gRNAs
The following CACNA1E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OBa45603 | |
| Clone ID Related Accession (Same CDS sequence) | XM_010813517.2 | |
| Accession Version | XM_010813517.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6936bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-25 | |
| Organism | Bos taurus(cattle) | |
| Product | voltage-dependent R-type calcium channel subunit alpha-1E isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_010811819.1 |
| CDS | 1..6936 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_010813517.2 |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa93040 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015475250.1 | |
| Accession Version | XM_015475250.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-25 | |
| Organism | Bos taurus(cattle) | |
| Product | voltage-dependent R-type calcium channel subunit alpha-1E isoform X5 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015330736.1 |
| CDS | 846..6119 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015475250.1 |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa216174 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001191123.2 | |
| Accession Version | NM_001191123.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6807bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-22 | |
| Organism | Bos taurus(cattle) | |
| Product | voltage-dependent R-type calcium channel subunit alpha-1E | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02043534.1, DAAA02043538.1 and DAAA02043541.1. On Jun 29, 2017 this sequence version replaced NM_001191123.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822092, SAMN02822093 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001178052.1 |
| CDS | 1..6807 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001191123.2 |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa238960 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001191123.3 | |
| Accession Version | NM_001191123.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6807bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-28 | |
| Organism | Bos taurus(cattle) | |
| Product | voltage-dependent R-type calcium channel subunit alpha-1E | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000016.1. On May 29, 2018 this sequence version replaced NM_001191123.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145414, SAMN03145415 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001178052.1 |
| CDS | 1..6807 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001191123.3 |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa223446 | |
| Clone ID Related Accession (Same CDS sequence) | XM_024976061.1 | |
| Accession Version | XM_024976061.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6702bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | voltage-dependent R-type calcium channel subunit alpha-1E isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGATGTGGG TTGATGAAGT TTGTGTATGG ATGTACATAG CCATCTTGGA GGCTGTCCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_024831829.1 |
| CDS | 7..6708 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_024976061.1 |
1 | ATGATGTGGG TTGATGAAGT TTGTGTATGG ATGTACATAG CCATCTTGGA GGCTGTCCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa223447 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015475247.2 | |
| Accession Version | XM_015475247.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6924bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | voltage-dependent R-type calcium channel subunit alpha-1E isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475247.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015330733.1 |
| CDS | 808..7731 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015475247.2 |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa93037 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015475247.1 | |
| Accession Version | XM_015475247.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6924bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-25 | |
| Organism | Bos taurus(cattle) | |
| Product | voltage-dependent R-type calcium channel subunit alpha-1E isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015330733.1 |
| CDS | 165..7088 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015475247.1 |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa93038 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015475248.1 | |
| Accession Version | XM_015475248.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6879bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-25 | |
| Organism | Bos taurus(cattle) | |
| Product | voltage-dependent R-type calcium channel subunit alpha-1E isoform X3 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015330734.1 |
| CDS | 163..7041 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015475248.1 |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa93039 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015475249.1 | |
| Accession Version | XM_015475249.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6750bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-25 | |
| Organism | Bos taurus(cattle) | |
| Product | voltage-dependent R-type calcium channel subunit alpha-1E isoform X4 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104440.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015330735.1 |
| CDS | 1..6750 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015475249.1 |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa01606 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001191123.1 | |
| Accession Version | NM_001191123.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6807bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2011-10-22 | |
| Organism | Bos taurus(Cattle) | |
| Product | voltage-dependent R-type calcium channel subunit alpha-1E | |
| Comment | PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03009541.1, AAFC03009542.1, AAFC03028899.1, AAFC03028900.1 and AAFC03070038.1. On Jul 17, 2010 this sequence version replaced XM_001249409.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. | |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001178052.1 |
| CDS | 1..6807 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001191123.1 |
1 | ATGGCTCGCT TCGGGGAGGC GGTGGTCGGC AGGCCGGGGT CGGGCGATGG AGACTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Ca(v)2.3 Ca2+ channel interacts with the G1-subunit of V-ATPase. |
A whole-genome assembly of the domestic cow, Bos taurus. |
Adrenal medulla calcium channel population is not conserved in bovine chromaffin cells in culture. |
Greater diversity than previously thought of chromaffin cell Ca2+ channels, derived from mRNA identification studies. |
