
    CAGAACAGAC AGACCTTACA GTACAGACTG TGGCAATTTG TGGTGTCGCC CTCATTTGAG
    TACACAGTAC TGACAATGAT CGCCCTTAAC ACCATCGTTC TCATGATGAA GCATCATGAT
    CCTCGTCTTG AAGGCTATGA CGATGTGCTC AAATATCTCA ACACAATTTT CACCGTTATT
    TTCTCTGTTG AGTGTGTTCT CAAGATCCTG GCTTTTGGTA TAATGAACTA TTTCAGAGAC
    ACATGGAATA TATTTGACTT CATCACGGTT CTAGGAAGCA TCACAGAAAT TCTGGTGGAC
    AAATTTCAGA CTGGAAAAAC TTTTAATATG GGCTTCCTGA AGCTATTCCG GGCAGCTCGG
    CTGATTAAAC TCCTGCGTCA GGGCTACACC ATCCGTATTC TGCTCTGGAC TTTTGTTCAG
    TCCTTCAAGG CTCTACCATA TGTCTGTCTG CTCATCGCCA TGCTGTTTTT CATCTATGCC
    ATCATTGGCA TGCAGGTATT TGGCAACATC AAGATAAATG AGAAAACCCA AATCAATGAA
    CACAATAACT TCAGGACTTT CTTTGGTGCA CTCATGCTTT TGTTCAGGAG TGCAACAGGT
    GAATCATGGC AGGAAATCAT GCTATCGTGT TTAGGAGAGA AGGAGTGTGA AATTGACACT
    TCTGTCAACT CAACAACAAA GACAATAAAA AACGACTGCG GTACCGACTT TGCCTACTTC
    TACTTTGTCT CCTTCATCTT CTTCAGTTCT TTTCTGATGC TGAATCTGTT TGTGGCTGTG
    ATCATGGATA ATTTTGAGTA CCTGACACGT GACTCATCCA TTCTGGGTCC TCACCACTTA
    GATGAGTTTG TACGGATCTG GGGAGAGTAT GACCGTGCTG CATGTGGCCG TATTCATTAC
    ACTGATATGT ACGAGATGCT GACCTGCATG TCGCCACCGC TTGGCCTGGG CAAGAAATGT
    CCCTCCAAAG TGGCGTATAA GCGGTTGGTT CTGATGAACA TGCCAGTGGA TGAAGACATG
    TCTGTCCACT TCACCTCCAC ACTTATGGCC CTCATACGCA CCGCACTGGA CATTAAAATT
    GCTCGAGGAG GGGAAGACAG GGGTCAGATG GATGTTGAAC TGCAGAAGGA AATCAGTGTC
    ATCTGGCCGC ATCTCTCACA GAAATCTCTT GATCTGCTTG TACCCATTCA CAAAGCTAGT
    GACATGACCA TTGGGAAGAT CTATGCAGCC ATGATGATCA TGGACTACTA CAAGCAGAAC
    AAAGCCAAGA AGCTCCGTCA GCAGCTCGAG GAACAGAAAC ACGCACCAAT GTTCCAACGC
    ATGGATGCAT CCTCGCTGCC GCAGGACATC CTCTGCAGTG CCAAGGCTTT GCCATTCCTG
    ACTCACAGTG CAGGATCTGC CCTCACTAGA GGTGGGTTTG TGGCACTGAG TCCTATCTCT
    CCTCAAGATC TCTTCATGCA GCCCATGACC CAAGACAGCA TGGAGGAGAG GGAGGAATAC
    CAACACCCGT ATCACCTGCA AAGCATGGTG CCTGAGCACA AAGAATTTAG CAGTGTGATG
    TGCCCCACTA GCATGCAGGC TGATCCACTG CAAGTGCCAG GAAGAGGCGT CTCTATCCGA
    GCCTCTTCCA TGCCTCGCCT GGCGGTAGAG CCACAGGCCT CTTTAGAGAA CAAACTGATG
    AAGCGCTCAT TCTCCACCAT CGGGGATCAG TGTGTAAATG GTGACTGGCA GGAAGAGTTC
    TCTCTGGAGA GGGTGAGTCC TGACCGGCTG GAGAGACCCA GGCAAAGAAG CTTCAGAGGT
    GTCAGGTCAA ACCTGCGGGA GCCAGTGGAC AAAGAGAGAG GACGGTCTAA AGAACGAAGA
    CACCTGCTGT CTCCAGATGT GTCTCGCTGC AACTCAGAGG AAAGGAGTCA GTCTCAGGAG
    AGACGTCATT CTCCATCGCC CAGTGAGGGG AGGAAGCACA CAGCTCTCAG ACAGGGAAAT
    AATACAGGCA GCCCTATTGC CTCCACGTCT GATTCAGGGA CGCCTCGAGG ACGCCGCCAA
    CTCCCACAGA CACCCTCTCG CCCTCGGCCT CATATCTCCT ACTCTCCTTT AGTGTGCCGT
    GCCCATCCAG CACCAGGGGA TGATCGGCCA CGAGCTCCAC CCGCAGAGGA AGGAGAGGAG
    CCCACTGCAA CCGGCCCAGG CACCGCCATG GCCCACTCTA CCCCTCACCG CTACATCTCA
    GAGCCCTACC TGCCACTGCA GGAGGACGTC AACAGTCCAG ACTCCAGTGG TGTGGAGGAG
    ACTCTCACTT TTGAGGCTGC AATAGCAACC AGCCTGGGCC GCTCTAATAC CATTAGCTCG
    GCGCCACAGC TACGTCACTC CTGGCAGGTG CCTAATGGAC ATTTCCGCAA ACGACTAGCA
    CAAGCCACAG CTGGGACGTC CGGCTGTGAG CTTCTCAGTG ACACCGATGA GGATGACCGC
    TGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334062.1
    CDS388..7593
    Translation

    Target ORF information:

    RefSeq Version XM_021478387.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478387.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTTGGAAT TGGGCACTTG TTCGGTCAGC GCTCAGACTC TCTCTGACCG TAATGCCCTG 
    GCGGTCCCGT CATTTGGTCA GGGCATATGT GGCGGGAGCC CACCTTCACG ATGTTTCCTT
    CTCCAGCCGC AGGACCCGGA GAGCTGGCTC AAGCACCGGG GAACCCTGGA GGATGGCTCC
    CGCTCCTGTT CACTCCTGGA GCCTCAGAGC CTGGCTGACA GCCTTCTGTC CCATGCATCC
    AGTTTTGATA TGCTCTACGT CCCACATGGG CGTCCAATGG GCCTTGCACC GTCTTCTCTG
    GATCTGGGCG GCTCGTGTGA GGGGGCGGCC GCGTTGGGTC TGGACCCCGA CATGCCCGTC
    AGCCCGAATA TCATTAAACG GCGCAGGGGG GGACTCATTG AACAACGGGA CATCATTAAA
    GCACACCAGG CACACAAGAT CCAGAGCACC CCACAGGCCC GACGCAAGGA ATGGGAAATG
    GCTCGCTTCG GAGACGATGT TCCCGGTCTG CTGGGAGCCG GGGATGGGGG TTCGGAGCGC
    AGTCGGAACC GGGATGCTCC GGCGGTATCC ACAGGTGGAG TTTCACCAGC CTTCAAACAG
    ACCAAAGCGC AACGAGCCCG AACCATGGCT CTGTACAACC CCATTCCTGT CCGACAGAAC
    TGCTTCACCG TCAACAGATC TCTCTTCATC TTTGGAGAGG ACAACATCGT TAGGAAGTAT
    GCCAAGAAGA TCATAGAGTG GCCTCCGTTT GAATACATGA TCCTGGCCAC CATTATTGCT
    AACTGCATCG TCCTAGCCTT GGAGCAGCAC CTGCCTGGAG AAGACAAGAC ACCCATGGCT
    AAGAGACTTG AAAAAACAGA GCCCTACTTC ATAGGAATGT TTTGCTTTGA AGCTGGAATA
    AAAATCATTG CTTTGGGATT TGTATTCCAC AAGGGCTCGT ACCTGAGGAA TGGTTGGAAC
    GTCATGGACT TTATTGTCGT ATCGAGTGGG ATTCTGGCCA CAGCAGGAGC CCACATGAAT
    ATTTCATTAG ACCTGCGCAC ACTGAGGGCT GTGAGGGTAT TACGGCCCCT CAAACTGGTC
    TCTGGCATTC CTAGTCTGCA AATAGTTCTT AAATCCATAA TGAAGGCAAT GGTGCCATTG
    CTTCAGATCG GACTGCTACT GTTTTTTGCT ATTCTAATGT TTGCCATTAT TGGATTAGAG
    TTCTACAGTG GGAAACTCCA CAAGCAATGT GAACCTGTAG GTAAAGATTC TGAGAGAGCA
    GACTCGCCTG AGAATGAATT TCCATGTGGA AGCAAACTGT GTCCTGACCA TTATAACTGC
    TCTGGCTACT GGACGGGACC CAATGATGGC ATCACTCAGT TTGATAACAT CTTATTTTCT
    TTACTCACCG TCTTCCAGTG CATCACCATG GAAGGCTGGA CGACCATCCT CTATTACACC
    AACGATGCCT TAGGCTCTAC TTGGAATTGG CTCTACTTCA TTCCTCTAAT CATAATTGGC
    TCCTTCTTTG TGTTGAATCT GGTATTAGGA GTCCTGTCAG GGGAGTTTGC TAAAGAGCGA
    GAGCGGGTAG AGAACCGGAG AGCCTTCATG AAGCTTAGGA GACAGCAGCA GATTGAGAGG
    GAGCTGAACG GATATCGGGC CTGGATCGAC CGTGCAGAGG AGGTTATGCT TGCAGAGGAG
    AACAAGAATT CAGGACCTTC TGCTTTGGAT GTGCTAAAAA GAGCTACCAC CATAAAGAGA
    AGAGGAGTGG ATGATGCTCG AAGAGCTCCA GAAGAGCAGT ATGCTGACAT CTCATCTCTA
    GGGATGCCAC TGAACAGAGC CAGCATACGC AGTGCTAAAA GAGGACCAAC ATCCTATTTC
    CGACGGAAAG AGCGCCTGCT ACGGATCTCC GTCCGCCGTA TGGTGAAGAC TCAGACGTTC
    TACTGGACTG TTCTGGGTCT GGTAGCCCTC AACACTCTCT GTGTAGCCAT CGTACATCAC
    AAACAGCCAC AGTGGTTATC TAGTCTCCTC TACTATGCAG AGTTTTTGTT TCTGGGTTTG
    TTTCTTACCG AGATGTTTCT GAAGATGTAT GGACTCGGAC CCAGACTCTA TTTTCACTCC
    TCCTTCAACT GCTTCGATTG TGGGGTTATA GTAGGCAGCA TCTTTGAGGT GTTCTGGGGC
    TTCTTCAGAC CTGGCACATC CTTTGGGATC AGCGTTCTTC GTGCCTTGCG GTTGTTGAGA
    ATTTTCAAGA TAACCAAATA CTGGGCGTCC CTGAGGAACC TGGTCGTGTC TCTGATGAAC
    TCAATGAAGT CTATTATCAG TTTGCTCTTC CTGCTTTTTC TCTTTATTGT GGTCTTTGCA
    CTTCTGGGCA TGCAGCTCTT TGGTGGAAGG TTCATTTTTG ATGAGTACAC TCCCACCAAT
    TTTGACACTT TCCCAGCAGC CATCATGACA GTGTTTCAAA TTCTGACTGG TGAGGACTGG
    AATGAAGTCA TGTACAATGG CATCCGGTCT CAAGGTGGCG TGAAATCTGG GATGTGGTCG
    TCTGTCTACT TCATTGTTCT CACTCTGTTT GGAAATTATA CGCTGCTTAA CGTCTTCTTG
    GCTATCGCTG TCGACAATTT GGCCAATGCC CAGGAGCTCA CAAAGGACGA AGAGGAGGAG
    GAAGAGGCGT TTAATCAGAA ACATGCCCTG CAGAAAGCCA AGGAGGTTGG GCCCATGTCC
    TCTTTCATGT CCTCAGAGAG AGAGAGGAGG AGGAGGTTGA CCATGTCAGT GTGGGAGCAG
    AGGACCAGCC AGCTGCGGCG GCACAGGCAG ATGTCCAGCC GCGAGATCCT TTTCAGCAGC
    CCCAGTGAAG AACAGGACGG ACCACCAGGC TGCCCTCTCC ACCGGAAACC TGGACTATCT
    CCATCTGGCA GCAAGCGCAG AGCCACTGAG ACCACTTCAA CCACAATCAT GCCAGAGGGT
    ACCTTAGAGC CACCCATGCC TATGGACATG CCGGTGGATG AGCAAGCTCT GCCCATTTCT
    ATTCCTGAAC CCCCTTTAGC CATTCCAATT CCAGAGCCAC CAATGCCCGT GGACGCATGC
    CTCCCTGAAC CCCCAGTTAC ACTGAATCTG CCCTTAACAG AGCCATCTGA ATCAAAGACT
    TCACCCGAGG GTGCTCTGGA CATCAACAAC CATTGCCCTA GACTAAATGG GAACAGGCGG
    CAGCGGGCTG TCCGTAAGCA CAGACCTCCT GCTATGGGGG ACATGCTGAC CCCTGATATG
    GGGCCCAGGC CCCGACGGCC ACGCCACCGC GAGCTTCACA CAGACCCCAG GGGAAAAGAG
    ACTCTGCAAG GTGATGGTGT TGACGGTAAA GAACAAAAGC CAGGAAGTGA GGGTGATGAA
    AAGAGTGATG GAGAAATCAG TCCAGATGGC AGAGGGCTTG AACAAAGGCA GAACAAACCA
    GACAACACAG AATCATCCAC AGAGTCCAAA CCAGCAAATT CCCCCCGCAA AATCCCACCT
    CTTGACTCTG AACCTGCTAC TCTACAGCTC ACTGACAACA CACTGCAGCA GTCTTCACAG
    GGAACTACAT GTGCCGGTGA GACCGATAAC AATAACCACG GGTCCTTGAA TCAGGATAAG
    TCCTCCCTCA ACACCAGCAT CGTGATAGAG GTCACCGACA CTCAGCCATC CATGGAGCTG
    ACCTCCATTA CGGGTAATAG TAAAGATGTG GAGGCCTCAA AGCCAGAGAA AGAAGAAGAA
    ACCCCAGATG CACCCAAAGG GCCTCCACCT GTCAGCATGT TCATCTTCAA ACCCAACAAC
    CCGGTCCGCA GGGCCTGCCA CTACGTTGTC AACCTTCGTT ATTTTGAGAT GTCAATCCTT
    CTTGTGATCG CCGCCAGCAG TATCGCATTG GCTGCCGAAG ACCCCGTCAA CACTAATTCG
    GACAGGAACA AAGTTCTGCG CTATTTTGAC TATGTATTCA CTGGGGTCTT CACGTTTGAA
    ATGATAATCA AGATGATTGA TCAGGGGTTG GTCTTACACT ATGGCTCCTA TTTTCGTGAC
    CCGTGGAACA TTCTGGATTT CATAGTTGTG GTGGGAGCTC TGGTGGCTTT TGCTCTCACG
    AATGTGATGG GGAGTAACAC AGGAAGAGAC ATTAAAACCA TTAAATCGCT GCGTGTACTC
    CGAGTTCTGA GGCCTCTCAA GACGATTAAA AGACTTCCTA AACTCAAGGC TGTGTTTGAC
    TGTGTGGTAA CATCACTGAA GAATGTTTTC AACATCCTCA TCGTCTACAA GCTGTTTATG
    TTCATATTTG CCGTCATTGC CGTGCAGCTT TTCAAAGGGA AATTTTACTA CTGTACCGAC
    AGTTCCAAGG ACACTGAAAA CGAGTGCAAA GGCTACTACA TTGACTATGA TAAAGACAAG
    AAAAAGGAGA AACGAGAGTG GAAGAGACGC GATTTCCATT ATGATAACGT TGTCTGGGCT
    CTGTTGACTC TCTTCACCGT ATCCACTGGC GAAGGCTGGC CGCAAGTTCT GCAACACTCT
    GTGGATGTGA CAGAGGAGGA CCGGGGTCCC AGTCAGGGGA ACAGGATGGA GATGTCTATC
    TTCTATGTCA TTTATTTTGT GGTGTTCCCC TTCTTCTTCG TCAACATATT TGTGGCCCTC
    ATCATCATCA CTTTCCAAGA ACAGGGTGAC AAAATGATGG AGGAGTGTAG TCTGGAGAAA
    AACGAGAGGG CTTGTATCGA CTTTGCCATC AGTGCCAAAC CTTTGACTCG CTACATGCCC
    CAGAACAGAC AGACCTTACA GTACAGACTG TGGCAATTTG TGGTGTCGCC CTCATTTGAG
    TACACAGTAC TGACAATGAT CGCCCTTAAC ACCATCGTTC TCATGATGAA GCATCATGAT
    CCTCGTCTTG AAGGCTATGA CGATGTGCTC AAATATCTCA ACACAATTTT CACCGTTATT
    TTCTCTGTTG AGTGTGTTCT CAAGATCCTG GCTTTTGGTA TAATGAACTA TTTCAGAGAC
    ACATGGAATA TATTTGACTT CATCACGGTT CTAGGAAGCA TCACAGAAAT TCTGGTGGAC
    AAATTTCAGA CTGGAAAAAC TTTTAATATG GGCTTCCTGA AGCTATTCCG GGCAGCTCGG
    CTGATTAAAC TCCTGCGTCA GGGCTACACC ATCCGTATTC TGCTCTGGAC TTTTGTTCAG
    TCCTTCAAGG CTCTACCATA TGTCTGTCTG CTCATCGCCA TGCTGTTTTT CATCTATGCC
    ATCATTGGCA TGCAGGTATT TGGCAACATC AAGATAAATG AGAAAACCCA AATCAATGAA
    CACAATAACT TCAGGACTTT CTTTGGTGCA CTCATGCTTT TGTTCAGGAG TGCAACAGGT
    GAATCATGGC AGGAAATCAT GCTATCGTGT TTAGGAGAGA AGGAGTGTGA AATTGACACT
    TCTGTCAACT CAACAACAAA GACAATAAAA AACGACTGCG GTACCGACTT TGCCTACTTC
    TACTTTGTCT CCTTCATCTT CTTCAGTTCT TTTCTGATGC TGAATCTGTT TGTGGCTGTG
    ATCATGGATA ATTTTGAGTA CCTGACACGT GACTCATCCA TTCTGGGTCC TCACCACTTA
    GATGAGTTTG TACGGATCTG GGGAGAGTAT GACCGTGCTG CATGTGGCCG TATTCATTAC
    ACTGATATGT ACGAGATGCT GACCTGCATG TCGCCACCGC TTGGCCTGGG CAAGAAATGT
    CCCTCCAAAG TGGCGTATAA GCGGTTGGTT CTGATGAACA TGCCAGTGGA TGAAGACATG
    TCTGTCCACT TCACCTCCAC ACTTATGGCC CTCATACGCA CCGCACTGGA CATTAAAATT
    GCTCGAGGAG GGGAAGACAG GGGTCAGATG GATGTTGAAC TGCAGAAGGA AATCAGTGTC
    ATCTGGCCGC ATCTCTCACA GAAATCTCTT GATCTGCTTG TACCCATTCA CAAAGCTAGT
    GACATGACCA TTGGGAAGAT CTATGCAGCC ATGATGATCA TGGACTACTA CAAGCAGAAC
    AAAGCCAAGA AGCTCCGTCA GCAGCTCGAG GAACAGAAAC ACGCACCAAT GTTCCAACGC
    ATGGATGCAT CCTCGCTGCC GCAGGACATC CTCTGCAGTG CCAAGGCTTT GCCATTCCTG
    ACTCACAGTG CAGGATCTGC CCTCACTAGA GGTGGGTTTG TGGCACTGAG TCCTATCTCT
    CCTCAAGATC TCTTCATGCA GCCCATGACC CAAGACAGCA TGGAGGAGAG GGAGGAATAC
    CAACACCCGT ATCACCTGCA AAGCATGGTG CCTGAGCACA AAGAATTTAG CAGTGTGATG
    TGCCCCACTA GCATGCAGGC TGATCCACTG CAAGTGCCAG GAAGAGGCGT CTCTATCCGA
    GCCTCTTCCA TGCCTCGCCT GGCGGTAGAG CCACAGGCCT CTTTAGAGAA CAAACTGATG
    AAGCGCTCAT TCTCCACCAT CGGGGATCAG TGTGTAAATG GTGACTGGCA GGAAGAGTTC
    TCTCTGGAGA GGGTGAGTCC TGACCGGCTG GAGAGACCCA GGCAAAGAAG CTTCAGAGGT
    GTCAGGTCAA ACCTGCGGGA GCCAGTGGAC AAAGAGAGAG GACGGTCTAA AGAACGAAGA
    CACCTGCTGT CTCCAGATGT GTCTCGCTGC AACTCAGAGG AAAGGAGTCA GTCTCAGGAG
    AGACGTCATT CTCCATCGCC CAGTGAGGGG AGGAAGCACA CAGCTCTCAG ACAGGGAAAT
    AATACAGGCA GCCCTATTGC CTCCACGTCT GATTCAGGGA CGCCTCGAGG ACGCCGCCAA
    CTCCCACAGA CACCCTCTCG CCCTCGGCCT CATATCTCCT ACTCTCCTTT AGTGTGCCGT
    GCCCATCCAG CACCAGGGGA TGATCGGCCA CGAGCTCCAC CCGCAGAGGA AGGAGAGGAG
    CCCACTGCAA CCGGCCCAGG CACCGCCATG GCCCACTCTA CCCCTCACCG CTACATCTCA
    GAGCCCTACC TGCCACTGCA GGAGGACGTC AACAGTCCAG ACTCCAGTGG TGTGGAGGAG
    ACTCTCACTT TTGAGGCTGC AATAGCAACC AGCCTGGGCC GCTCTAATAC CATTAGCTCG
    GCGCCACAGC TACGTCACTC CTGGCAGGTG CCTAATGGAC ATTTCCGCAA ACGACTAGCA
    CAAGCCACAG CTGGGACGTC CGGCTGTGAG CTTCTCAGTG ACACCGATGA GGATGACCGC
    TGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44281
    Clone ID Related Accession (Same CDS sequence) XM_021478388.1
    Accession Version XM_021478388.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTTGGAAT TGGGCACTTG TTCGGTCAGC GCTCAGACTC TCTCTGACCG TAATGCCCTG 
    GCGGTCCCGT CATTTGGTCA GGGCATATGT GGCGGGAGCC CACCTTCACG ATGTTTCCTT
    CTCCAGCCGC AGGACCCGGA GAGCTGGCTC AAGCACCGGG GAACCCTGGA GGATGGCTCC
    CGCTCCTGTT CACTCCTGGA GCCTCAGAGC CTGGCTGACA GCCTTCTGTC CCATGCATCC
    AGTTTTGATA TGCTCTACGT CCCACATGGG CGTCCAATGG GCCTTGCACC GTCTTCTCTG
    GATCTGGGCG GCTCGTGTGA GGGGGCGGCC GCGTTGGGTC TGGACCCCGA CATGCCCGTC
    AGCCCGAATA TCATTAAACG GCGCAGGGGG GGACTCATTG AACAACGGGA CATCATTAAA
    GCACACCAGG CACACAAGAT CCAGAGCACC CCACAGGCCC GACGCAAGGA ATGGGAAATG
    GCTCGCTTCG GAGACGATGT TCCCGGTCTG CTGGGAGCCG GGGATGGGGG TTCGGAGCGC
    AGTCGGAACC GGGATGCTCC GGCGGTATCC ACAGGTGGAG TTTCACCAGC CTTCAAACAG
    ACCAAAGCGC AACGAGCCCG AACCATGGCT CTGTACAACC CCATTCCTGT CCGACAGAAC
    TGCTTCACCG TCAACAGATC TCTCTTCATC TTTGGAGAGG ACAACATCGT TAGGAAGTAT
    GCCAAGAAGA TCATAGAGTG GCCTCCGTTT GAATACATGA TCCTGGCCAC CATTATTGCT
    AACTGCATCG TCCTAGCCTT GGAGCAGCAC CTGCCTGGAG AAGACAAGAC ACCCATGGCT
    AAGAGACTTG AAAAAACAGA GCCCTACTTC ATAGGAATGT TTTGCTTTGA AGCTGGAATA
    AAAATCATTG CTTTGGGATT TGTATTCCAC AAGGGCTCGT ACCTGAGGAA TGGTTGGAAC
    GTCATGGACT TTATTGTCGT ATCGAGTGGG ATTCTGGCCA CAGCAGGAGC CCACATGAAT
    ATTTCATTAG ACCTGCGCAC ACTGAGGGCT GTGAGGGTAT TACGGCCCCT CAAACTGGTC
    TCTGGCATTC CTAGTCTGCA AATAGTTCTT AAATCCATAA TGAAGGCAAT GGTGCCATTG
    CTTCAGATCG GACTGCTACT GTTTTTTGCT ATTCTAATGT TTGCCATTAT TGGATTAGAG
    TTCTACAGTG GGAAACTCCA CAAGCAATGT GAACCTGTAG GTAAAGATTC TGAGAGAGCA
    GACTCGCCTG AGAATGAATT TCCATGTGGA AGCAAACTGT GTCCTGACCA TTATAACTGC
    TCTGGCTACT GGACGGGACC CAATGATGGC ATCACTCAGT TTGATAACAT CTTATTTTCT
    TTACTCACCG TCTTCCAGTG CATCACCATG GAAGGCTGGA CGACCATCCT CTATTACACC
    AACGATGCCT TAGGCTCTAC TTGGAATTGG CTCTACTTCA TTCCTCTAAT CATAATTGGC
    TCCTTCTTTG TGTTGAATCT GGTATTAGGA GTCCTGTCAG GGGAGTTTGC TAAAGAGCGA
    GAGCGGGTAG AGAACCGGAG AGCCTTCATG AAGCTTAGGA GACAGCAGCA GATTGAGAGG
    GAGCTGAACG GATATCGGGC CTGGATCGAC CGTGCAGAGG AGGTTATGCT TGCAGAGGAG
    AACAAGAATT CAGGACCTTC TGCTTTGGAT GTGCTAAAAA GAGCTACCAC CATAAAGAGA
    AGAGGAGTGG ATGATGCTCG AAGAGCTCCA GAAGAGCAGT ATGCTGACAT CTCATCTCTA
    GGGATGCCAC TGAACAGAGC CAGCATACGC AGTGCTAAAA GAGGACCAAC ATCCTATTTC
    CGACGGAAAG AGCGCCTGCT ACGGATCTCC GTCCGCCGTA TGGTGAAGAC TCAGACGTTC
    TACTGGACTG TTCTGGGTCT GGTAGCCCTC AACACTCTCT GTGTAGCCAT CGTACATCAC
    AAACAGCCAC AGTGGTTATC TAGTCTCCTC TACTATGCAG AGTTTTTGTT TCTGGGTTTG
    TTTCTTACCG AGATGTTTCT GAAGATGTAT GGACTCGGAC CCAGACTCTA TTTTCACTCC
    TCCTTCAACT GCTTCGATTG TGGGGTTATA GTAGGCAGCA TCTTTGAGGT GTTCTGGGGC
    TTCTTCAGAC CTGGCACATC CTTTGGGATC AGCGTTCTTC GTGCCTTGCG GTTGTTGAGA
    ATTTTCAAGA TAACCAAATA CTGGGCGTCC CTGAGGAACC TGGTCGTGTC TCTGATGAAC
    TCAATGAAGT CTATTATCAG TTTGCTCTTC CTGCTTTTTC TCTTTATTGT GGTCTTTGCA
    CTTCTGGGCA TGCAGCTCTT TGGTGGAAGG TTCATTTTTG ATGAGTACAC TCCCACCAAT
    TTTGACACTT TCCCAGCAGC CATCATGACA GTGTTTCAAA TTCTGACTGG TGAGGACTGG
    AATGAAGTCA TGTACAATGG CATCCGGTCT CAAGGTGGCG TGAAATCTGG GATGTGGTCG
    TCTGTCTACT TCATTGTTCT CACTCTGTTT GGAAATTATA CGCTGCTTAA CGTCTTCTTG
    GCTATCGCTG TCGACAATTT GGCCAATGCC CAGGAGCTCA CAAAGGACGA AGAGGAGGAG
    GAAGAGGCGT TTAATCAGAA ACATGCCCTG CAGAAAGCCA AGGAGGTTGG GCCCATGTCC
    TCTTTCATGT CCTCAGAGAG GAGGAGGAGG CCCTACCTGT ACAGGAAGCG TGCAATTCAT
    CGGAGCCGTG CAGCCTACTC CTTCGAGGAT GCAGAGAGTC TGGCTGACGC TCGTCCATTC
    AATGCCTCTA ACTCTTTCAT GTCCAGGAGA GAGAGGAGGA GGAGGTTGAC CATGTCAGTG
    TGGGAGCAGA GGACCAGCCA GCTGCGGCGG CACAGGCAGA TGTCCAGCCG CGAGATCCTT
    TTCAGCAGCC CCAGTGAAGA ACAGGACGGA CCACCAGGCT GCCCTCTCCA CCGGAAACCT
    GGACTATCTC CATCTGGCAG CAAGCGCAGA GCCACTGAGA CCACTTCAAC CACAATCATG
    CCAGAGGGTA CCTTAGAGCC ACCCATGCCT ATGGACATGC CGGTGGATGA GCAAGCTCTG
    CCCATTTCTA TTCCTGAACC CCCTTTAGCC ATTCCAATTC CAGAGCCACC AATGCCCGTG
    GACGCATGCC TCCCTGAACC CCCAGTTACA CTGAATCTGC CCTTAACAGA GCCATCTGAA
    TCAAAGACTT CACCCGAGGG TGCTCTGGAC ATCAACAACC ATTGCCCTAG ACTAAATGGG
    AACAGGCGGC AGCGGGCTGT CCGTAAGCAC AGACCTCCTG CTATGGGGGA CATGCTGACC
    CCTGATATGG GGCCCAGGCC CCGACGGCCA CGCCACCGCG AGCTTCACAC AGACCCCAGG
    GGAAAAGAGA CTCTGCAAGG TGATGGTGTT GACGGTAAAG AACAAAAGCC AGGAAGTGAG
    GGTGATGAAA AGAGTGATGG AGAAATCAGT CCAGATGGCA GAGGGCTTGA ACAAAGGCAG
    AACAAACCAG ACAACACAGA ATCATCCACA GAGTCCAAAC CAGCAAATTC CCCCCGCAAA
    ATCCCACCTC TTGACTCTGA ACCTGCTACT CTACAGCTCA CTGACAACAC ACTGCAGCAG
    TCTTCACAGG GAACTACATG TGCCGGTGAG ACCGATAACA ATAACCACGG GTCCTTGAAT
    CAGGATAAGT CCTCCCTCAA CACCAGCATC GTGATAGAGG TCACCGACAC TCAGCCATCC
    ATGGAGCTGA CCTCCATTAC GGGTAATAGT AAAGATGTGG AGGCCTCAAA GCCAGAGAAA
    GAAGAAGAAA CCCCAGATGC ACCCAAAGGG CCTCCACCTG TCAGCATGTT CATCTTCAAA
    CCCAACAACC CGGTCCGCAG GGCCTGCCAC TACGTTGTCA ACCTTCGTTA TTTTGAGATG
    TCAATCCTTC TTGTGATCGC CGCCAGCAGT ATCGCATTGG CTGCCGAAGA CCCCGTCAAC
    ACTAATTCGG ACAGGAACAA AGTTCTGCGC TATTTTGACT ATGTATTCAC TGGGGTCTTC
    ACGTTTGAAA TGATAATCAA GATGATTGAT CAGGGGTTGG TCTTACACTA TGGCTCCTAT
    TTTCGTGACC CGTGGAACAT TCTGGATTTC ATAGTTGTGG TGGGAGCTCT GGTGGCTTTT
    GCTCTCACGA ATGTGATGGG GAGTAACACA GGAAGAGACA TTAAAACCAT TAAATCGCTG
    CGTGTACTCC GAGTTCTGAG GCCTCTCAAG ACGATTAAAA GACTTCCTAA ACTCAAGGCT
    GTGTTTGACT GTGTGGTAAC ATCACTGAAG AATGTTTTCA ACATCCTCAT CGTCTACAAG
    CTGTTTATGT TCATATTTGC CGTCATTGCC GTGCAGCTTT TCAAAGGGAA ATTTTACTAC
    TGTACCGACA GTTCCAAGGA CACTGAAAAC GAGTGCAAAG GCTACTACAT TGACTATGAT
    AAAGACAAGA AAAAGGAGAA ACGAGAGTGG AAGAGACGCG ATTTCCATTA TGATAACGTT
    GTCTGGGCTC TGTTGACTCT CTTCACCGTA TCCACTGGCG AAGGCTGGCC GCAAGTTCTG
    CAACACTCTG TGGATGTGAC AGAGGAGGAC CGGGGTCCCA GTCAGGGGAA CAGGATGGAG
    ATGTCTATCT TCTATGTCAT TTATTTTGTG GTGTTCCCCT TCTTCTTCGT CAACATATTT
    GTGGCCCTCA TCATCATCAC TTTCCAAGAA CAGGGTGACA AAATGATGGA GGAGTGTAGT
    CTGGAGAAAA ACGAGAGGGC TTGTATCGAC TTTGCCATCA GTGCCAAACC TTTGACTCGC
    TACATGCCCC AGAACAGACA GACCTTACAG TACAGACTGT GGCAATTTGT GGTGTCGCCC
    TCATTTGAGT ACACAGTACT GACAATGATC GCCCTTAACA CCATCGTTCT CATGATGAAG
    CATCATGATC CTCGTCTTGA AGGCTATGAC GATGTGCTCA AATATCTCAA CACAATTTTC
    ACCGTTATTT TCTCTGTTGA GTGTGTTCTC AAGATCCTGG CTTTTGGTAT AATGAACTAT
    TTCAGAGACA CATGGAATAT ATTTGACTTC ATCACGGTTC TAGGAAGCAT CACAGAAATT
    CTGGTGGACA AATTTCAGAC TGGAAAAACT TTTAATATGG GCTTCCTGAA GCTATTCCGG
    GCAGCTCGGC TGATTAAACT CCTGCGTCAG GGCTACACCA TCCGTATTCT GCTCTGGACT
    TTTGTTCAGT CCTTCAAGGC TCTACCATAT GTCTGTCTGC TCATCGCCAT GCTGTTTTTC
    ATCTATGCCA TCATTGGCAT GCAGGTATTT GGCAACATCA AGATAAATGA GAAAACCCAA
    ATCAATGAAC ACAATAACTT CAGGACTTTC TTTGGTGCAC TCATGCTTTT GTTCAGGAGT
    GCAACAGGTG AATCATGGCA GGAAATCATG CTATCGTGTT TAGGAGAGAA GGAGTGTGAA
    ATTGACACTT CTGTCAACTC AACAACAAAG ACAATAAAAA ACGACTGCGG TACCGACTTT
    GCCTACTTCT ACTTTGTCTC CTTCATCTTC TTCAGTTCTT TTCTGATGCT GAATCTGTTT
    GTGGCTGTGA TCATGGATAA TTTTGAGTAC CTGACACGTG ACTCATCCAT TCTGGGTCCT
    CACCACTTAG ATGAGTTTGT ACGGATCTGG GGAGAGTATG ACCGTGCTGC ATGTGGCCGT
    ATTCATTACA CTGATATGTA CGAGATGCTG ACCTGCATGT CGCCACCGCT TGGCCTGGGC
    AAGAAATGTC CCTCCAAAGT GGCGTATAAG CGGTTGGTTC TGATGAACAT GCCAGTGGAT
    GAAGACATGT CTGTCCACTT CACCTCCACA CTTATGGCCC TCATACGCAC CGCACTGGAC
    ATTAAAATTG CTCGAGGAGG GGAAGACAGG GGTCAGATGG ATGTTGAACT GCAGAAGGAA
    ATCAGTGTCA TCTGGCCGCA TCTCTCACAG AAATCTCTTG ATCTGCTTGT ACCCATTCAC
    AAAGCTAGTG ACATGACCAT TGGGAAGATC TATGCAGCCA TGATGATCAT GGACTACTAC
    AAGCAGAACA AAGCCAAGAA GCTCCGTCAG CAGCTCGAGG AACAGAAACA CGCACCAATG
    TTCCAACGCA TGGATGCATC CTCGCTGCCG CAGGACATCC TCTGCAGTGC CAAGGCTTTG
    CCATTCCTGA CTCACAGTGC AGGATCTGCC CTCACTAGAG GTGGGTTTGT GGCACTGAGT
    CCTATCTCTC CTCAAGATCT CTTCATGCAG CCCATGACCC AAGACAGCAT GGAGGAGAGG
    GAGGAATACC AACACCCGTA TCACCTGCAA AGCATGGCCT CTTTAGAGAA CAAACTGATG
    AAGCGCTCAT TCTCCACCAT CGGGGATCAG TGTGTAAATG GTGACTGGCA GGAAGAGTTC
    TCTCTGGAGA GGGTGAGTCC TGACCGGCTG GAGAGACCCA GGCAAAGAAG CTTCAGAGGT
    GTCAGGTCAA ACCTGCGGGA GCCAGTGGAC AAAGAGAGAG GACGGTCTAA AGAACGAAGA
    CACCTGCTGT CTCCAGATGT GTCTCGCTGC AACTCAGAGG AAAGGAGTCA GTCTCAGGAG
    AGACGTCATT CTCCATCGCC CAGTGAGGGG AGGAAGCACA CAGCTCTCAG ACAGGGAAAT
    AATACAGGCA GCCCTATTGC CTCCACGTCT GATTCAGGGA CGCCTCGAGG ACGCCGCCAA
    CTCCCACAGA CACCCTCTCG CCCTCGGCCT CATATCTCCT ACTCTCCTTT AGTGTGCCGT
    GCCCATCCAG CACCAGGGGA TGATCGGCCA CGAGCTCCAC CCGCAGAGGA AGGAGAGGAG
    CCCACTGCAA CCGGCCCAGG CACCGCCATG GCCCACTCTA CCCCTCACCG CTACATCTCA
    GAGCCCTACC TGCCACTGCA GGAGGACGTC AACAGTCCAG ACTCCAGTGG TGTGGAGGAG
    ACTCTCACTT TTGAGGCTGC AATAGCAACC AGCCTGGGCC GCTCTAATAC CATTAGCTCG
    GCGCCACAGC TACGTCACTC CTGGCAGGTG CCTAATGGAC ATTTCCGCAA ACGACTAGCA
    CAAGCCACAG CTGGGACGTC CGGCTGTGAG CTTCTCAGTG ACACCGATGA GGATGACCGC
    TGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334063.1
    CDS388..7593
    Translation

    Target ORF information:

    RefSeq Version XM_021478388.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478388.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGTTGGAAT TGGGCACTTG TTCGGTCAGC GCTCAGACTC TCTCTGACCG TAATGCCCTG 
    GCGGTCCCGT CATTTGGTCA GGGCATATGT GGCGGGAGCC CACCTTCACG ATGTTTCCTT
    CTCCAGCCGC AGGACCCGGA GAGCTGGCTC AAGCACCGGG GAACCCTGGA GGATGGCTCC
    CGCTCCTGTT CACTCCTGGA GCCTCAGAGC CTGGCTGACA GCCTTCTGTC CCATGCATCC
    AGTTTTGATA TGCTCTACGT CCCACATGGG CGTCCAATGG GCCTTGCACC GTCTTCTCTG
    GATCTGGGCG GCTCGTGTGA GGGGGCGGCC GCGTTGGGTC TGGACCCCGA CATGCCCGTC
    AGCCCGAATA TCATTAAACG GCGCAGGGGG GGACTCATTG AACAACGGGA CATCATTAAA
    GCACACCAGG CACACAAGAT CCAGAGCACC CCACAGGCCC GACGCAAGGA ATGGGAAATG
    GCTCGCTTCG GAGACGATGT TCCCGGTCTG CTGGGAGCCG GGGATGGGGG TTCGGAGCGC
    AGTCGGAACC GGGATGCTCC GGCGGTATCC ACAGGTGGAG TTTCACCAGC CTTCAAACAG
    ACCAAAGCGC AACGAGCCCG AACCATGGCT CTGTACAACC CCATTCCTGT CCGACAGAAC
    TGCTTCACCG TCAACAGATC TCTCTTCATC TTTGGAGAGG ACAACATCGT TAGGAAGTAT
    GCCAAGAAGA TCATAGAGTG GCCTCCGTTT GAATACATGA TCCTGGCCAC CATTATTGCT
    AACTGCATCG TCCTAGCCTT GGAGCAGCAC CTGCCTGGAG AAGACAAGAC ACCCATGGCT
    AAGAGACTTG AAAAAACAGA GCCCTACTTC ATAGGAATGT TTTGCTTTGA AGCTGGAATA
    AAAATCATTG CTTTGGGATT TGTATTCCAC AAGGGCTCGT ACCTGAGGAA TGGTTGGAAC
    GTCATGGACT TTATTGTCGT ATCGAGTGGG ATTCTGGCCA CAGCAGGAGC CCACATGAAT
    ATTTCATTAG ACCTGCGCAC ACTGAGGGCT GTGAGGGTAT TACGGCCCCT CAAACTGGTC
    TCTGGCATTC CTAGTCTGCA AATAGTTCTT AAATCCATAA TGAAGGCAAT GGTGCCATTG
    CTTCAGATCG GACTGCTACT GTTTTTTGCT ATTCTAATGT TTGCCATTAT TGGATTAGAG
    TTCTACAGTG GGAAACTCCA CAAGCAATGT GAACCTGTAG GTAAAGATTC TGAGAGAGCA
    GACTCGCCTG AGAATGAATT TCCATGTGGA AGCAAACTGT GTCCTGACCA TTATAACTGC
    TCTGGCTACT GGACGGGACC CAATGATGGC ATCACTCAGT TTGATAACAT CTTATTTTCT
    TTACTCACCG TCTTCCAGTG CATCACCATG GAAGGCTGGA CGACCATCCT CTATTACACC
    AACGATGCCT TAGGCTCTAC TTGGAATTGG CTCTACTTCA TTCCTCTAAT CATAATTGGC
    TCCTTCTTTG TGTTGAATCT GGTATTAGGA GTCCTGTCAG GGGAGTTTGC TAAAGAGCGA
    GAGCGGGTAG AGAACCGGAG AGCCTTCATG AAGCTTAGGA GACAGCAGCA GATTGAGAGG
    GAGCTGAACG GATATCGGGC CTGGATCGAC CGTGCAGAGG AGGTTATGCT TGCAGAGGAG
    AACAAGAATT CAGGACCTTC TGCTTTGGAT GTGCTAAAAA GAGCTACCAC CATAAAGAGA
    AGAGGAGTGG ATGATGCTCG AAGAGCTCCA GAAGAGCAGT ATGCTGACAT CTCATCTCTA
    GGGATGCCAC TGAACAGAGC CAGCATACGC AGTGCTAAAA GAGGACCAAC ATCCTATTTC
    CGACGGAAAG AGCGCCTGCT ACGGATCTCC GTCCGCCGTA TGGTGAAGAC TCAGACGTTC
    TACTGGACTG TTCTGGGTCT GGTAGCCCTC AACACTCTCT GTGTAGCCAT CGTACATCAC
    AAACAGCCAC AGTGGTTATC TAGTCTCCTC TACTATGCAG AGTTTTTGTT TCTGGGTTTG
    TTTCTTACCG AGATGTTTCT GAAGATGTAT GGACTCGGAC CCAGACTCTA TTTTCACTCC
    TCCTTCAACT GCTTCGATTG TGGGGTTATA GTAGGCAGCA TCTTTGAGGT GTTCTGGGGC
    TTCTTCAGAC CTGGCACATC CTTTGGGATC AGCGTTCTTC GTGCCTTGCG GTTGTTGAGA
    ATTTTCAAGA TAACCAAATA CTGGGCGTCC CTGAGGAACC TGGTCGTGTC TCTGATGAAC
    TCAATGAAGT CTATTATCAG TTTGCTCTTC CTGCTTTTTC TCTTTATTGT GGTCTTTGCA
    CTTCTGGGCA TGCAGCTCTT TGGTGGAAGG TTCATTTTTG ATGAGTACAC TCCCACCAAT
    TTTGACACTT TCCCAGCAGC CATCATGACA GTGTTTCAAA TTCTGACTGG TGAGGACTGG
    AATGAAGTCA TGTACAATGG CATCCGGTCT CAAGGTGGCG TGAAATCTGG GATGTGGTCG
    TCTGTCTACT TCATTGTTCT CACTCTGTTT GGAAATTATA CGCTGCTTAA CGTCTTCTTG
    GCTATCGCTG TCGACAATTT GGCCAATGCC CAGGAGCTCA CAAAGGACGA AGAGGAGGAG
    GAAGAGGCGT TTAATCAGAA ACATGCCCTG CAGAAAGCCA AGGAGGTTGG GCCCATGTCC
    TCTTTCATGT CCTCAGAGAG GAGGAGGAGG CCCTACCTGT ACAGGAAGCG TGCAATTCAT
    CGGAGCCGTG CAGCCTACTC CTTCGAGGAT GCAGAGAGTC TGGCTGACGC TCGTCCATTC
    AATGCCTCTA ACTCTTTCAT GTCCAGGAGA GAGAGGAGGA GGAGGTTGAC CATGTCAGTG
    TGGGAGCAGA GGACCAGCCA GCTGCGGCGG CACAGGCAGA TGTCCAGCCG CGAGATCCTT
    TTCAGCAGCC CCAGTGAAGA ACAGGACGGA CCACCAGGCT GCCCTCTCCA CCGGAAACCT
    GGACTATCTC CATCTGGCAG CAAGCGCAGA GCCACTGAGA CCACTTCAAC CACAATCATG
    CCAGAGGGTA CCTTAGAGCC ACCCATGCCT ATGGACATGC CGGTGGATGA GCAAGCTCTG
    CCCATTTCTA TTCCTGAACC CCCTTTAGCC ATTCCAATTC CAGAGCCACC AATGCCCGTG
    GACGCATGCC TCCCTGAACC CCCAGTTACA CTGAATCTGC CCTTAACAGA GCCATCTGAA
    TCAAAGACTT CACCCGAGGG TGCTCTGGAC ATCAACAACC ATTGCCCTAG ACTAAATGGG
    AACAGGCGGC AGCGGGCTGT CCGTAAGCAC AGACCTCCTG CTATGGGGGA CATGCTGACC
    CCTGATATGG GGCCCAGGCC CCGACGGCCA CGCCACCGCG AGCTTCACAC AGACCCCAGG
    GGAAAAGAGA CTCTGCAAGG TGATGGTGTT GACGGTAAAG AACAAAAGCC AGGAAGTGAG
    GGTGATGAAA AGAGTGATGG AGAAATCAGT CCAGATGGCA GAGGGCTTGA ACAAAGGCAG
    AACAAACCAG ACAACACAGA ATCATCCACA GAGTCCAAAC CAGCAAATTC CCCCCGCAAA
    ATCCCACCTC TTGACTCTGA ACCTGCTACT CTACAGCTCA CTGACAACAC ACTGCAGCAG
    TCTTCACAGG GAACTACATG TGCCGGTGAG ACCGATAACA ATAACCACGG GTCCTTGAAT
    CAGGATAAGT CCTCCCTCAA CACCAGCATC GTGATAGAGG TCACCGACAC TCAGCCATCC
    ATGGAGCTGA CCTCCATTAC GGGTAATAGT AAAGATGTGG AGGCCTCAAA GCCAGAGAAA
    GAAGAAGAAA CCCCAGATGC ACCCAAAGGG CCTCCACCTG TCAGCATGTT CATCTTCAAA
    CCCAACAACC CGGTCCGCAG GGCCTGCCAC TACGTTGTCA ACCTTCGTTA TTTTGAGATG
    TCAATCCTTC TTGTGATCGC CGCCAGCAGT ATCGCATTGG CTGCCGAAGA CCCCGTCAAC
    ACTAATTCGG ACAGGAACAA AGTTCTGCGC TATTTTGACT ATGTATTCAC TGGGGTCTTC
    ACGTTTGAAA TGATAATCAA GATGATTGAT CAGGGGTTGG TCTTACACTA TGGCTCCTAT
    TTTCGTGACC CGTGGAACAT TCTGGATTTC ATAGTTGTGG TGGGAGCTCT GGTGGCTTTT
    GCTCTCACGA ATGTGATGGG GAGTAACACA GGAAGAGACA TTAAAACCAT TAAATCGCTG
    CGTGTACTCC GAGTTCTGAG GCCTCTCAAG ACGATTAAAA GACTTCCTAA ACTCAAGGCT
    GTGTTTGACT GTGTGGTAAC ATCACTGAAG AATGTTTTCA ACATCCTCAT CGTCTACAAG
    CTGTTTATGT TCATATTTGC CGTCATTGCC GTGCAGCTTT TCAAAGGGAA ATTTTACTAC
    TGTACCGACA GTTCCAAGGA CACTGAAAAC GAGTGCAAAG GCTACTACAT TGACTATGAT
    AAAGACAAGA AAAAGGAGAA ACGAGAGTGG AAGAGACGCG ATTTCCATTA TGATAACGTT
    GTCTGGGCTC TGTTGACTCT CTTCACCGTA TCCACTGGCG AAGGCTGGCC GCAAGTTCTG
    CAACACTCTG TGGATGTGAC AGAGGAGGAC CGGGGTCCCA GTCAGGGGAA CAGGATGGAG
    ATGTCTATCT TCTATGTCAT TTATTTTGTG GTGTTCCCCT TCTTCTTCGT CAACATATTT
    GTGGCCCTCA TCATCATCAC TTTCCAAGAA CAGGGTGACA AAATGATGGA GGAGTGTAGT
    CTGGAGAAAA ACGAGAGGGC TTGTATCGAC TTTGCCATCA GTGCCAAACC TTTGACTCGC
    TACATGCCCC AGAACAGACA GACCTTACAG TACAGACTGT GGCAATTTGT GGTGTCGCCC
    TCATTTGAGT ACACAGTACT GACAATGATC GCCCTTAACA CCATCGTTCT CATGATGAAG
    CATCATGATC CTCGTCTTGA AGGCTATGAC GATGTGCTCA AATATCTCAA CACAATTTTC
    ACCGTTATTT TCTCTGTTGA GTGTGTTCTC AAGATCCTGG CTTTTGGTAT AATGAACTAT
    TTCAGAGACA CATGGAATAT ATTTGACTTC ATCACGGTTC TAGGAAGCAT CACAGAAATT
    CTGGTGGACA AATTTCAGAC TGGAAAAACT TTTAATATGG GCTTCCTGAA GCTATTCCGG
    GCAGCTCGGC TGATTAAACT CCTGCGTCAG GGCTACACCA TCCGTATTCT GCTCTGGACT
    TTTGTTCAGT CCTTCAAGGC TCTACCATAT GTCTGTCTGC TCATCGCCAT GCTGTTTTTC
    ATCTATGCCA TCATTGGCAT GCAGGTATTT GGCAACATCA AGATAAATGA GAAAACCCAA
    ATCAATGAAC ACAATAACTT CAGGACTTTC TTTGGTGCAC TCATGCTTTT GTTCAGGAGT
    GCAACAGGTG AATCATGGCA GGAAATCATG CTATCGTGTT TAGGAGAGAA GGAGTGTGAA
    ATTGACACTT CTGTCAACTC AACAACAAAG ACAATAAAAA ACGACTGCGG TACCGACTTT
    GCCTACTTCT ACTTTGTCTC CTTCATCTTC TTCAGTTCTT TTCTGATGCT GAATCTGTTT
    GTGGCTGTGA TCATGGATAA TTTTGAGTAC CTGACACGTG ACTCATCCAT TCTGGGTCCT
    CACCACTTAG ATGAGTTTGT ACGGATCTGG GGAGAGTATG ACCGTGCTGC ATGTGGCCGT
    ATTCATTACA CTGATATGTA CGAGATGCTG ACCTGCATGT CGCCACCGCT TGGCCTGGGC
    AAGAAATGTC CCTCCAAAGT GGCGTATAAG CGGTTGGTTC TGATGAACAT GCCAGTGGAT
    GAAGACATGT CTGTCCACTT CACCTCCACA CTTATGGCCC TCATACGCAC CGCACTGGAC
    ATTAAAATTG CTCGAGGAGG GGAAGACAGG GGTCAGATGG ATGTTGAACT GCAGAAGGAA
    ATCAGTGTCA TCTGGCCGCA TCTCTCACAG AAATCTCTTG ATCTGCTTGT ACCCATTCAC
    AAAGCTAGTG ACATGACCAT TGGGAAGATC TATGCAGCCA TGATGATCAT GGACTACTAC
    AAGCAGAACA AAGCCAAGAA GCTCCGTCAG CAGCTCGAGG AACAGAAACA CGCACCAATG
    TTCCAACGCA TGGATGCATC CTCGCTGCCG CAGGACATCC TCTGCAGTGC CAAGGCTTTG
    CCATTCCTGA CTCACAGTGC AGGATCTGCC CTCACTAGAG GTGGGTTTGT GGCACTGAGT
    CCTATCTCTC CTCAAGATCT CTTCATGCAG CCCATGACCC AAGACAGCAT GGAGGAGAGG
    GAGGAATACC AACACCCGTA TCACCTGCAA AGCATGGCCT CTTTAGAGAA CAAACTGATG
    AAGCGCTCAT TCTCCACCAT CGGGGATCAG TGTGTAAATG GTGACTGGCA GGAAGAGTTC
    TCTCTGGAGA GGGTGAGTCC TGACCGGCTG GAGAGACCCA GGCAAAGAAG CTTCAGAGGT
    GTCAGGTCAA ACCTGCGGGA GCCAGTGGAC AAAGAGAGAG GACGGTCTAA AGAACGAAGA
    CACCTGCTGT CTCCAGATGT GTCTCGCTGC AACTCAGAGG AAAGGAGTCA GTCTCAGGAG
    AGACGTCATT CTCCATCGCC CAGTGAGGGG AGGAAGCACA CAGCTCTCAG ACAGGGAAAT
    AATACAGGCA GCCCTATTGC CTCCACGTCT GATTCAGGGA CGCCTCGAGG ACGCCGCCAA
    CTCCCACAGA CACCCTCTCG CCCTCGGCCT CATATCTCCT ACTCTCCTTT AGTGTGCCGT
    GCCCATCCAG CACCAGGGGA TGATCGGCCA CGAGCTCCAC CCGCAGAGGA AGGAGAGGAG
    CCCACTGCAA CCGGCCCAGG CACCGCCATG GCCCACTCTA CCCCTCACCG CTACATCTCA
    GAGCCCTACC TGCCACTGCA GGAGGACGTC AACAGTCCAG ACTCCAGTGG TGTGGAGGAG
    ACTCTCACTT TTGAGGCTGC AATAGCAACC AGCCTGGGCC GCTCTAATAC CATTAGCTCG
    GCGCCACAGC TACGTCACTC CTGGCAGGTG CCTAATGGAC ATTTCCGCAA ACGACTAGCA
    CAAGCCACAG CTGGGACGTC CGGCTGTGAG CTTCTCAGTG ACACCGATGA GGATGACCGC
    TGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44282
    Clone ID Related Accession (Same CDS sequence) XM_017357424.2
    Accession Version XM_017357424.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6858bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357424.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCTCGCT TCGGAGACGA TGTTCCCGGT CTGCTGGGAG CCGGGGATGG GGGTTCGGAG 
    CGCAGTCGGA ACCGGGATGC TCCGGCGGTA TCCACAGGTG GAGTTTCACC AGCCTTCAAA
    CAGACCAAAG CGCAACGAGC CCGAACCATG GCTCTGTACA ACCCCATTCC TGTCCGACAG
    AACTGCTTCA CCGTCAACAG ATCTCTCTTC ATCTTTGGAG AGGACAACAT CGTTAGGAAG
    TATGCCAAGA AGATCATAGA GTGGCCTCCG TTTGAATACA TGATCCTGGC CACCATTATT
    GCTAACTGCA TCGTCCTAGC CTTGGAGCAG CACCTGCCTG GAGAAGACAA GACACCCATG
    GCTAAGAGAC TTGAAAAAAC AGAGCCCTAC TTCATAGGAA TGTTTTGCTT TGAAGCTGGA
    ATAAAAATCA TTGCTTTGGG ATTTGTATTC CACAAGGGCT CGTACCTGAG GAATGGTTGG
    AACGTCATGG ACTTTATTGT CGTATCGAGT GGGATTCTGG CCACAGCAGG AGCCCACATG
    AATATTTCAT TAGACCTGCG CACACTGAGG GCTGTGAGGG TATTACGGCC CCTCAAACTG
    GTCTCTGGCA TTCCTAGTCT GCAAATAGTT CTTAAATCCA TAATGAAGGC AATGGTGCCA
    TTGCTTCAGA TCGGACTGCT ACTGTTTTTT GCTATTCTAA TGTTTGCCAT TATTGGATTA
    GAGTTCTACA GTGGGAAACT CCACAAGCAA TGTGAACCTG TAGGTAAAGA TTCTGAGAGA
    GCAGACTCGC CTGAGAATGA ATTTCCATGT GGAAGCAAAC TGTGTCCTGA CCATTATAAC
    TGCTCTGGCT ACTGGACGGG ACCCAATGAT GGCATCACTC AGTTTGATAA CATCTTATTT
    TCTTTACTCA CCGTCTTCCA GTGCATCACC ATGGAAGGCT GGACGACCAT CCTCTATTAC
    ACCAACGATG CCTTAGGCTC TACTTGGAAT TGGCTCTACT TCATTCCTCT AATCATAATT
    GGCTCCTTCT TTGTGTTGAA TCTGGTATTA GGAGTCCTGT CAGGGGAGTT TGCTAAAGAG
    CGAGAGCGGG TAGAGAACCG GAGAGCCTTC ATGAAGCTTA GGAGACAGCA GCAGATTGAG
    AGGGAGCTGA ACGGATATCG GGCCTGGATC GACCGTGCAG AGGAGGTTAT GCTTGCAGAG
    GAGAACAAGA ATTCAGGACC TTCTGCTTTG GATGTGCTAA AAAGAGCTAC CACCATAAAG
    AGAAGAGGAG TGGATGATGC TCGAAGAGCT CCAGAAGAGC AGTATGCTGA CATCTCATCT
    CTAGGGATGC CACTGAACAG AGCCAGCATA CGCAGTGCTA AAAGAGGACC AACATCCTAT
    TTCCGACGGA AAGAGCGCCT GCTACGGATC TCCGTCCGCC GTATGGTGAA GACTCAGACG
    TTCTACTGGA CTGTTCTGGG TCTGGTAGCC CTCAACACTC TCTGTGTAGC CATCGTACAT
    CACAAACAGC CACAGTGGTT ATCTAGTCTC CTCTACTATG CAGAGTTTTT GTTTCTGGGT
    TTGTTTCTTA CCGAGATGTT TCTGAAGATG TATGGACTCG GACCCAGACT CTATTTTCAC
    TCCTCCTTCA ACTGCTTCGA TTGTGGGGTT ATAGTAGGCA GCATCTTTGA GGTGTTCTGG
    GGCTTCTTCA GACCTGGCAC ATCCTTTGGG ATCAGCGTTC TTCGTGCCTT GCGGTTGTTG
    AGAATTTTCA AGATAACCAA ATACTGGGCG TCCCTGAGGA ACCTGGTCGT GTCTCTGATG
    AACTCAATGA AGTCTATTAT CAGTTTGCTC TTCCTGCTTT TTCTCTTTAT TGTGGTCTTT
    GCACTTCTGG GCATGCAGCT CTTTGGTGGA AGGTTCATTT TTGATGAGTA CACTCCCACC
    AATTTTGACA CTTTCCCAGC AGCCATCATG ACAGTGTTTC AAATTCTGAC TGGTGAGGAC
    TGGAATGAAG TCATGTACAA TGGCATCCGG TCTCAAGGTG GCGTGAAATC TGGGATGTGG
    TCGTCTGTCT ACTTCATTGT TCTCACTCTG TTTGGAAATT ATACGCTGCT TAACGTCTTC
    TTGGCTATCG CTGTCGACAA TTTGGCCAAT GCCCAGGAGC TCACAAAGGA CGAAGAGGAG
    GAGGAAGAGG CGTTTAATCA GAAACATGCC CTGCAGAAAG CCAAGGAGGT TGGGCCCATG
    TCCTCTTTCA TGTCCTCAGA GAGGAGGAGG AGGCCCTACC TGTACAGGAA GCGTGCAATT
    CATCGGAGCC GTGCAGCCTA CTCCTTCGAG GATGCAGAGA GTCTGGCTGA CGCTCGTCCA
    TTCAATGCCT CTAACTCTTT CATGTCCAGG AGAGAGAGGA GGAGGAGGTT GACCATGTCA
    GTGTGGGAGC AGAGGACCAG CCAGCTGCGG CGGCACAGGC AGATGTCCAG CCGCGAGATC
    CTTTTCAGCA GCCCCAGTGA AGAACAGGAC GGACCACCAG GCTGCCCTCT CCACCGGAAA
    CCTGGACTAT CTCCATCTGG CAGCAAGCGC AGAGCCACTG AGACCACTTC AACCACAATC
    ATGCCAGAGG GTACCTTAGA GCCACCCATG CCTATGGACA TGCCGGTGGA TGAGCAAGCT
    CTGCCCATTT CTATTCCTGA ACCCCCTTTA GCCATTCCAA TTCCAGAGCC ACCAATGCCC
    GTGGACGCAT GCCTCCCTGA ACCCCCAGTT ACACTGAATC TGCCCTTAAC AGAGCCATCT
    GAATCAAAGA CTTCACCCGA GGGTGCTCTG GACATCAACA ACCATTGCCC TAGACTAAAT
    GGGAACAGGC GGCAGCGGGC TGTCCGTAAG CACAGACCTC CTGCTATGGG GGACATGCTG
    ACCCCTGATA TGGGGCCCAG GCCCCGACGG CCACGCCACC GCGAGCTTCA CACAGACCCC
    AGGGGAAAAG AGACTCTGCA AGGTGATGGT GTTGACGGTA AAGAACAAAA GCCAGGAAGT
    GAGGGTGATG AAAAGAGTGA TGGAGAAATC AGTCCAGATG GCAGAGGGCT TGAACAAAGG
    CAGAACAAAC CAGACAACAC AGAATCATCC ACAGAGTCCA AACCAGCAAA TTCCCCCCGC
    AAAATCCCAC CTCTTGACTC TGAACCTGCT ACTCTACAGC TCACTGACAA CACACTGCAG
    CAGTCTTCAC AGGGAACTAC ATGTGCCGGT GAGACCGATA ACAATAACCA CGGGTCCTTG
    AATCAGGATA AGTCCTCCCT CAACACCAGC ATCGTGATAG AGGTCACCGA CACTCAGCCA
    TCCATGGAGC TGACCTCCAT TACGGGTAAT AGTAAAGATG TGGAGGCCTC AAAGCCAGAG
    AAAGAAGAAG AAACCCCAGA TGCACCCAAA GGGCCTCCAC CTGTCAGCAT GTTCATCTTC
    AAACCCAACA ACCCGGTCCG CAGGGCCTGC CACTACGTTG TCAACCTTCG TTATTTTGAG
    ATGTCAATCC TTCTTGTGAT CGCCGCCAGC AGTATCGCAT TGGCTGCCGA AGACCCCGTC
    AACACTAATT CGGACAGGAA CAAAGTTCTG CGCTATTTTG ACTATGTATT CACTGGGGTC
    TTCACGTTTG AAATGATAAT CAAGATGATT GATCAGGGGT TGGTCTTACA CTATGGCTCC
    TATTTTCGTG ACCCGTGGAA CATTCTGGAT TTCATAGTTG TGGTGGGAGC TCTGGTGGCT
    TTTGCTCTCA CGAATGTGAT GGGGAGTAAC ACAGGAAGAG ACATTAAAAC CATTAAATCG
    CTGCGTGTAC TCCGAGTTCT GAGGCCTCTC AAGACGATTA AAAGACTTCC TAAACTCAAG
    GCTGTGTTTG ACTGTGTGGT AACATCACTG AAGAATGTTT TCAACATCCT CATCGTCTAC
    AAGCTGTTTA TGTTCATATT TGCCGTCATT GCCGTGCAGC TTTTCAAAGG GAAATTTTAC
    TACTGTACCG ACAGTTCCAA GGACACTGAA AACGAGTGCA AAGGCTACTA CATTGACTAT
    GATAAAGACA AGAAAAAGGA GAAACGAGAG TGGAAGAGAC GCGATTTCCA TTATGATAAC
    GTTGTCTGGG CTCTGTTGAC TCTCTTCACC GTATCCACTG GCGAAGGCTG GCCGCAAGTT
    CTGCAACACT CTGTGGATGT GACAGAGGAG GACCGGGGTC CCAGTCAGGG GAACAGGATG
    GAGATGTCTA TCTTCTATGT CATTTATTTT GTGGTGTTCC CCTTCTTCTT CGTCAACATA
    TTTGTGGCCC TCATCATCAT CACTTTCCAA GAACAGGGTG ACAAAATGAT GGAGGAGTGT
    AGTCTGGAGA AAAACGAGAG GGCTTGTATC GACTTTGCCA TCAGTGCCAA ACCTTTGACT
    CGCTACATGC CCCAGAACAG ACAGACCTTA CAGTACAGAC TGTGGCAATT TGTGGTGTCG
    CCCTCATTTG AGTACACAGT ACTGACAATG ATCGCCCTTA ACACCATCGT TCTCATGATG
    AAGCATCATG ATCCTCGTCT TGAAGGCTAT GACGATGTGC TCAAATATCT CAACACAATT
    TTCACCGTTA TTTTCTCTGT TGAGTGTGTT CTCAAGATCC TGGCTTTTGG TATAATGAAC
    TATTTCAGAG ACACATGGAA TATATTTGAC TTCATCACGG TTCTAGGAAG CATCACAGAA
    ATTCTGGTGG ACAAATTTCA GACTGGAAAA ACTTTTAATA TGGGCTTCCT GAAGCTATTC
    CGGGCAGCTC GGCTGATTAA ACTCCTGCGT CAGGGCTACA CCATCCGTAT TCTGCTCTGG
    ACTTTTGTTC AGTCCTTCAA GGCTCTACCA TATGTCTGTC TGCTCATCGC CATGCTGTTT
    TTCATCTATG CCATCATTGG CATGCAGGTA TTTGGCAACA TCAAGATAAA TGAGAAAACC
    CAAATCAATG AACACAATAA CTTCAGGACT TTCTTTGGTG CACTCATGCT TTTGTTCAGG
    AGTGCAACAG GTGAATCATG GCAGGAAATC ATGCTATCGT GTTTAGGAGA GAAGGAGTGT
    GAAATTGACA CTTCTGTCAA CTCAACAACA AAGACAATAA AAAACGACTG CGGTACCGAC
    TTTGCCTACT TCTACTTTGT CTCCTTCATC TTCTTCAGTT CTTTTCTGAT GCTGAATCTG
    TTTGTGGCTG TGATCATGGA TAATTTTGAG TACCTGACAC GTGACTCATC CATTCTGGGT
    CCTCACCACT TAGATGAGTT TGTACGGATC TGGGGAGAGT ATGACCGTGC TGCATGTGGC
    CGTATTCATT ACACTGATAT GTACGAGATG CTGACCTGCA TGTCGCCACC GCTTGGCCTG
    GGCAAGAAAT GTCCCTCCAA AGTGGCGTAT AAGCGGTTGG TTCTGATGAA CATGCCAGTG
    GATGAAGACA TGTCTGTCCA CTTCACCTCC ACACTTATGG CCCTCATACG CACCGCACTG
    GACATTAAAA TTGCTCGAGG AGGGGAAGAC AGGGGTCAGA TGGATGTTGA ACTGCAGAAG
    GAAATCAGTG TCATCTGGCC GCATCTCTCA CAGAAATCTC TTGATCTGCT TGTACCCATT
    CACAAAGCTA GTGACATGAC CATTGGGAAG ATCTATGCAG CCATGATGAT CATGGACTAC
    TACAAGCAGA ACAAAGCCAA GAAGCTCCGT CAGCAGCTCG AGGAACAGAA ACACGCACCA
    ATGTTCCAAC GCATGGATGC ATCCTCGCTG CCGCAGGACA TCCTCTGCAG TGCCAAGGCT
    TTGCCATTCC TGACTCACAG TGCAGGATCT GCCCTCACTA GAGGTGGGTT TGTGGCACTG
    AGTCCTATCT CTCCTCAAGA TCTCTTCATG CAGCCCATGA CCCAAGACAG CATGGAGGAG
    AGGGAGGAAT ACCAACACCC GTATCACCTG CAAAGCATGG TGCCTGAGCA CAAAGAATTT
    AGCAGTGTGA TGTGCCCCAC TAGCATGCAG GCTGATCCAC TGCAAGTGCC AGGAAGAGGC
    GTCTCTATCC GAGCCTCTTC CATGCCTCGC CTGGCGGTAG AGCCACAGGC CTCTTTAGAG
    AACAAACTGA TGAAGCGCTC ATTCTCCACC ATCGGGGATC AGTGTGTAAA TGGTGACTGG
    CAGGAAGAGT TCTCTCTGGA GAGGGTGAGT CCTGACCGGC TGGAGAGACC CAGGCAAAGA
    AGCTTCAGAG GTGTCAGGTC AAACCTGCGG GAGCCAGTGG ACAAAGAGAG AGGACGGTCT
    AAAGAACGAA GACACCTGCT GTCTCCAGAT GTGTCTCGCT GCAACTCAGA GGAAAGGAGT
    CAGTCTCAGG AGAGACGTCA TTCTCCATCG CCCAGTGAGG GGAGGAAGCA CACAGCTCTC
    AGACAGGGAA ATAATACAGG CAGCCCTATT GCCTCCACGT CTGATTCAGG GACGCCTCGA
    GGACGCCGCC AACTCCCACA GACACCCTCT CGCCCTCGGC CTCATATCTC CTACTCTCCT
    TTAGTGTGCC GTGCCCATCC AGCACCAGGG GATGATCGGC CACGAGCTCC ACCCGCAGAG
    GAAGGAGAGG AGCCCACTGC AACCGGCCCA GGCACCGCCA TGGCCCACTC TACCCCTCAC
    CGCTACATCT CAGAGCCCTA CCTGCCACTG CAGGAGGACG TCAACAGTCC AGACTCCAGT
    GGTGTGGAGG AGACTCTCAC TTTTGAGGCT GCAATAGCAA CCAGCCTGGG CCGCTCTAAT
    ACCATTAGCT CGGCGCCACA GCTACGTCAC TCCTGGCAGG TGCCTAATGG ACATTTCCGC
    AAACGACTAG CACAAGCCAC AGCTGGGACG TCCGGCTGTG AGCTTCTCAG TGACACCGAT
    GAGGATGACC GCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212913.2
    CDS357..7214
    Translation

    Target ORF information:

    RefSeq Version XM_017357424.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357424.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCTCGCT TCGGAGACGA TGTTCCCGGT CTGCTGGGAG CCGGGGATGG GGGTTCGGAG 
    CGCAGTCGGA ACCGGGATGC TCCGGCGGTA TCCACAGGTG GAGTTTCACC AGCCTTCAAA
    CAGACCAAAG CGCAACGAGC CCGAACCATG GCTCTGTACA ACCCCATTCC TGTCCGACAG
    AACTGCTTCA CCGTCAACAG ATCTCTCTTC ATCTTTGGAG AGGACAACAT CGTTAGGAAG
    TATGCCAAGA AGATCATAGA GTGGCCTCCG TTTGAATACA TGATCCTGGC CACCATTATT
    GCTAACTGCA TCGTCCTAGC CTTGGAGCAG CACCTGCCTG GAGAAGACAA GACACCCATG
    GCTAAGAGAC TTGAAAAAAC AGAGCCCTAC TTCATAGGAA TGTTTTGCTT TGAAGCTGGA
    ATAAAAATCA TTGCTTTGGG ATTTGTATTC CACAAGGGCT CGTACCTGAG GAATGGTTGG
    AACGTCATGG ACTTTATTGT CGTATCGAGT GGGATTCTGG CCACAGCAGG AGCCCACATG
    AATATTTCAT TAGACCTGCG CACACTGAGG GCTGTGAGGG TATTACGGCC CCTCAAACTG
    GTCTCTGGCA TTCCTAGTCT GCAAATAGTT CTTAAATCCA TAATGAAGGC AATGGTGCCA
    TTGCTTCAGA TCGGACTGCT ACTGTTTTTT GCTATTCTAA TGTTTGCCAT TATTGGATTA
    GAGTTCTACA GTGGGAAACT CCACAAGCAA TGTGAACCTG TAGGTAAAGA TTCTGAGAGA
    GCAGACTCGC CTGAGAATGA ATTTCCATGT GGAAGCAAAC TGTGTCCTGA CCATTATAAC
    TGCTCTGGCT ACTGGACGGG ACCCAATGAT GGCATCACTC AGTTTGATAA CATCTTATTT
    TCTTTACTCA CCGTCTTCCA GTGCATCACC ATGGAAGGCT GGACGACCAT CCTCTATTAC
    ACCAACGATG CCTTAGGCTC TACTTGGAAT TGGCTCTACT TCATTCCTCT AATCATAATT
    GGCTCCTTCT TTGTGTTGAA TCTGGTATTA GGAGTCCTGT CAGGGGAGTT TGCTAAAGAG
    CGAGAGCGGG TAGAGAACCG GAGAGCCTTC ATGAAGCTTA GGAGACAGCA GCAGATTGAG
    AGGGAGCTGA ACGGATATCG GGCCTGGATC GACCGTGCAG AGGAGGTTAT GCTTGCAGAG
    GAGAACAAGA ATTCAGGACC TTCTGCTTTG GATGTGCTAA AAAGAGCTAC CACCATAAAG
    AGAAGAGGAG TGGATGATGC TCGAAGAGCT CCAGAAGAGC AGTATGCTGA CATCTCATCT
    CTAGGGATGC CACTGAACAG AGCCAGCATA CGCAGTGCTA AAAGAGGACC AACATCCTAT
    TTCCGACGGA AAGAGCGCCT GCTACGGATC TCCGTCCGCC GTATGGTGAA GACTCAGACG
    TTCTACTGGA CTGTTCTGGG TCTGGTAGCC CTCAACACTC TCTGTGTAGC CATCGTACAT
    CACAAACAGC CACAGTGGTT ATCTAGTCTC CTCTACTATG CAGAGTTTTT GTTTCTGGGT
    TTGTTTCTTA CCGAGATGTT TCTGAAGATG TATGGACTCG GACCCAGACT CTATTTTCAC
    TCCTCCTTCA ACTGCTTCGA TTGTGGGGTT ATAGTAGGCA GCATCTTTGA GGTGTTCTGG
    GGCTTCTTCA GACCTGGCAC ATCCTTTGGG ATCAGCGTTC TTCGTGCCTT GCGGTTGTTG
    AGAATTTTCA AGATAACCAA ATACTGGGCG TCCCTGAGGA ACCTGGTCGT GTCTCTGATG
    AACTCAATGA AGTCTATTAT CAGTTTGCTC TTCCTGCTTT TTCTCTTTAT TGTGGTCTTT
    GCACTTCTGG GCATGCAGCT CTTTGGTGGA AGGTTCATTT TTGATGAGTA CACTCCCACC
    AATTTTGACA CTTTCCCAGC AGCCATCATG ACAGTGTTTC AAATTCTGAC TGGTGAGGAC
    TGGAATGAAG TCATGTACAA TGGCATCCGG TCTCAAGGTG GCGTGAAATC TGGGATGTGG
    TCGTCTGTCT ACTTCATTGT TCTCACTCTG TTTGGAAATT ATACGCTGCT TAACGTCTTC
    TTGGCTATCG CTGTCGACAA TTTGGCCAAT GCCCAGGAGC TCACAAAGGA CGAAGAGGAG
    GAGGAAGAGG CGTTTAATCA GAAACATGCC CTGCAGAAAG CCAAGGAGGT TGGGCCCATG
    TCCTCTTTCA TGTCCTCAGA GAGGAGGAGG AGGCCCTACC TGTACAGGAA GCGTGCAATT
    CATCGGAGCC GTGCAGCCTA CTCCTTCGAG GATGCAGAGA GTCTGGCTGA CGCTCGTCCA
    TTCAATGCCT CTAACTCTTT CATGTCCAGG AGAGAGAGGA GGAGGAGGTT GACCATGTCA
    GTGTGGGAGC AGAGGACCAG CCAGCTGCGG CGGCACAGGC AGATGTCCAG CCGCGAGATC
    CTTTTCAGCA GCCCCAGTGA AGAACAGGAC GGACCACCAG GCTGCCCTCT CCACCGGAAA
    CCTGGACTAT CTCCATCTGG CAGCAAGCGC AGAGCCACTG AGACCACTTC AACCACAATC
    ATGCCAGAGG GTACCTTAGA GCCACCCATG CCTATGGACA TGCCGGTGGA TGAGCAAGCT
    CTGCCCATTT CTATTCCTGA ACCCCCTTTA GCCATTCCAA TTCCAGAGCC ACCAATGCCC
    GTGGACGCAT GCCTCCCTGA ACCCCCAGTT ACACTGAATC TGCCCTTAAC AGAGCCATCT
    GAATCAAAGA CTTCACCCGA GGGTGCTCTG GACATCAACA ACCATTGCCC TAGACTAAAT
    GGGAACAGGC GGCAGCGGGC TGTCCGTAAG CACAGACCTC CTGCTATGGG GGACATGCTG
    ACCCCTGATA TGGGGCCCAG GCCCCGACGG CCACGCCACC GCGAGCTTCA CACAGACCCC
    AGGGGAAAAG AGACTCTGCA AGGTGATGGT GTTGACGGTA AAGAACAAAA GCCAGGAAGT
    GAGGGTGATG AAAAGAGTGA TGGAGAAATC AGTCCAGATG GCAGAGGGCT TGAACAAAGG
    CAGAACAAAC CAGACAACAC AGAATCATCC ACAGAGTCCA AACCAGCAAA TTCCCCCCGC
    AAAATCCCAC CTCTTGACTC TGAACCTGCT ACTCTACAGC TCACTGACAA CACACTGCAG
    CAGTCTTCAC AGGGAACTAC ATGTGCCGGT GAGACCGATA ACAATAACCA CGGGTCCTTG
    AATCAGGATA AGTCCTCCCT CAACACCAGC ATCGTGATAG AGGTCACCGA CACTCAGCCA
    TCCATGGAGC TGACCTCCAT TACGGGTAAT AGTAAAGATG TGGAGGCCTC AAAGCCAGAG
    AAAGAAGAAG AAACCCCAGA TGCACCCAAA GGGCCTCCAC CTGTCAGCAT GTTCATCTTC
    AAACCCAACA ACCCGGTCCG CAGGGCCTGC CACTACGTTG TCAACCTTCG TTATTTTGAG
    ATGTCAATCC TTCTTGTGAT CGCCGCCAGC AGTATCGCAT TGGCTGCCGA AGACCCCGTC
    AACACTAATT CGGACAGGAA CAAAGTTCTG CGCTATTTTG ACTATGTATT CACTGGGGTC
    TTCACGTTTG AAATGATAAT CAAGATGATT GATCAGGGGT TGGTCTTACA CTATGGCTCC
    TATTTTCGTG ACCCGTGGAA CATTCTGGAT TTCATAGTTG TGGTGGGAGC TCTGGTGGCT
    TTTGCTCTCA CGAATGTGAT GGGGAGTAAC ACAGGAAGAG ACATTAAAAC CATTAAATCG
    CTGCGTGTAC TCCGAGTTCT GAGGCCTCTC AAGACGATTA AAAGACTTCC TAAACTCAAG
    GCTGTGTTTG ACTGTGTGGT AACATCACTG AAGAATGTTT TCAACATCCT CATCGTCTAC
    AAGCTGTTTA TGTTCATATT TGCCGTCATT GCCGTGCAGC TTTTCAAAGG GAAATTTTAC
    TACTGTACCG ACAGTTCCAA GGACACTGAA AACGAGTGCA AAGGCTACTA CATTGACTAT
    GATAAAGACA AGAAAAAGGA GAAACGAGAG TGGAAGAGAC GCGATTTCCA TTATGATAAC
    GTTGTCTGGG CTCTGTTGAC TCTCTTCACC GTATCCACTG GCGAAGGCTG GCCGCAAGTT
    CTGCAACACT CTGTGGATGT GACAGAGGAG GACCGGGGTC CCAGTCAGGG GAACAGGATG
    GAGATGTCTA TCTTCTATGT CATTTATTTT GTGGTGTTCC CCTTCTTCTT CGTCAACATA
    TTTGTGGCCC TCATCATCAT CACTTTCCAA GAACAGGGTG ACAAAATGAT GGAGGAGTGT
    AGTCTGGAGA AAAACGAGAG GGCTTGTATC GACTTTGCCA TCAGTGCCAA ACCTTTGACT
    CGCTACATGC CCCAGAACAG ACAGACCTTA CAGTACAGAC TGTGGCAATT TGTGGTGTCG
    CCCTCATTTG AGTACACAGT ACTGACAATG ATCGCCCTTA ACACCATCGT TCTCATGATG
    AAGCATCATG ATCCTCGTCT TGAAGGCTAT GACGATGTGC TCAAATATCT CAACACAATT
    TTCACCGTTA TTTTCTCTGT TGAGTGTGTT CTCAAGATCC TGGCTTTTGG TATAATGAAC
    TATTTCAGAG ACACATGGAA TATATTTGAC TTCATCACGG TTCTAGGAAG CATCACAGAA
    ATTCTGGTGG ACAAATTTCA GACTGGAAAA ACTTTTAATA TGGGCTTCCT GAAGCTATTC
    CGGGCAGCTC GGCTGATTAA ACTCCTGCGT CAGGGCTACA CCATCCGTAT TCTGCTCTGG
    ACTTTTGTTC AGTCCTTCAA GGCTCTACCA TATGTCTGTC TGCTCATCGC CATGCTGTTT
    TTCATCTATG CCATCATTGG CATGCAGGTA TTTGGCAACA TCAAGATAAA TGAGAAAACC
    CAAATCAATG AACACAATAA CTTCAGGACT TTCTTTGGTG CACTCATGCT TTTGTTCAGG
    AGTGCAACAG GTGAATCATG GCAGGAAATC ATGCTATCGT GTTTAGGAGA GAAGGAGTGT
    GAAATTGACA CTTCTGTCAA CTCAACAACA AAGACAATAA AAAACGACTG CGGTACCGAC
    TTTGCCTACT TCTACTTTGT CTCCTTCATC TTCTTCAGTT CTTTTCTGAT GCTGAATCTG
    TTTGTGGCTG TGATCATGGA TAATTTTGAG TACCTGACAC GTGACTCATC CATTCTGGGT
    CCTCACCACT TAGATGAGTT TGTACGGATC TGGGGAGAGT ATGACCGTGC TGCATGTGGC
    CGTATTCATT ACACTGATAT GTACGAGATG CTGACCTGCA TGTCGCCACC GCTTGGCCTG
    GGCAAGAAAT GTCCCTCCAA AGTGGCGTAT AAGCGGTTGG TTCTGATGAA CATGCCAGTG
    GATGAAGACA TGTCTGTCCA CTTCACCTCC ACACTTATGG CCCTCATACG CACCGCACTG
    GACATTAAAA TTGCTCGAGG AGGGGAAGAC AGGGGTCAGA TGGATGTTGA ACTGCAGAAG
    GAAATCAGTG TCATCTGGCC GCATCTCTCA CAGAAATCTC TTGATCTGCT TGTACCCATT
    CACAAAGCTA GTGACATGAC CATTGGGAAG ATCTATGCAG CCATGATGAT CATGGACTAC
    TACAAGCAGA ACAAAGCCAA GAAGCTCCGT CAGCAGCTCG AGGAACAGAA ACACGCACCA
    ATGTTCCAAC GCATGGATGC ATCCTCGCTG CCGCAGGACA TCCTCTGCAG TGCCAAGGCT
    TTGCCATTCC TGACTCACAG TGCAGGATCT GCCCTCACTA GAGGTGGGTT TGTGGCACTG
    AGTCCTATCT CTCCTCAAGA TCTCTTCATG CAGCCCATGA CCCAAGACAG CATGGAGGAG
    AGGGAGGAAT ACCAACACCC GTATCACCTG CAAAGCATGG TGCCTGAGCA CAAAGAATTT
    AGCAGTGTGA TGTGCCCCAC TAGCATGCAG GCTGATCCAC TGCAAGTGCC AGGAAGAGGC
    GTCTCTATCC GAGCCTCTTC CATGCCTCGC CTGGCGGTAG AGCCACAGGC CTCTTTAGAG
    AACAAACTGA TGAAGCGCTC ATTCTCCACC ATCGGGGATC AGTGTGTAAA TGGTGACTGG
    CAGGAAGAGT TCTCTCTGGA GAGGGTGAGT CCTGACCGGC TGGAGAGACC CAGGCAAAGA
    AGCTTCAGAG GTGTCAGGTC AAACCTGCGG GAGCCAGTGG ACAAAGAGAG AGGACGGTCT
    AAAGAACGAA GACACCTGCT GTCTCCAGAT GTGTCTCGCT GCAACTCAGA GGAAAGGAGT
    CAGTCTCAGG AGAGACGTCA TTCTCCATCG CCCAGTGAGG GGAGGAAGCA CACAGCTCTC
    AGACAGGGAA ATAATACAGG CAGCCCTATT GCCTCCACGT CTGATTCAGG GACGCCTCGA
    GGACGCCGCC AACTCCCACA GACACCCTCT CGCCCTCGGC CTCATATCTC CTACTCTCCT
    TTAGTGTGCC GTGCCCATCC AGCACCAGGG GATGATCGGC CACGAGCTCC ACCCGCAGAG
    GAAGGAGAGG AGCCCACTGC AACCGGCCCA GGCACCGCCA TGGCCCACTC TACCCCTCAC
    CGCTACATCT CAGAGCCCTA CCTGCCACTG CAGGAGGACG TCAACAGTCC AGACTCCAGT
    GGTGTGGAGG AGACTCTCAC TTTTGAGGCT GCAATAGCAA CCAGCCTGGG CCGCTCTAAT
    ACCATTAGCT CGGCGCCACA GCTACGTCAC TCCTGGCAGG TGCCTAATGG ACATTTCCGC
    AAACGACTAG CACAAGCCAC AGCTGGGACG TCCGGCTGTG AGCTTCTCAG TGACACCGAT
    GAGGATGACC GCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60028
    Clone ID Related Accession (Same CDS sequence) XM_017357424.1
    Accession Version XM_017357424.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGATCCTGG CCACCATTAT TGCTAACTGC ATCGTCCTAG CCTTGGAGCA GCACCTGCCT 
    GGAGAAGACA AGACACCCAT GGCTAAGAGA CTTGAAAAAA CAGAGCCCTA CTTCATAGGA
    ATGTTTTGCT TTGAAGCTGG AATAAAAATC ATTGCTTTGG GATTTGTATT CCACAAGGGC
    TCGTACCTGA GGAATGGTTG GAACGTCATG GACTTTATTG TCGTATCGAG TGGGATTCTG
    GCCACAGCAG GAGCCCACAT GAATATTTCA TTAGACCTGC GCACACTGAG GGCTGTGAGG
    GTATTACGGC CCCTCAAACT GGTCTCTGGC ATTCCTAGTC TGCAAATAGT TCTTAAATCC
    ATAATGAAGG CAATGGTGCC ATTGCTTCAG ATCGGACTGC TACTGTTTTT TGCTATTCTA
    ATGTTTGCCA TTATTGGATT AGAGTTCTAC AGTGGGAAAC TCCACAAGCA ATGTGAACCT
    GTAGGTAAAG ATTCTGAGAG AGCAGACTCG CCTGAGAATG AATTTCCATG TGGAAGCAAA
    CTGTGTCCTG ACCATTATAA CTGCTCTGGC TACTGGACGG GACCCAATGA TGGCATCACT
    CAGTTTGATA ACATCTTATT TTCTTTACTC ACCGTCTTCC AGTGCATCAC CATGGAAGGC
    TGGACGACCA TCCTCTATTA CACCAACGAT GCCTTAGGCT CTACTTGGAA TTGGCTCTAC
    TTCATTCCTC TAATCATAAT TGGCTCCTTC TTTGTGTTGA ATCTGGTATT AGGAGTCCTG
    TCAGGGGAGT TTGCTAAAGA GCGAGAGCGG GTAGAGAACC GGAGAGCCTT CATGAAGCTT
    AGGAGACAGC AGCAGATTGA GAGGGAGCTG AACGGATATC GGGCCTGGAT CGACCGTGCA
    GAGGAGGTTA TGCTTGCAGA GGAGAACAAG AATTCAGGAC CTTCTGCTTT GGATGTGCTA
    AAAAGAGCTA CCACCATAAA GAGAAGAGGA GTGGATGATG CTCGAAGAGC TCCAGAAGAG
    CAGTATGCTG ACATCTCATC TCTAGGGATG CCACTGAACA GAGCCAGCAT ACGCAGTGCT
    AAAAGAGGAC CAACATCCTA TTTCCGACGG AAAGAGCGCC TGCTACGGAT CTCCGTCCGC
    CGTATGGTGA AGACTCAGAC GTTCTACTGG ACTGTTCTGG GTCTGGTAGC CCTCAACACT
    CTCTGTGTAG CCATCGTACA TCACAAACAG CCACAGTGGT TATCTAGTCT CCTCTACTAT
    GCAGAGTTTT TGTTTCTGGG TTTGTTTCTT ACCGAGATGT TTCTGAAGAT GTATGGACTC
    GGACCCAGAC TCTATTTTCA CTCCTCCTTC AACTGCTTCG ATTGTGGGGT TATAGTAGGC
    AGCATCTTTG AGGTGTTCTG GGGCTTCTTC AGACCTGGCA CATCCTTTGG GATCAGCGTT
    CTTCGTGCCT TGCGGTTGTT GAGAATTTTC AAGATAACCA AATACTGGGC GTCCCTGAGG
    AACCTGGTCG TGTCTCTGAT GAACTCAATG AAGTCTATTA TCAGTTTGCT CTTCCTGCTT
    TTTCTCTTTA TTGTGGTCTT TGCACTTCTG GGCATGCAGC TCTTTGGTGG AAGGTTCATT
    TTTGATGAGT ACACTCCCAC CAATTTTGAC ACTTTCCCAG CAGCCATCAT GACAGTGTTT
    CAAATTCTGA CTGGTGAGGA CTGGAATGAA GTCATGTACA ATGGCATCCG GTCTCAAGGT
    GGCGTGAAAT CTGGGATGTG GTCGTCTGTC TACTTCATTG TTCTCACTCT GTTTGGAAAT
    TATACGCTGC TTAACGTCTT CTTGGCTATC GCTGTCGACA ATTTGGCCAA TGCCCAGGAG
    CTCACAAAGG ACGAAGAGGA GGAGGAAGAG GCGTTTAATC AGAAACATGC CCTGCAGAAA
    GCCAAGGAGG TTGGGCCCAT GTCCTCTTTC ATGTCCTCAG AGAGGAGGAG GAGGCCCTAC
    CTGTACAGGA AGCGTGCAAT TCATCGGAGC CGTGCAGCCT ACTCCTTCGA GGATGCAGAG
    AGTCTGGCTG ACGCTCGTCC ATTCAATGCC TCTAACTCTT TCATGTCCAG GAGAGAGAGG
    AGGAGGAGGT TGACCATGTC AGTGTGGGAG CAGAGGACCA GCCAGCTGCG GCGGCACAGG
    CAGATGTCCA GCCGCGAGAT CCTTTTCAGC AGCCCCAGTG AAGAACAGGA CGGACCACCA
    GGCTGCCCTC TCCACCGGAA ACCTGGACTA TCTCCATCTG GCAGCAAGCG CAGAGCCACT
    GAGACCACTT CAACCACAAT CATGCCAGAG GGTACCTTAG AGCCACCCAT GCCTATGGAC
    ATGCCGGTGG ATGAGCAAGC TCTGCCCATT TCTATTCCTG AACCCCCTTT AGCCATTCCA
    ATTCCAGAGC CACCAATGCC CGTGGACGCA TGCCTCCCTG AACCCCCAGT TACACTGAAT
    CTGCCCTTAA CAGAGCCATC TGAATCAAAG ACTTCACCCG AGGGTGCTCT GGACATCAAC
    AACCATTGCC CTAGACTAAA TGGGAACAGG CGGCAGCGGG CTGTCCGTAA GCACAGACCT
    CCTGCTATGG GGGACATGCT GACCCCTGAT ATGGGGCCCA GGCCCCGACG GCCACGCCAC
    CGCGAGCTTC ACACAGACCC CAGGGGAAAA GAGACTCTGC AAGGTGATGG TGTTGACGGT
    AAAGAACAAA AGCCAGGAAG TGAGGGTGAT GAAAAGAGTG ATGGAGAAAT CAGTCCAGAT
    GGCAGAGGGC TTGAACAAAG GCAGAACAAA CCAGACAACA CAGAATCATC CACAGAGTCC
    AAACCAGCAA ATTCCCCCCG CAAAATCCCA CCTCTTGACT CTGAACCTGC TACTCTACAG
    CTCACTGACA ACACACTGCA GCAGTCTTCA CAGGGAACTA CATGTGCCGG TGAGACCGAT
    AACAATAACC ACGGGTCCTT GAATCAGGAT AAGTCCTCCC TCAACACCAG CATCGTGATA
    GAGGTCACCG ACACTCAGCC ATCCATGGAG CTGACCTCCA TTACGGGTAA TAGTAAAGAT
    GTGGAGGCCT CAAAGCCAGA GAAAGAAGAA GAAACCCCAG ATGCACCCAA AGGGCCTCCA
    CCTGTCAGCA TGTTCATCTT CAAACCCAAC AACCCGGTCC GCAGGGCCTG CCACTACGTT
    GTCAACCTTC GTTATTTTGA GATGTCAATC CTTCTTGTGA TCGCCGCCAG CAGTATCGCA
    TTGGCTGCCG AAGACCCCGT CAACACTAAT TCGGACAGGA ACAAAGTTCT GCGCTATTTT
    GACTATGTAT TCACTGGGGT CTTCACGTTT GAAATGATAA TCAAGATGAT TGATCAGGGG
    TTGGTCTTAC ACTATGGCTC CTATTTTCGT GACCCGTGGA ACATTCTGGA TTTCATAGTT
    GTGGTGGGAG CTCTGGTGGC TTTTGCTCTC ACGAATGTGA TGGGGAGTAA CACAGGAAGA
    GACATTAAAA CCATTAAATC GCTGCGTGTA CTCCGAGTTC TGAGGCCTCT CAAGACGATT
    AAAAGACTTC CTAAACTCAA GGCTGTGTTT GACTGTGTGG TAACATCACT GAAGAATGTT
    TTCAACATCC TCATCGTCTA CAAGCTGTTT ATGTTCATAT TTGCCGTCAT TGCCGTGCAG
    CTTTTCAAAG GGAAATTTTA CTACTGTACC GACAGTTCCA AGGACACTGA AAACGAGTGC
    AAAGGCTACT ACATTGACTA TGATAAAGAC AAGAAAAAGG AGAAACGAGA GTGGAAGAGA
    CGCGATTTCC ATTATGATAA CGTTGTCTGG GCTCTGTTGA CTCTCTTCAC CGTATCCACT
    GGCGAAGGCT GGCCGCAAGT TCTGCAACAC TCTGTGGATG TGACAGAGGA GGACCGGGGT
    CCCAGTCAGG GGAACAGGAT GGAGATGTCT ATCTTCTATG TCATTTATTT TGTGGTGTTC
    CCCTTCTTCT TCGTCAACAT ATTTGTGGCC CTCATCATCA TCACTTTCCA AGAACAGGGT
    GACAAAATGA TGGAGGAGTG TAGTCTGGAG AAAAACGAGA GGGCTTGTAT CGACTTTGCC
    ATCAGTGCCA AACCTTTGAC TCGCTACATG CCCCAGAACA GACAGACCTT ACAGTACAGA
    CTGTGGCAAT TTGTGGTGTC GCCCTCATTT GAGTACACAG TACTGACAAT GATCGCCCTT
    AACACCATCG TTCTCATGAT GAAGCATCAT GATCCTCGTC TTGAAGGCTA TGACGATGTG
    CTCAAATATC TCAACACAAT TTTCACCGTT ATTTTCTCTG TTGAGTGTGT TCTCAAGATC
    CTGGCTTTTG GTATAATGAA CTATTTCAGA GACACATGGA ATATATTTGA CTTCATCACG
    GTTCTAGGAA GCATCACAGA AATTCTGGTG GACAAATTTC AGACTGGAAA AACTTTTAAT
    ATGGGCTTCC TGAAGCTATT CCGGGCAGCT CGGCTGATTA AACTCCTGCG TCAGGGCTAC
    ACCATCCGTA TTCTGCTCTG GACTTTTGTT CAGTCCTTCA AGGCTCTACC ATATGTCTGT
    CTGCTCATCG CCATGCTGTT TTTCATCTAT GCCATCATTG GCATGCAGGT ATTTGGCAAC
    ATCAAGATAA ATGAGAAAAC CCAAATCAAT GAACACAATA ACTTCAGGAC TTTCTTTGGT
    GCACTCATGC TTTTGTTCAG GAGTGCAACA GGTGAATCAT GGCAGGAAAT CATGCTATCG
    TGTTTAGGAG AGAAGGAGTG TGAAATTGAC ACTTCTGTCA ACTCAACAAC AAAGACAATA
    AAAAACGACT GCGGTACCGA CTTTGCCTAC TTCTACTTTG TCTCCTTCAT CTTCTTCAGT
    TCTTTTCTGA TGCTGAATCT GTTTGTGGCT GTGATCATGG ATAATTTTGA GTACCTGACA
    CGTGACTCAT CCATTCTGGG TCCTCACCAC TTAGATGAGT TTGTACGGAT CTGGGGAGAG
    TATGACCGTG CTGCATGTGG CCGTATTCAT TACACTGATA TGTACGAGAT GCTGACCTGC
    ATGTCGCCAC CGCTTGGCCT GGGCAAGAAA TGTCCCTCCA AAGTGGCGTA TAAGCGGTTG
    GTTCTGATGA ACATGCCAGT GGATGAAGAC ATGTCTGTCC ACTTCACCTC CACACTTATG
    GCCCTCATAC GCACCGCACT GGACATTAAA ATTGCTCGAG GAGGGGAAGA CAGGGGTCAG
    ATGGATGTTG AACTGCAGAA GGAAATCAGT GTCATCTGGC CGCATCTCTC ACAGAAATCT
    CTTGATCTGC TTGTACCCAT TCACAAAGCT AGTGACATGA CCATTGGGAA GATCTATGCA
    GCCATGATGA TCATGGACTA CTACAAGCAG AACAAAGCCA AGAAGCTCCG TCAGCAGCTC
    GAGGAACAGA AACACGCACC AATGTTCCAA CGCATGGATG CATCCTCGCT GCCGCAGGAC
    ATCCTCTGCA GTGCCAAGGC TTTGCCATTC CTGACTCACA GTGCAGGATC TGCCCTCACT
    AGAGGTGGGT TTGTGGCACT GAGTCCTATC TCTCCTCAAG ATCTCTTCAT GCAGCCCATG
    ACCCAAGACA GCATGGAGGA GAGGGAGGAA TACCAACACC CGTATCACCT GCAAAGCATG
    GTGCCTGAGC ACAAAGAATT TAGCAGTGTG ATGTGCCCCA CTAGCATGCA GGCTGATCCA
    CTGCAAGTGC CAGGAAGAGG CGTCTCTATC CGAGCCTCTT CCATGCCTCG CCTGGCGGTA
    GAGCCACAGG CCTCTTTAGA GAACAAACTG ATGAAGCGCT CATTCTCCAC CATCGGGGAT
    CAGTGTGTAA ATGGTGACTG GCAGGAAGAG TTCTCTCTGG AGAGGGTGAG TCCTGACCGG
    CTGGAGAGAC CCAGGCAAAG AAGCTTCAGA GGTGTCAGGT CAAACCTGCG GGAGCCAGTG
    GACAAAGAGA GAGGACGGTC TAAAGAACGA AGACACCTGC TGTCTCCAGA TGTGTCTCGC
    TGCAACTCAG AGGAAAGGAG TCAGTCTCAG GAGAGACGTC ATTCTCCATC GCCCAGTGAG
    GGGAGGAAGC ACACAGCTCT CAGACAGGGA AATAATACAG GCAGCCCTAT TGCCTCCACG
    TCTGATTCAG GGACGCCTCG AGGACGCCGC CAACTCCCAC AGACACCCTC TCGCCCTCGG
    CCTCATATCT CCTACTCTCC TTTAGTGTGC CGTGCCCATC CAGCACCAGG GGATGATCGG
    CCACGAGCTC CACCCGCAGA GGAAGGAGAG GAGCCCACTG CAACCGGCCC AGGCACCGCC
    ATGGCCCACT CTACCCCTCA CCGCTACATC TCAGAGCCCT ACCTGCCACT GCAGGAGGAC
    GTCAACAGTC CAGACTCCAG TGGTGTGGAG GAGACTCTCA CTTTTGAGGC TGCAATAGCA
    ACCAGCCTGG GCCGCTCTAA TACCATTAGC TCGGCGCCAC AGCTACGTCA CTCCTGGCAG
    GTGCCTAATG GACATTTCCG CAAACGACTA GCACAAGCCA CAGCTGGGAC GTCCGGCTGT
    GAGCTTCTCA GTGACACCGA TGAGGATGAC CGCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212913.1
    CDS9..6587
    Translation

    Target ORF information:

    RefSeq Version XM_017357424.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357424.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGATCCTGG CCACCATTAT TGCTAACTGC ATCGTCCTAG CCTTGGAGCA GCACCTGCCT 
    GGAGAAGACA AGACACCCAT GGCTAAGAGA CTTGAAAAAA CAGAGCCCTA CTTCATAGGA
    ATGTTTTGCT TTGAAGCTGG AATAAAAATC ATTGCTTTGG GATTTGTATT CCACAAGGGC
    TCGTACCTGA GGAATGGTTG GAACGTCATG GACTTTATTG TCGTATCGAG TGGGATTCTG
    GCCACAGCAG GAGCCCACAT GAATATTTCA TTAGACCTGC GCACACTGAG GGCTGTGAGG
    GTATTACGGC CCCTCAAACT GGTCTCTGGC ATTCCTAGTC TGCAAATAGT TCTTAAATCC
    ATAATGAAGG CAATGGTGCC ATTGCTTCAG ATCGGACTGC TACTGTTTTT TGCTATTCTA
    ATGTTTGCCA TTATTGGATT AGAGTTCTAC AGTGGGAAAC TCCACAAGCA ATGTGAACCT
    GTAGGTAAAG ATTCTGAGAG AGCAGACTCG CCTGAGAATG AATTTCCATG TGGAAGCAAA
    CTGTGTCCTG ACCATTATAA CTGCTCTGGC TACTGGACGG GACCCAATGA TGGCATCACT
    CAGTTTGATA ACATCTTATT TTCTTTACTC ACCGTCTTCC AGTGCATCAC CATGGAAGGC
    TGGACGACCA TCCTCTATTA CACCAACGAT GCCTTAGGCT CTACTTGGAA TTGGCTCTAC
    TTCATTCCTC TAATCATAAT TGGCTCCTTC TTTGTGTTGA ATCTGGTATT AGGAGTCCTG
    TCAGGGGAGT TTGCTAAAGA GCGAGAGCGG GTAGAGAACC GGAGAGCCTT CATGAAGCTT
    AGGAGACAGC AGCAGATTGA GAGGGAGCTG AACGGATATC GGGCCTGGAT CGACCGTGCA
    GAGGAGGTTA TGCTTGCAGA GGAGAACAAG AATTCAGGAC CTTCTGCTTT GGATGTGCTA
    AAAAGAGCTA CCACCATAAA GAGAAGAGGA GTGGATGATG CTCGAAGAGC TCCAGAAGAG
    CAGTATGCTG ACATCTCATC TCTAGGGATG CCACTGAACA GAGCCAGCAT ACGCAGTGCT
    AAAAGAGGAC CAACATCCTA TTTCCGACGG AAAGAGCGCC TGCTACGGAT CTCCGTCCGC
    CGTATGGTGA AGACTCAGAC GTTCTACTGG ACTGTTCTGG GTCTGGTAGC CCTCAACACT
    CTCTGTGTAG CCATCGTACA TCACAAACAG CCACAGTGGT TATCTAGTCT CCTCTACTAT
    GCAGAGTTTT TGTTTCTGGG TTTGTTTCTT ACCGAGATGT TTCTGAAGAT GTATGGACTC
    GGACCCAGAC TCTATTTTCA CTCCTCCTTC AACTGCTTCG ATTGTGGGGT TATAGTAGGC
    AGCATCTTTG AGGTGTTCTG GGGCTTCTTC AGACCTGGCA CATCCTTTGG GATCAGCGTT
    CTTCGTGCCT TGCGGTTGTT GAGAATTTTC AAGATAACCA AATACTGGGC GTCCCTGAGG
    AACCTGGTCG TGTCTCTGAT GAACTCAATG AAGTCTATTA TCAGTTTGCT CTTCCTGCTT
    TTTCTCTTTA TTGTGGTCTT TGCACTTCTG GGCATGCAGC TCTTTGGTGG AAGGTTCATT
    TTTGATGAGT ACACTCCCAC CAATTTTGAC ACTTTCCCAG CAGCCATCAT GACAGTGTTT
    CAAATTCTGA CTGGTGAGGA CTGGAATGAA GTCATGTACA ATGGCATCCG GTCTCAAGGT
    GGCGTGAAAT CTGGGATGTG GTCGTCTGTC TACTTCATTG TTCTCACTCT GTTTGGAAAT
    TATACGCTGC TTAACGTCTT CTTGGCTATC GCTGTCGACA ATTTGGCCAA TGCCCAGGAG
    CTCACAAAGG ACGAAGAGGA GGAGGAAGAG GCGTTTAATC AGAAACATGC CCTGCAGAAA
    GCCAAGGAGG TTGGGCCCAT GTCCTCTTTC ATGTCCTCAG AGAGGAGGAG GAGGCCCTAC
    CTGTACAGGA AGCGTGCAAT TCATCGGAGC CGTGCAGCCT ACTCCTTCGA GGATGCAGAG
    AGTCTGGCTG ACGCTCGTCC ATTCAATGCC TCTAACTCTT TCATGTCCAG GAGAGAGAGG
    AGGAGGAGGT TGACCATGTC AGTGTGGGAG CAGAGGACCA GCCAGCTGCG GCGGCACAGG
    CAGATGTCCA GCCGCGAGAT CCTTTTCAGC AGCCCCAGTG AAGAACAGGA CGGACCACCA
    GGCTGCCCTC TCCACCGGAA ACCTGGACTA TCTCCATCTG GCAGCAAGCG CAGAGCCACT
    GAGACCACTT CAACCACAAT CATGCCAGAG GGTACCTTAG AGCCACCCAT GCCTATGGAC
    ATGCCGGTGG ATGAGCAAGC TCTGCCCATT TCTATTCCTG AACCCCCTTT AGCCATTCCA
    ATTCCAGAGC CACCAATGCC CGTGGACGCA TGCCTCCCTG AACCCCCAGT TACACTGAAT
    CTGCCCTTAA CAGAGCCATC TGAATCAAAG ACTTCACCCG AGGGTGCTCT GGACATCAAC
    AACCATTGCC CTAGACTAAA TGGGAACAGG CGGCAGCGGG CTGTCCGTAA GCACAGACCT
    CCTGCTATGG GGGACATGCT GACCCCTGAT ATGGGGCCCA GGCCCCGACG GCCACGCCAC
    CGCGAGCTTC ACACAGACCC CAGGGGAAAA GAGACTCTGC AAGGTGATGG TGTTGACGGT
    AAAGAACAAA AGCCAGGAAG TGAGGGTGAT GAAAAGAGTG ATGGAGAAAT CAGTCCAGAT
    GGCAGAGGGC TTGAACAAAG GCAGAACAAA CCAGACAACA CAGAATCATC CACAGAGTCC
    AAACCAGCAA ATTCCCCCCG CAAAATCCCA CCTCTTGACT CTGAACCTGC TACTCTACAG
    CTCACTGACA ACACACTGCA GCAGTCTTCA CAGGGAACTA CATGTGCCGG TGAGACCGAT
    AACAATAACC ACGGGTCCTT GAATCAGGAT AAGTCCTCCC TCAACACCAG CATCGTGATA
    GAGGTCACCG ACACTCAGCC ATCCATGGAG CTGACCTCCA TTACGGGTAA TAGTAAAGAT
    GTGGAGGCCT CAAAGCCAGA GAAAGAAGAA GAAACCCCAG ATGCACCCAA AGGGCCTCCA
    CCTGTCAGCA TGTTCATCTT CAAACCCAAC AACCCGGTCC GCAGGGCCTG CCACTACGTT
    GTCAACCTTC GTTATTTTGA GATGTCAATC CTTCTTGTGA TCGCCGCCAG CAGTATCGCA
    TTGGCTGCCG AAGACCCCGT CAACACTAAT TCGGACAGGA ACAAAGTTCT GCGCTATTTT
    GACTATGTAT TCACTGGGGT CTTCACGTTT GAAATGATAA TCAAGATGAT TGATCAGGGG
    TTGGTCTTAC ACTATGGCTC CTATTTTCGT GACCCGTGGA ACATTCTGGA TTTCATAGTT
    GTGGTGGGAG CTCTGGTGGC TTTTGCTCTC ACGAATGTGA TGGGGAGTAA CACAGGAAGA
    GACATTAAAA CCATTAAATC GCTGCGTGTA CTCCGAGTTC TGAGGCCTCT CAAGACGATT
    AAAAGACTTC CTAAACTCAA GGCTGTGTTT GACTGTGTGG TAACATCACT GAAGAATGTT
    TTCAACATCC TCATCGTCTA CAAGCTGTTT ATGTTCATAT TTGCCGTCAT TGCCGTGCAG
    CTTTTCAAAG GGAAATTTTA CTACTGTACC GACAGTTCCA AGGACACTGA AAACGAGTGC
    AAAGGCTACT ACATTGACTA TGATAAAGAC AAGAAAAAGG AGAAACGAGA GTGGAAGAGA
    CGCGATTTCC ATTATGATAA CGTTGTCTGG GCTCTGTTGA CTCTCTTCAC CGTATCCACT
    GGCGAAGGCT GGCCGCAAGT TCTGCAACAC TCTGTGGATG TGACAGAGGA GGACCGGGGT
    CCCAGTCAGG GGAACAGGAT GGAGATGTCT ATCTTCTATG TCATTTATTT TGTGGTGTTC
    CCCTTCTTCT TCGTCAACAT ATTTGTGGCC CTCATCATCA TCACTTTCCA AGAACAGGGT
    GACAAAATGA TGGAGGAGTG TAGTCTGGAG AAAAACGAGA GGGCTTGTAT CGACTTTGCC
    ATCAGTGCCA AACCTTTGAC TCGCTACATG CCCCAGAACA GACAGACCTT ACAGTACAGA
    CTGTGGCAAT TTGTGGTGTC GCCCTCATTT GAGTACACAG TACTGACAAT GATCGCCCTT
    AACACCATCG TTCTCATGAT GAAGCATCAT GATCCTCGTC TTGAAGGCTA TGACGATGTG
    CTCAAATATC TCAACACAAT TTTCACCGTT ATTTTCTCTG TTGAGTGTGT TCTCAAGATC
    CTGGCTTTTG GTATAATGAA CTATTTCAGA GACACATGGA ATATATTTGA CTTCATCACG
    GTTCTAGGAA GCATCACAGA AATTCTGGTG GACAAATTTC AGACTGGAAA AACTTTTAAT
    ATGGGCTTCC TGAAGCTATT CCGGGCAGCT CGGCTGATTA AACTCCTGCG TCAGGGCTAC
    ACCATCCGTA TTCTGCTCTG GACTTTTGTT CAGTCCTTCA AGGCTCTACC ATATGTCTGT
    CTGCTCATCG CCATGCTGTT TTTCATCTAT GCCATCATTG GCATGCAGGT ATTTGGCAAC
    ATCAAGATAA ATGAGAAAAC CCAAATCAAT GAACACAATA ACTTCAGGAC TTTCTTTGGT
    GCACTCATGC TTTTGTTCAG GAGTGCAACA GGTGAATCAT GGCAGGAAAT CATGCTATCG
    TGTTTAGGAG AGAAGGAGTG TGAAATTGAC ACTTCTGTCA ACTCAACAAC AAAGACAATA
    AAAAACGACT GCGGTACCGA CTTTGCCTAC TTCTACTTTG TCTCCTTCAT CTTCTTCAGT
    TCTTTTCTGA TGCTGAATCT GTTTGTGGCT GTGATCATGG ATAATTTTGA GTACCTGACA
    CGTGACTCAT CCATTCTGGG TCCTCACCAC TTAGATGAGT TTGTACGGAT CTGGGGAGAG
    TATGACCGTG CTGCATGTGG CCGTATTCAT TACACTGATA TGTACGAGAT GCTGACCTGC
    ATGTCGCCAC CGCTTGGCCT GGGCAAGAAA TGTCCCTCCA AAGTGGCGTA TAAGCGGTTG
    GTTCTGATGA ACATGCCAGT GGATGAAGAC ATGTCTGTCC ACTTCACCTC CACACTTATG
    GCCCTCATAC GCACCGCACT GGACATTAAA ATTGCTCGAG GAGGGGAAGA CAGGGGTCAG
    ATGGATGTTG AACTGCAGAA GGAAATCAGT GTCATCTGGC CGCATCTCTC ACAGAAATCT
    CTTGATCTGC TTGTACCCAT TCACAAAGCT AGTGACATGA CCATTGGGAA GATCTATGCA
    GCCATGATGA TCATGGACTA CTACAAGCAG AACAAAGCCA AGAAGCTCCG TCAGCAGCTC
    GAGGAACAGA AACACGCACC AATGTTCCAA CGCATGGATG CATCCTCGCT GCCGCAGGAC
    ATCCTCTGCA GTGCCAAGGC TTTGCCATTC CTGACTCACA GTGCAGGATC TGCCCTCACT
    AGAGGTGGGT TTGTGGCACT GAGTCCTATC TCTCCTCAAG ATCTCTTCAT GCAGCCCATG
    ACCCAAGACA GCATGGAGGA GAGGGAGGAA TACCAACACC CGTATCACCT GCAAAGCATG
    GTGCCTGAGC ACAAAGAATT TAGCAGTGTG ATGTGCCCCA CTAGCATGCA GGCTGATCCA
    CTGCAAGTGC CAGGAAGAGG CGTCTCTATC CGAGCCTCTT CCATGCCTCG CCTGGCGGTA
    GAGCCACAGG CCTCTTTAGA GAACAAACTG ATGAAGCGCT CATTCTCCAC CATCGGGGAT
    CAGTGTGTAA ATGGTGACTG GCAGGAAGAG TTCTCTCTGG AGAGGGTGAG TCCTGACCGG
    CTGGAGAGAC CCAGGCAAAG AAGCTTCAGA GGTGTCAGGT CAAACCTGCG GGAGCCAGTG
    GACAAAGAGA GAGGACGGTC TAAAGAACGA AGACACCTGC TGTCTCCAGA TGTGTCTCGC
    TGCAACTCAG AGGAAAGGAG TCAGTCTCAG GAGAGACGTC ATTCTCCATC GCCCAGTGAG
    GGGAGGAAGC ACACAGCTCT CAGACAGGGA AATAATACAG GCAGCCCTAT TGCCTCCACG
    TCTGATTCAG GGACGCCTCG AGGACGCCGC CAACTCCCAC AGACACCCTC TCGCCCTCGG
    CCTCATATCT CCTACTCTCC TTTAGTGTGC CGTGCCCATC CAGCACCAGG GGATGATCGG
    CCACGAGCTC CACCCGCAGA GGAAGGAGAG GAGCCCACTG CAACCGGCCC AGGCACCGCC
    ATGGCCCACT CTACCCCTCA CCGCTACATC TCAGAGCCCT ACCTGCCACT GCAGGAGGAC
    GTCAACAGTC CAGACTCCAG TGGTGTGGAG GAGACTCTCA CTTTTGAGGC TGCAATAGCA
    ACCAGCCTGG GCCGCTCTAA TACCATTAGC TCGGCGCCAC AGCTACGTCA CTCCTGGCAG
    GTGCCTAATG GACATTTCCG CAAACGACTA GCACAAGCCA CAGCTGGGAC GTCCGGCTGT
    GAGCTTCTCA GTGACACCGA TGAGGATGAC CGCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60029
    Clone ID Related Accession (Same CDS sequence) XM_017357425.1
    Accession Version XM_017357425.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGATCCTGG CCACCATTAT TGCTAACTGC ATCGTCCTAG CCTTGGAGCA GCACCTGCCT 
    GGAGAAGACA AGACACCCAT GGCTAAGAGA CTTGAAAAAA CAGAGCCCTA CTTCATAGGA
    ATGTTTTGCT TTGAAGCTGG AATAAAAATC ATTGCTTTGG GATTTGTATT CCACAAGGGC
    TCGTACCTGA GGAATGGTTG GAACGTCATG GACTTTATTG TCGTATCGAG TGGGATTCTG
    GCCACAGCAG GAGCCCACAT GAATATTTCA TTAGACCTGC GCACACTGAG GGCTGTGAGG
    GTATTACGGC CCCTCAAACT GGTCTCTGGC ATTCCTAGTC TGCAAATAGT TCTTAAATCC
    ATAATGAAGG CAATGGTGCC ATTGCTTCAG ATCGGACTGC TACTGTTTTT TGCTATTCTA
    ATGTTTGCCA TTATTGGATT AGAGTTCTAC AGTGGGAAAC TCCACAAGCA ATGTGAACCT
    GTAGGTAAAG ATTCTGAGAG AGCAGACTCG CCTGAGAATG AATTTCCATG TGGAAGCAAA
    CTGTGTCCTG ACCATTATAA CTGCTCTGGC TACTGGACGG GACCCAATGA TGGCATCACT
    CAGTTTGATA ACATCTTATT TTCTTTACTC ACCGTCTTCC AGTGCATCAC CATGGAAGGC
    TGGACGACCA TCCTCTATTA CACCAACGAT GCCTTAGGCT CTACTTGGAA TTGGCTCTAC
    TTCATTCCTC TAATCATAAT TGGCTCCTTC TTTGTGTTGA ATCTGGTATT AGGAGTCCTG
    TCAGGGGAGT TTGCTAAAGA GCGAGAGCGG GTAGAGAACC GGAGAGCCTT CATGAAGCTT
    AGGAGACAGC AGCAGATTGA GAGGGAGCTG AACGGATATC GGGCCTGGAT CGACCGTGCA
    GAGGAGGTTA TGCTTGCAGA GGAGAACAAG AATTCAGGAC CTTCTGCTTT GGATGTGCTA
    AAAAGAGCTA CCACCATAAA GAGAAGAGGA GTGGATGATG CTCGAAGAGC TCCAGAAGAG
    CAGTATGCTG ACATCTCATC TCTAGGGATG CCACTGAACA GAGCCAGCAT ACGCAGTGCT
    AAAAGAGGAC CAACATCCTA TTTCCGACGG AAAGAGCGCC TGCTACGGAT CTCCGTCCGC
    CGTATGGTGA AGACTCAGAC GTTCTACTGG ACTGTTCTGG GTCTGGTAGC CCTCAACACT
    CTCTGTGTAG CCATCGTACA TCACAAACAG CCACAGTGGT TATCTAGTCT CCTCTACTAT
    GCAGAGTTTT TGTTTCTGGG TTTGTTTCTT ACCGAGATGT TTCTGAAGAT GTATGGACTC
    GGACCCAGAC TCTATTTTCA CTCCTCCTTC AACTGCTTCG ATTGTGGGGT TATAGTAGGC
    AGCATCTTTG AGGTGTTCTG GGGCTTCTTC AGACCTGGCA CATCCTTTGG GATCAGCGTT
    CTTCGTGCCT TGCGGTTGTT GAGAATTTTC AAGATAACCA AATACTGGGC GTCCCTGAGG
    AACCTGGTCG TGTCTCTGAT GAACTCAATG AAGTCTATTA TCAGTTTGCT CTTCCTGCTT
    TTTCTCTTTA TTGTGGTCTT TGCACTTCTG GGCATGCAGC TCTTTGGTGG AAGGTTCATT
    TTTGATGAGT ACACTCCCAC CAATTTTGAC ACTTTCCCAG CAGCCATCAT GACAGTGTTT
    CAAATTCTGA CTGGTGAGGA CTGGAATGAA GTCATGTACA ATGGCATCCG GTCTCAAGGT
    GGCGTGAAAT CTGGGATGTG GTCGTCTGTC TACTTCATTG TTCTCACTCT GTTTGGAAAT
    TATACGCTGC TTAACGTCTT CTTGGCTATC GCTGTCGACA ATTTGGCCAA TGCCCAGGAG
    CTCACAAAGG ACGAAGAGGA GGAGGAAGAG GCGTTTAATC AGAAACATGC CCTGCAGAAA
    GCCAAGGAGG TTGGGCCCAT GTCCTCTTTC ATGTCCTCAG AGAGGAGGAG GAGGCCCTAC
    CTGTACAGGA AGCGTGCAAT TCATCGGAGC CGTGCAGCCT ACTCCTTCGA GGATGCAGAG
    AGTCTGGCTG ACGCTCGTCC ATTCAATGCC TCTAACTCTT TCATGTCCAG GAGAGAGAGG
    AGGAGGAGGT TGACCATGTC AGTGTGGGAG CAGAGGACCA GCCAGCTGCG GCGGCACAGG
    CAGATGTCCA GCCGCGAGAT CCTTTTCAGC AGCCCCAGTG AAGAACAGGA CGGACCACCA
    GGCTGCCCTC TCCACCGGAA ACCTGGACTA TCTCCATCTG GCAGCAAGCG CAGAGCCACT
    GAGACCACTT CAACCACAAT CATGCCAGAG GGTACCTTAG AGCCACCCAT GCCTATGGAC
    ATGCCGGTGG ATGAGCAAGC TCTGCCCATT TCTATTCCTG AACCCCCTTT AGCCATTCCA
    ATTCCAGAGC CACCAATGCC CGTGGACGCA TGCCTCCCTG AACCCCCAGT TACACTGAAT
    CTGCCCTTAA CAGAGCCATC TGAATCAAAG ACTTCACCCG AGGGTGCTCT GGACATCAAC
    AACCATTGCC CTAGACTAAA TGGGAACAGG CGGCAGCGGG CTGTCCGTAA GCACAGACCT
    CCTGCTATGG GGGACATGCT GACCCCTGAT ATGGGGCCCA GGCCCCGACG GCCACGCCAC
    CGCGAGCTTC ACACAGACCC CAGGGGAAAA GAGACTCTGC AAGGTGATGG TGTTGACGGT
    AAAGAACAAA AGCCAGGAAG TGAGGGTGAT GAAAAGAGTG ATGGAGAAAT CAGTCCAGAT
    GGCAGAGGGC TTGAACAAAG GCAGAACAAA CCAGACAACA CAGAATCATC CACAGAGTCC
    AAACCAGCAA ATTCCCCCCG CAAAATCCCA CCTCTTGACT CTGAACCTGC TACTCTACAG
    CTCACTGACA ACACACTGCA GCAGTCTTCA CAGGGAACTA CATGTGCCGG TGAGACCGAT
    AACAATAACC ACGGGTCCTT GAATCAGGAT AAGTCCTCCC TCAACACCAG CATCGTGATA
    GAGGTCACCG ACACTCAGCC ATCCATGGAG CTGACCTCCA TTACGGGTAA TAGTAAAGAT
    GTGGAGGCCT CAAAGCCAGA GAAAGAAGAA GAAACCCCAG ATGCACCCAA AGGGCCTCCA
    CCTGTCAGCA TGTTCATCTT CAAACCCAAC AACCCGGTCC GCAGGGCCTG CCACTACGTT
    GTCAACCTTC GTTATTTTGA GATGTCAATC CTTCTTGTGA TCGCCGCCAG CAGTATCGCA
    TTGGCTGCCG AAGACCCCGT CAACACTAAT TCGGACAGGA ACAAAGTTCT GCGCTATTTT
    GACTATGTAT TCACTGGGGT CTTCACGTTT GAAATGATAA TCAAGATGAT TGATCAGGGG
    TTGGTCTTAC ACTATGGCTC CTATTTTCGT GACCCGTGGA ACATTCTGGA TTTCATAGTT
    GTGGTGGGAG CTCTGGTGGC TTTTGCTCTC ACGAATGTGA TGGGGAGTAA CACAGGAAGA
    GACATTAAAA CCATTAAATC GCTGCGTGTA CTCCGAGTTC TGAGGCCTCT CAAGACGATT
    AAAAGACTTC CTAAACTCAA GGCTGTGTTT GACTGTGTGG TAACATCACT GAAGAATGTT
    TTCAACATCC TCATCGTCTA CAAGCTGTTT ATGTTCATAT TTGCCGTCAT TGCCGTGCAG
    CTTTTCAAAG GGAAATTTTA CTACTGTACC GACAGTTCCA AGGACACTGA AAACGAGTGC
    AAAGGCTACT ACATTGACTA TGATAAAGAC AAGAAAAAGG AGAAACGAGA GTGGAAGAGA
    CGCGATTTCC ATTATGATAA CGTTGTCTGG GCTCTGTTGA CTCTCTTCAC CGTATCCACT
    GGCGAAGGCT GGCCGCAAGT TCTGCAACAC TCTGTGGATG TGACAGAGGA GGACCGGGGT
    CCCAGTCAGG GGAACAGGAT GGAGATGTCT ATCTTCTATG TCATTTATTT TGTGGTGTTC
    CCCTTCTTCT TCGTCAACAT ATTTGTGGCC CTCATCATCA TCACTTTCCA AGAACAGGGT
    GACAAAATGA TGGAGGAGTG TAGTCTGGAG AAAAACGAGA GGGCTTGTAT CGACTTTGCC
    ATCAGTGCCA AACCTTTGAC TCGCTACATG CCCCAGAACA GACAGACCTT ACAGTACAGA
    CTGTGGCAAT TTGTGGTGTC GCCCTCATTT GAGTACACAG TACTGACAAT GATCGCCCTT
    AACACCATCG TTCTCATGAT GAAGCATCAT GATCCTCGTC TTGAAGGCTA TGACGATGTG
    CTCAAATATC TCAACACAAT TTTCACCGTT ATTTTCTCTG TTGAGTGTGT TCTCAAGATC
    CTGGCTTTTG GTATAATGAA CTATTTCAGA GACACATGGA ATATATTTGA CTTCATCACG
    GTTCTAGGAA GCATCACAGA AATTCTGGTG GACAAATTTC AGACTGGAAA AACTTTTAAT
    ATGGGCTTCC TGAAGCTATT CCGGGCAGCT CGGCTGATTA AACTCCTGCG TCAGGGCTAC
    ACCATCCGTA TTCTGCTCTG GACTTTTGTT CAGTCCTTCA AGGCTCTACC ATATGTCTGT
    CTGCTCATCG CCATGCTGTT TTTCATCTAT GCCATCATTG GCATGCAGGT ATTTGGCAAC
    ATCAAGATAA ATGAGAAAAC CCAAATCAAT GAACACAATA ACTTCAGGAC TTTCTTTGGT
    GCACTCATGC TTTTGTTCAG GAGTGCAACA GGTGAATCAT GGCAGGAAAT CATGCTATCG
    TGTTTAGGAG AGAAGGAGTG TGAAATTGAC ACTTCTGTCA ACTCAACAAC AAAGACAATA
    AAAAACGACT GCGGTACCGA CTTTGCCTAC TTCTACTTTG TCTCCTTCAT CTTCTTCAGT
    TCTTTTCTGA TGCTGAATCT GTTTGTGGCT GTGATCATGG ATAATTTTGA GTACCTGACA
    CGTGACTCAT CCATTCTGGG TCCTCACCAC TTAGATGAGT TTGTACGGAT CTGGGGAGAG
    TATGACCGTG CTGCATGTGG AAAACTACAC TATAAGGAGA TGTACAAAGT AGTACGCACA
    ATATCACCTC CTCTGGGCTT TGGAAAGAAT TGCCCCTACA GGGTGGCATG CAAGCGGTTG
    GTTCTGATGA ACATGCCAGT GGATGAAGAC ATGTCTGTCC ACTTCACCTC CACACTTATG
    GCCCTCATAC GCACCGCACT GGACATTAAA ATTGCTCGAG GAGGGGAAGA CAGGGGTCAG
    ATGGATGTTG AACTGCAGAA GGAAATCAGT GTCATCTGGC CGCATCTCTC ACAGAAATCT
    CTTGATCTGC TTGTACCCAT TCACAAAGCT AGTGACATGA CCATTGGGAA GATCTATGCA
    GCCATGATGA TCATGGACTA CTACAAGCAG AACAAAGCCA AGAAGCTCCG TCAGCAGCTC
    GAGGAACAGA AACACGCACC AATGTTCCAA CGCATGGATG CATCCTCGCT GCCGCAGGAC
    ATCCTCTGCA GTGCCAAGGC TTTGCCATTC CTGACTCACA GTGCAGGATC TGCCCTCACT
    AGAGGTGGGT TTGTGGCACT GAGTCCTATC TCTCCTCAAG ATCTCTTCAT GCAGCCCATG
    ACCCAAGACA GCATGGAGGA GAGGGAGGAA TACCAACACC CGTATCACCT GCAAAGCATG
    GTGCCTGAGC ACAAAGAATT TAGCAGTGTG ATGTGCCCCA CTAGCATGCA GGCTGATCCA
    CTGCAAGTGC CAGGAAGAGG CGTCTCTATC CGAGCCTCTT CCATGCCTCG CCTGGCGGTA
    GAGCCACAGG CCTCTTTAGA GAACAAACTG ATGAAGCGCT CATTCTCCAC CATCGGGGAT
    CAGTGTGTAA ATGGTGACTG GCAGGAAGAG TTCTCTCTGG AGAGGGTGAG TCCTGACCGG
    CTGGAGAGAC CCAGGCAAAG AAGCTTCAGA GGTGTCAGGT CAAACCTGCG GGAGCCAGTG
    GACAAAGAGA GAGGACGGTC TAAAGAACGA AGACACCTGC TGTCTCCAGA TGTGTCTCGC
    TGCAACTCAG AGGAAAGGAG TCAGTCTCAG GAGAGACGTC ATTCTCCATC GCCCAGTGAG
    GGGAGGAAGC ACACAGCTCT CAGACAGGGA AATAATACAG GCAGCCCTAT TGCCTCCACG
    TCTGATTCAG GGACGCCTCG AGGACGCCGC CAACTCCCAC AGACACCCTC TCGCCCTCGG
    CCTCATATCT CCTACTCTCC TTTAGTGTGC CGTGCCCATC CAGCACCAGG GGATGATCGG
    CCACGAGCTC CACCCGCAGA GGAAGGAGAG GAGCCCACTG CAACCGGCCC AGGCACCGCC
    ATGGCCCACT CTACCCCTCA CCGCTACATC TCAGAGCCCT ACCTGCCACT GCAGGAGGAC
    GTCAACAGTC CAGACTCCAG TGGTGTGGAG GAGACTCTCA CTTTTGAGGC TGCAATAGCA
    ACCAGCCTGG GCCGCTCTAA TACCATTAGC TCGGCGCCAC AGCTACGTCA CTCCTGGCAG
    GTGCCTAATG GACATTTCCG CAAACGACTA GCACAAGCCA CAGCTGGGAC GTCCGGCTGT
    GAGCTTCTCA GTGACACCGA TGAGGATGAC CGCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212914.1
    CDS9..6587
    Translation

    Target ORF information:

    RefSeq Version XM_017357425.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357425.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGATCCTGG CCACCATTAT TGCTAACTGC ATCGTCCTAG CCTTGGAGCA GCACCTGCCT 
    GGAGAAGACA AGACACCCAT GGCTAAGAGA CTTGAAAAAA CAGAGCCCTA CTTCATAGGA
    ATGTTTTGCT TTGAAGCTGG AATAAAAATC ATTGCTTTGG GATTTGTATT CCACAAGGGC
    TCGTACCTGA GGAATGGTTG GAACGTCATG GACTTTATTG TCGTATCGAG TGGGATTCTG
    GCCACAGCAG GAGCCCACAT GAATATTTCA TTAGACCTGC GCACACTGAG GGCTGTGAGG
    GTATTACGGC CCCTCAAACT GGTCTCTGGC ATTCCTAGTC TGCAAATAGT TCTTAAATCC
    ATAATGAAGG CAATGGTGCC ATTGCTTCAG ATCGGACTGC TACTGTTTTT TGCTATTCTA
    ATGTTTGCCA TTATTGGATT AGAGTTCTAC AGTGGGAAAC TCCACAAGCA ATGTGAACCT
    GTAGGTAAAG ATTCTGAGAG AGCAGACTCG CCTGAGAATG AATTTCCATG TGGAAGCAAA
    CTGTGTCCTG ACCATTATAA CTGCTCTGGC TACTGGACGG GACCCAATGA TGGCATCACT
    CAGTTTGATA ACATCTTATT TTCTTTACTC ACCGTCTTCC AGTGCATCAC CATGGAAGGC
    TGGACGACCA TCCTCTATTA CACCAACGAT GCCTTAGGCT CTACTTGGAA TTGGCTCTAC
    TTCATTCCTC TAATCATAAT TGGCTCCTTC TTTGTGTTGA ATCTGGTATT AGGAGTCCTG
    TCAGGGGAGT TTGCTAAAGA GCGAGAGCGG GTAGAGAACC GGAGAGCCTT CATGAAGCTT
    AGGAGACAGC AGCAGATTGA GAGGGAGCTG AACGGATATC GGGCCTGGAT CGACCGTGCA
    GAGGAGGTTA TGCTTGCAGA GGAGAACAAG AATTCAGGAC CTTCTGCTTT GGATGTGCTA
    AAAAGAGCTA CCACCATAAA GAGAAGAGGA GTGGATGATG CTCGAAGAGC TCCAGAAGAG
    CAGTATGCTG ACATCTCATC TCTAGGGATG CCACTGAACA GAGCCAGCAT ACGCAGTGCT
    AAAAGAGGAC CAACATCCTA TTTCCGACGG AAAGAGCGCC TGCTACGGAT CTCCGTCCGC
    CGTATGGTGA AGACTCAGAC GTTCTACTGG ACTGTTCTGG GTCTGGTAGC CCTCAACACT
    CTCTGTGTAG CCATCGTACA TCACAAACAG CCACAGTGGT TATCTAGTCT CCTCTACTAT
    GCAGAGTTTT TGTTTCTGGG TTTGTTTCTT ACCGAGATGT TTCTGAAGAT GTATGGACTC
    GGACCCAGAC TCTATTTTCA CTCCTCCTTC AACTGCTTCG ATTGTGGGGT TATAGTAGGC
    AGCATCTTTG AGGTGTTCTG GGGCTTCTTC AGACCTGGCA CATCCTTTGG GATCAGCGTT
    CTTCGTGCCT TGCGGTTGTT GAGAATTTTC AAGATAACCA AATACTGGGC GTCCCTGAGG
    AACCTGGTCG TGTCTCTGAT GAACTCAATG AAGTCTATTA TCAGTTTGCT CTTCCTGCTT
    TTTCTCTTTA TTGTGGTCTT TGCACTTCTG GGCATGCAGC TCTTTGGTGG AAGGTTCATT
    TTTGATGAGT ACACTCCCAC CAATTTTGAC ACTTTCCCAG CAGCCATCAT GACAGTGTTT
    CAAATTCTGA CTGGTGAGGA CTGGAATGAA GTCATGTACA ATGGCATCCG GTCTCAAGGT
    GGCGTGAAAT CTGGGATGTG GTCGTCTGTC TACTTCATTG TTCTCACTCT GTTTGGAAAT
    TATACGCTGC TTAACGTCTT CTTGGCTATC GCTGTCGACA ATTTGGCCAA TGCCCAGGAG
    CTCACAAAGG ACGAAGAGGA GGAGGAAGAG GCGTTTAATC AGAAACATGC CCTGCAGAAA
    GCCAAGGAGG TTGGGCCCAT GTCCTCTTTC ATGTCCTCAG AGAGGAGGAG GAGGCCCTAC
    CTGTACAGGA AGCGTGCAAT TCATCGGAGC CGTGCAGCCT ACTCCTTCGA GGATGCAGAG
    AGTCTGGCTG ACGCTCGTCC ATTCAATGCC TCTAACTCTT TCATGTCCAG GAGAGAGAGG
    AGGAGGAGGT TGACCATGTC AGTGTGGGAG CAGAGGACCA GCCAGCTGCG GCGGCACAGG
    CAGATGTCCA GCCGCGAGAT CCTTTTCAGC AGCCCCAGTG AAGAACAGGA CGGACCACCA
    GGCTGCCCTC TCCACCGGAA ACCTGGACTA TCTCCATCTG GCAGCAAGCG CAGAGCCACT
    GAGACCACTT CAACCACAAT CATGCCAGAG GGTACCTTAG AGCCACCCAT GCCTATGGAC
    ATGCCGGTGG ATGAGCAAGC TCTGCCCATT TCTATTCCTG AACCCCCTTT AGCCATTCCA
    ATTCCAGAGC CACCAATGCC CGTGGACGCA TGCCTCCCTG AACCCCCAGT TACACTGAAT
    CTGCCCTTAA CAGAGCCATC TGAATCAAAG ACTTCACCCG AGGGTGCTCT GGACATCAAC
    AACCATTGCC CTAGACTAAA TGGGAACAGG CGGCAGCGGG CTGTCCGTAA GCACAGACCT
    CCTGCTATGG GGGACATGCT GACCCCTGAT ATGGGGCCCA GGCCCCGACG GCCACGCCAC
    CGCGAGCTTC ACACAGACCC CAGGGGAAAA GAGACTCTGC AAGGTGATGG TGTTGACGGT
    AAAGAACAAA AGCCAGGAAG TGAGGGTGAT GAAAAGAGTG ATGGAGAAAT CAGTCCAGAT
    GGCAGAGGGC TTGAACAAAG GCAGAACAAA CCAGACAACA CAGAATCATC CACAGAGTCC
    AAACCAGCAA ATTCCCCCCG CAAAATCCCA CCTCTTGACT CTGAACCTGC TACTCTACAG
    CTCACTGACA ACACACTGCA GCAGTCTTCA CAGGGAACTA CATGTGCCGG TGAGACCGAT
    AACAATAACC ACGGGTCCTT GAATCAGGAT AAGTCCTCCC TCAACACCAG CATCGTGATA
    GAGGTCACCG ACACTCAGCC ATCCATGGAG CTGACCTCCA TTACGGGTAA TAGTAAAGAT
    GTGGAGGCCT CAAAGCCAGA GAAAGAAGAA GAAACCCCAG ATGCACCCAA AGGGCCTCCA
    CCTGTCAGCA TGTTCATCTT CAAACCCAAC AACCCGGTCC GCAGGGCCTG CCACTACGTT
    GTCAACCTTC GTTATTTTGA GATGTCAATC CTTCTTGTGA TCGCCGCCAG CAGTATCGCA
    TTGGCTGCCG AAGACCCCGT CAACACTAAT TCGGACAGGA ACAAAGTTCT GCGCTATTTT
    GACTATGTAT TCACTGGGGT CTTCACGTTT GAAATGATAA TCAAGATGAT TGATCAGGGG
    TTGGTCTTAC ACTATGGCTC CTATTTTCGT GACCCGTGGA ACATTCTGGA TTTCATAGTT
    GTGGTGGGAG CTCTGGTGGC TTTTGCTCTC ACGAATGTGA TGGGGAGTAA CACAGGAAGA
    GACATTAAAA CCATTAAATC GCTGCGTGTA CTCCGAGTTC TGAGGCCTCT CAAGACGATT
    AAAAGACTTC CTAAACTCAA GGCTGTGTTT GACTGTGTGG TAACATCACT GAAGAATGTT
    TTCAACATCC TCATCGTCTA CAAGCTGTTT ATGTTCATAT TTGCCGTCAT TGCCGTGCAG
    CTTTTCAAAG GGAAATTTTA CTACTGTACC GACAGTTCCA AGGACACTGA AAACGAGTGC
    AAAGGCTACT ACATTGACTA TGATAAAGAC AAGAAAAAGG AGAAACGAGA GTGGAAGAGA
    CGCGATTTCC ATTATGATAA CGTTGTCTGG GCTCTGTTGA CTCTCTTCAC CGTATCCACT
    GGCGAAGGCT GGCCGCAAGT TCTGCAACAC TCTGTGGATG TGACAGAGGA GGACCGGGGT
    CCCAGTCAGG GGAACAGGAT GGAGATGTCT ATCTTCTATG TCATTTATTT TGTGGTGTTC
    CCCTTCTTCT TCGTCAACAT ATTTGTGGCC CTCATCATCA TCACTTTCCA AGAACAGGGT
    GACAAAATGA TGGAGGAGTG TAGTCTGGAG AAAAACGAGA GGGCTTGTAT CGACTTTGCC
    ATCAGTGCCA AACCTTTGAC TCGCTACATG CCCCAGAACA GACAGACCTT ACAGTACAGA
    CTGTGGCAAT TTGTGGTGTC GCCCTCATTT GAGTACACAG TACTGACAAT GATCGCCCTT
    AACACCATCG TTCTCATGAT GAAGCATCAT GATCCTCGTC TTGAAGGCTA TGACGATGTG
    CTCAAATATC TCAACACAAT TTTCACCGTT ATTTTCTCTG TTGAGTGTGT TCTCAAGATC
    CTGGCTTTTG GTATAATGAA CTATTTCAGA GACACATGGA ATATATTTGA CTTCATCACG
    GTTCTAGGAA GCATCACAGA AATTCTGGTG GACAAATTTC AGACTGGAAA AACTTTTAAT
    ATGGGCTTCC TGAAGCTATT CCGGGCAGCT CGGCTGATTA AACTCCTGCG TCAGGGCTAC
    ACCATCCGTA TTCTGCTCTG GACTTTTGTT CAGTCCTTCA AGGCTCTACC ATATGTCTGT
    CTGCTCATCG CCATGCTGTT TTTCATCTAT GCCATCATTG GCATGCAGGT ATTTGGCAAC
    ATCAAGATAA ATGAGAAAAC CCAAATCAAT GAACACAATA ACTTCAGGAC TTTCTTTGGT
    GCACTCATGC TTTTGTTCAG GAGTGCAACA GGTGAATCAT GGCAGGAAAT CATGCTATCG
    TGTTTAGGAG AGAAGGAGTG TGAAATTGAC ACTTCTGTCA ACTCAACAAC AAAGACAATA
    AAAAACGACT GCGGTACCGA CTTTGCCTAC TTCTACTTTG TCTCCTTCAT CTTCTTCAGT
    TCTTTTCTGA TGCTGAATCT GTTTGTGGCT GTGATCATGG ATAATTTTGA GTACCTGACA
    CGTGACTCAT CCATTCTGGG TCCTCACCAC TTAGATGAGT TTGTACGGAT CTGGGGAGAG
    TATGACCGTG CTGCATGTGG AAAACTACAC TATAAGGAGA TGTACAAAGT AGTACGCACA
    ATATCACCTC CTCTGGGCTT TGGAAAGAAT TGCCCCTACA GGGTGGCATG CAAGCGGTTG
    GTTCTGATGA ACATGCCAGT GGATGAAGAC ATGTCTGTCC ACTTCACCTC CACACTTATG
    GCCCTCATAC GCACCGCACT GGACATTAAA ATTGCTCGAG GAGGGGAAGA CAGGGGTCAG
    ATGGATGTTG AACTGCAGAA GGAAATCAGT GTCATCTGGC CGCATCTCTC ACAGAAATCT
    CTTGATCTGC TTGTACCCAT TCACAAAGCT AGTGACATGA CCATTGGGAA GATCTATGCA
    GCCATGATGA TCATGGACTA CTACAAGCAG AACAAAGCCA AGAAGCTCCG TCAGCAGCTC
    GAGGAACAGA AACACGCACC AATGTTCCAA CGCATGGATG CATCCTCGCT GCCGCAGGAC
    ATCCTCTGCA GTGCCAAGGC TTTGCCATTC CTGACTCACA GTGCAGGATC TGCCCTCACT
    AGAGGTGGGT TTGTGGCACT GAGTCCTATC TCTCCTCAAG ATCTCTTCAT GCAGCCCATG
    ACCCAAGACA GCATGGAGGA GAGGGAGGAA TACCAACACC CGTATCACCT GCAAAGCATG
    GTGCCTGAGC ACAAAGAATT TAGCAGTGTG ATGTGCCCCA CTAGCATGCA GGCTGATCCA
    CTGCAAGTGC CAGGAAGAGG CGTCTCTATC CGAGCCTCTT CCATGCCTCG CCTGGCGGTA
    GAGCCACAGG CCTCTTTAGA GAACAAACTG ATGAAGCGCT CATTCTCCAC CATCGGGGAT
    CAGTGTGTAA ATGGTGACTG GCAGGAAGAG TTCTCTCTGG AGAGGGTGAG TCCTGACCGG
    CTGGAGAGAC CCAGGCAAAG AAGCTTCAGA GGTGTCAGGT CAAACCTGCG GGAGCCAGTG
    GACAAAGAGA GAGGACGGTC TAAAGAACGA AGACACCTGC TGTCTCCAGA TGTGTCTCGC
    TGCAACTCAG AGGAAAGGAG TCAGTCTCAG GAGAGACGTC ATTCTCCATC GCCCAGTGAG
    GGGAGGAAGC ACACAGCTCT CAGACAGGGA AATAATACAG GCAGCCCTAT TGCCTCCACG
    TCTGATTCAG GGACGCCTCG AGGACGCCGC CAACTCCCAC AGACACCCTC TCGCCCTCGG
    CCTCATATCT CCTACTCTCC TTTAGTGTGC CGTGCCCATC CAGCACCAGG GGATGATCGG
    CCACGAGCTC CACCCGCAGA GGAAGGAGAG GAGCCCACTG CAACCGGCCC AGGCACCGCC
    ATGGCCCACT CTACCCCTCA CCGCTACATC TCAGAGCCCT ACCTGCCACT GCAGGAGGAC
    GTCAACAGTC CAGACTCCAG TGGTGTGGAG GAGACTCTCA CTTTTGAGGC TGCAATAGCA
    ACCAGCCTGG GCCGCTCTAA TACCATTAGC TCGGCGCCAC AGCTACGTCA CTCCTGGCAG
    GTGCCTAATG GACATTTCCG CAAACGACTA GCACAAGCCA CAGCTGGGAC GTCCGGCTGT
    GAGCTTCTCA GTGACACCGA TGAGGATGAC CGCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60030
    Clone ID Related Accession (Same CDS sequence) XM_017357426.1
    Accession Version XM_017357426.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    781
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    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGATCCTGG CCACCATTAT TGCTAACTGC ATCGTCCTAG CCTTGGAGCA GCACCTGCCT 
    GGAGAAGACA AGACACCCAT GGCTAAGAGA CTTGAAAAAA CAGAGCCCTA CTTCATAGGA
    ATGTTTTGCT TTGAAGCTGG AATAAAAATC ATTGCTTTGG GATTTGTATT CCACAAGGGC
    TCGTACCTGA GGAATGGTTG GAACGTCATG GACTTTATTG TCGTATCGAG TGGGATTCTG
    GCCACAGCAG GAGCCCACAT GAATATTTCA TTAGACCTGC GCACACTGAG GGCTGTGAGG
    GTATTACGGC CCCTCAAACT GGTCTCTGGC ATTCCTAGTC TGCAAATAGT TCTTAAATCC
    ATAATGAAGG CAATGGTGCC ATTGCTTCAG ATCGGACTGC TACTGTTTTT TGCTATTCTA
    ATGTTTGCCA TTATTGGATT AGAGTTCTAC AGTGGGAAAC TCCACAAGCA ATGTGAACCT
    GTAGGTAAAG ATTCTGAGAG AGCAGACTCG CCTGAGAATG AATTTCCATG TGGAAGCAAA
    CTGTGTCCTG ACCATTATAA CTGCTCTGGC TACTGGACGG GACCCAATGA TGGCATCACT
    CAGTTTGATA ACATCTTATT TTCTTTACTC ACCGTCTTCC AGTGCATCAC CATGGAAGGC
    TGGACGACCA TCCTCTATTA CACCAACGAT GCCTTAGGCT CTACTTGGAA TTGGCTCTAC
    TTCATTCCTC TAATCATAAT TGGCTCCTTC TTTGTGTTGA ATCTGGTATT AGGAGTCCTG
    TCAGGGGAGT TTGCTAAAGA GCGAGAGCGG GTAGAGAACC GGAGAGCCTT CATGAAGCTT
    AGGAGACAGC AGCAGATTGA GAGGGAGCTG AACGGATATC GGGCCTGGAT CGACCGTGCA
    GAGGAGGTTA TGCTTGCAGA GGAGAACAAG AATTCAGGAC CTTCTGCTTT GGATGTGCTA
    AAAAGAGCTA CCACCATAAA GAGAAGAGGA GTGGATGATG CTCGAAGAGC TCCAGAAGAG
    CAGTATGCTG ACATCTCATC TCTAGGGATG CCACTGAACA GAGCCAGCAT ACGCAGTGCT
    AAAAGAGGAC CAACATCCTA TTTCCGACGG AAAGAGCGCC TGCTACGGAT CTCCGTCCGC
    CGTATGGTGA AGACTCAGAC GTTCTACTGG ACTGTTCTGG GTCTGGTAGC CCTCAACACT
    CTCTGTGTAG CCATCGTACA TCACAAACAG CCACAGTGGT TATCTAGTCT CCTCTACTAT
    GCAGAGTTTT TGTTTCTGGG TTTGTTTCTT ACCGAGATGT TTCTGAAGAT GTATGGACTC
    GGACCCAGAC TCTATTTTCA CTCCTCCTTC AACTGCTTCG ATTGTGGGGT TATAGTAGGC
    AGCATCTTTG AGGTGTTCTG GGGCTTCTTC AGACCTGGCA CATCCTTTGG GATCAGCGTT
    CTTCGTGCCT TGCGGTTGTT GAGAATTTTC AAGATAACCA AATACTGGGC GTCCCTGAGG
    AACCTGGTCG TGTCTCTGAT GAACTCAATG AAGTCTATTA TCAGTTTGCT CTTCCTGCTT
    TTTCTCTTTA TTGTGGTCTT TGCACTTCTG GGCATGCAGC TCTTTGGTGG AAGGTTCATT
    TTTGATGAGT ACACTCCCAC CAATTTTGAC ACTTTCCCAG CAGCCATCAT GACAGTGTTT
    CAAATTCTGA CTGGTGAGGA CTGGAATGAA GTCATGTACA ATGGCATCCG GTCTCAAGGT
    GGCGTGAAAT CTGGGATGTG GTCGTCTGTC TACTTCATTG TTCTCACTCT GTTTGGAAAT
    TATACGCTGC TTAACGTCTT CTTGGCTATC GCTGTCGACA ATTTGGCCAA TGCCCAGGAG
    CTCACAAAGG ACGAAGAGGA GGAGGAAGAG GCGTTTAATC AGAAACATGC CCTGCAGAAA
    GCCAAGGAGG TTGGGCCCAT GTCCTCTTTC ATGTCCTCAG AGAGGAGGAG GAGGCCCTAC
    CTGTACAGGA AGCGTGCAAT TCATCGGAGC CGTGCAGCCT ACTCCTTCGA GGATGCAGAG
    AGTCTGGCTG ACGCTCGTCC ATTCAATGCC TCTAACTCTT TCATGTCCAG GAGAGAGAGG
    AGGAGGAGGT TGACCATGTC AGTGTGGGAG CAGAGGACCA GCCAGCTGCG GCGGCACAGG
    CAGATGTCCA GCCGCGAGAT CCTTTTCAGC AGCCCCAGTG AAGAACAGGA CGGACCACCA
    GGCTGCCCTC TCCACCGGAA ACCTGGACTA TCTCCATCTG GCAGCAAGCG CAGAGCCACT
    GAGACCACTT CAACCACAAT CATGCCAGAG GGTACCTTAG AGCCACCCAT GCCTATGGAC
    ATGCCGGTGG ATGAGCAAGC TCTGCCCATT TCTATTCCTG AACCCCCTTT AGCCATTCCA
    ATTCCAGAGC CACCAATGCC CGTGGACGCA TGCCTCCCTG AACCCCCAGT TACACTGAAT
    CTGCCCTTAA CAGAGCCATC TGAATCAAAG ACTTCACCCG AGGGTGCTCT GGACATCAAC
    AACCATTGCC CTAGACTAAA TGGGAACAGG CGGCAGCGGG CTGTCCGTAA GCACAGACCT
    CCTGCTATGG GGGACATGCT GACCCCTGAT ATGGGGCCCA GGCCCCGACG GCCACGCCAC
    CGCGAGCTTC ACACAGACCC CAGGGGAAAA GAGACTCTGC AAGGTGATGG TGTTGACGGT
    AAAGAACAAA AGCCAGGAAG TGAGGGTGAT GAAAAGAGTG ATGGAGAAAT CAGTCCAGAT
    GGCAGAGGGC TTGAACAAAG GCAGAACAAA CCAGACAACA CAGAATCATC CACAGAGTCC
    AAACCAGCAA ATTCCCCCCG CAAAATCCCA CCTCTTGACT CTGAACCTGC TACTCTACAG
    CTCACTGACA ACACACTGCA GCAGTCTTCA CAGGGAACTA CATGTGCCGG TGAGACCGAT
    AACAATAACC ACGGGTCCTT GAATCAGGAT AAGTCCTCCC TCAACACCAG CATCGTGATA
    GAGGTCACCG ACACTCAGCC ATCCATGGAG CTGACCTCCA TTACGGGTAA TAGTAAAGAT
    GTGGAGGCCT CAAAGCCAGA GAAAGAAGAA GAAACCCCAG ATGCACCCAA AGGGCCTCCA
    CCTGTCAGCA TGTTCATCTT CAAACCCAAC AACCCGGTCC GCAGGGCCTG CCACTACGTT
    GTCAACCTTC GTTATTTTGA GATGTCAATC CTTCTTGTGA TCGCCGCCAG CAGTATCGCA
    TTGGCTGCCG AAGACCCCGT CAACACTAAT TCGGACAGGA ACAAAGTTCT GCGCTATTTT
    GACTATGTAT TCACTGGGGT CTTCACGTTT GAAATGATAA TCAAGATGAT TGATCAGGGG
    TTGGTCTTAC ACTATGGCTC CTATTTTCGT GACCCGTGGA ACATTCTGGA TTTCATAGTT
    GTGGTGGGAG CTCTGGTGGC TTTTGCTCTC ACGAGTAACA CAGGAAGAGA CATTAAAACC
    ATTAAATCGC TGCGTGTACT CCGAGTTCTG AGGCCTCTCA AGACGATTAA AAGACTTCCT
    AAACTCAAGG CTGTGTTTGA CTGTGTGGTA ACATCACTGA AGAATGTTTT CAACATCCTC
    ATCGTCTACA AGCTGTTTAT GTTCATATTT GCCGTCATTG CCGTGCAGCT TTTCAAAGGG
    AAATTTTACT ACTGTACCGA CAGTTCCAAG GACACTGAAA ACGAGTGCAA AGGCTACTAC
    ATTGACTATG ATAAAGACAA GAAAAAGGAG AAACGAGAGT GGAAGAGACG CGATTTCCAT
    TATGATAACG TTGTCTGGGC TCTGTTGACT CTCTTCACCG TATCCACTGG CGAAGGCTGG
    CCGCAAGTTC TGCAACACTC TGTGGATGTG ACAGAGGAGG ACCGGGGTCC CAGTCAGGGG
    AACAGGATGG AGATGTCTAT CTTCTATGTC ATTTATTTTG TGGTGTTCCC CTTCTTCTTC
    GTCAACATAT TTGTGGCCCT CATCATCATC ACTTTCCAAG AACAGGGTGA CAAAATGATG
    GAGGAGTGTA GTCTGGAGAA AAACGAGAGG GCTTGTATCG ACTTTGCCAT CAGTGCCAAA
    CCTTTGACTC GCTACATGCC CCAGAACAGA CAGACCTTAC AGTACAGACT GTGGCAATTT
    GTGGTGTCGC CCTCATTTGA GTACACAGTA CTGACAATGA TCGCCCTTAA CACCATCGTT
    CTCATGATGA AGCATCATGA TCCTCGTCTT GAAGGCTATG ACGATGTGCT CAAATATCTC
    AACACAATTT TCACCGTTAT TTTCTCTGTT GAGTGTGTTC TCAAGATCCT GGCTTTTGGT
    ATAATGAACT ATTTCAGAGA CACATGGAAT ATATTTGACT TCATCACGGT TCTAGGAAGC
    ATCACAGAAA TTCTGGTGGA CAAATTTCAG ACTGGAAAAA CTTTTAATAT GGGCTTCCTG
    AAGCTATTCC GGGCAGCTCG GCTGATTAAA CTCCTGCGTC AGGGCTACAC CATCCGTATT
    CTGCTCTGGA CTTTTGTTCA GTCCTTCAAG GCTCTACCAT ATGTCTGTCT GCTCATCGCC
    ATGCTGTTTT TCATCTATGC CATCATTGGC ATGCAGGTAT TTGGCAACAT CAAGATAAAT
    GAGAAAACCC AAATCAATGA ACACAATAAC TTCAGGACTT TCTTTGGTGC ACTCATGCTT
    TTGTTCAGGA GTGCAACAGG TGAATCATGG CAGGAAATCA TGCTATCGTG TTTAGGAGAG
    AAGGAGTGTG AAATTGACAC TTCTGTCAAC TCAACAACAA AGACAATAAA AAACGACTGC
    GGTACCGACT TTGCCTACTT CTACTTTGTC TCCTTCATCT TCTTCAGTTC TTTTCTGATG
    CTGAATCTGT TTGTGGCTGT GATCATGGAT AATTTTGAGT ACCTGACACG TGACTCATCC
    ATTCTGGGTC CTCACCACTT AGATGAGTTT GTACGGATCT GGGGAGAGTA TGACCGTGCT
    GCATGTGGCC GTATTCATTA CACTGATATG TACGAGATGC TGACCTGCAT GTCGCCACCG
    CTTGGCCTGG GCAAGAAATG TCCCTCCAAA GTGGCGTATA AGCGGTTGGT TCTGATGAAC
    ATGCCAGTGG ATGAAGACAT GTCTGTCCAC TTCACCTCCA CACTTATGGC CCTCATACGC
    ACCGCACTGG ACATTAAAAT TGCTCGAGGA GGGGAAGACA GGGGTCAGAT GGATGTTGAA
    CTGCAGAAGG AAATCAGTGT CATCTGGCCG CATCTCTCAC AGAAATCTCT TGATCTGCTT
    GTACCCATTC ACAAAGCTAG TGACATGACC ATTGGGAAGA TCTATGCAGC CATGATGATC
    ATGGACTACT ACAAGCAGAA CAAAGCCAAG AAGCTCCGTC AGCAGCTCGA GGAACAGAAA
    CACGCACCAA TGTTCCAACG CATGGATGCA TCCTCGCTGC CGCAGGACAT CCTCTGCAGT
    GCCAAGGCTT TGCCATTCCT GACTCACAGT GCAGGATCTG CCCTCACTAG AGGTGGGTTT
    GTGGCACTGA GTCCTATCTC TCCTCAAGAT CTCTTCATGC AGCCCATGAC CCAAGACAGC
    ATGGAGGAGA GGGAGGAATA CCAACACCCG TATCACCTGC AAAGCATGGT GCCTGAGCAC
    AAAGAATTTA GCAGTGTGAT GTGCCCCACT AGCATGCAGG CTGATCCACT GCAAGTGCCA
    GGAAGAGGCG TCTCTATCCG AGCCTCTTCC ATGCCTCGCC TGGCGGTAGA GCCACAGGCC
    TCTTTAGAGA ACAAACTGAT GAAGCGCTCA TTCTCCACCA TCGGGGATCA GTGTGTAAAT
    GGTGACTGGC AGGAAGAGTT CTCTCTGGAG AGGGTGAGTC CTGACCGGCT GGAGAGACCC
    AGGCAAAGAA GCTTCAGAGG TGTCAGGTCA AACCTGCGGG AGCCAGTGGA CAAAGAGAGA
    GGACGGTCTA AAGAACGAAG ACACCTGCTG TCTCCAGATG TGTCTCGCTG CAACTCAGAG
    GAAAGGAGTC AGTCTCAGGA GAGACGTCAT TCTCCATCGC CCAGTGAGGG GAGGAAGCAC
    ACAGCTCTCA GACAGGGAAA TAATACAGGC AGCCCTATTG CCTCCACGTC TGATTCAGGG
    ACGCCTCGAG GACGCCGCCA ACTCCCACAG ACACCCTCTC GCCCTCGGCC TCATATCTCC
    TACTCTCCTT TAGTGTGCCG TGCCCATCCA GCACCAGGGG ATGATCGGCC ACGAGCTCCA
    CCCGCAGAGG AAGGAGAGGA GCCCACTGCA ACCGGCCCAG GCACCGCCAT GGCCCACTCT
    ACCCCTCACC GCTACATCTC AGAGCCCTAC CTGCCACTGC AGGAGGACGT CAACAGTCCA
    GACTCCAGTG GTGTGGAGGA GACTCTCACT TTTGAGGCTG CAATAGCAAC CAGCCTGGGC
    CGCTCTAATA CCATTAGCTC GGCGCCACAG CTACGTCACT CCTGGCAGGT GCCTAATGGA
    CATTTCCGCA AACGACTAGC ACAAGCCACA GCTGGGACGT CCGGCTGTGA GCTTCTCAGT
    GACACCGATG AGGATGACCG CTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212915.1
    CDS9..6575
    Translation

    Target ORF information:

    RefSeq Version XM_017357426.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357426.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGATCCTGG CCACCATTAT TGCTAACTGC ATCGTCCTAG CCTTGGAGCA GCACCTGCCT 
    GGAGAAGACA AGACACCCAT GGCTAAGAGA CTTGAAAAAA CAGAGCCCTA CTTCATAGGA
    ATGTTTTGCT TTGAAGCTGG AATAAAAATC ATTGCTTTGG GATTTGTATT CCACAAGGGC
    TCGTACCTGA GGAATGGTTG GAACGTCATG GACTTTATTG TCGTATCGAG TGGGATTCTG
    GCCACAGCAG GAGCCCACAT GAATATTTCA TTAGACCTGC GCACACTGAG GGCTGTGAGG
    GTATTACGGC CCCTCAAACT GGTCTCTGGC ATTCCTAGTC TGCAAATAGT TCTTAAATCC
    ATAATGAAGG CAATGGTGCC ATTGCTTCAG ATCGGACTGC TACTGTTTTT TGCTATTCTA
    ATGTTTGCCA TTATTGGATT AGAGTTCTAC AGTGGGAAAC TCCACAAGCA ATGTGAACCT
    GTAGGTAAAG ATTCTGAGAG AGCAGACTCG CCTGAGAATG AATTTCCATG TGGAAGCAAA
    CTGTGTCCTG ACCATTATAA CTGCTCTGGC TACTGGACGG GACCCAATGA TGGCATCACT
    CAGTTTGATA ACATCTTATT TTCTTTACTC ACCGTCTTCC AGTGCATCAC CATGGAAGGC
    TGGACGACCA TCCTCTATTA CACCAACGAT GCCTTAGGCT CTACTTGGAA TTGGCTCTAC
    TTCATTCCTC TAATCATAAT TGGCTCCTTC TTTGTGTTGA ATCTGGTATT AGGAGTCCTG
    TCAGGGGAGT TTGCTAAAGA GCGAGAGCGG GTAGAGAACC GGAGAGCCTT CATGAAGCTT
    AGGAGACAGC AGCAGATTGA GAGGGAGCTG AACGGATATC GGGCCTGGAT CGACCGTGCA
    GAGGAGGTTA TGCTTGCAGA GGAGAACAAG AATTCAGGAC CTTCTGCTTT GGATGTGCTA
    AAAAGAGCTA CCACCATAAA GAGAAGAGGA GTGGATGATG CTCGAAGAGC TCCAGAAGAG
    CAGTATGCTG ACATCTCATC TCTAGGGATG CCACTGAACA GAGCCAGCAT ACGCAGTGCT
    AAAAGAGGAC CAACATCCTA TTTCCGACGG AAAGAGCGCC TGCTACGGAT CTCCGTCCGC
    CGTATGGTGA AGACTCAGAC GTTCTACTGG ACTGTTCTGG GTCTGGTAGC CCTCAACACT
    CTCTGTGTAG CCATCGTACA TCACAAACAG CCACAGTGGT TATCTAGTCT CCTCTACTAT
    GCAGAGTTTT TGTTTCTGGG TTTGTTTCTT ACCGAGATGT TTCTGAAGAT GTATGGACTC
    GGACCCAGAC TCTATTTTCA CTCCTCCTTC AACTGCTTCG ATTGTGGGGT TATAGTAGGC
    AGCATCTTTG AGGTGTTCTG GGGCTTCTTC AGACCTGGCA CATCCTTTGG GATCAGCGTT
    CTTCGTGCCT TGCGGTTGTT GAGAATTTTC AAGATAACCA AATACTGGGC GTCCCTGAGG
    AACCTGGTCG TGTCTCTGAT GAACTCAATG AAGTCTATTA TCAGTTTGCT CTTCCTGCTT
    TTTCTCTTTA TTGTGGTCTT TGCACTTCTG GGCATGCAGC TCTTTGGTGG AAGGTTCATT
    TTTGATGAGT ACACTCCCAC CAATTTTGAC ACTTTCCCAG CAGCCATCAT GACAGTGTTT
    CAAATTCTGA CTGGTGAGGA CTGGAATGAA GTCATGTACA ATGGCATCCG GTCTCAAGGT
    GGCGTGAAAT CTGGGATGTG GTCGTCTGTC TACTTCATTG TTCTCACTCT GTTTGGAAAT
    TATACGCTGC TTAACGTCTT CTTGGCTATC GCTGTCGACA ATTTGGCCAA TGCCCAGGAG
    CTCACAAAGG ACGAAGAGGA GGAGGAAGAG GCGTTTAATC AGAAACATGC CCTGCAGAAA
    GCCAAGGAGG TTGGGCCCAT GTCCTCTTTC ATGTCCTCAG AGAGGAGGAG GAGGCCCTAC
    CTGTACAGGA AGCGTGCAAT TCATCGGAGC CGTGCAGCCT ACTCCTTCGA GGATGCAGAG
    AGTCTGGCTG ACGCTCGTCC ATTCAATGCC TCTAACTCTT TCATGTCCAG GAGAGAGAGG
    AGGAGGAGGT TGACCATGTC AGTGTGGGAG CAGAGGACCA GCCAGCTGCG GCGGCACAGG
    CAGATGTCCA GCCGCGAGAT CCTTTTCAGC AGCCCCAGTG AAGAACAGGA CGGACCACCA
    GGCTGCCCTC TCCACCGGAA ACCTGGACTA TCTCCATCTG GCAGCAAGCG CAGAGCCACT
    GAGACCACTT CAACCACAAT CATGCCAGAG GGTACCTTAG AGCCACCCAT GCCTATGGAC
    ATGCCGGTGG ATGAGCAAGC TCTGCCCATT TCTATTCCTG AACCCCCTTT AGCCATTCCA
    ATTCCAGAGC CACCAATGCC CGTGGACGCA TGCCTCCCTG AACCCCCAGT TACACTGAAT
    CTGCCCTTAA CAGAGCCATC TGAATCAAAG ACTTCACCCG AGGGTGCTCT GGACATCAAC
    AACCATTGCC CTAGACTAAA TGGGAACAGG CGGCAGCGGG CTGTCCGTAA GCACAGACCT
    CCTGCTATGG GGGACATGCT GACCCCTGAT ATGGGGCCCA GGCCCCGACG GCCACGCCAC
    CGCGAGCTTC ACACAGACCC CAGGGGAAAA GAGACTCTGC AAGGTGATGG TGTTGACGGT
    AAAGAACAAA AGCCAGGAAG TGAGGGTGAT GAAAAGAGTG ATGGAGAAAT CAGTCCAGAT
    GGCAGAGGGC TTGAACAAAG GCAGAACAAA CCAGACAACA CAGAATCATC CACAGAGTCC
    AAACCAGCAA ATTCCCCCCG CAAAATCCCA CCTCTTGACT CTGAACCTGC TACTCTACAG
    CTCACTGACA ACACACTGCA GCAGTCTTCA CAGGGAACTA CATGTGCCGG TGAGACCGAT
    AACAATAACC ACGGGTCCTT GAATCAGGAT AAGTCCTCCC TCAACACCAG CATCGTGATA
    GAGGTCACCG ACACTCAGCC ATCCATGGAG CTGACCTCCA TTACGGGTAA TAGTAAAGAT
    GTGGAGGCCT CAAAGCCAGA GAAAGAAGAA GAAACCCCAG ATGCACCCAA AGGGCCTCCA
    CCTGTCAGCA TGTTCATCTT CAAACCCAAC AACCCGGTCC GCAGGGCCTG CCACTACGTT
    GTCAACCTTC GTTATTTTGA GATGTCAATC CTTCTTGTGA TCGCCGCCAG CAGTATCGCA
    TTGGCTGCCG AAGACCCCGT CAACACTAAT TCGGACAGGA ACAAAGTTCT GCGCTATTTT
    GACTATGTAT TCACTGGGGT CTTCACGTTT GAAATGATAA TCAAGATGAT TGATCAGGGG
    TTGGTCTTAC ACTATGGCTC CTATTTTCGT GACCCGTGGA ACATTCTGGA TTTCATAGTT
    GTGGTGGGAG CTCTGGTGGC TTTTGCTCTC ACGAGTAACA CAGGAAGAGA CATTAAAACC
    ATTAAATCGC TGCGTGTACT CCGAGTTCTG AGGCCTCTCA AGACGATTAA AAGACTTCCT
    AAACTCAAGG CTGTGTTTGA CTGTGTGGTA ACATCACTGA AGAATGTTTT CAACATCCTC
    ATCGTCTACA AGCTGTTTAT GTTCATATTT GCCGTCATTG CCGTGCAGCT TTTCAAAGGG
    AAATTTTACT ACTGTACCGA CAGTTCCAAG GACACTGAAA ACGAGTGCAA AGGCTACTAC
    ATTGACTATG ATAAAGACAA GAAAAAGGAG AAACGAGAGT GGAAGAGACG CGATTTCCAT
    TATGATAACG TTGTCTGGGC TCTGTTGACT CTCTTCACCG TATCCACTGG CGAAGGCTGG
    CCGCAAGTTC TGCAACACTC TGTGGATGTG ACAGAGGAGG ACCGGGGTCC CAGTCAGGGG
    AACAGGATGG AGATGTCTAT CTTCTATGTC ATTTATTTTG TGGTGTTCCC CTTCTTCTTC
    GTCAACATAT TTGTGGCCCT CATCATCATC ACTTTCCAAG AACAGGGTGA CAAAATGATG
    GAGGAGTGTA GTCTGGAGAA AAACGAGAGG GCTTGTATCG ACTTTGCCAT CAGTGCCAAA
    CCTTTGACTC GCTACATGCC CCAGAACAGA CAGACCTTAC AGTACAGACT GTGGCAATTT
    GTGGTGTCGC CCTCATTTGA GTACACAGTA CTGACAATGA TCGCCCTTAA CACCATCGTT
    CTCATGATGA AGCATCATGA TCCTCGTCTT GAAGGCTATG ACGATGTGCT CAAATATCTC
    AACACAATTT TCACCGTTAT TTTCTCTGTT GAGTGTGTTC TCAAGATCCT GGCTTTTGGT
    ATAATGAACT ATTTCAGAGA CACATGGAAT ATATTTGACT TCATCACGGT TCTAGGAAGC
    ATCACAGAAA TTCTGGTGGA CAAATTTCAG ACTGGAAAAA CTTTTAATAT GGGCTTCCTG
    AAGCTATTCC GGGCAGCTCG GCTGATTAAA CTCCTGCGTC AGGGCTACAC CATCCGTATT
    CTGCTCTGGA CTTTTGTTCA GTCCTTCAAG GCTCTACCAT ATGTCTGTCT GCTCATCGCC
    ATGCTGTTTT TCATCTATGC CATCATTGGC ATGCAGGTAT TTGGCAACAT CAAGATAAAT
    GAGAAAACCC AAATCAATGA ACACAATAAC TTCAGGACTT TCTTTGGTGC ACTCATGCTT
    TTGTTCAGGA GTGCAACAGG TGAATCATGG CAGGAAATCA TGCTATCGTG TTTAGGAGAG
    AAGGAGTGTG AAATTGACAC TTCTGTCAAC TCAACAACAA AGACAATAAA AAACGACTGC
    GGTACCGACT TTGCCTACTT CTACTTTGTC TCCTTCATCT TCTTCAGTTC TTTTCTGATG
    CTGAATCTGT TTGTGGCTGT GATCATGGAT AATTTTGAGT ACCTGACACG TGACTCATCC
    ATTCTGGGTC CTCACCACTT AGATGAGTTT GTACGGATCT GGGGAGAGTA TGACCGTGCT
    GCATGTGGCC GTATTCATTA CACTGATATG TACGAGATGC TGACCTGCAT GTCGCCACCG
    CTTGGCCTGG GCAAGAAATG TCCCTCCAAA GTGGCGTATA AGCGGTTGGT TCTGATGAAC
    ATGCCAGTGG ATGAAGACAT GTCTGTCCAC TTCACCTCCA CACTTATGGC CCTCATACGC
    ACCGCACTGG ACATTAAAAT TGCTCGAGGA GGGGAAGACA GGGGTCAGAT GGATGTTGAA
    CTGCAGAAGG AAATCAGTGT CATCTGGCCG CATCTCTCAC AGAAATCTCT TGATCTGCTT
    GTACCCATTC ACAAAGCTAG TGACATGACC ATTGGGAAGA TCTATGCAGC CATGATGATC
    ATGGACTACT ACAAGCAGAA CAAAGCCAAG AAGCTCCGTC AGCAGCTCGA GGAACAGAAA
    CACGCACCAA TGTTCCAACG CATGGATGCA TCCTCGCTGC CGCAGGACAT CCTCTGCAGT
    GCCAAGGCTT TGCCATTCCT GACTCACAGT GCAGGATCTG CCCTCACTAG AGGTGGGTTT
    GTGGCACTGA GTCCTATCTC TCCTCAAGAT CTCTTCATGC AGCCCATGAC CCAAGACAGC
    ATGGAGGAGA GGGAGGAATA CCAACACCCG TATCACCTGC AAAGCATGGT GCCTGAGCAC
    AAAGAATTTA GCAGTGTGAT GTGCCCCACT AGCATGCAGG CTGATCCACT GCAAGTGCCA
    GGAAGAGGCG TCTCTATCCG AGCCTCTTCC ATGCCTCGCC TGGCGGTAGA GCCACAGGCC
    TCTTTAGAGA ACAAACTGAT GAAGCGCTCA TTCTCCACCA TCGGGGATCA GTGTGTAAAT
    GGTGACTGGC AGGAAGAGTT CTCTCTGGAG AGGGTGAGTC CTGACCGGCT GGAGAGACCC
    AGGCAAAGAA GCTTCAGAGG TGTCAGGTCA AACCTGCGGG AGCCAGTGGA CAAAGAGAGA
    GGACGGTCTA AAGAACGAAG ACACCTGCTG TCTCCAGATG TGTCTCGCTG CAACTCAGAG
    GAAAGGAGTC AGTCTCAGGA GAGACGTCAT TCTCCATCGC CCAGTGAGGG GAGGAAGCAC
    ACAGCTCTCA GACAGGGAAA TAATACAGGC AGCCCTATTG CCTCCACGTC TGATTCAGGG
    ACGCCTCGAG GACGCCGCCA ACTCCCACAG ACACCCTCTC GCCCTCGGCC TCATATCTCC
    TACTCTCCTT TAGTGTGCCG TGCCCATCCA GCACCAGGGG ATGATCGGCC ACGAGCTCCA
    CCCGCAGAGG AAGGAGAGGA GCCCACTGCA ACCGGCCCAG GCACCGCCAT GGCCCACTCT
    ACCCCTCACC GCTACATCTC AGAGCCCTAC CTGCCACTGC AGGAGGACGT CAACAGTCCA
    GACTCCAGTG GTGTGGAGGA GACTCTCACT TTTGAGGCTG CAATAGCAAC CAGCCTGGGC
    CGCTCTAATA CCATTAGCTC GGCGCCACAG CTACGTCACT CCTGGCAGGT GCCTAATGGA
    CATTTCCGCA AACGACTAGC ACAAGCCACA GCTGGGACGT CCGGCTGTGA GCTTCTCAGT
    GACACCGATG AGGATGACCG CTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60031
    Clone ID Related Accession (Same CDS sequence) XM_017357427.1
    Accession Version XM_017357427.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6567bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGATCCTGG CCACCATTAT TGCTAACTGC ATCGTCCTAG CCTTGGAGCA GCACCTGCCT 
    GGAGAAGACA AGACACCCAT GGCTAAGAGA CTTGAAAAAA CAGAGCCCTA CTTCATAGGA
    ATGTTTTGCT TTGAAGCTGG AATAAAAATC ATTGCTTTGG GATTTGTATT CCACAAGGGC
    TCGTACCTGA GGAATGGTTG GAACGTCATG GACTTTATTG TCGTATCGAG TGGGATTCTG
    GCCACAGCAG GAGCCCACAT GAATATTTCA TTAGACCTGC GCACACTGAG GGCTGTGAGG
    GTATTACGGC CCCTCAAACT GGTCTCTGGC ATTCCTAGTC TGCAAATAGT TCTTAAATCC
    ATAATGAAGG CAATGGTGCC ATTGCTTCAG ATCGGACTGC TACTGTTTTT TGCTATTCTA
    ATGTTTGCCA TTATTGGATT AGAGTTCTAC AGTGGGAAAC TCCACAAGCA ATGTGAACCT
    GTAGGTAAAG ATTCTGAGAG AGCAGACTCG CCTGAGAATG AATTTCCATG TGGAAGCAAA
    CTGTGTCCTG ACCATTATAA CTGCTCTGGC TACTGGACGG GACCCAATGA TGGCATCACT
    CAGTTTGATA ACATCTTATT TTCTTTACTC ACCGTCTTCC AGTGCATCAC CATGGAAGGC
    TGGACGACCA TCCTCTATTA CACCAACGAT GCCTTAGGCT CTACTTGGAA TTGGCTCTAC
    TTCATTCCTC TAATCATAAT TGGCTCCTTC TTTGTGTTGA ATCTGGTATT AGGAGTCCTG
    TCAGGGGAGT TTGCTAAAGA GCGAGAGCGG GTAGAGAACC GGAGAGCCTT CATGAAGCTT
    AGGAGACAGC AGCAGATTGA GAGGGAGCTG AACGGATATC GGGCCTGGAT CGACCGTGCA
    GAGGAGGTTA TGCTTGCAGA GGAGAACAAG AATTCAGGAC CTTCTGCTTT GGATGTGCTA
    AAAAGAGCTA CCACCATAAA GAGAAGAGGA GTGGATGATG CTCGAAGAGC TCCAGAAGAG
    CAGTATGCTG ACATCTCATC TCTAGGGATG CCACTGAACA GAGCCAGCAT ACGCAGTGCT
    AAAAGAGGAC CAACATCCTA TTTCCGACGG AAAGAGCGCC TGCTACGGAT CTCCGTCCGC
    CGTATGGTGA AGACTCAGAC GTTCTACTGG ACTGTTCTGG GTCTGGTAGC CCTCAACACT
    CTCTGTGTAG CCATCGTACA TCACAAACAG CCACAGTGGT TATCTAGTCT CCTCTACTAT
    GCAGAGTTTT TGTTTCTGGG TTTGTTTCTT ACCGAGATGT TTCTGAAGAT GTATGGACTC
    GGACCCAGAC TCTATTTTCA CTCCTCCTTC AACTGCTTCG ATTGTGGGGT TATAGTAGGC
    AGCATCTTTG AGGTGTTCTG GGGCTTCTTC AGACCTGGCA CATCCTTTGG GATCAGCGTT
    CTTCGTGCCT TGCGGTTGTT GAGAATTTTC AAGATAACCA AATACTGGGC GTCCCTGAGG
    AACCTGGTCG TGTCTCTGAT GAACTCAATG AAGTCTATTA TCAGTTTGCT CTTCCTGCTT
    TTTCTCTTTA TTGTGGTCTT TGCACTTCTG GGCATGCAGC TCTTTGGTGG AAGGTTCATT
    TTTGATGAGT ACACTCCCAC CAATTTTGAC ACTTTCCCAG CAGCCATCAT GACAGTGTTT
    CAAATTCTGA CTGGTGAGGA CTGGAATGAA GTCATGTACA ATGGCATCCG GTCTCAAGGT
    GGCGTGAAAT CTGGGATGTG GTCGTCTGTC TACTTCATTG TTCTCACTCT GTTTGGAAAT
    TATACGCTGC TTAACGTCTT CTTGGCTATC GCTGTCGACA ATTTGGCCAA TGCCCAGGAG
    CTCACAAAGG ACGAAGAGGA GGAGGAAGAG GCGTTTAATC AGAAACATGC CCTGCAGAAA
    GCCAAGGAGG TTGGGCCCAT GTCCTCTTTC ATGTCCTCAG AGAGGAGGAG GAGGCCCTAC
    CTGTACAGGA AGCGTGCAAT TCATCGGAGC CGTGCAGCCT ACTCCTTCGA GGATGCAGAG
    AGTCTGGCTG ACGCTCGTCC ATTCAATGCC TCTAACTCTT TCATGTCCAG GAGAGAGAGG
    AGGAGGAGGT TGACCATGTC AGTGTGGGAG CAGAGGACCA GCCAGCTGCG GCGGCACAGG
    CAGATGTCCA GCCGCGAGAT CCTTTTCAGC AGCCCCAGTG AAGAACAGGA CGGACCACCA
    GGCTGCCCTC TCCACCGGAA ACCTGGACTA TCTCCATCTG GCAGCAAGCG CAGAGCCACT
    GAGACCACTT CAACCACAAT CATGCCAGAG GGTACCTTAG AGCCACCCAT GCCTATGGAC
    ATGCCGGTGG ATGAGCAAGC TCTGCCCATT TCTATTCCTG AACCCCCTTT AGCCATTCCA
    ATTCCAGAGC CACCAATGCC CGTGGACGCA TGCCTCCCTG AACCCCCAGT TACACTGAAT
    CTGCCCTTAA CAGAGCCATC TGAATCAAAG ACTTCACCCG AGGGTGCTCT GGACATCAAC
    AACCATTGCC CTAGACTAAA TGGGAACAGG CGGCAGCGGG CTGTCCGTAA GCACAGACCT
    CCTGCTATGG GGGACATGCT GACCCCTGAT ATGGGGCCCA GGCCCCGACG GCCACGCCAC
    CGCGAGCTTC ACACAGACCC CAGGGGAAAA GAGACTCTGC AAGGTGATGG TGTTGACGGT
    AAAGAACAAA AGCCAGGAAG TGAGGGTGAT GAAAAGAGTG ATGGAGAAAT CAGTCCAGAT
    GGCAGAGGGC TTGAACAAAG GCAGAACAAA CCAGACAACA CAGAATCATC CACAGAGTCC
    AAACCAGCAA ATTCCCCCCG CAAAATCCCA CCTCTTGACT CTGAACCTGC TACTCTACAG
    CTCACTGACA ACACACTGCA GCAGTCTTCA CAGGGAACTA CATGTGCCGG TGAGACCGAT
    AACAATAACC ACGGGTCCTT GAATCAGGAT AAGTCCTCCC TCAACACCAG CATCGTGATA
    GAGGTCACCG ACACTCAGCC ATCCATGGAG CTGACCTCCA TTACGGGTAA TAGTAAAGAT
    GTGGAGGCCT CAAAGCCAGA GAAAGAAGAA GAAACCCCAG ATGCACCCAA AGGGCCTCCA
    CCTGTCAGCA TGTTCATCTT CAAACCCAAC AACCCGGTCC GCAGGGCCTG CCACTACGTT
    GTCAACCTTC GTTATTTTGA GATGTCAATC CTTCTTGTGA TCGCCGCCAG CAGTATCGCA
    TTGGCTGCCG AAGACCCCGT CAACACTAAT TCGGACAGGA ACAAAGTTCT GCGCTATTTT
    GACTATGTAT TCACTGGGGT CTTCACGTTT GAAATGATAA TCAAGATGAT TGATCAGGGG
    TTGGTCTTAC ACTATGGCTC CTATTTTCGT GACCCGTGGA ACATTCTGGA TTTCATAGTT
    GTGGTGGGAG CTCTGGTGGC TTTTGCTCTC ACGAGTAACA CAGGAAGAGA CATTAAAACC
    ATTAAATCGC TGCGTGTACT CCGAGTTCTG AGGCCTCTCA AGACGATTAA AAGACTTCCT
    AAACTCAAGG CTGTGTTTGA CTGTGTGGTA ACATCACTGA AGAATGTTTT CAACATCCTC
    ATCGTCTACA AGCTGTTTAT GTTCATATTT GCCGTCATTG CCGTGCAGCT TTTCAAAGGG
    AAATTTTACT ACTGTACCGA CAGTTCCAAG GACACTGAAA ACGAGTGCAA AGGCTACTAC
    ATTGACTATG ATAAAGACAA GAAAAAGGAG AAACGAGAGT GGAAGAGACG CGATTTCCAT
    TATGATAACG TTGTCTGGGC TCTGTTGACT CTCTTCACCG TATCCACTGG CGAAGGCTGG
    CCGCAAGTTC TGCAACACTC TGTGGATGTG ACAGAGGAGG ACCGGGGTCC CAGTCAGGGG
    AACAGGATGG AGATGTCTAT CTTCTATGTC ATTTATTTTG TGGTGTTCCC CTTCTTCTTC
    GTCAACATAT TTGTGGCCCT CATCATCATC ACTTTCCAAG AACAGGGTGA CAAAATGATG
    GAGGAGTGTA GTCTGGAGAA AAACGAGAGG GCTTGTATCG ACTTTGCCAT CAGTGCCAAA
    CCTTTGACTC GCTACATGCC CCAGAACAGA CAGACCTTAC AGTACAGACT GTGGCAATTT
    GTGGTGTCGC CCTCATTTGA GTACACAGTA CTGACAATGA TCGCCCTTAA CACCATCGTT
    CTCATGATGA AGCATCATGA TCCTCGTCTT GAAGGCTATG ACGATGTGCT CAAATATCTC
    AACACAATTT TCACCGTTAT TTTCTCTGTT GAGTGTGTTC TCAAGATCCT GGCTTTTGGT
    ATAATGAACT ATTTCAGAGA CACATGGAAT ATATTTGACT TCATCACGGT TCTAGGAAGC
    ATCACAGAAA TTCTGGTGGA CAAATTTCAG ACTGGAAAAA CTTTTAATAT GGGCTTCCTG
    AAGCTATTCC GGGCAGCTCG GCTGATTAAA CTCCTGCGTC AGGGCTACAC CATCCGTATT
    CTGCTCTGGA CTTTTGTTCA GTCCTTCAAG GCTCTACCAT ATGTCTGTCT GCTCATCGCC
    ATGCTGTTTT TCATCTATGC CATCATTGGC ATGCAGGTAT TTGGCAACAT CAAGATAAAT
    GAGAAAACCC AAATCAATGA ACACAATAAC TTCAGGACTT TCTTTGGTGC ACTCATGCTT
    TTGTTCAGGA GTGCAACAGG TGAATCATGG CAGGAAATCA TGCTATCGTG TTTAGGAGAG
    AAGGAGTGTG AAATTGACAC TTCTGTCAAC TCAACAACAA AGACAATAAA AAACGACTGC
    GGTACCGACT TTGCCTACTT CTACTTTGTC TCCTTCATCT TCTTCAGTTC TTTTCTGATG
    CTGAATCTGT TTGTGGCTGT GATCATGGAT AATTTTGAGT ACCTGACACG TGACTCATCC
    ATTCTGGGTC CTCACCACTT AGATGAGTTT GTACGGATCT GGGGAGAGTA TGACCGTGCT
    GCATGTGGAA AACTACACTA TAAGGAGATG TACAAAGTAG TACGCACAAT ATCACCTCCT
    CTGGGCTTTG GAAAGAATTG CCCCTACAGG GTGGCATGCA AGCGGTTGGT TCTGATGAAC
    ATGCCAGTGG ATGAAGACAT GTCTGTCCAC TTCACCTCCA CACTTATGGC CCTCATACGC
    ACCGCACTGG ACATTAAAAT TGCTCGAGGA GGGGAAGACA GGGGTCAGAT GGATGTTGAA
    CTGCAGAAGG AAATCAGTGT CATCTGGCCG CATCTCTCAC AGAAATCTCT TGATCTGCTT
    GTACCCATTC ACAAAGCTAG TGACATGACC ATTGGGAAGA TCTATGCAGC CATGATGATC
    ATGGACTACT ACAAGCAGAA CAAAGCCAAG AAGCTCCGTC AGCAGCTCGA GGAACAGAAA
    CACGCACCAA TGTTCCAACG CATGGATGCA TCCTCGCTGC CGCAGGACAT CCTCTGCAGT
    GCCAAGGCTT TGCCATTCCT GACTCACAGT GCAGGATCTG CCCTCACTAG AGGTGGGTTT
    GTGGCACTGA GTCCTATCTC TCCTCAAGAT CTCTTCATGC AGCCCATGAC CCAAGACAGC
    ATGGAGGAGA GGGAGGAATA CCAACACCCG TATCACCTGC AAAGCATGGT GCCTGAGCAC
    AAAGAATTTA GCAGTGTGAT GTGCCCCACT AGCATGCAGG CTGATCCACT GCAAGTGCCA
    GGAAGAGGCG TCTCTATCCG AGCCTCTTCC ATGCCTCGCC TGGCGGTAGA GCCACAGGCC
    TCTTTAGAGA ACAAACTGAT GAAGCGCTCA TTCTCCACCA TCGGGGATCA GTGTGTAAAT
    GGTGACTGGC AGGAAGAGTT CTCTCTGGAG AGGGTGAGTC CTGACCGGCT GGAGAGACCC
    AGGCAAAGAA GCTTCAGAGG TGTCAGGTCA AACCTGCGGG AGCCAGTGGA CAAAGAGAGA
    GGACGGTCTA AAGAACGAAG ACACCTGCTG TCTCCAGATG TGTCTCGCTG CAACTCAGAG
    GAAAGGAGTC AGTCTCAGGA GAGACGTCAT TCTCCATCGC CCAGTGAGGG GAGGAAGCAC
    ACAGCTCTCA GACAGGGAAA TAATACAGGC AGCCCTATTG CCTCCACGTC TGATTCAGGG
    ACGCCTCGAG GACGCCGCCA ACTCCCACAG ACACCCTCTC GCCCTCGGCC TCATATCTCC
    TACTCTCCTT TAGTGTGCCG TGCCCATCCA GCACCAGGGG ATGATCGGCC ACGAGCTCCA
    CCCGCAGAGG AAGGAGAGGA GCCCACTGCA ACCGGCCCAG GCACCGCCAT GGCCCACTCT
    ACCCCTCACC GCTACATCTC AGAGCCCTAC CTGCCACTGC AGGAGGACGT CAACAGTCCA
    GACTCCAGTG GTGTGGAGGA GACTCTCACT TTTGAGGCTG CAATAGCAAC CAGCCTGGGC
    CGCTCTAATA CCATTAGCTC GGCGCCACAG CTACGTCACT CCTGGCAGGT GCCTAATGGA
    CATTTCCGCA AACGACTAGC ACAAGCCACA GCTGGGACGT CCGGCTGTGA GCTTCTCAGT
    GACACCGATG AGGATGACCG CTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212916.1
    CDS9..6575
    Translation

    Target ORF information:

    RefSeq Version XM_017357427.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357427.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGATCCTGG CCACCATTAT TGCTAACTGC ATCGTCCTAG CCTTGGAGCA GCACCTGCCT 
    GGAGAAGACA AGACACCCAT GGCTAAGAGA CTTGAAAAAA CAGAGCCCTA CTTCATAGGA
    ATGTTTTGCT TTGAAGCTGG AATAAAAATC ATTGCTTTGG GATTTGTATT CCACAAGGGC
    TCGTACCTGA GGAATGGTTG GAACGTCATG GACTTTATTG TCGTATCGAG TGGGATTCTG
    GCCACAGCAG GAGCCCACAT GAATATTTCA TTAGACCTGC GCACACTGAG GGCTGTGAGG
    GTATTACGGC CCCTCAAACT GGTCTCTGGC ATTCCTAGTC TGCAAATAGT TCTTAAATCC
    ATAATGAAGG CAATGGTGCC ATTGCTTCAG ATCGGACTGC TACTGTTTTT TGCTATTCTA
    ATGTTTGCCA TTATTGGATT AGAGTTCTAC AGTGGGAAAC TCCACAAGCA ATGTGAACCT
    GTAGGTAAAG ATTCTGAGAG AGCAGACTCG CCTGAGAATG AATTTCCATG TGGAAGCAAA
    CTGTGTCCTG ACCATTATAA CTGCTCTGGC TACTGGACGG GACCCAATGA TGGCATCACT
    CAGTTTGATA ACATCTTATT TTCTTTACTC ACCGTCTTCC AGTGCATCAC CATGGAAGGC
    TGGACGACCA TCCTCTATTA CACCAACGAT GCCTTAGGCT CTACTTGGAA TTGGCTCTAC
    TTCATTCCTC TAATCATAAT TGGCTCCTTC TTTGTGTTGA ATCTGGTATT AGGAGTCCTG
    TCAGGGGAGT TTGCTAAAGA GCGAGAGCGG GTAGAGAACC GGAGAGCCTT CATGAAGCTT
    AGGAGACAGC AGCAGATTGA GAGGGAGCTG AACGGATATC GGGCCTGGAT CGACCGTGCA
    GAGGAGGTTA TGCTTGCAGA GGAGAACAAG AATTCAGGAC CTTCTGCTTT GGATGTGCTA
    AAAAGAGCTA CCACCATAAA GAGAAGAGGA GTGGATGATG CTCGAAGAGC TCCAGAAGAG
    CAGTATGCTG ACATCTCATC TCTAGGGATG CCACTGAACA GAGCCAGCAT ACGCAGTGCT
    AAAAGAGGAC CAACATCCTA TTTCCGACGG AAAGAGCGCC TGCTACGGAT CTCCGTCCGC
    CGTATGGTGA AGACTCAGAC GTTCTACTGG ACTGTTCTGG GTCTGGTAGC CCTCAACACT
    CTCTGTGTAG CCATCGTACA TCACAAACAG CCACAGTGGT TATCTAGTCT CCTCTACTAT
    GCAGAGTTTT TGTTTCTGGG TTTGTTTCTT ACCGAGATGT TTCTGAAGAT GTATGGACTC
    GGACCCAGAC TCTATTTTCA CTCCTCCTTC AACTGCTTCG ATTGTGGGGT TATAGTAGGC
    AGCATCTTTG AGGTGTTCTG GGGCTTCTTC AGACCTGGCA CATCCTTTGG GATCAGCGTT
    CTTCGTGCCT TGCGGTTGTT GAGAATTTTC AAGATAACCA AATACTGGGC GTCCCTGAGG
    AACCTGGTCG TGTCTCTGAT GAACTCAATG AAGTCTATTA TCAGTTTGCT CTTCCTGCTT
    TTTCTCTTTA TTGTGGTCTT TGCACTTCTG GGCATGCAGC TCTTTGGTGG AAGGTTCATT
    TTTGATGAGT ACACTCCCAC CAATTTTGAC ACTTTCCCAG CAGCCATCAT GACAGTGTTT
    CAAATTCTGA CTGGTGAGGA CTGGAATGAA GTCATGTACA ATGGCATCCG GTCTCAAGGT
    GGCGTGAAAT CTGGGATGTG GTCGTCTGTC TACTTCATTG TTCTCACTCT GTTTGGAAAT
    TATACGCTGC TTAACGTCTT CTTGGCTATC GCTGTCGACA ATTTGGCCAA TGCCCAGGAG
    CTCACAAAGG ACGAAGAGGA GGAGGAAGAG GCGTTTAATC AGAAACATGC CCTGCAGAAA
    GCCAAGGAGG TTGGGCCCAT GTCCTCTTTC ATGTCCTCAG AGAGGAGGAG GAGGCCCTAC
    CTGTACAGGA AGCGTGCAAT TCATCGGAGC CGTGCAGCCT ACTCCTTCGA GGATGCAGAG
    AGTCTGGCTG ACGCTCGTCC ATTCAATGCC TCTAACTCTT TCATGTCCAG GAGAGAGAGG
    AGGAGGAGGT TGACCATGTC AGTGTGGGAG CAGAGGACCA GCCAGCTGCG GCGGCACAGG
    CAGATGTCCA GCCGCGAGAT CCTTTTCAGC AGCCCCAGTG AAGAACAGGA CGGACCACCA
    GGCTGCCCTC TCCACCGGAA ACCTGGACTA TCTCCATCTG GCAGCAAGCG CAGAGCCACT
    GAGACCACTT CAACCACAAT CATGCCAGAG GGTACCTTAG AGCCACCCAT GCCTATGGAC
    ATGCCGGTGG ATGAGCAAGC TCTGCCCATT TCTATTCCTG AACCCCCTTT AGCCATTCCA
    ATTCCAGAGC CACCAATGCC CGTGGACGCA TGCCTCCCTG AACCCCCAGT TACACTGAAT
    CTGCCCTTAA CAGAGCCATC TGAATCAAAG ACTTCACCCG AGGGTGCTCT GGACATCAAC
    AACCATTGCC CTAGACTAAA TGGGAACAGG CGGCAGCGGG CTGTCCGTAA GCACAGACCT
    CCTGCTATGG GGGACATGCT GACCCCTGAT ATGGGGCCCA GGCCCCGACG GCCACGCCAC
    CGCGAGCTTC ACACAGACCC CAGGGGAAAA GAGACTCTGC AAGGTGATGG TGTTGACGGT
    AAAGAACAAA AGCCAGGAAG TGAGGGTGAT GAAAAGAGTG ATGGAGAAAT CAGTCCAGAT
    GGCAGAGGGC TTGAACAAAG GCAGAACAAA CCAGACAACA CAGAATCATC CACAGAGTCC
    AAACCAGCAA ATTCCCCCCG CAAAATCCCA CCTCTTGACT CTGAACCTGC TACTCTACAG
    CTCACTGACA ACACACTGCA GCAGTCTTCA CAGGGAACTA CATGTGCCGG TGAGACCGAT
    AACAATAACC ACGGGTCCTT GAATCAGGAT AAGTCCTCCC TCAACACCAG CATCGTGATA
    GAGGTCACCG ACACTCAGCC ATCCATGGAG CTGACCTCCA TTACGGGTAA TAGTAAAGAT
    GTGGAGGCCT CAAAGCCAGA GAAAGAAGAA GAAACCCCAG ATGCACCCAA AGGGCCTCCA
    CCTGTCAGCA TGTTCATCTT CAAACCCAAC AACCCGGTCC GCAGGGCCTG CCACTACGTT
    GTCAACCTTC GTTATTTTGA GATGTCAATC CTTCTTGTGA TCGCCGCCAG CAGTATCGCA
    TTGGCTGCCG AAGACCCCGT CAACACTAAT TCGGACAGGA ACAAAGTTCT GCGCTATTTT
    GACTATGTAT TCACTGGGGT CTTCACGTTT GAAATGATAA TCAAGATGAT TGATCAGGGG
    TTGGTCTTAC ACTATGGCTC CTATTTTCGT GACCCGTGGA ACATTCTGGA TTTCATAGTT
    GTGGTGGGAG CTCTGGTGGC TTTTGCTCTC ACGAGTAACA CAGGAAGAGA CATTAAAACC
    ATTAAATCGC TGCGTGTACT CCGAGTTCTG AGGCCTCTCA AGACGATTAA AAGACTTCCT
    AAACTCAAGG CTGTGTTTGA CTGTGTGGTA ACATCACTGA AGAATGTTTT CAACATCCTC
    ATCGTCTACA AGCTGTTTAT GTTCATATTT GCCGTCATTG CCGTGCAGCT TTTCAAAGGG
    AAATTTTACT ACTGTACCGA CAGTTCCAAG GACACTGAAA ACGAGTGCAA AGGCTACTAC
    ATTGACTATG ATAAAGACAA GAAAAAGGAG AAACGAGAGT GGAAGAGACG CGATTTCCAT
    TATGATAACG TTGTCTGGGC TCTGTTGACT CTCTTCACCG TATCCACTGG CGAAGGCTGG
    CCGCAAGTTC TGCAACACTC TGTGGATGTG ACAGAGGAGG ACCGGGGTCC CAGTCAGGGG
    AACAGGATGG AGATGTCTAT CTTCTATGTC ATTTATTTTG TGGTGTTCCC CTTCTTCTTC
    GTCAACATAT TTGTGGCCCT CATCATCATC ACTTTCCAAG AACAGGGTGA CAAAATGATG
    GAGGAGTGTA GTCTGGAGAA AAACGAGAGG GCTTGTATCG ACTTTGCCAT CAGTGCCAAA
    CCTTTGACTC GCTACATGCC CCAGAACAGA CAGACCTTAC AGTACAGACT GTGGCAATTT
    GTGGTGTCGC CCTCATTTGA GTACACAGTA CTGACAATGA TCGCCCTTAA CACCATCGTT
    CTCATGATGA AGCATCATGA TCCTCGTCTT GAAGGCTATG ACGATGTGCT CAAATATCTC
    AACACAATTT TCACCGTTAT TTTCTCTGTT GAGTGTGTTC TCAAGATCCT GGCTTTTGGT
    ATAATGAACT ATTTCAGAGA CACATGGAAT ATATTTGACT TCATCACGGT TCTAGGAAGC
    ATCACAGAAA TTCTGGTGGA CAAATTTCAG ACTGGAAAAA CTTTTAATAT GGGCTTCCTG
    AAGCTATTCC GGGCAGCTCG GCTGATTAAA CTCCTGCGTC AGGGCTACAC CATCCGTATT
    CTGCTCTGGA CTTTTGTTCA GTCCTTCAAG GCTCTACCAT ATGTCTGTCT GCTCATCGCC
    ATGCTGTTTT TCATCTATGC CATCATTGGC ATGCAGGTAT TTGGCAACAT CAAGATAAAT
    GAGAAAACCC AAATCAATGA ACACAATAAC TTCAGGACTT TCTTTGGTGC ACTCATGCTT
    TTGTTCAGGA GTGCAACAGG TGAATCATGG CAGGAAATCA TGCTATCGTG TTTAGGAGAG
    AAGGAGTGTG AAATTGACAC TTCTGTCAAC TCAACAACAA AGACAATAAA AAACGACTGC
    GGTACCGACT TTGCCTACTT CTACTTTGTC TCCTTCATCT TCTTCAGTTC TTTTCTGATG
    CTGAATCTGT TTGTGGCTGT GATCATGGAT AATTTTGAGT ACCTGACACG TGACTCATCC
    ATTCTGGGTC CTCACCACTT AGATGAGTTT GTACGGATCT GGGGAGAGTA TGACCGTGCT
    GCATGTGGAA AACTACACTA TAAGGAGATG TACAAAGTAG TACGCACAAT ATCACCTCCT
    CTGGGCTTTG GAAAGAATTG CCCCTACAGG GTGGCATGCA AGCGGTTGGT TCTGATGAAC
    ATGCCAGTGG ATGAAGACAT GTCTGTCCAC TTCACCTCCA CACTTATGGC CCTCATACGC
    ACCGCACTGG ACATTAAAAT TGCTCGAGGA GGGGAAGACA GGGGTCAGAT GGATGTTGAA
    CTGCAGAAGG AAATCAGTGT CATCTGGCCG CATCTCTCAC AGAAATCTCT TGATCTGCTT
    GTACCCATTC ACAAAGCTAG TGACATGACC ATTGGGAAGA TCTATGCAGC CATGATGATC
    ATGGACTACT ACAAGCAGAA CAAAGCCAAG AAGCTCCGTC AGCAGCTCGA GGAACAGAAA
    CACGCACCAA TGTTCCAACG CATGGATGCA TCCTCGCTGC CGCAGGACAT CCTCTGCAGT
    GCCAAGGCTT TGCCATTCCT GACTCACAGT GCAGGATCTG CCCTCACTAG AGGTGGGTTT
    GTGGCACTGA GTCCTATCTC TCCTCAAGAT CTCTTCATGC AGCCCATGAC CCAAGACAGC
    ATGGAGGAGA GGGAGGAATA CCAACACCCG TATCACCTGC AAAGCATGGT GCCTGAGCAC
    AAAGAATTTA GCAGTGTGAT GTGCCCCACT AGCATGCAGG CTGATCCACT GCAAGTGCCA
    GGAAGAGGCG TCTCTATCCG AGCCTCTTCC ATGCCTCGCC TGGCGGTAGA GCCACAGGCC
    TCTTTAGAGA ACAAACTGAT GAAGCGCTCA TTCTCCACCA TCGGGGATCA GTGTGTAAAT
    GGTGACTGGC AGGAAGAGTT CTCTCTGGAG AGGGTGAGTC CTGACCGGCT GGAGAGACCC
    AGGCAAAGAA GCTTCAGAGG TGTCAGGTCA AACCTGCGGG AGCCAGTGGA CAAAGAGAGA
    GGACGGTCTA AAGAACGAAG ACACCTGCTG TCTCCAGATG TGTCTCGCTG CAACTCAGAG
    GAAAGGAGTC AGTCTCAGGA GAGACGTCAT TCTCCATCGC CCAGTGAGGG GAGGAAGCAC
    ACAGCTCTCA GACAGGGAAA TAATACAGGC AGCCCTATTG CCTCCACGTC TGATTCAGGG
    ACGCCTCGAG GACGCCGCCA ACTCCCACAG ACACCCTCTC GCCCTCGGCC TCATATCTCC
    TACTCTCCTT TAGTGTGCCG TGCCCATCCA GCACCAGGGG ATGATCGGCC ACGAGCTCCA
    CCCGCAGAGG AAGGAGAGGA GCCCACTGCA ACCGGCCCAG GCACCGCCAT GGCCCACTCT
    ACCCCTCACC GCTACATCTC AGAGCCCTAC CTGCCACTGC AGGAGGACGT CAACAGTCCA
    GACTCCAGTG GTGTGGAGGA GACTCTCACT TTTGAGGCTG CAATAGCAAC CAGCCTGGGC
    CGCTCTAATA CCATTAGCTC GGCGCCACAG CTACGTCACT CCTGGCAGGT GCCTAATGGA
    CATTTCCGCA AACGACTAGC ACAAGCCACA GCTGGGACGT CCGGCTGTGA GCTTCTCAGT
    GACACCGATG AGGATGACCG CTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60032
    Clone ID Related Accession (Same CDS sequence) XM_017357428.1
    Accession Version XM_017357428.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGATCCTGG CCACCATTAT TGCTAACTGC ATCGTCCTAG CCTTGGAGCA GCACCTGCCT 
    GGAGAAGACA AGACACCCAT GGCTAAGAGA CTTGAAAAAA CAGAGCCCTA CTTCATAGGA
    ATGTTTTGCT TTGAAGCTGG AATAAAAATC ATTGCTTTGG GATTTGTATT CCACAAGGGC
    TCGTACCTGA GGAATGGTTG GAACGTCATG GACTTTATTG TCGTATCGAG TGGGATTCTG
    GCCACAGCAG GAGCCCACAT GAATATTTCA TTAGACCTGC GCACACTGAG GGCTGTGAGG
    GTATTACGGC CCCTCAAACT GGTCTCTGGC ATTCCTAGTC TGCAAATAGT TCTTAAATCC
    ATAATGAAGG CAATGGTGCC ATTGCTTCAG ATCGGACTGC TACTGTTTTT TGCTATTCTA
    ATGTTTGCCA TTATTGGATT AGAGTTCTAC AGTGGGAAAC TCCACAAGCA ATGTGAACCT
    GTAGGTAAAG ATTCTGAGAG AGCAGACTCG CCTGAGAATG AATTTCCATG TGGAAGCAAA
    CTGTGTCCTG ACCATTATAA CTGCTCTGGC TACTGGACGG GACCCAATGA TGGCATCACT
    CAGTTTGATA ACATCTTATT TTCTTTACTC ACCGTCTTCC AGTGCATCAC CATGGAAGGC
    TGGACGACCA TCCTCTATTA CACCAACGAT GCCTTAGGCT CTACTTGGAA TTGGCTCTAC
    TTCATTCCTC TAATCATAAT TGGCTCCTTC TTTGTGTTGA ATCTGGTATT AGGAGTCCTG
    TCAGGGGAGT TTGCTAAAGA GCGAGAGCGG GTAGAGAACC GGAGAGCCTT CATGAAGCTT
    AGGAGACAGC AGCAGATTGA GAGGGAGCTG AACGGATATC GGGCCTGGAT CGACCGTGCA
    GAGGAGGTTA TGCTTGCAGA GGAGAACAAG AATTCAGGAC CTTCTGCTTT GGATGTGCTA
    AAAAGAGCTA CCACCATAAA GAGAAGAGGA GTGGATGATG CTCGAAGAGC TCCAGAAGAG
    CAGTATGCTG ACATCTCATC TCTAGGGATG CCACTGAACA GAGCCAGCAT ACGCAGTGCT
    AAAAGAGGAC CAACATCCTA TTTCCGACGG AAAGAGCGCC TGCTACGGAT CTCCGTCCGC
    CGTATGGTGA AGACTCAGAC GTTCTACTGG ACTGTTCTGG GTCTGGTAGC CCTCAACACT
    CTCTGTGTAG CCATCGTACA TCACAAACAG CCACAGTGGT TATCTAGTCT CCTCTACTAT
    GCAGAGTTTT TGTTTCTGGG TTTGTTTCTT ACCGAGATGT TTCTGAAGAT GTATGGACTC
    GGACCCAGAC TCTATTTTCA CTCCTCCTTC AACTGCTTCG ATTGTGGGGT TATAGTAGGC
    AGCATCTTTG AGGTGTTCTG GGGCTTCTTC AGACCTGGCA CATCCTTTGG GATCAGCGTT
    CTTCGTGCCT TGCGGTTGTT GAGAATTTTC AAGATAACCA AATACTGGGC GTCCCTGAGG
    AACCTGGTCG TGTCTCTGAT GAACTCAATG AAGTCTATTA TCAGTTTGCT CTTCCTGCTT
    TTTCTCTTTA TTGTGGTCTT TGCACTTCTG GGCATGCAGC TCTTTGGTGG AAGGTTCATT
    TTTGATGAGT ACACTCCCAC CAATTTTGAC ACTTTCCCAG CAGCCATCAT GACAGTGTTT
    CAAATTCTGA CTGGTGAGGA CTGGAATGAA GTCATGTACA ATGGCATCCG GTCTCAAGGT
    GGCGTGAAAT CTGGGATGTG GTCGTCTGTC TACTTCATTG TTCTCACTCT GTTTGGAAAT
    TATACGCTGC TTAACGTCTT CTTGGCTATC GCTGTCGACA ATTTGGCCAA TGCCCAGGAG
    CTCACAAAGG ACGAAGAGGA GGAGGAAGAG GCGTTTAATC AGAAACATGC CCTGCAGAAA
    GCCAAGGAGG TTGGGCCCAT GTCCTCTTTC ATGTCCTCAG AGAGGAGGAG GAGGCCCTAC
    CTGTACAGGA AGCGTGCAAT TCATCGGAGC CGTGCAGCCT ACTCCTTCGA GGATGCAGAG
    AGTCTGGCTG ACGCTCGTCC ATTCAATGCC TCTAACTCTT TCATGTCCAG GAGAGAGAGG
    AGGAGGAGGT TGACCATGTC AGTGTGGGAG CAGAGGACCA GCCAGCTGCG GCGGCACAGG
    CAGATGTCCA GCCGCGAGAT CCTTTTCAGC AGCCCCAGTG AAGAACAGGA CGGACCACCA
    GGCTGCCCTC TCCACCGGAA ACCTGGACTA TCTCCATCTG GCAGCAAGCG CAGAGCCACT
    GAGACCACTT CAACCACAAT CATGCCAGAG GGTACCTTAG AGCCACCCAT GCCTATGGAC
    ATGCCGGTGG ATGAGCAAGC TCTGCCCATT TCTATTCCTG AACCCCCTTT AGCCATTCCA
    ATTCCAGAGC CACCAATGCC CGTGGACGCA TGCCTCCCTG AACCCCCAGT TACACTGAAT
    CTGCCCTTAA CAGAGCCATC TGAATCAAAG ACTTCACCCG AGGGTGCTCT GGACATCAAC
    AACCATTGCC CTAGACTAAA TGGGAACAGG CGGCAGCGGG CTGTCCGTAA GCACAGACCT
    CCTGCTATGG GGGACATGCT GACCCCTGAT ATGGGGCCCA GGCCCCGACG GCCACGCCAC
    CGCGAGCTTC ACACAGACCC CAGGGGAAAA GAGACTCTGC AAGGTGATGG TGTTGACGGT
    AAAGAACAAA AGCCAGGAAG TGAGGGTGAT GAAAAGAGTG ATGGAGAAAT CAGTCCAGAT
    GGCAGAGGGC TTGAACAAAG GCAGAACAAA CCAGACAACA CAGAATCATC CACAGAGTCC
    AAACCAGCAA ATTCCCCCCG CAAAATCCCA CCTCTTGACT CTGAACCTGC TACTCTACAG
    CTCACTGACA ACACACTGCA GCAGTCTTCA CAGGGAACTA CATGTGCCGG TGAGACCGAT
    AACAATAACC ACGGGTCCTT GAATCAGGAT AAGTCCTCCC TCAACACCAG CATCGTGATA
    GAGGTCACCG ACACTCAGCC ATCCATGGAG CTGACCTCCA TTACGGGTAA TAGTAAAGAT
    GTGGAGGCCT CAAAGCCAGA GAAAGAAGAA GAAACCCCAG ATGCACCCAA AGGGCCTCCA
    CCTGTCAGCA TGTTCATCTT CAAACCCAAC AACCCGGTCC GCAGGGCCTG CCACTACGTT
    GTCAACCTTC GTTATTTTGA GATGTCAATC CTTCTTGTGA TCGCCGCCAG CAGTATCGCA
    TTGGCTGCCG AAGACCCCGT CAACACTAAT TCGGACAGGA ACAAAGTTCT GCGCTATTTT
    GACTATGTAT TCACTGGGGT CTTCACGTTT GAAATGATAA TCAAGATGAT TGATCAGGGG
    TTGGTCTTAC ACTATGGCTC CTATTTTCGT GACCCGTGGA ACATTCTGGA TTTCATAGTT
    GTGGTGGGAG CTCTGGTGGC TTTTGCTCTC ACGAATGTGA TGGGGAGTAA CACAGGAAGA
    GACATTAAAA CCATTAAATC GCTGCGTGTA CTCCGAGTTC TGAGGCCTCT CAAGACGATT
    AAAAGACTTC CTAAACTCAA GGCTGTGTTT GACTGTGTGG TAACATCACT GAAGAATGTT
    TTCAACATCC TCATCGTCTA CAAGCTGTTT ATGTTCATAT TTGCCGTCAT TGCCGTGCAG
    CTTTTCAAAG GGAAATTTTA CTACTGTACC GACAGTTCCA AGGACACTGA AAACGAGTGC
    AAAGGCTACT ACATTGACTA TGATAAAGAC AAGAAAAAGG AGAAACGAGA GTGGAAGAGA
    CGCGATTTCC ATTATGATAA CGTTGTCTGG GCTCTGTTGA CTCTCTTCAC CGTATCCACT
    GGCGAAGGCT GGCCGCAAGT TCTGCAACAC TCTGTGGATG TGACAGAGGA GGACCGGGGT
    CCCAGTCAGG GGAACAGGAT GGAGATGTCT ATCTTCTATG TCATTTATTT TGTGGTGTTC
    CCCTTCTTCT TCGTCAACAT ATTTGTGGCC CTCATCATCA TCACTTTCCA AGAACAGGGT
    GACAAAATGA TGGAGGAGTG TAGTCTGGAG AAAAACGAGA GGGCTTGTAT CGACTTTGCC
    ATCAGTGCCA AACCTTTGAC TCGCTACATG CCCCAGAACA GACAGACCTT ACAGTACAGA
    CTGTGGCAAT TTGTGGTGTC GCCCTCATTT GAGTACACAG TACTGACAAT GATCGCCCTT
    AACACCATCG TTCTCATGAT GAAGCATCAT GATCCTCGTC TTGAAGGCTA TGACGATGTG
    CTCAAATATC TCAACACAAT TTTCACCGTT ATTTTCTCTG TTGAGTGTGT TCTCAAGATC
    CTGGCTTTTG GTATAATGAA CTATTTCAGA GACACATGGA ATATATTTGA CTTCATCACG
    GTTCTAGGAA GCATCACAGA AATTCTGGTG GACAAATTTC AGACTGGAAA AACTTTTAAT
    ATGGGCTTCC TGAAGCTATT CCGGGCAGCT CGGCTGATTA AACTCCTGCG TCAGGGCTAC
    ACCATCCGTA TTCTGCTCTG GACTTTTGTT CAGTCCTTCA AGGCTCTACC ATATGTCTGT
    CTGCTCATCG CCATGCTGTT TTTCATCTAT GCCATCATTG GCATGCAGGT ATTTGGCAAC
    ATCAAGATAA ATGAGAAAAC CCAAATCAAT GAACACAATA ACTTCAGGAC TTTCTTTGGT
    GCACTCATGC TTTTGTTCAG GAGTGCAACA GGTGAATCAT GGCAGGAAAT CATGCTATCG
    TGTTTAGGAG AGAAGGAGTG TGAAATTGAC ACTTCTGTCA ACTCAACAAC AAAGACAATA
    AAAAACGACT GCGGTACCGA CTTTGCCTAC TTCTACTTTG TCTCCTTCAT CTTCTTCAGT
    TCTTTTCTGA TGCTGAATCT GTTTGTGGCT GTGATCATGG ATAATTTTGA GTACCTGACA
    CGTGACTCAT CCATTCTGGG TCCTCACCAC TTAGATGAGT TTGTACGGAT CTGGGGAGAG
    TATGACCGTG CTGCATGTGG CCGTATTCAT TACACTGATA TGTACGAGAT GCTGACCTGC
    ATGTCGCCAC CGCTTGGCCT GGGCAAGAAA TGTCCCTCCA AAGTGGCGTA TAAGCGGTTG
    GTTCTGATGA ACATGCCAGT GGATGAAGAC ATGTCTGTCC ACTTCACCTC CACACTTATG
    GCCCTCATAC GCACCGCACT GGACATTAAA ATTGCTCGAG GAGGGGAAGA CAGGGGTCAG
    ATGGATGTTG AACTGCAGAA GGAAATCAGT GTCATCTGGC CGCATCTCTC ACAGAAATCT
    CTTGATCTGC TTGTACCCAT TCACAAAGCT AGTGACATGA CCATTGGGAA GATCTATGCA
    GCCATGATGA TCATGGACTA CTACAAGCAG AACAAAGCCA AGAAGCTCCG TCAGCAGCTC
    GAGGAACAGA AACACGCACC AATGTTCCAA CGCATGGATG CATCCTCGCT GCCGCAGGAC
    ATCCTCTGCA GTGCCAAGGC TTTGCCATTC CTGACTCACA GTGCAGGATC TGCCCTCACT
    AGAGGTGGGT TTGTGGCACT GAGTCCTATC TCTCCTCAAG ATCTCTTCAT GCAGCCCATG
    ACCCAAGACA GCATGGAGGA GAGGGAGGAA TACCAACACC CGTATCACCT GCAAAGCATG
    GCCTCTTTAG AGAACAAACT GATGAAGCGC TCATTCTCCA CCATCGGGGA TCAGTGTGTA
    AATGGTGACT GGCAGGAAGA GTTCTCTCTG GAGAGGGTGA GTCCTGACCG GCTGGAGAGA
    CCCAGGCAAA GAAGCTTCAG AGGTGTCAGG TCAAACCTGC GGGAGCCAGT GGACAAAGAG
    AGAGGACGGT CTAAAGAACG AAGACACCTG CTGTCTCCAG ATGTGTCTCG CTGCAACTCA
    GAGGAAAGGA GTCAGTCTCA GGAGAGACGT CATTCTCCAT CGCCCAGTGA GGGGAGGAAG
    CACACAGCTC TCAGACAGGG AAATAATACA GGCAGCCCTA TTGCCTCCAC GTCTGATTCA
    GGGACGCCTC GAGGACGCCG CCAACTCCCA CAGACACCCT CTCGCCCTCG GCCTCATATC
    TCCTACTCTC CTTTAGTGTG CCGTGCCCAT CCAGCACCAG GGGATGATCG GCCACGAGCT
    CCACCCGCAG AGGAAGGAGA GGAGCCCACT GCAACCGGCC CAGGCACCGC CATGGCCCAC
    TCTACCCCTC ACCGCTACAT CTCAGAGCCC TACCTGCCAC TGCAGGAGGA CGTCAACAGT
    CCAGACTCCA GTGGTGTGGA GGAGACTCTC ACTTTTGAGG CTGCAATAGC AACCAGCCTG
    GGCCGCTCTA ATACCATTAG CTCGGCGCCA CAGCTACGTC ACTCCTGGCA GGTGCCTAAT
    GGACATTTCC GCAAACGACT AGCACAAGCC ACAGCTGGGA CGTCCGGCTG TGAGCTTCTC
    AGTGACACCG ATGAGGATGA CCGCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212917.1
    CDS9..6458
    Translation

    Target ORF information:

    RefSeq Version XM_017357428.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium voltage-gated channel subunit alpha1 E (cacna1e), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357428.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGATCCTGG CCACCATTAT TGCTAACTGC ATCGTCCTAG CCTTGGAGCA GCACCTGCCT 
    GGAGAAGACA AGACACCCAT GGCTAAGAGA CTTGAAAAAA CAGAGCCCTA CTTCATAGGA
    ATGTTTTGCT TTGAAGCTGG AATAAAAATC ATTGCTTTGG GATTTGTATT CCACAAGGGC
    TCGTACCTGA GGAATGGTTG GAACGTCATG GACTTTATTG TCGTATCGAG TGGGATTCTG
    GCCACAGCAG GAGCCCACAT GAATATTTCA TTAGACCTGC GCACACTGAG GGCTGTGAGG
    GTATTACGGC CCCTCAAACT GGTCTCTGGC ATTCCTAGTC TGCAAATAGT TCTTAAATCC
    ATAATGAAGG CAATGGTGCC ATTGCTTCAG ATCGGACTGC TACTGTTTTT TGCTATTCTA
    ATGTTTGCCA TTATTGGATT AGAGTTCTAC AGTGGGAAAC TCCACAAGCA ATGTGAACCT
    GTAGGTAAAG ATTCTGAGAG AGCAGACTCG CCTGAGAATG AATTTCCATG TGGAAGCAAA
    CTGTGTCCTG ACCATTATAA CTGCTCTGGC TACTGGACGG GACCCAATGA TGGCATCACT
    CAGTTTGATA ACATCTTATT TTCTTTACTC ACCGTCTTCC AGTGCATCAC CATGGAAGGC
    TGGACGACCA TCCTCTATTA CACCAACGAT GCCTTAGGCT CTACTTGGAA TTGGCTCTAC
    TTCATTCCTC TAATCATAAT TGGCTCCTTC TTTGTGTTGA ATCTGGTATT AGGAGTCCTG
    TCAGGGGAGT TTGCTAAAGA GCGAGAGCGG GTAGAGAACC GGAGAGCCTT CATGAAGCTT
    AGGAGACAGC AGCAGATTGA GAGGGAGCTG AACGGATATC GGGCCTGGAT CGACCGTGCA
    GAGGAGGTTA TGCTTGCAGA GGAGAACAAG AATTCAGGAC CTTCTGCTTT GGATGTGCTA
    AAAAGAGCTA CCACCATAAA GAGAAGAGGA GTGGATGATG CTCGAAGAGC TCCAGAAGAG
    CAGTATGCTG ACATCTCATC TCTAGGGATG CCACTGAACA GAGCCAGCAT ACGCAGTGCT
    AAAAGAGGAC CAACATCCTA TTTCCGACGG AAAGAGCGCC TGCTACGGAT CTCCGTCCGC
    CGTATGGTGA AGACTCAGAC GTTCTACTGG ACTGTTCTGG GTCTGGTAGC CCTCAACACT
    CTCTGTGTAG CCATCGTACA TCACAAACAG CCACAGTGGT TATCTAGTCT CCTCTACTAT
    GCAGAGTTTT TGTTTCTGGG TTTGTTTCTT ACCGAGATGT TTCTGAAGAT GTATGGACTC
    GGACCCAGAC TCTATTTTCA CTCCTCCTTC AACTGCTTCG ATTGTGGGGT TATAGTAGGC
    AGCATCTTTG AGGTGTTCTG GGGCTTCTTC AGACCTGGCA CATCCTTTGG GATCAGCGTT
    CTTCGTGCCT TGCGGTTGTT GAGAATTTTC AAGATAACCA AATACTGGGC GTCCCTGAGG
    AACCTGGTCG TGTCTCTGAT GAACTCAATG AAGTCTATTA TCAGTTTGCT CTTCCTGCTT
    TTTCTCTTTA TTGTGGTCTT TGCACTTCTG GGCATGCAGC TCTTTGGTGG AAGGTTCATT
    TTTGATGAGT ACACTCCCAC CAATTTTGAC ACTTTCCCAG CAGCCATCAT GACAGTGTTT
    CAAATTCTGA CTGGTGAGGA CTGGAATGAA GTCATGTACA ATGGCATCCG GTCTCAAGGT
    GGCGTGAAAT CTGGGATGTG GTCGTCTGTC TACTTCATTG TTCTCACTCT GTTTGGAAAT
    TATACGCTGC TTAACGTCTT CTTGGCTATC GCTGTCGACA ATTTGGCCAA TGCCCAGGAG
    CTCACAAAGG ACGAAGAGGA GGAGGAAGAG GCGTTTAATC AGAAACATGC CCTGCAGAAA
    GCCAAGGAGG TTGGGCCCAT GTCCTCTTTC ATGTCCTCAG AGAGGAGGAG GAGGCCCTAC
    CTGTACAGGA AGCGTGCAAT TCATCGGAGC CGTGCAGCCT ACTCCTTCGA GGATGCAGAG
    AGTCTGGCTG ACGCTCGTCC ATTCAATGCC TCTAACTCTT TCATGTCCAG GAGAGAGAGG
    AGGAGGAGGT TGACCATGTC AGTGTGGGAG CAGAGGACCA GCCAGCTGCG GCGGCACAGG
    CAGATGTCCA GCCGCGAGAT CCTTTTCAGC AGCCCCAGTG AAGAACAGGA CGGACCACCA
    GGCTGCCCTC TCCACCGGAA ACCTGGACTA TCTCCATCTG GCAGCAAGCG CAGAGCCACT
    GAGACCACTT CAACCACAAT CATGCCAGAG GGTACCTTAG AGCCACCCAT GCCTATGGAC
    ATGCCGGTGG ATGAGCAAGC TCTGCCCATT TCTATTCCTG AACCCCCTTT AGCCATTCCA
    ATTCCAGAGC CACCAATGCC CGTGGACGCA TGCCTCCCTG AACCCCCAGT TACACTGAAT
    CTGCCCTTAA CAGAGCCATC TGAATCAAAG ACTTCACCCG AGGGTGCTCT GGACATCAAC
    AACCATTGCC CTAGACTAAA TGGGAACAGG CGGCAGCGGG CTGTCCGTAA GCACAGACCT
    CCTGCTATGG GGGACATGCT GACCCCTGAT ATGGGGCCCA GGCCCCGACG GCCACGCCAC
    CGCGAGCTTC ACACAGACCC CAGGGGAAAA GAGACTCTGC AAGGTGATGG TGTTGACGGT
    AAAGAACAAA AGCCAGGAAG TGAGGGTGAT GAAAAGAGTG ATGGAGAAAT CAGTCCAGAT
    GGCAGAGGGC TTGAACAAAG GCAGAACAAA CCAGACAACA CAGAATCATC CACAGAGTCC
    AAACCAGCAA ATTCCCCCCG CAAAATCCCA CCTCTTGACT CTGAACCTGC TACTCTACAG
    CTCACTGACA ACACACTGCA GCAGTCTTCA CAGGGAACTA CATGTGCCGG TGAGACCGAT
    AACAATAACC ACGGGTCCTT GAATCAGGAT AAGTCCTCCC TCAACACCAG CATCGTGATA
    GAGGTCACCG ACACTCAGCC ATCCATGGAG CTGACCTCCA TTACGGGTAA TAGTAAAGAT
    GTGGAGGCCT CAAAGCCAGA GAAAGAAGAA GAAACCCCAG ATGCACCCAA AGGGCCTCCA
    CCTGTCAGCA TGTTCATCTT CAAACCCAAC AACCCGGTCC GCAGGGCCTG CCACTACGTT
    GTCAACCTTC GTTATTTTGA GATGTCAATC CTTCTTGTGA TCGCCGCCAG CAGTATCGCA
    TTGGCTGCCG AAGACCCCGT CAACACTAAT TCGGACAGGA ACAAAGTTCT GCGCTATTTT
    GACTATGTAT TCACTGGGGT CTTCACGTTT GAAATGATAA TCAAGATGAT TGATCAGGGG
    TTGGTCTTAC ACTATGGCTC CTATTTTCGT GACCCGTGGA ACATTCTGGA TTTCATAGTT
    GTGGTGGGAG CTCTGGTGGC TTTTGCTCTC ACGAATGTGA TGGGGAGTAA CACAGGAAGA
    GACATTAAAA CCATTAAATC GCTGCGTGTA CTCCGAGTTC TGAGGCCTCT CAAGACGATT
    AAAAGACTTC CTAAACTCAA GGCTGTGTTT GACTGTGTGG TAACATCACT GAAGAATGTT
    TTCAACATCC TCATCGTCTA CAAGCTGTTT ATGTTCATAT TTGCCGTCAT TGCCGTGCAG
    CTTTTCAAAG GGAAATTTTA CTACTGTACC GACAGTTCCA AGGACACTGA AAACGAGTGC
    AAAGGCTACT ACATTGACTA TGATAAAGAC AAGAAAAAGG AGAAACGAGA GTGGAAGAGA
    CGCGATTTCC ATTATGATAA CGTTGTCTGG GCTCTGTTGA CTCTCTTCAC CGTATCCACT
    GGCGAAGGCT GGCCGCAAGT TCTGCAACAC TCTGTGGATG TGACAGAGGA GGACCGGGGT
    CCCAGTCAGG GGAACAGGAT GGAGATGTCT ATCTTCTATG TCATTTATTT TGTGGTGTTC
    CCCTTCTTCT TCGTCAACAT ATTTGTGGCC CTCATCATCA TCACTTTCCA AGAACAGGGT
    GACAAAATGA TGGAGGAGTG TAGTCTGGAG AAAAACGAGA GGGCTTGTAT CGACTTTGCC
    ATCAGTGCCA AACCTTTGAC TCGCTACATG CCCCAGAACA GACAGACCTT ACAGTACAGA
    CTGTGGCAAT TTGTGGTGTC GCCCTCATTT GAGTACACAG TACTGACAAT GATCGCCCTT
    AACACCATCG TTCTCATGAT GAAGCATCAT GATCCTCGTC TTGAAGGCTA TGACGATGTG
    CTCAAATATC TCAACACAAT TTTCACCGTT ATTTTCTCTG TTGAGTGTGT TCTCAAGATC
    CTGGCTTTTG GTATAATGAA CTATTTCAGA GACACATGGA ATATATTTGA CTTCATCACG
    GTTCTAGGAA GCATCACAGA AATTCTGGTG GACAAATTTC AGACTGGAAA AACTTTTAAT
    ATGGGCTTCC TGAAGCTATT CCGGGCAGCT CGGCTGATTA AACTCCTGCG TCAGGGCTAC
    ACCATCCGTA TTCTGCTCTG GACTTTTGTT CAGTCCTTCA AGGCTCTACC ATATGTCTGT
    CTGCTCATCG CCATGCTGTT TTTCATCTAT GCCATCATTG GCATGCAGGT ATTTGGCAAC
    ATCAAGATAA ATGAGAAAAC CCAAATCAAT GAACACAATA ACTTCAGGAC TTTCTTTGGT
    GCACTCATGC TTTTGTTCAG GAGTGCAACA GGTGAATCAT GGCAGGAAAT CATGCTATCG
    TGTTTAGGAG AGAAGGAGTG TGAAATTGAC ACTTCTGTCA ACTCAACAAC AAAGACAATA
    AAAAACGACT GCGGTACCGA CTTTGCCTAC TTCTACTTTG TCTCCTTCAT CTTCTTCAGT
    TCTTTTCTGA TGCTGAATCT GTTTGTGGCT GTGATCATGG ATAATTTTGA GTACCTGACA
    CGTGACTCAT CCATTCTGGG TCCTCACCAC TTAGATGAGT TTGTACGGAT CTGGGGAGAG
    TATGACCGTG CTGCATGTGG CCGTATTCAT TACACTGATA TGTACGAGAT GCTGACCTGC
    ATGTCGCCAC CGCTTGGCCT GGGCAAGAAA TGTCCCTCCA AAGTGGCGTA TAAGCGGTTG
    GTTCTGATGA ACATGCCAGT GGATGAAGAC ATGTCTGTCC ACTTCACCTC CACACTTATG
    GCCCTCATAC GCACCGCACT GGACATTAAA ATTGCTCGAG GAGGGGAAGA CAGGGGTCAG
    ATGGATGTTG AACTGCAGAA GGAAATCAGT GTCATCTGGC CGCATCTCTC ACAGAAATCT
    CTTGATCTGC TTGTACCCAT TCACAAAGCT AGTGACATGA CCATTGGGAA GATCTATGCA
    GCCATGATGA TCATGGACTA CTACAAGCAG AACAAAGCCA AGAAGCTCCG TCAGCAGCTC
    GAGGAACAGA AACACGCACC AATGTTCCAA CGCATGGATG CATCCTCGCT GCCGCAGGAC
    ATCCTCTGCA GTGCCAAGGC TTTGCCATTC CTGACTCACA GTGCAGGATC TGCCCTCACT
    AGAGGTGGGT TTGTGGCACT GAGTCCTATC TCTCCTCAAG ATCTCTTCAT GCAGCCCATG
    ACCCAAGACA GCATGGAGGA GAGGGAGGAA TACCAACACC CGTATCACCT GCAAAGCATG
    GCCTCTTTAG AGAACAAACT GATGAAGCGC TCATTCTCCA CCATCGGGGA TCAGTGTGTA
    AATGGTGACT GGCAGGAAGA GTTCTCTCTG GAGAGGGTGA GTCCTGACCG GCTGGAGAGA
    CCCAGGCAAA GAAGCTTCAG AGGTGTCAGG TCAAACCTGC GGGAGCCAGT GGACAAAGAG
    AGAGGACGGT CTAAAGAACG AAGACACCTG CTGTCTCCAG ATGTGTCTCG CTGCAACTCA
    GAGGAAAGGA GTCAGTCTCA GGAGAGACGT CATTCTCCAT CGCCCAGTGA GGGGAGGAAG
    CACACAGCTC TCAGACAGGG AAATAATACA GGCAGCCCTA TTGCCTCCAC GTCTGATTCA
    GGGACGCCTC GAGGACGCCG CCAACTCCCA CAGACACCCT CTCGCCCTCG GCCTCATATC
    TCCTACTCTC CTTTAGTGTG CCGTGCCCAT CCAGCACCAG GGGATGATCG GCCACGAGCT
    CCACCCGCAG AGGAAGGAGA GGAGCCCACT GCAACCGGCC CAGGCACCGC CATGGCCCAC
    TCTACCCCTC ACCGCTACAT CTCAGAGCCC TACCTGCCAC TGCAGGAGGA CGTCAACAGT
    CCAGACTCCA GTGGTGTGGA GGAGACTCTC ACTTTTGAGG CTGCAATAGC AACCAGCCTG
    GGCCGCTCTA ATACCATTAG CTCGGCGCCA CAGCTACGTC ACTCCTGGCA GGTGCCTAAT
    GGACATTTCC GCAAACGACT AGCACAAGCC ACAGCTGGGA CGTCCGGCTG TGAGCTTCTC
    AGTGACACCG ATGAGGATGA CCGCTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.