CACNA1E cDNA ORF clone, Bos taurus(cattle)

The following CACNA1E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa45603 XM_010813517.2
Latest version!
Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 22-24 $1161.30
$1659.00
OBa93040 XM_015475250.1
Latest version!
Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa216174 NM_001191123.2
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa238960 NM_001191123.3
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa223446 XM_024976061.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa223447 XM_015475247.2
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa93037 XM_015475247.1
Latest version!
Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa93038 XM_015475248.1
Latest version!
Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa93039 XM_015475249.1
Latest version!
Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa01606 NM_001191123.1
Latest version!
Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa45603
    Clone ID Related Accession (Same CDS sequence) XM_010813517.2
    Accession Version XM_010813517.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG
    CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG
    GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC
    AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG
    GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA
    GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG
    CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC
    AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC
    AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG
    TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT
    GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA
    TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA
    GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG
    GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC
    GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC
    AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC
    CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC
    TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG
    GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC
    ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC
    ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC
    AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC
    TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC
    CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT
    CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG
    GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC
    TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC
    TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC
    ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA
    AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC
    ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG
    CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG
    ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT
    GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC
    CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC
    TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA
    TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG
    TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC
    ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC
    CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT
    CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC
    CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG
    ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC
    TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT
    CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG
    AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT
    GAGCCCGACA CCACTGCGCC GTCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG
    GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC
    GTGGCTGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT
    CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC
    ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCGCCGCT AGGCCTCGGC
    AAGAGATGTC CCTCCAAGGT GGCATATAAG AGGTTGGTCT TGATGAACAT GCCAGTGGCC
    GAGGACATGA CCGTCCACTT CACTTCCACG CTGATGGCTC TAATCCGGAC AGCTCTGGAC
    ATTAAAATCG CCAAAGGCGG TGCAGACAGG CAGCAGCTAG ACTCAGAGCT CCAGAAGGAG
    ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTGG ATCTGCTCGT GCCCATGCCC
    AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAC
    AAACAGAGCA AGGTGAAGAA GCAGAGGCAG CAGCTGGAGG AACAGAAAAA CGCACCCATG
    TTCCAGCGCA TGGAGCCCTC GTCTCTGCCT CAGGAGATCA TCGCTAATGC CAAAGCCCTG
    CCTTATCTCC AGCAGGACCC CGTTCCAGGC CTGAGTGGCC GGAGTGCATA CCCTTCGATG
    AGTCCACTCT CCCCGCAGGA AATATTCCAG TTGGCCTGTA TGGACCCAGC TGATGATGGA
    CAGTTCCAGG AACGACAATC TCTGGAGCCA GAGGTTAGTG AATTAAAAAG CGTGCAGCCC
    TCTAACCATG GCATCTACCT TCCTTCGGAC ACCCAGGAGC ATGCGGGCTC TGGGAAGGCA
    TCCTCTATGC CACGCCTGAC TGTGGATCCC CAGGTGGTGA CAGACCCCAG CTCCATGAGA
    CGTTCATTTT CCACCATTCG CGATAAGCGT TCAAATTCCT CGTGGCTGGA GGAATTCTCC
    ATGGAGCGAA GCAGCGAAAA CACCTACAAG TCCCGGCGCC GGAGTTACCA CTCCTCCCTG
    AGGCTGTCAG CCCACCGTCT GAACTCGGAC TCAGGCCACA AGTCCGACAC CCACCGCTCA
    GGGGGCAGAG AGCGGGGACG ATCGAAAGAG CGAAAGCATC TTCTCTCTCC TGACGTCTCC
    CGCTGCAACT CCGAGGAGCG AGGGACCCAG GCCGACTGGG AGTCCCCAGA GCGCCATCAG
    TCCCGGTCTC CCAGTGAAGG CAGGTCACAG ACCCCCAACA GACAGGGCAC GGGGTCCCTG
    AGTGAGAGCT CCATCCCCTC TGTCTCCGAC TCCAGCACCC CACGACGAAG TCGGCGGCAG
    CTCCCCCCAG TCCCTCCCAA GCCTCGGCCC CTCCTTTCCT ACAGCTCTCT GATCAGACAC
    GCTGGGAGCA TCTCCCCACC AGCTGATGGG AGCGAGGGTG GCTCCCCACT GACCTCCCAA
    GCTCTGGAGA GCAACGACGC CGGCCTGCCC GAGTCTTCCA ACCCCCGGCA GGGCCACCCC
    TCCCCACAGC GCTACATCTC CGAGCCCTAC CTGGCCCTGC ACGAAGACTC CCACGCCTCG
    GACTGCGGCG AGGAGGAGAC GCTCACCTTT GAAGCAGCCG TGGCCACCAG CCTGGGCCGG
    TCCAACACCA TCGGCTCGGC CCCGCCCCTG CGGCACAGCT GGCAGATGCC CAATGGGCAC
    TATCGGCGGC GGAGGCGCGG GGGGCCTGGA CCGGGCACGA CGTGCGGAGC CGTCAGCGAC
    CTCCTGAGTG ACACGGAAGA AGACGACAAA TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811819.1
    CDS1..6936
    Translation

    Target ORF information:

    RefSeq Version XM_010813517.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813517.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG
    CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG
    GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC
    AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG
    GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA
    GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG
    CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC
    AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC
    AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG
    TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT
    GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA
    TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA
    GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG
    GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC
    GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC
    AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC
    CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC
    TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG
    GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC
    ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC
    ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC
    AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC
    TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC
    CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT
    CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG
    GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC
    TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC
    TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC
    ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA
    AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC
    ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG
    CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG
    ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT
    GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC
    CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC
    TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA
    TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG
    TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC
    ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC
    CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT
    CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC
    CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG
    ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC
    TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT
    CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG
    AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT
    GAGCCCGACA CCACTGCGCC GTCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG
    GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC
    GTGGCTGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT
    CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC
    ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCGCCGCT AGGCCTCGGC
    AAGAGATGTC CCTCCAAGGT GGCATATAAG AGGTTGGTCT TGATGAACAT GCCAGTGGCC
    GAGGACATGA CCGTCCACTT CACTTCCACG CTGATGGCTC TAATCCGGAC AGCTCTGGAC
    ATTAAAATCG CCAAAGGCGG TGCAGACAGG CAGCAGCTAG ACTCAGAGCT CCAGAAGGAG
    ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTGG ATCTGCTCGT GCCCATGCCC
    AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAC
    AAACAGAGCA AGGTGAAGAA GCAGAGGCAG CAGCTGGAGG AACAGAAAAA CGCACCCATG
    TTCCAGCGCA TGGAGCCCTC GTCTCTGCCT CAGGAGATCA TCGCTAATGC CAAAGCCCTG
    CCTTATCTCC AGCAGGACCC CGTTCCAGGC CTGAGTGGCC GGAGTGCATA CCCTTCGATG
    AGTCCACTCT CCCCGCAGGA AATATTCCAG TTGGCCTGTA TGGACCCAGC TGATGATGGA
    CAGTTCCAGG AACGACAATC TCTGGAGCCA GAGGTTAGTG AATTAAAAAG CGTGCAGCCC
    TCTAACCATG GCATCTACCT TCCTTCGGAC ACCCAGGAGC ATGCGGGCTC TGGGAAGGCA
    TCCTCTATGC CACGCCTGAC TGTGGATCCC CAGGTGGTGA CAGACCCCAG CTCCATGAGA
    CGTTCATTTT CCACCATTCG CGATAAGCGT TCAAATTCCT CGTGGCTGGA GGAATTCTCC
    ATGGAGCGAA GCAGCGAAAA CACCTACAAG TCCCGGCGCC GGAGTTACCA CTCCTCCCTG
    AGGCTGTCAG CCCACCGTCT GAACTCGGAC TCAGGCCACA AGTCCGACAC CCACCGCTCA
    GGGGGCAGAG AGCGGGGACG ATCGAAAGAG CGAAAGCATC TTCTCTCTCC TGACGTCTCC
    CGCTGCAACT CCGAGGAGCG AGGGACCCAG GCCGACTGGG AGTCCCCAGA GCGCCATCAG
    TCCCGGTCTC CCAGTGAAGG CAGGTCACAG ACCCCCAACA GACAGGGCAC GGGGTCCCTG
    AGTGAGAGCT CCATCCCCTC TGTCTCCGAC TCCAGCACCC CACGACGAAG TCGGCGGCAG
    CTCCCCCCAG TCCCTCCCAA GCCTCGGCCC CTCCTTTCCT ACAGCTCTCT GATCAGACAC
    GCTGGGAGCA TCTCCCCACC AGCTGATGGG AGCGAGGGTG GCTCCCCACT GACCTCCCAA
    GCTCTGGAGA GCAACGACGC CGGCCTGCCC GAGTCTTCCA ACCCCCGGCA GGGCCACCCC
    TCCCCACAGC GCTACATCTC CGAGCCCTAC CTGGCCCTGC ACGAAGACTC CCACGCCTCG
    GACTGCGGCG AGGAGGAGAC GCTCACCTTT GAAGCAGCCG TGGCCACCAG CCTGGGCCGG
    TCCAACACCA TCGGCTCGGC CCCGCCCCTG CGGCACAGCT GGCAGATGCC CAATGGGCAC
    TATCGGCGGC GGAGGCGCGG GGGGCCTGGA CCGGGCACGA CGTGCGGAGC CGTCAGCGAC
    CTCCTGAGTG ACACGGAAGA AGACGACAAA TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93040
    Clone ID Related Accession (Same CDS sequence) XM_015475250.1
    Accession Version XM_015475250.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG
    CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG
    GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC
    AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG
    GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA
    GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG
    CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC
    AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC
    AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG
    TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT
    GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA
    TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA
    GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG
    GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC
    GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC
    AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC
    CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC
    TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG
    GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC
    ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC
    ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC
    AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC
    TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC
    CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT
    CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG
    GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC
    TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC
    TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC
    ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA
    AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC
    ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG
    CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG
    ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT
    GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC
    CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC
    TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA
    TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG
    TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC
    ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC
    CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT
    CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC
    CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG
    ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC
    TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT
    CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG
    AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT
    GAGCCCGACA CCACTGCGCC GTCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG
    GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC
    GTGGCTGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT
    CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330736.1
    CDS846..6119
    Translation

    Target ORF information:

    RefSeq Version XM_015475250.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475250.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG
    CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG
    GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC
    AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG
    GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA
    GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG
    CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC
    AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC
    AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG
    TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT
    GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA
    TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA
    GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG
    GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC
    GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC
    AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC
    CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC
    TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG
    GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC
    ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC
    ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC
    AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC
    TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC
    CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT
    CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG
    GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC
    TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC
    TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC
    ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA
    AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC
    ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG
    CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG
    ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT
    GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC
    CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC
    TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA
    TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG
    TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC
    ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC
    CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT
    CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC
    CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG
    ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC
    TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT
    CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG
    AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT
    GAGCCCGACA CCACTGCGCC GTCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG
    GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC
    GTGGCTGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT
    CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216174
    Clone ID Related Accession (Same CDS sequence) NM_001191123.2
    Accession Version NM_001191123.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product voltage-dependent R-type calcium channel subunit alpha-1E
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02043534.1, DAAA02043538.1 and DAAA02043541.1. On Jun 29, 2017 this sequence version replaced NM_001191123.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092, SAMN02822093 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG
    CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG
    GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC
    AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG
    GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA
    GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG
    CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC
    AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC
    AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG
    TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT
    GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA
    TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA
    GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG
    GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC
    GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC
    AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC
    CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC
    TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG
    GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC
    ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC
    ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC
    AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC
    TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC
    CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT
    CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG
    GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC
    TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC
    TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC
    ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA
    AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC
    ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG
    CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG
    ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT
    GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC
    CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC
    TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA
    TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG
    TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC
    ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC
    CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT
    CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC
    CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG
    ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC
    TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT
    CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG
    AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT
    GAGCCCGACA CCACTGCGCC GTCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG
    GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC
    GTGGCTGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT
    CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC
    ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCGCCGCT AGGCCTCGGC
    AAGAGATGTC CCTCCAAGGT GGCATATAAG AGGTTGGTCT TGATGAACAT GCCAGTGGCC
    GAGGACATGA CCGTCCACTT CACTTCCACG CTGATGGCTC TAATCCGGAC AGCTCTGGAC
    ATTAAAATCG CCAAAGGCGG TGCAGACAGG CAGCAGCTAG ACTCAGAGCT CCAGAAGGAG
    ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTGG ATCTGCTCGT GCCCATGCCC
    AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAC
    AAACAGAGCA AGGTGAAGAA GCAGAGGCAG CAGCTGGAGG AACAGAAAAA CGCACCCATG
    TTCCAGCGCA TGGAGCCCTC GTCTCTGCCT CAGGAGATCA TCGCTAATGC CAAAGCCCTG
    CCTTATCTCC AGCAGGACCC CGTTCCAGGC CTGAGTGGCC GGAGTGCATA CCCTTCGATG
    AGTCCACTCT CCCCGCAGGA AATATTCCAG TTGGCCTGTA TGGACCCAGC TGATGATGGA
    CAGTTCCAGG AACGACAATC TCTGGTGGTG ACAGACCCCA GCTCCATGAG ACGTTCATTT
    TCCACCATTC GCGATAAGCG TTCAAATTCC TCGTGGCTGG AGGAATTCTC CATGGAGCGA
    AGCAGCGAAA ACACCTACAA GTCCCGGCGC CGGAGTTACC ACTCCTCCCT GAGGCTGTCA
    GCCCACCGTC TGAACTCGGA CTCAGGCCAC AAGTCCGACA CCCACCGCTC AGGGGGCAGA
    GAGCGGGGAC GATCGAAAGA GCGAAAGCAT CTTCTCTCTC CTGACGTCTC CCGCTGCAAC
    TCCGAGGAGC GAGGGACCCA GGCCGACTGG GAGTCCCCAG AGCGCCATCA GTCCCGGTCT
    CCCAGTGAAG GCAGGTCACA GACCCCCAAC AGACAGGGCA CGGGGTCCCT GAGTGAGAGC
    TCCATCCCCT CTGTCTCCGA CTCCAGCACC CCACGACGAA GTCGGCGGCA GCTCCCCCCA
    GTCCCTCCCA AGCCTCGGCC CCTCCTTTCC TACAGCTCTC TGATCAGACA CGCTGGGAGC
    ATCTCCCCAC CAGCTGATGG GAGCGAGGGT GGCTCCCCAC TGACCTCCCA AGCTCTGGAG
    AGCAACGACG CCGGCCTGCC CGAGTCTTCC AACCCCCGGC AGGGCCACCC CTCCCCACAG
    CGCTACATCT CCGAGCCCTA CCTGGCCCTG CACGAAGACT CCCACGCCTC GGACTGCGGC
    GAGGAGGAGA CGCTCACCTT TGAAGCAGCC GTGGCCACCA GCCTGGGCCG GTCCAACACC
    ATCGGCTCGG CCCCGCCCCT GCGGCACAGC TGGCAGATGC CCAATGGGCA CTATCGGCGG
    CGGAGGCGCG GGGGGCCTGG ACCGGGCACG ACGTGCGGAG CCGTCAGCGA CCTCCTGAGT
    GACACGGAAG AAGACGACAA ATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178052.1
    CDS1..6807
    Translation

    Target ORF information:

    RefSeq Version NM_001191123.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191123.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG
    CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG
    GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC
    AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG
    GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA
    GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG
    CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC
    AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC
    AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG
    TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT
    GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA
    TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA
    GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG
    GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC
    GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC
    AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC
    CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC
    TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG
    GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC
    ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC
    ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC
    AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC
    TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC
    CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT
    CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG
    GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC
    TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC
    TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC
    ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA
    AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC
    ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG
    CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG
    ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT
    GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC
    CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC
    TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA
    TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG
    TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC
    ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC
    CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT
    CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC
    CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG
    ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC
    TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT
    CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG
    AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT
    GAGCCCGACA CCACTGCGCC GTCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG
    GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC
    GTGGCTGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT
    CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC
    ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCGCCGCT AGGCCTCGGC
    AAGAGATGTC CCTCCAAGGT GGCATATAAG AGGTTGGTCT TGATGAACAT GCCAGTGGCC
    GAGGACATGA CCGTCCACTT CACTTCCACG CTGATGGCTC TAATCCGGAC AGCTCTGGAC
    ATTAAAATCG CCAAAGGCGG TGCAGACAGG CAGCAGCTAG ACTCAGAGCT CCAGAAGGAG
    ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTGG ATCTGCTCGT GCCCATGCCC
    AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAC
    AAACAGAGCA AGGTGAAGAA GCAGAGGCAG CAGCTGGAGG AACAGAAAAA CGCACCCATG
    TTCCAGCGCA TGGAGCCCTC GTCTCTGCCT CAGGAGATCA TCGCTAATGC CAAAGCCCTG
    CCTTATCTCC AGCAGGACCC CGTTCCAGGC CTGAGTGGCC GGAGTGCATA CCCTTCGATG
    AGTCCACTCT CCCCGCAGGA AATATTCCAG TTGGCCTGTA TGGACCCAGC TGATGATGGA
    CAGTTCCAGG AACGACAATC TCTGGTGGTG ACAGACCCCA GCTCCATGAG ACGTTCATTT
    TCCACCATTC GCGATAAGCG TTCAAATTCC TCGTGGCTGG AGGAATTCTC CATGGAGCGA
    AGCAGCGAAA ACACCTACAA GTCCCGGCGC CGGAGTTACC ACTCCTCCCT GAGGCTGTCA
    GCCCACCGTC TGAACTCGGA CTCAGGCCAC AAGTCCGACA CCCACCGCTC AGGGGGCAGA
    GAGCGGGGAC GATCGAAAGA GCGAAAGCAT CTTCTCTCTC CTGACGTCTC CCGCTGCAAC
    TCCGAGGAGC GAGGGACCCA GGCCGACTGG GAGTCCCCAG AGCGCCATCA GTCCCGGTCT
    CCCAGTGAAG GCAGGTCACA GACCCCCAAC AGACAGGGCA CGGGGTCCCT GAGTGAGAGC
    TCCATCCCCT CTGTCTCCGA CTCCAGCACC CCACGACGAA GTCGGCGGCA GCTCCCCCCA
    GTCCCTCCCA AGCCTCGGCC CCTCCTTTCC TACAGCTCTC TGATCAGACA CGCTGGGAGC
    ATCTCCCCAC CAGCTGATGG GAGCGAGGGT GGCTCCCCAC TGACCTCCCA AGCTCTGGAG
    AGCAACGACG CCGGCCTGCC CGAGTCTTCC AACCCCCGGC AGGGCCACCC CTCCCCACAG
    CGCTACATCT CCGAGCCCTA CCTGGCCCTG CACGAAGACT CCCACGCCTC GGACTGCGGC
    GAGGAGGAGA CGCTCACCTT TGAAGCAGCC GTGGCCACCA GCCTGGGCCG GTCCAACACC
    ATCGGCTCGG CCCCGCCCCT GCGGCACAGC TGGCAGATGC CCAATGGGCA CTATCGGCGG
    CGGAGGCGCG GGGGGCCTGG ACCGGGCACG ACGTGCGGAG CCGTCAGCGA CCTCCTGAGT
    GACACGGAAG AAGACGACAA ATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238960
    Clone ID Related Accession (Same CDS sequence) NM_001191123.3
    Accession Version NM_001191123.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product voltage-dependent R-type calcium channel subunit alpha-1E
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000016.1. On May 29, 2018 this sequence version replaced NM_001191123.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145414, SAMN03145415 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG
    CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG
    GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC
    AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG
    GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA
    GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG
    CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC
    AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC
    AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG
    TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT
    GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA
    TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA
    GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG
    GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC
    GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC
    AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC
    CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC
    TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG
    GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC
    ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC
    ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC
    AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC
    TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC
    CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT
    CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG
    GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC
    TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC
    TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC
    ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA
    AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC
    ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG
    CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG
    ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT
    GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC
    CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC
    TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA
    TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG
    TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC
    ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC
    CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT
    CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC
    CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG
    ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC
    TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT
    CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG
    AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT
    GAGCCCGACA CCACTGCGCC GTCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG
    GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC
    GTGGCTGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT
    CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC
    ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCGCCGCT AGGCCTCGGC
    AAGAGATGTC CCTCCAAGGT GGCATATAAG AGGTTGGTCT TGATGAACAT GCCAGTGGCC
    GAGGACATGA CCGTCCACTT CACTTCCACG CTGATGGCTC TAATCCGGAC AGCTCTGGAC
    ATTAAAATCG CCAAAGGCGG TGCAGACAGG CAGCAGCTAG ACTCAGAGCT CCAGAAGGAG
    ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTGG ATCTGCTCGT GCCCATGCCC
    AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAC
    AAACAGAGCA AGGTGAAGAA GCAGAGGCAG CAGCTGGAGG AACAGAAAAA CGCACCCATG
    TTCCAGCGCA TGGAGCCCTC GTCTCTGCCT CAGGAGATCA TCGCTAATGC CAAAGCCCTG
    CCTTATCTCC AGCAGGACCC CGTTCCAGGC CTGAGTGGCC GGAGTGCATA CCCTTCGATG
    AGTCCACTCT CCCCGCAGGA AATATTCCAG TTGGCCTGTA TGGACCCAGC TGATGATGGA
    CAGTTCCAGG AACGACAATC TCTGGTGGTG ACAGACCCCA GCTCCATGAG ACGTTCATTT
    TCCACCATTC GCGATAAGCG TTCAAATTCC TCGTGGCTGG AGGAATTCTC CATGGAGCGA
    AGCAGCGAAA ACACCTACAA GTCCCGGCGC CGGAGTTACC ACTCCTCCCT GAGGCTGTCA
    GCCCACCGTC TGAACTCGGA CTCAGGCCAC AAGTCCGACA CCCACCGCTC AGGGGGCAGA
    GAGCGGGGAC GATCGAAAGA GCGAAAGCAT CTTCTCTCTC CTGACGTCTC CCGCTGCAAC
    TCCGAGGAGC GAGGGACCCA GGCCGACTGG GAGTCCCCAG AGCGCCATCA GTCCCGGTCT
    CCCAGTGAAG GCAGGTCACA GACCCCCAAC AGACAGGGCA CGGGGTCCCT GAGTGAGAGC
    TCCATCCCCT CTGTCTCCGA CTCCAGCACC CCACGACGAA GTCGGCGGCA GCTCCCCCCA
    GTCCCTCCCA AGCCTCGGCC CCTCCTTTCC TACAGCTCTC TGATCAGACA CGCTGGGAGC
    ATCTCCCCAC CAGCTGATGG GAGCGAGGGT GGCTCCCCAC TGACCTCCCA AGCTCTGGAG
    AGCAACGACG CCGGCCTGCC CGAGTCTTCC AACCCCCGGC AGGGCCACCC CTCCCCACAG
    CGCTACATCT CCGAGCCCTA CCTGGCCCTG CACGAAGACT CCCACGCCTC GGACTGCGGC
    GAGGAGGAGA CGCTCACCTT TGAAGCAGCC GTGGCCACCA GCCTGGGCCG GTCCAACACC
    ATCGGCTCGG CCCCGCCCCT GCGGCACAGC TGGCAGATGC CCAATGGGCA CTATCGGCGG
    CGGAGGCGCG GGGGGCCTGG ACCGGGCACG ACGTGCGGAG CCGTCAGCGA CCTCCTGAGT
    GACACGGAAG AAGACGACAA ATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178052.1
    CDS1..6807
    Translation

    Target ORF information:

    RefSeq Version NM_001191123.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191123.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG
    CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG
    GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC
    AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG
    GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA
    GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG
    CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC
    AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC
    AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG
    TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT
    GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA
    TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA
    GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG
    GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC
    GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC
    AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC
    CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC
    TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG
    GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC
    ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC
    ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC
    AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC
    TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC
    CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT
    CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG
    GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC
    TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC
    TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC
    ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA
    AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC
    ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG
    CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG
    ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT
    GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC
    CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC
    TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA
    TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG
    TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC
    ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC
    CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT
    CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC
    CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG
    ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC
    TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT
    CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG
    AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT
    GAGCCCGACA CCACTGCGCC GTCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG
    GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC
    GTGGCTGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT
    CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC
    ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCGCCGCT AGGCCTCGGC
    AAGAGATGTC CCTCCAAGGT GGCATATAAG AGGTTGGTCT TGATGAACAT GCCAGTGGCC
    GAGGACATGA CCGTCCACTT CACTTCCACG CTGATGGCTC TAATCCGGAC AGCTCTGGAC
    ATTAAAATCG CCAAAGGCGG TGCAGACAGG CAGCAGCTAG ACTCAGAGCT CCAGAAGGAG
    ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTGG ATCTGCTCGT GCCCATGCCC
    AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAC
    AAACAGAGCA AGGTGAAGAA GCAGAGGCAG CAGCTGGAGG AACAGAAAAA CGCACCCATG
    TTCCAGCGCA TGGAGCCCTC GTCTCTGCCT CAGGAGATCA TCGCTAATGC CAAAGCCCTG
    CCTTATCTCC AGCAGGACCC CGTTCCAGGC CTGAGTGGCC GGAGTGCATA CCCTTCGATG
    AGTCCACTCT CCCCGCAGGA AATATTCCAG TTGGCCTGTA TGGACCCAGC TGATGATGGA
    CAGTTCCAGG AACGACAATC TCTGGTGGTG ACAGACCCCA GCTCCATGAG ACGTTCATTT
    TCCACCATTC GCGATAAGCG TTCAAATTCC TCGTGGCTGG AGGAATTCTC CATGGAGCGA
    AGCAGCGAAA ACACCTACAA GTCCCGGCGC CGGAGTTACC ACTCCTCCCT GAGGCTGTCA
    GCCCACCGTC TGAACTCGGA CTCAGGCCAC AAGTCCGACA CCCACCGCTC AGGGGGCAGA
    GAGCGGGGAC GATCGAAAGA GCGAAAGCAT CTTCTCTCTC CTGACGTCTC CCGCTGCAAC
    TCCGAGGAGC GAGGGACCCA GGCCGACTGG GAGTCCCCAG AGCGCCATCA GTCCCGGTCT
    CCCAGTGAAG GCAGGTCACA GACCCCCAAC AGACAGGGCA CGGGGTCCCT GAGTGAGAGC
    TCCATCCCCT CTGTCTCCGA CTCCAGCACC CCACGACGAA GTCGGCGGCA GCTCCCCCCA
    GTCCCTCCCA AGCCTCGGCC CCTCCTTTCC TACAGCTCTC TGATCAGACA CGCTGGGAGC
    ATCTCCCCAC CAGCTGATGG GAGCGAGGGT GGCTCCCCAC TGACCTCCCA AGCTCTGGAG
    AGCAACGACG CCGGCCTGCC CGAGTCTTCC AACCCCCGGC AGGGCCACCC CTCCCCACAG
    CGCTACATCT CCGAGCCCTA CCTGGCCCTG CACGAAGACT CCCACGCCTC GGACTGCGGC
    GAGGAGGAGA CGCTCACCTT TGAAGCAGCC GTGGCCACCA GCCTGGGCCG GTCCAACACC
    ATCGGCTCGG CCCCGCCCCT GCGGCACAGC TGGCAGATGC CCAATGGGCA CTATCGGCGG
    CGGAGGCGCG GGGGGCCTGG ACCGGGCACG ACGTGCGGAG CCGTCAGCGA CCTCCTGAGT
    GACACGGAAG AAGACGACAA ATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223446
    Clone ID Related Accession (Same CDS sequence) XM_024976061.1
    Accession Version XM_024976061.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGATGTGGG TTGATGAAGT TTGTGTATGG ATGTACATAG CCATCTTGGA GGCTGTCCTT 
    CTCTCTTTGG TGATTCTGTT TCTTAACTCC TCCTTTCCCA CGCCGTTTGA GTACATGATC
    TTGGCCACCA TCATTGCCAA TTGCATCGTC TTGGCCCTGG AGCAACATCT TCCTGAGGAT
    GACAAGACCC CAATGTCCCG CAGACTGGAG AAGACAGAAC CCTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATCGTGGCC CTGGGGTTCA TCTTCCATAA GGGATCATAC
    CTCCGCAATG GTTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCTACT
    GCGGGAACCC ACTTCAACAC CCATGTGGAC CTGAGGACCC TCCGGGCCGT GCGTGTCCTG
    AGGCCCCTGA AGCTCGTGTC GGGGATACCT AGCCTACAGA TTGTGTTGAA ATCCATCATG
    AAGGCCATGG TGCCTCTCCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATCG GCTTGGAGTT CTACAGTGGA AAGTTGCACC GAGCGTGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GCGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCCGGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCCG TGCTGACCGT CTTCCAGTGT ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTT GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGGAGA GCCTTCATGA AACTGCGGCG CCAGCAGCAG
    ATCGAGCGTG AGCTTAACGG GTACCGCGCC TGGATAGACA AAGCAGAGGA AGTCATGCTT
    GCTGAAGAAA ACAAAAATGC TGGAACGTCA GCCTTAGAAG TGCTTCGGAG GGCAACCATC
    AAAAGGAGCC GGACCGAGGC CATGGCACGG GACTCCAGCG ATGAGCACTG CGTGGACATC
    TCCTCTGTGG GCACACCTCT CGCCCGAGCC AGCATCAAGA GCGCCAAGGT GGACGGGGCC
    TCCTATTTCC GGCACAAGGA GCGACTTTTG CGGATCTCTA TCCGCCACAT GGTTAAGTCC
    CAGGTGTTCT ACTGGATCGT GCTGAGCCTG GTGGCTCTCA ACACCGCCTG TGTGGCCATC
    GTCCACCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA GTTTCTGTTT
    CTGGGGCTCT TCCTTCTAGA GATGTCCCTG AAGATGTACG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CCTTCAACTG CTTTGACTTT GGGGTCACAG TGGGCAGCAT CTTTGAAGTA
    GTCTGGGCCA TCTTCAGACC TGGTACATCT TTCGGAATCA GTGTCCTGCG AGCTCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCATCCC TCCGGAATTT GGTGGTCTCC
    TTGATGAGTT CCATGAAGTC TATCATCAGT TTACTCTTCC TCCTTTTCCT CTTCATTGTT
    GTCTTTGCTC TGCTAGGAAT GCAGCTGTTT GGGGGCAGGT TCAACTTTAA TGATGGGACT
    CCTTCCGCAA ACTTTGACAC CTTCCCTGCA GCCATCATGA CTGTATTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAC GGGATCCGCT CCCAGGGTGG GGTCAGCTCG
    GGCATGTGGT CTGCGATCTA TTTCATTGTG CTTACCTTGT TTGGAAACTA CACACTGCTG
    AATGTGTTCT TGGCCATCGC TGTGGACAAT CTGGCCAACG CCCAGGAGCT GACCAAGGAT
    GAACAAGAAG AAGAGGAGGC CTTCAACCAG AAGCATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCC ATCGAGAGGG AGCGGAGGCG CCGGCATCAC
    ATGTCCGTGT GGGAGCAGCG AACCAGCCAG TTGAGGAGGC ACATGCAGAT GTCCAGCCAG
    GAGGCCCTCA ACAAGGAGGA GGCCCCGCCC ATGAACCCCC TCAACCCCCT CAACCCGCTG
    AGCCCCCTCA ACCCGCTCAG CGCGCACCCC AGCCTCTACC GGAGACCCCG GGCCATCGAG
    GGGCTAGCCC TGGGCCTGGG GCTGGAGAAG TGTGAGGAAG AGCGCGTCAG CCGTGGGGGG
    TCCCTCAAAG GGGATGGAGG GGACTTGGCG AGCGCCCTGG ACAGCCAGAG GAGCCCCCTG
    GCCCTGGGCA AACGGGAGCC ACCCTGGCTG GCCCGGCCCT GTCACGGGAA CTGTGACCCG
    ACCCAGCCGG AGGCGGGCAG TGGGGAGACG GTGGTGACCT TCGGGGACCG GGCCCGGCAC
    AGGCAGAGCC AGCGGCGCAG CCGGCACCGC CGCGTCAGGA CCGAGGGCAG GGAGCCCTCC
    TCCACCTCCA GAAGCAGGTC TGCCAGCCAG GAGCGGAGTC TGGACGAAGC TGTGCCCAGC
    GAGGGGGAGA GGGACCGTGA GCCCAGGGCT GGCCATGGGG GCAAAGAACC CATGATCCAG
    GAGGAAGAGC GAGAGCGATG CCAGGACTTA CGGAGGACCA ACAGTCTGAT GGTGCCCAGA
    GGCTCCGGGC TGGCAGGAGC CCTGGATGAG GCCGACACAC CCCTTGTCCC ACCCCAGCCG
    GAGCTGGAAG TGGGGAAGGA CGCAGCCCTG ACGGAGCAGG AGCCGGAGGG CAGCAGTGAG
    CAGGCTCTGC TGGGGGACGT GAAGCTGGAC ATAGGCCGGG TCAGCCAGAG CGAGCCCGAC
    CTCTCCTGCG TCACGGCCAA CACGGACAAG GCCACCACCG AGAGCACCAG CGTCACTGTC
    ACCATCCCCG ACGTGGGCCC CTTGGTGGAC TCAACCGTGG TGCACATCAG CAACAAGACT
    GATGGAGAAG CCAGTCCCTT GAAAGAGGCA GAGATCAGAG AGGATGAGGA GGAGGTGGAG
    AAGAAGCAGC AGAAGAAGGA GAAGCGTGAG ACGGGCAAGG CCATGGTGCC CCACAGCTCG
    ATGTTCATCT TCAGCACCAC CAACCCGATC CGCAGGGCCT GCCACTACAT CGTGAACCTG
    CGCTACTTCG AGATGTGCAT CCTCCTGGTG ATCGCAGCCA GCAGCATCGC CCTGGCGGCC
    GAGGACCCCG TCCTGACCAA CTCAGAGCGC AACAAAGTGC TGAGGTATTT TGACTATGTG
    TTCACTGGCG TGTTCACCTT TGAGATGGTT ATAAAGATGA TAGACCAGGG CTTGATTCTG
    CAGGACGGCT CCTACTTCCG AGACCTGTGG AACATCCTGG ACTTCGTGGT GGTGGTTGGC
    GCATTGGTGG CCTTTGCTCT GGCAACTAAC AAGGGCCGGG ATATTAAGAC CATCAAGTCC
    CTGAGGGTTC TCCGTGTCCT GAGGCCGCTG AAGACCATCA AGCGCCTGCC CAAGCTCAAG
    GCTGTCTTTG ACTGTGTGGT CACCTCCTTG AAAAACGTCT TCAACATACT CATTGTCTAC
    AAGCTCTTCA TGTTCATCTT TGCTGTCATC GCAGTTCAGC TTTTCAAGGG CAAGTTCTTC
    TACTGCACGG ACAGTTCCAA GGACACAGAG AAGGAGTGCA TAGGCAACTA TGTAGATCAC
    GAGAAAAACA AGATGGAGGT GAAAGGCCGA GAGTGGAAGC GCCATGAATT CCATTATGAC
    AACATCATCT GGGCTCTGCT GACCCTCTTC ACTGTCTCCA CAGGGGAAGG GTGGCCTCAG
    GTCTTGCAGC ACTCGGTCGA TGTGACAGAG GAAGACCGAG GCCCAAGCCG CAGCAACCGG
    ATGGAGATGT CCATCTTTTA TGTGGTCTAC TTTGTGGTCT TCCCCTTCTT CTTTGTCAAC
    ATCTTTGTGG CTCTCATCAT CATCACCTTC CAGGAGCAAG GGGACAAGAT GATGGAAGAG
    TGCAGCCTGG AGAAGAATGA GAGGGCATGC ATCGACTTCG CCATCAGCGC CAAACCGCTC
    ACCCGCTACA TGCCCCAGAA CAGGCACACC TTCCAGTACC GCGTCTGGCA CTTCGTGGTG
    TCTCCATCCT TTGAGTACAC CATCATGGCC ATGATCGCCC TGAACACCGT CGTGCTGATG
    ATGAAGTACT ACTCTGCTCC CTGTACCTAT GAGCTGGCCC TGAAGTACCT AAATATCGCC
    TTCACCATGG TGTTTTCCCT GGAATGTGTC CTGAAGATCA TTGCATTTGG CTTCTTGAAC
    TATTTCCGAG ACACCTGGAA TATCTTTGAC TTCATCACTG TGATTGGCAG TATCACAGAA
    ATTATTCTGA CGGACAGCAA GCTGGTGAAC ACTAGTGGCT TCAACATGAG CTTTCTGAAG
    CTCTTCCGAG CGGCCCGCCT CATCAAGCTG CTGCGTCAGG GCTACACCAT CCGCATTTTA
    CTGTGGACCT TCGTGCAGTC CTTTAAGGCC CTTCCCTATG TCTGCCTTCT GATCGCCATG
    CTTTTCTTCA TCTATGCCAT CATTGGGATG CAGGTCTTTG GAAACATAAA GTTAGATGAG
    GAGAGTCACA TCAACCGGCA CAACAACTTC CGGAGTTTCT TTGGGTCCCT CATGCTGCTC
    TTCAGGAGTG CCACGGGTGA GGCCTGGCAG GAGATAATGC TGTCGTGTCT CGGGGAGAAG
    GGCTGTGAGC CCGACACCAC TGCGCCGTCG GGGCAGAGCG AGAGCGAGCG CTGTGGCACC
    GACCTGGCCT ACGTGTACTT CGTCTCCTTC ATCTTCTTCT GCTCCTTCCT GATGCTCAAC
    CTGTTCGTGG CTGTCATCAT GGACAACTTT GAGTACCTGA CTCGGGACTC GTCCATCCTG
    GGGCCTCACC ATCTGGACGA GTTTGTCCGA GTCTGGGCAG AGTATGACAG AGCAGCATGT
    GGCCGCATCC ATTACACTGA GATGTATGAA ATGCTGACTC TCATGTCACC GCCGCTAGGC
    CTCGGCAAGA GATGTCCCTC CAAGGTGGCA TATAAGAGGT TGGTCTTGAT GAACATGCCA
    GTGGCCGAGG ACATGACCGT CCACTTCACT TCCACGCTGA TGGCTCTAAT CCGGACAGCT
    CTGGACATTA AAATCGCCAA AGGCGGTGCA GACAGGCAGC AGCTAGACTC AGAGCTCCAG
    AAGGAGACCC TGGCCATCTG GCCTCACCTG TCCCAGAAGA TGCTGGATCT GCTCGTGCCC
    ATGCCCAAAG CCTCTGACCT GACTGTGGGC AAAATCTATG CAGCAATGAT GATCATGGAC
    TACTACAAAC AGAGCAAGGT GAAGAAGCAG AGGCAGCAGC TGGAGGAACA GAAAAACGCA
    CCCATGTTCC AGCGCATGGA GCCCTCGTCT CTGCCTCAGG AGATCATCGC TAATGCCAAA
    GCCCTGCCTT ATCTCCAGCA GGACCCCGTT CCAGGCCTGA GTGGCCGGAG TGCATACCCT
    TCGATGAGTC CACTCTCCCC GCAGGAAATA TTCCAGTTGG CCTGTATGGA CCCAGCTGAT
    GATGGACAGT TCCAGGAACG ACAATCTCTG GAGCCAGAGG TTAGTGAATT AAAAAGCGTG
    CAGCCCTCTA ACCATGGCAT CTACCTTCCT TCGGACACCC AGGAGCATGC GGGCTCTGGG
    AAGGCATCCT CTATGCCACG CCTGACTGTG GATCCCCAGG TGGTGACAGA CCCCAGCTCC
    ATGAGACGTT CATTTTCCAC CATTCGCGAT AAGCGTTCAA ATTCCTCGTG GCTGGAGGAA
    TTCTCCATGG AGCGAAGCAG CGAAAACACC TACAAGTCCC GGCGCCGGAG TTACCACTCC
    TCCCTGAGGC TGTCAGCCCA CCGTCTGAAC TCGGACTCAG GCCACAAGTC CGACACCCAC
    CGCTCAGGGG GCAGAGAGCG GGGACGATCG AAAGAGCGAA AGCATCTTCT CTCTCCTGAC
    GTCTCCCGCT GCAACTCCGA GGAGCGAGGG ACCCAGGCCG ACTGGGAGTC CCCAGAGCGC
    CATCAGTCCC GGTCTCCCAG TGAAGGCAGG TCACAGACCC CCAACAGACA GGGCACGGGG
    TCCCTGAGTG AGAGCTCCAT CCCCTCTGTC TCCGACTCCA GCACCCCACG ACGAAGTCGG
    CGGCAGCTCC CCCCAGTCCC TCCCAAGCCT CGGCCCCTCC TTTCCTACAG CTCTCTGATC
    AGACACGCTG GGAGCATCTC CCCACCAGCT GATGGGAGCG AGGGTGGCTC CCCACTGACC
    TCCCAAGCTC TGGAGAGCAA CGACGCCGGC CTGCCCGAGT CTTCCAACCC CCGGCAGGGC
    CACCCCTCCC CACAGCGCTA CATCTCCGAG CCCTACCTGG CCCTGCACGA AGACTCCCAC
    GCCTCGGACT GCGGCGAGGA GGAGACGCTC ACCTTTGAAG CAGCCGTGGC CACCAGCCTG
    GGCCGGTCCA ACACCATCGG CTCGGCCCCG CCCCTGCGGC ACAGCTGGCA GATGCCCAAT
    GGGCACTATC GGCGGCGGAG GCGCGGGGGG CCTGGACCGG GCACGACGTG CGGAGCCGTC
    AGCGACCTCC TGAGTGACAC GGAAGAAGAC GACAAATGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831829.1
    CDS7..6708
    Translation

    Target ORF information:

    RefSeq Version XM_024976061.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976061.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGATGTGGG TTGATGAAGT TTGTGTATGG ATGTACATAG CCATCTTGGA GGCTGTCCTT 
    CTCTCTTTGG TGATTCTGTT TCTTAACTCC TCCTTTCCCA CGCCGTTTGA GTACATGATC
    TTGGCCACCA TCATTGCCAA TTGCATCGTC TTGGCCCTGG AGCAACATCT TCCTGAGGAT
    GACAAGACCC CAATGTCCCG CAGACTGGAG AAGACAGAAC CCTATTTCAT TGGGATCTTT
    TGCTTTGAAG CTGGGATCAA AATCGTGGCC CTGGGGTTCA TCTTCCATAA GGGATCATAC
    CTCCGCAATG GTTGGAATGT CATGGACTTC ATCGTGGTCC TCAGTGGCAT CCTGGCTACT
    GCGGGAACCC ACTTCAACAC CCATGTGGAC CTGAGGACCC TCCGGGCCGT GCGTGTCCTG
    AGGCCCCTGA AGCTCGTGTC GGGGATACCT AGCCTACAGA TTGTGTTGAA ATCCATCATG
    AAGGCCATGG TGCCTCTCCT GCAGATTGGC CTTCTGCTCT TCTTTGCCAT CCTGATGTTT
    GCTATCATCG GCTTGGAGTT CTACAGTGGA AAGTTGCACC GAGCGTGCTT CATGAACAAT
    TCAGGTATTC TAGAAGGATT TGACCCCCCT CACCCATGTG GCGTGCAGGG CTGCCCAGCT
    GGTTATGAAT GCCGGGACTG GATCGGCCCC AATGATGGGA TCACCCAGTT TGATAACATC
    CTTTTTGCCG TGCTGACCGT CTTCCAGTGT ATCACCATGG AAGGGTGGAC CACTGTGCTG
    TACAATACCA ATGATGCCTT AGGAGCCACC TGGAATTGGC TGTACTTCAT CCCCCTCATC
    ATCATTGGAT CCTTCTTTGT TCTCAACCTT GTCCTGGGAG TGCTTTCCGG GGAATTTGCC
    AAAGAGAGAG AGAGAGTGGA GAACCGGAGA GCCTTCATGA AACTGCGGCG CCAGCAGCAG
    ATCGAGCGTG AGCTTAACGG GTACCGCGCC TGGATAGACA AAGCAGAGGA AGTCATGCTT
    GCTGAAGAAA ACAAAAATGC TGGAACGTCA GCCTTAGAAG TGCTTCGGAG GGCAACCATC
    AAAAGGAGCC GGACCGAGGC CATGGCACGG GACTCCAGCG ATGAGCACTG CGTGGACATC
    TCCTCTGTGG GCACACCTCT CGCCCGAGCC AGCATCAAGA GCGCCAAGGT GGACGGGGCC
    TCCTATTTCC GGCACAAGGA GCGACTTTTG CGGATCTCTA TCCGCCACAT GGTTAAGTCC
    CAGGTGTTCT ACTGGATCGT GCTGAGCCTG GTGGCTCTCA ACACCGCCTG TGTGGCCATC
    GTCCACCACA ACCAGCCCCA GTGGCTCACC CACCTCCTCT ACTATGCAGA GTTTCTGTTT
    CTGGGGCTCT TCCTTCTAGA GATGTCCCTG AAGATGTACG GCATGGGGCC TCGCCTTTAT
    TTTCACTCTT CCTTCAACTG CTTTGACTTT GGGGTCACAG TGGGCAGCAT CTTTGAAGTA
    GTCTGGGCCA TCTTCAGACC TGGTACATCT TTCGGAATCA GTGTCCTGCG AGCTCTCCGG
    CTTCTAAGAA TATTTAAAAT AACCAAGTAT TGGGCATCCC TCCGGAATTT GGTGGTCTCC
    TTGATGAGTT CCATGAAGTC TATCATCAGT TTACTCTTCC TCCTTTTCCT CTTCATTGTT
    GTCTTTGCTC TGCTAGGAAT GCAGCTGTTT GGGGGCAGGT TCAACTTTAA TGATGGGACT
    CCTTCCGCAA ACTTTGACAC CTTCCCTGCA GCCATCATGA CTGTATTCCA GATCCTGACG
    GGTGAGGACT GGAATGAGGT GATGTACAAC GGGATCCGCT CCCAGGGTGG GGTCAGCTCG
    GGCATGTGGT CTGCGATCTA TTTCATTGTG CTTACCTTGT TTGGAAACTA CACACTGCTG
    AATGTGTTCT TGGCCATCGC TGTGGACAAT CTGGCCAACG CCCAGGAGCT GACCAAGGAT
    GAACAAGAAG AAGAGGAGGC CTTCAACCAG AAGCATGCAC TGCAGAAGGC CAAGGAGGTC
    AGCCCGATGT CTGCACCCAA CATGCCTTCC ATCGAGAGGG AGCGGAGGCG CCGGCATCAC
    ATGTCCGTGT GGGAGCAGCG AACCAGCCAG TTGAGGAGGC ACATGCAGAT GTCCAGCCAG
    GAGGCCCTCA ACAAGGAGGA GGCCCCGCCC ATGAACCCCC TCAACCCCCT CAACCCGCTG
    AGCCCCCTCA ACCCGCTCAG CGCGCACCCC AGCCTCTACC GGAGACCCCG GGCCATCGAG
    GGGCTAGCCC TGGGCCTGGG GCTGGAGAAG TGTGAGGAAG AGCGCGTCAG CCGTGGGGGG
    TCCCTCAAAG GGGATGGAGG GGACTTGGCG AGCGCCCTGG ACAGCCAGAG GAGCCCCCTG
    GCCCTGGGCA AACGGGAGCC ACCCTGGCTG GCCCGGCCCT GTCACGGGAA CTGTGACCCG
    ACCCAGCCGG AGGCGGGCAG TGGGGAGACG GTGGTGACCT TCGGGGACCG GGCCCGGCAC
    AGGCAGAGCC AGCGGCGCAG CCGGCACCGC CGCGTCAGGA CCGAGGGCAG GGAGCCCTCC
    TCCACCTCCA GAAGCAGGTC TGCCAGCCAG GAGCGGAGTC TGGACGAAGC TGTGCCCAGC
    GAGGGGGAGA GGGACCGTGA GCCCAGGGCT GGCCATGGGG GCAAAGAACC CATGATCCAG
    GAGGAAGAGC GAGAGCGATG CCAGGACTTA CGGAGGACCA ACAGTCTGAT GGTGCCCAGA
    GGCTCCGGGC TGGCAGGAGC CCTGGATGAG GCCGACACAC CCCTTGTCCC ACCCCAGCCG
    GAGCTGGAAG TGGGGAAGGA CGCAGCCCTG ACGGAGCAGG AGCCGGAGGG CAGCAGTGAG
    CAGGCTCTGC TGGGGGACGT GAAGCTGGAC ATAGGCCGGG TCAGCCAGAG CGAGCCCGAC
    CTCTCCTGCG TCACGGCCAA CACGGACAAG GCCACCACCG AGAGCACCAG CGTCACTGTC
    ACCATCCCCG ACGTGGGCCC CTTGGTGGAC TCAACCGTGG TGCACATCAG CAACAAGACT
    GATGGAGAAG CCAGTCCCTT GAAAGAGGCA GAGATCAGAG AGGATGAGGA GGAGGTGGAG
    AAGAAGCAGC AGAAGAAGGA GAAGCGTGAG ACGGGCAAGG CCATGGTGCC CCACAGCTCG
    ATGTTCATCT TCAGCACCAC CAACCCGATC CGCAGGGCCT GCCACTACAT CGTGAACCTG
    CGCTACTTCG AGATGTGCAT CCTCCTGGTG ATCGCAGCCA GCAGCATCGC CCTGGCGGCC
    GAGGACCCCG TCCTGACCAA CTCAGAGCGC AACAAAGTGC TGAGGTATTT TGACTATGTG
    TTCACTGGCG TGTTCACCTT TGAGATGGTT ATAAAGATGA TAGACCAGGG CTTGATTCTG
    CAGGACGGCT CCTACTTCCG AGACCTGTGG AACATCCTGG ACTTCGTGGT GGTGGTTGGC
    GCATTGGTGG CCTTTGCTCT GGCAACTAAC AAGGGCCGGG ATATTAAGAC CATCAAGTCC
    CTGAGGGTTC TCCGTGTCCT GAGGCCGCTG AAGACCATCA AGCGCCTGCC CAAGCTCAAG
    GCTGTCTTTG ACTGTGTGGT CACCTCCTTG AAAAACGTCT TCAACATACT CATTGTCTAC
    AAGCTCTTCA TGTTCATCTT TGCTGTCATC GCAGTTCAGC TTTTCAAGGG CAAGTTCTTC
    TACTGCACGG ACAGTTCCAA GGACACAGAG AAGGAGTGCA TAGGCAACTA TGTAGATCAC
    GAGAAAAACA AGATGGAGGT GAAAGGCCGA GAGTGGAAGC GCCATGAATT CCATTATGAC
    AACATCATCT GGGCTCTGCT GACCCTCTTC ACTGTCTCCA CAGGGGAAGG GTGGCCTCAG
    GTCTTGCAGC ACTCGGTCGA TGTGACAGAG GAAGACCGAG GCCCAAGCCG CAGCAACCGG
    ATGGAGATGT CCATCTTTTA TGTGGTCTAC TTTGTGGTCT TCCCCTTCTT CTTTGTCAAC
    ATCTTTGTGG CTCTCATCAT CATCACCTTC CAGGAGCAAG GGGACAAGAT GATGGAAGAG
    TGCAGCCTGG AGAAGAATGA GAGGGCATGC ATCGACTTCG CCATCAGCGC CAAACCGCTC
    ACCCGCTACA TGCCCCAGAA CAGGCACACC TTCCAGTACC GCGTCTGGCA CTTCGTGGTG
    TCTCCATCCT TTGAGTACAC CATCATGGCC ATGATCGCCC TGAACACCGT CGTGCTGATG
    ATGAAGTACT ACTCTGCTCC CTGTACCTAT GAGCTGGCCC TGAAGTACCT AAATATCGCC
    TTCACCATGG TGTTTTCCCT GGAATGTGTC CTGAAGATCA TTGCATTTGG CTTCTTGAAC
    TATTTCCGAG ACACCTGGAA TATCTTTGAC TTCATCACTG TGATTGGCAG TATCACAGAA
    ATTATTCTGA CGGACAGCAA GCTGGTGAAC ACTAGTGGCT TCAACATGAG CTTTCTGAAG
    CTCTTCCGAG CGGCCCGCCT CATCAAGCTG CTGCGTCAGG GCTACACCAT CCGCATTTTA
    CTGTGGACCT TCGTGCAGTC CTTTAAGGCC CTTCCCTATG TCTGCCTTCT GATCGCCATG
    CTTTTCTTCA TCTATGCCAT CATTGGGATG CAGGTCTTTG GAAACATAAA GTTAGATGAG
    GAGAGTCACA TCAACCGGCA CAACAACTTC CGGAGTTTCT TTGGGTCCCT CATGCTGCTC
    TTCAGGAGTG CCACGGGTGA GGCCTGGCAG GAGATAATGC TGTCGTGTCT CGGGGAGAAG
    GGCTGTGAGC CCGACACCAC TGCGCCGTCG GGGCAGAGCG AGAGCGAGCG CTGTGGCACC
    GACCTGGCCT ACGTGTACTT CGTCTCCTTC ATCTTCTTCT GCTCCTTCCT GATGCTCAAC
    CTGTTCGTGG CTGTCATCAT GGACAACTTT GAGTACCTGA CTCGGGACTC GTCCATCCTG
    GGGCCTCACC ATCTGGACGA GTTTGTCCGA GTCTGGGCAG AGTATGACAG AGCAGCATGT
    GGCCGCATCC ATTACACTGA GATGTATGAA ATGCTGACTC TCATGTCACC GCCGCTAGGC
    CTCGGCAAGA GATGTCCCTC CAAGGTGGCA TATAAGAGGT TGGTCTTGAT GAACATGCCA
    GTGGCCGAGG ACATGACCGT CCACTTCACT TCCACGCTGA TGGCTCTAAT CCGGACAGCT
    CTGGACATTA AAATCGCCAA AGGCGGTGCA GACAGGCAGC AGCTAGACTC AGAGCTCCAG
    AAGGAGACCC TGGCCATCTG GCCTCACCTG TCCCAGAAGA TGCTGGATCT GCTCGTGCCC
    ATGCCCAAAG CCTCTGACCT GACTGTGGGC AAAATCTATG CAGCAATGAT GATCATGGAC
    TACTACAAAC AGAGCAAGGT GAAGAAGCAG AGGCAGCAGC TGGAGGAACA GAAAAACGCA
    CCCATGTTCC AGCGCATGGA GCCCTCGTCT CTGCCTCAGG AGATCATCGC TAATGCCAAA
    GCCCTGCCTT ATCTCCAGCA GGACCCCGTT CCAGGCCTGA GTGGCCGGAG TGCATACCCT
    TCGATGAGTC CACTCTCCCC GCAGGAAATA TTCCAGTTGG CCTGTATGGA CCCAGCTGAT
    GATGGACAGT TCCAGGAACG ACAATCTCTG GAGCCAGAGG TTAGTGAATT AAAAAGCGTG
    CAGCCCTCTA ACCATGGCAT CTACCTTCCT TCGGACACCC AGGAGCATGC GGGCTCTGGG
    AAGGCATCCT CTATGCCACG CCTGACTGTG GATCCCCAGG TGGTGACAGA CCCCAGCTCC
    ATGAGACGTT CATTTTCCAC CATTCGCGAT AAGCGTTCAA ATTCCTCGTG GCTGGAGGAA
    TTCTCCATGG AGCGAAGCAG CGAAAACACC TACAAGTCCC GGCGCCGGAG TTACCACTCC
    TCCCTGAGGC TGTCAGCCCA CCGTCTGAAC TCGGACTCAG GCCACAAGTC CGACACCCAC
    CGCTCAGGGG GCAGAGAGCG GGGACGATCG AAAGAGCGAA AGCATCTTCT CTCTCCTGAC
    GTCTCCCGCT GCAACTCCGA GGAGCGAGGG ACCCAGGCCG ACTGGGAGTC CCCAGAGCGC
    CATCAGTCCC GGTCTCCCAG TGAAGGCAGG TCACAGACCC CCAACAGACA GGGCACGGGG
    TCCCTGAGTG AGAGCTCCAT CCCCTCTGTC TCCGACTCCA GCACCCCACG ACGAAGTCGG
    CGGCAGCTCC CCCCAGTCCC TCCCAAGCCT CGGCCCCTCC TTTCCTACAG CTCTCTGATC
    AGACACGCTG GGAGCATCTC CCCACCAGCT GATGGGAGCG AGGGTGGCTC CCCACTGACC
    TCCCAAGCTC TGGAGAGCAA CGACGCCGGC CTGCCCGAGT CTTCCAACCC CCGGCAGGGC
    CACCCCTCCC CACAGCGCTA CATCTCCGAG CCCTACCTGG CCCTGCACGA AGACTCCCAC
    GCCTCGGACT GCGGCGAGGA GGAGACGCTC ACCTTTGAAG CAGCCGTGGC CACCAGCCTG
    GGCCGGTCCA ACACCATCGG CTCGGCCCCG CCCCTGCGGC ACAGCTGGCA GATGCCCAAT
    GGGCACTATC GGCGGCGGAG GCGCGGGGGG CCTGGACCGG GCACGACGTG CGGAGCCGTC
    AGCGACCTCC TGAGTGACAC GGAAGAAGAC GACAAATGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223447
    Clone ID Related Accession (Same CDS sequence) XM_015475247.2
    Accession Version XM_015475247.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475247.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG
    CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG
    GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC
    AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG
    GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA
    GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG
    CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC
    AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC
    AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG
    TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT
    GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA
    TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA
    GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG
    GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC
    GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC
    AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC
    CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC
    TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG
    GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC
    ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC
    ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC
    AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC
    TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC
    CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT
    CTGGCAACTA ACAAGGGCCG GGATATTAAG ACCATCAAGT CCCTGAGGGT TCTCCGTGTC
    CTGAGGCCGC TGAAGACCAT CAAGCGCCTG CCCAAGCTCA AGGCTGTCTT TGACTGTGTG
    GTCACCTCCT TGAAAAACGT CTTCAACATA CTCATTGTCT ACAAGCTCTT CATGTTCATC
    TTTGCTGTCA TCGCAGTTCA GCTTTTCAAG GGCAAGTTCT TCTACTGCAC GGACAGTTCC
    AAGGACACAG AGAAGGAGTG CATAGGCAAC TATGTAGATC ACGAGAAAAA CAAGATGGAG
    GTGAAAGGCC GAGAGTGGAA GCGCCATGAA TTCCATTATG ACAACATCAT CTGGGCTCTG
    CTGACCCTCT TCACTGTCTC CACAGGGGAA GGGTGGCCTC AGGTCTTGCA GCACTCGGTC
    GATGTGACAG AGGAAGACCG AGGCCCAAGC CGCAGCAACC GGATGGAGAT GTCCATCTTT
    TATGTGGTCT ACTTTGTGGT CTTCCCCTTC TTCTTTGTCA ACATCTTTGT GGCTCTCATC
    ATCATCACCT TCCAGGAGCA AGGGGACAAG ATGATGGAAG AGTGCAGCCT GGAGAAGAAT
    GAGAGGGCAT GCATCGACTT CGCCATCAGC GCCAAACCGC TCACCCGCTA CATGCCCCAG
    AACAGGCACA CCTTCCAGTA CCGCGTCTGG CACTTCGTGG TGTCTCCATC CTTTGAGTAC
    ACCATCATGG CCATGATCGC CCTGAACACC GTCGTGCTGA TGATGAAGTA CTACTCTGCT
    CCCTGTACCT ATGAGCTGGC CCTGAAGTAC CTAAATATCG CCTTCACCAT GGTGTTTTCC
    CTGGAATGTG TCCTGAAGAT CATTGCATTT GGCTTCTTGA ACTATTTCCG AGACACCTGG
    AATATCTTTG ACTTCATCAC TGTGATTGGC AGTATCACAG AAATTATTCT GACGGACAGC
    AAGCTGGTGA ACACTAGTGG CTTCAACATG AGCTTTCTGA AGCTCTTCCG AGCGGCCCGC
    CTCATCAAGC TGCTGCGTCA GGGCTACACC ATCCGCATTT TACTGTGGAC CTTCGTGCAG
    TCCTTTAAGG CCCTTCCCTA TGTCTGCCTT CTGATCGCCA TGCTTTTCTT CATCTATGCC
    ATCATTGGGA TGCAGGTCTT TGGAAACATA AAGTTAGATG AGGAGAGTCA CATCAACCGG
    CACAACAACT TCCGGAGTTT CTTTGGGTCC CTCATGCTGC TCTTCAGGAG TGCCACGGGT
    GAGGCCTGGC AGGAGATAAT GCTGTCGTGT CTCGGGGAGA AGGGCTGTGA GCCCGACACC
    ACTGCGCCGT CGGGGCAGAG CGAGAGCGAG CGCTGTGGCA CCGACCTGGC CTACGTGTAC
    TTCGTCTCCT TCATCTTCTT CTGCTCCTTC CTGATGCTCA ACCTGTTCGT GGCTGTCATC
    ATGGACAACT TTGAGTACCT GACTCGGGAC TCGTCCATCC TGGGGCCTCA CCATCTGGAC
    GAGTTTGTCC GAGTCTGGGC AGAGTATGAC AGAGCAGCAT GTGGCCGCAT CCATTACACT
    GAGATGTATG AAATGCTGAC TCTCATGTCA CCGCCGCTAG GCCTCGGCAA GAGATGTCCC
    TCCAAGGTGG CATATAAGAG GTTGGTCTTG ATGAACATGC CAGTGGCCGA GGACATGACC
    GTCCACTTCA CTTCCACGCT GATGGCTCTA ATCCGGACAG CTCTGGACAT TAAAATCGCC
    AAAGGCGGTG CAGACAGGCA GCAGCTAGAC TCAGAGCTCC AGAAGGAGAC CCTGGCCATC
    TGGCCTCACC TGTCCCAGAA GATGCTGGAT CTGCTCGTGC CCATGCCCAA AGCCTCTGAC
    CTGACTGTGG GCAAAATCTA TGCAGCAATG ATGATCATGG ACTACTACAA ACAGAGCAAG
    GTGAAGAAGC AGAGGCAGCA GCTGGAGGAA CAGAAAAACG CACCCATGTT CCAGCGCATG
    GAGCCCTCGT CTCTGCCTCA GGAGATCATC GCTAATGCCA AAGCCCTGCC TTATCTCCAG
    CAGGACCCCG TTCCAGGCCT GAGTGGCCGG AGTGCATACC CTTCGATGAG TCCACTCTCC
    CCGCAGGAAA TATTCCAGTT GGCCTGTATG GACCCAGCTG ATGATGGACA GTTCCAGGAA
    CGACAATCTC TGGAGCCAGA GGTTAGTGAA TTAAAAAGCG TGCAGCCCTC TAACCATGGC
    ATCTACCTTC CTTCGGACAC CCAGGAGCAT GCGGGCTCTG GGAAGGCATC CTCTATGCCA
    CGCCTGACTG TGGATCCCCA GGTGGTGACA GACCCCAGCT CCATGAGACG TTCATTTTCC
    ACCATTCGCG ATAAGCGTTC AAATTCCTCG TGGCTGGAGG AATTCTCCAT GGAGCGAAGC
    AGCGAAAACA CCTACAAGTC CCGGCGCCGG AGTTACCACT CCTCCCTGAG GCTGTCAGCC
    CACCGTCTGA ACTCGGACTC AGGCCACAAG TCCGACACCC ACCGCTCAGG GGGCAGAGAG
    CGGGGACGAT CGAAAGAGCG AAAGCATCTT CTCTCTCCTG ACGTCTCCCG CTGCAACTCC
    GAGGAGCGAG GGACCCAGGC CGACTGGGAG TCCCCAGAGC GCCATCAGTC CCGGTCTCCC
    AGTGAAGGCA GGTCACAGAC CCCCAACAGA CAGGGCACGG GGTCCCTGAG TGAGAGCTCC
    ATCCCCTCTG TCTCCGACTC CAGCACCCCA CGACGAAGTC GGCGGCAGCT CCCCCCAGTC
    CCTCCCAAGC CTCGGCCCCT CCTTTCCTAC AGCTCTCTGA TCAGACACGC TGGGAGCATC
    TCCCCACCAG CTGATGGGAG CGAGGGTGGC TCCCCACTGA CCTCCCAAGC TCTGGAGAGC
    AACGACGCCG GCCTGCCCGA GTCTTCCAAC CCCCGGCAGG GCCACCCCTC CCCACAGCGC
    TACATCTCCG AGCCCTACCT GGCCCTGCAC GAAGACTCCC ACGCCTCGGA CTGCGGCGAG
    GAGGAGACGC TCACCTTTGA AGCAGCCGTG GCCACCAGCC TGGGCCGGTC CAACACCATC
    GGCTCGGCCC CGCCCCTGCG GCACAGCTGG CAGATGCCCA ATGGGCACTA TCGGCGGCGG
    AGGCGCGGGG GGCCTGGACC GGGCACGACG TGCGGAGCCG TCAGCGACCT CCTGAGTGAC
    ACGGAAGAAG ACGACAAATG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330733.1
    CDS808..7731
    Translation

    Target ORF information:

    RefSeq Version XM_015475247.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 E (CACNA1E), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475247.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCCGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG
    CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG
    GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC
    AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG
    GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA
    GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG
    CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC
    AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC
    AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG
    TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT
    GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA
    TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA
    GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG
    GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC
    GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC
    AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC
    CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC
    TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG
    GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC
    ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC
    ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC
    AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC
    TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC
    CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT
    CTGGCAACTA ACAAGGGCCG GGATATTAAG ACCATCAAGT CCCTGAGGGT TCTCCGTGTC
    CTGAGGCCGC TGAAGACCAT CAAGCGCCTG CCCAAGCTCA AGGCTGTCTT TGACTGTGTG
    GTCACCTCCT TGAAAAACGT CTTCAACATA CTCATTGTCT ACAAGCTCTT CATGTTCATC
    TTTGCTGTCA TCGCAGTTCA GCTTTTCAAG GGCAAGTTCT TCTACTGCAC GGACAGTTCC
    AAGGACACAG AGAAGGAGTG CATAGGCAAC TATGTAGATC ACGAGAAAAA CAAGATGGAG
    GTGAAAGGCC GAGAGTGGAA GCGCCATGAA TTCCATTATG ACAACATCAT CTGGGCTCTG
    CTGACCCTCT TCACTGTCTC CACAGGGGAA GGGTGGCCTC AGGTCTTGCA GCACTCGGTC
    GATGTGACAG AGGAAGACCG AGGCCCAAGC CGCAGCAACC GGATGGAGAT GTCCATCTTT
    TATGTGGTCT ACTTTGTGGT CTTCCCCTTC TTCTTTGTCA ACATCTTTGT GGCTCTCATC
    ATCATCACCT TCCAGGAGCA AGGGGACAAG ATGATGGAAG AGTGCAGCCT GGAGAAGAAT
    GAGAGGGCAT GCATCGACTT CGCCATCAGC GCCAAACCGC TCACCCGCTA CATGCCCCAG
    AACAGGCACA CCTTCCAGTA CCGCGTCTGG CACTTCGTGG TGTCTCCATC CTTTGAGTAC
    ACCATCATGG CCATGATCGC CCTGAACACC GTCGTGCTGA TGATGAAGTA CTACTCTGCT
    CCCTGTACCT ATGAGCTGGC CCTGAAGTAC CTAAATATCG CCTTCACCAT GGTGTTTTCC
    CTGGAATGTG TCCTGAAGAT CATTGCATTT GGCTTCTTGA ACTATTTCCG AGACACCTGG
    AATATCTTTG ACTTCATCAC TGTGATTGGC AGTATCACAG AAATTATTCT GACGGACAGC
    AAGCTGGTGA ACACTAGTGG CTTCAACATG AGCTTTCTGA AGCTCTTCCG AGCGGCCCGC
    CTCATCAAGC TGCTGCGTCA GGGCTACACC ATCCGCATTT TACTGTGGAC CTTCGTGCAG
    TCCTTTAAGG CCCTTCCCTA TGTCTGCCTT CTGATCGCCA TGCTTTTCTT CATCTATGCC
    ATCATTGGGA TGCAGGTCTT TGGAAACATA AAGTTAGATG AGGAGAGTCA CATCAACCGG
    CACAACAACT TCCGGAGTTT CTTTGGGTCC CTCATGCTGC TCTTCAGGAG TGCCACGGGT
    GAGGCCTGGC AGGAGATAAT GCTGTCGTGT CTCGGGGAGA AGGGCTGTGA GCCCGACACC
    ACTGCGCCGT CGGGGCAGAG CGAGAGCGAG CGCTGTGGCA CCGACCTGGC CTACGTGTAC
    TTCGTCTCCT TCATCTTCTT CTGCTCCTTC CTGATGCTCA ACCTGTTCGT GGCTGTCATC
    ATGGACAACT TTGAGTACCT GACTCGGGAC TCGTCCATCC TGGGGCCTCA CCATCTGGAC
    GAGTTTGTCC GAGTCTGGGC AGAGTATGAC AGAGCAGCAT GTGGCCGCAT CCATTACACT
    GAGATGTATG AAATGCTGAC TCTCATGTCA CCGCCGCTAG GCCTCGGCAA GAGATGTCCC
    TCCAAGGTGG CATATAAGAG GTTGGTCTTG ATGAACATGC CAGTGGCCGA GGACATGACC
    GTCCACTTCA CTTCCACGCT GATGGCTCTA ATCCGGACAG CTCTGGACAT TAAAATCGCC
    AAAGGCGGTG CAGACAGGCA GCAGCTAGAC TCAGAGCTCC AGAAGGAGAC CCTGGCCATC
    TGGCCTCACC TGTCCCAGAA GATGCTGGAT CTGCTCGTGC CCATGCCCAA AGCCTCTGAC
    CTGACTGTGG GCAAAATCTA TGCAGCAATG ATGATCATGG ACTACTACAA ACAGAGCAAG
    GTGAAGAAGC AGAGGCAGCA GCTGGAGGAA CAGAAAAACG CACCCATGTT CCAGCGCATG
    GAGCCCTCGT CTCTGCCTCA GGAGATCATC GCTAATGCCA AAGCCCTGCC TTATCTCCAG
    CAGGACCCCG TTCCAGGCCT GAGTGGCCGG AGTGCATACC CTTCGATGAG TCCACTCTCC
    CCGCAGGAAA TATTCCAGTT GGCCTGTATG GACCCAGCTG ATGATGGACA GTTCCAGGAA
    CGACAATCTC TGGAGCCAGA GGTTAGTGAA TTAAAAAGCG TGCAGCCCTC TAACCATGGC
    ATCTACCTTC CTTCGGACAC CCAGGAGCAT GCGGGCTCTG GGAAGGCATC CTCTATGCCA
    CGCCTGACTG TGGATCCCCA GGTGGTGACA GACCCCAGCT CCATGAGACG TTCATTTTCC
    ACCATTCGCG ATAAGCGTTC AAATTCCTCG TGGCTGGAGG AATTCTCCAT GGAGCGAAGC
    AGCGAAAACA CCTACAAGTC CCGGCGCCGG AGTTACCACT CCTCCCTGAG GCTGTCAGCC
    CACCGTCTGA ACTCGGACTC AGGCCACAAG TCCGACACCC ACCGCTCAGG GGGCAGAGAG
    CGGGGACGAT CGAAAGAGCG AAAGCATCTT CTCTCTCCTG ACGTCTCCCG CTGCAACTCC
    GAGGAGCGAG GGACCCAGGC CGACTGGGAG TCCCCAGAGC GCCATCAGTC CCGGTCTCCC
    AGTGAAGGCA GGTCACAGAC CCCCAACAGA CAGGGCACGG GGTCCCTGAG TGAGAGCTCC
    ATCCCCTCTG TCTCCGACTC CAGCACCCCA CGACGAAGTC GGCGGCAGCT CCCCCCAGTC
    CCTCCCAAGC CTCGGCCCCT CCTTTCCTAC AGCTCTCTGA TCAGACACGC TGGGAGCATC
    TCCCCACCAG CTGATGGGAG CGAGGGTGGC TCCCCACTGA CCTCCCAAGC TCTGGAGAGC
    AACGACGCCG GCCTGCCCGA GTCTTCCAAC CCCCGGCAGG GCCACCCCTC CCCACAGCGC
    TACATCTCCG AGCCCTACCT GGCCCTGCAC GAAGACTCCC ACGCCTCGGA CTGCGGCGAG
    GAGGAGACGC TCACCTTTGA AGCAGCCGTG GCCACCAGCC TGGGCCGGTC CAACACCATC
    GGCTCGGCCC CGCCCCTGCG GCACAGCTGG CAGATGCCCA ATGGGCACTA TCGGCGGCGG
    AGGCGCGGGG GGCCTGGACC GGGCACGACG TGCGGAGCCG TCAGCGACCT CCTGAGTGAC
    ACGGAAGAAG ACGACAAATG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93037
    Clone ID Related Accession (Same CDS sequence) XM_015475247.1
    Accession Version XM_015475247.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG
    CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG
    GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC
    AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG
    GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA
    GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG
    CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC
    AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC
    AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG
    TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT
    GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA
    TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA
    GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG
    GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC
    GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC
    AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC
    CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC
    TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG
    GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC
    ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC
    ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC
    AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC
    TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC
    CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT
    CTGGCAACTA ACAAGGGCCG GGATATTAAG ACCATCAAGT CCCTGAGGGT TCTCCGTGTC
    CTGAGGCCGC TGAAGACCAT CAAGCGCCTG CCCAAGCTCA AGGCTGTCTT TGACTGTGTG
    GTCACCTCCT TGAAAAACGT CTTCAACATA CTCATTGTCT ACAAGCTCTT CATGTTCATC
    TTTGCTGTCA TCGCAGTTCA GCTTTTCAAG GGCAAGTTCT TCTACTGCAC GGACAGTTCC
    AAGGACACAG AGAAGGAGTG CATAGGCAAC TATGTAGATC ACGAGAAAAA CAAGATGGAG
    GTGAAAGGCC GAGAGTGGAA GCGCCATGAA TTCCATTATG ACAACATCAT CTGGGCTCTG
    CTGACCCTCT TCACTGTCTC CACAGGGGAA GGGTGGCCTC AGGTCTTGCA GCACTCGGTC
    GATGTGACAG AGGAAGACCG AGGCCCAAGC CGCAGCAACC GGATGGAGAT GTCCATCTTT
    TATGTGGTCT ACTTTGTGGT CTTCCCCTTC TTCTTTGTCA ACATCTTTGT GGCTCTCATC
    ATCATCACCT TCCAGGAGCA AGGGGACAAG ATGATGGAAG AGTGCAGCCT GGAGAAGAAT
    GAGAGGGCAT GCATCGACTT CGCCATCAGC GCCAAACCGC TCACCCGCTA CATGCCCCAG
    AACAGGCACA CCTTCCAGTA CCGCGTCTGG CACTTCGTGG TGTCTCCATC CTTTGAGTAC
    ACCATCATGG CCATGATCGC CCTGAACACC GTCGTGCTGA TGATGAAGTA CTACTCTGCT
    CCCTGTACCT ATGAGCTGGC CCTGAAGTAC CTAAATATCG CCTTCACCAT GGTGTTTTCC
    CTGGAATGTG TCCTGAAGAT CATTGCATTT GGCTTCTTGA ACTATTTCCG AGACACCTGG
    AATATCTTTG ACTTCATCAC TGTGATTGGC AGTATCACAG AAATTATTCT GACGGACAGC
    AAGCTGGTGA ACACTAGTGG CTTCAACATG AGCTTTCTGA AGCTCTTCCG AGCGGCCCGC
    CTCATCAAGC TGCTGCGTCA GGGCTACACC ATCCGCATTT TACTGTGGAC CTTCGTGCAG
    TCCTTTAAGG CCCTTCCCTA TGTCTGCCTT CTGATCGCCA TGCTTTTCTT CATCTATGCC
    ATCATTGGGA TGCAGGTCTT TGGAAACATA AAGTTAGATG AGGAGAGTCA CATCAACCGG
    CACAACAACT TCCGGAGTTT CTTTGGGTCC CTCATGCTGC TCTTCAGGAG TGCCACGGGT
    GAGGCCTGGC AGGAGATAAT GCTGTCGTGT CTCGGGGAGA AGGGCTGTGA GCCCGACACC
    ACTGCGCCGT CGGGGCAGAG CGAGAGCGAG CGCTGTGGCA CCGACCTGGC CTACGTGTAC
    TTCGTCTCCT TCATCTTCTT CTGCTCCTTC CTGATGCTCA ACCTGTTCGT GGCTGTCATC
    ATGGACAACT TTGAGTACCT GACTCGGGAC TCGTCCATCC TGGGGCCTCA CCATCTGGAC
    GAGTTTGTCC GAGTCTGGGC AGAGTATGAC AGAGCAGCAT GTGGCCGCAT CCATTACACT
    GAGATGTATG AAATGCTGAC TCTCATGTCA CCGCCGCTAG GCCTCGGCAA GAGATGTCCC
    TCCAAGGTGG CATATAAGAG GTTGGTCTTG ATGAACATGC CAGTGGCCGA GGACATGACC
    GTCCACTTCA CTTCCACGCT GATGGCTCTA ATCCGGACAG CTCTGGACAT TAAAATCGCC
    AAAGGCGGTG CAGACAGGCA GCAGCTAGAC TCAGAGCTCC AGAAGGAGAC CCTGGCCATC
    TGGCCTCACC TGTCCCAGAA GATGCTGGAT CTGCTCGTGC CCATGCCCAA AGCCTCTGAC
    CTGACTGTGG GCAAAATCTA TGCAGCAATG ATGATCATGG ACTACTACAA ACAGAGCAAG
    GTGAAGAAGC AGAGGCAGCA GCTGGAGGAA CAGAAAAACG CACCCATGTT CCAGCGCATG
    GAGCCCTCGT CTCTGCCTCA GGAGATCATC GCTAATGCCA AAGCCCTGCC TTATCTCCAG
    CAGGACCCCG TTCCAGGCCT GAGTGGCCGG AGTGCATACC CTTCGATGAG TCCACTCTCC
    CCGCAGGAAA TATTCCAGTT GGCCTGTATG GACCCAGCTG ATGATGGACA GTTCCAGGAA
    CGACAATCTC TGGAGCCAGA GGTTAGTGAA TTAAAAAGCG TGCAGCCCTC TAACCATGGC
    ATCTACCTTC CTTCGGACAC CCAGGAGCAT GCGGGCTCTG GGAAGGCATC CTCTATGCCA
    CGCCTGACTG TGGATCCCCA GGTGGTGACA GACCCCAGCT CCATGAGACG TTCATTTTCC
    ACCATTCGCG ATAAGCGTTC AAATTCCTCG TGGCTGGAGG AATTCTCCAT GGAGCGAAGC
    AGCGAAAACA CCTACAAGTC CCGGCGCCGG AGTTACCACT CCTCCCTGAG GCTGTCAGCC
    CACCGTCTGA ACTCGGACTC AGGCCACAAG TCCGACACCC ACCGCTCAGG GGGCAGAGAG
    CGGGGACGAT CGAAAGAGCG AAAGCATCTT CTCTCTCCTG ACGTCTCCCG CTGCAACTCC
    GAGGAGCGAG GGACCCAGGC CGACTGGGAG TCCCCAGAGC GCCATCAGTC CCGGTCTCCC
    AGTGAAGGCA GGTCACAGAC CCCCAACAGA CAGGGCACGG GGTCCCTGAG TGAGAGCTCC
    ATCCCCTCTG TCTCCGACTC CAGCACCCCA CGACGAAGTC GGCGGCAGCT CCCCCCAGTC
    CCTCCCAAGC CTCGGCCCCT CCTTTCCTAC AGCTCTCTGA TCAGACACGC TGGGAGCATC
    TCCCCACCAG CTGATGGGAG CGAGGGTGGC TCCCCACTGA CCTCCCAAGC TCTGGAGAGC
    AACGACGCCG GCCTGCCCGA GTCTTCCAAC CCCCGGCAGG GCCACCCCTC CCCACAGCGC
    TACATCTCCG AGCCCTACCT GGCCCTGCAC GAAGACTCCC ACGCCTCGGA CTGCGGCGAG
    GAGGAGACGC TCACCTTTGA AGCAGCCGTG GCCACCAGCC TGGGCCGGTC CAACACCATC
    GGCTCGGCCC CGCCCCTGCG GCACAGCTGG CAGATGCCCA ATGGGCACTA TCGGCGGCGG
    AGGCGCGGGG GGCCTGGACC GGGCACGACG TGCGGAGCCG TCAGCGACCT CCTGAGTGAC
    ACGGAAGAAG ACGACAAATG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330733.1
    CDS165..7088
    Translation

    Target ORF information:

    RefSeq Version XM_015475247.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475247.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG
    CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG
    GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC
    AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG
    GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA
    GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG
    CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC
    AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC
    AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG
    TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT
    GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA
    TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA
    GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG
    GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC
    GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC
    AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC
    CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC
    TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG
    GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC
    ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC
    ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC
    AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC
    TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC
    CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT
    CTGGCAACTA ACAAGGGCCG GGATATTAAG ACCATCAAGT CCCTGAGGGT TCTCCGTGTC
    CTGAGGCCGC TGAAGACCAT CAAGCGCCTG CCCAAGCTCA AGGCTGTCTT TGACTGTGTG
    GTCACCTCCT TGAAAAACGT CTTCAACATA CTCATTGTCT ACAAGCTCTT CATGTTCATC
    TTTGCTGTCA TCGCAGTTCA GCTTTTCAAG GGCAAGTTCT TCTACTGCAC GGACAGTTCC
    AAGGACACAG AGAAGGAGTG CATAGGCAAC TATGTAGATC ACGAGAAAAA CAAGATGGAG
    GTGAAAGGCC GAGAGTGGAA GCGCCATGAA TTCCATTATG ACAACATCAT CTGGGCTCTG
    CTGACCCTCT TCACTGTCTC CACAGGGGAA GGGTGGCCTC AGGTCTTGCA GCACTCGGTC
    GATGTGACAG AGGAAGACCG AGGCCCAAGC CGCAGCAACC GGATGGAGAT GTCCATCTTT
    TATGTGGTCT ACTTTGTGGT CTTCCCCTTC TTCTTTGTCA ACATCTTTGT GGCTCTCATC
    ATCATCACCT TCCAGGAGCA AGGGGACAAG ATGATGGAAG AGTGCAGCCT GGAGAAGAAT
    GAGAGGGCAT GCATCGACTT CGCCATCAGC GCCAAACCGC TCACCCGCTA CATGCCCCAG
    AACAGGCACA CCTTCCAGTA CCGCGTCTGG CACTTCGTGG TGTCTCCATC CTTTGAGTAC
    ACCATCATGG CCATGATCGC CCTGAACACC GTCGTGCTGA TGATGAAGTA CTACTCTGCT
    CCCTGTACCT ATGAGCTGGC CCTGAAGTAC CTAAATATCG CCTTCACCAT GGTGTTTTCC
    CTGGAATGTG TCCTGAAGAT CATTGCATTT GGCTTCTTGA ACTATTTCCG AGACACCTGG
    AATATCTTTG ACTTCATCAC TGTGATTGGC AGTATCACAG AAATTATTCT GACGGACAGC
    AAGCTGGTGA ACACTAGTGG CTTCAACATG AGCTTTCTGA AGCTCTTCCG AGCGGCCCGC
    CTCATCAAGC TGCTGCGTCA GGGCTACACC ATCCGCATTT TACTGTGGAC CTTCGTGCAG
    TCCTTTAAGG CCCTTCCCTA TGTCTGCCTT CTGATCGCCA TGCTTTTCTT CATCTATGCC
    ATCATTGGGA TGCAGGTCTT TGGAAACATA AAGTTAGATG AGGAGAGTCA CATCAACCGG
    CACAACAACT TCCGGAGTTT CTTTGGGTCC CTCATGCTGC TCTTCAGGAG TGCCACGGGT
    GAGGCCTGGC AGGAGATAAT GCTGTCGTGT CTCGGGGAGA AGGGCTGTGA GCCCGACACC
    ACTGCGCCGT CGGGGCAGAG CGAGAGCGAG CGCTGTGGCA CCGACCTGGC CTACGTGTAC
    TTCGTCTCCT TCATCTTCTT CTGCTCCTTC CTGATGCTCA ACCTGTTCGT GGCTGTCATC
    ATGGACAACT TTGAGTACCT GACTCGGGAC TCGTCCATCC TGGGGCCTCA CCATCTGGAC
    GAGTTTGTCC GAGTCTGGGC AGAGTATGAC AGAGCAGCAT GTGGCCGCAT CCATTACACT
    GAGATGTATG AAATGCTGAC TCTCATGTCA CCGCCGCTAG GCCTCGGCAA GAGATGTCCC
    TCCAAGGTGG CATATAAGAG GTTGGTCTTG ATGAACATGC CAGTGGCCGA GGACATGACC
    GTCCACTTCA CTTCCACGCT GATGGCTCTA ATCCGGACAG CTCTGGACAT TAAAATCGCC
    AAAGGCGGTG CAGACAGGCA GCAGCTAGAC TCAGAGCTCC AGAAGGAGAC CCTGGCCATC
    TGGCCTCACC TGTCCCAGAA GATGCTGGAT CTGCTCGTGC CCATGCCCAA AGCCTCTGAC
    CTGACTGTGG GCAAAATCTA TGCAGCAATG ATGATCATGG ACTACTACAA ACAGAGCAAG
    GTGAAGAAGC AGAGGCAGCA GCTGGAGGAA CAGAAAAACG CACCCATGTT CCAGCGCATG
    GAGCCCTCGT CTCTGCCTCA GGAGATCATC GCTAATGCCA AAGCCCTGCC TTATCTCCAG
    CAGGACCCCG TTCCAGGCCT GAGTGGCCGG AGTGCATACC CTTCGATGAG TCCACTCTCC
    CCGCAGGAAA TATTCCAGTT GGCCTGTATG GACCCAGCTG ATGATGGACA GTTCCAGGAA
    CGACAATCTC TGGAGCCAGA GGTTAGTGAA TTAAAAAGCG TGCAGCCCTC TAACCATGGC
    ATCTACCTTC CTTCGGACAC CCAGGAGCAT GCGGGCTCTG GGAAGGCATC CTCTATGCCA
    CGCCTGACTG TGGATCCCCA GGTGGTGACA GACCCCAGCT CCATGAGACG TTCATTTTCC
    ACCATTCGCG ATAAGCGTTC AAATTCCTCG TGGCTGGAGG AATTCTCCAT GGAGCGAAGC
    AGCGAAAACA CCTACAAGTC CCGGCGCCGG AGTTACCACT CCTCCCTGAG GCTGTCAGCC
    CACCGTCTGA ACTCGGACTC AGGCCACAAG TCCGACACCC ACCGCTCAGG GGGCAGAGAG
    CGGGGACGAT CGAAAGAGCG AAAGCATCTT CTCTCTCCTG ACGTCTCCCG CTGCAACTCC
    GAGGAGCGAG GGACCCAGGC CGACTGGGAG TCCCCAGAGC GCCATCAGTC CCGGTCTCCC
    AGTGAAGGCA GGTCACAGAC CCCCAACAGA CAGGGCACGG GGTCCCTGAG TGAGAGCTCC
    ATCCCCTCTG TCTCCGACTC CAGCACCCCA CGACGAAGTC GGCGGCAGCT CCCCCCAGTC
    CCTCCCAAGC CTCGGCCCCT CCTTTCCTAC AGCTCTCTGA TCAGACACGC TGGGAGCATC
    TCCCCACCAG CTGATGGGAG CGAGGGTGGC TCCCCACTGA CCTCCCAAGC TCTGGAGAGC
    AACGACGCCG GCCTGCCCGA GTCTTCCAAC CCCCGGCAGG GCCACCCCTC CCCACAGCGC
    TACATCTCCG AGCCCTACCT GGCCCTGCAC GAAGACTCCC ACGCCTCGGA CTGCGGCGAG
    GAGGAGACGC TCACCTTTGA AGCAGCCGTG GCCACCAGCC TGGGCCGGTC CAACACCATC
    GGCTCGGCCC CGCCCCTGCG GCACAGCTGG CAGATGCCCA ATGGGCACTA TCGGCGGCGG
    AGGCGCGGGG GGCCTGGACC GGGCACGACG TGCGGAGCCG TCAGCGACCT CCTGAGTGAC
    ACGGAAGAAG ACGACAAATG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93038
    Clone ID Related Accession (Same CDS sequence) XM_015475248.1
    Accession Version XM_015475248.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6879bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA GAGGGAGCGG AGGCGCCGGC ATCACATGTC CGTGTGGGAG
    CAGCGAACCA GCCAGTTGAG GAGGCACATG CAGATGTCCA GCCAGGAGGC CCTCAACAAG
    GAGGAGGCCC CGCCCATGAA CCCCCTCAAC CCCCTCAACC CGCTGAGCCC CCTCAACCCG
    CTCAGCGCGC ACCCCAGCCT CTACCGGAGA CCCCGGGCCA TCGAGGGGCT AGCCCTGGGC
    CTGGGGCTGG AGAAGTGTGA GGAAGAGCGC GTCAGCCGTG GGGGGTCCCT CAAAGGGGAT
    GGAGGGGACT TGGCGAGCGC CCTGGACAGC CAGAGGAGCC CCCTGGCCCT GGGCAAACGG
    GAGCCACCCT GGCTGGCCCG GCCCTGTCAC GGGAACTGTG ACCCGACCCA GCCGGAGGCG
    GGCAGTGGGG AGACGGTGGT GACCTTCGGG GACCGGGCCC GGCACAGGCA GAGCCAGCGG
    CGCAGCCGGC ACCGCCGCGT CAGGACCGAG GGCAGGGAGC CCTCCTCCAC CTCCAGAAGC
    AGGTCTGCCA GCCAGGAGCG GAGTCTGGAC GAAGCTGTGC CCAGCGAGGG GGAGAGGGAC
    CGTGAGCCCA GGGCTGGCCA TGGGGGCAAA GAACCCATGA TCCAGGAGGA AGAGCGAGAG
    CGATGCCAGG ACTTACGGAG GACCAACAGT CTGATGGTGC CCAGAGGCTC CGGGCTGGCA
    GGAGCCCTGG ATGAGGCCGA CACACCCCTT GTCCCACCCC AGCCGGAGCT GGAAGTGGGG
    AAGGACGCAG CCCTGACGGA GCAGGAGCCG GAGGGCAGCA GTGAGCAGGC TCTGCTGGGG
    GACGTGAAGC TGGACATAGG CCGGGTCAGC CAGAGCGAGC CCGACCTCTC CTGCGTCACG
    GCCAACACGG ACAAGGCCAC CACCGAGAGC ACCAGCGTCA CTGTCACCAT CCCCGACGTG
    GGCCCCTTGG TGGACTCAAC CGTGGTGCAC ATCAGCAACA AGACTGATGG AGAAGCCAGT
    CCCTTGAAAG AGGCAGAGAT CAGAGAGGAT GAGGAGGAGG TGGAGAAGAA GCAGCAGAAG
    AAGGAGAAGC GTGAGACGGG CAAGGCCATG GTGCCCCACA GCTCGATGTT CATCTTCAGC
    ACCACCAACC CGATCCGCAG GGCCTGCCAC TACATCGTGA ACCTGCGCTA CTTCGAGATG
    TGCATCCTCC TGGTGATCGC AGCCAGCAGC ATCGCCCTGG CGGCCGAGGA CCCCGTCCTG
    ACCAACTCAG AGCGCAACAA AGTGCTGAGG TATTTTGACT ATGTGTTCAC TGGCGTGTTC
    ACCTTTGAGA TGGTTATAAA GATGATAGAC CAGGGCTTGA TTCTGCAGGA CGGCTCCTAC
    TTCCGAGACC TGTGGAACAT CCTGGACTTC GTGGTGGTGG TTGGCGCATT GGTGGCCTTT
    GCTCTGGCGA ATGCTTTGGG AACTAACAAG GGCCGGGATA TTAAGACCAT CAAGTCCCTG
    AGGGTTCTCC GTGTCCTGAG GCCGCTGAAG ACCATCAAGC GCCTGCCCAA GCTCAAGGCT
    GTCTTTGACT GTGTGGTCAC CTCCTTGAAA AACGTCTTCA ACATACTCAT TGTCTACAAG
    CTCTTCATGT TCATCTTTGC TGTCATCGCA GTTCAGCTTT TCAAGGGCAA GTTCTTCTAC
    TGCACGGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGATCACGAG
    AAAAACAAGA TGGAGGTGAA AGGCCGAGAG TGGAAGCGCC ATGAATTCCA TTATGACAAC
    ATCATCTGGG CTCTGCTGAC CCTCTTCACT GTCTCCACAG GGGAAGGGTG GCCTCAGGTC
    TTGCAGCACT CGGTCGATGT GACAGAGGAA GACCGAGGCC CAAGCCGCAG CAACCGGATG
    GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CCTTCTTCTT TGTCAACATC
    TTTGTGGCTC TCATCATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAAGAGTGC
    AGCCTGGAGA AGAATGAGAG GGCATGCATC GACTTCGCCA TCAGCGCCAA ACCGCTCACC
    CGCTACATGC CCCAGAACAG GCACACCTTC CAGTACCGCG TCTGGCACTT CGTGGTGTCT
    CCATCCTTTG AGTACACCAT CATGGCCATG ATCGCCCTGA ACACCGTCGT GCTGATGATG
    AAGTACTACT CTGCTCCCTG TACCTATGAG CTGGCCCTGA AGTACCTAAA TATCGCCTTC
    ACCATGGTGT TTTCCCTGGA ATGTGTCCTG AAGATCATTG CATTTGGCTT CTTGAACTAT
    TTCCGAGACA CCTGGAATAT CTTTGACTTC ATCACTGTGA TTGGCAGTAT CACAGAAATT
    ATTCTGACGG ACAGCAAGCT GGTGAACACT AGTGGCTTCA ACATGAGCTT TCTGAAGCTC
    TTCCGAGCGG CCCGCCTCAT CAAGCTGCTG CGTCAGGGCT ACACCATCCG CATTTTACTG
    TGGACCTTCG TGCAGTCCTT TAAGGCCCTT CCCTATGTCT GCCTTCTGAT CGCCATGCTT
    TTCTTCATCT ATGCCATCAT TGGGATGCAG GTCTTTGGAA ACATAAAGTT AGATGAGGAG
    AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTCAT GCTGCTCTTC
    AGGAGTGCCA CGGGTGAGGC CTGGCAGGAG ATAATGCTGT CGTGTCTCGG GGAGAAGGGC
    TGTGAGCCCG ACACCACTGC GCCGTCGGGG CAGAGCGAGA GCGAGCGCTG TGGCACCGAC
    CTGGCCTACG TGTACTTCGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG
    TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GGGACTCGTC CATCCTGGGG
    CCTCACCATC TGGACGAGTT TGTCCGAGTC TGGGCAGAGT ATGACAGAGC AGCATGTGGC
    CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCGCC GCTAGGCCTC
    GGCAAGAGAT GTCCCTCCAA GGTGGCATAT AAGAGGTTGG TCTTGATGAA CATGCCAGTG
    GCCGAGGACA TGACCGTCCA CTTCACTTCC ACGCTGATGG CTCTAATCCG GACAGCTCTG
    GACATTAAAA TCGCCAAAGG CGGTGCAGAC AGGCAGCAGC TAGACTCAGA GCTCCAGAAG
    GAGACCCTGG CCATCTGGCC TCACCTGTCC CAGAAGATGC TGGATCTGCT CGTGCCCATG
    CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC
    TACAAACAGA GCAAGGTGAA GAAGCAGAGG CAGCAGCTGG AGGAACAGAA AAACGCACCC
    ATGTTCCAGC GCATGGAGCC CTCGTCTCTG CCTCAGGAGA TCATCGCTAA TGCCAAAGCC
    CTGCCTTATC TCCAGCAGGA CCCCGTTCCA GGCCTGAGTG GCCGGAGTGC ATACCCTTCG
    ATGAGTCCAC TCTCCCCGCA GGAAATATTC CAGTTGGCCT GTATGGACCC AGCTGATGAT
    GGACAGTTCC AGGAACGACA ATCTCTGGAG CCAGAGGTTA GTGAATTAAA AAGCGTGCAG
    CCCTCTAACC ATGGCATCTA CCTTCCTTCG GACACCCAGG AGCATGCGGG CTCTGGGAAG
    GCATCCTCTA TGCCACGCCT GACTGTGGAT CCCCAGGTGG TGACAGACCC CAGCTCCATG
    AGACGTTCAT TTTCCACCAT TCGCGATAAG CGTTCAAATT CCTCGTGGCT GGAGGAATTC
    TCCATGGAGC GAAGCAGCGA AAACACCTAC AAGTCCCGGC GCCGGAGTTA CCACTCCTCC
    CTGAGGCTGT CAGCCCACCG TCTGAACTCG GACTCAGGCC ACAAGTCCGA CACCCACCGC
    TCAGGGGGCA GAGAGCGGGG ACGATCGAAA GAGCGAAAGC ATCTTCTCTC TCCTGACGTC
    TCCCGCTGCA ACTCCGAGGA GCGAGGGACC CAGGCCGACT GGGAGTCCCC AGAGCGCCAT
    CAGTCCCGGT CTCCCAGTGA AGGCAGGTCA CAGACCCCCA ACAGACAGGG CACGGGGTCC
    CTGAGTGAGA GCTCCATCCC CTCTGTCTCC GACTCCAGCA CCCCACGACG AAGTCGGCGG
    CAGCTCCCCC CAGTCCCTCC CAAGCCTCGG CCCCTCCTTT CCTACAGCTC TCTGATCAGA
    CACGCTGGGA GCATCTCCCC ACCAGCTGAT GGGAGCGAGG GTGGCTCCCC ACTGACCTCC
    CAAGCTCTGG AGAGCAACGA CGCCGGCCTG CCCGAGTCTT CCAACCCCCG GCAGGGCCAC
    CCCTCCCCAC AGCGCTACAT CTCCGAGCCC TACCTGGCCC TGCACGAAGA CTCCCACGCC
    TCGGACTGCG GCGAGGAGGA GACGCTCACC TTTGAAGCAG CCGTGGCCAC CAGCCTGGGC
    CGGTCCAACA CCATCGGCTC GGCCCCGCCC CTGCGGCACA GCTGGCAGAT GCCCAATGGG
    CACTATCGGC GGCGGAGGCG CGGGGGGCCT GGACCGGGCA CGACGTGCGG AGCCGTCAGC
    GACCTCCTGA GTGACACGGA AGAAGACGAC AAATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330734.1
    CDS163..7041
    Translation

    Target ORF information:

    RefSeq Version XM_015475248.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475248.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA GAGGGAGCGG AGGCGCCGGC ATCACATGTC CGTGTGGGAG
    CAGCGAACCA GCCAGTTGAG GAGGCACATG CAGATGTCCA GCCAGGAGGC CCTCAACAAG
    GAGGAGGCCC CGCCCATGAA CCCCCTCAAC CCCCTCAACC CGCTGAGCCC CCTCAACCCG
    CTCAGCGCGC ACCCCAGCCT CTACCGGAGA CCCCGGGCCA TCGAGGGGCT AGCCCTGGGC
    CTGGGGCTGG AGAAGTGTGA GGAAGAGCGC GTCAGCCGTG GGGGGTCCCT CAAAGGGGAT
    GGAGGGGACT TGGCGAGCGC CCTGGACAGC CAGAGGAGCC CCCTGGCCCT GGGCAAACGG
    GAGCCACCCT GGCTGGCCCG GCCCTGTCAC GGGAACTGTG ACCCGACCCA GCCGGAGGCG
    GGCAGTGGGG AGACGGTGGT GACCTTCGGG GACCGGGCCC GGCACAGGCA GAGCCAGCGG
    CGCAGCCGGC ACCGCCGCGT CAGGACCGAG GGCAGGGAGC CCTCCTCCAC CTCCAGAAGC
    AGGTCTGCCA GCCAGGAGCG GAGTCTGGAC GAAGCTGTGC CCAGCGAGGG GGAGAGGGAC
    CGTGAGCCCA GGGCTGGCCA TGGGGGCAAA GAACCCATGA TCCAGGAGGA AGAGCGAGAG
    CGATGCCAGG ACTTACGGAG GACCAACAGT CTGATGGTGC CCAGAGGCTC CGGGCTGGCA
    GGAGCCCTGG ATGAGGCCGA CACACCCCTT GTCCCACCCC AGCCGGAGCT GGAAGTGGGG
    AAGGACGCAG CCCTGACGGA GCAGGAGCCG GAGGGCAGCA GTGAGCAGGC TCTGCTGGGG
    GACGTGAAGC TGGACATAGG CCGGGTCAGC CAGAGCGAGC CCGACCTCTC CTGCGTCACG
    GCCAACACGG ACAAGGCCAC CACCGAGAGC ACCAGCGTCA CTGTCACCAT CCCCGACGTG
    GGCCCCTTGG TGGACTCAAC CGTGGTGCAC ATCAGCAACA AGACTGATGG AGAAGCCAGT
    CCCTTGAAAG AGGCAGAGAT CAGAGAGGAT GAGGAGGAGG TGGAGAAGAA GCAGCAGAAG
    AAGGAGAAGC GTGAGACGGG CAAGGCCATG GTGCCCCACA GCTCGATGTT CATCTTCAGC
    ACCACCAACC CGATCCGCAG GGCCTGCCAC TACATCGTGA ACCTGCGCTA CTTCGAGATG
    TGCATCCTCC TGGTGATCGC AGCCAGCAGC ATCGCCCTGG CGGCCGAGGA CCCCGTCCTG
    ACCAACTCAG AGCGCAACAA AGTGCTGAGG TATTTTGACT ATGTGTTCAC TGGCGTGTTC
    ACCTTTGAGA TGGTTATAAA GATGATAGAC CAGGGCTTGA TTCTGCAGGA CGGCTCCTAC
    TTCCGAGACC TGTGGAACAT CCTGGACTTC GTGGTGGTGG TTGGCGCATT GGTGGCCTTT
    GCTCTGGCGA ATGCTTTGGG AACTAACAAG GGCCGGGATA TTAAGACCAT CAAGTCCCTG
    AGGGTTCTCC GTGTCCTGAG GCCGCTGAAG ACCATCAAGC GCCTGCCCAA GCTCAAGGCT
    GTCTTTGACT GTGTGGTCAC CTCCTTGAAA AACGTCTTCA ACATACTCAT TGTCTACAAG
    CTCTTCATGT TCATCTTTGC TGTCATCGCA GTTCAGCTTT TCAAGGGCAA GTTCTTCTAC
    TGCACGGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGATCACGAG
    AAAAACAAGA TGGAGGTGAA AGGCCGAGAG TGGAAGCGCC ATGAATTCCA TTATGACAAC
    ATCATCTGGG CTCTGCTGAC CCTCTTCACT GTCTCCACAG GGGAAGGGTG GCCTCAGGTC
    TTGCAGCACT CGGTCGATGT GACAGAGGAA GACCGAGGCC CAAGCCGCAG CAACCGGATG
    GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CCTTCTTCTT TGTCAACATC
    TTTGTGGCTC TCATCATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAAGAGTGC
    AGCCTGGAGA AGAATGAGAG GGCATGCATC GACTTCGCCA TCAGCGCCAA ACCGCTCACC
    CGCTACATGC CCCAGAACAG GCACACCTTC CAGTACCGCG TCTGGCACTT CGTGGTGTCT
    CCATCCTTTG AGTACACCAT CATGGCCATG ATCGCCCTGA ACACCGTCGT GCTGATGATG
    AAGTACTACT CTGCTCCCTG TACCTATGAG CTGGCCCTGA AGTACCTAAA TATCGCCTTC
    ACCATGGTGT TTTCCCTGGA ATGTGTCCTG AAGATCATTG CATTTGGCTT CTTGAACTAT
    TTCCGAGACA CCTGGAATAT CTTTGACTTC ATCACTGTGA TTGGCAGTAT CACAGAAATT
    ATTCTGACGG ACAGCAAGCT GGTGAACACT AGTGGCTTCA ACATGAGCTT TCTGAAGCTC
    TTCCGAGCGG CCCGCCTCAT CAAGCTGCTG CGTCAGGGCT ACACCATCCG CATTTTACTG
    TGGACCTTCG TGCAGTCCTT TAAGGCCCTT CCCTATGTCT GCCTTCTGAT CGCCATGCTT
    TTCTTCATCT ATGCCATCAT TGGGATGCAG GTCTTTGGAA ACATAAAGTT AGATGAGGAG
    AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTCAT GCTGCTCTTC
    AGGAGTGCCA CGGGTGAGGC CTGGCAGGAG ATAATGCTGT CGTGTCTCGG GGAGAAGGGC
    TGTGAGCCCG ACACCACTGC GCCGTCGGGG CAGAGCGAGA GCGAGCGCTG TGGCACCGAC
    CTGGCCTACG TGTACTTCGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG
    TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GGGACTCGTC CATCCTGGGG
    CCTCACCATC TGGACGAGTT TGTCCGAGTC TGGGCAGAGT ATGACAGAGC AGCATGTGGC
    CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCGCC GCTAGGCCTC
    GGCAAGAGAT GTCCCTCCAA GGTGGCATAT AAGAGGTTGG TCTTGATGAA CATGCCAGTG
    GCCGAGGACA TGACCGTCCA CTTCACTTCC ACGCTGATGG CTCTAATCCG GACAGCTCTG
    GACATTAAAA TCGCCAAAGG CGGTGCAGAC AGGCAGCAGC TAGACTCAGA GCTCCAGAAG
    GAGACCCTGG CCATCTGGCC TCACCTGTCC CAGAAGATGC TGGATCTGCT CGTGCCCATG
    CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC
    TACAAACAGA GCAAGGTGAA GAAGCAGAGG CAGCAGCTGG AGGAACAGAA AAACGCACCC
    ATGTTCCAGC GCATGGAGCC CTCGTCTCTG CCTCAGGAGA TCATCGCTAA TGCCAAAGCC
    CTGCCTTATC TCCAGCAGGA CCCCGTTCCA GGCCTGAGTG GCCGGAGTGC ATACCCTTCG
    ATGAGTCCAC TCTCCCCGCA GGAAATATTC CAGTTGGCCT GTATGGACCC AGCTGATGAT
    GGACAGTTCC AGGAACGACA ATCTCTGGAG CCAGAGGTTA GTGAATTAAA AAGCGTGCAG
    CCCTCTAACC ATGGCATCTA CCTTCCTTCG GACACCCAGG AGCATGCGGG CTCTGGGAAG
    GCATCCTCTA TGCCACGCCT GACTGTGGAT CCCCAGGTGG TGACAGACCC CAGCTCCATG
    AGACGTTCAT TTTCCACCAT TCGCGATAAG CGTTCAAATT CCTCGTGGCT GGAGGAATTC
    TCCATGGAGC GAAGCAGCGA AAACACCTAC AAGTCCCGGC GCCGGAGTTA CCACTCCTCC
    CTGAGGCTGT CAGCCCACCG TCTGAACTCG GACTCAGGCC ACAAGTCCGA CACCCACCGC
    TCAGGGGGCA GAGAGCGGGG ACGATCGAAA GAGCGAAAGC ATCTTCTCTC TCCTGACGTC
    TCCCGCTGCA ACTCCGAGGA GCGAGGGACC CAGGCCGACT GGGAGTCCCC AGAGCGCCAT
    CAGTCCCGGT CTCCCAGTGA AGGCAGGTCA CAGACCCCCA ACAGACAGGG CACGGGGTCC
    CTGAGTGAGA GCTCCATCCC CTCTGTCTCC GACTCCAGCA CCCCACGACG AAGTCGGCGG
    CAGCTCCCCC CAGTCCCTCC CAAGCCTCGG CCCCTCCTTT CCTACAGCTC TCTGATCAGA
    CACGCTGGGA GCATCTCCCC ACCAGCTGAT GGGAGCGAGG GTGGCTCCCC ACTGACCTCC
    CAAGCTCTGG AGAGCAACGA CGCCGGCCTG CCCGAGTCTT CCAACCCCCG GCAGGGCCAC
    CCCTCCCCAC AGCGCTACAT CTCCGAGCCC TACCTGGCCC TGCACGAAGA CTCCCACGCC
    TCGGACTGCG GCGAGGAGGA GACGCTCACC TTTGAAGCAG CCGTGGCCAC CAGCCTGGGC
    CGGTCCAACA CCATCGGCTC GGCCCCGCCC CTGCGGCACA GCTGGCAGAT GCCCAATGGG
    CACTATCGGC GGCGGAGGCG CGGGGGGCCT GGACCGGGCA CGACGTGCGG AGCCGTCAGC
    GACCTCCTGA GTGACACGGA AGAAGACGAC AAATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93039
    Clone ID Related Accession (Same CDS sequence) XM_015475249.1
    Accession Version XM_015475249.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent R-type calcium channel subunit alpha-1E isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA GAGGGAGCGG AGGCGCCGGC ATCACATGTC CGTGTGGGAG
    CAGCGAACCA GCCAGTTGAG GAGGCACATG CAGATGTCCA GCCAGGAGGC CCTCAACAAG
    GAGGAGGCCC CGCCCATGAA CCCCCTCAAC CCCCTCAACC CGCTGAGCCC CCTCAACCCG
    CTCAGCGCGC ACCCCAGCCT CTACCGGAGA CCCCGGGCCA TCGAGGGGCT AGCCCTGGGC
    CTGGGGCTGG AGAAGTGTGA GGAAGAGCGC GTCAGCCGTG GGGGGTCCCT CAAAGGGGAT
    GGAGGGGACT TGGCGAGCGC CCTGGACAGC CAGAGGAGCC CCCTGGCCCT GGGCAAACGG
    GAGCCACCCT GGCTGGCCCG GCCCTGTCAC GGGAACTGTG ACCCGACCCA GCCGGAGGCG
    GGCAGTGGGG AGACGGTGGT GACCTTCGGG GACCGGGCCC GGCACAGGCA GAGCCAGCGG
    CGCAGCCGGC ACCGCCGCGT CAGGACCGAG GGCAGGGAGC CCTCCTCCAC CTCCAGAAGC
    AGGTCTGCCA GCCAGGAGCG GAGTCTGGAC GAAGCTGTGC CCAGCGAGGG GGAGAGGGAC
    CGTGAGCCCA GGGCTGGCCA TGGGGGCAAA GAACCCATGA TCCAGGAGGA AGAGCGAGAG
    CGATGCCAGG ACTTACGGAG GACCAACAGT CTGATGGTGC CCAGAGGCTC CGGGCTGGCA
    GGAGCCCTGG ATGAGGCCGA CACACCCCTT GTCCCACCCC AGCCGGAGCT GGAAGTGGGG
    AAGGACGCAG CCCTGACGGA GCAGGAGCCG GAGGGCAGCA GTGAGCAGGC TCTGCTGGGG
    GACGTGAAGC TGGACATAGG CCGGGTCAGC CAGAGCGAGC CCGACCTCTC CTGCGTCACG
    GCCAACACGG ACAAGGCCAC CACCGAGAGC ACCAGCGTCA CTGTCACCAT CCCCGACGTG
    GGCCCCTTGG TGGACTCAAC CGTGGTGCAC ATCAGCAACA AGACTGATGG AGAAGCCAGT
    CCCTTGAAAG AGGCAGAGAT CAGAGAGGAT GAGGAGGAGG TGGAGAAGAA GCAGCAGAAG
    AAGGAGAAGC GTGAGACGGG CAAGGCCATG GTGCCCCACA GCTCGATGTT CATCTTCAGC
    ACCACCAACC CGATCCGCAG GGCCTGCCAC TACATCGTGA ACCTGCGCTA CTTCGAGATG
    TGCATCCTCC TGGTGATCGC AGCCAGCAGC ATCGCCCTGG CGGCCGAGGA CCCCGTCCTG
    ACCAACTCAG AGCGCAACAA AGTGCTGAGG TATTTTGACT ATGTGTTCAC TGGCGTGTTC
    ACCTTTGAGA TGGTTATAAA GATGATAGAC CAGGGCTTGA TTCTGCAGGA CGGCTCCTAC
    TTCCGAGACC TGTGGAACAT CCTGGACTTC GTGGTGGTGG TTGGCGCATT GGTGGCCTTT
    GCTCTGGCGA ATGCTTTGGG AACTAACAAG GGCCGGGATA TTAAGACCAT CAAGTCCCTG
    AGGGTTCTCC GTGTCCTGAG GCCGCTGAAG ACCATCAAGC GCCTGCCCAA GCTCAAGGCT
    GTCTTTGACT GTGTGGTCAC CTCCTTGAAA AACGTCTTCA ACATACTCAT TGTCTACAAG
    CTCTTCATGT TCATCTTTGC TGTCATCGCA GTTCAGCTTT TCAAGGGCAA GTTCTTCTAC
    TGCACGGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGATCACGAG
    AAAAACAAGA TGGAGGTGAA AGGCCGAGAG TGGAAGCGCC ATGAATTCCA TTATGACAAC
    ATCATCTGGG CTCTGCTGAC CCTCTTCACT GTCTCCACAG GGGAAGGGTG GCCTCAGGTC
    TTGCAGCACT CGGTCGATGT GACAGAGGAA GACCGAGGCC CAAGCCGCAG CAACCGGATG
    GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CCTTCTTCTT TGTCAACATC
    TTTGTGGCTC TCATCATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAAGAGTGC
    AGCCTGGAGA AGAATGAGAG GGCATGCATC GACTTCGCCA TCAGCGCCAA ACCGCTCACC
    CGCTACATGC CCCAGAACAG GCACACCTTC CAGTACCGCG TCTGGCACTT CGTGGTGTCT
    CCATCCTTTG AGTACACCAT CATGGCCATG ATCGCCCTGA ACACCGTCGT GCTGATGATG
    AAGTACTACT CTGCTCCCTG TACCTATGAG CTGGCCCTGA AGTACCTAAA TATCGCCTTC
    ACCATGGTGT TTTCCCTGGA ATGTGTCCTG AAGATCATTG CATTTGGCTT CTTGAACTAT
    TTCCGAGACA CCTGGAATAT CTTTGACTTC ATCACTGTGA TTGGCAGTAT CACAGAAATT
    ATTCTGACGG ACAGCAAGCT GGTGAACACT AGTGGCTTCA ACATGAGCTT TCTGAAGCTC
    TTCCGAGCGG CCCGCCTCAT CAAGCTGCTG CGTCAGGGCT ACACCATCCG CATTTTACTG
    TGGACCTTCG TGCAGTCCTT TAAGGCCCTT CCCTATGTCT GCCTTCTGAT CGCCATGCTT
    TTCTTCATCT ATGCCATCAT TGGGATGCAG GTCTTTGGAA ACATAAAGTT AGATGAGGAG
    AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTCAT GCTGCTCTTC
    AGGAGTGCCA CGGGTGAGGC CTGGCAGGAG ATAATGCTGT CGTGTCTCGG GGAGAAGGGC
    TGTGAGCCCG ACACCACTGC GCCGTCGGGG CAGAGCGAGA GCGAGCGCTG TGGCACCGAC
    CTGGCCTACG TGTACTTCGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG
    TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GGGACTCGTC CATCCTGGGG
    CCTCACCATC TGGACGAGTT TGTCCGAGTC TGGGCAGAGT ATGACAGAGC AGCATGTGGC
    CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCGCC GCTAGGCCTC
    GGCAAGAGAT GTCCCTCCAA GGTGGCATAT AAGAGGTTGG TCTTGATGAA CATGCCAGTG
    GCCGAGGACA TGACCGTCCA CTTCACTTCC ACGCTGATGG CTCTAATCCG GACAGCTCTG
    GACATTAAAA TCGCCAAAGG CGGTGCAGAC AGGCAGCAGC TAGACTCAGA GCTCCAGAAG
    GAGACCCTGG CCATCTGGCC TCACCTGTCC CAGAAGATGC TGGATCTGCT CGTGCCCATG
    CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC
    TACAAACAGA GCAAGGTGAA GAAGCAGAGG CAGCAGCTGG AGGAACAGAA AAACGCACCC
    ATGTTCCAGC GCATGGAGCC CTCGTCTCTG CCTCAGGAGA TCATCGCTAA TGCCAAAGCC
    CTGCCTTATC TCCAGCAGGA CCCCGTTCCA GGCCTGAGTG GCCGGAGTGC ATACCCTTCG
    ATGAGTCCAC TCTCCCCGCA GGAAATATTC CAGTTGGCCT GTATGGACCC AGCTGATGAT
    GGACAGTTCC AGGAACGACA ATCTCTGGTG GTGACAGACC CCAGCTCCAT GAGACGTTCA
    TTTTCCACCA TTCGCGATAA GCGTTCAAAT TCCTCGTGGC TGGAGGAATT CTCCATGGAG
    CGAAGCAGCG AAAACACCTA CAAGTCCCGG CGCCGGAGTT ACCACTCCTC CCTGAGGCTG
    TCAGCCCACC GTCTGAACTC GGACTCAGGC CACAAGTCCG ACACCCACCG CTCAGGGGGC
    AGAGAGCGGG GACGATCGAA AGAGCGAAAG CATCTTCTCT CTCCTGACGT CTCCCGCTGC
    AACTCCGAGG AGCGAGGGAC CCAGGCCGAC TGGGAGTCCC CAGAGCGCCA TCAGTCCCGG
    TCTCCCAGTG AAGGCAGGTC ACAGACCCCC AACAGACAGG GCACGGGGTC CCTGAGTGAG
    AGCTCCATCC CCTCTGTCTC CGACTCCAGC ACCCCACGAC GAAGTCGGCG GCAGCTCCCC
    CCAGTCCCTC CCAAGCCTCG GCCCCTCCTT TCCTACAGCT CTCTGATCAG ACACGCTGGG
    AGCATCTCCC CACCAGCTGA TGGGAGCGAG GGTGGCTCCC CACTGACCTC CCAAGCTCTG
    GAGAGCAACG ACGCCGGCCT GCCCGAGTCT TCCAACCCCC GGCAGGGCCA CCCCTCCCCA
    CAGCGCTACA TCTCCGAGCC CTACCTGGCC CTGCACGAAG ACTCCCACGC CTCGGACTGC
    GGCGAGGAGG AGACGCTCAC CTTTGAAGCA GCCGTGGCCA CCAGCCTGGG CCGGTCCAAC
    ACCATCGGCT CGGCCCCGCC CCTGCGGCAC AGCTGGCAGA TGCCCAATGG GCACTATCGG
    CGGCGGAGGC GCGGGGGGCC TGGACCGGGC ACGACGTGCG GAGCCGTCAG CGACCTCCTG
    AGTGACACGG AAGAAGACGA CAAATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330735.1
    CDS1..6750
    Translation

    Target ORF information:

    RefSeq Version XM_015475249.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475249.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA GAGGGAGCGG AGGCGCCGGC ATCACATGTC CGTGTGGGAG
    CAGCGAACCA GCCAGTTGAG GAGGCACATG CAGATGTCCA GCCAGGAGGC CCTCAACAAG
    GAGGAGGCCC CGCCCATGAA CCCCCTCAAC CCCCTCAACC CGCTGAGCCC CCTCAACCCG
    CTCAGCGCGC ACCCCAGCCT CTACCGGAGA CCCCGGGCCA TCGAGGGGCT AGCCCTGGGC
    CTGGGGCTGG AGAAGTGTGA GGAAGAGCGC GTCAGCCGTG GGGGGTCCCT CAAAGGGGAT
    GGAGGGGACT TGGCGAGCGC CCTGGACAGC CAGAGGAGCC CCCTGGCCCT GGGCAAACGG
    GAGCCACCCT GGCTGGCCCG GCCCTGTCAC GGGAACTGTG ACCCGACCCA GCCGGAGGCG
    GGCAGTGGGG AGACGGTGGT GACCTTCGGG GACCGGGCCC GGCACAGGCA GAGCCAGCGG
    CGCAGCCGGC ACCGCCGCGT CAGGACCGAG GGCAGGGAGC CCTCCTCCAC CTCCAGAAGC
    AGGTCTGCCA GCCAGGAGCG GAGTCTGGAC GAAGCTGTGC CCAGCGAGGG GGAGAGGGAC
    CGTGAGCCCA GGGCTGGCCA TGGGGGCAAA GAACCCATGA TCCAGGAGGA AGAGCGAGAG
    CGATGCCAGG ACTTACGGAG GACCAACAGT CTGATGGTGC CCAGAGGCTC CGGGCTGGCA
    GGAGCCCTGG ATGAGGCCGA CACACCCCTT GTCCCACCCC AGCCGGAGCT GGAAGTGGGG
    AAGGACGCAG CCCTGACGGA GCAGGAGCCG GAGGGCAGCA GTGAGCAGGC TCTGCTGGGG
    GACGTGAAGC TGGACATAGG CCGGGTCAGC CAGAGCGAGC CCGACCTCTC CTGCGTCACG
    GCCAACACGG ACAAGGCCAC CACCGAGAGC ACCAGCGTCA CTGTCACCAT CCCCGACGTG
    GGCCCCTTGG TGGACTCAAC CGTGGTGCAC ATCAGCAACA AGACTGATGG AGAAGCCAGT
    CCCTTGAAAG AGGCAGAGAT CAGAGAGGAT GAGGAGGAGG TGGAGAAGAA GCAGCAGAAG
    AAGGAGAAGC GTGAGACGGG CAAGGCCATG GTGCCCCACA GCTCGATGTT CATCTTCAGC
    ACCACCAACC CGATCCGCAG GGCCTGCCAC TACATCGTGA ACCTGCGCTA CTTCGAGATG
    TGCATCCTCC TGGTGATCGC AGCCAGCAGC ATCGCCCTGG CGGCCGAGGA CCCCGTCCTG
    ACCAACTCAG AGCGCAACAA AGTGCTGAGG TATTTTGACT ATGTGTTCAC TGGCGTGTTC
    ACCTTTGAGA TGGTTATAAA GATGATAGAC CAGGGCTTGA TTCTGCAGGA CGGCTCCTAC
    TTCCGAGACC TGTGGAACAT CCTGGACTTC GTGGTGGTGG TTGGCGCATT GGTGGCCTTT
    GCTCTGGCGA ATGCTTTGGG AACTAACAAG GGCCGGGATA TTAAGACCAT CAAGTCCCTG
    AGGGTTCTCC GTGTCCTGAG GCCGCTGAAG ACCATCAAGC GCCTGCCCAA GCTCAAGGCT
    GTCTTTGACT GTGTGGTCAC CTCCTTGAAA AACGTCTTCA ACATACTCAT TGTCTACAAG
    CTCTTCATGT TCATCTTTGC TGTCATCGCA GTTCAGCTTT TCAAGGGCAA GTTCTTCTAC
    TGCACGGACA GTTCCAAGGA CACAGAGAAG GAGTGCATAG GCAACTATGT AGATCACGAG
    AAAAACAAGA TGGAGGTGAA AGGCCGAGAG TGGAAGCGCC ATGAATTCCA TTATGACAAC
    ATCATCTGGG CTCTGCTGAC CCTCTTCACT GTCTCCACAG GGGAAGGGTG GCCTCAGGTC
    TTGCAGCACT CGGTCGATGT GACAGAGGAA GACCGAGGCC CAAGCCGCAG CAACCGGATG
    GAGATGTCCA TCTTTTATGT GGTCTACTTT GTGGTCTTCC CCTTCTTCTT TGTCAACATC
    TTTGTGGCTC TCATCATCAT CACCTTCCAG GAGCAAGGGG ACAAGATGAT GGAAGAGTGC
    AGCCTGGAGA AGAATGAGAG GGCATGCATC GACTTCGCCA TCAGCGCCAA ACCGCTCACC
    CGCTACATGC CCCAGAACAG GCACACCTTC CAGTACCGCG TCTGGCACTT CGTGGTGTCT
    CCATCCTTTG AGTACACCAT CATGGCCATG ATCGCCCTGA ACACCGTCGT GCTGATGATG
    AAGTACTACT CTGCTCCCTG TACCTATGAG CTGGCCCTGA AGTACCTAAA TATCGCCTTC
    ACCATGGTGT TTTCCCTGGA ATGTGTCCTG AAGATCATTG CATTTGGCTT CTTGAACTAT
    TTCCGAGACA CCTGGAATAT CTTTGACTTC ATCACTGTGA TTGGCAGTAT CACAGAAATT
    ATTCTGACGG ACAGCAAGCT GGTGAACACT AGTGGCTTCA ACATGAGCTT TCTGAAGCTC
    TTCCGAGCGG CCCGCCTCAT CAAGCTGCTG CGTCAGGGCT ACACCATCCG CATTTTACTG
    TGGACCTTCG TGCAGTCCTT TAAGGCCCTT CCCTATGTCT GCCTTCTGAT CGCCATGCTT
    TTCTTCATCT ATGCCATCAT TGGGATGCAG GTCTTTGGAA ACATAAAGTT AGATGAGGAG
    AGTCACATCA ACCGGCACAA CAACTTCCGG AGTTTCTTTG GGTCCCTCAT GCTGCTCTTC
    AGGAGTGCCA CGGGTGAGGC CTGGCAGGAG ATAATGCTGT CGTGTCTCGG GGAGAAGGGC
    TGTGAGCCCG ACACCACTGC GCCGTCGGGG CAGAGCGAGA GCGAGCGCTG TGGCACCGAC
    CTGGCCTACG TGTACTTCGT CTCCTTCATC TTCTTCTGCT CCTTCCTGAT GCTCAACCTG
    TTCGTGGCTG TCATCATGGA CAACTTTGAG TACCTGACTC GGGACTCGTC CATCCTGGGG
    CCTCACCATC TGGACGAGTT TGTCCGAGTC TGGGCAGAGT ATGACAGAGC AGCATGTGGC
    CGCATCCATT ACACTGAGAT GTATGAAATG CTGACTCTCA TGTCACCGCC GCTAGGCCTC
    GGCAAGAGAT GTCCCTCCAA GGTGGCATAT AAGAGGTTGG TCTTGATGAA CATGCCAGTG
    GCCGAGGACA TGACCGTCCA CTTCACTTCC ACGCTGATGG CTCTAATCCG GACAGCTCTG
    GACATTAAAA TCGCCAAAGG CGGTGCAGAC AGGCAGCAGC TAGACTCAGA GCTCCAGAAG
    GAGACCCTGG CCATCTGGCC TCACCTGTCC CAGAAGATGC TGGATCTGCT CGTGCCCATG
    CCCAAAGCCT CTGACCTGAC TGTGGGCAAA ATCTATGCAG CAATGATGAT CATGGACTAC
    TACAAACAGA GCAAGGTGAA GAAGCAGAGG CAGCAGCTGG AGGAACAGAA AAACGCACCC
    ATGTTCCAGC GCATGGAGCC CTCGTCTCTG CCTCAGGAGA TCATCGCTAA TGCCAAAGCC
    CTGCCTTATC TCCAGCAGGA CCCCGTTCCA GGCCTGAGTG GCCGGAGTGC ATACCCTTCG
    ATGAGTCCAC TCTCCCCGCA GGAAATATTC CAGTTGGCCT GTATGGACCC AGCTGATGAT
    GGACAGTTCC AGGAACGACA ATCTCTGGTG GTGACAGACC CCAGCTCCAT GAGACGTTCA
    TTTTCCACCA TTCGCGATAA GCGTTCAAAT TCCTCGTGGC TGGAGGAATT CTCCATGGAG
    CGAAGCAGCG AAAACACCTA CAAGTCCCGG CGCCGGAGTT ACCACTCCTC CCTGAGGCTG
    TCAGCCCACC GTCTGAACTC GGACTCAGGC CACAAGTCCG ACACCCACCG CTCAGGGGGC
    AGAGAGCGGG GACGATCGAA AGAGCGAAAG CATCTTCTCT CTCCTGACGT CTCCCGCTGC
    AACTCCGAGG AGCGAGGGAC CCAGGCCGAC TGGGAGTCCC CAGAGCGCCA TCAGTCCCGG
    TCTCCCAGTG AAGGCAGGTC ACAGACCCCC AACAGACAGG GCACGGGGTC CCTGAGTGAG
    AGCTCCATCC CCTCTGTCTC CGACTCCAGC ACCCCACGAC GAAGTCGGCG GCAGCTCCCC
    CCAGTCCCTC CCAAGCCTCG GCCCCTCCTT TCCTACAGCT CTCTGATCAG ACACGCTGGG
    AGCATCTCCC CACCAGCTGA TGGGAGCGAG GGTGGCTCCC CACTGACCTC CCAAGCTCTG
    GAGAGCAACG ACGCCGGCCT GCCCGAGTCT TCCAACCCCC GGCAGGGCCA CCCCTCCCCA
    CAGCGCTACA TCTCCGAGCC CTACCTGGCC CTGCACGAAG ACTCCCACGC CTCGGACTGC
    GGCGAGGAGG AGACGCTCAC CTTTGAAGCA GCCGTGGCCA CCAGCCTGGG CCGGTCCAAC
    ACCATCGGCT CGGCCCCGCC CCTGCGGCAC AGCTGGCAGA TGCCCAATGG GCACTATCGG
    CGGCGGAGGC GCGGGGGGCC TGGACCGGGC ACGACGTGCG GAGCCGTCAG CGACCTCCTG
    AGTGACACGG AAGAAGACGA CAAATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01606
    Clone ID Related Accession (Same CDS sequence) NM_001191123.1
    Accession Version NM_001191123.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2011-10-22
    Organism Bos taurus(Cattle)
    Product voltage-dependent R-type calcium channel subunit alpha-1E
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03009541.1, AAFC03009542.1, AAFC03028899.1, AAFC03028900.1 and AAFC03070038.1. On Jul 17, 2010 this sequence version replaced XM_001249409.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG
    CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG
    GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC
    AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG
    GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA
    GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG
    CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC
    AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC
    AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG
    TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT
    GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA
    TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA
    GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG
    GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC
    GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC
    AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC
    CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC
    TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG
    GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC
    ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC
    ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC
    AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC
    TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC
    CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT
    CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG
    GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC
    TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC
    TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC
    ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA
    AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC
    ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG
    CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG
    ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT
    GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC
    CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC
    TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA
    TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG
    TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC
    ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC
    CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT
    CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC
    CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG
    ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC
    TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT
    CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG
    AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT
    GAGCCCGACA CCACTGCGCC ATCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG
    GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC
    GTGGCCGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT
    CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC
    ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCGCCGCT AGGCCTCGGC
    AAGAGATGTC CCTCCAAGGT GGCATATAAG AGGTTGGTCT TGATGAACAT GCCAGTGGCC
    GAGGACATGA CCGTCCACTT CACTTCCACG CTGATGGCTC TAATCCGGAC AGCTCTGGAC
    ATTAAAATCG CCAAAGGCGG TGCAGACAGG CAGCAGCTAG ACTCAGAGCT CCAGAAGGAG
    ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTGG ATCTGCTCGT GCCCATGCCC
    AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAC
    AAACAGAGCA AGGTGAAGAA GCAGAGGCAG CAGCTGGAGG AACAGAAAAA CGCACCCATG
    TTCCAGCGCA TGGAGCCCTC GTCTCTGCCT CAGGAGATCA TCGCTAATGC CAAAGCCCTG
    CCTTATCTCC AGCAGGACCC CGTTCCAGGC CTGAGTGGCC GGAGTGCATA CCCTTCGATG
    AGTCCACTCT CCCCGCAGGA AATATTCCAG TTGGCCTGTA TGGACCCAGC TGATGATGGA
    CAGTTCCAGG AACGACAATC TCTGGTGGTG ACAGACCCCA GCTCCATGAG ACGTTCATTT
    TCCACCATTC GCGATAAGCG TTCAAATTCC TCGTGGCTGG AGGAATTCTC CATGGAGCGA
    AGCAGCGAAA ACACCTACAA GTCCCGGCGC CGGAGTTACC ACTCCTCCCT GAGGCTGTCA
    GCCCACCGTC TGAACTCGGA CTCAGGCCAC AAGTCCGACA CCCACCGCTC AGGGGGCAGA
    GAGCGGGGAC GATCGAAAGA GCGAAAGCAT CTTCTCTCTC CTGACGTCTC CCGCTGCAAC
    TCCGAGGAGC GAGGGACCCA GGCCGACTGG GAGTCCCCAG AGCGCCATCA GTCCCGGTCT
    CCCAGTGAAG GCAGGTCACA GACCCCCAAC AGACAGGGCA CGGGGTCCCT GAGTGAGAGC
    TCCATCCCCT CTGTCTCCGA CTCCAGCACC CCACGACGAA GTCGGCGGCA GCTCCCCCCA
    GTCCCTCCCA AGCCTCGGCC CCTCCTTTCC TACAGCTCTC TGATCAGACA CGCTGGGAGC
    ATCTCCCCAC CAGCTGATGG GAGCGAGGGT GGCTCCCCAC TGACCTCCCA AGCTCTGGAG
    AGCAACGACG CCGGCCTGCC CGAGTCTTCC AACCCCCGGC AGGGCCACCC CTCCCCACAG
    CGCTACATCT CCGAGCCCTA CCTGGCCCTG CACGAAGACT CCCACGCCTC GGACTGCGGC
    GAGGAGGAGA CGCTCACCTT TGAAGCAGCC GTGGCCACCA GCCTGGGCCG GTCCAACACC
    ATCGGCTCGG CCCCGCCCCT GCGGCACAGC TGGCAGATGC CCAATGGGCA CTATCGGCGG
    CGGAGGCGCG GGGGGCCTGG ACCGGGCACG ACGTGCGGAG CCGTCAGCGA CCTCCTGAGT
    GACACGGAAG AAGACGACAA ATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178052.1
    CDS1..6807
    Translation

    Target ORF information:

    RefSeq Version NM_001191123.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191123.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC 
    CAGAGCAGGA ACCGGCAAGG AACCCCCGTG CCGGCCTCCG GGCCGGCGGC CGCCTACAAG
    CAGTCGAAAG CGCAGAGGGC GCGGACTATG GCTTTGTACA ACCCTATTCC CGTCCGGCAG
    AACTGTTTCA CCGTCAACAG ATCCCTGTTC ATCTTCGGAG AAGATAACAT TGTCAGGAAA
    TACGCCAAGA AGCTCATCGA TTGGCCGCCG TTTGAGTACA TGATCTTGGC CACCATCATT
    GCCAATTGCA TCGTCTTGGC CCTGGAGCAA CATCTTCCTG AGGATGACAA GACCCCAATG
    TCCCGCAGAC TGGAGAAGAC AGAACCCTAT TTCATTGGGA TCTTTTGCTT TGAAGCTGGG
    ATCAAAATCG TGGCCCTGGG GTTCATCTTC CATAAGGGAT CATACCTCCG CAATGGTTGG
    AATGTCATGG ACTTCATCGT GGTCCTCAGT GGCATCCTGG CTACTGCGGG AACCCACTTC
    AACACCCATG TGGACCTGAG GACCCTCCGG GCCGTGCGTG TCCTGAGGCC CCTGAAGCTC
    GTGTCGGGGA TACCTAGCCT ACAGATTGTG TTGAAATCCA TCATGAAGGC CATGGTGCCT
    CTCCTGCAGA TTGGCCTTCT GCTCTTCTTT GCCATCCTGA TGTTTGCTAT CATCGGCTTG
    GAGTTCTACA GTGGAAAGTT GCACCGAGCG TGCTTCATGA ACAATTCAGG TATTCTAGAA
    GGATTTGACC CCCCTCACCC ATGTGGCGTG CAGGGCTGCC CAGCTGGTTA TGAATGCCGG
    GACTGGATCG GCCCCAATGA TGGGATCACC CAGTTTGATA ACATCCTTTT TGCCGTGCTG
    ACCGTCTTCC AGTGTATCAC CATGGAAGGG TGGACCACTG TGCTGTACAA TACCAATGAT
    GCCTTAGGAG CCACCTGGAA TTGGCTGTAC TTCATCCCCC TCATCATCAT TGGATCCTTC
    TTTGTTCTCA ACCTTGTCCT GGGAGTGCTT TCTGGGGAAT TTGCCAAAGA GAGAGAGAGA
    GTGGAGAACC GGAGAGCCTT CATGAAACTG CGGCGCCAGC AGCAGATCGA GCGTGAGCTT
    AACGGGTACC GCGCCTGGAT AGACAAAGCA GAGGAAGTCA TGCTTGCTGA AGAAAACAAA
    AATGCTGGAA CGTCAGCCTT AGAAGTGCTT CGGAGGGCAA CCATCAAAAG GAGCCGGACC
    GAGGCCATGG CACGGGACTC CAGCGATGAG CACTGCGTGG ACATCTCCTC TGTGGGCACA
    CCTCTCGCCC GAGCCAGCAT CAAGAGCGCC AAGGTGGACG GGGCCTCCTA TTTCCGGCAC
    AAGGAGCGAC TTTTGCGGAT CTCTATCCGC CACATGGTTA AGTCCCAGGT GTTCTACTGG
    ATCGTGCTGA GCCTGGTGGC TCTCAACACC GCCTGTGTGG CCATCGTCCA CCACAACCAG
    CCCCAGTGGC TCACCCACCT CCTCTACTAT GCAGAGTTTC TGTTTCTGGG GCTCTTCCTT
    CTAGAGATGT CCCTGAAGAT GTACGGCATG GGGCCTCGCC TTTATTTTCA CTCTTCCTTC
    AACTGCTTTG ACTTTGGGGT CACAGTGGGC AGCATCTTTG AAGTAGTCTG GGCCATCTTC
    AGACCTGGTA CATCTTTCGG AATCAGTGTC CTGCGAGCTC TCCGGCTTCT AAGAATATTT
    AAAATAACCA AGTATTGGGC ATCCCTCCGG AATTTGGTGG TCTCCTTGAT GAGTTCCATG
    AAGTCTATCA TCAGTTTACT CTTCCTCCTT TTCCTCTTCA TTGTTGTCTT TGCTCTGCTA
    GGAATGCAGC TGTTTGGGGG CAGGTTCAAC TTTAATGATG GGACTCCTTC CGCAAACTTT
    GACACCTTCC CTGCAGCCAT CATGACTGTA TTCCAGATCC TGACGGGTGA GGACTGGAAT
    GAGGTGATGT ACAACGGGAT CCGCTCCCAG GGTGGGGTCA GCTCGGGCAT GTGGTCTGCG
    ATCTATTTCA TTGTGCTTAC CTTGTTTGGA AACTACACAC TGCTGAATGT GTTCTTGGCC
    ATCGCTGTGG ACAATCTGGC CAACGCCCAG GAGCTGACCA AGGATGAACA AGAAGAAGAG
    GAGGCCTTCA ACCAGAAGCA TGCACTGCAG AAGGCCAAGG AGGTCAGCCC GATGTCTGCA
    CCCAACATGC CTTCCATCGA AAGAGACAGA AGGAGAAGAC ACCACATGTC GATGTGGGAG
    CCACGCAGCA GCCACCTGAG GGAGCGGAGG CGCCGGCATC ACATGTCCGT GTGGGAGCAG
    CGAACCAGCC AGTTGAGGAG GCACATGCAG ATGTCCAGCC AGGAGGCCCT CAACAAGGAG
    GAGGCCCCGC CCATGAACCC CCTCAACCCC CTCAACCCGC TGAGCCCCCT CAACCCGCTC
    AGCGCGCACC CCAGCCTCTA CCGGAGACCC CGGGCCATCG AGGGGCTAGC CCTGGGCCTG
    GGGCTGGAGA AGTGTGAGGA AGAGCGCGTC AGCCGTGGGG GGTCCCTCAA AGGGGATGGA
    GGGGACTTGG CGAGCGCCCT GGACAGCCAG AGGAGCCCCC TGGCCCTGGG CAAACGGGAG
    CCACCCTGGC TGGCCCGGCC CTGTCACGGG AACTGTGACC CGACCCAGCC GGAGGCGGGC
    AGTGGGGAGA CGGTGGTGAC CTTCGGGGAC CGGGCCCGGC ACAGGCAGAG CCAGCGGCGC
    AGCCGGCACC GCCGCGTCAG GACCGAGGGC AGGGAGCCCT CCTCCACCTC CAGAAGCAGG
    TCTGCCAGCC AGGAGCGGAG TCTGGACGAA GCTGTGCCCA GCGAGGGGGA GAGGGACCGT
    GAGCCCAGGG CTGGCCATGG GGGCAAAGAA CCCATGATCC AGGAGGAAGA GCGAGAGCGA
    TGCCAGGACT TACGGAGGAC CAACAGTCTG ATGGTGCCCA GAGGCTCCGG GCTGGCAGGA
    GCCCTGGATG AGGCCGACAC ACCCCTTGTC CCACCCCAGC CGGAGCTGGA AGTGGGGAAG
    GACGCAGCCC TGACGGAGCA GGAGCCGGAG GGCAGCAGTG AGCAGGCTCT GCTGGGGGAC
    GTGAAGCTGG ACATAGGCCG GGTCAGCCAG AGCGAGCCCG ACCTCTCCTG CGTCACGGCC
    AACACGGACA AGGCCACCAC CGAGAGCACC AGCGTCACTG TCACCATCCC CGACGTGGGC
    CCCTTGGTGG ACTCAACCGT GGTGCACATC AGCAACAAGA CTGATGGAGA AGCCAGTCCC
    TTGAAAGAGG CAGAGATCAG AGAGGATGAG GAGGAGGTGG AGAAGAAGCA GCAGAAGAAG
    GAGAAGCGTG AGACGGGCAA GGCCATGGTG CCCCACAGCT CGATGTTCAT CTTCAGCACC
    ACCAACCCGA TCCGCAGGGC CTGCCACTAC ATCGTGAACC TGCGCTACTT CGAGATGTGC
    ATCCTCCTGG TGATCGCAGC CAGCAGCATC GCCCTGGCGG CCGAGGACCC CGTCCTGACC
    AACTCAGAGC GCAACAAAGT GCTGAGGTAT TTTGACTATG TGTTCACTGG CGTGTTCACC
    TTTGAGATGG TTATAAAGAT GATAGACCAG GGCTTGATTC TGCAGGACGG CTCCTACTTC
    CGAGACCTGT GGAACATCCT GGACTTCGTG GTGGTGGTTG GCGCATTGGT GGCCTTTGCT
    CTGGCGAATG CTTTGGGAAC TAACAAGGGC CGGGATATTA AGACCATCAA GTCCCTGAGG
    GTTCTCCGTG TCCTGAGGCC GCTGAAGACC ATCAAGCGCC TGCCCAAGCT CAAGGCTGTC
    TTTGACTGTG TGGTCACCTC CTTGAAAAAC GTCTTCAACA TACTCATTGT CTACAAGCTC
    TTCATGTTCA TCTTTGCTGT CATCGCAGTT CAGCTTTTCA AGGGCAAGTT CTTCTACTGC
    ACGGACAGTT CCAAGGACAC AGAGAAGGAG TGCATAGGCA ACTATGTAGA TCACGAGAAA
    AACAAGATGG AGGTGAAAGG CCGAGAGTGG AAGCGCCATG AATTCCATTA TGACAACATC
    ATCTGGGCTC TGCTGACCCT CTTCACTGTC TCCACAGGGG AAGGGTGGCC TCAGGTCTTG
    CAGCACTCGG TCGATGTGAC AGAGGAAGAC CGAGGCCCAA GCCGCAGCAA CCGGATGGAG
    ATGTCCATCT TTTATGTGGT CTACTTTGTG GTCTTCCCCT TCTTCTTTGT CAACATCTTT
    GTGGCTCTCA TCATCATCAC CTTCCAGGAG CAAGGGGACA AGATGATGGA AGAGTGCAGC
    CTGGAGAAGA ATGAGAGGGC ATGCATCGAC TTCGCCATCA GCGCCAAACC GCTCACCCGC
    TACATGCCCC AGAACAGGCA CACCTTCCAG TACCGCGTCT GGCACTTCGT GGTGTCTCCA
    TCCTTTGAGT ACACCATCAT GGCCATGATC GCCCTGAACA CCGTCGTGCT GATGATGAAG
    TACTACTCTG CTCCCTGTAC CTATGAGCTG GCCCTGAAGT ACCTAAATAT CGCCTTCACC
    ATGGTGTTTT CCCTGGAATG TGTCCTGAAG ATCATTGCAT TTGGCTTCTT GAACTATTTC
    CGAGACACCT GGAATATCTT TGACTTCATC ACTGTGATTG GCAGTATCAC AGAAATTATT
    CTGACGGACA GCAAGCTGGT GAACACTAGT GGCTTCAACA TGAGCTTTCT GAAGCTCTTC
    CGAGCGGCCC GCCTCATCAA GCTGCTGCGT CAGGGCTACA CCATCCGCAT TTTACTGTGG
    ACCTTCGTGC AGTCCTTTAA GGCCCTTCCC TATGTCTGCC TTCTGATCGC CATGCTTTTC
    TTCATCTATG CCATCATTGG GATGCAGGTC TTTGGAAACA TAAAGTTAGA TGAGGAGAGT
    CACATCAACC GGCACAACAA CTTCCGGAGT TTCTTTGGGT CCCTCATGCT GCTCTTCAGG
    AGTGCCACGG GTGAGGCCTG GCAGGAGATA ATGCTGTCGT GTCTCGGGGA GAAGGGCTGT
    GAGCCCGACA CCACTGCGCC ATCGGGGCAG AGCGAGAGCG AGCGCTGTGG CACCGACCTG
    GCCTACGTGT ACTTCGTCTC CTTCATCTTC TTCTGCTCCT TCCTGATGCT CAACCTGTTC
    GTGGCCGTCA TCATGGACAA CTTTGAGTAC CTGACTCGGG ACTCGTCCAT CCTGGGGCCT
    CACCATCTGG ACGAGTTTGT CCGAGTCTGG GCAGAGTATG ACAGAGCAGC ATGTGGCCGC
    ATCCATTACA CTGAGATGTA TGAAATGCTG ACTCTCATGT CACCGCCGCT AGGCCTCGGC
    AAGAGATGTC CCTCCAAGGT GGCATATAAG AGGTTGGTCT TGATGAACAT GCCAGTGGCC
    GAGGACATGA CCGTCCACTT CACTTCCACG CTGATGGCTC TAATCCGGAC AGCTCTGGAC
    ATTAAAATCG CCAAAGGCGG TGCAGACAGG CAGCAGCTAG ACTCAGAGCT CCAGAAGGAG
    ACCCTGGCCA TCTGGCCTCA CCTGTCCCAG AAGATGCTGG ATCTGCTCGT GCCCATGCCC
    AAAGCCTCTG ACCTGACTGT GGGCAAAATC TATGCAGCAA TGATGATCAT GGACTACTAC
    AAACAGAGCA AGGTGAAGAA GCAGAGGCAG CAGCTGGAGG AACAGAAAAA CGCACCCATG
    TTCCAGCGCA TGGAGCCCTC GTCTCTGCCT CAGGAGATCA TCGCTAATGC CAAAGCCCTG
    CCTTATCTCC AGCAGGACCC CGTTCCAGGC CTGAGTGGCC GGAGTGCATA CCCTTCGATG
    AGTCCACTCT CCCCGCAGGA AATATTCCAG TTGGCCTGTA TGGACCCAGC TGATGATGGA
    CAGTTCCAGG AACGACAATC TCTGGTGGTG ACAGACCCCA GCTCCATGAG ACGTTCATTT
    TCCACCATTC GCGATAAGCG TTCAAATTCC TCGTGGCTGG AGGAATTCTC CATGGAGCGA
    AGCAGCGAAA ACACCTACAA GTCCCGGCGC CGGAGTTACC ACTCCTCCCT GAGGCTGTCA
    GCCCACCGTC TGAACTCGGA CTCAGGCCAC AAGTCCGACA CCCACCGCTC AGGGGGCAGA
    GAGCGGGGAC GATCGAAAGA GCGAAAGCAT CTTCTCTCTC CTGACGTCTC CCGCTGCAAC
    TCCGAGGAGC GAGGGACCCA GGCCGACTGG GAGTCCCCAG AGCGCCATCA GTCCCGGTCT
    CCCAGTGAAG GCAGGTCACA GACCCCCAAC AGACAGGGCA CGGGGTCCCT GAGTGAGAGC
    TCCATCCCCT CTGTCTCCGA CTCCAGCACC CCACGACGAA GTCGGCGGCA GCTCCCCCCA
    GTCCCTCCCA AGCCTCGGCC CCTCCTTTCC TACAGCTCTC TGATCAGACA CGCTGGGAGC
    ATCTCCCCAC CAGCTGATGG GAGCGAGGGT GGCTCCCCAC TGACCTCCCA AGCTCTGGAG
    AGCAACGACG CCGGCCTGCC CGAGTCTTCC AACCCCCGGC AGGGCCACCC CTCCCCACAG
    CGCTACATCT CCGAGCCCTA CCTGGCCCTG CACGAAGACT CCCACGCCTC GGACTGCGGC
    GAGGAGGAGA CGCTCACCTT TGAAGCAGCC GTGGCCACCA GCCTGGGCCG GTCCAACACC
    ATCGGCTCGG CCCCGCCCCT GCGGCACAGC TGGCAGATGC CCAATGGGCA CTATCGGCGG
    CGGAGGCGCG GGGGGCCTGG ACCGGGCACG ACGTGCGGAG CCGTCAGCGA CCTCCTGAGT
    GACACGGAAG AAGACGACAA ATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.