CACNA1E cDNA ORF clone, Gallus gallus(chicken)

The following CACNA1E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa152514 XM_015290485.2
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa152515 XM_015290486.2
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa39019 XM_015290485.1
Latest version!
Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa39020 XM_015290486.1
Latest version!
Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa152506 XM_015290476.2
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OGa152507 XM_015290478.2
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OGa152508 XM_015290479.2
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OGa152509 XM_015290480.2
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OGa152510 XM_015290481.2
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OGa152511 XM_015290482.2
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OGa152512 XM_015290483.2
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OGa152513 XM_015290484.2
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa152516 XM_015290487.2
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OGa152517 XM_015290488.2
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OGa152518 XM_025153110.1
Latest version!
Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OGa39011 XM_015290476.1
Latest version!
Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa39011 XM_015290477.1
Latest version!
Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa39012 XM_015290478.1
Latest version!
Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa39013 XM_015290479.1
Latest version!
Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa39014 XM_015290480.1
Latest version!
Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa39015 XM_015290481.1
Latest version!
Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa39016 XM_015290482.1
Latest version!
Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa39017 XM_015290483.1
Latest version!
Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa39018 XM_015290484.1
Latest version!
Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa39021 XM_015290487.1
Latest version!
Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa39022 XM_015290488.1
Latest version!
Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa152514
    Clone ID Related Accession (Same CDS sequence) XM_015290485.2
    Accession Version XM_015290485.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5781bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATTTTTGTGG CTCTCATCAT CATCACATTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CGGTCATCAT GGACAATTTT
    GAGTACCTGA CTCGAGATTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGT GGCCGAATCC ATTACACTGA GATGTATGAA
    ATGCTGACTC TTATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCTTC CAAAGTGGCC
    TATAAGCCTG GTCGATGTGT TTTTCGATAT TGTTTTCCGT CCGCTGTTTT CTTCTCTCTC
    TTGCGCAGAG ACTGGTGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145971.2
    CDS129..5909
    Translation

    Target ORF information:

    RefSeq Version XM_015290485.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290485.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATTTTTGTGG CTCTCATCAT CATCACATTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CGGTCATCAT GGACAATTTT
    GAGTACCTGA CTCGAGATTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGT GGCCGAATCC ATTACACTGA GATGTATGAA
    ATGCTGACTC TTATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCTTC CAAAGTGGCC
    TATAAGCCTG GTCGATGTGT TTTTCGATAT TGTTTTCCGT CCGCTGTTTT CTTCTCTCTC
    TTGCGCAGAG ACTGGTGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152515
    Clone ID Related Accession (Same CDS sequence) XM_015290486.2
    Accession Version XM_015290486.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5610bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290486.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATTTTTGTGG CTCTCATCAT CATCACATTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CGGTCATCAT GGACAATTTT
    GAGTACCTGA CTCGAGATTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145972.2
    CDS129..5738
    Translation

    Target ORF information:

    RefSeq Version XM_015290486.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290486.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATTTTTGTGG CTCTCATCAT CATCACATTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CGGTCATCAT GGACAATTTT
    GAGTACCTGA CTCGAGATTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39019
    Clone ID Related Accession (Same CDS sequence) XM_015290485.1
    Accession Version XM_015290485.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5781bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATCTTTGTGG CGTTCATCAT CATCACCTTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT
    GAGTACCTGA CTCGAGACTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGT GGCCGAATCC ATTACACTGA GATGTATGAA
    ATGCTGACTC TTATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCTTC CAAAGTGGCC
    TATAAGCCTG GTCGATGTGT TTTTCGATAT TGTTTTCCGT CCGCTGTTTT CTTCTCTCTC
    TTGCGCAGAG ACTGGTGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145971.1
    CDS123..5903
    Translation

    Target ORF information:

    RefSeq Version XM_015290485.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290485.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATCTTTGTGG CGTTCATCAT CATCACCTTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT
    GAGTACCTGA CTCGAGACTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGT GGCCGAATCC ATTACACTGA GATGTATGAA
    ATGCTGACTC TTATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCTTC CAAAGTGGCC
    TATAAGCCTG GTCGATGTGT TTTTCGATAT TGTTTTCCGT CCGCTGTTTT CTTCTCTCTC
    TTGCGCAGAG ACTGGTGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39020
    Clone ID Related Accession (Same CDS sequence) XM_015290486.1
    Accession Version XM_015290486.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5610bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATCTTTGTGG CGTTCATCAT CATCACCTTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT
    GAGTACCTGA CTCGAGACTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145972.1
    CDS123..5732
    Translation

    Target ORF information:

    RefSeq Version XM_015290486.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290486.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATCTTTGTGG CGTTCATCAT CATCACCTTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT
    GAGTACCTGA CTCGAGACTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152506
    Clone ID Related Accession (Same CDS sequence) XM_015290476.2
    Accession Version XM_015290476.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290476.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATTTTTGTGG CTCTCATCAT CATCACATTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CGGTCATCAT GGACAATTTT
    GAGTACCTGA CTCGAGATTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGT GGCCGAATCC ATTACACTGA GATGTATGAA
    ATGCTGACTC TTATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCTTC CAAAGTGGCC
    TATAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGAG
    CCAGAGGTTA GGGAGTTTCA AAGAGTGCAG CCCTCTAACC GTGGCAGCTA CCTTCCCGTG
    GACACTCAGG ACCACGCTGT CTCTGGGAGG GCCTCCTCCA TGCCTCGTCT CACTGTGGAT
    CCCCAGGTGG TGACGGACAC CAGCTCGATG CGGCGGTCAT TTTCCACCAT CCGGGACAAA
    CGCACCAACA CCTCCTGGCT GGATGAGTTC TCCATGGAGC GCAGCAGCGA CAACACCTAC
    AAGTCCCGCC GGCGCAGCTA CCACTCCTCT CTCCAGCTCT CTGCCCGGCG CCTCAACGCT
    GACTCAGGCC ACAGGTCTGA GGGGCACCGC TCGGGCGGCA GGGAGCGGGG CCGATCCAAA
    GAGCGCAAGC ACTTGCTGTC CCCTGACATC TCCCGCTGCA ACTCAGAGGA GAGGAGTCCC
    CAGGCACACG ATGAGTCCCC AGAGCGGCGG CGCGAGTCCC GGTCACCCAG CGAGGGCAGG
    TCGCAAACGC CCAACCGGCA GGGAACGGGC TCCCTGAGCG AAAGCTCCAT CCCCTCCATC
    TCGGACACCA GCACACCCCG GCGAGGCCGC CGCCAGCTCC CACCTGTGCC CCCAAAACCG
    CGTCCCCTCC TCTCCTACGC CTCCATGCTG CGGCACGCTG CCGGAGACGC CTCACCACCT
    CCCGATGAGA CCGAAGGGGG GTCCCCGCTC CTCTCCAAGG AGCCCAACGC CACCGGCCTG
    ACCGAGTCTT CCAGCTCCCC GGCGGGGAAG CCGAGCCGGC CGTCCACCCC GCAGCGTTAC
    ATCTCGGAGC CCTACCTGGC CCTGCACGAC GACTCGCACG CTTCGGACTG CGGCGAGGAG
    GAGACGCTCA CCTTCGAGGC GGCCGTGGCC ACCAGTCTGG GTCGTTCCAA CACCATCGGC
    TCGGCCCCAC CGCTGCGACA CAGCTGGCAG ATGCCCAACG GGCACTACCG GAGACGGAGG
    CGAGGGGCCG GGCAGGGCGT GATGTGCGGG GCCAGCATCG ATGTGCTCAG CGACACCGAG
    GAAGATGACA AGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145962.2
    CDS127..7404
    Translation

    Target ORF information:

    RefSeq Version XM_015290476.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290476.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATTTTTGTGG CTCTCATCAT CATCACATTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CGGTCATCAT GGACAATTTT
    GAGTACCTGA CTCGAGATTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGT GGCCGAATCC ATTACACTGA GATGTATGAA
    ATGCTGACTC TTATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCTTC CAAAGTGGCC
    TATAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGAG
    CCAGAGGTTA GGGAGTTTCA AAGAGTGCAG CCCTCTAACC GTGGCAGCTA CCTTCCCGTG
    GACACTCAGG ACCACGCTGT CTCTGGGAGG GCCTCCTCCA TGCCTCGTCT CACTGTGGAT
    CCCCAGGTGG TGACGGACAC CAGCTCGATG CGGCGGTCAT TTTCCACCAT CCGGGACAAA
    CGCACCAACA CCTCCTGGCT GGATGAGTTC TCCATGGAGC GCAGCAGCGA CAACACCTAC
    AAGTCCCGCC GGCGCAGCTA CCACTCCTCT CTCCAGCTCT CTGCCCGGCG CCTCAACGCT
    GACTCAGGCC ACAGGTCTGA GGGGCACCGC TCGGGCGGCA GGGAGCGGGG CCGATCCAAA
    GAGCGCAAGC ACTTGCTGTC CCCTGACATC TCCCGCTGCA ACTCAGAGGA GAGGAGTCCC
    CAGGCACACG ATGAGTCCCC AGAGCGGCGG CGCGAGTCCC GGTCACCCAG CGAGGGCAGG
    TCGCAAACGC CCAACCGGCA GGGAACGGGC TCCCTGAGCG AAAGCTCCAT CCCCTCCATC
    TCGGACACCA GCACACCCCG GCGAGGCCGC CGCCAGCTCC CACCTGTGCC CCCAAAACCG
    CGTCCCCTCC TCTCCTACGC CTCCATGCTG CGGCACGCTG CCGGAGACGC CTCACCACCT
    CCCGATGAGA CCGAAGGGGG GTCCCCGCTC CTCTCCAAGG AGCCCAACGC CACCGGCCTG
    ACCGAGTCTT CCAGCTCCCC GGCGGGGAAG CCGAGCCGGC CGTCCACCCC GCAGCGTTAC
    ATCTCGGAGC CCTACCTGGC CCTGCACGAC GACTCGCACG CTTCGGACTG CGGCGAGGAG
    GAGACGCTCA CCTTCGAGGC GGCCGTGGCC ACCAGTCTGG GTCGTTCCAA CACCATCGGC
    TCGGCCCCAC CGCTGCGACA CAGCTGGCAG ATGCCCAACG GGCACTACCG GAGACGGAGG
    CGAGGGGCCG GGCAGGGCGT GATGTGCGGG GCCAGCATCG ATGTGCTCAG CGACACCGAG
    GAAGATGACA AGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152507
    Clone ID Related Accession (Same CDS sequence) XM_015290478.2
    Accession Version XM_015290478.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290478.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATTTTTGTGG CTCTCATCAT CATCACATTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CGGTCATCAT GGACAATTTT
    GAGTACCTGA CTCGAGATTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGC GGCAGGATCT CGTATAAGGA TATGTACAAA
    TTAGTACGGG TGATCTCGCC TCCTCTGGGC CTAGGAGAGA ACTGCCCCTA CAGGGTGGCG
    TGCAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGAG
    CCAGAGGTTA GGGAGTTTCA AAGAGTGCAG CCCTCTAACC GTGGCAGCTA CCTTCCCGTG
    GACACTCAGG ACCACGCTGT CTCTGGGAGG GCCTCCTCCA TGCCTCGTCT CACTGTGGAT
    CCCCAGGTGG TGACGGACAC CAGCTCGATG CGGCGGTCAT TTTCCACCAT CCGGGACAAA
    CGCACCAACA CCTCCTGGCT GGATGAGTTC TCCATGGAGC GCAGCAGCGA CAACACCTAC
    AAGTCCCGCC GGCGCAGCTA CCACTCCTCT CTCCAGCTCT CTGCCCGGCG CCTCAACGCT
    GACTCAGGCC ACAGGTCTGA GGGGCACCGC TCGGGCGGCA GGGAGCGGGG CCGATCCAAA
    GAGCGCAAGC ACTTGCTGTC CCCTGACATC TCCCGCTGCA ACTCAGAGGA GAGGAGTCCC
    CAGGCACACG ATGAGTCCCC AGAGCGGCGG CGCGAGTCCC GGTCACCCAG CGAGGGCAGG
    TCGCAAACGC CCAACCGGCA GGGAACGGGC TCCCTGAGCG AAAGCTCCAT CCCCTCCATC
    TCGGACACCA GCACACCCCG GCGAGGCCGC CGCCAGCTCC CACCTGTGCC CCCAAAACCG
    CGTCCCCTCC TCTCCTACGC CTCCATGCTG CGGCACGCTG CCGGAGACGC CTCACCACCT
    CCCGATGAGA CCGAAGGGGG GTCCCCGCTC CTCTCCAAGG AGCCCAACGC CACCGGCCTG
    ACCGAGTCTT CCAGCTCCCC GGCGGGGAAG CCGAGCCGGC CGTCCACCCC GCAGCGTTAC
    ATCTCGGAGC CCTACCTGGC CCTGCACGAC GACTCGCACG CTTCGGACTG CGGCGAGGAG
    GAGACGCTCA CCTTCGAGGC GGCCGTGGCC ACCAGTCTGG GTCGTTCCAA CACCATCGGC
    TCGGCCCCAC CGCTGCGACA CAGCTGGCAG ATGCCCAACG GGCACTACCG GAGACGGAGG
    CGAGGGGCCG GGCAGGGCGT GATGTGCGGG GCCAGCATCG ATGTGCTCAG CGACACCGAG
    GAAGATGACA AGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145964.2
    CDS128..7405
    Translation

    Target ORF information:

    RefSeq Version XM_015290478.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290478.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATTTTTGTGG CTCTCATCAT CATCACATTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CGGTCATCAT GGACAATTTT
    GAGTACCTGA CTCGAGATTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGC GGCAGGATCT CGTATAAGGA TATGTACAAA
    TTAGTACGGG TGATCTCGCC TCCTCTGGGC CTAGGAGAGA ACTGCCCCTA CAGGGTGGCG
    TGCAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGAG
    CCAGAGGTTA GGGAGTTTCA AAGAGTGCAG CCCTCTAACC GTGGCAGCTA CCTTCCCGTG
    GACACTCAGG ACCACGCTGT CTCTGGGAGG GCCTCCTCCA TGCCTCGTCT CACTGTGGAT
    CCCCAGGTGG TGACGGACAC CAGCTCGATG CGGCGGTCAT TTTCCACCAT CCGGGACAAA
    CGCACCAACA CCTCCTGGCT GGATGAGTTC TCCATGGAGC GCAGCAGCGA CAACACCTAC
    AAGTCCCGCC GGCGCAGCTA CCACTCCTCT CTCCAGCTCT CTGCCCGGCG CCTCAACGCT
    GACTCAGGCC ACAGGTCTGA GGGGCACCGC TCGGGCGGCA GGGAGCGGGG CCGATCCAAA
    GAGCGCAAGC ACTTGCTGTC CCCTGACATC TCCCGCTGCA ACTCAGAGGA GAGGAGTCCC
    CAGGCACACG ATGAGTCCCC AGAGCGGCGG CGCGAGTCCC GGTCACCCAG CGAGGGCAGG
    TCGCAAACGC CCAACCGGCA GGGAACGGGC TCCCTGAGCG AAAGCTCCAT CCCCTCCATC
    TCGGACACCA GCACACCCCG GCGAGGCCGC CGCCAGCTCC CACCTGTGCC CCCAAAACCG
    CGTCCCCTCC TCTCCTACGC CTCCATGCTG CGGCACGCTG CCGGAGACGC CTCACCACCT
    CCCGATGAGA CCGAAGGGGG GTCCCCGCTC CTCTCCAAGG AGCCCAACGC CACCGGCCTG
    ACCGAGTCTT CCAGCTCCCC GGCGGGGAAG CCGAGCCGGC CGTCCACCCC GCAGCGTTAC
    ATCTCGGAGC CCTACCTGGC CCTGCACGAC GACTCGCACG CTTCGGACTG CGGCGAGGAG
    GAGACGCTCA CCTTCGAGGC GGCCGTGGCC ACCAGTCTGG GTCGTTCCAA CACCATCGGC
    TCGGCCCCAC CGCTGCGACA CAGCTGGCAG ATGCCCAACG GGCACTACCG GAGACGGAGG
    CGAGGGGCCG GGCAGGGCGT GATGTGCGGG GCCAGCATCG ATGTGCTCAG CGACACCGAG
    GAAGATGACA AGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152508
    Clone ID Related Accession (Same CDS sequence) XM_015290479.2
    Accession Version XM_015290479.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290479.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGACCAACAA GGGTCGGGAT
    ATCAAGACTA TCAAGTCTCT CCGTGTGCTG CGGGTCTTGA GGCCCCTGAA AACCATCAAG
    CGGCTGCCGA AGCTCAAGGC TGTCTTTGAC TGTGTTGTGA CATCCCTCAA GAACGTCTTC
    AACATCCTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CTGTCATCGC CGTCCAGCTC
    TTCAAGGGGA AGTTTTTCTA CTGCACTGAC AGCTCTAAGG ACACAGAGAA GGACTGCATA
    GGGAACTATG TGGACCATGA GAAGAACAAG ATGGAGGTGA AGTGCCGGGA GTGGAAACGA
    CATGAGTTTC ACTATGACAA TATAATCTGG GCGTTGCTCA CCCTGTTCAC AGTCTCCACC
    GGCGAGGGTT GGCCCCAGGT GCTGCAGCAT TCGGTGGATG TGACGGAGGA GGACCGTGGG
    CCCAGCCGCA GTAACCGCAT GGAGATGTCC ATTTTTTACG TTGTCTATTT TGTGGTCTTC
    CCTTTCTTCT TCGTCAACAT TTTTGTGGCT CTCATCATCA TCACATTCCA AGAGCAAGGA
    GACAAAATGA TGGAGGAGTG CAGTCTGGAG AAGAACGAGA GGGCCTGTAT TGACTTTGCC
    ATCAGTGCCA AGCCCCTCAC ACGCTACATG CCCCAGAACC GGCACACCTT CCAGTATCGG
    GTCTGGCACT TTGTGGTCTC TCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCTTTG
    AACACTGTGG TGCTGATGAT GAAGTACTAC TCTGCTCCAT ACACCTATGA GCTGGCCCTG
    AAGTACCTGA ACATCGCTTT CACCATGGTG TTCTCTTTGG AGTGCGTCCT TAAGATCATT
    GCTTTTGGCT TCCTGAATTA TTTCCGGGAT ACCTGGAATA TCTTTGACTT CATCACCGTC
    ATCGGCAGCA TCACAGAGAT CATCCTGACA GACACCAAGC TGGTGAACAC CAGTAGCTTC
    AACATGAGTT TCCTGAAACT CTTCCGGGCG GCTCGGCTCA TCAAGCTGCT GCGCCAGGGT
    TACACCATCC GCATCCTGCT GTGGACGTTC GTGCAGTCCT TCAAGGCTCT CCCCTATGTC
    TGCCTCCTAA TCGCGATGCT TTTCTTCATC TATGCCATCA TTGGGATGCA GGTTTTCGGC
    AACATCAAAC TGGATGAGGA AAGCCACATT AACCGGCACA ACAACTTCCG CAGCTTCCTG
    GGCTCCCTCA TGCTCCTCTT CAGGAGTGCC ACCGGTGAGG CATGGCAGGA GATCATGCTC
    TCCTGCCTGG AGGGCAAGGG CTGCGAGCCG GACACGACAG CGACGTCCGG GCAGAATGAG
    AACGAGCGCT GCGGCACCGA CCTGGCCTAC GTCTACTTTG TCTCCTTCAT CTTCTTCTGC
    TCATTCCTGA TGCTCAACCT GTTCGTGGCG GTCATCATGG ACAATTTTGA GTACCTGACT
    CGAGATTCCT CCATCCTGGG ACCTCACCAT CTAGATGAAT TTGTCCGGAT CTGGGCAGAG
    TATGACAGAG CAGCGTGTGG CCGAATCCAT TACACTGAGA TGTATGAAAT GCTGACTCTT
    ATGTCACCAC CGCTAGGCCT CGGCAAGAGA TGTCCTTCCA AAGTGGCCTA TAAGAGACTG
    GTGCTGATGA ACATGCCCGT GGCCGAGGAC ATGACGGTCC ATTTCACCTC CACCCTGATG
    GCGCTGATAC GCACCGCCCT GGACATCAAA ATTGCCAAAG GTGGTGCAGA TTGGCAGCAG
    TTAGACTCTG AGCTTCAAAA GGAGATCCTG ACCATTTGGC CTCACCTGTC CCAGAAGATG
    CTTGACCTGT TGGTACCCAT GCCAAAAACC TCTGACCTGA CTGTTGGCAA AATATATGCA
    GCCATGATGA TCATGGATTA CTACAAGCAG AGCAAGGCGA AGAAGCAGAG GCAGCAGCTG
    GAGGAGCAGA AAAATGCGCC CATGTTCCAG CGCATGGAGC CATCCTCTTT GCCACAGGAA
    ATCATCTCCA ACGCAAAGGC TCTGCCTTAC CTCCAGCAGG AGACCCTCAC AGGGCTGAGC
    AGCCGGAGCG GGTTCCCCTC GCTGAGCCCG CTCTCACCAC AGGAGATCTT CCAGCTGGCG
    TGCATGGATC CGGCCCACGG GCAGTTCCAG GAGCACCAGT CTCTGGAGCC AGAGGTTAGG
    GAGTTTCAAA GAGTGCAGCC CTCTAACCGT GGCAGCTACC TTCCCGTGGA CACTCAGGAC
    CACGCTGTCT CTGGGAGGGC CTCCTCCATG CCTCGTCTCA CTGTGGATCC CCAGGTGGTG
    ACGGACACCA GCTCGATGCG GCGGTCATTT TCCACCATCC GGGACAAACG CACCAACACC
    TCCTGGCTGG ATGAGTTCTC CATGGAGCGC AGCAGCGACA ACACCTACAA GTCCCGCCGG
    CGCAGCTACC ACTCCTCTCT CCAGCTCTCT GCCCGGCGCC TCAACGCTGA CTCAGGCCAC
    AGGTCTGAGG GGCACCGCTC GGGCGGCAGG GAGCGGGGCC GATCCAAAGA GCGCAAGCAC
    TTGCTGTCCC CTGACATCTC CCGCTGCAAC TCAGAGGAGA GGAGTCCCCA GGCACACGAT
    GAGTCCCCAG AGCGGCGGCG CGAGTCCCGG TCACCCAGCG AGGGCAGGTC GCAAACGCCC
    AACCGGCAGG GAACGGGCTC CCTGAGCGAA AGCTCCATCC CCTCCATCTC GGACACCAGC
    ACACCCCGGC GAGGCCGCCG CCAGCTCCCA CCTGTGCCCC CAAAACCGCG TCCCCTCCTC
    TCCTACGCCT CCATGCTGCG GCACGCTGCC GGAGACGCCT CACCACCTCC CGATGAGACC
    GAAGGGGGGT CCCCGCTCCT CTCCAAGGAG CCCAACGCCA CCGGCCTGAC CGAGTCTTCC
    AGCTCCCCGG CGGGGAAGCC GAGCCGGCCG TCCACCCCGC AGCGTTACAT CTCGGAGCCC
    TACCTGGCCC TGCACGACGA CTCGCACGCT TCGGACTGCG GCGAGGAGGA GACGCTCACC
    TTCGAGGCGG CCGTGGCCAC CAGTCTGGGT CGTTCCAACA CCATCGGCTC GGCCCCACCG
    CTGCGACACA GCTGGCAGAT GCCCAACGGG CACTACCGGA GACGGAGGCG AGGGGCCGGG
    CAGGGCGTGA TGTGCGGGGC CAGCATCGAT GTGCTCAGCG ACACCGAGGA AGATGACAAG
    TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145965.2
    CDS128..7393
    Translation

    Target ORF information:

    RefSeq Version XM_015290479.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290479.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGACCAACAA GGGTCGGGAT
    ATCAAGACTA TCAAGTCTCT CCGTGTGCTG CGGGTCTTGA GGCCCCTGAA AACCATCAAG
    CGGCTGCCGA AGCTCAAGGC TGTCTTTGAC TGTGTTGTGA CATCCCTCAA GAACGTCTTC
    AACATCCTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CTGTCATCGC CGTCCAGCTC
    TTCAAGGGGA AGTTTTTCTA CTGCACTGAC AGCTCTAAGG ACACAGAGAA GGACTGCATA
    GGGAACTATG TGGACCATGA GAAGAACAAG ATGGAGGTGA AGTGCCGGGA GTGGAAACGA
    CATGAGTTTC ACTATGACAA TATAATCTGG GCGTTGCTCA CCCTGTTCAC AGTCTCCACC
    GGCGAGGGTT GGCCCCAGGT GCTGCAGCAT TCGGTGGATG TGACGGAGGA GGACCGTGGG
    CCCAGCCGCA GTAACCGCAT GGAGATGTCC ATTTTTTACG TTGTCTATTT TGTGGTCTTC
    CCTTTCTTCT TCGTCAACAT TTTTGTGGCT CTCATCATCA TCACATTCCA AGAGCAAGGA
    GACAAAATGA TGGAGGAGTG CAGTCTGGAG AAGAACGAGA GGGCCTGTAT TGACTTTGCC
    ATCAGTGCCA AGCCCCTCAC ACGCTACATG CCCCAGAACC GGCACACCTT CCAGTATCGG
    GTCTGGCACT TTGTGGTCTC TCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCTTTG
    AACACTGTGG TGCTGATGAT GAAGTACTAC TCTGCTCCAT ACACCTATGA GCTGGCCCTG
    AAGTACCTGA ACATCGCTTT CACCATGGTG TTCTCTTTGG AGTGCGTCCT TAAGATCATT
    GCTTTTGGCT TCCTGAATTA TTTCCGGGAT ACCTGGAATA TCTTTGACTT CATCACCGTC
    ATCGGCAGCA TCACAGAGAT CATCCTGACA GACACCAAGC TGGTGAACAC CAGTAGCTTC
    AACATGAGTT TCCTGAAACT CTTCCGGGCG GCTCGGCTCA TCAAGCTGCT GCGCCAGGGT
    TACACCATCC GCATCCTGCT GTGGACGTTC GTGCAGTCCT TCAAGGCTCT CCCCTATGTC
    TGCCTCCTAA TCGCGATGCT TTTCTTCATC TATGCCATCA TTGGGATGCA GGTTTTCGGC
    AACATCAAAC TGGATGAGGA AAGCCACATT AACCGGCACA ACAACTTCCG CAGCTTCCTG
    GGCTCCCTCA TGCTCCTCTT CAGGAGTGCC ACCGGTGAGG CATGGCAGGA GATCATGCTC
    TCCTGCCTGG AGGGCAAGGG CTGCGAGCCG GACACGACAG CGACGTCCGG GCAGAATGAG
    AACGAGCGCT GCGGCACCGA CCTGGCCTAC GTCTACTTTG TCTCCTTCAT CTTCTTCTGC
    TCATTCCTGA TGCTCAACCT GTTCGTGGCG GTCATCATGG ACAATTTTGA GTACCTGACT
    CGAGATTCCT CCATCCTGGG ACCTCACCAT CTAGATGAAT TTGTCCGGAT CTGGGCAGAG
    TATGACAGAG CAGCGTGTGG CCGAATCCAT TACACTGAGA TGTATGAAAT GCTGACTCTT
    ATGTCACCAC CGCTAGGCCT CGGCAAGAGA TGTCCTTCCA AAGTGGCCTA TAAGAGACTG
    GTGCTGATGA ACATGCCCGT GGCCGAGGAC ATGACGGTCC ATTTCACCTC CACCCTGATG
    GCGCTGATAC GCACCGCCCT GGACATCAAA ATTGCCAAAG GTGGTGCAGA TTGGCAGCAG
    TTAGACTCTG AGCTTCAAAA GGAGATCCTG ACCATTTGGC CTCACCTGTC CCAGAAGATG
    CTTGACCTGT TGGTACCCAT GCCAAAAACC TCTGACCTGA CTGTTGGCAA AATATATGCA
    GCCATGATGA TCATGGATTA CTACAAGCAG AGCAAGGCGA AGAAGCAGAG GCAGCAGCTG
    GAGGAGCAGA AAAATGCGCC CATGTTCCAG CGCATGGAGC CATCCTCTTT GCCACAGGAA
    ATCATCTCCA ACGCAAAGGC TCTGCCTTAC CTCCAGCAGG AGACCCTCAC AGGGCTGAGC
    AGCCGGAGCG GGTTCCCCTC GCTGAGCCCG CTCTCACCAC AGGAGATCTT CCAGCTGGCG
    TGCATGGATC CGGCCCACGG GCAGTTCCAG GAGCACCAGT CTCTGGAGCC AGAGGTTAGG
    GAGTTTCAAA GAGTGCAGCC CTCTAACCGT GGCAGCTACC TTCCCGTGGA CACTCAGGAC
    CACGCTGTCT CTGGGAGGGC CTCCTCCATG CCTCGTCTCA CTGTGGATCC CCAGGTGGTG
    ACGGACACCA GCTCGATGCG GCGGTCATTT TCCACCATCC GGGACAAACG CACCAACACC
    TCCTGGCTGG ATGAGTTCTC CATGGAGCGC AGCAGCGACA ACACCTACAA GTCCCGCCGG
    CGCAGCTACC ACTCCTCTCT CCAGCTCTCT GCCCGGCGCC TCAACGCTGA CTCAGGCCAC
    AGGTCTGAGG GGCACCGCTC GGGCGGCAGG GAGCGGGGCC GATCCAAAGA GCGCAAGCAC
    TTGCTGTCCC CTGACATCTC CCGCTGCAAC TCAGAGGAGA GGAGTCCCCA GGCACACGAT
    GAGTCCCCAG AGCGGCGGCG CGAGTCCCGG TCACCCAGCG AGGGCAGGTC GCAAACGCCC
    AACCGGCAGG GAACGGGCTC CCTGAGCGAA AGCTCCATCC CCTCCATCTC GGACACCAGC
    ACACCCCGGC GAGGCCGCCG CCAGCTCCCA CCTGTGCCCC CAAAACCGCG TCCCCTCCTC
    TCCTACGCCT CCATGCTGCG GCACGCTGCC GGAGACGCCT CACCACCTCC CGATGAGACC
    GAAGGGGGGT CCCCGCTCCT CTCCAAGGAG CCCAACGCCA CCGGCCTGAC CGAGTCTTCC
    AGCTCCCCGG CGGGGAAGCC GAGCCGGCCG TCCACCCCGC AGCGTTACAT CTCGGAGCCC
    TACCTGGCCC TGCACGACGA CTCGCACGCT TCGGACTGCG GCGAGGAGGA GACGCTCACC
    TTCGAGGCGG CCGTGGCCAC CAGTCTGGGT CGTTCCAACA CCATCGGCTC GGCCCCACCG
    CTGCGACACA GCTGGCAGAT GCCCAACGGG CACTACCGGA GACGGAGGCG AGGGGCCGGG
    CAGGGCGTGA TGTGCGGGGC CAGCATCGAT GTGCTCAGCG ACACCGAGGA AGATGACAAG
    TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152509
    Clone ID Related Accession (Same CDS sequence) XM_015290480.2
    Accession Version XM_015290480.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGACCAACAA GGGTCGGGAT
    ATCAAGACTA TCAAGTCTCT CCGTGTGCTG CGGGTCTTGA GGCCCCTGAA AACCATCAAG
    CGGCTGCCGA AGCTCAAGGC TGTCTTTGAC TGTGTTGTGA CATCCCTCAA GAACGTCTTC
    AACATCCTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CTGTCATCGC CGTCCAGCTC
    TTCAAGGGGA AGTTTTTCTA CTGCACTGAC AGCTCTAAGG ACACAGAGAA GGACTGCATA
    GGGAACTATG TGGACCATGA GAAGAACAAG ATGGAGGTGA AGTGCCGGGA GTGGAAACGA
    CATGAGTTTC ACTATGACAA TATAATCTGG GCGTTGCTCA CCCTGTTCAC AGTCTCCACC
    GGCGAGGGTT GGCCCCAGGT GCTGCAGCAT TCGGTGGATG TGACGGAGGA GGACCGTGGG
    CCCAGCCGCA GTAACCGCAT GGAGATGTCC ATTTTTTACG TTGTCTATTT TGTGGTCTTC
    CCTTTCTTCT TCGTCAACAT TTTTGTGGCT CTCATCATCA TCACATTCCA AGAGCAAGGA
    GACAAAATGA TGGAGGAGTG CAGTCTGGAG AAGAACGAGA GGGCCTGTAT TGACTTTGCC
    ATCAGTGCCA AGCCCCTCAC ACGCTACATG CCCCAGAACC GGCACACCTT CCAGTATCGG
    GTCTGGCACT TTGTGGTCTC TCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCTTTG
    AACACTGTGG TGCTGATGAT GAAGTACTAC TCTGCTCCAT ACACCTATGA GCTGGCCCTG
    AAGTACCTGA ACATCGCTTT CACCATGGTG TTCTCTTTGG AGTGCGTCCT TAAGATCATT
    GCTTTTGGCT TCCTGAATTA TTTCCGGGAT ACCTGGAATA TCTTTGACTT CATCACCGTC
    ATCGGCAGCA TCACAGAGAT CATCCTGACA GACACCAAGC TGGTGAACAC CAGTAGCTTC
    AACATGAGTT TCCTGAAACT CTTCCGGGCG GCTCGGCTCA TCAAGCTGCT GCGCCAGGGT
    TACACCATCC GCATCCTGCT GTGGACGTTC GTGCAGTCCT TCAAGGCTCT CCCCTATGTC
    TGCCTCCTAA TCGCGATGCT TTTCTTCATC TATGCCATCA TTGGGATGCA GGTTTTCGGC
    AACATCAAAC TGGATGAGGA AAGCCACATT AACCGGCACA ACAACTTCCG CAGCTTCCTG
    GGCTCCCTCA TGCTCCTCTT CAGGAGTGCC ACCGGTGAGG CATGGCAGGA GATCATGCTC
    TCCTGCCTGG AGGGCAAGGG CTGCGAGCCG GACACGACAG CGACGTCCGG GCAGAATGAG
    AACGAGCGCT GCGGCACCGA CCTGGCCTAC GTCTACTTTG TCTCCTTCAT CTTCTTCTGC
    TCATTCCTGA TGCTCAACCT GTTCGTGGCG GTCATCATGG ACAATTTTGA GTACCTGACT
    CGAGATTCCT CCATCCTGGG ACCTCACCAT CTAGATGAAT TTGTCCGGAT CTGGGCAGAG
    TATGACAGAG CAGCGTGCGG CAGGATCTCG TATAAGGATA TGTACAAATT AGTACGGGTG
    ATCTCGCCTC CTCTGGGCCT AGGAGAGAAC TGCCCCTACA GGGTGGCGTG CAAGAGACTG
    GTGCTGATGA ACATGCCCGT GGCCGAGGAC ATGACGGTCC ATTTCACCTC CACCCTGATG
    GCGCTGATAC GCACCGCCCT GGACATCAAA ATTGCCAAAG GTGGTGCAGA TTGGCAGCAG
    TTAGACTCTG AGCTTCAAAA GGAGATCCTG ACCATTTGGC CTCACCTGTC CCAGAAGATG
    CTTGACCTGT TGGTACCCAT GCCAAAAACC TCTGACCTGA CTGTTGGCAA AATATATGCA
    GCCATGATGA TCATGGATTA CTACAAGCAG AGCAAGGCGA AGAAGCAGAG GCAGCAGCTG
    GAGGAGCAGA AAAATGCGCC CATGTTCCAG CGCATGGAGC CATCCTCTTT GCCACAGGAA
    ATCATCTCCA ACGCAAAGGC TCTGCCTTAC CTCCAGCAGG AGACCCTCAC AGGGCTGAGC
    AGCCGGAGCG GGTTCCCCTC GCTGAGCCCG CTCTCACCAC AGGAGATCTT CCAGCTGGCG
    TGCATGGATC CGGCCCACGG GCAGTTCCAG GAGCACCAGT CTCTGGAGCC AGAGGTTAGG
    GAGTTTCAAA GAGTGCAGCC CTCTAACCGT GGCAGCTACC TTCCCGTGGA CACTCAGGAC
    CACGCTGTCT CTGGGAGGGC CTCCTCCATG CCTCGTCTCA CTGTGGATCC CCAGGTGGTG
    ACGGACACCA GCTCGATGCG GCGGTCATTT TCCACCATCC GGGACAAACG CACCAACACC
    TCCTGGCTGG ATGAGTTCTC CATGGAGCGC AGCAGCGACA ACACCTACAA GTCCCGCCGG
    CGCAGCTACC ACTCCTCTCT CCAGCTCTCT GCCCGGCGCC TCAACGCTGA CTCAGGCCAC
    AGGTCTGAGG GGCACCGCTC GGGCGGCAGG GAGCGGGGCC GATCCAAAGA GCGCAAGCAC
    TTGCTGTCCC CTGACATCTC CCGCTGCAAC TCAGAGGAGA GGAGTCCCCA GGCACACGAT
    GAGTCCCCAG AGCGGCGGCG CGAGTCCCGG TCACCCAGCG AGGGCAGGTC GCAAACGCCC
    AACCGGCAGG GAACGGGCTC CCTGAGCGAA AGCTCCATCC CCTCCATCTC GGACACCAGC
    ACACCCCGGC GAGGCCGCCG CCAGCTCCCA CCTGTGCCCC CAAAACCGCG TCCCCTCCTC
    TCCTACGCCT CCATGCTGCG GCACGCTGCC GGAGACGCCT CACCACCTCC CGATGAGACC
    GAAGGGGGGT CCCCGCTCCT CTCCAAGGAG CCCAACGCCA CCGGCCTGAC CGAGTCTTCC
    AGCTCCCCGG CGGGGAAGCC GAGCCGGCCG TCCACCCCGC AGCGTTACAT CTCGGAGCCC
    TACCTGGCCC TGCACGACGA CTCGCACGCT TCGGACTGCG GCGAGGAGGA GACGCTCACC
    TTCGAGGCGG CCGTGGCCAC CAGTCTGGGT CGTTCCAACA CCATCGGCTC GGCCCCACCG
    CTGCGACACA GCTGGCAGAT GCCCAACGGG CACTACCGGA GACGGAGGCG AGGGGCCGGG
    CAGGGCGTGA TGTGCGGGGC CAGCATCGAT GTGCTCAGCG ACACCGAGGA AGATGACAAG
    TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145966.2
    CDS128..7393
    Translation

    Target ORF information:

    RefSeq Version XM_015290480.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290480.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGACCAACAA GGGTCGGGAT
    ATCAAGACTA TCAAGTCTCT CCGTGTGCTG CGGGTCTTGA GGCCCCTGAA AACCATCAAG
    CGGCTGCCGA AGCTCAAGGC TGTCTTTGAC TGTGTTGTGA CATCCCTCAA GAACGTCTTC
    AACATCCTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CTGTCATCGC CGTCCAGCTC
    TTCAAGGGGA AGTTTTTCTA CTGCACTGAC AGCTCTAAGG ACACAGAGAA GGACTGCATA
    GGGAACTATG TGGACCATGA GAAGAACAAG ATGGAGGTGA AGTGCCGGGA GTGGAAACGA
    CATGAGTTTC ACTATGACAA TATAATCTGG GCGTTGCTCA CCCTGTTCAC AGTCTCCACC
    GGCGAGGGTT GGCCCCAGGT GCTGCAGCAT TCGGTGGATG TGACGGAGGA GGACCGTGGG
    CCCAGCCGCA GTAACCGCAT GGAGATGTCC ATTTTTTACG TTGTCTATTT TGTGGTCTTC
    CCTTTCTTCT TCGTCAACAT TTTTGTGGCT CTCATCATCA TCACATTCCA AGAGCAAGGA
    GACAAAATGA TGGAGGAGTG CAGTCTGGAG AAGAACGAGA GGGCCTGTAT TGACTTTGCC
    ATCAGTGCCA AGCCCCTCAC ACGCTACATG CCCCAGAACC GGCACACCTT CCAGTATCGG
    GTCTGGCACT TTGTGGTCTC TCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCTTTG
    AACACTGTGG TGCTGATGAT GAAGTACTAC TCTGCTCCAT ACACCTATGA GCTGGCCCTG
    AAGTACCTGA ACATCGCTTT CACCATGGTG TTCTCTTTGG AGTGCGTCCT TAAGATCATT
    GCTTTTGGCT TCCTGAATTA TTTCCGGGAT ACCTGGAATA TCTTTGACTT CATCACCGTC
    ATCGGCAGCA TCACAGAGAT CATCCTGACA GACACCAAGC TGGTGAACAC CAGTAGCTTC
    AACATGAGTT TCCTGAAACT CTTCCGGGCG GCTCGGCTCA TCAAGCTGCT GCGCCAGGGT
    TACACCATCC GCATCCTGCT GTGGACGTTC GTGCAGTCCT TCAAGGCTCT CCCCTATGTC
    TGCCTCCTAA TCGCGATGCT TTTCTTCATC TATGCCATCA TTGGGATGCA GGTTTTCGGC
    AACATCAAAC TGGATGAGGA AAGCCACATT AACCGGCACA ACAACTTCCG CAGCTTCCTG
    GGCTCCCTCA TGCTCCTCTT CAGGAGTGCC ACCGGTGAGG CATGGCAGGA GATCATGCTC
    TCCTGCCTGG AGGGCAAGGG CTGCGAGCCG GACACGACAG CGACGTCCGG GCAGAATGAG
    AACGAGCGCT GCGGCACCGA CCTGGCCTAC GTCTACTTTG TCTCCTTCAT CTTCTTCTGC
    TCATTCCTGA TGCTCAACCT GTTCGTGGCG GTCATCATGG ACAATTTTGA GTACCTGACT
    CGAGATTCCT CCATCCTGGG ACCTCACCAT CTAGATGAAT TTGTCCGGAT CTGGGCAGAG
    TATGACAGAG CAGCGTGCGG CAGGATCTCG TATAAGGATA TGTACAAATT AGTACGGGTG
    ATCTCGCCTC CTCTGGGCCT AGGAGAGAAC TGCCCCTACA GGGTGGCGTG CAAGAGACTG
    GTGCTGATGA ACATGCCCGT GGCCGAGGAC ATGACGGTCC ATTTCACCTC CACCCTGATG
    GCGCTGATAC GCACCGCCCT GGACATCAAA ATTGCCAAAG GTGGTGCAGA TTGGCAGCAG
    TTAGACTCTG AGCTTCAAAA GGAGATCCTG ACCATTTGGC CTCACCTGTC CCAGAAGATG
    CTTGACCTGT TGGTACCCAT GCCAAAAACC TCTGACCTGA CTGTTGGCAA AATATATGCA
    GCCATGATGA TCATGGATTA CTACAAGCAG AGCAAGGCGA AGAAGCAGAG GCAGCAGCTG
    GAGGAGCAGA AAAATGCGCC CATGTTCCAG CGCATGGAGC CATCCTCTTT GCCACAGGAA
    ATCATCTCCA ACGCAAAGGC TCTGCCTTAC CTCCAGCAGG AGACCCTCAC AGGGCTGAGC
    AGCCGGAGCG GGTTCCCCTC GCTGAGCCCG CTCTCACCAC AGGAGATCTT CCAGCTGGCG
    TGCATGGATC CGGCCCACGG GCAGTTCCAG GAGCACCAGT CTCTGGAGCC AGAGGTTAGG
    GAGTTTCAAA GAGTGCAGCC CTCTAACCGT GGCAGCTACC TTCCCGTGGA CACTCAGGAC
    CACGCTGTCT CTGGGAGGGC CTCCTCCATG CCTCGTCTCA CTGTGGATCC CCAGGTGGTG
    ACGGACACCA GCTCGATGCG GCGGTCATTT TCCACCATCC GGGACAAACG CACCAACACC
    TCCTGGCTGG ATGAGTTCTC CATGGAGCGC AGCAGCGACA ACACCTACAA GTCCCGCCGG
    CGCAGCTACC ACTCCTCTCT CCAGCTCTCT GCCCGGCGCC TCAACGCTGA CTCAGGCCAC
    AGGTCTGAGG GGCACCGCTC GGGCGGCAGG GAGCGGGGCC GATCCAAAGA GCGCAAGCAC
    TTGCTGTCCC CTGACATCTC CCGCTGCAAC TCAGAGGAGA GGAGTCCCCA GGCACACGAT
    GAGTCCCCAG AGCGGCGGCG CGAGTCCCGG TCACCCAGCG AGGGCAGGTC GCAAACGCCC
    AACCGGCAGG GAACGGGCTC CCTGAGCGAA AGCTCCATCC CCTCCATCTC GGACACCAGC
    ACACCCCGGC GAGGCCGCCG CCAGCTCCCA CCTGTGCCCC CAAAACCGCG TCCCCTCCTC
    TCCTACGCCT CCATGCTGCG GCACGCTGCC GGAGACGCCT CACCACCTCC CGATGAGACC
    GAAGGGGGGT CCCCGCTCCT CTCCAAGGAG CCCAACGCCA CCGGCCTGAC CGAGTCTTCC
    AGCTCCCCGG CGGGGAAGCC GAGCCGGCCG TCCACCCCGC AGCGTTACAT CTCGGAGCCC
    TACCTGGCCC TGCACGACGA CTCGCACGCT TCGGACTGCG GCGAGGAGGA GACGCTCACC
    TTCGAGGCGG CCGTGGCCAC CAGTCTGGGT CGTTCCAACA CCATCGGCTC GGCCCCACCG
    CTGCGACACA GCTGGCAGAT GCCCAACGGG CACTACCGGA GACGGAGGCG AGGGGCCGGG
    CAGGGCGTGA TGTGCGGGGC CAGCATCGAT GTGCTCAGCG ACACCGAGGA AGATGACAAG
    TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152510
    Clone ID Related Accession (Same CDS sequence) XM_015290481.2
    Accession Version XM_015290481.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAGC GGGAGCGGCG CCGGAGGCAC
    CACATGTCGG TGTGGGAGCA GCGCACCAGC CAGCTGCGCC GGCACATGCA GATGTCCAGC
    CAGGAGGGCA TCAATAAGGA TGAGCCACCC CTCATCAACC CACATGCCAG CATCTTCCGC
    CGCAAGAAGC CGGGGGACGG TGTTGCCTTG GAGAAGTGCG ATGAAGAGCA AGGCGGCAAG
    GGTGAGCGGC CGCCAGCTGA GGGTCCCGAG CAGCCGGCCC CAGGGACCAA CCCAGGCGGT
    GGGGAGGACA GGAAGAGCCC ATCGCCCCGT GCCAGGAGGG ACAAGGAGAT GTGGCAACAC
    AAAGCGTGCC ACGGGAACTG CGAGCCGGGT GAGGAAGGGG CAGGAGGTGG CATCGAGGAG
    AGGGCGCGGA TGAGACAGAG CCAGCGGCGC AGCCGGCATC GGAAAGCCAG GATGGAGGGG
    AAGGAGACGA CCGGAGCCCT GGAGAGCCGT GCGGGCAGCC AGGAGATGGG GCTGGAGGAG
    CCCTGCCCCG CAGAGGGGAT GCAGGATGGG GAGCGGCGTG GGGATTCTGC TGCCGCGCTG
    GACCTGATTC AGGGCGAGCT GGAGGCGAGC GGGGACCCCA CCAGGACTAA TGGGGTTCCC
    GCCGAGGAGG GAAGCCTCCC AGCATCAGCC CCTGAGCCTT CCCGGGGGAT GGAGGGCAGC
    CTGGGTGAGC AGGACTGCAG CAGCCCAGAC ACCAGTGAGC AGGCCCTGCT GGGGGGGGCG
    GCACTGGGGG CCAGCCGCAC CGTCAGCCAC AGCGAGCCTG ACCTGTCCTC CGTCACCGCC
    AACACTGAGA AGGCCACCGA GAGCACCACC ATCATGATTG ATGTCCAAGA CTCCACTGTG
    GTACAGATTA GCAACAAGAC TGATGGAGAA GCCAGCCCCT TGAAGGAGGC AGAGAGCAAA
    GAGGATGAGG AAGAGATGGA GAAGAAGAAG CGCAAGAAGG AGAAGAGCGA GACGGGCAAA
    GCGATGGTGC CACACAGCTC CATGTTCATC TTCAGCACCA CAAACCCGGT GCGCAGAGCC
    TGCCACTACA TTGTGAACCT GCGCTACTTT GAGATGTGCA TCCTCCTGGT CATCGCCGCC
    AGCAGCATCG CCCTCGCGGC CGAGGATCCC GTCCTCACCA ACTCCGACCG TAACAAAGTG
    CTGAGGTATT TTGATTACGT TTTCACCGGT GTCTTCACCT TCGAGATGGT TATCAAGATG
    ATCGACCAGG GCTTGATCCT GCAGGATGGC TCCTACTTCC GAGACCTCTG GAACATCCTG
    GATTTCATCG TGGTGGTGGG AGCTCTGGTG GCCTTTGCCT TGGCGAATGC TTTGGGGACC
    AACAAGGGTC GGGATATCAA GACTATCAAG TCTCTCCGTG TGCTGCGGGT CTTGAGGCCC
    CTGAAAACCA TCAAGCGGCT GCCGAAGCTC AAGGCTGTCT TTGACTGTGT TGTGACATCC
    CTCAAGAACG TCTTCAACAT CCTCATTGTC TACAAGCTCT TCATGTTCAT CTTTGCTGTC
    ATCGCCGTCC AGCTCTTCAA GGGGAAGTTT TTCTACTGCA CTGACAGCTC TAAGGACACA
    GAGAAGGACT GCATAGGGAA CTATGTGGAC CATGAGAAGA ACAAGATGGA GGTGAAGTGC
    CGGGAGTGGA AACGACATGA GTTTCACTAT GACAATATAA TCTGGGCGTT GCTCACCCTG
    TTCACAGTCT CCACCGGCGA GGGTTGGCCC CAGGTGCTGC AGCATTCGGT GGATGTGACG
    GAGGAGGACC GTGGGCCCAG CCGCAGTAAC CGCATGGAGA TGTCCATTTT TTACGTTGTC
    TATTTTGTGG TCTTCCCTTT CTTCTTCGTC AACATTTTTG TGGCTCTCAT CATCATCACA
    TTCCAAGAGC AAGGAGACAA AATGATGGAG GAGTGCAGTC TGGAGAAGAA CGAGAGGGCC
    TGTATTGACT TTGCCATCAG TGCCAAGCCC CTCACACGCT ACATGCCCCA GAACCGGCAC
    ACCTTCCAGT ATCGGGTCTG GCACTTTGTG GTCTCTCCAT CCTTTGAGTA TACCATCATG
    GCCATGATTG CTTTGAACAC TGTGGTGCTG ATGATGAAGT ACTACTCTGC TCCATACACC
    TATGAGCTGG CCCTGAAGTA CCTGAACATC GCTTTCACCA TGGTGTTCTC TTTGGAGTGC
    GTCCTTAAGA TCATTGCTTT TGGCTTCCTG AATTATTTCC GGGATACCTG GAATATCTTT
    GACTTCATCA CCGTCATCGG CAGCATCACA GAGATCATCC TGACAGACAC CAAGCTGGTG
    AACACCAGTA GCTTCAACAT GAGTTTCCTG AAACTCTTCC GGGCGGCTCG GCTCATCAAG
    CTGCTGCGCC AGGGTTACAC CATCCGCATC CTGCTGTGGA CGTTCGTGCA GTCCTTCAAG
    GCTCTCCCCT ATGTCTGCCT CCTAATCGCG ATGCTTTTCT TCATCTATGC CATCATTGGG
    ATGCAGGTTT TCGGCAACAT CAAACTGGAT GAGGAAAGCC ACATTAACCG GCACAACAAC
    TTCCGCAGCT TCCTGGGCTC CCTCATGCTC CTCTTCAGGA GTGCCACCGG TGAGGCATGG
    CAGGAGATCA TGCTCTCCTG CCTGGAGGGC AAGGGCTGCG AGCCGGACAC GACAGCGACG
    TCCGGGCAGA ATGAGAACGA GCGCTGCGGC ACCGACCTGG CCTACGTCTA CTTTGTCTCC
    TTCATCTTCT TCTGCTCATT CCTGATGCTC AACCTGTTCG TGGCGGTCAT CATGGACAAT
    TTTGAGTACC TGACTCGAGA TTCCTCCATC CTGGGACCTC ACCATCTAGA TGAATTTGTC
    CGGATCTGGG CAGAGTATGA CAGAGCAGCG TGTGGCCGAA TCCATTACAC TGAGATGTAT
    GAAATGCTGA CTCTTATGTC ACCACCGCTA GGCCTCGGCA AGAGATGTCC TTCCAAAGTG
    GCCTATAAGA GACTGGTGCT GATGAACATG CCCGTGGCCG AGGACATGAC GGTCCATTTC
    ACCTCCACCC TGATGGCGCT GATACGCACC GCCCTGGACA TCAAAATTGC CAAAGGTGGT
    GCAGATTGGC AGCAGTTAGA CTCTGAGCTT CAAAAGGAGA TCCTGACCAT TTGGCCTCAC
    CTGTCCCAGA AGATGCTTGA CCTGTTGGTA CCCATGCCAA AAACCTCTGA CCTGACTGTT
    GGCAAAATAT ATGCAGCCAT GATGATCATG GATTACTACA AGCAGAGCAA GGCGAAGAAG
    CAGAGGCAGC AGCTGGAGGA GCAGAAAAAT GCGCCCATGT TCCAGCGCAT GGAGCCATCC
    TCTTTGCCAC AGGAAATCAT CTCCAACGCA AAGGCTCTGC CTTACCTCCA GCAGGAGACC
    CTCACAGGGC TGAGCAGCCG GAGCGGGTTC CCCTCGCTGA GCCCGCTCTC ACCACAGGAG
    ATCTTCCAGC TGGCGTGCAT GGATCCGGCC CACGGGCAGT TCCAGGAGCA CCAGTCTCTG
    GAGCCAGAGG TTAGGGAGTT TCAAAGAGTG CAGCCCTCTA ACCGTGGCAG CTACCTTCCC
    GTGGACACTC AGGACCACGC TGTCTCTGGG AGGGCCTCCT CCATGCCTCG TCTCACTGTG
    GATCCCCAGG TGGTGACGGA CACCAGCTCG ATGCGGCGGT CATTTTCCAC CATCCGGGAC
    AAACGCACCA ACACCTCCTG GCTGGATGAG TTCTCCATGG AGCGCAGCAG CGACAACACC
    TACAAGTCCC GCCGGCGCAG CTACCACTCC TCTCTCCAGC TCTCTGCCCG GCGCCTCAAC
    GCTGACTCAG GCCACAGGTC TGAGGGGCAC CGCTCGGGCG GCAGGGAGCG GGGCCGATCC
    AAAGAGCGCA AGCACTTGCT GTCCCCTGAC ATCTCCCGCT GCAACTCAGA GGAGAGGAGT
    CCCCAGGCAC ACGATGAGTC CCCAGAGCGG CGGCGCGAGT CCCGGTCACC CAGCGAGGGC
    AGGTCGCAAA CGCCCAACCG GCAGGGAACG GGCTCCCTGA GCGAAAGCTC CATCCCCTCC
    ATCTCGGACA CCAGCACACC CCGGCGAGGC CGCCGCCAGC TCCCACCTGT GCCCCCAAAA
    CCGCGTCCCC TCCTCTCCTA CGCCTCCATG CTGCGGCACG CTGCCGGAGA CGCCTCACCA
    CCTCCCGATG AGACCGAAGG GGGGTCCCCG CTCCTCTCCA AGGAGCCCAA CGCCACCGGC
    CTGACCGAGT CTTCCAGCTC CCCGGCGGGG AAGCCGAGCC GGCCGTCCAC CCCGCAGCGT
    TACATCTCGG AGCCCTACCT GGCCCTGCAC GACGACTCGC ACGCTTCGGA CTGCGGCGAG
    GAGGAGACGC TCACCTTCGA GGCGGCCGTG GCCACCAGTC TGGGTCGTTC CAACACCATC
    GGCTCGGCCC CACCGCTGCG ACACAGCTGG CAGATGCCCA ACGGGCACTA CCGGAGACGG
    AGGCGAGGGG CCGGGCAGGG CGTGATGTGC GGGGCCAGCA TCGATGTGCT CAGCGACACC
    GAGGAAGATG ACAAGTGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145967.2
    CDS128..7348
    Translation

    Target ORF information:

    RefSeq Version XM_015290481.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290481.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAGC GGGAGCGGCG CCGGAGGCAC
    CACATGTCGG TGTGGGAGCA GCGCACCAGC CAGCTGCGCC GGCACATGCA GATGTCCAGC
    CAGGAGGGCA TCAATAAGGA TGAGCCACCC CTCATCAACC CACATGCCAG CATCTTCCGC
    CGCAAGAAGC CGGGGGACGG TGTTGCCTTG GAGAAGTGCG ATGAAGAGCA AGGCGGCAAG
    GGTGAGCGGC CGCCAGCTGA GGGTCCCGAG CAGCCGGCCC CAGGGACCAA CCCAGGCGGT
    GGGGAGGACA GGAAGAGCCC ATCGCCCCGT GCCAGGAGGG ACAAGGAGAT GTGGCAACAC
    AAAGCGTGCC ACGGGAACTG CGAGCCGGGT GAGGAAGGGG CAGGAGGTGG CATCGAGGAG
    AGGGCGCGGA TGAGACAGAG CCAGCGGCGC AGCCGGCATC GGAAAGCCAG GATGGAGGGG
    AAGGAGACGA CCGGAGCCCT GGAGAGCCGT GCGGGCAGCC AGGAGATGGG GCTGGAGGAG
    CCCTGCCCCG CAGAGGGGAT GCAGGATGGG GAGCGGCGTG GGGATTCTGC TGCCGCGCTG
    GACCTGATTC AGGGCGAGCT GGAGGCGAGC GGGGACCCCA CCAGGACTAA TGGGGTTCCC
    GCCGAGGAGG GAAGCCTCCC AGCATCAGCC CCTGAGCCTT CCCGGGGGAT GGAGGGCAGC
    CTGGGTGAGC AGGACTGCAG CAGCCCAGAC ACCAGTGAGC AGGCCCTGCT GGGGGGGGCG
    GCACTGGGGG CCAGCCGCAC CGTCAGCCAC AGCGAGCCTG ACCTGTCCTC CGTCACCGCC
    AACACTGAGA AGGCCACCGA GAGCACCACC ATCATGATTG ATGTCCAAGA CTCCACTGTG
    GTACAGATTA GCAACAAGAC TGATGGAGAA GCCAGCCCCT TGAAGGAGGC AGAGAGCAAA
    GAGGATGAGG AAGAGATGGA GAAGAAGAAG CGCAAGAAGG AGAAGAGCGA GACGGGCAAA
    GCGATGGTGC CACACAGCTC CATGTTCATC TTCAGCACCA CAAACCCGGT GCGCAGAGCC
    TGCCACTACA TTGTGAACCT GCGCTACTTT GAGATGTGCA TCCTCCTGGT CATCGCCGCC
    AGCAGCATCG CCCTCGCGGC CGAGGATCCC GTCCTCACCA ACTCCGACCG TAACAAAGTG
    CTGAGGTATT TTGATTACGT TTTCACCGGT GTCTTCACCT TCGAGATGGT TATCAAGATG
    ATCGACCAGG GCTTGATCCT GCAGGATGGC TCCTACTTCC GAGACCTCTG GAACATCCTG
    GATTTCATCG TGGTGGTGGG AGCTCTGGTG GCCTTTGCCT TGGCGAATGC TTTGGGGACC
    AACAAGGGTC GGGATATCAA GACTATCAAG TCTCTCCGTG TGCTGCGGGT CTTGAGGCCC
    CTGAAAACCA TCAAGCGGCT GCCGAAGCTC AAGGCTGTCT TTGACTGTGT TGTGACATCC
    CTCAAGAACG TCTTCAACAT CCTCATTGTC TACAAGCTCT TCATGTTCAT CTTTGCTGTC
    ATCGCCGTCC AGCTCTTCAA GGGGAAGTTT TTCTACTGCA CTGACAGCTC TAAGGACACA
    GAGAAGGACT GCATAGGGAA CTATGTGGAC CATGAGAAGA ACAAGATGGA GGTGAAGTGC
    CGGGAGTGGA AACGACATGA GTTTCACTAT GACAATATAA TCTGGGCGTT GCTCACCCTG
    TTCACAGTCT CCACCGGCGA GGGTTGGCCC CAGGTGCTGC AGCATTCGGT GGATGTGACG
    GAGGAGGACC GTGGGCCCAG CCGCAGTAAC CGCATGGAGA TGTCCATTTT TTACGTTGTC
    TATTTTGTGG TCTTCCCTTT CTTCTTCGTC AACATTTTTG TGGCTCTCAT CATCATCACA
    TTCCAAGAGC AAGGAGACAA AATGATGGAG GAGTGCAGTC TGGAGAAGAA CGAGAGGGCC
    TGTATTGACT TTGCCATCAG TGCCAAGCCC CTCACACGCT ACATGCCCCA GAACCGGCAC
    ACCTTCCAGT ATCGGGTCTG GCACTTTGTG GTCTCTCCAT CCTTTGAGTA TACCATCATG
    GCCATGATTG CTTTGAACAC TGTGGTGCTG ATGATGAAGT ACTACTCTGC TCCATACACC
    TATGAGCTGG CCCTGAAGTA CCTGAACATC GCTTTCACCA TGGTGTTCTC TTTGGAGTGC
    GTCCTTAAGA TCATTGCTTT TGGCTTCCTG AATTATTTCC GGGATACCTG GAATATCTTT
    GACTTCATCA CCGTCATCGG CAGCATCACA GAGATCATCC TGACAGACAC CAAGCTGGTG
    AACACCAGTA GCTTCAACAT GAGTTTCCTG AAACTCTTCC GGGCGGCTCG GCTCATCAAG
    CTGCTGCGCC AGGGTTACAC CATCCGCATC CTGCTGTGGA CGTTCGTGCA GTCCTTCAAG
    GCTCTCCCCT ATGTCTGCCT CCTAATCGCG ATGCTTTTCT TCATCTATGC CATCATTGGG
    ATGCAGGTTT TCGGCAACAT CAAACTGGAT GAGGAAAGCC ACATTAACCG GCACAACAAC
    TTCCGCAGCT TCCTGGGCTC CCTCATGCTC CTCTTCAGGA GTGCCACCGG TGAGGCATGG
    CAGGAGATCA TGCTCTCCTG CCTGGAGGGC AAGGGCTGCG AGCCGGACAC GACAGCGACG
    TCCGGGCAGA ATGAGAACGA GCGCTGCGGC ACCGACCTGG CCTACGTCTA CTTTGTCTCC
    TTCATCTTCT TCTGCTCATT CCTGATGCTC AACCTGTTCG TGGCGGTCAT CATGGACAAT
    TTTGAGTACC TGACTCGAGA TTCCTCCATC CTGGGACCTC ACCATCTAGA TGAATTTGTC
    CGGATCTGGG CAGAGTATGA CAGAGCAGCG TGTGGCCGAA TCCATTACAC TGAGATGTAT
    GAAATGCTGA CTCTTATGTC ACCACCGCTA GGCCTCGGCA AGAGATGTCC TTCCAAAGTG
    GCCTATAAGA GACTGGTGCT GATGAACATG CCCGTGGCCG AGGACATGAC GGTCCATTTC
    ACCTCCACCC TGATGGCGCT GATACGCACC GCCCTGGACA TCAAAATTGC CAAAGGTGGT
    GCAGATTGGC AGCAGTTAGA CTCTGAGCTT CAAAAGGAGA TCCTGACCAT TTGGCCTCAC
    CTGTCCCAGA AGATGCTTGA CCTGTTGGTA CCCATGCCAA AAACCTCTGA CCTGACTGTT
    GGCAAAATAT ATGCAGCCAT GATGATCATG GATTACTACA AGCAGAGCAA GGCGAAGAAG
    CAGAGGCAGC AGCTGGAGGA GCAGAAAAAT GCGCCCATGT TCCAGCGCAT GGAGCCATCC
    TCTTTGCCAC AGGAAATCAT CTCCAACGCA AAGGCTCTGC CTTACCTCCA GCAGGAGACC
    CTCACAGGGC TGAGCAGCCG GAGCGGGTTC CCCTCGCTGA GCCCGCTCTC ACCACAGGAG
    ATCTTCCAGC TGGCGTGCAT GGATCCGGCC CACGGGCAGT TCCAGGAGCA CCAGTCTCTG
    GAGCCAGAGG TTAGGGAGTT TCAAAGAGTG CAGCCCTCTA ACCGTGGCAG CTACCTTCCC
    GTGGACACTC AGGACCACGC TGTCTCTGGG AGGGCCTCCT CCATGCCTCG TCTCACTGTG
    GATCCCCAGG TGGTGACGGA CACCAGCTCG ATGCGGCGGT CATTTTCCAC CATCCGGGAC
    AAACGCACCA ACACCTCCTG GCTGGATGAG TTCTCCATGG AGCGCAGCAG CGACAACACC
    TACAAGTCCC GCCGGCGCAG CTACCACTCC TCTCTCCAGC TCTCTGCCCG GCGCCTCAAC
    GCTGACTCAG GCCACAGGTC TGAGGGGCAC CGCTCGGGCG GCAGGGAGCG GGGCCGATCC
    AAAGAGCGCA AGCACTTGCT GTCCCCTGAC ATCTCCCGCT GCAACTCAGA GGAGAGGAGT
    CCCCAGGCAC ACGATGAGTC CCCAGAGCGG CGGCGCGAGT CCCGGTCACC CAGCGAGGGC
    AGGTCGCAAA CGCCCAACCG GCAGGGAACG GGCTCCCTGA GCGAAAGCTC CATCCCCTCC
    ATCTCGGACA CCAGCACACC CCGGCGAGGC CGCCGCCAGC TCCCACCTGT GCCCCCAAAA
    CCGCGTCCCC TCCTCTCCTA CGCCTCCATG CTGCGGCACG CTGCCGGAGA CGCCTCACCA
    CCTCCCGATG AGACCGAAGG GGGGTCCCCG CTCCTCTCCA AGGAGCCCAA CGCCACCGGC
    CTGACCGAGT CTTCCAGCTC CCCGGCGGGG AAGCCGAGCC GGCCGTCCAC CCCGCAGCGT
    TACATCTCGG AGCCCTACCT GGCCCTGCAC GACGACTCGC ACGCTTCGGA CTGCGGCGAG
    GAGGAGACGC TCACCTTCGA GGCGGCCGTG GCCACCAGTC TGGGTCGTTC CAACACCATC
    GGCTCGGCCC CACCGCTGCG ACACAGCTGG CAGATGCCCA ACGGGCACTA CCGGAGACGG
    AGGCGAGGGG CCGGGCAGGG CGTGATGTGC GGGGCCAGCA TCGATGTGCT CAGCGACACC
    GAGGAAGATG ACAAGTGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152511
    Clone ID Related Accession (Same CDS sequence) XM_015290482.2
    Accession Version XM_015290482.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATTTTTGTGG CTCTCATCAT CATCACATTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CGGTCATCAT GGACAATTTT
    GAGTACCTGA CTCGAGATTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGT GGCCGAATCC ATTACACTGA GATGTATGAA
    ATGCTGACTC TTATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCTTC CAAAGTGGCC
    TATAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGTG
    GTGACGGACA CCAGCTCGAT GCGGCGGTCA TTTTCCACCA TCCGGGACAA ACGCACCAAC
    ACCTCCTGGC TGGATGAGTT CTCCATGGAG CGCAGCAGCG ACAACACCTA CAAGTCCCGC
    CGGCGCAGCT ACCACTCCTC TCTCCAGCTC TCTGCCCGGC GCCTCAACGC TGACTCAGGC
    CACAGGTCTG AGGGGCACCG CTCGGGCGGC AGGGAGCGGG GCCGATCCAA AGAGCGCAAG
    CACTTGCTGT CCCCTGACAT CTCCCGCTGC AACTCAGAGG AGAGGAGTCC CCAGGCACAC
    GATGAGTCCC CAGAGCGGCG GCGCGAGTCC CGGTCACCCA GCGAGGGCAG GTCGCAAACG
    CCCAACCGGC AGGGAACGGG CTCCCTGAGC GAAAGCTCCA TCCCCTCCAT CTCGGACACC
    AGCACACCCC GGCGAGGCCG CCGCCAGCTC CCACCTGTGC CCCCAAAACC GCGTCCCCTC
    CTCTCCTACG CCTCCATGCT GCGGCACGCT GCCGGAGACG CCTCACCACC TCCCGATGAG
    ACCGAAGGGG GGTCCCCGCT CCTCTCCAAG GAGCCCAACG CCACCGGCCT GACCGAGTCT
    TCCAGCTCCC CGGCGGGGAA GCCGAGCCGG CCGTCCACCC CGCAGCGTTA CATCTCGGAG
    CCCTACCTGG CCCTGCACGA CGACTCGCAC GCTTCGGACT GCGGCGAGGA GGAGACGCTC
    ACCTTCGAGG CGGCCGTGGC CACCAGTCTG GGTCGTTCCA ACACCATCGG CTCGGCCCCA
    CCGCTGCGAC ACAGCTGGCA GATGCCCAAC GGGCACTACC GGAGACGGAG GCGAGGGGCC
    GGGCAGGGCG TGATGTGCGG GGCCAGCATC GATGTGCTCA GCGACACCGA GGAAGATGAC
    AAGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145968.2
    CDS127..7275
    Translation

    Target ORF information:

    RefSeq Version XM_015290482.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290482.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATTTTTGTGG CTCTCATCAT CATCACATTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CGGTCATCAT GGACAATTTT
    GAGTACCTGA CTCGAGATTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGT GGCCGAATCC ATTACACTGA GATGTATGAA
    ATGCTGACTC TTATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCTTC CAAAGTGGCC
    TATAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGTG
    GTGACGGACA CCAGCTCGAT GCGGCGGTCA TTTTCCACCA TCCGGGACAA ACGCACCAAC
    ACCTCCTGGC TGGATGAGTT CTCCATGGAG CGCAGCAGCG ACAACACCTA CAAGTCCCGC
    CGGCGCAGCT ACCACTCCTC TCTCCAGCTC TCTGCCCGGC GCCTCAACGC TGACTCAGGC
    CACAGGTCTG AGGGGCACCG CTCGGGCGGC AGGGAGCGGG GCCGATCCAA AGAGCGCAAG
    CACTTGCTGT CCCCTGACAT CTCCCGCTGC AACTCAGAGG AGAGGAGTCC CCAGGCACAC
    GATGAGTCCC CAGAGCGGCG GCGCGAGTCC CGGTCACCCA GCGAGGGCAG GTCGCAAACG
    CCCAACCGGC AGGGAACGGG CTCCCTGAGC GAAAGCTCCA TCCCCTCCAT CTCGGACACC
    AGCACACCCC GGCGAGGCCG CCGCCAGCTC CCACCTGTGC CCCCAAAACC GCGTCCCCTC
    CTCTCCTACG CCTCCATGCT GCGGCACGCT GCCGGAGACG CCTCACCACC TCCCGATGAG
    ACCGAAGGGG GGTCCCCGCT CCTCTCCAAG GAGCCCAACG CCACCGGCCT GACCGAGTCT
    TCCAGCTCCC CGGCGGGGAA GCCGAGCCGG CCGTCCACCC CGCAGCGTTA CATCTCGGAG
    CCCTACCTGG CCCTGCACGA CGACTCGCAC GCTTCGGACT GCGGCGAGGA GGAGACGCTC
    ACCTTCGAGG CGGCCGTGGC CACCAGTCTG GGTCGTTCCA ACACCATCGG CTCGGCCCCA
    CCGCTGCGAC ACAGCTGGCA GATGCCCAAC GGGCACTACC GGAGACGGAG GCGAGGGGCC
    GGGCAGGGCG TGATGTGCGG GGCCAGCATC GATGTGCTCA GCGACACCGA GGAAGATGAC
    AAGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152512
    Clone ID Related Accession (Same CDS sequence) XM_015290483.2
    Accession Version XM_015290483.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290483.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATTTTTGTGG CTCTCATCAT CATCACATTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CGGTCATCAT GGACAATTTT
    GAGTACCTGA CTCGAGATTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGC GGCAGGATCT CGTATAAGGA TATGTACAAA
    TTAGTACGGG TGATCTCGCC TCCTCTGGGC CTAGGAGAGA ACTGCCCCTA CAGGGTGGCG
    TGCAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGTG
    GTGACGGACA CCAGCTCGAT GCGGCGGTCA TTTTCCACCA TCCGGGACAA ACGCACCAAC
    ACCTCCTGGC TGGATGAGTT CTCCATGGAG CGCAGCAGCG ACAACACCTA CAAGTCCCGC
    CGGCGCAGCT ACCACTCCTC TCTCCAGCTC TCTGCCCGGC GCCTCAACGC TGACTCAGGC
    CACAGGTCTG AGGGGCACCG CTCGGGCGGC AGGGAGCGGG GCCGATCCAA AGAGCGCAAG
    CACTTGCTGT CCCCTGACAT CTCCCGCTGC AACTCAGAGG AGAGGAGTCC CCAGGCACAC
    GATGAGTCCC CAGAGCGGCG GCGCGAGTCC CGGTCACCCA GCGAGGGCAG GTCGCAAACG
    CCCAACCGGC AGGGAACGGG CTCCCTGAGC GAAAGCTCCA TCCCCTCCAT CTCGGACACC
    AGCACACCCC GGCGAGGCCG CCGCCAGCTC CCACCTGTGC CCCCAAAACC GCGTCCCCTC
    CTCTCCTACG CCTCCATGCT GCGGCACGCT GCCGGAGACG CCTCACCACC TCCCGATGAG
    ACCGAAGGGG GGTCCCCGCT CCTCTCCAAG GAGCCCAACG CCACCGGCCT GACCGAGTCT
    TCCAGCTCCC CGGCGGGGAA GCCGAGCCGG CCGTCCACCC CGCAGCGTTA CATCTCGGAG
    CCCTACCTGG CCCTGCACGA CGACTCGCAC GCTTCGGACT GCGGCGAGGA GGAGACGCTC
    ACCTTCGAGG CGGCCGTGGC CACCAGTCTG GGTCGTTCCA ACACCATCGG CTCGGCCCCA
    CCGCTGCGAC ACAGCTGGCA GATGCCCAAC GGGCACTACC GGAGACGGAG GCGAGGGGCC
    GGGCAGGGCG TGATGTGCGG GGCCAGCATC GATGTGCTCA GCGACACCGA GGAAGATGAC
    AAGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145969.2
    CDS128..7276
    Translation

    Target ORF information:

    RefSeq Version XM_015290483.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290483.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATTTTTGTGG CTCTCATCAT CATCACATTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CGGTCATCAT GGACAATTTT
    GAGTACCTGA CTCGAGATTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGC GGCAGGATCT CGTATAAGGA TATGTACAAA
    TTAGTACGGG TGATCTCGCC TCCTCTGGGC CTAGGAGAGA ACTGCCCCTA CAGGGTGGCG
    TGCAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGTG
    GTGACGGACA CCAGCTCGAT GCGGCGGTCA TTTTCCACCA TCCGGGACAA ACGCACCAAC
    ACCTCCTGGC TGGATGAGTT CTCCATGGAG CGCAGCAGCG ACAACACCTA CAAGTCCCGC
    CGGCGCAGCT ACCACTCCTC TCTCCAGCTC TCTGCCCGGC GCCTCAACGC TGACTCAGGC
    CACAGGTCTG AGGGGCACCG CTCGGGCGGC AGGGAGCGGG GCCGATCCAA AGAGCGCAAG
    CACTTGCTGT CCCCTGACAT CTCCCGCTGC AACTCAGAGG AGAGGAGTCC CCAGGCACAC
    GATGAGTCCC CAGAGCGGCG GCGCGAGTCC CGGTCACCCA GCGAGGGCAG GTCGCAAACG
    CCCAACCGGC AGGGAACGGG CTCCCTGAGC GAAAGCTCCA TCCCCTCCAT CTCGGACACC
    AGCACACCCC GGCGAGGCCG CCGCCAGCTC CCACCTGTGC CCCCAAAACC GCGTCCCCTC
    CTCTCCTACG CCTCCATGCT GCGGCACGCT GCCGGAGACG CCTCACCACC TCCCGATGAG
    ACCGAAGGGG GGTCCCCGCT CCTCTCCAAG GAGCCCAACG CCACCGGCCT GACCGAGTCT
    TCCAGCTCCC CGGCGGGGAA GCCGAGCCGG CCGTCCACCC CGCAGCGTTA CATCTCGGAG
    CCCTACCTGG CCCTGCACGA CGACTCGCAC GCTTCGGACT GCGGCGAGGA GGAGACGCTC
    ACCTTCGAGG CGGCCGTGGC CACCAGTCTG GGTCGTTCCA ACACCATCGG CTCGGCCCCA
    CCGCTGCGAC ACAGCTGGCA GATGCCCAAC GGGCACTACC GGAGACGGAG GCGAGGGGCC
    GGGCAGGGCG TGATGTGCGG GGCCAGCATC GATGTGCTCA GCGACACCGA GGAAGATGAC
    AAGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152513
    Clone ID Related Accession (Same CDS sequence) XM_015290484.2
    Accession Version XM_015290484.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6879bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290484.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCTCGCT TCGGGGAGGC GGTGGCTGGC AGGCTGGGCT CCGGGGATGG GGGCTCCGAG 
    CAGAACCGCA GCCGGCAAGG AGCCGCTGCT CCGGCGGGAG GGTCCCCGGG AGCCTTCAAG
    CAAACCAAAG CGCAGCGAGC CCGGACTATG GCTCTGTACA ACCCCATCCC CGTCCGGCAG
    AACTGCTTCA CCGTCAACAG ATCGTTGTTC CTCTTTGGGG AAGATAACAT AGTCAGGAAA
    TACGCCAAGA AGCTCATCGA CTGGCCTCCC TTCGAGTATA TGATCCTGGC AACCATAATT
    GCCAACTGCA TCGTGCTGGC CCTGGAGCAG CACCTCCCAG AGGACGACAA GACACCCATG
    TCACGGAGAC TGGAGAAGAC GGAGCCGTAC TTTATTGGGA TTTTCTGCTT TGAGGCTGGG
    ATTAAGATCG TGGCTCTGGG CTTCGTTTTC CACAAGGGCT CATACCTACG CAATGGCTGG
    AACGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTTG CCACCGCTGG AACACACTTC
    AACACGCACG TAGACCTGCG CACCCTGCGG GCCGTGCGTG TGCTCAGGCC TCTGAAGCTC
    GTCTCGGGCA TTCCCAGCCT TCAGATTGTG CTGAAATCCA TCATGAAGGC CATGGTACCT
    CTCCTCCAGA TTGGCTTGCT GCTCTTTTTC GCTATTCTCA TGTTTGCCAT CATCGGCCTG
    GAGTTTTACA GTGGGAAACT GCACCGAGCC TGCTACACAA ACAATTCAGG TGAGCTGGAG
    GAGCTGGACC CTCCCCACCC CTGCGGGGTG CAGGGCTGTC CGCCGGGCTA TGAGTGCCGG
    GAGTGGATTG GTCCCAATGA TGGGATCACG CAGTTCGACA ACATCCTCTT TGCTGTGCTG
    ACCGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACAG TTCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA CTGGCTATAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTCGTCCTCA ACCTTGTCCT GGGAGTGCTG TCAGGGGAGT TTGCCAAAGA ACGTGAGCGC
    GTGGAGAACC GCCGGGCCTT CATGAAGCTG CGGCGACAGC AGCAGATCGA GAGGGAGCTC
    AACGGCTACC GAGCCTGGAT AGACAAAGCG GAGGAAGTCA TGCTGGCTGA AGAGAACAAA
    AACGCTGGGA CCTCAGCCTT AGAAGTGCTC AGGAGAGCCA CCATCAAGAG GAACCGGACG
    GATGCCATGA ACCGTGACTC CAGCGATGAG CACTGCGTCG ATATCTCCTC CGTGGGCGAG
    CGGAGGCTGG CACAGAGGAG GCCTCTTCAT CCCACCTTGG GTAACCCCTT GAGTCGCTCC
    GGTCTCAAAG GAGTAGATGG AGCCTCCTAT TTACGGCACA AGGAGCGGCT CCTGCGCATT
    TCTGTTCGTC ACATGGTGAA ATCCCAGGTC TTCTACTGGA TTGTCTTGAG CCTGGTGGCT
    CTTAACACTG CCTGTGTGGC CATCGTCCAT CACAACCAGC CAGCCTGGCT CACCCACTTC
    CTCTACTATG CAGAGTTCCT GTTTCTTGGG CTCTTCCTCC TGGAGATGTC CTTGAAGATG
    TATGGGATGG GGCCACGCCT GTACTTCCAT TCTTCATTCA ACTGTTTCGA CTGTGGGGTC
    ACAGTGGGAA GCATCTTTGA AGTGGTCTGG GCCATCTTCC GACCAGGGAC CTCTTTTGGG
    ATCAGTGTCC TACGAGCCCT TCGTCTCCTG CGAATATTTA AAATAACCAA GTACTGGGCA
    TCCTTGAGGA ATTTGGTGGT CTCGCTGATG AGCTCTATGA AATCTATTAT CAGCCTGCTC
    TTCTTGCTCT TTCTCTTCAT TGTAGTCTTT GCCCTGCTGG GAATGCAGCT GTTTGGAGGC
    GGGTTTAATT TCATTGATGG GACACCATCG GCCAACTTTG ACACTTTCCC TGCAGCCATC
    ATGACGGTGT TCCAGATCTT GACGGGTGAG GACTGGAATG AGGTGATGTA CAATGGCATC
    CGCTCCCAGG GAGGTGTTCG CTCGGGCATG TGGTCCTCCA TCTATTTCAT CGTCCTCACC
    TTGTTTGGGA ACTATACTCT GCTGAATGTC TTCTTGGCCA TTGCAGTGGA CAACCTGGCT
    AATGCTCAGG AGCTCACCAA GGACGAGCAG GAGGAAGAGG AGGCCTTTAA CCAGAAGCAT
    GCCCTTCAGA AAGCCAAGGA AGTCAGCCCC ATGTCTGCCC CCAACATGCC TGCTATTGAA
    AGAGACAGGC GCAGGAGACA CCACATGTCG ATGTGGGAGC CGCGCAGCAG CCACCTGCGG
    GAGCGGCGCC GGAGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGCGCCGG
    CACATGCAGA TGTCCAGCCA GGAGGGCATC AATAAGGATG AGCCACCCCT CATCAACCCA
    CATGCCAGCA TCTTCCGCCG CAAGAAGCCG GGGGACGGTG TTGCCTTGGA GAAGTGCGAT
    GAAGAGCAAG GCGGCAAGGG TGAGCGGCCG CCAGCTGAGG GTCCCGAGCA GCCGGCCCCA
    GGGACCAACC CAGGCGGTGG GGAGGACAGG AAGAGCCCAT CGCCCCGTGC CAGGAGGGAC
    AAGGAGATGT GGCAACACAA AGCGTGCCAC GGGAACTGCG AGCCGGGTGA GGAAGGGGCA
    GGAGGTGGCA TCGAGGAGAG GGCGCGGATG AGACAGAGCC AGCGGCGCAG CCGGCATCGG
    AAAGCCAGGA TGGAGGGGAA GGAGACGACC GGAGCCCTGG AGAGCCGTGC GGGCAGCCAG
    GAGATGGGGC TGGAGGAGCC CTGCCCCGCA GAGGGGATGC AGGATGGGGA GCGGCGTGGG
    GATTCTGCTG CCGCGCTGGA CCTGATTCAG GGCGAGCTGG AGGCGAGCGG GGACCCCACC
    AGGACTAATG GGGTTCCCGC CGAGGAGGGA AGCCTCCCAG CATCAGCCCC TGAGCCTTCC
    CGGGGGATGG AGGGCAGCCT GGGTGAGCAG GACTGCAGCA GCCCAGACAC CAGTGAGCAG
    GCCCTGCTGG GGGGGGCGGC ACTGGGGGCC AGCCGCACCG TCAGCCACAG CGAGCCTGAC
    CTGTCCTCCG TCACCGCCAA CACTGAGAAG GCCACCGAGA GCACCACCAT CATGATTGAT
    GTCCAAGACT CCACTGTGGT ACAGATTAGC AACAAGACTG ATGGAGAAGC CAGCCCCTTG
    AAGGAGGCAG AGAGCAAAGA GGATGAGGAA GAGATGGAGA AGAAGAAGCG CAAGAAGGAG
    AAGAGCGAGA CGGGCAAAGC GATGGTGCCA CACAGCTCCA TGTTCATCTT CAGCACCACA
    AACCCGGTGC GCAGAGCCTG CCACTACATT GTGAACCTGC GCTACTTTGA GATGTGCATC
    CTCCTGGTCA TCGCCGCCAG CAGCATCGCC CTCGCGGCCG AGGATCCCGT CCTCACCAAC
    TCCGACCGTA ACAAAGTGCT GAGGTATTTT GATTACGTTT TCACCGGTGT CTTCACCTTC
    GAGATGGTTA TCAAGATGAT CGACCAGGGC TTGATCCTGC AGGATGGCTC CTACTTCCGA
    GACCTCTGGA ACATCCTGGA TTTCATCGTG GTGGTGGGAG CTCTGGTGGC CTTTGCCTTG
    GCGAATGCTT TGGGGACCAA CAAGGGTCGG GATATCAAGA CTATCAAGTC TCTCCGTGTG
    CTGCGGGTCT TGAGGCCCCT GAAAACCATC AAGCGGCTGC CGAAGCTCAA GGCTGTCTTT
    GACTGTGTTG TGACATCCCT CAAGAACGTC TTCAACATCC TCATTGTCTA CAAGCTCTTC
    ATGTTCATCT TTGCTGTCAT CGCCGTCCAG CTCTTCAAGG GGAAGTTTTT CTACTGCACT
    GACAGCTCTA AGGACACAGA GAAGGACTGC ATAGGGAACT ATGTGGACCA TGAGAAGAAC
    AAGATGGAGG TGAAGTGCCG GGAGTGGAAA CGACATGAGT TTCACTATGA CAATATAATC
    TGGGCGTTGC TCACCCTGTT CACAGTCTCC ACCGGCGAGG GTTGGCCCCA GGTGCTGCAG
    CATTCGGTGG ATGTGACGGA GGAGGACCGT GGGCCCAGCC GCAGTAACCG CATGGAGATG
    TCCATTTTTT ACGTTGTCTA TTTTGTGGTC TTCCCTTTCT TCTTCGTCAA CATTTTTGTG
    GCTCTCATCA TCATCACATT CCAAGAGCAA GGAGACAAAA TGATGGAGGA GTGCAGTCTG
    GAGAAGAACG AGAGGGCCTG TATTGACTTT GCCATCAGTG CCAAGCCCCT CACACGCTAC
    ATGCCCCAGA ACCGGCACAC CTTCCAGTAT CGGGTCTGGC ACTTTGTGGT CTCTCCATCC
    TTTGAGTATA CCATCATGGC CATGATTGCT TTGAACACTG TGGTGCTGAT GATGAAGTAC
    TACTCTGCTC CATACACCTA TGAGCTGGCC CTGAAGTACC TGAACATCGC TTTCACCATG
    GTGTTCTCTT TGGAGTGCGT CCTTAAGATC ATTGCTTTTG GCTTCCTGAA TTATTTCCGG
    GATACCTGGA ATATCTTTGA CTTCATCACC GTCATCGGCA GCATCACAGA GATCATCCTG
    ACAGACACCA AGCTGGTGAA CACCAGTAGC TTCAACATGA GTTTCCTGAA ACTCTTCCGG
    GCGGCTCGGC TCATCAAGCT GCTGCGCCAG GGTTACACCA TCCGCATCCT GCTGTGGACG
    TTCGTGCAGT CCTTCAAGGC TCTCCCCTAT GTCTGCCTCC TAATCGCGAT GCTTTTCTTC
    ATCTATGCCA TCATTGGGAT GCAGGTTTTC GGCAACATCA AACTGGATGA GGAAAGCCAC
    ATTAACCGGC ACAACAACTT CCGCAGCTTC CTGGGCTCCC TCATGCTCCT CTTCAGGAGT
    GCCACCGGTG AGGCATGGCA GGAGATCATG CTCTCCTGCC TGGAGGGCAA GGGCTGCGAG
    CCGGACACGA CAGCGACGTC CGGGCAGAAT GAGAACGAGC GCTGCGGCAC CGACCTGGCC
    TACGTCTACT TTGTCTCCTT CATCTTCTTC TGCTCATTCC TGATGCTCAA CCTGTTCGTG
    GCGGTCATCA TGGACAATTT TGAGTACCTG ACTCGAGATT CCTCCATCCT GGGACCTCAC
    CATCTAGATG AATTTGTCCG GATCTGGGCA GAGTATGACA GAGCAGCGTG TGGCCGAATC
    CATTACACTG AGATGTATGA AATGCTGACT CTTATGTCAC CACCGCTAGG CCTCGGCAAG
    AGATGTCCTT CCAAAGTGGC CTATAAGAGA CTGGTGCTGA TGAACATGCC CGTGGCCGAG
    GACATGACGG TCCATTTCAC CTCCACCCTG ATGGCGCTGA TACGCACCGC CCTGGACATC
    AAAATTGCCA AAGGTGGTGC AGATTGGCAG CAGTTAGACT CTGAGCTTCA AAAGGAGATC
    CTGACCATTT GGCCTCACCT GTCCCAGAAG ATGCTTGACC TGTTGGTACC CATGCCAAAA
    ACCTCTGACC TGACTGTTGG CAAAATATAT GCAGCCATGA TGATCATGGA TTACTACAAG
    CAGAGCAAGG CGAAGAAGCA GAGGCAGCAG CTGGAGGAGC AGAAAAATGC GCCCATGTTC
    CAGCGCATGG AGCCATCCTC TTTGCCACAG GAAATCATCT CCAACGCAAA GGCTCTGCCT
    TACCTCCAGC AGGAGACCCT CACAGGGCTG AGCAGCCGGA GCGGGTTCCC CTCGCTGAGC
    CCGCTCTCAC CACAGGAGAT CTTCCAGCTG GCGTGCATGG ATCCGGCCCA CGGGCAGTTC
    CAGGAGCACC AGTCTCTGGA GCCAGAGGTT AGGGAGTTTC AAAGAGTGCA GCCCTCTAAC
    CGTGGCAGCT ACCTTCCCGT GGACACTCAG GACCACGCTG TCTCTGGGAG GGCCTCCTCC
    ATGCCTCGTC TCACTGTGGA TCCCCAGGTG GTGACGGACA CCAGCTCGAT GCGGCGGTCA
    TTTTCCACCA TCCGGGACAA ACGCACCAAC ACCTCCTGGC TGGATGAGTT CTCCATGGAG
    CGCAGCAGCG ACAACACCTA CAAGTCCCGC CGGCGCAGCT ACCACTCCTC TCTCCAGCTC
    TCTGCCCGGC GCCTCAACGC TGACTCAGGC CACAGGTCTG AGGGGCACCG CTCGGGCGGC
    AGGGAGCGGG GCCGATCCAA AGAGCGCAAG CACTTGCTGT CCCCTGACAT CTCCCGCTGC
    AACTCAGAGG AGAGGAGTCC CCAGGCACAC GATGAGTCCC CAGAGCGGCG GCGCGAGTCC
    CGGTCACCCA GCGAGGGCAG GTCGCAAACG CCCAACCGGC AGGGAACGGG CTCCCTGAGC
    GAAAGCTCCA TCCCCTCCAT CTCGGACACC AGCACACCCC GGCGAGGCCG CCGCCAGCTC
    CCACCTGTGC CCCCAAAACC GCGTCCCCTC CTCTCCTACG CCTCCATGCT GCGGCACGCT
    GCCGGAGACG CCTCACCACC TCCCGATGAG ACCGAAGGGG GGTCCCCGCT CCTCTCCAAG
    GAGCCCAACG CCACCGGCCT GACCGAGTCT TCCAGCTCCC CGGCGGGGAA GCCGAGCCGG
    CCGTCCACCC CGCAGCGTTA CATCTCGGAG CCCTACCTGG CCCTGCACGA CGACTCGCAC
    GCTTCGGACT GCGGCGAGGA GGAGACGCTC ACCTTCGAGG CGGCCGTGGC CACCAGTCTG
    GGTCGTTCCA ACACCATCGG CTCGGCCCCA CCGCTGCGAC ACAGCTGGCA GATGCCCAAC
    GGGCACTACC GGAGACGGAG GCGAGGGGCC GGGCAGGGCG TGATGTGCGG GGCCAGCATC
    GATGTGCTCA GCGACACCGA GGAAGATGAC AAGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145970.2
    CDS429..7307
    Translation

    Target ORF information:

    RefSeq Version XM_015290484.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290484.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCTCGCT TCGGGGAGGC GGTGGCTGGC AGGCTGGGCT CCGGGGATGG GGGCTCCGAG 
    CAGAACCGCA GCCGGCAAGG AGCCGCTGCT CCGGCGGGAG GGTCCCCGGG AGCCTTCAAG
    CAAACCAAAG CGCAGCGAGC CCGGACTATG GCTCTGTACA ACCCCATCCC CGTCCGGCAG
    AACTGCTTCA CCGTCAACAG ATCGTTGTTC CTCTTTGGGG AAGATAACAT AGTCAGGAAA
    TACGCCAAGA AGCTCATCGA CTGGCCTCCC TTCGAGTATA TGATCCTGGC AACCATAATT
    GCCAACTGCA TCGTGCTGGC CCTGGAGCAG CACCTCCCAG AGGACGACAA GACACCCATG
    TCACGGAGAC TGGAGAAGAC GGAGCCGTAC TTTATTGGGA TTTTCTGCTT TGAGGCTGGG
    ATTAAGATCG TGGCTCTGGG CTTCGTTTTC CACAAGGGCT CATACCTACG CAATGGCTGG
    AACGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTTG CCACCGCTGG AACACACTTC
    AACACGCACG TAGACCTGCG CACCCTGCGG GCCGTGCGTG TGCTCAGGCC TCTGAAGCTC
    GTCTCGGGCA TTCCCAGCCT TCAGATTGTG CTGAAATCCA TCATGAAGGC CATGGTACCT
    CTCCTCCAGA TTGGCTTGCT GCTCTTTTTC GCTATTCTCA TGTTTGCCAT CATCGGCCTG
    GAGTTTTACA GTGGGAAACT GCACCGAGCC TGCTACACAA ACAATTCAGG TGAGCTGGAG
    GAGCTGGACC CTCCCCACCC CTGCGGGGTG CAGGGCTGTC CGCCGGGCTA TGAGTGCCGG
    GAGTGGATTG GTCCCAATGA TGGGATCACG CAGTTCGACA ACATCCTCTT TGCTGTGCTG
    ACCGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACAG TTCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA CTGGCTATAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTCGTCCTCA ACCTTGTCCT GGGAGTGCTG TCAGGGGAGT TTGCCAAAGA ACGTGAGCGC
    GTGGAGAACC GCCGGGCCTT CATGAAGCTG CGGCGACAGC AGCAGATCGA GAGGGAGCTC
    AACGGCTACC GAGCCTGGAT AGACAAAGCG GAGGAAGTCA TGCTGGCTGA AGAGAACAAA
    AACGCTGGGA CCTCAGCCTT AGAAGTGCTC AGGAGAGCCA CCATCAAGAG GAACCGGACG
    GATGCCATGA ACCGTGACTC CAGCGATGAG CACTGCGTCG ATATCTCCTC CGTGGGCGAG
    CGGAGGCTGG CACAGAGGAG GCCTCTTCAT CCCACCTTGG GTAACCCCTT GAGTCGCTCC
    GGTCTCAAAG GAGTAGATGG AGCCTCCTAT TTACGGCACA AGGAGCGGCT CCTGCGCATT
    TCTGTTCGTC ACATGGTGAA ATCCCAGGTC TTCTACTGGA TTGTCTTGAG CCTGGTGGCT
    CTTAACACTG CCTGTGTGGC CATCGTCCAT CACAACCAGC CAGCCTGGCT CACCCACTTC
    CTCTACTATG CAGAGTTCCT GTTTCTTGGG CTCTTCCTCC TGGAGATGTC CTTGAAGATG
    TATGGGATGG GGCCACGCCT GTACTTCCAT TCTTCATTCA ACTGTTTCGA CTGTGGGGTC
    ACAGTGGGAA GCATCTTTGA AGTGGTCTGG GCCATCTTCC GACCAGGGAC CTCTTTTGGG
    ATCAGTGTCC TACGAGCCCT TCGTCTCCTG CGAATATTTA AAATAACCAA GTACTGGGCA
    TCCTTGAGGA ATTTGGTGGT CTCGCTGATG AGCTCTATGA AATCTATTAT CAGCCTGCTC
    TTCTTGCTCT TTCTCTTCAT TGTAGTCTTT GCCCTGCTGG GAATGCAGCT GTTTGGAGGC
    GGGTTTAATT TCATTGATGG GACACCATCG GCCAACTTTG ACACTTTCCC TGCAGCCATC
    ATGACGGTGT TCCAGATCTT GACGGGTGAG GACTGGAATG AGGTGATGTA CAATGGCATC
    CGCTCCCAGG GAGGTGTTCG CTCGGGCATG TGGTCCTCCA TCTATTTCAT CGTCCTCACC
    TTGTTTGGGA ACTATACTCT GCTGAATGTC TTCTTGGCCA TTGCAGTGGA CAACCTGGCT
    AATGCTCAGG AGCTCACCAA GGACGAGCAG GAGGAAGAGG AGGCCTTTAA CCAGAAGCAT
    GCCCTTCAGA AAGCCAAGGA AGTCAGCCCC ATGTCTGCCC CCAACATGCC TGCTATTGAA
    AGAGACAGGC GCAGGAGACA CCACATGTCG ATGTGGGAGC CGCGCAGCAG CCACCTGCGG
    GAGCGGCGCC GGAGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGCGCCGG
    CACATGCAGA TGTCCAGCCA GGAGGGCATC AATAAGGATG AGCCACCCCT CATCAACCCA
    CATGCCAGCA TCTTCCGCCG CAAGAAGCCG GGGGACGGTG TTGCCTTGGA GAAGTGCGAT
    GAAGAGCAAG GCGGCAAGGG TGAGCGGCCG CCAGCTGAGG GTCCCGAGCA GCCGGCCCCA
    GGGACCAACC CAGGCGGTGG GGAGGACAGG AAGAGCCCAT CGCCCCGTGC CAGGAGGGAC
    AAGGAGATGT GGCAACACAA AGCGTGCCAC GGGAACTGCG AGCCGGGTGA GGAAGGGGCA
    GGAGGTGGCA TCGAGGAGAG GGCGCGGATG AGACAGAGCC AGCGGCGCAG CCGGCATCGG
    AAAGCCAGGA TGGAGGGGAA GGAGACGACC GGAGCCCTGG AGAGCCGTGC GGGCAGCCAG
    GAGATGGGGC TGGAGGAGCC CTGCCCCGCA GAGGGGATGC AGGATGGGGA GCGGCGTGGG
    GATTCTGCTG CCGCGCTGGA CCTGATTCAG GGCGAGCTGG AGGCGAGCGG GGACCCCACC
    AGGACTAATG GGGTTCCCGC CGAGGAGGGA AGCCTCCCAG CATCAGCCCC TGAGCCTTCC
    CGGGGGATGG AGGGCAGCCT GGGTGAGCAG GACTGCAGCA GCCCAGACAC CAGTGAGCAG
    GCCCTGCTGG GGGGGGCGGC ACTGGGGGCC AGCCGCACCG TCAGCCACAG CGAGCCTGAC
    CTGTCCTCCG TCACCGCCAA CACTGAGAAG GCCACCGAGA GCACCACCAT CATGATTGAT
    GTCCAAGACT CCACTGTGGT ACAGATTAGC AACAAGACTG ATGGAGAAGC CAGCCCCTTG
    AAGGAGGCAG AGAGCAAAGA GGATGAGGAA GAGATGGAGA AGAAGAAGCG CAAGAAGGAG
    AAGAGCGAGA CGGGCAAAGC GATGGTGCCA CACAGCTCCA TGTTCATCTT CAGCACCACA
    AACCCGGTGC GCAGAGCCTG CCACTACATT GTGAACCTGC GCTACTTTGA GATGTGCATC
    CTCCTGGTCA TCGCCGCCAG CAGCATCGCC CTCGCGGCCG AGGATCCCGT CCTCACCAAC
    TCCGACCGTA ACAAAGTGCT GAGGTATTTT GATTACGTTT TCACCGGTGT CTTCACCTTC
    GAGATGGTTA TCAAGATGAT CGACCAGGGC TTGATCCTGC AGGATGGCTC CTACTTCCGA
    GACCTCTGGA ACATCCTGGA TTTCATCGTG GTGGTGGGAG CTCTGGTGGC CTTTGCCTTG
    GCGAATGCTT TGGGGACCAA CAAGGGTCGG GATATCAAGA CTATCAAGTC TCTCCGTGTG
    CTGCGGGTCT TGAGGCCCCT GAAAACCATC AAGCGGCTGC CGAAGCTCAA GGCTGTCTTT
    GACTGTGTTG TGACATCCCT CAAGAACGTC TTCAACATCC TCATTGTCTA CAAGCTCTTC
    ATGTTCATCT TTGCTGTCAT CGCCGTCCAG CTCTTCAAGG GGAAGTTTTT CTACTGCACT
    GACAGCTCTA AGGACACAGA GAAGGACTGC ATAGGGAACT ATGTGGACCA TGAGAAGAAC
    AAGATGGAGG TGAAGTGCCG GGAGTGGAAA CGACATGAGT TTCACTATGA CAATATAATC
    TGGGCGTTGC TCACCCTGTT CACAGTCTCC ACCGGCGAGG GTTGGCCCCA GGTGCTGCAG
    CATTCGGTGG ATGTGACGGA GGAGGACCGT GGGCCCAGCC GCAGTAACCG CATGGAGATG
    TCCATTTTTT ACGTTGTCTA TTTTGTGGTC TTCCCTTTCT TCTTCGTCAA CATTTTTGTG
    GCTCTCATCA TCATCACATT CCAAGAGCAA GGAGACAAAA TGATGGAGGA GTGCAGTCTG
    GAGAAGAACG AGAGGGCCTG TATTGACTTT GCCATCAGTG CCAAGCCCCT CACACGCTAC
    ATGCCCCAGA ACCGGCACAC CTTCCAGTAT CGGGTCTGGC ACTTTGTGGT CTCTCCATCC
    TTTGAGTATA CCATCATGGC CATGATTGCT TTGAACACTG TGGTGCTGAT GATGAAGTAC
    TACTCTGCTC CATACACCTA TGAGCTGGCC CTGAAGTACC TGAACATCGC TTTCACCATG
    GTGTTCTCTT TGGAGTGCGT CCTTAAGATC ATTGCTTTTG GCTTCCTGAA TTATTTCCGG
    GATACCTGGA ATATCTTTGA CTTCATCACC GTCATCGGCA GCATCACAGA GATCATCCTG
    ACAGACACCA AGCTGGTGAA CACCAGTAGC TTCAACATGA GTTTCCTGAA ACTCTTCCGG
    GCGGCTCGGC TCATCAAGCT GCTGCGCCAG GGTTACACCA TCCGCATCCT GCTGTGGACG
    TTCGTGCAGT CCTTCAAGGC TCTCCCCTAT GTCTGCCTCC TAATCGCGAT GCTTTTCTTC
    ATCTATGCCA TCATTGGGAT GCAGGTTTTC GGCAACATCA AACTGGATGA GGAAAGCCAC
    ATTAACCGGC ACAACAACTT CCGCAGCTTC CTGGGCTCCC TCATGCTCCT CTTCAGGAGT
    GCCACCGGTG AGGCATGGCA GGAGATCATG CTCTCCTGCC TGGAGGGCAA GGGCTGCGAG
    CCGGACACGA CAGCGACGTC CGGGCAGAAT GAGAACGAGC GCTGCGGCAC CGACCTGGCC
    TACGTCTACT TTGTCTCCTT CATCTTCTTC TGCTCATTCC TGATGCTCAA CCTGTTCGTG
    GCGGTCATCA TGGACAATTT TGAGTACCTG ACTCGAGATT CCTCCATCCT GGGACCTCAC
    CATCTAGATG AATTTGTCCG GATCTGGGCA GAGTATGACA GAGCAGCGTG TGGCCGAATC
    CATTACACTG AGATGTATGA AATGCTGACT CTTATGTCAC CACCGCTAGG CCTCGGCAAG
    AGATGTCCTT CCAAAGTGGC CTATAAGAGA CTGGTGCTGA TGAACATGCC CGTGGCCGAG
    GACATGACGG TCCATTTCAC CTCCACCCTG ATGGCGCTGA TACGCACCGC CCTGGACATC
    AAAATTGCCA AAGGTGGTGC AGATTGGCAG CAGTTAGACT CTGAGCTTCA AAAGGAGATC
    CTGACCATTT GGCCTCACCT GTCCCAGAAG ATGCTTGACC TGTTGGTACC CATGCCAAAA
    ACCTCTGACC TGACTGTTGG CAAAATATAT GCAGCCATGA TGATCATGGA TTACTACAAG
    CAGAGCAAGG CGAAGAAGCA GAGGCAGCAG CTGGAGGAGC AGAAAAATGC GCCCATGTTC
    CAGCGCATGG AGCCATCCTC TTTGCCACAG GAAATCATCT CCAACGCAAA GGCTCTGCCT
    TACCTCCAGC AGGAGACCCT CACAGGGCTG AGCAGCCGGA GCGGGTTCCC CTCGCTGAGC
    CCGCTCTCAC CACAGGAGAT CTTCCAGCTG GCGTGCATGG ATCCGGCCCA CGGGCAGTTC
    CAGGAGCACC AGTCTCTGGA GCCAGAGGTT AGGGAGTTTC AAAGAGTGCA GCCCTCTAAC
    CGTGGCAGCT ACCTTCCCGT GGACACTCAG GACCACGCTG TCTCTGGGAG GGCCTCCTCC
    ATGCCTCGTC TCACTGTGGA TCCCCAGGTG GTGACGGACA CCAGCTCGAT GCGGCGGTCA
    TTTTCCACCA TCCGGGACAA ACGCACCAAC ACCTCCTGGC TGGATGAGTT CTCCATGGAG
    CGCAGCAGCG ACAACACCTA CAAGTCCCGC CGGCGCAGCT ACCACTCCTC TCTCCAGCTC
    TCTGCCCGGC GCCTCAACGC TGACTCAGGC CACAGGTCTG AGGGGCACCG CTCGGGCGGC
    AGGGAGCGGG GCCGATCCAA AGAGCGCAAG CACTTGCTGT CCCCTGACAT CTCCCGCTGC
    AACTCAGAGG AGAGGAGTCC CCAGGCACAC GATGAGTCCC CAGAGCGGCG GCGCGAGTCC
    CGGTCACCCA GCGAGGGCAG GTCGCAAACG CCCAACCGGC AGGGAACGGG CTCCCTGAGC
    GAAAGCTCCA TCCCCTCCAT CTCGGACACC AGCACACCCC GGCGAGGCCG CCGCCAGCTC
    CCACCTGTGC CCCCAAAACC GCGTCCCCTC CTCTCCTACG CCTCCATGCT GCGGCACGCT
    GCCGGAGACG CCTCACCACC TCCCGATGAG ACCGAAGGGG GGTCCCCGCT CCTCTCCAAG
    GAGCCCAACG CCACCGGCCT GACCGAGTCT TCCAGCTCCC CGGCGGGGAA GCCGAGCCGG
    CCGTCCACCC CGCAGCGTTA CATCTCGGAG CCCTACCTGG CCCTGCACGA CGACTCGCAC
    GCTTCGGACT GCGGCGAGGA GGAGACGCTC ACCTTCGAGG CGGCCGTGGC CACCAGTCTG
    GGTCGTTCCA ACACCATCGG CTCGGCCCCA CCGCTGCGAC ACAGCTGGCA GATGCCCAAC
    GGGCACTACC GGAGACGGAG GCGAGGGGCC GGGCAGGGCG TGATGTGCGG GGCCAGCATC
    GATGTGCTCA GCGACACCGA GGAAGATGAC AAGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152516
    Clone ID Related Accession (Same CDS sequence) XM_015290487.2
    Accession Version XM_015290487.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290487.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAGC GGGAGCGGCG CCGGAGGCAC
    CACATGTCGG TGTGGGAGCA GCGCACCAGC CAGCTGCGCC GGCACATGCA GATGTCCAGC
    CAGGAGGGCA TCAATAAGGA TGAGCCACCC CTCATCAACC CACATGCCAG CATCTTCCGC
    CGCAAGAAGC CGGGGGACGG TGTTGCCTTG GAGAAGTGCG ATGAAGAGCA AGGCGGCAAG
    GGTGAGCGGC CGCCAGCTGA GGGTCCCGAG CAGCCGGCCC CAGGGACCAA CCCAGGCGGT
    GGGGAGGACA GGAAGAGCCC ATCGCCCCGT GCCAGGAGGG ACAAGGAGAT GTGGCAACAC
    AAAGCGTGCC ACGGGAACTG CGAGCCGGGT GAGGAAGGGG CAGGAGGTGG CATCGAGGAG
    AGGGCGCGGA TGAGACAGAG CCAGCGGCGC AGCCGGCATC GGAAAGCCAG GATGGAGGGG
    AAGGAGACGA CCGGAGCCCT GGAGAGCCGT GCGGGCAGCC AGGAGATGGG GCTGGAGGAG
    CCCTGCCCCG CAGAGGGGAT GCAGGATGGG GAGCGGCGTG GGGATTCTGC TGCCGCGCTG
    GACCTGATTC AGGGCGAGCT GGAGGCGAGC GGGGACCCCA CCAGGACTAA TGGGGTTCCC
    GCCGAGGAGG GAAGCCTCCC AGCATCAGCC CCTGAGCCTT CCCGGGGGAT GGAGGGCAGC
    CTGGGTGAGC AGGACTGCAG CAGCCCAGAC ACCAGTGAGC AGGCCCTGCT GGGGGGGGCG
    GCACTGGGGG CCAGCCGCAC CGTCAGCCAC AGCGAGCCTG ACCTGTCCTC CGTCACCGCC
    AACACTGAGA AGGCCACCGA GAGCACCACC ATCATGATTG ATGTCCAAGA CTCCACTGTG
    GTACAGATTA GCAACAAGAC TGATGGAGAA GCCAGCCCCT TGAAGGAGGC AGAGAGCAAA
    GAGGATGAGG AAGAGATGGA GAAGAAGAAG CGCAAGAAGG AGAAGAGCGA GACGGGCAAA
    GCGATGGTGC CACACAGCTC CATGTTCATC TTCAGCACCA CAAACCCGGT GCGCAGAGCC
    TGCCACTACA TTGTGAACCT GCGCTACTTT GAGATGTGCA TCCTCCTGGT CATCGCCGCC
    AGCAGCATCG CCCTCGCGGC CGAGGATCCC GTCCTCACCA ACTCCGACCG TAACAAAGTG
    CTGAGGTATT TTGATTACGT TTTCACCGGT GTCTTCACCT TCGAGATGGT TATCAAGATG
    ATCGACCAGG GCTTGATCCT GCAGGATGGC TCCTACTTCC GAGACCTCTG GAACATCCTG
    GATTTCATCG TGGTGGTGGG AGCTCTGGTG GCCTTTGCCT TGGCGAATGC TTTGGGGACC
    AACAAGGGTC GGGATATCAA GACTATCAAG TCTCTCCGTG TGCTGCGGGT CTTGAGGCCC
    CTGAAAACCA TCAAGCGGCT GCCGAAGCTC AAGGCTGTCT TTGACTGTGT TGTGACATCC
    CTCAAGAACG TCTTCAACAT CCTCATTGTC TACAAGCTCT TCATGTTCAT CTTTGCTGTC
    ATCGCCGTCC AGCTCTTCAA GGGGAAGTTT TTCTACTGCA CTGACAGCTC TAAGGACACA
    GAGAAGGACT GCATAGGGAA CTATGTGGAC CATGAGAAGA ACAAGATGGA GGTGAAGTGC
    CGGGAGTGGA AACGACATGA GTTTCACTAT GACAATATAA TCTGGGCGTT GCTCACCCTG
    TTCACAGTCT CCACCGGCGA GGGTTGGCCC CAGGTGCTGC AGCATTCGGT GGATGTGACG
    GAGGAGGACC GTGGGCCCAG CCGCAGTAAC CGCATGGAGA TGTCCATTTT TTACGTTGTC
    TATTTTGTGG TCTTCCCTTT CTTCTTCGTC AACATTTTTG TGGCTCTCAT CATCATCACA
    TTCCAAGAGC AAGGAGACAA AATGATGGAG GAGTGCAGTC TGGAGAAGAA CGAGAGGGCC
    TGTATTGACT TTGCCATCAG TGCCAAGCCC CTCACACGCT ACATGCCCCA GAACCGGCAC
    ACCTTCCAGT ATCGGGTCTG GCACTTTGTG GTCTCTCCAT CCTTTGAGTA TACCATCATG
    GCCATGATTG CTTTGAACAC TGTGGTGCTG ATGATGAAGT ACTACTCTGC TCCATACACC
    TATGAGCTGG CCCTGAAGTA CCTGAACATC GCTTTCACCA TGGTGTTCTC TTTGGAGTGC
    GTCCTTAAGA TCATTGCTTT TGGCTTCCTG AATTATTTCC GGGATACCTG GAATATCTTT
    GACTTCATCA CCGTCATCGG CAGCATCACA GAGATCATCC TGACAGACAC CAAGCTGGTG
    AACACCAGTA GCTTCAACAT GAGTTTCCTG AAACTCTTCC GGGCGGCTCG GCTCATCAAG
    CTGCTGCGCC AGGGTTACAC CATCCGCATC CTGCTGTGGA CGTTCGTGCA GTCCTTCAAG
    GCTCTCCCCT ATGTCTGCCT CCTAATCGCG ATGCTTTTCT TCATCTATGC CATCATTGGG
    ATGCAGGTTT TCGGCAACAT CAAACTGGAT GAGGAAAGCC ACATTAACCG GCACAACAAC
    TTCCGCAGCT TCCTGGGCTC CCTCATGCTC CTCTTCAGGA GTGCCACCGG TGAGGCATGG
    CAGGAGATCA TGCTCTCCTG CCTGGAGGGC AAGGGCTGCG AGCCGGACAC GACAGCGACG
    TCCGGGCAGA ATGAGAACGA GCGCTGCGGC ACCGACCTGG CCTACGTCTA CTTTGTCTCC
    TTCATCTTCT TCTGCTCATT CCTGATGCTC AACCTGTTCG TGGCGGTCAT CATGGACAAT
    TTTGAGTACC TGACTCGAGA TTCCTCCATC CTGGGACCTC ACCATCTAGA TGAATTTGTC
    CGGATCTGGG CAGAGTATGA CAGAGCAGCG TGTGGCCGAA TCCATTACAC TGAGATGTAT
    GAAATGCTGA CTCTTATGTC ACCACCGCTA GGCCTCGGCA AGAGATGTCC TTCCAAAGTG
    GCCTATAAGA GACTGGTGCT GATGAACATG CCCGTGGCCG AGGACATGAC GGTCCATTTC
    ACCTCCACCC TGATGGCGCT GATACGCACC GCCCTGGACA TCAAAATTGC CAAAGGTGGT
    GCAGATTGGC AGCAGTTAGA CTCTGAGCTT CAAAAGGAGA TCCTGACCAT TTGGCCTCAC
    CTGTCCCAGA AGATGCTTGA CCTGTTGGTA CCCATGCCAA AAACCTCTGA CCTGACTGTT
    GGCAAAATAT ATGCAGCCAT GATGATCATG GATTACTACA AGCAGAGCAA GGCGAAGAAG
    CAGAGGCAGC AGCTGGAGGA GCAGAAAAAT GCGCCCATGT TCCAGCGCAT GGAGCCATCC
    TCTTTGCCAC AGGAAATCAT CTCCAACGCA AAGGCTCTGC CTTACCTCCA GCAGGAGACC
    CTCACAGGGC TGAGCAGCCG GAGCGGGTTC CCCTCGCTGA GCCCGCTCTC ACCACAGGAG
    ATCTTCCAGC TGGCGTGCAT GGATCCGGCC CACGGGCAGT TCCAGGAGCA CCAGTCTCTG
    GTGGTGACGG ACACCAGCTC GATGCGGCGG TCATTTTCCA CCATCCGGGA CAAACGCACC
    AACACCTCCT GGCTGGATGA GTTCTCCATG GAGCGCAGCA GCGACAACAC CTACAAGTCC
    CGCCGGCGCA GCTACCACTC CTCTCTCCAG CTCTCTGCCC GGCGCCTCAA CGCTGACTCA
    GGCCACAGGT CTGAGGGGCA CCGCTCGGGC GGCAGGGAGC GGGGCCGATC CAAAGAGCGC
    AAGCACTTGC TGTCCCCTGA CATCTCCCGC TGCAACTCAG AGGAGAGGAG TCCCCAGGCA
    CACGATGAGT CCCCAGAGCG GCGGCGCGAG TCCCGGTCAC CCAGCGAGGG CAGGTCGCAA
    ACGCCCAACC GGCAGGGAAC GGGCTCCCTG AGCGAAAGCT CCATCCCCTC CATCTCGGAC
    ACCAGCACAC CCCGGCGAGG CCGCCGCCAG CTCCCACCTG TGCCCCCAAA ACCGCGTCCC
    CTCCTCTCCT ACGCCTCCAT GCTGCGGCAC GCTGCCGGAG ACGCCTCACC ACCTCCCGAT
    GAGACCGAAG GGGGGTCCCC GCTCCTCTCC AAGGAGCCCA ACGCCACCGG CCTGACCGAG
    TCTTCCAGCT CCCCGGCGGG GAAGCCGAGC CGGCCGTCCA CCCCGCAGCG TTACATCTCG
    GAGCCCTACC TGGCCCTGCA CGACGACTCG CACGCTTCGG ACTGCGGCGA GGAGGAGACG
    CTCACCTTCG AGGCGGCCGT GGCCACCAGT CTGGGTCGTT CCAACACCAT CGGCTCGGCC
    CCACCGCTGC GACACAGCTG GCAGATGCCC AACGGGCACT ACCGGAGACG GAGGCGAGGG
    GCCGGGCAGG GCGTGATGTG CGGGGCCAGC ATCGATGTGC TCAGCGACAC CGAGGAAGAT
    GACAAGTGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145973.2
    CDS127..7218
    Translation

    Target ORF information:

    RefSeq Version XM_015290487.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290487.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAGC GGGAGCGGCG CCGGAGGCAC
    CACATGTCGG TGTGGGAGCA GCGCACCAGC CAGCTGCGCC GGCACATGCA GATGTCCAGC
    CAGGAGGGCA TCAATAAGGA TGAGCCACCC CTCATCAACC CACATGCCAG CATCTTCCGC
    CGCAAGAAGC CGGGGGACGG TGTTGCCTTG GAGAAGTGCG ATGAAGAGCA AGGCGGCAAG
    GGTGAGCGGC CGCCAGCTGA GGGTCCCGAG CAGCCGGCCC CAGGGACCAA CCCAGGCGGT
    GGGGAGGACA GGAAGAGCCC ATCGCCCCGT GCCAGGAGGG ACAAGGAGAT GTGGCAACAC
    AAAGCGTGCC ACGGGAACTG CGAGCCGGGT GAGGAAGGGG CAGGAGGTGG CATCGAGGAG
    AGGGCGCGGA TGAGACAGAG CCAGCGGCGC AGCCGGCATC GGAAAGCCAG GATGGAGGGG
    AAGGAGACGA CCGGAGCCCT GGAGAGCCGT GCGGGCAGCC AGGAGATGGG GCTGGAGGAG
    CCCTGCCCCG CAGAGGGGAT GCAGGATGGG GAGCGGCGTG GGGATTCTGC TGCCGCGCTG
    GACCTGATTC AGGGCGAGCT GGAGGCGAGC GGGGACCCCA CCAGGACTAA TGGGGTTCCC
    GCCGAGGAGG GAAGCCTCCC AGCATCAGCC CCTGAGCCTT CCCGGGGGAT GGAGGGCAGC
    CTGGGTGAGC AGGACTGCAG CAGCCCAGAC ACCAGTGAGC AGGCCCTGCT GGGGGGGGCG
    GCACTGGGGG CCAGCCGCAC CGTCAGCCAC AGCGAGCCTG ACCTGTCCTC CGTCACCGCC
    AACACTGAGA AGGCCACCGA GAGCACCACC ATCATGATTG ATGTCCAAGA CTCCACTGTG
    GTACAGATTA GCAACAAGAC TGATGGAGAA GCCAGCCCCT TGAAGGAGGC AGAGAGCAAA
    GAGGATGAGG AAGAGATGGA GAAGAAGAAG CGCAAGAAGG AGAAGAGCGA GACGGGCAAA
    GCGATGGTGC CACACAGCTC CATGTTCATC TTCAGCACCA CAAACCCGGT GCGCAGAGCC
    TGCCACTACA TTGTGAACCT GCGCTACTTT GAGATGTGCA TCCTCCTGGT CATCGCCGCC
    AGCAGCATCG CCCTCGCGGC CGAGGATCCC GTCCTCACCA ACTCCGACCG TAACAAAGTG
    CTGAGGTATT TTGATTACGT TTTCACCGGT GTCTTCACCT TCGAGATGGT TATCAAGATG
    ATCGACCAGG GCTTGATCCT GCAGGATGGC TCCTACTTCC GAGACCTCTG GAACATCCTG
    GATTTCATCG TGGTGGTGGG AGCTCTGGTG GCCTTTGCCT TGGCGAATGC TTTGGGGACC
    AACAAGGGTC GGGATATCAA GACTATCAAG TCTCTCCGTG TGCTGCGGGT CTTGAGGCCC
    CTGAAAACCA TCAAGCGGCT GCCGAAGCTC AAGGCTGTCT TTGACTGTGT TGTGACATCC
    CTCAAGAACG TCTTCAACAT CCTCATTGTC TACAAGCTCT TCATGTTCAT CTTTGCTGTC
    ATCGCCGTCC AGCTCTTCAA GGGGAAGTTT TTCTACTGCA CTGACAGCTC TAAGGACACA
    GAGAAGGACT GCATAGGGAA CTATGTGGAC CATGAGAAGA ACAAGATGGA GGTGAAGTGC
    CGGGAGTGGA AACGACATGA GTTTCACTAT GACAATATAA TCTGGGCGTT GCTCACCCTG
    TTCACAGTCT CCACCGGCGA GGGTTGGCCC CAGGTGCTGC AGCATTCGGT GGATGTGACG
    GAGGAGGACC GTGGGCCCAG CCGCAGTAAC CGCATGGAGA TGTCCATTTT TTACGTTGTC
    TATTTTGTGG TCTTCCCTTT CTTCTTCGTC AACATTTTTG TGGCTCTCAT CATCATCACA
    TTCCAAGAGC AAGGAGACAA AATGATGGAG GAGTGCAGTC TGGAGAAGAA CGAGAGGGCC
    TGTATTGACT TTGCCATCAG TGCCAAGCCC CTCACACGCT ACATGCCCCA GAACCGGCAC
    ACCTTCCAGT ATCGGGTCTG GCACTTTGTG GTCTCTCCAT CCTTTGAGTA TACCATCATG
    GCCATGATTG CTTTGAACAC TGTGGTGCTG ATGATGAAGT ACTACTCTGC TCCATACACC
    TATGAGCTGG CCCTGAAGTA CCTGAACATC GCTTTCACCA TGGTGTTCTC TTTGGAGTGC
    GTCCTTAAGA TCATTGCTTT TGGCTTCCTG AATTATTTCC GGGATACCTG GAATATCTTT
    GACTTCATCA CCGTCATCGG CAGCATCACA GAGATCATCC TGACAGACAC CAAGCTGGTG
    AACACCAGTA GCTTCAACAT GAGTTTCCTG AAACTCTTCC GGGCGGCTCG GCTCATCAAG
    CTGCTGCGCC AGGGTTACAC CATCCGCATC CTGCTGTGGA CGTTCGTGCA GTCCTTCAAG
    GCTCTCCCCT ATGTCTGCCT CCTAATCGCG ATGCTTTTCT TCATCTATGC CATCATTGGG
    ATGCAGGTTT TCGGCAACAT CAAACTGGAT GAGGAAAGCC ACATTAACCG GCACAACAAC
    TTCCGCAGCT TCCTGGGCTC CCTCATGCTC CTCTTCAGGA GTGCCACCGG TGAGGCATGG
    CAGGAGATCA TGCTCTCCTG CCTGGAGGGC AAGGGCTGCG AGCCGGACAC GACAGCGACG
    TCCGGGCAGA ATGAGAACGA GCGCTGCGGC ACCGACCTGG CCTACGTCTA CTTTGTCTCC
    TTCATCTTCT TCTGCTCATT CCTGATGCTC AACCTGTTCG TGGCGGTCAT CATGGACAAT
    TTTGAGTACC TGACTCGAGA TTCCTCCATC CTGGGACCTC ACCATCTAGA TGAATTTGTC
    CGGATCTGGG CAGAGTATGA CAGAGCAGCG TGTGGCCGAA TCCATTACAC TGAGATGTAT
    GAAATGCTGA CTCTTATGTC ACCACCGCTA GGCCTCGGCA AGAGATGTCC TTCCAAAGTG
    GCCTATAAGA GACTGGTGCT GATGAACATG CCCGTGGCCG AGGACATGAC GGTCCATTTC
    ACCTCCACCC TGATGGCGCT GATACGCACC GCCCTGGACA TCAAAATTGC CAAAGGTGGT
    GCAGATTGGC AGCAGTTAGA CTCTGAGCTT CAAAAGGAGA TCCTGACCAT TTGGCCTCAC
    CTGTCCCAGA AGATGCTTGA CCTGTTGGTA CCCATGCCAA AAACCTCTGA CCTGACTGTT
    GGCAAAATAT ATGCAGCCAT GATGATCATG GATTACTACA AGCAGAGCAA GGCGAAGAAG
    CAGAGGCAGC AGCTGGAGGA GCAGAAAAAT GCGCCCATGT TCCAGCGCAT GGAGCCATCC
    TCTTTGCCAC AGGAAATCAT CTCCAACGCA AAGGCTCTGC CTTACCTCCA GCAGGAGACC
    CTCACAGGGC TGAGCAGCCG GAGCGGGTTC CCCTCGCTGA GCCCGCTCTC ACCACAGGAG
    ATCTTCCAGC TGGCGTGCAT GGATCCGGCC CACGGGCAGT TCCAGGAGCA CCAGTCTCTG
    GTGGTGACGG ACACCAGCTC GATGCGGCGG TCATTTTCCA CCATCCGGGA CAAACGCACC
    AACACCTCCT GGCTGGATGA GTTCTCCATG GAGCGCAGCA GCGACAACAC CTACAAGTCC
    CGCCGGCGCA GCTACCACTC CTCTCTCCAG CTCTCTGCCC GGCGCCTCAA CGCTGACTCA
    GGCCACAGGT CTGAGGGGCA CCGCTCGGGC GGCAGGGAGC GGGGCCGATC CAAAGAGCGC
    AAGCACTTGC TGTCCCCTGA CATCTCCCGC TGCAACTCAG AGGAGAGGAG TCCCCAGGCA
    CACGATGAGT CCCCAGAGCG GCGGCGCGAG TCCCGGTCAC CCAGCGAGGG CAGGTCGCAA
    ACGCCCAACC GGCAGGGAAC GGGCTCCCTG AGCGAAAGCT CCATCCCCTC CATCTCGGAC
    ACCAGCACAC CCCGGCGAGG CCGCCGCCAG CTCCCACCTG TGCCCCCAAA ACCGCGTCCC
    CTCCTCTCCT ACGCCTCCAT GCTGCGGCAC GCTGCCGGAG ACGCCTCACC ACCTCCCGAT
    GAGACCGAAG GGGGGTCCCC GCTCCTCTCC AAGGAGCCCA ACGCCACCGG CCTGACCGAG
    TCTTCCAGCT CCCCGGCGGG GAAGCCGAGC CGGCCGTCCA CCCCGCAGCG TTACATCTCG
    GAGCCCTACC TGGCCCTGCA CGACGACTCG CACGCTTCGG ACTGCGGCGA GGAGGAGACG
    CTCACCTTCG AGGCGGCCGT GGCCACCAGT CTGGGTCGTT CCAACACCAT CGGCTCGGCC
    CCACCGCTGC GACACAGCTG GCAGATGCCC AACGGGCACT ACCGGAGACG GAGGCGAGGG
    GCCGGGCAGG GCGTGATGTG CGGGGCCAGC ATCGATGTGC TCAGCGACAC CGAGGAAGAT
    GACAAGTGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152517
    Clone ID Related Accession (Same CDS sequence) XM_015290488.2
    Accession Version XM_015290488.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290488.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAGC GGGAGCGGCG CCGGAGGCAC
    CACATGTCGG TGTGGGAGCA GCGCACCAGC CAGCTGCGCC GGCACATGCA GATGTCCAGC
    CAGGAGGGCA TCAATAAGGA TGAGCCACCC CTCATCAACC CACATGCCAG CATCTTCCGC
    CGCAAGAAGC CGGGGGACGG TGTTGCCTTG GAGAAGTGCG ATGAAGAGCA AGGCGGCAAG
    GGTGAGCGGC CGCCAGCTGA GGGTCCCGAG CAGCCGGCCC CAGGGACCAA CCCAGGCGGT
    GGGGAGGACA GGAAGAGCCC ATCGCCCCGT GCCAGGAGGG ACAAGGAGAT GTGGCAACAC
    AAAGCGTGCC ACGGGAACTG CGAGCCGGGT GAGGAAGGGG CAGGAGGTGG CATCGAGGAG
    AGGGCGCGGA TGAGACAGAG CCAGCGGCGC AGCCGGCATC GGAAAGCCAG GATGGAGGGG
    AAGGAGACGA CCGGAGCCCT GGAGAGCCGT GCGGGCAGCC AGGAGATGGG GCTGGAGGAG
    CCCTGCCCCG CAGAGGGGAT GCAGGATGGG GAGCGGCGTG GGGATTCTGC TGCCGCGCTG
    GACCTGATTC AGGGCGAGCT GGAGGCGAGC GGGGACCCCA CCAGGACTAA TGGGGTTCCC
    GCCGAGGAGG GAAGCCTCCC AGCATCAGCC CCTGAGCCTT CCCGGGGGAT GGAGGGCAGC
    CTGGGTGAGC AGGACTGCAG CAGCCCAGAC ACCAGTGAGC AGGCCCTGCT GGGGGGGGCG
    GCACTGGGGG CCAGCCGCAC CGTCAGCCAC AGCGAGCCTG ACCTGTCCTC CGTCACCGCC
    AACACTGAGA AGGCCACCGA GAGCACCACC ATCATGATTG ATGTCCAAGA CTCCACTGTG
    GTACAGATTA GCAACAAGAC TGATGGAGAA GCCAGCCCCT TGAAGGAGGC AGAGAGCAAA
    GAGGATGAGG AAGAGATGGA GAAGAAGAAG CGCAAGAAGG AGAAGAGCGA GACGGGCAAA
    GCGATGGTGC CACACAGCTC CATGTTCATC TTCAGCACCA CAAACCCGGT GCGCAGAGCC
    TGCCACTACA TTGTGAACCT GCGCTACTTT GAGATGTGCA TCCTCCTGGT CATCGCCGCC
    AGCAGCATCG CCCTCGCGGC CGAGGATCCC GTCCTCACCA ACTCCGACCG TAACAAAGTG
    CTGAGGTATT TTGATTACGT TTTCACCGGT GTCTTCACCT TCGAGATGGT TATCAAGATG
    ATCGACCAGG GCTTGATCCT GCAGGATGGC TCCTACTTCC GAGACCTCTG GAACATCCTG
    GATTTCATCG TGGTGGTGGG AGCTCTGGTG GCCTTTGCCT TGGCGAATGC TTTGGGGACC
    AACAAGGGTC GGGATATCAA GACTATCAAG TCTCTCCGTG TGCTGCGGGT CTTGAGGCCC
    CTGAAAACCA TCAAGCGGCT GCCGAAGCTC AAGGCTGTCT TTGACTGTGT TGTGACATCC
    CTCAAGAACG TCTTCAACAT CCTCATTGTC TACAAGCTCT TCATGTTCAT CTTTGCTGTC
    ATCGCCGTCC AGCTCTTCAA GGGGAAGTTT TTCTACTGCA CTGACAGCTC TAAGGACACA
    GAGAAGGACT GCATAGGGAA CTATGTGGAC CATGAGAAGA ACAAGATGGA GGTGAAGTGC
    CGGGAGTGGA AACGACATGA GTTTCACTAT GACAATATAA TCTGGGCGTT GCTCACCCTG
    TTCACAGTCT CCACCGGCGA GGGTTGGCCC CAGGTGCTGC AGCATTCGGT GGATGTGACG
    GAGGAGGACC GTGGGCCCAG CCGCAGTAAC CGCATGGAGA TGTCCATTTT TTACGTTGTC
    TATTTTGTGG TCTTCCCTTT CTTCTTCGTC AACATTTTTG TGGCTCTCAT CATCATCACA
    TTCCAAGAGC AAGGAGACAA AATGATGGAG GAGTGCAGTC TGGAGAAGAA CGAGAGGGCC
    TGTATTGACT TTGCCATCAG TGCCAAGCCC CTCACACGCT ACATGCCCCA GAACCGGCAC
    ACCTTCCAGT ATCGGGTCTG GCACTTTGTG GTCTCTCCAT CCTTTGAGTA TACCATCATG
    GCCATGATTG CTTTGAACAC TGTGGTGCTG ATGATGAAGT ACTACTCTGC TCCATACACC
    TATGAGCTGG CCCTGAAGTA CCTGAACATC GCTTTCACCA TGGTGTTCTC TTTGGAGTGC
    GTCCTTAAGA TCATTGCTTT TGGCTTCCTG AATTATTTCC GGGATACCTG GAATATCTTT
    GACTTCATCA CCGTCATCGG CAGCATCACA GAGATCATCC TGACAGACAC CAAGCTGGTG
    AACACCAGTA GCTTCAACAT GAGTTTCCTG AAACTCTTCC GGGCGGCTCG GCTCATCAAG
    CTGCTGCGCC AGGGTTACAC CATCCGCATC CTGCTGTGGA CGTTCGTGCA GTCCTTCAAG
    GCTCTCCCCT ATGTCTGCCT CCTAATCGCG ATGCTTTTCT TCATCTATGC CATCATTGGG
    ATGCAGGTTT TCGGCAACAT CAAACTGGAT GAGGAAAGCC ACATTAACCG GCACAACAAC
    TTCCGCAGCT TCCTGGGCTC CCTCATGCTC CTCTTCAGGA GTGCCACCGG TGAGGCATGG
    CAGGAGATCA TGCTCTCCTG CCTGGAGGGC AAGGGCTGCG AGCCGGACAC GACAGCGACG
    TCCGGGCAGA ATGAGAACGA GCGCTGCGGC ACCGACCTGG CCTACGTCTA CTTTGTCTCC
    TTCATCTTCT TCTGCTCATT CCTGATGCTC AACCTGTTCG TGGCGGTCAT CATGGACAAT
    TTTGAGTACC TGACTCGAGA TTCCTCCATC CTGGGACCTC ACCATCTAGA TGAATTTGTC
    CGGATCTGGG CAGAGTATGA CAGAGCAGCG TGCGGCAGGA TCTCGTATAA GGATATGTAC
    AAATTAGTAC GGGTGATCTC GCCTCCTCTG GGCCTAGGAG AGAACTGCCC CTACAGGGTG
    GCGTGCAAGA GACTGGTGCT GATGAACATG CCCGTGGCCG AGGACATGAC GGTCCATTTC
    ACCTCCACCC TGATGGCGCT GATACGCACC GCCCTGGACA TCAAAATTGC CAAAGGTGGT
    GCAGATTGGC AGCAGTTAGA CTCTGAGCTT CAAAAGGAGA TCCTGACCAT TTGGCCTCAC
    CTGTCCCAGA AGATGCTTGA CCTGTTGGTA CCCATGCCAA AAACCTCTGA CCTGACTGTT
    GGCAAAATAT ATGCAGCCAT GATGATCATG GATTACTACA AGCAGAGCAA GGCGAAGAAG
    CAGAGGCAGC AGCTGGAGGA GCAGAAAAAT GCGCCCATGT TCCAGCGCAT GGAGCCATCC
    TCTTTGCCAC AGGAAATCAT CTCCAACGCA AAGGCTCTGC CTTACCTCCA GCAGGAGACC
    CTCACAGGGC TGAGCAGCCG GAGCGGGTTC CCCTCGCTGA GCCCGCTCTC ACCACAGGAG
    ATCTTCCAGC TGGCGTGCAT GGATCCGGCC CACGGGCAGT TCCAGGAGCA CCAGTCTCTG
    GTGGTGACGG ACACCAGCTC GATGCGGCGG TCATTTTCCA CCATCCGGGA CAAACGCACC
    AACACCTCCT GGCTGGATGA GTTCTCCATG GAGCGCAGCA GCGACAACAC CTACAAGTCC
    CGCCGGCGCA GCTACCACTC CTCTCTCCAG CTCTCTGCCC GGCGCCTCAA CGCTGACTCA
    GGCCACAGGT CTGAGGGGCA CCGCTCGGGC GGCAGGGAGC GGGGCCGATC CAAAGAGCGC
    AAGCACTTGC TGTCCCCTGA CATCTCCCGC TGCAACTCAG AGGAGAGGAG TCCCCAGGCA
    CACGATGAGT CCCCAGAGCG GCGGCGCGAG TCCCGGTCAC CCAGCGAGGG CAGGTCGCAA
    ACGCCCAACC GGCAGGGAAC GGGCTCCCTG AGCGAAAGCT CCATCCCCTC CATCTCGGAC
    ACCAGCACAC CCCGGCGAGG CCGCCGCCAG CTCCCACCTG TGCCCCCAAA ACCGCGTCCC
    CTCCTCTCCT ACGCCTCCAT GCTGCGGCAC GCTGCCGGAG ACGCCTCACC ACCTCCCGAT
    GAGACCGAAG GGGGGTCCCC GCTCCTCTCC AAGGAGCCCA ACGCCACCGG CCTGACCGAG
    TCTTCCAGCT CCCCGGCGGG GAAGCCGAGC CGGCCGTCCA CCCCGCAGCG TTACATCTCG
    GAGCCCTACC TGGCCCTGCA CGACGACTCG CACGCTTCGG ACTGCGGCGA GGAGGAGACG
    CTCACCTTCG AGGCGGCCGT GGCCACCAGT CTGGGTCGTT CCAACACCAT CGGCTCGGCC
    CCACCGCTGC GACACAGCTG GCAGATGCCC AACGGGCACT ACCGGAGACG GAGGCGAGGG
    GCCGGGCAGG GCGTGATGTG CGGGGCCAGC ATCGATGTGC TCAGCGACAC CGAGGAAGAT
    GACAAGTGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145974.2
    CDS127..7218
    Translation

    Target ORF information:

    RefSeq Version XM_015290488.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290488.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAGC GGGAGCGGCG CCGGAGGCAC
    CACATGTCGG TGTGGGAGCA GCGCACCAGC CAGCTGCGCC GGCACATGCA GATGTCCAGC
    CAGGAGGGCA TCAATAAGGA TGAGCCACCC CTCATCAACC CACATGCCAG CATCTTCCGC
    CGCAAGAAGC CGGGGGACGG TGTTGCCTTG GAGAAGTGCG ATGAAGAGCA AGGCGGCAAG
    GGTGAGCGGC CGCCAGCTGA GGGTCCCGAG CAGCCGGCCC CAGGGACCAA CCCAGGCGGT
    GGGGAGGACA GGAAGAGCCC ATCGCCCCGT GCCAGGAGGG ACAAGGAGAT GTGGCAACAC
    AAAGCGTGCC ACGGGAACTG CGAGCCGGGT GAGGAAGGGG CAGGAGGTGG CATCGAGGAG
    AGGGCGCGGA TGAGACAGAG CCAGCGGCGC AGCCGGCATC GGAAAGCCAG GATGGAGGGG
    AAGGAGACGA CCGGAGCCCT GGAGAGCCGT GCGGGCAGCC AGGAGATGGG GCTGGAGGAG
    CCCTGCCCCG CAGAGGGGAT GCAGGATGGG GAGCGGCGTG GGGATTCTGC TGCCGCGCTG
    GACCTGATTC AGGGCGAGCT GGAGGCGAGC GGGGACCCCA CCAGGACTAA TGGGGTTCCC
    GCCGAGGAGG GAAGCCTCCC AGCATCAGCC CCTGAGCCTT CCCGGGGGAT GGAGGGCAGC
    CTGGGTGAGC AGGACTGCAG CAGCCCAGAC ACCAGTGAGC AGGCCCTGCT GGGGGGGGCG
    GCACTGGGGG CCAGCCGCAC CGTCAGCCAC AGCGAGCCTG ACCTGTCCTC CGTCACCGCC
    AACACTGAGA AGGCCACCGA GAGCACCACC ATCATGATTG ATGTCCAAGA CTCCACTGTG
    GTACAGATTA GCAACAAGAC TGATGGAGAA GCCAGCCCCT TGAAGGAGGC AGAGAGCAAA
    GAGGATGAGG AAGAGATGGA GAAGAAGAAG CGCAAGAAGG AGAAGAGCGA GACGGGCAAA
    GCGATGGTGC CACACAGCTC CATGTTCATC TTCAGCACCA CAAACCCGGT GCGCAGAGCC
    TGCCACTACA TTGTGAACCT GCGCTACTTT GAGATGTGCA TCCTCCTGGT CATCGCCGCC
    AGCAGCATCG CCCTCGCGGC CGAGGATCCC GTCCTCACCA ACTCCGACCG TAACAAAGTG
    CTGAGGTATT TTGATTACGT TTTCACCGGT GTCTTCACCT TCGAGATGGT TATCAAGATG
    ATCGACCAGG GCTTGATCCT GCAGGATGGC TCCTACTTCC GAGACCTCTG GAACATCCTG
    GATTTCATCG TGGTGGTGGG AGCTCTGGTG GCCTTTGCCT TGGCGAATGC TTTGGGGACC
    AACAAGGGTC GGGATATCAA GACTATCAAG TCTCTCCGTG TGCTGCGGGT CTTGAGGCCC
    CTGAAAACCA TCAAGCGGCT GCCGAAGCTC AAGGCTGTCT TTGACTGTGT TGTGACATCC
    CTCAAGAACG TCTTCAACAT CCTCATTGTC TACAAGCTCT TCATGTTCAT CTTTGCTGTC
    ATCGCCGTCC AGCTCTTCAA GGGGAAGTTT TTCTACTGCA CTGACAGCTC TAAGGACACA
    GAGAAGGACT GCATAGGGAA CTATGTGGAC CATGAGAAGA ACAAGATGGA GGTGAAGTGC
    CGGGAGTGGA AACGACATGA GTTTCACTAT GACAATATAA TCTGGGCGTT GCTCACCCTG
    TTCACAGTCT CCACCGGCGA GGGTTGGCCC CAGGTGCTGC AGCATTCGGT GGATGTGACG
    GAGGAGGACC GTGGGCCCAG CCGCAGTAAC CGCATGGAGA TGTCCATTTT TTACGTTGTC
    TATTTTGTGG TCTTCCCTTT CTTCTTCGTC AACATTTTTG TGGCTCTCAT CATCATCACA
    TTCCAAGAGC AAGGAGACAA AATGATGGAG GAGTGCAGTC TGGAGAAGAA CGAGAGGGCC
    TGTATTGACT TTGCCATCAG TGCCAAGCCC CTCACACGCT ACATGCCCCA GAACCGGCAC
    ACCTTCCAGT ATCGGGTCTG GCACTTTGTG GTCTCTCCAT CCTTTGAGTA TACCATCATG
    GCCATGATTG CTTTGAACAC TGTGGTGCTG ATGATGAAGT ACTACTCTGC TCCATACACC
    TATGAGCTGG CCCTGAAGTA CCTGAACATC GCTTTCACCA TGGTGTTCTC TTTGGAGTGC
    GTCCTTAAGA TCATTGCTTT TGGCTTCCTG AATTATTTCC GGGATACCTG GAATATCTTT
    GACTTCATCA CCGTCATCGG CAGCATCACA GAGATCATCC TGACAGACAC CAAGCTGGTG
    AACACCAGTA GCTTCAACAT GAGTTTCCTG AAACTCTTCC GGGCGGCTCG GCTCATCAAG
    CTGCTGCGCC AGGGTTACAC CATCCGCATC CTGCTGTGGA CGTTCGTGCA GTCCTTCAAG
    GCTCTCCCCT ATGTCTGCCT CCTAATCGCG ATGCTTTTCT TCATCTATGC CATCATTGGG
    ATGCAGGTTT TCGGCAACAT CAAACTGGAT GAGGAAAGCC ACATTAACCG GCACAACAAC
    TTCCGCAGCT TCCTGGGCTC CCTCATGCTC CTCTTCAGGA GTGCCACCGG TGAGGCATGG
    CAGGAGATCA TGCTCTCCTG CCTGGAGGGC AAGGGCTGCG AGCCGGACAC GACAGCGACG
    TCCGGGCAGA ATGAGAACGA GCGCTGCGGC ACCGACCTGG CCTACGTCTA CTTTGTCTCC
    TTCATCTTCT TCTGCTCATT CCTGATGCTC AACCTGTTCG TGGCGGTCAT CATGGACAAT
    TTTGAGTACC TGACTCGAGA TTCCTCCATC CTGGGACCTC ACCATCTAGA TGAATTTGTC
    CGGATCTGGG CAGAGTATGA CAGAGCAGCG TGCGGCAGGA TCTCGTATAA GGATATGTAC
    AAATTAGTAC GGGTGATCTC GCCTCCTCTG GGCCTAGGAG AGAACTGCCC CTACAGGGTG
    GCGTGCAAGA GACTGGTGCT GATGAACATG CCCGTGGCCG AGGACATGAC GGTCCATTTC
    ACCTCCACCC TGATGGCGCT GATACGCACC GCCCTGGACA TCAAAATTGC CAAAGGTGGT
    GCAGATTGGC AGCAGTTAGA CTCTGAGCTT CAAAAGGAGA TCCTGACCAT TTGGCCTCAC
    CTGTCCCAGA AGATGCTTGA CCTGTTGGTA CCCATGCCAA AAACCTCTGA CCTGACTGTT
    GGCAAAATAT ATGCAGCCAT GATGATCATG GATTACTACA AGCAGAGCAA GGCGAAGAAG
    CAGAGGCAGC AGCTGGAGGA GCAGAAAAAT GCGCCCATGT TCCAGCGCAT GGAGCCATCC
    TCTTTGCCAC AGGAAATCAT CTCCAACGCA AAGGCTCTGC CTTACCTCCA GCAGGAGACC
    CTCACAGGGC TGAGCAGCCG GAGCGGGTTC CCCTCGCTGA GCCCGCTCTC ACCACAGGAG
    ATCTTCCAGC TGGCGTGCAT GGATCCGGCC CACGGGCAGT TCCAGGAGCA CCAGTCTCTG
    GTGGTGACGG ACACCAGCTC GATGCGGCGG TCATTTTCCA CCATCCGGGA CAAACGCACC
    AACACCTCCT GGCTGGATGA GTTCTCCATG GAGCGCAGCA GCGACAACAC CTACAAGTCC
    CGCCGGCGCA GCTACCACTC CTCTCTCCAG CTCTCTGCCC GGCGCCTCAA CGCTGACTCA
    GGCCACAGGT CTGAGGGGCA CCGCTCGGGC GGCAGGGAGC GGGGCCGATC CAAAGAGCGC
    AAGCACTTGC TGTCCCCTGA CATCTCCCGC TGCAACTCAG AGGAGAGGAG TCCCCAGGCA
    CACGATGAGT CCCCAGAGCG GCGGCGCGAG TCCCGGTCAC CCAGCGAGGG CAGGTCGCAA
    ACGCCCAACC GGCAGGGAAC GGGCTCCCTG AGCGAAAGCT CCATCCCCTC CATCTCGGAC
    ACCAGCACAC CCCGGCGAGG CCGCCGCCAG CTCCCACCTG TGCCCCCAAA ACCGCGTCCC
    CTCCTCTCCT ACGCCTCCAT GCTGCGGCAC GCTGCCGGAG ACGCCTCACC ACCTCCCGAT
    GAGACCGAAG GGGGGTCCCC GCTCCTCTCC AAGGAGCCCA ACGCCACCGG CCTGACCGAG
    TCTTCCAGCT CCCCGGCGGG GAAGCCGAGC CGGCCGTCCA CCCCGCAGCG TTACATCTCG
    GAGCCCTACC TGGCCCTGCA CGACGACTCG CACGCTTCGG ACTGCGGCGA GGAGGAGACG
    CTCACCTTCG AGGCGGCCGT GGCCACCAGT CTGGGTCGTT CCAACACCAT CGGCTCGGCC
    CCACCGCTGC GACACAGCTG GCAGATGCCC AACGGGCACT ACCGGAGACG GAGGCGAGGG
    GCCGGGCAGG GCGTGATGTG CGGGGCCAGC ATCGATGTGC TCAGCGACAC CGAGGAAGAT
    GACAAGTGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152518
    Clone ID Related Accession (Same CDS sequence) XM_025153110.1
    Accession Version XM_025153110.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAGC GGGAGCGGCG CCGGAGGCAC
    CACATGTCGG TGTGGGAGCA GCGCACCAGC CAGCTGCGCC GGCACATGCA GATGTCCAGC
    CAGGAGGGCA TCAATAAGGA TGAGCCACCC CTCATCAACC CACATGCCAG CATCTTCCGC
    CGCAAGAAGC CGGGGGACGG TGTTGCCTTG GAGAAGTGCG ATGAAGAGCA AGGCGGCAAG
    GGTGAGCGGC CGCCAGCTGA GGGTCCCGAG CAGCCGGCCC CAGGGACCAA CCCAGGCGGT
    GGGGAGGACA GGAAGAGCCC ATCGCCCCGT GCCAGGAGGG ACAAGGAGAT GTGGCAACAC
    AAAGCGTGCC ACGGGAACTG CGAGCCGGGT GAGGAAGGGG CAGGAGGTGG CATCGAGGAG
    AGGGCGCGGA TGAGACAGAG CCAGCGGCGC AGCCGGCATC GGAAAGCCAG GATGGAGGGG
    AAGGAGACGA CCGGAGCCCT GGAGAGCCGT GCGGGCAGCC AGGAGATGGG GCTGGAGGAG
    CCCTGCCCCG CAGAGGGGAT GCAGGATGGG GAGCGGCGTG GGGATTCTGC TGCCGCGCTG
    GACCTGATTC AGGGCGAGCT GGAGGCGAGC GGGGACCCCA CCAGGACTAA TGGGGTTCCC
    GCCGAGGAGG GAAGCCTCCC AGCATCAGCC CCTGAGCCTT CCCGGGGGAT GGAGGGCAGC
    CTGGGTGAGC AGGACTGCAG CAGCCCAGAC ACCAGTGAGC AGGCCCTGCT GGGGGGGGCG
    GCACTGGGGG CCAGCCGCAC CGTCAGCCAC AGCGAGCCTG ACCTGTCCTC CGTCACCGCC
    AACACTGAGA AGGCCACCGA GAGCACCACC ATCATGATTG ATGTCCAAGA CTCCACTGTG
    GTACAGATTA GCAACAAGAC TGATGGAGAA GCCAGCCCCT TGAAGGAGGC AGAGAGCAAA
    GAGGATGAGG AAGAGATGGA GAAGAAGAAG CGCAAGAAGG AGAAGAGCGA GACGGGCAAA
    GCGATGGTGC CACACAGCTC CATGTTCATC TTCAGCACCA CAAACCCGGT GCGCAGAGCC
    TGCCACTACA TTGTGAACCT GCGCTACTTT GAGATGTGCA TCCTCCTGGT CATCGCCGCC
    AGCAGCATCG CCCTCGCGGC CGAGGATCCC GTCCTCACCA ACTCCGACCG TAACAAAGTG
    CTGAGGTATT TTGATTACGT TTTCACCGGT GTCTTCACCT TCGAGATGGT TATCAAGATG
    ATCGACCAGG GCTTGATCCT GCAGGATGGC TCCTACTTCC GAGACCTCTG GAACATCCTG
    GATTTCATCG TGGTGGTGGG AGCTCTGGTG GCCTTTGCCT TGGCGACCAA CAAGGGTCGG
    GATATCAAGA CTATCAAGTC TCTCCGTGTG CTGCGGGTCT TGAGGCCCCT GAAAACCATC
    AAGCGGCTGC CGAAGCTCAA GGCTGTCTTT GACTGTGTTG TGACATCCCT CAAGAACGTC
    TTCAACATCC TCATTGTCTA CAAGCTCTTC ATGTTCATCT TTGCTGTCAT CGCCGTCCAG
    CTCTTCAAGG GGAAGTTTTT CTACTGCACT GACAGCTCTA AGGACACAGA GAAGGACTGC
    ATAGGGAACT ATGTGGACCA TGAGAAGAAC AAGATGGAGG TGAAGTGCCG GGAGTGGAAA
    CGACATGAGT TTCACTATGA CAATATAATC TGGGCGTTGC TCACCCTGTT CACAGTCTCC
    ACCGGCGAGG GTTGGCCCCA GGTGCTGCAG CATTCGGTGG ATGTGACGGA GGAGGACCGT
    GGGCCCAGCC GCAGTAACCG CATGGAGATG TCCATTTTTT ACGTTGTCTA TTTTGTGGTC
    TTCCCTTTCT TCTTCGTCAA CATTTTTGTG GCTCTCATCA TCATCACATT CCAAGAGCAA
    GGAGACAAAA TGATGGAGGA GTGCAGTCTG GAGAAGAACG AGAGGGCCTG TATTGACTTT
    GCCATCAGTG CCAAGCCCCT CACACGCTAC ATGCCCCAGA ACCGGCACAC CTTCCAGTAT
    CGGGTCTGGC ACTTTGTGGT CTCTCCATCC TTTGAGTATA CCATCATGGC CATGATTGCT
    TTGAACACTG TGGTGCTGAT GATGAAGTAC TACTCTGCTC CATACACCTA TGAGCTGGCC
    CTGAAGTACC TGAACATCGC TTTCACCATG GTGTTCTCTT TGGAGTGCGT CCTTAAGATC
    ATTGCTTTTG GCTTCCTGAA TTATTTCCGG GATACCTGGA ATATCTTTGA CTTCATCACC
    GTCATCGGCA GCATCACAGA GATCATCCTG ACAGACACCA AGCTGGTGAA CACCAGTAGC
    TTCAACATGA GTTTCCTGAA ACTCTTCCGG GCGGCTCGGC TCATCAAGCT GCTGCGCCAG
    GGTTACACCA TCCGCATCCT GCTGTGGACG TTCGTGCAGT CCTTCAAGGC TCTCCCCTAT
    GTCTGCCTCC TAATCGCGAT GCTTTTCTTC ATCTATGCCA TCATTGGGAT GCAGGTTTTC
    GGCAACATCA AACTGGATGA GGAAAGCCAC ATTAACCGGC ACAACAACTT CCGCAGCTTC
    CTGGGCTCCC TCATGCTCCT CTTCAGGAGT GCCACCGGTG AGGCATGGCA GGAGATCATG
    CTCTCCTGCC TGGAGGGCAA GGGCTGCGAG CCGGACACGA CAGCGACGTC CGGGCAGAAT
    GAGAACGAGC GCTGCGGCAC CGACCTGGCC TACGTCTACT TTGTCTCCTT CATCTTCTTC
    TGCTCATTCC TGATGCTCAA CCTGTTCGTG GCGGTCATCA TGGACAATTT TGAGTACCTG
    ACTCGAGATT CCTCCATCCT GGGACCTCAC CATCTAGATG AATTTGTCCG GATCTGGGCA
    GAGTATGACA GAGCAGCGTG TGGCCGAATC CATTACACTG AGATGTATGA AATGCTGACT
    CTTATGTCAC CACCGCTAGG CCTCGGCAAG AGATGTCCTT CCAAAGTGGC CTATAAGAGA
    CTGGTGCTGA TGAACATGCC CGTGGCCGAG GACATGACGG TCCATTTCAC CTCCACCCTG
    ATGGCGCTGA TACGCACCGC CCTGGACATC AAAATTGCCA AAGGTGGTGC AGATTGGCAG
    CAGTTAGACT CTGAGCTTCA AAAGGAGATC CTGACCATTT GGCCTCACCT GTCCCAGAAG
    ATGCTTGACC TGTTGGTACC CATGCCAAAA ACCTCTGACC TGACTGTTGG CAAAATATAT
    GCAGCCATGA TGATCATGGA TTACTACAAG CAGAGCAAGG CGAAGAAGCA GAGGCAGCAG
    CTGGAGGAGC AGAAAAATGC GCCCATGTTC CAGCGCATGG AGCCATCCTC TTTGCCACAG
    GAAATCATCT CCAACGCAAA GGCTCTGCCT TACCTCCAGC AGGAGACCCT CACAGGGCTG
    AGCAGCCGGA GCGGGTTCCC CTCGCTGAGC CCGCTCTCAC CACAGGAGAT CTTCCAGCTG
    GCGTGCATGG ATCCGGCCCA CGGGCAGTTC CAGGAGCACC AGTCTCTGGA GCCAGAGGTT
    AGGGAGTTTC AAAGAGTGCA GCCCTCTAAC CGTGGCAGCT ACCTTCCCGT GGACACTCAG
    GACCACGCTG TCTCTGGGAG GGCCTCCTCC ATGCCTCGTC TCACTGTGGA TCCCCAGGTG
    GTGACGGACA CCAGCTCGAT GCGGCGGTCA TTTTCCACCA TCCGGGACAA ACGCACCAAC
    ACCTCCTGGC TGGATGAGTT CTCCATGGAG CGCAGCAGCG ACAACACCTA CAAGTCCCGC
    CGGCGCAGCT ACCACTCCTC TCTCCAGCTC TCTGCCCGGC GCCTCAACGC TGACTCAGGC
    CACAGGTCTG AGGGGCACCG CTCGGGCGGC AGGGAGCGGG GCCGATCCAA AGAGCGCAAG
    CACTTGCTGT CCCCTGACAT CTCCCGCTGC AACTCAGAGG AGAGGAGTCC CCAGGCACAC
    GATGAGTCCC CAGAGCGGCG GCGCGAGTCC CGGTCACCCA GCGAGGGCAG GTCGCAAACG
    CCCAACCGGC AGGGAACGGG CTCCCTGAGC GAAAGCTCCA TCCCCTCCAT CTCGGACACC
    AGCACACCCC GGCGAGGCCG CCGCCAGCTC CCACCTGTGC CCCCAAAACC GCGTCCCCTC
    CTCTCCTACG CCTCCATGCT GCGGCACGCT GCCGGAGACG CCTCACCACC TCCCGATGAG
    ACCGAAGGGG GGTCCCCGCT CCTCTCCAAG GAGCCCAACG CCACCGGCCT GACCGAGTCT
    TCCAGCTCCC CGGCGGGGAA GCCGAGCCGG CCGTCCACCC CGCAGCGTTA CATCTCGGAG
    CCCTACCTGG CCCTGCACGA CGACTCGCAC GCTTCGGACT GCGGCGAGGA GGAGACGCTC
    ACCTTCGAGG CGGCCGTGGC CACCAGTCTG GGTCGTTCCA ACACCATCGG CTCGGCCCCA
    CCGCTGCGAC ACAGCTGGCA GATGCCCAAC GGGCACTACC GGAGACGGAG GCGAGGGGCC
    GGGCAGGGCG TGATGTGCGG GGCCAGCATC GATGTGCTCA GCGACACCGA GGAAGATGAC
    AAGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025008878.1
    CDS128..7336
    Translation

    Target ORF information:

    RefSeq Version XM_025153110.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153110.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTGTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAGC GGGAGCGGCG CCGGAGGCAC
    CACATGTCGG TGTGGGAGCA GCGCACCAGC CAGCTGCGCC GGCACATGCA GATGTCCAGC
    CAGGAGGGCA TCAATAAGGA TGAGCCACCC CTCATCAACC CACATGCCAG CATCTTCCGC
    CGCAAGAAGC CGGGGGACGG TGTTGCCTTG GAGAAGTGCG ATGAAGAGCA AGGCGGCAAG
    GGTGAGCGGC CGCCAGCTGA GGGTCCCGAG CAGCCGGCCC CAGGGACCAA CCCAGGCGGT
    GGGGAGGACA GGAAGAGCCC ATCGCCCCGT GCCAGGAGGG ACAAGGAGAT GTGGCAACAC
    AAAGCGTGCC ACGGGAACTG CGAGCCGGGT GAGGAAGGGG CAGGAGGTGG CATCGAGGAG
    AGGGCGCGGA TGAGACAGAG CCAGCGGCGC AGCCGGCATC GGAAAGCCAG GATGGAGGGG
    AAGGAGACGA CCGGAGCCCT GGAGAGCCGT GCGGGCAGCC AGGAGATGGG GCTGGAGGAG
    CCCTGCCCCG CAGAGGGGAT GCAGGATGGG GAGCGGCGTG GGGATTCTGC TGCCGCGCTG
    GACCTGATTC AGGGCGAGCT GGAGGCGAGC GGGGACCCCA CCAGGACTAA TGGGGTTCCC
    GCCGAGGAGG GAAGCCTCCC AGCATCAGCC CCTGAGCCTT CCCGGGGGAT GGAGGGCAGC
    CTGGGTGAGC AGGACTGCAG CAGCCCAGAC ACCAGTGAGC AGGCCCTGCT GGGGGGGGCG
    GCACTGGGGG CCAGCCGCAC CGTCAGCCAC AGCGAGCCTG ACCTGTCCTC CGTCACCGCC
    AACACTGAGA AGGCCACCGA GAGCACCACC ATCATGATTG ATGTCCAAGA CTCCACTGTG
    GTACAGATTA GCAACAAGAC TGATGGAGAA GCCAGCCCCT TGAAGGAGGC AGAGAGCAAA
    GAGGATGAGG AAGAGATGGA GAAGAAGAAG CGCAAGAAGG AGAAGAGCGA GACGGGCAAA
    GCGATGGTGC CACACAGCTC CATGTTCATC TTCAGCACCA CAAACCCGGT GCGCAGAGCC
    TGCCACTACA TTGTGAACCT GCGCTACTTT GAGATGTGCA TCCTCCTGGT CATCGCCGCC
    AGCAGCATCG CCCTCGCGGC CGAGGATCCC GTCCTCACCA ACTCCGACCG TAACAAAGTG
    CTGAGGTATT TTGATTACGT TTTCACCGGT GTCTTCACCT TCGAGATGGT TATCAAGATG
    ATCGACCAGG GCTTGATCCT GCAGGATGGC TCCTACTTCC GAGACCTCTG GAACATCCTG
    GATTTCATCG TGGTGGTGGG AGCTCTGGTG GCCTTTGCCT TGGCGACCAA CAAGGGTCGG
    GATATCAAGA CTATCAAGTC TCTCCGTGTG CTGCGGGTCT TGAGGCCCCT GAAAACCATC
    AAGCGGCTGC CGAAGCTCAA GGCTGTCTTT GACTGTGTTG TGACATCCCT CAAGAACGTC
    TTCAACATCC TCATTGTCTA CAAGCTCTTC ATGTTCATCT TTGCTGTCAT CGCCGTCCAG
    CTCTTCAAGG GGAAGTTTTT CTACTGCACT GACAGCTCTA AGGACACAGA GAAGGACTGC
    ATAGGGAACT ATGTGGACCA TGAGAAGAAC AAGATGGAGG TGAAGTGCCG GGAGTGGAAA
    CGACATGAGT TTCACTATGA CAATATAATC TGGGCGTTGC TCACCCTGTT CACAGTCTCC
    ACCGGCGAGG GTTGGCCCCA GGTGCTGCAG CATTCGGTGG ATGTGACGGA GGAGGACCGT
    GGGCCCAGCC GCAGTAACCG CATGGAGATG TCCATTTTTT ACGTTGTCTA TTTTGTGGTC
    TTCCCTTTCT TCTTCGTCAA CATTTTTGTG GCTCTCATCA TCATCACATT CCAAGAGCAA
    GGAGACAAAA TGATGGAGGA GTGCAGTCTG GAGAAGAACG AGAGGGCCTG TATTGACTTT
    GCCATCAGTG CCAAGCCCCT CACACGCTAC ATGCCCCAGA ACCGGCACAC CTTCCAGTAT
    CGGGTCTGGC ACTTTGTGGT CTCTCCATCC TTTGAGTATA CCATCATGGC CATGATTGCT
    TTGAACACTG TGGTGCTGAT GATGAAGTAC TACTCTGCTC CATACACCTA TGAGCTGGCC
    CTGAAGTACC TGAACATCGC TTTCACCATG GTGTTCTCTT TGGAGTGCGT CCTTAAGATC
    ATTGCTTTTG GCTTCCTGAA TTATTTCCGG GATACCTGGA ATATCTTTGA CTTCATCACC
    GTCATCGGCA GCATCACAGA GATCATCCTG ACAGACACCA AGCTGGTGAA CACCAGTAGC
    TTCAACATGA GTTTCCTGAA ACTCTTCCGG GCGGCTCGGC TCATCAAGCT GCTGCGCCAG
    GGTTACACCA TCCGCATCCT GCTGTGGACG TTCGTGCAGT CCTTCAAGGC TCTCCCCTAT
    GTCTGCCTCC TAATCGCGAT GCTTTTCTTC ATCTATGCCA TCATTGGGAT GCAGGTTTTC
    GGCAACATCA AACTGGATGA GGAAAGCCAC ATTAACCGGC ACAACAACTT CCGCAGCTTC
    CTGGGCTCCC TCATGCTCCT CTTCAGGAGT GCCACCGGTG AGGCATGGCA GGAGATCATG
    CTCTCCTGCC TGGAGGGCAA GGGCTGCGAG CCGGACACGA CAGCGACGTC CGGGCAGAAT
    GAGAACGAGC GCTGCGGCAC CGACCTGGCC TACGTCTACT TTGTCTCCTT CATCTTCTTC
    TGCTCATTCC TGATGCTCAA CCTGTTCGTG GCGGTCATCA TGGACAATTT TGAGTACCTG
    ACTCGAGATT CCTCCATCCT GGGACCTCAC CATCTAGATG AATTTGTCCG GATCTGGGCA
    GAGTATGACA GAGCAGCGTG TGGCCGAATC CATTACACTG AGATGTATGA AATGCTGACT
    CTTATGTCAC CACCGCTAGG CCTCGGCAAG AGATGTCCTT CCAAAGTGGC CTATAAGAGA
    CTGGTGCTGA TGAACATGCC CGTGGCCGAG GACATGACGG TCCATTTCAC CTCCACCCTG
    ATGGCGCTGA TACGCACCGC CCTGGACATC AAAATTGCCA AAGGTGGTGC AGATTGGCAG
    CAGTTAGACT CTGAGCTTCA AAAGGAGATC CTGACCATTT GGCCTCACCT GTCCCAGAAG
    ATGCTTGACC TGTTGGTACC CATGCCAAAA ACCTCTGACC TGACTGTTGG CAAAATATAT
    GCAGCCATGA TGATCATGGA TTACTACAAG CAGAGCAAGG CGAAGAAGCA GAGGCAGCAG
    CTGGAGGAGC AGAAAAATGC GCCCATGTTC CAGCGCATGG AGCCATCCTC TTTGCCACAG
    GAAATCATCT CCAACGCAAA GGCTCTGCCT TACCTCCAGC AGGAGACCCT CACAGGGCTG
    AGCAGCCGGA GCGGGTTCCC CTCGCTGAGC CCGCTCTCAC CACAGGAGAT CTTCCAGCTG
    GCGTGCATGG ATCCGGCCCA CGGGCAGTTC CAGGAGCACC AGTCTCTGGA GCCAGAGGTT
    AGGGAGTTTC AAAGAGTGCA GCCCTCTAAC CGTGGCAGCT ACCTTCCCGT GGACACTCAG
    GACCACGCTG TCTCTGGGAG GGCCTCCTCC ATGCCTCGTC TCACTGTGGA TCCCCAGGTG
    GTGACGGACA CCAGCTCGAT GCGGCGGTCA TTTTCCACCA TCCGGGACAA ACGCACCAAC
    ACCTCCTGGC TGGATGAGTT CTCCATGGAG CGCAGCAGCG ACAACACCTA CAAGTCCCGC
    CGGCGCAGCT ACCACTCCTC TCTCCAGCTC TCTGCCCGGC GCCTCAACGC TGACTCAGGC
    CACAGGTCTG AGGGGCACCG CTCGGGCGGC AGGGAGCGGG GCCGATCCAA AGAGCGCAAG
    CACTTGCTGT CCCCTGACAT CTCCCGCTGC AACTCAGAGG AGAGGAGTCC CCAGGCACAC
    GATGAGTCCC CAGAGCGGCG GCGCGAGTCC CGGTCACCCA GCGAGGGCAG GTCGCAAACG
    CCCAACCGGC AGGGAACGGG CTCCCTGAGC GAAAGCTCCA TCCCCTCCAT CTCGGACACC
    AGCACACCCC GGCGAGGCCG CCGCCAGCTC CCACCTGTGC CCCCAAAACC GCGTCCCCTC
    CTCTCCTACG CCTCCATGCT GCGGCACGCT GCCGGAGACG CCTCACCACC TCCCGATGAG
    ACCGAAGGGG GGTCCCCGCT CCTCTCCAAG GAGCCCAACG CCACCGGCCT GACCGAGTCT
    TCCAGCTCCC CGGCGGGGAA GCCGAGCCGG CCGTCCACCC CGCAGCGTTA CATCTCGGAG
    CCCTACCTGG CCCTGCACGA CGACTCGCAC GCTTCGGACT GCGGCGAGGA GGAGACGCTC
    ACCTTCGAGG CGGCCGTGGC CACCAGTCTG GGTCGTTCCA ACACCATCGG CTCGGCCCCA
    CCGCTGCGAC ACAGCTGGCA GATGCCCAAC GGGCACTACC GGAGACGGAG GCGAGGGGCC
    GGGCAGGGCG TGATGTGCGG GGCCAGCATC GATGTGCTCA GCGACACCGA GGAAGATGAC
    AAGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39011
    Clone ID Related Accession (Same CDS sequence) XM_015290476.1 , XM_015290477.1
    Accession Version XM_015290476.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATCTTTGTGG CGTTCATCAT CATCACCTTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT
    GAGTACCTGA CTCGAGACTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGT GGCCGAATCC ATTACACTGA GATGTATGAA
    ATGCTGACTC TTATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCTTC CAAAGTGGCC
    TATAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGAG
    CCAGAGGTTA GGGAGTTTCA AAGAGTGCAG CCCTCTAACC GTGGCAGCTA CCTTCCCGTG
    GACACTCAGG ACCACGCTGT CTCTGGGAGG GCCTCCTCCA TGCCTCGTCT CACTGTGGAT
    CCCCAGGTGG TGACGGACAC CAGCTCGATG CGGCGGTCAT TTTCCACCAT CCGGGACAAA
    CGCACCAACA CCTCCTGGCT GGATGAGTTC TCCATGGAGC GCAGCAGCGA CAACACCTAC
    AAGTCCCGCC GGCGCAGCTA CCACTCCTCT CTCCAGCTCT CTGCCCGGCG CCTCAACGCT
    GACTCAGGCC ACAGGTCTGA GGGGCACCGC TCGGGCGGCA GGGAGCGGGG CCGATCCAAA
    GAGCGCAAGC ACTTGCTGTC CCCTGACATC TCCCGCTGCA ACTCAGAGGA GAGGAGTCCC
    CAGGCACACG ATGAGTCCCC AGAGCGGCGG CGCGAGTCCC GGTCACCCAG CGAGGGCAGG
    TCGCAAACGC CCAACCGGCA GGGAACGGGC TCCCTGAGCG AAAGCTCCAT CCCCTCCATC
    TCGGACACCA GCACACCCCG GCGAGGCCGC CGCCAGCTCC CACCTGTGCC CCCAAAACCG
    CGTCCCCTCC TCTCCTACGC CTCCATGCTG CGGCACGCTG CCGGAGACGC CTCACCACCT
    CCCGATGAGA CCGAAGGGGG GTCCCCGCTC CTCTCCAAGG AGCCCAACGC CACCGGCCTG
    ACCGAGTCTT CCAGCTCCCC GGCGGGGAAG CCGAGCCGGC CGTCCACCCC GCAGCGTTAC
    ATCTCGGAGC CCTACCTGGC CCTGCACGAC GACTCGCACG CTTCGGACTG CGGCGAGGAG
    GAGACGCTCA CCTTCGAGGC GGCCGTGGCC ACCAGTCTGG GTCGTTCCAA CACCATCGGC
    TCGGCCCCAC CGCTGCGACA CAGCTGGCAG ATGCCCAACG GGCACTACCG GAGACGGAGG
    CGAGGGGCCG GGCAGGGCGT GATGTGCGGG GCCAGCATCG ATGTGCTCAG CGACACCGAG
    GAAGATGACA AGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145962.1
    CDS122..7399
    Translation

    Target ORF information:

    RefSeq Version XM_015290476.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290476.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATCTTTGTGG CGTTCATCAT CATCACCTTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT
    GAGTACCTGA CTCGAGACTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGT GGCCGAATCC ATTACACTGA GATGTATGAA
    ATGCTGACTC TTATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCTTC CAAAGTGGCC
    TATAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGAG
    CCAGAGGTTA GGGAGTTTCA AAGAGTGCAG CCCTCTAACC GTGGCAGCTA CCTTCCCGTG
    GACACTCAGG ACCACGCTGT CTCTGGGAGG GCCTCCTCCA TGCCTCGTCT CACTGTGGAT
    CCCCAGGTGG TGACGGACAC CAGCTCGATG CGGCGGTCAT TTTCCACCAT CCGGGACAAA
    CGCACCAACA CCTCCTGGCT GGATGAGTTC TCCATGGAGC GCAGCAGCGA CAACACCTAC
    AAGTCCCGCC GGCGCAGCTA CCACTCCTCT CTCCAGCTCT CTGCCCGGCG CCTCAACGCT
    GACTCAGGCC ACAGGTCTGA GGGGCACCGC TCGGGCGGCA GGGAGCGGGG CCGATCCAAA
    GAGCGCAAGC ACTTGCTGTC CCCTGACATC TCCCGCTGCA ACTCAGAGGA GAGGAGTCCC
    CAGGCACACG ATGAGTCCCC AGAGCGGCGG CGCGAGTCCC GGTCACCCAG CGAGGGCAGG
    TCGCAAACGC CCAACCGGCA GGGAACGGGC TCCCTGAGCG AAAGCTCCAT CCCCTCCATC
    TCGGACACCA GCACACCCCG GCGAGGCCGC CGCCAGCTCC CACCTGTGCC CCCAAAACCG
    CGTCCCCTCC TCTCCTACGC CTCCATGCTG CGGCACGCTG CCGGAGACGC CTCACCACCT
    CCCGATGAGA CCGAAGGGGG GTCCCCGCTC CTCTCCAAGG AGCCCAACGC CACCGGCCTG
    ACCGAGTCTT CCAGCTCCCC GGCGGGGAAG CCGAGCCGGC CGTCCACCCC GCAGCGTTAC
    ATCTCGGAGC CCTACCTGGC CCTGCACGAC GACTCGCACG CTTCGGACTG CGGCGAGGAG
    GAGACGCTCA CCTTCGAGGC GGCCGTGGCC ACCAGTCTGG GTCGTTCCAA CACCATCGGC
    TCGGCCCCAC CGCTGCGACA CAGCTGGCAG ATGCCCAACG GGCACTACCG GAGACGGAGG
    CGAGGGGCCG GGCAGGGCGT GATGTGCGGG GCCAGCATCG ATGTGCTCAG CGACACCGAG
    GAAGATGACA AGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39011
    Clone ID Related Accession (Same CDS sequence) XM_015290476.1 , XM_015290477.1
    Accession Version XM_015290477.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATCTTTGTGG CGTTCATCAT CATCACCTTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT
    GAGTACCTGA CTCGAGACTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGT GGCCGAATCC ATTACACTGA GATGTATGAA
    ATGCTGACTC TTATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCTTC CAAAGTGGCC
    TATAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGAG
    CCAGAGGTTA GGGAGTTTCA AAGAGTGCAG CCCTCTAACC GTGGCAGCTA CCTTCCCGTG
    GACACTCAGG ACCACGCTGT CTCTGGGAGG GCCTCCTCCA TGCCTCGTCT CACTGTGGAT
    CCCCAGGTGG TGACGGACAC CAGCTCGATG CGGCGGTCAT TTTCCACCAT CCGGGACAAA
    CGCACCAACA CCTCCTGGCT GGATGAGTTC TCCATGGAGC GCAGCAGCGA CAACACCTAC
    AAGTCCCGCC GGCGCAGCTA CCACTCCTCT CTCCAGCTCT CTGCCCGGCG CCTCAACGCT
    GACTCAGGCC ACAGGTCTGA GGGGCACCGC TCGGGCGGCA GGGAGCGGGG CCGATCCAAA
    GAGCGCAAGC ACTTGCTGTC CCCTGACATC TCCCGCTGCA ACTCAGAGGA GAGGAGTCCC
    CAGGCACACG ATGAGTCCCC AGAGCGGCGG CGCGAGTCCC GGTCACCCAG CGAGGGCAGG
    TCGCAAACGC CCAACCGGCA GGGAACGGGC TCCCTGAGCG AAAGCTCCAT CCCCTCCATC
    TCGGACACCA GCACACCCCG GCGAGGCCGC CGCCAGCTCC CACCTGTGCC CCCAAAACCG
    CGTCCCCTCC TCTCCTACGC CTCCATGCTG CGGCACGCTG CCGGAGACGC CTCACCACCT
    CCCGATGAGA CCGAAGGGGG GTCCCCGCTC CTCTCCAAGG AGCCCAACGC CACCGGCCTG
    ACCGAGTCTT CCAGCTCCCC GGCGGGGAAG CCGAGCCGGC CGTCCACCCC GCAGCGTTAC
    ATCTCGGAGC CCTACCTGGC CCTGCACGAC GACTCGCACG CTTCGGACTG CGGCGAGGAG
    GAGACGCTCA CCTTCGAGGC GGCCGTGGCC ACCAGTCTGG GTCGTTCCAA CACCATCGGC
    TCGGCCCCAC CGCTGCGACA CAGCTGGCAG ATGCCCAACG GGCACTACCG GAGACGGAGG
    CGAGGGGCCG GGCAGGGCGT GATGTGCGGG GCCAGCATCG ATGTGCTCAG CGACACCGAG
    GAAGATGACA AGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145963.1
    CDS44..7321
    Translation

    Target ORF information:

    RefSeq Version XM_015290477.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290477.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATCTTTGTGG CGTTCATCAT CATCACCTTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT
    GAGTACCTGA CTCGAGACTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGT GGCCGAATCC ATTACACTGA GATGTATGAA
    ATGCTGACTC TTATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCTTC CAAAGTGGCC
    TATAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGAG
    CCAGAGGTTA GGGAGTTTCA AAGAGTGCAG CCCTCTAACC GTGGCAGCTA CCTTCCCGTG
    GACACTCAGG ACCACGCTGT CTCTGGGAGG GCCTCCTCCA TGCCTCGTCT CACTGTGGAT
    CCCCAGGTGG TGACGGACAC CAGCTCGATG CGGCGGTCAT TTTCCACCAT CCGGGACAAA
    CGCACCAACA CCTCCTGGCT GGATGAGTTC TCCATGGAGC GCAGCAGCGA CAACACCTAC
    AAGTCCCGCC GGCGCAGCTA CCACTCCTCT CTCCAGCTCT CTGCCCGGCG CCTCAACGCT
    GACTCAGGCC ACAGGTCTGA GGGGCACCGC TCGGGCGGCA GGGAGCGGGG CCGATCCAAA
    GAGCGCAAGC ACTTGCTGTC CCCTGACATC TCCCGCTGCA ACTCAGAGGA GAGGAGTCCC
    CAGGCACACG ATGAGTCCCC AGAGCGGCGG CGCGAGTCCC GGTCACCCAG CGAGGGCAGG
    TCGCAAACGC CCAACCGGCA GGGAACGGGC TCCCTGAGCG AAAGCTCCAT CCCCTCCATC
    TCGGACACCA GCACACCCCG GCGAGGCCGC CGCCAGCTCC CACCTGTGCC CCCAAAACCG
    CGTCCCCTCC TCTCCTACGC CTCCATGCTG CGGCACGCTG CCGGAGACGC CTCACCACCT
    CCCGATGAGA CCGAAGGGGG GTCCCCGCTC CTCTCCAAGG AGCCCAACGC CACCGGCCTG
    ACCGAGTCTT CCAGCTCCCC GGCGGGGAAG CCGAGCCGGC CGTCCACCCC GCAGCGTTAC
    ATCTCGGAGC CCTACCTGGC CCTGCACGAC GACTCGCACG CTTCGGACTG CGGCGAGGAG
    GAGACGCTCA CCTTCGAGGC GGCCGTGGCC ACCAGTCTGG GTCGTTCCAA CACCATCGGC
    TCGGCCCCAC CGCTGCGACA CAGCTGGCAG ATGCCCAACG GGCACTACCG GAGACGGAGG
    CGAGGGGCCG GGCAGGGCGT GATGTGCGGG GCCAGCATCG ATGTGCTCAG CGACACCGAG
    GAAGATGACA AGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39012
    Clone ID Related Accession (Same CDS sequence) XM_015290478.1
    Accession Version XM_015290478.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATCTTTGTGG CGTTCATCAT CATCACCTTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT
    GAGTACCTGA CTCGAGACTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGC GGCAGGATCT CGTATAAGGA TATGTACAAA
    TTAGTACGGG TGATCTCGCC TCCTCTGGGC CTAGGAAAGA ACTGCCCCTA CAGGGTGGCG
    TGCAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGAG
    CCAGAGGTTA GGGAGTTTCA AAGAGTGCAG CCCTCTAACC GTGGCAGCTA CCTTCCCGTG
    GACACTCAGG ACCACGCTGT CTCTGGGAGG GCCTCCTCCA TGCCTCGTCT CACTGTGGAT
    CCCCAGGTGG TGACGGACAC CAGCTCGATG CGGCGGTCAT TTTCCACCAT CCGGGACAAA
    CGCACCAACA CCTCCTGGCT GGATGAGTTC TCCATGGAGC GCAGCAGCGA CAACACCTAC
    AAGTCCCGCC GGCGCAGCTA CCACTCCTCT CTCCAGCTCT CTGCCCGGCG CCTCAACGCT
    GACTCAGGCC ACAGGTCTGA GGGGCACCGC TCGGGCGGCA GGGAGCGGGG CCGATCCAAA
    GAGCGCAAGC ACTTGCTGTC CCCTGACATC TCCCGCTGCA ACTCAGAGGA GAGGAGTCCC
    CAGGCACACG ATGAGTCCCC AGAGCGGCGG CGCGAGTCCC GGTCACCCAG CGAGGGCAGG
    TCGCAAACGC CCAACCGGCA GGGAACGGGC TCCCTGAGCG AAAGCTCCAT CCCCTCCATC
    TCGGACACCA GCACACCCCG GCGAGGCCGC CGCCAGCTCC CACCTGTGCC CCCAAAACCG
    CGTCCCCTCC TCTCCTACGC CTCCATGCTG CGGCACGCTG CCGGAGACGC CTCACCACCT
    CCCGATGAGA CCGAAGGGGG GTCCCCGCTC CTCTCCAAGG AGCCCAACGC CACCGGCCTG
    ACCGAGTCTT CCAGCTCCCC GGCGGGGAAG CCGAGCCGGC CGTCCACCCC GCAGCGTTAC
    ATCTCGGAGC CCTACCTGGC CCTGCACGAC GACTCGCACG CTTCGGACTG CGGCGAGGAG
    GAGACGCTCA CCTTCGAGGC GGCCGTGGCC ACCAGTCTGG GTCGTTCCAA CACCATCGGC
    TCGGCCCCAC CGCTGCGACA CAGCTGGCAG ATGCCCAACG GGCACTACCG GAGACGGAGG
    CGAGGGGCCG GGCAGGGCGT GATGTGCGGG GCCAGCATCG ATGTGCTCAG CGACACCGAG
    GAAGATGACA AGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145964.1
    CDS122..7399
    Translation

    Target ORF information:

    RefSeq Version XM_015290478.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290478.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATCTTTGTGG CGTTCATCAT CATCACCTTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT
    GAGTACCTGA CTCGAGACTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGC GGCAGGATCT CGTATAAGGA TATGTACAAA
    TTAGTACGGG TGATCTCGCC TCCTCTGGGC CTAGGAAAGA ACTGCCCCTA CAGGGTGGCG
    TGCAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGAG
    CCAGAGGTTA GGGAGTTTCA AAGAGTGCAG CCCTCTAACC GTGGCAGCTA CCTTCCCGTG
    GACACTCAGG ACCACGCTGT CTCTGGGAGG GCCTCCTCCA TGCCTCGTCT CACTGTGGAT
    CCCCAGGTGG TGACGGACAC CAGCTCGATG CGGCGGTCAT TTTCCACCAT CCGGGACAAA
    CGCACCAACA CCTCCTGGCT GGATGAGTTC TCCATGGAGC GCAGCAGCGA CAACACCTAC
    AAGTCCCGCC GGCGCAGCTA CCACTCCTCT CTCCAGCTCT CTGCCCGGCG CCTCAACGCT
    GACTCAGGCC ACAGGTCTGA GGGGCACCGC TCGGGCGGCA GGGAGCGGGG CCGATCCAAA
    GAGCGCAAGC ACTTGCTGTC CCCTGACATC TCCCGCTGCA ACTCAGAGGA GAGGAGTCCC
    CAGGCACACG ATGAGTCCCC AGAGCGGCGG CGCGAGTCCC GGTCACCCAG CGAGGGCAGG
    TCGCAAACGC CCAACCGGCA GGGAACGGGC TCCCTGAGCG AAAGCTCCAT CCCCTCCATC
    TCGGACACCA GCACACCCCG GCGAGGCCGC CGCCAGCTCC CACCTGTGCC CCCAAAACCG
    CGTCCCCTCC TCTCCTACGC CTCCATGCTG CGGCACGCTG CCGGAGACGC CTCACCACCT
    CCCGATGAGA CCGAAGGGGG GTCCCCGCTC CTCTCCAAGG AGCCCAACGC CACCGGCCTG
    ACCGAGTCTT CCAGCTCCCC GGCGGGGAAG CCGAGCCGGC CGTCCACCCC GCAGCGTTAC
    ATCTCGGAGC CCTACCTGGC CCTGCACGAC GACTCGCACG CTTCGGACTG CGGCGAGGAG
    GAGACGCTCA CCTTCGAGGC GGCCGTGGCC ACCAGTCTGG GTCGTTCCAA CACCATCGGC
    TCGGCCCCAC CGCTGCGACA CAGCTGGCAG ATGCCCAACG GGCACTACCG GAGACGGAGG
    CGAGGGGCCG GGCAGGGCGT GATGTGCGGG GCCAGCATCG ATGTGCTCAG CGACACCGAG
    GAAGATGACA AGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39013
    Clone ID Related Accession (Same CDS sequence) XM_015290479.1
    Accession Version XM_015290479.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGACCAACAA GGGTCGGGAT
    ATCAAGACTA TCAAGTCTCT CCGTGTGCTG CGGGTCTTGA GGCCCCTGAA AACCATCAAG
    CGGCTGCCGA AGCTCAAGGC TGTCTTTGAC TGTGTTGTGA CATCCCTCAA GAACGTCTTC
    AACATCCTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CTGTCATCGC CGTCCAGCTC
    TTCAAGGGGA AGTTTTTCTA CTGCACTGAC AGCTCTAAGG ACACAGAGAA GGACTGCATA
    GGGAACTATG TGGACCATGA GAAGAACAAG ATGGAGGTGA AGTGCCGGGA GTGGAAACGA
    CATGAGTTTC ACTATGACAA TATAATCTGG GCGTTGCTCA CCCTGTTCAC AGTCTCCACC
    GGCGAGGGTT GGCCCCAGGT GCTGCAGCAT TCGGTGGATG TGACGGAGGA GGACCGTGGG
    CCCAGCCGCA GTAACCGCAT GGAGATGTCC ATTTTTTACG TTGTCTATTT TGTGGTCTTC
    CCTTTCTTCT TCGTCAACAT CTTTGTGGCG TTCATCATCA TCACCTTCCA AGAGCAAGGA
    GACAAAATGA TGGAGGAGTG CAGTCTGGAG AAGAACGAGA GGGCCTGTAT TGACTTTGCC
    ATCAGTGCCA AGCCCCTCAC ACGCTACATG CCCCAGAACC GGCACACCTT CCAGTATCGG
    GTCTGGCACT TTGTGGTCTC TCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCTTTG
    AACACTGTGG TGCTGATGAT GAAGTACTAC TCTGCTCCAT ACACCTATGA GCTGGCCCTG
    AAGTACCTGA ACATCGCTTT CACCATGGTG TTCTCTTTGG AGTGCGTCCT TAAGATCATT
    GCTTTTGGCT TCCTGAATTA TTTCCGGGAT ACCTGGAATA TCTTTGACTT CATCACCGTC
    ATCGGCAGCA TCACAGAGAT CATCCTGACA GACACCAAGC TGGTGAACAC CAGTAGCTTC
    AACATGAGTT TCCTGAAACT CTTCCGGGCG GCTCGGCTCA TCAAGCTGCT GCGCCAGGGT
    TACACCATCC GCATCCTGCT GTGGACGTTC GTGCAGTCCT TCAAGGCTCT CCCCTATGTC
    TGCCTCCTAA TCGCGATGCT TTTCTTCATC TATGCCATCA TTGGGATGCA GGTTTTCGGC
    AACATCAAAC TGGATGAGGA AAGCCACATT AACCGGCACA ACAACTTCCG CAGCTTCCTG
    GGCTCCCTCA TGCTCCTCTT CAGGAGTGCC ACCGGTGAGG CATGGCAGGA GATCATGCTC
    TCCTGCCTGG AGGGCAAGGG CTGCGAGCCG GACACGACAG CGACGTCCGG GCAGAATGAG
    AACGAGCGCT GCGGCACCGA CCTGGCCTAC GTCTACTTTG TCTCCTTCAT CTTCTTCTGC
    TCATTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT
    CGAGACTCCT CCATCCTGGG ACCTCACCAT CTAGATGAAT TTGTCCGGAT CTGGGCAGAG
    TATGACAGAG CAGCGTGTGG CCGAATCCAT TACACTGAGA TGTATGAAAT GCTGACTCTT
    ATGTCACCAC CGCTAGGCCT CGGCAAGAGA TGTCCTTCCA AAGTGGCCTA TAAGAGACTG
    GTGCTGATGA ACATGCCCGT GGCCGAGGAC ATGACGGTCC ATTTCACCTC CACCCTGATG
    GCGCTGATAC GCACCGCCCT GGACATCAAA ATTGCCAAAG GTGGTGCAGA TTGGCAGCAG
    TTAGACTCTG AGCTTCAAAA GGAGATCCTG ACCATTTGGC CTCACCTGTC CCAGAAGATG
    CTTGACCTGT TGGTACCCAT GCCAAAAACC TCTGACCTGA CTGTTGGCAA AATATATGCA
    GCCATGATGA TCATGGATTA CTACAAGCAG AGCAAGGCGA AGAAGCAGAG GCAGCAGCTG
    GAGGAGCAGA AAAATGCGCC CATGTTCCAG CGCATGGAGC CATCCTCTTT GCCACAGGAA
    ATCATCTCCA ACGCAAAGGC TCTGCCTTAC CTCCAGCAGG AGACCCTCAC AGGGCTGAGC
    AGCCGGAGCG GGTTCCCCTC GCTGAGCCCG CTCTCACCAC AGGAGATCTT CCAGCTGGCG
    TGCATGGATC CGGCCCACGG GCAGTTCCAG GAGCACCAGT CTCTGGAGCC AGAGGTTAGG
    GAGTTTCAAA GAGTGCAGCC CTCTAACCGT GGCAGCTACC TTCCCGTGGA CACTCAGGAC
    CACGCTGTCT CTGGGAGGGC CTCCTCCATG CCTCGTCTCA CTGTGGATCC CCAGGTGGTG
    ACGGACACCA GCTCGATGCG GCGGTCATTT TCCACCATCC GGGACAAACG CACCAACACC
    TCCTGGCTGG ATGAGTTCTC CATGGAGCGC AGCAGCGACA ACACCTACAA GTCCCGCCGG
    CGCAGCTACC ACTCCTCTCT CCAGCTCTCT GCCCGGCGCC TCAACGCTGA CTCAGGCCAC
    AGGTCTGAGG GGCACCGCTC GGGCGGCAGG GAGCGGGGCC GATCCAAAGA GCGCAAGCAC
    TTGCTGTCCC CTGACATCTC CCGCTGCAAC TCAGAGGAGA GGAGTCCCCA GGCACACGAT
    GAGTCCCCAG AGCGGCGGCG CGAGTCCCGG TCACCCAGCG AGGGCAGGTC GCAAACGCCC
    AACCGGCAGG GAACGGGCTC CCTGAGCGAA AGCTCCATCC CCTCCATCTC GGACACCAGC
    ACACCCCGGC GAGGCCGCCG CCAGCTCCCA CCTGTGCCCC CAAAACCGCG TCCCCTCCTC
    TCCTACGCCT CCATGCTGCG GCACGCTGCC GGAGACGCCT CACCACCTCC CGATGAGACC
    GAAGGGGGGT CCCCGCTCCT CTCCAAGGAG CCCAACGCCA CCGGCCTGAC CGAGTCTTCC
    AGCTCCCCGG CGGGGAAGCC GAGCCGGCCG TCCACCCCGC AGCGTTACAT CTCGGAGCCC
    TACCTGGCCC TGCACGACGA CTCGCACGCT TCGGACTGCG GCGAGGAGGA GACGCTCACC
    TTCGAGGCGG CCGTGGCCAC CAGTCTGGGT CGTTCCAACA CCATCGGCTC GGCCCCACCG
    CTGCGACACA GCTGGCAGAT GCCCAACGGG CACTACCGGA GACGGAGGCG AGGGGCCGGG
    CAGGGCGTGA TGTGCGGGGC CAGCATCGAT GTGCTCAGCG ACACCGAGGA AGATGACAAG
    TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145965.1
    CDS122..7387
    Translation

    Target ORF information:

    RefSeq Version XM_015290479.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290479.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGACCAACAA GGGTCGGGAT
    ATCAAGACTA TCAAGTCTCT CCGTGTGCTG CGGGTCTTGA GGCCCCTGAA AACCATCAAG
    CGGCTGCCGA AGCTCAAGGC TGTCTTTGAC TGTGTTGTGA CATCCCTCAA GAACGTCTTC
    AACATCCTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CTGTCATCGC CGTCCAGCTC
    TTCAAGGGGA AGTTTTTCTA CTGCACTGAC AGCTCTAAGG ACACAGAGAA GGACTGCATA
    GGGAACTATG TGGACCATGA GAAGAACAAG ATGGAGGTGA AGTGCCGGGA GTGGAAACGA
    CATGAGTTTC ACTATGACAA TATAATCTGG GCGTTGCTCA CCCTGTTCAC AGTCTCCACC
    GGCGAGGGTT GGCCCCAGGT GCTGCAGCAT TCGGTGGATG TGACGGAGGA GGACCGTGGG
    CCCAGCCGCA GTAACCGCAT GGAGATGTCC ATTTTTTACG TTGTCTATTT TGTGGTCTTC
    CCTTTCTTCT TCGTCAACAT CTTTGTGGCG TTCATCATCA TCACCTTCCA AGAGCAAGGA
    GACAAAATGA TGGAGGAGTG CAGTCTGGAG AAGAACGAGA GGGCCTGTAT TGACTTTGCC
    ATCAGTGCCA AGCCCCTCAC ACGCTACATG CCCCAGAACC GGCACACCTT CCAGTATCGG
    GTCTGGCACT TTGTGGTCTC TCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCTTTG
    AACACTGTGG TGCTGATGAT GAAGTACTAC TCTGCTCCAT ACACCTATGA GCTGGCCCTG
    AAGTACCTGA ACATCGCTTT CACCATGGTG TTCTCTTTGG AGTGCGTCCT TAAGATCATT
    GCTTTTGGCT TCCTGAATTA TTTCCGGGAT ACCTGGAATA TCTTTGACTT CATCACCGTC
    ATCGGCAGCA TCACAGAGAT CATCCTGACA GACACCAAGC TGGTGAACAC CAGTAGCTTC
    AACATGAGTT TCCTGAAACT CTTCCGGGCG GCTCGGCTCA TCAAGCTGCT GCGCCAGGGT
    TACACCATCC GCATCCTGCT GTGGACGTTC GTGCAGTCCT TCAAGGCTCT CCCCTATGTC
    TGCCTCCTAA TCGCGATGCT TTTCTTCATC TATGCCATCA TTGGGATGCA GGTTTTCGGC
    AACATCAAAC TGGATGAGGA AAGCCACATT AACCGGCACA ACAACTTCCG CAGCTTCCTG
    GGCTCCCTCA TGCTCCTCTT CAGGAGTGCC ACCGGTGAGG CATGGCAGGA GATCATGCTC
    TCCTGCCTGG AGGGCAAGGG CTGCGAGCCG GACACGACAG CGACGTCCGG GCAGAATGAG
    AACGAGCGCT GCGGCACCGA CCTGGCCTAC GTCTACTTTG TCTCCTTCAT CTTCTTCTGC
    TCATTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT
    CGAGACTCCT CCATCCTGGG ACCTCACCAT CTAGATGAAT TTGTCCGGAT CTGGGCAGAG
    TATGACAGAG CAGCGTGTGG CCGAATCCAT TACACTGAGA TGTATGAAAT GCTGACTCTT
    ATGTCACCAC CGCTAGGCCT CGGCAAGAGA TGTCCTTCCA AAGTGGCCTA TAAGAGACTG
    GTGCTGATGA ACATGCCCGT GGCCGAGGAC ATGACGGTCC ATTTCACCTC CACCCTGATG
    GCGCTGATAC GCACCGCCCT GGACATCAAA ATTGCCAAAG GTGGTGCAGA TTGGCAGCAG
    TTAGACTCTG AGCTTCAAAA GGAGATCCTG ACCATTTGGC CTCACCTGTC CCAGAAGATG
    CTTGACCTGT TGGTACCCAT GCCAAAAACC TCTGACCTGA CTGTTGGCAA AATATATGCA
    GCCATGATGA TCATGGATTA CTACAAGCAG AGCAAGGCGA AGAAGCAGAG GCAGCAGCTG
    GAGGAGCAGA AAAATGCGCC CATGTTCCAG CGCATGGAGC CATCCTCTTT GCCACAGGAA
    ATCATCTCCA ACGCAAAGGC TCTGCCTTAC CTCCAGCAGG AGACCCTCAC AGGGCTGAGC
    AGCCGGAGCG GGTTCCCCTC GCTGAGCCCG CTCTCACCAC AGGAGATCTT CCAGCTGGCG
    TGCATGGATC CGGCCCACGG GCAGTTCCAG GAGCACCAGT CTCTGGAGCC AGAGGTTAGG
    GAGTTTCAAA GAGTGCAGCC CTCTAACCGT GGCAGCTACC TTCCCGTGGA CACTCAGGAC
    CACGCTGTCT CTGGGAGGGC CTCCTCCATG CCTCGTCTCA CTGTGGATCC CCAGGTGGTG
    ACGGACACCA GCTCGATGCG GCGGTCATTT TCCACCATCC GGGACAAACG CACCAACACC
    TCCTGGCTGG ATGAGTTCTC CATGGAGCGC AGCAGCGACA ACACCTACAA GTCCCGCCGG
    CGCAGCTACC ACTCCTCTCT CCAGCTCTCT GCCCGGCGCC TCAACGCTGA CTCAGGCCAC
    AGGTCTGAGG GGCACCGCTC GGGCGGCAGG GAGCGGGGCC GATCCAAAGA GCGCAAGCAC
    TTGCTGTCCC CTGACATCTC CCGCTGCAAC TCAGAGGAGA GGAGTCCCCA GGCACACGAT
    GAGTCCCCAG AGCGGCGGCG CGAGTCCCGG TCACCCAGCG AGGGCAGGTC GCAAACGCCC
    AACCGGCAGG GAACGGGCTC CCTGAGCGAA AGCTCCATCC CCTCCATCTC GGACACCAGC
    ACACCCCGGC GAGGCCGCCG CCAGCTCCCA CCTGTGCCCC CAAAACCGCG TCCCCTCCTC
    TCCTACGCCT CCATGCTGCG GCACGCTGCC GGAGACGCCT CACCACCTCC CGATGAGACC
    GAAGGGGGGT CCCCGCTCCT CTCCAAGGAG CCCAACGCCA CCGGCCTGAC CGAGTCTTCC
    AGCTCCCCGG CGGGGAAGCC GAGCCGGCCG TCCACCCCGC AGCGTTACAT CTCGGAGCCC
    TACCTGGCCC TGCACGACGA CTCGCACGCT TCGGACTGCG GCGAGGAGGA GACGCTCACC
    TTCGAGGCGG CCGTGGCCAC CAGTCTGGGT CGTTCCAACA CCATCGGCTC GGCCCCACCG
    CTGCGACACA GCTGGCAGAT GCCCAACGGG CACTACCGGA GACGGAGGCG AGGGGCCGGG
    CAGGGCGTGA TGTGCGGGGC CAGCATCGAT GTGCTCAGCG ACACCGAGGA AGATGACAAG
    TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39014
    Clone ID Related Accession (Same CDS sequence) XM_015290480.1
    Accession Version XM_015290480.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGACCAACAA GGGTCGGGAT
    ATCAAGACTA TCAAGTCTCT CCGTGTGCTG CGGGTCTTGA GGCCCCTGAA AACCATCAAG
    CGGCTGCCGA AGCTCAAGGC TGTCTTTGAC TGTGTTGTGA CATCCCTCAA GAACGTCTTC
    AACATCCTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CTGTCATCGC CGTCCAGCTC
    TTCAAGGGGA AGTTTTTCTA CTGCACTGAC AGCTCTAAGG ACACAGAGAA GGACTGCATA
    GGGAACTATG TGGACCATGA GAAGAACAAG ATGGAGGTGA AGTGCCGGGA GTGGAAACGA
    CATGAGTTTC ACTATGACAA TATAATCTGG GCGTTGCTCA CCCTGTTCAC AGTCTCCACC
    GGCGAGGGTT GGCCCCAGGT GCTGCAGCAT TCGGTGGATG TGACGGAGGA GGACCGTGGG
    CCCAGCCGCA GTAACCGCAT GGAGATGTCC ATTTTTTACG TTGTCTATTT TGTGGTCTTC
    CCTTTCTTCT TCGTCAACAT CTTTGTGGCG TTCATCATCA TCACCTTCCA AGAGCAAGGA
    GACAAAATGA TGGAGGAGTG CAGTCTGGAG AAGAACGAGA GGGCCTGTAT TGACTTTGCC
    ATCAGTGCCA AGCCCCTCAC ACGCTACATG CCCCAGAACC GGCACACCTT CCAGTATCGG
    GTCTGGCACT TTGTGGTCTC TCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCTTTG
    AACACTGTGG TGCTGATGAT GAAGTACTAC TCTGCTCCAT ACACCTATGA GCTGGCCCTG
    AAGTACCTGA ACATCGCTTT CACCATGGTG TTCTCTTTGG AGTGCGTCCT TAAGATCATT
    GCTTTTGGCT TCCTGAATTA TTTCCGGGAT ACCTGGAATA TCTTTGACTT CATCACCGTC
    ATCGGCAGCA TCACAGAGAT CATCCTGACA GACACCAAGC TGGTGAACAC CAGTAGCTTC
    AACATGAGTT TCCTGAAACT CTTCCGGGCG GCTCGGCTCA TCAAGCTGCT GCGCCAGGGT
    TACACCATCC GCATCCTGCT GTGGACGTTC GTGCAGTCCT TCAAGGCTCT CCCCTATGTC
    TGCCTCCTAA TCGCGATGCT TTTCTTCATC TATGCCATCA TTGGGATGCA GGTTTTCGGC
    AACATCAAAC TGGATGAGGA AAGCCACATT AACCGGCACA ACAACTTCCG CAGCTTCCTG
    GGCTCCCTCA TGCTCCTCTT CAGGAGTGCC ACCGGTGAGG CATGGCAGGA GATCATGCTC
    TCCTGCCTGG AGGGCAAGGG CTGCGAGCCG GACACGACAG CGACGTCCGG GCAGAATGAG
    AACGAGCGCT GCGGCACCGA CCTGGCCTAC GTCTACTTTG TCTCCTTCAT CTTCTTCTGC
    TCATTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT
    CGAGACTCCT CCATCCTGGG ACCTCACCAT CTAGATGAAT TTGTCCGGAT CTGGGCAGAG
    TATGACAGAG CAGCGTGCGG CAGGATCTCG TATAAGGATA TGTACAAATT AGTACGGGTG
    ATCTCGCCTC CTCTGGGCCT AGGAAAGAAC TGCCCCTACA GGGTGGCGTG CAAGAGACTG
    GTGCTGATGA ACATGCCCGT GGCCGAGGAC ATGACGGTCC ATTTCACCTC CACCCTGATG
    GCGCTGATAC GCACCGCCCT GGACATCAAA ATTGCCAAAG GTGGTGCAGA TTGGCAGCAG
    TTAGACTCTG AGCTTCAAAA GGAGATCCTG ACCATTTGGC CTCACCTGTC CCAGAAGATG
    CTTGACCTGT TGGTACCCAT GCCAAAAACC TCTGACCTGA CTGTTGGCAA AATATATGCA
    GCCATGATGA TCATGGATTA CTACAAGCAG AGCAAGGCGA AGAAGCAGAG GCAGCAGCTG
    GAGGAGCAGA AAAATGCGCC CATGTTCCAG CGCATGGAGC CATCCTCTTT GCCACAGGAA
    ATCATCTCCA ACGCAAAGGC TCTGCCTTAC CTCCAGCAGG AGACCCTCAC AGGGCTGAGC
    AGCCGGAGCG GGTTCCCCTC GCTGAGCCCG CTCTCACCAC AGGAGATCTT CCAGCTGGCG
    TGCATGGATC CGGCCCACGG GCAGTTCCAG GAGCACCAGT CTCTGGAGCC AGAGGTTAGG
    GAGTTTCAAA GAGTGCAGCC CTCTAACCGT GGCAGCTACC TTCCCGTGGA CACTCAGGAC
    CACGCTGTCT CTGGGAGGGC CTCCTCCATG CCTCGTCTCA CTGTGGATCC CCAGGTGGTG
    ACGGACACCA GCTCGATGCG GCGGTCATTT TCCACCATCC GGGACAAACG CACCAACACC
    TCCTGGCTGG ATGAGTTCTC CATGGAGCGC AGCAGCGACA ACACCTACAA GTCCCGCCGG
    CGCAGCTACC ACTCCTCTCT CCAGCTCTCT GCCCGGCGCC TCAACGCTGA CTCAGGCCAC
    AGGTCTGAGG GGCACCGCTC GGGCGGCAGG GAGCGGGGCC GATCCAAAGA GCGCAAGCAC
    TTGCTGTCCC CTGACATCTC CCGCTGCAAC TCAGAGGAGA GGAGTCCCCA GGCACACGAT
    GAGTCCCCAG AGCGGCGGCG CGAGTCCCGG TCACCCAGCG AGGGCAGGTC GCAAACGCCC
    AACCGGCAGG GAACGGGCTC CCTGAGCGAA AGCTCCATCC CCTCCATCTC GGACACCAGC
    ACACCCCGGC GAGGCCGCCG CCAGCTCCCA CCTGTGCCCC CAAAACCGCG TCCCCTCCTC
    TCCTACGCCT CCATGCTGCG GCACGCTGCC GGAGACGCCT CACCACCTCC CGATGAGACC
    GAAGGGGGGT CCCCGCTCCT CTCCAAGGAG CCCAACGCCA CCGGCCTGAC CGAGTCTTCC
    AGCTCCCCGG CGGGGAAGCC GAGCCGGCCG TCCACCCCGC AGCGTTACAT CTCGGAGCCC
    TACCTGGCCC TGCACGACGA CTCGCACGCT TCGGACTGCG GCGAGGAGGA GACGCTCACC
    TTCGAGGCGG CCGTGGCCAC CAGTCTGGGT CGTTCCAACA CCATCGGCTC GGCCCCACCG
    CTGCGACACA GCTGGCAGAT GCCCAACGGG CACTACCGGA GACGGAGGCG AGGGGCCGGG
    CAGGGCGTGA TGTGCGGGGC CAGCATCGAT GTGCTCAGCG ACACCGAGGA AGATGACAAG
    TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145966.1
    CDS122..7387
    Translation

    Target ORF information:

    RefSeq Version XM_015290480.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290480.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGACCAACAA GGGTCGGGAT
    ATCAAGACTA TCAAGTCTCT CCGTGTGCTG CGGGTCTTGA GGCCCCTGAA AACCATCAAG
    CGGCTGCCGA AGCTCAAGGC TGTCTTTGAC TGTGTTGTGA CATCCCTCAA GAACGTCTTC
    AACATCCTCA TTGTCTACAA GCTCTTCATG TTCATCTTTG CTGTCATCGC CGTCCAGCTC
    TTCAAGGGGA AGTTTTTCTA CTGCACTGAC AGCTCTAAGG ACACAGAGAA GGACTGCATA
    GGGAACTATG TGGACCATGA GAAGAACAAG ATGGAGGTGA AGTGCCGGGA GTGGAAACGA
    CATGAGTTTC ACTATGACAA TATAATCTGG GCGTTGCTCA CCCTGTTCAC AGTCTCCACC
    GGCGAGGGTT GGCCCCAGGT GCTGCAGCAT TCGGTGGATG TGACGGAGGA GGACCGTGGG
    CCCAGCCGCA GTAACCGCAT GGAGATGTCC ATTTTTTACG TTGTCTATTT TGTGGTCTTC
    CCTTTCTTCT TCGTCAACAT CTTTGTGGCG TTCATCATCA TCACCTTCCA AGAGCAAGGA
    GACAAAATGA TGGAGGAGTG CAGTCTGGAG AAGAACGAGA GGGCCTGTAT TGACTTTGCC
    ATCAGTGCCA AGCCCCTCAC ACGCTACATG CCCCAGAACC GGCACACCTT CCAGTATCGG
    GTCTGGCACT TTGTGGTCTC TCCATCCTTT GAGTATACCA TCATGGCCAT GATTGCTTTG
    AACACTGTGG TGCTGATGAT GAAGTACTAC TCTGCTCCAT ACACCTATGA GCTGGCCCTG
    AAGTACCTGA ACATCGCTTT CACCATGGTG TTCTCTTTGG AGTGCGTCCT TAAGATCATT
    GCTTTTGGCT TCCTGAATTA TTTCCGGGAT ACCTGGAATA TCTTTGACTT CATCACCGTC
    ATCGGCAGCA TCACAGAGAT CATCCTGACA GACACCAAGC TGGTGAACAC CAGTAGCTTC
    AACATGAGTT TCCTGAAACT CTTCCGGGCG GCTCGGCTCA TCAAGCTGCT GCGCCAGGGT
    TACACCATCC GCATCCTGCT GTGGACGTTC GTGCAGTCCT TCAAGGCTCT CCCCTATGTC
    TGCCTCCTAA TCGCGATGCT TTTCTTCATC TATGCCATCA TTGGGATGCA GGTTTTCGGC
    AACATCAAAC TGGATGAGGA AAGCCACATT AACCGGCACA ACAACTTCCG CAGCTTCCTG
    GGCTCCCTCA TGCTCCTCTT CAGGAGTGCC ACCGGTGAGG CATGGCAGGA GATCATGCTC
    TCCTGCCTGG AGGGCAAGGG CTGCGAGCCG GACACGACAG CGACGTCCGG GCAGAATGAG
    AACGAGCGCT GCGGCACCGA CCTGGCCTAC GTCTACTTTG TCTCCTTCAT CTTCTTCTGC
    TCATTCCTGA TGCTCAACCT GTTCGTGGCT GTCATCATGG ACAACTTTGA GTACCTGACT
    CGAGACTCCT CCATCCTGGG ACCTCACCAT CTAGATGAAT TTGTCCGGAT CTGGGCAGAG
    TATGACAGAG CAGCGTGCGG CAGGATCTCG TATAAGGATA TGTACAAATT AGTACGGGTG
    ATCTCGCCTC CTCTGGGCCT AGGAAAGAAC TGCCCCTACA GGGTGGCGTG CAAGAGACTG
    GTGCTGATGA ACATGCCCGT GGCCGAGGAC ATGACGGTCC ATTTCACCTC CACCCTGATG
    GCGCTGATAC GCACCGCCCT GGACATCAAA ATTGCCAAAG GTGGTGCAGA TTGGCAGCAG
    TTAGACTCTG AGCTTCAAAA GGAGATCCTG ACCATTTGGC CTCACCTGTC CCAGAAGATG
    CTTGACCTGT TGGTACCCAT GCCAAAAACC TCTGACCTGA CTGTTGGCAA AATATATGCA
    GCCATGATGA TCATGGATTA CTACAAGCAG AGCAAGGCGA AGAAGCAGAG GCAGCAGCTG
    GAGGAGCAGA AAAATGCGCC CATGTTCCAG CGCATGGAGC CATCCTCTTT GCCACAGGAA
    ATCATCTCCA ACGCAAAGGC TCTGCCTTAC CTCCAGCAGG AGACCCTCAC AGGGCTGAGC
    AGCCGGAGCG GGTTCCCCTC GCTGAGCCCG CTCTCACCAC AGGAGATCTT CCAGCTGGCG
    TGCATGGATC CGGCCCACGG GCAGTTCCAG GAGCACCAGT CTCTGGAGCC AGAGGTTAGG
    GAGTTTCAAA GAGTGCAGCC CTCTAACCGT GGCAGCTACC TTCCCGTGGA CACTCAGGAC
    CACGCTGTCT CTGGGAGGGC CTCCTCCATG CCTCGTCTCA CTGTGGATCC CCAGGTGGTG
    ACGGACACCA GCTCGATGCG GCGGTCATTT TCCACCATCC GGGACAAACG CACCAACACC
    TCCTGGCTGG ATGAGTTCTC CATGGAGCGC AGCAGCGACA ACACCTACAA GTCCCGCCGG
    CGCAGCTACC ACTCCTCTCT CCAGCTCTCT GCCCGGCGCC TCAACGCTGA CTCAGGCCAC
    AGGTCTGAGG GGCACCGCTC GGGCGGCAGG GAGCGGGGCC GATCCAAAGA GCGCAAGCAC
    TTGCTGTCCC CTGACATCTC CCGCTGCAAC TCAGAGGAGA GGAGTCCCCA GGCACACGAT
    GAGTCCCCAG AGCGGCGGCG CGAGTCCCGG TCACCCAGCG AGGGCAGGTC GCAAACGCCC
    AACCGGCAGG GAACGGGCTC CCTGAGCGAA AGCTCCATCC CCTCCATCTC GGACACCAGC
    ACACCCCGGC GAGGCCGCCG CCAGCTCCCA CCTGTGCCCC CAAAACCGCG TCCCCTCCTC
    TCCTACGCCT CCATGCTGCG GCACGCTGCC GGAGACGCCT CACCACCTCC CGATGAGACC
    GAAGGGGGGT CCCCGCTCCT CTCCAAGGAG CCCAACGCCA CCGGCCTGAC CGAGTCTTCC
    AGCTCCCCGG CGGGGAAGCC GAGCCGGCCG TCCACCCCGC AGCGTTACAT CTCGGAGCCC
    TACCTGGCCC TGCACGACGA CTCGCACGCT TCGGACTGCG GCGAGGAGGA GACGCTCACC
    TTCGAGGCGG CCGTGGCCAC CAGTCTGGGT CGTTCCAACA CCATCGGCTC GGCCCCACCG
    CTGCGACACA GCTGGCAGAT GCCCAACGGG CACTACCGGA GACGGAGGCG AGGGGCCGGG
    CAGGGCGTGA TGTGCGGGGC CAGCATCGAT GTGCTCAGCG ACACCGAGGA AGATGACAAG
    TGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39015
    Clone ID Related Accession (Same CDS sequence) XM_015290481.1
    Accession Version XM_015290481.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAGC GGGAGCGGCG CCGGAGGCAC
    CACATGTCGG TGTGGGAGCA GCGCACCAGC CAGCTGCGCC GGCACATGCA GATGTCCAGC
    CAGGAGGGCA TCAATAAGGA TGAGCCACCC CTCATCAACC CACATGCCAG CATCTTCCGC
    CGCAAGAAGC CGGGGGACGG TGTTGCCTTG GAGAAGTGCG ATGAAGAGCA AGGCGGCAAG
    GGTGAGCGGC CGCCAGCTGA GGGTCCCGAG CAGCCGGCCC CAGGGACCAA CCCAGGCGGT
    GGGGAGGACA GGAAGAGCCC ATCGCCCCGT GCCAGGAGGG ACAAGGAGAT GTGGCAACAC
    AAAGCGTGCC ACGGGAACTG CGAGCCGGGT GAGGAAGGGG CAGGAGGTGG CATCGAGGAG
    AGGGCGCGGA TGAGACAGAG CCAGCGGCGC AGCCGGCATC GGAAAGCCAG GATGGAGGGG
    AAGGAGACGA CCGGAGCCCT GGAGAGCCGT GCGGGCAGCC AGGAGATGGG GCTGGAGGAG
    CCCTGCCCCG CAGAGGGGAT GCAGGATGGG GAGCGGCGTG GGGATTCTGC TGCCGCGCTG
    GACCTGATTC AGGGCGAGCT GGAGGCGAGC GGGGACCCCA CCAGGACTAA TGGGGTTCCC
    GCCGAGGAGG GAAGCCTCCC AGCATCAGCC CCTGAGCCTT CCCGGGGGAT GGAGGGCAGC
    CTGGGTGAGC AGGACTGCAG CAGCCCAGAC ACCAGTGAGC AGGCCCTGCT GGGGGGGGCG
    GCACTGGGGG CCAGCCGCAC CGTCAGCCAC AGCGAGCCTG ACCTGTCCTC CGTCACCGCC
    AACACTGAGA AGGCCACCGA GAGCACCACC ATCATGATTG ATGTCCAAGA CTCCACTGTG
    GTACAGATTA GCAACAAGAC TGATGGAGAA GCCAGCCCCT TGAAGGAGGC AGAGAGCAAA
    GAGGATGAGG AAGAGATGGA GAAGAAGAAG CGCAAGAAGG AGAAGAGCGA GACGGGCAAA
    GCGATGGTGC CACACAGCTC CATGTTCATC TTCAGCACCA CAAACCCGGT GCGCAGAGCC
    TGCCACTACA TTGTGAACCT GCGCTACTTT GAGATGTGCA TCCTCCTGGT CATCGCCGCC
    AGCAGCATCG CCCTCGCGGC CGAGGATCCC GTCCTCACCA ACTCCGACCG TAACAAAGTG
    CTGAGGTATT TTGATTACGT TTTCACCGGT GTCTTCACCT TCGAGATGGT TATCAAGATG
    ATCGACCAGG GCTTGATCCT GCAGGATGGC TCCTACTTCC GAGACCTCTG GAACATCCTG
    GATTTCATCG TGGTGGTGGG AGCTCTGGTG GCCTTTGCCT TGGCGAATGC TTTGGGGACC
    AACAAGGGTC GGGATATCAA GACTATCAAG TCTCTCCGTG TGCTGCGGGT CTTGAGGCCC
    CTGAAAACCA TCAAGCGGCT GCCGAAGCTC AAGGCTGTCT TTGACTGTGT TGTGACATCC
    CTCAAGAACG TCTTCAACAT CCTCATTGTC TACAAGCTCT TCATGTTCAT CTTTGCTGTC
    ATCGCCGTCC AGCTCTTCAA GGGGAAGTTT TTCTACTGCA CTGACAGCTC TAAGGACACA
    GAGAAGGACT GCATAGGGAA CTATGTGGAC CATGAGAAGA ACAAGATGGA GGTGAAGTGC
    CGGGAGTGGA AACGACATGA GTTTCACTAT GACAATATAA TCTGGGCGTT GCTCACCCTG
    TTCACAGTCT CCACCGGCGA GGGTTGGCCC CAGGTGCTGC AGCATTCGGT GGATGTGACG
    GAGGAGGACC GTGGGCCCAG CCGCAGTAAC CGCATGGAGA TGTCCATTTT TTACGTTGTC
    TATTTTGTGG TCTTCCCTTT CTTCTTCGTC AACATCTTTG TGGCGTTCAT CATCATCACC
    TTCCAAGAGC AAGGAGACAA AATGATGGAG GAGTGCAGTC TGGAGAAGAA CGAGAGGGCC
    TGTATTGACT TTGCCATCAG TGCCAAGCCC CTCACACGCT ACATGCCCCA GAACCGGCAC
    ACCTTCCAGT ATCGGGTCTG GCACTTTGTG GTCTCTCCAT CCTTTGAGTA TACCATCATG
    GCCATGATTG CTTTGAACAC TGTGGTGCTG ATGATGAAGT ACTACTCTGC TCCATACACC
    TATGAGCTGG CCCTGAAGTA CCTGAACATC GCTTTCACCA TGGTGTTCTC TTTGGAGTGC
    GTCCTTAAGA TCATTGCTTT TGGCTTCCTG AATTATTTCC GGGATACCTG GAATATCTTT
    GACTTCATCA CCGTCATCGG CAGCATCACA GAGATCATCC TGACAGACAC CAAGCTGGTG
    AACACCAGTA GCTTCAACAT GAGTTTCCTG AAACTCTTCC GGGCGGCTCG GCTCATCAAG
    CTGCTGCGCC AGGGTTACAC CATCCGCATC CTGCTGTGGA CGTTCGTGCA GTCCTTCAAG
    GCTCTCCCCT ATGTCTGCCT CCTAATCGCG ATGCTTTTCT TCATCTATGC CATCATTGGG
    ATGCAGGTTT TCGGCAACAT CAAACTGGAT GAGGAAAGCC ACATTAACCG GCACAACAAC
    TTCCGCAGCT TCCTGGGCTC CCTCATGCTC CTCTTCAGGA GTGCCACCGG TGAGGCATGG
    CAGGAGATCA TGCTCTCCTG CCTGGAGGGC AAGGGCTGCG AGCCGGACAC GACAGCGACG
    TCCGGGCAGA ATGAGAACGA GCGCTGCGGC ACCGACCTGG CCTACGTCTA CTTTGTCTCC
    TTCATCTTCT TCTGCTCATT CCTGATGCTC AACCTGTTCG TGGCTGTCAT CATGGACAAC
    TTTGAGTACC TGACTCGAGA CTCCTCCATC CTGGGACCTC ACCATCTAGA TGAATTTGTC
    CGGATCTGGG CAGAGTATGA CAGAGCAGCG TGTGGCCGAA TCCATTACAC TGAGATGTAT
    GAAATGCTGA CTCTTATGTC ACCACCGCTA GGCCTCGGCA AGAGATGTCC TTCCAAAGTG
    GCCTATAAGA GACTGGTGCT GATGAACATG CCCGTGGCCG AGGACATGAC GGTCCATTTC
    ACCTCCACCC TGATGGCGCT GATACGCACC GCCCTGGACA TCAAAATTGC CAAAGGTGGT
    GCAGATTGGC AGCAGTTAGA CTCTGAGCTT CAAAAGGAGA TCCTGACCAT TTGGCCTCAC
    CTGTCCCAGA AGATGCTTGA CCTGTTGGTA CCCATGCCAA AAACCTCTGA CCTGACTGTT
    GGCAAAATAT ATGCAGCCAT GATGATCATG GATTACTACA AGCAGAGCAA GGCGAAGAAG
    CAGAGGCAGC AGCTGGAGGA GCAGAAAAAT GCGCCCATGT TCCAGCGCAT GGAGCCATCC
    TCTTTGCCAC AGGAAATCAT CTCCAACGCA AAGGCTCTGC CTTACCTCCA GCAGGAGACC
    CTCACAGGGC TGAGCAGCCG GAGCGGGTTC CCCTCGCTGA GCCCGCTCTC ACCACAGGAG
    ATCTTCCAGC TGGCGTGCAT GGATCCGGCC CACGGGCAGT TCCAGGAGCA CCAGTCTCTG
    GAGCCAGAGG TTAGGGAGTT TCAAAGAGTG CAGCCCTCTA ACCGTGGCAG CTACCTTCCC
    GTGGACACTC AGGACCACGC TGTCTCTGGG AGGGCCTCCT CCATGCCTCG TCTCACTGTG
    GATCCCCAGG TGGTGACGGA CACCAGCTCG ATGCGGCGGT CATTTTCCAC CATCCGGGAC
    AAACGCACCA ACACCTCCTG GCTGGATGAG TTCTCCATGG AGCGCAGCAG CGACAACACC
    TACAAGTCCC GCCGGCGCAG CTACCACTCC TCTCTCCAGC TCTCTGCCCG GCGCCTCAAC
    GCTGACTCAG GCCACAGGTC TGAGGGGCAC CGCTCGGGCG GCAGGGAGCG GGGCCGATCC
    AAAGAGCGCA AGCACTTGCT GTCCCCTGAC ATCTCCCGCT GCAACTCAGA GGAGAGGAGT
    CCCCAGGCAC ACGATGAGTC CCCAGAGCGG CGGCGCGAGT CCCGGTCACC CAGCGAGGGC
    AGGTCGCAAA CGCCCAACCG GCAGGGAACG GGCTCCCTGA GCGAAAGCTC CATCCCCTCC
    ATCTCGGACA CCAGCACACC CCGGCGAGGC CGCCGCCAGC TCCCACCTGT GCCCCCAAAA
    CCGCGTCCCC TCCTCTCCTA CGCCTCCATG CTGCGGCACG CTGCCGGAGA CGCCTCACCA
    CCTCCCGATG AGACCGAAGG GGGGTCCCCG CTCCTCTCCA AGGAGCCCAA CGCCACCGGC
    CTGACCGAGT CTTCCAGCTC CCCGGCGGGG AAGCCGAGCC GGCCGTCCAC CCCGCAGCGT
    TACATCTCGG AGCCCTACCT GGCCCTGCAC GACGACTCGC ACGCTTCGGA CTGCGGCGAG
    GAGGAGACGC TCACCTTCGA GGCGGCCGTG GCCACCAGTC TGGGTCGTTC CAACACCATC
    GGCTCGGCCC CACCGCTGCG ACACAGCTGG CAGATGCCCA ACGGGCACTA CCGGAGACGG
    AGGCGAGGGG CCGGGCAGGG CGTGATGTGC GGGGCCAGCA TCGATGTGCT CAGCGACACC
    GAGGAAGATG ACAAGTGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145967.1
    CDS122..7342
    Translation

    Target ORF information:

    RefSeq Version XM_015290481.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290481.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAGC GGGAGCGGCG CCGGAGGCAC
    CACATGTCGG TGTGGGAGCA GCGCACCAGC CAGCTGCGCC GGCACATGCA GATGTCCAGC
    CAGGAGGGCA TCAATAAGGA TGAGCCACCC CTCATCAACC CACATGCCAG CATCTTCCGC
    CGCAAGAAGC CGGGGGACGG TGTTGCCTTG GAGAAGTGCG ATGAAGAGCA AGGCGGCAAG
    GGTGAGCGGC CGCCAGCTGA GGGTCCCGAG CAGCCGGCCC CAGGGACCAA CCCAGGCGGT
    GGGGAGGACA GGAAGAGCCC ATCGCCCCGT GCCAGGAGGG ACAAGGAGAT GTGGCAACAC
    AAAGCGTGCC ACGGGAACTG CGAGCCGGGT GAGGAAGGGG CAGGAGGTGG CATCGAGGAG
    AGGGCGCGGA TGAGACAGAG CCAGCGGCGC AGCCGGCATC GGAAAGCCAG GATGGAGGGG
    AAGGAGACGA CCGGAGCCCT GGAGAGCCGT GCGGGCAGCC AGGAGATGGG GCTGGAGGAG
    CCCTGCCCCG CAGAGGGGAT GCAGGATGGG GAGCGGCGTG GGGATTCTGC TGCCGCGCTG
    GACCTGATTC AGGGCGAGCT GGAGGCGAGC GGGGACCCCA CCAGGACTAA TGGGGTTCCC
    GCCGAGGAGG GAAGCCTCCC AGCATCAGCC CCTGAGCCTT CCCGGGGGAT GGAGGGCAGC
    CTGGGTGAGC AGGACTGCAG CAGCCCAGAC ACCAGTGAGC AGGCCCTGCT GGGGGGGGCG
    GCACTGGGGG CCAGCCGCAC CGTCAGCCAC AGCGAGCCTG ACCTGTCCTC CGTCACCGCC
    AACACTGAGA AGGCCACCGA GAGCACCACC ATCATGATTG ATGTCCAAGA CTCCACTGTG
    GTACAGATTA GCAACAAGAC TGATGGAGAA GCCAGCCCCT TGAAGGAGGC AGAGAGCAAA
    GAGGATGAGG AAGAGATGGA GAAGAAGAAG CGCAAGAAGG AGAAGAGCGA GACGGGCAAA
    GCGATGGTGC CACACAGCTC CATGTTCATC TTCAGCACCA CAAACCCGGT GCGCAGAGCC
    TGCCACTACA TTGTGAACCT GCGCTACTTT GAGATGTGCA TCCTCCTGGT CATCGCCGCC
    AGCAGCATCG CCCTCGCGGC CGAGGATCCC GTCCTCACCA ACTCCGACCG TAACAAAGTG
    CTGAGGTATT TTGATTACGT TTTCACCGGT GTCTTCACCT TCGAGATGGT TATCAAGATG
    ATCGACCAGG GCTTGATCCT GCAGGATGGC TCCTACTTCC GAGACCTCTG GAACATCCTG
    GATTTCATCG TGGTGGTGGG AGCTCTGGTG GCCTTTGCCT TGGCGAATGC TTTGGGGACC
    AACAAGGGTC GGGATATCAA GACTATCAAG TCTCTCCGTG TGCTGCGGGT CTTGAGGCCC
    CTGAAAACCA TCAAGCGGCT GCCGAAGCTC AAGGCTGTCT TTGACTGTGT TGTGACATCC
    CTCAAGAACG TCTTCAACAT CCTCATTGTC TACAAGCTCT TCATGTTCAT CTTTGCTGTC
    ATCGCCGTCC AGCTCTTCAA GGGGAAGTTT TTCTACTGCA CTGACAGCTC TAAGGACACA
    GAGAAGGACT GCATAGGGAA CTATGTGGAC CATGAGAAGA ACAAGATGGA GGTGAAGTGC
    CGGGAGTGGA AACGACATGA GTTTCACTAT GACAATATAA TCTGGGCGTT GCTCACCCTG
    TTCACAGTCT CCACCGGCGA GGGTTGGCCC CAGGTGCTGC AGCATTCGGT GGATGTGACG
    GAGGAGGACC GTGGGCCCAG CCGCAGTAAC CGCATGGAGA TGTCCATTTT TTACGTTGTC
    TATTTTGTGG TCTTCCCTTT CTTCTTCGTC AACATCTTTG TGGCGTTCAT CATCATCACC
    TTCCAAGAGC AAGGAGACAA AATGATGGAG GAGTGCAGTC TGGAGAAGAA CGAGAGGGCC
    TGTATTGACT TTGCCATCAG TGCCAAGCCC CTCACACGCT ACATGCCCCA GAACCGGCAC
    ACCTTCCAGT ATCGGGTCTG GCACTTTGTG GTCTCTCCAT CCTTTGAGTA TACCATCATG
    GCCATGATTG CTTTGAACAC TGTGGTGCTG ATGATGAAGT ACTACTCTGC TCCATACACC
    TATGAGCTGG CCCTGAAGTA CCTGAACATC GCTTTCACCA TGGTGTTCTC TTTGGAGTGC
    GTCCTTAAGA TCATTGCTTT TGGCTTCCTG AATTATTTCC GGGATACCTG GAATATCTTT
    GACTTCATCA CCGTCATCGG CAGCATCACA GAGATCATCC TGACAGACAC CAAGCTGGTG
    AACACCAGTA GCTTCAACAT GAGTTTCCTG AAACTCTTCC GGGCGGCTCG GCTCATCAAG
    CTGCTGCGCC AGGGTTACAC CATCCGCATC CTGCTGTGGA CGTTCGTGCA GTCCTTCAAG
    GCTCTCCCCT ATGTCTGCCT CCTAATCGCG ATGCTTTTCT TCATCTATGC CATCATTGGG
    ATGCAGGTTT TCGGCAACAT CAAACTGGAT GAGGAAAGCC ACATTAACCG GCACAACAAC
    TTCCGCAGCT TCCTGGGCTC CCTCATGCTC CTCTTCAGGA GTGCCACCGG TGAGGCATGG
    CAGGAGATCA TGCTCTCCTG CCTGGAGGGC AAGGGCTGCG AGCCGGACAC GACAGCGACG
    TCCGGGCAGA ATGAGAACGA GCGCTGCGGC ACCGACCTGG CCTACGTCTA CTTTGTCTCC
    TTCATCTTCT TCTGCTCATT CCTGATGCTC AACCTGTTCG TGGCTGTCAT CATGGACAAC
    TTTGAGTACC TGACTCGAGA CTCCTCCATC CTGGGACCTC ACCATCTAGA TGAATTTGTC
    CGGATCTGGG CAGAGTATGA CAGAGCAGCG TGTGGCCGAA TCCATTACAC TGAGATGTAT
    GAAATGCTGA CTCTTATGTC ACCACCGCTA GGCCTCGGCA AGAGATGTCC TTCCAAAGTG
    GCCTATAAGA GACTGGTGCT GATGAACATG CCCGTGGCCG AGGACATGAC GGTCCATTTC
    ACCTCCACCC TGATGGCGCT GATACGCACC GCCCTGGACA TCAAAATTGC CAAAGGTGGT
    GCAGATTGGC AGCAGTTAGA CTCTGAGCTT CAAAAGGAGA TCCTGACCAT TTGGCCTCAC
    CTGTCCCAGA AGATGCTTGA CCTGTTGGTA CCCATGCCAA AAACCTCTGA CCTGACTGTT
    GGCAAAATAT ATGCAGCCAT GATGATCATG GATTACTACA AGCAGAGCAA GGCGAAGAAG
    CAGAGGCAGC AGCTGGAGGA GCAGAAAAAT GCGCCCATGT TCCAGCGCAT GGAGCCATCC
    TCTTTGCCAC AGGAAATCAT CTCCAACGCA AAGGCTCTGC CTTACCTCCA GCAGGAGACC
    CTCACAGGGC TGAGCAGCCG GAGCGGGTTC CCCTCGCTGA GCCCGCTCTC ACCACAGGAG
    ATCTTCCAGC TGGCGTGCAT GGATCCGGCC CACGGGCAGT TCCAGGAGCA CCAGTCTCTG
    GAGCCAGAGG TTAGGGAGTT TCAAAGAGTG CAGCCCTCTA ACCGTGGCAG CTACCTTCCC
    GTGGACACTC AGGACCACGC TGTCTCTGGG AGGGCCTCCT CCATGCCTCG TCTCACTGTG
    GATCCCCAGG TGGTGACGGA CACCAGCTCG ATGCGGCGGT CATTTTCCAC CATCCGGGAC
    AAACGCACCA ACACCTCCTG GCTGGATGAG TTCTCCATGG AGCGCAGCAG CGACAACACC
    TACAAGTCCC GCCGGCGCAG CTACCACTCC TCTCTCCAGC TCTCTGCCCG GCGCCTCAAC
    GCTGACTCAG GCCACAGGTC TGAGGGGCAC CGCTCGGGCG GCAGGGAGCG GGGCCGATCC
    AAAGAGCGCA AGCACTTGCT GTCCCCTGAC ATCTCCCGCT GCAACTCAGA GGAGAGGAGT
    CCCCAGGCAC ACGATGAGTC CCCAGAGCGG CGGCGCGAGT CCCGGTCACC CAGCGAGGGC
    AGGTCGCAAA CGCCCAACCG GCAGGGAACG GGCTCCCTGA GCGAAAGCTC CATCCCCTCC
    ATCTCGGACA CCAGCACACC CCGGCGAGGC CGCCGCCAGC TCCCACCTGT GCCCCCAAAA
    CCGCGTCCCC TCCTCTCCTA CGCCTCCATG CTGCGGCACG CTGCCGGAGA CGCCTCACCA
    CCTCCCGATG AGACCGAAGG GGGGTCCCCG CTCCTCTCCA AGGAGCCCAA CGCCACCGGC
    CTGACCGAGT CTTCCAGCTC CCCGGCGGGG AAGCCGAGCC GGCCGTCCAC CCCGCAGCGT
    TACATCTCGG AGCCCTACCT GGCCCTGCAC GACGACTCGC ACGCTTCGGA CTGCGGCGAG
    GAGGAGACGC TCACCTTCGA GGCGGCCGTG GCCACCAGTC TGGGTCGTTC CAACACCATC
    GGCTCGGCCC CACCGCTGCG ACACAGCTGG CAGATGCCCA ACGGGCACTA CCGGAGACGG
    AGGCGAGGGG CCGGGCAGGG CGTGATGTGC GGGGCCAGCA TCGATGTGCT CAGCGACACC
    GAGGAAGATG ACAAGTGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39016
    Clone ID Related Accession (Same CDS sequence) XM_015290482.1
    Accession Version XM_015290482.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATCTTTGTGG CGTTCATCAT CATCACCTTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT
    GAGTACCTGA CTCGAGACTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGT GGCCGAATCC ATTACACTGA GATGTATGAA
    ATGCTGACTC TTATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCTTC CAAAGTGGCC
    TATAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGTG
    GTGACGGACA CCAGCTCGAT GCGGCGGTCA TTTTCCACCA TCCGGGACAA ACGCACCAAC
    ACCTCCTGGC TGGATGAGTT CTCCATGGAG CGCAGCAGCG ACAACACCTA CAAGTCCCGC
    CGGCGCAGCT ACCACTCCTC TCTCCAGCTC TCTGCCCGGC GCCTCAACGC TGACTCAGGC
    CACAGGTCTG AGGGGCACCG CTCGGGCGGC AGGGAGCGGG GCCGATCCAA AGAGCGCAAG
    CACTTGCTGT CCCCTGACAT CTCCCGCTGC AACTCAGAGG AGAGGAGTCC CCAGGCACAC
    GATGAGTCCC CAGAGCGGCG GCGCGAGTCC CGGTCACCCA GCGAGGGCAG GTCGCAAACG
    CCCAACCGGC AGGGAACGGG CTCCCTGAGC GAAAGCTCCA TCCCCTCCAT CTCGGACACC
    AGCACACCCC GGCGAGGCCG CCGCCAGCTC CCACCTGTGC CCCCAAAACC GCGTCCCCTC
    CTCTCCTACG CCTCCATGCT GCGGCACGCT GCCGGAGACG CCTCACCACC TCCCGATGAG
    ACCGAAGGGG GGTCCCCGCT CCTCTCCAAG GAGCCCAACG CCACCGGCCT GACCGAGTCT
    TCCAGCTCCC CGGCGGGGAA GCCGAGCCGG CCGTCCACCC CGCAGCGTTA CATCTCGGAG
    CCCTACCTGG CCCTGCACGA CGACTCGCAC GCTTCGGACT GCGGCGAGGA GGAGACGCTC
    ACCTTCGAGG CGGCCGTGGC CACCAGTCTG GGTCGTTCCA ACACCATCGG CTCGGCCCCA
    CCGCTGCGAC ACAGCTGGCA GATGCCCAAC GGGCACTACC GGAGACGGAG GCGAGGGGCC
    GGGCAGGGCG TGATGTGCGG GGCCAGCATC GATGTGCTCA GCGACACCGA GGAAGATGAC
    AAGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145968.1
    CDS121..7269
    Translation

    Target ORF information:

    RefSeq Version XM_015290482.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290482.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATCTTTGTGG CGTTCATCAT CATCACCTTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT
    GAGTACCTGA CTCGAGACTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGT GGCCGAATCC ATTACACTGA GATGTATGAA
    ATGCTGACTC TTATGTCACC ACCGCTAGGC CTCGGCAAGA GATGTCCTTC CAAAGTGGCC
    TATAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGTG
    GTGACGGACA CCAGCTCGAT GCGGCGGTCA TTTTCCACCA TCCGGGACAA ACGCACCAAC
    ACCTCCTGGC TGGATGAGTT CTCCATGGAG CGCAGCAGCG ACAACACCTA CAAGTCCCGC
    CGGCGCAGCT ACCACTCCTC TCTCCAGCTC TCTGCCCGGC GCCTCAACGC TGACTCAGGC
    CACAGGTCTG AGGGGCACCG CTCGGGCGGC AGGGAGCGGG GCCGATCCAA AGAGCGCAAG
    CACTTGCTGT CCCCTGACAT CTCCCGCTGC AACTCAGAGG AGAGGAGTCC CCAGGCACAC
    GATGAGTCCC CAGAGCGGCG GCGCGAGTCC CGGTCACCCA GCGAGGGCAG GTCGCAAACG
    CCCAACCGGC AGGGAACGGG CTCCCTGAGC GAAAGCTCCA TCCCCTCCAT CTCGGACACC
    AGCACACCCC GGCGAGGCCG CCGCCAGCTC CCACCTGTGC CCCCAAAACC GCGTCCCCTC
    CTCTCCTACG CCTCCATGCT GCGGCACGCT GCCGGAGACG CCTCACCACC TCCCGATGAG
    ACCGAAGGGG GGTCCCCGCT CCTCTCCAAG GAGCCCAACG CCACCGGCCT GACCGAGTCT
    TCCAGCTCCC CGGCGGGGAA GCCGAGCCGG CCGTCCACCC CGCAGCGTTA CATCTCGGAG
    CCCTACCTGG CCCTGCACGA CGACTCGCAC GCTTCGGACT GCGGCGAGGA GGAGACGCTC
    ACCTTCGAGG CGGCCGTGGC CACCAGTCTG GGTCGTTCCA ACACCATCGG CTCGGCCCCA
    CCGCTGCGAC ACAGCTGGCA GATGCCCAAC GGGCACTACC GGAGACGGAG GCGAGGGGCC
    GGGCAGGGCG TGATGTGCGG GGCCAGCATC GATGTGCTCA GCGACACCGA GGAAGATGAC
    AAGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39017
    Clone ID Related Accession (Same CDS sequence) XM_015290483.1
    Accession Version XM_015290483.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATCTTTGTGG CGTTCATCAT CATCACCTTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT
    GAGTACCTGA CTCGAGACTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGC GGCAGGATCT CGTATAAGGA TATGTACAAA
    TTAGTACGGG TGATCTCGCC TCCTCTGGGC CTAGGAAAGA ACTGCCCCTA CAGGGTGGCG
    TGCAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGTG
    GTGACGGACA CCAGCTCGAT GCGGCGGTCA TTTTCCACCA TCCGGGACAA ACGCACCAAC
    ACCTCCTGGC TGGATGAGTT CTCCATGGAG CGCAGCAGCG ACAACACCTA CAAGTCCCGC
    CGGCGCAGCT ACCACTCCTC TCTCCAGCTC TCTGCCCGGC GCCTCAACGC TGACTCAGGC
    CACAGGTCTG AGGGGCACCG CTCGGGCGGC AGGGAGCGGG GCCGATCCAA AGAGCGCAAG
    CACTTGCTGT CCCCTGACAT CTCCCGCTGC AACTCAGAGG AGAGGAGTCC CCAGGCACAC
    GATGAGTCCC CAGAGCGGCG GCGCGAGTCC CGGTCACCCA GCGAGGGCAG GTCGCAAACG
    CCCAACCGGC AGGGAACGGG CTCCCTGAGC GAAAGCTCCA TCCCCTCCAT CTCGGACACC
    AGCACACCCC GGCGAGGCCG CCGCCAGCTC CCACCTGTGC CCCCAAAACC GCGTCCCCTC
    CTCTCCTACG CCTCCATGCT GCGGCACGCT GCCGGAGACG CCTCACCACC TCCCGATGAG
    ACCGAAGGGG GGTCCCCGCT CCTCTCCAAG GAGCCCAACG CCACCGGCCT GACCGAGTCT
    TCCAGCTCCC CGGCGGGGAA GCCGAGCCGG CCGTCCACCC CGCAGCGTTA CATCTCGGAG
    CCCTACCTGG CCCTGCACGA CGACTCGCAC GCTTCGGACT GCGGCGAGGA GGAGACGCTC
    ACCTTCGAGG CGGCCGTGGC CACCAGTCTG GGTCGTTCCA ACACCATCGG CTCGGCCCCA
    CCGCTGCGAC ACAGCTGGCA GATGCCCAAC GGGCACTACC GGAGACGGAG GCGAGGGGCC
    GGGCAGGGCG TGATGTGCGG GGCCAGCATC GATGTGCTCA GCGACACCGA GGAAGATGAC
    AAGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145969.1
    CDS122..7270
    Translation

    Target ORF information:

    RefSeq Version XM_015290483.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290483.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAAA GAGACAGGCG CAGGAGACAC
    CACATGTCGA TGTGGGAGCC GCGCAGCAGC CACCTGCGGG AGCGGCGCCG GAGGCACCAC
    ATGTCGGTGT GGGAGCAGCG CACCAGCCAG CTGCGCCGGC ACATGCAGAT GTCCAGCCAG
    GAGGGCATCA ATAAGGATGA GCCACCCCTC ATCAACCCAC ATGCCAGCAT CTTCCGCCGC
    AAGAAGCCGG GGGACGGTGT TGCCTTGGAG AAGTGCGATG AAGAGCAAGG CGGCAAGGGT
    GAGCGGCCGC CAGCTGAGGG TCCCGAGCAG CCGGCCCCAG GGACCAACCC AGGCGGTGGG
    GAGGACAGGA AGAGCCCATC GCCCCGTGCC AGGAGGGACA AGGAGATGTG GCAACACAAA
    GCGTGCCACG GGAACTGCGA GCCGGGTGAG GAAGGGGCAG GAGGTGGCAT CGAGGAGAGG
    GCGCGGATGA GACAGAGCCA GCGGCGCAGC CGGCATCGGA AAGCCAGGAT GGAGGGGAAG
    GAGACGACCG GAGCCCTGGA GAGCCGTGCG GGCAGCCAGG AGATGGGGCT GGAGGAGCCC
    TGCCCCGCAG AGGGGATGCA GGATGGGGAG CGGCGTGGGG ATTCTGCTGC CGCGCTGGAC
    CTGATTCAGG GCGAGCTGGA GGCGAGCGGG GACCCCACCA GGACTAATGG GGTTCCCGCC
    GAGGAGGGAA GCCTCCCAGC ATCAGCCCCT GAGCCTTCCC GGGGGATGGA GGGCAGCCTG
    GGTGAGCAGG ACTGCAGCAG CCCAGACACC AGTGAGCAGG CCCTGCTGGG GGGGGCGGCA
    CTGGGGGCCA GCCGCACCGT CAGCCACAGC GAGCCTGACC TGTCCTCCGT CACCGCCAAC
    ACTGAGAAGG CCACCGAGAG CACCACCATC ATGATTGATG TCCAAGACTC CACTGTGGTA
    CAGATTAGCA ACAAGACTGA TGGAGAAGCC AGCCCCTTGA AGGAGGCAGA GAGCAAAGAG
    GATGAGGAAG AGATGGAGAA GAAGAAGCGC AAGAAGGAGA AGAGCGAGAC GGGCAAAGCG
    ATGGTGCCAC ACAGCTCCAT GTTCATCTTC AGCACCACAA ACCCGGTGCG CAGAGCCTGC
    CACTACATTG TGAACCTGCG CTACTTTGAG ATGTGCATCC TCCTGGTCAT CGCCGCCAGC
    AGCATCGCCC TCGCGGCCGA GGATCCCGTC CTCACCAACT CCGACCGTAA CAAAGTGCTG
    AGGTATTTTG ATTACGTTTT CACCGGTGTC TTCACCTTCG AGATGGTTAT CAAGATGATC
    GACCAGGGCT TGATCCTGCA GGATGGCTCC TACTTCCGAG ACCTCTGGAA CATCCTGGAT
    TTCATCGTGG TGGTGGGAGC TCTGGTGGCC TTTGCCTTGG CGAATGCTTT GGGGACCAAC
    AAGGGTCGGG ATATCAAGAC TATCAAGTCT CTCCGTGTGC TGCGGGTCTT GAGGCCCCTG
    AAAACCATCA AGCGGCTGCC GAAGCTCAAG GCTGTCTTTG ACTGTGTTGT GACATCCCTC
    AAGAACGTCT TCAACATCCT CATTGTCTAC AAGCTCTTCA TGTTCATCTT TGCTGTCATC
    GCCGTCCAGC TCTTCAAGGG GAAGTTTTTC TACTGCACTG ACAGCTCTAA GGACACAGAG
    AAGGACTGCA TAGGGAACTA TGTGGACCAT GAGAAGAACA AGATGGAGGT GAAGTGCCGG
    GAGTGGAAAC GACATGAGTT TCACTATGAC AATATAATCT GGGCGTTGCT CACCCTGTTC
    ACAGTCTCCA CCGGCGAGGG TTGGCCCCAG GTGCTGCAGC ATTCGGTGGA TGTGACGGAG
    GAGGACCGTG GGCCCAGCCG CAGTAACCGC ATGGAGATGT CCATTTTTTA CGTTGTCTAT
    TTTGTGGTCT TCCCTTTCTT CTTCGTCAAC ATCTTTGTGG CGTTCATCAT CATCACCTTC
    CAAGAGCAAG GAGACAAAAT GATGGAGGAG TGCAGTCTGG AGAAGAACGA GAGGGCCTGT
    ATTGACTTTG CCATCAGTGC CAAGCCCCTC ACACGCTACA TGCCCCAGAA CCGGCACACC
    TTCCAGTATC GGGTCTGGCA CTTTGTGGTC TCTCCATCCT TTGAGTATAC CATCATGGCC
    ATGATTGCTT TGAACACTGT GGTGCTGATG ATGAAGTACT ACTCTGCTCC ATACACCTAT
    GAGCTGGCCC TGAAGTACCT GAACATCGCT TTCACCATGG TGTTCTCTTT GGAGTGCGTC
    CTTAAGATCA TTGCTTTTGG CTTCCTGAAT TATTTCCGGG ATACCTGGAA TATCTTTGAC
    TTCATCACCG TCATCGGCAG CATCACAGAG ATCATCCTGA CAGACACCAA GCTGGTGAAC
    ACCAGTAGCT TCAACATGAG TTTCCTGAAA CTCTTCCGGG CGGCTCGGCT CATCAAGCTG
    CTGCGCCAGG GTTACACCAT CCGCATCCTG CTGTGGACGT TCGTGCAGTC CTTCAAGGCT
    CTCCCCTATG TCTGCCTCCT AATCGCGATG CTTTTCTTCA TCTATGCCAT CATTGGGATG
    CAGGTTTTCG GCAACATCAA ACTGGATGAG GAAAGCCACA TTAACCGGCA CAACAACTTC
    CGCAGCTTCC TGGGCTCCCT CATGCTCCTC TTCAGGAGTG CCACCGGTGA GGCATGGCAG
    GAGATCATGC TCTCCTGCCT GGAGGGCAAG GGCTGCGAGC CGGACACGAC AGCGACGTCC
    GGGCAGAATG AGAACGAGCG CTGCGGCACC GACCTGGCCT ACGTCTACTT TGTCTCCTTC
    ATCTTCTTCT GCTCATTCCT GATGCTCAAC CTGTTCGTGG CTGTCATCAT GGACAACTTT
    GAGTACCTGA CTCGAGACTC CTCCATCCTG GGACCTCACC ATCTAGATGA ATTTGTCCGG
    ATCTGGGCAG AGTATGACAG AGCAGCGTGC GGCAGGATCT CGTATAAGGA TATGTACAAA
    TTAGTACGGG TGATCTCGCC TCCTCTGGGC CTAGGAAAGA ACTGCCCCTA CAGGGTGGCG
    TGCAAGAGAC TGGTGCTGAT GAACATGCCC GTGGCCGAGG ACATGACGGT CCATTTCACC
    TCCACCCTGA TGGCGCTGAT ACGCACCGCC CTGGACATCA AAATTGCCAA AGGTGGTGCA
    GATTGGCAGC AGTTAGACTC TGAGCTTCAA AAGGAGATCC TGACCATTTG GCCTCACCTG
    TCCCAGAAGA TGCTTGACCT GTTGGTACCC ATGCCAAAAA CCTCTGACCT GACTGTTGGC
    AAAATATATG CAGCCATGAT GATCATGGAT TACTACAAGC AGAGCAAGGC GAAGAAGCAG
    AGGCAGCAGC TGGAGGAGCA GAAAAATGCG CCCATGTTCC AGCGCATGGA GCCATCCTCT
    TTGCCACAGG AAATCATCTC CAACGCAAAG GCTCTGCCTT ACCTCCAGCA GGAGACCCTC
    ACAGGGCTGA GCAGCCGGAG CGGGTTCCCC TCGCTGAGCC CGCTCTCACC ACAGGAGATC
    TTCCAGCTGG CGTGCATGGA TCCGGCCCAC GGGCAGTTCC AGGAGCACCA GTCTCTGGTG
    GTGACGGACA CCAGCTCGAT GCGGCGGTCA TTTTCCACCA TCCGGGACAA ACGCACCAAC
    ACCTCCTGGC TGGATGAGTT CTCCATGGAG CGCAGCAGCG ACAACACCTA CAAGTCCCGC
    CGGCGCAGCT ACCACTCCTC TCTCCAGCTC TCTGCCCGGC GCCTCAACGC TGACTCAGGC
    CACAGGTCTG AGGGGCACCG CTCGGGCGGC AGGGAGCGGG GCCGATCCAA AGAGCGCAAG
    CACTTGCTGT CCCCTGACAT CTCCCGCTGC AACTCAGAGG AGAGGAGTCC CCAGGCACAC
    GATGAGTCCC CAGAGCGGCG GCGCGAGTCC CGGTCACCCA GCGAGGGCAG GTCGCAAACG
    CCCAACCGGC AGGGAACGGG CTCCCTGAGC GAAAGCTCCA TCCCCTCCAT CTCGGACACC
    AGCACACCCC GGCGAGGCCG CCGCCAGCTC CCACCTGTGC CCCCAAAACC GCGTCCCCTC
    CTCTCCTACG CCTCCATGCT GCGGCACGCT GCCGGAGACG CCTCACCACC TCCCGATGAG
    ACCGAAGGGG GGTCCCCGCT CCTCTCCAAG GAGCCCAACG CCACCGGCCT GACCGAGTCT
    TCCAGCTCCC CGGCGGGGAA GCCGAGCCGG CCGTCCACCC CGCAGCGTTA CATCTCGGAG
    CCCTACCTGG CCCTGCACGA CGACTCGCAC GCTTCGGACT GCGGCGAGGA GGAGACGCTC
    ACCTTCGAGG CGGCCGTGGC CACCAGTCTG GGTCGTTCCA ACACCATCGG CTCGGCCCCA
    CCGCTGCGAC ACAGCTGGCA GATGCCCAAC GGGCACTACC GGAGACGGAG GCGAGGGGCC
    GGGCAGGGCG TGATGTGCGG GGCCAGCATC GATGTGCTCA GCGACACCGA GGAAGATGAC
    AAGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39018
    Clone ID Related Accession (Same CDS sequence) XM_015290484.1
    Accession Version XM_015290484.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6879bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCTCGCT TCGGGGAGGC GGTGGCTGGC AGGCTGGGCT CCGGGGATGG GGGCTCCGAG 
    CAGAACCGCA GCCGGCAAGG AGCCGCTGCT CCGGCGGGAG GGTCCCCGGG AGCCTTCAAG
    CAAACCAAAG CGCAGCGAGC CCGGACTATG GCTCTCTACA ACCCCATCCC CGTCCGGCAG
    AACTGCTTCA CCGTCAACAG ATCGTTGTTC CTCTTTGGGG AAGATAACAT AGTCAGGAAA
    TACGCCAAGA AGCTCATCGA CTGGCCTCCC TTCGAGTATA TGATCCTGGC AACCATAATT
    GCCAACTGCA TCGTGCTGGC CCTGGAGCAG CACCTCCCAG AGGACGACAA GACACCCATG
    TCACGGAGAC TGGAGAAGAC GGAGCCGTAC TTTATTGGGA TTTTCTGCTT TGAGGCTGGG
    ATTAAGATCG TGGCTCTGGG CTTCGTTTTC CACAAGGGCT CATACCTACG CAATGGCTGG
    AACGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTTG CCACCGCTGG AACACACTTC
    AACACGCACG TAGACCTGCG CACCCTGCGG GCCGTGCGTG TGCTCAGGCC TCTGAAGCTC
    GTCTCGGGCA TTCCCAGCCT TCAGATTGTG CTGAAATCCA TCATGAAGGC CATGGTACCT
    CTCCTCCAGA TTGGCTTGCT GCTCTTTTTC GCTATTCTCA TGTTTGCCAT CATCGGCCTG
    GAGTTTTACA GTGGGAAACT GCACCGAGCC TGCTACACAA ACAATTCAGG TGAGCTGGAG
    GAGCTGGACC CTCCCCACCC CTGCGGGGTG CAGGGCTGTC CGCCGGGCTA TGAGTGCCGG
    GAGTGGATTG GTCCCAATGA TGGGATCACG CAGTTCGACA ACATCCTCTT TGCTGTGCTG
    ACCGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACAG TTCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA CTGGCTATAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTCGTCCTCA ACCTTGTCCT GGGAGTGCTG TCAGGGGAGT TTGCCAAAGA ACGTGAGCGC
    GTGGAGAACC GCCGGGCCTT CATGAAGCTG CGGCGACAGC AGCAGATCGA GAGGGAGCTC
    AACGGCTACC GAGCCTGGAT AGACAAAGCG GAGGAAGTCA TGCTGGCTGA AGAGAACAAA
    AACGCTGGGA CCTCAGCCTT AGAAGTGCTC AGGAGAGCCA CCATCAAGAG GAACCGGACG
    GATGCCATGA ACCGTGACTC CAGCGATGAG CACTGCGTCG ATATCTCCTC CGTGGGCGAG
    CGGAGGCTGG CACAGAGGAG GCCTCTTCAT CCCACCTTGG GTAACCCCTT GAGTCGCTCC
    GGTCTCAAAG GAGTAGATGG AGCCTCCTAT TTACGGCACA AGGAGCGGCT CCTGCGCATT
    TCTGTTCGTC ACATGGTGAA ATCCCAGGTC TTCTACTGGA TTGTCTTGAG CCTGGTGGCT
    CTTAACACTG CCTGTGTGGC CATCGTCCAT CACAACCAGC CAGCCTGGCT CACCCACTTC
    CTCTACTATG CAGAGTTCCT GTTTCTTGGG CTCTTCCTCC TGGAGATGTC CTTGAAGATG
    TATGGGATGG GGCCACGCCT GTACTTCCAT TCTTCATTCA ACTGTTTCGA CTGTGGGGTC
    ACAGTGGGAA GCATCTTTGA AGTGGTCTGG GCCATCTTCC GACCAGGGAC CTCTTTTGGG
    ATCAGTGTCC TACGAGCCCT TCGTCTCCTG CGAATATTTA AAATAACCAA GTACTGGGCA
    TCCTTGAGGA ATTTGGTGGT CTCGCTGATG AGCTCTATGA AATCTATTAT CAGCCTGCTC
    TTCTTGCTCT TTCTCTTCAT TGTAGTCTTT GCCCTGCTGG GAATGCAGCT GTTTGGAGGC
    GGGTTTAATT TCATTGATGG GACACCATCG GCCAACTTTG ACACTTTCCC TGCAGCCATC
    ATGACGGTGT TCCAGATCTT GACGGGTGAG GACTGGAATG AGGTGATGTA CAATGGCATC
    CGCTCCCAGG GAGGTGTTCG CTCGGGCATG TGGTCCTCCA TCTATTTCAT CGTCCTCACC
    TTGTTTGGGA ACTATACTCT GCTGAATGTC TTCTTGGCCA TTGCAGTGGA CAACCTGGCT
    AATGCTCAGG AGCTCACCAA GGACGAGCAG GAGGAAGAGG AGGCCTTTAA CCAGAAGCAT
    GCCCTTCAGA AAGCCAAGGA AGTCAGCCCC ATGTCTGCCC CCAACATGCC TGCTATTGAA
    AGAGACAGGC GCAGGAGACA CCACATGTCG ATGTGGGAGC CGCGCAGCAG CCACCTGCGG
    GAGCGGCGCC GGAGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGCGCCGG
    CACATGCAGA TGTCCAGCCA GGAGGGCATC AATAAGGATG AGCCACCCCT CATCAACCCA
    CATGCCAGCA TCTTCCGCCG CAAGAAGCCG GGGGACGGTG TTGCCTTGGA GAAGTGCGAT
    GAAGAGCAAG GCGGCAAGGG TGAGCGGCCG CCAGCTGAGG GTCCCGAGCA GCCGGCCCCA
    GGGACCAACC CAGGCGGTGG GGAGGACAGG AAGAGCCCAT CGCCCCGTGC CAGGAGGGAC
    AAGGAGATGT GGCAACACAA AGCGTGCCAC GGGAACTGCG AGCCGGGTGA GGAAGGGGCA
    GGAGGTGGCA TCGAGGAGAG GGCGCGGATG AGACAGAGCC AGCGGCGCAG CCGGCATCGG
    AAAGCCAGGA TGGAGGGGAA GGAGACGACC GGAGCCCTGG AGAGCCGTGC GGGCAGCCAG
    GAGATGGGGC TGGAGGAGCC CTGCCCCGCA GAGGGGATGC AGGATGGGGA GCGGCGTGGG
    GATTCTGCTG CCGCGCTGGA CCTGATTCAG GGCGAGCTGG AGGCGAGCGG GGACCCCACC
    AGGACTAATG GGGTTCCCGC CGAGGAGGGA AGCCTCCCAG CATCAGCCCC TGAGCCTTCC
    CGGGGGATGG AGGGCAGCCT GGGTGAGCAG GACTGCAGCA GCCCAGACAC CAGTGAGCAG
    GCCCTGCTGG GGGGGGCGGC ACTGGGGGCC AGCCGCACCG TCAGCCACAG CGAGCCTGAC
    CTGTCCTCCG TCACCGCCAA CACTGAGAAG GCCACCGAGA GCACCACCAT CATGATTGAT
    GTCCAAGACT CCACTGTGGT ACAGATTAGC AACAAGACTG ATGGAGAAGC CAGCCCCTTG
    AAGGAGGCAG AGAGCAAAGA GGATGAGGAA GAGATGGAGA AGAAGAAGCG CAAGAAGGAG
    AAGAGCGAGA CGGGCAAAGC GATGGTGCCA CACAGCTCCA TGTTCATCTT CAGCACCACA
    AACCCGGTGC GCAGAGCCTG CCACTACATT GTGAACCTGC GCTACTTTGA GATGTGCATC
    CTCCTGGTCA TCGCCGCCAG CAGCATCGCC CTCGCGGCCG AGGATCCCGT CCTCACCAAC
    TCCGACCGTA ACAAAGTGCT GAGGTATTTT GATTACGTTT TCACCGGTGT CTTCACCTTC
    GAGATGGTTA TCAAGATGAT CGACCAGGGC TTGATCCTGC AGGATGGCTC CTACTTCCGA
    GACCTCTGGA ACATCCTGGA TTTCATCGTG GTGGTGGGAG CTCTGGTGGC CTTTGCCTTG
    GCGAATGCTT TGGGGACCAA CAAGGGTCGG GATATCAAGA CTATCAAGTC TCTCCGTGTG
    CTGCGGGTCT TGAGGCCCCT GAAAACCATC AAGCGGCTGC CGAAGCTCAA GGCTGTCTTT
    GACTGTGTTG TGACATCCCT CAAGAACGTC TTCAACATCC TCATTGTCTA CAAGCTCTTC
    ATGTTCATCT TTGCTGTCAT CGCCGTCCAG CTCTTCAAGG GGAAGTTTTT CTACTGCACT
    GACAGCTCTA AGGACACAGA GAAGGACTGC ATAGGGAACT ATGTGGACCA TGAGAAGAAC
    AAGATGGAGG TGAAGTGCCG GGAGTGGAAA CGACATGAGT TTCACTATGA CAATATAATC
    TGGGCGTTGC TCACCCTGTT CACAGTCTCC ACCGGCGAGG GTTGGCCCCA GGTGCTGCAG
    CATTCGGTGG ATGTGACGGA GGAGGACCGT GGGCCCAGCC GCAGTAACCG CATGGAGATG
    TCCATTTTTT ACGTTGTCTA TTTTGTGGTC TTCCCTTTCT TCTTCGTCAA CATCTTTGTG
    GCGTTCATCA TCATCACCTT CCAAGAGCAA GGAGACAAAA TGATGGAGGA GTGCAGTCTG
    GAGAAGAACG AGAGGGCCTG TATTGACTTT GCCATCAGTG CCAAGCCCCT CACACGCTAC
    ATGCCCCAGA ACCGGCACAC CTTCCAGTAT CGGGTCTGGC ACTTTGTGGT CTCTCCATCC
    TTTGAGTATA CCATCATGGC CATGATTGCT TTGAACACTG TGGTGCTGAT GATGAAGTAC
    TACTCTGCTC CATACACCTA TGAGCTGGCC CTGAAGTACC TGAACATCGC TTTCACCATG
    GTGTTCTCTT TGGAGTGCGT CCTTAAGATC ATTGCTTTTG GCTTCCTGAA TTATTTCCGG
    GATACCTGGA ATATCTTTGA CTTCATCACC GTCATCGGCA GCATCACAGA GATCATCCTG
    ACAGACACCA AGCTGGTGAA CACCAGTAGC TTCAACATGA GTTTCCTGAA ACTCTTCCGG
    GCGGCTCGGC TCATCAAGCT GCTGCGCCAG GGTTACACCA TCCGCATCCT GCTGTGGACG
    TTCGTGCAGT CCTTCAAGGC TCTCCCCTAT GTCTGCCTCC TAATCGCGAT GCTTTTCTTC
    ATCTATGCCA TCATTGGGAT GCAGGTTTTC GGCAACATCA AACTGGATGA GGAAAGCCAC
    ATTAACCGGC ACAACAACTT CCGCAGCTTC CTGGGCTCCC TCATGCTCCT CTTCAGGAGT
    GCCACCGGTG AGGCATGGCA GGAGATCATG CTCTCCTGCC TGGAGGGCAA GGGCTGCGAG
    CCGGACACGA CAGCGACGTC CGGGCAGAAT GAGAACGAGC GCTGCGGCAC CGACCTGGCC
    TACGTCTACT TTGTCTCCTT CATCTTCTTC TGCTCATTCC TGATGCTCAA CCTGTTCGTG
    GCTGTCATCA TGGACAACTT TGAGTACCTG ACTCGAGACT CCTCCATCCT GGGACCTCAC
    CATCTAGATG AATTTGTCCG GATCTGGGCA GAGTATGACA GAGCAGCGTG TGGCCGAATC
    CATTACACTG AGATGTATGA AATGCTGACT CTTATGTCAC CACCGCTAGG CCTCGGCAAG
    AGATGTCCTT CCAAAGTGGC CTATAAGAGA CTGGTGCTGA TGAACATGCC CGTGGCCGAG
    GACATGACGG TCCATTTCAC CTCCACCCTG ATGGCGCTGA TACGCACCGC CCTGGACATC
    AAAATTGCCA AAGGTGGTGC AGATTGGCAG CAGTTAGACT CTGAGCTTCA AAAGGAGATC
    CTGACCATTT GGCCTCACCT GTCCCAGAAG ATGCTTGACC TGTTGGTACC CATGCCAAAA
    ACCTCTGACC TGACTGTTGG CAAAATATAT GCAGCCATGA TGATCATGGA TTACTACAAG
    CAGAGCAAGG CGAAGAAGCA GAGGCAGCAG CTGGAGGAGC AGAAAAATGC GCCCATGTTC
    CAGCGCATGG AGCCATCCTC TTTGCCACAG GAAATCATCT CCAACGCAAA GGCTCTGCCT
    TACCTCCAGC AGGAGACCCT CACAGGGCTG AGCAGCCGGA GCGGGTTCCC CTCGCTGAGC
    CCGCTCTCAC CACAGGAGAT CTTCCAGCTG GCGTGCATGG ATCCGGCCCA CGGGCAGTTC
    CAGGAGCACC AGTCTCTGGA GCCAGAGGTT AGGGAGTTTC AAAGAGTGCA GCCCTCTAAC
    CGTGGCAGCT ACCTTCCCGT GGACACTCAG GACCACGCTG TCTCTGGGAG GGCCTCCTCC
    ATGCCTCGTC TCACTGTGGA TCCCCAGGTG GTGACGGACA CCAGCTCGAT GCGGCGGTCA
    TTTTCCACCA TCCGGGACAA ACGCACCAAC ACCTCCTGGC TGGATGAGTT CTCCATGGAG
    CGCAGCAGCG ACAACACCTA CAAGTCCCGC CGGCGCAGCT ACCACTCCTC TCTCCAGCTC
    TCTGCCCGGC GCCTCAACGC TGACTCAGGC CACAGGTCTG AGGGGCACCG CTCGGGCGGC
    AGGGAGCGGG GCCGATCCAA AGAGCGCAAG CACTTGCTGT CCCCTGACAT CTCCCGCTGC
    AACTCAGAGG AGAGGAGTCC CCAGGCACAC GATGAGTCCC CAGAGCGGCG GCGCGAGTCC
    CGGTCACCCA GCGAGGGCAG GTCGCAAACG CCCAACCGGC AGGGAACGGG CTCCCTGAGC
    GAAAGCTCCA TCCCCTCCAT CTCGGACACC AGCACACCCC GGCGAGGCCG CCGCCAGCTC
    CCACCTGTGC CCCCAAAACC GCGTCCCCTC CTCTCCTACG CCTCCATGCT GCGGCACGCT
    GCCGGAGACG CCTCACCACC TCCCGATGAG ACCGAAGGGG GGTCCCCGCT CCTCTCCAAG
    GAGCCCAACG CCACCGGCCT GACCGAGTCT TCCAGCTCCC CGGCGGGGAA GCCGAGCCGG
    CCGTCCACCC CGCAGCGTTA CATCTCGGAG CCCTACCTGG CCCTGCACGA CGACTCGCAC
    GCTTCGGACT GCGGCGAGGA GGAGACGCTC ACCTTCGAGG CGGCCGTGGC CACCAGTCTG
    GGTCGTTCCA ACACCATCGG CTCGGCCCCA CCGCTGCGAC ACAGCTGGCA GATGCCCAAC
    GGGCACTACC GGAGACGGAG GCGAGGGGCC GGGCAGGGCG TGATGTGCGG GGCCAGCATC
    GATGTGCTCA GCGACACCGA GGAAGATGAC AAGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145970.1
    CDS451..7329
    Translation

    Target ORF information:

    RefSeq Version XM_015290484.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290484.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCTCGCT TCGGGGAGGC GGTGGCTGGC AGGCTGGGCT CCGGGGATGG GGGCTCCGAG 
    CAGAACCGCA GCCGGCAAGG AGCCGCTGCT CCGGCGGGAG GGTCCCCGGG AGCCTTCAAG
    CAAACCAAAG CGCAGCGAGC CCGGACTATG GCTCTCTACA ACCCCATCCC CGTCCGGCAG
    AACTGCTTCA CCGTCAACAG ATCGTTGTTC CTCTTTGGGG AAGATAACAT AGTCAGGAAA
    TACGCCAAGA AGCTCATCGA CTGGCCTCCC TTCGAGTATA TGATCCTGGC AACCATAATT
    GCCAACTGCA TCGTGCTGGC CCTGGAGCAG CACCTCCCAG AGGACGACAA GACACCCATG
    TCACGGAGAC TGGAGAAGAC GGAGCCGTAC TTTATTGGGA TTTTCTGCTT TGAGGCTGGG
    ATTAAGATCG TGGCTCTGGG CTTCGTTTTC CACAAGGGCT CATACCTACG CAATGGCTGG
    AACGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTTG CCACCGCTGG AACACACTTC
    AACACGCACG TAGACCTGCG CACCCTGCGG GCCGTGCGTG TGCTCAGGCC TCTGAAGCTC
    GTCTCGGGCA TTCCCAGCCT TCAGATTGTG CTGAAATCCA TCATGAAGGC CATGGTACCT
    CTCCTCCAGA TTGGCTTGCT GCTCTTTTTC GCTATTCTCA TGTTTGCCAT CATCGGCCTG
    GAGTTTTACA GTGGGAAACT GCACCGAGCC TGCTACACAA ACAATTCAGG TGAGCTGGAG
    GAGCTGGACC CTCCCCACCC CTGCGGGGTG CAGGGCTGTC CGCCGGGCTA TGAGTGCCGG
    GAGTGGATTG GTCCCAATGA TGGGATCACG CAGTTCGACA ACATCCTCTT TGCTGTGCTG
    ACCGTCTTCC AGTGCATCAC CATGGAAGGG TGGACCACAG TTCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA CTGGCTATAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTCGTCCTCA ACCTTGTCCT GGGAGTGCTG TCAGGGGAGT TTGCCAAAGA ACGTGAGCGC
    GTGGAGAACC GCCGGGCCTT CATGAAGCTG CGGCGACAGC AGCAGATCGA GAGGGAGCTC
    AACGGCTACC GAGCCTGGAT AGACAAAGCG GAGGAAGTCA TGCTGGCTGA AGAGAACAAA
    AACGCTGGGA CCTCAGCCTT AGAAGTGCTC AGGAGAGCCA CCATCAAGAG GAACCGGACG
    GATGCCATGA ACCGTGACTC CAGCGATGAG CACTGCGTCG ATATCTCCTC CGTGGGCGAG
    CGGAGGCTGG CACAGAGGAG GCCTCTTCAT CCCACCTTGG GTAACCCCTT GAGTCGCTCC
    GGTCTCAAAG GAGTAGATGG AGCCTCCTAT TTACGGCACA AGGAGCGGCT CCTGCGCATT
    TCTGTTCGTC ACATGGTGAA ATCCCAGGTC TTCTACTGGA TTGTCTTGAG CCTGGTGGCT
    CTTAACACTG CCTGTGTGGC CATCGTCCAT CACAACCAGC CAGCCTGGCT CACCCACTTC
    CTCTACTATG CAGAGTTCCT GTTTCTTGGG CTCTTCCTCC TGGAGATGTC CTTGAAGATG
    TATGGGATGG GGCCACGCCT GTACTTCCAT TCTTCATTCA ACTGTTTCGA CTGTGGGGTC
    ACAGTGGGAA GCATCTTTGA AGTGGTCTGG GCCATCTTCC GACCAGGGAC CTCTTTTGGG
    ATCAGTGTCC TACGAGCCCT TCGTCTCCTG CGAATATTTA AAATAACCAA GTACTGGGCA
    TCCTTGAGGA ATTTGGTGGT CTCGCTGATG AGCTCTATGA AATCTATTAT CAGCCTGCTC
    TTCTTGCTCT TTCTCTTCAT TGTAGTCTTT GCCCTGCTGG GAATGCAGCT GTTTGGAGGC
    GGGTTTAATT TCATTGATGG GACACCATCG GCCAACTTTG ACACTTTCCC TGCAGCCATC
    ATGACGGTGT TCCAGATCTT GACGGGTGAG GACTGGAATG AGGTGATGTA CAATGGCATC
    CGCTCCCAGG GAGGTGTTCG CTCGGGCATG TGGTCCTCCA TCTATTTCAT CGTCCTCACC
    TTGTTTGGGA ACTATACTCT GCTGAATGTC TTCTTGGCCA TTGCAGTGGA CAACCTGGCT
    AATGCTCAGG AGCTCACCAA GGACGAGCAG GAGGAAGAGG AGGCCTTTAA CCAGAAGCAT
    GCCCTTCAGA AAGCCAAGGA AGTCAGCCCC ATGTCTGCCC CCAACATGCC TGCTATTGAA
    AGAGACAGGC GCAGGAGACA CCACATGTCG ATGTGGGAGC CGCGCAGCAG CCACCTGCGG
    GAGCGGCGCC GGAGGCACCA CATGTCGGTG TGGGAGCAGC GCACCAGCCA GCTGCGCCGG
    CACATGCAGA TGTCCAGCCA GGAGGGCATC AATAAGGATG AGCCACCCCT CATCAACCCA
    CATGCCAGCA TCTTCCGCCG CAAGAAGCCG GGGGACGGTG TTGCCTTGGA GAAGTGCGAT
    GAAGAGCAAG GCGGCAAGGG TGAGCGGCCG CCAGCTGAGG GTCCCGAGCA GCCGGCCCCA
    GGGACCAACC CAGGCGGTGG GGAGGACAGG AAGAGCCCAT CGCCCCGTGC CAGGAGGGAC
    AAGGAGATGT GGCAACACAA AGCGTGCCAC GGGAACTGCG AGCCGGGTGA GGAAGGGGCA
    GGAGGTGGCA TCGAGGAGAG GGCGCGGATG AGACAGAGCC AGCGGCGCAG CCGGCATCGG
    AAAGCCAGGA TGGAGGGGAA GGAGACGACC GGAGCCCTGG AGAGCCGTGC GGGCAGCCAG
    GAGATGGGGC TGGAGGAGCC CTGCCCCGCA GAGGGGATGC AGGATGGGGA GCGGCGTGGG
    GATTCTGCTG CCGCGCTGGA CCTGATTCAG GGCGAGCTGG AGGCGAGCGG GGACCCCACC
    AGGACTAATG GGGTTCCCGC CGAGGAGGGA AGCCTCCCAG CATCAGCCCC TGAGCCTTCC
    CGGGGGATGG AGGGCAGCCT GGGTGAGCAG GACTGCAGCA GCCCAGACAC CAGTGAGCAG
    GCCCTGCTGG GGGGGGCGGC ACTGGGGGCC AGCCGCACCG TCAGCCACAG CGAGCCTGAC
    CTGTCCTCCG TCACCGCCAA CACTGAGAAG GCCACCGAGA GCACCACCAT CATGATTGAT
    GTCCAAGACT CCACTGTGGT ACAGATTAGC AACAAGACTG ATGGAGAAGC CAGCCCCTTG
    AAGGAGGCAG AGAGCAAAGA GGATGAGGAA GAGATGGAGA AGAAGAAGCG CAAGAAGGAG
    AAGAGCGAGA CGGGCAAAGC GATGGTGCCA CACAGCTCCA TGTTCATCTT CAGCACCACA
    AACCCGGTGC GCAGAGCCTG CCACTACATT GTGAACCTGC GCTACTTTGA GATGTGCATC
    CTCCTGGTCA TCGCCGCCAG CAGCATCGCC CTCGCGGCCG AGGATCCCGT CCTCACCAAC
    TCCGACCGTA ACAAAGTGCT GAGGTATTTT GATTACGTTT TCACCGGTGT CTTCACCTTC
    GAGATGGTTA TCAAGATGAT CGACCAGGGC TTGATCCTGC AGGATGGCTC CTACTTCCGA
    GACCTCTGGA ACATCCTGGA TTTCATCGTG GTGGTGGGAG CTCTGGTGGC CTTTGCCTTG
    GCGAATGCTT TGGGGACCAA CAAGGGTCGG GATATCAAGA CTATCAAGTC TCTCCGTGTG
    CTGCGGGTCT TGAGGCCCCT GAAAACCATC AAGCGGCTGC CGAAGCTCAA GGCTGTCTTT
    GACTGTGTTG TGACATCCCT CAAGAACGTC TTCAACATCC TCATTGTCTA CAAGCTCTTC
    ATGTTCATCT TTGCTGTCAT CGCCGTCCAG CTCTTCAAGG GGAAGTTTTT CTACTGCACT
    GACAGCTCTA AGGACACAGA GAAGGACTGC ATAGGGAACT ATGTGGACCA TGAGAAGAAC
    AAGATGGAGG TGAAGTGCCG GGAGTGGAAA CGACATGAGT TTCACTATGA CAATATAATC
    TGGGCGTTGC TCACCCTGTT CACAGTCTCC ACCGGCGAGG GTTGGCCCCA GGTGCTGCAG
    CATTCGGTGG ATGTGACGGA GGAGGACCGT GGGCCCAGCC GCAGTAACCG CATGGAGATG
    TCCATTTTTT ACGTTGTCTA TTTTGTGGTC TTCCCTTTCT TCTTCGTCAA CATCTTTGTG
    GCGTTCATCA TCATCACCTT CCAAGAGCAA GGAGACAAAA TGATGGAGGA GTGCAGTCTG
    GAGAAGAACG AGAGGGCCTG TATTGACTTT GCCATCAGTG CCAAGCCCCT CACACGCTAC
    ATGCCCCAGA ACCGGCACAC CTTCCAGTAT CGGGTCTGGC ACTTTGTGGT CTCTCCATCC
    TTTGAGTATA CCATCATGGC CATGATTGCT TTGAACACTG TGGTGCTGAT GATGAAGTAC
    TACTCTGCTC CATACACCTA TGAGCTGGCC CTGAAGTACC TGAACATCGC TTTCACCATG
    GTGTTCTCTT TGGAGTGCGT CCTTAAGATC ATTGCTTTTG GCTTCCTGAA TTATTTCCGG
    GATACCTGGA ATATCTTTGA CTTCATCACC GTCATCGGCA GCATCACAGA GATCATCCTG
    ACAGACACCA AGCTGGTGAA CACCAGTAGC TTCAACATGA GTTTCCTGAA ACTCTTCCGG
    GCGGCTCGGC TCATCAAGCT GCTGCGCCAG GGTTACACCA TCCGCATCCT GCTGTGGACG
    TTCGTGCAGT CCTTCAAGGC TCTCCCCTAT GTCTGCCTCC TAATCGCGAT GCTTTTCTTC
    ATCTATGCCA TCATTGGGAT GCAGGTTTTC GGCAACATCA AACTGGATGA GGAAAGCCAC
    ATTAACCGGC ACAACAACTT CCGCAGCTTC CTGGGCTCCC TCATGCTCCT CTTCAGGAGT
    GCCACCGGTG AGGCATGGCA GGAGATCATG CTCTCCTGCC TGGAGGGCAA GGGCTGCGAG
    CCGGACACGA CAGCGACGTC CGGGCAGAAT GAGAACGAGC GCTGCGGCAC CGACCTGGCC
    TACGTCTACT TTGTCTCCTT CATCTTCTTC TGCTCATTCC TGATGCTCAA CCTGTTCGTG
    GCTGTCATCA TGGACAACTT TGAGTACCTG ACTCGAGACT CCTCCATCCT GGGACCTCAC
    CATCTAGATG AATTTGTCCG GATCTGGGCA GAGTATGACA GAGCAGCGTG TGGCCGAATC
    CATTACACTG AGATGTATGA AATGCTGACT CTTATGTCAC CACCGCTAGG CCTCGGCAAG
    AGATGTCCTT CCAAAGTGGC CTATAAGAGA CTGGTGCTGA TGAACATGCC CGTGGCCGAG
    GACATGACGG TCCATTTCAC CTCCACCCTG ATGGCGCTGA TACGCACCGC CCTGGACATC
    AAAATTGCCA AAGGTGGTGC AGATTGGCAG CAGTTAGACT CTGAGCTTCA AAAGGAGATC
    CTGACCATTT GGCCTCACCT GTCCCAGAAG ATGCTTGACC TGTTGGTACC CATGCCAAAA
    ACCTCTGACC TGACTGTTGG CAAAATATAT GCAGCCATGA TGATCATGGA TTACTACAAG
    CAGAGCAAGG CGAAGAAGCA GAGGCAGCAG CTGGAGGAGC AGAAAAATGC GCCCATGTTC
    CAGCGCATGG AGCCATCCTC TTTGCCACAG GAAATCATCT CCAACGCAAA GGCTCTGCCT
    TACCTCCAGC AGGAGACCCT CACAGGGCTG AGCAGCCGGA GCGGGTTCCC CTCGCTGAGC
    CCGCTCTCAC CACAGGAGAT CTTCCAGCTG GCGTGCATGG ATCCGGCCCA CGGGCAGTTC
    CAGGAGCACC AGTCTCTGGA GCCAGAGGTT AGGGAGTTTC AAAGAGTGCA GCCCTCTAAC
    CGTGGCAGCT ACCTTCCCGT GGACACTCAG GACCACGCTG TCTCTGGGAG GGCCTCCTCC
    ATGCCTCGTC TCACTGTGGA TCCCCAGGTG GTGACGGACA CCAGCTCGAT GCGGCGGTCA
    TTTTCCACCA TCCGGGACAA ACGCACCAAC ACCTCCTGGC TGGATGAGTT CTCCATGGAG
    CGCAGCAGCG ACAACACCTA CAAGTCCCGC CGGCGCAGCT ACCACTCCTC TCTCCAGCTC
    TCTGCCCGGC GCCTCAACGC TGACTCAGGC CACAGGTCTG AGGGGCACCG CTCGGGCGGC
    AGGGAGCGGG GCCGATCCAA AGAGCGCAAG CACTTGCTGT CCCCTGACAT CTCCCGCTGC
    AACTCAGAGG AGAGGAGTCC CCAGGCACAC GATGAGTCCC CAGAGCGGCG GCGCGAGTCC
    CGGTCACCCA GCGAGGGCAG GTCGCAAACG CCCAACCGGC AGGGAACGGG CTCCCTGAGC
    GAAAGCTCCA TCCCCTCCAT CTCGGACACC AGCACACCCC GGCGAGGCCG CCGCCAGCTC
    CCACCTGTGC CCCCAAAACC GCGTCCCCTC CTCTCCTACG CCTCCATGCT GCGGCACGCT
    GCCGGAGACG CCTCACCACC TCCCGATGAG ACCGAAGGGG GGTCCCCGCT CCTCTCCAAG
    GAGCCCAACG CCACCGGCCT GACCGAGTCT TCCAGCTCCC CGGCGGGGAA GCCGAGCCGG
    CCGTCCACCC CGCAGCGTTA CATCTCGGAG CCCTACCTGG CCCTGCACGA CGACTCGCAC
    GCTTCGGACT GCGGCGAGGA GGAGACGCTC ACCTTCGAGG CGGCCGTGGC CACCAGTCTG
    GGTCGTTCCA ACACCATCGG CTCGGCCCCA CCGCTGCGAC ACAGCTGGCA GATGCCCAAC
    GGGCACTACC GGAGACGGAG GCGAGGGGCC GGGCAGGGCG TGATGTGCGG GGCCAGCATC
    GATGTGCTCA GCGACACCGA GGAAGATGAC AAGTGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39021
    Clone ID Related Accession (Same CDS sequence) XM_015290487.1
    Accession Version XM_015290487.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAGC GGGAGCGGCG CCGGAGGCAC
    CACATGTCGG TGTGGGAGCA GCGCACCAGC CAGCTGCGCC GGCACATGCA GATGTCCAGC
    CAGGAGGGCA TCAATAAGGA TGAGCCACCC CTCATCAACC CACATGCCAG CATCTTCCGC
    CGCAAGAAGC CGGGGGACGG TGTTGCCTTG GAGAAGTGCG ATGAAGAGCA AGGCGGCAAG
    GGTGAGCGGC CGCCAGCTGA GGGTCCCGAG CAGCCGGCCC CAGGGACCAA CCCAGGCGGT
    GGGGAGGACA GGAAGAGCCC ATCGCCCCGT GCCAGGAGGG ACAAGGAGAT GTGGCAACAC
    AAAGCGTGCC ACGGGAACTG CGAGCCGGGT GAGGAAGGGG CAGGAGGTGG CATCGAGGAG
    AGGGCGCGGA TGAGACAGAG CCAGCGGCGC AGCCGGCATC GGAAAGCCAG GATGGAGGGG
    AAGGAGACGA CCGGAGCCCT GGAGAGCCGT GCGGGCAGCC AGGAGATGGG GCTGGAGGAG
    CCCTGCCCCG CAGAGGGGAT GCAGGATGGG GAGCGGCGTG GGGATTCTGC TGCCGCGCTG
    GACCTGATTC AGGGCGAGCT GGAGGCGAGC GGGGACCCCA CCAGGACTAA TGGGGTTCCC
    GCCGAGGAGG GAAGCCTCCC AGCATCAGCC CCTGAGCCTT CCCGGGGGAT GGAGGGCAGC
    CTGGGTGAGC AGGACTGCAG CAGCCCAGAC ACCAGTGAGC AGGCCCTGCT GGGGGGGGCG
    GCACTGGGGG CCAGCCGCAC CGTCAGCCAC AGCGAGCCTG ACCTGTCCTC CGTCACCGCC
    AACACTGAGA AGGCCACCGA GAGCACCACC ATCATGATTG ATGTCCAAGA CTCCACTGTG
    GTACAGATTA GCAACAAGAC TGATGGAGAA GCCAGCCCCT TGAAGGAGGC AGAGAGCAAA
    GAGGATGAGG AAGAGATGGA GAAGAAGAAG CGCAAGAAGG AGAAGAGCGA GACGGGCAAA
    GCGATGGTGC CACACAGCTC CATGTTCATC TTCAGCACCA CAAACCCGGT GCGCAGAGCC
    TGCCACTACA TTGTGAACCT GCGCTACTTT GAGATGTGCA TCCTCCTGGT CATCGCCGCC
    AGCAGCATCG CCCTCGCGGC CGAGGATCCC GTCCTCACCA ACTCCGACCG TAACAAAGTG
    CTGAGGTATT TTGATTACGT TTTCACCGGT GTCTTCACCT TCGAGATGGT TATCAAGATG
    ATCGACCAGG GCTTGATCCT GCAGGATGGC TCCTACTTCC GAGACCTCTG GAACATCCTG
    GATTTCATCG TGGTGGTGGG AGCTCTGGTG GCCTTTGCCT TGGCGAATGC TTTGGGGACC
    AACAAGGGTC GGGATATCAA GACTATCAAG TCTCTCCGTG TGCTGCGGGT CTTGAGGCCC
    CTGAAAACCA TCAAGCGGCT GCCGAAGCTC AAGGCTGTCT TTGACTGTGT TGTGACATCC
    CTCAAGAACG TCTTCAACAT CCTCATTGTC TACAAGCTCT TCATGTTCAT CTTTGCTGTC
    ATCGCCGTCC AGCTCTTCAA GGGGAAGTTT TTCTACTGCA CTGACAGCTC TAAGGACACA
    GAGAAGGACT GCATAGGGAA CTATGTGGAC CATGAGAAGA ACAAGATGGA GGTGAAGTGC
    CGGGAGTGGA AACGACATGA GTTTCACTAT GACAATATAA TCTGGGCGTT GCTCACCCTG
    TTCACAGTCT CCACCGGCGA GGGTTGGCCC CAGGTGCTGC AGCATTCGGT GGATGTGACG
    GAGGAGGACC GTGGGCCCAG CCGCAGTAAC CGCATGGAGA TGTCCATTTT TTACGTTGTC
    TATTTTGTGG TCTTCCCTTT CTTCTTCGTC AACATCTTTG TGGCGTTCAT CATCATCACC
    TTCCAAGAGC AAGGAGACAA AATGATGGAG GAGTGCAGTC TGGAGAAGAA CGAGAGGGCC
    TGTATTGACT TTGCCATCAG TGCCAAGCCC CTCACACGCT ACATGCCCCA GAACCGGCAC
    ACCTTCCAGT ATCGGGTCTG GCACTTTGTG GTCTCTCCAT CCTTTGAGTA TACCATCATG
    GCCATGATTG CTTTGAACAC TGTGGTGCTG ATGATGAAGT ACTACTCTGC TCCATACACC
    TATGAGCTGG CCCTGAAGTA CCTGAACATC GCTTTCACCA TGGTGTTCTC TTTGGAGTGC
    GTCCTTAAGA TCATTGCTTT TGGCTTCCTG AATTATTTCC GGGATACCTG GAATATCTTT
    GACTTCATCA CCGTCATCGG CAGCATCACA GAGATCATCC TGACAGACAC CAAGCTGGTG
    AACACCAGTA GCTTCAACAT GAGTTTCCTG AAACTCTTCC GGGCGGCTCG GCTCATCAAG
    CTGCTGCGCC AGGGTTACAC CATCCGCATC CTGCTGTGGA CGTTCGTGCA GTCCTTCAAG
    GCTCTCCCCT ATGTCTGCCT CCTAATCGCG ATGCTTTTCT TCATCTATGC CATCATTGGG
    ATGCAGGTTT TCGGCAACAT CAAACTGGAT GAGGAAAGCC ACATTAACCG GCACAACAAC
    TTCCGCAGCT TCCTGGGCTC CCTCATGCTC CTCTTCAGGA GTGCCACCGG TGAGGCATGG
    CAGGAGATCA TGCTCTCCTG CCTGGAGGGC AAGGGCTGCG AGCCGGACAC GACAGCGACG
    TCCGGGCAGA ATGAGAACGA GCGCTGCGGC ACCGACCTGG CCTACGTCTA CTTTGTCTCC
    TTCATCTTCT TCTGCTCATT CCTGATGCTC AACCTGTTCG TGGCTGTCAT CATGGACAAC
    TTTGAGTACC TGACTCGAGA CTCCTCCATC CTGGGACCTC ACCATCTAGA TGAATTTGTC
    CGGATCTGGG CAGAGTATGA CAGAGCAGCG TGTGGCCGAA TCCATTACAC TGAGATGTAT
    GAAATGCTGA CTCTTATGTC ACCACCGCTA GGCCTCGGCA AGAGATGTCC TTCCAAAGTG
    GCCTATAAGA GACTGGTGCT GATGAACATG CCCGTGGCCG AGGACATGAC GGTCCATTTC
    ACCTCCACCC TGATGGCGCT GATACGCACC GCCCTGGACA TCAAAATTGC CAAAGGTGGT
    GCAGATTGGC AGCAGTTAGA CTCTGAGCTT CAAAAGGAGA TCCTGACCAT TTGGCCTCAC
    CTGTCCCAGA AGATGCTTGA CCTGTTGGTA CCCATGCCAA AAACCTCTGA CCTGACTGTT
    GGCAAAATAT ATGCAGCCAT GATGATCATG GATTACTACA AGCAGAGCAA GGCGAAGAAG
    CAGAGGCAGC AGCTGGAGGA GCAGAAAAAT GCGCCCATGT TCCAGCGCAT GGAGCCATCC
    TCTTTGCCAC AGGAAATCAT CTCCAACGCA AAGGCTCTGC CTTACCTCCA GCAGGAGACC
    CTCACAGGGC TGAGCAGCCG GAGCGGGTTC CCCTCGCTGA GCCCGCTCTC ACCACAGGAG
    ATCTTCCAGC TGGCGTGCAT GGATCCGGCC CACGGGCAGT TCCAGGAGCA CCAGTCTCTG
    GTGGTGACGG ACACCAGCTC GATGCGGCGG TCATTTTCCA CCATCCGGGA CAAACGCACC
    AACACCTCCT GGCTGGATGA GTTCTCCATG GAGCGCAGCA GCGACAACAC CTACAAGTCC
    CGCCGGCGCA GCTACCACTC CTCTCTCCAG CTCTCTGCCC GGCGCCTCAA CGCTGACTCA
    GGCCACAGGT CTGAGGGGCA CCGCTCGGGC GGCAGGGAGC GGGGCCGATC CAAAGAGCGC
    AAGCACTTGC TGTCCCCTGA CATCTCCCGC TGCAACTCAG AGGAGAGGAG TCCCCAGGCA
    CACGATGAGT CCCCAGAGCG GCGGCGCGAG TCCCGGTCAC CCAGCGAGGG CAGGTCGCAA
    ACGCCCAACC GGCAGGGAAC GGGCTCCCTG AGCGAAAGCT CCATCCCCTC CATCTCGGAC
    ACCAGCACAC CCCGGCGAGG CCGCCGCCAG CTCCCACCTG TGCCCCCAAA ACCGCGTCCC
    CTCCTCTCCT ACGCCTCCAT GCTGCGGCAC GCTGCCGGAG ACGCCTCACC ACCTCCCGAT
    GAGACCGAAG GGGGGTCCCC GCTCCTCTCC AAGGAGCCCA ACGCCACCGG CCTGACCGAG
    TCTTCCAGCT CCCCGGCGGG GAAGCCGAGC CGGCCGTCCA CCCCGCAGCG TTACATCTCG
    GAGCCCTACC TGGCCCTGCA CGACGACTCG CACGCTTCGG ACTGCGGCGA GGAGGAGACG
    CTCACCTTCG AGGCGGCCGT GGCCACCAGT CTGGGTCGTT CCAACACCAT CGGCTCGGCC
    CCACCGCTGC GACACAGCTG GCAGATGCCC AACGGGCACT ACCGGAGACG GAGGCGAGGG
    GCCGGGCAGG GCGTGATGTG CGGGGCCAGC ATCGATGTGC TCAGCGACAC CGAGGAAGAT
    GACAAGTGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145973.1
    CDS121..7212
    Translation

    Target ORF information:

    RefSeq Version XM_015290487.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAGC GGGAGCGGCG CCGGAGGCAC
    CACATGTCGG TGTGGGAGCA GCGCACCAGC CAGCTGCGCC GGCACATGCA GATGTCCAGC
    CAGGAGGGCA TCAATAAGGA TGAGCCACCC CTCATCAACC CACATGCCAG CATCTTCCGC
    CGCAAGAAGC CGGGGGACGG TGTTGCCTTG GAGAAGTGCG ATGAAGAGCA AGGCGGCAAG
    GGTGAGCGGC CGCCAGCTGA GGGTCCCGAG CAGCCGGCCC CAGGGACCAA CCCAGGCGGT
    GGGGAGGACA GGAAGAGCCC ATCGCCCCGT GCCAGGAGGG ACAAGGAGAT GTGGCAACAC
    AAAGCGTGCC ACGGGAACTG CGAGCCGGGT GAGGAAGGGG CAGGAGGTGG CATCGAGGAG
    AGGGCGCGGA TGAGACAGAG CCAGCGGCGC AGCCGGCATC GGAAAGCCAG GATGGAGGGG
    AAGGAGACGA CCGGAGCCCT GGAGAGCCGT GCGGGCAGCC AGGAGATGGG GCTGGAGGAG
    CCCTGCCCCG CAGAGGGGAT GCAGGATGGG GAGCGGCGTG GGGATTCTGC TGCCGCGCTG
    GACCTGATTC AGGGCGAGCT GGAGGCGAGC GGGGACCCCA CCAGGACTAA TGGGGTTCCC
    GCCGAGGAGG GAAGCCTCCC AGCATCAGCC CCTGAGCCTT CCCGGGGGAT GGAGGGCAGC
    CTGGGTGAGC AGGACTGCAG CAGCCCAGAC ACCAGTGAGC AGGCCCTGCT GGGGGGGGCG
    GCACTGGGGG CCAGCCGCAC CGTCAGCCAC AGCGAGCCTG ACCTGTCCTC CGTCACCGCC
    AACACTGAGA AGGCCACCGA GAGCACCACC ATCATGATTG ATGTCCAAGA CTCCACTGTG
    GTACAGATTA GCAACAAGAC TGATGGAGAA GCCAGCCCCT TGAAGGAGGC AGAGAGCAAA
    GAGGATGAGG AAGAGATGGA GAAGAAGAAG CGCAAGAAGG AGAAGAGCGA GACGGGCAAA
    GCGATGGTGC CACACAGCTC CATGTTCATC TTCAGCACCA CAAACCCGGT GCGCAGAGCC
    TGCCACTACA TTGTGAACCT GCGCTACTTT GAGATGTGCA TCCTCCTGGT CATCGCCGCC
    AGCAGCATCG CCCTCGCGGC CGAGGATCCC GTCCTCACCA ACTCCGACCG TAACAAAGTG
    CTGAGGTATT TTGATTACGT TTTCACCGGT GTCTTCACCT TCGAGATGGT TATCAAGATG
    ATCGACCAGG GCTTGATCCT GCAGGATGGC TCCTACTTCC GAGACCTCTG GAACATCCTG
    GATTTCATCG TGGTGGTGGG AGCTCTGGTG GCCTTTGCCT TGGCGAATGC TTTGGGGACC
    AACAAGGGTC GGGATATCAA GACTATCAAG TCTCTCCGTG TGCTGCGGGT CTTGAGGCCC
    CTGAAAACCA TCAAGCGGCT GCCGAAGCTC AAGGCTGTCT TTGACTGTGT TGTGACATCC
    CTCAAGAACG TCTTCAACAT CCTCATTGTC TACAAGCTCT TCATGTTCAT CTTTGCTGTC
    ATCGCCGTCC AGCTCTTCAA GGGGAAGTTT TTCTACTGCA CTGACAGCTC TAAGGACACA
    GAGAAGGACT GCATAGGGAA CTATGTGGAC CATGAGAAGA ACAAGATGGA GGTGAAGTGC
    CGGGAGTGGA AACGACATGA GTTTCACTAT GACAATATAA TCTGGGCGTT GCTCACCCTG
    TTCACAGTCT CCACCGGCGA GGGTTGGCCC CAGGTGCTGC AGCATTCGGT GGATGTGACG
    GAGGAGGACC GTGGGCCCAG CCGCAGTAAC CGCATGGAGA TGTCCATTTT TTACGTTGTC
    TATTTTGTGG TCTTCCCTTT CTTCTTCGTC AACATCTTTG TGGCGTTCAT CATCATCACC
    TTCCAAGAGC AAGGAGACAA AATGATGGAG GAGTGCAGTC TGGAGAAGAA CGAGAGGGCC
    TGTATTGACT TTGCCATCAG TGCCAAGCCC CTCACACGCT ACATGCCCCA GAACCGGCAC
    ACCTTCCAGT ATCGGGTCTG GCACTTTGTG GTCTCTCCAT CCTTTGAGTA TACCATCATG
    GCCATGATTG CTTTGAACAC TGTGGTGCTG ATGATGAAGT ACTACTCTGC TCCATACACC
    TATGAGCTGG CCCTGAAGTA CCTGAACATC GCTTTCACCA TGGTGTTCTC TTTGGAGTGC
    GTCCTTAAGA TCATTGCTTT TGGCTTCCTG AATTATTTCC GGGATACCTG GAATATCTTT
    GACTTCATCA CCGTCATCGG CAGCATCACA GAGATCATCC TGACAGACAC CAAGCTGGTG
    AACACCAGTA GCTTCAACAT GAGTTTCCTG AAACTCTTCC GGGCGGCTCG GCTCATCAAG
    CTGCTGCGCC AGGGTTACAC CATCCGCATC CTGCTGTGGA CGTTCGTGCA GTCCTTCAAG
    GCTCTCCCCT ATGTCTGCCT CCTAATCGCG ATGCTTTTCT TCATCTATGC CATCATTGGG
    ATGCAGGTTT TCGGCAACAT CAAACTGGAT GAGGAAAGCC ACATTAACCG GCACAACAAC
    TTCCGCAGCT TCCTGGGCTC CCTCATGCTC CTCTTCAGGA GTGCCACCGG TGAGGCATGG
    CAGGAGATCA TGCTCTCCTG CCTGGAGGGC AAGGGCTGCG AGCCGGACAC GACAGCGACG
    TCCGGGCAGA ATGAGAACGA GCGCTGCGGC ACCGACCTGG CCTACGTCTA CTTTGTCTCC
    TTCATCTTCT TCTGCTCATT CCTGATGCTC AACCTGTTCG TGGCTGTCAT CATGGACAAC
    TTTGAGTACC TGACTCGAGA CTCCTCCATC CTGGGACCTC ACCATCTAGA TGAATTTGTC
    CGGATCTGGG CAGAGTATGA CAGAGCAGCG TGTGGCCGAA TCCATTACAC TGAGATGTAT
    GAAATGCTGA CTCTTATGTC ACCACCGCTA GGCCTCGGCA AGAGATGTCC TTCCAAAGTG
    GCCTATAAGA GACTGGTGCT GATGAACATG CCCGTGGCCG AGGACATGAC GGTCCATTTC
    ACCTCCACCC TGATGGCGCT GATACGCACC GCCCTGGACA TCAAAATTGC CAAAGGTGGT
    GCAGATTGGC AGCAGTTAGA CTCTGAGCTT CAAAAGGAGA TCCTGACCAT TTGGCCTCAC
    CTGTCCCAGA AGATGCTTGA CCTGTTGGTA CCCATGCCAA AAACCTCTGA CCTGACTGTT
    GGCAAAATAT ATGCAGCCAT GATGATCATG GATTACTACA AGCAGAGCAA GGCGAAGAAG
    CAGAGGCAGC AGCTGGAGGA GCAGAAAAAT GCGCCCATGT TCCAGCGCAT GGAGCCATCC
    TCTTTGCCAC AGGAAATCAT CTCCAACGCA AAGGCTCTGC CTTACCTCCA GCAGGAGACC
    CTCACAGGGC TGAGCAGCCG GAGCGGGTTC CCCTCGCTGA GCCCGCTCTC ACCACAGGAG
    ATCTTCCAGC TGGCGTGCAT GGATCCGGCC CACGGGCAGT TCCAGGAGCA CCAGTCTCTG
    GTGGTGACGG ACACCAGCTC GATGCGGCGG TCATTTTCCA CCATCCGGGA CAAACGCACC
    AACACCTCCT GGCTGGATGA GTTCTCCATG GAGCGCAGCA GCGACAACAC CTACAAGTCC
    CGCCGGCGCA GCTACCACTC CTCTCTCCAG CTCTCTGCCC GGCGCCTCAA CGCTGACTCA
    GGCCACAGGT CTGAGGGGCA CCGCTCGGGC GGCAGGGAGC GGGGCCGATC CAAAGAGCGC
    AAGCACTTGC TGTCCCCTGA CATCTCCCGC TGCAACTCAG AGGAGAGGAG TCCCCAGGCA
    CACGATGAGT CCCCAGAGCG GCGGCGCGAG TCCCGGTCAC CCAGCGAGGG CAGGTCGCAA
    ACGCCCAACC GGCAGGGAAC GGGCTCCCTG AGCGAAAGCT CCATCCCCTC CATCTCGGAC
    ACCAGCACAC CCCGGCGAGG CCGCCGCCAG CTCCCACCTG TGCCCCCAAA ACCGCGTCCC
    CTCCTCTCCT ACGCCTCCAT GCTGCGGCAC GCTGCCGGAG ACGCCTCACC ACCTCCCGAT
    GAGACCGAAG GGGGGTCCCC GCTCCTCTCC AAGGAGCCCA ACGCCACCGG CCTGACCGAG
    TCTTCCAGCT CCCCGGCGGG GAAGCCGAGC CGGCCGTCCA CCCCGCAGCG TTACATCTCG
    GAGCCCTACC TGGCCCTGCA CGACGACTCG CACGCTTCGG ACTGCGGCGA GGAGGAGACG
    CTCACCTTCG AGGCGGCCGT GGCCACCAGT CTGGGTCGTT CCAACACCAT CGGCTCGGCC
    CCACCGCTGC GACACAGCTG GCAGATGCCC AACGGGCACT ACCGGAGACG GAGGCGAGGG
    GCCGGGCAGG GCGTGATGTG CGGGGCCAGC ATCGATGTGC TCAGCGACAC CGAGGAAGAT
    GACAAGTGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39022
    Clone ID Related Accession (Same CDS sequence) XM_015290488.1
    Accession Version XM_015290488.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAGC GGGAGCGGCG CCGGAGGCAC
    CACATGTCGG TGTGGGAGCA GCGCACCAGC CAGCTGCGCC GGCACATGCA GATGTCCAGC
    CAGGAGGGCA TCAATAAGGA TGAGCCACCC CTCATCAACC CACATGCCAG CATCTTCCGC
    CGCAAGAAGC CGGGGGACGG TGTTGCCTTG GAGAAGTGCG ATGAAGAGCA AGGCGGCAAG
    GGTGAGCGGC CGCCAGCTGA GGGTCCCGAG CAGCCGGCCC CAGGGACCAA CCCAGGCGGT
    GGGGAGGACA GGAAGAGCCC ATCGCCCCGT GCCAGGAGGG ACAAGGAGAT GTGGCAACAC
    AAAGCGTGCC ACGGGAACTG CGAGCCGGGT GAGGAAGGGG CAGGAGGTGG CATCGAGGAG
    AGGGCGCGGA TGAGACAGAG CCAGCGGCGC AGCCGGCATC GGAAAGCCAG GATGGAGGGG
    AAGGAGACGA CCGGAGCCCT GGAGAGCCGT GCGGGCAGCC AGGAGATGGG GCTGGAGGAG
    CCCTGCCCCG CAGAGGGGAT GCAGGATGGG GAGCGGCGTG GGGATTCTGC TGCCGCGCTG
    GACCTGATTC AGGGCGAGCT GGAGGCGAGC GGGGACCCCA CCAGGACTAA TGGGGTTCCC
    GCCGAGGAGG GAAGCCTCCC AGCATCAGCC CCTGAGCCTT CCCGGGGGAT GGAGGGCAGC
    CTGGGTGAGC AGGACTGCAG CAGCCCAGAC ACCAGTGAGC AGGCCCTGCT GGGGGGGGCG
    GCACTGGGGG CCAGCCGCAC CGTCAGCCAC AGCGAGCCTG ACCTGTCCTC CGTCACCGCC
    AACACTGAGA AGGCCACCGA GAGCACCACC ATCATGATTG ATGTCCAAGA CTCCACTGTG
    GTACAGATTA GCAACAAGAC TGATGGAGAA GCCAGCCCCT TGAAGGAGGC AGAGAGCAAA
    GAGGATGAGG AAGAGATGGA GAAGAAGAAG CGCAAGAAGG AGAAGAGCGA GACGGGCAAA
    GCGATGGTGC CACACAGCTC CATGTTCATC TTCAGCACCA CAAACCCGGT GCGCAGAGCC
    TGCCACTACA TTGTGAACCT GCGCTACTTT GAGATGTGCA TCCTCCTGGT CATCGCCGCC
    AGCAGCATCG CCCTCGCGGC CGAGGATCCC GTCCTCACCA ACTCCGACCG TAACAAAGTG
    CTGAGGTATT TTGATTACGT TTTCACCGGT GTCTTCACCT TCGAGATGGT TATCAAGATG
    ATCGACCAGG GCTTGATCCT GCAGGATGGC TCCTACTTCC GAGACCTCTG GAACATCCTG
    GATTTCATCG TGGTGGTGGG AGCTCTGGTG GCCTTTGCCT TGGCGAATGC TTTGGGGACC
    AACAAGGGTC GGGATATCAA GACTATCAAG TCTCTCCGTG TGCTGCGGGT CTTGAGGCCC
    CTGAAAACCA TCAAGCGGCT GCCGAAGCTC AAGGCTGTCT TTGACTGTGT TGTGACATCC
    CTCAAGAACG TCTTCAACAT CCTCATTGTC TACAAGCTCT TCATGTTCAT CTTTGCTGTC
    ATCGCCGTCC AGCTCTTCAA GGGGAAGTTT TTCTACTGCA CTGACAGCTC TAAGGACACA
    GAGAAGGACT GCATAGGGAA CTATGTGGAC CATGAGAAGA ACAAGATGGA GGTGAAGTGC
    CGGGAGTGGA AACGACATGA GTTTCACTAT GACAATATAA TCTGGGCGTT GCTCACCCTG
    TTCACAGTCT CCACCGGCGA GGGTTGGCCC CAGGTGCTGC AGCATTCGGT GGATGTGACG
    GAGGAGGACC GTGGGCCCAG CCGCAGTAAC CGCATGGAGA TGTCCATTTT TTACGTTGTC
    TATTTTGTGG TCTTCCCTTT CTTCTTCGTC AACATCTTTG TGGCGTTCAT CATCATCACC
    TTCCAAGAGC AAGGAGACAA AATGATGGAG GAGTGCAGTC TGGAGAAGAA CGAGAGGGCC
    TGTATTGACT TTGCCATCAG TGCCAAGCCC CTCACACGCT ACATGCCCCA GAACCGGCAC
    ACCTTCCAGT ATCGGGTCTG GCACTTTGTG GTCTCTCCAT CCTTTGAGTA TACCATCATG
    GCCATGATTG CTTTGAACAC TGTGGTGCTG ATGATGAAGT ACTACTCTGC TCCATACACC
    TATGAGCTGG CCCTGAAGTA CCTGAACATC GCTTTCACCA TGGTGTTCTC TTTGGAGTGC
    GTCCTTAAGA TCATTGCTTT TGGCTTCCTG AATTATTTCC GGGATACCTG GAATATCTTT
    GACTTCATCA CCGTCATCGG CAGCATCACA GAGATCATCC TGACAGACAC CAAGCTGGTG
    AACACCAGTA GCTTCAACAT GAGTTTCCTG AAACTCTTCC GGGCGGCTCG GCTCATCAAG
    CTGCTGCGCC AGGGTTACAC CATCCGCATC CTGCTGTGGA CGTTCGTGCA GTCCTTCAAG
    GCTCTCCCCT ATGTCTGCCT CCTAATCGCG ATGCTTTTCT TCATCTATGC CATCATTGGG
    ATGCAGGTTT TCGGCAACAT CAAACTGGAT GAGGAAAGCC ACATTAACCG GCACAACAAC
    TTCCGCAGCT TCCTGGGCTC CCTCATGCTC CTCTTCAGGA GTGCCACCGG TGAGGCATGG
    CAGGAGATCA TGCTCTCCTG CCTGGAGGGC AAGGGCTGCG AGCCGGACAC GACAGCGACG
    TCCGGGCAGA ATGAGAACGA GCGCTGCGGC ACCGACCTGG CCTACGTCTA CTTTGTCTCC
    TTCATCTTCT TCTGCTCATT CCTGATGCTC AACCTGTTCG TGGCTGTCAT CATGGACAAC
    TTTGAGTACC TGACTCGAGA CTCCTCCATC CTGGGACCTC ACCATCTAGA TGAATTTGTC
    CGGATCTGGG CAGAGTATGA CAGAGCAGCG TGCGGCAGGA TCTCGTATAA GGATATGTAC
    AAATTAGTAC GGGTGATCTC GCCTCCTCTG GGCCTAGGAA AGAACTGCCC CTACAGGGTG
    GCGTGCAAGA GACTGGTGCT GATGAACATG CCCGTGGCCG AGGACATGAC GGTCCATTTC
    ACCTCCACCC TGATGGCGCT GATACGCACC GCCCTGGACA TCAAAATTGC CAAAGGTGGT
    GCAGATTGGC AGCAGTTAGA CTCTGAGCTT CAAAAGGAGA TCCTGACCAT TTGGCCTCAC
    CTGTCCCAGA AGATGCTTGA CCTGTTGGTA CCCATGCCAA AAACCTCTGA CCTGACTGTT
    GGCAAAATAT ATGCAGCCAT GATGATCATG GATTACTACA AGCAGAGCAA GGCGAAGAAG
    CAGAGGCAGC AGCTGGAGGA GCAGAAAAAT GCGCCCATGT TCCAGCGCAT GGAGCCATCC
    TCTTTGCCAC AGGAAATCAT CTCCAACGCA AAGGCTCTGC CTTACCTCCA GCAGGAGACC
    CTCACAGGGC TGAGCAGCCG GAGCGGGTTC CCCTCGCTGA GCCCGCTCTC ACCACAGGAG
    ATCTTCCAGC TGGCGTGCAT GGATCCGGCC CACGGGCAGT TCCAGGAGCA CCAGTCTCTG
    GTGGTGACGG ACACCAGCTC GATGCGGCGG TCATTTTCCA CCATCCGGGA CAAACGCACC
    AACACCTCCT GGCTGGATGA GTTCTCCATG GAGCGCAGCA GCGACAACAC CTACAAGTCC
    CGCCGGCGCA GCTACCACTC CTCTCTCCAG CTCTCTGCCC GGCGCCTCAA CGCTGACTCA
    GGCCACAGGT CTGAGGGGCA CCGCTCGGGC GGCAGGGAGC GGGGCCGATC CAAAGAGCGC
    AAGCACTTGC TGTCCCCTGA CATCTCCCGC TGCAACTCAG AGGAGAGGAG TCCCCAGGCA
    CACGATGAGT CCCCAGAGCG GCGGCGCGAG TCCCGGTCAC CCAGCGAGGG CAGGTCGCAA
    ACGCCCAACC GGCAGGGAAC GGGCTCCCTG AGCGAAAGCT CCATCCCCTC CATCTCGGAC
    ACCAGCACAC CCCGGCGAGG CCGCCGCCAG CTCCCACCTG TGCCCCCAAA ACCGCGTCCC
    CTCCTCTCCT ACGCCTCCAT GCTGCGGCAC GCTGCCGGAG ACGCCTCACC ACCTCCCGAT
    GAGACCGAAG GGGGGTCCCC GCTCCTCTCC AAGGAGCCCA ACGCCACCGG CCTGACCGAG
    TCTTCCAGCT CCCCGGCGGG GAAGCCGAGC CGGCCGTCCA CCCCGCAGCG TTACATCTCG
    GAGCCCTACC TGGCCCTGCA CGACGACTCG CACGCTTCGG ACTGCGGCGA GGAGGAGACG
    CTCACCTTCG AGGCGGCCGT GGCCACCAGT CTGGGTCGTT CCAACACCAT CGGCTCGGCC
    CCACCGCTGC GACACAGCTG GCAGATGCCC AACGGGCACT ACCGGAGACG GAGGCGAGGG
    GCCGGGCAGG GCGTGATGTG CGGGGCCAGC ATCGATGTGC TCAGCGACAC CGAGGAAGAT
    GACAAGTGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145974.1
    CDS122..7213
    Translation

    Target ORF information:

    RefSeq Version XM_015290488.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290488.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGCTCGGAC TCGGCACGTG CTCCGTCAGC GCCCCGGGCA GGGCGGCTGA GCCGGCCGCA 
    TCCCCCCACT ACGCACTGGC TCTGCCCGAG GAGGAGATGT GGCGCGAGCA CCGACTCGCC
    CTGATGCACA GCACCCAGTC CTGCAACCTG CTGGAGCCCC AGAGCCTGGC TGAGGTGCTG
    GTCTCCCGTG CGACCAGCTT CGATGCCCTC TACCAACCCC GAGATGCTGA CGGTGACAGC
    GGCAGCGCCT TGGACCTGGG GCTTGGAACC TACGTCCCCA TCAGCCCCGA TGTCATCAAG
    CGCCGGCGGG GAGGGCTGAT TGAGCAGAGG GACATCATCA AGGCACACGA GGCACACAAG
    ATGCAGAGCA CGCCGCAGGC AAGGAGGAAG GAGTGGGAGA TGGCTCGCTT CGGGGAGGCG
    GTGGCTGGCA GGCTGGGCTC CGGGGATGGG GGCTCCGAGC AGAACCGCAG CCGGCAAGGA
    GCCGCTGCTC CGGCGGGAGG GTCCCCGGGA GCCTTCAAGC AAACCAAAGC GCAGCGAGCC
    CGGACTATGG CTCTCTACAA CCCCATCCCC GTCCGGCAGA ACTGCTTCAC CGTCAACAGA
    TCGTTGTTCC TCTTTGGGGA AGATAACATA GTCAGGAAAT ACGCCAAGAA GCTCATCGAC
    TGGCCTCCCT TCGAGTATAT GATCCTGGCA ACCATAATTG CCAACTGCAT CGTGCTGGCC
    CTGGAGCAGC ACCTCCCAGA GGACGACAAG ACACCCATGT CACGGAGACT GGAGAAGACG
    GAGCCGTACT TTATTGGGAT TTTCTGCTTT GAGGCTGGGA TTAAGATCGT GGCTCTGGGC
    TTCGTTTTCC ACAAGGGCTC ATACCTACGC AATGGCTGGA ACGTCATGGA CTTCATCGTG
    GTCCTCAGTG GCATCCTTGC CACCGCTGGA ACACACTTCA ACACGCACGT AGACCTGCGC
    ACCCTGCGGG CCGTGCGTGT GCTCAGGCCT CTGAAGCTCG TCTCGGGCAT TCCCAGCCTT
    CAGATTGTGC TGAAATCCAT CATGAAGGCC ATGGTACCTC TCCTCCAGAT TGGCTTGCTG
    CTCTTTTTCG CTATTCTCAT GTTTGCCATC ATCGGCCTGG AGTTTTACAG TGGGAAACTG
    CACCGAGCCT GCTACACAAA CAATTCAGGT GAGCTGGAGG AGCTGGACCC TCCCCACCCC
    TGCGGGGTGC AGGGCTGTCC GCCGGGCTAT GAGTGCCGGG AGTGGATTGG TCCCAATGAT
    GGGATCACGC AGTTCGACAA CATCCTCTTT GCTGTGCTGA CCGTCTTCCA GTGCATCACC
    ATGGAAGGGT GGACCACAGT TCTGTACAAT ACCAATGATG CCTTAGGAGC CACCTGGAAC
    TGGCTATACT TCATCCCCCT CATCATCATT GGATCCTTCT TCGTCCTCAA CCTTGTCCTG
    GGAGTGCTGT CAGGGGAGTT TGCCAAAGAA CGTGAGCGCG TGGAGAACCG CCGGGCCTTC
    ATGAAGCTGC GGCGACAGCA GCAGATCGAG AGGGAGCTCA ACGGCTACCG AGCCTGGATA
    GACAAAGCGG AGGAAGTCAT GCTGGCTGAA GAGAACAAAA ACGCTGGGAC CTCAGCCTTA
    GAAGTGCTCA GGAGAGCCAC CATCAAGAGG AACCGGACGG ATGCCATGAA CCGTGACTCC
    AGCGATGAGC ACTGCGTCGA TATCTCCTCC GTGGGCGAGC GGAGGCTGGC ACAGAGGAGG
    CCTCTTCATC CCACCTTGGG TAACCCCTTG AGTCGCTCCG GTCTCAAAGG AGTAGATGGA
    GCCTCCTATT TACGGCACAA GGAGCGGCTC CTGCGCATTT CTGTTCGTCA CATGGTGAAA
    TCCCAGGTCT TCTACTGGAT TGTCTTGAGC CTGGTGGCTC TTAACACTGC CTGTGTGGCC
    ATCGTCCATC ACAACCAGCC AGCCTGGCTC ACCCACTTCC TCTACTATGC AGAGTTCCTG
    TTTCTTGGGC TCTTCCTCCT GGAGATGTCC TTGAAGATGT ATGGGATGGG GCCACGCCTG
    TACTTCCATT CTTCATTCAA CTGTTTCGAC TGTGGGGTCA CAGTGGGAAG CATCTTTGAA
    GTGGTCTGGG CCATCTTCCG ACCAGGGACC TCTTTTGGGA TCAGTGTCCT ACGAGCCCTT
    CGTCTCCTGC GAATATTTAA AATAACCAAG TACTGGGCAT CCTTGAGGAA TTTGGTGGTC
    TCGCTGATGA GCTCTATGAA ATCTATTATC AGCCTGCTCT TCTTGCTCTT TCTCTTCATT
    GTAGTCTTTG CCCTGCTGGG AATGCAGCTG TTTGGAGGCG GGTTTAATTT CATTGATGGG
    ACACCATCGG CCAACTTTGA CACTTTCCCT GCAGCCATCA TGACGGTGTT CCAGATCTTG
    ACGGGTGAGG ACTGGAATGA GGTGATGTAC AATGGCATCC GCTCCCAGGG AGGTGTTCGC
    TCGGGCATGT GGTCCTCCAT CTATTTCATC GTCCTCACCT TGTTTGGGAA CTATACTCTG
    CTGAATGTCT TCTTGGCCAT TGCAGTGGAC AACCTGGCTA ATGCTCAGGA GCTCACCAAG
    GACGAGCAGG AGGAAGAGGA GGCCTTTAAC CAGAAGCATG CCCTTCAGAA AGCCAAGGAA
    GTCAGCCCCA TGTCTGCCCC CAACATGCCT GCTATTGAGC GGGAGCGGCG CCGGAGGCAC
    CACATGTCGG TGTGGGAGCA GCGCACCAGC CAGCTGCGCC GGCACATGCA GATGTCCAGC
    CAGGAGGGCA TCAATAAGGA TGAGCCACCC CTCATCAACC CACATGCCAG CATCTTCCGC
    CGCAAGAAGC CGGGGGACGG TGTTGCCTTG GAGAAGTGCG ATGAAGAGCA AGGCGGCAAG
    GGTGAGCGGC CGCCAGCTGA GGGTCCCGAG CAGCCGGCCC CAGGGACCAA CCCAGGCGGT
    GGGGAGGACA GGAAGAGCCC ATCGCCCCGT GCCAGGAGGG ACAAGGAGAT GTGGCAACAC
    AAAGCGTGCC ACGGGAACTG CGAGCCGGGT GAGGAAGGGG CAGGAGGTGG CATCGAGGAG
    AGGGCGCGGA TGAGACAGAG CCAGCGGCGC AGCCGGCATC GGAAAGCCAG GATGGAGGGG
    AAGGAGACGA CCGGAGCCCT GGAGAGCCGT GCGGGCAGCC AGGAGATGGG GCTGGAGGAG
    CCCTGCCCCG CAGAGGGGAT GCAGGATGGG GAGCGGCGTG GGGATTCTGC TGCCGCGCTG
    GACCTGATTC AGGGCGAGCT GGAGGCGAGC GGGGACCCCA CCAGGACTAA TGGGGTTCCC
    GCCGAGGAGG GAAGCCTCCC AGCATCAGCC CCTGAGCCTT CCCGGGGGAT GGAGGGCAGC
    CTGGGTGAGC AGGACTGCAG CAGCCCAGAC ACCAGTGAGC AGGCCCTGCT GGGGGGGGCG
    GCACTGGGGG CCAGCCGCAC CGTCAGCCAC AGCGAGCCTG ACCTGTCCTC CGTCACCGCC
    AACACTGAGA AGGCCACCGA GAGCACCACC ATCATGATTG ATGTCCAAGA CTCCACTGTG
    GTACAGATTA GCAACAAGAC TGATGGAGAA GCCAGCCCCT TGAAGGAGGC AGAGAGCAAA
    GAGGATGAGG AAGAGATGGA GAAGAAGAAG CGCAAGAAGG AGAAGAGCGA GACGGGCAAA
    GCGATGGTGC CACACAGCTC CATGTTCATC TTCAGCACCA CAAACCCGGT GCGCAGAGCC
    TGCCACTACA TTGTGAACCT GCGCTACTTT GAGATGTGCA TCCTCCTGGT CATCGCCGCC
    AGCAGCATCG CCCTCGCGGC CGAGGATCCC GTCCTCACCA ACTCCGACCG TAACAAAGTG
    CTGAGGTATT TTGATTACGT TTTCACCGGT GTCTTCACCT TCGAGATGGT TATCAAGATG
    ATCGACCAGG GCTTGATCCT GCAGGATGGC TCCTACTTCC GAGACCTCTG GAACATCCTG
    GATTTCATCG TGGTGGTGGG AGCTCTGGTG GCCTTTGCCT TGGCGAATGC TTTGGGGACC
    AACAAGGGTC GGGATATCAA GACTATCAAG TCTCTCCGTG TGCTGCGGGT CTTGAGGCCC
    CTGAAAACCA TCAAGCGGCT GCCGAAGCTC AAGGCTGTCT TTGACTGTGT TGTGACATCC
    CTCAAGAACG TCTTCAACAT CCTCATTGTC TACAAGCTCT TCATGTTCAT CTTTGCTGTC
    ATCGCCGTCC AGCTCTTCAA GGGGAAGTTT TTCTACTGCA CTGACAGCTC TAAGGACACA
    GAGAAGGACT GCATAGGGAA CTATGTGGAC CATGAGAAGA ACAAGATGGA GGTGAAGTGC
    CGGGAGTGGA AACGACATGA GTTTCACTAT GACAATATAA TCTGGGCGTT GCTCACCCTG
    TTCACAGTCT CCACCGGCGA GGGTTGGCCC CAGGTGCTGC AGCATTCGGT GGATGTGACG
    GAGGAGGACC GTGGGCCCAG CCGCAGTAAC CGCATGGAGA TGTCCATTTT TTACGTTGTC
    TATTTTGTGG TCTTCCCTTT CTTCTTCGTC AACATCTTTG TGGCGTTCAT CATCATCACC
    TTCCAAGAGC AAGGAGACAA AATGATGGAG GAGTGCAGTC TGGAGAAGAA CGAGAGGGCC
    TGTATTGACT TTGCCATCAG TGCCAAGCCC CTCACACGCT ACATGCCCCA GAACCGGCAC
    ACCTTCCAGT ATCGGGTCTG GCACTTTGTG GTCTCTCCAT CCTTTGAGTA TACCATCATG
    GCCATGATTG CTTTGAACAC TGTGGTGCTG ATGATGAAGT ACTACTCTGC TCCATACACC
    TATGAGCTGG CCCTGAAGTA CCTGAACATC GCTTTCACCA TGGTGTTCTC TTTGGAGTGC
    GTCCTTAAGA TCATTGCTTT TGGCTTCCTG AATTATTTCC GGGATACCTG GAATATCTTT
    GACTTCATCA CCGTCATCGG CAGCATCACA GAGATCATCC TGACAGACAC CAAGCTGGTG
    AACACCAGTA GCTTCAACAT GAGTTTCCTG AAACTCTTCC GGGCGGCTCG GCTCATCAAG
    CTGCTGCGCC AGGGTTACAC CATCCGCATC CTGCTGTGGA CGTTCGTGCA GTCCTTCAAG
    GCTCTCCCCT ATGTCTGCCT CCTAATCGCG ATGCTTTTCT TCATCTATGC CATCATTGGG
    ATGCAGGTTT TCGGCAACAT CAAACTGGAT GAGGAAAGCC ACATTAACCG GCACAACAAC
    TTCCGCAGCT TCCTGGGCTC CCTCATGCTC CTCTTCAGGA GTGCCACCGG TGAGGCATGG
    CAGGAGATCA TGCTCTCCTG CCTGGAGGGC AAGGGCTGCG AGCCGGACAC GACAGCGACG
    TCCGGGCAGA ATGAGAACGA GCGCTGCGGC ACCGACCTGG CCTACGTCTA CTTTGTCTCC
    TTCATCTTCT TCTGCTCATT CCTGATGCTC AACCTGTTCG TGGCTGTCAT CATGGACAAC
    TTTGAGTACC TGACTCGAGA CTCCTCCATC CTGGGACCTC ACCATCTAGA TGAATTTGTC
    CGGATCTGGG CAGAGTATGA CAGAGCAGCG TGCGGCAGGA TCTCGTATAA GGATATGTAC
    AAATTAGTAC GGGTGATCTC GCCTCCTCTG GGCCTAGGAA AGAACTGCCC CTACAGGGTG
    GCGTGCAAGA GACTGGTGCT GATGAACATG CCCGTGGCCG AGGACATGAC GGTCCATTTC
    ACCTCCACCC TGATGGCGCT GATACGCACC GCCCTGGACA TCAAAATTGC CAAAGGTGGT
    GCAGATTGGC AGCAGTTAGA CTCTGAGCTT CAAAAGGAGA TCCTGACCAT TTGGCCTCAC
    CTGTCCCAGA AGATGCTTGA CCTGTTGGTA CCCATGCCAA AAACCTCTGA CCTGACTGTT
    GGCAAAATAT ATGCAGCCAT GATGATCATG GATTACTACA AGCAGAGCAA GGCGAAGAAG
    CAGAGGCAGC AGCTGGAGGA GCAGAAAAAT GCGCCCATGT TCCAGCGCAT GGAGCCATCC
    TCTTTGCCAC AGGAAATCAT CTCCAACGCA AAGGCTCTGC CTTACCTCCA GCAGGAGACC
    CTCACAGGGC TGAGCAGCCG GAGCGGGTTC CCCTCGCTGA GCCCGCTCTC ACCACAGGAG
    ATCTTCCAGC TGGCGTGCAT GGATCCGGCC CACGGGCAGT TCCAGGAGCA CCAGTCTCTG
    GTGGTGACGG ACACCAGCTC GATGCGGCGG TCATTTTCCA CCATCCGGGA CAAACGCACC
    AACACCTCCT GGCTGGATGA GTTCTCCATG GAGCGCAGCA GCGACAACAC CTACAAGTCC
    CGCCGGCGCA GCTACCACTC CTCTCTCCAG CTCTCTGCCC GGCGCCTCAA CGCTGACTCA
    GGCCACAGGT CTGAGGGGCA CCGCTCGGGC GGCAGGGAGC GGGGCCGATC CAAAGAGCGC
    AAGCACTTGC TGTCCCCTGA CATCTCCCGC TGCAACTCAG AGGAGAGGAG TCCCCAGGCA
    CACGATGAGT CCCCAGAGCG GCGGCGCGAG TCCCGGTCAC CCAGCGAGGG CAGGTCGCAA
    ACGCCCAACC GGCAGGGAAC GGGCTCCCTG AGCGAAAGCT CCATCCCCTC CATCTCGGAC
    ACCAGCACAC CCCGGCGAGG CCGCCGCCAG CTCCCACCTG TGCCCCCAAA ACCGCGTCCC
    CTCCTCTCCT ACGCCTCCAT GCTGCGGCAC GCTGCCGGAG ACGCCTCACC ACCTCCCGAT
    GAGACCGAAG GGGGGTCCCC GCTCCTCTCC AAGGAGCCCA ACGCCACCGG CCTGACCGAG
    TCTTCCAGCT CCCCGGCGGG GAAGCCGAGC CGGCCGTCCA CCCCGCAGCG TTACATCTCG
    GAGCCCTACC TGGCCCTGCA CGACGACTCG CACGCTTCGG ACTGCGGCGA GGAGGAGACG
    CTCACCTTCG AGGCGGCCGT GGCCACCAGT CTGGGTCGTT CCAACACCAT CGGCTCGGCC
    CCACCGCTGC GACACAGCTG GCAGATGCCC AACGGGCACT ACCGGAGACG GAGGCGAGGG
    GCCGGGCAGG GCGTGATGTG CGGGGCCAGC ATCGATGTGC TCAGCGACAC CGAGGAAGAT
    GACAAGTGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.